LOCUS       DAOSDS010000001        86411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_194_length_86411_cov_6.031995, whole genome shotgun sequence.
ACCESSION   DAOSDS010000001 DAOSDS010000000
VERSION     DAOSDS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 86411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 86411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86411
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_194_length_86411_cov_6.031995"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PLL88_00005"
     CDS             <1..606
                     /locus_tag="PLL88_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOI99970.1"
                     /translation="LELARIAEEHDLIVISDEIYDRLVYGVEHTCFPALPGMQKRTIL
                     VGGFSKDYAMTGWRLGYIAAPSELIKGMLLIHQYTIMTAPTMSQVAGITAILDCEGHV
                     EAMRKEYDSRRRLIVDGLNTIGLPTVEPHGAFYAFPKVNVTGLDESTFAKRLLNEKKV
                     AVVPGIAFGKAGEGFVRCSYATAADQIEEALDRIDQFVKSL"
     gene            661..2106
                     /gene="gatB"
                     /locus_tag="PLL88_00010"
     CDS             661..2106
                     /gene="gatB"
                     /locus_tag="PLL88_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOI99971.1"
                     /translation="MQYYSDIGLEVHVELSTRTKMFCGCPVVDPTVALPNTAVCPVCL
                     GMPGTLPVVNKQAVAFAIKAGLALHCQIAERSVFARKNYFYPDLPKGYQISQYELPIS
                     YEGWIAINSSQGKKTIRIRRAHLEEDTGKLNHCNQESEPHSLVDLNRAGIPLLEIVSE
                     PDMHSIEEVRAYSQELRLLLRYLGVTSGDMEKGAMRFEANVSMRANQQEALGTRVEIK
                     NLNSIHAMEKAITYELVRQQKILEEGDVVLQETLGWSENRSETFTQRSKEEAHDYRYF
                     PEPDLPPLIVERAWTESVAAEIPEFPEQKRARYQSELNLPPDKSEALIQDKAVSAYFD
                     EALKAAEDIAADTIANWIIGDLFAWLNESGCSIQEIKVCPHELVNLIRFNQQKVINKN
                     SAKEILQDMLQSGKTAQEVIHEKGMEQISSTLELEKVVTQVLQRYPAEVASYHAGKET
                     VFNWLFGQVMQATQGKADPQKVREILKKALA"
     gene            2296..3582
                     /locus_tag="PLL88_00015"
     CDS             2296..3582
                     /locus_tag="PLL88_00015"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOI99972.1"
                     /translation="MNETTNAFQMAQQQFDHVAEQLRLDPQVREYLRWPMREYHFRIP
                     VRMDDGTIKSFEGFRVQHNDSRGPCKGGIRFHPSETIDTVRALATWMTWKCAVADIPL
                     GGGKGGVIVDPATLKVSEKERLCRGYIQKIWQNIGPRLDVPAPDVGTTPQMMGWMMDE
                     YSKLVGEYTPGVITGKPVGGGGSQGRTEATGYGVIYNLREAMLRLKMDSAKCSVAIQG
                     FGNVAQYAAIGFKELLKGKVICVSYWDRHDRTSYVVSNEDGIDGRFLQSITDQYGTID
                     KEKAKTAGYKIEDGSVWLQKDVDVLIPAAIEGQINAETVLQVSDKVRIIAEGANGPTT
                     PEADEVLKKNNVFVIPDFLCNAGGVTVSYFESVQNDMNFYWTRKEVLERLDDKITEAF
                     QSVYEMSEGEKVFMRDAAYMVAINRVVTAMQLRGWI"
     gene            complement(3729..3905)
                     /locus_tag="PLL88_00020"
     CDS             complement(3729..3905)
                     /locus_tag="PLL88_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI99973.1"
                     /translation="MNLRRIARHFGIHHRTVSLWVQASAVSLPEVPLPKEVKAAEMDE
                     LLTFVGNKKTGSTS"
     gene            complement(3951..4643)
                     /locus_tag="PLL88_00025"
     CDS             complement(3951..4643)
                     /locus_tag="PLL88_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOI99974.1"
                     /translation="MFATNKDFSQYKKHQITLFKEMLHTYPKNQFDEQALPSYTNPNP
                     LMRYLFWERIRFVIKYLSSFNKINTCLDFGCGLGTMIPYLSQKVQSLFVLDLDISLLQ
                     EIGEKEGWQNVAYLDSLEQLNGFEGKIDFILALDVLEHVNNLSEILLCFSELLTKDGF
                     ILVSGPTENIFYKIGRRLANYSGHYHTRNVYDVKDEMEKHFLVSSLKTLYKPIPFFEI
                     YVAKRYIPKNKR"
     gene            5043..6170
                     /gene="dnaN"
                     /locus_tag="PLL88_00030"
     CDS             5043..6170
                     /gene="dnaN"
                     /locus_tag="PLL88_00030"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOI99975.1"
                     /translation="MKLSVSQPQLAHGLGIVTRAVSPRSTLPVLNNILLATDEGRLRL
                     SATNLELGITKWIGAQIQEEGSITIPARIFSDLVGALPGTTVELTLNPRTQTLNVHCG
                     TSNFDIKGIDAQEFPPMPVPDLQNSVDLNVSDFKEMIQQVSFAASTDEARPVLQGVMM
                     EIGNNEVTLAATDGFRISVKKAALSTPLAYPVKVIIPARSLSELARIATNGDESINMV
                     VPDGRGQVIFHLKDAELVSQLIDGKFPDYNAIIPRSFKTTTTLTTVEFQKACRQAEII
                     ARESNNVIRLHIQPQEQGPGIVEFSSQSEERGSGEVVLEANIDGPELLIAFNVSYLLN
                     VLDVVSTPNTILSTNADNTPGLIRPVSDDSFLHVIMPMHLG"
     gene            6314..8647
                     /locus_tag="PLL88_00035"
     CDS             6314..8647
                     /locus_tag="PLL88_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOI99976.1"
                     /translation="MQDFSNSTDRLMSITLAMGQYPILSDRIHSKMRQELFRRNIITQ
                     VALESEVREKAIQSQEREGVNNPLGEESAEMWEKRTTRVRDQLTDLLFSQHFSFDLFQ
                     EIVTRVLVESGISTERHNIDFNPELAPQELVFEHAWSILRLPPDEQKQYNHHLEESVV
                     VLIRTMISDQLPYINIAKRWFTIQDLSEIRKRKIGTGRIGGKAAGMLLAHRILENELD
                     DDLKPLLAKPDYYFIGSNEQYSFITMNRFERWNDQKYKTEEQMREDYPKIVDDFLKGK
                     FPNDILEKLEVLLQQVGRSPLIVRSSSLLEDSFGTSFAGKYDSFFLPNQGTPEENLSA
                     LTQAITKVWASTLNPNALLYRRKKGLQDYDERMAILIMKVEGERFGKYYLPDAAGVAF
                     SRNLYRWTPQIRREDGFVRLVWGLGTRAVDRVGNDYPRLIALSHPLLRPMPDFETVGR
                     CSQKFVDLIDMEKNAFSTLPIHEVISSDYLPLKYIAQLSEEGYFSPMHSRLMEKDRNR
                     IVLTFDELLRKTNFAVSIRKILQKLETNYNAPVDMEFTLNIEEQEAGNPTIQYTILQC
                     RPQSHLSDSPEGQIPANLQEKDILFSTYFMVPQGMVTNITHAVFVPPEEYYKLDESGR
                     FKLARAIGQLNSKLAEKQYVLIGPGRWGSTNTDLGVPVAYSDIFNTAALIELSGKDIG
                     AAPEPSLGTHFFQDLLEAQIYPLAILVDDPENVFQKSFFYHVPNRLGDYLPADDWLKN
                     ILRLLPISTYRKGNHLKIIMDEEKSYAAGYLEPDKKE"
     gene            complement(8718..10319)
                     /locus_tag="PLL88_00040"
     CDS             complement(8718..10319)
                     /locus_tag="PLL88_00040"
                     /inference="COORDINATES: protein motif:HMM:NF021601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HOI99977.1"
                     /translation="MKKDKRKVEKKSSLLLVIFCLILVASCSRAKIPAVNQITIAESG
                     DLSTRKKVVVLYDDDPADPWNFGKIYAAMVRNLLGHFEREVTILDIADYRAGALANFS
                     AGIYIGNIYDYPLSDDFLQDVISTDTPFLWMNSNIWQLFRNEEWGASSKFGFEYVEAE
                     IVNPDIKIIYKGKELPRLETDVYFNLVNIDAGSQCEQLASLQIKGAVDQPYAIRCGNF
                     FYITHNPMANFYASYLVFADLLHDLLETTVQENHRALVRFEDLMPGNTNYERVKEQVD
                     FLYEKGIPFAFGVIPVNADPEGIWGEPGKTVYLYEDFMLQELIDYMVERGGTPVLHGY
                     THQHDSVSGVDYEFWDGENDAPFPEDGYSWADERIKAGVEQFEKAMGYAPVIWETPHY
                     SASPNTYFAIKNSFDVIYERVQVFNDLNEATADSNNDYSKMPYITQAFPYQIFDSIYG
                     VRILPENLGYLEEGGNAEFGVPPTPEGKLALADMYSVVRDGVVSFMFHHWQPSQAFYD
                     TINGLEAMGYTFVSVSDLLQETAVE"
     gene            complement(10338..11162)
                     /locus_tag="PLL88_00045"
     CDS             complement(10338..11162)
                     /locus_tag="PLL88_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOI99978.1"
                     /translation="MNIGNSFTIAQKDWREVRQNKSVFIPILIVPLIFIVIFPLLITL
                     ILPNVAGAVEGFATDPDMQVFFASMPAEMKRVLDGLDAAQIVIVTVLGYIFAPMFLMV
                     PLMFSSTIAAESFAGEKERKTMEALLYLPISDLELFLGKVMAAGIPAVLITWVCFFLY
                     TIIVNGVPYASFGRLWFPLPVWWLLIFWVSPALIAMSISLTVLISTKAQTFQGAYQTS
                     SSIVLIVVVFFIGQMTGVLFLSPLVALIMGFVFWGIAAIIGRVGVKSFNRQKLLTD"
     gene            complement(11166..12098)
                     /locus_tag="PLL88_00050"
     CDS             complement(11166..12098)
                     /locus_tag="PLL88_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI99979.1"
                     /translation="MDVINLNQVSFQYGKGFALRNVTFRMKKGEIFALLGPNGAGKTT
                     SIRLVNGLLHASSGSISVLGCDPYKDGVTVRRRTGVLTETPALYERLTALENLTFFGK
                     LNGMSKTDLNTRIQELLDYFDLSAHANQRVSTYSRGMRQRLAIARALLHHPELLFLDE
                     PTSNLDPEIARQVQDLILKVKREEGCSVVICTHRLYEAEQVCDHAAIMHEGKMLAEGT
                     LEALREAAGLAKRVHFSFQKPLPAGIGGELSRLEAVKNAEVKEKELLLDVSDDERVPE
                     ILTWMIGKGLAVLSVIPQVPSLEEVYFKLQGLVR"
     gene            12299..13189
                     /gene="pdxS"
                     /locus_tag="PLL88_00055"
     CDS             12299..13189
                     /gene="pdxS"
                     /locus_tag="PLL88_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HOI99980.1"
                     /translation="MSAEEKGTFKLKAGLAQMLKGGVIMDVVTPEHAKIAEDAGAVAV
                     MALERVPADIRMQGGVARMSDPELILKIMDTVSIPVMAKARIGHFVEAQILEALGIDY
                     IDESEVLTPADEEHHINKHAFKVPFVCGCRNLGEALRRVGEGAAMIRTKGEAGTGDVV
                     EAVRHARRVLGEIRTLQNLPDEEVMAYAKELGAPYELMMETRALGHLPVVNFAAGGIA
                     TPADAALMMQLGVDGVFVGSGIFKSGDPAKRAAAIVKATTHYNDAKILAEVSRNLGEP
                     MVGRQVSSIPEQELIADRGW"
     gene            13199..13777
                     /gene="pdxT"
                     /locus_tag="PLL88_00060"
     CDS             13199..13777
                     /gene="pdxT"
                     /locus_tag="PLL88_00060"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HOI99981.1"
                     /translation="MKIGVLALQGSFYEHQQMLQNLGVQVVQVRLPHELEGLDGLIIP
                     GGESTTIGKLAVDFGLLEPLQRFGKEHAIWGTCAGSIFLSKDAHRQQPLLGLMDIQVE
                     RNAFGRQVDSFEIDLEIPVLRSVNHAKPIFHGIFIRAPLIEKAAEGVEILARLPNDKI
                     VAARQGHLLATSFHPELTDDTRFHQYFLEVVP"
     gene            13897..14691
                     /locus_tag="PLL88_00065"
     CDS             13897..14691
                     /locus_tag="PLL88_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI99982.1"
                     /translation="MKNCFLSKESIFSYRAQEDGAPAFAQLQFGERSAVFLYYISLPG
                     TSPANSIESLCRIAGEAGKAHLLANAPMHSEQWQFLHAMGFHTYSVQNIWQVNDLPAA
                     RAFSGQWCIEKDQDRSAITSYYSHFLSPLELAFQTWNFPDIFHLILHDHSACVRGVAR
                     IRFFVDRAVILPMLDLRENDEGEDDCMIALLKECSRYFSKLFIRESTRHPINTNIFAK
                     REETCLTEDHFMVRNLVAMNAIRNYQPLELLDEKGIAKPTTPFSHS"
     gene            14750..16354
                     /locus_tag="PLL88_00070"
     CDS             14750..16354
                     /locus_tag="PLL88_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOI99983.1"
                     /translation="MKQNHITDDLSALLSVLPSEFNRALERENQNDDLLEIIMDLGRV
                     PVARYIDHELTLSEKEITHDMIQDTVSRIGEFDGDNRAGIERTLHRISAIRNKKGDII
                     GLTLRVGRAVYGTIDIIEDLVKSEKSILLLGKPGIGKTTMLREAARIRAENSRVVIVD
                     TSNEIGGDGDVPHPAVGRARRMQVSNPLLQHEVMIEAVENHNPEVIVIDEIGREMEAL
                     AARTIAERGVQLIATAHGRTLENLLLNPTLSDLIGGIESVTLSDEEARRRGTQKTVLE
                     RRAPPTFDVLIEIQDRDHLAIHQDVAASVDALVRGYPLEPQIRMRDESGKINTQEPQQ
                     RVETANAQAQGLRRQNNNGSYGNEEKQLRSSNRRFESAAKEQQALLPPINVFPFGVAR
                     NRLLEAIRRLGLPAKVVHDLQDADVFITLKSYYRSRQRPILQAEERGIPIYVLRANSE
                     NQMEQALAEIFNLGGPSGRPLNYDRISQEVHNAVDLVLAGKHWVDLPPASAKIRRIQH
                     ELVREAELTSHSYGKEPNRYVRVFRE"
     gene            16360..16974
                     /gene="tmk"
                     /locus_tag="PLL88_00075"
     CDS             16360..16974
                     /gene="tmk"
                     /locus_tag="PLL88_00075"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOI99984.1"
                     /translation="MFITLEGPDGSGKTQQIQLLSAFLESRGQKVFFSREPGGTEIGD
                     KIRSILMDMHNTSMHPRTETLLFCASRAQIVEEVIKPKLQAGYIVILDRYADSTIAYQ
                     GYGHGHDLTMIRQLLAFATGGLKPNLTILLDLDPQVGLERRKQGGGEWNRMDAYQLAL
                     HKRVRAGYLEMAAQEPNRWHIVSADQPVEVVQSSIQRIILDYLE"
     gene            17008..18261
                     /locus_tag="PLL88_00080"
     CDS             17008..18261
                     /locus_tag="PLL88_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOI99985.1"
                     /translation="MRKYMVVFLLLAILFTAACSKTGVADATQVASETAGDSEAAEAT
                     VAPFTSADESCKPYNLIDDLLLQPVDANVPAVTDADFIEGPADAKYTITVYSNFTCSH
                     CANLEPILAQLQTLFSNTVRIVFRYVTTGGNSNVAAQAVEAANIQGKFQEMKNVLFEN
                     QATWYSYSEDDFRDWLGEQAASFGMDVDQFDTDIDSETTLNKVTENRTGVDELGIYGT
                     PTIYVNNRQYTQTRSVATFAIMLDVMDISDQLLGDCPIISTDFTKDLQAVISTSKGDI
                     VVDLYEDEAPNTVAYFQYLAEKGWYNNNNIIISTAEYSISGDPSNTLYGGPGFAYFNE
                     LATDQTLNEEGLLVSFNQLGTGYNTGIFMLTKSAVTDFTGEISIFGKVIEGMDVLNAF
                     TDRPYSLDPAEPFYDSITSITIFEK"
     gene            18258..19517
                     /locus_tag="PLL88_00085"
     CDS             18258..19517
                     /locus_tag="PLL88_00085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HOI99986.1"
                     /translation="MIADERRNRDWLKISLFIFSLTAWLFTTVSAASCFAGGIVFRAT
                     LGLSSDQTAMVLSLGSLSVLLSVIHFSALRHAFKNSPPAPMPTRSDKALSISSAAMLC
                     WILVMGISYFNGNDKLFTAIQPYLTPLAIWIPIWWFIEFGKRKLPSITARKRNAALSI
                     GSSYVIVFIMALELIVFALLIAGLLIYLNTQPSIQQWIRTLSIPEDLSQIDTRFIEQY
                     IHRILQNPWFLAIVFMVIGVIAPFIEESLKPAALWVLQKRSLTPSQGFILGLYFGGAF
                     ALIENAGMVIQLGSQSWSESVLLRAATALLHITCSGLVGCGYARSMQPGKTSAFLKPF
                     LLALLLHGAWNSMAVLSGYAFFSSETGIDALSSTPALKLISIIALVLIWLIIVYLLWK
                     KNQDLRKETSFEAHTNSADGVSMNKVE"
     gene            19534..20193
                     /locus_tag="PLL88_00090"
     CDS             19534..20193
                     /locus_tag="PLL88_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOI99987.1"
                     /translation="MKRSTLQHIVRFLMRVLTSTEFLGLENIPTEGGAIIAINHLSYI
                     DIPLLFVNPRRPDITALITTKYKSHPFIRWFSETAEGIWIDRDIADFTAIREASLALA
                     QGKALGISPEGTRSLSHTLAEGKQGTAMLALKSGVPIVPTGIQGTEDAIAKLKHLRKP
                     HMVARFGKPFTLPPITEFDNRAEILQRYTEEIMCRIAVLLPEKYRGAYKDHPRLKEFA
                     I"
     gene            20195..21727
                     /locus_tag="PLL88_00095"
     CDS             20195..21727
                     /locus_tag="PLL88_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOI99988.1"
                     /translation="MPKLVLLWISASFLVGIITAGVAPLSFNWSLGFLLPLVFFIFYG
                     LGKKRENLKRWLFFALLLFSFFGGALRYQRFSEYPTLPGDASTTAEAVVLRGSVVEPS
                     EASIQYSEVVLEIQQVVDPSEGWQPAEGKVLIRLPEEFFFLYRTALTLQGTLIPAVAP
                     GEKAHTSWLEQNGIHYQMFYPTVLEETPFEGFSLMGVLHAFHGKAHRLLQAIMPYPES
                     ELLAGILLGIESRIPEHLREAYRLTGTAHILAISGFNIVLIAGMVSRFLNRLLPYRIA
                     AMFSIVVIALYAIFVGAQPAVLRAALMGIISIPAYLIGRRVIGIHSLSITAALMALFN
                     PYLLWNVSFQFSVCATLGIMMYADFFTRKADGWLQKSSVPSKEFVLGFIKDSVIITFS
                     AQVATLPVLASQFEEVSLISPLVNLLILPLQPSVAILGGLALVGGWVFYPMGRLIGMA
                     AWLLSAFTDQIILFFSQIPLTVSLNPVWGGWLSVGLNGLLIFTVLRDRKRATLQPANI
                     QG"
     gene            complement(21724..23007)
                     /locus_tag="PLL88_00100"
     CDS             complement(21724..23007)
                     /locus_tag="PLL88_00100"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOI99989.1"
                     /translation="MEKASDHLNITPQEKVDFFLRTSAHAQNEQNIEAKLSKIIDEIV
                     RFTAADQGYLFMVENEEIIPFIKRTRNGTDVEQKKVTLPTMILIQSFQANQIFFVQDA
                     QTDERFKTLLSVSAFHLHSFLCIPIFVNDKKEGLLYLQQTETKEPFLRDHLESIKVVM
                     PVIGLLLENMNLQKDALEKGRMQRELQMARDVQNGLIMKEAPHYQGWKTAIYWQPAFE
                     VGGDFYDFIPISEHLVGLVIADVSDKGMPAALFMALTRTTMRAVAMNRKNVAEMITQA
                     NHLIYHDAHENMFVTLFFGLLNLENGELFYVNAGHNPPFLYDREHDTLMKLKPTGMAM
                     GVDAQAKYEQKKATLREGDFLFLYTDGALDAVVRNKVFDEKELHAFIRRHRNADPQMI
                     VNMLKEKLYAVNSDPSYHDDLTIIAWKRDGKEKTS"
     gene            23123..23545
                     /locus_tag="PLL88_00105"
     CDS             23123..23545
                     /locus_tag="PLL88_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOI99990.1"
                     /translation="MDWLKGALRFETRGKGLYEITDQVRSAIHEWNVQEGICFLFLQH
                     TSASLTTCEAYDPSARRDVEEFYERLAPERQNWYQHTLEGSDDSPSHIRATLTQSSLS
                     IPIDDSQLSLGTWQGIFLFEHRSHPQHRQLLIRCLKTS"
     gene            23547..24470
                     /locus_tag="PLL88_00110"
     CDS             23547..24470
                     /locus_tag="PLL88_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOI99991.1"
                     /translation="MSNKDPLFITKTKVIFAVILWGGSFIATKMTVMEATPVAVVWLR
                     FLIGLLCLFPIMLKKGMLKISSWKEVGEYIVLGFVGISLHQWLQSNGLVTSQASTTAW
                     IVASTPLFMVLFGWLFLKEKLTPLSGLGVVLATLGVLTVVSGGDIAGLFQNGFGAPGD
                     LLIMLSSPNWAIYSILLRKTLQREPAVKTTFFSILFGWLLTSVQFLVGAEWQSFATLS
                     LSGWAGIFYLGIFCTFLAYIFYYDALQKLPSASVGAYLYLEPIATTLMASAVFHEPIT
                     LVSLLGGALILFGISLVNKITPREISSIEQV"
     gene            24544..25254
                     /locus_tag="PLL88_00115"
     CDS             24544..25254
                     /locus_tag="PLL88_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HOI99992.1"
                     /translation="MSIKEKIIRIIEVVSDDQQILLDNLTEKERSETGSVKRWTFKDV
                     IVHSTFWWNVFLDRLQAAAQGKQAKEMPEDINPINDAVLEEHKHDSWEVVLKENTRVR
                     QEVLKWLEKLSEEDLTDTGKYEWMRGRALYHQFLGDCWHDEWHFARYLAEHDRLEEGV
                     AIQENFVAQLKILPEWEYVAIYNLACFYSVSGMKDKAIATLKKALKMNPEMKKWSKED
                     PDFENIRNDPEYKKIYEE"
     gene            complement(25284..25859)
                     /locus_tag="PLL88_00120"
     CDS             complement(25284..25859)
                     /locus_tag="PLL88_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOI99993.1"
                     /translation="MLEKEPTFERVDPRVKRTRALITSAFMELLSEKEFQAISVQDIT
                     ERAEINRSTFYAHYVDKHALLETCIAQTFRQELETKTLNACHYSRENLRALIITVCEF
                     IAQMNPHRIASDTQFEVLVEKQVRNEIKKLLELWLVRVPSKTDPATSITAASWAIYGL
                     ALQWNQDKQKVKVDSFVNQILPQINTILQLQ"
     gene            25994..26623
                     /locus_tag="PLL88_00125"
     CDS             25994..26623
                     /locus_tag="PLL88_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOI99994.1"
                     /translation="MVKSNLVPNMQYARLASREQIERTAKALEKNGIHTLIAENGEEA
                     KNKLFESIPTGAEVFTATSRTLDTLDIPEGVNQRYNSVRSKLASMNNQTQGREMVKLG
                     ATPEYTVGSVHAVTEDGTVVIASNTGSQLAGYAASAAHVVWVVGAQKIVPNLEEAIKR
                     IYEYTLPLEDERALQTYGMNSNVSKLLIVHKEVMPGRTTLILVNENLGF"
     gene            complement(26684..27109)
                     /gene="arfB"
                     /locus_tag="PLL88_00130"
     CDS             complement(26684..27109)
                     /gene="arfB"
                     /locus_tag="PLL88_00130"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOI99995.1"
                     /translation="MIEITHSFYFDESEIRYVFYHSSGPGGQNVNKVATAVQLRFDAV
                     RSPSLPEAVRERLMKLAGKRMTKEGELIITSSRHRTQEANKEDALDKLLVILRQAAQP
                     PRTRRATTPSRASVERRLHHKRQQALKKRERRWYMGNKE"
     gene            complement(27203..27883)
                     /locus_tag="PLL88_00135"
     CDS             complement(27203..27883)
                     /locus_tag="PLL88_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOI99996.1"
                     /translation="MSDILVECWQLGKDYGDSMRTVRAVKDVSLQLKKGSFTAIMGPS
                     GSGKTTLLNLIGTLDVPTRGKLFFEGVAVDSLNDDQLADFRRENIGFVFQLFNLIPSL
                     NVMENVMIPLLPFRKALSFDLEDRARELVEMIGLGERFDHLPGELSGGEQQRVAIARA
                     MINYPKLILADEPTGNLDTMTGQEVMNVLKRMKKNQGVTILLVTHDANVASHADEIFN
                     IKDGMIVL"
     gene            complement(27880..32040)
                     /locus_tag="PLL88_00140"
     CDS             complement(27880..32040)
                     /locus_tag="PLL88_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOI99997.1"
                     /translation="MKFKNSTNHFKFKQILKLSWLQVAGVFIICCGISLFSAMLHTVE
                     VTSTAEVAEHWRGKYDILVRPASSVSDVERDTQLVEGNYLGSSYGGITDEQYALIKGI
                     DEVEVAAPVATVGYFVNSTGAVFVRLDPGEVNTLYRFTFTWSGDDPTLQNEEAQIAYF
                     TTTDYFKNYFMQGNLAIDLTMSPAQQWALMNIGIIPSQWVLVAGIDPEQEAQLIGLDT
                     ALLAGDYLEAAPLEEALESRTTKKNKVPLIINNQNYTELGISMKIEALPLEGSTETLK
                     IIQTEGGEAWQTSLRQIYDARLDAEGQLLVDEQLDLSVGRKPLAIQSVVFNTSSAPEI
                     SSDLPGSAYTTGGGVNIDRDIALLPGSYRYEAQTQSPFSDGQPLFKLEAYGSWGEMLG
                     SELEVSREGWSGRYYENLPLIPDDTMLYRPLQVVQQKDFMFDVKGIYDLSVIHDSIDE
                     LTYAPLGIYDPPMAELQYDESAQPLAAQTILPGLNPGAVVTRPPLALTNLQGARYLSG
                     REDYIDAIRVRVSGIEEYSEENLQKVERVASEISERTGLHVDVIAGSSPRNVWVYVPG
                     MGYLEEAWTTLGAVQHITQGINAMNIALLLAFLIVSGVFIMFTTNVSLMSRIEEIGVL
                     RSVGWRKADVFRYLLSELIAIGLVGAIFAAGFSLLLTSALEMDPSPFPIIGTALAVPL
                     LYTVSGIPAIRRVVQKTPCELLQRGEFTHQPQTEKRPGKKLTFSRFFFNQFSRRKNRN
                     VFTSLTFSAGIALLNLVVNILLNLQGILQVTLLGQFIALRLRNYHFVIVGIALVMGML
                     VMYENLLLGIREREDEFKFLHAAGWKKRHISGWIVLESVFLSGMGGIVGSALGTLIFW
                     LFSKTFTTNMLLIFVASLLGSLLIGIIIAVQPARRIPRRLFLRYKVNGEGTGKKTNPL
                     LLMTLLLVVLAGIIFWMGRGSLIPQSWARSTPAEEKLYDQFGIDGAALMTTIENISGE
                     SGEALSGSAPSKTVAEIIQTLENYEVAIQREGVPLQALTIYNADGVEVDTIYGTDTTS
                     FGFAGTEGNRLISIASHLSDLQEYAGKKIPLLYKPDAEPWQTTDVLRGKILLIDEVFG
                     ESDPLNNGIGAALQQNVDLSATAFTVSADLTSELKNILTTHHDLSASVILGEAISGVI
                     QGKEHPEKEIWIITHYGFSSNSLGADQSASGIATMLELARMFKNDPPAYSVRFIFLPA
                     QGGIYSGLIAYLQLHASEASHVMAAFDIAWTGNWEKILVSEYLDEMGTYVDATRLEDE
                     GQYFVQTYWAKSVDLDDDDMLKNFTQQVTRRMGLSESPAALQSAVEQAGEMLDIPLTR
                     SLDEGYFPLSLSILLSDDLPTIGIYGAGNDMLGSEYDDLSTIQEKDLVRATALIYQSV
                     QNVMGVTK"
     gene            complement(32182..32568)
                     /locus_tag="PLL88_00145"
     CDS             complement(32182..32568)
                     /locus_tag="PLL88_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOI99998.1"
                     /translation="MKKTAVCLLVCTLLFLALGVQPVYADQPELPQPLVIPAEINADP
                     YSQTVFSPNYQGVWEVGVFGEGTNYCIQACWGDPCGCASSCGYTTGTYYYYHQFRCGG
                     SSPFYQTWTLYNGPGAPVYDSSVVYK"
     gene            32927..33259
                     /locus_tag="PLL88_00150"
     CDS             32927..33259
                     /locus_tag="PLL88_00150"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOI99999.1"
                     /translation="MAAKFELKESKGQFIFNLKAPNGRIILTSERYTSKSGAMNGIES
                     VRKNGKDDAQFERLESKRGEPYFVLKARNNEPIGHSEMYSSKSAMENGIASVKENAPI
                     AEIEDLTE"
     gene            33415..34053
                     /locus_tag="PLL88_00155"
     CDS             33415..34053
                     /locus_tag="PLL88_00155"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HOJ00001.1"
                     /translation="MAIFVDSAILEEVQQAASWGWIAGATTNPSLLAKSPLPPRETLQ
                     RMAEILDGYIFYQLTSPTLEGMQHEALEAHRILQDQLILKIPATTIGFQAAARLSDDY
                     TCAITAIFSPEQALLAQACGARYAIYYHNRAKQWMPDGAELGPKMVRLLSGCDGIEVL
                     AASFKNKEEVFEVAGAGVKHLTIKLDLLKTLADNEFSQKAVDDFNATGCGIV"
     gene            34096..35109
                     /gene="galE"
                     /locus_tag="PLL88_00160"
     CDS             34096..35109
                     /gene="galE"
                     /locus_tag="PLL88_00160"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOJ00002.1"
                     /translation="MKFLITGGAGYIGSTICSALEDNGHTPIILDSLVTGCPEFTKGR
                     IFYKGDIADAALVQGIFEDHADIQATIHCAALIVVPESTQKPYEYYRENVEKSLELFH
                     SLYLAGCRRVVFSSSAAIYDTVPGFMVTEDSPLNPTNPYARTKMVMEMVLKDFCQAYG
                     IRGIALRYFNPIGADPKMRSGLQLQSPTHVLAKMVEVQLGRQSEFLITGTDYPTRDGT
                     GIRDYIHIWDLARAHVLAVTDFDGVFERAGSSSGNYLVINLGSGKGVTVRELVSAFES
                     VTGQPIKKRETARRPGDSAGSFANADLAARLLNWKTELTIEQGIADALKWGAIRDQVL
                     HYS"
     gene            complement(35106..36146)
                     /gene="holA"
                     /locus_tag="PLL88_00165"
     CDS             complement(35106..36146)
                     /gene="holA"
                     /locus_tag="PLL88_00165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOJ00003.1"
                     /translation="MQELERYEIKKGCSVYFFIGDDLDALQIVKQAMIRDVGDADMAA
                     LNVARLNGRTDSLDELIKNIGLFPFGVEKRLVIYSHSLERVRDKKEIERWQQIVENVP
                     PSTILVLEFPTNLIKKGRDWMWKPFAEHAWLSQWASRKDDGYFFKEFRVPARRDFPAR
                     IMRMAEEEGGRIEARAAEGLARMTGDDILIIRQEIQKLCTYVNGKRNITLEDVHVLCS
                     AIPEEDVFAMVDAFALGDAPKALHHLKLMFANQDYVRVFSMIVRQFRLLLLVKESLQE
                     NPAGNVASQLGESEFVIQKLIPQSKRFTFEELERIYRALYDMDGEVKRGSTTPEVGLE
                     MIFFEHIKRAAR"
     gene            complement(36151..36669)
                     /locus_tag="PLL88_00170"
     CDS             complement(36151..36669)
                     /locus_tag="PLL88_00170"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HOJ00004.1"
                     /translation="MELILEANPVWKIRSNHALEHATLHVLEAEGVRARVSGLSDSGG
                     FWLLGDVPSGMVLESAHEALQRMQAGETGLAMHDHCGSNLVPSIAVSSGLAWLAMAGA
                     GKNLRKKAMRIPLAVLLAWLGFEAARPAGPFLQEKAVSTDHMQTMLVREVRCYQRYGL
                     VIHRVTTEFKAD"
     gene            complement(36687..38081)
                     /gene="radA"
                     /locus_tag="PLL88_00175"
     CDS             complement(36687..38081)
                     /gene="radA"
                     /locus_tag="PLL88_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOJ00005.1"
                     /translation="MTKTETRYVCQSCGRVSVKPLGRCPQCGEWDSMVEEVVQPSGGG
                     KKRSAAGITTSRVLRLDEISGESAERWKMDNEEFSRVLGGGIVPGSIVLVGGDPGIGK
                     STLLLQTAIEFAQKGNVLYVSGEESGQQIKMRAQRLQAKGNYGKTTTFPPNLYLLMET
                     DLDSILTHAQNVKPQLLIVDSIQTMTMEVFNSSAGSVTQVRECASRLREFAKSSATTV
                     FLIGHVTKEGMLAGPRVLEHIVDTVLQLEGDRFQSYRILRSMKNRFGPTSEVGVFEMR
                     EAGMVEVSNPSEVFLAERMENTPGSAVAVTMEGTRPLLIEIQGLTSTTNFGNPRRTSN
                     GVDFNRLLLVVAVLTRRNHLKLFDQDVFVNVVGGFNVDEPAADLAIATAIASSLLDKA
                     VHPEMVLIGEVGLSGELRWVSNMVARLREAEKMGFTKAIIPQRLRKSEKLPTGIELIE
                     NRSLTDAVKSALNQ"
     gene            complement(38156..40657)
                     /locus_tag="PLL88_00180"
     CDS             complement(38156..40657)
                     /locus_tag="PLL88_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOJ00006.1"
                     /translation="MSGLEKFTQSSRQVLSLAHQEAEQFHHPQIHPEHILLGLIQENN
                     GVAGRVLRELGIELERAREMVLRLTGMGTINKERIDLSVDAQKVVALSLEEAQKMSSR
                     YISTEHLLLAIVNSNDALCENVLSKLGVTFEQVRRQITREINQKQAPLSKVSEVPEET
                     PTSSRRPRKTPLVDQLAVDLTTQAEEHKLDPVIGREKEIERVIQILARRTKNNPALIG
                     EPGVGKTAIVEGLAQRIVEGNVPIPLLGKRVLRLDVGSLVAGTMYRGQFEERLKRVID
                     ELKRSDAILFIDEVHMLVGAGSAGSSVDAANILKPALSRGELQVVGATTLDEYRKNIE
                     SDAALERRFQPVVVNEPSKEETLEILRGVRKNYEEHHHLVISDEALEAATNLSARYIT
                     DRFLPDKAIDLIDEAASRVRMYKSDAAKVTKQITDELRQVRTRHSEMLEAGDEELALE
                     VAGKIKDLEEKLNTIRDQWDRDSSPVVRTDDIAELVSMWTGVPIMQLAQEETQRLLNL
                     EKELHEMIVGQDEAIEKIAKAVRRGRAGLKDPKRPIGSFMFLGPTGVGKTELTKALAR
                     IMFGSEEALIQLDMSEFMEKHTVSRLMGSPPGYVGYEDAGQLTEAIRRRPYSIVVFDE
                     VEKAHPEVHNTLLQILEEGQLTDAHGRKVNFRNAIIVMTSNIGAEMIKRQSGLGFVLE
                     QDTKKEEANVYKEMREKLLDSLKKAFRPEFLNRLDNVIVFHPLNREHIRQIAEMELVK
                     VAKRLSEREIRLRASPAAVDKLAEKGYDPDMGARPIRRVIQLEIEDKLSDALLAETFR
                     DGDDILLDVDQEGDFILLRDDERIPDRGEILKAGA"
     gene            complement(40804..41220)
                     /locus_tag="PLL88_00185"
     CDS             complement(40804..41220)
                     /locus_tag="PLL88_00185"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOJ00007.1"
                     /translation="MSDFRFFYPVQIRYADLDAQWHVNNARFLTILEQARLAYVRQLG
                     LWDGKSFLDLGLIVADVHIAYKAPIELEDEIQVALKVSRIGKKSMSMENEIRGRKDGS
                     LKALAEIIMVTYDFHSKTTIPVPENWRKKITEFEGL"
     gene            41320..41736
                     /locus_tag="PLL88_00190"
     CDS             41320..41736
                     /locus_tag="PLL88_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOJ00008.1"
                     /translation="MQITRQADYALRTIIYLSRLGEDEKASTSNIAEKQHIPSSFLAK
                     IISQLSVAGIIHTSRGAHGGVSLAKSPDKISVLDVIEAIDGPVMLNECTHNPGICIFT
                     KDCPMHVMWCDTRKELVNKLETITFEQFSSSKYAVL"
     gene            complement(41760..43448)
                     /locus_tag="PLL88_00195"
     CDS             complement(41760..43448)
                     /locus_tag="PLL88_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00009.1"
                     /translation="MKRKTIEIALSVLFFIALWVGLSFNTGGPIYSDELLYIEIGLNN
                     ERAPNYGNRYFHVYLQKVFMAVAPTPLIGVRIYWAFVIALTALLIYWSTRIFLKESTA
                     MHGLLAVALFLSYKFISSYAGVTSVDITAMMMATALVFTYILYQRTEKAWLLYALGAL
                     TFLALKTKETTLFSNVILLGFFFDRQGKFSLKNVSPYLKNYFLGLLGAVGFFMILDAI
                     FLGQPFFAISPDTLKEVFENYAYTGGFRVEPANWYRIYLLDDLMIPFLLFLVGGVKLS
                     TRAITPQKKIVWAFPLLLVSFITLNMLKIPWGFIERFYFPALPAIAILAPQFISFDTP
                     HDRQGYLKFMAVILTAFLLVVVMRQLGMQYVEKIDWNYGKFLESIYFPILLSVFLGLV
                     ILVEKQNLLTVGILLFFLVSWLLPQISYNYKYIYHEPTTNAKFQIKYYPFLEFQDEIV
                     VSDDVKMYTTTTIFEETLENEDHMFSNNPYDILGMYNILFNQRIQRDNLTLSYTQEDI
                     PEQILADRYDFIILSVDDWQYLEEGFPEVLADLEQNYRIQFDENQIMVFMKPDS"
     gene            43566..44819
                     /gene="hutI"
                     /locus_tag="PLL88_00200"
     CDS             43566..44819
                     /gene="hutI"
                     /locus_tag="PLL88_00200"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOJ00010.1"
                     /translation="MLIHSASQLICLRGGPQRGEQLGSLDLIEDGAVLMHGETIREVG
                     STKDLLQRFPQEEQFDALHRVVMPGFVDPHTHLVWAGDRALEFSLRLEGKSYLEIMAA
                     GGGILSTVKATRQASTSQIEREAIRRARSMLHHGTTTLEAKSGYALDLHGELRLLEII
                     MNLRKSLPLDIAPTYLGAHAVPEEFKGNVAGYTELLVKEALPQLKEWWLLHYPDEKLP
                     FVDVFCEPSVFNLEHSRAILATARELGFPLKIHADEFENIGGASLATELGATSADHLV
                     KTSPEDIQKLASSNTVAVALPCTPFGLAESSYTPARSIIAQGGLLALASDLNPGTAWC
                     GNMQFVIALACRFMKLTPAQALAAATINAAAAIKLEHRVGSLEPGKQADLLVLNIPDY
                     RHLGYRFGENLVATVIKKGKVVDFS"
     gene            44816..45463
                     /locus_tag="PLL88_00205"
     CDS             44816..45463
                     /locus_tag="PLL88_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ00011.1"
                     /translation="MSHRALLDLPAVRDLYPQIDPVHGFDHIERVYRLCQVIGEAEGA
                     DMEILLTAALLHDCEGSDPTSQSRAEHHLLSADKAGAILRELRWAEDDIHKVQHCIRA
                     HRFRKSEEPHSIEAQALFDADKLDVIGAIGIARTIGYAVQAGEPIYSRPSEQFLATGA
                     LAEGEHHSVFHEYLFKLRNVHTTLYTETGCKLALGRHAFLDMFFQQLEEEVNGTR"
     gene            45494..46279
                     /locus_tag="PLL88_00210"
     CDS             45494..46279
                     /locus_tag="PLL88_00210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOJ00012.1"
                     /translation="MVPDLNYLMRIARQAGQILMEGYLKQHEIRHKGRIDLVTEMDSR
                     SEEYLLSEIRRDFKDHTIVTEESGLIEGVQEHCWFIDPLDGTSNYAHGVPVFSVTIAY
                     AFHGAITLGVTVDPTRNHCFSAERGQSAFLNGQPLHVSATRELVDAMLATGFPADLHD
                     PAKNNLENFSRFLMSAQSIRRLGSAALDIAYVAAGWLDGYWEIGIHPWDIAAGTLLIQ
                     EAAGLVTDVQGEADFFKPPYSLIAANPHLHAKMLAMLQMADTR"
     gene            46335..48110
                     /locus_tag="PLL88_00215"
     CDS             46335..48110
                     /locus_tag="PLL88_00215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOJ00013.1"
                     /translation="MTTQNDVFKQSRWSLNDLFPSANSPEMNNAFKQAEKMVADFENN
                     REILSEDIPADTFLQIVKDLEALTRQMRLINDYAELWFTEDTQNQQAQSLAARAEQFV
                     TEQSNKILFFTLWWKDLSEKNALRLMDVSGDYRYWLEEMRHFKPYTLSEAEEKIINTK
                     DLTGFAALIRLYDSITNRYTFRVAVDGEEKELTRGELMSLVRLPDANLRAAAYQQQYQ
                     KYGEEGPVLGQIYQTIVRDWYNENIGLRKYKTPISARNLGNDIPDDVVDTLLDVCKKN
                     TSVFQRFFRLKAKWLGVDTIRRYDVYAPVAESNKKYPFEMATELVLDSFQEFDPRFAQ
                     LAQRVFAQAHIDSEVRKGKRFGAFCATVAPDLTPWVLINYQGKAEDMATLAHELGHAI
                     HSLLASDHSIFTQHACLPLAETASTFGEMMLIDRLLAEEKDEGVRRFFLFKQVDDSYA
                     TIMRQAYFALFEKTAHEMTLQGAAVDAIADAYFENLQDQFGDSLILGEEFRWEWVSIP
                     HIYHTPFYVYAYAFGQLLVLSLYNQFKNEGNSFKPKYIHLLSTGGSKAPMQILDEAGM
                     DVRKPEFWQGGFDLIATMVAQLETL"
     gene            complement(48155..48538)
                     /gene="ssb"
                     /locus_tag="PLL88_00220"
     CDS             complement(48155..48538)
                     /gene="ssb"
                     /locus_tag="PLL88_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ00014.1"
                     /translation="MASLNRVQLIGRLGKDPESRKTPKGTEYTTFSLAVDRKWKDKKD
                     ELHQEADWFNIEAWGKLGEICQKYLEKGKLVYLEGRLRTRKYEHEGTTRYFTTVVLGA
                     IQMLDRKAKETVLEEVEESEEEVND"
     gene            48624..49265
                     /locus_tag="PLL88_00225"
     CDS             48624..49265
                     /locus_tag="PLL88_00225"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOJ00015.1"
                     /translation="MYIAIEGVIGVGKTSLARLLQPSYKSNLLLEIFEENPFLQEFYQ
                     DRARYAFQTQIFFLLSRYHQQHNNVPELLQSQKNLISDYTFEKDALFAGVNLKGDELD
                     VYHSVHTALAERIPAPDLTVYLKATTDTLMQRITMRDRSYERSMEPDYIATLNEAYER
                     HFTSAGKEGSPVLVIDSNDLDFVHNPADLQFIDDRIRQTLRLPPFQVALPMDG"
     gene            49284..50291
                     /locus_tag="PLL88_00230"
     CDS             49284..50291
                     /locus_tag="PLL88_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00016.1"
                     /translation="MRVTRNMLLETARNFVKEKTFNDPDVACVFLVGSLVGEDPFLGE
                     STDIDLIFIHNTEPALSREVRSLGDDFQLDIFHYPRRVFNQPKELRQDPWLGCLFCET
                     PFVLYDTYHYYDYLRAGIFSNFFSPVNSYARSQFFYTNARNRWMNLQHTAEEFSADVV
                     YDYLLTLYDAGNCISCLTGRPLAERRFLSELEFCTNYLGRPGLASGMRDLYGINDYSA
                     FPWETFLEQMKNAILALSEKSYCPPQFSSVRFRYYQQAISHYQAERPDEALWILFWVW
                     TSVIHTLPKRASEAKVWKNFCEQLDFTRDIFVQRLQNLDSYLEAVGTTCTEWQRVSGL
                     L"
     gene            50792..51052
                     /locus_tag="PLL88_00235"
     CDS             50792..51052
                     /locus_tag="PLL88_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HOJ00017.1"
                     /translation="MDVIKVKAVSRTAAVAGAIAGVIREHKHAEVQAIGAGAVNQAIK
                     ALILAKNFLDQDGITVVFIPEFVNVEIDGKVRTAIKFIIEPR"
     gene            complement(51109..51765)
                     /locus_tag="PLL88_00240"
     CDS             complement(51109..51765)
                     /locus_tag="PLL88_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOJ00018.1"
                     /translation="MKKFITIAGNIGAGKSSLVCLLSQRLDWNPFFEPEANNPYLSDF
                     YADMYTWSFHSQIYFLSNRLKVYQQLLDCPNSVILDRSLYEDAEIFARNLYLQGFMNQ
                     RDHETYETIYQTLLHFLPIPDLVVYLRASVNTLEQRIRRRNRNYEKNISTDYLQTLND
                     AYETWIQNFTLCPVLTVPSDNLDFVNHTRHMDLILEKIQEKIAGKEEVVFLPEEYNQY
                     "
     gene            complement(51847..52020)
                     /locus_tag="PLL88_00245"
     CDS             complement(51847..52020)
                     /locus_tag="PLL88_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010266056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ00019.1"
                     /translation="MTHVITDECIVCGSCEAECPQDAISMGEEHFEIDPAKCIDDGAC
                     VEVCPVDAIIKES"
     gene            complement(52133..54355)
                     /locus_tag="PLL88_00250"
     CDS             complement(52133..54355)
                     /locus_tag="PLL88_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOJ00020.1"
                     /translation="MSKRKLKRQLNLLQIIMLGTAGTIAAEIFVLTGHAAGIAGPDAV
                     LALLIGGLLTLSVALNYCELATTYPVTGGAMTYVGEGFGKGLIMFLVGSLDGLSSAFY
                     AALSSIGFAYSVSVFIPNLPIIPVALGVIILVGIMHVRGVKQAGNLQIVLGGFLLVVF
                     AIYIVRGLTHPNGFNVDTFQSGRVLFENQSLAANIGRMLTTIALVYNAYVGLEVIADD
                     AEEVMHPQKNIPLGILISLGITTVVYTLVSLVTIGTIPYTTLAGSETALTDAASLFWP
                     KVGVPVMGLAGIVATFTAANSAMLSATREAFTLSRDGVWPRIFSRLSHWRTPYVAIIF
                     IMIVSGLVALLQVVDLLSYISSAGYMFVLFWATLAMFRLRKMHPDIKRPFKVPFFPLT
                     ACLAAASCVLIITFASKAALQFLGLVLFILSVIYYIAKWINKLVEKHEKVEEQVGGGR
                     ILVPTVNPKTAVGLVKLASRLAEHQEDTSICLFNVVKAPRTASQEIIQDLIEKTKKIE
                     SDLLNATAPIAQARNVPIYTKHKLAPNVETGIFNELKSPNPVQMVLMGWPSTKSKIMM
                     PHNIIKEVLVMAHEKVGVLRDSGMNGLENILVPVGNGPNAHLAIKIASDLAFHHDTKI
                     TALRVIPKHANQETQEDEMLQAQEIIENELGEVPPYLETRVVTSNDVLEGIVSEAEKK
                     SYDLMIIGASEEVFSPHYIFGKLNDALAEEVACSILIVRRYQPGTALWFRQRIKEIEE
                     "
     gene            54579..54651
                     /locus_tag="PLL88_00255"
     tRNA            54579..54651
                     /locus_tag="PLL88_00255"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54612..54614,aa:Asn,seq:gtt)
     gene            54749..55597
                     /locus_tag="PLL88_00260"
     CDS             54749..55597
                     /locus_tag="PLL88_00260"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ00021.1"
                     /translation="MKHWKIATLLVFSLVFLQACDVAISVGDADGSKASVPLAVNVQA
                     PAQGSQVPMGAVDIRYTATAPEGVAVVELSIDGYIVNSYTAPDPSQNTVALVYSWTPP
                     TVGSHIIRVRVQDSKGTWSDYTDVMLTVTEAQPDQSAADQSSQQAQPTDAPLPTATPS
                     TPYISGVTHDVNKFYYGNSTCGPTKITISAKVSDPSKVWSVVIFTRFMDKEGEGQSKW
                     DTGHAMNPKGSSGEYSITLDSSKIANFNTYDYAVFRYQFVATDKNRNEVARTDVYEDI
                     DYSICP"
     gene            55778..56332
                     /locus_tag="PLL88_00265"
     CDS             55778..56332
                     /locus_tag="PLL88_00265"
                     /inference="COORDINATES: protein motif:HMM:NF025343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ00022.1"
                     /translation="MQTAQELFSTAGYDATGVSQICAACGLSKGAFYHHFPSKHAVFM
                     HLLRTWLEQLDKEFFTVREETDDVLAGIATMAASSDVIFDAVRGKFPLFLEFWERSIR
                     DERVWEETKAPFTKYHLYFSQMIKHGVANGTIRPVEAASASRALIGLAIGIILQSLLY
                     PQDADWKAELQNSMDILLRGLQNG"
     gene            56325..57323
                     /locus_tag="PLL88_00270"
     CDS             56325..57323
                     /locus_tag="PLL88_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ00023.1"
                     /translation="MDKGKVLITGAAGHLGNVLVRELLEHGYEVRAMVMPNEDVSALV
                     GLDVEIKLGNVLDPASLDRVLKGIDYVFHMAGIVSIVPGEEELVWNVNVEGTRNVLKA
                     ARKAKVKRLVYTSSIHALSRQWEGTIDEHVPFDPDNPVGTYDRTKATASLAVNQAIKE
                     GLDAVIVCPTGVVGPYDFRGSSMGELVNDLTRHHVHVLVEGAYDFVDVRDVAAGHRLA
                     LEKGKTGQVYILSGHQIKLLELKEMVQKIVGFKTLTINVPFKLANIGADIATLYYKIA
                     KKRPKFTRYALETVQSNSVISNTKARRELGYSSRDVFMTIADTVKWWQTKKTRNNG"
     gene            57327..58130
                     /locus_tag="PLL88_00275"
     CDS             57327..58130
                     /locus_tag="PLL88_00275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ00024.1"
                     /translation="MRSKTALQGKTALITGASSGVGRQVALDLAARGVRVMLVARRED
                     RLVSIQREIENHGGEAIYHVCDLEDENARRGLISHLLKDTPVDILINNAGFGWYGYYA
                     DMPWEIARDLVQVNITAMLALTRALLPGMRDRGYGHIVNIGSIVGGFPNQGVAVYAAS
                     KAFLDAFSTALFREMKGSGVWVSALRLGPVRTEFFERAKNLPNGRKVPAETFSISVEK
                     AARGVLRLLQSPKRYLYVPAALSVTVIIGLLFGGLIDSLGPLLLRKSGD"
     gene            complement(58107..58424)
                     /locus_tag="PLL88_00280"
     CDS             complement(58107..58424)
                     /locus_tag="PLL88_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00025.1"
                     /translation="MSSKQSRFYFHPNFSRYPFANSRSFGANTYTVLRQRGEQQLFRP
                     PSLSNVSSLWVMEKIMLDHGNRVQLVDVFRRSERLKANTPAFQAILKRAPDRTASVPA
                     LSQ"
     gene            58572..59834
                     /locus_tag="PLL88_00285"
     CDS             58572..59834
                     /locus_tag="PLL88_00285"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOJ00026.1"
                     /translation="MQTLDNTMAVIMGGGQGTRLFPLTQQRAKPAVPFAGKYRLIDVP
                     ISNCINSGIYKIAVLTQFNSVSLHRHISNTYNFDAFHGGYVQIWAAEQTGESTAWYQG
                     TADAVRKQLFEIKTTGVPFVLILAGDHLYRMNYADMATFHWKKGADVTVAVQPISRED
                     APRCGVLKRDKEDRIVRFTEKPQEEKLLTEMVSQPGSDKPYFGSMGIYFFKTEVLCKL
                     LEQTDYDDFGKHAIPAAVQEYQVYGYSYDGFWADIGTIRAFYETNLALTEEKPPFSLI
                     DEGWPIYTHARFLPGSQIENSHIDHTLLAEGCWIKDASISHSVIGLRSQIRAGVTIKD
                     SIIMGADYYGFFRSEVSELKSIPLGIGNDCYIEGAIIDKNTHIGKGSAILPFPRGTEL
                     DTAHWMVRDGIVVIPKNTVLSPGTRIAP"
     gene            59901..62741
                     /locus_tag="PLL88_00290"
     CDS             59901..62741
                     /locus_tag="PLL88_00290"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOJ00027.1"
                     /translation="MTAKNTADVQRAADRDIEIIWHSLSQEEVLKKLHSPIEKGLSSQ
                     EAAARQEKYGLNELQEGKKRTFLKMVFDQLNNFVIIMLVIAAIVSAFLGEIIDASAII
                     AIVILNTVMGVVQESRAEQALDALKKMAAPEAQVLRDGHRISVPARDLVPGDIVFLEA
                     GNFVPADLRLLEAVNLKIDEAALTGESVPVEKNAALLLDSNASLGDRINTAFSGTVVS
                     YGRGKGIVVSTGMNTQIGLIATMLQSVEEEQTPLQIKLDQLGKLLGYACLVISAIVFV
                     AGWLQGGDLLESFMVAVSLAIAAVPEGLPAVVTISLALGMHEMVKRHALIRRLASVET
                     LGSATVICSDKTGTLTQNVMTVTEVWTDGKTVNITGTGYVPEGKFVFDGKTIELNEVP
                     AVATTLWVGSVNNDSELEVIEDENGKMNYRIVGDPTEGSILVAAAKAGVHAADLKRAY
                     PREGEIPFDSTRKRMVTVHGIKETWKASNLPFNGKGKESAYVIAVKGAPDVVLKLCNR
                     YQKADNTTVSLDDAARRRILQANDGMTQKALRVLGMAYRVVSAKPQDVDSEHLEKDLV
                     FAGLVGMIDPAREEVKPALMKAIGAGIRTIMITGDYPNTAKAIAESIGLLRPEHRVVT
                     GADIESLSDEQLQKEVKTIDVFARVSPEHKMRIVNALRANDETVAMTGDGVNDAPAIK
                     TADIGVAMGITGTDVAKQTADMVLTDDNYASIVSAVEQGRIIYSNIRKFVYYLISCNI
                     AEIMIIFLPTVIGKFLFPFAAASLAVSSPLKPIQLLWLNLVTDGAPALALGTEKGDPD
                     IMDQSPRPKKEPIINRFMLVGIAIQTIFIAGATLLAYSFGLRMGNQQTAETMAFVTLS
                     LSELLRAYTARSERYPLLKMGVFKNKWMNIAVLASVLLVMAVIYIPFFNVIFDTVPLT
                     PVEWEKIIPLLLLPSVAAEVVKYVVSRKKKTA"
     gene            complement(62793..63443)
                     /locus_tag="PLL88_00295"
     CDS             complement(62793..63443)
                     /locus_tag="PLL88_00295"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOJ00028.1"
                     /translation="MARSRTPPAHRPTLEHELQLWQQGYHSIAGLDEAGRGAWAGPVF
                     AAAVILPHDERILQLLNGVNDSKKMTPHQRERWQGCIKAASLDWAIGSVSNQEIDQMG
                     IVAATRAAMLRALAQLRYKADHLLIDYMRLTESNLPQICLPKGDSLCLSIAAASILAK
                     TARDALMIEIDSQYPTYGFAAHKGYGTARHRTALQTTGLCEIHRVSFEPMRSVHGN"
     gene            complement(63433..64158)
                     /locus_tag="PLL88_00300"
     CDS             complement(63433..64158)
                     /locus_tag="PLL88_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ00029.1"
                     /translation="MNKPFLSIVIPAYNEEARLPASLAAIATFLSQQSYRAEVLVVEN
                     GSSDHTFTIAREFAQRYPTFRAIHIAERGKGIAVKTGMLAAQGEYRFICDADLSMPIE
                     EVNNFIPPALKGCDIAIGSREAPGAQRIGEPAYRHFIGRAFNGLVKVMALPGLQDTQC
                     GFKCFSARAADQLFPLQTIQGWTFDVEVLFLARRFGYAIHEVPIHWHFNAHSKVRFLR
                     DSLHMAADLIRIRWKAFRKEYGS"
     gene            64337..64915
                     /locus_tag="PLL88_00305"
     CDS             64337..64915
                     /locus_tag="PLL88_00305"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ00030.1"
                     /translation="MSPKEPRVWETVSKKTILKHSKFLTVENHIIKLPGGQVISDWPW
                     IIIPSAAIVLAVTENDEFLCFRQTKYAVEGTTLAPVGGMLELDEKPMDAAKRELLEET
                     GYISAHWVSFGSYILDPNRGIATMNLFLALKAKQVTEPNSDDLEDQELLLLSQNEIED
                     ALKSGEFKILAWSTVVSMSLNYITNSRNHGML"
     gene            complement(64981..65053)
                     /locus_tag="PLL88_00310"
     tRNA            complement(64981..65053)
                     /locus_tag="PLL88_00310"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65018..65020),aa:Pro,seq:tgg)
     gene            65108..66019
                     /locus_tag="PLL88_00315"
     CDS             65108..66019
                     /locus_tag="PLL88_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOJ00031.1"
                     /translation="MRSQPRSQEIIRMFLLLTSASLLVGCQPAATVPLQSTLSPTATL
                     LPSPTPNVQLTSSSAQTTEAQPLCEESGSIRSYTLASELLGEALDFDVYLPPCYDSAS
                     GQTYPVIYLLHGQWQNTALWQTIGIQPTADELILSGQRQPFLIVMPYEKYYFRPAENN
                     HYPDALVQELLPWVEDNLAACGQKECRAIGGISRGAAWATRLALQNPDMFSALGAHSM
                     SPFNGDIEELPDWVATIPQDELPRIYLDVGASDPTVKDALAFEQTLNTLGVAHEWHLN
                     SGRHNETYWSEQIANYLSWYTLNWMEE"
     gene            66030..66914
                     /locus_tag="PLL88_00320"
     CDS             66030..66914
                     /locus_tag="PLL88_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOJ00032.1"
                     /translation="MILEKKLQWLVLITMLGGILAACSTHGAADTVPTATAALTPTEE
                     LPTATPSPQVCTETQGYFEYQEIQTALMTHPLSFRVYLPVCYDPDGEYEYPVLYFLHG
                     QSFNDDQWDRLGADEALDRLILAREVAPFIIVMPKESDYMSNQWDSKYGPAIAEELTV
                     WVDEHYRTCAERECRAIGGLSRGAAWAMRTGLIYWQTFGSIGTHSLAPFRGDFNEAPF
                     WFKEIPEDQLPRIWIDIGVLDVNLDAANTFEVRLTKYRIPHEWHIFLGTHNEDYWQAH
                     VEEYLRWYAEAWKEKMDQ"
     gene            66911..68914
                     /gene="ccsA"
                     /locus_tag="PLL88_00325"
     CDS             66911..68914
                     /gene="ccsA"
                     /locus_tag="PLL88_00325"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HOJ00033.1"
                     /translation="MIANIGYLLLVLGVVCSVLAVLICLWALIKKVRSFVMETRVLSA
                     IGTLAVVAAFALAIVLLVSGRFDYEIVYENTSRQMDLLQKVTAVWGNKSGSLLFWSFL
                     LSICTSVVAFGKWQDKKEPYFHGVMLLLQLINLFFVALITFQTNPFARLWQLPNGNVA
                     AAVFAPASALPFNPGDGQGLNPLLKHLGMVIHPPLLYLGFILFFVPFAFALVSLWRRD
                     YSFTWLNSSRTWIVLAWVFLTAGILLGCWWAYDILGWGGYWSWDPVEIAGLMPWLSGL
                     GLLHCFNTYRASAHVRRWAYVWIVLTVLLIIFGIYLSRTGVVTSVHAYSQSSIGPAFM
                     GFFIFLFVLSIGTLVARWKDLRPEAERPSYLSQAGLACTLSLCMALLVLVCLVGVTLP
                     LTSQALTGSRTEPSQLYYEWSFAPPLLVVLISIALGYLPQNKSYSTLSGILALVFTIG
                     VGLSLGKMNFSALVGLWVVFFLISALLFAAADRLRRSSSSVTPKKRFPRLAGIVLHLG
                     FALLALGVVGSRNLADSNVVSLQAGQSTALDGMQVTKTSGEVEVAANQQVLYAEEYVF
                     QQDNRRAVVLHPHILWYDDTRSTVSSPAIHSTLLTDTYAAILEWDGFANGTTTVLLST
                     FPLMAWLWIGGVLLMLGTVWLHVANRFSRKDDSESEVPDRTAK"
     gene            68997..69443
                     /locus_tag="PLL88_00330"
     CDS             68997..69443
                     /locus_tag="PLL88_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00034.1"
                     /translation="MEKGWKIALIVVGAFLLICAMVFLVSYFAGGRFYRGYDSYGSVR
                     RWDDSADRYFRMPHGGMMRRWDGSSAPNEDFYLEHWIAILGISKEELQNRLDSGESLE
                     SIAESLSIEMPCWDRNVDDEHADLSTTPTRDDGWVNGAGMCFQEYP"
     gene            69499..70179
                     /locus_tag="PLL88_00335"
     CDS             69499..70179
                     /locus_tag="PLL88_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ00035.1"
                     /translation="MQKILIIEDELELVKVLRSYLENAGFTVISALRGDSGLVAWQNE
                     RPDLVLLDLNLPGMDGIELAKSIRKTDATPIVMVTARVEDIDRIVGLELGADDYISKP
                     FSPREVVARVKAVLRRTQPTEGSVKKLIIKDLEIDADAFKVKQAGSTLDLTRTEFAIL
                     QLMAEHPGKVFTRLQLLEATQGVAYEGYERTIDAHIKNLRSKLGDKADQPKYIKTSFG
                     IGYSFREN"
     gene            70183..71556
                     /locus_tag="PLL88_00340"
     CDS             70183..71556
                     /locus_tag="PLL88_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ00036.1"
                     /translation="MGMRWRIFLSFLLIILVAVGSLAFFAYRSTTSEIASFVTRGGLW
                     GIEEMVKALEEYYQEHQTWQGAETILASSQTGKGMMQHSGMMMGGMHENAFRLVDADG
                     YVVIDQRNPQSEGSFLDTNPPGAVVLTLDETVIGYLLPESGYMFPTVDINAALLELLL
                     PSSIRAAVISGLVGLGMAVILGYFLYKPIRALEKAAANLAKGDLTQRVGETGPAETAN
                     LGKTFNRMAGELEKADRTRRNMTADIAHELRTPLAVQQANLEAMLDGVYPLDKKNLQV
                     VLDQNHTLRLLVDDLRLLSLADAGELGLLQKPIELTGYLRAFLEKFKPRLQEKRLSLY
                     VALPDSEIGMITDALRLEQILTNLMQNAVTHSPRRGKLYFAVAQSEDEVSITLRDTGK
                     GINPELLPVLFERFSKGSSKDRDRESTGLGLAVSKKLAQALGGALTAHNYPTGGAEFT
                     LTFFIKK"
     gene            71598..71885
                     /gene="gatC"
                     /locus_tag="PLL88_00345"
     CDS             71598..71885
                     /gene="gatC"
                     /locus_tag="PLL88_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOJ00037.1"
                     /translation="MSLSRKEVEHIALLARLDLSEEEIQRYAEQLSAILEHVAALQEL
                     ETGHIPPTSSVLPLRSGLREDEPQPGLDTGRALQNAPQAEKDQFRVPPVLD"
     gene            71892..73373
                     /gene="gatA"
                     /locus_tag="PLL88_00350"
     CDS             71892..73373
                     /gene="gatA"
                     /locus_tag="PLL88_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOJ00038.1"
                     /translation="MADFRKYSIQELMTGLRAGDFSSREVTSYFLDRIETLEGQVHAF
                     LSLKPEQALQQAERADAVYVAWRAEGRQSSLPALLGLPIAVKDVLCVDGFPCTCGSRI
                     LQDFHPPFNATSVQHLLDAGVVIVGKTNMDEFAMGSSTENSAFGPTRNPWNLAHVPGG
                     SSGGSAAAVAAHMIPAALATDTGGSVRQPASFCGVTGVKPTYGRVSRFGLIAYGSSLD
                     TAGVMAHNAQDVEALLRIMAGFDPRDATTSDLPVFDPAPRESDLKGLRIGLPLEYFIE
                     GIQPEVEMAVKEAVRLLEEHGAQVVQVSLPHTRYALPVYYLIAPAEASANLARFDGVR
                     YGLRVEDEDLIPMYSKTRGKGFGAEVKRRIMLGTYALSAGYYDAYYGQAQKVRTLIKQ
                     DFEKAFEIVDVIAAPIAPTTAFKLGQHTDDPLAMYLEDVFTLPANLAGVPGLAFPVGF
                     DGNSLPIGMQLMGPQFSEQVLFKTANVYQSFTDFHRRIPLSVQ"
     gene            73383..73520
                     /locus_tag="PLL88_00355"
     CDS             73383..73520
                     /locus_tag="PLL88_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00039.1"
                     /translation="MSYYGGADEVALPTDRLADGSSFTCNLTVRNDVVTQLSLLLNNK
                     L"
     gene            complement(73663..74493)
                     /locus_tag="PLL88_00360"
     CDS             complement(73663..74493)
                     /locus_tag="PLL88_00360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ00040.1"
                     /translation="MTDFEKIKAYYHVFNEWGRLDAPEGRLEFDLSMPIITSHLPPKA
                     EILDLGGGPGRYTIELAKSGHILHLADLSQTLLDEAKKKISEQGISNVKSITQVNAVD
                     LSRYNDCSFDAVLLFGPLYHLTNEEERVSCVREINRVLKPNGLVFASFIPYLTGAIGV
                     AGRMFYFPDQVSTETLKRVFDNGIFNNNANKGFQEGYYPTSHEVADLFEKNGFSKVLL
                     RSIRGWGSSREEQIYKLKDENPEKYVAVINLINETADEPAIIEMCMHAIYVGKSKTIR
                     "
     gene            74708..76411
                     /locus_tag="PLL88_00365"
     CDS             74708..76411
                     /locus_tag="PLL88_00365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOJ00041.1"
                     /translation="MQFKNPNTQTLLTWQWQNFEPYFTDLLALELNGSNITAWLTDWT
                     DVASAADELFNRLYVATSVNTVDEAAEQRLKDFMEKTYPPIMEKSQQLKEKLLASNLT
                     PPGFEIPLRNMRSEAALFRKENIPLEVELNQLSVELDKILGAQTVEWEGEERTARQME
                     VVLRDKDRAKRAAGWELSFKRQLTDRQAINENWVKMFEIRQQVAKNADKPSYREYIWE
                     SYKRFDYTPADCMRFHDAIEEVVVPAVNRQSEKRKKALGLATLRYYDLFVALDDYPPL
                     HPFSDAAELEETTSTIFHKVHSQFGEYFEIMRREGLLDLANRKNKADGAYTTDYAMIK
                     RPFIFGNAVGIHDDVQTLVHEGGHAFHAFETYKLPCIQQRWTPMEFNEVASMAMENLI
                     MPYLTKDQGGFYSERDAARAIISHLEDNLRFWPYMAIVDAFQHWAYENPKGGLDPDAC
                     DAKWSELEDRFRPHLDWSGFEDIKMTGWHRKHHIHQAPFYYIEYGLAQLGASQVWLNA
                     LKDQKYAVEKYHSALKLGGTATLPQLYEAASVKLAFDAATLKKVIDVMEDKITEMEAK
                     L"
     gene            76843..77415
                     /gene="xpt"
                     /locus_tag="PLL88_00370"
     CDS             76843..77415
                     /gene="xpt"
                     /locus_tag="PLL88_00370"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HOJ00042.1"
                     /translation="MQQLIDLIKKDGIYLGNGILKVDSFINHQVDPRIMEECGREFAR
                     LFASTHPTRILTAEISGIAPALMTAKFLDVPVVYARKHKPITMPDTVYLTVAPSHTKG
                     VMVDLIISPEYLQPGERVLIIDDFLASGATINGLVRLTQAAGATLVGIGALIEKEFEG
                     GREKLSELGVPIESLACITAMDENGIALKE"
     gene            77472..79022
                     /gene="guaA"
                     /locus_tag="PLL88_00375"
     CDS             77472..79022
                     /gene="guaA"
                     /locus_tag="PLL88_00375"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOJ00043.1"
                     /translation="MSIAILDYGSQYTQLIARRVRELQVYCELFPWDTPASEVLAIQP
                     QGVILSGGPASVYDPGAPTLPEYVLTGELPVLGICYGMQLLAYILGGKVSASSQHEYG
                     SAEISLIQKNRLIPEEIHRVWMSHGDRVDGMPSGFALLATSETCPVAAMGDETRRVYG
                     IQFHPEVAHTSQGIRILSNFLFDICDCRPNWTAQSIIADVVERVQKQVGSQRVLSAVS
                     GGVDSSVATALVHQAVGAQLEAVFVDTGLLRQGERESVQAALTPLLGEHLHVVDASTT
                     FLSALQNVNDPEQKRKIIGTTFIRVFEEQARRLGDLPFLVQGTIYPDVVESRAPERSK
                     AQRIKSHHNVGGLPEQLSFKLVEPLRYLFKDEVRAVGEALGLPPELIWRQPFPGPGLA
                     VRCVGAITAERLASLRAADAIFQNALKQAGLINQRLKDGETPIAQAFAALLPVRSVGV
                     MGDQRTYQETVVLRAVTSQDFMTADWARLPQDLLASVSNRIVNEVPGVNRVVYDITSK
                     PPATIEWE"
     gene            complement(79030..81216)
                     /locus_tag="PLL88_00380"
     CDS             complement(79030..81216)
                     /locus_tag="PLL88_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ00044.1"
                     /translation="MNDIFSDLNPQQKAAVSAPDGQTLVLAGPGSGKTRVLTQRAAFL
                     ILEREVDPFRILAVTFTNKAAREMQERLQRSLGGKADGLWLGTFHSICARILRREADA
                     LPFTRDFVIMDADDQQNLVKLSLKELNIDDKLYPPASLHAAISNAKNNLIQPKDFAAS
                     SYREEVIRRVFEQYEQKLQASNALDFDDLLLYAVRLLQENDAVREKYARRFYHVLVDE
                     FQDTNMAQYELLQLLASHYQNIFVVGDEDQSIYRWRGADYRNVLRFQKDYPNHTKLLL
                     EQNYRSTQNVLDAASAVIDRNSQRTPKKLFSKRGKGDKIVLYEAIDDHAEAMFAVETI
                     RALVESKKAKGGDIAIMYRTNAQSRLLEEAFFQSRMPYRLVGAQRFYGRQEVKDMIAY
                     LRTVQNPADEVSLLRVINLPPRGIGDKTIKDLRTCAEKAELSMGSVLLDLGSKQDASP
                     FWNAFSPRAASALAAFGALLANWRVLSPTILLPDLFEHILKDTNYQEFITDASSEVGY
                     SRWENVQELLRMAYEYSQVGLVEFLENLALVSDQDTLPEKSDSPTLLTLHAAKGLEFK
                     VVFIIGLDEGTLPHRRSMDDPEEMAEERRLFYVGITRTKDHLYLVRADQRRSFGSFDY
                     SVPSRFLDEIPENLLLRAGLQHYRSKSFSLQSVQPRWTSTSEKPKSAPKESAQYSAGM
                     HVKHAVLGNGIVIQSKLQGDDEVVTVAFDKAGLKKLSAAIAKLEIL"
     gene            complement(81307..82530)
                     /gene="pepT"
                     /locus_tag="PLL88_00385"
     CDS             complement(81307..82530)
                     /gene="pepT"
                     /locus_tag="PLL88_00385"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002448057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOJ00045.1"
                     /translation="MTLIERFLRYVRVNTRSNDEITDRTPSTEVQWDLARVLEEELKE
                     LGMQDVQLNEKCFVTATLPANTEKKTPVIGFLAHMDTSSDFNGENVNPQIIENYDGKD
                     IPLGSTRTLSSKEFPDLKKYIGQTIITTDGSSLLGADDKAGIAEIMSAMEYLLAHPEI
                     KHGTVKIAFTPDEEIGLGIRQFDVEQFGADFAYTLDGGPLGELEYENFNAARAYITMT
                     GKSVHPGDAKDVMINSQRVFFEFDSLLPEDQRPEHTEKREGFFHLWKMPAASVEQTQA
                     VYLLRDHDAVKFELKKQRILDTAEFLNKKYGAGTVKVEITEQYRNMREVLEPVMHIVD
                     TAKQAMRDSGITPIEQPIRGGTDGAQLSYMGLPTPNLFAGGHNFHGPYEYIVLESMQK
                     AVDVVVRIIELYAQK"
     gene            82561..83652
                     /locus_tag="PLL88_00390"
     CDS             82561..83652
                     /locus_tag="PLL88_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOJ00046.1"
                     /translation="MGILSSPEEKIKREDSFFVQAFAIHLCLRYNHGMANWEMIGQEW
                     AVSMLQQHVRSEQVRHAYLLCGPQGVGRRTLGLRFTQALNCPNPKEPGIPCGICRMCN
                     QIEAMQQADLHVVQAEIQGGILKVENIRDLLHDLSLTPYESKYRVALLLRFEEANEST
                     QNALLKTLEEPNPRVVILATAENTEKLLPTIVSRCEVLRLRPVAPDVLAATLRERDVE
                     AQDADLLAHVSGGRPGAALRMRTEPSLLEKRRQYLQDLLDLEQSPLRDRFKYADRITH
                     YKDGGREETKEELRAQLETWLSFWRDVLLTATNVQVPPGNPDYAEQARALASRLGVRR
                     AKAKVLAHQQALERLYFANLRLLMEIVLL"
     gene            83823..84503
                     /locus_tag="PLL88_00395"
     CDS             83823..84503
                     /locus_tag="PLL88_00395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ00047.1"
                     /translation="MMTNNLPTIRRKNYAQQARDIIETAIMRGDFALGEKLAEENIAR
                     QLNISRVPVREALRALEKYGLVEVKPGNGVWVISPDAKDIEEVFDIRALNEPYALELC
                     FKNSPEKTILELERQIADFESLLEQHGGRYDLINQDLEFDEIIFLQCGNNKLIELWSI
                     LAPIIKIGFFHNPYFKEKNSVVNNRSHREIVTAFKKGQIDTAKNLLIDHILKSKKLIC
                     ESFKKEEE"
     gene            84691..85377
                     /locus_tag="PLL88_00400"
     CDS             84691..85377
                     /locus_tag="PLL88_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOJ00048.1"
                     /translation="MKKTITQIYGISSVHDAKMVVDMGADHLGVAYGEVGKTPGFLDF
                     DQAREIFECIGDKAVKVGLTVAFDLEEIVEMVNTLMPDILHLSGDIAKFSPEQVKKLK
                     ERVPGLKVMQAIPANDPKVYEYINAYEAVSDYFLIDTVAEGIVGIGASGLTHSLEIDK
                     KIVESTKVPCIIAGGLGPENVAEAIRVVGPYGVDSLTKTNWDKSKGIRGKDPEKVKAF
                     IEAVREVERE"
     gene            85374..86333
                     /locus_tag="PLL88_00405"
     CDS             85374..86333
                     /locus_tag="PLL88_00405"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ00049.1"
                     /translation="MTEGVCVLGDTAVDLIVRLPEINNGVKVMAHAPNLNPGGSGANS
                     AVALARLGIPTQFIGTIGEDQYGQVVEQDFINEKVGVDQLFVEPTLNTVCVFAFIDQD
                     GERFLWGWPKENASFISIEFERINLDRIKETGWLHTTGILLGTESSGRDATLKALEWA
                     HKNGVTTSLDLNLRVGESGQALEKEFYDVVMNAVSNSKYVLGSDQEFSLLDPSNNWEK
                     TVEKLVKLEKVAVVRRGKEGSVLITDGQRISSPAFEVDVVDTIGAGDVFDAGFIAAIL
                     KNHDFSTALQWGNAVAGYTISMEGARTSPTFEQMEDFIRKKMG"
BASE COUNT        21617 a        21562 c        21345 g        21887 t
ORIGIN      
        1 ctggagctgg cgcgcattgc cgaagaacat gacctgatcg tcatctctga tgagatctat
       61 gaccggctgg tttatggggt cgagcatact tgctttccgg ctctccctgg tatgcaaaaa
      121 cgaaccatcc tggtgggagg gttttccaaa gactatgcca tgaccggttg gcgcctgggg
      181 tatattgctg caccgtctga actgatcaag ggcatgctgc tcattcacca gtacaccatc
      241 atgaccgccc ccaccatgtc gcaggtggca ggcatcaccg ccattctgga ttgtgagggg
      301 catgtggaag ccatgcgcaa ggaatacgac agtcgtcggc gattaattgt cgatggtttg
      361 aacaccattg gcttgcccac tgtggaaccg catggcgcat tctacgcctt ccccaaagtc
      421 aatgtgaccg ggctggatga atccaccttt gcaaaacgat tgctcaacga aaagaaggtg
      481 gctgttgtgc cagggatcgc ttttggaaaa gccggcgagg ggttcgtgcg ctgttcgtat
      541 gccaccgctg ctgaccagat cgaagaagcg ctggatcgta tcgaccaatt tgtaaaatcg
      601 ctataaaagc tggcgtgaga atggtaaaat cagggacaat tcagcgtccc gagatgaacc
      661 atgcagtatt acagcgatat tggtttggaa gttcacgtag agttatccac ccgcacaaaa
      721 atgttctgcg gctgcccggt ggttgaccca accgtggcgc tgcccaatac cgccgtatgc
      781 ccggtttgtc tgggcatgcc tggtacgcta ccggtggtca acaagcaagc cgtggcattt
      841 gccatcaaag ccggcttggc gctgcactgt caaattgccg aacgcagcgt tttcgcgcgc
      901 aagaattatt tttatcccga ccttcccaag ggataccaga tctcccagta cgaactgcct
      961 atttcctatg agggatggat cgccatcaat tcctcccagg ggaaaaagac cattcgcatt
     1021 cgccgggcac acctcgaaga agatacggga aagttgaacc actgcaatca agaaagtgaa
     1081 ccccattcac tggtcgacct taatcgcgca ggcattccct tgctggaaat tgtgagcgag
     1141 cccgacatgc actcgatcga agaagtgcgc gcctacagcc aagaactgcg cttgctgctg
     1201 cgctatctgg gagttaccag cggagatatg gaaaaaggcg ccatgcgttt tgaagccaat
     1261 gtatccatgc gcgccaatca gcaggaagcc cttggcacgc gcgtggagat caagaacctc
     1321 aacagcattc acgccatgga aaaagccatc acgtacgagt tggtgcgtca gcagaagatc
     1381 ctggaagaag gggacgtggt tttgcaggaa accttggggt ggagtgagaa ccgttcggaa
     1441 actttcacgc agcgcagcaa ggaagaagcg catgattatc gctattttcc cgagcctgac
     1501 ctgccacctt tgatcgtcga acgagcctgg accgaaagcg ttgctgctga gatcccggag
     1561 tttccagagc agaaacgtgc acgctatcag agtgagttga acctgccgcc tgataagagt
     1621 gaagcattga ttcaagacaa agcggtcagc gcttattttg atgaagcgct caaagctgct
     1681 gaggacatcg ctgccgacac catcgccaat tggatcatag gcgatttgtt cgcctggttg
     1741 aacgaatcag gctgttctat tcaggaaata aaggtctgcc cccatgaatt ggtgaacctg
     1801 atccgtttca accagcaaaa agtgatcaac aagaattccg ccaaagagat tctacaagac
     1861 atgctgcaga gcggaaaaac tgcacaagag gtcattcatg aaaaagggat ggaacagatt
     1921 tcctccaccc tcgagctgga aaaggtggtc acgcaggttt tgcagcgtta tcctgccgaa
     1981 gtcgccagct accacgccgg aaaagaaacg gtgttcaact ggcttttcgg tcaggtaatg
     2041 caagctacgc aaggaaaagc cgatccgcag aaagtgcggg agatcctcaa aaaagctctt
     2101 gcgtaggatc agacgccagt tccccatctg ttatccgcaa gacatcatcc cgattggaga
     2161 gtttcttcat cttgacgcac gcatggttcc ttataaacta agggtataca atcagggcaa
     2221 tcaagatcga accccgtatg aatatcgttc atcttttgtc ttgcttaaaa ccaaagttat
     2281 tggagagaaa gtagcatgaa tgaaacaacc aatgcctttc aaatggctca acagcagttt
     2341 gaccatgtgg cagaacagct aaggttggac ccccaggtac gcgaatattt acgctggcca
     2401 atgcgcgaat accattttcg tattcccgta cgcatggacg atggaacgat aaaatcgttc
     2461 gaaggtttcc gcgtgcagca caatgattct cgcggtcctt gcaaaggcgg gatccgcttc
     2521 cacccttctg aaacaatcga tactgttcgc gcgctggcaa cctggatgac atggaagtgt
     2581 gcggtcgccg atatcccact ggggggcggt aaaggaggcg tgatcgttga tcccgcaacc
     2641 ctcaaggttt cagaaaaaga acgcctgtgc cgcggttata ttcagaagat atggcaaaac
     2701 atcggtccac gccttgatgt acccgctcca gatgttggca ccacccccca aatgatgggc
     2761 tggatgatgg atgaatactc caagctggtc ggcgaataca cccctggcgt gatcaccgga
     2821 aaacccgtcg gtggtggtgg atcgcagggg agaaccgaag ctaccggcta tggcgtcatt
     2881 tacaacctgc gtgaagccat gttgcgcttg aagatggatt ctgccaaatg ttcggtcgcc
     2941 attcagggct ttggaaacgt agcacaatat gcggctatcg gttttaaaga attgctgaaa
     3001 ggcaaggtga tttgcgtttc ttattgggat cgccacgatc gcacctccta tgtggtcagc
     3061 aatgaagatg gcattgatgg gcgcttcttg caatctatca ccgaccagta cggcaccatt
     3121 gacaaggaaa aagccaagac tgccggatac aaaatcgaag acggtagcgt ttggctgcaa
     3181 aaagatgtcg atgtgctcat tcccgccgct attgaagggc agatcaatgc cgaaacagta
     3241 ctgcaggtca gcgacaaagt tcgcatcatc gccgaaggcg ccaacggtcc caccacgccc
     3301 gaagcggatg aagtgttgaa gaaaaacaac gttttcgtca tccccgattt tctgtgcaac
     3361 gcgggtgggg tgaccgtctc atactttgaa agcgtacaga acgacatgaa cttttactgg
     3421 acgcgcaaag aggttttaga gcggctggat gataagatca ccgaagcctt ccagagcgtg
     3481 tatgaaatga gcgaaggcga aaaagtattc atgcgcgatg cagcttatat ggtcgctatc
     3541 aaccgtgttg tcaccgccat gcagttgcgc ggttggatat aagccgacgc ttcttgaacg
     3601 aaaagccaga tgaaactgtc tggctttttt tattctcgtt atttcccaca ggtaagggaa
     3661 tggctaaaac agtggatcat cgaatgcatc cgagtaatac tgcttcgctt tgggaaccgt
     3721 atccaccgtc aagatgtaga ccctgttttt ttgttgccga caaaggtgag caattcatcc
     3781 atctcggcag ctttgacttc cttgggtagc ggcacctctg gcaagctgac ggcactggct
     3841 tgaacccata aggagacggt acgatgatga ataccaaaat ggcgggcaat gcggcgcaaa
     3901 ttcatgccat cgactttata tccaccccca aaaaattttg ttgatctatg ttatcgctta
     3961 ttctttggga tatatctttt tgccacgtaa atctcaaaaa aaggaatggg cttgtataaa
     4021 gttttcagag aggaaacgag aaaatgtttt tccatttcat cctttacatc gtaaacgttt
     4081 ctagtatggt agtgaccact ataatttgct aaccttcttc cgattttgta aaaaatattt
     4141 tctgttggac cggaaacgag aataaagcca tctttcgtca ataactcaga aaaacatagt
     4201 aggatttctg acaaattatt aacatgttca agcacatcaa gcgccagtat gaaatcgatt
     4261 tttccttcga acccattcaa ttgctccagg ctgtcaagat aagcaacatt ttgccatccc
     4321 tctttttccc ctatttcctg caatagtgaa atgtcgagat ctaaaacaaa caaagattga
     4381 actttttgtg ataaatatgg aatcatcgtg ccaagaccac agccaaaatc caggcaggta
     4441 ttgatcttgt taaaactaga tagatatttg attacaaacc ttattctttc ccaaaataaa
     4501 tatcgcatca gagggtttgg atttgtgtat gaaggaagcg cctgttcgtc aaattggttt
     4561 ttgggatatg tatgcaacat ttctttgaaa agggtgattt ggtgcttttt gtactgagaa
     4621 aaatctttgt tagtggcgaa catcactttt tacctttcaa ggattacgat ggaatcgtct
     4681 atagagttct tctttataag aatggaaaat cttagatata ttataggcta atagataatg
     4741 attatgtgaa tgaagaaatt atctgcttga atgagcaggt aacatcaaac gtttcccgag
     4801 cgcatctcac ttcgccagtt atggtggaac agtacctacc attttttcca gcggagggaa
     4861 aacacgccag ggtcgcatgt gcaaacaagc taatcaatac ctgaaatggg cttttgtgga
     4921 agcagccaat gtgatcgtat tgaatcatac acgttccact ccccgggtaa caggcgctgg
     4981 aaattttaag tcgctcgttg tatacttttt acaatccgaa caatccggga gggagtaatc
     5041 gcatgaaatt atctgtctcg caaccacaac ttgctcacgg gcttggcatt gtcacacggg
     5101 ctgtttcccc ccggagcacg ctacctgtct tgaacaatat cttgcttgct accgatgaag
     5161 gtcgtttgcg cttatctgcc accaacctgg aattagggat caccaaatgg atcggtgcgc
     5221 agattcagga agaagggtct atcaccatcc cggcgcgcat tttttctgac cttgtgggag
     5281 cattacccgg aacgaccgta gagctgacat taaacccccg cacacaaacc ctcaacgttc
     5341 attgcgggac ttccaatttt gatatcaaag gcattgacgc gcaggaattt cctcccatgc
     5401 cagtgccgga tctacaaaac agtgtggatt taaacgtttc tgatttcaaa gagatgatcc
     5461 agcaggtcag cttcgctgca tcgaccgatg aggcgcgccc ggtcttgcag ggtgtgatga
     5521 tggagatcgg aaataatgag gtcaccctgg ctgccacaga cggattccgc atttccgtta
     5581 aaaaagctgc cctttccacc ccgcttgctt atcctgtgaa agtgatcatt cccgcccgct
     5641 cgctgagcga attggcgcgc attgccacca acggtgacga atccatcaac atggtcgtgc
     5701 cagacgggcg cggtcaggtg attttccacc tcaaggatgc cgagctggtg tcgcagttga
     5761 tcgatgggaa attccccgat tacaacgcca tcatcccgcg cagtttcaaa accacaacca
     5821 ccctcacaac cgtggaattc cagaaagcct gccgccaggc agagatcatc gctcgcgaaa
     5881 gcaacaatgt catccgcctg cacatccaac cccaggaaca gggacctgga attgtggaat
     5941 tctcctcaca atcagaagaa cgaggttccg gcgaggtggt gctggaagcc aacatcgatg
     6001 gacctgagct gctgatcgct ttcaatgtga gctatctgct gaatgtattg gacgtggtct
     6061 ctacacccaa tacgattctg tcaaccaacg ccgataacac tcccggcttg atacgaccgg
     6121 tcagcgacga ttcattcttg cacgtaatca tgccaatgca cctcggataa tcgggagttt
     6181 tgtaatcgag ccacgctttc gcattttgtt ataatcgctg cacagaatag aagcttcctg
     6241 tttctctatt aggttgtgtt ttgtttattt ctggctgcag cttcttttct attacagaac
     6301 aaggaagtcg aaaatgcagg atttttccaa ttccaccgac cgtttgatga gcatcacgct
     6361 ggcaatgggg caatatccga ttctcagcga tcgtattcac agcaaaatgc gccaggaact
     6421 gttccgccgc aatatcatca cccaggttgc gctggaatcc gaagtgcgtg aaaaagccat
     6481 ccaatcgcag gaacgcgagg gcgtgaataa tccgctgggt gaagaatcag ccgaaatgtg
     6541 ggaaaaacgc accacccgcg tgcgcgacca gctcaccgat ttgctattca gccagcactt
     6601 ttcttttgac ctgtttcagg agatcgtcac ccgtgttctg gtggaaagcg gcatttccac
     6661 cgagcggcac aatatcgact tcaaccccga actggcaccc caagaactgg tctttgaaca
     6721 tgcctggtcc attctgcgcc ttccacccga cgagcaaaaa cagtacaacc accatttgga
     6781 agaatccgta gtggtcttga tccgcaccat gatcagcgac cagcttccct atatcaatat
     6841 agcaaaacgc tggttcacca tccaggatct ctcagagatc agaaagagaa agatcggaac
     6901 cgggcgcatc ggcggcaaag ctgccggtat gctgctagca caccgcatcc tggaaaatga
     6961 attggatgat gatctcaaac cgctgctcgc aaaaccagac tattatttca tcggttcgaa
     7021 cgaacaatat tcattcatca ccatgaaccg cttcgagcgt tggaatgacc aaaaatataa
     7081 aactgaagag caaatgcgcg aggattatcc aaagatcgtc gatgactttc tgaaggggaa
     7141 attcccaaac gatattctgg aaaaactcga agtgctgcta cagcaggtcg gcagatcacc
     7201 acttatcgta cgctcatcca gtttgctgga agactccttc ggcacttcct ttgccgggaa
     7261 gtatgatagt ttcttcttgc ccaaccaggg cacaccagag gagaatttat cagccctcac
     7321 ccaggctatc accaaagttt gggcatccac gttgaacccc aatgcgctgt tataccgccg
     7381 caagaagggg ttgcaggatt acgacgagcg catggctatc ttgatcatga aagtagaagg
     7441 ggaacgtttt ggtaaatatt atttgccaga tgcagccggc gtggcgttca gccgcaacct
     7501 gtaccgctgg acaccccaga tacgcaggga agatgggttc gtgcggctgg tgtgggggtt
     7561 gggcacacga gctgtagacc gggtgggaaa cgactacccg cgcctgatcg ctctcagcca
     7621 cccattacta cgccccatgc ctgacttcga aaccgtcgga cgctgctctc aaaaatttgt
     7681 cgacctgatt gacatggaaa aaaatgcctt ttccaccttg ccaatccacg aagtcatcag
     7741 cagcgattat cttccattga aatatatcgc ccagctcagt gaagaggggt atttttcgcc
     7801 catgcacagc cgattgatgg agaaagaccg caatcgcatc gtgcttacct ttgacgaact
     7861 gctgcgcaag accaattttg cggtaagcat ccgcaagatc ctgcaaaaac tggagaccaa
     7921 ctacaacgca ccggtcgaca tggaattcac cctcaacatt gaagagcagg aagcgggcaa
     7981 tccgaccatc caatacacca ttctccagtg ccgcccacaa agccaccttt cagatagccc
     8041 cgaaggtcag atccctgcaa atttgcagga gaaggatatt cttttttcca cctatttcat
     8101 ggttccgcag ggcatggtta ccaacatcac ccatgccgtg ttcgtgcccc ccgaggagta
     8161 ttacaaactc gatgaatccg gtcgcttcaa gctggcgcgc gctatcgggc aactgaactc
     8221 taaattagca gaaaaacaat atgtgctgat cggtcccggc aggtggggca gcaccaatac
     8281 cgacctggga gtgccggtag cctatagcga tatattcaac accgccgccc tcatcgaact
     8341 atccgggaaa gatattggag cggctcccga accatcgctg ggtacgcatt tctttcaaga
     8401 cctgctggaa gcacagatct acccgcttgc aatcctggtg gatgacccag aaaatgtttt
     8461 tcaaaagtct ttcttctacc atgttcccaa ccggctgggg gattacctgc ctgccgacga
     8521 ctggttgaaa aacatcttac gtctgttgcc catttcgacc taccgcaaag gtaaccatct
     8581 caagatcatc atggacgaag agaagagtta cgcagcgggg tatttggaac cagacaagaa
     8641 agaataagca agctgaacaa aatacgcagg tcgagccagg tattcaacgc tacgtaaata
     8701 gaatatatgt tctatagcta ttctaccgcc gtctcctgca aaagatctga aacgctcacg
     8761 aaagtgtagc ccattgcttc cagcccgttg atcgtatcgt aaaaagcctg ggatggttgc
     8821 cagtgatgga acataaagga tactacccca tctctgacca ccgaatacat atcggcaagg
     8881 gctagttttc cttctggcgt aggcgggact ccgaattcgg catttccccc ttcttccaga
     8941 taaccaaggt tttctggcaa aatgcgcact ccatagatcg aatcaaagat ttgatatggg
     9001 aaagcttggg tgatataggg catcttggag taatcgttgt tagaatctgc tgttgcttca
     9061 ttcagatcgt tgaaaacttg tacgcgctca tagatgacat cgaaggaatt cttgatcgca
     9121 aaataagtat tgggagaagc cgaataatga ggggtttccc agataacggg ggcgtatccc
     9181 atggcttttt caaactgttc cacaccagct ttgatccgtt catctgccca ggaataacca
     9241 tcttccggaa aaggagcgtc attctcgcca tcccaaaatt catagtcgac tccgctgacg
     9301 gaatcatgct ggtgagtgta tccgtgcagt acaggggtgc cgccacgttc aaccatataa
     9361 tcgatcagct cttgcagcat gaaatcttca tatagataaa ctgtcttgcc gggctctccc
     9421 catatccctt cggggtcggc attgaccgga ataacgccaa aggcaaaagg gatgcctttt
     9481 tcatagagaa agtccacctg ttctttcacc cgctcataat tggtattccc tggcatcagg
     9541 tcctcaaagc gcaccagcgc ccggtgattt tcttgcacag tcgtttctag cagatcgtgc
     9601 agcaaatcgg cgaacaccag gtagctggca tagaaatttg ccatagggtt atgggtgata
     9661 taaaagaaat ttccacatcg gatagcgtag ggttggtcga ccgcgccttt tatctgcaga
     9721 cttgccagtt gctcgcattg agatccggca tcgatattga ccaggttgaa gtatacatcg
     9781 gtctcaaggc gcggcagttc ttttcctttg tagataattt tgatatcagg attgacgatc
     9841 tccgcttcca catactcgaa cccaaacttg ctgctcgcgc cccactcttc attcctgaac
     9901 agttgccata tattggaatt catccacagg aatggagtgt ctgtgctgat gacatcctgc
     9961 aaaaaatcat cgctcagggg ataatcataa atattaccga tataaattcc tgctgagaaa
    10021 tttgccagcg ccccagcccg gtaatcagcg atatcgagaa tggtcacttc gcgctcgaaa
    10081 tgaccgagaa gattgcgcac catagccgca tagatcttgc caaagttcca tgggtctgcc
    10141 gggtcgtcgt catacaagac aaccaccttt tttctcgtgc tcagatcgcc gctctcagca
    10201 atcgtgatct ggttgaccgc aggtatcttc gctctgctac aactcgccac aaggatcagg
    10261 caaaagataa ccagcaacag ggaactcttt ttttcaacct ttcgcttatc ttttttcatt
    10321 gcatccctct ttgaacacta atcggtcaat aatttttgcc ggttaaaaga tttgacccct
    10381 acccgcccaa tgatcgcagc aataccccaa aatacaaagc ccatgatcag agccaccagc
    10441 ggactaagga acagcacacc ggtcatttga ccaatgaaaa acacaaccac aatcaataca
    10501 atcgaggaac tggtctgata agcgccctgg aatgtttgag ctttggtcga aatgagcaca
    10561 gtgagcgata tgctcatggc gatcaatgcc ggtgataccc aaaagatcaa aagccaccaa
    10621 accggcagcg ggaaccacaa ccttccaaaa ctggcatagg gaaccccatt cacaataatc
    10681 gtataaagga aaaaacagac ccaggtgatc agaacggctg gtatgcctgc cgccatgacc
    10741 tttcccagga ataattccag atcgctgatg ggcagataca gcaatgcttc cattgtcttt
    10801 ctttcttttt cgcccgcaaa tgactcagcg gcaatggtgc tcgaaaacat taaaggaacc
    10861 atcaggaaca tgggagcgaa aatatatccc agcacagtta cgatcacaat ttgggcagca
    10921 tccaaaccgt ccaatactct tttcatttct gcgggcatgc ttgcaaaaaa gacttgcata
    10981 tctggatcgg tggcaaaacc ttccaccgcg cccgccacat tgggcaagat cagggtgatc
    11041 aaaagcggga atatcacaat gaagatcaag gggacaatca ggattggaat aaaaaccgac
    11101 ttattttgtc ttacttctcg ccaatctttt tgtgcaatgg taaagctatt tcctatgttc
    11161 acgattcacc tcaccagccc ttgcagcttg aaatagacct cttccagaga tggcacctgg
    11221 gggatgaccg aaagcacagc cagacctttc ccgatcatcc aggtcagtat ttcgggcacg
    11281 cgctcatcat ctgaaacgtc cagcaacaac tctttttctt tgacttccgc atttttgacc
    11341 gcttccaagc gggataattc cccacctatc cccgcaggca agggtttttg aaaagaaaaa
    11401 tgcacccgct tcgccaaacc cgcggcttcg cgcaacgctt caagcgtccc ttctgccaac
    11461 atcttcccct catgcatgat agcagcatga tcgcagacct gctctgcttc atataagcgg
    11521 tgagtgcaga tcaccacgct acagccttct tcccgtttaa ctttcaaaat caaatcctgc
    11581 acctggcggg ctatctcagg gtctaagttg gaggttggtt catccagaaa caacaattca
    11641 gggtgatgca gcaaggcccg ggcaattgcc aggcgctggc gcataccgcg actgtaggtg
    11701 ctgacccgct ggttagcgtg cgcggaaagg tcaaaataat ccaacaattc ctgaatacgg
    11761 gtgttcaaat ccgttttgct catgccattc aattttccga agaaggtaag gttctccagc
    11821 gcggtcagtc gttcatataa tgccggcgtt tctgtgagca cgcccgttct ccggcgcacc
    11881 gtgacgccat ctttataagg atcgcacccc agaaccgata tgctgccaga ggaagcatgc
    11941 aataacccgt tcacaaggcg aatagacgtt gtttttccgg ctccattggg acctaataaa
    12001 gcaaatatct cccccttttt catgcgaaaa gttacattgc gtagcgcaaa accttttcca
    12061 tattgaaaag aaacttgatt caaattgata acgtccatag ctcgctctct tctcaggttt
    12121 ccaattataa atcgaaaaga tcgcctttgt ttcacgtgaa acaggatgat tggcttttca
    12181 gggaaatgtt tcacgtgaaa cataacgcca gccttgactt tcttcacttt tcccctttca
    12241 aatcaagctt gcgtggtaga atgtggttgc agtatttcag gtattggagg tatgtacaat
    12301 gagcgcagaa gaaaaaggca ccttcaaatt aaaagcgggg ttggcgcaaa tgctcaaagg
    12361 gggcgtcatt atggacgttg tcacccccga acatgccaag atcgctgaag acgccggggc
    12421 agtagctgtg atggcgttgg aacgggtgcc tgccgatatc cgcatgcagg gtggcgtagc
    12481 tcgcatgagc gatcctgagc tgatcctcaa gatcatggat acagtttcga tcccggtcat
    12541 ggcaaaagcc cgcatcgggc actttgttga agcgcagata ctggaagcgc tggggattga
    12601 ttacattgat gaatcggaag tattaactcc agccgatgaa gaacaccaca ttaacaaaca
    12661 cgctttcaaa gtgcctttcg tgtgcggctg ccggaatctc ggagaagctt tgcgccgggt
    12721 cggcgaaggg gcagccatga tccgcactaa aggcgaggct ggcaccggcg atgtggtcga
    12781 agctgtgcgg cacgctcgcc gtgtgctggg tgaaattcgc acattgcaga acttgcctga
    12841 tgaagaagtg atggcttacg caaaggaatt gggcgctccc tatgaattaa tgatggagac
    12901 ccgtgccttg gggcacctac cggtggttaa ctttgcggct ggcggtatcg ccacacccgc
    12961 agatgctgcg ttgatgatgc aattgggcgt ggatggcgtt tttgtgggct ccggcatttt
    13021 caagtccggt gatccagcca aacgtgcggc ggcaatcgtc aaagccacca cccactacaa
    13081 tgatgcaaag atcctggctg aggtcagccg aaatttggga gaacccatgg ttggcaggca
    13141 ggtttcctcg attcctgagc aagaattgat cgccgatcga ggctggtagc agaagcgcat
    13201 gaagatcggc gtgttagctc tgcagggttc gttttatgaa catcagcaga tgctgcagaa
    13261 tttgggcgtt caggttgtgc aggttcgcct gccgcacgag ctggaagggt tggatgggct
    13321 tatcatcccg ggtggagaat ccaccaccat tggtaagctg gctgtcgatt ttggcttgct
    13381 tgaaccgttg cagcgctttg gcaaggagca cgccatttgg gggacttgcg cgggcagtat
    13441 ctttttatca aaagacgccc accgccagca gccattgctg gggctgatgg atattcaggt
    13501 ggaacgcaac gcttttggtc gccaggtgga tagctttgag attgacctgg aaattcctgt
    13561 attgcgctcc gtcaatcatg caaaacccat ttttcacggc atcttcattc gcgctccttt
    13621 gattgaaaaa gcggcggaag gtgttgaaat tttggctcgc ctgccgaatg acaagatcgt
    13681 cgcagccagg caggggcatc ttttggcaac ctcatttcat cccgaactta ccgacgacac
    13741 acgctttcac cagtattttt tggaggtggt tccatagata tcaggtcgtt cagcgctgtg
    13801 gaccttcccg ctatgttgca gaacacccac aacgttctat gtctcgacat ggaacgtatt
    13861 tttttaaggg gcattcaaga tccagcgcgt gattttttga agaactgctt tctttcaaaa
    13921 gaaagcatct tttcttacag agcgcaagaa gatggcgctc cggcatttgc gcagttgcag
    13981 tttggcgaaa ggtctgcggt attcctatac tatatttcac tgcccggtac atcacccgca
    14041 aattcaatag aaagtctttg ccggattgcc ggtgaagccg gaaaagccca cctgcttgct
    14101 aacgcaccca tgcatagcga gcagtggcag ttcctgcatg ccatgggatt ccacacatac
    14161 tcggtacaaa atatttggca ggtaaatgat cttcctgccg ccagagcatt tagcggacag
    14221 tggtgtattg aaaaagatca ggaccgcagt gccatcactt cttattattc gcactttctt
    14281 tcaccacttg aactggcatt tcaaacctgg aattttccgg atatctttca cctcatactg
    14341 cacgatcaca gcgcatgtgt gcgaggtgtt gcgcgcatcc gtttcttcgt ggatcgggca
    14401 gtaatattgc cgatgttgga tttgagagaa aacgacgaag gtgaagatga ttgcatgatc
    14461 gccctgctga aagaatgttc gcggtacttc tccaaattat ttatcagaga atccaccagg
    14521 caccccatca acacaaacat ttttgccaaa agagaagaaa cctgcttaac ggaagatcac
    14581 tttatggtga gaaatctggt tgccatgaac gcaatcagaa attaccagcc gttggaattg
    14641 cttgacgaaa agggaatcgc caaaccgacc actccttttt cccattcctg aagataccaa
    14701 tatcttcgcc ttatcgattc gattcatgtg ataatatgaa agaataatta tgaagcaaaa
    14761 ccatatcacg gatgatctat ccgccttatt atccgttctg ccttctgagt tcaaccgggc
    14821 actggaacgt gagaaccaga acgacgatct gttggaaatt atcatggatc tgggcagggt
    14881 gccagttgcc agatacatcg atcatgagct cactttaagc gaaaaagaga tcacccatga
    14941 tatgatccag gacacggtct cgcgcattgg ggaattcgac ggtgacaatc gggcaggtat
    15001 cgaacgcacc ctgcaccgta tttcagctat acgcaataaa aaaggcgata taatcgggct
    15061 taccctgcgg gtcggtaggg cagtttacgg gaccattgat atcatcgaag atctggtgaa
    15121 atcagaaaag agcatcctgc tgcttggaaa accgggcatc gggaaaacca ccatgttgcg
    15181 cgaggcagcc cgcatcagag ctgagaattc gcgcgtcgtt atcgtggaca cttccaatga
    15241 gatcggcggc gacggcgatg tcccacaccc cgctgttggg cgagcacgcc gcatgcaggt
    15301 atccaatcca ttgctccagc atgaagtgat gatcgaagcg gtcgaaaatc ataatcccga
    15361 agtgatcgtg atcgatgaaa tcgggcgcga aatggaagcg ctcgctgccc gcaccatcgc
    15421 cgaacgcggt gtgcagttga tagcaaccgc ccacggacgc acactggaaa acctgctcct
    15481 gaaccccacg ctttcagatt tgatcggtgg cattgaatcg gtaacgcttt ctgacgaaga
    15541 agcgcgcagg cgtggaacgc agaagaccgt gctggagcgc cgtgcacccc ccacctttga
    15601 cgtattgata gagattcaag atcgcgacca tcttgccatt caccaggacg tagccgcttc
    15661 ggtggatgcg ctggtgcgcg gttatccgtt ggaaccgcag atccgtatgc gcgacgagag
    15721 cggcaaaatt aacacgcagg agccgcaaca gcgtgttgaa accgccaatg cccaggcgca
    15781 gggactgcgc cgccaaaaca ataatggaag ctatggcaac gaagaaaagc agctccgctc
    15841 ctccaaccgc cgttttgaaa gcgctgccaa agaacagcag gcgttattac cccctataaa
    15901 cgttttcccc ttcggcgtag ccaggaacag actactggaa gcaattcgcc gcctggggtt
    15961 acccgcaaaa gtcgtacacg accttcagga tgcagacgtt ttcatcacac tgaaatccta
    16021 ttatcgcagc aggcaaagac ccatcttgca ggcagaagaa aggggtattc ccatctacgt
    16081 tctgcgggca aattcggaga accagatgga acaggcactg gcagagattt tcaacctggg
    16141 cggaccgagc ggcagacctt tgaattacga ccgcatcagc caggaagtgc acaacgccgt
    16201 cgatctggtg ctggctggca agcattgggt tgaccttccg ccagcctcag ccaaaatacg
    16261 ccgcattcaa cacgaacttg ttcgcgaagc tgaattgacc tcccattctt atggaaaaga
    16321 acccaaccgc tatgttcggg tttttcgtga gtgatgccca tgttcattac gcttgaaggt
    16381 cccgacggca gcggaaaaac tcagcaaatt cagttgctgt cggcattttt ggaatccagg
    16441 ggacagaagg ttttcttctc ccgtgaacca ggggggacgg agatagggga taagatccgt
    16501 agcatcttga tggacatgca taataccagc atgcatcccc gaaccgagac actgctcttt
    16561 tgtgcttcgc gcgcgcagat cgtagaagaa gtgatcaaac ccaaactgca agcaggatat
    16621 atcgtgatcc tcgatcgcta tgccgattcg accattgcct accagggata tgggcacggg
    16681 cacgatctga ccatgatccg gcaattgttg gcttttgcaa ccggcgggtt gaaacccaac
    16741 ctcaccatcc tgctcgacct tgatccgcag gtcggactgg aacgtagaaa acaaggcggg
    16801 ggagaatgga accgcatgga cgcataccaa cttgcgcttc ataagcgcgt gagagcgggc
    16861 tacctggaaa tggctgcaca agaacccaac cgttggcaca tcgtctctgc tgaccagcca
    16921 gttgaagtgg tacaatctag tattcaaaga attatattag attatttaga gtaaaattgt
    16981 ccgcatatgt tcagaaaggg aattccaatg cgtaaatata tggttgtttt tcttttgctt
    17041 gcgatcctgt tcactgctgc ctgttcaaaa actggggtgg cagacgcaac tcaggttgcc
    17101 agcgagactg caggtgattc cgaagccgcc gaggcaacag tggcaccttt tacatctgcc
    17161 gatgaaagct gtaaaccgta taatctgatc gatgaccttc tgcttcaacc ggttgatgca
    17221 aatgttccag ccgtgacaga tgccgatttt atcgagggtc cggcagacgc aaaatacacc
    17281 atcaccgttt atagcaactt tacctgctct cactgcgcaa acttggaacc gatactggct
    17341 cagctccaga cactgttttc aaataccgta cggatcgtct tccgttatgt aactaccggc
    17401 ggcaattcga atgtcgcggc gcaggctgtt gaagccgcca acatccaggg caaattccag
    17461 gagatgaaaa atgtcctctt tgaaaatcaa gctacctggt attcttacag cgaagacgac
    17521 ttccgcgact ggttgggtga gcaagccgct tcctttggca tggatgtcga tcaattcgac
    17581 accgatatag attccgagac cacgctcaac aaggtcacag aaaatcgtac cggggtggat
    17641 gaattgggaa tttatggaac gccaaccatc tatgtcaata accgccaata cacccagacc
    17701 cgcagcgttg ccactttcgc gatcatgctg gatgtaatgg atatttctga tcaactgctg
    17761 ggcgattgcc caattatcag caccgatttt accaaagacc tgcaggcagt gatctccacc
    17821 agcaagggcg atatcgttgt ggacctgtac gaagatgaag ctcccaatac ggttgcgtat
    17881 ttccagtatc tggcagaaaa aggctggtac aacaataaca acatcatcat ttcaaccgcc
    17941 gagtattcca tcagcggaga tccttccaac accctgtacg gtgggccggg attcgcatac
    18001 ttcaacgaat tagccactga tcaaacgctg aatgaagaag ggttgctggt ttctttcaat
    18061 cagttaggta cgggatacaa caccggcata ttcatgctga ccaaatcagc cgtcaccgat
    18121 ttcaccggtg aaatttcgat ctttggaaag gtcatcgaag gcatggatgt gttgaacgct
    18181 tttaccgacc gcccttactc actggatcct gctgaacctt tctacgatag catcaccagc
    18241 attaccattt ttgaaaaatg atagcggatg agcgaagaaa tcgcgattgg ttgaagatca
    18301 gcctgttcat tttcagcctg accgcatggt tatttacaac agttagtgcc gccagttgtt
    18361 ttgctggcgg cattgttttt cgcgcaacac ttggtctttc atctgaccaa acagccatgg
    18421 ttctttcgct tggttctctg agtgttttgc tgtcagtgat tcatttttca gccctgcgtc
    18481 atgccttcaa gaattccccg ccagctccaa tgcccacgcg ctcagacaaa gcattgtcca
    18541 tttcctcagc ggcgatgttg tgttggatat tggttatggg tataagctat ttcaacggta
    18601 acgacaaact ttttactgcg atacagcctt accttacccc gctggcaatc tggattccga
    18661 tttggtggtt catcgagttt ggaaaaagga aactgccatc catcacggca cgcaaacgaa
    18721 acgccgccct gagcattggc tccagttacg tgattgtttt catcatggca ttggaattga
    18781 ttgtctttgc gctgttaata gccgggctgc taatctattt gaacacccaa ccatccatcc
    18841 aacagtggat caggacactt tccattcctg aagacctatc ccaaatagac acgcggttta
    18901 tcgagcaata cattcacagg attcttcaaa acccctggtt cctggcgata gtttttatgg
    18961 ttatcggggt catcgccccc ttcattgaag agagtttaaa gcctgccgcg ctgtgggtgc
    19021 tgcaaaagcg ttctctcaca ccaagtcagg gtttcatcct gggtctgtat tttggaggcg
    19081 cttttgcgtt gattgaaaat gccggtatgg tgatccaact cggttcgcaa agttggtctg
    19141 aaagcgtcct actgcgcgca gctaccgccc tcctgcatat aacctgtagt gggctggttg
    19201 gatgtggtta cgcgcgttct atgcagccgg gcaaaaccag tgcatttctc aaaccgttct
    19261 tattggcgct cctacttcat ggcgcatgga actcaatggc agttctttcc ggttacgctt
    19321 tcttttcttc tgaaacaggt attgacgcac tatcctccac ccccgcactg aagttgatct
    19381 ctataatcgc tttggtcctg atatggctga tcattgtata cttactctgg aaaaagaatc
    19441 aggatctcag aaaggaaaca tcatttgagg cgcacacaaa tagtgcagat ggggtttcaa
    19501 tgaataaagt agaataaatc aggagaagtg tcagtgaaga gatcaaccct gcagcatatc
    19561 gtccgctttc ttatgcgcgt gctaacgagc accgaattct tagggttgga aaatatcccg
    19621 actgagggag gggcgatcat tgctatcaat cacttatcct acattgatat ccccttgctt
    19681 tttgtcaatc cgcgccgacc cgatatcaca gcactgatta cgaccaagta caaaagccat
    19741 ccattcattc gttggttttc tgaaaccgct gaaggaatct ggatcgaccg tgacatagcc
    19801 gatttcacgg ctatccgtga ggcttcgctg gctctcgcac agggaaaggc tctgggaatt
    19861 tctcctgaag gtactcgcag cctgagtcat acgctggcag aagggaagca gggaacagca
    19921 atgctggctt tgaaaagcgg ggttccgatc gttccaaccg gtattcaagg aacagaagac
    19981 gccatagcga aattgaaaca tctgagaaaa cctcacatgg tcgctcgctt tggaaaacct
    20041 ttcacgcttc cacccatcac tgagttcgat aatcgcgcag agatattaca gcgctatacc
    20101 gaagaaatca tgtgtcgcat cgcggttctt ttgccggaaa aatacagggg ggcatataag
    20161 gatcaccccc ggctcaaaga attcgcgatc taatatgccg aagttggtgc tcctctggat
    20221 ttcagcctct ttcttagttg ggatcatcac ggctggagtg gcaccacttt cattcaactg
    20281 gtcacttggt tttcttttac cccttgtttt ctttattttc tacggcttgg gtaaaaaaag
    20341 agagaattta aagcgctggt tgtttttcgc ccttcttctt ttttcgtttt tcggaggggc
    20401 gctccgctac caacgctttt ccgaatatcc aacattgccc ggagatgcca gcacaaccgc
    20461 tgaggcggtc gtcttgcggg gatccgttgt agagccgagc gaggcgtcta tccaatacag
    20521 cgaggtggtc cttgaaatcc agcaggttgt agacccctct gaaggatggc aacctgcgga
    20581 aggaaaagtg cttatccggt tgcccgaaga attctttttt ttatatcgca cagcgcttac
    20641 cctccaaggc actttgatcc ccgcggtagc tccgggagaa aaagcgcata cctcctggct
    20701 tgagcagaat ggtattcact atcaaatgtt ctacccaacc gttctagaag agacaccctt
    20761 tgaaggtttt tctttaatgg gggtactgca cgcctttcat ggcaaagcac atcgattgtt
    20821 gcaagcgatt atgccctatc cagagagcga gctgttggca ggcattctat tggggattga
    20881 atcaaggatt ccggagcacc tgcgcgaggc ataccggcta accggcactg ctcatatcct
    20941 tgccatctca gggttcaata ttgtcctgat agcgggcatg gtcagcaggt ttcttaatcg
    21001 tcttcttccc taccgtattg ccgccatgtt ttccatcgtc gtgatcgccc tttatgctat
    21061 tttcgtcggt gcgcagccag ccgtcctgcg tgccgccttg atgggcatca tctccatacc
    21121 cgcttatctc atcggtcgca gggtcatcgg cattcactcg ctctccatta ccgcagcgct
    21181 gatggctcta ttcaacccct accttttgtg gaatgtcagt tttcagttct ctgtttgcgc
    21241 taccctcggt atcatgatgt atgcagattt cttcacccgc aaagcagatg gatggcttca
    21301 aaaaagctcc gtcccgtcga aggaattcgt tctgggtttt attaaagact ccgttatcat
    21361 cacattttca gcgcaggtgg caactttgcc ggtgctggca agccagtttg aagaagtgtc
    21421 tttgatctca cccctggtca atctgctcat cctgcccttg cagccgtcag tggcgatact
    21481 gggagggttg gcacttgttg gcgggtgggt cttttaccct atgggaaggc ttatcggcat
    21541 ggctgcctgg ctcctttcag cgtttaccga tcaaataata ctcttctttt ctcagattcc
    21601 attaacagta tccttaaacc cggtctgggg cggctggctc agcgtgggac tgaatggatt
    21661 gttgattttt actgtcctga gggacagaaa aagagcgacc ctccaacctg ctaacattca
    21721 gggctagctc gttttttctt tgccatcgcg tttccaggcg atgatggtga ggtcatcgtg
    21781 gtaggaaggg tcagaattca ccgcgtataa cttctctttt aacatgttca cgatcatttg
    21841 cgggtcagca tttcgatgcc gtcggatgaa agcgtgcagc tctttttcgt caaagacctt
    21901 gtttcgcacc acagcatcca aagcgccatc ggtataaaga aaaagaaaat ccccttcacg
    21961 gagcgtagcc tttttttgtt catatttggc ttgtgcatct acccccattg ccatacccgt
    22021 cggttttaat ttcatcaacg tatcatgttc ccggtcatat aaaaaaggag ggttatgccc
    22081 ggcattcaca tagaacagct ctccgttttc caggttcaac aatccgaaaa acaaggtaac
    22141 gaacatgttt tcatgggcgt cgtgatagat caggtgattg gcttgtgtga tcatctcggc
    22201 tacattttta cggttcatgg caaccgctcg catggtcgtg cgcgtcagtg ccataaaaag
    22261 cgcagccggc atccccttat cagacacatc tgcaattacc aaacccacca agtgttcact
    22321 gatcggaata aaatcataaa aatcaccacc cacttcaaaa gcaggttgcc agtagatcgc
    22381 cgttttccat ccctgatagt gtggggcttc tttcatgatc agaccgtttt gaacatcgcg
    22441 agccatctgc aattctcgct gcatgcgccc tttttcaagg gcatcttttt gcagattcat
    22501 attttcgagc agcaatccaa taacaggcat gactactttt atcgactcca gatgatctcg
    22561 aaggaaaggt tctttcgtct ctgtttgctg taaatagagc aatccttctt ttttatcatt
    22621 cacaaagatc ggaatacaaa gaaacgagtg caagtgaaaa gccgatacgc tcagtaatgt
    22681 tttaaaacgc tcatcggttt gagcatcctg cacgaaaaat atctggttag cttggaaaga
    22741 ttggatcagg atcatggttg gaagcgtgac tttcttctgc tcgacgtccg tgccgttgcg
    22801 ggtgcgtttg atgaaaggga tgatctcttc attttcaacc atgaatagat atccctgatc
    22861 agcagcagtg aatcgtacga tttcatcgat gattttgctc aatttcgctt ctatattttg
    22921 ttcgttttgt gcatgggcgc tcgttcgaag gaaaaaatct accttctcct gaggggtgat
    22981 attcaagtga tcgctcgctt tctccatacc atggaacttg tatccagcgt caatataata
    23041 atatagtaga cctatgttgt cctattaatg ctctattaac aaaaaatcag ctacaattgg
    23101 gcattacttg agaaaggaag atatggattg gcttaaaggt gctcttcgtt ttgagacaag
    23161 gggaaaaggg ctttacgaga tcaccgatca ggttcgatcg gcgatccacg aatggaatgt
    23221 tcaggagggt atctgttttc tatttctcca gcacaccagc gcctcactga caacctgcga
    23281 agcctatgat cccagcgctc gtcgagatgt agaagagttc tacgagcggc tcgcgcctga
    23341 acggcagaac tggtatcagc atacccttga gggttccgac gattctccat cgcacataag
    23401 ggcaacgctt acccaaagca gtttgagcat tcccatcgac gatagccaat tgagtttagg
    23461 tacctggcag ggtatctttc tcttcgaaca tcgttcccat ccccagcatc gccaattgct
    23521 gatccgttgc ttaaaaacat cgtagcatgt ccaacaaaga tccgctcttc attacgaaaa
    23581 cgaaagtcat tttcgccgtc atcctgtggg gaggatcgtt catagccacg aagatgacag
    23641 tcatggaagc taccccggtg gctgtggtgt ggttacgctt tttgatcggg ctgttgtgtt
    23701 tattccccat catgctgaaa aaaggcatgt tgaaaatttc aagctggaaa gaagttggcg
    23761 aatacatcgt tttgggattt gtgggtatca gtttgcatca gtggctgcaa tctaatgggt
    23821 tggtaacctc ccaggcttct accacagcct ggatcgtcgc ctccacgcca ctgttcatgg
    23881 tccttttcgg atggctgttt ctgaaagaaa aattaactcc attgagcggt ctgggggtcg
    23941 ttcttgccac attgggtgtt ctaacggtcg ttagcggggg cgatattgca ggattgtttc
    24001 aaaatggctt cggggcgcca ggtgatctgc tcatcatgct gagttcgccc aattgggcta
    24061 tctattccat tctgctacga aagaccttgc aacgcgagcc tgcagtgaaa accacttttt
    24121 tctccatctt gtttggttgg ctgcttacga gcgttcaatt tttggtcggc gcagaatggc
    24181 agtcttttgc aaccttaagc ctttcaggtt gggcaggcat tttctacctt ggcatctttt
    24241 gcacatttct ggcttacatt ttttactacg atgccctgca aaaattgccc tctgccagtg
    24301 tgggggcata cctttacctt gaaccgattg ctaccacgct catggcgtca gcggttttcc
    24361 atgaacccat aacgctggtt tctcttttgg gtggcgcctt gatcctgttc ggcatcagtt
    24421 tggtaaacaa gatcacgccc agagagatat catccatcga acaggtttag tacgattgta
    24481 tattggtttc cattcattcc tttgttacaa tcaacatacc atccaaaaaa gcaggtgaaa
    24541 cccatgagta taaaagagaa gatcataagg atcatcgagg tagtctcaga tgatcagcaa
    24601 atattgttgg ataatctgac agaaaaagag cggagtgaaa ccggatctgt taagcgctgg
    24661 acgttcaaag atgtgatcgt ccacagcact ttctggtgga acgtgtttct ggaccggttg
    24721 caagccgctg cgcaaggcaa gcaagccaaa gagatgccgg aagatatcaa ccccattaac
    24781 gatgcggtgc ttgaagaaca caagcatgat tcctgggaag tcgttctaaa ggaaaacaca
    24841 cgcgtcaggc aagaggtgtt gaaatggctg gaaaaactca gcgaagaaga cctgaccgac
    24901 acaggcaagt atgaatggat gcgcgggcgc gcactgtatc accaattctt aggggattgc
    24961 tggcacgatg aatggcattt tgcgcgctat ctggctgaac acgatcggct ggaagaaggg
    25021 gttgccatac aggaaaactt tgtggctcaa ttaaagattt tacctgaatg ggaatatgtg
    25081 gctatttata atctggcttg cttttattcc gtctccggca tgaaggacaa ggctatcgct
    25141 actttgaaaa aagccttgaa gatgaatcca gagatgaaaa aatggtccaa agaggacccc
    25201 gatttcgaaa atatccgcaa tgatccggag tacaaaaaga tctacgaaga ataaaatctt
    25261 ctactcaata aagttctggt tgattattgt agctgtaata ttgtattgat ctggggcaaa
    25321 atttgattca caaatgaatc cactttcact ttttgcttat cttgattcca ctgcaaagcc
    25381 aatccgtaaa ttgcccagct tgcagcggta atcgaagtgg caggatccgt cttggatggg
    25441 actcttacaa gccatagctc cagcaatttt tttatttcat ttctaacctg tttttcaacc
    25501 aaaacttcaa actgggtatc ggaggcgatg cggtgggggt tcatctgagc aataaattcg
    25561 cagactgtta tgatcaaagc gcgcagattc tcccggctat aatgacaagc gttcaatgtc
    25621 ttcgtttcaa gttcctgccg aaaggtttgg gcgatgcagg tttcaagcaa agcatgctta
    25681 tcgacataat gcgcataaaa agtggagcga ttgatttccg ctcgctcggt gatatcctgc
    25741 acactgatgg cttgaaattc tttttcagaa agcagttcca tgaatgcact cgttattaaa
    25801 gcgcgggtac gtttcacccg cggatcaacg cgttcgaaag tgggctcttt ttccaacatt
    25861 tttattcttt cgttgcttat ccaacattgc tcggcaattg atggttgacc aacagccgtc
    25921 atttgctata ttatacaaca tatgtcgatt atacaacaag tgtatgtttt tgcaagtgag
    25981 aaaggataaa ccaatggtca aatcaaatct tgttccaaac atgcaatatg cccgactagc
    26041 cagccgtgaa caaatagagc gcactgccaa agctctcgaa aagaatggca tccatactct
    26101 gatagccgaa aatggggagg aagcaaaaaa caaacttttt gaaagtatcc cgactggtgc
    26161 ggaagttttc actgccactt cccgaacgct cgatacgctg gacattcctg aaggggtaaa
    26221 ccagcgctat aactcggtgc ggtcaaagct ggctagcatg aacaatcaaa cccaagggcg
    26281 tgaaatggta aagctgggag ccacacctga atataccgtt ggtagcgtcc atgcggtgac
    26341 agaagatggg acggtagtga tcgcttcaaa cacaggaagt caactggcgg gatatgctgc
    26401 cagcgcggct cacgtggttt gggtggtcgg ggctcagaaa attgtcccca atctagagga
    26461 agcgatcaaa cgcatttacg aatacacttt gccgctggaa gacgaacgtg ccttacaaac
    26521 atacggaatg aacagcaatg tcagcaaatt gttgatcgtt cacaaagaag ttatgcctgg
    26581 gcgaaccacg ttgattctgg taaacgagaa ccttggattc taaaagcaga gggggatgaa
    26641 aaggcggatt ttatcaattt ccactgaggt tgaatcatcc accctattct ttgttgccca
    26701 tgtaccaccg ccgttcgcgc tttttcagcg cctgctggcg tttatggtgt aacctgcgtt
    26761 ccaccgacgc gcggctgggg gtggtagcac gccgggttcg aggcggctga gcagcctgtc
    26821 gcaggatcac cagcagtttg tccaatgcat cctctttgtt cgcttcctgc gtacgatggc
    26881 ggctggaggt gatgatcagt tctccctctt tggtcatgcg cttgccggct aatttcatca
    26941 atcgctcacg caccgcctct ggcagagagg ggctgcggac agcgtcgaag cgtaactgca
    27001 cagccgtggc tactttgttg acattctgcc cacccggtcc ggaagaatgg taaaaaacat
    27061 agcggatttc actctcatca aaatagaacg agtgtgtgat ttcaatcata atttcctgct
    27121 tcggaattga cacgacatct cacgactttc ccggcagaaa aaatgataaa aatgggatca
    27181 cgatacttct tataaacaaa tctcacaata caatcatacc gtctttaata ttaaaaattt
    27241 catccgcatg agaggcaaca tttgcatcat gggtcaccag cagaatagtt acgccctgat
    27301 tttttttcat gcgcttaagt acattcatta cttcctgccc cgtcatcgta tccaaatttc
    27361 cagtcggttc atcagctaag attaatttcg gataattgat catggcacgt gcaatagcaa
    27421 cccgttgttg ttccccaccc gagagttctc cgggaagatg atcaaaacgt tcccccagac
    27481 cgatcatctc gaccaattct cttgccctat cttctaaatc aaaggagagt gctttgcgaa
    27541 acggtaaaag cgggatcatc acattttcca ttacattcag cgacgggatc aggttgaaca
    27601 actggaagac aaacccaatg ttttctctac gaaaatcagc cagttggtca tcgttgagtg
    27661 agtcaacagc cactccttcg aaaaacaatt tcccacgggt gggcacatct aatgtaccga
    27721 tcaaattcaa cagggttgtt tttccacttc ctgaaggacc cattatggca gtgaaagatc
    27781 cctttttcaa ttgcaatgag acatccttca ctgcacgcac cgtacgcatg ctatcgccgt
    27841 aatctttgcc caattgccaa cattccacca gaatatcgct catttcgtta ctcccatgac
    27901 attctgtaca ctctgataaa tcaaagcggt cgccctgacc agatcttttt cctggatggt
    27961 agacaggtca tcgtattcgc ttcctagcat atcattgccc gcaccgtaaa tgccaatggt
    28021 tggtagatca tcggataaaa gaatcgagag cgataggggg aaatatcctt cgtccagcga
    28081 gcgcgtaaga ggaatatcca gcatttcgcc agcctgttcc acggcggatt gcaaggctgc
    28141 cgggctttcg ctcagcccca tacgccgggt cacctgttgc gtgaagttct tcagcatatc
    28201 gtcatcatcc agatcaacgg acttcgccca atacgtttgc acaaaatact gcccctcgtc
    28261 ctccaagcga gtagcgtcaa cataagttcc catttcatcc agatattcac tgaccaatat
    28321 tttctcccaa tttcccgtcc aggcaatatc gaaggcagcc ataacatggg aggcttccga
    28381 ggcgtggagc tggagataag cgatcaagcc gctgtaaata cccccttgtg ctggcaagaa
    28441 aatgaagcgc acgctgtatg ccggggggtc atttttaaac atacgggcta gttcaagcat
    28501 agtcgcaata ccgcttgcgc tttgatccgc gcccaggctg tttgatgaaa aaccgtaatg
    28561 ggtaatgatc caaatttcct tttctgggtg ctcctttcct tgaatgactc cgctaatcgc
    28621 ttctccaagg attacgctgg cagagaggtc gtggtgagtg gtcaagatat tttttaattc
    28681 agaggtcaaa tctgcactca ccgtaaacgc agtcgctgat agatccacat tctgttgcag
    28741 ggctgccccg ataccgttat tgagaggatc actctcccca aaaacctcat ctatcaggag
    28801 gatcttccct cgcagaacgt ccgtcgtttg ccagggctcc gcgtctggtt tatacaggag
    28861 agggattttc ttccccgcgt actcctgcaa atcacttagg tgagacgcaa tggatatgag
    28921 ccggttaccc tccgtgcccg caaagccaaa agatgtagtg tcagttccat agatcgtatc
    28981 cacttcaacg ccatcagcat tgtagatggt gagcgcctgg aggggcactc cctcgcgctg
    29041 gatggctact tcatagtttt caagcgtttg tataatttcc gcaactgttt ttgagggagc
    29101 gctgccggag agcgcttccc cactttcccc gctgatattc tcgatggtgg tcatcagcgc
    29161 tgcaccatca attccaaact gatcgtaaag cttttcctca gcgggcgtgc tcctcgccca
    29221 gctctgcggt atcagcgagc ctctgcccat ccaaaagatg atccctgcta ataccaccag
    29281 cagcagggtc atcaggagca gagggttcgt tttctttccg gttccctcac cattcacttt
    29341 atagcgtaga aacaggcgtc gggggatcct gcgagccggc tgcacggcga tgatgattcc
    29401 gatgagcaaa gaccccaaca agctggcaac aaaaatgagg agcatattgg tcgtgaaagt
    29461 ctttgaaaac agccagaaga tcagcgttcc cagcgcgctc ccgacgattc cccccatccc
    29521 gctgagaaaa acgctctcga gaacgatcca cccggagata tgtcgcttct tccaacctgc
    29581 ggcatgtaag aatttgaatt cgtcctctcg ttctcgtatg cccagcagca agttttcata
    29641 catcaccagc atgcccatca ccagagcaat ccccacgatc acaaaatgat agttgcgcaa
    29701 gcgcagcgcg atgaattgcc ccaacaaagt gacttgcagg atgccctgca gatttaacag
    29761 gatatttacg accaaattga gcaacgcaat gccggcggaa aatgtcaagg acgtgaacac
    29821 attgcgattc ttgcgccgcg agaattgatt gaaaaagaaa cgggaaaaag tgagtttttt
    29881 tcctggtcgt ttttccgttt gaggttgatg ggtgaattca cccctctgaa gcagttcaca
    29941 aggcgtcttc tgtaccaccc gccgaatcgc agggatgcca gaaactgtat aaagcagggg
    30001 aactgccagc gcagtgccga tgattgggaa cggagacggg tccatttcca gcgcgcttgt
    30061 gagcagcagc gaaaatcccg cggcaaagat agcccccacc aaaccaattg cgatcaattc
    30121 tgacaacagg tatctgaaca catctgcttt tctccaaccg accgagcgca gaacaccgat
    30181 ttcctcaatt ctggacatga gggatacatt ggtggtgaac atgatgaaca cgccagatac
    30241 gattaggaag gcgagcagca aagcgatgtt catcgcattg atgccctggg tgatgtgctg
    30301 cacggcgccc agagtggtcc atgcttcttc cagatacccc ataccgggaa cgtacaccca
    30361 cacattgcgc ggagaagagc cagcgatgac atccacatgc agtcccgtgc gctcgctgat
    30421 ctcactcgcc acccgttcca ccttttgcag gttttcttcg gaatattcct caatcccgct
    30481 cacgcgcaca cgaatggcat cgatataatc ctcccgcccg cttaaataac gggctccctg
    30541 cagattggtc agcgccagcg gtgggcgcgt caccaccgcc cctgggttca gccctggcaa
    30601 gatggtttgc gcagcgagcg gttgtgcaga ttcatcatat tgcaactccg ccatgggagg
    30661 atcatagatg cccagagggg catacgttag ttcatcaatg ctgtcatgaa taacgctgag
    30721 atcataaatg cccttcacat caaacatgaa atctttctgc tgaacaacct gcagcgggcg
    30781 gtagagcatc gtatcgtctg gaataagcgg cagattttca taatacctcc cactccaccc
    30841 ctcgcgcgac acctcaagtt cagaccccag catctccccc cagctcccat acgcttcaag
    30901 cttgaacaac ggttgtccat cggaaaacgg ggattgcgtc tgtgcctcat aacgatagct
    30961 gcccggtaat aacgcaatat cccgatcgat gttaacacca ccgccggttg tatacgcact
    31021 gccgggcaga tcgctgctga tctcaggagc gctgctcgtg ttaaagacca ccgattggat
    31081 cgccaagggt ttccgcccca cgctcagatc aagctgctca tcgaccagga gctgtccttc
    31141 tgcatccagg cgggcatcat aaatttgccg caggctggtt tgccacgctt cccctccttc
    31201 tgtctgaatg atcttcagcg tttctgtgct gccttccagc gggagagcct cgatcttcat
    31261 cgaaatgccc agctcagtat agttttgatt gttgatgatg aggggaacct tgttcttctt
    31321 tgtcgttctg ctttccaagg cctcctccag cggagcggct tccaggtaat cccctgcgag
    31381 aagtgccgta tccaagccga tcaactgcgc ttcctgctct ggatcgatcc ccgccaccag
    31441 cacccactgc gaggggatga tacctatatt catcaacgcc cattgctgtg caggagacat
    31501 agtcagatcg atagccagat tgccctgcat gaaatagttc ttgaagtaat cggtggtcgt
    31561 gaagtaggcg atctgtgctt cttcattttg cagggtgggg tcatcccctg accaggtgaa
    31621 ggtaaagcga tacagggtat tcacctcgcc cggatccagg cgcacaaaga cagcaccggt
    31681 agaattcacg aaatagccga ccgtggcgac cggagctgcc acttctacct catcgatacc
    31741 cttgatcaag gcgtattgtt catccgtaat tcccccataa gagctcccca gataatttcc
    31801 ttctaccaac tgggtgtccc gctccacgtc agagacggaa gatgcgggtc tgaccaggat
    31861 atcatacttg ccccgccagt gctccgcaac ctcggcggtg gaggtgacct cgaccgtatg
    31921 cagcatggca gaaaacaggc tgatgccgca gcaaatgatg aacactcccg ccacctgcaa
    31981 ccaggagagc ttcagtattt gcttgaattt gaaatggttg gtactgtttt tgaacttcat
    32041 caagttgctt cacaatgaga ggacaacttc tttcaaaccc aatacattca acaggagata
    32101 gtttgattct tcaatatgaa tcacctatcc gtttcacaga aaacagttcc ttttgctact
    32161 tatacgcctt ttttagatcg tttatttgta aacaaccgag gaatcataaa caggggcgcc
    32221 aggtccatta tacaatgtcc aggtttgata aaatgggctg ctacccccac aacggaattg
    32281 atggtaataa taatacgttc ctgtagtata tccacagctt gatgcacatc cacagggatc
    32341 accccagcag gcttgtatac aataatttgt cccttcccca aacaccccca cttcccacac
    32401 tccttgataa ttaggtgaga ataccgtttg ggaataagga tctgcattga tttctgcagg
    32461 aataaccagg ggttgaggta gctctggctg atccgcatag acgggttgaa cccctaaagc
    32521 aaggaacaat agtgtacata caagcaggca tacggcagtc tttttcattt ttccctcctc
    32581 gtcaaccgtg aacacgattg aacaaaaaaa ctttgcgtca gtctcatttt aaatatccag
    32641 aggggaaaaa caagtaacga agatcacaat acctaaattc aagatataca gccgcggcta
    32701 ccttgttgat gttctgccca cccggtcctg aggagtgaag aagtgtattt cattctcatc
    32761 gagctcaacc gatgcatcga ttttggtcat agagctggta tatcaggcag tgatttattc
    32821 ttttaaaaaa gcaacaattt ttaaagcgaa cttcccttta ctcctgtata atgaataaca
    32881 gaaaaacacg aatcaaagaa cattaatatt ttggaggtag cgaaaaatgg cagcaaaatt
    32941 tgagttgaaa gaaagcaaag ggcaattcat tttcaatttg aaagctccca acgggcgcat
    33001 cattctcacc agcgaacgct acactagcaa atctggcgct atgaatggca ttgagtcggt
    33061 tcgaaaaaac ggcaaagatg atgcccaatt cgaacgcctg gaatcaaaaa ggggtgaacc
    33121 ttattttgta ctgaaagcca ggaataacga acccatcggt catagcgaaa tgtattcctc
    33181 caaatctgct atggaaaacg gtatcgcatc ggtgaaagaa aacgctccaa tagccgaaat
    33241 cgaggatttg acagaataaa gggacgtgcc ctttcaattg aataactttt aacaaaaacg
    33301 cacatcctgc gcttctattg ggtatgtgcg tttttcattc tggcgcgcaa ttgtgcaaat
    33361 ttttcccgaa atcatgcgag gattataata aaacccaaat atttaggagt tgttatggcc
    33421 atttttgttg attctgccat ccttgaagaa gtgcagcagg ctgccagttg gggctggatc
    33481 gccggtgcca ccaccaaccc gagcttgctg gcaaaaagtc cgctgccgcc gcgcgaaacc
    33541 ctgcaaagaa tggcggaaat tttggatggt tatatcttct accaacttac atcccccaca
    33601 ctggaaggga tgcaacacga agcgctcgag gcacaccgca tcctgcaaga tcaattgatt
    33661 ctcaagatac ctgccaccac catcggtttt caggcagccg cgcggctatc ggatgattac
    33721 acttgtgcca tcaccgccat tttctccccc gaacaagctc tgctggcaca agcctgcggc
    33781 gctcgctacg ccatctatta ccacaaccgt gccaaacaat ggatgcctga tggcgctgaa
    33841 ttaggtccaa aaatggtccg ccttctcagc ggctgcgacg gtattgaagt gctcgccgcc
    33901 agtttcaaga acaaagagga ggtttttgaa gttgccggcg ccggcgtaaa acacctcacc
    33961 attaaacttg acctgctgaa gactctggca gataatgaat tctcccaaaa agcggtggat
    34021 gatttcaacg ccaccggatg tggtatcgtt taacgatcat tttggacaga cgccaaacaa
    34081 gagcctggag ggatcatgaa atttctcatc accggaggtg ccgggtacat cggcagcact
    34141 atttgttctg ccctggaaga taacgggcat acccccatca ttctcgattc gctggtcacc
    34201 ggttgccctg aattcaccaa aggacgcatc ttctacaaag gcgatattgc cgatgcagca
    34261 ctggtgcaag gaatctttga ggatcacgcc gatattcaag ccaccattca ctgcgctgcg
    34321 ttgattgtgg tgcctgaatc gacccagaaa ccttacgagt actaccgcga aaatgtcgag
    34381 aaatctctgg agcttttcca ttccctttat ctggcaggct gcagacgggt ggtgttcagt
    34441 tcctcggctg ccatctatga caccgttccg ggtttcatgg ttaccgaaga ctctcccttg
    34501 aatcccacca atccttatgc tcgcacgaaa atggtgatgg agatggtatt gaaagatttt
    34561 tgccaggcgt acggcatccg cggcattgcc ttgcgctatt tcaaccccat cggagctgat
    34621 ccaaaaatgc gatccggatt acagcttcaa agccccacgc atgtgctggc aaaaatggta
    34681 gaggtccagc taggacgtca atcagagttc cttatcaccg gcaccgatta tcccacccgc
    34741 gatggcacag gcatccgcga ttatatccac atttgggatc tagcccgtgc gcacgtcctt
    34801 gcggtcactg atttcgatgg tgttttcgag cgggcgggca gctcaagcgg caattatctg
    34861 gtcatcaacc tcggctcagg caaaggtgta accgtccgcg aattggtcag cgctttcgaa
    34921 agcgttaccg gacaacccat caaaaaacgt gaaaccgccc gtcgcccggg cgattctgcc
    34981 gggtcattcg ccaacgccga tctggctgcc agattgttga actggaaaac ggagcttacg
    35041 atcgaacagg gaattgcgga tgcgctcaaa tggggagcaa tccgtgacca ggtgctgcat
    35101 tattcctagc gcgcagcgcg cttgatatgt tcgaagaaga tcatttccaa ccccacctcg
    35161 ggggtcgtac tgccgcgttt tacctcgcca tccatatcgt ataacgcccg gtaaatacgt
    35221 tccaattctt caaaggtgaa ccgtttgctc tgcgggatga gcttttggat cacaaattcg
    35281 ctctccccaa gctgggatgc aacgtttcca gcagggtttt cttgcagcga ttccttgacc
    35341 aacagcaata agcggaactg gcgcacgatc atggaaaata cccgtacata atcctgattg
    35401 gcaaacatga gtttcaaatg gtgcagcgct ttgggggcgt cacccaacgc gaaggcatcc
    35461 accatagcga acacgtcttc ttcggggatt gcgctgcaca gtacgtgcac atcttccaat
    35521 gtgatattac gcttgccatt gacataggtg cataactttt gaatttcctg cctgatgatc
    35581 agaatatcat cccctgtcat gcgagccagt ccttctgcag cacgagcctc aatcctccca
    35641 ccctcttctt ccgccatgcg catgatgcga gcggggaaat ctcggcgcgc tggtacgcga
    35701 aattctttga aaaaatatcc gtcatctttg cgcgatgccc attgcgacaa ccaggcgtgt
    35761 tccgcaaagg gcttccacat ccaatctctt cctttcttga tgaggttggt tggaaattcc
    35821 agcaccaaaa tcgttgacgg gggaacattt tcaacgatct gctgccagcg ttcgatctct
    35881 tttttatcac gaacacgctc gagagaatgg ctgtagatta ccaggcgttt ctcaacacca
    35941 aaagggaaaa gcccaatgtt ttttattaat tcatccagcg aatctgttcg cccgttcaga
    36001 cgagcaacat tcaacgctgc catgtctgca tctcccacat cgcggatcat cgcctgtttt
    36061 actatctgca gggcatccag gtcatcaccg ataaagaaat aaacggaaca tcctttcttg
    36121 atctcgtagc gttccaactc ctgcattgct tcaatccgcc ttgaattccg tggtcacgcg
    36181 atggatcacc aatccgtatc gttgatagca gcgcacctct cgcaccagca tggtctgcat
    36241 atggtcagtg gacaccgctt tttcctgcaa aaatggtcct gctggtctgg ctgcttcaaa
    36301 gccgagccat gccagcagaa ctgccagcgg gatgcgcatg gctttcttgc gcaaattctt
    36361 acctgcgcca gccattgcca gccaggctaa cccgctcgac acagcaatac ttggcaccaa
    36421 attggaacca caatgatcgt gcattgccaa tccggtttct ccagcttgca tgcgctgcaa
    36481 ggcttcgtgc gcactttcga gcaccatccc gctcggcaca tcccccaaca gccagaatcc
    36541 ccccgaatca gataagccgc tcaccctggc gcgaacgccc tcggcttcca agacgtgcag
    36601 ggtagcgtgt tccaatgcat ggttcgagcg gattttccac accggattag cctccagaat
    36661 tagttccatt cctcacctct cttacactat tgattaaggg ctgattttac cgcgtctgtg
    36721 agcgaacggt tctcaataag ctcaatacca gtgggcaact tttccgactt tcgcaagcgt
    36781 tgcgggataa tggctttcgt aaaacccatt ttttcagctt cccttaaccg cgcaaccata
    36841 ttgctcaccc agcgcagctc ccctgataaa ccaacctctc cgatcagaac catttcagga
    36901 tgcacagctt tgtcaagtaa tgaagaagca atcgctgtcg cgatcgccag atcggcagcc
    36961 ggttcgtcca cattgaatcc gccaaccaca tttacgaaca catcctggtc aaacaatttg
    37021 agatgattac gccgtgtcag cactgctacc accagcagca gacggttgaa atcaacccca
    37081 ttggatgtgc gccgaggatt gccaaagttg gtagtgctgg tgagtccctg aatctcgatt
    37141 aacaacggac gtgttccttc catcgtcacc gccacggcag agccgggtgt attttccatc
    37201 cgctccgcaa ggaacacctc tgatgggttg gagacctcca ccatacccgc ttcgcgcatt
    37261 tcgaacaccc ccacctctga ggtgggaccg aacctgtttt tcatcgaacg caagatgcga
    37321 tagctctgaa atcggtcgcc ctccaattgc agcacagtat ccactatgtg ctccaacaca
    37381 cgtggaccgg caagcatacc ctctttggta acgtgcccga tcaaaaagac ggtcgtcgcg
    37441 ctgctcttgg caaattcccg caagcgcgaa gcacattcgc gcacctgggt caccgaaccg
    37501 gcgcttgagt tgaaaacctc catcgtcatg gtctggatag aatccacaat caggagctgt
    37561 ggtttaacgt tttgcgcatg ggtcaggata gaatccagat cagtctccat cagcaaatac
    37621 agattgggtg ggaaggttgt tgtttttcca tagttccctt ttgcctgcag gcgttgagcg
    37681 cgcattttga tctgctgccc tgattcctcg cctgacacat acagcacatt tcccttttgg
    37741 gcgaactcta tggcggtttg cagcaacagg gtcgattttc cgatacccgg gtctccgccc
    37801 accagcacga tcgaaccagg cacgataccc ccacccagca cgcgtgaaaa ttcttcatta
    37861 tccatcttcc agcgttctgc gctctcgccg ctgatctcat ccaaccgtaa gactctggag
    37921 gtggttatgc cggcagccga cctttttttg ccccctccag agggctgcac cacttcttcc
    37981 accatggagt cccattcccc acattgcggg cagcgcccca gcggtttcac cgaaacccta
    38041 ccgcagctct ggcatacata gcgtgtttct gttttggtca taatgatcaa cctaagtata
    38101 aaacgaaaaa agggaagatg gtggctcatc ttccctttgc gtggagtgaa acgaactacg
    38161 cacccgcttt caggatttct cctcgatcgg gtatgcgttc gtcgtctcta aggagtataa
    38221 aatcgccttc ctggtcaacg tccagcagaa tatcatcccc atcgcggaac gtctcagcca
    38281 gaagggcatc ggataattta tcttcaatct caagctggat tacgcgtcgg atcggtctgg
    38341 cacccatatc gggatcatag cccttttcag ccagtttatc cactgcagcc gggctggcgc
    38401 gcaggcggat ctctcgttcc gataggcgct tggcaacttt caccagttcc atctcagcga
    38461 tctggcggat gtgctctcga ttcaacggat ggaacacgat cacattatcc aaccgattga
    38521 gaaattcagg gcggaaggct tttttcaacg aatccaatag cttttcgcgc atctctttat
    38581 aaacgttcgc ttcctctttt ttcgtatcct gttccaaaac aaaacccagt cctgattggc
    38641 gcttgatcat ctcagctccg atattggaag tcatcacaat gatggcattg cgaaaattca
    38701 ctttacgccc gtgtgcgtcg gtgagttgcc cttcttccaa tatctgcaag agcgtattgt
    38761 gcacttcagg atgcgctttt tccacctcat caaaaaccac gatcgaatat ggtctgcggc
    38821 ggatggcttc ggtaagctgc cctgcatcct catatcccac atagccggga ggagagccca
    38881 tcaggcggct aacggtgtgt ttctccatga attccgacat atccaattga atgagcgctt
    38941 cttcgctgcc aaacatgata cgtgccagtg ctttggtcag ttcggtcttg ccgaccccgg
    39001 taggacccag gaacatgaac gatccaatgg gtcgtttagg gtctttcaat ccggctctcc
    39061 ctctgcgcac ggctttggca atcttttcga tagcctcatc ctgccctacg atcatctcgt
    39121 gcagttcttt ttccaggttc aacaatcgtt gtgtttcttc ttgggcaagc tgcatgatgg
    39181 gcacgccggt ccacatggaa accagttctg caatatcatc cgtacggacc acggggctgc
    39241 tgtcgcgatc ccactggtcg cggatggtgt tcagtttttc ttccaggtct ttgatcttgc
    39301 cggctacttc taacgccagt tcttcgtcac ctgcttccaa catctcggag tgtctggtgc
    39361 gtacctggcg cagctcatcg gtgatctgtt tggtcacctt ggcagcatcg cttttataca
    39421 tgcgaacacg cgaagccgct tcatcgatca gatcgatggc tttatcaggt aagaaacggt
    39481 cggtgatata gcgtgccgat aaattcgtgg ctgcttctaa cgcttcatca gagatcacca
    39541 gatgatggtg ctcttcgtag ttcttgcgaa cgccacgcaa tatttccaga gtttcttcct
    39601 tgctcggttc gttcaccacc accggttgga atcgccgctc caacgcagca tcactttcaa
    39661 tgttcttgcg gtattcatcc agggtggtag cgcctaccac ctgtaactcc ccgcgcgaaa
    39721 gcgccggttt caggatgttg gctgcatcca ccgaagaacc ggcagatccg gctcccacca
    39781 gcatatgaac ttcgtcaatg aaaaggatgg catcagacct cttcagttcg tcgattactc
    39841 tcttcaaacg ttcctcgaac tgcccgcgat acatggttcc ggctaccaga gagcccacat
    39901 ccaggcgcag cacacgtttc ccaagcagtg ggatgggaac attcccttcg acaatgcgtt
    39961 gagccaaccc ttctacaatg gcagtctttc caacaccggg ctcgccgatg agcgccgggt
    40021 tgttcttggt gcgccgtgca aggatctgga tcacccgttc tatttccttt tcacgcccaa
    40081 tgaccggatc aagtttatgc tcttccgcct gtgtggtaag atcaaccgcc agttgatcca
    40141 ccagcggcgt cttcctggga cggcgggatg atgttggagt ttcttccgga acttcagaaa
    40201 ctttcgaaag aggcgcctgt ttttgattga tctcgcgcgt gatctgccgc cgcacctgct
    40261 caaaagtaac ccctagttta ctcaacacgt tttcgcacaa tgcatcatta gaatttacta
    40321 tcgccaacag caagtgctca gtgctgatgt agcggctgct cattttttga gcctcttcca
    40381 aagaaagcgc gaccactttc tgcgcatcca cagaaagatc gatccgctct ttattgattg
    40441 tacccatgcc ggtcaggcgc agcaccattt cacgtgcgcg ctccagctca attcctaatt
    40501 cacgcaatac ccgccctgct actccgttgt tctcctggat caatcccaaa agaatatgct
    40561 ctgggtgaat ctgcggatgg tgaaactgct ctgcctcttg atgagccaga ctcagcacct
    40621 gccgcgaact ctgtgtgaat ttttccaaac cagacaatga acgctcctta atgttcgatg
    40681 gatcagatct tttttatcta ccataccatc tttgtacctg gattgtacca agaaagcatt
    40741 agaaattggt cagaatgatt gttagtttat tgggttgctg gaaattgcag aaccgctggt
    40801 tgtttacaat ccttcgaatt ccgtgatctt tttgcgccaa ttttcaggaa ccgggatggt
    40861 tgttttgctg tgaaaatcgt aggttaccat gatgatttcc gccagcgctt tcagagatcc
    40921 gtcttttcta cccctgatct cgttttccat gctcatgctt ttcttgccga tgcgcgaaac
    40981 ctttaacgcc acctgaatct catcctccaa ttctatgggt gctttatagg cgatatggac
    41041 atccgcgacg atcaatccca ggtcgagaaa ggattttcca tcccacaacc ccagttgccg
    41101 gacatacgca agccgcgctt gttccaaaat ggtgagaaaa cgagcgttat ttacatgcca
    41161 ctgagcatcc agatctgcat agcggatctg taccgggtag aaaaatcgaa aatcagacac
    41221 gccatgatta taccggaaac acttgcaaag ctgcatttct ttgttagact tataaaatgg
    41281 acaatttgta gacaaattat ccaaagagga agataagaaa tgcagataac acgtcaagca
    41341 gattacgctc tcagaacgat catttatcta tccaggttgg gggaggatga aaaagcgtcc
    41401 accagcaaca tcgccgaaaa acagcatatt ccatcctcat ttttagccaa aatcatttca
    41461 cagttgtccg tcgccggcat cattcatacc tccaggggtg cccatggggg agtctctctg
    41521 gcaaaaagcc cggataagat ctctgtgctg gatgtgatcg aagcgatcga cggtccggtg
    41581 atgttgaacg aatgtacgca caatcccggc atttgcatct ttaccaaaga ttgccctatg
    41641 catgttatgt ggtgtgacac tcgcaaagaa ctggtgaaca aattagaaac gatcacgttc
    41701 gaacagtttt ccagcagcaa gtatgctgtt ctttaacccc catttgcgta agctttcgtt
    41761 caggaatccg gtttcataaa aaccatgatc tgattctcat caaattggat acggtagttt
    41821 tgttccaaat ccgcaagcac ttcagggaac ccctcttcca aatactgcca gtcatccacc
    41881 gagagaatga tgaaatcgta acgatcagcc aggatctgtt cagggatatc ctcctgggta
    41941 taggataagg tcagattatc tcgttgaatt cgctggttga acaagatgtt gtacatcccc
    42001 aaaatatcat agggattgtt gctgaacata tggtcttcat tttccagggt ttcctcaaag
    42061 atcgtggtag tggtatacat tttcacgtca tcgctgacca caatttcatc ttggaactcc
    42121 aggaaaggat aatatttgat ctggaacttg gcattggtag tcggttcatg atagatgtat
    42181 ttgtaattgt acgagatttg tggcagaagc catgaaacca ggaagaagag aaggatgcca
    42241 actgtgagca gattctgttt ttctaccaga atcaccaaac ccaaaaatac cgaaagcaaa
    42301 atggggaagt agatgctttc aagaaatttt ccataattcc agtctatttt ttccacatac
    42361 tgcatgccga gctgacgcat gaccaccacc agcagaaagg cggttagtat gaccgccatg
    42421 aacttcagat aaccttgcct gtcgtgcggc gtatcgaaag agatgaattg cggggcaaga
    42481 atggcgatgg ctggcaatgc cggaaagtaa aaccgttcga tgaagcccca gggaatcttg
    42541 agcatgttca acgtgatgaa actcaccaac aataacggga atgcccatac aatcttcttt
    42601 tggggtgtga tggctcgtgt gctgagtttg acccctccca ccaaaaagag caggaaaggt
    42661 atcataaggt cgtcaagcag gtaaattcga taccaattcg ccggttccac tcgaaaaccg
    42721 cccgtgtatg cataattttc gaacacttct ttcaaagtgt cggggctgat agcaaaaaat
    42781 ggttgcccca aaaagatcgc atccaggatc ataaagaacc cgaccgcccc cagaagacca
    42841 agaaagtagt tttttaaata aggtgaaaca ttttttagag aaaattttcc ctgcctgtca
    42901 aagaagaaac cgagcaaaat cacattggag aacagggtgg tttctttggt cttgagagcc
    42961 aaaaaggtca gtgcgcccag ggcatacaac agccatgctt tttccgttct ctggtaaagg
    43021 atataggtga ataccagtgc cgttgccatc atcatggcag taatatctac cgaagttaca
    43081 ccggcatagg aagagatgaa tttatacgaa agaaagagcg caaccgccag caaaccatgc
    43141 atggctgtgc tttctttgag aaagatgcgg gtgctccagt agatcagcaa agcggtcaac
    43201 gcgatcacga aagcccaata tatgcgcaca ccgatcagcg gggtgggtgc aaccgccatg
    43261 aaaacttttt gcaagtacac atgaaaatat cggttcccat aatttggggc gcgctcgttg
    43321 ttcaacccga tctcaatgta gagcagctca tccgaataga tcggtccgcc agtgttgaaa
    43381 gaaagtccaa cccagagggc aataaaaaac aggacggata gagcaatttc gatggttttc
    43441 cgtttcattc catttcctcg gtattttcaa aatacagtaa caattctttg attttactat
    43501 acccgctatg ccttattttt tctttcctct tataattgac ctaccacaaa gaggaatgag
    43561 aagatatgct gattcattct gcatcacaat tgatctgtct gcgcggcggt ccacagcgag
    43621 gagaacaact gggtagcctg gatctcatcg aagatggtgc ggttctcatg catggtgaaa
    43681 cgattcgaga ggtcggaagc acaaaagatc ttttgcagcg cttcccgcag gaagaacaat
    43741 ttgacgctct gcatcgggtg gtcatgcccg gttttgtgga tccccatacc catctcgtct
    43801 gggcgggcga tcgcgctttg gaattcagct tgcgccttga gggaaaaagc tacctggaaa
    43861 tcatggcagc gggtgggggc attctttcca ccgttaaagc cactcgccag gcatccacca
    43921 gccaaatcga aagggaagcc atccggcgcg cccgttccat gctgcatcac ggcacgacta
    43981 cccttgaagc caaatccggc tacgcgctgg atttgcatgg cgagttgcgc ctgctcgaga
    44041 ttatcatgaa cctgcgcaaa agcttgccgc tagacattgc ccccacttat ctgggagcac
    44101 acgctgttcc ggaagaattc aagggaaacg tggctggcta caccgaactc ctggtaaagg
    44161 aagctcttcc ccaattaaaa gaatggtggc tgctgcatta ccctgatgaa aagctgccat
    44221 tcgttgacgt tttctgcgag ccatcggtct tcaacctgga gcatagcaga gctattcttg
    44281 ccactgcgcg cgagttgggg tttccgctca aaattcacgc ggatgaattt gagaatatcg
    44341 gcggagcttc gctagccacc gagttaggag caacttcggc tgaccatctc gtcaaaacca
    44401 gccctgaaga tatccaaaaa ctggcttcca gcaacacggt ggcggtagcc ctgccctgca
    44461 cccctttcgg tctggcagaa agcagctaca cgccagcccg gtcgatcatc gcacagggcg
    44521 gtttgctggc tctggcgtca gatctcaacc cgggcaccgc ctggtgcggc aatatgcagt
    44581 tcgtcatcgc cctcgcctgc cgtttcatga aattgacccc tgcccaggct cttgcggctg
    44641 caactatcaa tgctgcggct gcaattaaat tggagcacag ggttggctcc ctggaacccg
    44701 gcaaacaggc agatttgctt gttttgaaca tccccgatta tcgccatctc ggttaccgct
    44761 ttggtgaaaa tttggttgcg accgtcatca aaaagggaaa ggttgttgac ttctcatgag
    44821 tcaccgtgca ctcttggatc tgcctgccgt gcgagactta tatccgcaaa ttgatcctgt
    44881 gcacggcttt gaccacatcg aacgcgttta ccggctatgt caggttattg gtgaggcaga
    44941 aggggcggac atggagattc tgctcactgc cgccctgctg catgactgcg agggcagcga
    45001 ccccacctcg caaagtcgcg cggagcatca tctgctttca gccgataaag ctggcgcaat
    45061 cttaagagaa ttgagatggg cagaggatga cattcacaaa gtacaacact gcattcgcgc
    45121 tcaccgtttt cgcaagagcg aggaaccgca ttccatcgaa gctcaggcgc tgttcgatgc
    45181 cgacaagttg gatgtgatcg gtgcaattgg catcgcccgt accattgggt acgccgtgca
    45241 ggcaggcgaa ccaatctatt ccagaccgtc tgagcaattc cttgccaccg gcgcgctcgc
    45301 agagggtgaa caccacagtg tattccatga atacctgttt aaactgagaa atgtgcacac
    45361 caccctctac acagaaacag ggtgcaaact agccctgggg cgccatgcat ttctcgatat
    45421 gttcttccaa cagctcgaag aggaagtgaa cggaacccgt taatttttta gccagattgg
    45481 agctttttac tcaatggtac ctgatcttaa ttatctgatg cgcattgcca ggcaggcagg
    45541 gcaaatttta atggagggct atctaaaaca acacgagata cggcacaagg ggcgtattga
    45601 cctggttacg gaaatggaca gtcgttctga agaatatctg ctgagtgaga ttcgcaggga
    45661 ctttaaagat cacaccatcg tcactgaaga aagtgggctt attgaagggg ttcaagagca
    45721 ctgctggttc atagatccgt tggatggcac atccaattac gcgcacgggg ttcctgtttt
    45781 cagcgtcacc attgcctatg catttcatgg cgccatcaca ttgggcgtca cggtggaccc
    45841 cacccgcaac cactgtttca gcgctgaaag aggacagagt gcgtttctca atggacagcc
    45901 cttgcacgta tccgctaccc gagagctggt ggatgccatg ctggcaacag gttttcctgc
    45961 tgacctgcat gacccggcaa agaacaatct ggagaacttt tcccgcttcc tcatgagcgc
    46021 ccagtccatc cgccggttag gctcggcagc ccttgacatc gcctatgttg ccgcgggttg
    46081 gttagacggc tactgggaga ttggcattca cccctgggat attgcagcag gaacgttgct
    46141 gatacaagaa gccgctggtc tggtcaccga tgtacaggga gaggctgatt ttttcaagcc
    46201 gccatattcg ttgatcgccg ccaatccaca cctgcacgcc aaaatgctcg caatgctcca
    46261 aatggctgat acccgataaa aacccatgtt gtataatggg tctgttatgt accatttcat
    46321 ccagaggatt ttctatgact acacaaaatg atgttttcaa acaatctcgt tggagcctga
    46381 acgacctttt cccatccgca aattcaccgg aaatgaacaa tgcgttcaaa caggctgaaa
    46441 aaatggtcgc cgatttcgaa aacaatcgcg aaattctatc agaagacatt cccgccgaca
    46501 ccttcttgca gattgtcaaa gacctcgaag cgcttactcg ccagatgcgg ctgatcaacg
    46561 attatgccga gttatggttc acggaagaca cccaaaacca gcaagcgcaa agtttggctg
    46621 cccgcgcgga gcagttcgtg actgaacaat ccaataaaat ccttttcttc actttgtggt
    46681 ggaaagacct gagtgaaaag aacgccttgc gactgatgga tgtttcgggc gactaccgtt
    46741 actggctgga ggaaatgcgc cattttaagc catataccct cagtgaagcg gaagagaaga
    46801 tcatcaacac caaagacctc acagggttcg ctgcgctcat caggttgtat gattcaatca
    46861 ccaaccgcta tacctttcgt gtagcggtag atggggaaga aaaggaactc acccgcggag
    46921 aattgatgtc gctggtgcga ttgcctgacg ccaacctgcg cgccgccgct tatcagcagc
    46981 agtatcaaaa gtacggcgag gaaggacctg ttttagggca gatataccaa accatcgtcc
    47041 gtgattggta caatgagaat attggcttgc gcaaatacaa aacccccatt tccgccagaa
    47101 acctcggcaa cgacattcct gatgatgtcg ttgatacatt gctggatgtt tgtaaaaaga
    47161 acacatcggt attccagcgc tttttccgcc tgaaagctaa atggctgggt gtagacacca
    47221 tccgccggta cgatgtgtac gctccggttg ctgaatcaaa taagaaatat ccttttgaaa
    47281 tggctactga actggttttg gattcgttcc aagaatttga tcctcgtttc gcgcagttag
    47341 cgcagcgcgt gttcgcccag gcacacattg acagtgaagt gcgcaaggga aaacgctttg
    47401 gcgccttttg cgccacggtt gcccctgatc tcactccctg ggtgctgatc aattatcaag
    47461 gcaaagcaga agatatggca acccttgctc atgaattggg gcatgcgatc cattctctgc
    47521 tggcttcaga tcacagcatc tttacccagc atgcttgcct gccactagct gaaaccgcat
    47581 caactttcgg agagatgatg ttgatcgacc gcttgctcgc tgaagaaaaa gatgaaggtg
    47641 tgcgccgttt cttccttttc aaacaggtcg atgattccta tgccaccatt atgcgccagg
    47701 cttatttcgc tttgttcgag aagaccgccc atgagatgac tttgcaggga gctgctgtgg
    47761 atgccattgc cgacgcgtat ttcgaaaatc tacaggacca attcggcgat tccctcatcc
    47821 tcggcgaaga attccgctgg gaatgggttt ccattccgca tatctaccat acccccttct
    47881 acgtgtatgc atacgctttt gggcaattgc tggtcttatc gctctacaat caattcaaga
    47941 acgaaggtaa cagtttcaaa ccaaaatata tccacctgtt atccacaggt ggctcaaaag
    48001 ctcccatgca gatattggat gaggctggaa tggatgttcg gaagccagaa ttttggcagg
    48061 gcgggtttga cctgatcgct acgatggttg cccagctcga aacgctctaa ggcgtgtgtt
    48121 tgaaaaaaga tgccggcaat tgccggcatc ttttttaatc gtttacctct tcttccgatt
    48181 cttccacttc ttcaagcacc gtctcttttg cttttcgatc aagcatctgt atggcgccca
    48241 acactacggt cgtaaagtac cgcgtggttc cttcgtgctc gtattttcgg gttcgcaacc
    48301 ttccttccaa atagaccagc ttcccttttt cgagatattt ctggcatatc tctcccagct
    48361 tgccccatgc ttctatgttg aaccaatctg cctcttggtg cagttcatcc tttttgtctt
    48421 tccactttcg gtcgaccgct aaagaaaaag tggtgtattc ggtgcctttt ggggtcttgc
    48481 gggattccgg atctttgccc agcctcccaa tcaattgaac acgattcaat gatgccatgg
    48541 tatccctcca tttttcgtat ttcaatgtta tataatagtt tagattaatc tatcccattg
    48601 tcaacaagtt ttgaggagcg agcatgtaca ttgccataga aggagtgatc ggtgtaggga
    48661 aaaccagcct tgcccgcctt ctgcaaccat catacaaatc aaacctgctg cttgaaatat
    48721 tcgaagagaa cccctttctt caagagtttt accaggaccg cgcacgctat gcgttccaaa
    48781 cccagatctt tttcttgctc agccgctatc atcagcagca caataacgtt cctgaactgc
    48841 tgcaaagcca aaagaacctg atatctgatt acacttttga aaaagacgct ctgttcgcag
    48901 gtgtcaatct gaaaggggat gagttggatg tgtatcattc ggtgcatact gcgctggctg
    48961 agagaatccc ggcaccggat ctcaccgttt acttaaaagc caccacagat acgctgatgc
    49021 agcgcatcac catgcgagac cgttcttatg aacgctctat ggaaccagat tatattgcca
    49081 cgctgaacga agcgtatgaa aggcatttta ccagcgcggg aaaggaaggg agccctgttc
    49141 tggtaattga cagcaatgac ctggatttcg ttcataatcc cgccgacctg cagttcatcg
    49201 acgaccggat tcggcaaact ttgcgtttac cacctttcca ggttgcgctt cctatggatg
    49261 gataatgaaa aaaggatagt gaaatgagag ttacaaggaa catgcttctg gaaaccgctc
    49321 gcaattttgt gaaggaaaaa accttcaacg accccgacgt cgcttgcgtt tttctggtcg
    49381 gttccttggt tggagaagac ccgtttctag gtgaatccac agatatagat ctgattttca
    49441 ttcacaatac cgagcctgca ctgagcagag aggtgagatc gctcggtgac gattttcaat
    49501 tggatatctt tcattatccg cgacgtgttt ttaaccagcc caaagaactg cgccaggacc
    49561 cctggttagg gtgcctgttt tgtgagactc cctttgtttt gtatgacaca taccattatt
    49621 atgattatct acgggcgggc atcttttcta acttcttttc accagtcaat agctatgctc
    49681 gctctcaatt cttttatacc aacgcgcgca accgctggat gaatctccag catacggcgg
    49741 aagagttctc tgcggatgtc gtgtatgact atctcttgac cctctatgat gctggtaatt
    49801 gtatttcttg cctcaccggc agaccacttg ccgagagaag attcttgagt gagttggaat
    49861 tctgtactaa ctatttggga cgtcccgggc tggcaagcgg catgagagac ctgtatggca
    49921 tcaatgacta ctccgcattt ccatgggaaa cgtttttgga acaaatgaag aatgctatcc
    49981 ttgccctttc tgaaaaatca tattgccctc cacaattctc atccgtacgt ttcagatatt
    50041 atcagcaggc aatttcacac taccaggctg agcgtcccga tgaagccctg tggatactat
    50101 tttgggtttg gacatctgtt atacacacgc ttcctaaacg cgcatcagaa gcgaaagtct
    50161 ggaaaaactt ttgtgaacaa cttgatttca cgcgcgatat ctttgttcag cggttgcaaa
    50221 atttagatag ttacctcgag gcagtgggca caacctgcac ggaatggcag agggtcagcg
    50281 gattacttta attttccaca tttttttcca caatgctgtg gaaaaccccg aaatatctgt
    50341 ggataagttt aactatttgt ggatatcttc gaataaaaat tctactaacg gtaatggttt
    50401 tgttgatatc tttcggaatc gcatctgaaa agttgttgac aattcagaga tggaacatat
    50461 ttaacttttt tcgttgaatt tatgccatac ttattcacaa acatattatg tgtgagagcg
    50521 gtgaaaaaga cctatatttc ctttcttttt gaacgcctat actgtatatg gggatgatct
    50581 cgcttttatg aacatattca cagagcctac tactacaacg aatactttaa ttaaataact
    50641 aaataattga ataaacccaa aattaagcat gcgcaaacat gcttgtatat gcactccctt
    50701 ttttgtacaa tgcttttaca aataaaaaaa aagaattgct ccctttggca agggaaaagg
    50761 aaaagacttt tttctaaaag gaagggaaac catggacgta atcaaagtaa aagctgtttc
    50821 ccgcacagca gcagtcgcgg gagcgatcgc gggagtaatt cgagagcaca agcatgctga
    50881 agtgcaagcg atcggagcag gagctgtgaa ccaagccatc aaagccctca tcctggcaaa
    50941 gaatttttta gaccaggatg gcattacagt ggttttcatc cctgaatttg tcaatgtgga
    51001 gatagacgga aaagtgcgaa cggctatcaa atttattata gagcctcgtt aaaggaaaga
    51061 ttaaattaag aacactgaaa tagaattact cctattctat ttcagtgttt aatattgatt
    51121 gtattcttcc ggcaaaaaaa caacctcttc ttttccggca attttttctt gtattttttc
    51181 taaaataaga tccatatgcc gggtgtgatt tacaaaatcc aggttgtctg agggtacggt
    51241 gagcaccggg caaagggtga aattctgtat ccaggtctca taggcgtcgt tcaatgtttg
    51301 taaataatcg gtgctgatat ttttctcata atttctatta cgacgacgga tgcgctgttc
    51361 aagtgtattt acggatgccc gtaagtagac caccagatct gggataggca gaaaatgtag
    51421 cagtgtctgg tagatggttt cataggtctc atgatcgcgc tggttcataa aaccttgcag
    51481 atacagattt cgcgcaaata tctctgcatc ttcatataga ctgcgatcaa gaatgaccga
    51541 gttagggcaa tccaggagtt gttggtagac cttcaaccgg ttcgaaagga aataaatttg
    51601 ggagtgaaaa ctccaggtgt acatatcagc ataaaaatcg gaaaggtagg ggttattagc
    51661 ctcaggttcg aaaaaggggt tccaatcgag cctctggctg agcaggcata ccaatgatga
    51721 ttttcctgct ccgatgtttc cagcgattgt gatgaatttt ttcatgatta aaacaattat
    51781 agatgggaac agacataaaa aaagagctgc atcttctgca actctttttt ttaagtttaa
    51841 aaagggttag ctttctttga tgatagcatc aaccgggcat acttctacgc aggctccatc
    51901 atcgatacac tttgccgggt caatttcaaa gtgttcctca cccatagaaa tggcatcttg
    51961 cgggcattcc gcttcgcagg aaccacaaac aatgcattca tcagtaatta cgtgcgtcat
    52021 cttccaatcc tttcgagaat gtattttata cgttctagcc acttaggata gcaccataat
    52081 tttgctttgt caacgaatcg aaatagtgat attttcagcg caaagtaagg ttctattctt
    52141 caatttcctt gatccgctgg cggaaccata atgccgttcc gggttgataa cggcggacaa
    52201 taagaataga gcatgcgact tcttctgcca gcgcatcgtt aagttttccg aagatgtaat
    52261 ggggcgaaaa cacctcttcg gatgcaccga tgatcatcag gtcgtaagat ttcttttcag
    52321 cctcgcttac gatcccttcc agaacatcgt tcgacgtcac gacgcgagtt tctaaatagg
    52381 ggggaacttc tcccaattca ttttcaatga tctcctgtgc ttgcagcatt tcgtcttctt
    52441 gagtttcttg attggcatgt tttggaatca cccgcaaagc ggttattttc gtgtcgtggt
    52501 ggaaggcaag gtcagaggct attttgatcg ccaggtgggc gttgggtccg ttgccgactg
    52561 gaacaaggat gttctctagc ccgttcattc ctgaatcgcg cagaacgcct accttctcgt
    52621 gtgccatcac cagaacttcc ttgatgatgt tgtggggcat catgatcttg gatttcgtcg
    52681 atggccatcc catgagaacc atttgtaccg ggtttggtga tttcaattcg ttgaaaattc
    52741 cagtctctac attgggtgct aatttgtgtt ttgtatagat gggcacattc cttgcttgtg
    52801 cgatgggagc agtggcgttg aggagatcgc tctcgatctt cttagttttc tcgatcagat
    52861 cctgaatgat ttcttgcgat gcagtgcgcg gtgccttcac cacgttgaac aggcagatac
    52921 tggtatcttc ctggtgttca gccaggcggg atgccaactt caccaatcct actgccgttt
    52981 tcggattaac tgtgggcacc agaatgcgcc caccacctac ctgctcttcg accttttcat
    53041 gcttttccac taacttgttg atccatttcg caatgtagta aattaccgaa aggatgaaaa
    53101 gcaccaagcc gagaaattga agggctgctt tgctggcgaa cgtaatgatc aaaacacatg
    53161 aggcagccgc cagacaggca gttaggggaa agaaaggaac cttgaaaggt cgtttgatat
    53221 cagggtgcat tttccgcagc cggaacattg ccagggttgc ccaaaacaaa acgaacatat
    53281 aaccggcgct cgaaatgtag cttaagaggt caaccacctg tagcagcgct accaacccgc
    53341 tgacgatcat gatgaagata atcgcaacat atggcgttcg ccaatggctc aggcgggaaa
    53401 atatccgcgg ccatacgccg tcgcggctta aagtgaatgc ttcgcgggtg gcggagagca
    53461 tggctgaatt ggcagcggtg aatgtggcaa cgatgcccgc cagccccatg accgggacac
    53521 ctacttttgg ccagaaaaga ctggcagcgt cggtcaacgc agtttctgat cccgccaatg
    53581 tggtataggg gatcgttcca atggttacca gggaaaccag ggtgtagacc acggtggtga
    53641 tgcccaggct gatcagaatc cccaggggaa tattcttttg agggtgcatg acctcctctg
    53701 catcgtctgc gattacctca agacccacat aagcgttata cacaagggcg atggttgtca
    53761 acatgcgccc aatatttgca gccagcgact ggttttcaaa gagcaccctg ccggattgaa
    53821 aggtgtctac attaaaccca ttgggatggg tcaaaccgcg gacgatgtaa atggcgaaca
    53881 ctaccaacaa aaagccgccc aagacgatct gcaagtttcc ggcttgcttt accccgcgca
    53941 cgtgcattat ccctacgaga atgatcacac ccagcgccac cgggatgatc ggcaggttgg
    54001 ggatgaacac gctcaccgaa taagcaaagc cgatggaaga cagtgcggca tagaacgcac
    54061 tggaaagccc atcgagcgag ccgaccagga acatgatgag accctttcca aaaccttcac
    54121 caacataggt catggctccg cctgttaccg gataggtggt ggctagttca caatagttca
    54181 gcgcaacgct taacgttagg agaccgccga tcagcaaagc cagcaccgca tccggtccgg
    54241 caatgccggc ggcatgtccg gtcagcacaa agatctccgc cgcgatggtt ccggcggtgc
    54301 ccagcatgat aatctgcaaa agattcagtt ggcgtttcaa tttccgtttg ctcatagcag
    54361 ttcgcaacct tttaatagtc gaatggtcta ataatttttg tattttaata gtttggggaa
    54421 ttgatcaata aggaataagt tatcggagac gttttcatac agtgaatgac aaatatgtcc
    54481 tctattatac ccatatattt gaatataaaa aaataaaaaa tcacctcgaa cgattgaact
    54541 tgacaaccca cagtgagata cctataatta tcgcacgttc ctcgatagct caatcggcag
    54601 agcaatcggc tgttaaccga tgggttccag gttcgagtcc tggtcgagga gctttctttt
    54661 taaagaatct acgttgctct tgtcagcatg gtttctttcc tgtctttcgt tgctataatc
    54721 aaaaagaaga aagatattgg aggtatgcat gaaacactgg aaaatagcca ctttgcttgt
    54781 tttttcgctt gtttttttgc aggcatgtga tgttgccatt agtgttggag atgcggacgg
    54841 tagcaaagcc agcgttcccc tggcagtcaa cgtgcaggcg cctgctcagg gcagccaggt
    54901 tccaatgggc gccgtggata ttcgatatac cgcaaccgcc cctgagggag tggcggtggt
    54961 cgagttgagc attgacgggt atatcgtcaa ttcttacaca gcccccgacc cctcgcagaa
    55021 taccgttgcc ctggtgtata gttggacccc cccgaccgtt ggcagccata tcattcgcgt
    55081 gcgtgtgcaa gactctaaag gaacatggag cgattacacc gatgtgatgc tcaccgtcac
    55141 cgaagcccag cccgatcaat ctgcggcgga tcaaagctcc cagcaagcac aacctaccga
    55201 tgccccgctg cccacggcaa ctccatccac gccctatatc tctggggtta cccacgatgt
    55261 gaacaaattc tattatggca acagcacctg cggacccacc aagatcacca tctctgcgaa
    55321 ggtttctgac cccagtaaag tatggagcgt ggtcatcttt acccgtttca tggataagga
    55381 aggggagggg caatccaaat gggataccgg acatgccatg aatcccaagg gcagcagtgg
    55441 agaatattcc atcaccctcg actcttccaa gatcgctaat tttaacacct atgattacgc
    55501 ggtcttccgt taccagttcg ttgccaccga caagaaccgc aacgaggttg ctcgcaccga
    55561 cgtgtatgaa gatattgatt attctatctg cccctaacac tgaatgatga gggggatcga
    55621 tcagatagag aagcctgcct ttgatcaccg gcgggctttt tctttatgac catgttcatt
    55681 gactgccgtc acttgacgat gttttttcct gcactaaaat caaaccgatt ggtcggtttt
    55741 tatgcaacaa cgtagtgtag agacaaaaga aaaaatcttg cagacagcgc aagagctgtt
    55801 ttccacagcc gggtatgacg caaccggcgt ttctcagatc tgtgcggcat gcgggttgag
    55861 caagggcgct ttttatcatc attttccctc caagcatgct gtgttcatgc atcttttgag
    55921 aacctggctg gaacaactgg ataaagaatt cttcacggtg agagaggaga ccgacgatgt
    55981 gctggctggt attgctacaa tggcagccag ttcggatgtg atctttgatg cggtgagggg
    56041 aaagtttccc ctctttcttg agttctggga aaggtccatc cgcgatgagc gagtgtggga
    56101 agagactaaa gcgccgttca caaagtacca cctctatttc tcgcaaatga tcaaacacgg
    56161 cgttgcaaat ggcacgatca gacccgttga ggcggcaagc gcctcacgtg ccctgatcgg
    56221 tctggcgatc ggtatcattt tacaaagttt attgtatccg caggatgcgg attggaaagc
    56281 agaattgcag aacagtatgg atattctttt aagaggttta cagaatggat aaaggaaaag
    56341 ttttgatcac aggagctgcc gggcacttgg ggaatgtgct tgtgcgcgaa ttgctggagc
    56401 atggctacga agtgcgtgcc atggttatgc ccaatgaaga tgtgtcggca ttggtagggt
    56461 tggatgttga gatcaaattg ggtaacgtgc tcgaccctgc ctcgctggat cgcgtgttga
    56521 aaggcatcga ttatgtcttt catatggcgg ggatcgtatc gatcgtgccc ggcgaagaag
    56581 aattggtgtg gaatgtcaac gtggaaggca cacgcaatgt gctcaaagct gccagaaagg
    56641 cgaaggtgaa acgcctggtg tacaccagtt ctattcacgc tctcagccgg caatgggaag
    56701 gtaccatcga tgaacatgtt cccttcgatc cggataaccc tgtgggaact tatgatcgca
    56761 ccaaagctac cgcttctctg gcagtaaacc aggctatcaa agaagggttg gacgctgtca
    56821 tcgtctgccc tactggcgtg gtcggtccgt atgatttccg cggctcgagc atgggcgaat
    56881 tggtgaacga cctcacacgg catcacgttc acgtgctggt agagggagct tacgattttg
    56941 tggatgtgcg cgatgtggca gccggtcatc ggctggcgct cgaaaagggc aagaccggtc
    57001 aggtctatat cctttccggg caccaaatca aactgctcga actgaaagag atggtgcaga
    57061 agatcgtggg gttcaaaacc ctgacgatca acgttccttt caagctggcg aacattgggg
    57121 cagatatcgc cacgctgtat tataagatcg caaaaaagcg tccgaagttc actcgctatg
    57181 cgctggaaac cgtccagagc aactcggtga tcagcaatac caaagccagg cgagagttgg
    57241 gttattcttc gcgcgacgtg ttcatgacca ttgctgatac agtgaaatgg tggcaaacca
    57301 agaaaacccg caataacggt taaagagtga gatcgaaaac agcgctgcag ggtaagactg
    57361 ccttgatcac cggggcttcc agtggggtcg gcaggcaggt ggcgcttgac ctggctgccc
    57421 ggggggtgcg ggtcatgctg gtggcacgcc gtgaggatcg cttggtttcc atccaaaggg
    57481 agatcgaaaa ccacggtggt gaggcaattt accatgtgtg tgatctggag gatgaaaacg
    57541 cccgtcgggg gttgatctct catcttctaa aggacacgcc ggtggatatt ttgatcaaca
    57601 atgccggttt tggttggtac ggttattatg ccgatatgcc ctgggagatt gctcgtgatc
    57661 tggtgcaggt gaatattacc gccatgctgg cgttgaccag ggcgctgctg ccgggtatgc
    57721 gcgaccgtgg atacggtcat atcgtcaata tcggttctat cgtgggtggg tttcccaacc
    57781 agggagtggc ggtgtatgca gccagcaaag cttttctgga tgctttttcc actgcgctct
    57841 ttcgtgaaat gaaaggcagc ggggtgtggg tgagcgcgtt gcgccttgga ccggtaagaa
    57901 cggaattctt tgaacgagcc aaaaatttgc ccaacggtcg caaggtccct gctgaaactt
    57961 tttccatatc ggtagagaaa gccgcaaggg gcgtgcttcg gctgttgcag tcacccaaac
    58021 gctatctgta tgtgcctgcc gcgctcagtg ttaccgtgat catcggtctg ctttttgggg
    58081 gtttgattga ttctctcgga ccgctgctat tgcgaaagag cggggactga tgctgtgcga
    58141 tccggcgctc ttttcaggat cgcttgaaag gcaggggtat tcgctttcag ccgctcgctt
    58201 ctgcgaaaaa catcaacaag ctgcacccgg ttcccatggt cgagcatgat tttttccatt
    58261 acccatagcg atgaaacatt ggaaagtgat gggggtctga agagctgttg ctctccgcgt
    58321 tggcggagga cggtataggt gtttgcaccg aaagaacggc tgttggcgaa cgggtagcgg
    58381 gagaaattag ggtggaaata aaatctgctt tgcttgctcg acataccttt tgtaaaagag
    58441 ttcacttttc gcatgaatgg tggaggacgt tctgttcaaa aaacggtact tcctgcgttg
    58501 tgattcatgt ttaatctgct atgcttatta gaaagaaaaa gagtggaacg tttataaaat
    58561 gaggagaaaa aatgcagaca ctggataata ccatggcagt gatcatgggg ggtggacagg
    58621 gtacgcgact ctttcccctc acccaacagc gtgctaaacc tgccgtgccg tttgccggca
    58681 aataccgtct gatcgatgtc cccatcagca actgcatcaa ctccggcatc tacaagatcg
    58741 cagtgcttac ccaattcaat tcggtttccc ttcaccgcca tatttctaac acctataatt
    58801 ttgacgcttt ccatgggggt tatgtacaga tctgggcagc cgagcagacc ggcgagagca
    58861 ctgcctggta ccagggtacg gctgatgcgg tgcgcaagca gctttttgag atcaaaacca
    58921 ccggcgttcc tttcgttttg atcctcgccg gcgatcacct ctatcgcatg aattacgctg
    58981 acatggcaac ctttcattgg aaaaaaggtg ctgatgtgac cgttgccgtc caacccatct
    59041 cgcgcgaaga cgcaccgcgc tgtggggtgc tgaagcggga taaggaagat cgaatcgttc
    59101 gattcacgga aaaaccgcag gaggagaaat tgttaaccga aatggtcagc cagcctggtt
    59161 ctgacaaacc ttatttcggt tcaatgggca tctatttctt caaaaccgag gttctctgta
    59221 aactgctgga gcaaaccgat tacgacgatt ttggaaaaca tgccattccc gctgccgtgc
    59281 aggaatatca ggtctatgga tattcgtatg atggcttctg ggctgatatt gggaccatcc
    59341 gtgcttttta cgagaccaat ctggctttga ccgaagaaaa accaccgttc tcgttgattg
    59401 atgaagggtg gccgatctat acccatgcgc gtttcttgcc cggcagccag atcgagaaca
    59461 gccacattga tcataccctg ctggctgaag ggtgctggat aaaagatgcc agtatttcgc
    59521 attcggtcat cgggttgcgc agccagatcc gcgctggggt gacgatcaag gatagcatca
    59581 tcatgggcgc tgattattat gggttcttcc gttcggaggt gtcagagttg aaaagcatac
    59641 cgttgggcat cgggaacgat tgctacatcg agggggcaat catcgataag aatacccaca
    59701 ttggtaaagg gagtgccatt ttgccatttc cgcgcggcac cgagttggac actgcccact
    59761 ggatggtgcg cgatgggatt gtggtgattc ccaaaaacac tgtcctcagc ccgggcactc
    59821 gtattgcacc ctgacataat ctgtattaaa cttgagccat tgtcaaaatc ccaaacaggg
    59881 tgaaagatag gagaactgga atgactgcaa aaaatactgc agatgttcaa cgcgccgctg
    59941 atcgagatat cgagatcatc tggcattctc tcagtcaaga agaagtgctt aaaaaactgc
    60001 acagccctat cgaaaaaggg ctttcttcgc aagaagctgc tgcacgccaa gaaaaatatg
    60061 gtttgaacga acttcaggaa ggaaaaaaac gaaccttcct gaaaatggtt tttgaccagt
    60121 tgaacaactt cgtcatcatt atgctggtca ttgcagctat cgtttctgcg tttctcggag
    60181 aaattatcga cgcaagcgcc atcattgcga tcgtgattct gaacacggtc atgggtgtgg
    60241 tgcaagaaag ccgcgccgaa caggcgctgg atgccctgaa aaagatggct gctcctgaag
    60301 ctcaggtttt acgcgacgga caccgcatct cggtgccagc ccgtgatctt gtgccggggg
    60361 atatcgtttt tctggaagcg ggcaattttg tgcctgctga cctgcggttg ctggaagcgg
    60421 tcaacttgaa aattgatgag gcagccctca ccggagaatc agtgccggtg gagaagaatg
    60481 ctgcactgtt gttggattcc aatgcatcgc ttggtgatcg catcaatact gccttcagcg
    60541 gtacggtcgt ttcgtatggg cgcgggaaag ggatcgtcgt cagcaccggt atgaacaccc
    60601 aaatcgggtt gatcgctacc atgttgcaat cggtggaaga agaacagact cccttgcaga
    60661 tcaaattaga ccaactgggc aagttgctcg gttatgcctg ccttgtgatc agcgccatcg
    60721 tttttgtagc gggttggctg caaggcggcg accttctgga atcgttcatg gttgcggtca
    60781 gtctggctat tgccgcagtg cctgagggtt tgcccgcagt ggtcaccatc agcttggcgc
    60841 tgggcatgca cgagatggtg aaacgtcatg ctctcatccg ccgtctggct tccgtagaga
    60901 cgctgggttc tgccacggtt atttgctctg ataagaccgg cacgctcacc cagaacgtga
    60961 tgacggtcac cgaggtttgg acggatggta agaccgtgaa catcaccggt accggttatg
    61021 tgcccgaagg aaaatttgtt tttgatggca agaccattga gttaaatgaa gttcccgctg
    61081 ttgccaccac gttgtgggtg ggcagcgtca ataacgattc cgaactggaa gtcattgaag
    61141 atgagaacgg taagatgaat taccgcattg ttggcgatcc caccgaaggt tcgatcctgg
    61201 tggcggctgc caaggcgggt gtgcatgccg ctgatttaaa acgggcttac ccgcgcgagg
    61261 gtgagattcc ctttgattcc acccgcaagc gcatggtcac tgtgcatggc atcaaagaaa
    61321 cctggaaggc ttcgaatctg ccgttcaatg gaaagggaaa agaaagtgcc tatgtcattg
    61381 ctgtaaaggg cgcccctgat gtggtgctca agctctgcaa tcgctaccaa aaagctgata
    61441 acaccacggt ttccctcgat gatgctgccc gccggcgcat tttgcaggct aatgatggca
    61501 tgacccagaa agccctgcgc gtgctgggta tggcgtatcg tgtggtttcc gccaaaccgc
    61561 aagatgtgga cagcgagcac cttgaaaagg acctggtctt tgccggtctg gtcggcatga
    61621 ttgaccctgc ccgtgaagag gtgaaaccag ctctcatgaa agccattggg gctggcattc
    61681 gcactatcat gatcaccggc gattatccca acacagccaa agccattgcc gaaagcattg
    61741 gtttactgcg ccctgagcat cgcgtggtca ccggtgcgga tatcgaatct ctatccgatg
    61801 aacaattgca aaaagaggtc aaaaccatcg atgtgttcgc ccgtgtttcg cccgaacata
    61861 agatgcgcat cgtgaacgcc ctgcgcgcca acgatgaaac tgttgccatg accggggacg
    61921 gcgtcaacga cgcccccgcc atcaaaaccg ccgatattgg ggttgccatg ggcattaccg
    61981 gcacggatgt tgccaaacag acggccgata tggtgctgac cgacgataat tatgccagca
    62041 tcgtttcagc ggtggaacag ggacgcatca tctacagcaa catccgcaag ttcgtctatt
    62101 acctcatctc ctgcaacatt gccgagatca tgatcatttt cctgcccacc gtgatcggaa
    62161 aattcctctt cccctttgcg gctgcttcac tggctgtttc ttctccgctc aaacccatcc
    62221 agttgctgtg gttgaacctg gtgacggatg gcgctcccgc gctggcgctt ggcaccgaaa
    62281 agggtgatcc ggatatcatg gatcaatctc cgcgacccaa gaaagaaccc attatcaacc
    62341 gctttatgct ggtcggcatt gccatccaga ccattttcat cgccggtgcg acccttttgg
    62401 cttattcatt cgggttgcgc atgggcaacc agcaaaccgc cgagaccatg gcttttgtca
    62461 ccctctcgct ctccgaactg ctgcgcgcct acactgcccg ctctgaacgc tatccactct
    62521 tgaaaatggg cgttttcaaa aacaaatgga tgaacattgc cgtgctggct tccgttttgc
    62581 tggtcatggc ggtcatctac attccctttt tcaatgtcat tttcgatacc gttcccctga
    62641 ccccggtgga atgggaaaag atcatcccct tgctactgtt gccttcggtg gcggctgagg
    62701 tggtgaaata cgttgtttcc agaaaaaaga aaacagcata acgaaccgtg aaatatagaa
    62761 agggagaagc gttttttcag gcttctccct ttttagttcc cgtgcaccga acgcatgggt
    62821 tcaaagctga cgcgatggat ctcgcatagc ccggttgttt gcagtgcggt gcgatggcgg
    62881 gcagtgccgt accccttatg cgcggcaaat ccgtaggtgg ggtattggct gtctatttcg
    62941 atcatcaagg cgtcgcgggc tgttttagcc agaatcgaag ctgccgcgat gctcaagcac
    63001 aggctgtcac ctttgggcag gcagatttgc gggagattgc tttccgttaa tcgcatgtaa
    63061 tcgatcagca gatggtcagc cttataacgc agttgcgcca gcgcacgcaa catggcagcg
    63121 cgcgtggctg caacgatgcc catctggtct atctcctggt tgctgacaga acctattgcc
    63181 caatccaggc tggctgcttt gatacaaccc tgccagcgtt cgcgctgatg tggggtcatt
    63241 ttcttggagt cgttaacgcc gttcagcaat tgcagaatgc gctcatcgtg aggcagaatg
    63301 actgctgcgg cgaacaccgg tccagcccag gcgccgcgcc cggcttcatc cagcccggcg
    63361 atgctgtgat agccctgttg ccataactgc aattcgtgct cgagggtggg gcggtgagcg
    63421 gggggagtac gactacgagc catactcttt acggaaggct ttccagcgga tgcggatcag
    63481 atcggcagcc atgtgcaacg agtcgcgcaa aaatcttacc ttgctatgcg cattaaaatg
    63541 ccagtggatg ggcacttcgt ggatggcgta gccgaacctg cgcgccagaa agagcacctc
    63601 cacatcgaaa gtccaaccct ggatggtctg cagcgggaac aactgatcag cagctctggc
    63661 gctgaaacac ttgaagccgc actgcgtatc ctgcaacccg ggcagtgcca tcactttcac
    63721 caatccgttg aaggcacgcc caatgaagtg gcggtaggcg ggttctccga tgcgctgagc
    63781 gccgggcgct tcacgcgagc caatggcaat atcgcagccc ttcagcgcag gcgggatgaa
    63841 attgttgacc tcttcgatag gcatggagag gtcggcgtcg cagatgaaac gatattctcc
    63901 ctgcgctgct aacatacccg tcttgaccgc gatgcctttg cctcgttccg cgatgtggat
    63961 ggcacgaaag gtgggatatc gttgcgcgaa ttcccgggcg atggtgaagg tgtggtcgct
    64021 gctgccgttc tccaccacca gcacttccgc gcgatagctc tgctggctca aaaaagtagc
    64081 gatcgctgcc aggctggcgg gcaggcgcgc ttcttcgtta taggcgggaa tgacgatgga
    64141 caggaagggt ttattcatgc taagaactaa ttatacgagc attgagtaag ctatccaata
    64201 aaatagacta ggatttctgt atatcattga tttctaaatt atgtataaag tacaattgaa
    64261 atatataaag gtcagtatta atgatatttc ccccttgaaa ctgtttaata atcaaaaaaa
    64321 cgaataaaga ggaaatatga gtcctaaaga accacgagtg tgggaaaccg tttcgaaaaa
    64381 aaccatcctc aaacacagta aatttttaac agttgaaaat catataataa aacttcctgg
    64441 tgggcaggta atttccgatt ggccatggat tattattcca agtgctgcta ttgtccttgc
    64501 ggtgacggaa aacgatgaat tcctttgctt tcgccaaaca aaatatgcag tagaaggaac
    64561 aacgttagcc ccggttggcg gcatgcttga gctagatgaa aagccaatgg atgcagcgaa
    64621 aagagaacta cttgaagaga ctggctacat atcagcacat tgggtcagtt ttggaagtta
    64681 cattctggat cccaatcgag gtatagcaac catgaattta tttttggctc tgaaggcaaa
    64741 acaagtaacc gagcctaaca gtgatgatct ggaagaccag gaattgttgc ttctcagcca
    64801 aaatgaaatt gaagatgcat tgaaatcagg ggagttcaag atattagcat ggtcaacagt
    64861 tgtctcaatg tctttaaatt acataactaa ttccagaaac catggaatgc tttaggaagt
    64921 atctgctttt catatttcaa tacaacaagg cgaatacaat tattctgtga taattacagg
    64981 tcggggtggg cggacttgaa ccgccgacct ctgcgtccca aacgcagcgc gcttccagct
    65041 gcgctacacc ccggcgcatg aagtataatc ggttaacact tcaaccgtca aggagtgccg
    65101 tttgagcatg cgcagccagc cacgttctca agaaatcatc cggatgtttc ttttgctgac
    65161 ttctgcgtcc ctcttggtcg gctgtcagcc tgctgcgacg gtgccactgc aatcaacttt
    65221 gtctcccacc gccaccctgc ttcccagccc gacccccaac gtgcaattga cctcctcctc
    65281 tgcgcaaacc acggaagcac aaccgctgtg cgaggagagt ggcagcatcc gttcctacac
    65341 gctcgcttcc gagttgcttg gcgaagctct tgattttgac gtttatctcc ccccctgcta
    65401 tgattctgct tcggggcaga cttacccggt catttacctg ctccacgggc agtggcagaa
    65461 cactgctctc tggcaaacga ttggcattca gcctacagcc gatgaactca tcttgagcgg
    65521 gcaacgccaa ccgttcttga tcgtcatgcc gtatgaaaaa tattatttcc gtccggctga
    65581 gaataaccac tacccggatg cgctcgtgca agagttgctg ccgtgggtgg aagataatct
    65641 ggcggcatgc gggcaaaaag aatgtcgcgc catcggcggc atctcgcgcg gggctgcctg
    65701 ggcgacccgc ctggcacttc agaaccccga tatgtttagt gctctgggtg cgcacagcat
    65761 gtcccctttc aatggggata ttgaggagct gcccgactgg gtggctacca ttccgcaaga
    65821 tgaactgccg cgcatttatc tggatgtggg tgccagcgat ccgacggtca aagatgcatt
    65881 ggcttttgag caaactctca acaccctggg agtggcgcat gaatggcatt taaattccgg
    65941 tcggcataat gaaacctact ggtcggaaca gatcgcgaat tatctgagct ggtatacgct
    66001 gaattggatg gaggagtgaa gggaacagga tgatcctcga gaaaaaactt caatggctcg
    66061 ttctcatcac aatgttggga ggtattctgg ctgcctgttc tacccatggc gcggctgaca
    66121 ctgtgcccac cgcaacagca gcccttaccc ccactgagga gcttccaacc gccaccccct
    66181 ctccgcaagt gtgcaccgaa acacaaggat attttgagta tcaggaaatc cagaccgcgc
    66241 tcatgacgca cccgctttct ttccgcgtct atctgccggt ttgctacgac cccgacgggg
    66301 agtacgaata tcccgtgttg tacttcctgc acgggcagag cttcaatgat gatcagtggg
    66361 atcggctggg tgccgatgaa gccctcgata ggttgatcct ggcgcgagag gtagctcctt
    66421 tcattattgt catgcccaaa gaatccgatt atatgtcgaa ccagtgggat tcaaagtatg
    66481 gtccggcgat cgccgaagaa ttgaccgttt gggttgatga gcactaccgc acctgtgcgg
    66541 aacgcgaatg ccgtgcgatc ggggggcttt cgcgcggggc ggcttgggct atgcgcacag
    66601 gactgatcta ctggcagacc ttcggcagca tcggcaccca cagtctggcg cccttccgcg
    66661 gagattttaa cgaggcacct ttctggttca aagagatccc cgaagatcag ttgccccgta
    66721 tctggatcga tatcggcgtg ctggatgtaa acctggatgc cgccaatacc tttgaagtac
    66781 gtttgaccaa gtatcgtatt ccccatgaat ggcatatctt cctggggacc cataatgagg
    66841 actactggca ggcgcatgta gaggaatacc tgcgctggta tgccgaagcc tggaaggaga
    66901 agatggatca atgatcgcga atatcggata tttgttgctg gtgctggggg tggtgtgcag
    66961 tgtgctggct gtgctcatct gtttgtgggc attaataaaa aaagttcgtt cttttgtgat
    67021 ggaaaccagg gtgctgtctg ccatagggac gctggctgtg gttgctgctt tcgcattagc
    67081 tattgttttg ctggtaagcg gtcgctttga ttatgagatc gtttatgaga ataccagccg
    67141 ccagatggac ctgctccaaa aggtcactgc ggtgtggggc aacaaaagcg gctcgctcct
    67201 cttttggagt tttttgctct ccatctgtac ctcggttgtt gcttttggca aatggcagga
    67261 taaaaaagaa ccttattttc atggcgtcat gctgttgttg caactgatca atctcttctt
    67321 tgtagcgctt atcaccttcc aaaccaatcc ttttgcgcgc ttgtggcagt tgccaaacgg
    67381 gaatgtagcg gcagcagtgt tcgcaccagc gagtgctctc ccgttcaatc ccggtgacgg
    67441 gcagggtttg aacccgctct tgaagcatct cggcatggtc atccatccac cgcttctcta
    67501 tttgggattc atcctgttct ttgttccctt tgcttttgct cttgtttctc tatggcgtag
    67561 agattattcc ttcacctggc tgaacagttc ccgcacatgg atcgtgctgg cttgggtgtt
    67621 tctcactgcc ggcattctgc tgggttgttg gtgggcgtat gacattttgg gttggggtgg
    67681 gtactggagc tgggacccgg tggagatcgc cggtctgatg ccctggttga gcggcttggg
    67741 gttgctgcac tgtttcaaca cctaccgtgc cagcgcccat gtgcgacgtt gggcatatgt
    67801 atggatcgtt ctcaccgtgc tgcttatcat cttcggcatc tacctttccc gcacgggcgt
    67861 ggtcacttcg gtgcatgcct attcgcagag cagcatcggt ccggcattca tgggcttttt
    67921 catcttcttg tttgttctgt caattggcac attggtggcg cgctggaaag acctgcgccc
    67981 ggaggcagag cgcccatcct atctctccca ggcgggctta gcttgtactc tctctctctg
    68041 tatggcattg ctggtgctgg tctgcttagt gggggttacc cttccgctca cctcgcaagc
    68101 cctcaccggt agtcgcacgg agccctcgca attgtattat gagtggagct tcgctccgcc
    68161 cctgctggtt gttttgatca gcattgctct tgggtatctg ccgcagaaca agagttattc
    68221 cacgctttcc ggaatccttg cgcttgtgtt caccattggg gtaggcttgt cgcttggcaa
    68281 gatgaatttt tcggcgctgg tgggcttatg ggtggtgttc ttcttgataa gtgcgctgct
    68341 tttcgctgcc gctgatcgtc tccgccgttc atcttctagt gtaacgccca aaaaacgttt
    68401 cccgcgcctt gctggtatcg tgctgcattt gggatttgcc ctgctggcgt tgggggtggt
    68461 cggcagccgt aatttggcag attccaatgt ggtttctttg caagccgggc agagcaccgc
    68521 tttggatggc atgcaggtca caaagacttc tggagaggtg gaggttgcag cgaatcagca
    68581 ggtgctgtat gccgaagaat atgttttcca gcaggataac aggcgggctg tggtgctgca
    68641 tccgcacatc ctttggtacg acgacacccg cagcaccgtt tcttcccccg ccatccattc
    68701 cacgttgttg accgatacct atgctgccat cctggagtgg gatggatttg ccaatggcac
    68761 aaccacggtg ctgctttcta catttccttt gatggcatgg ttgtggatcg gtggcgtgct
    68821 gctcatgctg ggcactgtat ggttgcatgt tgcaaacagg ttttctcgaa aggacgacag
    68881 cgagtcggaa gtgcctgatc gaacagcaaa ataacatata aactacccac accttcttca
    68941 caaattcttc acatctcatc gatattctta cgtttgaaag atccatgagg tgtaatatgg
    69001 aaaaaggttg gaagatcgct ttgatcgtcg tcggggcttt tctcttgatc tgcgcgatgg
    69061 ttttcctcgt gtcgtatttc gcaggcggca ggttctatcg aggttatgac agctatggaa
    69121 gcgtacggcg ctgggatgat tctgctgatc gctacttcag aatgccgcac ggtggcatga
    69181 tgcgcagatg ggatggctcc tcagcaccca atgaagattt ttatcttgaa cattggatag
    69241 caattcttgg catcagcaaa gaggaactcc aaaaccgtct cgattccggt gagtcgttgg
    69301 aaagtattgc tgaatcgctg agcatcgaaa tgccttgttg ggataggaat gtggatgatg
    69361 agcatgctga cctgagcaca acccccacca gagatgatgg ttgggtcaat ggcgcaggga
    69421 tgtgtttcca ggaatacccc tgaaaaggaa cggaattgga gtattccaac cccgtttgct
    69481 tctgtataca atagcaatat gcagaaaatt ctcattatcg aagatgaatt ggaactggtc
    69541 aaagtgttgc gttcttattt ggagaatgcc ggttttactg tgatcagtgc tttgcggggt
    69601 gatagcgggc tggttgcctg gcagaacgaa cgtcctgacc tggtgctgct cgatctcaac
    69661 ctgcccggca tggatggcat cgagctcgcc aaatccattc gcaaaacgga tgccacgccc
    69721 attgtcatgg tcaccgcgcg agttgaagat atcgaccgta tcgtggggtt ggaactgggt
    69781 gcggatgact acatatccaa acccttctca ccgcgcgagg ttgtggcacg cgtgaaagcg
    69841 gtgctgcgcc gcacgcagcc gactgaagga tctgtgaaaa agttgatcat caaagacctg
    69901 gaaatcgacg cggatgcttt caaagtcaaa caagcgggca gcacgctcga tctcacccgc
    69961 accgaatttg ccattttgca gctcatggcg gaacatcctg gcaaggtctt tacccgcctg
    70021 cagttgctcg aagccactca aggggtggcg tatgagggat atgagcgcac cattgatgcg
    70081 catatcaaga atctgcgttc caaactgggc gataaagccg accagccgaa atatattaag
    70141 acctccttcg gaatcgggta tagcttcagg gagaattaat ctatgggcat gcgctggcgc
    70201 attttccttt catttctttt gatcatcctt gttgcggtgg gttcgttggc ttttttcgcc
    70261 tatcgttcca ctaccagcga aattgcctct tttgtaactc gcggcggatt atgggggatt
    70321 gaagaaatgg tgaaggcgct ggaagaatac taccaggagc atcaaacctg gcagggtgca
    70381 gaaacgatat tggcttcttc ccaaaccggc aaaggtatga tgcagcatag cggcatgatg
    70441 atgggtggta tgcacgagaa tgcctttcgt ttggtagatg cagatggcta tgtggtaatt
    70501 gatcagcgca atccgcaaag cgagggctct ttcctggata ccaatccacc gggagcggta
    70561 gtattgaccc tcgatgaaac ggtgattgga tacctgttgc ctgaaagcgg atatatgttc
    70621 ccgacggttg acatcaatgc agcgctgctc gaattgctgc tgccctcttc tatccgcgcg
    70681 gcagtcatta gcggattggt agggttgggg atggctgtca tcctgggata ttttctttat
    70741 aagcccatcc gcgccttgga aaaagcggct gccaacctgg caaagggtga tctcacccag
    70801 cgtgttggtg agacaggtcc ggctgaaaca gccaatctgg gtaagacttt caaccgtatg
    70861 gcgggcgagt tggaaaaagc cgaccggacc cggcgcaaca tgaccgccga tattgcccac
    70921 gagctgcgca cccctctagc cgtacagcag gctaatctgg aagccatgct tgatggggtt
    70981 tatccattgg ataagaagaa cctccaggtt gtgcttgatc aaaaccatac cttgcgcctg
    71041 ctggtagatg acctgcgctt gctttcgctg gcggatgccg gggaattggg tttgctgcaa
    71101 aagcccatcg aactgaccgg gtacctgcgc gcctttctgg aaaagttcaa gcctcgcctg
    71161 caagagaagc gactttctct gtacgtcgct ttgccagatt cggaaattgg aatgatcacc
    71221 gatgcactac ggctggaaca gatcctgacc aatctcatgc agaatgcggt cacccattct
    71281 ccccgaagag gaaaactgta ctttgcagtt gcacaatcag aagatgaggt gtccatcact
    71341 ctgcgcgata ccggcaaggg tatcaatcct gaactgctcc cggtgctctt tgaaaggttc
    71401 agcaaaggca gttcgaaaga ccgtgataga gaaagtaccg gtttgggatt ggctgtttcc
    71461 aagaagctcg cccaggcgtt gggtggtgcc ctgacggcgc acaattaccc cacaggtggg
    71521 gcggaattca ccctgacatt cttcataaaa aaatagaaga atcgcttaaa tctgcaaacc
    71581 tcgctataat tacgcgcatg agtctttcaa ggaaggaagt agaacacatc gcgttgctgg
    71641 cgcgcctgga tctcagcgaa gaggagatcc agcgttatgc ggagcagctt tcagccattc
    71701 tcgagcatgt tgctgctctg caggaactgg aaacggggca tattccaccc acctccagtg
    71761 tacttcccct gcgcagcggc ctgcgcgagg atgagccgca gccagggttg gacaccggca
    71821 gggcgctgca aaatgcaccg caggctgaaa aagaccagtt ccgcgttccg cctgttctgg
    71881 attaagttgt catggcggat ttcagaaaat actccattca agaattgatg acaggcttgc
    71941 gcgcaggcga tttttccagc cgcgaagtga cctcctattt ccttgaccgg atcgaaactt
    72001 tggaagggca ggtgcatgct ttcctttccc tcaaacctga acaggcgctg cagcaagccg
    72061 agcgtgcgga tgcggtttac gttgcgtggc gggctgaagg aagacagtct tcacttccag
    72121 ctttgctggg attgcccatt gcggttaagg atgtgctgtg tgtggatggt ttcccctgca
    72181 cctgtgggtc gcgcattctg caggattttc atccgccttt caatgccact tccgtgcaac
    72241 atttgctgga tgcgggggtg gtgatcgtgg gtaaaaccaa tatggatgaa tttgccatgg
    72301 gttcttccac tgagaattcg gcgtttggtc ctacccgcaa cccctggaat ttggcacatg
    72361 tgcccggcgg ttccagcggt ggcagcgcgg cagcggtagc ggcgcatatg atccctgctg
    72421 ctctggcaac cgacacgggc ggcagtgtgc gccagccggc ttccttctgc ggtgtgaccg
    72481 gtgtcaagcc gacctatggg cgtgtttctc gctttgggct cattgcctat ggttcctcac
    72541 tcgatacggc gggtgtgatg gcgcacaacg cacaggatgt ggaagcattg ctgcgcatca
    72601 tggcgggttt tgacccgcgc gatgctacca ccagcgacct gccggttttt gaccccgctc
    72661 ctcgcgaaag cgatctgaaa ggattgcgca ttgggcttcc actggaatac ttcattgaag
    72721 ggatccagcc tgaggtcgaa atggctgtga aagaagccgt gcgcttgcta gaagagcacg
    72781 gtgcccaagt tgtgcaggtt tctctgccac atacccggta tgccctgcca gtctattacc
    72841 ttattgctcc tgccgaagca tctgccaacc tggcacgttt cgatggcgta cgctatggat
    72901 tgcgcgtgga agatgaagac ctgatcccca tgtattcaaa aacgcgcggc aagggttttg
    72961 gagccgaggt gaaacgacgc atcatgcttg gcacctatgc cctttcggct ggctattatg
    73021 atgcctacta cgggcaagca cagaaagtgc gcaccctgat caagcaggat tttgaaaaag
    73081 ccttcgaaat cgtggatgtc attgctgcgc ccatcgctcc tactacagct ttcaaacttg
    73141 gtcagcacac cgatgatccc ctggctatgt atctggaaga tgtgtttacc ttgccagcca
    73201 atctggcggg ggtaccgggg ttggcgtttc cggttggttt tgatgggaat agcctcccta
    73261 ttggcatgca gctcatgggt ccgcaattct ccgagcaggt tcttttcaag actgccaatg
    73321 tataccaatc tttcaccgat tttcatcgtc gtatcccgtt aagcgtgcag taactttggt
    73381 ttatgagtta ttacggaggg gctgatgaag ttgcattgcc aaccgatcgc ctagccgatg
    73441 ggtcatcatt cacctgcaac ctgactgtca ggaacgacgt ggtcacgcag ctttcgttgt
    73501 tattaaacaa caaactctga tattatcggg atcgttgtag gtcgcaccgg cattgaggga
    73561 tctctggttg aaataaagaa aaccccgctt cggcgcggtg acataagaaa catataaaac
    73621 ggtgagatca atatcctcac cgttttatgt aatatactta ttttatcgga ttgttttact
    73681 ttttccgaca tatatagcgt gcatacacat ttcaattatc gcgggttcgt cggccgtttc
    73741 atttatcaag tttatcaccg ccacatattt ttcaggattt tcatccttta atttatatat
    73801 ttgttcttct cgactacttc cccatcctcg gatagagcgt agcaaaacct ttgaaaaccc
    73861 atttttttcg aataagtctg ctacttcatg tgatgtaggg taataacctt cttgaaaacc
    73921 tttgttggca ttattgttga agataccatt atcaaatacc cgttttagcg tttctgtact
    73981 tacttggtca ggaaaataga acattcttcc tgctactccg attgcgcctg tgagataggg
    74041 aataaagctg gcgaaaacca atccgttcgg ttttaaaacc ctgtttattt ctcttacgca
    74101 ggaaacacgt tcttcttcgt tagtcagatg atagagtggc ccgaaaagca aaaccgcatc
    74161 aaaagaacaa tcgttatatc tgcttaaatc tactgcattg acttgggtaa tggatttcac
    74221 attgctaatt ccctgctcgc taattttctt ttttgcttcg tcaagcaaag tctgcgataa
    74281 atcagctaaa tgcagaatat gacctgattt tgctaattca atggtatatc taccagggcc
    74341 gccccccaaa tctaatatct cagcttttgg gggcaagtgg cttgtgataa taggcataga
    74401 caaatcgaac tctagcctcc cttctggtgc gtcaagtcta ccccattcat taaatacatg
    74461 ataatatgct tttatttttt caaaatcggt cataattcca tcccgcaata tagaaatatt
    74521 atccatattt ttattatagg atgtatccat ccttatttgc tgctacattt tcgaaaaaga
    74581 aagccgatgc cgctatcaaa ataacaaaat cggcttttat ttccttatgt cagcgcgtta
    74641 tttgccggga tcttttcttt aagccgtata ataaaaagcg ttcatattaa gaaaagagga
    74701 atctcacatg caattcaaaa accccaatac tcagacctta ttaacctggc agtggcagaa
    74761 ttttgaacca tacttcactg atctgctagc ccttgaactg aatggttcca atattaccgc
    74821 ctggctgacc gattggacgg atgtcgcatc agcggctgat gagttgttca accgcctata
    74881 tgtggctact tcggtgaaca ctgtggatga agcagccgag cagcgtttga aagatttcat
    74941 ggaaaaaacc taccctccca tcatggaaaa aagccagcag ttgaaagaaa agttgctggc
    75001 aagtaacctt accccgcccg gttttgagat ccccctgcgc aacatgcgct ccgaagcggc
    75061 tctctttcgc aaagagaaca tcccgctgga agtggaattg aaccagctca gtgtggagct
    75121 cgataagatc cttggtgcgc agacggtgga atgggaggga gaagaacgga cagcccgcca
    75181 gatggaagtg gtgctgcgcg ataaagatcg tgccaaacgc gctgccgggt gggagctttc
    75241 gttcaagcgc caactgacag atcgccaggc gatcaacgaa aactgggtga aaatgttcga
    75301 gatccgccag caggtggcaa agaatgcgga taaacccagc tatcgcgaat atatctggga
    75361 gtcctataaa cgttttgatt acacccctgc cgattgtatg cgctttcatg atgcgattga
    75421 agaggtggtc gtgccggctg tcaatcgcca atcggaaaaa agaaagaaag ccctgggtct
    75481 tgctacgttg cgctactacg acctgttcgt agcgttggat gattacccac ccctgcaccc
    75541 tttcagcgat gctgccgaac tggaagaaac gacttccacc atcttccata aggtgcattc
    75601 ccaattcggt gaatatttcg agatcatgcg ccgtgaagga ctgctggacc tggcaaatcg
    75661 caagaacaaa gccgatggcg catacaccac cgattatgcc atgatcaaac gcccctttat
    75721 ttttgggaat gcggtgggta ttcatgatga tgtgcagacc cttgtccatg aaggcgggca
    75781 tgccttccat gcctttgaaa cctacaagtt gccctgtatc cagcagcgct ggacccccat
    75841 ggagttcaac gaagtggctt ccatggcgat ggaaaacctg atcatgccct acctcacaaa
    75901 ggaccagggt ggcttttaca gcgaacggga tgctgcgcgt gctattatct cgcacctgga
    75961 agataatctg cgtttctggc cgtacatggc gatcgtggat gctttccaac attgggcata
    76021 tgagaatccc aaaggcggat tggatcctga cgcgtgtgat gccaaatggt cagagcttga
    76081 agatcgtttt cgcccacact tagattggag cggtttcgaa gatatcaaaa tgaccggctg
    76141 gcatcgtaag caccatattc accaggcgcc gttctattac atcgaatacg gtctggctca
    76201 actcggcgct tctcaggtgt ggttgaacgc gctaaaagat caaaaatacg cggtggagaa
    76261 ataccacagc gcgttgaaat tgggtggcac cgccacactc ccgcagttgt atgaagccgc
    76321 gagcgtgaaa ttggctttcg atgccgcgac tttgaaaaaa gttattgacg tgatggagga
    76381 taagatcacg gaaatggaag ccaaactgta aaatacgtca gaacgggcgc aagcccgttc
    76441 tctttatatc atcgagcagg gcagctttcc atagcaagca gcggcagttc ccaccgcctt
    76501 gatcgatatg ctatcttgag cacagcgagg gatctccaaa aattaaaaga accatccaac
    76561 aatgaagaaa ccaatgatcg atttcaagta tctacacaat tcattgcgat ctgtcgagga
    76621 atcctctaaa aaatggagag attcctccat cactgcgttc cgtcggaatg acgttggaga
    76681 aaagaaagtt agcagggcgg caatggaatg ccgcctatgt aataattaat ttctccctca
    76741 cctcatttac aatgagggag gttagggggt aatattctta aattattctc cttttttcat
    76801 ctttcatgat ttaatcagag ccaattagtt cgggaggaat acatgcagca attaatagac
    76861 ctgatcaaaa aggatgggat ttacctggga aatggcatcc ttaaagtgga ttcctttatt
    76921 aatcaccagg tggatccaag aatcatggag gaatgtgggc gcgagttcgc gcggttgttt
    76981 gcctctaccc atccaactag gatcctcacc gcggagatct ctggtatcgc cccagcgctc
    77041 atgaccgcca agtttctcga tgtgccggtg gtgtacgccc gcaagcacaa acccattacc
    77101 atgcccgata cggtctacct cacggttgca ccgtcgcaca ccaagggcgt gatggttgac
    77161 ctgatcatct ccccggaata tctgcagccc ggcgaacggg tgttgatcat cgatgatttt
    77221 ctggctagcg gtgctaccat caatgggctg gtgcggctga cccaggctgc tggcgctacc
    77281 ctggttggca ttggagcgct cattgagaaa gagtttgagg gagggcgcga aaaattgtca
    77341 gaactgggtg tgcctatcga atcgctggca tgcatcaccg ccatggatga gaatgggatc
    77401 gccttgaaag aatgagataa taacaggcag agaattggaa tctctgcctg ttcgtttaaa
    77461 aaggatatgc catgtcgatt gccatccttg actacggatc acaatatacc cagttgatcg
    77521 cccgccgtgt gcgggaactg caggtgtact gcgaattgtt cccctgggat acacccgcca
    77581 gtgaggtgct tgccatccag ccgcaggggg tcatcctttc gggcggaccc gcttcggtgt
    77641 atgaccccgg agcgccgacc ctgcctgaat acgtgttgac tggcgaactg cctgtgctgg
    77701 ggatatgtta tggcatgcag ttgctggcat acatcctggg cggcaaggtc agcgcttctt
    77761 cccagcacga atatggttcg gctgaaatca gtttgataca aaagaacagg ttgatccctg
    77821 aagagattca cagggtgtgg atgtcgcacg gagatcgtgt ggatggaatg ccgagcggat
    77881 ttgccctgct tgccaccagc gaaacctgtc cggtggcagc catgggagat gaaacgcgac
    77941 gcgtgtatgg catccaattt catcctgaag tggcacacac ctctcagggg attcgcattt
    78001 tgagcaattt tctctttgat atctgtgatt gccgaccgaa ctggaccgcc caatccatca
    78061 ttgctgacgt ggttgagcgc gtacaaaaac aagttggcag ccagcgcgtg ctttcagcag
    78121 tgagcggcgg ggtggattcg agcgtggcaa ctgcattggt gcaccaggcg gtgggagccc
    78181 aacttgaagc ggtttttgtg gatacggggc tgctgcgtca gggagaacgc gaaagcgttc
    78241 aggcagcgct tacccccttg cttggtgagc acctgcatgt ggtagacgcg tcaaccacct
    78301 ttctttctgc gctacagaat gttaatgacc cggagcaaaa gcgcaaaatc attggcacaa
    78361 ccttcatccg tgtttttgaa gagcaagccc gccggctggg cgatctgcct tttctggtgc
    78421 agggcaccat ttatccagac gtggtcgaat cgcgcgcacc agaacgttcc aaagcgcagc
    78481 gcataaaatc gcaccacaat gtgggtggat tgccagaaca attgagcttc aaactggtgg
    78541 aaccactgcg ttatcttttc aaagatgaag tgcgcgcggt cggtgaagca ttgggtttac
    78601 ccccggaact gatctggcgc cagccctttc cgggacctgg attggcggtg cgttgcgtgg
    78661 gagccatcac cgccgaacgc cttgccagtt tgcgtgcagc agatgccatt ttccaaaatg
    78721 cattgaagca ggcagggctg atcaaccagc gtttgaaaga tggagaaact cccattgccc
    78781 aggcgtttgc tgcgctttta ccggtgcgtt cggtgggtgt gatgggcgat caacgcacgt
    78841 atcaggaaac ggtggtgctg cgcgcggtga ccagccagga tttcatgacc gctgattggg
    78901 cgcgcctgcc ccaggatttg ctggcgtctg tttccaaccg cattgtaaat gaggtgcccg
    78961 gcgtgaaccg cgtggtatac gatattacct ccaaaccccc ggctaccatt gaatgggaat
    79021 aaaatcattt tacaggattt cgagtttcgc gatagctgcc gaaagtttct ttaaccccgc
    79081 tttatcaaag gctaccgtca cgacctcatc atctccctgt aatttactct gaatcacaat
    79141 cccgtttccc aaaacggcat gcttcacgtg catgccggcg ctatattgcg cgctttcttt
    79201 gggtgcgctt ttgggttttt cggatgtgga ggtccaacgc ggttgcacgg attgcaggga
    79261 gaaggatttt gaacggtaat gttgaagccc ggcccgcagt aacaggtttt ctggaatttc
    79321 atccaggaaa cgggaaggaa cgctgtaatc aaaactgccg aaactgcgcc gttggtcagc
    79381 gcgcaccaaa tagagatggt ctttggtgcg cgtgatgccc acgtagaaca aacgccgttc
    79441 ctccgccatc tcttccggat cgtccatcga acggcggtgc gggagggtac cttcatccag
    79501 tccaatgatg aacaccactt taaattccag ccctttggct gcgtgcaggg tcagcaaagt
    79561 aggagaatcg ctcttctctg gtagggtatc ctggtctgaa accagagcca ggttttccag
    79621 aaattcaacc aaccccactt gagagtattc atatgccatg cgcagcagct cttgcacgtt
    79681 ttcccagcgg ctgtaaccca cttcgctgct ggcatctgtg atgaattcct gatagttggt
    79741 gtccttcagg atatgctcaa agaggtcagg cagcaggatg gtcgggctga ggacacgcca
    79801 gtttgccagc agagcgccaa aagctgccaa cgcgctggca gcccgcggac tgaaggcatt
    79861 ccaaaaaggc gaagcgtctt gtttggaacc caggtccagc agcacgctgc ccatgctcaa
    79921 ctccgccttt tcagcgcagg tgcgcaaatc cttgatggtt ttatccccga ttccgcgtgg
    79981 cggcaggttg atgacccgca acaagctcac ctcatctgca gggttttgca ccgtgcgcag
    80041 gtatgcgatc atgtctttca cttcttgccg tccgtagaaa cgctgtgccc ccaccagccg
    80101 gtaaggcatg cggctctgga agaacgcttc ttccagcaga cgtgattgtg cgttggtgcg
    80161 gtacatgatg gcgatatctc cacctttggc tttcttgctt tccaccaagg cacggatggt
    80221 ttccaccgcg aacatagctt ctgcatgatc gtctatggct tcatataaca cgatcttgtc
    80281 acccttccca cgctttgaga acaatttctt gggagtacgc tggctgttgc gatcgatcac
    80341 cgcgctggct gcatccagca cattttgggt ggaacggtaa ttttgttcca gcagcaactt
    80401 ggtgtggttg ggataatctt tttgaaagcg caacacgttg cgataatcgg ctccgcgcca
    80461 gcggtagatg gattggtctt catcgcccac cacgaaaata ttttgataat gagaagccaa
    80521 cagttgcagc agttcatatt gagccatgtt ggtatcctgg aattcatcca ccagcacatg
    80581 gtagaaacgg cgggcatatt tctctcgcac ggcatcgttt tcttgcaaca ggcgtacggc
    80641 atatagcagc aggtcatcga aatcgagcgc gttgctggct tgcagttttt gttcgtactg
    80701 ttcaaagacg cgacggatta cttcttcacg gtaactgctg gctgcaaaat ctttgggctg
    80761 gatcaggttg tttttcgcat tggaaatagc agcgtgtaga ctggcaggag ggtacaattt
    80821 gtcgtcaata ttgagttcct tcaagctcag cttcaccaag ttctgctggt cgtcggcatc
    80881 cataatgaca aaatcgcgcg tgaacggcag cgcgtcagct tcgcggcgca ggatgcgcgc
    80941 acaaatggaa tgaaaggtgc ccaaccataa cccatccgct ttgccgccca ggctgcgctg
    81001 cagccgttct tgcatttcgc gagcggcttt gttggtgaaa gtgactgcca aaatacggaa
    81061 agggtcaacc tcgcgttcca ggataaggaa cgctgcgcgc tgggtgagca cgcgtgtttt
    81121 gcctgagcca ggtccagcca gcaccagggt ttgtccgtct ggtgcgctca ccgcagcttt
    81181 ctgttggggg ttgagatcgg aaaaaatatc gttcatgatt gaaagatgat tttaatggat
    81241 aaacggtgtg aaaacagaac ccaaccttaa aaaagaaaaa gcgccggggg ctccggcgct
    81301 ttatggctat ttttgagcgt agagttcaat gatcctgacc actacgtcta ccgctttttg
    81361 catggattcc agtacgatat attcataggg tccgtggaaa ttatgcccgc cggcgaacag
    81421 gttgggggtg ggcaatccca tataggaaag ctgggcgcca tccgtccccc cgcgaatggg
    81481 ttgttctatg ggggtgatac cgctatcgcg catagcctgc ttggcggtat ctacaatatg
    81541 catcaccggt tcaagcacct cgcgcatatt gcggtattgt tccgtgattt cgacttttac
    81601 tgtgcctgcg ccgtattttt tgttcaagaa ttcggctgtg tccaggatgc gctgtttctt
    81661 caattcaaac ttgacagcgt cgtggtcgcg cagcaggtaa actgcctgtg tttgctccac
    81721 tgaggctgcc ggcatcttcc agaggtgaaa gaacccctcg cgtttttcgg tgtgttcagg
    81781 gcgctgatct tcaggcagca ggctgtcgaa ttcaaagaat acacgctgcg agttgatcat
    81841 gacatctttg gcatctcccg gatgcaccga ttttccggtc atggtgatat aagcgcgcgc
    81901 ggcgttgaag ttctcgtatt ccagttcgcc cagcggaccg ccatccaggg tgtaggcaaa
    81961 atcggcgcca aactgctcca catcgaactg ccggatcccc agcccgatct cttcatcagg
    82021 ggtaaaagcg attttgacgg tgccatgttt gatttcaggg tgtgcgagca gatactccat
    82081 ggcgctcatg atctcggcga taccggcttt atcatctgct cccagcagac tgctgccgtc
    82141 ggtagtgata atggtttgcc cgatatattt ctttagatca ggaaattctt ttgaagaaag
    82201 ggtacgggtg ctgcccaacg ggatgtcctt tccatcatag ttttcaatga tttgcgggtt
    82261 gacgttctcg ccgttgaaat cggagctggt gtccatgtgt gccaggaatc cgatcaccgg
    82321 agtctttttc tcggtattgg caggcagggt agcggtgacg aaacatttct cattgagctg
    82381 tacatcctgc attcccagtt ctttgagttc ttcttcgagc acgcgcgcca ggtcccactg
    82441 cacttcggta gaaggggtgc ggtcggtgat ctcgtcattc gagcgggtgt tgacgcgcac
    82501 atatcgtaag aatcgttcta ttaatgtcat gagctgctcc ttggttgtac aaatgattgc
    82561 ttgggtatct tatcttctcc agaagaaaaa atcaagcgcg aggactcctt tttcgtgcag
    82621 gcatttgcca ttcatctttg tttacgctac aatcacggca tggcaaattg ggagatgatc
    82681 gggcaggaat gggcggtgag catgttgcag cagcatgtcc gcagtgagca ggtacgccat
    82741 gcctatttgc tgtgcggacc gcaaggggtg gggcgccgca cactggggct gcgttttaca
    82801 caggcgctga actgtcccaa ccccaaggaa cccggcattc cctgcgggat ctgccgtatg
    82861 tgcaatcaga tcgaagctat gcagcaggct gacctgcatg tggtgcaggc ggagatccag
    82921 ggcggcatcc tcaaagtgga aaatatccgt gacctgctgc acgatctttc ccttaccccc
    82981 tatgagagca aataccgcgt ggcactcctg ctgcgttttg aagaagccaa tgaaagcacg
    83041 caaaatgcat tgttgaaaac gttggaagag cccaatccgc gggtggtcat ccttgccacc
    83101 gctgagaata cggagaaatt attgcccacc atcgtttcac gctgcgaggt gttgcgtttg
    83161 cgaccggttg cgccagatgt gctggctgca actttgcggg agcgcgatgt ggaagcacaa
    83221 gatgctgacc tgctggcaca tgtttccggg gggcgcccgg gtgctgcctt acggatgcgc
    83281 acggaacctt cgttattgga aaaacgccgc caatatctgc aagatctgct cgatctggaa
    83341 caaagccctc tacgtgaccg ttttaagtat gcagaccgca tcacccatta taaagatggt
    83401 ggacgggagg agaccaaaga agagctgcgt gcacagctcg agacctggct ttccttctgg
    83461 cgcgatgtgc ttctgaccgc cacgaatgtg caagtgccgc ctggcaaccc tgactatgcc
    83521 gagcaagccc gcgctctggc ttccaggctg ggggtgcgga gggcaaaagc caaagtgctt
    83581 gcccatcaac aggcgctgga gcggctctac tttgccaatt tgcgcctgct gatggagatt
    83641 gtccttttgt agcggctggg caaattgtgg gggtgagata ctgatctgat aaaactggca
    83701 aaaaatcatt tgtcccgcct agctgaaatt tctcagctct attcaaataa aaattgaaaa
    83761 taaaatgaga cttacttgtt gacctttgaa accaaataat tgtatactgt atacaaacaa
    83821 ggatgatgac gaataatctg ccaactataa ggcgaaaaaa ttatgctcag caagcccgcg
    83881 acattattga aacggcaatc atgcgcggag atttcgcatt gggcgaaaaa ctggcagaag
    83941 aaaatattgc cagacagtta aatatcagca gagttcccgt tcgcgaagca ctgcgcgcct
    84001 tggagaagta tgggctggta gaggtcaaac ccggtaatgg ggtgtgggtc atttcgcctg
    84061 atgcgaagga tattgaagaa gtatttgata tcagggcttt gaatgaaccg tatgctttag
    84121 aactctgctt taaaaactcg cctgagaaaa cgattctcga acttgagcgt caaatagcgg
    84181 attttgagag ccttcttgag caacatggtg gtcgatatga tttgatcaat caagaccttg
    84241 agtttgatga gatcatcttt cttcaatgtg gaaataataa attgattgag ctatggtcaa
    84301 tcttggcgcc gattatcaaa attggatttt tccacaatcc ctacttcaaa gaaaaaaata
    84361 gtgttgtgaa caatcgatct catcgggaaa tcgtgacagc tttcaagaaa ggacagattg
    84421 atacagcgaa aaatttattg atcgatcata tcttaaaatc caagaaatta atttgcgagt
    84481 cttttaagaa agaagaggag tagccatcat ttaattttct ctcaattgtt tgtggcgtat
    84541 caatccacat gacgcataca actttttcga ttgaggcgat agaaatttcg gcgaaccgta
    84601 acttgctgac gactgatccg aaggagcaat tatgtgaaaa taattaccgt tacatttgag
    84661 caataactta aataagaaag gtaaaaggac atgaaaaaaa caatcactca aatttatgga
    84721 atcagcagcg tacatgatgc aaaaatggta gtcgatatgg gcgccgatca cctgggtgtg
    84781 gcgtatggtg aggttggcaa aacccctggc tttttagatt ttgaccaggc tcgtgagatt
    84841 ttcgaatgca tcggggataa agcagtgaag gtaggattaa ccgttgcttt cgatcttgaa
    84901 gagatcgtag aaatggtcaa tacgctcatg cccgatattt tgcatctctc tggcgatatt
    84961 gccaagtttt cccctgagca agtcaaaaaa ttgaaggagc gtgtaccggg attgaaggtc
    85021 atgcaggcaa ttccagcgaa tgatcctaaa gtctatgaat atatcaatgc gtatgaggcg
    85081 gtctctgact attttctgat tgatacggtt gccgagggaa tcgtcggaat tggtgccagt
    85141 ggacttaccc atagcctcga aattgacaaa aagatcgtcg aatccacaaa ggttccctgt
    85201 atcattgcgg gtgggttggg accggaaaat gtggcggaag ccattcgtgt tgtcggaccc
    85261 tatggggtgg attctctgac gaaaacgaat tgggataaat cgaaaggaat tcgtggaaaa
    85321 gaccctgaga aagtgaaagc gttcattgaa gctgttcgag aggtagaaag ggaatgacag
    85381 agggagtatg cgtattaggc gatacagcgg ttgatctcat tgtccgtttg cccgagatca
    85441 acaatggtgt aaaggtcatg gcgcatgccc ccaatttgaa tccggggggt agtggcgcga
    85501 attctgcagt tgccctcgcg cgtcttggaa tacccaccca gttcattggc acaattggag
    85561 aagaccagta cgggcaggtg gttgaacaag atttcataaa cgagaaagta ggggttgatc
    85621 aactgtttgt tgaaccaaca ctcaatacgg tttgtgtatt cgctttcata gaccaagatg
    85681 gagaaagatt tctttgggga tggccaaaag agaacgcctc gtttatttcc attgaattcg
    85741 agagaataaa tcttgatcgg atcaaagaaa ccggttggct gcataccacc ggaatcctgt
    85801 tgggaactga atcgagtggt cgtgatgcga ctcttaaagc tctggaatgg gcacacaaaa
    85861 atggagtgac cacgtctctt gatttaaacc tgcgggttgg cgaaagtgga caagcgcttg
    85921 aaaaagaatt ctatgatgtt gtaatgaatg ctgtatccaa ttcgaagtat gttttgggtt
    85981 ctgatcaaga gttctctctg ttggatccct caaataactg ggaaaaaaca gtagaaaaat
    86041 tggtgaaatt ggaaaaggtc gcagttgttc ggcgcggaaa agagggttct gttttgatca
    86101 ctgacggtca acgaataagc tcgcctgctt ttgaggttga tgtggttgac acaattggtg
    86161 ctggcgacgt tttcgatgca ggcttcattg cggcaattct caagaatcat gatttttcaa
    86221 ccgcgctgca atggggaaat gcggttgcag gatacactat ttctatggaa ggcgccagaa
    86281 ccagcccgac atttgaacaa atggaagact ttattcgtaa aaagatgggt tgatgaaatg
    86341 gagaatcacg tgaagaaata tattatggat tgtgatcccg gacatgatga tgctgtggct
    86401 ttaattttag c
//
LOCUS       DAOSDS010000002        79977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_225_length_79977_cov_5.814369, whole genome shotgun sequence.
ACCESSION   DAOSDS010000002 DAOSDS010000000
VERSION     DAOSDS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 79977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 79977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79977
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_225_length_79977_cov_5.814369"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PLL88_00410"
     CDS             <1..748
                     /locus_tag="PLL88_00410"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOJ00050.1"
                     /translation="GAGIDGSLSGNEGFPEWLRQSANNFWGWPIPNAEKTYPVQDDAD
                     LIILVVRQSQEPVTLFFSGPFTNLALALRKAPEIRNNIAALYMMGGAVYVPGNVHDFY
                     PDSANVYADWNPYSDPQATKEVFESGLQMYLIPLDATNQVRIDKSDTFRWREGGEIAN
                     FAADIYDGLMDSTGKEEFYIWDLMASEVMLNHALCDFTPLHLDVITEESDHFGQTVVV
                     PNEEPNINVCLKPKVDLIKQGLIDAFSNSR"
     gene            1380..2261
                     /locus_tag="PLL88_00415"
     CDS             1380..2261
                     /locus_tag="PLL88_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HOJ00051.1"
                     /translation="MPFNQNSRLDPSQVQDRRGRRVGRTVAIGGGGVGLIIVVVALLL
                     GADPGDLLGMVDSNLQLSDQNISEISDLQAECQTGADANTREDCRIVGYVNSIQEYWS
                     SEFANYGVQYTQAQTVLFSGLTEGACGYASAATGPFYCPDDQMVYVDLSFFDDLTTRF
                     GAQGGPFAEAYVLAHEYGHHIQNLLGALDRNSASSTGPRSMSVFTELQADCLAGVWAY
                     HAAETGYLTVPTSEEIAQSLDAASAVGDDRIQRETQGYVSPESWTHGSSEQRQAAFVD
                     GLQSGDINTCNTPGWTE"
     gene            2462..2749
                     /locus_tag="PLL88_00420"
     CDS             2462..2749
                     /locus_tag="PLL88_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00052.1"
                     /translation="MSSRGQCYNFAMNEIYSDTHPKIAALQRKMLRQASPARKLAMLG
                     EMNKTVKVLALSGLRSRYPDETPEMHKRRLADLILGPSLAGLVYGPLIDNR"
     gene            2910..3431
                     /locus_tag="PLL88_00425"
     CDS             2910..3431
                     /locus_tag="PLL88_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00053.1"
                     /translation="MSSIQTDMDSFQSQLVKGSIQRAYRALLAYMLALRSQFASKYGE
                     PAVSALYQGYMDMTYFALFPPSLKCLDLKIAIVFNYDAFRFEAWLAARNRKVQRQYWE
                     LFKNNDWAEYRVVAPAAGIDSILECDLAKDFDLSKPDALTSSIEMTTVAFIDKIERFL
                     SEQQPHEGATSCS"
     gene            3938..5506
                     /locus_tag="PLL88_00430"
     CDS             3938..5506
                     /locus_tag="PLL88_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ00054.1"
                     /translation="MKIKKTSPNPVLLIILLIFLLFGCTTAIRSETAAVEEKTPTPIT
                     SNQKLRIRLLTTSDWTIFNLISGATWVTKELISTSPEATTAKIEQNQFTLDQPIDSAE
                     AGKGVEMTVELLLSPLATENELVFEIERGDIGYTQVEFFGFSGTNWEVMKTVQWAGKS
                     GDGVNLYSVEIPFAELIEIAPTTIVDSQQEDTSKIDNSKNIQGTEEYPWWNDSVFYEI
                     FVRSFYDSDGDGIGDFNGIVEKMDYLNDGNPNISTDLGITGIWLMPINPSPSYHGYSV
                     TDYYSVNPEYGTMDDFKNFLNEAHKRGIKVIIDLVLNHTSNQHPWFLNAADPTSPYHD
                     WYIWSDTDPGYVGSWGQKIWFEQGDEYYYSTFSEFMPDLNYTNPEVTQEMQNIVQFWV
                     NEVGVDGFRLDAAKHLIEEGIQQANTISTHSWYEKFRPALKQINPHALTVGEIWEDTR
                     INAEYLQGDEGDPVTDYRLSLEASNLPGGNYVPAPILGGGDFMPLEINSNGGFSRYTP
                     ILEVPPYAIITLGF"
     gene            complement(5628..6398)
                     /locus_tag="PLL88_00435"
     CDS             complement(5628..6398)
                     /locus_tag="PLL88_00435"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ00055.1"
                     /translation="MSDFEFSKLQDSIENVPQRAYEYLKLLINNNSLKIGTRLPSEQE
                     LTEKLNISRSSLRIALSRLEVEGFIKRRHGVGTFITGNAKTLMPVRIEYSFSITDYIK
                     SCGKIPGTSEFNLERINADTDLSNKLSVELNSPVIKISRLRTSDGVPFSYDINLIPSK
                     YLPQKISRKIIGESLFTFIERNIDPKICYFSTSYKPLISDEFISKKLAIKKGTPIIKN
                     TQVHYSEETKPLWCLEIWIQEYVGVLHYISPPNKTIET"
     gene            6749..8092
                     /locus_tag="PLL88_00440"
     CDS             6749..8092
                     /locus_tag="PLL88_00440"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOJ00056.1"
                     /translation="MSYEKLATELVEALGGAENISTITNCATRLRFALNDNTKYDEEK
                     AKNIKGVIGVSRFGKQIQVVIGPQVNEVYKEIAALDSVKKALSSSRIKVKKAWMDIIF
                     GYITNCIIPIYPAFIAGGLLKGILVLATNLKWISGTSAIYSVLYAASDAVFYFLPVLI
                     AYSAAKYLKSNQVIAMIVALCFVMPKYTGILGTDIINKAFLVSYSSQLFPMLVAIPLA
                     SFVEKKLTEVIPNTLKSIIVPTVTVIVTVLAVFFCIGPITSFVGNVIYKAVNAIYAFS
                     PLLCGTFVGGVSGYLTIIGAHLALIPIIILNITETASDWLLPMMQMTCMAQIAIAFAY
                     FAAIKDRTSEKKAAALTAGLTGLLSNITEPALFGFVVENKMMLIYMGIAGAIGGALVG
                     ATGIVYSTIMSGIMTWIAASPTLTMNLLFDAFRLLDMAIAFVLAFTYLKKKGVEG"
     gene            8549..9565
                     /locus_tag="PLL88_00445"
     CDS             8549..9565
                     /locus_tag="PLL88_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOJ00057.1"
                     /translation="MNNRLMIKETFLKFVSIMERGTVDELREILTEDVILMTSNYGNA
                     KGIENVIKKLRWNGMPVDFSRSRIFNFVVQNDENIAKQSAVVTTLVGNNDPDYFHYFH
                     FAGYYLNDLVKINDQWKISYIRFNLDMEDGNTLFVRGWWRLIDYRYFEGTAYYPIVSE
                     IDAPWRAIRNPEPILDEKEQILDTFNRLNWGIDHADFELAISSYDTENGDFSGMANVM
                     KEQNLIPEWDEAGIRDVVRLLKYKRYKEAIMEHIWKIHKLNIDGTRAEMTVYRYEPHR
                     LGTTKFNKLNMHYDFFTTVLDYEFVKRDGDWRNGGGWKITKEVPRRGRIISEVTYDKE
                     KFYE"
     gene            9540..10193
                     /locus_tag="PLL88_00450"
     CDS             9540..10193
                     /locus_tag="PLL88_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00058.1"
                     /translation="MIKKSFTNNRNEIYDLLFEEDSQIVIYSYQKEDLNDDQSTNLEN
                     LFLGIISEIDCLISELIKEPQLAVLLFKRNNVDCLLIADRNSALLQMAIYKYESIDDI
                     CCENLFVSEICLEKHEPSEGKKALYFFVYFFFGDFNHMVSLLDDHVEILKHGKRYRGK
                     CSAIMILKSLRFSKSIRESDCFKINSTFDDYIFEFGSQIIRIPNTDDNIKEIFLEEG"
     gene            10232..10672
                     /locus_tag="PLL88_00455"
     CDS             10232..10672
                     /locus_tag="PLL88_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HOJ00059.1"
                     /translation="MINIFVPCTGDIVDVTTINDEVFKNKMLGDGMAVIPNGSLVFAP
                     IDGKIVSIFPSNHQFTMKTDDGLELLIHIGIDTYDVKKRFLVRLVEENQYVMRGTPVI
                     KAKFNKLRAKGYDTSVIVVVLNHPIFDKTKMSKSESIDTVLFSI"
     gene            10701..12059
                     /locus_tag="PLL88_00460"
     CDS             10701..12059
                     /locus_tag="PLL88_00460"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOJ00060.1"
                     /translation="MVKKLKIATIGGGSSYTPELIEGFIKRKDKISISELWLVDIEEG
                     REKLEIVSGLAERMVKANNLDWKIYATLDRREALKNADYVTTQYRVGLLDARIKDERI
                     PISYNMIGQETNGAGGIFNALRAIPVIKDVIDDMKDLCPDAWLINFANPSGMVTEAAI
                     NYCGWERAIGLCNVPIFMERKESEALGLSNDDDLCFKFAGLNHYNYHKIWNREGEDIT
                     EQLIKLFYSPDSTANPEAIKNISDFKFNFSSLKGTGYLPSPYLRYYYLTNEMLEEDLE
                     VYKNHNVRGEEVKRIEDTLFEMYKDPKLNHKPELLSKRGGAFYSDVACEVITSIINDS
                     RKLMSVNTKNKGSITDLDYDAVVEVTSVITSHGPEPISWGPFTPIAKGLLQNLKAMEI
                     LTVEAALEGNYEKLVQAFILCPIITSGGKAIDMLNEMLVANKKYLPNFAEKIEEIEAA
                     KR"
     gene            12549..12947
                     /locus_tag="PLL88_00465"
     CDS             12549..12947
                     /locus_tag="PLL88_00465"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HOJ00061.1"
                     /translation="MQEPKNQFHTIDEYIAGFPEEVQKLLKELRATIKAAAPEAEEKI
                     SYQMPAFAQNGVLVYFAAHKQHIGFYPTSSGIENFRKELSAYVTSKGAIQIPYSKPIP
                     YDLVNRIVTFRVQENLKKAENKGTNRKKGA"
     gene            13010..15073
                     /locus_tag="PLL88_00470"
     CDS             13010..15073
                     /locus_tag="PLL88_00470"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOJ00062.1"
                     /translation="MKKMKLNGEVRTALVYFVLGCIWIFLTDHFLALLITDSSTLTDV
                     QSYKGWFFVAASTFLIYFLLKRYLASKRRAELKQIESEEQYRLLFETSLDAVFLTSPN
                     GKILAANPAACKLFGRSEKEICNLGRNALVDTSDPRLAPALEERMKNGYFSGVLTFKR
                     NDGTKFEGEISTALFNDSNGNLRTSMILRDITDRKRAEEVIKKKQQELQHFIEHVPAA
                     IAMFDKEMRYLCVSQQFFSDYRIEERDIIHKSHYEIFPEISENVKEIHRRCLKGETIS
                     NDEEPFPRLDGRVDWVRWEVLPWYENNGEIGGIILFSEVITKRKLVEERIKTQLYRLK
                     TLREIDQAIVASNDIHVVLNTLIEMLLSSLRVDAVDVLLLDPATNTLTFAVEKGFLTP
                     KLEKFSVHLGTGLLGQIALGRERLFVPNLRETSQQLSRPWLLTDEKFVSYGCAPLFIK
                     GELKGVLEVFHRSLLNPDAEWLDFLDSLAGQAAIAIDNARVNEGLRSANLDLTLAYDA
                     TLEGWSRALDMRDKETEGHTERVTQFTLEFAKRLGISEEERVHIRRGALLHDIGKLGI
                     PDHILLKPAELTEEEWEIMRKHPVYAFGMLAPIAYLRPAMDIPYCHHEKWDGSGYPRG
                     LKGEQIPLAARLFAVVDVWDALRSERPYRKAWPEEKALEYIRAQSGSHFDPKVVEVFL
                     QMMAE"
     gene            15242..16111
                     /locus_tag="PLL88_00475"
     CDS             15242..16111
                     /locus_tag="PLL88_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HOJ00063.1"
                     /translation="MRNEKEMFDLISQTAQQDERIRAVILEGSRANPHASKDILQDYD
                     IVYIVSEMELFVRDHEWVKCFGELLIMQIPDSMGDQPHSTEDHFAYLMQFRDGTRIDL
                     SLYTLEAFQKRGRDSLSVLLLDKDTTFESFPVADESDYLPHPPTSKAFDDCCNEFWWV
                     CPYVAKGLWRGEITYAKYMLDVIVRAQLMKLLNWYIGMRTEFKCNPGKEGKYFQKYLS
                     TEEWEKLMRTYADADPENNWRALFNMADLFRVIAQQVAEHFALDYPNAEYQHVLVYLK
                     QIQNMPKDKKELF"
     gene            16199..16675
                     /locus_tag="PLL88_00480"
     CDS             16199..16675
                     /locus_tag="PLL88_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ00064.1"
                     /translation="MTNVLTLRNVKQEDLAVFFANQMDEDADFMAAFTSQDRFDKNAF
                     DAHWNKIMHDPTVHIRTILVDDGVAGYVLSYEEEGRPEVSYWLGKDYWGRGLATGALR
                     IFLQQVNATRPIYARTAKDNQASRRVLEKCGFKVIAESEGFSNARGKKIPELLLVL"
     gene            complement(16832..19273)
                     /locus_tag="PLL88_00485"
     CDS             complement(16832..19273)
                     /locus_tag="PLL88_00485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOJ00065.1"
                     /translation="MSKTDKRYSSGLTKAEVLERRRQFGENRLPAEKSTPPWVLLLNQ
                     FKSPLIYIILVAALVSLLANETSDFAIIIAVVIVDVILGFIQEYQAQKTYTALKGLLK
                     PTTTVIRAGERCEVEVWELVPGDLVILNAGEKAPGDGVLLESVRVTIDEAILTGESEP
                     VTKVANDQVFMGTTVLSGRGLMEVSGTGSSTELGKIAASLQEHVEEDTPLQARLKAFS
                     KTLTYVVIGFTLTILIAGLALGGSFLDTLRTSIILAIAAVPEGLLIAVTVILVLGMRK
                     IFKRNGLVKRMQAVETLGSVTVICTDKTGTLTEGRMRVTRADLTDQTRAYQTMVLCND
                     LEGPVDVALWEYSLTLASDDPQKLVDRTERLSEEPFSSETKFMVTVNNLDGKKYNYLK
                     GAPEIVLEMCKLEKKEKERILVRVEEWAGDGLRLLGLAYRGGGEPEDHSGYTWLGLLG
                     MEDPLREQVQESIRVAQKAGIRVKMITGDYRVTAERIARTIGLMHAGDEIIEGTDLEA
                     MSDEELAERVKKTAIFARIRPHDKLRIVRALQNNQEVTAMIGDGVNDAPALQQANIGV
                     VVGSATDVAKETADLILLDNNFRTIVAAIEEGRIIFENIRKVVAYTLSNSFAEVLTIF
                     IAMMLRWPAPLVVAQILWIHLICDGPSDIVLGYEPKERGIMDEKPRSLKEPILNNLGL
                     SLIGIISTASAAAALYLFNHMYTVHLNVVEGRSIVFASFAINSMIYIFAYRSMRIPLF
                     RMNKVTSNKPLLWAVGAGLVTAILPFLIPAIGNLLGIVPLTLQDWLMVAGIAAGLLAV
                     VEVGKFISNKLHAKD"
     gene            complement(19257..19562)
                     /locus_tag="PLL88_00490"
     CDS             complement(19257..19562)
                     /locus_tag="PLL88_00490"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ00066.1"
                     /translation="MNANSFILDEHTAAHVAELFSVFSDTSRVRILSVIVEQELNSQA
                     LATLIGVTESAVSHHMRGLRQMHIVQARRDGKEVYYSVVDPHIIALFKQGIDHVKNG"
     gene            19708..20007
                     /locus_tag="PLL88_00495"
     CDS             19708..20007
                     /locus_tag="PLL88_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00067.1"
                     /translation="MYGQSIEVASHPDIQLRHAAYLVVCPEGVQYSAMNGGRANERIQ
                     TSLCLGSTVETEIEEKQATLIVVDNDPALDHILPDGGAIRLSFDPVRCYLLPYET"
     gene            20257..20649
                     /locus_tag="PLL88_00500"
     CDS             20257..20649
                     /locus_tag="PLL88_00500"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ00068.1"
                     /translation="MKGFIQNIEDITVKNENFRQVLYTAQNCQLVVMSLNPQEEIGME
                     IHHLDQFFRVEEGSGEAILDGVTTPISAGFAVIVPAGANHNIINTGNVPLKLYTIYAP
                     PHHRDGVIHHTRAEAEHDEEEFDGRTTE"
     gene            20913..21479
                     /locus_tag="PLL88_00505"
     CDS             20913..21479
                     /locus_tag="PLL88_00505"
                     /inference="COORDINATES: protein motif:HMM:NF022167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase"
                     /protein_id="HOJ00069.1"
                     /translation="MNEPEPTIKENKTEMTAQDVLAFLSLMKQNNIEIWIDGGWAVDA
                     LLGSQSRLHEDLDIAVIHRSVGRIRALLEASGFSEIERDDSWLCNFVMGDEQGRLIDV
                     HSCEFDPEGRCIFGVHYPYDALKGSGMIGSVPVQCITPEWLVKFHIGYTLDENDYRDV
                     KALCDKFHLEIPKEYADFGKVEEGKRKD"
     gene            21483..21947
                     /locus_tag="PLL88_00510"
     CDS             21483..21947
                     /locus_tag="PLL88_00510"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOJ00070.1"
                     /translation="MSKQHTFKAKIEDAGTGGAFVTVPLDVEAVFGKSRVKVKAWIDG
                     EPYRGSLVRMGGQAHILGIRKNIRTRIGKSIGDVVEIILQEDTEPRMVTVPEDLQNAF
                     AGHKDAWAFFDTLSYSHQKEYVQWIEEAKKAETRQDRVQKTIRFLLEKKSRQ"
     gene            21984..22472
                     /locus_tag="PLL88_00515"
     CDS             21984..22472
                     /locus_tag="PLL88_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00071.1"
                     /translation="MIDILAGDEPFPAYKKDLMLYGQLVGSWSVQSTWYDRNGEIKKK
                     RGEWHFGWVLGGRGIQDVLFAEGATPDQYGTTLRCYDPALKIWHVSWMQPYGGEFVHL
                     IGRKVGDSIVQEGSGLDPARRERWTFRDITANSFLWLGEVSFDGGKTWFLEQEMRGTR
                     ME"
     gene            complement(22501..23109)
                     /locus_tag="PLL88_00520"
     CDS             complement(22501..23109)
                     /locus_tag="PLL88_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00072.1"
                     /translation="MLTREKIESYPKEDLPALALTLTKGDIAQLVPWLSEKADTLRYH
                     CFLLLLARSEQYEDVFPYWDTFVTKLSDPNSYQRNIGLRMIAENVRWDKQGKFNEICD
                     LYLSFCDDEKPVTVRQCIQGLCSIVPFNTHCHARITDKLTSIDISQRKESQQKLVLLD
                     IISVLLLINKANSQNDILNYIHHALSGGLLDKKSKLAIEAQL"
     gene            23367..23696
                     /gene="cutA"
                     /locus_tag="PLL88_00525"
     CDS             23367..23696
                     /gene="cutA"
                     /locus_tag="PLL88_00525"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HOJ00073.1"
                     /translation="MKTNELIIVQTAAADQDEAEKIATLLVSQKLAACVQMLPIQSCY
                     RWKGKVVHDSEVLLLIKTRSDLYEELEAAILETHSYEVPEIVQVPIERGSANYLQWIV
                     ASTRKEA"
     gene            23697..24374
                     /locus_tag="PLL88_00530"
     CDS             23697..24374
                     /locus_tag="PLL88_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00074.1"
                     /translation="MDAIRVFLESASHRVFCGALDWPGWCRSGRDEDAALQALLAYAP
                     RYAEVLQIGNVWFPEVKNLSQLIMAERVPGNVTTDFGAPDAILAGDQEALDEKETARF
                     QAILWACWRKLEQVARAAEGKILRSGPRGGGRDLQSILEHVMESNAAYLARIAHKVQR
                     SAQDDLLAELRTIQQADQTAIENAIRNGLPKSGPRGGRIWTLRFFIRHVAWHVLDHAW
                     EIEDRVE"
     gene            24556..25167
                     /locus_tag="PLL88_00535"
     CDS             24556..25167
                     /locus_tag="PLL88_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOJ00075.1"
                     /translation="MKAIALVVSARKQGNCYDFAQFVLDDYKNKGVETELINFYDYQI
                     TPCQRCAYECIQKLDPAKGTNSACPIQDDVRMIWEKTWQAEILLLFIPNYGGMPPALW
                     LAFSQRSQAFFRQAPVEKLKHSVVSALIVASPHQSSGAAWAFSFMADEVKGLDRKVAS
                     FEVINPPQFQDEYTFSPLMQNAEIQRRLKFMAEQTWKTAVALN"
     gene            25207..26361
                     /locus_tag="PLL88_00540"
     CDS             25207..26361
                     /locus_tag="PLL88_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOJ00076.1"
                     /translation="MKKWMPILVIGSILVFTAGVIYFYAHSSSEEDVTNEDGSQTPSF
                     ESIGKTTPANQSELPVLLAYYTGDEGSFKALQMAAPYLTAVSADVYLVQADGSINGSD
                     EMSAMTWNDARNIETYACISNYNDDPEVNDFDAELAHAAIVTYREQLIESLVSVARDG
                     GYAGINIDFENLAYSADIEEDRSAFTSFIQGLAERLHAESLKLIISVPGKSEESIDNT
                     WAYPFDLAVLGETADYLQLMTYDQHGPWGEPGAVAGADWVEDCLQYATSLVDPAKLLI
                     GLPAYGYDWDLTASDFENGSIVAGDISWKDFAALVSRPDVETGWDIASQSPYCRYSEE
                     GHAHIVWFENRESVQTKVKLVREYALAGVSVWALGKENENFWQAVEEGLK"
     gene            26639..28639
                     /locus_tag="PLL88_00545"
     CDS             26639..28639
                     /locus_tag="PLL88_00545"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ00077.1"
                     /translation="MNKYYPNLFKPLKVNSLMLKNRIFSSSMGMPAHHILLSSTYYDE
                     VSLLDKAMGGAALVFLQPEKAIADDNCEYPKYERDRIREDLSVARQYGAKAAIQVWPR
                     LKINTGDSLRAHYDGMVVHAPSPYLTKSGQRAIELTKEEIKMMIQEAVKDAIAAKNFG
                     FDMIQVRVGAESIAAQFLSPAFNKRTDEYGGSLENRMRFSIEYIRAVREAVGADYPIM
                     FHVGETQFLPKSYGAEDVYALLDNVKEYVDMINAVAGGMDMIPGYFPEDKTFDPAMGF
                     KAWYAANGVATSKFTSTESLSLIFAAKVKERYPDKIVITNGSIMTPETAEAAIAEGKV
                     DGIMQGRALNADPFWPRKAMAGQREDIVPCLRCGYCYHTATNHTNTQCSVNPRYRREN
                     RVPLEVPKAKLKKKVVVVGAGPAGCKAALVAQERGHEVILLEKSSEIGGQIRYGQYEK
                     IKVDLVNYLNYLKAQIKKNNIDIRYNTTATPELIRELKPDALFVAIGARPITPDIKGS
                     NGEHVHQVLDIYPQLARLGKRVTVIGGGLVGCELAYELLEKGHEVTIVEMTDQIAAQG
                     HFLYRMDLERGLEKHGDRVTILKNTVCKEIQGTKVLVEQGGEKKEIEGDDVVIAVGMK
                     ADKEEAFAFYGIADETFMVGDCEKVGKVLEATNDAYFIAANL"
     gene            28835..30244
                     /locus_tag="PLL88_00550"
     CDS             28835..30244
                     /locus_tag="PLL88_00550"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOJ00078.1"
                     /translation="MTKLNKNFLWGCASAANQCEGAYLEDGKGLSLADCLTAGTKERR
                     REYTDGVEEGKYYPSHEAVDFYHHFREDIKLFAEMGMKCFRTSIAWSRIYPHGDDDEP
                     NEKGLQFYDDMLDELLNNGIEPVLTITHYEPPYALVEKYGSWKDRRCIDLYEKYCRTI
                     FTRYKDKVKYWMTFNEINSVIFNPTFPVGIRILPGENKKEIAYQAAHNMLVASARAVR
                     LGHEISPDFRIGCMICMPMFYPETCKPEDQVEAMKANDDTYFFSDVHVRGLYSRKTKR
                     LFEKNDLHINMETTDERDLEEGTVDFIGFSYYSSAVSTSREVDKSQGNIFWMVKNPYL
                     EESEWGWGIDPQGLRIACNCLYDRYQIPLFCVENGFGAQDTVVNGRVHDPYRIDYLRK
                     NISALIQAIDEDGVDLIGYTVWSATDIVSAGTGEMRKRYGMIYVDRDDSGGGSMQRIK
                     KDSFYWYQKVIASNGDDLE"
     gene            30433..31692
                     /locus_tag="PLL88_00555"
     CDS             30433..31692
                     /locus_tag="PLL88_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOJ00079.1"
                     /translation="MEKFTKFLEKYLEPVAEWISNNRFLKSISNGMIMLMPFLLVTAL
                     FSIVQGVPAWLDFIPDWSEGVSNILLLPYNVTFGLIALIISFTVAYNHAEKLKINPAT
                     GGLISLVVFVILSAKYENGAFDGTFLGSRGIFTAILVALLVIELYKFLLKLNLKFKLP
                     ESVAHTVQSSFEAIIPLAVILVVFYGLSVLVQTTTGQMIPALIQSAVAPALKASDSAL
                     FWFVLGILMSVFFWMGMHGWAVMSGVWLAATIAFTSANAEAAAAGQPMPYISGGTIWL
                     CGQLHWHVPLMLMLFCKSERNKAIGKATLIPGIFALSEPYTFGVPVAFNPILAIPYIF
                     AGPIANVLNFYAVKWGLMNRSANLIQILAPAPLQGFLGTNGDWRVFPIFLVLLAVEVV
                     IWYPFLKVWDNKCLREEKEAVASAKTK"
     gene            31725..32027
                     /locus_tag="PLL88_00560"
     CDS             31725..32027
                     /locus_tag="PLL88_00560"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00080.1"
                     /translation="MKTIILFCANGFSTIMMRDKIRETSQNEGLDYTIEAFPFAKLEE
                     EGQRADLILLAPQIRFNRKAVEKLYPDKKIIIVDMAAYGAMNGSAVLKEIRNTLGD"
     gene            32051..32365
                     /locus_tag="PLL88_00565"
     CDS             32051..32365
                     /locus_tag="PLL88_00565"
                     /inference="COORDINATES: protein motif:HMM:NF014326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HOJ00081.1"
                     /translation="MKEEITNISCEIIALAGSAKSAFIESIRAAKDGDLNKAKELFDI
                     GSGQFLQAHEIHANLIKRFADGDEIEMNILLVHAECLLTSAEDFKVISREIVEFAERN
                     KK"
     gene            32490..34391
                     /locus_tag="PLL88_00570"
     CDS             32490..34391
                     /locus_tag="PLL88_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ00082.1"
                     /translation="MNLTVRQYQIIRLLKENISALSGNQLCSELDVSSRTLRYEISRI
                     NKYLGCDLIRSSREGYSIDASLDLSTIFNDIRLNDYLYIVKSVAILCLEEKDLSVYDI
                     EDQCFVSKPVITNMLHLILHQMNKFDLQLYRTGDFISVKGTEFNKRRYLINLLFSETF
                     DESTFCPESKNYFTGFELSDLWELVTSTIEDIGIHINDVYLKHIVNCTAVSLQRIMNG
                     HITEKHAAAVVNFGEEHEAYLFLTSLLDKVFHKYKVKVLEEDFRSMLCFYAGSFRDTN
                     ANPLRMTILNNKEFEITIRRIMDRTLKQFNLHANYEPFFDRLLLHVYFLLLRAKTSSF
                     VKNDITSTLKQTNPFIYDVSVFMAYLIQMEFKIEIPDEEIGFLAIYFGSVIINTINYS
                     NSYTVICVCPNYNNLREQIEAQLLSMFNKRINIVKYVSSYSHISDDDTYDFIISVISN
                     SYTFKNIVYVSPVIGPWEYQKINAMIIECEKKRQKEEIKALVLKYFQPDLFFYNEPYL
                     KDMEIMQFLHSRMKDKGIVEDDFLDYVLKREELASTAFFNKFAIPHAINANARDTKVA
                     YYYSDKPIRWFGSDVNLVLLMATKQYDKNFIRLYELIFAILMDKELYCKLLKCNSYEK
                     MVDFISNQL"
     gene            34454..35035
                     /locus_tag="PLL88_00575"
     CDS             34454..35035
                     /locus_tag="PLL88_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HOJ00083.1"
                     /translation="MELTKTLYVPDRTAWHAWLEEHAASEREVWLIYYKKDSGKPRIP
                     YEEAVEEALCFGWIDSLVQRIDEERYAQKFTPRKLGSKWSELNKRRIAKLVKEGRMTP
                     AGMAKVDFHVDEINLDEPVISGKKPMPELSPSIQQLFMEDNVVWENFSKLTPSHRRNY
                     IRWIMDAKKEETRMKRAQEALELLKQNRKLGMK"
     gene            complement(35159..36148)
                     /locus_tag="PLL88_00580"
     CDS             complement(35159..36148)
                     /locus_tag="PLL88_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00084.1"
                     /translation="MNSIKKILYLLFLGAVLLFAGCKPAAQINAHTVADLVSAEVVQS
                     AEALNVRWALDGKSFWLQGLTRATLYNSKTYQEIASYEVGEYATLYSVSPDGKLVAYA
                     SDEPSIVLYDVLAQQEVVSIPQEVFVQQAAFSPDGKWLGIASSEEWQITIWDIASAGI
                     IHKLTGFTTAAPVYSFQFGADGKTVLWIARATIQPMDIDTATLGPEISHEDFISASAL
                     SPDGALLATAAAGSVDGEFLPIITLWDAHSGEALAVFSNPDYFSSLNFSPDGSLLAAG
                     SDGKVLFWDVVNSQFIGEIHTASGFVNSLQFAPDGTRLLTCTNEGTVQMWQVR"
     gene            complement(36196..37806)
                     /locus_tag="PLL88_00585"
     CDS             complement(36196..37806)
                     /locus_tag="PLL88_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00085.1"
                     /translation="MKKFFSFLLVIALLAAVIYVFLHKGSNALTSPSPIGLDMSAYLE
                     NAQDGEQFVDTATTLGVTSVKIAIPWQFVERSAGLFTWSINSQGTQIDLGDLIHKLER
                     EGMSITLVLEGFPSYLQYENLSEENVASLYLESWERYVRASVAEFGDVVDAWQIGESV
                     NLPFELQDQPIAAAVLASPSTYAERLAIASNAIKEASHSDLVILGGIRSDTGNCINQP
                     SAFLNSINTLGMWNAFDVIGVELDTYSIAPEGKYMYQTYDTVSGVCLTSAEGGFTLAD
                     IIALVDAAGSQYGEKPIWITGLAWQTEDLAAIAEERGTLVDVVRADLLSRASIMLLGR
                     HNVEKIFWQYAETDSAADNSFGVFSQQVFKNLAASLQGLRSSQDASDIFNGYYQYRMT
                     SAGMVDIFVWRGYGGDDFLAYTLQNVGGYQLEAFSLDADSIRSGKGLELSVSTNGETA
                     FMLSERPVLIKATPTDLKERLALSMQGLLTSAGKTIKNSAEDIVEEQKEKASQELEEW
                     LDEKKESLFAMLKESFMEWLDKILNWEQ"
     gene            37921..38805
                     /locus_tag="PLL88_00590"
     CDS             37921..38805
                     /locus_tag="PLL88_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00086.1"
                     /translation="MADEKNEPLSRSVPRFAVLQLSGCAGCEVSLLNAHEWTENFQLA
                     YMPLVSSAYAVPEVDILLISGGVRTDEDAYHLKNAVKNAKKVVAVGTCAISGGVANLG
                     NRDEVRQLFLRRQDRLEVPRLLPRSRPVDELVPIDLYLPGCPPTPELFMAALTDPEGY
                     QASLIVCQECGRKKLKEPKPDHLLGFQTGKVDPEICLINQGYACIGTSTRGGCRAPCT
                     RAGHPCVGCRGPSNLLIEKDSQAWLESIQKIFHGMTNVSDEELREALLSPQLALFLFQ
                     FSDYAGGDRPARSKEKVL"
     gene            38805..40118
                     /locus_tag="PLL88_00595"
     CDS             38805..40118
                     /locus_tag="PLL88_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOJ00087.1"
                     /translation="MATLTFPLSRIEGHARVEIEVQNGEVVSTRFKATEMRGFDIFVR
                     GVPAEQMPVIVPRICGVCSTAHHVASVKALEDAYGVTPPPLASRIRSLLLLGQLLQNQ
                     ATSLFIFTMPDRVGVDSLFEMDDVQGNTAKRFEMAKQALKIRELGTDLITLAGGQFIH
                     PVRAVVGGITAGISAANAEPMLKRLEQALPTACDLFDAYWELTIRMKDRIGSWGDDQP
                     AYYITSTGKVKPDFNSHEIRVMNPAGECCEIFSPRSFRDVLEFKESEYSYAGTTSYKG
                     GVIRANSLARANLTAHMGTRVANGYLHRLRIEFGTPAHPIMLFDLGRGIELVYAIERA
                     AQMLAQPLDNEDTDVPYTPRDGEGYGLVEAPRGPLIHHYVIEKDKIAKAEFIIPTVHN
                     MHSIERALQVAAKRYISGDAVDMELERAVGRVVRAFDPCIACATH"
     gene            40207..40587
                     /locus_tag="PLL88_00600"
     CDS             40207..40587
                     /locus_tag="PLL88_00600"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HOJ00088.1"
                     /translation="MYYSDWEPSFGFLFAYVLFVLALVGFYLYCTWRLFEKTGRKGWE
                     GLVPGHDTVEMFEIVGRPGWWFFLLLIPIANIVIGIMIQFELAKVFGKDTGFGFGLLF
                     LPFIFLPILALGGAEYQGPLTHEA"
     gene            complement(40800..41219)
                     /locus_tag="PLL88_00605"
     CDS             complement(40800..41219)
                     /locus_tag="PLL88_00605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ00089.1"
                     /translation="MKNYRKELWFDVPARRAYQNITPQVEACLRESGIQEGLCLVNAM
                     HITASVYINDDEDGLLHDYDTWLEKLAPHDPVSGYQHNRTGEDNADAHMKRQLMGREV
                     VVAVTRGKLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            41370..41900
                     /locus_tag="PLL88_00610"
     CDS             41370..41900
                     /locus_tag="PLL88_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00090.1"
                     /translation="MDNEAVFRVRMPEVDIRPGLEAIFTQARELAEGETTLADGSHLR
                     RVVIVSPGRLLLIKDSFPPDTMPLELRLALEELLPSDRALKIAVIAYTDLDALRKDIQ
                     RAIPFFDHLLGFAYLGHAVWIFEGHISVLEMACLGADYVLVDQRMLPFLDPDWEQIVR
                     LRCGVANIRVLSIPEE"
     gene            complement(41949..42653)
                     /locus_tag="PLL88_00615"
     CDS             complement(41949..42653)
                     /locus_tag="PLL88_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00091.1"
                     /translation="MKKKFALLIAVLLILIVCVPVQAATYPTFDIMSVVVDESVTIRT
                     NLFPANELYYVYMGYNGTLGINGILVTKIWTGSGGTFKAKFYIPDELKGEDIVAIRLE
                     SPTSPYYSYNFFYNTTSYTANYSTSSSSSTTTYAPSDFPTFNILSVVKGESVTVETKY
                     FPAGDMFGVYMKDGNSAAKTWYYVAGFNSADGASFQATFAIPSQLYYKEKIAIKMYNL
                     DEKYITYNLFENKNYP"
     gene            complement(42800..43915)
                     /locus_tag="PLL88_00620"
     CDS             complement(42800..43915)
                     /locus_tag="PLL88_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HOJ00092.1"
                     /translation="MTYPKNYTVVGAGNGGKAMAAHLALKGKDVTLFNRTFSHIEVIA
                     KRKGIELESYPGGPQGFAVIKKATSDIAEALESAEVIMVVIPSSGHADVARAIAPYVK
                     DGQIILLNPGRTFGAIEFDTAMRREGCTANAIVAETETFIYASRSDGPAQARIFRIKE
                     AVPVAALPANRTDLVLQAIEDVYPEFIHGTDVLHTGLNNMGAIFHPALTILNAGRIES
                     THGDFEFYIDGVTPSVGYVLEALDRERVTVASAVGIRARTAQEWLELAYNVQGKDLHE
                     AIHNQSGYYGIKAPSTLSHRYIFEDVPMSLVPIASLGKQYGISVRGIESMIRLACIIH
                     KTDYYRTGRTVEKLGIKDLSIAELNTYVKERLLHNLA"
     gene            complement(43932..45560)
                     /locus_tag="PLL88_00625"
     CDS             complement(43932..45560)
                     /locus_tag="PLL88_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOJ00093.1"
                     /translation="MKTVVAGALGECVHVAGVYNFLRLAEQCGWKTIFLGPAVPSEKI
                     LQTAREEKADLIAVSYRLTPETGERLLAEFAEAADDLHSQGIQFAFGGTAPVAEKAKK
                     LGFFNAVFDGTQSREEIVAFLRGDPHTESDALIYPQSAVERILWKRPFPLLRHHYGQP
                     TVRATVEGVEQIADAKALDVISLGVDQDAQENFFHPGKQNPRATGAGGVPVRSPEDYA
                     RLYKASRTGNYPLMRTYSGTNDLIRLASMYCETIHICWAAVPLFWYNQMDGRGPFDLE
                     AGIREHQNLIRWYSEHNVPVELNEPHHWGMRDGPDVIYVVAAYLSAYNAKHLGVHDYI
                     AQMMFNSPTGTSESMDLAKMLACLELCATLESDGFHLWKQTRTGLLSYPLEINAARAH
                     LAASIYLQMALKPDIVHIVGHTEAHHAATAQDVIEASLMAQRVIENALQGQPDMLEDP
                     AIQKRKEYLVHEAQTTLQAIHNIGAESAADPLTDAAVLAKAVTKGILDAPQLLNNKFG
                     RGKIKTTIINGACEIVDDNGNPQSEEQRLAQFLQ"
     gene            45715..46467
                     /locus_tag="PLL88_00630"
     CDS             45715..46467
                     /locus_tag="PLL88_00630"
                     /inference="COORDINATES: protein motif:HMM:NF018757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOJ00094.1"
                     /translation="MGSIKVLLAGESWVSNSTHYKGWDYFSSTVYETGEGYIRSAFVE
                     AGIDYTHLPGHLAAEKFPLSVRELENYDVIILSDLGANTLLLHPDTWLHGKSVPNRLR
                     LLADWVSEGGALIMCGGYYSFAGIYGAAKYYRSPIEEILPVKIYPFDDRVECPEGLTA
                     EVLMPSHPLVNGMEGDWPLLLGLNEMEVRAEADLIVRAGKYPLLAAWDYGKGRSMIWA
                     SDIGPHWCTVEFAEWQGYTTLWKNAAHWLSKK"
     gene            46820..47134
                     /locus_tag="PLL88_00635"
     CDS             46820..47134
                     /locus_tag="PLL88_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00095.1"
                     /translation="MKERYLFYQLNRCTGCQLCIMACALARRGVCGENESLIKILTHP
                     QFGTSQPLVDQSCEWEECDARCVQVCAPGVLHLAEKKEWGSLMTKQGWTPVPVLVMEK
                     EK"
     gene            47131..49023
                     /locus_tag="PLL88_00640"
     CDS             47131..49023
                     /locus_tag="PLL88_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOJ00096.1"
                     /translation="MSGGYCNQVAWIDLSSKSVYTKPLDGEIKERYIGGRGFITRWLM
                     DMVPPSVQPFDPGNALIFATGPLTGTNVPTSSRIEIGAVAPETGIFSSGSAGGSFGTK
                     LKRAGFDALVITGRSEKPVYIIVRDNHVEIKDARRYWGRDVFETDRAIKADHRDSRLS
                     MACIGQAGENEVLITTIMIDRVRAAGRGGLGAVMGNKRLKAIAVYGSGSVPIHAPEPF
                     WNACEIIREAAVKQFFVKRWENGTYGNLTRYQGVGALTSYNSRKTTFEYIDRISATHY
                     NENYRVGRRACVSCPMPCWTTFVIQSGEFKGFFGEETTATTFKEMGARCGLHEMDAIL
                     KAHSDLNRYGLDTISTPAVIAFAMECYEEGVITKEDTGGLELTWGNRHAVIPLIEQIA
                     GNKGFGAQLARGVRICAQTWGEKTMKYALHVKGLETVGTDPRGQPSWGLGYATSSRGA
                     CHMRAYANYESGVNNAPGGMDEKTMIRISGTTEIGKRFSTTGKGKAGAFLEDMNAFGD
                     SLGICKLMSRAELGLPESLVDVLNYATGADYDTEGLYRVGERISNLERIANLQRGITP
                     ADDTLPERYLHEPVPEGPAAGQVCDLEPMLNEYYETRDWDRADGYPSTHKLNELKLDR
                     MGKDIE"
     gene            49269..50270
                     /locus_tag="PLL88_00645"
     CDS             49269..50270
                     /locus_tag="PLL88_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ00097.1"
                     /translation="MKRNIQFDCPERIGLRFNSLGVSDVFRIFVQPPQRCRPADAQNV
                     RMDKKIRATPGQADEWGCIWESLEEGSGDMGQVLHPPMKSLDEVDSYPFPDPHDPCHF
                     DGLEEALQLSNDKFVQLNSPFCLFERMHFLYGFSETLRDLALEPQKMESFIDKVIDYQ
                     IGIAEMAGKLGKGRIHCFDTTDDWGTQTNMFISPKMWRALFKPRYKKLIDAIHANGMV
                     IRFHSDGRINAIFPDLIELGVDILNFHQPLLVGIDEVAGQLTGKVCFEVSADVQQTLP
                     TCDEQKIKNEIKEIVQKWCTPKGGLIGIEYRYGKVLGISEQALRWELEAFQQYGNFK"
     gene            50294..51088
                     /locus_tag="PLL88_00650"
     CDS             50294..51088
                     /locus_tag="PLL88_00650"
                     /inference="COORDINATES: protein motif:HMM:NF019003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HOJ00098.1"
                     /translation="MELLEKEGPAYERRSQRFQSWYESVYKWHPNFVFKPNAKINWSA
                     ITKPMSEWKVGLVSSSGTHLRSQIPFDIVSDDGDWSYREIPVDTDPKDLMVSDAHYDH
                     SDADSDVNCMFPIITLRELVKEKVIGSLPTRFYGFMGFVPDPTELIAHNAPEVAQKLA
                     DEKVDVVILTPGCAVCHQTLGIIQNIIEGAGIATIATTLKPELTEQIHVPRAAYLKYP
                     YGYSAGPANDLEMQKKIIKEVLELVPAIKEPGTIVKMPYRWHGRFE"
     gene            51104..51364
                     /locus_tag="PLL88_00655"
     CDS             51104..51364
                     /locus_tag="PLL88_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00099.1"
                     /translation="MAVDKRTDQLMAHVEAMLEIIDDMNQDMQSRIEEENASDAPNDS
                     RINFYTSQQRRLVKLTDLLEEDILEVLISIRNISGPIEYFEN"
     gene            complement(51444..52160)
                     /locus_tag="PLL88_00660"
     CDS             complement(51444..52160)
                     /locus_tag="PLL88_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00100.1"
                     /translation="MAPLLQVNSIKAYYGNICALKGVTLEVNQGEIVCLIGSNGAGKT
                     TTLKSITGLLHPQEGSIFFNGEDTTLLEPHHIVEKGISMVPEGRHVFSKLSTLENLEM
                     GAFTRHDRSAIQEDIERAFQLFPRLKERSKQSAGTLSGGEQQMLAIARALMAKPKLLL
                     LDEPSMGLAPILLESIFENVKEINAQGTTVLLVEQNTKLALSVADRGYVLQTGLVSLS
                     GTSKELRESEMVQKSYLGVA"
     gene            complement(52173..52949)
                     /locus_tag="PLL88_00665"
     CDS             complement(52173..52949)
                     /locus_tag="PLL88_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00101.1"
                     /translation="MNILSSSNITKRFGGLEAIHDLNVEIQQASISSVIGPNGAGKTT
                     FFNCITGYYKPEYGNIVFLDKEITGLPPHKIAEEGISRTYQNIRLYKELTALENILAG
                     EHIRLRSSFGDSLISTKRYRSEEKEAIDKAKKILNFVGLTGWGDQLACNLSYGAQRKL
                     EIARAIANNPKVLLLDEPTAGMNPAETQEMIRLIRRLRDELDITIILIEHDIKLVMQI
                     SEKIIVLDFGSKIAEGKPAEIQNDPAVIEAYLGKKNDLYQ"
     gene            complement(52946..53968)
                     /locus_tag="PLL88_00670"
     CDS             complement(52946..53968)
                     /locus_tag="PLL88_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00102.1"
                     /translation="MKNKEKTIAKLDQKVFRAILPALVSIIVLSIPLLSFPQKNLWIR
                     IIGYAGLYIILGLGLLVHVGLAGMFDLGFAAFYAIGAYTYAFLASPHFNIHLPFLLVI
                     LIAGALAAIVGALVCIPVLKLRGDYLGIVTLGFGEIVRMLLMNLTDITNGSQGIIQID
                     KPVIFGINFSNSTYFYYFIFLLIATLLFLLNRVINSRTGRAWLAMREDEDVAQMSGIN
                     TSKYKMLAFAIGAFIGGLGGAIFSSWQGSIFPDNFNVTVSINILCVIILGGLGNHLGV
                     ILGAMALIALPDILRGFSDYRMIIFGLLLIIMMILRPQGFLPRKAPQIEKNPCSSPEN
                     EKEVQL"
     gene            complement(53982..54992)
                     /locus_tag="PLL88_00675"
     CDS             complement(53982..54992)
                     /locus_tag="PLL88_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOJ00103.1"
                     /translation="MQKLIEQLINGMTVGSFYALIALGYTMVYGVLSMINFAHGDIFM
                     VGAYFGCFTITQLSKIGYTESNPILAIIFTFLAGAIGASLTGATVERLAYRPLRKASR
                     LATLISAIGVSFFLQEFVRLIPRFGSMVSSIHFGDTNLFPESWRIAIVNVTQIFGGAM
                     VKTYPGILGTGGIRIGEVVISYSRILIMTIAIAIMILLYLLVRFTKLGKAMRAVSEDK
                     DTAALMGINVNRIISNTFLIGSALAGIAGVMVGIFYLQIRPTMGFTPGIKAFTAAVVG
                     GIGNIPGAMIGGYLLGLAEVISIQFLPAVYKDTVAFAILIIMLIVKPTGILGGSGGKN
                     KL"
     gene            complement(55045..56271)
                     /locus_tag="PLL88_00680"
     CDS             complement(55045..56271)
                     /locus_tag="PLL88_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOJ00104.1"
                     /translation="MSKRFIFVSLLTVAMLLFAACGGTAPAEEAPAADAGESVAEDEW
                     GYIVVPAGEKVVLGLSSALSAGYAAYGQDMLNGATVAIQTFGDLNGWEVVVEGGDDQC
                     EGAPGVTVAEQFSANPYLVGVVGPMCSGTAIPAIDIYAENHVLMITPSSTAIAVTASG
                     NQNIFRVVANDDLQAEVTVDYLFNELGATTLGIVHDQSIYGQGIAEAVKSKFEAAGGT
                     VTGFEGITRGDQDFSAVVSIILEADPAAIYWGGMDAEGALLVNQLRTAGFEGIFFGPD
                     GIKSVPSFVEASGGAAEGAYMTFGAVGGATGYDEFLEKFSGQFGDPVAYGPGSYDAAT
                     IILNAAKAVATVDADGNLVIPKKALADAVRATPYDGITGHLEFDENGDLKVVSITVFQ
                     AQGNELVPVKVYDFGQ"
     gene            complement(56562..59810)
                     /locus_tag="PLL88_00685"
     CDS             complement(56562..59810)
                     /locus_tag="PLL88_00685"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ00105.1"
                     /translation="MAWFSPISQTKIIVPQRRKELLSRPRLLTLLDDLLDFKLIIIAA
                     PAGYGKTSLMIDYATSYDWPIAWLAMDPLDQNLTRFLTYFISAIQQRFPSFGVRSNAL
                     METLSEDQLDIDSIVTTITNDIFENITEHFILVLDDYHLVNQGKELDEFLSGFLQKVG
                     ENFHLVVTSRTLLTLPDLPLLVARSQVGGLSLEELVFQPDEIKKLFEKNHNVEISKEE
                     AEAYARQSEGWITGLLLTAQVGNKGIGDQAKISRTSGIGLYEYLAQQVLDQQSPDIQQ
                     FLLRSSLLEEFNPEMCQLIIGEALQVAGNWQSSMEDIVFNNLFVVRIGDEGFWLRYHH
                     LFRDFLQHRMETMYPEETRSILRKLAEYHTQSQDWEHAFVIYLKLGDIDTIIHLIESI
                     GSSFISKGKIKRLENWFSDIPEEKLRSNPKLMSIKASIEVNKGNLPVGLDLLNAVIEL
                     QQKKGDDKVSIAENLVRRSTTLRLLGKYQESHQDAQQALRLSEEIQKSNSIKAEAMRA
                     VGMTYYQEGILDEALTWMERSLALWQKSNSPEDCARIQAELGIIFETLGNFEEAEKAY
                     NQSLQFWKQIGDSIWQSNLYNNLGVLQHARGDFENSFMNLEKAMDYAKMSSNRRMEGY
                     SLASIGDLYRNLDAHQESLEAYSDAMKIAQDIEDQYLVLYLRLAETRIKILQKQFKQA
                     TTLLELAFNQAKRSGSQLEIEQCKVEKAILTYYFGDLSAALTMLLASLDFFKSMGQPD
                     TIFTIKLYLAVVLAKQKMTEESAAIFQEIIHNLNQPSSNTHILSTCFDLRNDLKLLSG
                     QAGIAEYIELILDQNANYQKKIQNIRRKIRRHASVVPFAEPKLVIRAFGKTEVYLNNK
                     LLSTSDWMTQTSRDLFFLFLSNPEGMTKEEVGLILWPDSSMEELKRRFKNAIYRMRRA
                     IGSNVVLFTDNYYHFNNAVDFEYDVLSFEQYIAQAENEKDAHKAIQLLESAVELYKGP
                     FLSDVDATWFGIERQHYFDIYMNALLKLYTCYMNQKDLEKALQINRQIIQNDPSNEEA
                     YCNAMSIYHALGNTAAIIRVFEQCKQQLAKEYGLEPSQNTIRHYQSFIQTKS"
     gene            60005..61075
                     /gene="wecB"
                     /locus_tag="PLL88_00690"
     CDS             60005..61075
                     /gene="wecB"
                     /locus_tag="PLL88_00690"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOJ00106.1"
                     /translation="MRIIHIFGARPQFIKVAMLARAWQGNCEQFFLHTGQHYDVDLSD
                     ALIADLDMPTPDLNLGVGSASPAIQTAQMMVGIEEFMIHKNPDCVFVYGDTNSTLAAA
                     LVASKMKTTLAHVEAGLRSFNRSMPEEINRIVTDHLSDYLFCPTRTALINLENEGIRK
                     EVALCGDVMADALAYYRQNTMDEEIVLNKYGLRKNDFILLTLHRPGNVDSKENLKEIM
                     AAVEEIGKMIVFPIHPRTKKMLQTFRIKLPTNVKAIAPLEYTEMIILEQAAQCVMTDS
                     GGIQKEAYLMGTPCITLREETEWVETVAAGWNVLVGASRERILSAFNDFHPTGEQPAL
                     FGDFHAAEKIVEQTLEWMRGMD"
     gene            61079..61681
                     /locus_tag="PLL88_00695"
     CDS             61079..61681
                     /locus_tag="PLL88_00695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ00107.1"
                     /translation="MDYFVHTSALVEDGAHVGEGTKIWHNCQVRSGAHIGKNCILGKG
                     VFIDSGVVIGDAVKIQNYVSIYHGVTVGNGVFIGPHVCFTNDLFPRAINKDGSPKSME
                     DWIVSETHIADGASLGANATIRCGIRIGEWALVGAGSVVTRDVPPQALVFGNPARFQG
                     YVCKCGVKLDVMKTGETSTDYVCPNCYEEYNFMNKQEGIE"
     gene            61678..62769
                     /locus_tag="PLL88_00700"
     CDS             61678..62769
                     /locus_tag="PLL88_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOJ00108.1"
                     /translation="MNIPIAKPILGDEEKQAVLCVMDSGMLAQGSRVEAFEEAFADYC
                     GVKYAIAVSSGTTALHIAMLAHEIGKDDEVITTPFSFIATANCLLYVGAKPVFVDIDP
                     ATYNIDPALVERAITPRTRAILPVHLYGQCSNMDAIMDIAEKHHLIVIEDACQSHGAA
                     YKGKKAGSFGTGTFSFYPTKNMTCGEGGMITTSDAVINDRCRVIRNHGMRRRYYHDEL
                     GYNFRMTDIGAAIGLEQLNKLERFNAIRKENASFFSQNLRGVVVPVEVKECSHVYHQY
                     TIRIKNGKRDALREYLKERGIGTEVYYPVPIHKQAFIQKIFPALPVLAQSELAAEEVV
                     SIPVHPSLSEIDRKAIVESINSFMEERGD"
     gene            62762..63757
                     /locus_tag="PLL88_00705"
     CDS             62762..63757
                     /locus_tag="PLL88_00705"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ00109.1"
                     /translation="MIKTAVIGAGSMGANHARIYAGMNAAKLVATADLDKDRCASIAE
                     KYNVSTYTDYRELLEREKPDLVSIVVPTASHMAVAMDCLQAGVHVLIEKPITSTLDEA
                     GAVIERARSEKLKLMIGHIVRFNPAVQELKKHLDAGELGRIFQIVCHREGPFPTRIRD
                     VGVVVDLAPHDVDIMRYLSQSEPIRVYAETERRIHTEHEDLLLATMRFKNGITSALNI
                     NWLTPTKIREINVLGEKGMFQINDLTQDLYFYENSLAVNGIWDSLKTIQGVNPGKMVR
                     FAFDRKEPLEMELTAFVEAVQNDLPVPVTGEDGYEALRIASALVQSGLTHQVIEL"
     gene            63759..65075
                     /locus_tag="PLL88_00710"
     CDS             63759..65075
                     /locus_tag="PLL88_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOJ00110.1"
                     /translation="MSLQELSEKISGKTATIAVIGLGYVGLPLAACLADVGFMVLGVD
                     RKQERVAVINQGKNPIGGKEPGLDELLKKTVSAHYLQATTDYQQLRNCDVVFINVETP
                     VEEDHKPSYQALRSVLKALGPVLKKGALVIVESTISPGTMKNIVLPDLEKSADGKLGH
                     DFYLGNCPERVMPGRLLANIHSMQRVIGGADEQTAQTMALIYKQFVNADLDLTDWVTA
                     ELVKTVENAYRDVQIAFANEIAILCADLGADVWKVRELVNKTPYRNVHKPGAGVGGHC
                     IPKDPWLLASSLSESKELSLIPAARAINDSMPEHVFTLLEKGLSTLGKMLKDSRILIL
                     GYAYLENSDDDRNSPSELFARRLMDGGCSFSIHDPFIERYQGDIYHMAKDCDALVLMT
                     AHDQYRDLDLKIMRQVMRTPLIIDGRNAFADLTDLHGFEIICLGKG"
     gene            65080..65934
                     /locus_tag="PLL88_00715"
     CDS             65080..65934
                     /locus_tag="PLL88_00715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ00111.1"
                     /translation="MIWALIPAFNEETALLSLLEKIKQGLEGYEYKIVVVDDGSFDKT
                     PLLLAKAAQKYPLEVITHPINRGLGETERDGFEYIAAHSNHDDIIVRVEGDNTHEPVY
                     IVNLVSRLKEGYDVVNTSRFQPGGGQVGLNFYRSFISRCANYFMQVMFNIKGIRDYSC
                     GFRAYRAKVIQDAVAIFGNNFIQLKGVGFTSTLEILVKLKMLGCTFAEVPFVLRYDQK
                     QGKSKMVGSVTTVGYLLLALFYNWPFGGWKHSYRGLAVLYQEDVQKAAERYGINKSSG
                     RFLTKINS"
     gene            65944..67833
                     /gene="asnB"
                     /locus_tag="PLL88_00720"
     CDS             65944..67833
                     /gene="asnB"
                     /locus_tag="PLL88_00720"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOJ00112.1"
                     /translation="MCGICGILHFDAQRTVDATVLTAMSEKMIHRGPDDFGLYVAGSL
                     GLAHRRLSILDLTAAGHQPMSSTDGRFWIVFNGEIYNFVELKRYLVAKGYSFRSRTDT
                     EVLLNLFIEEGKESLHKLNGMFAFAVWDSKDKKLFVARDRLGVKPLYWYRDEHTLLFA
                     SEMKALFQFPSLKAMLNTDGLQDYLSFQFCLGEKTMFKSVHKVPPGWWMEIAQNGDTK
                     SGCYWDLEFKVDPDHSEGYFLQTLGDLLQDSIALRLRSDVPLGSHLSGGLDSSVVASL
                     AASNLQQPIHTFSGGFKEDAIFDETKYARIVAEQKNTIHHEVFPTAQDFIDFLPSIIY
                     HMDEPAAGPGIFPQLMVSQIAREHVKVVLGGQGGDELFAGYARYLIAYLEECIRGGIE
                     ATQEDPGYIVNFQTILPNLPQLQGYQALMRHFWKDGLFEPQEARYFRLIDRGDSLRTF
                     FHTDLIGQNHYSSYEEYRQIFTTNAPASYINRMTRFDIKTLLPALLQVEDRTSMMVSI
                     ESRVPLLDYRIVELSACVPPRFKYAGGQSKHILREASKPFVPHEILQRKDKMGFPVPL
                     DIWYRKDPVRAFVRETLLSSASMKRGWFDAQAVRETLDQELPFGRTIWGLLCIELWAR
                     AFLDA"
     gene            complement(67863..68888)
                     /locus_tag="PLL88_00725"
     CDS             complement(67863..68888)
                     /locus_tag="PLL88_00725"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HOJ00113.1"
                     /translation="MITNRTSEELLSQKKMKQQTLNDTFVAEHRAYKIVIVGSGKVGT
                     TFAYTLLLHGLVGEIVLIDVDKERTEGEVMDLNHAIPLANPIRIYQGDYKDCRGADVV
                     VITAGSAQRSGETRLDLLKRNIAIFNQIVPQVIQYTRDAILLVATNPVDILSYATHKI
                     SGLPAEHVIGSGTVLDTARFRWLLGRHFLLDARNIHAFIIGEHGDSEVAVWSSANIAG
                     MPLDAYCRFIGCEIPISQREEFTSQVRNAAYEIIQRKGATFYAVAAGLARIVESILRN
                     QNSVLSVSNLVPGYYGIRGVYLSLPAVVGEQGVEHVLELQLSPSEEQALVASAEVLKK
                     QIDQLEI"
     gene            69443..72013
                     /locus_tag="PLL88_00730"
     CDS             69443..72013
                     /locus_tag="PLL88_00730"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HOJ00114.1"
                     /translation="MIYHFLPVDLLFFNAAIISLFVAVIILIKRVEPGGTAFAAVMLS
                     LALWLIFRVCEGVSEEIPQKIFWAKFEYLGIATLPVCYFIFVSQFSRKDRWITKGNLI
                     ILSLLPVLTLVLVFTNEYHGLIWSDIYPASTPQVESLIYEHGFFFWVYWVYSYVLLFF
                     GIYRLIATLLNFSKEYRLQMIVLILATLLPWLGNLLYIARLSPIDGMDLTPVGLAFSG
                     LVIALSLYKGQIFEVAPVARNVIFDAMQEGILVLDIKGNIVDGNDATAKILDIPLKNL
                     LKKHFTESLSRYPLLIENLQKIGNNKFEMCLDENKQRFVQVSISLIVTNINPTGQLLV
                     LQNISERKKLELYESEQRKFAEALAGIAATLNSSLEFEVVLEKMLAIIHQVVPHDAAN
                     IALLDDDKKMRFVKLIGYEKFESKKVIESLDYSINDIPDFKMMAEEKEAVVITDTHNS
                     PAWISNPKVAWIRSYVGSPIVAGGKTIGFINVDSSVPNLYTPEHAQRLKVFADEAAIA
                     IQNARIVEELKERNQDLGILYEVGLAMTSGLDNQEIINGLIKQIKNFKNIDIFFLMLF
                     GEDSMKNMLYWYTLNDKTIKTIELTKPWSGNFIHQILEQKKTVYLPNQERQDIITGIP
                     DEKQLSFSAMRSYLGVPLMQGKDVIGILSVISKQSGAFDQKQIRLLETITSQVSITLQ
                     NVKMYERMKELAIVDELTGVYNRRYFYIAANKEIERALRYKKALSMILIDIDHYKDVN
                     DHYGHMAGDKVLQKITQAIQKELRGSDIFSRYGGEEFLILLSDTKGEEAAVVAERIRT
                     TVEALHVQYNEEEISVTVSLGVTRLTAERNSLQEIIADVDQALYGAKQKGRNRVEYIA
                     "
     gene            72135..73031
                     /locus_tag="PLL88_00735"
     CDS             72135..73031
                     /locus_tag="PLL88_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOJ00115.1"
                     /translation="MMVTYQYTSEIELRKALLDEIVRAAGLPLNNFWRKFVEMIFWLP
                     INRFSKLGSYFDQMVYQHGFSKAVDISLKLLINDIVVLGEVKAPATGPLLVVSNHPGT
                     YDALCLAAKLPRDDVKIVSSNISFLKNMKNVNSHFLFASIDSYVRMLVIRHAIRHLEK
                     GGALIIFPSAHMDPEPSCMPGAMEALDEWSRSIEIFIQKVPSTVVQVAVVSRVLTSRY
                     LSHPLTRLRKGRRERQRIAEFMQIIHQMIYRRKFNQTPLVSFAEPFKSSDIGPDDKID
                     RVLPYAINQAKQLMVAHLPENY"
     gene            73150..73866
                     /locus_tag="PLL88_00740"
     CDS             73150..73866
                     /locus_tag="PLL88_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00116.1"
                     /translation="MKRSAVFLSFHVLSKFLLLMLASSCSILPFGEAWFQDDKTPGTQ
                     PTGIIFFDDFSSNTSGWDRVETDLGGTDYVSGGYHIVINEKNTDYFSTLYRTYTDIGL
                     QADAQWVEGPYDNNFGLICRFQDEKNFYAGMISSDGYYGIFKIENGDYTVLGHETMLQ
                     SETIGITDSRKQIAFRCYQDFLFLYVNGILLDVQQDKTFNAGDVGMIAGSFEDAGVHI
                     LFDDFYLIDVTQTANQEMQE"
     gene            73863..74855
                     /locus_tag="PLL88_00745"
     CDS             73863..74855
                     /locus_tag="PLL88_00745"
                     /inference="COORDINATES: protein motif:HMM:NF027787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOJ00117.1"
                     /translation="MKKYPFLFLFFLMILSGCQNFLDGEENASTLVPAVTETATPTVW
                     ATLAPFSATSTPIVLATLNPVELQGEITFQSDRSGNMDIYVYDLRSESLSRITSGESA
                     DVFPSWSSDGEQIVFVSDRDGNPDIYIVNRNGSGLKRLTDDPHDDVFPTISPDGKTIA
                     FVSNRGGNDEIYTMDINGENLTQLTNNVFADGFPAWSPDGEQIIYQSDRDVNTELYIM
                     SKDGLDLMRLTDSTAEDAQPAWSPDGKRIAFVSTRTEFEEIYIYHLDTHAVEQLTNFQ
                     AQTEVPKWSADGKYLIFASNYEGNRDIYIMAVDSRAMLKITTSTGEDFFPDWNP"
     gene            74958..75182
                     /locus_tag="PLL88_00750"
     CDS             74958..75182
                     /locus_tag="PLL88_00750"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOJ00118.1"
                     /translation="MKRLYRKRNDKMLAGVCAGLADYLDIDPTIVRIIFLILVFAGLG
                     GVLVYAILWVITPLEPSASEDTIDARSKGE"
     gene            complement(75267..76916)
                     /locus_tag="PLL88_00755"
     CDS             complement(75267..76916)
                     /locus_tag="PLL88_00755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ00119.1"
                     /translation="MRNKANAAKRIHAGRNARLGFLTGLLFLIFVYAYEFLRTGTRFS
                     LNSIQQIHDSNFFFWIIDFIPFAIFFVTYFSTRTGSGTRAHALFKSIESQKKSEILKQ
                     KIFYESLINNNPAAIVTMDADQKIISINPAFSRIFQYTQDEVYLKDLDTILADDPDLG
                     QAQEFTKTVLQGGTIYGTGKRRRKDGRLVDVKIYGVPIFLGEKRIGVLGMYLDITSRI
                     RNEMAIKENELRYRSLFHDSPISMWEMDFSALKEWIENLEFNSQDKLNLFLEKVPDLE
                     KEFSSLIKVINGNQATLLLFKAADLDDLQKNFNGIFIEQSAESIRSIIMALYNGSCQV
                     ETEMPCRSLDGLIIHTIVRLSLINGFEKSWSRIHLSILDITERKWAEERMRFLNMHDS
                     MTGLYNRAYFEAEFKRISQGRQFPLSIIVCDLDNLKKINDQYGHAAGDTIICKAATIL
                     QESFRGDDIVARLGGDEFAVIISSLEESAVLKIVARLRNNIQKYNQTLPADDQAHRMN
                     FSIGAATTHCESEVEALFKLADHRMYCEKLGKRVSTANQHG"
     gene            77227..77949
                     /locus_tag="PLL88_00760"
     CDS             77227..77949
                     /locus_tag="PLL88_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ00120.1"
                     /translation="MTNEAPLREQQHVIEAFAELAPKYEKKMDSELNLFWGWSYRELI
                     QTLLEDYSLVHAKRILDLATGKLIIPRAIAAINPTVKQIVGLDITFRMLQLGKTILSR
                     NSRIRLLCASALEIPVRPASFSLITCTLATHHMDGRRLLSEIHHILDWDGQVVIVDVG
                     ASKTWMNPIVRGVMRVLAFLYFMVRENVARAWAEAEALSNIHTAEQWEDELKKAGADL
                     AHLKPPHMQPKDNIIERLIKVD"
     gene            77961..>79977
                     /locus_tag="PLL88_00765"
     CDS             77961..>79977
                     /locus_tag="PLL88_00765"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ00121.1"
                     /translation="MIWNFEFIVILLAFISYLALYLVVWLTKPKTKEKEAFRFYLLMM
                     ALWKLSAIMVVGQLGDIYFWFRMMTVSSVMSLIGSHRFVQSILPQRTSWDRWIYAYIA
                     LAVSVTLFTNWVVSYAYMNGTELVYSFTPLVGLVAGPGYALMLYNTYRLIKEYSQARS
                     PQIRNRLKYLIFGFIFIIIGSIFNFTDLGQYPIDIFANLITAILISVAMLRHQLLDIN
                     VIIRKSVLYAIPTSLIGALYFLVISSVVRAFQLSVSQQVLVSMVVAIAAALIFQPFQK
                     LLQDWINRIFFRERYDSNLMLQRVIQTASQEIDLQRLAGSIINEIISTMHIKRARLFV
                     SHEDGGPFILISGSGLNYSRQKEFRVDHPLVLKMGKTQEFLMWGELESSPLLKSLWKD
                     ELDYLVDLRAELFIPLRTKNQLIGILVLGEKMSETPYSQEDLNILTTLASQTSLALQN
                     AKLYSLAQKELVERKKAEKGLQLQLKRLSALQDINVAITENFDLQVPLVMLLDQVVSE
                     LKVDAADVLLYNPENKSLNFIAGRGFNTDALRFTSLQLGQGMAGMAAQKHKPVYVKDL
                     SAERTTLKQSPLLEGENFVSYYGFPLVARNQIKGVLEIFHRSVLEPDQQWLDYLNTLI
                     SETAIALDNARMFNDLEKTNMELVSAYEATLEGWARTLEMRDRETEGH"
BASE COUNT        21915 a        17175 c        19521 g        21366 t
ORIGIN      
        1 tggcgctggg atagatggat ctctctctgg aaatgaagga tttccggagt ggctgcgcca
       61 atcggctaat aatttttggg gttggccaat cccgaatgca gagaaaacgt atcccgtaca
      121 ggatgatgcc gatttgatta tcttggtggt caggcaatct caggaaccgg ttacgctctt
      181 tttcagcggt ccctttacca acttggcttt ggctctgcga aaagccccgg aaattcgcaa
      241 caacattgct gcgctgtata tgatgggtgg tgcagtttat gtaccgggca atgtccacga
      301 tttctatcct gattctgcta acgtatatgc agattggaat ccgtattctg atccgcaggc
      361 gaccaaagaa gtttttgaat ctggcttgca gatgtatctc atcccgctgg atgctaccaa
      421 ccaggtccgc atcgataaaa gcgatacgtt ccgatggcgg gaagggggag agattgccaa
      481 ctttgctgct gatatctatg acggcttgat ggattctacg ggaaaagaag aattttatat
      541 ttgggatttg atggcttctg aggttatgct gaaccatgca ctgtgcgatt ttacgccgct
      601 gcatttggat gtgatcacag aagagtcgga tcattttggg cagactgtgg ttgttccaaa
      661 tgaagaacca aatattaatg tttgcctaaa accaaaggtt gatttgatta aacaggggtt
      721 gattgatgcc ttttcaaaca gccggtgata tccccgcctt atccagggga aaccatcaat
      781 agaactatcg aataaatcgc ttgggtgcta ccctacggcg atggtcaagc tggctgaaag
      841 gcctcgccgt caccacccag ttcaccgatt tggataatcg tgaggtgaag ttgtagatac
      901 tgtcttaaat gtcaagagtt agcaatacaa aaggcaagat tgaaaggatc tctccattta
      961 catattaaac cagtcaaccc gctttttccc attttctccc cgcgatgtca ttccgagtga
     1021 gcagcgcgat cgaggaatct ctccatttct attcttcttc cagtcgggta gggtgggttc
     1081 ctaacccacc actgctcctt gtggaaaaac aggatgtgaa gtgttagcct gccagtaagt
     1141 cggggtgcgt tcccaaccca tccttgattg cacaacgttc cggctggttt agaagatgta
     1201 ccttgcacga aagagaatag ctatagggtt ggcattgtct gtacccgaaa aacgtttcat
     1261 tgataatttg acgagttgtg aacagaacca aacggaaaga aaaattcgga caaaaaagag
     1321 ttacaatcag aataaaagaa gcaagattaa gcttttaagc aggaaggaga atacctccca
     1381 tgccttttaa tcaaaattct cgtctcgacc catcccaggt ccaggatcgc cgtggccgcc
     1441 gagttggacg aactgtggca atcggcggcg gtggcgtagg actgatcata gttgtggtcg
     1501 cattgctact gggagcggat cctggcgacc tgttaggaat ggtcgactcc aatttgcaat
     1561 tatccgatca aaatataagc gagatcagtg atctacaggc ggagtgtcag accggcgcag
     1621 acgccaatac cagggaagac tgccgtatcg tgggttacgt caacagcatt caagaatact
     1681 ggtcttccga atttgccaac tacggcgtac agtacaccca ggcccagaca gtgcttttca
     1741 gcggattgac cgagggcgct tgcggctatg cttcggcggc gactggcccg ttttattgtc
     1801 cggacgacca gatggtctat gtggatctct ctttctttga tgatctgacg acacgttttg
     1861 gcgcgcaggg aggtccattc gcagaagcgt acgtgctggc tcacgaatat gggcatcaca
     1921 tacaaaacct tctgggtgcg ctcgaccgaa acagtgcgag ttccacgggc ccgcgaagca
     1981 tgtcggtttt tactgagctt caggcagatt gcctggccgg cgtgtgggca tatcacgctg
     2041 ctgaaaccgg ctacctgacg gtccccacaa gcgaggagat tgcccaaagt ttggatgctg
     2101 cctcggctgt gggtgacgac cgtatccagc gcgagacgca gggatatgtt tcacccgagt
     2161 cgtggacgca cggatcttcc gagcagcgcc aggcagcgtt tgtagacggc ttgcaatccg
     2221 gggatatcaa tacgtgcaat acgcccgggt ggacggaata agccggtttc catctcaaca
     2281 cttggcggaa tggaatcagg gcgatttctg ttactgaaac ttatactgct ctgatcgctt
     2341 cctgaataaa aaagatcgac gatcatgaga tggaaggtgc atcccgttta ttcgcatcat
     2401 tctgcgaaaa aaagaacaag cgctctccga gtggagggcg tttttgttgg tttttttctc
     2461 aatgagttct cgtgggcaat gctataattt tgctatgaat gagatctatt cggatacgca
     2521 tccgaaaatc gccgcgcttc agcgaaaaat gctgcgccag gcaagtcccg ctcgaaaatt
     2581 agccatgctt ggggagatga acaaaactgt caaagtactg gcattgtccg ggctgcgctc
     2641 ccgttacccg gacgaaaccc ctgagatgca taaacgtcgg cttgccgacc tgatactcgg
     2701 accctccctg gccggtttgg tatacggtcc gttgattgat aatcgctaaa tgctttctga
     2761 acccattcaa gtcaccctga aagtagtcac aacctttgag cttctctcta ttccatattt
     2821 tattggcggc tcattagcct cggctatcta tgagactgtt cgcaatgagc aaattcccaa
     2881 ccccataatt tcagggggaa gggggagaaa tgtcctcgat ccaaactgat atggattcat
     2941 tccagtccca actcgtcaaa ggctccatcc aaagagccta ccgtgctctc ctcgcttaca
     3001 tgctcgcgct gcgcagccaa tttgcgtcca aatacgggga gcctgccgtc tctgctctct
     3061 accagggata catggacatg acttatttcg ctctctttcc accatctctc aaatgtcttg
     3121 acctaaaaat agccattgtc ttcaactatg acgctttcag gttcgaagct tggcttgcag
     3181 cacgcaatcg aaaagttcag cgccagtatt gggaactctt caaaaataac gattgggcag
     3241 agtatcgtgt cgtcgcgccc gcagcgggca ttgattccat tctcgagtgt gacctggcca
     3301 aagatttcga ccttagcaag ccagacgctt taacttccag cattgaaatg actacagttg
     3361 cattcatcga caaaattgag agatttctct ctgaacaaca gccccatgaa ggagctacct
     3421 cttgtagttg agcaacgcct gatcaggata gccgaacaca gcgcacacct gactgcggga
     3481 atcgccgtac gctgataaca tcataaatgt aagcttggct gcactttggc aaagagctta
     3541 ctggtgatag ccagacgtat ggcttatcgg agtgttacct acacaaccag gtcaaaattc
     3601 ggtaaattaa gtatacagat ataagttcag accagtgtgt cagaaatatc atgctcctgg
     3661 caatcaaaga gaaggtgatt cattctgatt tctatggctg atttctatgg caatagaatt
     3721 accccacgtc tagaattatt atctataatt aatttcagga gaatcattcg cttgccagaa
     3781 tcacgagaat ctaccattaa aggcagcaac ttgaagatat ttggacttta ctaacgagtt
     3841 ggaatcatca acaggaccag gattttaaac aacgctacag ccaattcact ggcaggcaat
     3901 aacaggtcgt ataaataaaa atcgcaagaa ggtcctcatg aaaattaaaa aaacttcccc
     3961 caatccagtt ctgttaatca ttttattaat ctttctgctt ttcggttgta cgacagccat
     4021 acgaagcgaa accgctgcgg ttgaagaaaa aacgccgact cccattacct caaatcagaa
     4081 actacgaatt cgtctcttga caacctccga ttggacgatt ttcaatctga tatcaggagc
     4141 gacctgggta acaaaagaac ttatttcaac aagtcctgaa gcaactaccg cgaaaataga
     4201 acaaaatcaa ttcacattag atcaacccat cgatagtgca gaagcgggta agggtgtgga
     4261 gatgaccgta gaattacttt tatctcccct ggcgactgaa aatgaactgg ttttcgaaat
     4321 tgaacgtggt gatataggat acacccaggt tgaatttttc gggttcagcg gaacaaattg
     4381 ggaagtgatg aaaaccgttc aatgggctgg gaaaagcggg gatggggtaa atctgtatag
     4441 cgtcgagatc ccctttgccg aacttatcga aattgctcct acaactatcg tggattctca
     4501 acaagaagat acttctaaaa tcgataattc aaaaaatatt cagggaacag aggaataccc
     4561 ctggtggaac gacagcgtct tttatgaaat atttgtgcgc tccttctacg atagtgatgg
     4621 ggatggaatc ggcgatttta atggcatagt agaaaaaatg gactacttaa atgatggaaa
     4681 tcccaacata tccactgacc tggggataac gggtatttgg ttgatgccga ttaatccctc
     4741 tccttcttat catggttact ctgtaaccga ttattattcc gtcaatccag agtacgggac
     4801 aatggacgat tttaaaaact ttcttaacga agcgcataag cggggcatca aagtcatcat
     4861 tgatcttgta ctcaaccaca cctcaaacca acatccctgg tttctcaatg cggcagatcc
     4921 aacttcacct taccatgatt ggtacatctg gtctgatacc gatccggggt atgttgggtc
     4981 atgggggcaa aaaatatggt ttgaacaggg tgatgaatat tattattcca ctttttctga
     5041 attcatgcct gacctaaact acacaaaccc agaagtcacg caagaaatgc agaatattgt
     5101 tcaattctgg gtgaatgaag tgggggtcga tggttttcga ttggatgcag caaaacactt
     5161 gatagaggaa ggaatccaac aagcgaatac gatatcaact cactcctggt atgaaaaatt
     5221 tcgacctgct cttaaacaga ttaatcccca tgctttgacg gtgggcgaga tatgggaaga
     5281 tactcgcatc aacgctgaat acttacaggg tgatgaagga gatccggtca cggattatcg
     5341 attgtcgctg gaagcgagta atttaccagg aggaaactac gttcctgcac caatcttggg
     5401 aggcggtgat tttatgccgc ttgagataaa ctcgaatggt ggtttttcgc gctatacgcc
     5461 aattcttgaa gtgccaccat atgcgataat tacccttggg ttttaaatgg atggtttttt
     5521 gagctaagat gtttcctaaa gatttctgat ctgaaaattc atcgttattg gtgaattttt
     5581 agcaacttca acatctctga aataaaaata agtggataag cttactttta agtttcgatt
     5641 gtcttgtttg gtggactaat atagtgaagg acccctacat attcttgaat ccatatttcc
     5701 aaacaccaaa gaggtttcgt ctcttcagaa taatgtactt gggtgttttt aataatggga
     5761 gttccttttt ttattgctag ttttttgcta atgaactcgt cacttattag tggtttatac
     5821 gaggttgaga aataacaaat ctttgggtca atatttcttt cgataaaagt gaatagcgat
     5881 tctccaatta tttttcttga tatcttttga ggcagatatt tcgatgggat gaggtttata
     5941 tcataactaa aaggaacccc atcgcttgtt ctcagtcgcg aaatttttat tacaggagaa
     6001 tttaattcaa cacttaattt gttcgatagg tctgtatctg cattaattct ttctaaatta
     6061 aactcacttg ttccaggaat ttttccacaa gatttaatat agtcggttat tgaaaaagaa
     6121 tattcaattc ttacgggcat tagtgttttt gcattccctg tgatgaatgt ccccacacca
     6181 tgcctccttt ttataaaacc ttcaacctct aacctggata gagcaattcg caacgaagaa
     6241 cgactaatgt ttagtttttc agttagttct tgctctgacg gtaatcgcgt gcctattttc
     6301 agtgagttgt tatttattaa tagttttaaa tactcataag ccctttgagg gacattttcg
     6361 attgaatcct gtaattttga aaactcaaaa tcgctcatga caatattttc taatgtctag
     6421 tattagactt ttatccttga caaatttttt attaagtata caatattttc aaagttatgt
     6481 tgtctgacgt ctaatgtcat acgacataag tgtaattata aaggcagaat attttaattc
     6541 ttattgagaa ggataagttc aaagatttct ttgaaagaag agaccattta tcctgatgaa
     6601 ctaaaaaaat ctttaaatta tcgaatttta ctcaaggtga gttgtatctt tacaaaaaca
     6661 gaaaggagag ataaggtatc ggtcgataat tttgaaatgt ttttaatagt gtcttattta
     6721 ttctcttaga aaaacgaaag gaagaacgat gtcttacgaa aagttagcta ctgaactggt
     6781 agaggctcta ggcggtgctg agaatatctc tacaattaca aattgtgcaa caagattgag
     6841 atttgcactc aacgacaata ccaagtatga cgaagaaaaa gctaagaata tcaagggtgt
     6901 cataggggtt tcgagatttg gcaaacaaat tcaagtcgta atcggtccgc aagttaacga
     6961 agtatataaa gagattgcag ctttagacag tgtcaaaaaa gcattgagct cttcgcgaat
     7021 aaaggttaaa aaagcatgga tggacattat ctttggttac attacaaatt gtataattcc
     7081 tatttatcct gcgttcatcg caggtggctt gctaaaagga attcttgttc ttgccacaaa
     7141 cctgaagtgg atctcaggta cttctgctat atattctgtt ctttatgcag ccagtgatgc
     7201 tgtcttctat ttcctgccag ttttgattgc ttactcggca gcaaaatact tgaaatctaa
     7261 tcaagtaatt gcaatgattg ttgctttatg tttcgttatg cccaaataca ctggcatttt
     7321 agggacagat attatcaata aagctttttt ggttagttac tcaagtcaat tatttcccat
     7381 gcttgtagcg attcctcttg caagctttgt tgagaaaaag ttgactgaag ttatccccaa
     7441 cactcttaaa tccatcattg tgccaaccgt tacagtgatt gttacagtgt tagctgtatt
     7501 tttctgtatc ggcccgatta cttcctttgt tggaaatgtg atttataagg cagtaaatgc
     7561 aatttatgca tttagtcccc tcctttgtgg aacttttgtc ggtggggtga gtggttatct
     7621 aacaatcatt ggtgctcatc tggcgttgat tccaatcatt atattgaata ttacggaaac
     7681 ggcatcggat tggctcctcc caatgatgca gatgacttgt atggcacaaa ttgcaattgc
     7741 tttcgcgtat tttgcagcaa ttaaagatcg cacgagtgaa aaaaaggcgg ctgctcttac
     7801 tgcaggattg actggcttac tctcaaatat tacggaaccg gcactctttg gttttgtagt
     7861 ggaaaataag atgatgttga tttacatggg tattgctggt gctattggcg gtgcgttggt
     7921 tggtgcgaca ggtatagttt atagcacgat aatgtcgggc attatgactt ggattgcggc
     7981 ttccccaact ttaaccatga atttactgtt cgatgctttt aggttgttgg atatggctat
     8041 tgcttttgtt ttagcgttta catatttaaa gaagaaaggc gttgaaggat aaatacctac
     8101 aaaggagaaa attgtgtaaa tcacaatttt ctccttttgt acttctgaac attattgatg
     8161 gacatagaac tttaacaatc tcgtaaatga aaacaaaaaa ttcttcttta caagccgatc
     8221 aaagtcttgg actaattcca gagttttaga ttttactatt tatccgtaag tagcaattta
     8281 caagaaaaat tgtgaaaaat catatagtca tgtttcccat gatttcgcgt gattgctcac
     8341 atacctctag cggtttagtt tcataaatca atctaagttt ttatgaaatt cacctttttg
     8401 gtgtatgtgc agacatatga gaaaaaatga ctgaaataaa gttaagggag caacatttta
     8461 aagtgtatta agtcgatttc agcttatacg gttgattttt ttaatagcct tctgatgatt
     8521 cactggtgtg cttataaaag gagttttcat gaataatcga ttgatgataa aagaaacatt
     8581 tttgaagttt gtcagcataa tggaaagagg cactgttgat gaattgcgtg aaatccttac
     8641 cgaagatgtc attctaatga cttcgaacta tggaaatgct aaaggaattg agaacgttat
     8701 aaaaaaacta agatggaatg gaatgcccgt agatttttct cgttcacgta tctttaattt
     8761 tgttgtacag aacgacgaaa atatagcaaa acaaagcgct gtggtaacaa cattagtcgg
     8821 taataatgat cctgattatt ttcactattt tcatttcgcc gggtattacc tgaatgatct
     8881 tgttaagatc aatgatcagt ggaaaatttc ttatatcagg ttcaaccttg acatggaaga
     8941 tgggaacaca ttatttgtta ggggttggtg gcgtttgatc gattaccggt attttgaagg
     9001 aacagcatat tatcctattg tgagtgaaat agatgctccc tggagagcga taaggaaccc
     9061 agagcccatc ttagatgaaa aagaacaaat cctggacacc tttaatcggt tgaattgggg
     9121 aatagatcat gcagatttcg aactagccat ctcaagttat gacactgaaa atggtgattt
     9181 tagtggaatg gctaatgtta tgaaagaaca gaatctgatc ccggagtggg acgaggcggg
     9241 aattcgcgat gttgttcgat tattgaaata caaaagatat aaagaagcga ttatggagca
     9301 tatatggaaa atccataaac tcaacattga tggtactaga gcagaaatga cagtgtatag
     9361 atatgagcca catagattgg gaacgacaaa attcaataag ttgaatatgc attacgactt
     9421 ttttactact gttttggact atgagtttgt gaaaagagat ggcgattgga gaaatggtgg
     9481 cggttggaag attacaaaag aggtaccgag acgtggtcgt attatttctg aagtgactta
     9541 tgataaagaa aagttttacg aataatagaa atgaaatata tgatttgtta ttcgaagaag
     9601 attcgcaaat cgtaatatat tcttaccaaa aagaagacct gaatgatgat cagtcaacca
     9661 atttagaaaa cttgtttctt ggcataatct ctgaaattga ttgtttgata agtgaattga
     9721 taaaagaacc acagttagct gttctgcttt ttaaacgaaa taatgttgat tgcttactta
     9781 ttgcagatcg aaattctgcc ctattgcaaa tggcaattta taaatatgaa agtatcgatg
     9841 atatttgttg tgaaaatcta tttgtatctg aaatctgttt agagaaacat gagccaagcg
     9901 aaggaaaaaa agcactatat ttttttgtct atttcttttt tggagatttt aaccatatgg
     9961 tttcgttatt ggatgaccat gtcgagattt taaaacatgg taaacgctat agggggaaat
    10021 gctcagcgat aatgatattg aagtctttaa gattttcaaa gtctatcaga gagagcgatt
    10081 gtttcaagat taatagcact tttgatgatt atatttttga atttggcagt caaataataa
    10141 gaattccaaa tactgatgac aatataaagg aaatttttct cgaagaaggt tgaattttga
    10201 gttgcacaaa gcaattaaag gagttttgag tatgataaat atttttgtcc cttgcacggg
    10261 agacatagtt gacgtaacta caattaatga tgaggtattt aaaaataaga tgttggggga
    10321 tggtatggct gtaattccaa atggaagcct ggtatttgca ccgattgatg gaaaaatcgt
    10381 ttcaatattt ccatcaaatc atcaatttac catgaaaaca gacgatggct tggaattact
    10441 gattcatatt ggtattgata cctatgatgt aaaaaagaga ttccttgtac gtttagtaga
    10501 ggaaaatcag tatgttatga gaggaacacc agtgataaaa gcaaaattta ataagctaag
    10561 agcaaaaggc tacgatactt cggtaatcgt cgtcgtgtta aatcacccaa tattcgacaa
    10621 gacaaaaatg tcgaaaagtg aaagcataga tacggtattg ttcagtatat gatcaacccc
    10681 ccaaaattaa aggagtatga atggtgaaaa aactaaaaat tgcaactatt ggcggtggga
    10741 gtagttatac accagaactc attgaagggt tcattaagag aaaggacaag atcagtataa
    10801 gcgagttgtg gttagtggat attgaagaag ggcgtgaaaa acttgaaatt gttagcggtc
    10861 ttgccgagag gatggtgaag gcgaataatc tggattggaa aatctatgca acacttgata
    10921 ggagggaagc gttaaagaat gcagattatg ttacgacaca gtatagggta ggactacttg
    10981 atgcaagaat taaagatgaa cgcattccaa taagttataa catgattggt caagagacga
    11041 acggggcagg cggtattttc aatgctttaa gggcaattcc ggtcatcaaa gatgtaattg
    11101 atgatatgaa agacttatgc ccggatgcat ggttgattaa ttttgctaat ccctctggaa
    11161 tggtgaccga agctgccata aattattgtg gttgggaaag agctataggc ttatgtaatg
    11221 tcccaatatt tatggaaagg aaagagtcag aagcactagg tctaagtaat gacgacgatt
    11281 tgtgttttaa atttgcaggt ttaaatcatt acaattatca caaaatatgg aatagagaag
    11341 gggaagatat aacagaacag ttaataaaat tgttttatag ccctgattct actgcaaatc
    11401 ccgaagcaat aaaaaatatt tcggatttta aatttaattt tagcagttta aaaggtacgg
    11461 gttacctacc gtcaccttat ctgcgatatt attatttgac aaacgaaatg cttgaggaag
    11521 atttagaagt ttacaaaaat cacaatgtta gaggggagga agtaaagaga attgaagata
    11581 cattatttga aatgtacaaa gatccaaagc tgaaccacaa accggaattg ctttcaaagc
    11641 gtggaggagc gttttatagt gacgttgctt gtgaagtcat aacatcaata ataaatgatt
    11701 cccgaaaact tatgtccgtt aatacaaaaa acaaaggttc aataactgac ttagattatg
    11761 atgcggtcgt tgaggtaaca agtgtaatca cctctcatgg tcctgagcca atttcatggg
    11821 gtccctttac gccaattgct aaaggactat tgcaaaatct taaagcaatg gagattttaa
    11881 ctgttgaagc tgcccttgaa ggaaattatg agaaattagt tcaagcattc attctttgtc
    11941 caattatcac cagtggtggt aaagctatcg acatgcttaa cgaaatgctt gttgcaaaca
    12001 aaaaatattt gccaaatttt gcagaaaaga tagaagaaat tgaggctgcg aagcgttaat
    12061 ttatattggt caaaatttta aacaataatg tgttgattac gtcaaacagc gaacacttaa
    12121 tttttcgata tctgtgacat aagagaaagt tattgcccca ccgtgttggg gtgatagctt
    12181 tttaaataga ggccatgttg tttctaagga tcattgaaaa ttcttggcga gcttacaaaa
    12241 gtcggtaatt caccaaagga aacatttcct ggcgaataaa ctcatccaat aagaaaaatt
    12301 aggcaaaaca tcatataaat gacattcatg cgatcagaaa ggtctgcttg ggaaagcctt
    12361 ttttgcactg actattttgg attccagaaa gtcgtgcata gtggatattg caattcaaaa
    12421 ggtaaccatt tgataggata tgcttaaaat gaagatggat tcaccaattg ccaacttgga
    12481 aacttcccac aaaacctgaa aggtagattt taaaggaaaa atttttatat aatgaactgg
    12541 gaggaagtat gcaggaacct aagaatcaat ttcacacaat tgatgaatac attgccggtt
    12601 ttccggaaga agtgcaaaaa ctattgaaag aattgcgagc aaccatcaaa gccgcagccc
    12661 ccgaagcaga agagaagatc agttaccaga tgcctgcttt tgcccaaaac ggagtcctgg
    12721 tttattttgc tgcccataaa cagcacattg gtttctatcc tacttcgagc ggaattgaga
    12781 atttccgtaa agaattatca gcttatgtaa catccaaggg tgccattcaa attccctatt
    12841 cgaagcccat tccgtatgac ctggtcaacc gcattgtaac tttccgtgta caggagaatt
    12901 taaagaaagc agaaaacaaa ggaaccaaca gaaaaaaggg cgcttgattc tgtgaaattg
    12961 gtttgcataa aattaataat tattgatttg actaccgagt gagaaatcta tgaaaaagat
    13021 gaaattgaat ggagaggttc ggaccgctct tgtttacttt gtgctcggat gcatctggat
    13081 atttctcacc gatcatttcc ttgccttgtt gatcaccgat tcgtcgactt tgaccgatgt
    13141 ccaaagctat aagggctggt tctttgttgc agcaagcaca tttctgatct actttttgtt
    13201 gaaacgatat ctcgcttcca aacggcgagc tgaactgaaa cagatcgaaa gtgaagaaca
    13261 atatcgcctg ctttttgaga caagcctgga tgcggtcttt ttaacatctc ccaatgggaa
    13321 gatactcgca gccaatccgg ctgcttgcaa gcttttcggg cgatcggaaa aagagatctg
    13381 caatttgggt agaaatgcgt tggtcgatac ctccgacccg cgtcttgctc cagcgttgga
    13441 agagagaatg aaaaatggtt acttttcagg ggtgttgacc ttcaagcgaa acgatggaac
    13501 caaatttgaa ggggagatct ccactgcgct ttttaacgac agcaatggaa acctgcgaac
    13561 gagcatgatc ctacgtgata tcacggatcg caaacgagca gaagaggtga ttaaaaaaaa
    13621 acagcaggag ctccaacatt ttattgaaca tgtacctgct gccattgcca tgtttgataa
    13681 ggagatgaga tatctgtgcg taagccagca attcttctca gattacagaa tcgaagaacg
    13741 ggatattatc cataaatccc attatgagat tttccccgag atctctgaaa atgtgaagga
    13801 aattcaccga cggtgcttaa aaggagaaac gatttccaat gatgaagaac cgtttccacg
    13861 tctggatggg agggtagatt gggtgcgctg ggaagtgctt ccctggtatg agaataatgg
    13921 agagattggt gggattatcc tgttttctga agtgatcacg aaacgcaaat tggttgaaga
    13981 gcgtataaaa acgcagttgt atcggttgaa aacgttgcgc gagattgacc aggctatcgt
    14041 tgccagcaat gacatacacg tggtgttgaa cacgttgata gagatgctgc tctccagcct
    14101 gcgggtggat gcggtggatg tgctgctgtt ggatcctgcg accaatacat taacctttgc
    14161 cgtggaaaaa ggtttcctca ccccaaagtt agaaaaattc agtgttcatc tgggaaccgg
    14221 attgctcgga cagattgcac tgggacgcga gagattgttt gttcccaatc tgcgcgaaac
    14281 ctcccaacaa ttaagccgcc cctggctcct tacggatgag aaatttgtat cttatggttg
    14341 cgcacctctt tttatcaaag gagaactgaa gggggttttg gaagtttttc accgttcgct
    14401 gctcaacccg gatgcagaat ggctcgattt tttggattcc ctggctgggc aggcagccat
    14461 cgccattgac aatgccaggg tcaatgaagg gctacgttct gccaacctgg atctaaccct
    14521 ggcgtacgac gccactctcg aaggctggtc gcgcgcgctg gatatgcgcg acaaagaaac
    14581 cgaaggacat accgaaagag ttacccaatt cacgctagaa tttgcgaaaa ggctgggcat
    14641 cagcgaagag gaacgcgtcc atatccggcg tggggcgctg ctgcatgata ttggaaaact
    14701 gggcattcct gaccatatcc tcttgaaacc cgcagaactg accgaagaag aatgggagat
    14761 catgcgtaaa cacccggtgt atgcttttgg gatgttggcg ccaatcgcat atctgcgacc
    14821 cgccatggat attccctatt gccatcatga aaagtgggat ggcagcggtt atccgcgcgg
    14881 attgaaggga gaacagatcc cgctggcggc gcgcctgttt gcggtggtgg atgtgtggga
    14941 tgcgctgcgg tccgaacgtc cgtatcgaaa ggcatggcct gaagaaaaag cactggaata
    15001 tatccgtgcg caatccggca gtcatttcga tccaaaggtg gtggaggttt ttctccagat
    15061 gatggcagaa taaaagaaat ctgctcaaga taaacaattt aacatcagcg gcatcaccaa
    15121 aaaccagcaa atcatcagca aagcctagtg cggtaatcgg ttggattgct tttcaacaat
    15181 ccctctccct gtgacttcca gcgcgaagat ctgttgttta attaaggaca ttaaactgac
    15241 catgcgtaac gaaaaagaga tgtttgacct gatttcgcaa accgctcagc aggatgagcg
    15301 cattcgtgca gtcatcctgg aaggctcgcg cgccaacccc catgcttcta aggacatcct
    15361 gcaggattac gatattgtgt acatcgtgag cgaaatggag ctcttcgtgc gggatcacga
    15421 gtgggtgaag tgcttcggag agttgctcat catgcagata ccggatagca tgggagacca
    15481 accccacagt acagaagatc attttgccta cctgatgcag ttccgcgacg gcacgcgcat
    15541 cgacctgagt ctgtacacgc tggaggcatt ccaaaagcgc gggcgtgaca gcctgagtgt
    15601 gctgctgttg gataaggata ccacttttga atcgttccct gtggcggacg aaagcgatta
    15661 cctgcctcat ccgcccacct ctaaggcgtt cgatgattgc tgcaacgagt tctggtgggt
    15721 gtgcccctat gttgccaagg ggctatggcg cggggaaatc acctatgcca aatacatgct
    15781 ggatgtgatt gtacgtgcgc aattgatgaa actgctgaac tggtacatcg gcatgcgtac
    15841 tgagttcaaa tgcaatccgg gaaaagaagg gaaatatttc caaaagtatc tcagcacaga
    15901 agaatgggaa aaattgatgc gaacttatgc ggatgctgac cctgaaaaca actggcgtgc
    15961 tctgttcaac atggcagacc tgttccgggt gatcgcgcag caggttgctg agcattttgc
    16021 tttggattat ccaaatgcag agtatcagca tgtgctggtg tacctaaaac aaatccagaa
    16081 tatgccaaaa gataaaaaag aacttttttg accaatcttt tctatctgtg gattactttg
    16141 ctcgcgatac gtagagagaa gtgctgtgat caaaaaagcg atagattgaa ggaatgaaat
    16201 gacaaatgtt ttaacgttaa gaaatgtgaa acaggaagat cttgcagtgt tcttcgccaa
    16261 ccaaatggat gaggatgctg atttcatggc ggcttttact tcccaagatc gctttgataa
    16321 aaatgctttt gacgcgcatt ggaataagat catgcatgat cccactgttc acataagaac
    16381 aattctcgtt gatgacgggg ttgccggtta cgtgttgagc tatgaggaag agggaagacc
    16441 agaggtgagt tattggttag gaaaggatta ttgggggaga ggattggcta ccggtgcatt
    16501 gcggattttt ctgcagcagg taaatgcaac ccgccctatt tatgcccgca cggcaaaaga
    16561 caaccaggct tcacgccgcg tgctggagaa atgcggtttt aaggtgatcg ctgagagcga
    16621 aggtttttcc aatgcgcgtg gaaagaaaat accagagctg ttgcttgtgc tttgagccga
    16681 ttcgataagt ggaagaacgt tcgatataca aagatggttc gctatagcca ttgagcattt
    16741 aagctctcct acattctgat aaaaagtcct ccaatcttta ataatggatc agaggaacgt
    16801 tttttgtatt gtatcgggtt gttttttata tttaatcctt ggcgtgcaat ttgttgctga
    16861 tgaacttacc cacttccacc actgccagca aacctgcggc gataccggca accatcaacc
    16921 agtcttgcag ggttaatggc acaataccta gaaggttgcc aatggcgggg atgaggaagg
    16981 gtaatatggc agtcaccaac ccggcaccca ccgcccacaa cagcggtttg tttgatgtaa
    17041 ccttgttcat gcggaagagc ggaatgcgca tactgcggta ggcgaagatg tagatcatgg
    17101 agttgatggc gaaggatgcg aaaacgatgc tgcgtccttc gacaacattc aaatgcacgg
    17161 tgtacatgtg attgaaaaga taaagtgcag ccgcagcact ggcagtactg atgatgccga
    17221 tcagtgaaag ccccagatta ttcaaaatgg gttctttcag tgaacgtggt ttttcatcca
    17281 tgatgccccg ttctttgggt tcgtaaccca acacgatgtc agaaggacca tcgcagatca
    17341 ggtggatcca caggatctgc gccaccacca gcggagctgg ccagcgcagc atcatggcaa
    17401 tgaagatggt caacacttcg gcgaacgaat tcgagagagt gtatgccacc actttgcgga
    17461 tgttctcaaa gatgatacgc ccttcttcaa tggcagctac aatggtgcgg aagttgttat
    17521 ccagcaggat caggtcagcg gtttcttttg ccacatccgt ggcgcttccc accaccacac
    17581 cgatattagc ctgctgcaga gcgggcgcat cgttcacgcc gtcgccgatc atggcggtca
    17641 cttcttggtt attttgtaaa gcgcgcacaa tgcgcaactt gtcgtgcgga cggatgcgcg
    17701 cgaagatagc ggtcttttta acccgttctg ccagttcttc atcgctcatg gcttccaggt
    17761 cggtgccttc gatgatctca tcgccagcgt gcatcaatcc gatggtgcgc gcgatgcgtt
    17821 cggcggtcac gcggtaatct ccggtgatca tctttacccg gatgccggct ttctgggcaa
    17881 ccctgataga ttcctgcacc tgttcgcgca ggggatcttc catgcccagc agtcccagcc
    17941 aggtataacc gctgtggtct tccggctcac caccaccccg gtaagccaga cccagcaggc
    18001 gcaatccatc tcccgcccat tcttccaccc gaaccaggat gcgttccttt tcttttttct
    18061 ccagcttgca catttctagc acaatttccg gagcgccctt caggtagttg tatttttttc
    18121 catccaggtt gttcacagtg accatgaatt tggtttcact gctgaagggt tcttcagaca
    18181 ggcgttcggt tcggtcaacc agtttctgcg gatcatccga agccaatgtc agggaatatt
    18241 cccacagggc tacatccacc ggaccttcca gatcgttgca caataccatg gtctggtaag
    18301 cgcgcgtctg gtcagtgaga tcggcgcgtg taacgcgcat gcgcccttcg gtgagggtgc
    18361 cggttttatc ggtgcagatg acagtcacgg aacccagcgt ctccactgct tgcatgcgtt
    18421 tcaccagacc attgcgcttg aagatcttgc gcatgcccag caccagaata acggtaacgg
    18481 caatcagcag accttccggg acggcagcaa tagccaggat gatcgacgtt cgtaaggtat
    18541 ccagaaacga gccgcccagc gccaaaccgg cgatcaagat cgtcagggta aaaccgatca
    18601 caacataggt cagagttttg ctgaacgctt tcagccgagc ctgcaagggg gtatcttctt
    18661 ccacatgttc ctgtaaactg gcggcgatct tgcccagttc agtgctgctc ccagtgccgc
    18721 tcacttccat cagtccacgc ccactcaaga cggtggtacc catgaaaact tggtcgttcg
    18781 caactttggt gaccggttca ctttcaccgg tcaggatggc ttcatcgatg gtcaccctga
    18841 cgctttcgag cagaaccccg tcgccggggg ctttctcacc agcgttcaag atgaccaaat
    18901 cgcctggcac cagttcccaa acttctactt cgcaacgttc tccggcgcgg atcacggtag
    18961 tggtaggttt gagcaatccc ttgagagcag tgtaggtttt ctgcgcctga tattcctgaa
    19021 tgaatcccaa aattacatct acgatgacca cggcgataat gatggcgaaa tcgctggtct
    19081 cattggcgag taaggaaacc agagcagcca ccagaatgat atagatcaac gggctcttga
    19141 actggttgag cagcaagacc cagggtgggg ttgatttttc tgccggcaga cggttttcac
    19201 cgaactgacg gcgccgttcc aaaacttcag ctttggttaa ccctgatgaa tatcgtttat
    19261 ccgtttttga catgatcgat accttgcttg aatagggcga tgatatgcgg gtcaactacg
    19321 ctgtaataaa cttccttgcc atcgcggcgt gcctggacga tatgcatctg gcgcaatccg
    19381 cgcatatggt gcgaaacggc ggattctgtt acgccgatca gcgttgccag cgcctggctg
    19441 ttcaattcct gttccacgat cacggaaagg atgcgcaccc ggctggtatc gctgaagacg
    19501 ctgaacagct cagctacatg tgcagccgtg tgttcatcga gaataaaaga gtttgcattc
    19561 atgattattc cttaaacaat taaacatata ctcaattgtt tgtgtataat atcatcaact
    19621 tcattaaaaa acaagttttc cgaagaataa cggttttgct gtaaattcaa acgaaagttc
    19681 ccaggcaagg aaaaatgcag tgtatcggtg tatgggcaaa gcatagaagt tgcttcccat
    19741 cctgatattc agttgaggca tgcggcgtat ctggtggtat gccccgaagg tgtgcagtat
    19801 tctgcaatga atggcggaag agcgaatgag cgcattcaaa catcactctg cctgggttcc
    19861 acggtggaga ccgaaattga agagaaacaa gccactttaa tcgttgtaga taatgacccc
    19921 gccctggatc atattttgcc ggatggtggc gctatccggc tatcattcga tccggttcgc
    19981 tgttatctgc tgccgtacga aacctgattt cctgaaaaag cggttttgaa aaaatagtga
    20041 gttctggact ggaaatataa aaacagaacg gataacaata ccaaaagatt tgataaaagc
    20101 gctcgatcta ccacaagcgc ttttatcaaa taatgctcta atatcttctg tagatcgtat
    20161 cgatataata acaatgatag aagattaact tgtgcttctg gatcatggtt caggtagttt
    20221 gcgtgagcag tacaaagaga gaggagatcg attgcaatga aagggtttat tcaaaacatt
    20281 gaagacatca ccgtaaagaa tgagaatttt cggcaggtgt tgtataccgc ccaaaactgc
    20341 cagcttgttg ttatgtcttt gaacccccaa gaagagatcg ggatggaaat acaccatctt
    20401 gaccagttct ttcgggtgga agagggcagc ggtgaagcga tccttgatgg ggtaaccaca
    20461 cccatcagcg cagggtttgc tgtgatcgta ccagccgggg cgaaccataa catcatcaat
    20521 accggcaatg taccgctgaa gctgtatacc atctatgcac cccctcatca tcgcgatgga
    20581 gtgatccacc atactcgcgc cgaagcggaa catgatgaag aagagttcga cggaagaaca
    20641 acggaataag aataaaaaat tcccttggct acaataagca gcccttttct atcaggaaag
    20701 gttcctgcag gcatatctcg atcacttcac gtatactgaa taggagcgga gttggtacaa
    20761 tctgcttgcc tcatgagaaa agatattttg ctgacggcaa aaatatccga gaaccctggc
    20821 tatgcacttg ccatagcgag aatttgccca gactgtgagc ttgaggttat tgcgttgcat
    20881 agttctgcat gaaagaagtc gattttgaga atatgaacga accggagcct acaataaaag
    20941 aaaataaaac agagatgacc gctcaagacg tgcttgcgtt tctctctcta atgaagcaaa
    21001 acaatattga aatatggatc gatggtgggt gggcggtaga tgcattgctc ggcagccaga
    21061 gccgtctgca tgaagacctg gatatagcag tgatccatcg atctgtgggg cgcatacgag
    21121 cattgctgga agcgagcgga ttttcagaaa tagagcggga tgatagctgg ctgtgcaact
    21181 ttgtgatggg tgatgaacag gggcgtctca ttgatgtcca ctcttgcgaa ttcgatccgg
    21241 aaggacgttg catctttggc gtacactacc catacgatgc gttaaagggc agtggaatga
    21301 ttggcagtgt tccggtgcag tgtatcacac ccgagtggct ggtgaagttc cacatcggct
    21361 acacgctgga tgaaaatgac tatcgggatg taaaagccct gtgcgacaag ttccatcttg
    21421 aaattccgaa agaatacgca gactttggca aggtagaaga gggaaaaagg aaagattgaa
    21481 ggatgtccaa acagcacact ttcaaagcaa agatagaaga cgcaggaaca ggcggagcat
    21541 tcgtaaccgt cccgctggat gtggaagccg tctttggtaa gagtcgtgtt aaggtcaagg
    21601 cttggatcga tggagaaccc taccgtggat ccctggtgcg aatgggagga caggctcaca
    21661 tcttgggcat ccgaaaaaac atccgaaccc gaatcggaaa atcgatcggg gatgtggtag
    21721 aaattattct gcaagaagat accgaaccac gcatggtaac cgttccggaa gatctgcaga
    21781 acgcttttgc cggtcataaa gatgcatggg cgttctttga cacgctttct tattcgcatc
    21841 aaaaagaata cgtgcagtgg atcgaagagg caaagaaagc agaaacgcgc caggatcgcg
    21901 tacagaaaac gatccgattc ttattggaga agaaaagtcg ccagtagctc atgagaggga
    21961 cggaaaatat gcagaacaac gccatgatag atatattagc tggggatgag ccgttccccg
    22021 cttacaagaa ggatttgatg ttatatgggc aactggtagg ttcctggtct gtgcaaagta
    22081 cctggtacga tcggaacgga gaaattaaaa aaaagcgagg ggaatggcat ttcggctggg
    22141 ttctgggggg acgcggcatc caggacgtgc tgtttgctga aggggcaact cctgatcaat
    22201 acggcacaac tctgcgctgc tacgaccctg ctttgaagat atggcatgtt tcctggatgc
    22261 agccgtatgg cggggaattc gtgcatctga tcggtagaaa agttggggat tctattgttc
    22321 aagaaggcag cggattggat cctgcccggc gagagcgctg gacctttcgt gatatcactg
    22381 caaattcctt tctttggttg ggagaagtct cttttgatgg tggaaaaacc tggttcctgg
    22441 aacaggaaat gcgcggtacg cgcatggaat gaagctatgc aaaaaattca ccatgaaatt
    22501 ttacagttgt gcttcaatag cgagtttcga cttcttgtcc agcagtcctc cgcttaaggc
    22561 gtgatgaatg taattgagga tgtcattttg ggaatttgct ttgttgatga gaagcaatac
    22621 agaaatgata tccagcagca ccagcttttg ctggctttct ttgcgctggg agatatcgat
    22681 cgacgtcaat ttatctgtga tcctggcgtg gcagtgggtg ttaaacggta cgatgctgca
    22741 tagcccctga atgcactggc gcaccgtgac cggtttttcg tcatcgcaaa acgatagata
    22801 aagatcgcag atttcattga atttcccttg cttatcccag cgcacatttt ccgcgatcat
    22861 gcgcagaccg atgttgcgct ggtaagaatt tgggtcgctc aatttcgtga caaaagtatc
    22921 ccagtagggg aacacatcct catattgttc agatctggca agcaacagca agaagcaatg
    22981 gtagcggagc gtgtccgctt tttctgacaa ccatggaacc agttgcgcga tatcgccctt
    23041 tgtgagggtt agagcgagtg cgggcaggtc ttcttttggg taagattcga tcttttcgcg
    23101 ggtgagcatg gaatttctcc tgagagtttt aaaaattata tactttccct gtctggaatg
    23161 aagctatgct ttatctgttc gatatgaaac cagtatcaga aatttgttga gaaaaaaggg
    23221 ataaaaatgt tttactccta aaatttactc aacaactgga aaaatttcca ctttccatgt
    23281 gaaaacattt cattgataat gaaaaataat tgctatatca tttccttaat gaatgaaaac
    23341 aaacggatgt ggaaagtcaa tttgctatga aaacgaatga attgatcatt gttcagaccg
    23401 ctgctgctga tcaagatgaa gcggaaaaga tcgcgacgct actggtatcg caaaagctgg
    23461 cagcctgcgt gcagatgctg cccatccaaa gttgttatcg ctggaaagga aaagtcgtgc
    23521 atgactctga agtgcttctg ctcatcaaga ccagaagtga tttgtacgaa gagctagaag
    23581 ctgcgatact ggaaacccac agttatgaag tgccggagat cgttcaagtt cctatcgagc
    23641 gtggatcggc aaattatcta caatggatcg ttgcaagtac caggaaggaa gcgtaaatgg
    23701 atgccatacg cgtattcctt gaaagcgcca gccacagggt cttttgcggt gcgctcgatt
    23761 ggccggggtg gtgccgcagt gggcgtgatg aagatgctgc tctgcaagct ctgctggcgt
    23821 atgcaccacg ttatgcagaa gtgctgcaga ttggcaacgt atggtttcct gaagtgaaga
    23881 acctctcgca acttattatg gcggagcgtg tgcctggaaa tgtcaccacc gattttggcg
    23941 caccggatgc aattctggca ggtgaccagg aagctctgga tgaaaaggaa acagcgcgct
    24001 tccaggcaat cctgtgggca tgctggcgca aactggagca ggttgcccgg gcagccgagg
    24061 ggaaaatatt gcgcagcgga ccgcgcggtg gaggcaggga tctgcagagt attcttgagc
    24121 atgtcatgga aagcaatgct gcatacttag cgcgcattgc gcataaagtt caacgttctg
    24181 ctcaagatga tctgctggcg gaactgcgca ccattcaaca ggcagatcag actgccattg
    24241 aaaatgctat tcgcaacggc ttgccgaaga gtggtccgcg cggtggacgc atttggacgt
    24301 tacgcttctt catccggcat gtcgcctggc atgttctcga ccacgcctgg gaaatcgaag
    24361 atcgtgttga ataaaagacc acctgctgca tattggttac agtaccccat aatcccctat
    24421 tgaggacgta tagaacaggg tcgggtattt ttaatgaaat gatgggaaaa agccaagtcc
    24481 ggctatgttc tttgcttgaa tgatttagaa tagtgtgaag tgaattcgtg atttaatttt
    24541 tgaacggagg caaccatgaa agccattgct ctggtggtca gcgccaggaa acagggaaac
    24601 tgctatgatt ttgctcaatt cgtcctggac gattacaaaa acaaaggtgt agaaacagag
    24661 ctgatcaatt tttatgacta tcagatcact ccctgccaac gttgtgcgta tgagtgcatt
    24721 caaaaacttg atccggcaaa aggcaccaat tctgcgtgcc cgattcagga tgatgtgcgc
    24781 atgatctggg aaaagacctg gcaggcagag atattgcttc tgtttatccc caattatggg
    24841 gggatgcccc cggcgttgtg gctggctttt tcacagcgtt cccaggcatt cttccggcag
    24901 gcacctgttg agaaattgaa acattcggta gtcagtgcgc tgatcgtggc ttccccgcac
    24961 caatccagcg gagcagcctg ggcgttttct ttcatggctg atgaggtgaa agggctagat
    25021 agaaaagtgg cgagtttcga agtgatcaat ccgccgcaat ttcaagatga atatacgttc
    25081 tctccgttga tgcaaaatgc cgagattcag cgccggttga aatttatggc agaacagacg
    25141 tggaaaaccg ccgtggcttt aaactaaaaa gttgttgcaa gaaaatcttg tgaatgaaga
    25201 acgattatga aaaagtggat gcccatcctt gtcattggat caatcctggt ttttacagca
    25261 ggtgtgatct atttttatgc tcactcgtcc tccgaggagg atgtgacaaa cgaagatgga
    25321 agtcagacac cttcctttga atcgatcgga aaaaccacac cggcaaatca gtcagagttg
    25381 ccggttctgc tcgcatacta taccggtgat gaaggttcat tcaaagcgtt gcaaatggcg
    25441 gctccgtatc tcaccgctgt ttcagcagat gtttatcttg ttcaagctga tggcagcatc
    25501 aacgggtctg acgagatgag tgcaatgact tggaatgatg ctcggaatat tgaaacatat
    25561 gcttgcatca gcaattacaa tgacgatcca gaagtgaatg attttgacgc cgaactggcg
    25621 cacgctgcca ttgtcacgta tcgcgaacag ttgatcgaaa gccttgtatc ggtggcgcgc
    25681 gatggcgggt atgcgggtat caatattgat tttgaaaatc tggcttattc tgcagatatc
    25741 gaagaagacc gctctgcttt cacctctttt atccagggtc tggctgagcg cttgcatgct
    25801 gaatcgctca agctgatcat cagtgtgccc ggcaagagcg aggaatcaat cgataacacc
    25861 tgggcatatc catttgatct ggctgtgttg ggagaaacgg cagactatct tcaattgatg
    25921 acctacgacc agcatgggcc atggggagaa ccaggcgctg tggcgggcgc agattgggtg
    25981 gaagattgct tgcagtatgc tacgtccctg gtggatccgg caaagttatt gatcgggttg
    26041 cccgcttatg gctatgattg ggatctgacc gcttcggatt tcgaaaacgg cagcattgtg
    26101 gctggcgaca tttcatggaa ggattttgcc gctttggtca gcagacctga tgttgaaacg
    26161 ggttgggata tcgcttcgca atcaccttat tgtaggtatt ccgaagaagg gcatgcgcat
    26221 atcgtgtggt ttgagaatag ggagagcgtt caaacgaaag tgaaattggt tcgggaatat
    26281 gctctggcgg gtgtgtctgt gtgggcgctg ggaaaagaga atgaaaattt ctggcaggca
    26341 gtagaagaag ggttgaaata aaagccccag gctcttgact actcgtcacc cccgcaaccc
    26401 atggatgatt ttggcgagca ggtttgtgcc agcaagcgag aattgaaaag gaggtagcac
    26461 gcccgttgcc agcctgatct catcggcaag cgaggagttg ataaaattcc atcaatttgc
    26521 tggtcccatt atcctgatca ctgcaatgct tttgccacca tgggggaaaa aataattttg
    26581 atttcaacga tgctccactc tacactctgt ttaagagaca cgaatggaga atatagcgat
    26641 gaacaaatat taccctaatc tttttaaacc cttaaaggtc aattcgctta tgttgaagaa
    26701 caggatattt tcatcttcta tgggaatgcc tgcacaccat attttgcttt cttcaacata
    26761 ctatgacgaa gtaagcctcc ttgacaaagc catgggagga gccgcactgg tttttcttca
    26821 gcccgaaaaa gccattgccg atgataattg cgagtatccc aaatatgaaa gagatagaat
    26881 ccgcgaggat ctttcagtag caaggcaata cggagcgaaa gcagctattc aggtatggcc
    26941 acgattgaag atcaacacag gcgatagctt gcgcgctcat tatgatggga tggtagtgca
    27001 tgccccgtcg ccatatttaa caaagagcgg acagcgtgcc atcgaactga ccaaagaaga
    27061 gatcaaaatg atgatccagg aagcggtgaa ggatgccatc gccgctaaga atttcggatt
    27121 tgatatgatt caggttcggg tgggcgctga aagcattgcg gcgcaattct tgtctcccgc
    27181 tttcaataaa cgaacggatg aatatggcgg cagtctggaa aatcgtatgc gattttcgat
    27241 tgaatatatt cgggcagtgc gagaagcggt cggagcagat tatcccatca tgttccacgt
    27301 cggtgaaacg cagttccttc cgaaatctta tggagccgag gatgtttatg cgctgcttga
    27361 taacgtcaaa gagtatgttg atatgatcaa cgcggttgct ggagggatgg atatgatccc
    27421 cggatacttc ccggaagata aaacctttga cccagccatg gggttcaaag cctggtatgc
    27481 tgcaaatggc gtggcgacct caaaatttac ttcaaccgaa tcccttagcc tgatctttgc
    27541 tgcgaaagta aaagaaagat atcccgacaa gatcgtgatc acgaatggct cgatcatgac
    27601 cccagaaaca gccgaagcgg caattgcgga aggaaaagtg gatgggatca tgcagggtag
    27661 ggcattaaac gcagatccat tctggccaag aaaggccatg gcaggacaac gggaggatat
    27721 tgtcccttgc ttacgatgcg ggtattgcta tcacacggca accaatcaca ccaacacgca
    27781 atgttcggtc aacccgcgct accgcagaga aaatcgggtg ccgttggaag tgcccaaagc
    27841 caaactaaag aaaaaggtgg tggtggttgg agccggaccc gcaggttgca aagcagcatt
    27901 ggtggcgcag gagcgcgggc acgaagtgat tctgctggaa aagagcagtg aaatcggcgg
    27961 gcagatccgt tatggtcaat atgaaaagat caaggtagat ctggtgaatt atttgaacta
    28021 tctgaaggcg cagatcaaga aaaacaacat tgacattcgc tacaacacca ccgccacacc
    28081 ggaactgatc agggagctca aaccggatgc tctgttcgtt gcaatcggtg ccaggcctat
    28141 taccccagac atcaaaggtt cgaacggaga gcatgtgcat caggtgttgg atatttaccc
    28201 gcaactcgcc agattgggga agagagtgac agtgatcggt gggggattgg tgggctgtga
    28261 gctggcgtac gaattgctgg agaagggaca tgaagtaacg attgtggaaa tgactgatca
    28321 aattgcagca cagggacatt tcctctatcg catggacctc gaacgaggcc ttgagaaaca
    28381 tggagatcgg gtaacgatcc tgaagaatac tgtatgcaaa gaaatccagg gaaccaaagt
    28441 gctcgttgag cagggaggtg agaaaaaaga gatcgaaggg gatgacgtgg tgatcgcggt
    28501 ggggatgaaa gcggacaaag aagaggcgtt cgcgttctac gggatcgcgg acgaaacgtt
    28561 catggtggga gactgcgaaa aggtgggcaa ggtgctggaa gccacgaacg acgcgtattt
    28621 catcgcggct aatttataag aatagaaaaa gcttattggt atggtaagag aagaaccggt
    28681 gttacagccg gttcttctgt tttttcctga attttatgga aggggttttt gccactatgg
    28741 tggaaaaagt agaatggcat ttgaaattca caaaaaatat aatggcataa atgacttcat
    28801 aagaacaacg aacagagatc aaaaggttgg attcatgaca aaacttaata aaaattttct
    28861 ctgggggtgc gcatcggcag cgaatcaatg cgaaggcgct tatctagagg atgggaaagg
    28921 acttagcctt gcagattgtt taaccgccgg gacaaaagag cgaagaaggg aatacacaga
    28981 tggggtggaa gagggcaaat attacccatc acacgaagcc gtcgattttt atcaccactt
    29041 tcgggaagat attaagcttt ttgcagagat gggaatgaag tgctttcgca catccattgc
    29101 ctggagccgc atatatcccc atggtgacga tgatgaacca aatgagaaag ggcttcaatt
    29161 ctatgatgat atgctggatg aactgttgaa caatggcatt gaacccgttc tcactattac
    29221 ccactacgaa cctccttacg cgctggttga aaaatatggt tcctggaaag atcgtcgctg
    29281 tattgatttg tatgaaaagt actgtcgtac gattttcacc cgctacaaag acaaggtgaa
    29341 gtattggatg acctttaatg agatcaattc agtgatcttt aacccgacct ttccggtggg
    29401 catcagaatt ctccccggcg aaaataaaaa agaaattgcg tatcaggctg cgcacaatat
    29461 gttggttgcc agcgcgcgcg ctgtgagatt agggcatgaa atcagccctg atttcaggat
    29521 tggctgcatg atctgtatgc ccatgttcta tccggagacg tgtaaaccag aagatcaagt
    29581 tgaagctatg aaagccaacg atgacacata ttttttcagt gatgtgcatg taaggggatt
    29641 atatagcagg aaaaccaaaa gattgttcga aaagaacgac ctgcatatca acatggaaac
    29701 aaccgatgag agagatttag aagaaggaac ggttgatttt atcgggttca gttactattc
    29761 gtccgcagtt tccaccagcc gtgaagtgga caaatcacag ggcaatatct tttggatggt
    29821 gaaaaatccc tatctggagg aaagcgaatg gggatggggg atcgaccctc aggggttgcg
    29881 cattgcgtgt aattgccttt atgaccgata ccaaattcct cttttctgtg tagaaaatgg
    29941 gtttggtgcg caagacacgg tggtcaatgg acgggtccac gatccatacc gcattgatta
    30001 tctacgcaag aacattagcg cattgatcca ggcgattgac gaagacggcg ttgacctgat
    30061 cggttacaca gtgtggagcg caacggatat cgtgagcgca ggtacaggcg agatgagaaa
    30121 gcgctatggc atgatctacg tagatcgaga tgatagcggg ggtgggagca tgcagcggat
    30181 aaagaaggat agtttctact ggtaccagaa agtgatcgca tcaaatggcg atgacctgga
    30241 gtagctcttt tttttgccac aatggcggca atacacaatt tgaaaaaaga acaataaaaa
    30301 ctaccatcga gttaaaagca gatatattac ggcttcattg aagttgataa aaataatatg
    30361 ggtaaaggag gcttttatcg aatctgaata ataacaataa ggaatatctg aaatttaaaa
    30421 aggagataag ttatggaaaa gttcacgaaa tttttagaaa aatacttgga acctgttgcc
    30481 gaatggatat ccaataatcg gttcctaaaa tcaatttcaa atggcatgat catgctaatg
    30541 cccttcctcc tggtaaccgc gctgttctct attgtgcaag gagtgcctgc ctggttagat
    30601 ttcattcctg attggagcga aggggtatcc aatatactat tgttgccgta caacgtcacc
    30661 ttcggattga tcgcgttgat catttccttc acggtcgcct ataaccatgc tgagaaatta
    30721 aaaataaatc ctgcaaccgg tgggttgatc tcgcttgtcg ttttcgttat tctctcggca
    30781 aaatacgaaa atggggcgtt cgacggaacc tttttgggaa gcagaggcat ctttacagcc
    30841 atcctggtgg ctctcctggt aattgaactg tacaaattct tattgaaact caatctcaaa
    30901 ttcaaactgc ccgaatcggt cgcgcacacg gttcaatctt cctttgaagc catcatccca
    30961 ttggctgtca tcttggttgt cttctacgga ctcagtgtat tggttcagac aacaaccggt
    31021 caaatgatcc ctgctttgat tcagagcgca gtagcccccg ctttgaaagc cagcgactca
    31081 gcattgttct ggttcgtatt gggaatcttg atgagtgttt tcttctggat gggtatgcat
    31141 ggttgggcag tgatgtctgg cgtatggttg gctgccacca tcgccttcac ctcagcaaat
    31201 gccgaagccg cggctgctgg tcagcccatg ccctacattt ctggtggtac gatctggctg
    31261 tgtggtcaac ttcattggca tgtgccgttg atgttaatgc tgttctgcaa atcggaacgc
    31321 aataaagcta ttggaaaagc cacgttgatt cccggtattt ttgctctcag tgaaccgtac
    31381 acctttggtg ttccggtagc cttcaacccg atccttgcca tcccctacat tttcgccggt
    31441 cctattgcca acgtcctcaa tttttatgca gtaaaatggg gcttaatgaa tcgcagtgcc
    31501 aatctgatcc agatcctggc tcctgcgcca cttcaaggat ttcttggaac caatggagat
    31561 tggagagtat tcccgatttt ccttgtcctt ttggctgttg aagtggtcat ctggtacccc
    31621 ttcttgaagg tatgggataa taaatgcctt cgcgaggaaa aagaagcagt tgcttctgcg
    31681 aaaacgaaat agcataacag tggcgaaacg aagcacgata taccatgaaa actataattt
    31741 tattttgtgc aaatggtttt tcaacgatta tgatgcgaga taaaattcgc gaaacatcgc
    31801 aaaacgaagg gctggattat actattgagg cattcccttt cgctaaactg gaagaagaag
    31861 gacagcgcgc ggatttaatt ctcttggcac cccaaatcag gttcaatcga aaggcagtcg
    31921 aaaaattgta cccagacaaa aaaattatca tcgtggatat ggctgcctac ggagccatga
    31981 acgggagtgc tgttttaaaa gaaattcgaa acacactggg tgattaaaga gtatgccatt
    32041 ggagattcca ttgaaagagg aaataacaaa tattagctgc gagatcatcg ccctggcggg
    32101 gagtgcaaaa agcgctttca tcgaatccat acgggcagca aaagatggcg atcttaataa
    32161 agccaaggaa ctgttcgata taggaagcgg acaattcttg caagcccatg aaattcatgc
    32221 caacctgatc aaaaggtttg cagatggcga tgaaatcgaa atgaacattc tcctggtgca
    32281 tgcggaatgc ttattgacca gtgctgaaga tttcaaggta atctctcgcg aaattgttga
    32341 atttgcagaa agaaataaaa aatagtagaa gtcatcaaac acaaatgata aaactggctc
    32401 ttttctatgc cagttttatc attcttttca gaaatattaa caatgttatt attgtctgca
    32461 catcttgtga ataaaaacaa agttttgcga tgaacctaac tgtacgacag tatcagatca
    32521 tacggttgtt gaaagaaaac atcagtgcat tatcaggcaa tcaactttgc agtgaattag
    32581 atgtatcatc cagaaccctc cgttatgaaa taagcaggat caataaatat cttggatgcg
    32641 acttgatccg gtcgagccgc gaagggtata gcattgatgc cagcctggat ttatccacca
    32701 tattcaacga tatccggctc aatgattacc tgtatatcgt aaaatcggta gcgattctat
    32761 gtttagaaga aaaggatctg tcagtttacg atatcgaaga tcaatgcttt gtcagtaaac
    32821 cagtcataac gaacatgctg catctgatct tgcatcagat gaacaaattc gatcttcagc
    32881 tctatagaac aggcgatttc atttcagtca aggggaccga attcaacaag cgaaggtacc
    32941 tcattaattt gttattctcc gaaacatttg atgaatcgac attttgtccg gaatcaaaaa
    33001 actattttac cggcttcgaa ctttcagacc tttgggagtt ggttacaagt actatagaag
    33061 acatcggtat tcatatcaac gatgtttacc tgaaacacat tgtcaattgc acagcagtca
    33121 gccttcaacg tatcatgaat ggtcacatta ccgagaaaca tgctgctgcg gttgtcaatt
    33181 ttggtgaaga gcatgaagca tacctttttc taacctctct tctcgacaag gtgttccata
    33241 aatacaaggt gaaagtcctt gaagaagact ttcggagcat gttgtgtttt tatgcgggat
    33301 ctttcagaga taccaatgcc aatcccctgc gtatgacgat actcaataac aaggaatttg
    33361 aaattacgat tcgcagaatc atggatagga cactcaagca gttcaacctg catgcgaatt
    33421 acgagccatt ctttgatcgg cttttattgc atgtttattt tcttttactt cgcgcaaaaa
    33481 catcctcatt cgtaaaaaat gatattacct caaccttaaa gcagaccaat ccatttattt
    33541 acgatgtttc tgtttttatg gcatatctca tccaaatgga attcaaaatt gaaatacctg
    33601 atgaagagat tggattcctg gcgatctatt ttggttctgt gatcatcaat accattaatt
    33661 attccaattc ttacacggtc atctgtgttt gcccgaacta caacaatctg cgcgaacaga
    33721 ttgaggccca attactgagt atgttcaata agcgcatcaa tatcgtaaag tatgtttcat
    33781 cgtattcgca tatttcagat gacgatacct atgactttat tatatcggtc atcagcaaca
    33841 gctatacctt taaaaatatc gtgtatgtca gccctgtaat cggtccgtgg gaataccaaa
    33901 agatcaatgc aatgatcatt gaatgtgaaa aaaaacggca aaaggaagag atcaaagcgt
    33961 tggtactgaa gtatttccag cctgatctgt tcttttataa cgagccttat cttaaggaca
    34021 tggaaattat gcaatttctt catagtagaa tgaaggacaa aggaatcgtc gaagatgact
    34081 ttttggatta tgtattgaaa cgcgaggaac ttgccagtac agctttcttt aacaaattcg
    34141 cgatccccca cgcgatcaac gcaaatgcca gagacaccaa ggttgcttat tactatagcg
    34201 ataaaccgat ccgctggttt ggcagtgatg taaacctggt attgcttatg gcaaccaaac
    34261 agtatgataa aaattttatt cgtctatatg aattgatatt tgcgattttg atggataagg
    34321 aactgtattg caaattgctg aaatgcaatt cctatgaaaa aatggtcgat tttatcagca
    34381 atcaattgta gcttttgtat atccgtatgg gaatcttttt gcttttcagg atggcattta
    34441 gggaaacaag gcaatggaac tgacaaaaac attgtatgtt cctgatcgga cagcctggca
    34501 tgcctggttg gaagaacatg ccgccagcga gcgggaggtc tggctcattt attacaagaa
    34561 ggacagcggc aagccgcgca tcccttatga ggaagcagta gaagaagcgt tgtgctttgg
    34621 ctggatcgat agtctggtgc agcgcattga tgaggagcgc tacgcgcaga agttcacacc
    34681 tcgtaagttg gggagcaagt ggtctgaact gaacaaacga cgcattgcaa agctggtcaa
    34741 agaagggcgc atgaccccgg ctggcatggc gaaagtggat tttcatgtgg acgagatcaa
    34801 tctcgatgaa cctgtcatct ccgggaaaaa gccaatgccc gagctatccc cttcgatcca
    34861 acagttgttc atggaagata atgtggtatg ggaaaacttc agtaaactga cgccctctca
    34921 ccggcgcaac tacatcagat ggatcatgga tgccaaaaaa gaggaaactc gcatgaaacg
    34981 cgcacaggaa gcgctggaac tcctgaaaca aaatagaaaa ttaggtatga aatgacatca
    35041 tcgatcgaac aattcacttt tcgttcacct tcaaaagacg atgccgaaaa ggtgggggag
    35101 tgattgaaga agaatggaga caaaaaaacc gcgaatggac aattcgcggt tttttatttt
    35161 atctgacctg ccacatctgc actgtcccct cgttggtaca ggtgagcagg cgcgtcccgt
    35221 caggcgcaaa ttgcagggag ttgacaaatc cgcttgcggt gtgaatctca ccgataaact
    35281 gagaatttac tacatcccag aacagcacct tgccatcact gccggctgcc agcaggctgc
    35341 catcagggga aaagttcagc gaactgaaat agtctggatt gctgaaaacc gccagcgcct
    35401 ccccgctgtg ggcgtcccac agggtgatga tgggcaaaaa ttccccatcc acgcttccgg
    35461 cggctgcggt ggcaagcaag gctccatcgg gagagagggc agaagcgctg atgaaatcct
    35521 catggctgat ctcaggtccc agggtcgcgg tatcaatatc cataggttgg atggtagcgc
    35581 gcgcgatcca tagaacggtt ttgccgtctg ctccgaactg gaaggaatag accggtgcgg
    35641 ctgttgtaaa acctgtgagt ttgtgaataa ttccggcgct ggcaatatcc caaattgtga
    35701 tctgccattc ttcggaactg gcaatcccca accattttcc gtcaggggag aaagctgcct
    35761 gctgcacaaa gacttcttgc ggaatgctga ctacttcctg ttgagccagt acatcgtaca
    35821 gaacgatgga tggctcatcc gatgcatacg cgaccaactt gccatccgga gaaacgctat
    35881 acaatgtcgc atactctccc acttcataag aagcgatctc ctggtaggtc ttgctgttgt
    35941 aaagggttgc cctggtaagc ccttgcaacc agaacgactt gccatccaga gcccaccgaa
    36001 cgttcaatgc ttctgctgat tgcacaacct cagcactaac aagatcagcg accgtatgtg
    36061 cgttgatctg tgccgcgggc ttacagccgg caaacagcag aacagctccc agaaatagca
    36121 aatagaggat ctttttgata ctgttcatcc gttactccaa tctccttctt tctttatgaa
    36181 acgtttactt tgcgttcatt gttcccaatt gagaatttta tcaagccatt ccatgaaact
    36241 ttccttcaac atggcgaaga gactctcttt cttttcgtcc agccattctt ccaattcctg
    36301 gctggctttt tctttttgct cttccacaat atcttctgcg ctgtttttta tcgtttttcc
    36361 ggcagaagtc agcaacccct gcatggaaag cgccaaccgt tccttcagat cggtgggggt
    36421 ggctttgatc agcaccggtc gttcacttag catgaaagcg gtctctccgt ttgtactcac
    36481 cgaaagctcc aacccttttc cgcttctgat cgaatcggca tcgagagaaa aagcttccag
    36541 ttgataacca cccacgttct gcagggtata tgccagaaaa tcatcgccac cgtacccgcg
    36601 ccaaacaaaa atatccacca tccctgcact ggtcatgcgg tattgataat agccgttgaa
    36661 gatatcgctg gcgtcttgtg aactgcggag tccctgcaaa ctggcagcca ggttcttgaa
    36721 cacttgctgg ctgaacaccc cgaaagaatt gtctgcggca ctgtcggttt ctgcatattg
    36781 ccagaatatt ttttctacgt tgtgccgccc gagcagcata atggatgccc ggctgagaag
    36841 gtcggcgcgc accacatcta ccagcgtacc acgctcctcg gcgatggcag caagatcctc
    36901 tgtctgccag gctaaaccag taatccagat gggtttctcg ccgtactggc tgccagcggc
    36961 atctaccagg gcgatgatat ctgccagggt aaaaccgccc tctgcgctgg tgaggcatac
    37021 gcccgatacg gtatcgtagg tttggtacat atacttccct tcgggtgcga tggagtacgt
    37081 gtcgagttca accccgatca catcaaaagc attccacatc ccaagggtat tgatgctgtt
    37141 taagaaagcg ctgggctggt tgatgcagtt gccggtatcg cttcgtatcc ctcccaatat
    37201 gacaagatcg ctgtgagatg cctctttgat ggcgttcgag gcaatcgcca ggcgttcggc
    37261 atacgtggaa gggctagcca gtacagccgc ggcaatgggt tggtcttgca gttcgaaagg
    37321 taaattcaca gattcaccga tctgccaggc gtcaaccaca tccccaaatt ctgccacgct
    37381 cgccctgaca taccgttccc agctttcaag atacagggaa gcgacgtttt cttcgctgag
    37441 attttcgtat tgcagatagg agggaaatcc ttcaagaaca agagtgatcg acattccctc
    37501 ccgctcaagt ttatggatca gatcacccag gtcgatctgg gttccctgtg agttgatgga
    37561 ccaggtgaac agaccggcag atcgttccac gaattgccag gggatggcga tcttgacaga
    37621 cgtgacccct agtgtggttg cggtgtcgac gaactgttca ccatcctgcg cattctctaa
    37681 ataagcgctc atgtccaaac caatgggtga agggctggta agggcatttg agcccttatg
    37741 caagaaaacg tatatcaccg ctgcaagaag agcaatcacc agcaggaaag aaaagaattt
    37801 tttcatatag acccttttaa ctctacatga gacgtagaag tactgtttca gttgcaatct
    37861 ctatgccaaa acacgggggt cgatccagga aaaacgcgat agaataaaac aagagtaaaa
    37921 atggcagacg agaagaatga acccctaagt cgtagcgtac cgcgttttgc cgtgttgcag
    37981 ttatctggtt gcgcaggctg cgaagtatct ttgttgaacg cacacgaatg gacggagaat
    38041 ttccaactag cctacatgcc tctggtctcc agcgcctatg ccgtgccgga ggtggacatt
    38101 ttgctcatca gtggaggagt gcggacagat gaagatgcat atcatctgaa gaacgcggtg
    38161 aagaatgcga aaaaagtggt ggcggtagga acctgtgcca tttcaggcgg ggttgccaac
    38221 cttggcaatc gcgatgaggt gcgacagctc tttttgcgcc ggcaagaccg tctggaagtg
    38281 ccacgtttgt tgccacgttc cagaccggtg gatgaactgg tgcccattga cctgtatctc
    38341 cccggctgcc ctcctacgcc ggagctgttc atggctgccc ttaccgatcc ggaaggatat
    38401 caagccagcc tgattgtgtg ccaggagtgc ggaagaaaaa aactgaaaga acccaagccg
    38461 gatcatctgc tcggttttca aacagggaag gtcgatccag agatatgcct tatcaaccag
    38521 ggttatgcct gcatcggcac cagcacgcga ggtggctgcc gggctccttg cacgcgcgca
    38581 gggcatccct gcgtaggttg ccgcggtcca tccaatctat tgatcgagaa ggactcacaa
    38641 gcctggctgg aatcgatcca aaaaatcttt catggtatga caaacgtttc tgatgaagag
    38701 ctcagagaag ccttgctttc gccccaactg gcgctcttcc tcttccagtt ttccgattac
    38761 gccggtggcg atcgtcctgc caggtcaaag gagaaagtgt tgtaatggct actctaactt
    38821 tccccctcag ccgaatcgaa ggacatgccc gggtggagat cgaggtgcag aatggcgagg
    38881 ttgtttccac ccgtttcaaa gcaaccgaga tgcgcggctt tgatattttt gtgcggggcg
    38941 ttccggcgga gcaaatgccg gtgatcgtcc cgcgcatctg cggggtatgt tccaccgccc
    39001 accatgtggc gtcggtgaaa gcactggaag atgcttatgg tgtgacacct ccgccgctgg
    39061 catccagaat tcgctctctg ttgctgctgg ggcagcttct gcaaaaccaa gctacatctt
    39121 tgttcatttt caccatgccc gaccgggtag gggtagatag tttattcgaa atggacgatg
    39181 tgcagggaaa tacagccaaa cgttttgaga tggcaaaaca agcgctcaaa atcagagaat
    39241 tgggcacaga tttgatcaca ctggcaggcg ggcaattcat ccatccggtg cgcgcggtgg
    39301 tgggagggat cactgcgggt atttctgcag cgaacgcaga acccatgctg aaacgcctgg
    39361 agcaggcgct gccaacagcc tgtgatctgt tcgatgccta ctgggagctg acgatacgca
    39421 tgaaggaccg cattggcagt tggggcgacg accaaccggc ttactacatc acatccaccg
    39481 gcaaagttaa acccgatttc aacagccatg agattcgagt tatgaacccc gccggtgaat
    39541 gctgcgagat cttttcaccc agatccttcc gtgatgtgct cgaattcaag gaaagcgaat
    39601 attcttatgc cggcacgacc tcatacaaag ggggagtgat ccgcgccaat tcgctggcac
    39661 gcgccaacct gactgcccat atgggcacca gggtggcaaa tggctacctg caccggttgc
    39721 gcattgagtt tggcaccccg gcgcatccca tcatgctgtt cgatctggga cgcggtattg
    39781 aactggtata tgccatcgag cgtgctgcgc aaatgctggc acagccactg gataatgagg
    39841 atacggatgt gccatacact ccacgcgacg gcgaagggta tggtctggtc gaggcaccgc
    39901 gcggaccgct gatccaccac tacgtgatcg agaaagacaa gatcgccaag gcggaattca
    39961 tcatccccac cgtgcacaac atgcattcca tcgagcgcgc tttgcaggtg gctgcgaaac
    40021 gctatatatc tggtgatgcg gtggacatgg aactggaacg tgcggtaggg cgggtggtgc
    40081 gggcttttga tccttgcatt gcctgcgcga cacattgata tcgttaacct ttctttaatg
    40141 tgcgctaagc ttgctttcca ccctaattca gatagaatga aaatataaaa atttttggag
    40201 gagatcatgt attatagcga ttgggaacca agttttggat ttttatttgc ctatgttctc
    40261 tttgttcttg ctctcgtggg tttctacctg tattgcacct ggcgtctctt tgaaaaaacc
    40321 ggacgcaaag gatgggaagg attggtgcct gggcacgaca cggtggaaat gttcgagatc
    40381 gttgggcgcc ccggttggtg gtttttcttg ctgttgatcc ccattgccaa catcgtcatt
    40441 gggatcatga tccagttcga gctggcaaaa gtgttcggca aggataccgg ctttggtttc
    40501 ggtctgctgt tccttccttt tatcttcctg cccattctgg ctttaggagg tgccgaatac
    40561 cagggtccat tgacccatga ggcgtaaagg atcgcgatgt gaaaaaagca gccacactgg
    40621 ctgctttttt gttagctcgc gctggctaaa gaaggcaggg tgattcgctc cagagtatcg
    40681 atcgttccca atctgttcat cgggatgttt ccgatgaacg gtgcaagcct ttcccttcta
    40741 tgagccgatg aaagatatcc atactcgtct gaatggttta agtataaaga aatacactgt
    40801 tattcgccta tgatctttac caacacgcgt tttcttcttc tgccatcgaa ttccccataa
    40861 aagatctgct cccagggacc aaaatccagc ttcccgcggg tcaccgcaac caccacttcg
    40921 cgccccatga gctggcgttt catgtgcgca tcagcgttat cttctccggt gcgattgtgt
    40981 tggtacccgc tgaccggatc atggggagcc agcttctcca accaggtgtc gtaatcgtgt
    41041 aaaagcccat cttcatcgtc gttaatatat acactggcag tgatatgcat ggcattcacc
    41101 aggcacagac cttcctggat gccactctcg cgcaggcagg cttctacctg aggagtgata
    41161 ttttgatagg ctctgcgagc aggaacatca aaccataatt ctttgcgata gtttttcatg
    41221 tggaactcct gatatggtga ttacagaaaa cttattggtt atcagtaaaa aaagaaccgt
    41281 tgataaaatg ctaccacaga gaagtcaaaa tgaaagttca agctgaaaaa agggaatgga
    41341 tgcagtacac ttaataaggt tgagaaagaa tggataacga agctgtattt cgggtgcgca
    41401 tgcccgaggt agatatcaga ccagggttgg aagcaatatt cacccaagcc agagagctgg
    41461 cagagggaga aaccaccctg gcagatggct cccatttacg tcgtgtcgtg atcgtgtctc
    41521 ccgggcgctt attgttgatc aaagattcct tccctcccga tactatgccg ctggaattgc
    41581 gactggcatt ggaagagctt cttcccagcg atcgtgctct taagattgca gtcattgctt
    41641 atacagacct ggacgcgctg cgcaaggata tccagagagc catccctttt tttgaccatt
    41701 tactgggttt tgcatactta gggcatgccg tatggatttt cgaagggcat atatcggtat
    41761 tggagatggc ttgcctgggt gccgactacg tgctggttga tcaacgcatg ttaccgttct
    41821 tagatccaga ttgggagcag atcgtaaggt taagatgcgg agtagcgaac ataagagttc
    41881 tatcgatccc ggaggaataa aaagacccca ccaacgcagg cggggtcttt gttcatacgg
    41941 attacgatct atggataatt tttattctca aacagattgt aggtgatgta cttttcatca
    42001 aggttgtaca ttttgatggc gatcttttct ttgtagtaaa gctgcgatgg gatcgcaaag
    42061 gttgcttgaa atgatgcgcc atccgctgaa ttgaacccgg caacgtaata ccaggttttt
    42121 gcagcggaat tcccatcttt catatacacg ccgaacatat ccccggcggg gaagtacttc
    42181 gtttcaactg ttacagattc acctttcacc acgctgagga tattaaaggt tgggaaatca
    42241 ctgggtgcgt aggttgtggt agatgaagaa ctgcttgtac tgtagttggc agtatacgac
    42301 gttgtattat agaaaaagtt atagctgtag taagggctgg ttggactttc gaggcgaata
    42361 gcgacaatat cctctccctt cagttcgtcc gggatataga atttcgcttt gaaagttccg
    42421 ccggagccgg tccaaatctt ggtgaccagg atgccattta ttcctaatgt gccgttatag
    42481 cccatgtaaa cgtagtacag ttcattggcg ggaaacagat tcgtcctgat ggtgaccgac
    42541 tcatccacca caacactcat gatatcaaag gtggggtaag tggctgcctg gacaggaacg
    42601 catactatca agataagcaa taccgcgata agcaaagcaa atttcttttt catgatctcc
    42661 tccgtgatcg agcattcgag ggcaaaaaac cctgttgtcc aatgaaagtg ccgtagcaag
    42721 aaaaacacat ttctatgctt tcgttttcat tatagccaat tttttaaggt tggcaatatc
    42781 atgatttata ttgcaactat catgccagat tgtgcagaag cctttctttt acatacgtgt
    42841 tgagctctgc tatgctgaga tctttgatgc ccaatttttc gaccgtccga ccggtgcgat
    42901 agtaatctgt tttatggatg atgcatgcca ggcggatcat gctttcgatg ccgcgcaccg
    42961 aaattccata ttgtttgccc agcgatgcaa tgggaaccag actcatgggg acgtcttcga
    43021 atatatagcg gtgcgacaaa gtggaagggg ctttgatgcc ataatacccg ctctgattat
    43081 ggatagcttc gtgcaggtct ttaccctgaa cgttgtatgc cagctccaac cattcctggg
    43141 cggtgcgggc gcggatgccg actgccgatg ccaccgtaac acgttcgcga tccagggctt
    43201 ctaaaacata gccaactgac ggcgtgactc catcgatgta gaattcaaaa tcaccgtgcg
    43261 ttgattcaat gcgaccggca ttcagaattg taagagcagg gtggaagatg gcgcccatat
    43321 tgttcaatcc ggtgtgcaga acatcggtac catggatgaa ctccggatac acgtcttcga
    43381 tagcttgcaa taccagatcg gttcgattag cgggcaatgc tgccaccgga acggcttctt
    43441 tgatacggaa aatgcgtgcc tgtgccggtc catcggaacg gctggcataa ataaaggttt
    43501 cggtttcagc gacgatcgca tttgccgtgc atccttcgcg tcgcatggct gtgtcgaact
    43561 cgatggcgcc aaatgtgcgt cccgggttga gcaggatgat ctgcccatcc ttcacgtagg
    43621 gtgctatcgc cctggcgaca tcggcatgac ccgaagatgg gattaccacc ataatcacct
    43681 cagcagattc caatgcttct gcaatatcgg aggtggcttt cttgatcacg gcaaaacctt
    43741 gcggaccgcc cggatagctt tccaattcaa tacccttgcg tttggcgatc acttcaatgt
    43801 gactgaaggt acggttgaaa agggtgacat ccttcccttt caatgccaga tgcgcagcca
    43861 tagctttacc accattgcct gcaccgacaa ctgtgtaatt tttaggatag gtcattgtta
    43921 cgtcctcctt tttattgcaa aaattgggca agccgctgtt cttcagattg tggattgccg
    43981 ttgtcatcaa caatttcgca ggcaccgttg atgatggtgg tttttatttt tcccctgcca
    44041 aatttattat tgagcaattg cggtgcatcc agaattcctt ttgtaaccgc tttggctaag
    44101 actgctgcat ctgttaatgg gtcggcagcg ctttctgcac caatattgtg aatagcctgc
    44161 agcgtggtct gcgcttcatg caccaggtat tctttacgct tttggatggc tggatcttcc
    44221 agcatatctg gttgcccctg taatgcgttt tcgatcaccc gttgcgccat taaactggct
    44281 tcgatcacat cttgggcagt tgcggcatga tgggcttccg tgtgaccaac gatatgcaca
    44341 atatctggtt tcaatgccat ttgcagatag atgctggctg ccaggtgcgc ccgggcggcg
    44401 ttgatctcaa gcggataact gagcaaaccg gtgcgggttt gtttccataa atgaaaacca
    44461 tcgctctcaa gggtagcgca caactccagg cacgccagca tttttgccag gtccatagac
    44521 tcagacgttc cagttgggct gttgaacatc atttgtgcaa tgtaatcgtg cacgccaagg
    44581 tgtttggcat tataagctga gaggtaggca gctaccacat aaataacgtc cggtccatcg
    44641 cgcattcccc aatgatgagg ctcgttcagc tccactggca cattatgctc actgtaccag
    44701 cgaatgagat tttgatgttc cctgatgccg gcttccagat cgaatggtcc gcgaccgtcc
    44761 atctggttat accaaaacag gggaaccgcc gcccaacaaa tgtggatggt ctcacaatac
    44821 atagatgcca accggatcag atcgttggtg ccggaatatg tgcgcatgag ggggtaattc
    44881 cctgtgcgtg aagccttata caagcgggca tagtcttcag gactgcgtac gggaacgccg
    44941 ccagcgccgg tggcgcgcgg attttgcttg ccaggatgaa aaaagttttc ctgagcgtct
    45001 tgatcaacgc cgagggagat cacgtctagc gctttcgcgt cagctatttg ttcaacgcct
    45061 tccacggtcg ctctcacggt gggttgaccg tagtgatgcc gtagcaatgg aaagggtctt
    45121 ttccaaagaa tacgctcaac ggcactttgt gggtagatga gggcgtcgct ttcggtatgc
    45181 ggatcgccgc gcagaaaagc aacgatctcc tcccgacttt gggtgccatc aaagaccgca
    45241 ttaaaaaacc ctaatttttt ggctttttcc gctacgggtg ctgtgccacc aaaggcgaat
    45301 tggattccct gactgtgcag atcgtctgcc gcttcggcaa actcggctaa gagccgttcg
    45361 ccggtttcag gcgtcaggcg atacgaaaca gcgatcaggt ccgctttttc ttcccgtgca
    45421 gtttggagga tcttttctga gggaacggcg ggaccgagaa aaatggtttt ccatccgcat
    45481 tgctctgcca aacgcaggaa gttataaacc cctgccacat gcacacattc gccaagagca
    45541 cctgctacta ctgttttcat ttttttgctt gccacgctat ttttgttttc atgggtggta
    45601 aaaatctgat atagaatttt cagacaggaa acaactttat taccgagaaa agtgatagga
    45661 tgggttaagt taccgataaa ttactggttc aatgatacca aaaaaaggag aattatgggt
    45721 tcaataaaag tattgctggc tggtgaatca tgggtttcta attccacaca ttacaagggt
    45781 tgggattact tctcaagcac ggtgtatgaa acgggcgaag ggtatattcg aagcgcattc
    45841 gtagaagcgg gtatcgacta tacccacctt cccggtcact tggctgctga aaaattcccg
    45901 ctttccgtgc gagaacttga gaattacgac gttatcattt tgagcgatct gggagccaat
    45961 accttattgc tgcatcctga tacctggttg catggtaaat cggtccccaa ccgcttgcgg
    46021 ttgctggctg attgggttag tgaaggcggg gcgttgatca tgtgcggcgg atattattcc
    46081 ttcgcgggca tctatggggc agcgaaatat taccgttctc ccatcgagga gatacttccg
    46141 gtcaagatct atccattcga tgatcgcgtg gaatgtcctg aagggctgac tgccgaagtg
    46201 ttgatgccct cacatcctct ggtgaacggt atggagggtg attggccatt gctgcttggt
    46261 ttgaacgaaa tggaggtcag agcagaggct gatttgatcg tgcgggcggg aaagtacccg
    46321 ttgctggcgg catgggatta tggaaaggga cgcagcatga tctgggcgtc agatatcggt
    46381 ccgcactggt gcacggtgga attcgccgag tggcagggct acacaacgct ctggaaaaat
    46441 gcagcccatt ggttgagcaa gaaatgaatt aataaaaaat caccttcttt caaaaaggtg
    46501 attttttata tcttccatca tgattttcat aagaaatcat gtacaatgaa ttaattattg
    46561 gtacttttat tagcagagag gtgtgatgga tggtattttt gtctgttctc atttcattgc
    46621 agatcagcgt cttcacaaac tgccttttcc tacggatttg gcattttacc gggcagaact
    46681 ttctccacaa attatcccaa ggcaccctga ttgcggtttc aagatagttt gatcgtcgtt
    46741 gctgttcgag tgatgaatgc aatgatcacg cgcttgttat caattcatag gtttcgtatg
    46801 aaatttaagg aagtaaaaaa tgaaagaaag atatttgttc taccaactta atcgctgcac
    46861 gggctgtcaa ttgtgcatca tggcatgcgc tttggctcgg agaggggtgt gcggtgagaa
    46921 tgaatcgctg atcaagatcc tgacacaccc acagttcgga acgtctcagc cgctcgttga
    46981 tcaatcctgt gagtgggaag aatgtgacgc gcgctgtgtt caggtgtgcg ctcccggggt
    47041 tttgcatctg gcagagaaaa aggaatgggg tagtttaatg acaaagcagg gttggacccc
    47101 cgtgcctgtt ttggtaatgg agaaagaaaa atgagcggag gatattgcaa ccaagttgcc
    47161 tggattgatt tatcttcgaa gtcggtttac acgaaacctc tggatggtga aatcaaggag
    47221 cgctacattg gcggtcgcgg attcatcacg cgctggttga tggacatggt gccgccttct
    47281 gtacagccgt ttgacccggg taatgcatta atttttgcaa caggaccgtt gaccggaacg
    47341 aatgttccca cctcgtcccg catagaaatc ggggcggtag ctcctgaaac aggtattttt
    47401 tcaagcggaa gtgcaggcgg cagctttggc acaaagttga aacgcgccgg gtttgacgcg
    47461 ctggttatca caggcaggtc tgaaaaacca gtttatataa tcgttagaga caatcatgtt
    47521 gaaataaaag acgccagacg ctattgggga agagacgtgt tcgagacaga tcgagcgatc
    47581 aaagccgatc atcgggattc gcgtctatct atggcttgca ttggacaggc tggcgaaaat
    47641 gaagttctca tcactaccat tatgatcgat cgtgtgcgcg cagccggacg cgggggattg
    47701 ggcgccgtga tggggaacaa acgattaaaa gcgatcgcgg tctatggtag cggaagtgtt
    47761 cccatccatg ctcctgaacc attttggaac gcatgcgaga ttattcgcga ggcggcggtg
    47821 aagcaatttt tcgtgaagcg ttgggaaaat ggtacgtatg gaaatttgac acgctaccag
    47881 ggtgtaggcg cattgaccag ctacaactct cgcaagacaa cattcgaata tatcgatcga
    47941 ataagcgcga cccactacaa cgaaaattat cgcgttggca ggcgtgcatg cgtaagctgc
    48001 ccaatgccgt gctggacaac atttgtgatc caatctggag agtttaaagg gttcttcgga
    48061 gaagagacga ctgccaccac ttttaaagaa atgggcgcac gctgtggttt gcatgagatg
    48121 gacgccattt tgaaagcaca tagtgacttg aaccgatatg gattagatac gatatccacc
    48181 ccggcggtca ttgcctttgc gatggaatgt tacgaagaag gggtcatcac aaaggaagat
    48241 accggaggtt tggaattaac ctggggaaac cgacatgctg ttattccact gattgagcaa
    48301 attgccggga acaaaggatt tggcgctcaa ttggcaagag gagtgcgcat ttgtgcgcaa
    48361 acatggggag aaaaaacaat gaagtacgcc ctgcatgtaa aaggtctgga gaccgttggt
    48421 acagatccac gtgggcaacc atcctgggga ttgggttatg ccacttcaag ccgcggagcc
    48481 tgccacatgc gcgcttatgc gaattatgaa tctggagtca ataacgcacc cggtggcatg
    48541 gacgaaaaga ccatgatccg catttcaggc acgacagaga tcggaaaacg cttcagtacc
    48601 actggaaagg ggaaagccgg agcattcctg gaggatatga atgcttttgg agattcgttg
    48661 ggaatttgta aactcatgtc aagggctgag ttgggtttgc cagaatcctt ggtggatgtg
    48721 ttgaattatg ctactggtgc agattatgac acagaagggt tgtatcgggt tggggagcgc
    48781 atttctaacc ttgaaaggat agcaaatcta caacgcggga tcactcctgc ggatgatact
    48841 ttgccggaac gttatttgca tgaacctgtg ccagaaggac cggcggcggg gcaggtgtgt
    48901 gatctggaac ccatgctgaa cgaatattat gaaacgcgcg attgggatcg tgcggatgga
    48961 tatccatcca cacacaagtt gaatgaattg aaattggatc gcatgggaaa agatattgaa
    49021 taacatcaat ggctgtcctc tttgagagca aacgaagacg aaaatgcagc aaatcagaat
    49081 ggtgaacttt ggtgcatttt tcatccgtca agaaccaact acagtacaat atggtacaaa
    49141 tgctcatcat agtgaaatta tgttaatcat tgcagaattt taaaattggg catggcacac
    49201 aatcagatac tttccaatgc tggatcaagc gaacagtaaa aaggattgcg atgaacagtt
    49261 atgagatcat gaaacgcaat attcagtttg actgccctga aagaattggg ttgcggttca
    49321 acagcttagg ggttagcgat gtgttccgaa tttttgttca acctccccag cgctgccgcc
    49381 ctgcagacgc gcagaatgtg cgcatggata aaaagatccg tgcaacccca ggtcaggcag
    49441 atgaatgggg ttgtatatgg gaatcgctgg aagagggtag tggggatatg gggcaggtgc
    49501 tccatccgcc catgaaaagt cttgatgaag tggattcgta tcccttccct gatccgcacg
    49561 atccgtgcca ttttgatgga ttagaagaag ccctacagct ttcgaatgac aagtttgttc
    49621 aactgaacag ccctttttgc ctgtttgagc gtatgcattt cctgtatggt tttagcgaaa
    49681 ccctgcgcga tctggctctt gaacctcaga agatggaatc gttcatcgat aaagtgatcg
    49741 attaccagat agggattgca gaaatggcag gaaagcttgg gaaaggtcgc atccactgtt
    49801 ttgacactac cgatgattgg ggcacgcaga ccaatatgtt catcagcccc aaaatgtggc
    49861 gtgccctctt caaaccccgc tataaaaagt tgatcgatgc cattcatgcc aatggcatgg
    49921 tcatccgatt ccattcagat gggcgcatca acgcgatttt ccccgatttg atcgaactgg
    49981 gtgttgatat tctcaacttt caccaacccc tgttggtggg tattgatgag gtggctggac
    50041 aattgaccgg aaaagtttgc tttgaggttt cagcagatgt tcaacaaacc cttccaactt
    50101 gcgatgaaca gaaaattaaa aatgagataa aagaaattgt tcaaaagtgg tgtactccca
    50161 aggggggatt gatcggcatt gaatatcgct atgggaaagt gcttggtatt tcagagcagg
    50221 cattacgctg ggaattggaa gcgttccagc agtatggtaa tttcaaatag agaggttgat
    50281 aatgattaac ggtatggaac tactagaaaa agaaggacca gcatatgaac ggcgcagcca
    50341 gcgttttcaa agctggtacg agagcgttta taaatggcat ccgaattttg tttttaagcc
    50401 gaatgcgaaa atcaattgga gcgcgatcac gaaaccaatg tcagaatgga aagtagggct
    50461 ggtttcatca agcggaaccc atttgcgttc gcagattcca ttcgatatcg tcagcgatga
    50521 cggagattgg tcatatcgag aaatccccgt ggataccgat cctaaggacc tcatggtctc
    50581 tgacgcgcat tatgaccaca gtgatgctga ttcagatgtg aattgtatgt tccccattat
    50641 caccttgcgg gaactggtca aagaaaaggt tattggttca ctcccaacca ggttctacgg
    50701 atttatgggt tttgtgcctg atcccactga actgatagcc cataatgccc ccgaagtggc
    50761 gcaaaaactg gcggatgaaa aagtggatgt cgtgatactg acccccggtt gcgcggtgtg
    50821 tcatcaaacc ctgggcatca ttcaaaatat tatcgaagga gcgggcattg caacgatagc
    50881 caccacgcta aaaccagaac tgaccgaaca gatccatgtg cccagagcag cttatttgaa
    50941 atatccgtat ggctattccg caggaccggc gaacgatctg gaaatgcaaa aaaagatcat
    51001 caaagaagtg ttggaactgg tgcctgcaat caaggaacca ggcacaattg tgaaaatgcc
    51061 ctatcgctgg catggacgat ttgaatagga taaggagaag gcaatggcag tagacaaaag
    51121 aaccgatcag ttgatggctc atgtagaagc aatgcttgag atcatcgatg acatgaatca
    51181 ggatatgcaa tccaggatcg aggaagagaa tgccagtgat gctccgaacg attcccgtat
    51241 caatttctac acatcacagc aaaggagatt ggttaagtta accgatctgc ttgaagaaga
    51301 tattttagaa gtgttgatca gcattcgcaa catctcaggt ccgatcgaat attttgagaa
    51361 ttaagcagct ttgctgcacg gacgatttcc ttacccttag aaggtcgata aaaataccgc
    51421 tcggttctga cgagcggtat tttttatgct actccgagat agcttttttg aaccatttcg
    51481 ctttcgcgaa gctcttttga tgttccactc aggctcacca accctgtttg taggacatat
    51541 ccgcgatctg ccaccgaaag tgccagtttc gtattttgtt ctaccaacag aaccgtagtg
    51601 ccctgcgcat tgatctcttt tacgttctca aaaatgcttt ccagcaagat cggagccaac
    51661 cccatggagg gttcatccag gagcagcaat ttcggttttg ccatcaatgc gcgtgcaatg
    51721 gctagcattt gttgttctcc acccgatagg gtaccagcag attgcttcga acgttctttc
    51781 aaccttggga acaactggaa tgcacgttcg atatcttctt ggatggcgct ccggtcgtga
    51841 cgggtaaaag ctcccatttc cagattctcc aaagtgctta actttgaaaa tacatgcctg
    51901 ccctctggaa ccatagaaat ccctttttca acgatgtgat ggggttctag aagggtggtg
    51961 tcttctccat tgaaaaagat tgatccctct tgtggatgta gaagaccggt gatggatttc
    52021 aaggtagtgg tttttcctgc tccgttcgaa ccgatcaggc aaacgatctc accctggttt
    52081 acctcaaggg taactccttt aagagcgcaa atatttccat agtaggcttt gattgaattg
    52141 acttgaagta agggagccat tgtgcattcc ttttattggt agagatcgtt cttttttccg
    52201 aggtaagcct caataacagc gggatcattt tggatttctg ccggttttcc ttcggcgatc
    52261 ttcgatccaa aatcgagcac aatgatcttt tcgctgatct gcatgaccag tttgatatca
    52321 tgttcgatga ggatgatggt gatgtccaat tcgtcccgca aacgcctgat gagtctgatc
    52381 atctcctgtg tttcagccgg gttcattccc gctgtgggtt catcgagcag caaaactttc
    52441 ggattgttgg cgatcgctct ggcgatctcc aattttcgct gtgctccata cgacaggttg
    52501 catgccaatt ggtctcccca acctgtaaga ccaacaaagt tcaagatctt cttcgctttg
    52561 tcgatcgcct ctttttcttc tgagcgatag cgtttggttg agatcaacga atcgccgaaa
    52621 gaactgcgca gtcgaatgtg ctcaccagcc aagatgtttt caagcgcggt aagctcttta
    52681 tacaaacgaa tattctggta agtccttgaa atcccttcct ctgcgatttt atgagggggc
    52741 agaccggtta tctctttatc cagaaacaca atattgccgt actcaggttt gtaatagcct
    52801 gtgatgcaat taaagaatgt ggtcttgcca gccccgttag gaccgataac gctggaaatg
    52861 gatgcttgct ggatttcaac gttgagatcg tggatggctt caagaccacc aaagcgtttg
    52921 gttatattgg atgaagataa aatattcata attgcacctc tttttcattt tcgggcgaac
    52981 tgcaagggtt tttctcaatt tgtggagcct tgcggggaag aaaaccctgt ggacgcaaga
    53041 tcatcatgat gatcaataac aaaccgaaga tgatcatacg gtaatcggaa aaaccacgca
    53101 gaatgtcggg caatgcaatc agcgccattg cgccaagaat aacacccagg tggtttccca
    53161 gccctcccag aatgatcaca cataaaatgt tgatcgatac ggttacgttg aaattgtcgg
    53221 ggaaaatgga accttgccat gaagagaaaa tcgcgcctcc cagcccgccg atgaaggcac
    53281 cgatggcaaa agccaacatt ttatatttgg atgtgttgat tccggacatt tgtgcaacat
    53341 cttcatcttc tctcatggcg agccaggcgc gcccggttct ggaattgatg acgcgattga
    53401 gaaggaaaag caaggtcgca atcaacaaaa agataaaata atagaagtat gtgctgttac
    53461 tgaagttgat cccgaagatg accggtttgt cgatttgaat aattccttgg ctaccgttgg
    53521 tgatatcggt gaggttcatc aacaacatgc gcacaatttc gccaaagccg agggtcacga
    53581 tgcccaggta atccccgcgc agcttgagaa ccggaatgca caccagagca ccaacgatgg
    53641 cggcaagcgc cccggcaatt aggatgacaa gcaaaaacgg cagatgaatg ttgaaatgcg
    53701 gtgacgcaag aaaagcatag gtgtatgcac cgatggcgta gaaagcggca aaacccagat
    53761 cgaacattcc tgccagccca acatgaacca gcaaacctaa tcccaaaatg atatataagc
    53821 cggcgtagcc gatgatgcgg atccacaggt tcttttgagg aaaactcagc agagggatgg
    53881 aaagaacgat gatcgatacc aaagcaggga ggatggcacg gaaaactttc tggtcaagtt
    53941 tggcaattgt tttttcttta tttttcatga tttcacctga cctatagttt attttttcca
    54001 ccgctaccgc ccaggatccc ggtcggtttg acgatcaaca tgataatcaa aatggcaaaa
    54061 gcaaccgtgt ctttatatac agccggcaag aattgaatgc tgatcacttc tgccaatccc
    54121 aataaatacc ccccaatcat tgctccgggg atgtttccga tgccgccaac gaccgcggct
    54181 gtgaaagctt tgatgccagg tgtgaatccc atcgttggac ggatttgcag ataaaagatg
    54241 ccgaccatca cgccggcaat ccctgccagg gcagaaccga ttaagaaggt gttcgaaatg
    54301 atacggttta cattaatacc catgagcgcg gcggtatctt tgtcttctga tacagcacgc
    54361 atagccttgc cgagttttgt aaaacggacc agtagataga gcaagatcat tattgcgatt
    54421 gcaatggtca tgatcaggat cctgctgtaa gagatcacca cttccccgat ccgaatacca
    54481 cctgtcccta aaatgcccgg gtatgtctta accatggctc ccccgaagat ttgggttaca
    54541 ttgacaatgg cgattctcca gctttctgga aagaggtttg tatcaccaaa gtggatggaa
    54601 ctgaccatgg agccgaatcg ggggatgagg cggacaaact cttgaagaaa aaatgatacc
    54661 ccaatggcgc tgatcagtgt tgccaggcgg ctggcttttc ggagtgggcg atatgccaat
    54721 ctttccactg tggcaccggt gagagaggcg ccgattgcac cggctaaaaa tgtaaaaatg
    54781 attgctagaa taggattgct ttctgtataa ccgatctttg ataattgcgt gattgtaaaa
    54841 cagccaaagt atgcaccgac cataaaaata tcaccatgag cgaaattgat catggagagt
    54901 acaccgtata ccattgtgta acccaatgca atgagcgcat agaaactacc cacggtcatt
    54961 ccgttgataa gctgttctat taatttctgc ataacgcgcc ctccgaaaag agatgaaaag
    55021 gggggattgc tcccccctta aggtttattg accgaagtcg tagactttca cgggaacaag
    55081 ttcgttacct tgtgcctgga atacagtgat cgagacgact ttcagatcgc cgttctcatc
    55141 aaattcgagg tgaccagtaa tgccatcata gggcgttgca cgaacggcat cggcaagggc
    55201 tttcttgggg atgaccaggt ttccgtcagc atccaccgtg gcgactgctt tggctgcatt
    55261 caggatgatt gtggctgcat cgtaggatcc gggcccgtaa gcgacggggt cgccaaattg
    55321 cccgctgaat ttttccagga attcatcgta accggttgca ccaccaaccg caccaaaggt
    55381 catataagca ccttctgctg caccaccaga agcttctacg aaggaaggaa cggatttgat
    55441 gccgtcaggt ccgaagaaga ttccttcaaa gccggctgtg cgcagttgat tgacaagcag
    55501 cgcgccttca gcgtccatgc caccccagta gattgcggct ggatcagctt cgagaatgat
    55561 tgatacgaca gcagagaaat cctggtcgcc gcgtgtgata ccttcaaaac cggtaacagt
    55621 gccgccggca gcttcgaatt tggatttaac tgcttcggca atgccctgtc cgtaaatact
    55681 ctgatcatgg acgattccga gggtggttgc tcccagttcg ttgaagaggt aatcgacggt
    55741 gacttctgct tgcaggtcat cattggcgac gacgcggaaa atgttttgat tgccagaggc
    55801 agtcactgca atagctgtgg atgaaggtgt gatcatgaga acgtgattct cagcgtagat
    55861 atctatggct gggatggcag tgccggaaca cataggaccg acaacaccaa ccagatacgg
    55921 attggctgag aattgctccg caacagttac gcccggggcg ccttcacatt ggtcatcgcc
    55981 accttcgact acaacctccc atccattcag atcaccaaag gtctgaatgg cgactgtagc
    56041 gccattgagc atatcttgac cataggctgc ataaccagcg gaaagagcag aagaaagacc
    56101 gagcactact ttttctccgg ctgggacaac gatatagccc cattcgtctt ctgcaacgct
    56161 ttcacctgca tcagcagcgg gagcttcttc agcaggggct gtaccaccgc acgctgcgaa
    56221 taacagcatg gcaacggtca gcaatgaaac aaaaatgaaa cgtttagaca taaatctcct
    56281 ccaaatggca atagtatttt gatgcatgga caaaatgacg ggttcttctt tcgtggcaca
    56341 tcacctcctt tgtgatgagt tttccataat tgcagggttg tcaataaatc ctttcacagt
    56401 atcaagggtc gatgaagtgg caaagatgct gcagttcgag cgcctgtaat taattggcgc
    56461 gattatccca caattaacga caaatgtcaa ctattcaata tgatgaatgt catatttgta
    56521 tgacaattga cgagaggggg gggatggctg ttgcagaaga gctaagattt tgtttggatg
    56581 aacgattggt agtgacggat ggtgttttga gatggttcta acccatattc ttttgccagt
    56641 tgttgtttgc actgttcaaa cacgcgaatg atggcagccg tattccctaa agcatgataa
    56701 atgctcatcg cgttgcaata tgcttcttcg tttgaggggt cattttggat aatctgtctg
    56761 ttgatctgca atgctttttc caggtctttc tggttcatgt agcaggtgta caacttaaga
    56821 agagcgttca tatagatatc gaaatagtgc tgtctttcta tgccgaacca ggtagcgtct
    56881 acgtctgaca aaaatgggcc cttgtacaat tccacagccg attcgagcaa ttggatagct
    56941 ttgtgcgcat ctttctcgtt ctccgcttgg gctatgtact gttcgaacga taacacatca
    57001 tattcgaagt cgacagcgtt gttaaaatga taataattat cggtgaagag gacaacattg
    57061 gacccgattg ctctgcgcat gcgatagatc gcgttcttga acctcctttt taactcttcc
    57121 atagatgaat cgggccaaag aatgagccca acttcttctt tggtcatgcc ttcaggattg
    57181 gagaggaaaa gaaaaaagag atcacgagat gtttgggtca tccaatcaga ggttgagaga
    57241 agcttgttgt tcagatacac ttcggttttt ccgaaagcgc ggatgaccaa tttgggttcg
    57301 gcaaagggaa ctacagaagc gtgtctgcgg attttccggc gaatgttctg tattttcttt
    57361 tgataattcg cattctggtc gaggatcagt tcaatgtact cggcgatccc tgcctgccct
    57421 gagagcagtt tgagatcgtt tctgagatca aagcaggtag agagaatgtg ggtgttggaa
    57481 gatggttgat tgagattgtg gatgatttcc tggaagatag cggcggactc ttcggtcatt
    57541 ttttgttttg ccaacacaac tgccagataa agcttgatgg taaatatcgt atcgggttgc
    57601 cccatgcttt tgaagaaatc aagcgatgcg agaagcatgg ttaaagcggc tgaaaggtct
    57661 ccgaaataat acgtgaggat cgctttttcg accttgcact gttcgatctc caattgcgag
    57721 cctgaccttt tggcttgatt gaatgccaat tcaagaaggg tggtggcttg tttgaattgc
    57781 ttttgcaaga tcttgattct cgtttcagcc aggcgtaaat acaataccag atattggtct
    57841 tcaatatctt gtgcaatttt cattgcatcg gaatatgctt cgagcgattc ttgatgggca
    57901 tccaggtttc ggtagagatc accaatactg gcaagcgagt agccctccat gcgccggtta
    57961 ctactcatct tggcataatc catcgctttt tccaggttca tgaagctatt ttcaaaatcg
    58021 cccctggcat gctgtaatac tcccaggttg ttgtatagat tcgattgcca gatcgaatca
    58081 ccaatttgct tccagaactg taatgattga ttgtatgctt tttcggcttc ttcgaagttt
    58141 ccgagcgttt cgaagataat tccaagttct gcctgaattc tggcacaatc ttctggcgaa
    58201 ttcgattttt gccataacgc cagtgacctt tccatccagg tcagcgcttc atccaggatc
    58261 ccttcctggt agtaagtcat tcccaccgca cgcatcgctt cagctttgat cgaattcgat
    58321 ttttgtattt cttcggataa gcggagtgct tgttgagcat cttgatgaga ttcctggtat
    58381 ttaccaagca agcgcagagt cgttgaacgc cgtaccagat tttcggcaat ggagaccttg
    58441 tcatctccct ttttctgttg aagctcgatc accgcgttca acaggtctaa cccaaccggc
    58501 aggtttcctt tgtttacttc gatgctggct ttgatggaca tcagttttgg atttgaacgt
    58561 aatttttcct cagggatatc tgaaaaccaa ttttccagcc gtttgatttt tccctttgag
    58621 atgaatgaag aaccgataga ttcaatgagg tggataatag tgtcaatatc gcccagcttc
    58681 aagtaaatga cgaaggcgtg ttcccaatct tgcgattgcg tatggtattc ggctaatttt
    58741 cttagaatag accgggtttc ttctggatac atggtttcca tacggtgctg taagaaatcg
    58801 cgaaacaggt gatggtaacg cagccagaat ccctcatcgc caatgcgcac cacaaacaaa
    58861 ttgttgaaaa caatgtcttc catcgacgat tgccagttac ctgcaacctg tagcgcttca
    58921 ccgatgatca gttgacacat ttcaggattg aactcttcca acaatgatga tctcagcaaa
    58981 aattgttgga tgtctggact ttgctggtcc aatacctgct gagccagata ctcgtataac
    59041 ccgattccag atgttcggct gatctttgcc tgatcaccga tccccttgtt gcctacctgg
    59101 gctgtcagca acaaaccggt gatccaaccc tcactctggc gggcataggc ttcggcttcc
    59161 tccttgctga tttccacatt gtggttcttt tcgaagagtt ttttgatttc atcaggttga
    59221 aagaccaatt cttccaggct caatccaccc acctgtgaac gagctaccaa caggggcaga
    59281 tcgggcagag taagcaacgt gcgcgatgtg accaccagat gaaaattctc ccccactttt
    59341 tggaggaaac cgctcaaaaa ctcatcgagc tccttgccct ggttcaccag gtgataatca
    59401 tcaagcacca ggatgaaatg ctctgtgatg ttttcaaaaa tatcgttggt tatggttgtg
    59461 acaatggaat caatatccaa ttgatcttcg ctcaaggttt ccatcagggc attcgagcgg
    59521 accccgaagg aggggaaccg ttgttggata gcgctaataa aatatgtcaa gaagcgggtg
    59581 aggttttggt cgagcgggtc catggctaac caggcgatcg gccaatcata cgaagtagca
    59641 taatcgatca tcaaagatgt ttttccgtaa ccagccggag ctgcgatgat gatcaattta
    59701 aaatccagca ggtcatccaa caacgttaat aagcgggggc gcgataataa ttctttcctg
    59761 cgttgaggaa cgatgatctt ggtctgagag atgggtgaaa accatgccat tgctgtttat
    59821 tgtatcagtg agtaaaagtg tagaacatag gacgattgtt gcaattcatt ccatccacct
    59881 accaaccggt gacctatatg gggcgcgaag gaacgagcgc tgtatgatta attgtatagc
    59941 aggtttcgaa ggcgatttga gtataatagt tttgagcacc atactctttt tgaaatgaaa
    60001 aacgatgaga attatccata tttttggagc acgccctcaa tttatcaaag ttgctatgct
    60061 tgccagagcc tggcaaggga attgcgaaca gtttttcctg cataccggac aacattatga
    60121 tgttgatctc tctgatgcgt tgatcgcaga tcttgatatg ccgacccccg atctaaatct
    60181 gggggttgga agtgcttctc ctgcaataca gacagcacaa atgatggtgg ggatcgaaga
    60241 attcatgatt cacaaaaatc ccgattgtgt ctttgtgtat ggtgatacga acagcacgtt
    60301 ggcagcagct ctggtggcgt caaagatgaa aacaacgctg gcacacgttg aagccggtct
    60361 gcgctcattc aatcgatcca tgcccgagga gatcaatcgc attgtcacgg atcacctgtc
    60421 tgactacctt ttttgtccta cgcgaaccgc actgatcaat cttgaaaatg aagggattcg
    60481 gaaagaggtt gctctgtgcg gggatgtaat ggcagacgct ctcgcctatt atcggcagaa
    60541 tactatggat gaagaaatag ttctgaacaa atatggtctg cggaaaaatg actttatctt
    60601 gcttaccttg catcgccccg gcaatgtcga ttcaaaggag aacctgaaag agatcatggc
    60661 agcggtggaa gaaattggga aaatgattgt cttccccatt cacccgcgca caaagaaaat
    60721 gctgcaaacc ttccggatca aactgcccac caatgtgaaa gcgattgcgc cacttgaata
    60781 tacagaaatg atcatcctcg aacaggcagc tcaatgtgtc atgaccgatt cgggtgggat
    60841 tcaaaaagaa gcttacctga tgggaacccc ttgcatcacg ttgcgcgaag aaacggaatg
    60901 ggttgaaacc gttgcggcgg gctggaatgt cttggtagga gccagccggg aacgaattct
    60961 ctctgcgttc aatgactttc atccaaccgg agagcaaccg gcgctgttcg gagattttca
    61021 tgctgcagag aagatcgtag aacaaacttt ggaatggatg agagggatgg attagccaat
    61081 ggattatttt gttcatacaa gcgctctggt tgaagacggt gctcacgttg gcgaaggcac
    61141 aaagatctgg cataactgcc aggtgagaag cggagcacat attggaaaaa attgcattct
    61201 tggcaagggc gtttttattg acagcggtgt tgtaattggg gatgcggtga agatccagaa
    61261 ttatgtctct atttaccacg gggtgactgt tggaaatggc gttttcattg gtccgcatgt
    61321 atgctttacg aatgatcttt ttcctcgcgc gatcaataaa gatgggtcgc caaaatcgat
    61381 ggaagattgg atcgtatctg aaacacatat tgctgatggt gcgtctttgg gagccaatgc
    61441 gaccattcgc tgtggaatac ggataggaga atgggcattg gtaggcgccg ggagtgtggt
    61501 gacaagagat gttccaccgc aagctctggt gtttggtaat ccagcccgct ttcaaggata
    61561 cgtatgcaaa tgtggtgtga aactggatgt catgaaaacc ggtgaaacga gcacggatta
    61621 tgtttgccca aattgttacg aagaatacaa ttttatgaat aaacaggaag gcatcgaatg
    61681 aatataccca ttgcaaaacc aattcttgga gatgaagaaa agcaggcggt gttgtgtgta
    61741 atggattctg gcatgttagc ccagggttca cgggtggaag cctttgaaga agcgtttgca
    61801 gattattgcg gagtgaaata tgccattgcc gtgtcttcgg gaacgaccgc cctgcatatt
    61861 gccatgctgg cacatgaaat cggaaaagat gatgaggtca tcactacccc cttttcattc
    61921 atcgcaactg ccaattgcct cctgtatgtc ggggcaaaac cagtttttgt ggatattgac
    61981 ccggctactt ataatatcga tcctgccctc gtcgagcgag cgatcacgcc gcgcactcgt
    62041 gccattctgc cggtgcatct gtacggtcag tgcagcaaca tggacgcgat catggatatt
    62101 gcggaaaaac atcatttgat tgtcattgaa gatgcctgcc aaagtcacgg agctgcttat
    62161 aaagggaaga aagcgggttc atttggcacg ggcacttttt ccttttatcc aacgaaaaat
    62221 atgacctgcg gcgagggagg tatgattacc acgagtgatg cggttatcaa tgatcgctgt
    62281 cgggtgatac gcaatcacgg catgcgacgg cgttattatc atgatgaact tggttataac
    62341 ttccgcatga cggatattgg cgcagccatt gggttggaac aattgaataa acttgaacgc
    62401 ttcaacgcga tacggaaaga gaatgcatcg ttcttttctc aaaacttgcg cggagtagtt
    62461 gttccagttg aggtgaaaga atgcagccac gtgtatcatc agtacacaat tcggatcaaa
    62521 aatggaaaaa gagatgcact gcgggaatat ttgaaagaaa gaggtatcgg aaccgaggtg
    62581 tattaccctg tgccaatcca taaacaagct tttattcaaa aaatattccc tgccctgccg
    62641 gttcttgcgc aaagcgaatt agctgccgaa gaagtggttt ccattcccgt acatccctca
    62701 ttaagcgaaa ttgatcgaaa ggcaatcgtt gaatccatca attccttcat ggaggaacgc
    62761 ggtgattaaa actgcggtta ttggggctgg ttctatgggg gcaaatcatg cccgcattta
    62821 tgccggtatg aatgcagcga agttggttgc caccgctgat ctagacaaag atcgatgtgc
    62881 aagcatcgct gaaaaatata atgtttcgac ttataccgat tatcgtgaac tattggaaag
    62941 agaaaaaccc gatctcgttt cgattgttgt gcctaccgcc agtcatatgg ctgtagccat
    63001 ggattgcctg caggcgggcg tgcacgttct gatcgaaaag ccgattactt caacactgga
    63061 tgaagcaggt gcggtcatcg agcgggcaag atccgaaaaa ttgaaattga tgatcggtca
    63121 catcgtgcga ttcaacccgg cggtgcagga gttaaagaaa cacctggatg ccggcgaact
    63181 cgggcgcata ttccaaatcg tatgccatcg agaaggaccg ttccctactc gtattcgtga
    63241 tgtgggagtg gtggtcgatt tggcacccca tgatgttgac atcatgcgct atttatcgca
    63301 atcagaaccc attcgtgtat atgccgaaac cgagcggcgt attcatacag agcatgaaga
    63361 tctcttgctg gcaaccatgc gctttaaaaa tggaataacc tccgcgctca atatcaactg
    63421 gctcaccccc accaagattc gcgagatcaa tgtgctgggt gaaaaaggca tgttccagat
    63481 caacgatctt acgcaagatc tctatttcta tgagaattct ctggctgtga atggcatttg
    63541 ggactcgttg aaaaccatcc agggggtcaa ccccggaaaa atggtgcgat ttgcttttga
    63601 ccgaaaagaa ccgttggaaa tggaattgac ggcttttgtg gaagctgtgc aaaatgacct
    63661 tcccgtccct gtaacaggtg aagatggcta cgaagcttta cggattgcat ctgcccttgt
    63721 gcaatcgggg ttaacccatc aggtgatcga gttgtgatat gagcttacag gaactttcgg
    63781 aaaaaatctc cggcaaaacg gcaacgatag cagtgatagg cttgggatat gtggggctcc
    63841 ccctggctgc ctgtctggct gacgttggat tcatggtgct gggggtggat aggaaacaag
    63901 agcgggttgc agtcattaat cagggtaaaa atccgattgg cggaaaagaa ccaggattgg
    63961 atgaactttt aaaaaaaacg gtatctgccc actatttgca ggcgacaacg gattaccagc
    64021 aattgcggaa ctgcgatgtt gtttttatta atgtggaaac gcccgtagag gaagatcata
    64081 aaccaagtta tcaagcattg cggtcggtgt taaaggcact gggaccggtt ttgaaaaaag
    64141 gagctttggt aatcgttgaa tccaccattt cgccgggaac catgaaaaac atcgttctgc
    64201 ctgatttgga gaagagcgcg gatggaaagc ttggtcatga tttttacctc ggaaattgtc
    64261 cagaacgtgt tatgccagga agattgctgg caaacattca ttccatgcag cgtgtcattg
    64321 gcggtgcaga tgagcaaacc gcgcagacca tggcgttgat ctataaacaa tttgtgaatg
    64381 cagatctgga tctaacagat tgggtaactg ctgaattggt gaaaacagtt gaaaacgctt
    64441 atcgtgatgt acaaatcgct tttgccaatg agattgccat actgtgcgca gaccttggag
    64501 ccgatgtctg gaaagtacgc gaactggtga acaaaacacc ctatcgaaac gtgcataaac
    64561 ccggcgcagg ggtgggaggg cactgcattc ccaaagaccc atggttgttg gcaagtagtt
    64621 tgagcgaaag caaagaactc tctctgatcc ctgctgcccg tgccatcaac gacagcatgc
    64681 cggagcacgt tttcaccttg ttagaaaaag gtctttctac attaggcaaa atgttaaagg
    64741 attcacggat cttaattctc ggttatgcct atctggaaaa cagcgatgac gatcgcaatt
    64801 ctccctctga gctatttgcc aggaggctta tggatggagg gtgctcgttt tccattcatg
    64861 atccatttat tgaacgatac cagggagata tctatcacat ggctaaagac tgtgacgcgc
    64921 tcgtgctcat gactgctcat gaccaatacc gcgacctgga cttaaaaata atgagacaag
    64981 tgatgcgaac cccacttatt atcgatggac gcaatgcatt tgcagatctg accgacctgc
    65041 acggttttga aatcatttgt cttggaaaag gatgaacctg tgatttgggc attgattccc
    65101 gcatttaatg aagaaacagc tctcctctct ctgctggaaa agatcaaaca gggattagaa
    65161 gggtacgagt acaagatcgt agttgtggat gacgggagtt tcgataaaac cccactgctt
    65221 ttagcaaaag ctgcccaaaa atacccttta gaagtgatca cgcatcctat caaccggggc
    65281 ttgggggaga ccgaacggga tggatttgaa tacattgccg ctcatagcaa ccatgacgat
    65341 attattgttc gtgtcgaagg ggataatacc cacgaaccgg tttatatagt gaatctcgtt
    65401 agtcgtctga aagaagggta tgatgttgtc aatacttcgc gctttcaacc gggtggtgga
    65461 caagtagggc ttaatttcta tcgcagtttt atcagccgtt gtgcaaacta ttttatgcag
    65521 gttatgttca acatcaaggg aatacgcgat tattcttgtg gttttcgcgc ataccgggca
    65581 aaggtcatcc aggatgccgt agctatcttt gggaacaact tcatccaatt aaagggtgtc
    65641 gggttcacct ccactctgga aattcttgtg aaattgaaaa tgctcggatg tacttttgct
    65701 gaagttcctt ttgtattacg ctatgatcaa aaacagggaa aaagcaagat ggtcgggagc
    65761 gtgaccacgg tcggatactt attgctggca ctattttata attggccatt tggaggttgg
    65821 aaacacagct atcgcggatt ggcggtgttg tatcaagagg acgttcaaaa agccgccgaa
    65881 cggtatggga taaataaaag ttctggaagg tttctaacca agatcaattc ttaggaggaa
    65941 ggaatgtgcg gtatttgcgg aatactgcat tttgatgcac agcgaacagt agatgccacg
    66001 gttctgacag ccatgtcgga aaagatgatc catcgcggac cggatgattt tgggttatac
    66061 gttgcaggat cgctaggttt ggcacaccgc aggttgagca tccttgatct taccgcggca
    66121 gggcaccaac caatgtcttc cacggatggg cgcttttgga tcgtttttaa cggggaaatc
    66181 tacaattttg tggaattgaa gcgctacctt gttgcaaaag gctattcatt tcgttcacga
    66241 actgataccg aagtgttgct taatcttttt atcgaagagg ggaaagagtc cctgcacaaa
    66301 ctaaacggta tgttcgcatt tgctgtctgg gattctaaag ataaaaaact tttcgttgct
    66361 cgcgacaggc tgggtgtgaa accgctctat tggtatcgcg acgagcacac cttgcttttt
    66421 gcctcagaga tgaaagcgct atttcaattc ccatccctca aggctatgct gaatactgac
    66481 ggtctgcaag attacctttc atttcaattc tgccttggcg aaaagaccat gttcaaaagt
    66541 gttcataaag taccaccggg atggtggatg gagatcgcac agaatggtga cacaaaatct
    66601 gggtgctatt gggatctgga gttcaaagtg gaccccgatc attcagaggg ctatttcctg
    66661 caaactttag gcgatcttct gcaagattca atcgcgttgc ggcttcgctc tgatgtgcca
    66721 cttggctccc acctcagtgg aggactggat tcatcggtgg ttgcatctct ggctgccagc
    66781 aatctccagc aacctattca taccttcagc ggcggcttca aagaagatgc aatctttgac
    66841 gaaacgaaat atgcgcgcat cgttgcggaa caaaaaaata ctatccatca tgaggtgttt
    66901 ccgacagcgc aggactttat tgatttcctg ccatccatca tttatcacat ggatgagccg
    66961 gctgcaggac cgggcatctt tccacaactc atggtctcgc agatcgccag ggaacatgta
    67021 aaagtagttc ttggcgggca gggtggggat gagttattcg ccggatacgc acggtatttg
    67081 atcgcatatt tggaagaatg cattcgtggt ggaatcgaag cgacccagga agatcccggg
    67141 tacattgtta atttccaaac gatcttgcct aatttacccc agctacaagg ttatcaagcg
    67201 ctcatgcgcc atttctggaa agatggattg ttcgaaccgc aagaagcgcg atacttccga
    67261 ctgattgata ggggtgatag cctgcgcacc ttttttcata cagatcttat tggtcaaaat
    67321 cattactcgt cctatgaaga ataccggcag atcttcacga ccaatgcacc tgcgtcgtac
    67381 atcaatcgca tgacccgatt tgatataaaa acgctgctcc ctgccctgtt gcaggtagaa
    67441 gaccgaacca gcatgatggt ttccattgaa tcgcgcgttc cgctgcttga ttatcggatc
    67501 gtcgagcttt cagcgtgtgt gcctccccgc tttaagtatg cgggtggtca atcaaaacac
    67561 attctgcgtg aagccagcaa accctttgtg ccgcatgaga tcctgcaacg gaaagataaa
    67621 atgggattcc ccgttccttt agatatctgg tatcgaaaag atccggtgcg tgcattcgtg
    67681 cgtgagacgc tcttaagttc tgcttcgatg aagcgaggtt ggtttgatgc acaagcggtg
    67741 agagagaccc ttgatcaaga actgccattc ggtcggacga tttgggggct gctgtgcatt
    67801 gaattgtggg cgcgagcttt tttagacgct tgaaattctg ataacaaaaa agtcactgtg
    67861 gatcaaattt ccaactgatc gatttgtttt tttaagacct ctgcggatgc caccaacgct
    67921 tgttcctcgg agggagaaag ctgtagttca aggacatgtt ctacaccttg ctcccccacc
    67981 acggcaggta agctgagata gacaccgcga atgccataat aaccgggcac aaggttcgaa
    68041 accgaaagga cagaattttg gttgcggaga atgctctcca caatgcgcgc caaaccggca
    68101 gctacggcgt aaaaggtcgc ccctttacgc tggatgatct catacgccgc attgcgcacc
    68161 tggcttgtga attcttcgcg ttgagagatg gggatctcgc agccaataaa gcggcagtag
    68221 gcatcgaggg gcattcctgc gatattcgcc gatgaccaga cagccacttc tgaatctccg
    68281 tgctctccaa tgatgaaagc atggatgttg cgcgcgtcga gcaagaagtg gcgcccgagt
    68341 agccagcgga aacgggctgt gtccaaaacg gtgccggagc cgatcacgtg ttcagcgggc
    68401 aagccagaga ttttgtgggt ggcgtaactg aggatgtcga ccggattggt ggcgacgaga
    68461 aggatcgcat cgcgcgtata ttggatgacc tgtgggacga tttggttgaa aatagcgatg
    68521 ttccgtttta gcagatcaag acgcgtttct cccgagcgct gagcgctgcc cgcggtgatc
    68581 accaccacat ccgccccccg gcaatccttg taatcaccct gataaatacg gatcgggttc
    68641 gccagcggga tggcatggtt caaatccatc acttcccctt ctgttcgttc tttatccaca
    68701 tcgatgagta cgatctcccc gaccaagccg tgcagaagca gggtataggc aaaagtggta
    68761 cccacctttc cactcccgac gatcacgatc ttatatgccc gatgttctgc cacgaaggtg
    68821 tcgttcaatg tttgttgttt cattttcttt tgtgacaaga gctcctcgct cgttctgttc
    68881 gttatcattc tttttgttcc ttacgcacta tgcgttgcaa tcatgttaga cactatccat
    68941 tcaatcatac aggcaaacag gcgcagacga ttattaggaa aatataaaca tttacattca
    69001 aagtaatcct caacatggta aaacatattc ttgatgagat tgagatatta aaaaggagaa
    69061 aggaaaagcg cgctcggaaa gaaccaatac atcttgaccc caagaacata actttttacc
    69121 agaaattgct ctcggttggt tctctattga ggcgatttca tggtagatag ttcaccctca
    69181 gattagatgg tcgttttttg ttgttttcag cgatacggtg aaccatctgg cgacctcaac
    69241 caccgaacag tgcaattttt caaacctgga aaattgtttt aatggttttg tttcgaacac
    69301 aggaaagcac gttcgtgaaa aagaaaagcc agcacgccct ccttattatt tcttttctcc
    69361 ttgttataat agaagaagta taaagcaaat tgcttcatcg ctgctgaact tcgagaaggg
    69421 cgaataaaat tacaatgaaa gcatgatcta tcatttcctt cctgttgatc tgttgttctt
    69481 taatgcagct atcatttcct tgtttgtcgc ggtcattatc cttatcaagc gagttgaacc
    69541 tggtggaact gcgttcgcgg ctgttatgct ttctctcgcc ttgtggttga tattccgggt
    69601 atgtgaaggc gtttcagagg aaattcccca aaaaatattc tgggcaaaat ttgaatattt
    69661 gggtatagcc accctgcctg tttgttactt tatttttgta agtcaattca gccggaaaga
    69721 ccgatggatc accaagggca acctgatcat tttatctctg ctgccagtgc tcaccctcgt
    69781 tttggttttt acgaatgaat atcatggctt aatatggagt gacatctatc ctgcttctac
    69841 cccacaggtg gaaagcctta tttacgagca tggatttttc ttctgggtgt attgggtcta
    69901 tagctatgtg ctgcttttct ttggaattta ccgccttatt gcaacccttt taaatttctc
    69961 aaaagaatat cgcttgcaga tgatagtgtt gattcttgct acactgttac cctggctggg
    70021 aaatcttctg tatattgctc gccttagccc aattgatggc atggatctta ccccggtggg
    70081 cttggcattt tcagggttgg tgatcgcatt atcgctttac aaggggcaga tctttgaagt
    70141 ggcgccggtt gctcgcaatg tcatcttcga tgccatgcag gaaggcattc ttgttctcga
    70201 tatcaaggga aatattgtgg atggcaatga tgcaacggca aagatactgg atataccgct
    70261 aaaaaactta ttgaaaaaac attttacgga atcgctttca cgatatccac tgctgataga
    70321 aaacctgcaa aaaataggga acaacaaatt tgaaatgtgt ttagatgaaa acaagcaaag
    70381 gtttgtccag gtgagcattt ctctgatcgt tacgaatatt aacccaacag gacagctcct
    70441 tgttttgcag aatatctctg aacgaaagaa actggaattg tatgaaagtg aacaacgaaa
    70501 attcgctgaa gctcttgcgg gcattgctgc cacattaaac agttcgttgg aatttgaagt
    70561 ggtgcttgaa aagatgctcg ctatcattca ccaggttgtc cctcatgatg ctgccaatat
    70621 tgcattattg gatgatgata agaaaatgcg ttttgtaaag ctcatcggat atgaaaaatt
    70681 tgaatcgaag aaggttattg aatctcttga ttactctata aacgatattc cggatttcaa
    70741 gatgatggct gaggaaaaag aagctgttgt tatcaccgat acccacaata gccctgcatg
    70801 gataagcaat ccaaaagtag cctggatccg ttcttatgtg gggagcccca ttgttgccgg
    70861 aggaaaaacg atcggcttca taaatgtaga cagcagtgtt ccaaacttgt ataccccgga
    70921 acatgcacaa cggctcaagg tatttgcaga tgaagcggct atcgcgattc aaaacgcacg
    70981 cattgttgag gaattgaaag agcgcaacca ggatctgggc atattgtacg aagtcggctt
    71041 agctatgacc agcggattgg acaatcaaga aataatcaac ggcttgatca aacagattaa
    71101 aaactttaaa aatattgata tattcttctt gatgttgttc ggtgaagact cgatgaagaa
    71161 tatgctgtac tggtatacat tgaatgataa aacaataaaa acgattgagt tgacaaagcc
    71221 gtggtcaggg aattttatcc atcagatcct cgaacagaag aaaacggtat atctaccgaa
    71281 ccaggaaaga caagatatca taacgggcat ccccgatgaa aaacaactct cgtttagcgc
    71341 aatgcgttcg tatcttgggg ttccgctcat gcagggaaaa gacgtgatcg gcatcctttc
    71401 ggtgatcagc aaacagagcg gcgcttttga tcagaaacag atccggcttc ttgaaacgat
    71461 cacctcgcag gtatccatca cgctccaaaa tgtcaaaatg tatgaacgga tgaaagagct
    71521 ggcaatcgtt gatgagttga caggagttta caatcggcgc tacttctata tcgctgcgaa
    71581 caaagagatt gaacgagcgt tacgctacaa gaaagccctc tctatgatct tgatcgacat
    71641 agatcattat aaggatgtca atgatcatta cggtcatatg gcgggagata aagtgctaca
    71701 aaagatcacg caggcgattc aaaaagaact gcgtggcagc gatatatttt caagatatgg
    71761 cggagaagaa ttcctcattc ttttgtctga tacaaaaggt gaagaagctg cagttgtggc
    71821 agagcgcatc agaactaccg ttgaagcttt acatgttcaa tataatgaag aagagatctc
    71881 tgtaactgtc agcttgggtg ttacccgatt aaccgctgag cgaaattcac tccaagaaat
    71941 catcgctgat gttgatcaag cgctgtatgg cgctaaacaa aaaggcagaa atcgggttga
    72001 atacatcgcg tgaacaacgt ttcgttaagc ctctgtaaaa taattgtaaa aattccgctt
    72061 caatagcaac cctttctaca ttaatgggat taatatatag agaggagaag cggcaaaata
    72121 cataccagag gtatatgatg gtaacgtatc aatacacatc tgagattgaa ctgcgaaaag
    72181 cgttgttaga tgagatcgtt cgggctgcag gactgccgct caataacttt tggcgcaaat
    72241 tcgtagaaat gattttctgg ctgccaatca accgcttttc aaagctgggt tcttattttg
    72301 accagatggt ttatcagcat ggattcagca aagccgttga tatcagtttg aaactattga
    72361 ttaatgatat tgtcgttctt ggtgaagtga aggcacctgc cacaggtccg ttgttggtag
    72421 tttccaatca ccctggcact tatgatgctc tttgcctggc agccaaattg cctcgcgatg
    72481 atgtgaagat tgtttcatcc aatatttctt tccttaaaaa tatgaagaat gttaatagtc
    72541 attttctctt tgcttccatt gacagttacg ttcggatgct tgtgattcgc catgctattc
    72601 gccatttgga aaaaggcggg gcgttgatca tttttccaag tgcgcacatg gaccctgaac
    72661 catcttgcat gcccggcgca atggaagcat tggatgaatg gtcgcgaagc atcgagattt
    72721 tcattcaaaa ggttccctcg actgttgttc aagtggcggt cgttagccgt gtgctgacct
    72781 ctcgctatct ttcccatccg ctcacgcgtt tgcggaaagg tcgcagagag cggcagcgca
    72841 tagcggaatt tatgcaaatc atccaccaaa tgatctatcg gcggaaattc aaccagaccc
    72901 ctttggtttc gtttgcggag ccgttcaaat catcagatat cggtcctgat gataagatcg
    72961 atcgcgtgtt gccctatgcc attaaccagg cgaaacaatt gatggtcgct cacctccctg
    73021 agaattatta aaacaaccaa gagagcacat acccaatctt tgatttccac gatattcttc
    73081 tgcatgtttt cctttagttt ttgaccgtaa agatggttac agcgtactat aatctacgtg
    73141 atgaccacga tgaaacgatc agccgtattt ctaagcttcc atgttctatc aaaatttcta
    73201 ttgctcatgc tggcatcctc gtgcagcatt cttccttttg gtgaggcatg gttccaggat
    73261 gataagacac ctggcacaca accgaccggt atcatttttt ttgatgattt tagttccaac
    73321 acatccggtt gggacagggt ggaaacagac ctgggcggaa cggactacgt gagtggaggt
    73381 tatcatattg tgatcaatga aaagaatacc gactacttct ctaccctcta ccgcacctat
    73441 accgatattg gtcttcaagc agatgctcaa tgggtggaag gtccgtatga caataatttc
    73501 ggtctgatct gtcgctttca ggacgaaaag aatttctacg ccggcatgat cagcagcgat
    73561 gggtattatg gaatcttcaa aatagagaac ggtgactata cggttttggg gcatgagacg
    73621 atgcttcaat cggaaaccat tggaattacg gattcacgaa aacagatcgc ttttcgttgt
    73681 tatcaagatt ttctattcct ctacgtcaac ggaattttac tggatgtaca gcaagacaag
    73741 acatttaatg ccggtgatgt agggatgatt gcaggtagtt ttgaagatgc gggtgtacac
    73801 atccttttcg atgatttcta tctcattgat gtaacccaga ccgcgaacca ggaaatgcaa
    73861 gaatgaaaaa gtatcctttc ctctttctgt tttttttaat gattctctct ggctgtcaaa
    73921 attttcttga tggggaagaa aatgcttcaa cactagtgcc cgcagtaacc gaaactgcta
    73981 ctcctacggt gtgggcaacc ctggctcctt tctcagcaac atccacaccg atagttttgg
    74041 caactctgaa ccccgttgaa ttacaagggg aaatcacttt tcagtctgat cggagcggga
    74101 acatggatat ctatgtttat gatctgcgct cagaatcttt atccaggatt acttcgggtg
    74161 agagcgcgga tgtctttccg agctggtcgt cagatgggga acagatcgta ttcgtatcgg
    74221 atagggacgg aaatccggac atatacattg tgaacagaaa tggcagcggc ttgaaacggc
    74281 ttacggatga tcctcatgat gatgtcttcc ccaccatctc cccagatgga aaaacgatcg
    74341 cgtttgtttc taatcgaggt gggaatgatg aaatctatac gatggatatt aatggagaaa
    74401 acctcacgca actgaccaat aatgtctttg ccgacggatt cccggcatgg tcaccggatg
    74461 gggagcagat catttaccag tctgaccgtg atgtcaatac ggagttgtat atcatgtcga
    74521 aagatggact tgacctgatg cgccttacgg atagcacagc agaagatgct caacctgcct
    74581 ggtcaccgga tggcaaacgg atcgcttttg tctccacgcg cacagaattc gaagagatct
    74641 atatttatca tctcgacacc cacgctgtcg aacaattaac gaactttcaa gcccagaccg
    74701 aagtgccaaa atggagcgca gatggcaaat atctaatttt tgcctctaat tatgagggga
    74761 acagggatat ctatatcatg gcagtggatt caagggcgat gttgaagatc accacttcaa
    74821 ccggggagga ttttttccct gactggaatc catgaaaacg cacgcctcca ggataatgct
    74881 tttcaagcgc attcagggat atattttcgt gcaacttctt atcgagagat tacgtaatag
    74941 aggtaacagg agtaaaaatg aaaaggctgt atagaaaacg aaatgacaaa atgctggcag
    75001 gtgtgtgcgc cggattggca gattatctcg atattgatcc gaccattgtg cgtatcatct
    75061 tccttatcct ggtttttgca ggattgggcg gggttctggt gtatgctatt ttgtgggtca
    75121 ttacacctct ggaaccgtca gccagtgaag acaccattga tgcgcgtagt aagggtgaat
    75181 gacaaaatta gaatagaaaa gttccggaat gaggcgaaag gaacttgaat gaaacggtgt
    75241 agaaagcaag ttcctttcgc cgttttttaa ccgtgttgat ttgcagtaga aacccgtttc
    75301 cccagtttct cacaatacat gcgatggtct gccagtttga acaatgcttc aacctctgat
    75361 tcacaatgcg ttgtggcagc tccgatagaa aaattcatac gatgcgcctg atcatctgca
    75421 ggtagggttt ggttatactt ctgaatattg ttccgaagac gggcaacgat cttcagcaca
    75481 gcgctctcct ccaggctgga tatgattacc gcaaattcat ctccacccaa ccgcgccaca
    75541 atatcatcgc cacgaaagct ctcttgcaag attgtagctg ctttgcagat gatggtatca
    75601 cctgcagcat gcccatactg gtcgttgatc tttttcagat tgtccagatc gcatacaatg
    75661 atgcttaaag gaaattgtct gccttgagaa atgcgtttga attcagcttc aaaatatgcg
    75721 cgattgtata gaccagtcat ggaatcgtgc atattcagga accgcattct ctcttctgcc
    75781 cattttcgtt cagtgatgtc cagaatcgaa agatggatgc gcgaccagct tttctcaaat
    75841 ccgttgatca aagacaggcg cacaatggtg tggatgatca aaccatccaa agaacggcag
    75901 ggcatttctg tttcgacctg acacgacccg ttgtagagag ccatgatgat cgatcgaatg
    75961 gattcagcag attgttcgat gaagataccg ttaaaatttt tctgtaggtc atccagatcg
    76021 gcagctttaa ataacaataa ggtcgcctgg tttccattga tgaccttgat cagggaggaa
    76081 aactcttttt caagatcagg cactttttcc aggaacagat tcagtttatc ctggctgttg
    76141 aattcaaggt tttcgatcca ctcttttaga gcggaaaaat ccatttccca catcgaaata
    76201 ggggaatcat ggaataaact ccgataacgc aattcgtttt ccttaatagc catttcattt
    76261 cttatgcggc tggtaatgtc gaggtacatt cccaaaactc caatacgctt ttccccgaga
    76321 aagattggaa ccccatagat tttaacatcc accagcctgc cgtcttttct gcgccttttt
    76381 cccgtaccgt agatggtgcc gccttgaagg accgttttgg tgaattcctg cgcctgcccc
    76441 aaatcaggat catccgcaag gatcgtatca aggtccttta gatagacttc atcttgggta
    76501 tattgaaaaa ttcggctgaa cgccgggttg atagaaatga tcttttgatc ggcatccatt
    76561 gtcacaatgg cagccgggtt gttgttgatc agtgattcgt agaagatctt ttgtttgagg
    76621 atttctgatt ttttttgact ttcaatgctt ttaaagagcg catgagcccg ggttcccgag
    76681 ccggttcttg tggaaaaata ggtcacgaag aaaattgcga aagggatgaa atcgatgatc
    76741 caaaagaaaa aattgctgtc atgaatttgt tgaatcgaat ttaatgagaa acgtgtgccc
    76801 gttcgaagaa attcatatgc atatacaaaa atgagaaaga gcaagcctgt caaaaaacct
    76861 aatcgagcat ttcgaccagc atgaatgcgc ttggcggcgt tagctttgtt tctcataata
    76921 gattgaaaaa attaacgagg ttcttatcct caatacagta ttctatatac ctcttcctta
    76981 aaaatgtcaa tggcttaaga aatatcacca gacccctaat agatccatac agaagggcaa
    77041 ggatgttttg catatataga taaggaagta gcgttccatc agtacgaaac cgttgaaata
    77101 tgcttgacta tgattctggt atactacttt taggttatgt tcggtgggct gcaccgtatt
    77161 gttgaatgcg gtattgttca attccccaat tgataaattg aaataatcag gtacaaacca
    77221 ggtgctatga caaacgaagc ccctttaaga gagcagcagc atgtcatcga agcttttgcc
    77281 gaactggctc ccaaatatga gaaaaagatg gattccgaat tgaatctttt ttggggctgg
    77341 agctatcgcg aactcattca aactttgctt gaagattatt ctttggttca tgcaaaacgt
    77401 atccttgacc tggcaaccgg caagttgatc atcccgcgcg caattgctgc tattaacccg
    77461 actgtgaaac aaattgttgg attggatatc actttccgca tgcttcaatt gggaaaaaca
    77521 atactgagca ggaattctcg cattcggttg ttgtgcgctt cagcgctgga aataccggtt
    77581 cgcccggcga gttttagcct gatcacctgc actttggcaa cccaccatat ggatgggaga
    77641 agattgctct ctgagatcca tcacatttta gattgggacg ggcaagtggt gatcgtagac
    77701 gtgggggctt caaaaacttg gatgaaccca atcgtgcgag gcgtaatgcg tgtactggca
    77761 tttttgtatt ttatggtgcg tgagaatgtt gcccgcgcgt gggcagaagc ggaggcgctg
    77821 tccaacatcc atacggcgga gcagtgggag gatgaattaa aaaaagcggg cgctgatttg
    77881 gctcatctca agccgccaca tatgcagccg aaagacaata tcattgaaag gttgataaaa
    77941 gtagattagt acaaactact atgatctgga attttgagtt tattgttatc ctccttgcct
    78001 ttatcagtta tttggcattg tacctggtcg tgtggttgac caagccaaaa accaaagaaa
    78061 aagaagcatt tcgattttac ttattgatga tggcgctgtg gaagctaagc gccatcatgg
    78121 ttgtaggaca gttgggagat atttatttct ggtttcgcat gatgaccgtt tcatcggtca
    78181 tgtccctgat cggcagccat cggttcgtgc agtccatttt gccgcaaaga acaagctggg
    78241 atcgttggat ctatgcctac atcgctttgg cggtatcggt cactctgttc acgaattggg
    78301 tggtttccta tgcatatatg aatgggaccg agctggtata ttcttttacg cctttggtgg
    78361 ggttggtggc gggtcctggt tatgcgctca tgctatacaa tacctaccgg ttgatcaaag
    78421 aatacagcca ggcaagaagc ccgcaaatac gcaaccgctt gaagtatttg atcttcggtt
    78481 ttatttttat catcattggg tcgattttta atttcaccga tctgggtcaa tatcctatcg
    78541 acatttttgc caacctgatc acggcgatcc tcatttctgt tgccatgtta cgtcaccaac
    78601 ttctggatat caatgtgatc atccggaaaa gcgttttata tgccattcca accagcctga
    78661 ttggcgcatt gtactttttg gtcattagtt ccgttgtcag agctttccaa ctttcagtaa
    78721 gccagcaagt gcttgtttcg atggtggtgg ctattgccgc agcgcttatc tttcaaccgt
    78781 ttcaaaaact gttgcaagat tggatcaacc gcatcttctt tcgtgagcgt tatgattcca
    78841 atctgatgct gcaacgggtg attcaaactg cgtctcaaga gatagatctg caacgcttgg
    78901 caggttcgat catcaacgag atcatctcaa ccatgcatat taagcgcgcc aggctgtttg
    78961 tttcgcatga agatggagga ccttttatcc tcatttcagg aagcgggttg aattattctc
    79021 gtcagaaaga gtttcgggtg gaccatcctc ttgtcttgaa aatggggaaa acacaagaat
    79081 ttttgatgtg gggcgagtta gaatcctccc ccttgctcaa gtcattgtgg aaagatgagc
    79141 ttgattacct ggttgatctg cgagcagagc tgttcatacc attacgaacc aaaaatcaat
    79201 tgattggaat tctggtattg ggcgaaaaaa tgtccgagac gccctattcg caggaggact
    79261 tgaacatttt aaccaccctt gcgagccaga catccctggc tcttcaaaat gcgaaattgt
    79321 attctctggc acaaaaagaa ctggttgaaa ggaagaaagc ggaaaaaggg ctgcaattgc
    79381 aattgaagcg gctttcggcg ttgcaagata tcaatgtcgc gatcacggaa aattttgatt
    79441 tgcaggtccc attggttatg ctgctcgatc aggtggtgag tgaactgaaa gtggatgctg
    79501 cggatgtttt gctctacaat cccgagaaca agagcttgaa tttcatcgcc ggtcgagggt
    79561 tcaacaccga tgcgctgcga tttaccagcc ttcagttggg gcagggcatg gcaggcatgg
    79621 ctgcccaaaa acacaagccc gtttacgtga aggatctgtc ggcagaacgc acaacgttga
    79681 aacagtctcc tttgcttgaa ggagaaaact ttgtctcgta ttatggattc ccgctcgttg
    79741 cccgtaatca gatcaaaggt gtattggaga tattccatcg ctctgtttta gaacctgacc
    79801 agcagtggtt ggattatttg aacacattga tttcggaaac tgccattgca ttggacaacg
    79861 cccgcatgtt caatgacctt gaaaagacca acatggagtt ggtgagcgcc tatgaagcaa
    79921 cgctggaggg atgggcgcgc acgcttgaaa tgcgcgaccg cgaaaccgaa gggcata
//
LOCUS       DAOSDS010000003        59987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_331_length_59987_cov_5.162384, whole genome shotgun sequence.
ACCESSION   DAOSDS010000003 DAOSDS010000000
VERSION     DAOSDS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 59987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59987
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_331_length_59987_cov_5.162384"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLL88_00770"
     CDS             complement(<1..661)
                     /locus_tag="PLL88_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00122.1"
                     /translation="MMIDRDSAQQAILESFRHELLEKSLLNQPAQEFVESALQKINEK
                     NGTTHHRSKVKQLLHKLLKYSDATKHEVHFQSVGTVQHIGNGVATLSGLPTVRLEELV
                     NFPNGVQGMVLNLDRRHADVILLGSDQGIRGGDLALATGKRLSIPVGFELLGRVVDPL
                     GKPLDEMGVINPSEFRYVERIAPGVIERAPVNEPLYSGTKIIDALIPLGRGQRELIMG
                     DH"
     gene            complement(651..1439)
                     /locus_tag="PLL88_00775"
     CDS             complement(651..1439)
                     /locus_tag="PLL88_00775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOJ00123.1"
                     /translation="MLDIDWVTILFEILNFVILTVILYFLAFKPIVRQFEERAREKER
                     LMDEMVRDREEAARYLAEIEARLNNLDAEIQKITDEAYEYNKNLQAELLSATQEEAER
                     ILQDALLDIRKKQSVDLKQNQSQLVDAILSISTHTLRQVTPLAVHDALIEELTKKIWD
                     LGKTNMMQVQSLRESLEGRAPVVSVITPLPLNSQQELNLVRTFSALADKDIDLDIATD
                     ENLIAGIKIRIADMIIDNSLAAHLDAMRGEVEQSLDQIDIKHDD"
     gene            complement(1443..1721)
                     /gene="atpE"
                     /locus_tag="PLL88_00780"
     CDS             complement(1443..1721)
                     /gene="atpE"
                     /locus_tag="PLL88_00780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOJ00124.1"
                     /translation="MENLEPGTLVTIVTILVTGIGVAIGMIVPSLMEGHAAVKALESI
                     ARQPDAANQIRTTMIIAMALIETSNIYVLLVMLVLLFANPLITRFFGG"
     gene            complement(1708..2415)
                     /gene="atpB"
                     /locus_tag="PLL88_00785"
     CDS             complement(1708..2415)
                     /gene="atpB"
                     /locus_tag="PLL88_00785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOJ00125.1"
                     /translation="MNVLTHPIAFYLLGLPVRSTVVMTWILMVVIVLLVLLIKKTNPA
                     ILETIIEFILNIVGDVLNVDNLYPYLSLLGSLFIFLISANLLSLLPGLTSPTADLNTT
                     IALSLIVFFSVHYFGMLKKGVWGYLKTVADPIFLLPIEVIGQISRSVALALRLFGNIM
                     SGDLIVAIIFSIVPLIVPLPLITLSMVTGVVQAYIFITLATLYIASAVEISQADEDGR
                     KTRKKLFRRKEVTNGKS"
     gene            complement(2416..2742)
                     /locus_tag="PLL88_00790"
     CDS             complement(2416..2742)
                     /locus_tag="PLL88_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00126.1"
                     /translation="MSNNVVTVGYGILWVVIGACSAFLSMLSIKSSAEKITSKTNTGT
                     SLVLLSGVAIRMLFLAALMYLAIRMNVIYALLLVAAFTVVRFILIVRLSKKNKQDANY
                     DDCEKP"
     gene            complement(2735..3007)
                     /locus_tag="PLL88_00795"
     CDS             complement(2735..3007)
                     /locus_tag="PLL88_00795"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOJ00127.1"
                     /translation="MDPRSGKRKHALTARDLKASSIGWELALPIVVGPVIGYFIDRRS
                     QTGVTYTLIFLGIGLIAGLSSLIRFIIDEFSFIRNGKNDLGNKKNE"
     gene            complement(3124..3384)
                     /locus_tag="PLL88_00800"
     CDS             complement(3124..3384)
                     /locus_tag="PLL88_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00128.1"
                     /translation="MSSDLLTLQVIDVNGPVANIDAITSIVLKLADGSMIGLRPGHAP
                     LIGALDDGQLRYVRQGTPAHLPVKAGILTVYQNKVKILTTAL"
     gene            complement(3374..4822)
                     /gene="atpD"
                     /locus_tag="PLL88_00805"
     CDS             complement(3374..4822)
                     /gene="atpD"
                     /locus_tag="PLL88_00805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOJ00129.1"
                     /translation="MIINKQSFETNDIGIIIRIVGVVVDVEFASGNLPSIHNALLVRR
                     KHEEDLYLEIQEHIDTNVVRTIAMGSTAGLRRGLPVENLNSPIKVPVGPATLGRVFNV
                     VGDVIDEKPPIKTKTLRPIHASPPAFSQQRVSRELLVTGIKAIDLLTPYAKGGKIGLF
                     GGAGVGKTILMLELMRNTIRRHSGIIVFAGVGERSREGNELWLELNEQGLIDSTVLVF
                     GQMNEPPGARLRLPYTALTMAEYFRDKENRPILVFIDNIFRFIQAGSEVSALLGRLPS
                     EMGYQSTLESEMGVLQERISTTSAGSVTSVQAVYIPADDITDPAVVSTFSHLDATTVL
                     SRRLVSLGIYPAIDPLQSSSQLLSPEFVGNEHYQIAMQVKATLARYDELQDLIAILGM
                     DELSKSDQETVIRARRLQRFLTQPFFSAEPYTGNPGVFVPLEENLQGFKEILEGKCDD
                     LPEQAFYMAGTIADVQQKTEQMMAGEEVEVEQ"
     gene            5321..5680
                     /locus_tag="PLL88_00810"
     CDS             5321..5680
                     /locus_tag="PLL88_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00130.1"
                     /translation="MRWQHPFILSCHSEGATRLKHLTISALIFRQITKKCITVRHSEG
                     AQRLRNLSISLLILDKSQKKRMHPFSVVYLKSWLRLGKIPRRANDGKKWRVVKLFLLA
                     QNQGKTCSQLPENKQEE"
     gene            6215..7858
                     /locus_tag="PLL88_00815"
     CDS             6215..7858
                     /locus_tag="PLL88_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ00131.1"
                     /translation="MPQYEYKVLKIPHVQQTYYCSFMACFGWQVQNIQESVDRVVNRS
                     MGFSNTTNYGSFNANTYFHPHTNSASTYGYSRNRGWGSQMNMQVTDVESSLTITFFRD
                     ANIPYLGELNDIEKRFFAATPAYLQRCKQKDRSKENQWPERIAVQTCADQGKAVLARG
                     KQQAQTQTTTQQRVEAQASTTSQEQPASIPSEPPSATIRETEVTHNVFQDNQPGLQIR
                     LNFNIKNRKGVQCRAVTYFFDEKHNPLQDVNQRFQTANGKVSVGSTFKPGFEDCYYNN
                     YILFLPYGELDQKDGDYRLGLNAKIYDEVTKAFLASSPDIFFHYIQNGQSMRGESIAT
                     PATKTAIPERPKTPARTSQKPAPSVKPAVTPERPSFEEYSATFCKSRGWDVLTEDRKV
                     FLQGLEIWYKDFRSAKPLAFHKKALDLNPKEPTYWQWVSSAYSDKLKFDEAIALLQKG
                     LKEISNDPVLLTSLGYAHIRKMDLDSAQKTADILSTLEERSAKYSYNWLLGMIAETRK
                     DFKTAIQYYDKADELSDPGNSTFLGMNQKRCRELMKQKN"
     gene            complement(8036..8629)
                     /locus_tag="PLL88_00820"
     CDS             complement(8036..8629)
                     /locus_tag="PLL88_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ00132.1"
                     /translation="MNVSESDPRSIRTRNLLRAALIRLAAEKAYDSLRIKDVTNGAGI
                     NRTTFYLHYSGLHELLEDCAHMLFEQMTAEIYAHKTDSQKNDAASLVPFVQSVFQHLQ
                     RYERFYRSMLGKFGDPFFRELFQELLSELIFEPMTMQKTDSPSDPRLEMNLRFFCAGF
                     TGIAVWWLENNIPISAEEASRYITSDILPDYLRLMSV"
     gene            8750..9715
                     /locus_tag="PLL88_00825"
     CDS             8750..9715
                     /locus_tag="PLL88_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00133.1"
                     /translation="MKTILDYIFLEPFPGDWLMQDIIAILLTFLVLVFIVRREKHAAI
                     VILEMAAFVFLYASIYENAAIVMGLYSYGRSLVMIGFVPASVPLIEACVLITGLWFLE
                     KTRVPQWAWAPIIGLFGMLQDFSLDPLAIRQIHTVGAVTSGRWNWLINPASDANILRI
                     PVFNFPGWMLIMFYSTICLLIGRWWYKKSGYRPLVGYIYPLITMVAALLLMISPLSNF
                     LLWLGPFFQKGQSIEWVMLAFHLVIPSALLIFLWRGKMTSRFTANDLPIFIVPTVLHL
                     SDILFTVLGGYTEILWIVLLASALQTAFLLFAWVNNRHAPITENQ"
     gene            10043..11776
                     /locus_tag="PLL88_00830"
     CDS             10043..11776
                     /locus_tag="PLL88_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RyR domain-containing protein"
                     /protein_id="HOJ00134.1"
                     /translation="MKTLQNYVQDFFALLNSTRETEAEQRFTNAIKMALLTFLDDESK
                     ENAATVYDIFFSTYRIVLEGSKSTFIDLLDVLSAYEERAATLIDKQRDHYIHSVNVFI
                     LGLCIYSQNSNFRTAFKKANIDGDKYPENYPNEQEEFLFRWGIAALFHDVGYPIEIIG
                     KQIESFLEFATNADHADSKGKIKAHLEFQNFRHLNSVAEVTPKKVFIKDFYEQNESSV
                     YIDLLQPIDLLAQKIHLSLAIPLDEIKNKLDSFVLDMAKFGFIDHGFYSAIIVLKWYG
                     YLIQKTNMNPMRFYNAVVDSASAILLHNYYRNACQKSFGCGPMNPVNHPIAYLLMFCD
                     EMQDWNRAGYGKIEKTRTQAASAKIFIDEKNFGITYLAGKGTFSRKFIDDKNELFNQL
                     LDIRAVFPGGLHIECDTLEVIFKEAREDVLVPRPMLENMELLAKAIHEDYIAARKKRN
                     EPIRVAEKFEDLDDSARYSNLRQAMNIGKKLQKVGYVLVSQDADGQEITQLDDDTIEQ
                     YAILEHDDWMEGKIKDGWSYASTRDDSQKKHNCLVPWSQLSEMYKEYDRDVARNVIRL
                     AAMVDMKVIKA"
     gene            11809..13200
                     /locus_tag="PLL88_00835"
     CDS             11809..13200
                     /locus_tag="PLL88_00835"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HOJ00135.1"
                     /translation="MKDPTIPSSSSESKSNLPFASYKGDGPYMFISYSHKDSGAVYPI
                     IAQFHGQRYNVWYDEGIEPGIEWPEEIANALNASKLFVVFISPNSVASENVRNEINFA
                     LAKKLPFIAIYLCETALTPGLQLQIGTKQDIRKYLLDEDTFRRKYLNSFESVFKKQET
                     KPWVPEPKPPEAERGKPDPVIKTPTTITTPTMPPAGALHFTCDVIRRAACEKLGLPED
                     RQLELKHTNVVTELRFLADAYGEPYLACSKGKERLMVYKKEGQPVFFFERGSIADLSD
                     LAYFKNLKDIRLIFHRFSDLMPLASLPIKILDLSCNQLADLAPLGAMPQLTNLSLDYC
                     TFESLTPLGNIKTLSFLSMDELSYQGFKELCALDLPNLQTLHIPFSKLESMEGISNLK
                     FLTWLSINDSIIFDFDEIAKLKNLTNLAISRTKCYNFSFLKELPLLNTLTVDEEQKAQ
                     IIELYGEQPAFIK"
     gene            13283..14308
                     /locus_tag="PLL88_00840"
     CDS             13283..14308
                     /locus_tag="PLL88_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00136.1"
                     /translation="MKQHNKHLKFILIPILFITTLILNACASEPQECVCVCQAGETAA
                     GTDATNSEEPVENAAAAIPAEGGEFVPCPIAFDSDRDGNLEIYTMDSNGENLVNLTND
                     PADDWNPDWSPDGSQIAFVSERNNERSIYIMDADGSNLRQLTNMPESDLADWSPDGKT
                     IAFTSKANGNFEIYIINADGSNEPINLTNNDAQDLVPVWSPDGSQIAWLSGDTEGWDL
                     FVMTSDGSNVRQLTDQGHVYNVKWSIDGNLYLDWENNVYGSFNCLMDPDGEILASGFG
                     KGGFVQYFPYWTLEGDKVELVSADMDAGNNEIYLIGDIFPDFILNITNNPGNDENPDW
                     PALCGLR"
     gene            14409..14795
                     /locus_tag="PLL88_00845"
     CDS             14409..14795
                     /locus_tag="PLL88_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00137.1"
                     /translation="MPKKLFTALLIGAGNRGTEIYSAWVLAHPDKLRIKAVAEPIEAR
                     RMAIASKHIVPTTTSIIPRTVVFDSDRDGNREIYIMDSNGENLINLTNDPADDWTPGW
                     SPDGSQIASTFIRGMGQGKAQNILCL"
     gene            complement(15081..16004)
                     /locus_tag="PLL88_00850"
     CDS             complement(15081..16004)
                     /locus_tag="PLL88_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00138.1"
                     /translation="MMSDILLAMISPIVFAGTIRIATPLIIGAVGGCFTNRAGTFIIA
                     YECFMLTSAFFATWGSYMTGSAIGGSLIGIIACIVMGIIFGMLVFHYKADALIISIAL
                     NFGAWAITTLLLQSIFGARGAFISEKIISYDQIHLPFLDGLPFITTVFNDKIILVYLS
                     YVIMFIGYIVMYKTTFGLRVRGVGINPTAAETAGVNLLKYKWVTLLIMSVLTGLAGSY
                     IPLSGVSMFTENMTSGRGFLCLAAVLVGQGNPIKAGLIALLFAYTDALFLTLTSFELP
                     TQLLSTMPYFAVIIVLLVNGIKEHKMLARVS"
     gene            complement(16004..17083)
                     /locus_tag="PLL88_00855"
     CDS             complement(16004..17083)
                     /locus_tag="PLL88_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00139.1"
                     /translation="MKRSLKINDEIKNSVIKYIAAFLAVIVIGSMLILLQGENPFNAF
                     REIYKGALGTKISFGSSLRYAMPCILVGIAASIAFKAGIFNMGVEGQLYFGALVAAVV
                     GYSYKFPPIIHIFLCLLAAGLAGLMYAFFPAILKLFFNVSELITTLMLNFMAKLLTEY
                     ITMWWIGHGVSNLGSNAIATPKIYDSAVLPNLIKGTSASYGIIIAVLIALVVAIFYKY
                     TIKGYELKQVGENLKFAKIGGVNAKSSFVSIFLLSGFIAGISGGVEVLGSYFRFTAQF
                     ASNMGWDGIMIAFIANKNPIAIVLVSFLWGALKAGSLNMERLTSLNKLTVNILLMLFV
                     LFVSVDYKVLFKEIQSIFKKGSVGG"
     gene            complement(17055..18605)
                     /locus_tag="PLL88_00860"
     CDS             complement(17055..18605)
                     /locus_tag="PLL88_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00140.1"
                     /translation="MKDDEYILELRKIRKTFGSLVANDNIDLKIKKGTIHSIVGENGA
                     GKSTLMNIVTCLHKPDSGEIIYKGQKINFKNPMEASKQGIGMVHQEFMLFNDLSVLEN
                     IIMGYEGGSNKIFIDKEECRKNIKEICNQYKFNVPLDVMINNLPVALLQQIEIVKLLY
                     RGADLLILDEPTSVLTPLGIEGLFSALKFLKNIGKTIIFITHKLKEVFAISDYITVLR
                     DGKVSGNILPSEINEAKLASLMVGREVILKAQKLDSVFGEKVLEVKDLSYIDNGSYRL
                     RDINFDLRTGEIVGIAGIAGSGQQKLIETVMGLLKPEKGSRIFLFGENVVEKSIRERR
                     LMGLGYIPQDRMNEGINTLSSIWENAIMGFHIVHGFKSKIFLDRKEVSSFTNQIIDKY
                     SVKVQSIDDKVQSLSGGNIQKLIVGREFLQGYKLLVIEDPTRGLDVGAIEFVWKKIIE
                     IAATGVAILLISHDLNEVMQLSDRIFVMYNGLLKEGGLHGELSENEIGLLMTGGESVY
                     ETFLKNKR"
     gene            complement(18803..19858)
                     /locus_tag="PLL88_00865"
     CDS             complement(18803..19858)
                     /locus_tag="PLL88_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ00141.1"
                     /translation="MKKRSSLIVLLILLTVFASCTSAETEVVEEEPVGEAVEEEEVEE
                     VTKKAAFITESPLGNPFTDLIWSGFEALESEGWEVKVIEALDSAEYAESIRALAGDGY
                     SVIMTFADAVSNVAVDLADEIYELYPETRLFLLDTYMEHGKPNCTSVSVDPFESSFVA
                     GFVAAKTTETGTIGWIGHTDILKIRRFRDGYIAGAAYANPDVEVVYSFTGDYLDPNKG
                     LEAGAAMIANYPVDIIYQSCYLAGPGVIEACANGGIRCIGVDDWQGNLDPSVFWSAIK
                     PMDNAVITLASKWAEGYEFPLYLDFNIGSGGRVYDERDFEKLSPELQAEVLQLVEDIR
                     TGEVDVYEGFEEYRLDY"
     gene            20113..21402
                     /locus_tag="PLL88_00870"
     CDS             20113..21402
                     /locus_tag="PLL88_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOJ00142.1"
                     /translation="MNTKKDIKNDQLLDISLLIGGKWKLIIIRELIGKTMRFGELKRS
                     IGSITHKVLTDNLQALEKDGIIKRVVFPESTLKVEYSLTPAGYKFAPLHRYCISCYYD
                     YLFALNQDKLDNVENDYRSELKTNFYEPDSHLKGKIERKNTYTERISRTKELIKNADH
                     IIIGTGSGIDSNNNKIDHFSKRKAQELFPNFFDLGLQNIDEMINAFSNIYPENENEYW
                     EFWAKYIWELRYNRPVSQSHKYLADIVKDKDYYVISTNSDGQLEESRLSKQRIHLPLG
                     NYSYLQCTFPCHEMLYESKEYIEKILNHLASGDEMNNAITPHCPQCGNYLIPNHYRVK
                     TISLVESNNMATHSMFCDFINKIRNDNNVFLEIGCGLRLPSIIRHRFENYLHEFHNTY
                     LIRINRRYPDLEFPKHKDKFISFEEDTKLVLEDLSIE"
     gene            22090..23142
                     /locus_tag="PLL88_00875"
     CDS             22090..23142
                     /locus_tag="PLL88_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ00143.1"
                     /translation="MTERENKILGSLLGAAVGDSMGAATETRSTDMIIEKFGGLVVDF
                     VEPPDDNLARGTEAGVVTDDFSVAYYTAEEILATKSPVSKEIAEKALLHWSTHPEYTR
                     YIGPTTQVSISKIKGDLEQGFEDSRFQNLLCNNKRATNGAGMKAGIMGLFNPCDLDRA
                     IDDAITMCLPTHDNTIALSGGCAIAAATSKAMSSKVSYMEVIEAGIYGASEGYKKSKE
                     VARPVAGADIEKKIRLAVELGLKYQDNFEKSMRIISEIIGGGLYAYEAIPAVFGHIAA
                     CKGETMESILMGVNGGDDTDTVACMTGYIVGALNGAEYIPQKYLSLINGKNNFCLEKM
                     ALNIVEFTKEQEKLSA"
     gene            23139..24179
                     /locus_tag="PLL88_00880"
     CDS             23139..24179
                     /locus_tag="PLL88_00880"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ00144.1"
                     /translation="MSNLYDKVFGCLLAAAIGDAMGSPLETRPVYLIKKHYGNGNFVY
                     DYMKPLDDTLGHGLPKGLVTDDFSVAYISGKHFIKNGGHITKQVASDALVDWKESEDT
                     KVYYERWCGPTTGKSIAKLSGVKIDDPRDYMFCENRTATNGAGMKSWIAGLFNPGNIE
                     KAIDDAITMCHVTHNNPIALSGGCAIAATVSKCLIKDVTLDEVVDAGLYGAHEGYNRT
                     LRITRPSAGASVEQRIKLAVEIGLKYSNDFEKCILEMTDLIGTGLNANEAVPSAFGYL
                     VAGGGDIMKTIYLAINSGNDCDTTAIMAGAMVGAFSGGSDIGPYHLKLLTETNPFMDI
                     PDLAKKIVDLTI"
     gene            24229..25146
                     /locus_tag="PLL88_00885"
     CDS             24229..25146
                     /locus_tag="PLL88_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ00145.1"
                     /translation="MKKSNKYLVFISVLHMDEYYYTEKWPQVGDKTILVPGERVPGGS
                     ASNAACVSSKLGAPVFFYDVLSKSNTNKFLLEDMKKYKVNVDYVNFVDNLDDSKCLII
                     ATPGEKTILGIIHPKPSTPLSDSHIRLFNSAKYIYTTLGFYYFIPDAHSTFNNFKKQG
                     AKLALDVEFGVRDQNDISILESANILFFNEFGFSANRKNLSDEDYLNYLLGKGVELIV
                     ITLGKRGCKVKTKDLEFELPAYEIEVIDTNGAGDTFNGAFLYGLLKDWQLKKTTNFAL
                     AAAAYCVTKTGPRGGAVPEKTIREFLKQL"
     gene            25174..26124
                     /locus_tag="PLL88_00890"
     CDS             25174..26124
                     /locus_tag="PLL88_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ00146.1"
                     /translation="MFDVIGLGTIAMDIFFHVDNLPIEDGFALIQKSNLLPGGSGTNV
                     VTQISRLSGKCGYIAQIGDDDIGISIIESLVKENIDTDGIRIKNGATSLHCKIIVDPN
                     GRKFILLEKGNAFDKWAFSEINQNYLFEAKIFYTDLLPPQPAVRSLKIAKKAGLTTVF
                     NLQMGIQQMELMGIKKETIIDVLEYVDIFAPCSNGFLQLCNSSELDNCHKYISHYFDG
                     LLLCTLGENGSKAFYKDNEPIFIPAMHIKPVDTTGAGDSYLGAFIYAFLIANRKLQQS
                     MKFATACAAYTCERIGARSSPNLEQALIMEKKFDKELEIE"
     gene            26117..26953
                     /locus_tag="PLL88_00895"
     CDS             26117..26953
                     /locus_tag="PLL88_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOJ00147.1"
                     /translation="MNNTNKMNQFSKIFKDKKPIIGMVHIPPLPGAPLFNPPMTWDEI
                     IKFTLLDAQALIEGGVDGIQIENQFDIPYNLSKNIGAETTAFMTAITCNIRFIYPEFP
                     LGIHILLNGCIQALSVAKAAHADWIRAYNVANAYISNSGYIGASGPALMRFRNTINAN
                     NVMIFGDFQVKHGSHAITADRSIEDKAHDIETSLCDAAIVTGNATGSPPDISTLKKIR
                     EKISIPMLIGSGLSQKNIRKILPFADGAIVGTSLKKDGVISEHIDIQSVRELTKLAKS
                     IE"
     gene            27090..28505
                     /locus_tag="PLL88_00900"
     CDS             27090..28505
                     /locus_tag="PLL88_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ00148.1"
                     /translation="MPKKLFTALLIGAGNRGTEIYSAWALAHPDKLRIKAVAEPIEAR
                     RMAIASKHSIPAEHQFTSWEEVLSKGKIADAAIICTQDQQHTAPALKALEAGYDVLLE
                     KPMAHRLEDCVALVQAAEQHGKLLQIAHVLRYTDFFQRVYQTVNSGELGQIITVSHRE
                     NVSAWHMAHSYVRGNWRRADQASPMILAKSCHDLDILYWIFGSRAKTLSSVGNLKHFR
                     TENAPAGAPMRCLDGCPAAESCPFYAPTLYMDLEQIYHGLANAQNKGIRLAGRLMQIS
                     PEVARAAARVVKPLRQITEYEGWPRSVITDNPNDPQSIYEALQSGPYGRCVYHCDNDV
                     VDHQIVLMELENGISVSFTMHGHSFEEGRTIRIDGSKATVLGKFGWDHSFIEVREHRG
                     AKVNRIELPNNVEQGGHGGGDAQLVENFVKALGGVQGAALTDARSSLESHLMAFAAEQ
                     ARTKGSVVDMAAFRANAEKLA"
     gene            complement(29394..30359)
                     /locus_tag="PLL88_00905"
     CDS             complement(29394..30359)
                     /locus_tag="PLL88_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00149.1"
                     /translation="MKSRSVNIFWGIVLIALGSVFLLNEWGVIQLYGWSNLIWALVFG
                     AAAAFFLTTYLLKNGQEWGWLFPATILAGIALLIGLEGTRLGRVLSATPVLLGVAAPF
                     MVAYFRDPESKRWALIPSWVFFVVSAIVLFERYLNGNLVGALVLYSIALPFLYVYVTD
                     RSRQWALIPFIALAVVGTIPLLGIFVSGAWFDVAVVLLMAVPFFVVYFWAKKNWWALI
                     PAGVFVSIAVGLLIERIFHVPFLAGIFLGGLGLTFGALWLLREKYFTDWAKYPALVLL
                     GIAAIVLLTEEATSLVGPIVLVVVGLAVLIISLLKRSGKNGTPER"
     gene            complement(30548..30769)
                     /locus_tag="PLL88_00910"
     CDS             complement(30548..30769)
                     /locus_tag="PLL88_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00150.1"
                     /translation="MDNEKDLNEKECCKHHTVRHDGASGAVYGLGFIGACIYFIGNAA
                     TFWLGVLGFLKALVWPAFLVFEALKYFGL"
     gene            complement(31036..32340)
                     /locus_tag="PLL88_00915"
     CDS             complement(31036..32340)
                     /locus_tag="PLL88_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00151.1"
                     /translation="MRLEKRLTKEKKYGSHCYQLRIFLILVFLPGIFSACTSAGSAAN
                     TPTSVPTPTVEPPNPETALEKALQRFDEVQSFTVTGSGSTQPSDSDRYTSFSTEYIDQ
                     TKQTRLKEYGTTLDFDIKHTKLCMSSACYSADATGLLKPVSSTYIAYYPEIESLDSNL
                     SKISDPNYTYVGENTIDEVRVFEYEIPITEEMIAMYKEEPDPNITYRILIEPYPVLTL
                     YVNAEDGYLVRMVETYRATYDFADDDYYSDCVLEKDFSGWNTTTFTIPEYVDAQNSDW
                     QDYSGVYSSLVTFQFPKVYRLDESYSYPILRTPSGSKMIFKMYANIASLSSLDGAERD
                     QETGVAVCYGVTQLWLVPAYSGTPVIENTEWFNMDFCKTVISSDDGQVANYLFNEPIE
                     FAWAHGRILPVTYLVSITPAEGEDAGTLFWDVIQTIHFGASE"
     gene            complement(32646..33566)
                     /locus_tag="PLL88_00920"
     CDS             complement(32646..33566)
                     /locus_tag="PLL88_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOJ00152.1"
                     /translation="MARVIAVNISEKKGTFKLPIKQACLKIDHGIVGDAHAGNWHRQV
                     SLLARESIDKMTALGVKDLTPGKFAENITTEGVELFTMPVGTRLKIGEVLLEVTQIGK
                     ECHQHCQIFEQVGKCIMPSEGIFAKVIKEGSVNAGDEITIFPSIHVAILTISDKGSKG
                     EREDQSGPAIAATIKNHASVTQMAIIPDELEVIKTTLVQWADSGEVDVVFTTGGTGFS
                     PRDVTPEATTAVVERLTPGIPEAMRQASMEKTDRAMLSRAIAGIRGKTLIVNLPGSPK
                     AAVECLEVFLPVMQHAVEILHGDAHDCARK"
     gene            complement(33570..34517)
                     /gene="moaA"
                     /locus_tag="PLL88_00925"
     CDS             complement(33570..34517)
                     /gene="moaA"
                     /locus_tag="PLL88_00925"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOJ00153.1"
                     /translation="MIDQFGREIRYLRLSVTERCTLRCVYCRREEGICPKAAELSSVD
                     FLRIAKACVDLGINRIRLTGGEPLLRKDIVEMVESLSSIAGVDELTMTSNGQMLAEQA
                     ESLKQAGLTRINVSIDSLDPQKYRKMTGGDLNAVLAGIDEAVRVGLLPVKLNMVLVKG
                     VNDGEVDDLIALSKDQPLDVRIIELMPMAELGQDRSLVINNDELIAARPYLKPVAPRY
                     AGQPSRDFTIDGHQGRVGFISPIYHRFCSTCNRVRVMSDGMLRPCLGNNSEISLKEAL
                     LAGDAALKETIRDAIFNKPARHCFELGFEPQKDMVRIGG"
     gene            complement(34514..35002)
                     /gene="moaC"
                     /locus_tag="PLL88_00930"
     CDS             complement(34514..35002)
                     /gene="moaC"
                     /locus_tag="PLL88_00930"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOJ00154.1"
                     /translation="MELTHINKNGEVAMVDVSQKDSTDRMAVAHAAVRMKETTWNLVL
                     AGEMKKGDVFATARVAGIMAAKRTAELIPLCHPISLTHISVDIQPKTPGRVEILATAK
                     CTGTTGVEMEALCAASVAALTIYDMCKAVDREMEIISVRLVEKDGGKSGHFIHANARG
                     EE"
     gene            complement(34993..36912)
                     /locus_tag="PLL88_00935"
     CDS             complement(34993..36912)
                     /locus_tag="PLL88_00935"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HOJ00155.1"
                     /translation="MPFNYLTNTPLDEAVEQYLKILLEHGLHYKTEVILTQDALGRVS
                     ANAVYAKISAPHFNACGMDGIALDSRKTFGATETTPVRLRESDFVWVDTGDPLPAGCD
                     AVVMVEDVVEEGEFYVLYAAAVPWQNIRQIGEDISAGDMIIPSYTLITPSCMGAMLAA
                     GVLQVEVVKRPIIGIIPTGDEIVAPTENPGEGDIVEFNSTIFSGMLTEWGCIPRVFPI
                     VRDDADLIQNALADAVNECDGVMINAGSSAGREDYTKDAIQKIGTVILHGIAIKPGKP
                     AVLAMVVRENPSYCIPVIGVPGYPVSGIMIMEMVFKTIIEKLTCRTFERAETIEALVS
                     RRLTSSLKYREFIRARLGWVNGKMIAVPLNRGAGVVSSFVKADGIIDVPQNLEGYEAG
                     EKVNVHLLRDLEEIKRTLVISGSHDPLLDEVTDIMQRDFPGCTIASSHVGSMGGIMAV
                     KRGEAHLGGTHLLDEETGIYNIPYVKRYFPDGGVVLMECVGRVQGLMVARGNPKGIKG
                     FSDLVDLSYVNRQKGSGTRVLCDFLAKQNGVDTSLIHGYEREEFTHTAVAAAIAAGTA
                     DAGLGILAAARIYGLDFIPIAEEEYDLLIAADAYETEAVGRLIIIIKGKEFARRLNAL
                     GGYILKNPGRIKRWN"
     gene            complement(36916..38124)
                     /locus_tag="PLL88_00940"
     CDS             complement(36916..38124)
                     /locus_tag="PLL88_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOJ00156.1"
                     /translation="MLAVKALADVQMLVKDYFGEIRTAPEMISFENAADRILAEDIIA
                     RECVPAFPRATVDGYAVKCSDIFGCSDAIPAMLKLVGEAIMGKHTDRNLDSGQCVSVP
                     TGGEVPEGADAVVMLEDVEDFGDGTIGIRKPVAPGSNLIFPGDDGKAGELVYRKGKKL
                     SAKDIGTLAAMGIIAVPVMKLPRIVIISSGDELVDARAVLPLGKIRDVNGPMLKMAVG
                     ECGGEAAFSGIVIDDQHALRDAVLQTINECEILVLTGGTSAGTKDAIPEVISALGELL
                     VHGVAIKPGKPTIVGKIDHVPVFGLPGNPVAAYLMFLLLVKPLVLSMLGAVGIETART
                     MPLARTVSSNQGREDLIPVSIKNGLADPIIGKSGLITTLANADGYFRIARDQEGLKAG
                     EPVKVFLFEE"
     gene            complement(38153..39364)
                     /locus_tag="PLL88_00945"
     CDS             complement(38153..39364)
                     /locus_tag="PLL88_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOJ00157.1"
                     /translation="MLKTKPVSEVKQIIQHTFGKMRTDSETVPLQDALWRVLAEEMIA
                     SAYIPHFDRSTVDGYAVKVGDLSDCSESSPARLALVGQSKMGEHTEFHIENGQCAYVP
                     TGGEVPTGTETMVMLEDTRELGEDLIGFTKPYREKMNMIFRGEDMEPGDRVIAAGKRL
                     KVADIGTLAVMGIVNVPVMKRPRVGIVSTGDELVPATDLLTRAGMIRDVNTPMLQHAV
                     QVSGGKPHCYGILKDDQQGISDLIKKAMTECDLLLVSGGTSMDARDTLTAIVADLGEV
                     IVHGITVKPGKPTIFGAIQGKPIFGVPGNPVSAYFTFHTFVRPLLHSFLGTPVVDRKV
                     ILPLSRKFITNHGREEYVPVIVKDGMAYPVFSKSGLITTVSCADGYMVVPRDQNEVEE
                     GTWVEVTFLDG"
     gene            39580..40413
                     /locus_tag="PLL88_00950"
     CDS             39580..40413
                     /locus_tag="PLL88_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00158.1"
                     /translation="MEIKKGAANHNSKANHLWSLLAAPLLLFILLPIVSLFLRSSITD
                     IIHNLNNRQVSQAITLSLSTSIVSTLLTLLFGTPISYLLATRRFRGHNFIDTIIDLPT
                     VLPPSVAGVALLLAFGRNGVIGGLLAKAGITIPFTFIAVVMAQMFIASPLYVKAATIG
                     FAHVDKQLIQAAELDGANRWQIFRYIFIPLSGKSLLSGSVMTWARALGEFGATIIFAG
                     NFPGRTQTMPLAVYIGFEISMNVALTLSIILICFSFLTLIIVKGFLHTRLESDETIVS
                     Y"
     gene            40682..41539
                     /gene="modA"
                     /locus_tag="PLL88_00955"
     CDS             40682..41539
                     /gene="modA"
                     /locus_tag="PLL88_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ00159.1"
                     /translation="MRSKFLPICLIFTLLATVACSTQAAAEPATTDVPSEAATAAPVE
                     ERTLTVMAAASLTESFTELGLIFESQHEGVTVAFNFAGSQALAEQLAQGADVDVFASA
                     SNKYMTAAVDAGRVTADASSVFAYNRLVVIYPKDNPGGITELKDLAKEDLKVDLADAS
                     VPVGQYALDFLDKASADANFGSQFKEDVLNNVVSYEENVKAVVTKISLGEADAGIVYV
                     TDITADAAELVGTLEIPDELNTIATYPIAPIADSKNADLAQAFVAFVLSSEGQAILAK
                     YNFVSVLSN"
     gene            41813..42391
                     /locus_tag="PLL88_00960"
     CDS             41813..42391
                     /locus_tag="PLL88_00960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOJ00160.1"
                     /translation="MKIHIIIDHPDPASFNHQIMNTFIEGIDKNKHELDVLDLDADQF
                     NPVMSKEEFKAYNRGEYLDPKVKEYQQRLKSADYLALFFPIWWMVMPARMKGWIDKVL
                     LPGFAFSKGANPEPLLSHITSAVIFTTTAISDEKHRKDFNNALDWVLCKGTLNFIGIR
                     NTQWLNFGEAGIASPEKYQEWLAHVLDFAKNL"
     gene            complement(42455..43549)
                     /locus_tag="PLL88_00965"
     CDS             complement(42455..43549)
                     /locus_tag="PLL88_00965"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ00161.1"
                     /translation="MMNPYERHLAVINNEEPDKVDVVFAAGLRGGPTRGMLRRLTARG
                     MGITHIIPPYKPMFFFDHMINPFLKDIAYTQKSFCQDNVWMIQHIFETPVGTVDSIVG
                     INPGVNVLTHSAQTFFVKEVEDWRVINFVFEKMLENMVPNYDELEGDQQDLGTDGTTI
                     AVVDKSPFQRAWVELASVERAAIDFKTKPDLLLEYLDIQHRFHLKAAEITAGCPAQHI
                     TLIDNITNLISPKYYREYCMPIYDIYVNALAGTGKKFAVHHDGLLRHLAKEIHETKID
                     IVDSFTVPPIGNISLTEAKEFFPEKILSVNLPPHLAYANMDELHAGYEKILQEHGKKQ
                     LIIEHVEDLPEESLENHLSAALDVCGYPTQ"
     gene            complement(43546..44496)
                     /locus_tag="PLL88_00970"
     CDS             complement(43546..44496)
                     /locus_tag="PLL88_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00162.1"
                     /translation="MDLDFILDVLGTGLRMATPFILAGLGGLVSLSAGDLNISLEGFM
                     LVGAFFAVLGSYFFESYVVGICFAVLATLLYGLLFGLFTIKLKADVFVVGIALNLLAS
                     EVTVFFSRLFFNVRGSFSDPRIEGIPNIHLAFLDKVPVLNDLLNNHSAFVYVSWVMIV
                     VFWFLIYRTPYGFYFRAAGEHPEALETTGIRVNKIRWIANMICSVMCGLAGAHLSLGY
                     LRLFVEDMTAGRGFIALSAVIFGGSNPVTMAITSLLFGLAEGISLRIQNMGIPSQFTL
                     MLPYLTTILALVIRAVRKSRKTALIASIAAAEQKIEGGTE"
     gene            complement(44496..45560)
                     /locus_tag="PLL88_00975"
     CDS             complement(44496..45560)
                     /locus_tag="PLL88_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00163.1"
                     /translation="MKNSKRTVNSLTSIVGVFIATMLFGALIMVVIGKDPLQAYAAMF
                     SYALGDVSAIANLMNRSMALILSALCYAVASNAGIYNLGGKGQIELGAIAAAVIGYKL
                     IGLPIYIHLPLCILAAVLMGMLGAAVPGYLRVKWKVDEVISTIMLNSIFFMFTSYLAN
                     YPFRDVTRWSGTTPPVEESAKLPYLVQSIDLRSGIIFGLVAAVVIYWIFNYTNQGYRW
                     KMIGLNPRFAQYGGVNVARDQMKAMALSGLLSGLTGAVLVAGSNYRYWESIAGPYGWD
                     GVLVAMLAKNNPFAIVAVSFVFSIFKNGALGMEQVANVPSDLTGVLLAALILFITGRE
                     FVSIIFRKRKHLVLVDGEAK"
     gene            complement(45557..47110)
                     /locus_tag="PLL88_00980"
     CDS             complement(45557..47110)
                     /locus_tag="PLL88_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00164.1"
                     /translation="MTTETLIRLENISKTFDGGVQANRNANLVVKKGEIHAIVGENGA
                     GKSTLMNILYGVLQPDNGSIYINDKEVSITSAREAKKLGIGMVHQHFMLVPSFTVLQN
                     VILGYEPRQNLVFVDNREAEKKVKEICDYYNLSIPLQSLTQDVSVGIQQRVEIIKAIY
                     RGAEILILDEPTAVLTPFEAEELFRICRDMVAKDKTILFISHKLKEVLALSDKVTVMR
                     KGETLVTLDTQDTTEPELARLIVGREQLWTVRKQKSSLAPQTVLETRNVSVRKTAHSF
                     CVKDVSLQVHAGEIVGVAGVEGNGQSELMEALAGLTPVDQGEVFFNGSSITNKPISNL
                     RALGIRLIPEDRMTTGASVKASIKENLVIDRNDCAPFISKPDVMNWKEIENHSKKLIE
                     KFSIVAQGPDTLVGTLSGGNLQKVVVARELSGKHQCLLVLQPARGLDIGASEFIYEQI
                     LDARKAGNAILLVSADLDELFLLSDRIVVIYEGEVVANFLPDEVTPEEVGLYMTGAKR
                     TTPNAGSTL"
     gene            complement(47191..48246)
                     /locus_tag="PLL88_00985"
     CDS             complement(47191..48246)
                     /locus_tag="PLL88_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ00165.1"
                     /translation="MKKHVLPLIGLLLIAAMIFTACSTAEPAEAAEPVAESETTTDTE
                     PITVGVFVADSFGDRAFYDIALGGIESIEEDFGAVTHTYEGKLDYENYKTLLSNAAKT
                     DDIVFVLGFEAIDAMLEVAPQNPDTLYIYLDGVLDSADVVSLGYKDHEGSFLAGALAG
                     LLTTRTDVNFINEDQVIGYVGGVDSPVMQRALWGYEQGLKYVAPEGTLEYIFVGSWVD
                     PAKGKEATLALMEMGSDINFMYAGLSGEGGFNAANEGAETWIIGGGFDQRWLAPDYTI
                     ASVMKACDVSIHDLTEMYLNGELQKGDTFNWGVKEGGMWLTMSEDIVPADIVAEVNDL
                     QAKIANGEITVTEFRDQ"
     gene            complement(48318..49493)
                     /locus_tag="PLL88_00990"
     CDS             complement(48318..49493)
                     /locus_tag="PLL88_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ00166.1"
                     /translation="MENHSEIIFQRLQVGLNENCSIASQIADQIRWLIVVNEIKAGDI
                     LPSVRTLADHLHVNLHTVRAAYRLLEETKLISTRQGLGSIVLDYDPSKTVSTHMLPTN
                     TFGIIVPDLQNPFYPSFLSGAAKVASAHNVLMITCDTREKNTIGKAFFDMLIAKRVDG
                     IIISPLGTDPEPSDFFDYGTFYDFPIPLVFADRPNVKGYSVLLDGKGAGYKGTQHLID
                     HGHRKIAMLTGNLSIPTLYTVYEGYREALEKNSLVLDDRLVIDVKEFSYQAGYRATVQ
                     LIENHLLPSAIFAAGDMLAIGAMKALREHAIRMPQDVAIVGYNDIDVCDYAAPSLTSI
                     TIPMEKMGEESAKLLLALMKKQKVPRQPIMMPTELVIRESCGCHRPDRKVLGVKKEV"
     gene            49613..50248
                     /locus_tag="PLL88_00995"
     CDS             49613..50248
                     /locus_tag="PLL88_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOJ00167.1"
                     /translation="MSTSLDAISQGIIEGDMTTVTALVEQALQELIAPHAILDAMTKA
                     MGEVGRLFEEGEYFVPEMLVSARTMQNGLDVLQPALTSADTKAKGKVVLGTVKGDLHD
                     IGKNLVAILLKGAGYEVEDLGADVAPEVFVDKVKENGKCVVALSALLTTTMINMKTVI
                     EAMSAAGLRQEVKVIVGGAPVTQEFATQIGADGYAADANKAVRLVDQLVSG"
     gene            50304..50783
                     /locus_tag="PLL88_01000"
     CDS             50304..50783
                     /locus_tag="PLL88_01000"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOJ00168.1"
                     /translation="MNIDLSMLEPIVKLTNPDQVGMVVDDAHKAASSFARIFGADPVE
                     VLDWPAPGFDPESYYYGQPTRWSMRVGFFQIGNLQWELIQPLEGKSIFADFLSEHGPG
                     LHHVRFTEPHFEQQVAALQAAGIRMISNGIGIHQVSRWAYFDTRSLLQGYLLELRKN"
     gene            complement(50815..50949)
                     /locus_tag="PLL88_01005"
     CDS             complement(50815..50949)
                     /locus_tag="PLL88_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00169.1"
                     /translation="MIADEPGRSAGMKAHQSNPGKVFAEMPPEKERSFASAFLRHTGE
                     "
     gene            51170..51658
                     /locus_tag="PLL88_01010"
     CDS             51170..51658
                     /locus_tag="PLL88_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00170.1"
                     /translation="MSIKDQVTPEQWKALYNAISAASTYVSTASGGGLEMFQEAFSAS
                     KFMMESSQKAGGSGYGSLVDEILAVMKGMNLNDAKADAHQYQSKDIAALRAEAKQLVV
                     DGAAAAAALPDGDGYKRWLVEMARKVAETKTGGILGFGGQAVIDEKEEAAIAELTELL
                     GI"
     gene            complement(51892..52344)
                     /locus_tag="PLL88_01015"
     CDS             complement(51892..52344)
                     /locus_tag="PLL88_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00171.1"
                     /translation="MNKKIILISVLIAGLLLSACATETAEPAAEEAVAVAEPALILSG
                     AVEASWTADDLNAMTQVEAEYTNKDGETTQYSGVLIRDLLTAAGVADYTKLTLVASDG
                     FTAEVTSEELAACANCIVAFDDDGTLRSVMPDLSGKQNVRGLVEITVE"
     regulatory      complement(52453..52572)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            52643..52846
                     /locus_tag="PLL88_01020"
     CDS             52643..52846
                     /locus_tag="PLL88_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00172.1"
                     /translation="MLAAHRYLQTQIGKVEVADPQVAVLSTGTPAGAFHAQKCRFADF
                     ASQDKHGEMQWAGRAMPAFLACQ"
     gene            52950..56657
                     /locus_tag="PLL88_01025"
     CDS             52950..56657
                     /locus_tag="PLL88_01025"
                     /inference="COORDINATES: protein motif:HMM:NF019912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00173.1"
                     /translation="MKKRHLSRFCFLATFLLILFPHAAFASAQGAGESWQLTTQVGGT
                     TQAIFIEGNTLYLGVGMHVEIFDVSNVQQPTLLGSSPILPNFVESIFSDGSNYLYIAC
                     GKGGLQILDITNPASPRLVGSYNTRGYTEDVYLKGKNAILADGPNGIQILDVTTPTNP
                     AWVSEAYPLAYTYDVEMSGNTLFAAAGGSGLLVVDLNDITHPQEKGIVSMDGFLYSLA
                     IADKKMYSANAWGGLGVIDISDPLAPSTLDAIQTDGWVMDVFIQDSNLLAMDGTDGVR
                     LYSIGGQTPQLQGVYADTGFTYQGILQGKNAFVTDKEQGLLILDFTSASNPRLLDQYL
                     PILDARRVTMSGKAAYIAGGLSGMRAIDISDPNNPEETFWFDTEGGYANKVLVEGDVA
                     YLSTHLATEYPLRTFDISDPLHPQLRGQVPNDEAVYQMAFRSMAYSNGYLFIPGEFFD
                     GAVDVRDPANPLVVGRIPLENPINADAYGDLLITVNNNQLQLVDITDAANLTQISTFE
                     RTSAGEGIIFFDETTVITSSDTGIMLVDVSTPSSPREISNLTVAGTVMEIFLDGNTAY
                     LSCLGGGIQIVDLSDLETPVLIGSVETPGIAYDCFVQGNLLLVADSFGGLLIFQRNAT
                     GNTFNNSGSSTAWIQSRDRLNNGANIHTVSSERTVRSFNTEGIHENQKTSLQSSSTCT
                     VTTTADDGSGSLRECISNLREGMTITFDTQIFSPSKPATIQPLSPLPAIEQSTFTIDA
                     SNAGVILDGSQLESGNGLEWYSSNSMVMGLQITNFPENGIRVNGDNNQIGGNRLSGEG
                     PTGEGNLLSGNGINGIILYGNNNVISGNLIGTDVKGTSAFPNYYGVFISEWCKDTIVG
                     GISAGEGNVISGNTFTNLDTWGNHTQVMGNLIGLDITGSKAIKTDSRSNIVIESGASN
                     TVIGGTQPEMRNIISGSDLGIIMSDPNSYQNSVIGNYIGTDITGTKAIPNNTGLTIWT
                     VSFNRVGGTAAGEGNLISGNQIGINLNGYGVTDNIILGNTIGLDASGEKLLEGSVGIS
                     INMGQKHDIIGGYTSAEGNRVAARDIAMRISEPGIEYLYIAGNTISSSGGSGIYFESR
                     SSKNFVQNNSFSQIASNAVRVDYGIDNQLRANTFAVKAENAILLLENGNLNLPAPVVD
                     AATTYSVSGTTCSNCLVELYIIAENTLQYAGETSADANGAFQWAGCELIEGKQVVALT
                     IDAEGNTSIFSSPIVITIDENHEPANCSITE"
     gene            56867..58285
                     /locus_tag="PLL88_01030"
     CDS             56867..58285
                     /locus_tag="PLL88_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HOJ00174.1"
                     /translation="MHKNHFCVHGHFYQPPREDPFTGEIPNEYGAGPFGNWTERIYQE
                     CYLPNAQMGNYQKISFNIGPTLFAWMEKKHPETIRAIADQEKQVFQRTGVSNAMAQPY
                     FHNILPLASEQDKEILIRWGIKDYAKRFGHAPQGMWLPETAMDVVSLEAIAAQGIQFT
                     ILAPWQAKDPLVDTTQPYRVALPSGRELFVFFYNAFLSGEISFNPSSSENADTFVQNW
                     LVPQMDSLEADHEKLVLVASDGELYGHHQRFRERFLSYLLDRAVEKTDIAHQFPALWL
                     RDHAVEQTVELLENTSWSCHHGIERWRNVCGDAPSATWKKPVREFMNGLADAIDKEYE
                     SHLQNLIRDPWKLRDAYVDVLLGEISVTDLIAEHAAQSLTPGQQALAQLLLEAEYERL
                     RMFASDAWFYFDLDRIELLNALKYAAHAAGLTEQATGHNPTETAVHILTQAHSEVSSL
                     SGDIAFLGYLKSFEMKLEQNSH"
     gene            complement(58279..>59987)
                     /locus_tag="PLL88_01035"
     CDS             complement(58279..>59987)
                     /locus_tag="PLL88_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HOJ00175.1"
                     /translation="FWLMEGYFVRTLGMHRVYNSAFMNLLRDEENAKYRQVMKNTLEF
                     DPQILKRFVNFMNNPDEQTAATQFGKGDKYFGICTLMITMPGLPMFGHGQIEGLTEKY
                     GMEYKRAYWDEQPEQSLVDRHTWQVFPLLKKRYLFANVEQFYLYDFFDGNGIVDENVF
                     AYSNRAGQERSLVVYHNRFGDTAGWVRTSVGFMDKRSGTLCQVDLHTGLDLPEGKNTF
                     VIFRDMLSGLQYIRNCHEIAHQGLYIQLDAYRAHVFLDFQIVEDDEEGNWQQVHDALS
                     GRGSQDLRTLQWQLPLRPLLKPLHEIINPRYFDFLAEQRPRVYSESAPESVLNEAVHK
                     LENLIRGAATLLERDLEIEKPALDFKKKLNALFFVEWLDKPRPDLVSQELIDLCDHLR
                     EHTSPYTWLVAICWLYLEGLHNALQVDAAQFFSLVHDWHIFAVLEETLRQSGYLKDAA
                     PDIQSPILFVGAIRDWLRKLNRRSLQGFIAERFADPVIQDFLCVHEYDGKTWFNKERA
                     DAAFLLMTVEGAVEILQTCRAGTKQAQQKLERLVTIVSRLRLAAGSSGYELPRFLELL
                     RQ"
BASE COUNT        16934 a        14776 c        13330 g        14947 t
ORIGIN      
        1 ggtggtcgcc catgatcaat tctcgttgtc cacgaccaag agggatgagc gcatcaatga
       61 tcttggtgcc ggagtacagc ggttcgttca caggcgcccg ctcgatgacg ccgggagcga
      121 tcctttcaac atagcgaaac tcggatggat taatgacccc catctcatcc aagggcttac
      181 ccagcggatc caccaccctg cccaacagtt caaaacccac cgggatgctc aagcgtttcc
      241 cggttgccaa cgcaaggtct cctccgcgaa taccctgatc agaacccaac aagatcacat
      301 ccgcatgcct acgatcaaga ttcaggacca tgccctggac cccattggga aaattcacca
      361 gttcctccag acgaacggta ggcagaccgg agagcgttgc caccccattg ccaatatgct
      421 gtacggtgcc cacgctttga aaatgaacct cgtgtttggt tgcatcgctg tatttcagaa
      481 gtttgtgcag caactgttta acttttgatc gatgatgcgt tgttccattt ttctcgttaa
      541 ttttctgaag agcactttcg acaaattctt gcgcaggttg gttgagcagg cttttttcca
      601 gcagctcgtg tcgaaaagat tccaggatgg cttgttgggc tgaatctcta tcaatcatca
      661 tgttttatat caatttgatc gagggattgt tcgacctcac cgcgcatggc atccaggtgg
      721 gcggcaagag aattatcgat gatcatgtcc gcgatcctta ttttaatccc ggcgatcagg
      781 ttctcatctg tcgcgatatc gagatcgata tccttatccg ccagagcgct gaaggtgcgc
      841 acaagattga gttcttgctg gctattgagg ggcaagggag ttatcacact gaccaccggc
      901 gctcttcctt cgagggattc gcgcagagat tgaacctgca tcatgttggt cttgccgaga
      961 tcccaaatct ttttggtgag ttcttcaatc agagcatcat gcacagcgag cggcgtgacc
     1021 tgtcgcagtg tgtgggtaga gatcgacaag atagcatcaa caagctggga ttgattttgc
     1081 ttcagatcca cagattgttt ctttctgata tccaagaggg cgtcctgcag aatcctttca
     1141 gcttcttctt gggtagcaga aagcagttca gcctggagat tcttattgta ttcataggct
     1201 tcatcggtga ttttctgaat ctcggcatcc aggttgttga ggcgggcctc tatttcagcc
     1261 aaatagcgcg cggcttcttc gcgatcgcgc accatctcat ccatcaagcg ctccttttca
     1321 cgagcacgct cctcaaactg cctgacaatc ggtttaaagg caagaaaata gagtatgacc
     1381 gtcaggatca caaaattgag gatctcaaaa agaatagtca cccaatcaat atcaagcatt
     1441 gattatcccc caaagaagcg agtaatcaac ggattggcaa agagaagcac aagcataacc
     1501 aacagaacgt aaatgttcga ggtttcaata agcgccatcg cgatgatcat ggtcgttctg
     1561 atctggtttg ctgcatcagg ttgcctggca atgctttcca gcgccttcac cgctgcatgt
     1621 ccttccataa gagatggaac gatcatgcct atggcaacgc caatgcctgt caccagaata
     1681 gttactattg taacaagcgt accgggttca agattttcca tttgtaactt ccttccgtcg
     1741 aaaaagtttt tttcttgttt tccttccatc ttcatctgcc tggctgatct ctaccgccga
     1801 agcaatatag agggtcgcca aggtaataaa gatatatgcc tgcacgacac cagtcaccat
     1861 gctcagggta atcagaggca ggggaacaat taacggcaca atggaaaaga taatggcaac
     1921 aatcaaatcg ccgctcatga tattcccaaa caaacgcaaa gcaagcgcaa ccgaacggct
     1981 gatctgacca atcacttcta tcggtaagag aaaaatggga tcagctactg ttttcagata
     2041 cccccacacc ccttttttca acataccaaa ataatggact gagaagaaga cgataagaga
     2101 taaagcgatg gtggtgttga gatcagccgt cggggaggtg agacctggca acagcgacag
     2161 gagattggca gaaataagga agatgaacaa gctacccaaa agagaaagat aaggataaag
     2221 gttatccacg ttcaacacat cacccacgat gttcaaaata aactctatga tggtctcgag
     2281 aatagcgggg ttggtctttt tgatcagaag cacaagcagt acgatcacaa ccatcagaat
     2341 ccaggtcatg acaacggtgc ttcgaacagg aagacccagc aaatagaaag caatcgggtg
     2401 agtcaaaacg ttcatctaag gtttctcgca atcgtcatag ttagcatctt gtttgttttt
     2461 cttcgacaag cgaacaatga gaatgaaacg aacgacggta aacgcagcta ccaataaaag
     2521 ggcatagatg acattcatcc ttatcgcaag gtacatcaat gcggcaagaa acagcatgcg
     2581 aatggcaacc cctgaaagta ataccagaga cgtgccggta ttcgtctttg aagtgatctt
     2641 ctctgcgctc gatttgatcg acagcatgga tagaaaagcg ctacacgcgc caatcacaac
     2701 ccataagatt ccataaccaa ccgttactac attattactc attttttttg ttccctaaat
     2761 cattcttccc attacgaatg aaagaaaatt catcaatgat gaaacgaatc aaactggata
     2821 agccagcgat gagaccaatt ccaagaaata tgagggtata ggttacgcct gtctgcgatc
     2881 gccgatcgat gaagtaaccg atcacaggtc ccaccacaat cggcaacgcc agctcccacc
     2941 cgatagaaga cgccttgaga tctctggcag tgagagcgtg tttccgttta ccacttcgtg
     3001 ggtccatttt ttgctcgtta atcatcatct ccattatctg gtcactaaaa ttttcagctt
     3061 tctatcagct attgcaaaag tagataattc ttgcatcatg taaacaatgt tctttaatgg
     3121 tatttaaaga gccgtggtca ggattttgac tttgttttga taaacagtaa gaatacccgc
     3181 tttgaccggc agatgcgctg gcgtaccctg gcgcacatag cgcagttgac cgtcatccaa
     3241 cgctccgatc aagggcgcat gaccggggcg caagccaatc atcgatccat ccgccaactt
     3301 gaggacgatc gatgtgatcg catcaatatt ggcaacgggt ccgttcacat cgataacttg
     3361 caaagtaaga agatcactgc tcaacctcaa cctcctcacc agccatcatc tgttcagttt
     3421 tttgctgaac atccgcgatc gttccagcca tgtaaaaagc ctgttcaggc aaatcgtcac
     3481 atttgccctc caagatctct ttaaaacctt gcagattttc ttctaacggc acaaagacac
     3541 ccggattgcc cgtatagggt tcggcagaga agaaaggctg tgtcaagaaa cgctgcaggc
     3601 gacgagcgcg gatgaccgtc tcttggtcac tcttgctgag ttcatccata cccaaaatgg
     3661 cgatcaaatc ttgcagttca tcgtagcgag caagggtggc ttttacctgc atggcgatct
     3721 ggtaatgttc attaccgaca aattccggtg acagcaattg agaactggat tgaagcggat
     3781 caatggcggg gtagatgccc agactgacca gacgacgcga gagcacggtg gtggcatcta
     3841 agtgcgagaa ggtggagacc accgccgggt cagtaatatc atccgccgga atgtaaaccg
     3901 cctgcacgga ggtcacactg ccggcgcttg tggttgagat gcgttcttgc agaacgccca
     3961 tctcggattc aagcgtagat tggtagccca tttcgcttgg caaacgtcct aacaaagccg
     4021 agacctcaga acccgcctga atgaagcgaa aaatattatc gatgaacacc agaatagggc
     4081 ggttttcttt atcgcggaaa tactctgcca tagtcagagc cgtgtacgga aggcgcaagc
     4141 gcgcgccagg cggctcattc atctgaccga aaaccaggac ggttgagtcg atcaagccct
     4201 gttcgttgag ctcaagccac agttcatttc cttcacggct acgttcaccc acccccgcaa
     4261 aaacgatgat gccggaatgc ctgcggatgg tgttgcgcat taattcgagc atgaggatgg
     4321 tcttgccaac ccctgctcca ccgaacagac cgatctttcc ccccttggcg tatggcgtga
     4381 gcaaatcgat ggcttttatg cctgttacca ggagctctct tgacactctt tgctgactga
     4441 aagcaggcgg gctggcgtga atagggcgaa gcgtttttgt tttgatcggt ggcttttcat
     4501 caatcacatc accaacgacg ttaaaaaccc gccccagggt ggcaggacca acaggaacct
     4561 taatgggaga attcaggttc tctacgggca agccacgccg cagcccagcg gtgctaccca
     4621 tggcaatggt gcgaacgaca ttggtatcga tgtgctcctg aatctctaaa tacaaatctt
     4681 cttcgtgctt tcgtcggaca agcagagcat tgtgaatact cggcagattc cctgaagcaa
     4741 actccacatc aacaacgacc cccacgatcc tgatgataat accgatatca ttcgtttcga
     4801 aagattgctt attgattatc atgtcctttc atttgtcgaa aatctggcaa aataacaaac
     4861 cccggtacgg gcatgatatt tgttttgatc aaaatagcgt ggaaatttta ccataaaaat
     4921 ggattattcc tgtcaaatat agacgctttc attcttgtga tttgaatttt gggtaatgaa
     4981 aaaatgacag ggtcaagata tgatcaatgg acgaacccgg caccaaacaa aaccagacga
     5041 acccgaaacg gattctcaat cgacagcttc agagttaagc tagttcttac gacccttcac
     5101 agttggggta ggaccccttg ttttttcctg tcattccgaa ggagcacggc gactgaagaa
     5161 tctcgccatt tccctgttga caatctgata agttgcagag caacgagtat ctgaagaaac
     5221 atctttgcga attgttcatg aatcagcttc tatttggtga gatgcttcgc tgacgctctg
     5281 catgacgtta cccttatacg ttatcctgaa aaagttgtag atgcgttggc aacacccctt
     5341 tattctctcc tgtcattccg aaggagcaac gcgactgaag catctcacca tttccgcctt
     5401 gattttccga cagatcacaa agaaatgcat aactgtacgt cattccgaag gagcacagcg
     5461 actgaggaat ctctccattt ctttattaat tctcgataag tcgcaaaaaa agagaatgca
     5521 tccgttctct gttgtttact taaaaagttg gcttcgcttg ggtaagattc ctcgcagagc
     5581 taatgacggt aagaaatggc gcgttgtcaa gctttttctt ctcgcgcaaa atcaggggaa
     5641 aacctgctcc caactccctg aaaacaaaca agaggagtga ttgattcaga agccacgtta
     5701 cggatcaatc aacctctgcc tggcgatgca atttgtgcag aaaatgtcgg atggcgtgga
     5761 gcggatgctt gaagatcatc cttggaccag caaagcgcat gacctgctga atctttgcac
     5821 gatacttcgg agcataacaa tgcacggcgc atcgcgcgca tacggattta tttgcccccc
     5881 agacgcagtg ttccaatcgt tcaagactgt accgcaacaa tgaatcacag tcagaacata
     5941 acccttcttg cgagttatgt ttgccccgac agtatatgcc caccatcact ttaatagtat
     6001 ccatctctct aaggcgataa tcagggacca taaccctccc atactagttt gcagattttc
     6061 ctctaaaaaa gcgcttttcg cacatctccc acgaaagttt ttttattcta ctacagggtc
     6121 cgtttctgct catattttgc tgacgccgga taaccttttt ctttatctgg ttgtaaaatt
     6181 aaacccgtag caaattcgat caaacgaggt tcccatgccg caatacgagt ataaagtgct
     6241 caagatcccg cacgtccagc agacctatta ctgcagcttc atggcttgtt tcggctggca
     6301 ggtgcagaac atccaggaaa gcgtggaccg ggtggtcaac cgctccatgg gtttctccaa
     6361 taccaccaac tacggcagct tcaatgccaa cacctatttt catcctcata ccaacagcgc
     6421 ttccacttac ggctattcgc gcaatcgtgg ctggggttct cagatgaata tgcaggtcac
     6481 cgatgtggag agcagcctga cgatcacctt cttccgtgac gcaaatatcc catacctcgg
     6541 cgagctgaac gatatcgaaa agcgcttctt tgccgccacc cccgcctatc tgcagcgctg
     6601 caaacagaaa gacaggagca aagaaaacca atggccagag cgcatcgcag tgcaaacctg
     6661 tgccgatcag gggaaagccg tgctggcgcg cggaaaacag caggcacaaa ctcaaacgac
     6721 cacacagcaa agggtggaag cacaagccag cacaacaagt caagagcaac cagcatccat
     6781 tccatccgaa ccgcccagcg caaccatccg cgaaacggaa gtgacgcaca acgtattcca
     6841 ggacaaccag cccggcttgc agatccgctt gaatttcaat atcaagaacc gcaaaggcgt
     6901 ccagtgccgg gcggtgacgt atttcttcga tgaaaagcac aacccgctgc aagacgtcaa
     6961 tcagcgtttc caaaccgcaa acggaaaagt atcggtaggg tccaccttta aacccggatt
     7021 tgaggattgt tactacaaca actatatcct gttcctaccc tacggcgaac tggaccagaa
     7081 ggatggagat taccgcctgg gactcaatgc caaaatttat gatgaggtca ccaaagcttt
     7141 tctggcatct tctcccgaca tcttcttcca ctatatacaa aatgggcaga gcatgcgcgg
     7201 ggagagcatc gccacacctg caaccaaaac tgccatccct gaacgaccca aaacacccgc
     7261 acgaacctcg caaaaacccg ctccatcggt caaaccggca gtaaccccgg aacgtcccag
     7321 tttcgaggaa tactccgcca ccttctgcaa gagccgcggc tgggatgttc tcaccgagga
     7381 ccgcaaagtt tttctgcaag gtttggagat ctggtacaag gattttcgct ctgccaaacc
     7441 gctggcgttt cataaaaaag ctttggattt gaacccaaaa gaacctacat actggcagtg
     7501 ggtaagcagc gcatacagtg acaagttgaa gttcgatgaa gcgatcgctc ttttacagaa
     7561 agggttgaaa gaaatttcca atgatcctgt actgctcacc tcgctcggtt acgcgcatat
     7621 tcgaaaaatg gatctggatt cagcgcagaa gaccgctgat atcctgtcaa ctctggaaga
     7681 aagatcggca aaatacagct acaactggct gctgggcatg atcgccgaga cgcgcaaaga
     7741 tttcaaaacc gccatccagt attatgacaa agcagatgaa ttgtccgatc cgggcaattc
     7801 tacgtttctg ggcatgaacc aaaaacgctg ccgtgagctg atgaaacaga agaattaatc
     7861 tggtcgatga ccgaaatttg gcgccgggtt caatatccta ctatcacctc tacaagaagg
     7921 gaatgctctt cttaatctgc gcctgttgaa aaatccatca accccattgc gccaggcatt
     7981 ccattttcag taaagaagca acggtgccag aagattaccg ggaaggtaaa cttgcctata
     8041 cgctcatcaa gcgaagataa tcaggcagaa tatcagaagt gatgtaacga gatgcttctt
     8101 cagccgaaat ggggatgtta ttttccaacc accacaccgc aatcccggta aaacccgcgc
     8161 aaaagaaacg caaattcatc tcaaggcgcg ggtcggaggg ggaatctgtt ttctgcatgg
     8221 tcataggctc aaagatcagt tcagataata actcttggaa taattctcga aagaacggat
     8281 caccgaactt tccgagcatg gagcggtaaa atctctcgta ccgttgcaaa tgttgaaaga
     8341 cactctgcac aaaaggcacc agagaagctg catcattttt ttgagaatcc gttttatggg
     8401 catagatctc tgcagtcatt tgttcaaata acatgtgggc gcaatcctcg agcaactcat
     8461 gcagccctga ataatgcagg taaaaagtag tacggttgat ccccgctccg ttcgtgacat
     8521 ctttgatgcg taggctgtca taagcttttt cagccgcaag cctgatcaag gctgctctca
     8581 ataaattacg agtccggatg gaacgcggat cagattcaga cacattcatt tttggcattt
     8641 ccttgataga caaaaccagc atggacgttg agaaaaataa tttcattttc attataatcc
     8701 agataatcga cttgatgtcg aacaacggtt gttgaaagag aaatccaaaa tgaagaccat
     8761 ccttgactac atttttctcg aacccttccc cggtgactgg ttgatgcagg acatcatcgc
     8821 gatactcttg acctttttgg tgctggtgtt catcgttcgc cgtgaaaaac atgcggcaat
     8881 tgtgattttg gagatggctg cattcgtatt tctatacgcg tctatatacg aaaacgctgc
     8941 cattgtcatg ggcttatatt cttatggtcg ttcgctggtg atgatcggtt ttgtgccagc
     9001 ctctgtgccg ttaatcgaag cctgtgtgtt gatcactggg ctctggtttc ttgaaaaaac
     9061 gcgggtaccc caatgggcat gggcaccgat catcgggctg ttcggaatgt tgcaagattt
     9121 ttcgcttgat ccactggcga tacgacaaat acacacggtt ggtgcagtga cctccggacg
     9181 ctggaattgg ttgatcaatc ccgcaagcga tgccaacatc ttacgcatac cggttttcaa
     9241 ttttccgggt tggatgttga taatgttcta cagtacgatc tgcttgctca tcggacgctg
     9301 gtggtataag aaatctggct accgcccgct ggtcggctat atctatccat tgattactat
     9361 ggtagccgca ttacttttga tgatcagccc gctctcaaat ttcctgctct ggctgggacc
     9421 tttctttcag aaaggacaat ccattgaatg ggtaatgctt gcattccatc ttgtgattcc
     9481 cagcgcactt ctgattttct tatggcgggg aaaaatgacc agtcgcttca ctgcaaatga
     9541 tctccccata ttcatcgttc ccactgtact gcatctttca gatatactgt tcactgtgct
     9601 tgggggatac acagaaattt tgtggatcgt actgctcgcc agtgcgctgc aaaccgcatt
     9661 cttgctgttt gcatgggtca acaatcgcca cgcaccgata acagaaaacc aatagatcga
     9721 tctggcgaga acttcaacat tggagaaaat ttcagcgacc catcaatgac agaggctagt
     9781 tcaagagtca gtatcttcag gtcgtgctgc gctcttcacc tggagatgct gactctcttg
     9841 catgcgtgct gagaaacata cggcgccccc tggcaaccgt ttgccaatca tctctttccc
     9901 acaggtatgg ggtttttcca aaagaaagtg taaaatctat agggaaggat gaaatggctt
     9961 gccgcatttt aatgtaagca aatctgcgac ctccaaaatc agaatttctc aaatcaatct
    10021 tgaaaaaggt taatattaaa atatgaagac gcttcagaat tatgtccaag attttttcgc
    10081 acttctcaat tcaacaaggg agacagaagc ggaacagcgt ttcaccaacg ccatcaagat
    10141 ggcactgctc acctttctag acgatgagag caaggaaaat gcggcgacgg tatacgacat
    10201 cttcttcagc acctaccgca tcgtcctgga gggttccaaa agcaccttca tcgatttgct
    10261 ggatgtattg agcgcctacg aagaacgggc tgccacactg atcgataaac aacgtgacca
    10321 ttacatccat tccgtgaacg ttttcatttt ggggctgtgc atctattcgc aaaactccaa
    10381 ctttcgcaca gcttttaaga aagccaatat cgatggagat aaataccctg aaaattatcc
    10441 gaacgaacag gaagaatttc tgttccgctg gggaattgcg gcacttttcc acgacgtcgg
    10501 atacccaatc gagatcatcg gcaagcagat cgagagcttt ctggaatttg ccaccaacgc
    10561 tgatcatgct gacagcaagg ggaagatcaa agcgcacctg gaattccaaa atttccggca
    10621 cctgaattcg gtggcagagg tgactccaaa aaaggttttc atcaaagact tttacgagca
    10681 aaatgaaagc agcgtttata tcgatctttt acaacccatc gacctgctgg cacaaaagat
    10741 ccatctgtct ttggctattc cactggacga gatcaagaac aaactggact cgttcgtgct
    10801 cgacatggct aaattcggct tcatcgatca tggtttttat agtgccatca tcgtgttgaa
    10861 atggtacggt tatctgatcc aaaagaccaa tatgaacccc atgcgttttt ataatgccgt
    10921 cgtcgacagc gccagcgcca ttctgttgca caattattat cgcaacgcat gtcagaaaag
    10981 cttcggctgc ggtccgatga acccggtcaa ccaccccatc gcctacctgc tgatgttctg
    11041 cgatgagatg caggattgga accgggcagg ctacgggaag attgaaaaga cgcgcacaca
    11101 agccgcttcg gcaaagatct tcatcgatga aaagaacttt gggatcacct acctggcagg
    11161 caagggcact ttttccagga agttcatcga tgacaagaac gagctattca atcaactgtt
    11221 ggatattcgg gcggttttcc cgggtgggtt gcacatcgaa tgtgatacgc tggaggttat
    11281 cttcaaagaa gcccgagaag acgttctggt gccacgcccc atgctggaaa acatggaact
    11341 gctggcaaaa gccatccatg aagactacat cgcagcgcgc aagaagagaa atgagcccat
    11401 acgggtggcg gaaaagtttg aggatctgga cgattcggca aggtattcga atttgcgcca
    11461 ggcgatgaac atcggcaaga agctgcagaa ggtcggctat gtactggtca gccaggatgc
    11521 cgatgggcag gagatcaccc agttggatga tgacaccatt gaacaatacg cgattctgga
    11581 acatgacgac tggatggaag gaaaaatcaa agacgggtgg agctacgcct ccacgcgcga
    11641 tgacagccag aaaaaacaca actgcctggt tccctggagt cagttgtcgg aaatgtataa
    11701 agaatatgat cgggatgtag ccagaaatgt gatcagattg gctgcgatgg ttgatatgaa
    11761 agtgattaaa gcttaattat gacagaatca tcgaagcaag gagaagccat gaaagatccc
    11821 actataccta gcagttcaag cgaaagcaaa tccaatctgc ccttcgcatc ttacaaaggt
    11881 gatggaccgt atatgttcat cagctattcc cacaaagaca gcggagccgt ctacccgatc
    11941 atcgcacagt tccacggaca gagatacaac gtatggtacg atgagggcat cgagccgggc
    12001 atcgaatggc cagaagagat cgcaaacgcg ctcaacgcca gcaaactgtt cgtggtgttc
    12061 atttcaccca actcggtggc atcggagaac gtgcgcaatg agatcaactt cgcactggca
    12121 aaaaaactgc ctttcatcgc catctacctg tgcgaaaccg cgctgacccc cgggctgcaa
    12181 ctgcaaatcg gcaccaagca agacattcga aaataccttt tggatgaaga tacgttcaga
    12241 agaaaatact taaattcttt cgaaagcgtc ttcaaaaagc aggagacgaa accgtgggtc
    12301 ccggaaccca aaccacccga agcggaacgc gggaaaccgg accctgtcat caagaccccc
    12361 acaaccatca ccaccccaac catgccaccg gctggggcgt tgcatttcac ctgtgatgtg
    12421 atacgccggg cagcttgcga gaaactaggc ttacccgaag acaggcagtt ggagctcaaa
    12481 cataccaatg tggtcactga actgcgcttc ctggctgatg catacggcga accatatctg
    12541 gcatgttcca agggtaaaga gcgcttaatg gtatataaaa aggaaggtca accggtgttc
    12601 tttttcgagc gtggtagcat tgcagacctt tctgacctgg cttattttaa aaatctcaaa
    12661 gacatccgtt tgatctttca tcgcttctct gatctgatgc cgctggcaag tcttccaatc
    12721 aagatacttg acctatcctg caaccaactg gctgacctcg cccccctggg cgcgatgccc
    12781 cagctcacca acctgagctt ggattattgc acttttgaaa gcctgacgcc cctaggcaat
    12841 atcaagacac tcagctttct atccatggac gagctttcgt atcaaggatt caaggaatta
    12901 tgtgctcttg acctgccgaa tctgcagaca ctgcacattc cgttcagcaa attggaaagt
    12961 atggagggca tttccaatct gaaattcctc acctggctca gcatcaacga ttccatcatc
    13021 tttgactttg acgagatcgc aaaattgaag aacctgacca acctggcgat ctccaggaca
    13081 aagtgctaca atttttcttt cttaaaggaa ttaccacttc tgaacacgct cacagtagat
    13141 gaggaacaaa aggcacagat catcgaacta tacggagaac aacccgcatt tatcaaatag
    13201 agagcaaagc gttggttaaa gaaaaaacaa aacggttgta aaagcaattt ttcaataaac
    13261 cccaatcgaa aggcagaaaa gtatgaaaca gcacaacaag cacctcaaat ttatcctcat
    13321 tcccattctc ttcatcacta ctcttatctt gaacgcctgc gcaagcgagc cacaggaatg
    13381 tgtgtgtgtg tgtcaggcag gagaaactgc agccggcact gatgcaacaa attccgaaga
    13441 gccggtagaa aatgccgctg ccgcaattcc cgcagagggc ggcgaattcg tcccctgccc
    13501 catcgctttt gattccgatc gcgacggcaa ccttgagatc tatacaatgg actccaacgg
    13561 ggagaacctg gttaatctga ccaatgaccc ggcagatgat tggaatcccg actggtcgcc
    13621 ggatggcagc cagatcgctt ttgtctctga acgcaataat gaacgatcta tttacatcat
    13681 ggatgctgat ggaagtaatt tgcgccagtt aaccaacatg ccggaaagcg atcttgctga
    13741 ttggtcaccg gatggaaaaa ccatcgcttt cacaagtaag gcaaacggga attttgagat
    13801 ctatatcata aatgccgatg gcagcaatga acccatcaac cttaccaaca acgatgctca
    13861 ggatctcgta ccggtatggt caccggatgg cagtcagatc gcctggctga gcggtgatac
    13921 tgaaggttgg gatctatttg taatgacctc agacggcagc aatgtacgcc agctcaccga
    13981 tcagggacat gtatataacg tcaaatggtc tatcgatgga aatctctatc ttgactggga
    14041 aaataatgtc tacggctctt tcaactgcct gatggatcca gacggagaga tcctcgccag
    14101 tggctttgga aaagggggct tcgtgcaata cttcccatat tggacactgg aaggggataa
    14161 ggttgaactg gtctcagcag atatggatgc tggcaataac gaaatctacc tgatcgggga
    14221 tatcttccct gattttattc tcaacatcac caacaatccg gggaatgacg aaaatccgga
    14281 ttggccagca ttatgcggac tgagatagag aataaatagt tgaccgaaaa aagagtgcgc
    14341 ttgcagcgcg ctcttttttc atgattgcat cacggaaaat agaacacccc ttgatataat
    14401 cacttggtat gccgaaaaaa ttatttaccg ctcttctgat cggcgcaggc aaccgcggaa
    14461 ctgagatcta cagcgcgtgg gtactggctc atcccgacaa attgcgcatc aaggcggtgg
    14521 cggaaccgat cgaagccaga agaatggcga tcgccagcaa acacatcgtt cccaccacca
    14581 catccatcat tccccgcacc gtcgtctttg attccgatcg tgatggaaac cgggaaatct
    14641 atatcatgga ttccaacggg gaaaacctga taaatctgac caacgatccg gcagatgatt
    14701 ggacccccgg ctggtccccg gatggcagcc agatcgcatc taccttcatt cgtggtatgg
    14761 gacagggaaa agcgcaaaat atcttgtgcc tgtaatgctg ctgcagcaat agcaacatgt
    14821 tcaccgaatt ctttgtcatt atcacaggtg atgtgtgggt ttgatgcatg aaagggcagt
    14881 aaacctgtaa tggcttttct aatcgtcttg gcaatgcgat cgtccacctt ttttctctct
    14941 tgatgtcata tcacgacgtt agggttgtgc ctttagagtg cagtctatgg tacacacaaa
    15001 ttaagaaatg cttacaaaac acaaaaatag attgttgtca ctatacctgc catagattta
    15061 tatagatggt acccaattga ctatgaaaca cgtgcgagca ttttgtgttc ctttattcca
    15121 ttgaccaata aaacaattat taccgcaaag tatggcattg tcgacaataa ttgagtaggt
    15181 aactcaaaag aagtcaaagt caggaataaa gcatctgtat aagcaaacaa taatgcaata
    15241 agaccggctt taatgggatt gccctgccca acaagtacag cagccaagca aagaaaacct
    15301 cgtccagaag tcatgttctc tgtaaacatt gaaacacctg ataatggtat ataggatcca
    15361 gccaaccctg tcaaaacact cataataaga agagtcaccc atttatactt taacaaattt
    15421 acaccagccg tttcagcagc ggttggattt atgccaacac cccgcacccg tagcccaaat
    15481 gttgttttat acatgacaat ataaccaata aacataatca catatgaaag atacaccaaa
    15541 atgattttgt cattaaagac cgtagtaata aatggcaaac catctaaaaa cggaagatga
    15601 atttgatcat aactgataat tttttcgctt ataaaagctc cacgagcccc aaatatggat
    15661 tgaagcagca aagtagttat agcccaagca ccaaagttca aagcaatgct aataattaat
    15721 gcatctgcct tatagtgaaa aaccaacatt ccaaagataa tccccataac tatacaggca
    15781 ataattccga tcaatgatcc ccctattgca cttcctgtca tatagctgcc ccaggttgca
    15841 aaaaatgcag aggttaacat aaaacattca tatgctatga tgaatgtacc agccctattc
    15901 gtaaaacatc cccccacagc acctatgatt aatggggtag caatacgaat ggtaccagca
    15961 aaaacaattg gactgatcat cgcaagtaag atatctgaca tcattagcct ccaacggatc
    16021 cttttttgaa aatactctga atctccttaa aaagaacttt atagtcaacc gaaacaaata
    16081 atacgaacag cattagtagg atattgacag tcaatttatt caatgatgtt aaacgttcca
    16141 tatttagaga tcctgcttta agcgctcccc acagaaatga aacaagaaca atcgcaattg
    16201 ggttcttgtt cgcaatgaaa gcaatcatta ttccatccca gcccatgttc gacgcaaatt
    16261 gtgctgtgaa tctaaaataa gaccccaata cctcaacccc accactaata cccgcgataa
    16321 atccggaaag caaaaaaatc gaaacaaaac tgcttttcgc attcacccca ccgatctttg
    16381 caaattttag attttctcca acctgtttta attcataacc ctttatcgta tatttataaa
    16441 aaattgctac aaccaaagca ataagaacag caatgataat tccgtaactc gccgatgtgc
    16501 ctttaatgag attaggcaaa acagcggaat cgtatatttt aggtgtggca attgcatttg
    16561 atcccaagtt ggatactcca tgaccaatcc accacatagt tatatactcc gttaatagct
    16621 tcgccataaa atttaacatc aaagttgtaa ttaattcact cacattaaag aaaagtttga
    16681 gaattgcagg gaagaaagcg tacattaatc cagccagacc ggcagctaat agacacaaaa
    16741 atatatgaat tatggggggg aatttatatg aataccctac tacggcagca actaaggcgc
    16801 caaaatataa ttgaccctcc acccccatat tgaatatccc ggctttaaat gctattgatg
    16861 ctgcaattcc tacaagaata cagggcattg cataccgaag agaacttcca aagctgattt
    16921 ttgttccaag tgctccctta tatatctctc taaatgcatt aaaagggttc tccccttgga
    16981 gcaatatcaa catggaacca attacgataa ctgcaagaaa tgccgctata tatttaatga
    17041 cactattctt aatctcatcg tttattttta aggaacgttt cataaaccga ttctcctcca
    17101 gtcattaata atccaatttc gttttcgctt aattcaccat gtaaaccacc ctctttaagt
    17161 agaccgttat acatcacgaa aatacgatcc gataattgca ttacctcatt caagtcatgg
    17221 cttatcaata atatggctac tcctgtagca gcaatttcta ttatcttttt ccagacaaat
    17281 tctatagctc caacatctaa accgcgtgtg ggatcttcga ttacaagcag cttatatccc
    17341 tgcaaaaact ctctgcccac aatcaatttc tggatattcc ctccagacag agattgaact
    17401 ttatcatcaa tactctgtac ctttacgcta tatttatcga tgatttgatt tgtaaatgag
    17461 ctaacttctt tcctgtcaag aaaaatcttg ctcttaaaac catgaactat atggaaaccc
    17521 attatggcat tttcccatat cgatgaaaga gtatttatcc cttcattcat tcgatcctga
    17581 gggatatagc ccaagcccat taatcgtctt tcacggattg atttttcaac aacattttcc
    17641 ccgaaaagaa aaatcctgct acccttctca ggtttgagaa gtcccattac cgtttcaatc
    17701 agtttttgtt gaccggaacc tgctatccca gctattccaa caatctctcc agttctaagg
    17761 tcgaaattga tatctcttaa ccgataacta ccattatcaa tgtatgaaag gtcttttact
    17821 tccagaactt tttcaccaaa aacactgtct aatttttgtg ctttaagaat gacttctctt
    17881 ccaaccatta aactagccag ctttgcttca ttaatttctg atggcaagat atttcccgaa
    17941 acctttccat ctctgagaac ggttatataa tcggaaatag caaatacttc cttcaattta
    18001 tgggtgatga agataatggt tttaccaata ttttttaaaa attttaaagc agaaaataat
    18061 ccctcaatac ccagcggtgt caatactgac gtcggttcat ctaaaattaa taaatcagca
    18121 ccgcgataaa ggagtttaac aatctcaatc tgctggagta aagcaaccgg cagattatta
    18181 atcatgacat ctaaaggtac attaaattta tattgattac aaatctcctt aatgtttttc
    18241 ctgcactcct ctttatcaat aaaaatttta ttgctcccac cttcatagcc cataataata
    18301 ttctctagta ctgataaatc attaaaaagc ataaattcct gatgaaccat accaatgcct
    18361 tgcttacttg cctccatcgg atttttgaaa tttatctttt gccccttgta aataatctct
    18421 ccactatctg gcttgtgcag gcaagtaaca atattcataa gcgttgattt accagcccca
    18481 ttctcgccca caattgaatg aatggttccc ttttttatct tgagatcaat attatcattc
    18541 gctaccaaag agccgaatgt ttttcgtatt tttctcaatt caagaatata ttcatcatct
    18601 ttcataaaat aattatccaa cctttcattt actcgggtta aaaatagcag acgatactgg
    18661 ataattcatt acataattaa cttgtaaaca tgattccgac ttgcataact gtttttgata
    18721 ccagttatgc aagtccaaca ggggggcaca tccccccctg ttgctttaag agactactat
    18781 taaaaattgg tcagcttagc gtctagtagt caagtcgata ttcctcaaaa ccctcgtaaa
    18841 catcaacttc accagtcctg atatcctcaa caagctgcaa aacttccgct tgtagttccg
    18901 gtgataattt ttcaaagtcc ctttcatcat aaacacgccc accagagccg atattgaaat
    18961 ccaggtaaag cgggaattca tacccttcag cccacttact agcaagagtt atgacagcat
    19021 tatccattgg ttttattgca gaccagaaaa cacttggatc caggttgccc tgccaatcat
    19081 caactcctat acaccttatt ccaccattag cgcatgcttc gatcacacca gggcctgcaa
    19141 gataacacga ctgatagata atgtctactg gataattagc tatcatcgcc gctccagctt
    19201 ccaatccttt attggggtca aggtaatctc cagtgaatga atacacaacc tcaacatcag
    19261 gattcgcata agcagcacct gcaatatacc cgtccctgaa tctccggatt ttcagaatat
    19321 ctgtatgtcc aatccaacct atggtccctg tctcagtagt tttagcggcg acaaaacccg
    19381 ccacaaatga cgactcaaat ggatcaacag aaacagaagt gcagtttggt ttgccatgtt
    19441 ccatataggt atccaataag aagagacgag tttctggata taactcataa atctcatctg
    19501 ccaggtctac agcaacgttg ctcaccgcat ctgcaaaagt cattattaca ctataaccgt
    19561 ccccggccaa ggcgcgaata ctctcagcat attctgcact gtcgagcgct tcaatgacct
    19621 ttacttccca gccttcggat tcaagtgcct caaagccact ccaaatcaag tcagtaaaag
    19681 gattacctaa aggcgattct gtgataaaag cagccttttt agtaacttct tctacttcct
    19741 cttcctcaac agcctctccg accggttctt cttccacaac ctctgtttca gcagaagtgc
    19801 aagaagcaaa gactgtcaag agaatcaaca gtactataag actcgaacgc tttttcattt
    19861 ttgttcctcc ttctaaagtt ttaagatcct aatctgtcat tatcgaatat ccttctcttc
    19921 cacctccttg aattaatttt ttcatctcta atagatacaa atcatctatt cagaatattt
    19981 tctgttctca tgcaaattca ttctattatc accccacctg gttcgtaaag tatttacaaa
    20041 atagtgccta gtatgtttta acatactata attaagcgaa atggtcatag aaaattattc
    20101 caaggattat taatgaatac caaaaaagat ataaaaaatg accaactcct tgatatttca
    20161 ttattaattg gtgggaagtg gaaactgata attattcgtg agttaatagg aaaaacaatg
    20221 aggtttggtg aactcaagag atcgattggc tctataactc ataaagtatt aacagataat
    20281 ttacaagcat tagagaaaga tgggataata aaaagagtag tttttcctga gtctacatta
    20341 aaagtagaat attcgcttac tccggctggg tataaatttg caccattgca tcgatattgt
    20401 atctcatgct attatgatta cctctttgca cttaatcaag acaaacttga caatgtagaa
    20461 aatgattaca gatcagaatt aaaaacaaat ttctacgagc ctgattccca tctaaaaggc
    20521 aaaatcgaaa gaaaaaatac atatactgag cgcatatcca gaacaaaaga actaataaaa
    20581 aatgccgatc acataattat tggcaccggt tcaggaatcg atagcaataa taacaaaata
    20641 gaccacttct ccaagcgcaa agcccaggaa ctgtttccaa attttttcga ccttggtcta
    20701 caaaatatcg atgagatgat caacgctttt tcaaacatat acccagaaaa tgagaatgaa
    20761 tactgggagt tctgggcaaa atatatatgg gaactcagat acaatcgacc agtatctcaa
    20821 tcacataaat atctagctga tattgttaaa gacaaggatt attatgtaat atcaacaaat
    20881 tcagatggac aacttgaaga atcgagactg tcaaaacaga gaatccattt gcccctcggt
    20941 aattattctt acttacaatg tacattcccc tgtcatgaga tgttatatga atcaaaagag
    21001 tatatagaaa aaatactaaa tcatttagcc agcggagatg agatgaacaa tgccattact
    21061 cctcattgtc cccaatgtgg aaactattta ataccaaacc actatcgcgt taagaccatt
    21121 tctttggttg aatcaaataa tatggcaaca catagtatgt tctgtgactt cataaacaag
    21181 atacgcaacg ataacaatgt gttcctagaa attggttgcg ggctgaggct accaagtatc
    21241 atcagacata gatttgaaaa ctatctgcat gaatttcaca atacctatct gataaggatc
    21301 aatcgaaggt atccagattt agaatttcca aaacacaaag ataagtttat ttcatttgaa
    21361 gaggatacca aattggtatt agaagatcta tcaattgagt aacgaatatc aatcccgcat
    21421 agataatagt tcgcatgaac tatcaaaatg aaaccgttta aattcccagt tgaacactta
    21481 ataagcaaat tatttcagtt ttaactatag ataatattga gaacacttgt gcaacaaatt
    21541 ttccattgat aaaaaaagtg aagaccataa cattctctca agagctatcc aactaatcaa
    21601 gcaagaaaca agggctattt catgcgttgg gatacccttg gaaggcagca gaagtcccta
    21661 gcaacaacca cgctagccgc aatttacctc cacagcaaaa cttatgaata tctccagcaa
    21721 agagctaatc ccgtttacag gtgaatgcgc agcaatatac ccgaaattga attgcgtgag
    21781 gaagcagcaa aggacatccc atcctatctt cggcgctatc gatcgcgaaa taaccgaaac
    21841 atttcacaag aaaataggga tagtggggat ggaagatatg gcaggttgta cctggaatgg
    21901 gtagcagcga agaaagtatc gtcgaattgc aaaagaatct gtgaagaaat ttacagaaaa
    21961 aaaataacag tcaatcagaa ttgcgatttt ctagaagtta ctaataactc aaaagtgctt
    22021 agtttactaa cattttaaag ccctttatac tctttttatt cttgagaatt caatttggag
    22081 aaatcaacaa tgacggaaag agaaaacaag atactgggga gccttctggg cgcagcagtt
    22141 ggagattcta tgggtgctgc aactgaaaca agatcaactg atatgatcat agaaaaattt
    22201 ggtgggctgg ttgtagattt cgttgaacca cccgatgata acctggcaag aggaacagaa
    22261 gcaggagttg ttaccgatga tttctcagtt gcatattaca cagctgaaga aatactcgca
    22321 acaaaaagcc cagtgtcaaa agagatagcg gaaaaagcgc tattacactg gtcgacacac
    22381 ccagagtaca caagatatat aggtcctacc actcaagtat ctatttccaa aataaaaggc
    22441 gatctggaac aaggctttga ggattctcgt tttcaaaact tgttatgcaa taataaacgt
    22501 gctacaaatg gtgccggaat gaaagccggc attatggggc tttttaatcc atgtgattta
    22561 gatagagcca tcgatgatgc cataaccatg tgcctaccca ctcatgataa tacgattgca
    22621 ttatcgggcg gatgcgcaat agccgcagcc acttcaaaag cgatgagttc aaaagtgtca
    22681 tacatggaag taattgaggc aggtatttat ggcgcttccg aaggatataa gaaatcaaaa
    22741 gaagttgctc gtcctgttgc cggtgcagac attgaaaaga aaatccgttt ggctgtcgaa
    22801 ttaggtttga aatatcaaga caactttgaa aaatcgatga gaataatctc tgagataatt
    22861 ggcggcggac tttatgctta tgaagcaatt cccgctgttt ttggacacat agccgcatgc
    22921 aaaggggaaa cgatggaatc aattctgatg ggggtaaatg gcggcgatga taccgatact
    22981 gttgcttgta tgacagggta tattgttggc gcattaaatg gggctgaata tatccctcaa
    23041 aaatatctat ctttaatcaa cggaaaaaat aatttttgcc tcgaaaaaat ggctctcaac
    23101 attgttgaat ttaccaaaga acaggagaag ttatcagcat gagcaattta tatgataaag
    23161 tatttggttg cttattagca gccgcgattg gggatgcaat gggatcacct ctcgaaacaa
    23221 gaccagttta tcttatcaag aaacactatg ggaatggtaa ctttgtctat gactatatga
    23281 aaccactaga tgacacttta ggacatggct tgccaaaagg tttagttact gatgattttt
    23341 cagttgcata tatttcagga aaacacttta ttaaaaacgg ggggcatata acaaaacagg
    23401 ttgcaagtga tgctttagtt gattggaaag agtctgagga tacaaaagta tattatgaaa
    23461 gatggtgtgg accaactaca ggtaaaagca tagccaaatt gtcaggggtc aaaattgatg
    23521 atccgagaga ttatatgttt tgcgagaaca gaactgcaac aaatggcgcg ggaatgaaga
    23581 gctggatagc aggacttttc aatccaggta atatcgaaaa agcaattgat gacgctatca
    23641 ctatgtgtca tgtaactcac aataatccaa ttgcattatc tggtggatgt gcaatagcag
    23701 caacagtatc taaatgtctg ataaaagatg tcacgctaga tgaagtggtt gatgcaggac
    23761 tatatggagc acatgagggg tataaccgca ctctaaggat aacccgtcca tcagccggcg
    23821 caagtgtaga acaaagaatc aaacttgcgg tggaaatagg cttaaagtac tccaatgatt
    23881 ttgaaaaatg tatattagaa atgacagatc taataggaac cgggctcaat gcaaacgaag
    23941 ctgttccgtc cgcattcggc tatcttgttg ctgggggcgg cgacattatg aagacaatat
    24001 atctggcaat aaattcgggg aatgattgtg acacaacagc tataatggcg ggagctatgg
    24061 ttggggcatt ttctggtggg tcagatattg gtccatatca tctaaagttg cttactgaaa
    24121 ccaatccatt tatggatata ccagatctcg caaagaagat tgttgatttg acaatataaa
    24181 cttggtaatt gatcatgttt ttttgattat ttattagaag gttaagcaat gaaaaaatcg
    24241 aataaatatt tagttttcat aagcgttttg catatggatg aatattacta tactgaaaaa
    24301 tggcctcaag tgggtgataa gacaatattg gttccaggag aaagagttcc agggggaagt
    24361 gcttcaaatg ccgcatgtgt aagttccaaa ctaggagcac cagttttttt ctacgatgta
    24421 ttaagcaaga gcaacactaa caaattcttg ttagaagata tgaaaaaata caaagtcaat
    24481 gttgactatg tcaattttgt tgataattta gatgattcta aatgtttaat aatagcaacc
    24541 cctggtgaaa aaactattct tggaataata catccaaaac cttctacacc tctttcagat
    24601 tcccatatac gcttatttaa cagtgcaaaa tatatctata caactctagg attttactat
    24661 tttattccag acgctcacag cacattcaac aatttcaaaa agcaaggagc aaaactagca
    24721 cttgatgttg aatttggggt aagagatcaa aatgacattt caatcttaga gtctgccaat
    24781 atacttttct ttaatgagtt cggcttctca gctaaccgca agaatttatc ggatgaagat
    24841 tatctgaatt atttactggg caaaggggtt gaattgattg tcattacgtt gggcaagaga
    24901 ggatgcaagg taaaaaccaa agatcttgaa tttgaactac cggcttacga gattgaagtc
    24961 attgatacaa acggagctgg agatacattc aacggcgctt ttttgtatgg tcttctcaag
    25021 gattggcaac ttaaaaaaac cacaaatttt gcgttagctg cagctgccta ttgtgtaaca
    25081 aaaaccggac ctcgtggcgg cgctgtacca gaaaaaacaa ttcgagaatt tttgaaacaa
    25141 ttatgataat aaatcaagaa atggcgagaa gaaatgtttg atgtaattgg acttggaaca
    25201 attgcaatgg acatattttt ccatgttgat aacttgccaa ttgaggacgg ctttgcttta
    25261 attcaaaaat caaatttgct tccaggagga agtggaacaa atgtcgttac acaaataagt
    25321 agattatcag gtaagtgcgg ctatattgct caaattggtg atgatgatat aggtatcagc
    25381 attatagaaa gtcttgttaa agaaaacatc gatacggatg gtataagaat taaaaatggt
    25441 gctacatcat tacactgcaa gataatcgtt gatccaaacg ggagaaaatt catattatta
    25501 gaaaaaggaa atgcgttcga caaatgggct ttttccgaga ttaatcaaaa ttatctgttt
    25561 gaagcaaaaa tcttttatac cgatctttta ccgccacaac cagccgtaag gtctctaaaa
    25621 atagctaaga aagctggttt aacgacagta tttaaccttc aaatgggtat tcaacaaatg
    25681 gaattaatgg gaataaaaaa ggaaaccata atcgatgttc tcgaatacgt ggatatcttt
    25741 gccccttgtt caaacggatt cttgcaactg tgtaattcaa gcgagctaga taattgccat
    25801 aagtatatta gccattattt cgatggacta ctgctttgca cattgggaga aaatggatct
    25861 aaagcgtttt ataaagataa cgagccaatt tttattcccg ccatgcatat taaaccagta
    25921 gatacaacgg gtgcaggaga ttcttatctt ggagctttca tttacgcatt cttaatagca
    25981 aataggaaac ttcagcaatc tatgaaattt gccacagctt gtgcagcata tacctgtgaa
    26041 aggatagggg caagatcaag tccaaatttg gaacaagcac tcataatgga aaaaaaattt
    26101 gacaaagaat tagaaattga ataacacaaa taagatgaat caattttcca aaatttttaa
    26161 agataaaaaa ccaataatcg gtatggttca tattcctcca ctaccagggg caccactatt
    26221 caatccaccc atgacatggg atgaaatcat taaatttacg cttcttgatg ctcaagcttt
    26281 aattgaaggt ggggttgatg gtattcaaat tgagaatcag ttcgacattc catacaatct
    26341 gtcaaaaaac ataggagctg aaacaacagc tttcatgaca gcaattacat gcaatatacg
    26401 tttcatttat ccagagtttc ccctaggtat ccacatatta ttaaacgggt gtatacaagc
    26461 tttgtcagtt gcaaaggcag cacatgctga ttggattaga gcatataatg tcgcaaacgc
    26521 ttatatatcc aatagtggat atataggtgc atctggtcca gcattaatgc gttttaggaa
    26581 cacaattaat gcaaacaatg taatgatatt tggtgatttt caagtcaaac atggaagtca
    26641 tgcaataacc gctgatcgat caatagaaga caaagcacac gatatagaaa cttcattatg
    26701 cgatgcagcg atagtaacag ggaatgctac tggttcacca cctgatatct ctaccttgaa
    26761 aaagattaga gaaaagattt ccataccaat gttgattggc agcggtttgt cacaaaagaa
    26821 tatcaggaaa attttgccat ttgctgatgg tgcaatagtt ggaacaagtt tgaagaaaga
    26881 cggtgttatt agcgaacata ttgatattca atcagttcgt gaattaacta aactggctaa
    26941 gtctattgaa tagtatatgc attcaataca tatcgctaac aagtttgaat taaaacaact
    27001 tcatggatta aaagagtgcg cttgcagcgc gctctttttt catgattgca tcgcggaaaa
    27061 tagaacaccc cttgatataa tcacttggta tgccgaaaaa attatttacc gctcttctga
    27121 tcggcgcagg caaccgcgga actgagatct acagcgcgtg ggcactggct catcctgaca
    27181 agttgcgcat caaggcggtg gcggaaccga tcgaagccag aagaatggcg attgccagca
    27241 aacactccat cccggcagag caccagttca caagctggga agaagtatta agcaagggca
    27301 agatcgccga tgccgccatc atctgcacgc aagaccagca gcacaccgct ccagcgctca
    27361 aggcgctgga agccggttat gatgtattgc tggaaaaacc gatggcgcac cgcctggaag
    27421 actgcgtggc gctggtgcag gcggcagagc agcacggcaa actgctgcag atcgcgcacg
    27481 tgctgcgcta caccgatttc ttccagcgtg tctatcaaac cgtaaattcc ggggagctgg
    27541 ggcaaatcat taccgtctcg cacagagaga acgtctccgc ctggcacatg gcgcacagtt
    27601 acgtgcgcgg caactggcgg cgcgcagacc aagccagccc gatgatcctg gcaaaatcat
    27661 gccacgacct ggacattttg tactggatct tcggcagccg tgccaaaaca ctcagttcgg
    27721 tgggcaacct gaagcacttc cgcacggaaa acgcccctgc aggcgcgccc atgcgctgcc
    27781 tggatggttg cccggctgcc gaaagctgcc cgttctacgc tcccaccctg tatatggacc
    27841 tggagcagat ctaccatggg ttggcgaacg cgcagaacaa gggcatacgg ttggcgggca
    27901 ggctgatgca gatcagtccg gaggtagcgc gcgccgcagc cagggtcgtc aagccgctgc
    27961 gccagatcac cgaatatgag ggctggccac gctcggtcat cacggataac cccaacgatc
    28021 cccaaagcat ctatgaagcc ttgcaaagcg gtccgtacgg gcgctgcgtg taccattgcg
    28081 acaatgacgt ggttgatcac cagatcgtgt taatggaact ggagaacggc atctcggtct
    28141 cctttaccat gcacggtcac agttttgaag aaggtagaac tatccgcatc gatgggtcaa
    28201 aagctacggt gttaggcaag ttcggttggg accattcttt catcgaggtg cgcgaacatc
    28261 ggggagccaa agtcaatcgc atcgaactgc ccaacaacgt ggagcagggc gggcacggcg
    28321 ggggagacgc gcaactggtg gagaatttcg tgaaggcttt agggggtgtg cagggtgctg
    28381 cactcactga cgcacgttcg tcgctggaaa gccacctgat ggcgtttgca gcggaacagg
    28441 cgcgcacaaa gggaagcgtc gttgatatgg cggcatttcg cgctaacgcg gaaaagttgg
    28501 cgtaagcaaa acggggttat tcgagctgta cgtcattccg aaagagcaac gcgactgagg
    28561 aatctctcca tttcctcgtt aattttcgga taagtcgcag gggaaaaaga atgcaaccgt
    28621 tctttgtgat ttatttaaaa attgcttttg ctttgacgag accctttact gcgttgtgaa
    28681 cgcacctttg ttctctcctg tcattccgaa ggaacggagt gactgaggaa tctttccatt
    28741 tcccgattga taatacaaga agtcgcaatg acttgccata tagaaatagt ttttctatcg
    28801 ttaactgatt tattttatcc ttggtgcgat ccctcggttt tgctcgaacg acgttctgct
    28861 gtacgtcatc ccgaaggagc atagcgactg aggaatctct ccatttcctc gttaattttc
    28921 caacaggtcg caaagaaatg aataactgta aaattctctt tgcgagctgt tcatagatcg
    28981 tcttcgtttg ggtgagatcc ctcactgcgt tcgggatgac gtataagggt aacgtcattc
    29041 cgaagttgta ggtgcgttag caatgtaccc ttgttctctc ctgtcattcc gaaggaacga
    29101 agtgactgag gaatctctcc atttccatgt tagttttcaa ataggtcgca gagaaaagcg
    29161 tacctgaaga aacatctttg cgatttgttc atggatcaac ttctatttgg tgagattcct
    29221 cgcaaagctc ggaatgacgt aagggggaag aaataggata aggatatccc tatcgttaat
    29281 cgattcgggc tgtatttttg gagaggttcc ccgcctgcgc tcggagtacc ctctctagcc
    29341 cccgtaaagt ggaaatagaa aagagaggca ggtaccccac ctctcttgct tttttacctc
    29401 tcaggagtcc cgtttttacc cgatcgtttg agcaagctga tgatgagaac ggcaagaccc
    29461 acaactacca atacgatcgg tccgaccaga ctggtagcct cttcggtcag gagaacaatg
    29521 gcagcgatgc ccagcaatac cagggctgga tactttgccc aatcggtgaa atatttttct
    29581 ctcagcaacc acaaagcgcc aaaggtaagt cccagcccac ccagaaatat ccccgccagg
    29641 aaaggaacat ggaatattcg ttcgatgagc agaccgacgg caatgctgac aaagacaccc
    29701 gcaggaatga gcgcccacca gttctttttt gcccaaaaat acaccacgaa gaaggggaca
    29761 gccatcagca aaaccaccgc cacgtcaaac caggcgcccg atacgaaaat ccccaacaag
    29821 ggaatcgtgc caaccacagc caatgctata aagggaataa gcgcccattg gcgactgcgg
    29881 tcagtcacgt acacatacaa gaaaggcaac gcaatgctgt aaagcaccag agcgcccacc
    29941 agattgccat tcaggtagcg ttcgaacaac acaattgcgg aaaccacgaa gaaaacccaa
    30001 gaagggataa gagcccaccg tttggactct ggatcgcgga aataggcaac catgaacggc
    30061 gcagcaacgc ccagcagcac cggtgtggca ctgagcacgc gaccgaggcg cgtgccctcc
    30121 aagccgatga gcagggcaat accagccaga atggttgccg gaaacagcca cccccactcc
    30181 tgcccatttt tcagaagata agtggtcagg aaaaaggcag cggcagcccc aaacaccagc
    30241 gcccaaatca ggttggacca accgtagagt tggatcacac cccattcatt caataagaac
    30301 accgatccca gtgcaatcag cacgatcccc cagaaaatgt tcacactgcg agatttcatt
    30361 tcactccttt tcagacgacg cttatcaaac gactatctcc aggaacaatc gcacatcgac
    30421 aacacgaaaa cgttctcaac tcctcagcgg ttaaaagcgt agtgctgcgg aaaaaacccg
    30481 ctgatcccgg aaaatcgaat cttgcgccgg caattcgttt gaaatagcgg cgacaatcag
    30541 ataaaagcta caagccgaaa tacttcaatg cttcaaatac aaggaacgcg ggccagacaa
    30601 gggctttgag gaaacccaga acgccaagcc agaaagtggc agcattgccg atgaaataaa
    30661 tacaagcgcc gatgaagccc aaaccataga ccgcacctga agctccatca tgacggacgg
    30721 tgtgatgttt gcagcattct ttttcgttca aatctttttc gttatccatt tttgctcctt
    30781 ttagaaatga ttcatattac agatatatac gtcatacccc ctagttaggt tgtgcaaaat
    30841 gaataaataa acaaccgaat ttactgtttg agaacaggtt tgatggaatc gatataaaaa
    30901 atgacctttc aaccctctta tcataaagtt gaaaggtctt cagtgcagcg gctgcacata
    30961 ttaatcgaac ggtgacgttg atctttctgc tatcttatta cctgtaaagg acacagagtc
    31021 gtatcatcca cacacttatt cgctcgcgcc aaagtgaatg gtctggatga catcccagaa
    31081 gagcgtaccc gcatcctccc cttcagcagg tgttattgaa accaagtatg taacagggag
    31141 tatacgccca tgagcccagg caaattcaat cggttcattg aaaagataat tcgctacctg
    31201 cccatcatcg gatgatatga ccgttttaca gaaatccata ttaaaccatt ccgtattttc
    31261 gatcacaggt gttccggaat aagctggcac caaccagagc tgggttactc catagcaaac
    31321 cgccacaccc gtttcttgat ctctttctgc tccatccaga gaactaagtg aagcgatatt
    31381 cgcatacatt ttaaaaatca tcttggaacc ggaaggggtt cttagaatag gataactgta
    31441 cgattcatcc agtctataaa ctttgggaaa ttggaatgtt accagcgagg aatatactcc
    31501 gctgtaatcc tgccaatccg aattttgggc atccacgtat tctggaatag taaaggtggt
    31561 ggtattccaa cctgaaaaat ctttttcaag cacacaatca gaataataat catcatccgc
    31621 gaaatcataa gtagcgcggt atgtttcaac cattctgacc aggtaaccgt cctcggcatt
    31681 tacatataga gtcaataccg gatatggttc gatcaatatg cgataagtaa tattcggatc
    31741 tggttcctct ttatacatcg caatcatttc ttcggttatt ggtatctcat attcaaaaac
    31801 ccttacctca tcaatcgtat tttcacccac ataggtataa tttggatctg aaattttaga
    31861 taagttgcta tctaaagatt cgatttccgg ataataggct atataagtag aactaaccgg
    31921 ctttaataac ccggtagcat ccgctgaata gcaggcggat gacatacata gctttgtgtg
    31981 ctttatgtca aaatcaagtg tcgttccata ttcttttaat ctggtttgct tcgtctgatc
    32041 aatatattct gttgaaaagc ttgtatagcg atcgctatca cttggctggg tactgcccga
    32101 acctgtcaca gtgaaggatt ggacttcatc aaatcgctgc agtgcttttt ccaatgctgt
    32161 ttcaggattc ggtggttcaa cggtaggggt cggtacagat gtaggggtat tggctgccga
    32221 gcccgcagat gtgcatgcgg aaaatatgcc aggcaggaaa accagtatta aaaatattct
    32281 taattgataa caatgacttc cgtacttttt ttctttagtc aatctctttt ctaatctcat
    32341 ttctttgaat cctctcttta ctttaatctg ataaggcgag aatcgacttc gacctcattt
    32401 ataaattaaa aagaatgaca gaattgtcat tcatatcaat taccttatat agagatattt
    32461 atcttccgac agaaccggta cagcacactt attgtactca tcgttgaaat tcctatattt
    32521 tatatggatg ccaaatttaa atattcaaga aacaagataa ttatatagta gctattttct
    32581 caaattacta tgagttatca cgctttttaa gcattcagga gtcaattttt ttaatgatag
    32641 tttgtttatt ttctggcaca gtcatgtgca tccccgtgca ggatctcaac cgcatgctgc
    32701 atcaccggca agaacacctc aaggcactcc acagccgctt tcgggctgcc gggcaagttg
    32761 acaatcaggg tcttcccacg tatgccggca atcgcccgcg ataacatggc gcgatctgtc
    32821 ttttccatac tggcttggcg catcgcctca ggaatgccgg gcgtcaggcg ctccaccacg
    32881 gctgtggtgg cttcgggggt cacatcgcgc ggcgaaaaac cggtgccgcc ggtagtgaaa
    32941 acaacatcaa cctcaccaga atccgcccat tgaacaaggg tcgtcttgat cacttccagc
    33001 tcatcgggaa tgattgccat ctgcgtgaca ctggcatgat tttttatggt tgcagcaatc
    33061 gccggaccac tttgatcttc cctttcccct tttgatcctt tatcgctaat ggtcaaaatg
    33121 gcaacatgaa tggatgggaa aatggtgatt tcatctcccg cgttcacact tccctcttta
    33181 atcactttcg caaagatgcc ctcgctgggc atgatgcact ttcccacctg ttcaaagatc
    33241 tggcagtgct ggtggcactc tttgccgatc tgggtgacct ctaaaagcac ttctccgatt
    33301 tttaaccttg taccgacggg cattgtgaac aattcgacac cttcggtggt gatattctct
    33361 gcaaattttc ccggggtcag gtctttaacc cccagggcag tcatcttatc gatactttcc
    33421 cgtgccagca gactcacctg ccggtgccag ttgccggcat gtgcatcacc tacgatccca
    33481 tgatctattt tcaagcacgc ctgcttgatc ggaagtttaa aggtgccttt tttttcactg
    33541 atattgactg caatcacgcg agccatgcac tatcctccga tcctgaccat gtctttttgc
    33601 ggttcaaaac ccaattcaaa acaatgcctg gcgggcttgt tgaaaatggc atctcgaatg
    33661 gtttctttca aggctgcatc ccctgccagt agcgcttcct ttaaggatat ttctgaattg
    33721 ttccccaggc aggggcgcag catgccatcg ctcatcacgc gcacgcgatt gcaggtggaa
    33781 cagaaacgat gatagatagg gctgatgaac cccacccgtc cctgatgccc atcaatggtg
    33841 aaatcacggg atggctgccc cgcatagcgc ggcgcgaccg gcttgaggta gggtctggca
    33901 gcaatgagtt cgtcattgtt gatgaccaaa gaccgatcct gcccgagttc cgccatgggc
    33961 atcaattcaa taattctgac atccagcggc tgatctttgg aaagcgcgat cagatcatct
    34021 acctccccgt cattgacacc tttcaccaga accatgttca atttcaccgg cagaagcccc
    34081 accctcaccg cttcgtcgat gcctgccagc acagcgttca aatctccgcc ggtcatcttg
    34141 cgatattttt gaggatccag cgaatcgatg ctgacattga tgcgtgtcaa gccggcttgc
    34201 ttcaggcttt ctgcctgttc cgccagcatt tgcccgttgc tggtcatggt cagttcgtcc
    34261 acgccagcta tggatgaaag actctcgacc atttccacaa tatctttgcg caacaacggt
    34321 tcgccgccgg tcaaacgaat gcggttgatt cccaggtcca cacaggcttt tgctatacgc
    34381 aaaaaatcga cggaggaaag ctcggcagct ttggggcaaa tgccctcctc ccttcgacaa
    34441 tacacacagc gcaacgtaca acgctcggtc accgacaagc gcaggtagcg aatctctcgt
    34501 ccaaattgat cgatcattcc tcaccccttg cgtttgcatg gataaaatga cccgacttgc
    34561 cgccgtcttt ttccaccagc cgcactgaga tgatctccat ctcccgatcg actgccttgc
    34621 acatatcata aatggtcaaa gcagccacgc tggcggcaca cagggcttcc atttcaacgc
    34681 cggtggtgcc ggtgcacttt gctgtcgcca agatctccac cctgccgggc gtcttgggct
    34741 ggatatccac agaaatatgg gtaagggaga tgggatggca taaagggatc aattcggcag
    34801 tccgcttggc tgccatgatg ccagccacac gagcagtagc aaaaacatcg cctttcttca
    34861 tctcgcctgc caggacgaga ttccatgtag tctctttcat ccttacggcg gcgtgcgcaa
    34921 ctgccatgcg atccgtactg tctttctggc tgacatccac catcgcaacc tcaccgtttt
    34981 tattgatatg ggtcaattcc atcgtttgat tcttcccggg ttctttaaga tatatccacc
    35041 aagcgcgttc aatcgccggg cgaattcttt tcctttgatt attataatca atctacccac
    35101 cgcttctgtt tcataggcat ctgccgcaat cagcaggtcg tattcttcct ctgcaatggg
    35161 gataaaatcc agcccatata ttcgcgcagc cgccagaata cccaatcccg catccgcagt
    35221 gccggcggca atagcagcag caacggcggt atgggtaaat tcctcgcgct catatccatg
    35281 gatcaatgaa gtatcgactc cattttgttt cgccaagaaa tcacataaaa cacgcgtgcc
    35341 gctacctttt tgacggttga catagctcaa atcgaccaga tcgctgaacc ccttgatccc
    35401 tttaggattt ccgcgtgcga ccatcaatcc ctgcacacga cccacacatt ccatcaacac
    35461 caccccgcca tccggaaaat atcttttcac gtaaggaata ttgtaaattc ccgtctcttc
    35521 atccaacaag tgcgtgcctc ccaagtgtgc ttcccctcgc ttgaccgcca taatgccgcc
    35581 catgctgccc acgtgcgaag aggctatcgt gcaacccgga aaatctcgct gcataatatc
    35641 ggtcacttca tccagcaaag gatcatgact gccggagatc acaagggttc gtttgatctc
    35701 ttccaaatca cggagcaagt gaacattgac cttctctcct gcttcatatc cttctaaatt
    35761 ctgcggaacg tcaatgatac catcagcttt gacaaaagaa ctcaccacgc cggctccccg
    35821 atttaaaggt accgcaatca tttttccatt tacccatcca agtctggcac gaatgaattc
    35881 ccgatatttc aggctagaag taagccggcg agagacaagc gcttcgatcg tttcggctct
    35941 ttcaaatgtc cggcaggtca atttttcgat aatggtcttg aaaaccatct ccatgatcat
    36001 gattcctgag acgggatagc cgggtacacc gatgaccggg atgcaataag aaggattttc
    36061 cctcacaacc attgccagca cagccggttt tcccggcttg atggcaattc cgtgcaatat
    36121 caccgtgccg attttctgga tggcatcttt cgtgtaatct tctctgcccg ctgaagaacc
    36181 ggcattgatc atgaccccat cgcattcatt gactgcatct gccagtgcat tttgaatgag
    36241 atctgcgtca tcccgaacga tcggaaagac cctggggatg caaccccatt ccgttaacat
    36301 gccagaaaag atggtggagt tgaattcgac gatatcgccc tcacccggat tttcagtggg
    36361 agcaacaatt tcgtcacccg tgggaatgat gccaatgatc ggtcttttca ccacctccac
    36421 ctgcaaaact cccgccgcca gcatagcgcc catgcaggag ggtgtgatca gcgtgtacga
    36481 tggaatgatc atatctcccg cactgatatc ctccccgatc tggcggatat tttgccaggg
    36541 aactgctgca gcatagagca cataaaattc cccttcttca accacatcct ccaccatgac
    36601 caccgcatca cagcctgccg gcagtggatc tcctgtgtcc acccaaacaa aatctgactc
    36661 tctcaaacga accggggtcg tctcggtagc gccaaatgtc ttacgggaat ccagggcgat
    36721 cccgtccata ccacaggcat taaaatgcgg agcgctgatt tttgcataca cggcgtttgc
    36781 cgaaaccctg ccaagcgcat cctgcgtcag aatcacttcg gtcttgtaat gcaagccatg
    36841 ctccagcaag attttgagat attgctcaac cgcttcatca agcggggtat tcgttagata
    36901 attgaacggc attttttact cctcaaagag gaaaactttg accggctctc ctgctttcag
    36961 cccttcctga tctctggcaa ttcggaagta tccgtctgca tttgcaagcg tcgtgatcaa
    37021 ccctgatttt ccgataatag gatctgccag accatttttg atggaaacgg gtatcagatc
    37081 ttcacgcccc tggttggaag aaaccgtgcg tgcaagcggc atggttcttg cagtttctat
    37141 cccgaccgcg cccagcatgg aaagaaccaa gggtttgaca agcaaaagaa acatcaaata
    37201 tgctgccacc gggtttcctg ggaggccgaa aacggggaca tgatcaattt ttcccacaat
    37261 cgtgggtttg ccaggtttta ttgccacccc atgcacaagg agctctccca atgccgatat
    37321 cacttcaggg atagcatctt tcgttccagc cgatgttcca cccgtcaaca ccaagatttc
    37381 gcattcattg atcgtttgca agacggcatc gcgaagagca tgctgatcat ctatcacgat
    37441 cccagagaac gctgcttctc ctccgcattc cccaacagcc attttcaaca tgggaccgtt
    37501 gacgtcacgg atcttcccca gcggcagcac ggcacgcgca tcgaccagct catcgcctga
    37561 ggagatgatg acgatacgcg gcaatttcat caccggaaca gcgataattc ccatggcagc
    37621 cagcgtgccg atatccttag cgctcaattt ctttcctttt ctgtaaacca gctcgccggc
    37681 ttttccatca tcccccggaa aaatgaggtt gctgcccggc gcaacgggtt ttctgattcc
    37741 tatcgtgcca tcgccaaaat cttccacatc ttccagcatc accactgcgt ctgccccttc
    37801 aggcacttca ccgcctgtgg gaacagaaac gcactgcccc gaatcaagat ttctatcagt
    37861 atgttttccc attatcgctt cgccgaccag tttcaacata gcaggaattg catcggaaca
    37921 accgaagata tcagaacact tcaccgcata tccatccacc gttgcacgag gaaatgccgg
    37981 cacacattcc cgagcaatga tgtcttcagc caaaatccga tcagccgcgt tttcgaaaga
    38041 tatcatttca ggcgcagttc gaatctcgcc gaaatagtct ttcacaagca tttgaacatc
    38101 agcaagcgct tttacagcga gcatggtgtg cctgtacctg aatgcaatcc attcatccgt
    38161 ctaaaaatgt gacctctacc catgtccctt cctccacttc attctgatca cgcggcacca
    38221 ccatgtaccc atctgcgcaa gaaaccgtgg tgatcaagcc tgatttactg aacaccggat
    38281 aagccattcc atccttgaca atcacaggca cgtattcctc gcgtccatga ttggtgatga
    38341 acttgcgcga taagggaagg atcacttttc gatccactac cggcgtgccc aggaatgaat
    38401 ggagaagcgg acgaacaaaa gtgtggaagg tgaagtaagc cgatacgggg ttcccgggta
    38461 cgccaaagat cggtttcccc tggatagccc cgaagatggt tggcttgccc ggtttcaccg
    38521 tgataccatg cacaatcacc tctccgaggt ccgcgacgat tgccgtgagc gtatccctgg
    38581 catccatgga agtgccgcca gaaacaagca aaaggtcgca ctccgtcatg gcctttttga
    38641 tcaaatcgct gataccctgt tggtcatcct tcaaaatacc ataacagtgc ggtttcccgc
    38701 cgctgacctg cacggcatgc tgcaacatag gagtgttcac atcgcggatc atacccgccc
    38761 gtgtcaacag atcagtggcg ggtacaagct catcaccggt ggaaacgatg ccaacccgtg
    38821 gtcttttcat caccggaaca ttcacaatac ccatgacagc caacgtgccg atatcggcga
    38881 ccttcaaacg cttgccggca gcgatgaccc ggtcaccagg ttccatatct tcaccccgaa
    38941 atatcatatt catcttctca cgatatggtt tggtaaagcc aatcaagtcc tctcctaact
    39001 ctcgcgtatc ttccagcatc accatcgttt ccgttccggt gggcacttca cctccggtag
    39061 gcacataagc gcattgtcca ttttctatgt gaaattccgt atgttccccc atcttcgatt
    39121 gcccgaccaa cgctaatcgg gcaggagagg attctgaaca atcagacagg tcacccactt
    39181 ttaccgcata tccatccacg gtagaacgat caaagtgcgg gatatatgcg gaggcgatca
    39241 tctcttccgc caacactcgc cataacgcat cctgaagagg aacggtttca gagtctgtgc
    39301 gcatttttcc aaaggtatgc tggatgatct gtttcacttc agaaacgggt ttggtcttta
    39361 acatctaacg ctctcctcca tcacgatcct gaacattcat cagttttgca aaattttcat
    39421 ccatcacaga acaaaactac ccttccatac gacatttcaa tctctttcaa ttttattcac
    39481 atcagcactg ccttttcctg acgaggcaaa gaagagtcat gctttatgag aaagatattc
    39541 atagcatgta tattataatt gtgaataaag gacaccctcg tggaaatcaa aaagggcgca
    39601 gcgaaccaca acagcaaagc caatcatctt tggagcctgc ttgcagctcc attattattg
    39661 ttcatcctcc ttcccatcgt ctcattattc ctgcgcagtt caatcacaga catcatccat
    39721 aatctcaata atcgccaggt ttcgcaagcc atcacgctca gcctttccac atccattgtt
    39781 tccaccctgc tgaccctgct cttcggcact cctatttcct atctgcttgc cacccgccgc
    39841 tttcgaggtc ataattttat cgatacgatc atcgaccttc ccaccgtgct cccgccatcg
    39901 gttgcagggg tggctttgct tttggctttt ggaagaaacg gcgtcatcgg aggtctttta
    39961 gcgaaagcag ggatcaccat cccttttact tttatcgcag tggtcatggc acaaatgttc
    40021 atcgcttctc ccctgtatgt caaagcagca accatcggtt ttgcccacgt tgacaaacag
    40081 ctaatacagg cagcagaact ggatggagcg aaccgctggc aaatattccg ctatattttc
    40141 atcccgttat ccggaaaatc tttactcagc gggagtgtga tgacctgggc acgagcgcta
    40201 ggcgaattcg gggcaaccat catctttgcc ggaaatttcc caggcagaac acaaaccatg
    40261 cctctggctg tttacatcgg ctttgaaatt tcgatgaatg ttgcactcac cctttcgatc
    40321 atcttaatct gcttttcttt tctcaccctg atcatcgtaa aaggttttct ccatacccgc
    40381 ctggaatctg atgagaccat cgtctcttat tgacccatga atgcatagtg agaacatccc
    40441 tatcccacca caactagata catttctgga aagtctttcc gcacagagcg gttaaatttc
    40501 ttagcacagc tcctctcaag ggaaatgacc acccggagaa atccaggaaa aaaggtcatg
    40561 tgcattttgg gaacaatgct aaaatagata atagttatat tgtgaatact cacaatataa
    40621 ctattaatgg aaccccacaa aagcgggaat ccattcacaa tttttcgaag gaggatacct
    40681 tatgcgctca aaatttttac ccatctgttt gatattcacc ttgctggcaa ctgtagcatg
    40741 ctccacacag gctgccgcgg aaccggctac aaccgacgtt ccaagtgaag cagccactgc
    40801 tgctcctgtg gaagagcgga ccctgaccgt catggctgcg gcatcgttga cggaatcatt
    40861 taccgaactc ggcttaattt ttgaatcgca acatgaaggg gtgaccgtag ctttcaattt
    40921 cgccggctcg caagcattag ccgagcagct cgcccagggt gccgacgtag atgtatttgc
    40981 cagcgccagc aataaataca tgaccgcagc ggtcgatgca ggtcgtgtga cggcagatgc
    41041 atcttctgtt ttcgcttata accggttggt ggtcatctac cccaaagata atcccggggg
    41101 aataacagaa ttaaaagatc tggcaaagga ggacttgaaa gtcgacctgg cagatgcctc
    41161 agtgccagtg gggcaatatg cgcttgactt tcttgacaag gcttctgcag atgcaaactt
    41221 tggttcacaa ttcaaggaag atgtcttgaa caacgtcgtt tcatatgaag aaaatgttaa
    41281 agccgttgtg accaagatct ctcttggcga agccgatgcc gggatcgtgt acgtaaccga
    41341 catcaccgct gacgctgcag aactggttgg tactttggag attcctgacg agctgaacac
    41401 gattgcaacc tacccgatcg cccccatcgc cgacagcaaa aatgctgatc tggcacaggc
    41461 attcgtcgcg tttgtgcttt ccagcgaagg gcaagcgatc cttgcaaaat acaatttcgt
    41521 ttctgttctg tcaaattaga gaagttggtc gcctaaaaaa ttctttttct tagaatcagc
    41581 attaaagaat taccagcagg tgaaaaattc acctgctggt aattttgtat ccgtccatta
    41641 cgcactaccg attgaaaata ccgcaatatg acaacggtat ttccggttga aaacgcattc
    41701 cgagcatgaa ccaccatgtg ctatccttac cctgcatgtt tcgcgaaaga atgttaaact
    41761 tattttgctg aaacagtgca aggcgatcat ttcacattcc aaagagaaac ccatgaaaat
    41821 acacatcatt attgaccatc cagacccagc cagtttcaat caccagatca tgaacacgtt
    41881 catcgaaggg atcgacaaaa acaaacatga gctcgacgtc cttgatctcg atgctgatca
    41941 atttaatccg gtgatgagca aggaagaatt caaggcttac aaccgcggcg agtaccttga
    42001 tccaaaagtg aaagagtacc aacaacgctt gaaatctgca gattatctgg cactgttttt
    42061 tcccatctgg tggatggtca tgcctgcgcg gatgaaaggc tggattgaca aagtgcttct
    42121 tcccggcttt gctttttcca aaggagccaa tccggaaccg ctgctgtcgc acattacatc
    42181 ggcagtgatc tttacgacca cagcgatctc cgatgaaaaa caccgcaagg atttcaacaa
    42241 cgcactggat tgggtgcttt gcaagggaac gctgaatttt attggaataa ggaacacaca
    42301 atggctgaac ttcggagagg caggtattgc cagccccgaa aaatatcaag aatggctggc
    42361 tcacgttctt gatttcgcga aaaacctata gaaaatgccc ctggaagcag gggcattttc
    42421 aaaaacgata aacgattacc agtaagcgcc acgattattg ggttggatat ccgcaaacgt
    42481 ccaacgcagc agaaagatgg ttctccaggg attcttccgg taaatcttcc acatgctcga
    42541 tgatcagttg tttcttgcca tgctcctgca gaattttttc gtaccctgca tgcaactcat
    42601 ccatattggc atacgccaag tgcgggggca agtttacgga aaggattttt tccggaaaga
    42661 actctttggc ttcggtgagg gaaatattgc cgatgggagg gaccgtgaag gagtcaacga
    42721 tatcaatttt cgtctcatgg atctctttag ccagatggcg cagaagtcca tcatgatgca
    42781 cggcaaattt ctttcccgtc cctgccagcg cattcacata aatatcataa atcggcatgc
    42841 aatattctcg ataatatttg ggtgaaataa ggttcgtgat gttatcaatg agcgtgatat
    42901 gctgtgccgg gcagccagca gtgatctcag ccgctttcaa gtggaagcga tgttgaatgt
    42961 cgagatactc cagcagcaga tcaggtttgg ttttgaagtc gatggctgcc ctttccaccg
    43021 atgccaattc gacccaggcg cgttggaagg gagatttatc gaccacagcg atggtagtac
    43081 catcggtgcc caggtcttgc tggtcccctt ccagttcgtc ataattgggc accatatttt
    43141 cgagcatttt ctcgaagaca aaattgatga cacgccagtc ttccacctct ttcacaaaaa
    43201 aagtttgggc agaatgggtg agcacattca ctcccgggtt gatccccaca atggaatcca
    43261 ccgtgccgac cggggtttcg aagatgtgct ggatcatcca cacattgtcc tggcagaaac
    43321 ttttttgtgt atatgcgatg tctttcagga aggggttgat catgtggtcg aaaaagaaca
    43381 tgggtttgta gggtggaata atatgcgtta tgcccatgcc cctggcggtc aggcggcgca
    43441 acatgccacg cgtgggacct ccgcgcaagc cagccgcaaa gaccacatcc actttatcgg
    43501 gttcttcatt attaattaca gccaaatgtc tttcataagg attcatcatt cagtaccacc
    43561 ttcgatcttt tgttctgcag cagcgatgct agcaatcaaa gcagtttttc tgctcttgcg
    43621 cacagctctg ataaccagcg ccaaaatagt tgtaagatag ggcagcatga gggtgaattg
    43681 cgaaggaata cccatattct ggatgcgcaa tgagatacct tcagccagac cgaacaataa
    43741 ggaagtgatc gccatggtaa cggggttaga gccaccgaag atcactgcgg aaagggcaat
    43801 aaaaccgcga cctgccgtca tatcttcgac aaacagtctc aaataaccca gagagaggtg
    43861 ggcgcctgcc agaccgcaca tgacagagca aatcatattg gcaatccagc gaattttatt
    43921 gacccggatg ccggtcgttt ccaacgcttc aggatgttca cccgccgccc ggaaatagaa
    43981 gccataggga gtgcggtaga taagaaacca gaaaacaaca atcataaccc aggatacgta
    44041 cacaaaagcc gaatgattgt tcaagaggtc attgagcaca ggcaccttat ccaggaaagc
    44101 gaggtggata ttggggatac cttcgatgcg gggatcgcta aaggagccac gcacgttgaa
    44161 gaacaagcgc gagaaaaaca ccgtaacctc tgacgccagc aggttgaggg caatccctac
    44221 gacaaagaca tccgctttca atttgatggt aaagagacca aacagcagcc cataaagcag
    44281 ggtggcaagc actgcaaagc aaattccaac cacataactt tcaaaaaaat aactgccgag
    44341 gacggcaaag aaagcgccaa ccaacatgaa gccttccaga gaaatgttca agtcgcccgc
    44401 gctcaagctg accagtccgc ccaaacccgc caggataaag ggggtagcca tgcgcagacc
    44461 agtgccgaga acatccaaga taaaatcgag atccattatt tcgcctcccc atccaccaga
    44521 acgagatgtt tgcgctttct gaaaatgatc gaaacaaact cacgaccggt gatgaacagg
    44581 atcaaagcag ccagcagaac accggtcagg tctgaaggaa cgttggcaac ctgctccatg
    44641 cccagagcac cgttcttgaa aatgctgaac acaaaactca ctgcaacgat ggcaaaagga
    44701 ttgttcttgg caagcatggc gaccaagacc ccatcccaac cgtaaggtcc ggcgatggac
    44761 tcccagtagc ggtaattcga tcctgccaca agcacagcac cggtcagacc tgaaagcaag
    44821 ccactaagcg ccatggcttt catttggtcc cttgcgacat tcacgccacc gtactgcgca
    44881 aagcgcggat tcagaccgat cattttccag cgatacccct gattggtata gttaaaaatc
    44941 cagtaaatga caaccgctgc caccaaccca aagatgatac ccgaacgcag gtcaatactc
    45001 tgaaccagat aaggcagttt ggcagattcc tccaccgggg gcgtagtgcc gctccagcgg
    45061 gtgacatcac ggaatggata atttgccaga taactggtaa acatgaagaa gattgaattg
    45121 agcatgatgg tagagatgac ctcatcgacc ttccatttca ccctgagata gccgggcaca
    45181 gcagctccca gcattcccat tagaacagca gccagaatgc acaaaggaag gtggatgtag
    45241 atcggcaacc cgatcaattt atagccgatc acagccgcag cgatagcgcc caattcaatc
    45301 tgtcccttgc cacccagatt atagatgccc gcattggaag caacggcata acacaaagca
    45361 gaaaggatca aagccatcga acggttcatc agattagcaa tcgcactcac atctcccagc
    45421 gcataagaga acatggcagc atacgcctgc aggggatcct ttccgatgac taccatgatc
    45481 aatgccccaa aaagcatggt ggcaatgaac actccgacaa tggaggttaa ggaatttacg
    45541 gttctttttg agtttttcac agtgtactcc ctgcattcgg agtggtgcgt ttggctccgg
    45601 tcatatacaa accgacctct tccggggtta cctcgtcggg caagaaattg gcaacgacct
    45661 caccctcgta aatcaccaca atacgatcgc tgagcaggaa caattcatcc aggtctgccg
    45721 acacgagcaa aatggcattt cccgccttgc gcgcatccag gatttgctca taaatgaact
    45781 cggaagcgcc aatatccaaa ccgcgggctg gctgcagcac cagcaagcat tggtgttttc
    45841 ccgaaagttc tcgggcaacc accactttct gcaaatttcc accagaaagc gtgccgacca
    45901 acgtatctgg tccctgcgcc acaatgctga acttctcgat caattttttg gaatgattct
    45961 cgatctcctt ccagttcatc acatctggtt tggaaataaa gggcgcacag tcatttcgat
    46021 cgatcaccag gttctctttg atggaggctt tcacactggc tccggtggtc atacggtctt
    46081 cagggattaa gcgaatgccg agcgcccgca aattggagat cggtttgttg gtgatagacg
    46141 acccgttgaa gaacacttcg ccctgatcaa caggggtcaa acctgccagc gcttccatta
    46201 attccgattg cccgttccct tccaccccgg caacgcccac gatctcgccc gcatgcacct
    46261 gaagggacac atctttgaca caaaacgaat gcgctgtttt tcgaacagaa acgttccggg
    46321 tctcgaggac cgtctgtgga gcaagggatg atttctgttt tctaaccgtc caaagctgtt
    46381 cacgacccac aatgagccgg gccagttcgg gttcagttgt atcctgtgtg tcgagggtca
    46441 ccagcgtctc tcctttgcgc atcaccgtca ctttatcaga gagagccagg acctccttca
    46501 atttatggga gataaataat atggtcttat cttttgcaac catatcgcgg cagatacgga
    46561 aaagctcttc tgcctcgaaa ggggtaagta ccgcggtggg ctcatctaga ataaggatct
    46621 cagctccacg atagatggct ttgatgatct cgacacgttg ttgaatacca acggagacat
    46681 cctgggtgag ggattgaagc ggtatggaga ggttgtaata atcgcaaatc tccttgacct
    46741 tcttctctgc ttccctgttg tcaacaaaga caagattctg cctgggttcg taacccagaa
    46801 ttacattctg cagcacggtg aaagagggga ccagcataaa atgctggtga accatgccaa
    46861 ttcccaattt ctttgcttct ctggccgaag tgatagaaac ttctttgtca ttgatataga
    46921 tgctgccatt atccggttgt agaacgccgt acaggatgtt catcaaagtg gatttgcctg
    46981 caccgttttc cccaactatg gcatggatct cacctttctt gacgacaaga ttcgcattgc
    47041 gattggcttg aacgccgcca tcgaacgttt tgctaatatt ttccaagcgg attagcgttt
    47101 cagttgtcat gggatatccg attctctgta ttcaaaagtg gatgtgacca ggagcggtca
    47161 ttcccggtca catccgaaac acaatagtgg ttattgatcg cggaattcag ttacagtgat
    47221 ctcaccattg gcaatctttg cttgaagatc attcacttca gcaacgatat cagccgggac
    47281 aatatcctca gacatggtca gccacatacc gccttctttt acaccccaat tgaaggtgtc
    47341 acctttctgg agttctccat tcaggtacat ttcagtgagg tcatgaatgg aaacatcgca
    47401 ggctttcatg acactggcga tggtgtaatc aggagccagc cagcgctggt caaaaccacc
    47461 gccgatgatc caggtttcgg cgccttcatt agccgcattg aaaccacctt cgccagacag
    47521 accggcatac atgaaattaa tatcggaacc catttccatg agagccagag tggcttcttt
    47581 gcctttggca ggatcaaccc aggagccaac gaagatgtat tccagtgttc cttcgggagc
    47641 aacatacttc aaaccttgct cataacccca gagggcgcgt tgcataacag gcgaatcgac
    47701 gccaccaacg tagccgatga cctggtcttc attgatgaaa ttgacatcgg tgcgggtggt
    47761 cagcaagcca gccagagcgc cagccaggaa ggaaccttca tggtccttat aacccagaga
    47821 gacaacatcg gcagaatcga gaacgccgtc caggtaaatg tagagggtgt cgggattctg
    47881 gggggcaacc tcaagcatgg catcaatggc ttcgaaacca agcacgaaaa cgatatcatc
    47941 ggtcttggct gcattagaca gcaaagtctt gtagttctca taatccagtt tgccttcata
    48001 ggtgtgcgta actgcgccaa aatcttcttc gatggattca ataccaccca gggcgatatc
    48061 atagaaagcg cggtcaccga aggaatcggc aacgaaaaca cctacggtta tcggctcggt
    48121 atcggtagtt gtttcacttt cggcaacggg ttctgctgct tcagcgggtt ctgcggtgct
    48181 acatgcggta aagatcatcg cagcaataag cagcaatcct attaggggta atacatgttt
    48241 tttcattttt tgtgtccttt tcttcttatt cctttatcat taaagtagaa tcagtaaatt
    48301 ggtaaaattt ctaaacgtca cacctccttc ttcaccccta agacttttcg atccggtcta
    48361 tgacatccgc aactttctct gataaccaac tcggttggca tcatgatcgg ctgtcgggga
    48421 actttctgct ttttcatcag agcaagcaac aattttgcac tttcttcacc catcttttcc
    48481 atggggatgg taatgctggt gagggatgga gcggcataat cacacacatc aatgtcgttg
    48541 tagccaacga tggcaacatc ttgcggcatg cggattgcat gttcacgcag agccttcata
    48601 gcaccaatcg ccagcatatc acctgcagca aagatggcag atggcaaaag gtggttttca
    48661 atcaattgga cggttgcccg gtagcccgct tgataggaga actccttgac atcgatcacc
    48721 aagcggtcat ccaagaccag gctgtttttt tccagggctt cacggtaccc ttcataaacg
    48781 gtgtacaacg tgggaataga aagattaccg gtgagcatgg cgatcttccg gtgaccgtgg
    48841 tcaatgaggt gctgggtacc tttgtagccc gccccttttc catccaggag caccgaatac
    48901 cctttcacgt tcgggcgatc cgcaaaaaca agcggaatag gaaaatcata gaacgtccca
    48961 taatcaaaaa aatcggatgg ttctggatct gtgcctaaag gcgaaataat gatcccatcc
    49021 acccgtttgg caatgagcat atcaaagaac gccttaccaa tcgtgttctt ttcgcgcgta
    49081 tcacaggtga tcatcagcac attgtgagca ctggctacct tggctgctcc gcttaggaag
    49141 gaagggtaaa aggggttttg aaggtcaggg acaatgatgc caaaagtatt ggtcggcagc
    49201 atatgggtag aaactgtttt ggaaggatca taatccaata ctattgatcc caaaccctga
    49261 cgggtggaga tcaacttggt ttcttcgagc agtcgatagg cagcgcgcac agtatgcaaa
    49321 ttcacatgca gatgatctgc caaggtgcga acagagggta aaatatctcc agctttgatc
    49381 tcattcacga cgatcaacca gcggatttgg tcggcgatct gagaggcaat tgagcaattt
    49441 tcatttagtc ccacctgcaa tcgctgaaag atgatttcgg aatgattttc catagcgcca
    49501 ttactgatat agtattatat aatactatat cagtaaacaa aagggaacac aatagaaaaa
    49561 cacaatttat tgcaacgagc aaccatttct taaaagtagt aaggagaaga acatgagcac
    49621 atcattagac gcgatcagcc aggggatcat cgaaggggat atgaccaccg ttacagcgct
    49681 tgtggaacag gctctgcagg agctgatcgc accgcacgcc attctagacg ccatgaccaa
    49741 agcaatgggc gaagttggtc ggctcttcga agaaggggaa tatttcgtgc ctgaaatgct
    49801 ggtttctgcg cgcaccatgc agaacgggct ggatgttttg caacccgccc tcacctcggc
    49861 agacacaaaa gccaagggga aagtggtgtt gggcacggtg aaaggcgatc tgcacgatat
    49921 cggcaagaac ctggttgcaa ttttgctcaa gggtgcgggg tatgaggtgg aggatcttgg
    49981 cgccgatgtc gcgccggaag tgttcgttga caaagtcaaa gagaacggaa aatgcgtggt
    50041 ggcactttct gctctactga ccacgaccat gatcaatatg aaaacggtga tcgaggcgat
    50101 gagcgccgct ggtttgcgcc aggaggtgaa ggtcatcgtg ggtggcgcac cggtaaccca
    50161 ggaattcgca acccagattg gcgcagatgg ttatgcagca gatgccaata aagccgtgcg
    50221 gctcgtggac caactggttt caggataatc tatgaagggg tgtcctggag atttcccagc
    50281 gattactcca aaacaatttc gatatgaaca tcgatctatc catgcttgag cccatagtga
    50341 aactcaccaa tcccgatcag gtcggcatgg tggtggatga tgcgcataaa gcagcatctt
    50401 ctttcgccag aatcttcgga gccgatcctg tggaagtgct ggactggccc gcgcctggtt
    50461 tcgacccaga atcttattat tacggtcaac caacgcgctg gagtatgcgc gttggttttt
    50521 tccagattgg caatctgcag tgggagctga ttcaaccact ggaggggaag agcatttttg
    50581 ccgactttct cagcgagcat ggtcccggct tgcaccatgt gcgcttcaca gaaccccatt
    50641 tcgaacaaca ggtggctgct ctgcaggcag ccggcattcg catgatatcg aacggtatag
    50701 gcattcacca ggtttctcgg tgggcgtatt ttgacacgcg ttcgctgctg caagggtact
    50761 tgcttgagct gcgaaagaat taaccgactt ccaggtcctt tttgaccaac gtcttcactc
    50821 tccagtgtgg cgcaaaaaag cgctggcaaa acttctctcc ttttccggtg gcatttcggc
    50881 aaataccttc ccggggttgg attgatgggc tttcataccc gcagatcgtc cgggctcatc
    50941 tgcaatcaac aaccctccac attttgcccg ttttgactcg aaaggtgcga gccagatgct
    51001 ctttcagcca ggtataaaac cttcttcttt tccagcaagc agacatcttt tctgtgaaat
    51061 tcctttgaac gaacctagcc ggaatgtcct ataatgttta atgagcctgt tgatcaggga
    51121 ctcatcgaac gttccgttcg taaataaaat taacaaggag agagatatca tgagcattaa
    51181 agatcaggtg actccagaac agtggaaagc gctttacaac gcgatcagcg ccgcatccac
    51241 ctacgtgtcc actgccagcg gcggcggatt ggaaatgttc caggaagcat tttctgcaag
    51301 caagttcatg atggaatcga gccagaaggc aggcggcagc ggttatggca gcctggtgga
    51361 tgaaatattg gcagtgatga aaggcatgaa cctcaatgat gccaaagcag acgcgcacca
    51421 gtaccaatcc aaagatattg ccgctttacg agcagaagcc aaacagctag ttgtggatgg
    51481 agcagctgcg gcggctgccc tgcccgatgg agatggctac aagcgctggc tggtggaaat
    51541 ggcgcgcaag gtcgccgaga ccaagacagg tggaattctg ggattcggtg gtcaggcggt
    51601 gatcgatgag aaagaggaag cagcaatcgc cgaactaaca gagttactag gaatataaat
    51661 cacagcacgc gcgaatgatc gcgcgtgctg cattatgttg tctctcaaac aaacaagacc
    51721 gcatcttttc cctttttccg aaaaaggcag tgtattgtac gttaaaaccg cgtgttgagg
    51781 aacacaaaag cgccccgggc gggatccggg gaatgcttgc caggcagcaa ctcaaaaaaa
    51841 gacgaggctg gtaaacgcaa ccgacctcgt ctttccattt ctgtgaatga attactctac
    51901 agtgatttcg accagccccc tgacgttctg cttgccggag agatcgggca tgacggagcg
    51961 caatgtgcca tcatcatcaa aagcgacgat acagttggcg caggcagcca gttcttcgct
    52021 ggtgacttca gcggtaaacc catcagaggc gaccagggtg agttttgtat aatcagccac
    52081 accggcagcg gtcagcaggt cacgaatgag aacgccactg tactgggtag tctcgccgtc
    52141 tttattggtg tattccgctt ccacctgggt catagcattc aggtcatcgg cggtccaaga
    52201 cgcttccacc gcaccgctca ggatgagggc aggttccgca acagccacag cctcttcggc
    52261 ggctggctca gcggtctcgg ttgcgcaagc tgaaagcagc aaccccgcga tcaggacact
    52321 gatcagaata atttttttgt tcattttctt cttctccttt tttgttccat gaacaataat
    52381 ctctcggatc cgttttccca ccccaagact ccatcaaaaa acagccggaa aaaaggctgc
    52441 tgccaattgc caaaaccatt cagcacatcc tctttccaag cgggaggcgg gagcgtttcc
    52501 actcgcaccc tatcggtcta ttgaccttag ccgcattgcc ttaggtcaac aaactcgaag
    52561 atgctatgta atattttagc acagagataa accagagaga cgggtaggat cgatcgctca
    52621 gatcatatgc acagaccgca acatgctcgc cgcccatcgc tatctacaaa cgcagattgg
    52681 caaggtcgaa gtcgctgacc cgcaagtggc agtactttcc actggaacgc cagccggtgc
    52741 tttccacgcc caaaaatgtc gtttcgcaga tttcgcttca caggacaagc atggtgagat
    52801 gcaatgggca ggaagagcaa tgcctgcttt cctcgcctgt caatgaccat tgtgatcatt
    52861 gccaagagct ttcgggcgcc tgctataatt tagtttggat gatgaacaat tcacgtagca
    52921 gcgccgcata taacagccag gaggattaga tgaaaaaacg acatctttcc agattctgct
    52981 ttctagccac atttctattg attttgtttc cccatgcagc attcgcaagc gctcagggcg
    53041 caggcgaatc atggcaattg accactcaag tgggggggac aacccaggcg attttcattg
    53101 aaggcaatac gctctacctg ggggtgggca tgcacgtcga gatctttgat gtatccaatg
    53161 ttcaacagcc aacactgctc ggcagcagtc ccatcttgcc taactttgtg gaaagcattt
    53221 tcagcgatgg ctccaattat ctttacatcg catgtggaaa gggcgggctg cagatactgg
    53281 atatcaccaa tccagcttct cccaggctgg tcggttcata caatacacgg gggtacaccg
    53341 aagatgttta tctcaaaggg aaaaatgcca ttcttgcaga tggcccaaac ggaattcaaa
    53401 tcctggatgt taccacacct accaatccgg cgtgggttag cgaagcctat ccgctggcgt
    53461 atacctatga cgtggaaatg agcgggaaca cgctcttcgc agccgctggc ggcagtggtc
    53521 tgctggtggt tgacctgaac gatatcactc acccgcagga aaaaggcatc gtcagcatgg
    53581 atggctttct ttattcatta gccattgcag ataaaaagat gtacagcgcc aatgcctggg
    53641 gagggctggg ggtgatcgac atcagcgacc ctctcgcacc cagcacattg gatgcgatcc
    53701 aaaccgacgg atgggtgatg gacgtgttta tacaggattc taatctgctg gctatggacg
    53761 ggacggacgg tgtgcgcctg tacagcatcg gtgggcaaac gccacaattg cagggggtgt
    53821 acgcggacac cggtttcacc tatcaaggca ttttgcaggg taagaacgct ttcgtaacag
    53881 acaaagagca aggattgttg atactggact tcaccagcgc cagcaaccca cgcttactcg
    53941 atcaatacct gccgattctg gatgcgcggc gggtgaccat gagcggaaag gctgcctata
    54001 tagccggcgg actatccgga atgcgtgcca ttgacatcag cgatcccaac aaccctgaag
    54061 aaaccttctg gttcgacacc gaaggcggtt acgccaataa agtgttggtg gagggagatg
    54121 tggcttatct atccacccat ctcgctacgg aatacccatt gcgcaccttc gatatcagcg
    54181 atccgctcca cccgcagttg cgcggtcagg tgccgaacga cgaagcggtg tatcaaatgg
    54241 ctttccgttc catggcttat tccaatggat atctcttcat ccctggcgag ttctttgatg
    54301 gcgccgtgga tgtgcgagat ccagccaacc cgctagtggt cggacgaatc cctcttgaaa
    54361 accccatcaa cgctgacgcg tatggtgatt tattgatcac ggtgaacaac aatcagctac
    54421 agcttgtaga tatcacggat gcggcgaacc tgacgcaaat ttcgaccttt gagcgcacca
    54481 gcgcaggaga agggatcatc ttcttcgatg agaccacggt gatcacctcc agcgacaccg
    54541 gcatcatgct ggtggatgtc agcactcctt cctctccgag agaaatttcc aatctgaccg
    54601 tcgcgggaac agtgatggaa atctttttag atggcaacac agcatatctg agctgcctgg
    54661 gaggcggcat ccagatcgtc gacctttccg acctggaaac gccggttttg ataggctctg
    54721 ttgaaacccc cggcattgca tacgattgtt ttgtgcaagg aaatctcctg ctggtggcgg
    54781 atagtttcgg cgggttgttg attttccagc gcaacgctac aggcaacacc ttcaacaatt
    54841 ccggcagttc cactgcatgg atccagtcgc gcgatcgatt aaacaatgga gcgaatattc
    54901 ataccgtcag ctctgaacgt actgtccgat ccttcaatac agagggcata cacgaaaatc
    54961 aaaagacctc cctacaaagt tcttccacat gcaccgtcac aaccacagcg gatgatggaa
    55021 gcggcagttt gcgtgagtgc atcagcaact tgcgtgaagg tatgaccatc acttttgata
    55081 cgcagatctt ctcgccatcc aagccagcca ccatccagcc tctttctccc ctgccggcta
    55141 ttgaacagag caccttcacc atcgatgcca gcaacgcagg ggtgatcctg gatggcagcc
    55201 aattggaatc gggaaacggt ctggagtggt attcctctaa tagcatggtg atggggttgc
    55261 agatcaccaa tttcccggag aatgggatcc gtgtaaacgg cgataacaat caaatcggtg
    55321 gaaaccgcct gagcggtgaa ggcccaaccg gtgaggggaa cctgctgagc ggaaacggga
    55381 tcaacggcat tatcctgtac gggaacaaca acgtcatcag cgggaacctg atcggcaccg
    55441 atgtcaaagg caccagcgca ttcccgaatt attatggagt cttcatttca gaatggtgca
    55501 aagataccat cgtgggagga atcagcgcag gtgaagggaa tgtgatcagc ggcaacacct
    55561 ttaccaacct ggatacctgg ggcaatcaca cccaggtgat ggggaatctg atcgggttgg
    55621 acatcaccgg cagtaaagcc atcaaaacag attcccgttc gaacatcgtc atcgaatctg
    55681 gggcatccaa taccgtcatt ggcggtaccc agcccgaaat gcgcaacatc atcagcggca
    55741 gcgatctggg catcatcatg agcgacccca acagttacca aaattcggtg atcggcaatt
    55801 acatcggcac ggatataacc ggaactaaag cgatcccgaa caataccggc ttgaccatct
    55861 ggacggtcag tttcaaccgg gtgggcggta ccgcagcggg cgagggcaac ctgatcagcg
    55921 gaaatcagat cggcattaac ttgaacgggt atggcgtcac cgataatatc atccttggca
    55981 acaccattgg attggacgcc agcggagaga aactgctcga aggcagcgtt gggatctcca
    56041 tcaacatggg acaaaagcac gatatcatcg gcggttacac ctcagccgaa ggcaaccgcg
    56101 tcgccgcaag ggatattgcc atgcgcatca gcgaaccggg catagaatat ctctacatcg
    56161 ccggaaatac catcagcagt tccggaggaa gcgggatcta tttcgagagt cgttcttcca
    56221 agaattttgt acagaacaac tccttctcac agatcgcgag taatgcagta cgggtagatt
    56281 atggcatcga caaccaactg cgcgcaaata cctttgccgt gaaagcggaa aacgccatcc
    56341 tgctgctgga aaacggcaac ctgaacctgc cggctccggt ggtcgatgct gcaaccacct
    56401 acagtgttag cggaacaaca tgttccaact gtttggtgga actctacatc atcgcagaga
    56461 acacccttca atatgcagga gagaccagtg cggatgccaa cggcgccttc caatgggcgg
    56521 gctgtgaact cattgaagga aagcaagtgg tggcactgac catcgatgcg gaggggaaca
    56581 cctctatttt ttcaagtccg atcgttataa ccatcgatga aaatcatgaa ccggcaaatt
    56641 gcagcataac ggagtaaaaa gttccgtttt ctacatcccc ctttgccgag ggggaggttt
    56701 tttatttgca acgtccgggt ttcagtcaac cccatttatg gcaggaacgc cctttcacat
    56761 ttgaacagca aagccatcag gaaaaagccc caaaaacccg cacctgtttt ttcttttttt
    56821 acggtacaat ggcgagattc aacctttatt gggaaatata catttcatgc ataagaacca
    56881 tttttgtgtc cacgggcact tttaccaacc tccccgcgaa gaccccttca ccggagaaat
    56941 ccccaatgaa tatggcgccg gtccttttgg gaactggacg gaacgcatct accaggaatg
    57001 ttacctgccc aatgcacaaa tgggcaatta ccaaaaaatc agtttcaaca tcggacccac
    57061 cctgttcgcc tggatggaaa agaaacaccc cgaaaccata cgagccatcg ccgaccagga
    57121 aaaacaagtt ttccaacgca ccggcgtcag caacgccatg gcgcagccct attttcataa
    57181 catcctgccg ctggcgagcg agcaagataa agagatcttg attcgctggg ggatcaaaga
    57241 ctatgcgaaa cgcttcgggc acgcccccca gggcatgtgg ctgccggaaa ccgccatgga
    57301 cgtagtttca ctggaagcca tagctgccca gggcatccaa ttcaccattc tggcaccctg
    57361 gcaggcaaaa gacccgctgg tggataccac ccagccttac cgggtggcat tgccgagcgg
    57421 cagggagctt ttcgtcttct tctacaacgc tttcctatca ggtgagatca gcttcaatcc
    57481 cagttccagc gaaaatgccg atacctttgt ccagaactgg ctggtcccgc agatggattc
    57541 attggaagcg gatcatgaga aattggtgct ggttgcgtcg gatggagaat tatacggtca
    57601 ccaccagcgc ttccgtgaac gattcctcag ctatctgctg gatagagccg tggaaaaaac
    57661 cgatatcgca caccaattcc cagcgctgtg gttgcgcgat cacgccgtgg aacaaacggt
    57721 tgagctgctg gagaacactt cgtggagttg ccatcacggc attgaacgct ggcgcaacgt
    57781 gtgcggcgac gccccctctg ccacctggaa gaaacctgtg cgcgagttca tgaacggctt
    57841 agccgatgcg atcgacaagg aatatgagtc tcatctacag aatctgatcc gtgacccctg
    57901 gaaattgcgc gatgcttatg tggatgtgct gcttggtgaa ataagcgtta cggacctgat
    57961 cgcagagcat gctgcacaat ctctcacccc cgggcagcag gcattggcgc agctactgct
    58021 ggaagcggaa tacgagcgct tgcgcatgtt cgcttcggac gcctggttct acttcgacct
    58081 cgaccgcatc gagcttttga acgcattgaa atatgccgcg catgcagccg gtctgacaga
    58141 acaagccacc ggtcacaatc caaccgaaac ggcagtacac atcctgaccc aggcacacag
    58201 tgaggtatcg agtttaagtg gagatatcgc ttttctaggt tatctcaaga gttttgagat
    58261 gaaactcgaa caaaactctc attgacgcaa gagttccaaa aatcgcggca attcataccc
    58321 ggatgatccg gctgccaggc gcagccggct gacgattgtt accagtcgtt ccagcttttg
    58381 ttgcgcctgc ttcgtccctg cacggcaggt ctggaggatt tccacagctc cttcaaccgt
    58441 catcaacagg aaagccgcat cggctctttc tttgttgaac caggtttttc catcatactc
    58501 atggacacac aggaagtcct gaataacagg atcggcaaac cgttctgcga taaagccctg
    58561 caatgatcgt cgattcagct tgcgcaacca atctcgtatc gcacccacaa atagaatggg
    58621 gctttgtata tcgggtgcag catctttcag gtaaccactc tgccgcaagg tttcttcgag
    58681 cacggcgaaa atatgccaat cgtgcaccag tgagaaaaat tgggcggcat cgacctgcaa
    58741 agcgttgtgc aatccctcca gatagagcca gcagattgcc accagccagg tgtagggaga
    58801 ggtatgctcg cgcaggtgat cacacaggtc tatcaactct tgagaaacga gatcgggacg
    58861 cggtttatcg agccactcaa caaaaaacag agcgtttagt ttcttcttga aatcgagagc
    58921 gggcttttca atctccaggt ccctttccag caaggtggcg gcaccccgga tcaaattctc
    58981 aagtttgtgg acggcttcat tcaacacgga ttcaggggcg ctttcgctgt acacacgcgg
    59041 tctttgttcc gccagaaaat cgaaatatct cgggttgatg atctcatgca acggtttcaa
    59101 caaagggcgc aaggggagct gccactgcaa ggtgcgcaga tcctggctcc cgcgcccact
    59161 gagcgcatca tgcacctgct gccagtttcc ttcctcatca tcctcaacga tctgaaaatc
    59221 cagaaacacg tgtgcgcgat aggcatccaa ctggatatac aagccctggt gtgcgatttc
    59281 atggcagttg cggatgtact gcaacccgct caacatgtca cggaagatca caaaagtgtt
    59341 ttttccttcc ggcagatcca agccagtatg caggtccacc tggcacaggg tgccactgcg
    59401 tttatccata aaaccaacac tggtgcgcac ccagccagcc gtatcgccaa agcggttgtg
    59461 atagaccacc agcgagcgtt cctgcccggc acggttggag taggcaaaga cgttttcatc
    59521 cactatgcca tttccatcga agaaatcgta gaggtagaac tgctctacat tcgcaaaaag
    59581 gtaccgtttt ttcaaaagcg gaaagacctg ccaggtatgg cgatccacca agctctgttc
    59641 gggctgttca tcccagtagg cgcgcttgta ttccatgcca tatttttcag tcagcccctc
    59701 gatctgccca tgcccgaaca tgggcaaacc cggcatggtg atcatgagcg tgcagatgcc
    59761 aaaatactta tcgcctttac caaattgggt cgcggcagtc tgttcgtcag gattgttcat
    59821 gaaattgaca aagcgtttca ggatttgcgg gtcaaattcc agcgtgttct tcatcacctg
    59881 gcggtacttg gcattctctt cgtctcgcaa cagattcata aaggcggaat tatagacacg
    59941 gtgcataccc agagtgcgca caaaataccc ttccatcaac cagaaag
//
LOCUS       DAOSDS010000004        50405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_407_length_50405_cov_5.303615, whole genome shotgun sequence.
ACCESSION   DAOSDS010000004 DAOSDS010000000
VERSION     DAOSDS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 50405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50405
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_407_length_50405_cov_5.303615"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..1953
                     /locus_tag="PLL88_01040"
     CDS             73..1953
                     /locus_tag="PLL88_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ00176.1"
                     /translation="MNSYPNLFKPIKINNMLLRNRIVSTPCGLVHREKACGGAALFIL
                     GGGQVDDPRGSFGRMPYVFSKYARENTRAQLDASRQGGSKVSLEIYHNGIYGIVPEGD
                     FVWGPCDGVRQDGVQIKGLNEKGMERICNLYAETAVTARDFGFDMIMLHFAHGWLPTQ
                     FLSPAWNHRTDEYGGSFGNRARFPKKIIETVRNAVGKDFPIDMRISAYEWIPESIEFG
                     DVVRFIQEVEALIDMVNVSAGLDIEHEANVHMATTFFEPHMVNVEWAAEIKKKVHIPV
                     SVVGAIMTPEEAEGIIASGKADMVSLGRMLIADPQWGKKAWEGRAEDIIPCIRCLYCY
                     HISTNRMNVGCAVNPRYGKEAIIPFELEKAMNPKKVVVIGGGPGGMQAALVAVERGHH
                     VILLEKQGQLGGLLICSDYEKYKQDLRSFRDYLFRQIEKSSVEVRLNTEATPPLVKEL
                     QPDALIIAVGGETTQPPIPGVEHARQAVGIYPELDTLKGKIVVIGGGTIGSELGLELA
                     ERGNEVHIVEITDTLNAQGNLHYRIAIRQHMAKCETLHVMTSTTCKTIKENGVIVMNE
                     GKERFIEADHILLATGLRSKKDLAHSFFDITQYTYMVGDCHRVANVKEAILSATLIAM
                     NI"
     gene            1969..3381
                     /locus_tag="PLL88_01045"
     CDS             1969..3381
                     /locus_tag="PLL88_01045"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOJ00177.1"
                     /translation="MTFPQDFLWGGAISANQCEGAWNVDGKGISTADCMTAGSVQIRR
                     EYTDGVIEGKYYPSHDAIDFYHRFKEDIALFAEMGFKCLRTSINWTRVFPNGDETVLN
                     EKGLRFYDDLFDECLKYTIEPIVTISHYETPYGLVKHYGSWRNRQMVDFYLRFCETLF
                     KRYQHKVKYWMTFNEINTIIFNPAVSTGIRIGEGENFDQVVYQAAHHIFLASAKAVKL
                     GHAIHPGFKIGMMMLGHPFYAETCKPADQWESLTKNDLMDYFSDVQVRGAYSKKALKF
                     LERKNVHLNKEPEDEEVLKQGTVDYIGFSYYHSFATSSSPEKELTAGNLLNTIKNPYL
                     KTSDWGWQYDPIGLRIEMNNLYDRYQLPVFIVENGLGAYDTVDEHGEIVDDYRIAYLK
                     EHILEMKKAINEDGVECIGYTPWGCIDLVSLGTGEMDKRYGFIYVDRDNAGRGTLERK
                     KKKSFAWYKRVIESNGADLG"
     gene            complement(3536..4717)
                     /locus_tag="PLL88_01050"
     CDS             complement(3536..4717)
                     /locus_tag="PLL88_01050"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOJ00178.1"
                     /translation="MNIQRIAVIVLDGVGAGELPDAGEYGDAGSNSLSNTARVLGGLT
                     LPNMGEIGLGNITPIVGVPPRQQTHGAYGKCREASKGKDSVTGHWELMGIEVKRPFPT
                     YPDGFPQEVLDEFKRLTGYEVLGNKPASGTEIIKELGEEHVRTGKPIVYTSADSVFQI
                     AAHEDVIPIEELYRICSIAREMLTGDHAVGRVIARPFTGENAETFTRTKRRHDYPLLA
                     PTDTMLDTLLKAGHKVYATGKIDDLFGNRGISKTHHSVFNMESIQAMVDFMDEDFSGL
                     LFANLVEFDMTYGHRNDAKGYGQKLEEFDAFIPTIRERMRESDFAMIVADHGVDPTTE
                     GTDHSREYIPLLVFGKAIKNNVNLGTRNSFADVAATIGEAFHVKAPEIGTSFLGDIWA
                     G"
     gene            complement(4820..5629)
                     /locus_tag="PLL88_01055"
     CDS             complement(4820..5629)
                     /locus_tag="PLL88_01055"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ00179.1"
                     /translation="MNLLKNDGASFWNQNPCGGEWENYRQFIDWYTATESYLLEILDP
                     SAVKGKRVLDVGCGQGVLCNLSAQMHAEVVGIDMSFSSLLEAKQGAHELGTTQAVHFI
                     QADAENLPFSHDAFDTVLSIGVLHHTPNIQDGIREIHRVIKTDGQITIMLYRRGNPKW
                     WLTKHIRKSSAVVKALSKRSRVTKGNAIAPKGTAVQELFDVPIMNAYSNKESKELFAM
                     FHNIGIRNFQPGFARLVDFLPWLKIIKPALQWIDRNAQDSWGFYQVIKANK"
     gene            5841..6695
                     /locus_tag="PLL88_01060"
     CDS             5841..6695
                     /locus_tag="PLL88_01060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ00180.1"
                     /translation="MNKPKDILMNVVCPRCQSDLKSGDHVIHCEKCGQSFSTNGNAIL
                     FAEPPAGINPSEIRERGAGKDTAWRNANGIFLKDQLRKLSKDALILDVGAGRGDFLPF
                     YSDFPHILLDVYPYPEIDVVCDLTRLNPLRAFSLDVILLMNVLEHVAAPLDLLRSMRN
                     ILKPHGKIIVAIPFYLKIHQAPLDFQRLTHFALHDLAEKAGYSVQHLEGFYDPCGVIE
                     ESLRYYRFWAIQGQGWFARKFGQAVLWHMQFLTKMLGDLSVQPYLRDPYQAEYPAPTG
                     YQLVLEKK"
     gene            6925..7302
                     /locus_tag="PLL88_01065"
     CDS             6925..7302
                     /locus_tag="PLL88_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00181.1"
                     /translation="MNQKKNISNPNIKKIYFINLLNVFLFANGILIAYYKNIGLSYIQ
                     IFAIGIVYDVFNFLLEIPSGFIADVWSRKWCVTIGHLISGLSFVLIYFAPKTFFVFLL
                     WSFLSAISSSLNSGTYTALIYES"
     gene            7303..8169
                     /locus_tag="PLL88_01070"
     CDS             7303..8169
                     /locus_tag="PLL88_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00182.1"
                     /translation="MRAVYVQKQETAYLRTQTNLSAIVLITQTIALLIGGYIADGLGF
                     EIILLISGIAGISQAVVFAFLKEPAIHALGEKGTTKLTDKLKESAQQIIEVYKSFMTK
                     ISVLFIAGFSVVFYISSGYLSTIQQPYLISIGFDSYSAISVILALINGFSALALLVYG
                     KVVPRSRSQHIIYQALIFTIGIILLGLLPDKKMIINLLILNSILEIGSITLLETLNKE
                     IPSSVRATLISTQNQINVVFNVVFGMCIGKAVDLVGINRSNFITGFFLLFATALLLFL
                     KNKKRSDEKRQF"
     gene            8468..9322
                     /locus_tag="PLL88_01075"
     CDS             8468..9322
                     /locus_tag="PLL88_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOJ00183.1"
                     /translation="MSYKNLVELFAINEGKGAVGAFNIGTLEMLPYIILAAEECNAPV
                     IVQATPKNVIDMGGYEVLGSVVKTLANSSSASVCLHLDHSKKVEFIKMAIAAGFNSVM
                     YDGSELSLKDNIENTKIVMKWCSEEGISVEAEVGKIGQSVSGIAVKNNDDELTNVEDA
                     RRFVESTDVNALAVSIGTTHWQYKSAAIINYDLLDELTSVLSTNLVLHGGTGVSNEDL
                     KKCSFHGIKKINVGTVLFRNYIKSIQSATVNVDYGSSLRTVFDQANQCIEKITIEKIN
                     AMMLRKSC"
     gene            9326..11314
                     /locus_tag="PLL88_01080"
     CDS             9326..11314
                     /locus_tag="PLL88_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HOJ00184.1"
                     /translation="MKLIETMQNRKFSKRQSEILRALLEHKDWITAKRLAVVCDSSER
                     TVKSELKDLTKLLLRSDVPLIAARGKGYRLDDIDHERAHKILEICSTDENDGDISTIR
                     ILEYLLNAKEDIDIDELSEHFFMSRSSMEMKLRNIVDLLQDKGNHVILCRRNNQVRLQ
                     GEEQYKRSIINHLVINKKTPSIVNLSRNIDGFDLGEMDDINRIVVDALQKNNVRISDV
                     GIIGITTHIIVAINRIKNGFLLEHSITSDVSAYEGVELVIANTIADEVYQKFNIQFSI
                     IEREAIAIYILLKQDSAHENKVRERQLAVGDKKYATIIDQLLDDIKDQFLIDFTTDQD
                     LFVGLVCHIKALVSRFKYLRKQTNPLLRDIKTTYPFVFEIAVYARQQLINKIGIELDE
                     SEIGYIALHFGAAIERKCEFPIEQKIKILVIGQIRSSNSLLLLTKLHSLYGDRAIIQG
                     VYSYFDASLNDCDADLVISTVPFGDDKFSCPVITVQPMLGKQDQKRITTFIWQHFNNN
                     VNYFKYMHEELFFPQLEANSAEEVIHLLCKNLFELGYADKWLEDSVIEREVISPTDLN
                     NLIALPHSLEPLGYVTSISIASLTKPILWKERNAQLIFMLTVSSDKKPYLRDFYEMIV
                     TLTEDIEYVKQLVQTKNFEEFMFILKNIQKQEWNSTRL"
     gene            11311..11802
                     /locus_tag="PLL88_01085"
     CDS             11311..11802
                     /locus_tag="PLL88_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ00185.1"
                     /translation="MNMVKTMDYFSEELILINQDYPNFESLLKAGSKVFFEKGYVKES
                     FACAVIEREKEYPTGLQTELIPFAIPHTDADHVIKPGILIMKLNNPISVKEMTNLSSS
                     IDAKLIFLIAAHNDGSQVNVLRLIISMCTDFVTVNEILLAKRPYEIYKVIEKFITNHL
                     AED"
     gene            11820..12107
                     /locus_tag="PLL88_01090"
     CDS             11820..12107
                     /locus_tag="PLL88_01090"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ00186.1"
                     /translation="MKKIIVACSGGLCTSETIASKVRRLLKERGINDVEINAIKIVDI
                     KLWQKSSDIYISVSGNEDLKYDIPTINGIPFLTGVGMQQTLEELIKLIEAP"
     gene            12165..13508
                     /locus_tag="PLL88_01095"
     CDS             12165..13508
                     /locus_tag="PLL88_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HOJ00187.1"
                     /translation="MQTLQSIVNYIINDLGSIVFMPMIMFIIGKIVRMKTKDAISAGL
                     MLGVAFSCVNAASGLLSGVMSPTLKEFVNQTGINLNTLDVSVSIRAAMTWQWPYVFLI
                     FPMQWGINFLMLLLNKTKTINVDMWNIHNKGFIALLVWHITGNVFVALGWVAVIIVIE
                     LIIGDTIQARVQELCGVPLVTVPHSELTLCAPMYAIDRYILRKIPGLDRKFDLTVLRQ
                     KIGFWSESHIIGFIVGTGLGLAARYPFAKALGIGVTLGSALVLLPMTARLFITALEPI
                     SEAAGEFMKKRFSNREVYIGLDWPILAGRNEKWVCDAILAPILILFAFITPGNRTIPM
                     ASIYVSAAMMTAAMIVTNANMIRMLILGVIFIPPSLLFATYVAPYITELATKMNAYEV
                     LEGQYITGLGQESRILRWGIIMISEGKLLGWGILAFWLATFAIFIIGMRKETEKV"
     gene            13519..14823
                     /locus_tag="PLL88_01100"
     CDS             13519..14823
                     /locus_tag="PLL88_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ00188.1"
                     /translation="MSKVSKKAATTLVETMKRIYEKGLTTMTGGNLSIKEPDGVWVTP
                     ASIDKSTLREEDMLFIKQDGTIEGNHRVTSEFPAHRAILESNVGYFNAVLHTHMPTMV
                     AYGGAHCIPNLHVLRKAYQKLGNKVGLAKYGLGGSEALAQNTAAALTYPNRASVLENH
                     AIFTAGKCMKDAYEALEIMDLVARVEGNGAILSDSAISLLNKEQRKAIDAYNAPAHDV
                     NNELLEDEESARVSDQIVLLMKRLYQRNIITGTIGTISTCLDDGRVLITPDGIDVVDL
                     QPQDIVIMKDGLRSGNGLPSDDYLLHQCIYKHNPKTKSVITASPKYIMAYGINDEPFA
                     SNVIGEAYYFISNVLKHEFGIGEEYWDFVAQKFDLLHQTAIIKNDCFIVLGKSLLNAY
                     DIIEVSESTAKGLVLTARTIKPVGLDKNSVDYYSEKMMKKYG"
     gene            14892..15719
                     /locus_tag="PLL88_01105"
     CDS             14892..15719
                     /locus_tag="PLL88_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ00189.1"
                     /translation="MTELSMETKIKLNNGVFIPQLGVGTYQINDKKQLLQILQWAFEY
                     GYRHVDTAKAYGNEAWIKDAINQSELSREEIFITSKLSVKDQREGTTKTAVDESLKLL
                     GVDYIDLYLIHWPVTGKWLEAWKVIEEMYQRGKMRAIGVCNCKVHHLEQLMNIAQVKP
                     AVDQMEFNPLMQDYDTYHFCKDNEIAFEAWSPLGNGTLIKHPSICAIGEKYGKSAPQV
                     IIRWLLEKNIIIFPKSLEEFMLKENSEVFDFSLTAEEHEWIDSLNENKRTGPDPDDF"
     gene            15679..16134
                     /locus_tag="PLL88_01110"
     CDS             15679..16134
                     /locus_tag="PLL88_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00190.1"
                     /translation="MKIKEQDLIRMIFKKQQEELFIGENLVKRKIFTINVIAYLSCAL
                     TMICKFIFSSPNVSDKSEMLMMTGFVIFILVLSMENVKNKKIIDAKVVPDRIIQQGNK
                     YSRHFSYFVFVFVNYLMQLAVLTNDVFFNKMFYLAVFTCAVKVIFNIYL"
     gene            complement(16272..17021)
                     /locus_tag="PLL88_01115"
     CDS             complement(16272..17021)
                     /locus_tag="PLL88_01115"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ00191.1"
                     /translation="MEKQHHIQLLPGDVGETVFLPGDVSRAKVIADHFDSAELVASNR
                     QYNTYTGMYKGVKVSVTSTGIGGAAAAIAIEELVKVGAKNLIRIGTGGMFQTWLPVPG
                     ILVITGAVSGDGLTREYFPQGYPAVADLDIVESMLASARELGIEVHEGYEWSHDSFYA
                     GSVFSNLDILEIEKPWIDAGVLIASQEASTVLTLATARKVRGGVILASAGCHQHPEIT
                     ATEEQMVKAISDATKISLETALKLHRLDHGE"
     gene            complement(17048..17800)
                     /locus_tag="PLL88_01120"
     CDS             complement(17048..17800)
                     /locus_tag="PLL88_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ00192.1"
                     /translation="MNQEPHRQHHIDMLPGDIAPTVLVPGDPGRVALFAEQMDEAHKV
                     AQKREYLTYTGKKDGVAVSCTSSGIGPSPTAIGTEELIRIGAKNIIRMGTCGAIQPFM
                     KAGDIIIVTAAVRGERATEEFISIDYPAIASHYIVRAGIEACKRLKLTYHVGMVRTHD
                     AFYLESPWAFGDYKARLQKWVDLGVLAVENEAATMFVVASMQGARAGAILLSSDPIFG
                     GSENDPDLDLHKQNLVRVSIETAKILHEMGLD"
     gene            18111..19292
                     /locus_tag="PLL88_01125"
     CDS             18111..19292
                     /locus_tag="PLL88_01125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00193.1"
                     /translation="MEFKKLWTRDFVLVVLANGLAFSTICVTGAVLSLYIVRQFDGDA
                     VQVGLISSLMTLSTFLFRPFTGYLVDRFGRKWTLTAALILDALISFSLLLPTGLVGLG
                     ILRFLIGFPFALFSTGLNTLTADLIPEERRSEGLGISSIVTTISGQVLAPILALWLLG
                     DANFHSVFIFAGLLAVIATCLVLLTRTADIKNGSLSFSFASLIEKRVLWLALVMGLTF
                     LGWPGLLTYGPLMAEEAGFINASPFLMAFGIGLLFSQLLGKRLFNLTAPRRAGGMALL
                     ILVSGFVLVGFVHVKLAFLLGGGLMGTGFGLSFAIFPVMAVNLVEADRRGACNATIIF
                     GQDVGVFIGSYVFGWTARAAGNYGSSYGLVGLLLLLPLAVFLFSALPDYQRRVKSSVT
                     D"
     gene            19490..20419
                     /locus_tag="PLL88_01130"
     CDS             19490..20419
                     /locus_tag="PLL88_01130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOJ00194.1"
                     /translation="MSQFTQEETKRILVNGKAVDQHDTQKILQNRSNGFDRKRKTSRG
                     GCGCVLVLMFVILLVYLLFPYSTRFLLLGIDRAPQGTLAGRSDTIMLISVNPLRPQVK
                     LFSIPRDLWVSIPGYGENRVNAAHFFAELQQSGTGPQLALQTIRSNFGVDLHYYVRLS
                     LDGLPALIDAMDGISLDLPQPMAGLPAGTNHLDGTQALAFLRSRSDGDDFLRIRQGQL
                     FISAFAKQLLDPYTWLSLPRIVAALPRALDTNLPLWLLPRLFLAFVRASIGGIIETHT
                     IDRSMVTPTLTSEGARILLPDWTIILPYLRENF"
     gene            20689..20928
                     /locus_tag="PLL88_01135"
     CDS             20689..20928
                     /locus_tag="PLL88_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HOJ00195.1"
                     /translation="MIIDLLLFILMIGFSVYAVFSRRLLNAAVALGLSSALLAIIFFR
                     LQANFAAGFELSVGAGLISLLFIMAISFARRKDTT"
     gene            20903..21181
                     /locus_tag="PLL88_01140"
     CDS             20903..21181
                     /locus_tag="PLL88_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00196.1"
                     /translation="MPEEKTQLNMSRIFYVAKLLLFVLLVCYVSSACIPMESAANASD
                     TLPDGSQENISFQEYLWQQHSLDLMIQIGLLLAGAFGVAGLLPAPDEE"
     gene            21201..21503
                     /locus_tag="PLL88_01145"
     CDS             21201..21503
                     /locus_tag="PLL88_01145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HOJ00197.1"
                     /translation="MNLSLFHYLSAALFFLGWVCLLTQRNAFKQVIGLKILLQGVTLA
                     LLLAGKDVGDVNLAQVMVISALIVETMVIALALAMIVSVFHIVPSGDIDLLRKLKG"
     gene            21500..23398
                     /locus_tag="PLL88_01150"
     CDS             21500..23398
                     /locus_tag="PLL88_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOJ00198.1"
                     /translation="MMAQLLALSVLVLPVMLAIVLLTFPTHWKGKKTFLWSFLVLSLV
                     ILMIAAAFFADDPPRSCVSWQPVSGEMCVSFGRSTLVVVFLVTASLLVWLLMVRWEGE
                     IASPQQTAFALISLSMANIAFLAEHFLLRYVALEFVGLGVVVAAWLFSTPREKTWSAV
                     KSIFINFRIGDLGLLISIFGMYSISGTFHIDQNLMLATLADPTMRAVLILGFALAVCV
                     KMAVFPLDRWVDACASLPRSLKIWFTRLLLPALGAYLLYRIAPLLLSGIVSATWILTL
                     LCALFLIKTLVFSPAVRRSDLERDLLMFFALCLLLLAAFVEQDLLWALLLLWLLIRAA
                     FAFCTGQDESCSLLRQYRKGLTVYFPYFLISGFAMIVLWWMFSLPQPIPPFLVAVYLL
                     LFGIQFLHIQRFMIVNEDESGVRVESKKVKHVLKLDFFNFLTAVFILAVFSVVIFFVT
                     RLIKGEEIWVVPVTLAFQNASFSGGHLVLTFGALAVVVMLANIFGKFATKLTAFSHEK
                     FHALHSFSKKIIPSASDRLDVSNALSAAFVRIATYVYEHVEHNLLEKFVAVFARVFRF
                     LFMTVEKFTSADLWKHALDSVMKSSRRLQRMHAGLLRINLFWLFFVLVVLIVFVLVVY
                     RGAVRLVG"
     gene            23415..24881
                     /locus_tag="PLL88_01155"
     CDS             23415..24881
                     /locus_tag="PLL88_01155"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOJ00199.1"
                     /translation="MNTQELCKVSVVLIKLVVAVFLLLLGKYIGKKAKWVALGTSVLS
                     TVLFLAYLPALQAGVNTPFDIEYLPLLGIRLSLHIDWLSFPFLLTEEFVTIFAVIFAL
                     DYVKQDHRTHLFYALLLVFSAGMSGTTLANDIFLFYFFWELMLVASTLLLLNWGDSEK
                     RTAIALKYFIITHLGSLLVLVAFVLIYAQGGIDHFSSLQAAFQMFPDKLPLIGSFFLI
                     GFGIKMALVPFHIWLPDTHSEAPMPVTIMLAAAMLSMGTYGILRFPMSFFTMQQMLVF
                     SFPMMLVGVISEIYGAIMALVETDIKRIIAYSSVSQMGYIFFGLGTLTYAGVTGATLH
                     VIYHAIVKALLFCCVGWIIKAVGERDINQIKGLGKTAPLLCACAIIGALAIAGVPALA
                     LFNSEWLIFSSGFQTPYLSLAIFEVLGSLLTVIYILRFIGQCFFVPGEPSKVKPVPLS
                     IKVATVSLSALTIIAGLFPKPIFDYVVNELPSILGGNL"
     gene            24881..26389
                     /locus_tag="PLL88_01160"
     CDS             24881..26389
                     /locus_tag="PLL88_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOJ00200.1"
                     /translation="MNLKMLSYPQIPLLFLGVTAVLIFLLARFLRVKNTVMALITAGA
                     FLLVSFYLLYEAHWIRVLELCTPNFPNALTEMFTEGAFTANDPAAVLIGVVACGLVFL
                     ISLYCVEYLKFDRRQHVFYPLLILMICGLLGMLFADDLMVVYLFCELMSICAYALVAF
                     RRKIDAAIEAGFKYLIMGSVASVVLLMGIALIFQSAGTVDLSKVEDTSGLFAQIGMIM
                     LLASFSLKSALVPLHTWLPDAHGRAPSSISAVLSGVLVQGVFYTMLRICLALGMDRFV
                     LGSYLLALSLLNILVGNLVGLVQTHTKRLLGYSTIAQMGYIGLCFALGLRNQSLLALQ
                     SGFFLIVAHAIAKSLAFLVQGVFYYYLDASQIVDLQRAGRLPYFFSLAFGISVLSLAA
                     IPPLPGFMGKWIFLTSVFAQIDAWSMVGVIALLVGSLVSLGYYFKILQNLFFRPAPPM
                     TADNSANKQKVSVWMLIPVAGLVFSIVMISITPHTLLDGTGNAAQYLMELMK"
     gene            26391..26771
                     /locus_tag="PLL88_01165"
     CDS             26391..26771
                     /locus_tag="PLL88_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00201.1"
                     /translation="MDNTTFWFSPPVLMIVFLLLGGFMLFIGKMISAKGRHSRAKYLH
                     YSCGEELNHSSLSLNYHAFFRLALLFSILHIVALIISTMDPNADTKLYPVLYLLGAGV
                     SMFILLERDEKKQHQSIQHRFLGA"
     gene            26722..27162
                     /locus_tag="PLL88_01170"
     CDS             26722..27162
                     /locus_tag="PLL88_01170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOJ00202.1"
                     /translation="MKKNSIKVSNIVSWARKKSPWILHFNSGGCNGCDIEILSLLTPL
                     YDVERFGILKESSPRHADILICTGPVTEQVKPRLQRIYDQIPEPKWVLAVGSCSCTGG
                     IFSGSYNILGGIDKLLPVDMYIPGCPCRPETIIDGIIKLVELME"
     gene            27159..27641
                     /locus_tag="PLL88_01175"
     CDS             27159..27641
                     /locus_tag="PLL88_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOJ00203.1"
                     /translation="MKMEDIVNDLMATLGLDNLQTMLGIPDDEQVIHLPAAQLLTAVE
                     KLLELGVWHLSAITCLQEKCFILLYHFWCFGNLTLRVEVAEGTPCIRSVSALIPGAEY
                     YEREIREMYGIEFEGLTNPSSLFLPDNWHAGYPMRTTDEQNAVAGENERKNGMQGGTS
                     "
     gene            27638..28720
                     /locus_tag="PLL88_01180"
     CDS             27638..28720
                     /locus_tag="PLL88_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOJ00204.1"
                     /translation="MNKVVVPIGPQHPLLKEPMSFLISLEGEQVVDSVLRLGYVHRGI
                     EKLAQQRTYIQNVYLIERICGICSHIHTTTYCQAVETLLGLEVPKRGLYIRTLLCELE
                     RIHSHLLWLGVLSKNIGLDTIFMYAWRDREIVLNLMEEISGGRVSHAVNVIGGVRVDL
                     DAMHIATLQNGLAELNDRVEELTRSIEHDRSFAARTRGIGKLTLEDVKRFCVVGPVAR
                     ASGLNIDLRRDAPYGAYRDFEMEVPIMDQGDVWARSMVRMYETVQAIRLSRRIVEALP
                     EGAVSVKAPRKVPQGEIVSRAEAPRGEVFYYVRSDGTQVPARLKVRTPTLTNLITLSK
                     QLKDVQMADVSSVIAGVDLCIACADR"
     gene            28740..29666
                     /locus_tag="PLL88_01185"
     CDS             28740..29666
                     /locus_tag="PLL88_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOJ00205.1"
                     /translation="MNVLQAMFQIFIFPGFVFLFVSALFFHWLDRKLIARFQGRIGPP
                     WYQPLADFLKLFAKEDVLPQGTNLLFASLLPLVSFASVLTASFYLPVAVLTAPSFQGD
                     LIVVLFLLSMPSLLYFLAGWITGGVYSILGGIRSLLQYFSYEIPFLLAVSGTAIASGS
                     WSLADIRAAQLSSGAYIIPQWFGFLLAVSGLIGKLKREPYDIPKAQSEMVAGPLTEFS
                     GKKLGLWYLTNHLQTVVGLAFLVNVYFAGYWEMNTLGGFIICYLLLILLQCLLSAISA
                     IYARLRIDQLISLNWRVLIPLTLLHNLFLLLK"
     gene            29675..30049
                     /locus_tag="PLL88_01190"
     CDS             29675..30049
                     /locus_tag="PLL88_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ00206.1"
                     /translation="MKLRSFFYLLPNIITNIFSKPRTMNYPTEPAAVSVNFRGSVRVH
                     SSNCVGCSLCVRDCPAFALELEGETNGRFRLIHYRDRCTYCGQCELSCRYKALYLDHA
                     YDEPSSDRRNFIIVLVDHMAED"
     gene            complement(30220..30648)
                     /locus_tag="PLL88_01195"
     CDS             complement(30220..30648)
                     /locus_tag="PLL88_01195"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00207.1"
                     /translation="MKIRFLSLLVFGALILSACASGTAVETATAPAMDTSEATDVQSA
                     DSEVVDTADENVVSFSNDIWPVIEQFALPAHGSEMKGGVQLESYDQIMRYVVPGDPEA
                     SLLYKYLTGDGVPVMPPTGKLPDETIQLFYDWIAQGAQNN"
     gene            30701..30982
                     /locus_tag="PLL88_01200"
     CDS             30701..30982
                     /locus_tag="PLL88_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00208.1"
                     /translation="MYDNCKKGWAFLFFPTDLWRDWNSYSACGVNFLEERRHSGGLSG
                     DLYGDRYLISDQHVSFCGLRMPERQTRWWSPEMRSIKEDGRSCVRVECC"
     gene            31115..31876
                     /locus_tag="PLL88_01205"
     CDS             31115..31876
                     /locus_tag="PLL88_01205"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ00209.1"
                     /translation="MPHISRVSYKPMYLQICELIRQKINSGEIAIGSRVWSESDIMEK
                     FDVSRNTARKAIETLQNEGIVSRIQGKGSFVSQPMVNYGLQNLMSFSEETREKRLIPT
                     SKVIAFTREHPDATRAENLKILEDDWVFRLERVRNADGNPIAYQIAFLPEKLCPGLLN
                     YDFAERSLYDVFETDYNHVLSWQKTVIKPIVADDYLAGILKISPRTPLLYTESVTYLA
                     GGTPIESSKNIYLSERYQFTVLSHRFDPARGAENK"
     gene            31873..33198
                     /gene="purD"
                     /locus_tag="PLL88_01210"
     CDS             31873..33198
                     /gene="purD"
                     /locus_tag="PLL88_01210"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOJ00210.1"
                     /translation="MKIGLIGSGGREHAIANVLGKSPSHDALFVYGDHHNPGIQKLAA
                     ALTIASMQDVRKIVEFFTVHQVELVVVGPEVPLIAGVSDRLRAQGIKVVGPAQAQARL
                     EGDKVFMRDLLRRRVQKGSPRWKEVKDVETARNFIREVGQVAVKPIGLTGGKGVSVMG
                     VHFHSMEEALARVRSSLEQDGVVLLEERLIGEEFSRMAFVYDGKLIGMPVAQDFKYAF
                     DGDQGGMTGGMGAYTMADGTLPFLTQEDLYEADSILQLTVHAIEEESGQGYRGFLYGQ
                     FIATAHGVQVIEYNVRLGDPEAINMMALLNTDAPAFLADLANGHLDESSTIFAPAASV
                     CKYVVPAAYPQSGNEHIRVDLNEKTIRQEGLSVVYASMEEKATGLETLGARAFAIVGI
                     GEDAGSISTKIENLLERIEPENLRHRKDVGDPKIIREKIRKMENLRRKK"
     gene            33200..34252
                     /locus_tag="PLL88_01215"
     CDS             33200..34252
                     /locus_tag="PLL88_01215"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00211.1"
                     /translation="MKTAKTEITALERLVEIPGLPGREGRVADFIRSSLSPACECCSD
                     NLGNLVAHIPGKGKRVMLVAHMDEVGLIVQRILPDGFLKVERMGGTSLRALPGSRLAL
                     WTSGGCIPAVAGILPQHLDSGEPISDFSRIYVDIGSASEEETRAMGVRPGNGLTWDSP
                     LRFLNASLICAKALDDRLGCFCLMQLEKVIKPDDLKCDLYLVFSVQEETMLTGGMPVA
                     NAIEPEVLIGVDGTLAFDTPDLQGKQSDIRLGCGPAVKWMDAIRGKMAAFVPDQRLTE
                     LVCRTAQSNGIPLQDEIAVGMSTAITPMVYASKGSPACAISIPIRYHHTPVETADLGD
                     VEHAVLLLKQLVEIDL"
     gene            34442..35506
                     /locus_tag="PLL88_01220"
     CDS             34442..35506
                     /locus_tag="PLL88_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ00212.1"
                     /translation="MKSKLFTFFALLIAVGMLLTACSNGAEEAPASEEAVAEAESLKV
                     IHLVNGVLGDKSFFDSAERGIKRASEEFGFEYKTIEMGTDPTVWESGLADAAANEDYD
                     IMIMGTYQMIGYLEAIAGNYPDKYFMIYDSAATPENCSNGCANVYSITYKQNEGSYVA
                     GVYAGLMTQTGIVGVVGAQDIPVINDFIVGYTQGVKSVNPDYQVLLNYAGGWNDPAKG
                     KEQALAMYQQGADYVFQVAGATGDGIFQAAQETGNYAIGVDSDQATIIKDTNPELAAV
                     IATSMMKNVDNSLYRALTMHLEGTLTYGTSEALGITEGGVGIAKNEIYEALTPSEVKD
                     QITQIEADVASGKITVDTAF"
     gene            35583..37127
                     /locus_tag="PLL88_01225"
     CDS             35583..37127
                     /locus_tag="PLL88_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00213.1"
                     /translation="MAPLVEMRNIVKIYSNGVIANNGIDFQIEKGEIHAIVGENGAGK
                     TTLMKILYGSEQPTSGEILINGEPVIFKNPLDAMKKGVGMVHQNFMQIPSFTAAENVV
                     LGHEPTRGISLDKKKAVEETRKLSEQYGLLVEPEAITETINVGMRQRIEILKILYLGA
                     EILIFDEPTAVLTPQETHDLFKAVRVLIEKGKTIVFITHKLREVTEISDRVTVMRNGK
                     IMGTLKTADATREGLARMMVGREVFLDIHKQPLKRGRKVMSIKDLSYVSETGRTMLRD
                     LSFNLYEGEILGIAGVEGNGQTELVEVLSGLRAPASGSALVGETVITAKTPREVRQAG
                     VAHIPEDRLSNGLSLSASIDDNLIVDRFFKPPFKKGVIIDHKVVEDNGKRLIRQFGIL
                     TPNGELPVSSLSGGNMQKVVVAREFSSNPKILIAAQPTRGIDVGATEFIRDQLVQKRT
                     DGTAVLLVSADLSEVMSLSDRIITMFEGQITGVFPEASKVTEEELGYYMLGIKRQTAE
                     EMEALL"
     gene            37124..38266
                     /locus_tag="PLL88_01230"
     CDS             37124..38266
                     /locus_tag="PLL88_01230"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00214.1"
                     /translation="MKKFFSNPTIREGLRILLAISSALVLGFIITLFVSEDPIGAYRA
                     FLTGPFTRFNRFGDWIEESITLILVGLSVAIAFKANMFSLGTQGQLVLGALVSGVIAL
                     YVPLPTILRVPLALVCAMVVGFIWGWIPGYLKANLDANEIVSTLMLNTIAIKLYDYLL
                     VFYIKPKDAGYNVSAPFPVEGQLPSFVPNLPFLNKVYTLFTTNTNITIMLYIVVLAVV
                     VAYYLVFKTPFGYKLRMIGSNMKFARYGGIDTKMTTILVFAISGVFGALAGAHMTMAI
                     QHSIITNISVSMAFEGIIVAILARNNPLYIPLTGLAYGYLRAGANIMERSSDVSREMI
                     SIIQALIILFVTAERILPTVQRVIEERKEQNATKERSLPAGGSNEH"
     gene            38256..39233
                     /locus_tag="PLL88_01235"
     CDS             38256..39233
                     /locus_tag="PLL88_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00215.1"
                     /translation="MNINLVLQGIFSALFIASVLRISTALVLPALGGLITDLAGSINI
                     ALEGTMLVSAFTGVIVSGFSRNVWLGVLAGIVSGVAFSALLAYFHLYLGTNIILAGTA
                     LNILASGGTVFLLFMLTGDKGSSSTLASGVVPTVNIPFIQDIPFLGTVLSGHNVFVYG
                     AFLLTFLINKLLYNTKFGVHLRAVGEDPLAATSVGINVRRTKFLTILLSGFLASLGGL
                     NLSMAYLSSFQRDMTAGRGFIALAAVYLGGRKPWGTVLAAILFGIADALSNQLGTLKI
                     PSQLVQMIPYVITVLALVVNGIRNRTRLIEQTKRFQERELVRANSEKPK"
     gene            39313..39990
                     /locus_tag="PLL88_01240"
     CDS             39313..39990
                     /locus_tag="PLL88_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOJ00216.1"
                     /translation="MIIQIYAFTDVDTALKAAEFGVNHIGFVAGKYGEVPAELTFREA
                     REIAAALPPQATAVALTMATEVDEILRMVEQVNPHIVHISSDLDDVDVETMRELRSRL
                     DKNIRLMKAIPVEDETSVQVAQQYAAVSDLLLLDTKRSGFPGVGATGFTHDWNISKQI
                     VETAGVPVILAGGLTPQNVAAAIATTHPWGVDSNTSTNVPGSNTDKDLQRIADFVKAV
                     RSGENEA"
     gene            39987..40979
                     /locus_tag="PLL88_01245"
     CDS             39987..40979
                     /locus_tag="PLL88_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ00217.1"
                     /translation="MSSGEVLLLGDINIDTVLPVAEFPMPGRDGLAPHISTGIGGAVV
                     NSAFVLQHLGQRTALLGCVGADVWAEKVKQDVGQTTIKMDYVKSKPQHCTGLTFIIAT
                     PDGERTMFSHRGVNTQLAPQDIDEKAFPGAGMLHISGYALLESPQKEAVWRAVELAKQ
                     NQVPVSLDTGLDPVVRNPDEVRRLLPALTLCITGLKEMEILFGDIPLSEAVDAMLASG
                     VRLVAIKLGDKGCHVFGEAEKISYPAFQVNALDTTGAGDSFTSGLIYGWLQGMGLLPA
                     AALASALGALATTVYGAGSALPAKGKVVDFLRTVQDQSIAGEIEELLGILEKNT"
     gene            41062..41475
                     /locus_tag="PLL88_01250"
     CDS             41062..41475
                     /locus_tag="PLL88_01250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ00218.1"
                     /translation="MFAEIKVNTEKSECLMNITQEVQQAVTESGVKEGVCVVFVPHTT
                     AAVTINSCLDSMTAEDITGEIHRLVPTRVDFKHIYDTPTDAAGHIKSVLVGNSLSLII
                     KDGRLLLGGSQSILFFEFDGPRKRLVQLRIMGDPD"
     gene            41488..42420
                     /locus_tag="PLL88_01255"
     CDS             41488..42420
                     /locus_tag="PLL88_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOJ00219.1"
                     /translation="MPQKIIIDTDPGVDDTIAISVALRSPELEVVGLTSVFGNATTEI
                     TTQNALRLVELEAHDSVPVARGCDVPLVVELEAIASEVHGKDGLGNTYLPAPKGKPIA
                     KTAAEFIIDTVHKYPGEVVLVPVGPLTNIAVALKADPEIAHMLKKIVLMGGAVSRAGN
                     ISPVAEANVYHDPHAADIVMQAGCPVVMVGLDVTEEVVMTPQFFEELFRAKNPAVDLI
                     RRILPVYQNYFDRFLGMKGSIHTHDPSAIAYLLDPDLFQTASVPVFVETVGRCKGQTV
                     ADWHRQWESRAEIEVCQSVNSVGVLALIKDRLTR"
     gene            42503..43477
                     /locus_tag="PLL88_01260"
     CDS             42503..43477
                     /locus_tag="PLL88_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ00220.1"
                     /translation="MFSGGRVASMQPCHERKIDLYCLGGASVDLVLEVPRLPLAGEKL
                     LAHYYGQLPGGFIANTACAAAKLGLRTAWAGSVGDDAFGHSIMRGFTKFGVRTDDVEL
                     LKDAASDFTVVLVHPDGERTILIVPSLPIPPALDEHIRCSLQNSRIGYTVLYDHDWFM
                     EVADLLHGSGGKVAVDLELNTLRDREAASQMLKAADYVFSDKNALRELTGNADENESV
                     KEILALGAELVILTKGSEGAAAYTATGRYSTGSYQVPIKDTTGAGDCFHAAVLFGILS
                     DFQYQDFLDFASAAAAILIQEMGARQGLPTAVEVQKFMENHIRGDELK"
     gene            43474..44619
                     /locus_tag="PLL88_01265"
     CDS             43474..44619
                     /locus_tag="PLL88_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ00221.1"
                     /translation="MNYMIEQIRSLPELLKQIYEPLDDTIRRKVDHELCLSAKRLYVV
                     GCGDSHHAALSTEFAFETLTKLPDDPMPSMLFGRYAAGLIPPSGPKTNIVIGISVSGA
                     VSRTAEAVRMGRQAGATTLALTATPGSPLAKEADVMVDVPVPSFPDPVNMVVPGVRSY
                     FTSQVGLYLLAIRFAEVRGTLSNAEVAAHRAKVHDFTSAISHTIEMCDPLARQAAQAW
                     KGDEEFVFAGSGPNFATSLFVAAKVLEASGDSALGQDVEEWAHLQYFAKKAPTPTFII
                     SAGDRDLSRAAEVAVAARQIGRKVAVIAPASATELVKLADYHFPIPQVDEMFSPLITA
                     IPGELFAAYLAEEKKEPYFRDFKGGRDVEGGGGISRIRTSETWEQWQ"
     gene            44619..45434
                     /locus_tag="PLL88_01270"
     CDS             44619..45434
                     /locus_tag="PLL88_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOJ00222.1"
                     /translation="MSDKQLFPSVKGAAIIAGLHLPPSPASNQTTSVPMDSIIDFALK
                     NTEKAVRAGVPALYIQDLADLPVAPTVQAHTVAFLSVVGAAIRKEFPQLVLGVCMMAH
                     GAREPLAVAQAIGAQFVRIKVYSGAMVKAEGLLQGCAYDAITYRKMVGAENIAILADV
                     YDRTGTPLGELPIAEAARFAGVNCKADGLVLTGLSFTESLSMLQEVQKAKLGIPLFLG
                     GGARAENVKEALKFCDGVIVSSSFKPVGGWTEEAILAEWDYERIARFMQAVNA"
     gene            45473..46387
                     /locus_tag="PLL88_01275"
     CDS             45473..46387
                     /locus_tag="PLL88_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ00223.1"
                     /translation="MFISIGYVIIDDIVLPDGTKHMGCLGGGSLHAIMGMRVWSDQVG
                     LVGFVGSDFPSDLRDDLSKLFDLRGLTTLQARTMRAWQYFEADGTRNETFQTDPNEFP
                     LFHPQVASFPPDYNRLQGVHLHCSWAEVAEWVEYLEGLGNPFILWEPIQAEFLPENRD
                     NFLKILPLVDCVSPNLQEAQALLGAPDARNQLDMLVQNGARMAVIRAGAAGSTYADSE
                     GNYIRVPAVKVEKVVDQTGAGNAYCGGLIVGCQQADDPREALCRAAVSASFSLEQFGA
                     IFPVKGIEKAAQRRLDECREELYRLNGN"
     gene            complement(46459..47613)
                     /locus_tag="PLL88_01280"
     CDS             complement(46459..47613)
                     /locus_tag="PLL88_01280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00224.1"
                     /translation="MKKFLVGGKTQLAVLAAFFTNGAVGATWVARIPVIQNKLALSEG
                     RLGLILFGAALGMITALSLVGSLIERFGSAKVSLVSSLILCAALPLIAITPDPISLFF
                     TLFIYGAAMSSMDIAMNEQAVRVERKNGRALMSSFHAGFSIGGLAGALLSAGVASLPA
                     VTILAHFMLVAALFITVTVVFYPYLQPGKQATEERRSMFRFPERALWAMGAIAFCSVI
                     GEAAMTDWSAVYLSRVLSTSSSLAALGYAAFSLTMTVGRLSGDHLVRKWKPVNLVRAG
                     SITAGLGLVAIILTANPVVAIAGFAMVGLGLANIIPLTFSAAGNFPGIPSGVGIASVA
                     TFGYTGSLITAPMIGAVAEATSLRVSMLLVAILVSTLLISARSIAHQPRK"
     gene            complement(47661..48431)
                     /locus_tag="PLL88_01285"
     CDS             complement(47661..48431)
                     /locus_tag="PLL88_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ00225.1"
                     /translation="MQTIDLPQERQQAILDELHLRGRVVASELSSQYGVSEDTIRRDL
                     RDLAAAGLLKRVHGGALPIAPNNIPFTERDKQNPFGKIKLAKAARELVRDGQLILLGG
                     GTTNTSLAKNLPPDLRATIVTISPQIALHLANYRNIAVILIGGHLYKRELVTSDTEAI
                     EQIRRFQADICFLGICGLHPEVGCSIYDYEEVNMMRAFIAQSGEVVATVTEEKLGTVG
                     PYVVAPCTAITHIITEKQVKEEILVPYRNLGTQVFKAE"
     gene            48650..>50405
                     /locus_tag="PLL88_01290"
     CDS             48650..>50405
                     /locus_tag="PLL88_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ00226.1"
                     /translation="MEFHISSKARKKYQFEESLFSFDGNIIFANFHAARLFAQRMNEK
                     RDLTAHPDLTVRAGQVNAVGLIDEILHYVVSIYRTQKAPRLYQDLEAALRKGLGKRKL
                     TAVLRAFTRDFPPAAVFQEKMMPDEYLSGETNGIPNHHSTLEEILILWITNQNPACLP
                     YQELFDDRDLGAKTSYSDLITTLHAFFETQATFGPDNQNLVDMLRSPAIAVPDSLRGQ
                     LDYMRSRWGDLLGHYLLKLLGSLNLFAEEEQMRGMGPGPVRIPVYAAPDGLEAERFSP
                     DADWMPRLVMMAKNTYVWLDQLSKRYHRPIIRLDQIPDEELERLADCGFTGLWLIGLW
                     ERSRASARIKQACGNPEAIASAYSLKEYQIAGDLGGEEALINLRTRCQQRGIRLASDM
                     VPNHMAIDSTWVVEHPDWFVSMPYSPFPSYTFRGRNLSEDERGEIYIEDHYYDRTDAA
                     VVFKYHDRRRDRTYYIYHGNDGTSMPWNDTAQLNYLDPNVREAVIRTILDVARRFPII
                     RFDAAMTLAKRHYQRLWFPSPGGGCDIPSRSDFSLPQDIFNQYMPNEFWREVVDRVAA
                     EAPDTLLLAEAFWLMEGYF"
BASE COUNT        12385 a        11069 c        12879 g        14072 t
ORIGIN      
        1 gacagggtcg gcaaggtgtt ggaagcgacc aatgacgcgt attttatcgc tgcgaatctg
       61 taagagtgaa gtatgaatag ttacccaaac ttatttaaac caataaagat caataacatg
      121 ctgctgcgaa atcgcatcgt gtctaccccc tgcggcttgg tacacaggga aaaggcatgt
      181 ggcggagcgg ctctgttcat tttaggcggt gggcaggtgg atgatccgcg tggctctttc
      241 ggaagaatgc cttatgtctt ttcaaaatat gcaagagaaa acacgcgagc gcaattggat
      301 gccagccgcc agggaggatc aaaagtatct ctggagatat accataacgg gatctatggg
      361 attgttccgg agggggattt tgtttgggga ccctgtgatg gcgtgagaca ggacggtgtt
      421 cagatcaagg gcttgaatga aaagggcatg gaaaggatct gcaatttata tgccgaaact
      481 gcggtcacgg cgcgcgattt tggttttgat atgatcatgt tacattttgc gcacggctgg
      541 ctgccgacgc aattcctctc ccctgcctgg aatcaccgta ccgacgagta tggtggtagc
      601 tttggaaacc gggcaagatt tccaaaaaag atcattgaga ctgtaagaaa tgccgtggga
      661 aaagatttcc caatcgatat gcgaatcagc gcttatgaat ggatcccgga aagcattgaa
      721 tttggtgatg tggtgcgctt cattcaggaa gtagaagcgt tgatcgatat ggtcaacgtt
      781 tcagccggtc tggatatcga acatgaagcc aatgtgcaca tggcaaccac cttctttgag
      841 ccgcatatgg tgaatgtgga gtgggcggca gagataaaga aaaaagtgca tattcctgtc
      901 agtgtggtgg gtgccatcat gacgccggag gaagccgaag ggatcatcgc ttcgggaaaa
      961 gcggatatgg tatcgcttgg gcgaatgctg atcgctgacc cgcaatgggg caagaaagca
     1021 tgggaaggca gggctgaaga tatcattccc tgcatacgct gtttgtactg ctatcatatt
     1081 tccaccaatc gcatgaacgt tggttgtgct gtgaatcccc gctacggaaa agaagctatc
     1141 attccatttg aattggaaaa agccatgaat ccgaaaaagg tggtggttat cggcggtggt
     1201 cctggcggca tgcaggctgc tctggttgct gtggagcgcg gacatcacgt catcttactc
     1261 gagaaacaag gacaattggg cgggttgctc atttgttctg attatgaaaa atataaacaa
     1321 gacctgcgca gttttcgaga ttatctgttc cggcagatcg agaaatcttc tgttgaagtg
     1381 cgcttgaaca ccgaagcgac cccaccactg gtcaaggagc ttcaaccaga tgctctgatc
     1441 atcgcagtag gaggagagac gacccagccc cccatacccg gggtggaaca tgcccgtcag
     1501 gcagttggca tctatccgga gttggatacc ttgaaaggga agatcgtggt gatcggtgga
     1561 gggacgatcg gatcggagtt gggattggag ctggcggagc gcgggaatga ggtgcacatt
     1621 gtggagatta cggatacgct gaatgcgcag ggtaatctgc actaccgcat agcaattcga
     1681 cagcacatgg cgaaatgtga gaccctgcat gtcatgacca gcacaacatg caagacgatc
     1741 aaagaaaatg gggtgatcgt gatgaatgaa ggcaaagaac gtttcattga ggctgaccat
     1801 attctgctgg cgacgggact gcggtcgaag aaagatctgg ctcattcgtt ctttgacatc
     1861 acgcagtaca cctatatggt gggtgattgt caccgcgttg ctaatgtaaa agaagcgatc
     1921 ttatccgcga ctttgatcgc gatgaacata tagagaaagg aaccggaaat gacttttccg
     1981 caagattttt tatggggtgg ggcgatctca gccaaccaat gtgaaggcgc ctggaatgtg
     2041 gatggcaaag gcatcagcac tgccgattgc atgacagccg gttctgtgca gattcgccgg
     2101 gaatacaccg atggggtaat cgagggcaaa tattacccta gccatgatgc gatcgatttt
     2161 taccaccgat tcaaagaaga tattgctttg ttcgcagaaa tgggtttcaa gtgcctgcgc
     2221 accagtatca actggacgcg cgttttcccc aatggcgacg aaaccgtgct caatgaaaaa
     2281 ggattgcggt tctacgatga tctgttcgat gagtgtttga agtatacgat agagcccatc
     2341 gtgaccatat cccattatga aactccgtat gggttggtaa aacactatgg ttcctggcgc
     2401 aatcgccaaa tggtggattt ttaccttcgt ttttgtgaga cgctcttcaa acgataccaa
     2461 cacaaagtta aatattggat gacatttaat gagatcaata ccattatttt taatcctgct
     2521 gtgtcgacgg gcattcggat tggtgaggga gagaatttcg accaagttgt ctatcaggca
     2581 gcacaccata tttttcttgc cagtgccaaa gcggtaaagc ttgggcatgc cattcatccg
     2641 ggtttcaaga ttggcatgat gatgcttggt catcctttct acgctgaaac gtgcaaaccc
     2701 gcagaccaat gggaaagcct gacgaaaaat gacctgatgg attacttttc tgatgtccag
     2761 gttcgaggtg cctattcgaa aaaagcgttg aagttccttg agagaaaaaa tgttcatctc
     2821 aataaagaac ccgaagatga agaggttctg aagcagggga ccgttgatta tatcggtttc
     2881 agttattatc acagttttgc caccagcagt tctccggaaa aggaactgac tgccgggaat
     2941 ctgttgaaca ccatcaagaa cccctatttg aaaaccagcg attggggttg gcagtatgac
     3001 ccgattggat tgcgcatcga gatgaacaac ctctacgatc gttaccagct ccctgtcttt
     3061 atcgtggaaa atggcttggg agcctacgat acggttgatg agcatggtga gatcgtggac
     3121 gactatcgca ttgcttacct gaaagagcat attctggaaa tgaaaaaagc catcaatgaa
     3181 gatggggtgg aatgcatcgg atataccccc tggggatgta tcgatctggt cagcctgggc
     3241 acgggggaga tggataagcg ctatggcttt atctatgtcg atcgggacaa tgccgggcgc
     3301 gggacgttgg aaagaaagaa aaagaaaagt tttgcttggt ataaaagagt tatcgaatca
     3361 aatggagcag acctgggata gtcaaatctc ttaggagatt gcatgaccat cttgatcatg
     3421 agataagagg agggaaaacc gggcaggttg gtgaaaggta tacctgttcc aaagcgaaaa
     3481 aataaaaaat ccccctgcca tctaaaggct gggggattgc tattcgcttg tggtcttatc
     3541 cagcccagat atctcctaga aaactggtgc cgatctcagg cgctttcacg tgaaatgctt
     3601 ccccgatggt ggcagctaca tcagcaaagg aattacgagt acccaggttc acattgttct
     3661 tgatggcttt tccaaagacc agcagcggga tatactcgcg cgaatggtca gtgccctctg
     3721 tggtggggtc tacaccgtgg tcagccacga tcatcgcgaa atcgctctcg cgcattcttt
     3781 ctcgaatggt cgggataaag gcatcgaact cttccaattt ttgcccgtat cctttggcgt
     3841 cattgcgatg cccataggtc atgtcaaatt ccaccaggtt ggcgaaaagc aacccgctga
     3901 aatcctcgtc catgaagtcg accatggcct gaatgctttc catgttgaag accgaatggt
     3961 gtgttttgct gatgccgcgg ttgccgaaca ggtcatcgat ctttccggtg gcgtacactt
     4021 tatgccctgc tttcaacagg gtgtcgagca tggtatccgt tggcgcgagc agcggataat
     4081 cgtggcggcg tttggtgcgc gtgaaggtct ctgcgttttc tccggtgaaa ggacgggcga
     4141 tcacacgacc gactgcgtga tcgcctgtga gcatctcacg cgctatggag caaatgcggt
     4201 acagctcttc gatgggtata acatcttcat gcgctgcgat ctgaaagacg gaatcggctg
     4261 aggtgtacac gatgggtttg ccggttcgca cgtgctcttc ccccagttct tttatgatct
     4321 cggtgcccga tgccggtttg tttcccagca cttcatagcc ggtcaggcgt ttgaattcat
     4381 ccagtacctc ttgagggaat ccatcagggt aggtggggaa aggtcttttc acttcgatac
     4441 ccatcagttc ccagtgcccg gttaccgagt ctttcccttt agaagcttcc cggcacttgc
     4501 cgtaggcgcc gtgcgtctgt tggcggggag ggacgcccac gatgggggtg atatttccca
     4561 atcctatttc gcccatattg ggcagggtca agccacctaa cacgcgggcg gtgttggaga
     4621 gcgaattgct gccggcgtca ccgtattctc cagcgtccgg cagctctccg gcgcccacgc
     4681 catccagcac aattacagcg attcgttgaa tgttcatagt tcagttcctg tatgtttttt
     4741 gaaagtaagt tctttattat atcgccgcat gcaaatcgga aatgttatga aatcgatgga
     4801 aaagggattg gggagcagat catttgttgg ctttgatgac ctgatagaag ccccaggaat
     4861 cctgtgcatt gcgatcgatc cattgcaatg ccggtttgat gatcttcaac cagggcagaa
     4921 aatccaccaa ccgcgcaaac cctggttgaa aattccttat gccgatatta tgaaacatag
     4981 cgaacaattc ttttgactct ttattactgt aagcgttcat gatgggcaca tcgaataatt
     5041 cctgaaccgc cgtacctttc ggtgcaattg cattcccttt ggttaccctc gatctttttg
     5101 acagcgcctt taccactgct gaacttttac gaatgtgctt ggtcagccac catttgggat
     5161 tgcctcgtct gtagagcatg atggtgatct gcccgtctgt ttttatgacc ctatggattt
     5221 cccggatgcc gtcttggatg ttgggagtgt ggtgtaaaac gccgatggaa agaaccgtat
     5281 caaaggcatc atggctgaac ggcaggtttt ctgcatcggc ttggataaaa tgaaccgctt
     5341 gtgtggttcc taattcatgg gcaccttgtt ttgcttccag caaagaggag aacgacatgt
     5401 cgatgcccac cacctctgca tgcatctggg ccgagaggtt gcacagtacc ccctgcccgc
     5461 agcccacatc cagtacgcgc ttgcccttta ctgccgatgg gtccaggatc tccagcaaat
     5521 aggattctgt tgcagtatac caatcgatga attgacgata attttcccat tcaccgccgc
     5581 atgggttttg attccaaaaa gaagcgccgt cgtttttaag aagattcatt ctttgaatta
     5641 tattccttgt atgcaggttt ttcacccaac agtttactta ccccttgatt taaataataa
     5701 aaattttaac caacctggtt gataactatg catctgctag aacgggtcat tgtccgttgt
     5761 ctatcttttt ttcaataaat tctgatataa aataatataa agtcctgatt tggttaggga
     5821 tctgcgaggg gtttttatcg atgaataaac caaaagacat attgatgaat gtagtctgcc
     5881 cccgatgtca gagcgatctc aaaagtggcg accacgtgat tcactgcgaa aaatgcgggc
     5941 aaagcttttc aaccaacgga aatgctattc tcttcgcgga accgcctgct ggtatcaatc
     6001 cctcagagat tcgcgaacga ggagcaggga aagatactgc ctggcgcaat gctaatggga
     6061 tcttcctgaa ggatcaactt cgaaaattgt ccaaagatgc tttgattctc gatgtgggtg
     6121 ccggacgtgg agattttctc ccgttttatt ccgattttcc acatatcctg ctagatgtgt
     6181 atccttatcc agagatagat gtggtatgcg acctgacccg cttgaacccg cttcgcgcat
     6241 tcagtttgga tgtcatttta ttgatgaacg ttttggagca tgtcgcagca ccgctcgacc
     6301 tgctgcgctc catgcggaat atcttgaaac cacacggaaa gatcatcgtt gccattcctt
     6361 tttatctcaa gattcaccag gcgccgcttg atttccagcg cctgacccac tttgccctgc
     6421 atgatctggc tgaaaaagcc ggttactcgg tgcagcattt ggaagggttt tatgacccgt
     6481 gtggagtgat cgaggaatcg ttacggtatt accgtttctg ggcgatacag gggcagggct
     6541 ggtttgcgcg caagttcgga caagctgtgc tgtggcacat gcagtttttg acaaaaatgc
     6601 tgggcgatct ctccgtacag ccttatctca gagatcccta tcaggcggag taccctgctc
     6661 ctacgggtta tcagttggtg ctggagaaaa agtgacgaca gatctctgtc gatggctgtc
     6721 tgatatggaa tgcactgaag aaaaaacaac cattaattaa ttgaagtgat ggatcgctta
     6781 tatcaagaag aaaagtaaat tttctttaaa agatttttat aaattttata tttggtttat
     6841 atctaatcag gttcattgct ttcagtaata aaagtgcaag cgagaataaa aatattcact
     6901 atatgaatac gttgtggaag atagatgaat caaaaaaaga atatttccaa tccaaacatc
     6961 aagaagatct attttattaa tctactaaac gtatttttat ttgcaaacgg catattaatt
     7021 gcttattaca agaacatcgg attaagctat atccaaatat tcgccatcgg aattgtatac
     7081 gacgtattta attttttgtt ggaaatcccc tcaggattta tcgcagatgt atggtcaaga
     7141 aaatggtgcg tgaccatcgg tcatctcatc tctggactta gttttgtgtt gatatatttt
     7201 gcgccgaaaa ctttttttgt tttccttctt tggtcatttt tgtctgccat cagctcttcg
     7261 ctcaattctg gaacatatac tgctctcatt tacgaatcgt aaatgagagc agtatatgtt
     7321 caaaaacaag aaaccgccta tctgcgaacg cagaccaatt taagtgccat tgttctcatt
     7381 actcaaacca ttgcattatt aataggcgga tatattgcag atggtttggg tttcgaaata
     7441 atcttgctga tttcaggaat cgcagggatt tctcaagcgg ttgtttttgc ttttctcaaa
     7501 gaacccgcta tccatgctct aggcgaaaag ggaaccacga agcttaccga taaacttaaa
     7561 gaaagcgcac aacagataat cgaagtctac aaaagcttca tgaccaaaat cagtgtcctg
     7621 ttcattgcag gattttccgt agtattttat atttcgtccg ggtatcttag caccattcag
     7681 caaccctatt taatatccat cggttttgat tcgtattcag cgatatctgt cattcttgcg
     7741 ctaatcaatg gcttttcagc gcttgctttg cttgtgtatg gaaaagtggt gcctagaagc
     7801 agatcacagc acataattta tcaagcgtta atttttacga tcggaattat attgttgggg
     7861 ctattgcctg acaaaaaaat gattattaat cttctcatat taaattcaat attggaaata
     7921 gggagcatca ctttgctgga aacacttaat aaagagattc catcgtccgt tcgagcaacg
     7981 cttatctcca cccagaacca aattaacgtg gtatttaatg ttgtatttgg tatgtgtata
     8041 ggaaaagctg ttgatcttgt tggaataaac cgatcaaatt tcataacagg atttttcctc
     8101 ttgtttgcta cggcgttatt attgtttctg aagaacaaaa agcgatcgga tgagaaaaga
     8161 caattctgaa aagaaatcgc ttgaaaaata tcttgcagga aaagcaggag atcgtacgtc
     8221 agcttttctg tacaatgagg tcggttcctt tcagttattc caaaaaaaga ttgaataact
     8281 tctgatagcg tattttgtaa aatcgagtgg tgtacttggc tcagatttga tcgaagtttc
     8341 ttttgtgatt ttttaccgtc aggcttgcga atcccaagac tttattgcat tacgcaaatg
     8401 caatagttta tttgtttttg aaattttgct gcaatagaat ggaaagtaat gtagtcggtt
     8461 gaactctatg tcttataaaa acttggttga attatttgca ataaatgaag gcaagggagc
     8521 cgtaggggct ttcaatattg gtacattgga aatgttgccc tacattattc tggcggcaga
     8581 agaatgcaac gctccggtga ttgttcaagc tacgccaaag aacgtcatag atatgggagg
     8641 atacgaagtc cttgggtctg tagtaaaaac tttggctaat tcgtcgtcgg cgagtgtttg
     8701 tttgcatcta gatcacagca agaaagtgga attcatcaaa atggctatcg cagcaggttt
     8761 taattccgtc atgtacgatg gctctgaact ttcccttaag gacaacattg aaaatacgaa
     8821 aattgtaatg aaatggtgct ccgaagaagg tatcagtgtt gaggctgaag tgggtaagat
     8881 tggtcagagt gtatcaggaa ttgccgtaaa aaataatgac gatgaattga ccaatgtcga
     8941 agacgcgagg agatttgttg agagcactga tgtgaatgcg ttagcggtat cgatcggtac
     9001 gacacattgg caatataaat cggctgccat aatcaattat gatttactag atgagcttac
     9061 cagtgtattg tcaaccaatt tagtactgca tggtggaacg ggggtatcga atgaagattt
     9121 gaaaaagtgt tcattccatg ggattaaaaa gatcaatgtt ggaacagtat tattcagaaa
     9181 ttatattaag tcaattcaat ctgcaacagt caatgtagat tatggatcat cattgagaac
     9241 ggtttttgat caagccaatc aatgtattga aaaaataacc attgaaaaaa taaacgcaat
     9301 gatgttgcgt aaatcgtgtt gacctatgaa attgattgaa accatgcaaa atagaaaatt
     9361 ttctaaaaga cagtctgaaa tacttcgagc gcttctggaa cacaaagatt ggataacagc
     9421 caaaagactg gcagttgtat gtgattccag cgaaaggact gtgaaaagtg aattgaagga
     9481 ccttaccaaa ttgttgttga gatcagatgt accccttatt gctgcaagag gtaaaggcta
     9541 tagattagat gatattgatc acgaaagagc gcacaagata cttgaaattt gcagcacaga
     9601 tgaaaatgat ggagatattt ctacaataag aattctggaa tatctgttga acgccaaaga
     9661 ggatattgat attgatgaat taagtgagca ttttttcatg agtcgatctt ccatggaaat
     9721 gaaactgaga aatattgtgg atctattaca ggataaagga aatcatgtaa ttttgtgccg
     9781 tcgtaataat caagtgcgtt tacaggggga agaacaatac aagagatcaa tcattaacca
     9841 tttggtaatt aataaaaaga cgccaagcat agtaaattta tcgagaaaca tcgatggttt
     9901 tgatttaggg gagatggatg acatcaacag gattgtcgtt gatgcccttc aaaaaaataa
     9961 tgtcagaatt tctgatgttg gcatcattgg tatcaccact catattattg tcgcgataaa
    10021 tagaataaaa aatggtttct tattagagca ctccataacg tcagatgtgt ctgcttatga
    10081 gggtgttgaa ttggtaatag cgaacactat cgcagatgag gtttaccaaa aattcaacat
    10141 tcagttcagc atcattgaaa gagaagctat tgctatctat atcttactga agcaggattc
    10201 tgcacatgag aataaggttc gagaaagaca attagctgtt ggagacaaaa agtatgcaac
    10261 gataattgat caactactag atgatatcaa agatcaattc ttgatagact ttactaccga
    10321 tcaagattta tttgtgggat tggtatgtca tatcaaagca cttgtatcaa ggtttaaata
    10381 tttacgaaaa cagacaaatc cgttgctgag ggatattaaa acaacgtatc catttgtttt
    10441 tgaaatcgcg gtttatgcaa ggcagcaatt aataaataaa atcggcatag aacttgatga
    10501 aagtgagatt ggatatatag cacttcattt tggcgcagcc atagaaagaa agtgcgaatt
    10561 tcccattgaa caaaagataa aaatattagt tattggacaa attagaagta gcaattcttt
    10621 attgctattg acaaaattac attctttata tggggatagg gcaattatac aaggtgtcta
    10681 ttcatatttt gatgcaagct tgaatgattg cgatgcagat ttagttatct caacagttcc
    10741 atttggtgat gataagtttt cttgtccggt gataactgtg caacccatgc taggcaaaca
    10801 agaccagaaa agaataacca cgtttatttg gcaacatttt aacaacaatg tcaattattt
    10861 taaatacatg catgaagaat tatttttccc tcaactcgag gcaaatagtg ctgaagaagt
    10921 aattcatctg ctctgtaaaa atctgtttga acttggttat gccgacaaat ggctggaaga
    10981 ttcagtcatt gaaagagagg tcatttctcc aaccgattta aacaatttga ttgctttgcc
    11041 acactccttg gagcccttgg gatatgtgac gtcaatatct attgcaagtc tcacaaaacc
    11101 tattttatgg aaagaaagaa acgcacaact gatcttcatg ctcacagtat cttcggataa
    11161 aaaaccatat ctaagagatt tttacgaaat gattgtcacc ttgaccgagg atattgaata
    11221 tgtaaagcaa ttggtccaaa caaaaaactt tgaagagttc atgtttattt tgaaaaatat
    11281 tcagaaacaa gaatggaatt ctacacgatt atgaacatgg ttaaaacaat ggactatttt
    11341 tctgaagagt tgattcttat caaccaagat tatccaaatt tcgaatcatt attgaaggcc
    11401 ggatcgaaag tcttttttga aaaaggttat gtcaaagaga gttttgcttg cgcggttatc
    11461 gaaagagaaa aagaataccc caccggttta caaacagaac ttatcccatt tgcgatacca
    11521 catacagatg ctgatcacgt cattaaacct ggtattttga tcatgaaatt gaataatccc
    11581 atttcagtca aggagatgac caatctgagc tcctcaattg atgccaaatt gatattttta
    11641 atcgctgctc acaacgatgg gtcacaagtt aatgtgttga gattaataat atccatgtgt
    11701 acagatttcg ttaccgttaa cgaaatttta ttggcgaaga ggccgtatga aatttataaa
    11761 gtgattgaga agtttattac aaatcatctg gcagaagatt aaagaaagga ggtaaatgag
    11821 tgaaaaaaat catcgtagct tgtagcggag gcttatgtac cagcgaaaca attgcgagca
    11881 aggttcgaag actactaaag gaaagaggaa ttaatgatgt cgaaatcaat gcaatcaaga
    11941 tagtagatat caaactatgg caaaagtcct cagacatata tatatcggtt tcgggtaatg
    12001 aagatctcaa atatgatatt ccaactatca atggtattcc atttttgaca ggcgtaggaa
    12061 tgcaacaaac gttggaagag ttgattaaat tgattgaggc accttagaaa gattctgtat
    12121 tacctgaaca agccaagtat ttttaataat aaggagaata gcaaatgcaa actctacaat
    12181 caatagtgaa ctatatcatt aatgacctgg gatcaatcgt atttatgcca atgatcatgt
    12241 tcataattgg caagattgtc cgtatgaaga cgaaagacgc gatttctgcg gggttaatgt
    12301 tgggcgttgc cttttcatgt gttaatgctg cctctggatt attgtcagga gtaatgtctc
    12361 ctacattaaa ggaatttgtc aatcagactg gcataaattt aaacacattg gatgtgtccg
    12421 tctctatacg tgccgcaatg acatggcagt ggccatatgt ttttctaatc ttcccaatgc
    12481 aatggggaat caacttctta atgttgttgc ttaataaaac caagaccata aatgttgata
    12541 tgtggaatat ccataacaag ggcttcattg cattactcgt atggcacatc accggcaacg
    12601 tttttgttgc cttaggatgg gtggcggtta tcatcgtcat tgaattaatc atcggcgata
    12661 cgattcaagc aagggtgcag gagctatgtg gggtgccact ggtaacagta cctcattctg
    12721 aattaacact ttgtgcgccc atgtatgcaa ttgaccgtta tatcttgcgg aagatccctg
    12781 ggttagaccg taaatttgat ttgacggtac tacgccagaa gattggtttc tggagcgaat
    12841 cccatatcat tggtttcatc gttggaacag ggttggggct ggctgcaaga tatccatttg
    12901 ctaaagcact gggaattgga gttactttag gttctgcgct cgttctatta ccaatgacag
    12961 caaggctatt cattactgct ttggaaccga tttctgaggc ggctggcgag tttatgaaaa
    13021 agagattctc aaacagagaa gtttatattg gactcgattg gccgatctta gctggacgta
    13081 atgaaaaatg ggtttgtgat gcaatattag ctccaatatt gattctattt gcctttatca
    13141 cgcccggcaa tcgtacgatt cctatggcga gcatctatgt cagcgctgct atgatgacag
    13201 cagctatgat cgtaacaaat gcaaacatga tacgcatgtt gatacttggc gtaattttca
    13261 taccaccttc attgttgttt gccacatacg ttgctcccta tatcactgaa ttagcaacga
    13321 agatgaacgc atatgaggtt ctggagggtc aatacataac tggactagga caggaatcga
    13381 gaatcttgcg ctggggaatc attatgattt ctgaaggaaa gctattgggg tggggaattc
    13441 tggcattctg gcttgctaca tttgcaatat tcatcatcgg gatgcgcaag gaaacagaaa
    13501 aggtttagga gttaaaaaat gagcaaagtg agtaaaaaag cagcaactac attagtggaa
    13561 acaatgaaaa gaatttacga aaaaggttta acaaccatga ccggtggaaa cctgtcgata
    13621 aaggagccag atggtgtttg ggttacacct gcaagcatag ataaaagcac tttaagggaa
    13681 gaagatatgc tgttcataaa gcaggatggg acgatagaag gcaaccatcg tgtgacatct
    13741 gagtttcccg cccatagagc aattcttgag tcaaatgtag gttatttcaa tgctgttttg
    13801 catactcata tgcccaccat ggttgcgtac ggtggggctc attgtatacc aaatctgcat
    13861 gttctaagaa aagcatatca aaaacttgga aataaagttg gactggcaaa atatggcttg
    13921 ggtggaagtg aagctttggc acagaatact gctgctgcat taacgtatcc caatagggct
    13981 tcagtgttag aaaatcatgc catatttact gctggtaagt gtatgaagga tgcttatgag
    14041 gcattagaaa taatggattt ggtggcaagg gtagaaggaa atggcgctat tttatcagat
    14101 agtgccattt ccttactgaa taaggaacaa cgcaaagcga ttgatgcgta taacgctcct
    14161 gcacatgatg ttaacaatga attattggaa gacgaggaaa gtgcaagagt cagtgatcaa
    14221 attgtattgt tgatgaaaag gctatatcag cgcaatatta tcactgggac tattggtacg
    14281 atttcaactt gtttggacga tgggcgggta cttattacgc ctgatgggat agacgttgta
    14341 gatttgcaac cgcaagatat tgttattatg aaagacggtt tacgcagcgg aaacggtttg
    14401 ccaagtgatg attacctgtt gcatcaatgt atatataaac ataaccccaa aacaaaatcc
    14461 gtaatcactg cttcgcctaa atatattatg gcctatggaa taaatgatga accttttgca
    14521 tcaaatgtaa ttggcgaggc atattatttt atatcgaatg tattgaaaca tgaatttggc
    14581 attggtgaag aatattggga ttttgttgct cagaaattcg atctgttaca tcaaacggcg
    14641 atcatcaaaa atgattgctt cattgtcctg gggaaatcac tgttaaatgc ttatgatatt
    14701 atcgaagtct ctgaatcaac tgcgaaaggt cttgtattaa ctgccagaac gataaaaccg
    14761 gtaggtttag ataaaaattc tgttgattac tacagtgaaa aaatgatgaa aaagtatgga
    14821 taagaagagg gaatagaggt cttagttaat actttctcga aaacattgac ttatataagg
    14881 agaaaacgat aatgaccgaa ttatcaatgg aaacgaaaat aaaattgaat aatggggtct
    14941 ttattccaca attgggtgtg ggcacttacc aaataaatga taagaaacaa cttttgcaga
    15001 ttctgcaatg ggcgtttgaa tatggatata gacatgttga tactgcgaaa gcatatggta
    15061 atgaagcctg gatcaaggac gctatcaacc aaagtgaatt atccagggaa gaaattttta
    15121 ttacttcgaa actttctgta aaagatcaaa gagaaggaac aacaaagacg gctgtagatg
    15181 aaagtttgaa acttcttggg gtagattata ttgatttgta tttaatccat tggcctgtga
    15241 ctgggaagtg gctggaagcg tggaaagtga ttgaggagat gtatcagaga gggaaaatga
    15301 gggcaattgg cgtgtgcaat tgtaaagtgc atcatttgga acaattaatg aatatagccc
    15361 aagtaaaacc ggctgttgat caaatggaat ttaacccctt aatgcaagac tatgatacat
    15421 atcatttctg caaagacaac gaaattgcat ttgaagcctg gagtccgctt gggaatggaa
    15481 cgttgattaa acatcctagc atttgtgcaa ttggtgaaaa atacggcaaa agcgctcctc
    15541 aagtgattat tagatggctt cttgagaaaa acataattat ttttcctaaa tcgctggaag
    15601 aattcatgtt aaaggaaaat tcagaagtat tcgacttttc attgacagcc gaggaacatg
    15661 aatggattga ttcactaaat gaaaataaaa gaacaggacc tgatccggat gatttttaaa
    15721 aaacaacagg aagaattatt catcggtgaa aatttggtta agagaaaaat attcactatc
    15781 aatgttattg cttatctctc ttgcgcatta accatgattt gtaaatttat attctcgagt
    15841 ccaaacgtta gcgataaatc agaaatgttg atgatgacag gttttgtcat atttattctt
    15901 gtcttgtcaa tggaaaatgt aaaaaacaag aaaatcattg atgcgaaagt tgtgcctgat
    15961 aggataattc agcagggtaa taaatatagt agacatttct cctattttgt atttgttttt
    16021 gtcaattatt tgatgcaatt ggcggttttg acaaatgatg tattttttaa taagatgttt
    16081 tatttggctg tttttacgtg cgcagtcaaa gttatattta acatttatct gtgagaatag
    16141 acgcgagagt cgtgtaagct aaaagtggat tctggaaaaa tcgtttgtgc ttattttctc
    16201 tttgatgcaa ggacacaaga gatcttgggg agtgtccctc cccccaaagc agggggaggt
    16261 tagaaaggta tttattctcc gtgatccagc ctgtggagct tgagcgctgt ttccagcgag
    16321 atcttggtgg cgtctgagat ggctttcacc atctgctctt ccgttgctgt gatttcgggg
    16381 tgctggtgac agccagcgga tgccagaatg accccaccgc gcaccttgcg ggcggtggct
    16441 aaggtcagca cggtggatgc ctcttgcgat gcgatcaaca cacccgcgtc gatccacggt
    16501 ttttcgatct ccagaatatc caggttcgag aaaaccgagc ctgcgtagaa tgaatcatgc
    16561 gaccactcgt aaccctcatg cacttcaatg cctagctccc tggcagaagc cagcatggac
    16621 tccacgatgt ccaggtctgc caccgccggg tagccttgcg ggaagtactc gcgcgtcaaa
    16681 ccatctccgc tcaccgcacc ggtgatcacc agaatgccgg gaaccggcag ccaggtttgg
    16741 aacataccgc ccgtgccgat gcggatgaga ttcttggcgc ccaccttcac cagctcttcg
    16801 atggcaatgg cagcggctgc cccaccaatg ccggtggagg tcacgcttac ctttacgccc
    16861 ttgtacatgc cggtgtaggt attatattgg cgattggatg ccaccagttc ggcgctgtca
    16921 aaatggtcgg caatgacctt ggcacggctc acgtcgccgg gcagaaacac ggtttcgcca
    16981 acatcccccg gtaaaagttg aatatggtgt tgtttttcca tggttatctc tccaaatttc
    17041 ctttcaccta atccagaccc atctcatgca ggatcttggc tgtctcgatg ctcacgcgca
    17101 ccaagttctg tttgtgcaga tccagatcgg ggtcgttctc gcttccgccg aagatcgggt
    17161 cgctgctcaa aaggatggcg cccgcgcgcg caccttgcat gctggcgacc acgaacatgg
    17221 tagcggcttc gttttccacc gccaatacac ccagatcgac ccatttttgc aggcgcgctt
    17281 tgtaatcgcc aaacgcccag ggcgattcga ggtagaaggc atcgtgggtg cgcaccatgc
    17341 cgacatggta ggttaatttc aatcgtttgc aggcttcaat accggcgcgc acgatgtaat
    17401 ggctggcaat ggcgggatag tcaatggaga tgaactcttc agtagcgcgt tcgccgcgca
    17461 cggcggcggt gacgatgata atatcgcccg ctttcatgaa cggttggatt gccccacagg
    17521 ttcccatgcg aatgatattt ttggctccga tgcggatcaa ctcttccgtc ccgattgcgg
    17581 tgggggaggg tccaatgcca gagctggtgc aactgacggc aacgccatcc ttcttaccgg
    17641 tgtaggtaag gtattcgcgt ttctgcgcta ctttgtgcgc ttcgtccatc tgttcggcga
    17701 acagcgcaac ccgccctggg tcgcccggga ccagaacagt tggcgctatg tcgccgggca
    17761 gcatatcgat atgatgctgt cggtgtggtt cttgattcat ggtttactcc ttgtagtgat
    17821 aattaactta attctactgg tattaaccac agtatgtcaa caacactact gtcacttttc
    17881 gttgttagac acgaactttg catgggcaag tttgatcgac aggttgtaga gcgttcattc
    17941 actcccgtaa gtttttctca ctgctccata ttttttcatt tcgatcacct ttcttacggg
    18001 tagattgaaa tccaatcgat gaattgccag gaactttcgt ggcattgatg atacacttca
    18061 tcatcttcac gagattcatt tcatcgatcc acttaaaagg tacccacatc atggaattca
    18121 aaaaactctg gacccgagat tttgttctgg tggtgcttgc caatgggctg gcgttctcca
    18181 ccatctgtgt tactggcgcg gttctatcat tgtatattgt caggcagttc gatggcgatg
    18241 cggttcaggt tggcttgatc tccagcttga tgaccctgtc gacctttctc tttcgcccct
    18301 ttaccggcta tctggttgac cggtttggca gaaagtggac gttgaccgca gcgctgattt
    18361 tggatgccct catcagcttc agcctgttgc ttcccaccgg tttggtcggt ttgggcatct
    18421 tacgtttttt gatcggtttt ccctttgccc tcttttccac cgggttgaac acgctcacgg
    18481 ctgatctgat tccggaggaa aggcgttctg aagggttggg catctcctct atcgtgacca
    18541 cgatcagcgg gcaggtcttg gctcccattc tagcattgtg gctgttgggt gatgccaatt
    18601 ttcattcggt tttcattttt gctggtttac tggctgttat cgcgacctgt ctggtgttgc
    18661 tgacccgcac ggcagatatc aagaacggtt ccctgtcttt ctcatttgct tctcttatcg
    18721 aaaaacgcgt cctctggctt gcgctggtga tgggtttgac ctttctcggt tggccgggtt
    18781 tgttgaccta tggtcctctc atggcggaag aagccgggtt catcaatgcc tcgcctttct
    18841 taatggcttt cggaattggg ctgctgttct cccaattgct tggcaaacgc ctttttaact
    18901 tgactgctcc tcgcagagct ggcgggatgg ctctgctcat cttggtgagc ggttttgtgc
    18961 tggttggttt tgtgcatgtc aagttggcat tcctgttggg cggtgggttg atgggaacag
    19021 ggtttggctt gtctttcgct atatttccgg tcatggcggt taacctggta gaggctgacc
    19081 gtcgcggagc gtgtaatgca accattatct ttgggcagga tgtgggagtt tttatcggtt
    19141 cgtatgtgtt tggctggacc gcgcgagctg ctggcaatta tggctcatct tatgggctgg
    19201 tagggctctt gctgcttctg cctctggctg tttttctgtt ttccgccctg cctgattatc
    19261 aaagaagggt taaaagcagc gtgacggatt gaaaaaattc gcttctgctg atgcccgtga
    19321 ttcaattgtc tattagcaat ctctaaagag cctgactggt tagctctgtt gtttgagtct
    19381 ttttaggctg acactcaggt tcccttgtgt gcgaggttct tctcttgcct ctgcgtcagt
    19441 tcgtgttgct ttcggcacgc atctcaccca attgggttac aatttcttca tgagccaatt
    19501 tacgcaagaa gagaccaaaa gaatattggt taatggcaag gcagtagacc agcacgacac
    19561 acaaaaaatt ctgcaaaacc gcagtaatgg ttttgataga aagagaaaaa cttcgcgtgg
    19621 cgggtgtgga tgcgttttgg ttttaatgtt tgtcatcctg cttgtctacc tgctctttcc
    19681 ttattccact cgttttcttc tgcttggaat agaccgagct ccgcagggca cattagcagg
    19741 gcgaagtgat acgatcatgc tcatatcggt taatcctctc cgaccacaag tgaaactgtt
    19801 ttccattcca cgcgacctgt gggtttccat ccctggttat ggagaaaacc gtgtcaacgc
    19861 agcacacttt tttgctgaat tgcagcaatc tggcacgggt cctcaacttg ccctgcaaac
    19921 catccgttcc aatttcgggg tggatctgca ttattatgtg cgtttaagcc tggatggtct
    19981 ccctgcgttg atcgacgcca tggatggcat ctcgcttgat ttaccgcagc caatggcagg
    20041 cttacccgct ggaacaaatc acctggatgg cacacaggct ctggctttcc tgcgcagtcg
    20101 ttccgatggt gatgatttcc tgcgcattcg acagggacag ctcttcattt ccgcctttgc
    20161 aaaacaattg cttgatccgt atacgtggtt gtcccttccg cggatagttg cagccttacc
    20221 ccgtgcgctg gataccaatt tgcccctctg gttgcttccg cgcctatttc tggctttcgt
    20281 ccgtgcaagt attggcggca ttattgaaac ccacaccatc gaccgtagca tggtaacccc
    20341 tacattgacc tctgaaggag cccgtattct tcttcctgat tggactatca ttcttcctta
    20401 tctgcgagaa aatttctgat tgagcgcgca ggtttgcata ctgtcagcca cccattcaac
    20461 cggcatagaa tgttgaatag ttccagggaa attgtttgaa ttttcgagct tttttgcgaa
    20521 taacacacaa aattttgata taatcttttt cagcgcctac ctcgacgctt tcttaaatta
    20581 atttatcctt tttgcgttga gaataaaaac aataataaat aatgcgccta cgaattgttt
    20641 cgcagacctt tatttttaat atatgccatg ccaataaagg agaaaaatat gattattgac
    20701 ttgttgctat tcattttaat gattgggttt tctgtttacg ccgtcttttc tcggcgttta
    20761 ttaaatgcgg ctgtcgcttt agggctgagc agcgctcttc tggcaatcat tttcttcaga
    20821 cttcaagcga attttgccgc tggctttgag ctgagcgtgg gggcgggatt gatcagtttg
    20881 ctttttatca tggcgatcag ctttgccaga agaaaagaca caacttaaca tgtcgcgtat
    20941 tttctatgtt gctaaattgc tcttgtttgt tctgctggtg tgttacgttt cttcagcctg
    21001 tatccccatg gaatcggcgg cgaatgcttc tgatacgctt ccggatggat cccaggaaaa
    21061 tatttctttt caagaatact tgtggcagca acactcgctg gacctgatga tccagatagg
    21121 tctgttgtta gctggcgcgt ttggggttgc ggggcttctt cccgccccgg acgaagagta
    21181 aggaaaggag gacaacaagt atgaatttga gtttattcca ttatctcagc gctgctcttt
    21241 tctttttagg ctgggtatgt ttgctcaccc agcgcaatgc gtttaaacag gtgatcggtt
    21301 tgaagatttt acttcaaggg gtaaccctgg cgctcctatt ggcaggaaaa gatgttgggg
    21361 atgtgaacct tgctcaagtg atggtgatct cagcgttgat tgtggagacg atggtgatcg
    21421 ctctggcgtt ggcgatgatt gtcagcgtct ttcatattgt gcccagcggc gatattgatc
    21481 ttctacgaaa gctgaaagga tgatggcaca attgcttgca ttgtcagtgc tggttctccc
    21541 tgtgatgctg gcgatagtat tgttgacttt cccgactcat tggaaaggga agaaaacgtt
    21601 cctgtggtct tttctcgtgc tttcattagt tattttgatg attgcggcag cattcttcgc
    21661 agatgatccg ccgcgttctt gcgtttcctg gcagcctgtc tcaggagaga tgtgtgttag
    21721 ttttggcagg tccacgttgg tcgtggtgtt cttggtgact gcgtcactat tggtgtggtt
    21781 attaatggtg cgctgggagg gcgagatcgc ctcgccgcaa caaaccgctt ttgctttgat
    21841 ttccctgtct atggcaaaca tcgcttttct ggctgaacat tttttgctgc gttatgttgc
    21901 gctggagttt gtgggtttgg gtgttgttgt agctgcctgg ttgttctcca cacccaggga
    21961 gaagacctgg tctgcggtga agtcaatttt cataaacttt cgaataggag accttggttt
    22021 actaatttcc atttttggta tgtattctat cagtggcaca tttcacatcg atcagaattt
    22081 aatgctcgcc actctggctg acccaaccat gcgtgctgtg cttattttgg gtttcgcgct
    22141 ggctgtttgc gtgaagatgg cggttttccc cctcgaccgc tgggttgacg cttgtgcgtc
    22201 attgcctcgc tcactgaaaa tttggtttac gagattgctg ttgcccgcgc tcggcgcata
    22261 tttgctgtat cgcattgctc ccttgttgct ttccggcatc gtgagtgcaa cctggattct
    22321 caccctctta tgtgcactct tccttattaa aacgttggtt ttttctcccg ctgttcgacg
    22381 ctcagatttg gagcgagacc tgctcatgtt ctttgcgctc tgcctgttgc tcttggctgc
    22441 ttttgtcgag caagaccttc tctgggcgct cctgctcctc tggttgttga tccgagctgc
    22501 ttttgccttc tgcacagggc aagatgaatc ttgttcatta ctccgccagt atcgaaaggg
    22561 cttgaccgtt tattttccat attttttaat atcaggattt gcaatgatcg tactttggtg
    22621 gatgttcagt cttcctcaac ctatccctcc tttccttgtt gcagtgtatc tgttgctttt
    22681 tggcatccaa tttctgcata tccagagatt tatgatagtt aatgaagatg aatcaggtgt
    22741 gagggttgag tcaaaaaaag tgaagcacgt cttgaaattg gacttcttca acttcttgac
    22801 cgctgttttc atccttgccg tcttttcagt ggtcattttt ttcgtgacca ggctgattaa
    22861 gggagaagag atatgggtgg tcccggttac cctggctttt caaaatgctt cgttctccgg
    22921 gggacacctt gttttgactt ttggagcgct ggctgttgtg gtgatgctgg caaacatttt
    22981 tgggaagttt gcaacgaaac tcacagcttt cagccatgaa aagttccatg cgttgcacag
    23041 tttctcaaaa aagatcattc cttccgcctc agatcgattg gacgtgagca acgctttgtc
    23101 ggcagcattt gtaaggattg ctacctatgt atacgaacat gtcgaacata acttgctgga
    23161 gaagttcgtt gccgtatttg caagggtatt caggtttttg ttcatgacgg tggaaaaatt
    23221 taccagcgcc gatctttgga agcatgctct tgattcggtt atgaaatctt ctcgtcgttt
    23281 gcagcgtatg catgcgggtc tattgcgtat taaccttttt tggttgtttt ttgtgcttgt
    23341 ggttttgatt gtttttgtgc ttgttgtgta tcgcggtgca gtgaggttgg tgggatgaaa
    23401 ggagacaaag ggtgatgaac acgcaagagt tgtgtaaggt atcagtggta ttgatcaaac
    23461 ttgttgttgc tgtattttta ttgttgttgg ggaaatatat cggcaagaaa gctaagtggg
    23521 tggcactagg aacatccgtt ttgtccactg tgcttttcct ggcgtattta cccgctttgc
    23581 aagccggtgt gaatacgccc ttcgatatag aataccttcc gttgctgggt atccgattga
    23641 gcttgcatat agattggttg tctttcccct ttttattgac ggaagaattc gtgacgattt
    23701 tcgctgtgat ctttgcgctc gattatgtaa agcaagacca tcgcactcac cttttttatg
    23761 ctttgctgct tgttttttcg gctggaatgt cgggcacaac gctggcgaat gatatatttc
    23821 tgttttactt tttctgggag ttgatgttgg tagcatcaac attattgttg ttgaattggg
    23881 gagatagcga gaaaagaaca gcaattgccc taaaatattt catcatcacc cacctgggat
    23941 cgttgctggt attggttgcg tttgttctga tctatgccca gggtgggata gatcatttct
    24001 cttcactgca ggcagctttt caaatgttcc ccgataagtt gcccttgatt ggctcgttct
    24061 ttctgattgg ttttggcatc aagatggcgc tggtaccatt ccatatctgg cttccggata
    24121 ctcattcaga agcgcctatg ccagtcacca taatgctggc tgcagccatg cttagcatgg
    24181 gcacctatgg aattctcaga tttccaatga gttttttcac catgcagcag atgctggttt
    24241 tctcgtttcc tatgatgctg gttggtgtga tctcggaaat atacggtgcg atcatggcac
    24301 tggtcgagac ggacattaaa cgcatcatag cttattcaag tgtaagtcag atggggtaca
    24361 ttttctttgg tctgggaacg ctaacctatg ccggtgtcac aggagccacc ttgcatgtta
    24421 tttatcatgc aattgtgaag gctttgctat tttgttgtgt cggttggatc atcaaagcgg
    24481 ttggagaacg tgatatcaac caaattaaag gattgggaaa gaccgcaccg ctattatgcg
    24541 cctgtgcgat tataggcgcg cttgcaatcg cgggggttcc ggctttggct ttgttcaaca
    24601 gtgaatggct gattttcagc agtggctttc aaacgcccta tctttcgctg gctatctttg
    24661 aggtgttagg ttcgctgttg acagtgattt acattcttcg ctttatagga cagtgcttct
    24721 ttgttcccgg tgaaccttcg aaagtaaagc ctgtgcccct ctcgatcaaa gttgccactg
    24781 tgtcgctctc tgcgttgacc attatcgctg gtttgttccc caaaccgatt tttgattatg
    24841 tggtcaatga attgccgtcc atattgggtg gaaatttgtg atgaatttga aaatgctttc
    24901 ctaccctcaa atccccctct tatttttggg agtcaccgcc gtgctgattt ttctgctggc
    24961 acgattttta cgcgttaaga acactgtgat ggcgttaata accgccggtg ctttcttatt
    25021 ggtgagtttc tatctacttt atgaagcgca ctggatacgt gttcttgaac tttgcacccc
    25081 taacttcccc aatgccctga cggaaatgtt tacggagggt gcttttactg ccaatgatcc
    25141 cgcagcagtg ttgattggcg tggtcgcttg tgggctggtt ttcttgattt ctttgtactg
    25201 tgtggagtac ctaaaattcg accgacgcca gcatgtcttt tatcctttgt tgatcttgat
    25261 gatatgtggt ttgctgggca tgttgtttgc ggatgattta atggttgttt atttgttttg
    25321 cgaacttatg agcatttgcg cctatgccct ggttgctttt agaaggaaga ttgacgctgc
    25381 catcgaagct ggatttaaat acctgatcat gggcagtgtt gcttctgtgg tcctgttgat
    25441 gggaattgcg ctgatattcc agtctgcggg aacggtggat ttatcgaagg tggaggatac
    25501 cagcggttta ttcgcccaaa tcggcatgat catgcttctg gcttcgttta gcctcaaaag
    25561 cgcactggtc cccctgcaca cctggctgcc cgatgcgcat gggcgcgcac ccagcagtat
    25621 cagcgcggtt ctttcgggcg tcttggtgca gggtgtgttt tacaccatgc tgcgcatctg
    25681 tcttgcgctc ggaatggatc gattcgtatt aggaagctat ttgcttgccc tgtcattgct
    25741 caatatcttg gttggcaatc tggtaggtct ggttcaaaca cataccaaac gcttgctggg
    25801 ttattcaacc attgcccaaa tgggttatat cggcctctgt tttgctttgg ggttgcgtaa
    25861 tcagtcattg cttgcgttgc aatctggttt ctttttgatt gttgcgcatg ccattgcgaa
    25921 aagtcttgct tttttggtgc agggggtttt ctactactat ctggatgcct ctcagatagt
    25981 agacctgcag cgggcaggaa gattgcccta ctttttctct ctggcgtttg gaatttccgt
    26041 tctaagcctt gcagctatcc cccctcttcc gggctttatg gggaagtgga tcttcctgac
    26101 cagcgtcttt gctcaaatcg atgcctggtc catggttggt gtaattgcgt tattggtcgg
    26161 tagtcttgtt tcactgggat attatttcaa gatattgcag aatctgtttt ttagacctgc
    26221 tcctccaatg acagccgaca attctgcgaa taaacagaaa gtatcggttt ggatgctgat
    26281 tcccgtagcg gggttggtct tctcgattgt gatgatttcc atcacacccc atacccttct
    26341 cgatgggaca ggaaacgcgg ctcaatattt aatggaactg atgaaataag atggataaca
    26401 cgactttttg gttctcccct cccgttttga tgatcgtttt tttgctcctc ggcgggttca
    26461 tgctgtttat tgggaagatg atttccgcga aagggaggca tagccgtgct aaataccttc
    26521 actattcctg tggggaagaa ctgaatcatt cttctttatc gttgaattat catgctttct
    26581 tccgtcttgc tttgttgttt agcatactcc acattgttgc cctgatcatc tccacaatgg
    26641 acccgaacgc cgatacgaaa ctgtatcccg tactttactt acttggggca ggggtaagca
    26701 tgttcattct tctggagcgc gatgaaaaaa aacagcatca aagtatccaa catcgtttct
    26761 tgggcgcgta agaaatcgcc ctggatactg cattttaata gcggtggttg taatggttgt
    26821 gatatcgaga tccttagttt gctgacccct ctctatgatg ttgaaaggtt tgggattctc
    26881 aaggaatctt ccccccgcca tgccgacatt ctgatttgca ccggaccggt caccgagcag
    26941 gtcaaaccgc gccttcagcg gatctacgat cagattcctg aacccaaatg ggtcttggcg
    27001 gtcggcagtt gcagttgcac aggcggaata ttttcgggct cttacaatat cctgggtgga
    27061 atagacaagt tgcttccggt tgacatgtat attcctggct gcccctgcag gcccgaaacg
    27121 atcattgatg gaattatcaa attggtggag ttgatggaat gaagatggaa gatattgtca
    27181 atgatctgat ggctaccctg ggtttggata accttcagac gatgttggga atccctgatg
    27241 atgaacaggt gattcacctt cctgctgcac aattattaac ggcggttgaa aaattgcttg
    27301 agctgggcgt ctggcatctt tcagccatta cctgcttgca ggaaaaatgc ttcatcctgc
    27361 tgtatcactt ttggtgcttt gggaacttga cgctacgggt ggaagttgca gaaggtaccc
    27421 cttgtattcg aagcgttagc gcgctgatac ccggcgctga gtattatgag cgggaaattc
    27481 gcgagatgta cggaattgag tttgagggtt taacgaatcc ctcctcgctc tttttgccag
    27541 ataactggca cgctggctac cccatgcgca caactgacga gcaaaatgca gttgccggtg
    27601 aaaatgaacg aaaaaacgga atgcaaggag gaacatcttg aacaaggttg tcgtaccgat
    27661 tggaccacag caccctttac taaaagagcc tatgtcattt ttgatatctt tggagggaga
    27721 gcaagttgtc gatagtgtgt tacgattggg ctatgttcat cgggggatag agaagcttgc
    27781 tcaacagcgc acttatatcc aaaatgtata tcttatagaa cgcatttgtg gcatctgttc
    27841 ccatattcat accaccactt attgccaggc agtggaaact ttgttgggtt tagaagttcc
    27901 taaacgtggg ttgtatattc gtacgctttt atgtgaattg gaaagaattc atagccattt
    27961 gctttggttg ggagtgcttt cgaaaaatat tggcctcgat actatcttca tgtatgcctg
    28021 gcgcgaccgt gaaatcgttc taaatctaat ggaggagata tcaggaggaa gagtatccca
    28081 cgctgtgaat gtgattggtg gggtgcgtgt tgatttggat gcaatgcata tagccacctt
    28141 gcagaatggg cttgctgagt taaacgatcg tgtggaagaa ttgacccgct cgatcgaaca
    28201 tgatcgcagt tttgcggcac gcacacgtgg gataggaaaa cttactttgg aggatgtcaa
    28261 acgtttttgt gttgttggac ctgttgcgcg ggcgtctggg ctcaatattg acctccggcg
    28321 tgatgcgccc tatggtgcgt accgtgattt tgagatggag gttcccatta tggatcaggg
    28381 tgatgtatgg gcgcgttcca tggttcggat gtatgagacc gtgcaggcga tccgtctatc
    28441 cagaaggatt gtggaagctc ttcctgaagg cgctgtttcg gtcaaagctc ctcgaaaggt
    28501 ccctcagggg gagatcgtta gccgtgccga agccccccgc ggagaagtgt tttactatgt
    28561 gcgcagtgat gggactcaag tgccagcccg cctgaaagtg cgcacgccca cgcttaccaa
    28621 tctcatcacc ctttccaaac aattgaaaga tgttcaaatg gcagatgttt catcggtgat
    28681 tgcgggggtt gatctgtgta ttgcttgtgc ggatcgctag acaggatacg ggagcgctca
    28741 tgaatgtgct gcaagccatg tttcagattt ttatttttcc agggtttgtt tttctgtttg
    28801 tttcagcgtt gttttttcat tggttggacc gaaaactgat cgctcgcttt caggggcgaa
    28861 ttggtcctcc ctggtatcaa ccgctggctg actttcttaa actcttcgca aaagaggatg
    28921 ttctacccca aggaaccaac ctgcttttcg cgtctttgtt accgttagta tcttttgcat
    28981 ctgtgctgac agccagtttc tacttgcccg tggctgtgct cactgcgcct tcctttcagg
    29041 gtgatttgat tgttgtgctg tttctgttga gtatgccctc tcttttatat ttcctggctg
    29101 ggtggatcac tggcggcgta tattctatcc ttggtgggat tcgttcgttg ctgcaatatt
    29161 tttcttatga aatccctttc ttattagcgg ttagcggtac ggctattgcc agtgggtcat
    29221 ggtctctggc agacattcgc gcagcccagc tctcttctgg tgcatacatt attccgcaat
    29281 ggtttggatt ccttctggct gtttcaggct tgattggaaa actaaagcgt gagccctatg
    29341 atatacccaa agcccagtcc gagatggtgg caggtccttt gaccgagttc agtggcaaaa
    29401 aattggggct ttggtatttg accaaccatc tgcagacggt agtgggtctg gcatttttgg
    29461 ttaatgttta ttttgctggg tattgggaga tgaacacgct gggtgggttc atcatttgtt
    29521 atctcttgct gattttactg caatgtcttc tgtctgctat tagtgcgatc tatgcgcgct
    29581 tgcgcatcga ccaattaatc agtctgaact ggcgtgtgct cattcctctt actcttttgc
    29641 acaacctttt cttattattg aaatgaaaac tcttatgaaa ttgcgttctt tcttttactt
    29701 gctgccgaat atcatcacga atattttctc aaaaccgaga acgatgaatt accccaccga
    29761 acctgctgcg gtttcagtaa attttcgtgg gagtgtgcgt gttcattcat ccaattgcgt
    29821 tggatgtagc ctttgtgtgc gtgattgccc cgccttcgct ttagagcttg aaggggaaac
    29881 caacgggcgt ttccgtctga tccattaccg tgaccgttgc acctattgcg gtcaatgtga
    29941 attgagctgt cgttataaag cgctgtatct ggaccatgct tatgatgagc cctcttcaga
    30001 ccgtcgaaat ttcatcattg ttctcgttga tcatatggct gaggattgaa agcgcagggt
    30061 atctgctcgc tgcccccctt gttctggagc aataatttat cagttacccc gcataagggc
    30121 agaggtggtc gctccacaaa ccacctctgc ttgtttcaga attaatgaaa aacgtttgga
    30181 ctgtatctgt gctgccggat agatgctatt ctctcgccat caattattct gcgccccctg
    30241 tgcgatccaa tcatagaata actggatggt ctcatcgggc aacttcccag tgggtggcat
    30301 gacgggcacg ccatcaccgg tcagatattt atacagcagg ctggcttcag ggtcacccgg
    30361 cactacgtac cgcatgatct gatcatagct ttccaattgc actccgccct tcatttcaga
    30421 tccgtgcgcg gggagggcaa actgttcaat cactggccag atatcgttgc tgaaactgac
    30481 tacattttca tcagcagtat caacgacttc actatccgcg ctttgtacgt cggtcgcttc
    30541 gctggtatcc atggcgggtg cggtagcggt ttctacggct gtcccgctgg cacaggcgct
    30601 caaaatgagc gcgccgaaaa cgagcaaaga taagaatcgt attttcatat cgttctccca
    30661 ttcttgtttt caaaatagta caaggtctga acaggataaa atgtatgata attgtaagaa
    30721 agggtgggca tttctgtttt tccctactga tctctggcgc gattggaaca gctactctgc
    30781 gtgtggcgtg aattttttgg aagagcggcg gcacagcggt ggtctttcag gagacctgta
    30841 cggcgaccgg tatttgattt ccgatcaaca tgtatctttc tgtggcttac gaatgcccga
    30901 acggcaaacc cgctggtggt caccagagat gcgatccatt aaagaagatg gacgttcctg
    30961 cgttcgagtg gagtgctgct gaatccagtc ctcactctct tatggatgat gaatttacgc
    31021 caatttacgc cataaatttg tcttggcaga aaagatgatg tataatttat ttttgtaact
    31081 tgttgggtta acccctcatt ttgagaggag aagtatgccg catatttccc gtgtaagtta
    31141 taagccaatg tacctgcaga tctgtgagct tattcgccaa aaaataaact caggagagat
    31201 cgccattggc agtcgcgtgt ggtcagagag cgacatcatg gagaaattcg atgtcagccg
    31261 caatactgcc cgcaaagcta tcgaaacgct ccaaaatgaa ggaatcgtat cccgcataca
    31321 gggtaagggc tcatttgtca gtcaacccat ggttaattat gggctgcaga acttgatgag
    31381 tttttccgaa gagacccgag aaaagcggtt aattccgacc tcaaaggtga tcgccttcac
    31441 cagagagcat cccgatgcaa cccgtgcaga aaatttaaaa atacttgaag atgattgggt
    31501 gtttcgattg gaacgcgtcc gcaatgcgga tggaaatcca atcgcttacc agattgcttt
    31561 tctgccggaa aaactatgcc cggggttgct taactatgac ttcgcggaaa gatcgttgta
    31621 cgacgtgttt gaaacggatt acaaccatgt tctttcctgg cagaaaaccg tcatcaagcc
    31681 catcgtggct gatgattacc tcgctggcat cctaaagatt tccccccgca cgcctttgct
    31741 gtataccgaa tcggttacct atctggcagg aggaacgcca attgaatcga gcaagaatat
    31801 ttatctcagt gaacgctatc agttcactgt gctttctcat cgatttgacc cggcaagggg
    31861 tgctgaaaac aaatgaaaat aggcttgatt ggcagcggtg ggagagaaca cgccatcgca
    31921 aatgtattgg gtaaatcacc ttcgcacgat gcactgtttg tctatgggga ccatcacaat
    31981 ccgggaatcc aaaaacttgc tgcagctttg accatcgcat ctatgcagga tgtgcgcaag
    32041 atcgttgaat tcttcactgt ccatcaagtt gaacttgttg tcgtcggtcc tgaggttccg
    32101 ctgatcgccg gagtgagcga ccgattgcga gcgcagggga tcaaagtggt gggtcctgct
    32161 caggcacagg cacgcctgga aggggataag gtctttatgc gcgatctttt gcgtcgtcgt
    32221 gtgcaaaaag ggtcaccacg ctggaaagaa gtgaaagatg ttgaaactgc ccgtaatttt
    32281 atccgggagg tcggtcaggt ggcggtaaaa ccgatcgggc ttaccggagg aaagggcgta
    32341 agcgtcatgg gggtgcactt ccattccatg gaagaggcac ttgccagggt cagaagttct
    32401 cttgagcaag acggggttgt gctgctggaa gaacgcttga ttggtgaaga gttctcgcgc
    32461 atggcatttg tgtatgatgg aaagctgatt ggcatgccgg ttgcgcagga tttcaagtat
    32521 gcgtttgatg gcgaccaggg agggatgacc ggaggcatgg gtgcctatac catggcagat
    32581 ggaaccctgc catttctgac ccaagaagac ctgtacgaag ccgattcgat cctccaactg
    32641 acggtacatg ccattgagga ggagtcgggg caaggatatc gtggcttctt gtatggtcaa
    32701 tttatcgcaa ccgcacatgg ggtgcaggtg attgaataca atgtccgcct gggcgatcca
    32761 gaagcgatca acatgatggc tttattgaat acggatgcac ctgctttctt ggctgacctt
    32821 gccaacggtc atttggacga gagcagcacg atattcgccc ctgccgcatc tgtgtgtaaa
    32881 tacgttgtcc cggctgccta tccccaatca gggaatgagc acattcgtgt tgacttgaat
    32941 gagaaaacga tccgacaaga aggtctgtcg gtggtctatg cttcgatgga agaaaaggct
    33001 accggtcttg aaacgttggg ggcgcgggct tttgccatcg ttggaatagg agaagacgcg
    33061 ggtagcatct cgaccaagat agaaaatctg ctcgagcgca tagaacctga aaatttgcgc
    33121 catcgtaaag atgtcggcga tccgaagatc atccgggaaa agatccgaaa aatggagaat
    33181 ttgcggagaa agaaataaca tgaaaacagc gaagaccgag attacagccc tggaaagatt
    33241 ggtggaaatt cctggactgc cgggaaggga aggacgcgtt gctgatttca ttcggtcctc
    33301 cttatcccct gcctgcgaat gttgttccga taatttgggg aacctggtcg cgcatatccc
    33361 tggaaaagga aaacgtgtga tgcttgtggc acacatggat gaggtggggc tgatcgttca
    33421 gcgcattctg cccgatggat ttctgaaagt agaacggatg ggtggaacca gtttgcgcgc
    33481 cttgccgggc agtcgcctcg ccctgtggac ttccggcggg tgtatcccgg cagtggcggg
    33541 aattcttccc cagcacctgg atagcggtga acccatttcc gatttctctc gcatatatgt
    33601 tgatattggc tctgcatcag aagaagaaac gcgtgcgatg ggagtgcgac ccggtaatgg
    33661 tttaacttgg gattctccat tgcgatttct caacgcttca ttgatctgcg cgaaagcgct
    33721 ggatgatcgc ctgggttgtt tctgcttgat gcaactggaa aaagtcatca aaccggatga
    33781 cttgaaatgc gatctttacc tggttttttc ggtgcaggaa gagaccatgt tgacgggtgg
    33841 aatgcccgta gccaatgcga tcgaaccaga ggtgctgatc ggggtggatg gcacgttggc
    33901 ttttgacacg ccggatctgc aaggaaaaca aagtgatatt cgcctggggt gcggaccagc
    33961 ggtgaaatgg atggatgcca tccgcgggaa aatggcggct tttgtacccg atcaacgctt
    34021 gaccgaattg gtgtgcagaa ccgcacagag caatggtatt cccctgcagg atgagattgc
    34081 ggtgggaatg agcactgcca ttacccctat ggtgtacgcc tctaagggat cgcctgcgtg
    34141 tgcgatttcc attcctattc ggtatcatca cacccccgtt gagaccgctg atctgggcga
    34201 tgtagagcat gcggttctgt tgttaaaaca gttggtggag attgaccttt aaatgacttt
    34261 tacggcattg tagtgcagtt ggaacattaa accgaaatat cggttggtgg cttatgccca
    34321 atttcatctc attgttcacg catagatcaa tgaaaaggag gtgctgcgga aagatatata
    34381 cgacggatgg aaagaatgat gtagaaaacc aaaataaaca aaatattgtt aaggagatac
    34441 aatgaagagc aaacttttca cattcttcgc tctgttgatc gcagttggta tgttgttaac
    34501 cgcctgctca aatggtgccg aagaagcccc cgcatctgaa gaagccgttg cagaagcaga
    34561 atccttgaag gtcattcatc tggtgaatgg cgttctcggt gacaaatctt tcttcgattc
    34621 cgcagagcgt ggtatcaaaa gagcatctga agagttcggt tttgagtata aaaccatcga
    34681 aatgggaacc gatccgaccg tttgggagtc tggtctggct gatgctgctg ccaacgaaga
    34741 ctatgacatc atgatcatgg gcacttacca gatgatcggt tatctggaag ccattgccgg
    34801 aaactacccc gataaatatt tcatgatcta cgattcagcc gccacgccag aaaactgctc
    34861 gaatggctgc gcgaatgtgt attccatcac ctacaagcag aatgaaggtt cttatgtggc
    34921 aggtgtgtat gccggtttga tgacccagac cggcatcgtc ggtgtggttg gtgcccaaga
    34981 tatccctgtg attaatgact tcatcgttgg ctacacacag ggtgtgaaat ccgtgaatcc
    35041 tgattaccag gttctgttga actatgctgg tggttggaac gatcctgcca aggggaaaga
    35101 acaagcgttg gctatgtatc agcaaggggc agactatgtg ttccaggttg ctggagcaac
    35161 cggtgacggc attttccagg ctgctcagga aaccggcaat tacgccatcg gtgttgattc
    35221 ggatcaggcg acgatcatca aagataccaa tcctgaattg gctgctgtga tcgccacctc
    35281 catgatgaaa aacgtcgata acagcctcta tcgcgctttg acgatgcacc tggaaggaac
    35341 gctcacctat ggaaccagcg aagccctcgg catcaccgaa ggtggtgttg ggatcgccaa
    35401 gaatgagatc tatgaagcgt tgaccccctc agaggtcaaa gaccagatca cccagataga
    35461 agctgatgtt gcatcaggga aaattactgt tgacacagcc ttctaaagaa aaataaaata
    35521 ggaaaaagta gacagaggtt ctgcaacctc tgtctaccct ctcttcgaat cgtggaggca
    35581 agatggcacc gcttgttgaa atgcgtaaca ttgtgaaaat atattcgaac ggcgtgattg
    35641 ccaacaatgg tatcgatttc cagatcgaaa aaggggaaat ccatgccatt gttggagaga
    35701 acggagcggg gaaaacgaca ttaatgaaga ttttatatgg atcagaacag cccacgtcag
    35761 gtgagattct cattaatggt gaaccggtga ttttcaaaaa tccattggat gcgatgaaaa
    35821 aaggcgtagg catggtccat caaaatttca tgcagatccc ttcttttacc gctgcggaaa
    35881 atgtggtgct ggggcatgaa cccacccggg gtatcagcct tgataagaaa aaagcagttg
    35941 aagaaacccg gaagttatca gagcagtatg gtttgttggt ggaaccagag gcgatcacgg
    36001 aaacgatcaa cgttggaatg cgccagagga tcgagatcct caaaatactg taccttggcg
    36061 ctgaaatatt gatttttgat gaacccacgg cagttctgac cccgcaagaa acccatgatc
    36121 tgtttaaggc tgtccgtgtt ctgatagaaa aggggaagac catcgttttc atcacccata
    36181 agttgcgcga ggtgactgag atatctgatc gcgtcactgt catgcgtaac ggcaagatca
    36241 tgggaacttt gaagacagcg gatgccacac gggaagggct ggctcgcatg atggtggggc
    36301 gtgaggtttt cctggatatt cataagcaac cgctgaaacg cggtcgaaaa gtgatgtcta
    36361 taaaagatct ttcctacgtt tccgaaaccg gacgtactat gttgcgggat ctttcattca
    36421 acctgtatga aggtgagatc ctgggcatag cgggcgtgga agggaatgga cagacggaat
    36481 tggttgaggt gctcagcggt ctgcgcgccc ccgcttcagg aagtgcgctt gtgggtgaaa
    36541 cggtgatcac tgcgaaaact ccccgcgagg ttcgccaggc tggtgttgcc catattcccg
    36601 aagaccgctt gagcaatgga ctgtctttat cggcatccat cgacgataac ctgattgttg
    36661 atcgcttttt caaacctccc tttaagaaag gggtgatcat tgatcataaa gtggttgagg
    36721 ataatgggaa aagattgatc agacagtttg gaattctcac cccgaacggc gaattgccgg
    36781 tatcttctct ctcgggtggg aatatgcaga aggtcgttgt tgcgcgcgaa ttctcatcaa
    36841 acccgaagat tttgatcgct gcccaaccta cccgtggtat tgatgtgggc gctacggaat
    36901 tcatccgcga ccaactggtg caaaagcgca cggatggaac tgcggtcttg ttggtttctg
    36961 ccgatctttc tgaggtgatg agcctttctg accgaatcat caccatgttt gaaggtcaga
    37021 tcaccggtgt gtttcccgaa gcctccaaag ttactgagga agaattgggt tattacatgt
    37081 taggcataaa gcggcaaact gctgaagaga tggaggcatt gctatgaaaa aattcttttc
    37141 caatcccacc atacgcgaag ggttgcgcat cctcctggca attagcagcg ctctcgtgct
    37201 tggtttcatc attactctgt ttgtttcaga agatcccatt ggcgcttata gggcatttct
    37261 aaccggaccc tttaccagat tcaaccgctt cggagattgg attgaggaat ccatcactct
    37321 gatcttggta ggattatcgg tcgccatcgc tttcaaagcg aatatgttca gccttggcac
    37381 acaggggcag ttggttctgg gtgcgctggt ttcgggtgtg atcgcgttat atgttcctct
    37441 ccccactatc ttaagggttc cgctcgccct cgtctgtgct atggttgtgg ggttcatttg
    37501 gggttggatc cccgggtatc tgaaagcgaa cctcgatgcg aatgagattg tatctacctt
    37561 gatgctgaac accatcgcca ttaagcttta tgattacttg cttgtgtttt atataaaacc
    37621 aaaggatgcg ggctataacg tttcggcgcc ttttccggta gaagggcaat taccttcatt
    37681 tgtccccaat ctgccattcc tcaataaagt gtataccctg ttcacaacca acaccaatat
    37741 caccattatg ctgtacattg tagtgctggc tgtggtcgtg gcgtattatc tggtctttaa
    37801 gaccccgttt ggctataaat tgcgcatgat cggaagcaat atgaaattcg ctcgctatgg
    37861 tggcatcgac acgaaaatga cgaccatcct ggtgttcgcc atcagtggcg ttttcggcgc
    37921 tctggcgggt gcgcatatga ccatggcgat ccaacactcc attattacca atatatccgt
    37981 cagcatggct tttgaaggga tcattgtggc gatactggcg cgcaacaatc cgctgtacat
    38041 tccgcttacc ggtctcgctt atggctattt acgcgcagga gctaatatca tggagcgttc
    38101 atccgatgtg tcgcgcgaga tgatctccat catccaggcg cttatcattc tgttcgtcac
    38161 agccgagcgc atccttccaa cggtccaaag ggtgatcgaa gaaaggaaag aacagaacgc
    38221 aaccaaagaa agatccctac cagcgggagg ttccaatgaa cattaatctg gttttgcaag
    38281 gaattttcag tgcgcttttc atcgccagtg ttttgcgcat cagcactgct ttggttctgc
    38341 ccgccctggg tggcttgatt actgatctgg cgggttccat taacatcgct ctcgaaggaa
    38401 ccatgttggt ttctgccttc accggtgtta tcgtgtctgg tttttcgcgt aatgtctggc
    38461 tgggagtgtt ggcgggcatc gtctctggtg tggcattttc agctttactg gcgtatttcc
    38521 atctgtatct gggcaccaat atcattctgg ctgggaccgc tttgaatatc cttgcgtcag
    38581 gcggaacggt tttcctgttg ttcatgctta ctggtgataa gggcagttct tcaacgctgg
    38641 cgagtggtgt ggtgccgacc gtgaacatcc ctttcatcca ggatattccg ttcctgggaa
    38701 ccgtcctttc agggcataat gtgtttgtgt atggcgcttt tctgcttacc ttcctgatca
    38761 ataaactgct gtacaatacc aaattcggag ttcatctgcg cgccgtgggt gaagatccgc
    38821 tggcagccac atcggtcggg atcaatgttc gtaggaccaa atttttgact atcctcctta
    38881 gtggttttct ggcatccctc ggcggattga acctttctat ggcgtatcta tcctctttcc
    38941 agcgcgatat gactgccggg cgtggtttca tcgccttggc tgcagtatac ctgggaggaa
    39001 gaaaaccttg gggtaccgta ctggctgcga tcctctttgg cattgccgat gcgctctcta
    39061 accagttggg aacgctgaag attccttctc agttggtgca gatgatcccc tacgtcatca
    39121 ccgttctggc gttggtggtc aacggtatcc gcaatcgcac ccgcttgatc gaacagacca
    39181 aacgtttcca agaacgcgag ttggtcaggg cgaacagcga gaagccgaaa taaccggaac
    39241 cggttgtact gtacctgcgc tcttatttgc ctggttgcgg atatacaata gaaagaagga
    39301 gaaaaagaaa cgatgatcat ccaaatctat gcgttcaccg atgttgacac tgccttgaag
    39361 gcagcggaat tcggtgtgaa ccatatcggt tttgtagccg ggaaatacgg tgaggttccg
    39421 gcagaactga ccttccggga agcgcgggaa attgcggctg cactgcctcc gcaagccacg
    39481 gctgtagcac tcactatggc tactgaggtg gacgagatct tgcgcatggt ggagcaggtt
    39541 aacccccata ttgtgcatat atcttctgat ctggatgacg tggacgtgga aaccatgcgc
    39601 gagttgcgct cgcggctcga taaaaatatt cggttgatga aagccatacc ggtagaagat
    39661 gaaaccagcg tccaggtcgc acagcaatat gctgcggtga gcgacctgct cttgctggat
    39721 accaaacgga gcggtttccc cggcgtggga gcaacgggct ttacccacga ttggaatatc
    39781 agcaagcaga tcgttgaaac tgctggagtg ccggtgatcc ttgccggtgg actaaccccg
    39841 caaaacgtgg cagcagctat tgccaccacc catccctggg gtgtggatag caatacctcc
    39901 accaacgttc ctggcagcaa tacggataag gatcttcaac gcatcgctga ttttgtaaag
    39961 gctgtccgtt ccggagaaaa tgaagcatga gcagcggtga ggtcctgctg ctgggagaca
    40021 tcaatattga tactgtcttg cccgtagccg aatttcctat gccgggtaga gatggattgg
    40081 ctccgcacat atctaccggg attgggggtg cggttgtcaa ctctgctttt gttctccaac
    40141 atcttggtca gagaacggca ttgttgggct gtgtcggggc tgacgtttgg gctgaaaagg
    40201 tgaagcagga tgtggggcag acgaccatca aaatggatta tgttaaaagt aagccacagc
    40261 attgcaccgg gcttactttt attatcgcca ctcccgatgg cgagcgcacc atgttctcgc
    40321 atcgcggtgt gaacacccaa ctggcgccgc aggatattga tgaaaaggct tttcctgggg
    40381 cgggcatgct gcatatttcg ggttatgctc tgctggaatc tcctcagaaa gaagcagtat
    40441 ggcgggcggt ggaattagca aagcagaatc aggttcccgt tagtttggat accggattag
    40501 accccgttgt gcgaaatccg gacgaggttc gtcgattgtt gcccgctctc accctgtgca
    40561 ttaccgggct caaagagatg gaaattcttt ttggagacat tcctttaagt gaagcggtcg
    40621 atgcaatgct tgcatccggt gtccggctgg ttgcgatcaa attgggtgat aaggggtgcc
    40681 atgtatttgg tgaagcggaa aagatttctt atccagcttt tcaagtgaat gcgctcgata
    40741 caaccggcgc cggcgattca ttcacctcgg ggcttatcta tggctggttg cagggcatgg
    40801 gtctgttgcc tgcagccgcg ctggctagcg cactgggagc gcttgccact actgtgtacg
    40861 gagccggatc tgcgttgccc gctaaaggga aggtggtgga ctttttaaga acggtacaag
    40921 atcaatctat cgcaggggaa atcgaagaat tgctgggtat attggaaaaa aatacttgat
    40981 gtgtgggtag cgatatatcc acgttctggt tgctgtcaaa agatctttat agcgaaatgt
    41041 gaggttcaaa acgaggagag tatgtttgca gagatcaaag tgaatacgga aaaaagtgaa
    41101 tgtctgatga atatcaccca ggaagttcaa caagctgtca cggaaagtgg tgtgaaagaa
    41161 ggcgtttgtg tggtttttgt tcctcacacc acagcggcgg tcaccatcaa ttcctgtctt
    41221 gatagcatga cagccgaaga tatcaccggc gagattcacc gcttggtgcc aacccgtgta
    41281 gatttcaagc atatctacga tacgccgaca gatgctgccg ggcacataaa atcggtgctt
    41341 gttggcaaca gtctttcgtt gattataaaa gatgggcggt tgttgctcgg cggttctcaa
    41401 agtatcttgt tctttgagtt cgatggtccg cgcaaacgcc tggtgcaact gcgcatcatg
    41461 ggagatcctg actaaacggg aggacacatg ccacaaaaaa tcattattga caccgatcct
    41521 ggggtagacg atacgatcgc catttctgtt gctctccgct cacctgagct ggaggtggtg
    41581 gggctgacca gcgtatttgg aaacgcgacg acggagatca ccacgcagaa tgcgttgcgt
    41641 ctggtggaat tggaagctca cgattcggtc cccgttgcac ggggttgtga tgttccactg
    41701 gtggtggagc tggaagcgat tgcttccgaa gtgcacggca aagatggttt gggaaatacc
    41761 tatttgcccg caccgaaagg aaaaccgatc gcgaagaccg ctgcggaatt catcattgat
    41821 acggttcaca aatatccggg cgaggtggtg ctggtgcctg tcggaccgct gacgaatatc
    41881 gccgtcgcct tgaaagccga tcctgaaata gcgcacatgc tcaaaaagat cgtgctcatg
    41941 ggtggggcgg tatccagagc tggcaacatt tcaccagttg ccgaagccaa cgtctaccac
    42001 gacccgcacg cggctgatat tgtgatgcaa gccggctgcc cggttgtgat ggtcggtttg
    42061 gacgttaccg aagaagtggt catgacccct caattttttg aagagttatt ccgcgcgaag
    42121 aatcccgctg ttgatttgat ccggcgaatt ttgcctgtct atcaaaatta ttttgatcgt
    42181 tttcttggga tgaaaggaag cattcatacc catgacccat ccgcaattgc ttacttgtta
    42241 gaccccgatc tatttcagac cgcctccgtt cccgttttcg tggaaaccgt gggacgctgt
    42301 aaaggtcaaa ccgtggctga ttggcaccgg caatgggaaa gccgtgccga gatcgaggtt
    42361 tgccagtcgg tgaattccgt cggggtgctc gccctcatca aagaccgttt gacccgctaa
    42421 gagcgtgatt gaggagaaac ctgtatgcgg aacctttgga tcaatgtgag cgcggttgac
    42481 ggaacagccg ggtggaagac tgatgttcag cggtggaagg gtcgcttcta tgcagccttg
    42541 ccatgagcgc aagatcgacc tctattgtct gggcggtgcg tctgttgatc tcgttctgga
    42601 agtcccgcgt ttgccgctgg cgggagagaa gctgcttgcg cactattacg ggcaactgcc
    42661 gggtggtttc atcgccaata ccgcctgcgc ggctgccaaa ttgggattgc ggaccgcctg
    42721 ggcgggatcg gtcggtgatg acgccttcgg gcatagcatc atgcggggct ttacaaagtt
    42781 cggggtgcga accgatgacg ttgagctgct gaaagacgcc gcttctgatt ttacggttgt
    42841 gcttgttcac cccgatggtg aacggaccat cctgatcgtt cccagcctgc ccattccgcc
    42901 agccttggat gaacacatcc gttgctcttt gcagaattca cggattggct atactgtgct
    42961 ctatgaccat gattggttta tggaggtggc ggatcttctg catggcagtg gcggaaaggt
    43021 ggcggttgac ctggagctca ataccctacg tgatcgcgag gcggcgagcc agatgctgaa
    43081 agcggctgat tatgtgttct ctgataagaa tgctttgcgg gaattgaccg gaaatgctga
    43141 tgagaacgaa agcgtcaagg agatcctggc attgggagcg gagctcgtca tccttaccaa
    43201 ggggagtgag ggcgctgctg cttatacagc caccgggcgg tattccacag ggagctatca
    43261 ggtgcccatt aaagatacca ccggggcggg tgattgcttt cacgctgccg ttcttttcgg
    43321 tattctgtcg gatttccaat accaggattt tttggatttt gccagtgccg ccgctgcaat
    43381 cctgatccag gagatgggtg cgcgccaggg gttgccaacc gccgtggaag tacaaaagtt
    43441 tatggaaaat catatcagag gagatgagtt gaaatgaatt acatgatcga gcaaattcgc
    43501 agtttaccgg aattattgaa acagatctat gagccgttgg atgacactat tcggcggaag
    43561 gtcgaccatg agttgtgcct ttcggcaaaa cgtttgtacg tggtcggctg cggtgattct
    43621 caccatgccg ctctcagcac ggagtttgcg tttgaaacgt tgaccaagtt gcccgatgat
    43681 cccatgccct ccatgttgtt cgggcgctat gctgctgggt tgatcccgcc aagcggtccg
    43741 aagaccaaca tcgtcatcgg catttctgtg tcaggagctg tctcgcgcac tgcagaagct
    43801 gtgcgcatgg gcaggcaggc gggtgccaca accctggcgt tgactgcaac ccccggcagt
    43861 ccgctggcaa aggaagcaga tgtgatggtg gatgtgcccg ttccgagctt ccctgatccg
    43921 gtgaatatgg tcgtccccgg cgtgcgcagt tacttcacca gccaggtggg tctatacctg
    43981 ctggctatcc gttttgccga agtaagagga acgttatcca atgcagaagt tgcggcacac
    44041 cgggcaaagg tacacgactt caccagcgcc atcagccata ccattgaaat gtgtgatccg
    44101 cttgccagac aggcggcgca ggcatggaaa ggggatgaag agtttgtgtt cgctggtagc
    44161 ggtcctaatt tcgcaaccag tctgttcgtg gcagccaagg tgctggaagc gtctggcgat
    44221 tcagcgctcg ggcaggatgt ggaagaatgg gcgcacctgc aatattttgc caagaaagct
    44281 cccactccca cctttattat cagcgctgga gatcgcgacc ttagccgcgc tgcagaagtg
    44341 gctgtggcag ccaggcagat tggtcgcaag gtggctgtga ttgccccggc atccgcaacc
    44401 gaactggtta aattagctga ttatcacttc ccgattccgc aggtggatga gatgttttca
    44461 cctctcatca ccgccatccc gggtgaattg tttgcagcct acctggcgga agagaaaaag
    44521 gaaccctatt tccgtgattt caagggcgga cgtgatgtgg aaggtggcgg gggcattagc
    44581 cgcatccgca ccagtgaaac ttgggagcag tggcagtgat gtcggataaa caattgtttc
    44641 cttcggtgaa aggagcggcg attattgccg gtctccattt acctccatcg cccgcgtcta
    44701 accagaccac ctccgttcca atggactcga ttattgattt tgcattgaag aacacggaga
    44761 aagcggtaag ggctggggtg ccggctctct acatacaaga tctggctgat ctcccggtcg
    44821 cacccactgt tcaagctcat accgttgcct ttctgtcagt ggtgggggca gccatccgca
    44881 aggaattccc gcaactggtt ttaggcgttt gcatgatggc gcatggcgcc cgcgaaccgc
    44941 tggcggtggc gcaggctatc ggtgcgcaat ttgtgcgcat caaagtgtat agcggcgcta
    45001 tggtgaaagc ggaagggctt ctgcagggtt gcgcatatga tgcgatcacc tatcgcaaga
    45061 tggtcggtgc agaaaatatt gccattttgg cagatgtgta tgatcgcacc ggaaccccct
    45121 tgggtgaatt gccgattgcc gaagcagccc gttttgcggg tgtgaactgc aaagccgatg
    45181 ggttggtgct gaccgggctt tctttcacgg aatctctcag tatgttgcag gaggtccaaa
    45241 aagcaaaact gggcattccg ctgtttctgg gcgggggtgc cagagcggag aacgttaaag
    45301 aagccctcaa attctgtgat ggtgtcatcg tcagcagttc tttcaaaccg gtgggtggat
    45361 ggaccgaaga ggctatcctc gcagaatggg attatgagcg catcgcccgc ttcatgcagg
    45421 ctgtgaacgc ttagtcgctc agcgctggtt gaatcttact gaagggttga gaatgttcat
    45481 ttcgatcggt tacgtcatca ttgatgatat cgttctgcca gacggaacaa agcacatggg
    45541 ctgcctgggc ggaggtagcc tgcacgctat catgggtatg cgggtctgga gtgatcaggt
    45601 tggcttggtg gggtttgtgg gttctgattt tccatcagac ctgagggatg atctcagcaa
    45661 gctatttgat ctgcgcggtt tgaccacgct tcaggcgaga actatgcgcg cctggcagta
    45721 tttcgaggcg gatggcaccc gcaatgaaac cttccaaacc gatcccaatg aatttcccct
    45781 gttccacccg caggttgcct ctttccctcc cgattacaac cggttgcagg gtgtgcattt
    45841 gcattgcagt tgggctgagg tcgccgaatg ggtggaatat ctggaaggtt tggggaaccc
    45901 cttcatcttg tgggaaccca ttcaagcgga gttcctgcca gagaatcgcg acaatttcct
    45961 gaagattctg ccgctcgttg attgcgtttc ccccaatctg caggaagccc aagccttgtt
    46021 gggcgcgcct gatgcgcgca accaactgga tatgctcgtt cagaatggcg cccgcatggc
    46081 ggtgatccgc gccggtgcgg cgggcagcac ctacgccgat tctgaaggaa attatatccg
    46141 ggtgcccgcg gtgaaagttg aaaaagtggt agaccaaacc ggcgccggca atgcctactg
    46201 cggagggctg attgttggct gccagcaagc tgatgacccg cgcgaagcat tatgtcgcgc
    46261 tgcggtttca gcatcgtttt ccctggagca gttcggagcg atctttccgg tgaagggcat
    46321 tgagaaagca gctcaaagac gccttgatga gtgcagagaa gagctgtacc gcttgaatgg
    46381 gaattgagag accattccgg tttatataaa aaagcgatca ggcagccgct gaaaaaacgg
    46441 gatcaggcgg cgttccattt acttgcgcgg ttggtgggcg atgctccttg cgctgatcaa
    46501 aagggtactc accagaatgg caaccagcag catggatact cgcaaagaag tggcttcagc
    46561 caccgcaccg atcatggggg ctgtgatcaa tgagccggtg tatccaaagg ttgccacgct
    46621 ggcaatcccc acgcccgatg gaatgcccgg gaagttcccg gcagcgctga aagtgagggg
    46681 gatgatgttt gccagcccca atcccaccat agcaaaaccg gcgattgcca cgaccgggtt
    46741 ggcagtgaga ataatggcaa ccaatcccag cccggcagtg atactgcctg cccgcaccag
    46801 gttaaccggt ttccatttgc gcaccaggtg atcaccgctc aaacgaccga cggtcatggt
    46861 caatgaaaaa gctgcgtatc ccagcgccgc caacgaactg ctggtggaaa gcacccggct
    46921 caggtacact gcgctccagt ccgtcatggc ggcttctccg atcaccgagc aaaaagcaat
    46981 ggcgcccatc gcccacaagg cgcgttccgg aaagcgaaac atgctccgcc tctcctctgt
    47041 ggcttgcttt ccgggttgta gataggggta gaagacaacc gtaaccgtaa tgaacaatgc
    47101 agcaaccagc ataaaatgtg ccagaatcgt gacagccggc agagaagcca cccccgcact
    47161 caataacgcg cctgccaaac ccccgatgct gaagccggcg tggaatgaag acatcagcgc
    47221 ccttccgttc ttgcgttcca cgcgcactgc ctgctcattc atggcgatgt ccatactact
    47281 catggctgcg ccatagatga ataatgtgaa gaacagtgaa attggatccg gcgtgatggc
    47341 gatgagcggc aaagcggcgc acaagatcaa ggaactgact aacgacactt ttgcgcttcc
    47401 gaagcgctcg atcaaacttc ccaccagaga tagagcggtg atcatgccca gcgctgcgcc
    47461 gaacaggatc aaccccaatc tcccttcgct cagcgcgagc ttgttctgaa tgaccgggat
    47521 gcgtgccacc caggtagccc caaccgcacc gttcgtaaaa aaagctgcca gcactgccaa
    47581 ttgggttttt cctccaacca ggaatttttt catgaaaagc tcctttgtaa ttgttgaact
    47641 cggttctgcc tgcacctgga tcattcagct ttgaagacct gagttcccag attgcggtac
    47701 ggaacaagaa tttcctcttt cacctgtttt tcagtgatga tgtgcgtgat ggctgtgcat
    47761 ggtgctacaa cgtatggacc aaccgtcccc agtttctcct cagtcaccgt ggcaaccact
    47821 tcgccggatt gcgcgatgaa ggcgcgcatc atgttcactt cttcatagtc atagatgctg
    47881 catcccactt cggggtgtaa tccgcagatc cccaaaaaac agatatctgc ctgaaagcgc
    47941 cttatttgtt caatggcttc ggtatcgctg gtgaccagtt cccttttata caaatgcccg
    48001 ccaatgagaa tgactgcgat gttgcggtag ttcgccaggt gcagagcgat ttgcgggctg
    48061 atggtgacga tggtggcgcg cagatcaggg ggaaggttct tggcgaggct ggtgttggtg
    48121 gttccgcctc ccagcaaaat cagttgccca tcccgcacca gttcgcgcgc agcctttgcc
    48181 agtttgatct tgccaaacgg attttgttta tcccgttctg taaatgggat gttgtttggc
    48241 gcgatgggga gtgccccgcc gtgcacccgc ttcaataacc cggcggctgc caggtcccgc
    48301 aggtcgcgcc ggatggtatc ttcagaaacg ccatactgac tgctcagttc agatgccacc
    48361 acacgaccgc gcagatgcag ttcatccaga atagcctgct gtctttcctg gggtaagtct
    48421 atggtttgca tgaggtttcc tttgaacgcg catggttatg cgtgtaaatg cgtgtttatg
    48481 cattatattg catgtatttt aaaatgtcaa ctgacccccg tcgcttccgt cccgtttgct
    48541 tgcacgattc gaatcgcgaa aatgctgatt ggaactggcg gttaccgcta agcctctatg
    48601 cagatggggt tttgttcgtg ttttctcaac tctccgctat aatttttata tggaattcca
    48661 tatttcctcc aaagcgcgga agaagtacca gttcgaagaa tccctcttct cctttgatgg
    48721 caatatcatc tttgccaatt tccacgcggc gcgtttgttt gcccagcgca tgaatgaaaa
    48781 acgcgatctg accgcccatc ctgatctcac agtgcgtgcc gggcaggtga atgctgtggg
    48841 gttgatcgat gagattctcc attatgtggt ttccatatat cgcacgcaaa aagctccccg
    48901 gctctatcaa gatctggaag ctgctttgcg gaaagggttg ggaaagcgca aattgactgc
    48961 cgttctgcgg gcattcacgc gagattttcc gcccgccgca gtttttcagg aaaaaatgat
    49021 gccggatgaa tatctttccg gtgagaccaa tggcattccg aaccaccatt ccacgcttga
    49081 agagatcctg atattgtgga tcacgaatca aaatccggct tgtttgccct accaggagtt
    49141 gtttgatgac cgcgatctgg gtgcgaaaac ctcgtattct gatctgatca ccaccctgca
    49201 cgcttttttt gaaacccaag ccacctttgg accggataac caaaacctgg tagatatgct
    49261 gcgcagtccg gctattgctg tacctgattc actgcgcggg cagttggatt acatgcgcag
    49321 ccgctggggt gacctgctgg ggcactattt gctcaaactg cttggcagct tgaacctctt
    49381 cgctgaagag gaacagatgc gcggaatggg tcccggtccg gtgcgcatcc cggtgtatgc
    49441 cgcaccggac ggattggaag ccgagcgttt cagccccgat gccgattgga tgccgcgcct
    49501 ggtgatgatg gcaaagaaca cctatgtctg gctcgatcaa ttgagcaaac ggtaccatcg
    49561 cccgatcata cgcctggatc agatacccga cgaagagttg gagcgcctgg cggattgcgg
    49621 ttttaccgga ttatggttga tcgggttgtg ggagcgcagc cgggcttcgg cgcgcatcaa
    49681 acaggcgtgt ggtaatcccg aagccatcgc ttctgcttat tctttaaagg aataccaaat
    49741 tgccggtgac ctggggggag aagaagcgct catcaatctg cgcacccgtt gtcagcagcg
    49801 cggtattcgc ctggcgagcg atatggtgcc caatcacatg gctatcgatt ctacctgggt
    49861 cgtggaacat cccgattggt tcgtttcgat gccctattct ccttttccgt cctatacctt
    49921 tcgtggcagg aacctgagtg aggatgagcg cggagagatc tacatcgaag accattatta
    49981 tgaccgtacc gatgcggcgg tggtgttcaa gtatcatgac cggcgcagag atcgtaccta
    50041 ctacatctat cacggcaatg atggcaccag catgccctgg aatgacactg cccagcttaa
    50101 ttacctcgat cccaatgtgc gcgaagcggt catccgcact atcctggatg ttgcccgccg
    50161 ctttcccatc attcgttttg atgccgccat gacgcttgcc aagcggcatt accaacgttt
    50221 atggttccca tcaccaggtg ggggatgcga catcccttcg cgcagcgatt tcagcctgcc
    50281 gcaagatatt tttaaccagt acatgcccaa cgagttctgg cgcgaggtgg tggatcgcgt
    50341 ggctgccgag gctcccgata ctttgttgtt ggcggaagct ttctggttga tggaagggta
    50401 ttttg
//
LOCUS       DAOSDS010000005        45479 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_468_length_45479_cov_5.964336, whole genome shotgun sequence.
ACCESSION   DAOSDS010000005 DAOSDS010000000
VERSION     DAOSDS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 45479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45479
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_468_length_45479_cov_5.964336"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="PLL88_01295"
     CDS             <1..411
                     /locus_tag="PLL88_01295"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ00227.1"
                     /translation="FLSAICWAFYSNLIRKHNLDGSFVGYFMIVAGLIMGLLSLFVTE
                     PSNWSFEVVFQIVYRSVLTIFVATHLWNRSITEGDIYKVSIAANYSPLIGVVVTSIML
                     GVKLQPVVLVGAALLILGNIFNIRINKEKVIPAS"
     gene            complement(706..1503)
                     /locus_tag="PLL88_01300"
     CDS             complement(706..1503)
                     /locus_tag="PLL88_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ00228.1"
                     /translation="MLPQYHIKLVEGDVAPYVLLPGDPKRVEVVASFWDEAHKVADNR
                     EYVTYTGVYKGAPISCTSTGIGCPSTAIAMEELARVGAKTFIRIGTCGAYQDSINIGD
                     IGIFDSAARYDGASHLYAPPQFPAVATHEVVSACIQAGKDLGYRYHVGTTRSADTFYA
                     KHSRPGSSFNDFWQSDWQYHFEDLKRLKIIAGEMEASVIFVLARVWGLRAGGISVVLD
                     NVLKVTGDDGNFDPEKDIAHGKEYIDKLSIMGSEAVRILFEEDQKKS"
     gene            complement(1512..2318)
                     /locus_tag="PLL88_01305"
     CDS             complement(1512..2318)
                     /locus_tag="PLL88_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ00229.1"
                     /translation="MLPQYHIKLVEGDVAPYVLLPGDPKRVEVVASFWDEAHKVADNR
                     EYVTYTGVYKGAPISCTSTGIGCPSTAIAMEELARVGAKTFIRIGTCGAYQDSINIGD
                     IGIFDSAARYDGASHLYAPPQFPAVATHEVVSACIQASKNLGYPYHVGTTKSADTFYA
                     KKPVGYSFNDFWQSDWEHFADDLRRLNIYFGEMEASVIFVLARVWGLRAGGISVVLQS
                     AFKSEEKTKDLGKYDPEKFIDHGKEHIEKLSLAGSETVRVLYEEDQKKDQ"
     gene            complement(2369..3322)
                     /locus_tag="PLL88_01310"
     CDS             complement(2369..3322)
                     /locus_tag="PLL88_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ00230.1"
                     /translation="MLKERNRPSVVGIGDLVVDVVTSIQDFPINADCFYPASQIYQSP
                     GGRCNFLIELSRLKVRAVAIDVIGKDAWGNFLCKTLENENIDLSLVQKDQDTSRSIVL
                     SDIHGNHSFIGKFGHNSPYQYGNTYYSTISKASAVFASGYNFQNRNSENLTLNTFKLA
                     KKLGIIRGFDTGPVFHLLDEDKKMEVYKLCTHLFLTEDELELLGNKNKVSAFFDHGIE
                     VVIIKKGAKGCQVITNHEETFSIPGLKVPVTDTTAAGDCFDAGFIAGLMKKFPLESCA
                     LLANCVGAAKVKKLGGGVNVPTFEEVFGIAKEFNVRLDQDK"
     gene            complement(3315..4352)
                     /locus_tag="PLL88_01315"
     CDS             complement(3315..4352)
                     /locus_tag="PLL88_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ00231.1"
                     /translation="MSLKEYSKLDPKYLFEHVYGGLIGQALGDAFAMPAYFKPKDTWD
                     FYGGWINDFLPGPSDHPVHAGLAPGEVTDDTQQAMSIIKEILINKKISVELAAKAIIG
                     WYDLIDGDHNPHVGPSTRKACLQLKGGVDPHETGVLGDTNGGAMRISPIGLINPGNIQ
                     QAILDSIIVCTPTHFTSVAISGACAVAAAIATALVPDSSIESIVHSAKIGADEGRKHG
                     NPWLGASISKRIELAVQIVEKESDVYERILALYDIVGSTLATSESVPSALGIFVLAKG
                     DPMTCARYAAALSGDADTVGAMACAIAGSWKGISSIPTDIRNTIEKVNKDYDFFGTAK
                     EIVKFIEERNA"
     gene            4524..5669
                     /locus_tag="PLL88_01320"
     CDS             4524..5669
                     /locus_tag="PLL88_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ00232.1"
                     /translation="MRKFISFVMILVVLFVSTSCVSSKAPETEVAEIEAPEEVPAQGA
                     VIAECISATASIDDRSFCQGAWEGIKAFGDENGLPYAYYQATGDSTTDLVNCIGVAVD
                     GGAEIVVVPGFAWGAPVFLAQDLYPDTKLVILDGDPHSEDYSEYRQEDNVYSIYYAEQ
                     ESGFLAGYAAVKEGFTNLGFIGGMAVPPVVRFGYGFVAGAEYAAQEMGLEEGSITMYY
                     HYLGNFDVSPENQTYAASWFSSGIDIIFAAAGDVTSVVSSAASQTGEGKWVIGVDVNQ
                     GWDADNVLTSATKNLRGSTYQALDLYFNGEFPGGQVATLNSSNEGVNIPTDEETWRFS
                     TFTMEEYEAILSKLSADEGGIASSIPVDTGYATADLIPLRYVVVTVY"
     gene            6076..7611
                     /locus_tag="PLL88_01325"
     CDS             6076..7611
                     /locus_tag="PLL88_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00233.1"
                     /translation="MEYAVELLNIKKQFPGVLANDHITLKIKKGETHALLGENGAGKS
                     TLMSILFGLYSSDEGTIKINGKEVKITNPNDANALGIGMVHQHFKLVHNFTVLENIIL
                     GVESTKNKLLDMASARAKVMDLSKRYGLDIDPDKIISEITVGMQQRVEILKMLYRAND
                     ILIFDEPTSVLLPQEVDELIKIIKGLAAEGKTILFISHKLEEIKRVADVCTVLRRGKV
                     IGTVDVATTTKEQMAEMMVGRKVLLQVDKGDGYKGEKRPVLEVKDLCVEDKESGKLRV
                     DHATFTIHAGEIVSLAGVDNNGQKQLVEAITGIIPSNGEIYLCGENVTERNIRYRNTH
                     GLSHIPEDRQRHGLVMEFTVSENSILQEYFNDEFQKSGFIKRAVVDQYADELIEEYDI
                     RAGRGGETTAGSLSGGNQQKLILARELERDHELVIAVQPTRGLDVGATEFVQKQLITQ
                     RNAGKAVFLVSLELEEVMSLSDRILVIHDGKIVVSDLLPSKIDMMELGMYMAGSKGVD
                     KNA"
     gene            7604..8695
                     /locus_tag="PLL88_01330"
     CDS             7604..8695
                     /locus_tag="PLL88_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00234.1"
                     /translation="MRKTKKTWVDNFPDLTASLVACVIGLLVGFLVLVVTNPENSIKG
                     FKALLTGAVSGGFTDFGNVIVYAGIYILCGLSVGFAGKTGVFNIGLSGQFIIGGVAAL
                     YVGIMWTWLPPGIHCLVAVIAGLLAGLGWSSTIGFLRAFFNISEIVVGILLNYIGVYL
                     ANMIILDKFYDSYANRSQAVQSSALIPTLGMEKIFPGTGADITFFVAILLAIVIHVIL
                     NKTTFGYEIRTCGFNKDAGIYSGINAKKNVILSMAIAGALPGLAGAMFYLSSSHNYIQ
                     IVDSAPAIPMRGIVVSLVAMGSPIGIILSAIFIAYITVSGLDMQLYGYVPEIVDVVTS
                     VIIYCTAFVILIKKAISTILRKSHDLNEG"
     gene            8701..9654
                     /locus_tag="PLL88_01335"
     CDS             8701..9654
                     /locus_tag="PLL88_01335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00235.1"
                     /translation="MDIVYFIVQQLMFFMVPLLVVALAGMFSERSGVFNIALDGTMIV
                     GAFCGTLFLNLTQDSWSGQYQLLVALLIAGISGILFMLLHAFASVTLNASQIISGIAL
                     TFIAPALAIYLARVIFGVQRIFFTNAFLIRKIPILGDIPVIGKLFFTNTYLHIWIGYL
                     ILLVSYVVLYKTKFGLRIRACGENPYAADSVGIDVKKMRYYGVLISGFLAGMGGLIFI
                     VPTTIQFSGSVASYGFLAIAVLVFGQWKPFKILIASFLFALAKTIASSYTSIPALVAL
                     GWSPYIYKMLPYVFTIVMLVLTSKNPQAPKAAGKPYISGGE"
     gene            9843..10937
                     /locus_tag="PLL88_01340"
     CDS             9843..10937
                     /locus_tag="PLL88_01340"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOJ00236.1"
                     /translation="MKSLRMYITGPQKYYPNGLKELKMMKAYAEMYGFEVINDFSYIE
                     NGFRDYEHIDRNFLDDCDVVVADVNPFRGGEPESGTVFDMGVAYSKSKKIYSHVKDLR
                     NVVHKYPYAYFDEEGNIIDEMGLIFRAGQTPGNLMYMVPSKMVEGGFIECVKALVTDL
                     NEEAKDKESRVKPRIDYRNQATWSVKEGEYRAYLAGFECFSTNTKEVGDGMKKICEKY
                     NFQGIFPPDIPPDFGELSEEGFKNVYTRCAYFFDRDQLHIRNSNMVIANLNPYHGHEP
                     DSGTVFEAAMSYGLGYPCYCFISDERPLIERISCRKDENSIYRDIEGFVVENFGFPLS
                     SRLATTMKVIVADFDETARLVAKDLGIFTD"
     gene            11122..12339
                     /locus_tag="PLL88_01345"
     CDS             11122..12339
                     /locus_tag="PLL88_01345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00237.1"
                     /translation="MNSLVKTISQQNQNVKISFVFKSMYGLSQGIFLVVYNLYLIELG
                     IPLDTIGMILSANPLALAVGSIPVGYLTEIIGFKKSFLLIYGISGLSLLIQIFISKVE
                     WILLAAFVCGLALSGDFVANLTFLAHNTTENDRAKIYSYSSLFFNLFLSIGSLIAVVL
                     PNILINLGMSLLIVYRLTLLMSGGLLIAAIIPGMKISQDLYSQERKVSFQPYFQGINT
                     FTVKQILTLLCLGISLGVLTPFMNLYFIYHLGSNREFFGTISALNVFFISFAIIVGPL
                     IVSKWRSVPAVTVVRLLFVLFLCVFTYTHSPMLGASAYWVMMSLFQMSQPISLAFAIK
                     ASTKKTMTAVTSWINVAFWLGYALAAPVVGFFMSKSRYQHPLMIAIMTMILAASFNHL
                     FFNKIEKAIENTK"
     gene            12460..13392
                     /locus_tag="PLL88_01350"
     CDS             12460..13392
                     /locus_tag="PLL88_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOJ00238.1"
                     /translation="MTNKIIIDTDPGIDDALAICMAIHSPEISILGITSVFGNTYGKI
                     TAQNALRLSELSEHQVIPVARGSDIPLVMPIQNLGTIVHGEDGMGDSDLPSPRKELSS
                     QSAVELIAKIIRENPGEITLLTLGPLTNIALALRVYPELIGQIKKLVIMGGVVSRPGN
                     MTPVSEANMGRDPHAAEIVFAAELPTVLVGLDVTEKTIITNSLLDEIFAVKNPTTRLL
                     KKIIPAYQHFFKEYYCMDGSFYAHDPSAMAYVLKPDLFQTRKAPIFVETEGRCLGQTI
                     ADWDHKWDKRTEVEVCLEVDSQGVLDTIKASLIS"
     gene            complement(13584..14996)
                     /locus_tag="PLL88_01355"
     CDS             complement(13584..14996)
                     /locus_tag="PLL88_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00239.1"
                     /translation="MKKSALLLCTISILLLCACTGGKEAESTPTATATSTAISTATKI
                     IPSATPTTIPSATPTTIPTETAVPTITPTPLPYIDNPISGINVGPYLYDDPNMGATVS
                     EADLRALIERIAPYTYWIRTYGTENGLENAGAIAREFGLNIAVGAWLSNDLTTNESQM
                     TSLIEMAENGDVDLAIIGNETLLRGDLTERELMIYINWFKEAVPDVPVTTSDVWSELK
                     QYPDLIEAMDVVAVNVYPYWDNVTIDKATSTIEDWYLGATNTVNAISPDKQIIIAETG
                     WPSCGKNGDPANAAAYFNSFTSFAQMLDVQFFWFEAYDEAWKVSNEGEAGACWGLWDG
                     EGNLKPGMEETFSGMFAESFADPNLVVTYIPPQGSLDDLEGIALHVNPDDYRVVVYIY
                     VPNANGWWLKPAFDFPYTEIDESGYWSCPIFTGGIDDQATKVAAFLIPSNYEPPLASG
                     WSSLPQALYDASVSETEVNR"
     gene            15086..15367
                     /locus_tag="PLL88_01360"
     CDS             15086..15367
                     /locus_tag="PLL88_01360"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00240.1"
                     /translation="MRYIRVGDRGVVDSLRVECDGVLILVDIFGALPCNVAAMTMAKY
                     PDVDVVTGLNLPMLLEVLLQRNTHSLLELTKVAKRYGVEGIKTISDLIS"
     gene            15578..16366
                     /locus_tag="PLL88_01365"
     CDS             15578..16366
                     /locus_tag="PLL88_01365"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOJ00241.1"
                     /translation="MDILVCIKQVPGTSAVKIDEKTGSLMREGIDSKMNPYDLYALET
                     AFQIDQRTDAHITVLSMGPSPVREIIQEAFLMGDADGVILTDRRLAGADCLATAYAIS
                     QCIEKIGLPDLIICGKQTTDGDTAQVGAEIAEFLHIPHITNVLKIEEIKTESLIVEAD
                     LPAMTQRAEIRFPALITVEKGIYQPRLPSFKRKQRINDQKIQVYSVQDLADQNEAHYG
                     LSGSPTQVLRIFPPETEITSELWKDSAQDLAQKFYDILVERKFI"
     gene            16376..17608
                     /locus_tag="PLL88_01370"
     CDS             16376..17608
                     /locus_tag="PLL88_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HOJ00242.1"
                     /translation="MANIVIHQEKIQNAADLINICPFGAIQLEDGVLVITSACKMCKL
                     CIKNGPVGAFELVEQPLIEIDKSAWRGIAVYVEHTEGNIHPVTFELIGKARELAAKIA
                     HPVYAVFVGNHIEEKAQELRYFGVDRVYVYDRAELQDFRAEPYAAAMEDFISTVKPCT
                     VLVGGTTVGRSLAPRVAARFRTGLTADCTKLDMQPNTDLDQIRPAFGGNIMAHIRTKN
                     HRPQFATVRYKIFSAPQRLAQPFGEIQSMDVAPEKLKSGVCVLSIQPKQTVENIEDAD
                     VIVAAGRGIKKEQDLQMIRILADRLGGQVASTRPLIEAGWVDPRRQIGLSGRTVSPKL
                     LITCGISGSVQFTAGMSGADFVVAINQDPDAQIFKVAHIGLVGDAYQIIPALLEKIDF
                     RRTGSVLDAIKTIQSEVA"
     gene            17608..19005
                     /locus_tag="PLL88_01375"
     CDS             17608..19005
                     /locus_tag="PLL88_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOJ00243.1"
                     /translation="MEYKKIDAADVAALVAICGREHVKRREVLSEDFSHDELAGIRVY
                     PEVMVEPVSAEQVSAIMKHAYERCIPVTPRGQGTGLVGGAVPVREGILLNLCQMNKVL
                     ELDEATMMLTVEAGVLLMDIYKYVEPYGLFYPADPGEKSATIGGNINTNAGGMRAVKY
                     GVTRDYVKGLEVVLADGTVLMLSGKNAKNSSGYSLKDLVIGSEGTLAVITKAILRLIP
                     LPLWVVSLLVPFNDLNTAIRAVPHLAMLKAMPTSIEFMEREVILAAEEYLGKKFPDKS
                     APAYLLLTLDGNSKTELDAAYCAAAERCLECGALDVLIADTDERREMIWSARGAFLEA
                     IKASTTEMDECDVVVPRDRIAELVGFTRQLQREVNIRIMSFGHAGDGNLHIYALRDAL
                     SEEDWQRKLDDIFEKLYARAKELGGQVSGEHGIGFAKKNFLADSLGEKTIDLMCGIKQ
                     VFDPRNILNPGKICQ"
     gene            19501..20793
                     /locus_tag="PLL88_01380"
     CDS             19501..20793
                     /locus_tag="PLL88_01380"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF023401.2"
                     /note="catalyzes hydrolysis of cellobiose 6'-phosphate
                     into glucose 6-phosphate and glucose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HOJ00244.1"
                     /translation="MKIAVIGGGSTYTPELVKGFLDRADSLPLDELWLMDIDAERLAI
                     VGEFTKRMVKARGEPFRVVLSQNQQEAIADSAYVISQLRVGKMRARREDEYLGQRHGL
                     IGQETTGVGGMAKALRTIPVVLKIADDIRQSAPGALFLNFANPAGLVTEALMRYAPAI
                     QAAGLCNVGITTKMEILEKLEKATGFSIEADRAALNTLGLNHLSWHRGFTVDGEELWS
                     KIFPAYVDSLRKEAEPEWDVRTIESLSMIPNYYLQYYYYTHKKLEAQKEWPPSRAEQV
                     MEIEKRLLNDYENPALTEPPASLMERGGAYYSTLATQVINSHYNDLGQIHVINTRNDS
                     AINAWPSDWVLELPAKVDKLGVHPLSAEPLPAVCFGLISQVKMYELLTVEAAVHGDRN
                     AAYQALLAHPLGPSADCVQQVLDDMLDTNRRWLPQFFE"
     gene            20839..21729
                     /locus_tag="PLL88_01385"
     CDS             20839..21729
                     /locus_tag="PLL88_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ00245.1"
                     /translation="MTANPLLKKLGYSDTDRLVIIHADDIGMCQASIQAFKDLWEFGT
                     LSSGAVMAPCPWFLAAAEIARQNPTMDMGVHATLNAEWATYRWGPLSTRDSHSGLMDA
                     EGYLHRSVQAVVESATVEAVTVEVEMQVERVLAAGIDATHIDSHMGAIMHPKFIQAYL
                     QAGMTHQLPNMLPRASAHGFDTMGIDESTLASFTPFLNELEAKKVPMLDGMFSMPLEH
                     DHDHIHVAKQLLRNVPIGISHFILHPSVDTPELRAICPDWSARVANYHAFMSHELKQF
                     LKDEGIHLIGYRLIRDGMRS"
     gene            21771..23147
                     /locus_tag="PLL88_01390"
     CDS             21771..23147
                     /locus_tag="PLL88_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00246.1"
                     /translation="MTTTTVKDFATSTKTRMMYALMSLGVAVPIETIGAFILYFIVDI
                     KNLPATWYATFWFFYTIYNALNNPALGYASDRTRSRWGRRRPFILFAGVPYALTFALI
                     FCTPFDGKDNPIALLIYFGALIVIWEGLYTAMATGYYGLLPEMFGTYQERTDVAAKMN
                     IFQVIALVLASVVPPLLWNKIGWGGMAISLAIIAIIPIYIGFPFLFERKDLATDTSFP
                     FLKALKLTFMNKSYLTAAGAQAMRFVATGILQMGMPFYLKYSLKVSDSMFSIFFGVAF
                     LVSMLSLYPWRNWVANKLDSRRTLMLANLVMIIGVIPLGFSPSFSFVLASAFIIGVGL
                     GGLILMGDVIMGEVVDEDEVKTGQQRAGMYFGMSGFIITLSGLLVSSIFGILLPAFGY
                     DTLLEVQPASVDLGFRIFLTVPSAIGLLLAIVFLKLYPLHGKRLQDIRDALAKKKTLA
                     EKQGESSV"
     gene            23322..24308
                     /locus_tag="PLL88_01395"
     CDS             23322..24308
                     /locus_tag="PLL88_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOJ00247.1"
                     /translation="MKYFLGIDVGCSKTHALIADEAGVCRGFGKSSGGNHQVVGYAGL
                     AAVLKESLEQACRMTNITADQIAGAGFGIAGYDFPSDHDPHWEAVAALGLSCPIEIVN
                     DGVNGLLAGTSHYVGVNVTAGSSVNCRGRAPDGRKGRITGNGISFGEFGGGGEIVWKG
                     LHMVNYAWIQRISPTALTQIYLETTGAKDEMDLMEGLSNQTYQLSPVIAVKIMEAALA
                     GDAAAGQILHWAGQELGWLAVSVIRQIGMQNEEVEVILSGSIFDGGELISDPMRQVVL
                     EYVPGAKLMRLDGPPVAGPLILGMQLAGHDPYPLRSKLIKTAKTLVEPKSAF"
     gene            complement(24617..25348)
                     /locus_tag="PLL88_01400"
     CDS             complement(24617..25348)
                     /locus_tag="PLL88_01400"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ00248.1"
                     /translation="MFIHDYYDNTAEILKKIYLEETDAITQTGKILADVFKRDGLLHV
                     FGCGHSHMIEEELFFRAGGLAQVNPIFETSTMLHEGAVKSSQIERLSGYAHLVLERYP
                     VTPNDAMLIVSTSGINSFPIEMALAAKDVGTTVIGITSLLYRDYPSRQKDGLHLSDVC
                     DFTINNHVPVGDACVQVKSDGTKSGPLSTLANTYISNSLILAACDQLKAWGIEPKVYR
                     SGNCQGGDEYNAELINQLRPRIKNL"
     gene            complement(25356..26099)
                     /locus_tag="PLL88_01405"
     CDS             complement(25356..26099)
                     /locus_tag="PLL88_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ00249.1"
                     /translation="MATEKTEKKNKSTHVKESLRELINQPGSKHGDIIPSERKLGEQF
                     NVSRITIRKALEELESEGLLYRVRGKGSFICVEKIPQKLALLTSYSEDMLERKLTPDS
                     LILNIDSIIANSCISNKLQVQINDPVYVLRRLRLADGEPMAIENCYMRQEIGKIIAET
                     IINGASLYKLFATKCDLTLYNAYQTIEVARLTNWERQLLGKDSPTAALLTKRQTFDTN
                     NQVVEYVESKYRSDRYLFHVELFAYQLSK"
     gene            26325..26753
                     /locus_tag="PLL88_01410"
     CDS             26325..26753
                     /locus_tag="PLL88_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ00250.1"
                     /translation="MIKKENIQAKVKAKDWSDAIQIAGRLLEISESATHRYTKAMITA
                     VKDLGPYMVITPNFAFVHAAPSEEVKKNDVALITLDKPVLFGSRNDPVYIIMAICSKD
                     GKSHMNSLAQIANILMQENTIAKLIEAKNVEEIFNIFEKK"
     gene            26822..27109
                     /locus_tag="PLL88_01415"
     CDS             26822..27109
                     /locus_tag="PLL88_01415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ00251.1"
                     /translation="MEDIKIQTVCGFGCGSSLLLRMNIEEVLKENNLKATVFCGDLGT
                     CLSQPCDAIFISEELWSRIEGRTTVTIVPIKNFMDKKEITTKTMEFMNSLN"
     gene            27134..28405
                     /locus_tag="PLL88_01420"
     CDS             27134..28405
                     /locus_tag="PLL88_01420"
                     /inference="COORDINATES: protein motif:HMM:NF006920.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HOJ00252.1"
                     /translation="MEILNFVINEIFGQGAIFLALIALIGLLLQKKSFSEIVRGTVMT
                     AIGFFILTQGTGIITGKSIDGLATAFNTIMPSASGQPDIDMSQFGTEIGIVMIIAFAA
                     NLLFARFTKWKSVFLTGHMLYWFPYVFIAAGVDAGLSGLKLVVLAAVVTSIYMVVAPN
                     LMRPLVKEVTGDDSFTIGHPTTTLSVISGYLGKLIGNKEKSTESLKIPKSLDFLREVS
                     ITGSLVICLTYIVMSFILKANGFVPGEVWGYAGGSTGAFTYIFTKSIYFGVGVTVMLL
                     GVRMLIAEIVPAFKGIAEKVVPGAIPALDCPVIFNYAPNALIIGFVVAMITSTITIIL
                     TAGTFPTVIIPLTFTCFFEIGTAAIIGNGTGGTRGAVVGAAVSGIIMVFLVGFGSYFF
                     SNTINNWMLVYGGQDFSLWGIITGLIAKLLV"
     gene            complement(28636..29532)
                     /locus_tag="PLL88_01425"
     CDS             complement(28636..29532)
                     /locus_tag="PLL88_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HOJ00253.1"
                     /translation="MLLFENSIMRAKMVDVRHLLDLRNTIHSQYRQNLFENEFYEERV
                     KDFEDLSSNVMPNARSLIVVAYKDPPVHFSFTHGGKKIELLVPPTYLFAQRKLRKIMD
                     VMTGALESKGHHIVQAFLPKKLLAVCSGLAEYGKNNITYVKGFGSYHRLVSFSSDLGC
                     EQDEWREPVMMERCKSCKACTLNCPTGAINPERFLLHAERCLTYLDEKPKKVPFPAWV
                     KKEWHNCLVGCMHCQSICPENKGMRGWIEEGEDFCEEETALLMSGKKAKELPKPLREK
                     LTRCDLLDMLDLFPRNLSVFFE"
     gene            30122..30670
                     /locus_tag="PLL88_01430"
     CDS             30122..30670
                     /locus_tag="PLL88_01430"
                     /inference="COORDINATES: protein motif:HMM:NF010182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HOJ00254.1"
                     /translation="MFKINTKTIVAIGLGAAIFMLLFMYVKVPSPVPETSFQTAYGLS
                     SFLATLFGPIAGALISFIGHALSDAVQYGSPWWSWVIASGVSGFIFGFAFNRTKVEEG
                     EFKGKDILTFNLIQIIGNVVAWVVVAPVLDILIYQEPVNLVFTQGITAAIMNIVSAGV
                     IGTLLLVAYAATRTKKGSLKKK"
     gene            30698..30907
                     /locus_tag="PLL88_01435"
     CDS             30698..30907
                     /locus_tag="PLL88_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00255.1"
                     /translation="MSGRDDIEQSRSAVGGFFTPARGRINQQASFPMGEIVVILWHEQ
                     MPAPVSKAPSSGDRGVNRAPATFFC"
     gene            30920..32629
                     /locus_tag="PLL88_01440"
     CDS             30920..32629
                     /locus_tag="PLL88_01440"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00256.1"
                     /translation="MEKRAIIDFDHFIFQYFSQAEPTLYDINLKIYPGEKILIVGPSG
                     SGKSTLGHCINGLIPFSYKGEITGSLTINGKDTRELNVFELSKMVGTVLQDSDGQFVG
                     LTAGEDMAFALENDCVRQNVMHERVQKVAQMVGMESLLNSSPFELSGGQKQRTSLAGV
                     MIDDVDILLFDEPLANLDPATGKKAIEIIDDIHCGSDKTVIIIEHRLEDVLYRHVDRV
                     IVLDEGRIIADMDAHELVCSDLLRKTGIREPLYITALKYAGARVTCDMQPGYIATLNT
                     NSAGAAVRNWYKGIQPRQRSNVNPSIIQMEHISFSYDGVRPVLQDINFDIREGEMVAL
                     VGKNGAGKSTLSKLLCGFEKEDKGVIRYCGENIRGKTIKERAEIIGLVMQNPNQMISK
                     AMIYDEVALGLRLRNIPEEEIKPRVERVLTICGLAPFIEWPISALSYGQKKRVTIASI
                     LVLDPRILILDEPTAGQDYRHYTDIMEFLVEINKLGVTIILITHDMHLMLEYTPRAIV
                     LAGGRLIADTAASVVLTDAQVIAEANLKETSLFNLAHRCGIADGTAFVQGFIDYEREL
                     KNR"
     gene            32626..33465
                     /locus_tag="PLL88_01445"
     CDS             32626..33465
                     /locus_tag="PLL88_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOJ00257.1"
                     /translation="MNTKMKLFSYNTVDTFVHRLSGLTKLMCFLLLTFAVMYSYDLRV
                     VLLVMLFSFIVLKIARVQFSQIRTMVIYVLVFVITNAIITFFFAPEFGVEIYGTRHEI
                     ARVVGKVYLTQEQLLYQITKFFKYTSVIPLGMIFLLTTNPSEFASSLNGVGVPYKAAY
                     AVALTLRYFPDIQRNYHDISLAQQARGLDLSRKAKFGDRFKNALLIIIPLIFSTLDRI
                     ELISNAMDLRGFGKSNKRTWYTFRRLNRQDFAAIVVSLLIFAGSLCVSAFINHSRFYN
                     PFI"
     gene            33478..34320
                     /locus_tag="PLL88_01450"
     CDS             33478..34320
                     /locus_tag="PLL88_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HOJ00258.1"
                     /translation="MKPILVFQTDFTYKEGAVCAMYGVVKSVDRELEIIDGTHEIPRY
                     DIWSASYRLYQSMRFWPQGTMFVSVVDPGVGTKRRACVARTTDGYTVVTPDNGTLTHL
                     KQRVGITAVREIDERTNRLKGKGTEQTSVFHGRDLFAYCAARLASGIISYEQVGKEYP
                     VEKIVAQAILEAQVEKGRARGIIEINDPNFGNLWSNIPLELFRQAGFNYGERLQVTIR
                     YSGNVVYMNRVLFERSFGYAKRGEIILYTNELMRIGLAVNQGNFSEHFKISYGAEWEV
                     EITK"
     gene            complement(34637..35803)
                     /locus_tag="PLL88_01455"
     CDS             complement(34637..35803)
                     /locus_tag="PLL88_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOJ00259.1"
                     /translation="MQRSEFTKNASGEVIKTRQGYWAFVPKALPPRIHWTDTLMGISS
                     KADRSLARLAEVGNAFPAPHAVAQPFMRKEAVYSSQIEGTHTSLQQLFSYEAGQLSLF
                     RDMQNAHEVQNYVKAIDFGLERLKNLPLSMRLIREMHTVLMQGVRGESMAPGEVRQSQ
                     NWIGRAGATIETARYAPPPVEEMQICLADLEKYMHAKSELPPLLRIGMIHYQFEAIHP
                     FLDGNGRMGRLLVTLLLVAWGLLFQPLLSLSSFIEERRQEYYERLLAVSQRGEWEEWL
                     RFFLQGVHQQAEDTSRRILYLQTLRKQYHTMLAGESKRANLEKVVDYLISTPITSIKQ
                     AQESLQLGSFTTIERYFEKMVKLGILKETTGRARKRIYRSDAILRALEEEIVES"
     gene            36105..37091
                     /locus_tag="PLL88_01460"
     CDS             36105..37091
                     /locus_tag="PLL88_01460"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HOJ00260.1"
                     /translation="MNRAENKSARLTQIEALLMDRTDGLTRAELARRLGVHRSTIGRN
                     LVDMRAPTYEERGRIFIDRRAYLVNLRLTLHEALSIHLAGRFMATCMDRRNPHVASML
                     RKLGISLEKLAPLISHFVRSSANTFDDASKRQDLHYLQVLEEITLAWAEKRKVQLWYH
                     GVEGKVVKEYIFCPYFIEVGAVGQAVYVIGHIEPQSEMRTFKIERVERVEVLEETYSI
                     PDDFDPQNMLNQAWGIWFTEQEPVEVQLRFSARVAARVKETCWHPSERVSEQGDGSLL
                     WSAEIAEPREMLPWVRGWGADVEVLGPEELRVSIATEIKNLAEMYLMKITER"
     gene            37096..39573
                     /gene="cas3"
                     /locus_tag="PLL88_01465"
     CDS             37096..39573
                     /gene="cas3"
                     /locus_tag="PLL88_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOJ00261.1"
                     /translation="MEDFIAHVRQKDGKSQLLTDHLNETSVLSGQFAEKIGLKEAGEI
                     IGLMHDVGKASCEFQNYIRSASGQINPDADDYVDSQEMKGKIDHSTAGAQMIFRDLYN
                     KGAAGYFTAQVLSLCLASHHSGLIDCLAPDGSDVFSHRMEKSQNLTRIDEAMKNVKKI
                     GIELKFSSNFEKDLVAKFNSLQETNDSKETLTFKCGLLIRFLFSCLIDADRLNTADFE
                     SPNKVPLRNYGQYRPWETLIQRLNNRLVDFEKKENKNKVDELRNKVSQSCFNFSSKPR
                     GLYQLTVPTGGGKTLASLRFALNHVKIHNMERIFYIIPYTSIIDQNADEVRKILEDKD
                     KYGNYMDRVVLEHHSNLTPEEETRRQNLLSENWDAPIVFTTQVQFFEALFGFGTRGAR
                     RMHQLANAVIIFDEVQTIPIRCVHMFNLAIRFLVKSCESTVILCTATQPLLDKVDPIQ
                     RALSIRPEQQIITNTKELFEELKRVEVNDRRKIGGWTNEDVANLIEQELHDKGSVLVI
                     VNTKSSARALFQIINERKNKNAYHLSTNMCPAHRLEVLDCIKEKLSKKQPVICISTQL
                     IEAGVDIDFGSVIRYLAGLDSITQAAGRCNRNGERKLGNVWIVNPSKENIEKLKDIKI
                     GIEVAERVLDEFANDPDRFENDRLGLNAMEEYYKYYFYQRKEEMKYKVGKQSPVGRDD
                     DLFNLLSLNTISIAEYMRITNVAPAMPFRQSFQTASKAFNVIDSSTRGVVVPYGQEGE
                     EIVTELCGAFEIKKQYKLLKMAQRYSVSLFPNEFEMMTKKYAIQEVQEGSGIFYLDDQ
                     YYSHQFGWCDEIVNKMKSLIYQGGPYG"
     gene            39566..40279
                     /gene="cas5c"
                     /locus_tag="PLL88_01470"
     CDS             39566..40279
                     /gene="cas5c"
                     /locus_tag="PLL88_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOJ00262.1"
                     /translation="MDNIVEFKVTGKYALFTDPLTKIGGEKCSYQIPTYEALKGILGS
                     AYWKPTIVWIIDKVRVMNAIRTQTQSARPVNYGGGNSLAIYTYLSNVEYQVQAHFEWN
                     LYREDMAKDRNENKHYFIAKRMIERGGRRDVFLGTRECQGYVEPCKFGEGEGFYDNYG
                     ELSFDLMFHGFDYPDEIGKNEFYSRFWRPQMINGIISFIRPEECAIRKFVRLMQAEPP
                     ISIGLEEQGLLEGYEERRS"
     gene            40281..42191
                     /gene="cas8c"
                     /locus_tag="PLL88_01475"
     CDS             40281..42191
                     /gene="cas8c"
                     /locus_tag="PLL88_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOJ00263.1"
                     /translation="MSWIQKLYETYNNSQSMIGEESNDNEVPLLPLCHTTQKAQIEIV
                     INAEGDFQRARVILKNEARTIIPCTESSGGRTSGEAPHPLCDKLQYLALDYSSRTGNE
                     KQYTASYITQLEDWCNSKFSDSKIAAVLKYIKKGSIIEDLINYQILIAAEDGKLLSKW
                     EKKKKEDTPKIFEVLSSQDDAFVRWVVEIPGDSETKVWRNKTLWDRWMRYYASTKKEK
                     SLCYVTGEKDFTAEQHPAKLRNDGDKAKLISSNDSSGFTFRGRFLTANQAAAVGFETT
                     QKAHFALRWLISRQGYRKGDQAIVAWATSGVPIPKPTDDPLSILGNDELLTDELSTVS
                     TAQDVAIKLKRKIAGYGEEIGDTTRIVVMGLDSATPGRLAITYYRELTGSDFLQRINK
                     WHESCAWTHNYGSIEVRDEKSGKTKNKHVPFIGAPAPSDIAEATYGVKVADKLKNITI
                     LRILPCIIDGTQLPRDIVESSVRRASNRISMEKWEWEKTLSIACAVYKKYKRKENYQM
                     ALEENRKTRDYLYGRLLALAESLEQWALNLSHEDRETNAARLMQRFAEHPYSTWRTIE
                     LSLAPYKARLGGKSIKRQRMIDEVIASFNPEDFISDKRLSGEFLLGYHCQREILHPNS
                     EQSDNPIENEDE"
     gene            42206..43123
                     /gene="cas7c"
                     /locus_tag="PLL88_01480"
     CDS             42206..43123
                     /gene="cas7c"
                     /locus_tag="PLL88_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOJ00264.1"
                     /translation="METLKNKIDFAVIVNVKNANPNGDPLNGNRPRVNYDGYGELSDV
                     CIKRKIRNRLMETGYSIFVQSDDNRMDDYRNLRSRAEGVLKNVDMKNDKKYRDAACKQ
                     WIDVRTFGQVFAFSGKKGKAGEKDSENGEGTGVSIGIRGPVSIHSAFSVAPVLDRISS
                     IQITKSVSGEGDGTKRASDTMGMKHRVDHGAYVFYGSMSPQLATKTGFSDEDAEAIKK
                     TLITLFRNDASSARPEGSMEIYKVYWWKHNSKNGQYSSAKVHRSVEKRIRVKDGIDEP
                     RSIDDYEIPGKEEIEKQLPGLECEVLDGE"
     gene            43182..43853
                     /gene="cas4"
                     /locus_tag="PLL88_01485"
     CDS             43182..43853
                     /gene="cas4"
                     /locus_tag="PLL88_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOJ00265.1"
                     /translation="MKKPEYTADELLPLSGIQHFYFCRRQWALIHVERQWQENRLTAE
                     GRAMHKRADDPFFSEVREGKIIARSMPVASYTLGLSGVCDVVEFIPSPQGVQLPHRLE
                     KYLPVPVEYKRGKEKKDACDEVQLCAQAICLEEMLSTDIPQGYFFYGEVRHRIEVLFT
                     AELRDLVKRVSQEMHQYFERGYTPQVKPTKACQSCSLEDICLPTLQKKTISALKYIQT
                     YIDGE"
     gene            43854..44885
                     /gene="cas1c"
                     /locus_tag="PLL88_01490"
     CDS             43854..44885
                     /gene="cas1c"
                     /locus_tag="PLL88_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HOJ00266.1"
                     /translation="MKKLLNTLYITSPDAYLALDGETILIKKEEEVSTRLPLHNLENI
                     VCFGYQGVSPGLMAACAEHNIGLCFLSPRGYFLARVNGKIRGNVLLRKTQYRISEDEI
                     RSVEIATSFLMGKIFNCRKVLDRAVRDHSLLVDVDKLSDTSQALKECLNSVHGSKTIN
                     ELMGFEGSAAKLYFGVFDQLILQQKDAFQFVERSRRPPLDNTNALLSFLYTLLVHEVT
                     SALETVGLDPYVGFLHQDRPGRPSLALDLMEELRPVFADRLALSMINRKQVSSEGFIQ
                     KESGGILMDEETRKKVLSTWQERKQETITHPFLGEKIMFGLVPYVQALLLARYLRGDL
                     DAYPPFFWN"
     gene            44897..45187
                     /gene="cas2"
                     /locus_tag="PLL88_01495"
     CDS             44897..45187
                     /gene="cas2"
                     /locus_tag="PLL88_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOJ00267.1"
                     /translation="MMVLVTYDVSTIKESGKKRLHKVAKRCQNYGQRVQNSVFECILD
                     PGQLTQLKNQLIEIIDMEEDSLRFYYLGNEWKTRVEHIGAKKSIDLEGPIII"
BASE COUNT        12293 a         9614 c        11048 g        12524 t
ORIGIN      
        1 tttctgtcag ccatttgttg ggctttctat tcaaacttga ttagaaaaca caatttggat
       61 ggttcctttg ttggttactt tatgattgtc gccgggctga tcatgggatt gttgagtttg
      121 tttgtgaccg aaccttccaa ttggagtttt gaagtggtgt tccagatcgt ctatcgtagt
      181 gtgttgacaa tatttgtagc cacacacttg tggaaccgat ctataacaga aggtgatatc
      241 tacaaggtat cgatcgctgc aaattattct ccactgatcg gagtcgtggt tacatctatc
      301 atgctgggag tgaaattaca acctgttgtg ctggttggcg cagcgctctt aatacttgga
      361 aacattttca atataagaat taacaaggaa aaagtcatcc ctgcaagcta aggattaaaa
      421 aatagcgata gaggtcggca caatcgtgcc aatctctatc tgtgctttat taagatcgtt
      481 tgttgcggat aaaaatgaca catatggcat gttcctggtt gccttcaagg gcagcggaat
      541 gtcgtttatt gatttcattc atttttgcaa ataaaagtga acgaaaaatt taccctgtat
      601 tgctatcaaa cgccacgatc aaaccaggta gaatctcctt ttttaaatcc aaaaaaattt
      661 gaattcttta caaaaaattt ttcacattaa tctggcttca tcatattatg attttttttg
      721 gtcttcttca aatagaatgc gcaccgcttc gcttcccatg atggataatt tatctatgta
      781 ctcttttcca tgagcaatat ctttttccgg atcaaaattt ccatcatcgc cggtgacctt
      841 caatacattg tccagcacaa ccgatatgcc tcccgcgcgc aacccccaca cgcgagcgag
      901 cacaaagatc acactggctt ccatttcgcc agcaataatt ttcagccttt tgagatcttc
      961 aaagtgatat tgccaatctg attgccagaa atcgttgaat gaagaaccag gtcttgaatg
     1021 tttcgcatag aacgtatctg ctgagcgagt tgtgccaaca tggtagcggt atccgaggtc
     1081 tttacctgcc tggatgcagg cactcaccac ctcatgcgtg gctacagccg gaaactgggg
     1141 tggggcatag agatgagacg ccccatcgta acgggcggct gaatcgaaga ttccaatatc
     1201 accaatgtta atggaatcct gatatgcacc gcaagtgcca atgcggatga aagttttggc
     1261 acctacccgg gctaattctt ccatggcaat ggcggtagag gggcaaccta tgccggtgct
     1321 ggtgcaagaa atgggagcac ccttatacac gcccgtgtaa gtcacgtatt cgcgattgtc
     1381 ggcgaccttg tgagcctcat cccaaaaaga agcgaccact tccacccgtt tgggatcgcc
     1441 tggcagcagg acgtaaggag caacatcgcc ttcgactaac tttatatggt attggggtaa
     1501 catctttcct cctactgatc tttcttttga tcttcttcgt acaaaacgcg cacagtttca
     1561 ctacctgcca aggatagttt ttctatgtgt tctttgccat gatcaatgaa tttttccgga
     1621 tcatattttc caagatcttt tgttttttct tcggatttaa aggcgctctg aagaactacg
     1681 gatatgcccc ctgcgcgcaa tccccacacg cgagcgagca caaagatcac actggcttcc
     1741 atttcgccga agtaaatgtt cagacgtcgc aggtcatcag caaaatgttc ccaatctgat
     1801 tgccaaaaat cgttgaatga atagccaact ggcttttttg cataaaaagt atcggcagat
     1861 tttgtggttc ccacatgata gggataacca agattcttac ttgcctgaat gcaagcactc
     1921 accacctcat gcgtggctac agccggaaac tggggtgggg catagagatg agacgcccca
     1981 tcgtaacggg cggctgaatc gaagattcca atatcaccaa tgttaatgga atcctgatat
     2041 gcaccgcaag tgccaatgcg gatgaaagtt ttggcaccta cccgggctaa ttcttccatg
     2101 gcaatggcgg tagaggggca acctatgccg gtgctggtgc aagaaatggg agcaccctta
     2161 tacacgcccg tgtaagtcac gtattcgcga ttgtcggcga ccttgtgagc ctcatcccaa
     2221 aaagaagcga ccacttccac ccgtttggga tcgcctggca gcaggacgta aggagcaaca
     2281 tcgccttcga ctaactttat atggtattgg ggtaacatct ttcctccaat aaattaaaaa
     2341 taattgtgta ccctgtggtt aattgagatt atttatcctg atctagccta acattaaatt
     2401 cttttgcaat accgaagacc tcttcgaatg ttgggacgtt cacaccacct cctaactttt
     2461 ttactttagc tgctccaaca caatttgcta aaagcgcaca tgattccaga gggaattttt
     2521 tcattaagcc cgcgataaac cctgcgtcaa aacaatcgcc tgcggccgtt gtatccgtaa
     2581 caggcacttt taaaccagga attgaaaatg tttcctcgtg gtttgtgata acctgacaac
     2641 cttttgcacc cttcttgatg atgacaactt caattccatg atcgaaaaaa gcggaaacct
     2701 tatttttatt gcctaaaagc tctaattcat cttctgtgag gaacaaatgg gtgcatagtt
     2761 tataaacttc cattttttta tcttcatcaa gaagatggaa gacaggtccg gtgtcgaatc
     2821 cccttatgat tcccaatttc tttgcaagct tgaatgtgtt taaggtgaga ttttctgagt
     2881 ttctattctg aaaattgtat ccggaggcaa aaactgctga agcctttgat atcgtagaat
     2941 aatatgtatt cccgtattgg tacggtgagt tatgtccaaa tttccctatg aaagaatgat
     3001 ttccatgaat gtcactcaga acaatggatc tggatgtatc ttgatccttc tgcactaatg
     3061 aaagatcaat attctcattt tccagtgtct tgcaaagaaa gtttccccat gcatcttttc
     3121 caatgacatc tattgctact gcgcgaactt ttaatcgaga taattctata agaaaattac
     3181 atctccctcc ggggctctga tagatctgag aagctgggta gaaacagtcg gcattgatcg
     3241 gaaagtcctg aatactggtt acaacatcaa caacaaggtc cccgattcct acaacagatg
     3301 gtctattgcg ttctttaagc atttctttct tcaatgaatt taacaatttc ttttgcggtc
     3361 ccaaagaaat cgtaatcttt gttcactttc tcaatggtat ttctgatatc ggtagggatg
     3421 ctgcttattc ccttccagga accagcgatg gcgcaagcca tcgctcccac ggtatccgcg
     3481 tctccggata aagcggctgc ataacgcgca catgtcatgg gatcgccttt ggcaagcaca
     3541 aagatgccca aagctgaagg aacagattcg ctggtcgcca gtgtgcttcc aacgatatca
     3601 taaagggcta atattctttc atatacgtcg ctctcttttt cgacgatttg aactgccagt
     3661 tcaattcttt ttgagatgct cgctcctagc cagggatttc catgttttct gccctcatcg
     3721 gcaccaattt ttgcactgtg aactatggat tcaatggagg aatcgggaac cagtgcggtt
     3781 gcaatggcgg ctgctactgc acaagctcct gagatagcaa cgctggtaaa atgtgttggt
     3841 gtacagacga taatgctgtc taaaattgcc tgttgaatgt ttcctggatt gatgagacca
     3901 atgggagaaa tgcgcatggc accaccgtta gtatctccca gaacgccggt ttcatgcggg
     3961 tccaccccgc cctttaattg taaacaagct tttcttgtag atggtccaac atgcggattg
     4021 tgatcaccat ctatcagatc gtaccaacca atgattgctt ttgcagctaa ctcaacgcta
     4081 attttcttat taattaatat ttcttttatg atggacatag cctgctgtgt atcgtctgtt
     4141 acttctccgg gtgccaaccc agcgtgaact gggtgatcag aaggtcctgg taaaaaatcg
     4201 ttaatccacc caccatagaa atcccaggta tcttttggtt taaaatacgc aggcatagcg
     4261 aacgcatcac cgagggcttg ccctatcaac cctccatata catgttcaaa caaatacttt
     4321 ggatctaatt tgctgtactc ttttaatgac atttgtaaat acttgtaaac ggatgtgttt
     4381 aagtggttaa gatatgttga caattatacc tataatctct ataatttcaa taaaaaggag
     4441 gtaaacagta tatcgaagac gaaagttaat tttattaata gacagaattg acaatttaaa
     4501 actttgaaat aaggagttgc acgatgagga agtttattag ttttgtgatg atcctagtgg
     4561 ttttgtttgt atcaacgagt tgtgtttcat ctaaagcccc cgaaacagaa gtagccgaaa
     4621 tagaagcccc cgaagaagta cccgcacaag gcgctgtgat tgcagaatgt atttctgcga
     4681 cagccagtat tgatgaccga tctttttgcc aaggtgcatg ggagggcatt aaggcttttg
     4741 gcgatgagaa tggattacca tacgcttatt atcaggcgac cggtgattca accacagatt
     4801 tggtcaattg cattggtgtt gcagtcgatg gtggtgcgga aatcgtggta gtgcccggct
     4861 ttgcttgggg tgcaccagta tttttagcac aggatcttta tcccgatacc aaattagtta
     4921 tattggatgg agaccctcac agtgaagact attctgaata tcggcaagaa gataatgtct
     4981 attcgattta ttatgccgaa caagaatcag gattcctggc tggttatgcc gccgtaaaag
     5041 aaggtttcac aaacctgggt ttcatcggcg gtatggcagt gcctccagtg gttcgttttg
     5101 gttacgggtt tgtggctggc gcggagtacg ccgctcagga gatgggtctt gaagagggct
     5161 ccataaccat gtattaccat tacctgggta acttcgacgt ttcacctgaa aaccagacct
     5221 atgcagcctc atggttcagc agtggtatcg acattatctt tgctgctgcg ggtgatgtga
     5281 ctagtgttgt gagttctgct gcgtcacaaa cgggcgaggg taaatgggtt atcggcgttg
     5341 atgttaatca gggctgggat gcagacaatg tgcttacttc tgcaacaaaa aatctgcgtg
     5401 gttcaaccta tcaagctctt gacctgtact tcaatggtga atttccaggt gggcaggttg
     5461 cgaccctcaa ttcttcaaat gagggcgtga atatcccgac tgatgaggaa acatggagat
     5521 tctcaacgtt taccatggaa gagtacgaag ccattctcag caaactcagt gcagatgaag
     5581 gcggaatagc ctcaagtatt cccgttgata caggttatgc aacagcagat ttgattcctc
     5641 tacgatatgt tgttgtcacg gtttactaat cagtaagata gacaaaaaga agagttaatc
     5701 agtcaaaaat taggagaggt aatccttgta taaatgcctc tcctaataaa aaaatattca
     5761 tgatggaatt atcctgacca tatattctgg caagagttct ttgacaaaaa taaattattg
     5821 gtagagcaga cgagagaata atacttcagc aggcaaaaat cactgtggca aaaccttaag
     5881 aataccaaac gtattttcat ccataatctc attgagaaca attaagactg attttatgaa
     5941 atctggttga aatatttgat gaaaatattg atttggattc ataagataga aattattcgt
     6001 agaaaaaatt gaagactttt ccttgcttat gacaaggaaa aggacgattc ttagattctt
     6061 gagtggagag gtgaaatgga atatgctgtt gagttgttga atattaaaaa acaatttcct
     6121 ggagtcttgg cgaacgatca tattactctg aaaataaaaa agggtgaaac tcacgcactc
     6181 ttaggagaaa atggtgcggg gaaatcaact cttatgagta ttctgttcgg tctgtactca
     6241 tctgatgaag gtacgatcaa gattaatgga aaagaagtca aaataaccaa tccgaacgat
     6301 gcgaatgctt tgggcattgg gatggtgcac cagcatttca agctggtaca taattttact
     6361 gttctggaaa acattatttt aggcgtggaa tcgaccaaga ataagttatt ggacatggct
     6421 tctgccagag caaaagtgat ggaccttagc aaacggtatg gtcttgacat tgatccagat
     6481 aagataattt cagaaatcac tgtcggcatg cagcaaagag tagaaatact gaaaatgctt
     6541 taccgtgcca atgatattct gatctttgat gagcccacat ctgttctact gccgcaagag
     6601 gttgacgaac tgataaaaat cataaaggga ttggctgcgg aaggcaaaac gatattattc
     6661 atttctcaca aacttgagga gatcaagcgg gtggctgatg tttgtacggt gttgcgtcgc
     6721 gggaaggtta tcggaacagt ggatgtcgca acaaccacaa aagaacaaat ggcagaaatg
     6781 atggtgggaa gaaaagtact gttgcaggtt gacaaaggtg atggttacaa aggcgaaaaa
     6841 agacccgttc ttgaagtgaa agatctttgt gttgaggata aagagtcagg aaaattaagg
     6901 gtagaccatg ccacctttac aattcatgcg ggagaaattg tcagccttgc gggtgttgat
     6961 aataacggtc agaagcagct tgtagaggcg atcacaggca tcataccctc aaacggtgaa
     7021 atctatttgt gtggagagaa tgttacagag cgcaatattc ggtatcgcaa tacacatggt
     7081 ttatcacata ttcccgaaga tcggcagcga catgggttgg tgatggaatt caccgtgagt
     7141 gaaaattcga tactgcaaga atactttaat gacgaatttc agaagagcgg ttttattaag
     7201 agggctgtgg tcgatcaata tgccgatgaa cttatagaag aatacgatat tcgggcagga
     7261 aggggaggag aaacaacagc aggaagttta tcaggtggga atcaacaaaa attgattctt
     7321 gcgcgtgaac tggaacgcga tcatgaactt gtgattgcag tccaaccaac tcgcggatta
     7381 gatgttggtg caacggagtt tgttcaaaag caattgatta cccaaagaaa tgcaggaaag
     7441 gcggtttttc tggtctcatt agaattggaa gaagtgatga gcttaagcga cagaatactg
     7501 gtaattcacg atgggaagat tgttgtgtcg gatcttctcc catccaagat tgatatgatg
     7561 gaattgggaa tgtacatggc tggttcaaaa ggagttgaca aaaatgcgta aaacgaaaaa
     7621 gacttgggtc gataattttc ctgatttaac cgcatcttta gtcgcatgcg ttattggctt
     7681 actggtaggt tttttggtgc ttgttgttac aaatccagaa aatagtatta aaggtttcaa
     7741 agcgctttta accggtgctg tgagcggggg atttacagat tttggtaatg ttatcgtcta
     7801 tgccggtatt tatattctct gtggtctttc agtgggattt gcgggaaaaa ctggcgtatt
     7861 caacattgga ctttccgggc aatttattat tggaggagta gctgctcttt atgttgggat
     7921 catgtggaca tggcttcccc caggtatcca ttgtctggtt gcagttatag cgggtttgct
     7981 ggcgggatta ggatggagtt caacaatcgg ttttctcaga gcttttttca atatcagtga
     8041 aattgtagtg ggaatattgt tgaactatat aggggtatat cttgcgaaca tgatcattct
     8101 ggataagttt tatgattctt atgctaaccg ctcccaggct gtgcaatcct cagcattgat
     8161 cccgacactg gggatggaaa aaatcttccc cggaaccggc gcagatatta ctttctttgt
     8221 cgcaatattg ttggcgattg tcattcatgt aattctgaat aaaacaacct ttggttatga
     8281 gatccgtact tgcggattta acaaagacgc aggtatttac agtggcatta acgcaaaaaa
     8341 gaacgttatt ctttcaatgg caatagccgg cgctcttcct ggtttagcag gggcgatgtt
     8401 ttatttgtct tcttctcata actatattca gattgtagat tcggctccag ccattccgat
     8461 gagaggtatt gtcgtttcgc tggttgccat gggcagcccg atagggatta tcttatctgc
     8521 tatattcatc gcttatatca cagtaagtgg tcttgatatg cagctttatg gctatgttcc
     8581 tgagattgtg gatgtagtaa cttcggtgat tatttactgc actgcatttg ttattcttat
     8641 caaaaaagcg atttctacta ttttaagaaa atcacatgat ttgaatgaag gataaataca
     8701 atggatatcg tttattttat cgtgcaacaa cttatgttct ttatggttcc tttactcgtg
     8761 gtcgcattgg caggaatgtt ttcagagcga tctggggtgt tcaatatcgc tcttgatgga
     8821 acgatgattg tgggagcttt ttgtggtact ttgtttttaa accttaccca agattcgtgg
     8881 tcaggacaat accaattgtt ggtagcgttg ttgattgcgg gcataagcgg aattctgttt
     8941 atgctgctac atgcgttcgc ttcagtcaca ttgaatgctt cgcaaataat cagcggtatt
     9001 gctctaacat ttattgctcc ggcattagcc atatatcttg cgcgggttat ttttggcgtt
     9061 caacgaatat tcttcaccaa cgcatttctc atcagaaaaa taccaatcct gggcgatatt
     9121 ccggttattg ggaagctatt ttttaccaac acttaccttc atatttggat tggatacctg
     9181 atattgcttg tttcttatgt tgttttgtat aaaaccaaat ttggtctacg catacgtgca
     9241 tgcggtgaga acccctatgc agccgactcg gttggcatcg atgttaagaa aatgcgatat
     9301 tatggtgttc tcatctccgg tttcctggca gggatgggag ggcttatctt tatagttcca
     9361 accactatcc agttcagcgg aagtgttgca agttatggtt ttctggcaat tgcagtgctg
     9421 gtgtttgggc agtggaagcc atttaaaata ttgattgcct catttttgtt tgcattagcc
     9481 aaaacaatcg cttcatctta tacctccatc cctgctcttg ttgcgctggg ttggtcaccc
     9541 tatatctata agatgttgcc atacgtcttt acgattgtca tgcttgtact tacctccaaa
     9601 aatccgcaag ctccaaaagc tgcagggaaa ccctacataa gcggaggaga atagagattt
     9661 tttatccata aaagattgtg tggaaatatt aattggcaca tgcataaaga atagaaacaa
     9721 agatccgaat ttttaaattg tgaactgttc acttcctctt caaagaagat ccggtatatg
     9781 gctttgagag atttacacgt actgcttatg agtttattta aacaaaatta aaggtgaatt
     9841 caatgaaatc gctgaggatg tatatcactg gacctcaaaa atactaccca aatggtctaa
     9901 aagagttaaa gatgatgaaa gcttatgcag agatgtatgg ctttgaagtt atcaatgatt
     9961 tttcttacat cgaaaatgga ttcagagatt atgaacacat cgatcgcaat tttttggatg
    10021 attgcgatgt cgtggtcgct gatgttaatc ctttccgcgg tggcgaaccg gaaagcggta
    10081 ccgtttttga catgggggtt gcctactcaa aaagtaaaaa gatatattca catgtcaaag
    10141 atttgcgcaa cgttgtgcat aaatatccct acgcatattt tgacgaagag ggcaacatca
    10201 ttgatgaaat gggactcatt tttcgtgccg gacaaactcc gggaaatttg atgtatatgg
    10261 ttccttcaaa gatggttgaa ggtggtttta ttgaatgtgt caaggcactg gtcaccgatc
    10321 tcaatgaaga agcgaaggat aaagagagcc gtgtaaaacc acgaatcgat tatcgcaatc
    10381 aagccacgtg gtcggtaaaa gagggtgaat atcgtgccta tttggcagga tttgaatgct
    10441 tttccaccaa tacaaaggaa gttggtgatg gcatgaaaaa aatctgcgaa aaatataatt
    10501 tccagggaat tttcccgcca gatatccctc cggattttgg tgaattatcc gaagaggggt
    10561 tcaagaatgt ttacactcgt tgtgcatatt tcttcgacag agaccagttg catattagaa
    10621 acagcaatat ggtcatcgcg aatttaaatc cttatcatgg acatgaaccc gatagtggga
    10681 cagtttttga agcggcaatg tcttatggat tggggtaccc ttgctattgc tttatcagtg
    10741 atgagcgccc cctcattgag cgaattagtt gccggaaaga tgaaaatagt atttatagag
    10801 atattgaggg atttgtggtt gaaaacttcg gttttccact ctcttcacgc ctcgctacta
    10861 ccatgaaggt cattgttgca gattttgatg aaacagccag attagtcgca aaagacctcg
    10921 gcattttcac agattgagta cgatctggga gacgcctgat tcagtttaat gttctgctaa
    10981 gcgaatcaga agaaaagatc tttaccggtt ttaaattgat gggagttggt gattttatag
    11041 ggcaagatca ccatcactcc cattactttt gtgaaaatat ttttatgtaa acagcataaa
    11101 aaagaaaagt gaagccaggc aatgaattcg ttagttaaaa ctatttcaca acaaaatcag
    11161 aatgtgaaga tcagtttcgt ttttaaatct atgtacggat tgtcccaggg aatatttctt
    11221 gtggtgtaca acctctatct aattgaattg gggattccgc tcgatacaat cgggatgatc
    11281 cttagtgcta atcccttagc tctggcggtt ggttcaatcc ccgttggata tcttacagaa
    11341 atcatcggct ttaaaaagag ctttttgctt atatatggaa tttccggttt atctttgtta
    11401 atccaaatct ttatttcaaa agttgaatgg atactattgg cagcatttgt ttgcggactg
    11461 gcgttatctg gtgattttgt agccaactta acttttttgg cacataacac aacagaaaac
    11521 gaccgggcaa aaatttatag ttacagcagt ttgtttttta atcttttcct atcaattggt
    11581 tcgttgattg ctgtagtttt gccaaacata ttgatcaatc tgggtatgtc tttattgatt
    11641 gtatatcgtc taacattgtt aatgtctgga ggtcttttaa ttgccgccat cattccagga
    11701 atgaaaattt cacaggatct ctatagtcag gaacgaaaag taagttttca accttacttt
    11761 caaggcatca atactttcac tgtaaaacag attcttacat tactgtgttt gggaatttct
    11821 ctcggagtat tgaccccctt tatgaatttg tattttattt atcatttggg aagtaacaga
    11881 gaattttttg gaacgatttc ggctttgaat gtgtttttta tttccttcgc aataatcgtt
    11941 ggtccgttga tagtgagcaa gtggagaagt gtgcctgcgg tgactgtggt cagattgctg
    12001 ttcgtgcttt ttttatgtgt attcacatat acacatagtc caatgcttgg tgcaagcgca
    12061 tactgggtta tgatgtcact gtttcaaatg tctcaaccca tatcccttgc ttttgcaatt
    12121 aaagcatcaa cgaaaaagac catgaccgcc gttacctcgt ggatcaatgt ggctttttgg
    12181 cttggatacg cgcttgcagc accggttgta gggttcttta tgtcaaaatc tcgatatcaa
    12241 catccactga tgatcgctat aatgacaatg atcctcgccg caagctttaa tcatctcttt
    12301 ttcaacaaga ttgaaaaagc tattgaaaac acaaagtagt atttccggta taagaggctt
    12361 ttcatttcct aacaacataa agggaaacac tgtcagcgtg aggtgaaaag ttctttgcat
    12421 cgcacatatc acaatccttt ataggtagac gagggaacaa tgacaaataa aattattatc
    12481 gacaccgacc cgggcattga cgatgcgctt gctatttgta tggctatcca ttctccggaa
    12541 atctctattt tgggaattac gagcgtcttt ggaaatactt atggaaagat aactgcgcag
    12601 aacgcgctac gcctttcaga gctgagtgaa caccaggtaa tccccgttgc tcgtggaagt
    12661 gatatccctc ttgttatgcc cattcaaaat cttggcacca ttgtacatgg ggaagatggg
    12721 atgggggatt ctgatctccc ctcaccccga aaagaattat catcgcaatc tgcggttgag
    12781 cttattgcaa aaataatccg cgaaaacccg ggggaaataa ctttgttgac gcttggtccg
    12841 ctgacaaata ttgcacttgc cttacgtgtt tatcctgaat tgattggtca aataaaaaaa
    12901 ctggtaataa tgggtggggt agtttcccgc ccgggaaata tgacgccagt gtccgaagcc
    12961 aatatggggc gtgatcccca tgcggctgag attgtatttg cagccgagct tccgactgtt
    13021 ctggtaggct tggatgtaac agaaaagaca atcataacaa attcgttact tgatgaaata
    13081 tttgcggtta agaatcctac gacgcgatta ttaaaaaaga tcatccccgc ttatcaacat
    13141 ttctttaaag aatattattg catggatgga tctttttacg cacatgaccc atcagcgatg
    13201 gcatatgtct taaagccgga tttatttcaa actcggaaag cccccatttt cgtagaaact
    13261 gaagggcgct gcttggggca gacgattgcg gattgggatc acaaatggga caaacggact
    13321 gaggtggagg tgtgtttgga agttgattcg cagggggttc tggatacgat aaaagccagc
    13381 ctgatcagtt aagcggtaat gataatcctt tgaaatcgtc caccgtctcg cctggcaact
    13441 ctgaccacgg ggtgcgggga aaaagttaag acgcttgccc accagcaggc gctggagcgg
    13501 ctctactttg ccaatttacg cctgctagtg gaggttgttt tgttgtgaat agaccctccc
    13561 atgctttaaa tggggaggtc tttttatctg ttcacctctg tttcgcttac cgaagcatca
    13621 tacaatgcct gcggcaaact gctccaaccg gatgccagag gcggctcata attgctgggt
    13681 atcaggaacg ctgccacttt ggttgcttga tcgtcgatcc caccggtgaa gatggggcac
    13741 gaccagtatc cgctttcgtc gatctcggtg tatggaaaat cgaacgcagg tttgagccac
    13801 caaccattgg cattgggcac atagatataa acgaccaccc gataatcatc aggattgacg
    13861 tgcagggcga taccttccag gtcatccaaa ctgccctgtg ggggtatata agtgaccact
    13921 aaattcgggt cagcgaagct ttccgcgaac ataccggaaa aagtttcttc catgccgggc
    13981 ttcaaattgc cttctccatc ccataagccc caacatgcac ctgcttctcc ttcgttgctt
    14041 actttccatg cttcatcgta cgcttcaaac cagaagaact gtacatctag catctgggcg
    14101 aaagatgtaa aactgttgaa gtatgctgct gcgttggcag gatccccatt ttttccacaa
    14161 gatggccaac ctgtttcagc aatgatgatc tgcttatcag gagaaattgc gttcactgta
    14221 ttcgtggcac ccaggtacca gtcttcgatt gtggaggttg ccttatcgat tgttacgtta
    14281 tcccagtagg ggtacacatt gaccgcaacc acgtccatgg cttcgatcag gtcaggatat
    14341 tgcttcagct ctgaccacac atcagaagtg gttaccggca catccggtac tgcttctttg
    14401 aaccaattga tatagatcat cagttctcgc tcggtgaggt caccccgcag cagggtttcg
    14461 ttcccgatga tcgccaggtc gacatctcca ttctccgcca tttcgatgag cgatgtcatc
    14521 tggctttcgt tggtggtcag atcattgctc aaccaggcac ctactgcgat gttcaacccg
    14581 aattcccggg caatcgctcc ggcattttcc aaaccgtttt ctgtcccata agttctgatc
    14641 caataagtat atggtgcgat ccgctcaata agtgctcgca gatcggcttc tgaaacggtt
    14701 gctcccatat tgggatcatc atataggtat ggtcctacat taatgccact gatgggatta
    14761 tctatatatg gcagaggcgt gggagtgatg gtaggtactg cggtttctgt tggaatggtt
    14821 gtgggggtag cgcttggaat ggttgtgggg gtagcacttg gaatgatctt cgtagcagtg
    14881 gagattgctg tagatgtggc ggtggcggtt ggcgtgcttt cggcttcttt ccctcccgtg
    14941 catgcgcaca ggagcaggat actgattgtg cagagaagca gtgcagattt tttcattttc
    15001 atctctcccg gctgaaatgt gatttaagga tggtttaatt ctagccttag tttgaaaaag
    15061 attcaactga atagaaaagt gatggatgag atacattcgt gtaggggata ggggtgtggt
    15121 agattcttta agggttgaat gtgatggagt cttgatcttg gttgacattt ttggtgcttt
    15181 gccctgcaat gtggcggcga tgactatggc aaaatatccg gatgttgatg tcgtaaccgg
    15241 actaaacctt cctatgctgc ttgaggttct gctgcaacga aacacccatt ctcttcttga
    15301 gttaaccaaa gtggctaaaa gatatggagt agaggggata aaaacaatca gcgaccttat
    15361 ttcttaaaat atgacctttg tttttgctat accaacattg agaggttttt ctttcgaaaa
    15421 cctctcaata aaaaagagat acaataagta agatatcctt cgcttgccaa acaactcctc
    15481 atttggctga ttaagaaagc ataggaagaa caaaaaattt ttattaaaag aataaagata
    15541 gccaatcaca ttggcggatc aatactgagg ttacagtatg gatattcttg tatgcattaa
    15601 gcaagttcct ggcacctctg ccgtaaagat cgacgaaaag accggttcgt tgatgcgcga
    15661 gggcattgat tccaagatga atccctatga cctgtatgcg ctggaaacag ccttccagat
    15721 cgatcaacga acggatgcgc acattacagt tctcagcatg gggccctctc cggtgcgcga
    15781 gatcatccag gaagcttttc tgatgggtga tgcggatggc gtcatcttga ccgacaggcg
    15841 tctggcgggc gcagattgtc tggcgacggc gtatgcaatc tcacagtgca tcgaaaaaat
    15901 cggattgcca gacctcatca tctgtggaaa acagaccacc gacggtgaca ctgcgcaggt
    15961 aggggcagag atcgcggaat tcttgcacat cccccatatc accaatgtat tgaagatcga
    16021 ggaaatcaaa acagaaagcc tgatcgtgga ggctgacctg ccagccatga cccaacgtgc
    16081 agaaatacga ttccctgcat tgatcacggt ggaaaagggc atctatcaac cgcgcctgcc
    16141 ttcattcaaa cgaaaacaac gtatcaacga tcaaaagatc caggtttaca gcgttcagga
    16201 cctggctgat cagaatgaag cacactatgg tttgagcgga tcccctactc aggtgctgcg
    16261 catttttcca cccgaaactg aaataaccag cgaattgtgg aaagacagcg cgcaggatct
    16321 ggctcaaaag ttctatgaca ttcttgtgga acggaaattc atctgagagg tcagcatggc
    16381 aaatatcgtg attcatcaag aaaaaatcca gaatgcggct gacttgatca acatctgtcc
    16441 ttttggagcg atccagctag aagacggagt tcttgtcatc acctctgcct gcaagatgtg
    16501 caaactgtgc atcaagaacg gtcctgtggg ggcgtttgaa ctggtcgaac agccgttgat
    16561 tgaaattgac aaaagtgcct ggcgcggaat cgcggtgtat gtggaacata ccgaaggcaa
    16621 cattcatccg gttacttttg aattgatcgg caaagcacgc gaactggcag ccaagatcgc
    16681 tcaccccgtt tatgcggttt tcgtcggcaa ccacattgaa gagaaagccc aggaactccg
    16741 ttattttggg gtcgatcggg tatatgtgta cgatcgagcg gaactgcaag acttccgggc
    16801 agagccatat gcggctgcca tggaagattt catttcgacg gtcaaacctt gcacggtttt
    16861 ggtgggcggg acgactgtag ggcgttcgct tgcgcctcgc gtggcagccc gatttcgcac
    16921 cgggcttacc gctgattgca ccaaattgga tatgcaaccc aataccgatc tcgatcagat
    16981 ccgtcccgct ttcggcggca acatcatggc gcatattcgt accaagaatc atcgaccgca
    17041 atttgcgacc gtgcgctata agatcttttc cgctccgcaa cgtttggcgc agccatttgg
    17101 tgaaatccaa tcgatggatg ttgcgcctga aaagctcaaa tccggcgtct gtgtgctttc
    17161 tattcagccc aagcagacgg ttgagaacat cgaggatgct gatgtgattg ttgctgcagg
    17221 gcgcgggatc aagaaagagc aggatctgca aatgatccgc attctggctg accgattggg
    17281 agggcaggtg gcttccacgc gccctctgat agaagctggt tgggtcgatc cgcgccgcca
    17341 gatcgggttg agtgggcgta ccgtcagccc caaattgctg atcacctgtg gtatttctgg
    17401 ttctgtgcag ttcacggcag gcatgagtgg ggctgatttt gtggtcgcta tcaatcaaga
    17461 tcctgatgcg cagatcttta aagtggcgca tataggcttg gtaggtgatg cgtaccagat
    17521 catcccggct ctattggaaa aaattgattt ccgacgaacc ggttctgtat tggacgctat
    17581 aaaaaccatc caaagcgagg tggcttgatg gaatacaaga aaattgatgc tgctgatgtt
    17641 gccgcacttg ttgctatttg cggacgggag catgtgaaaa ggcgggaggt gctcagtgaa
    17701 gatttcagcc atgacgaact ggctggtatc cgcgtgtatc ccgaagtgat ggtggaaccg
    17761 gtttccgccg aacaagtttc ggcaatcatg aaacatgcct atgagcgttg catcccggtc
    17821 accccgcgcg gtcagggtac cggcctggtg ggtggagctg tgcccgtgcg cgaagggatt
    17881 ttattaaatc tatgccagat gaataaggtg ctggaattgg atgaagccac catgatgctt
    17941 acggtggaag cgggtgtgct cttgatggat atctacaaat acgtggaacc gtatgggctg
    18001 ttctacccgg cggaccctgg agaaaagagt gccaccatcg gagggaacat caacaccaat
    18061 gccggcggta tgcgggcggt taaatatggc gtcacgcgcg attatgtaaa agggttggag
    18121 gtggttctgg cagacgggac tgttttgatg ttaagcggca agaatgccaa gaacagttcc
    18181 ggttacagcc tgaaagatct ggtcattggc tctgaaggca cgctggcagt gatcaccaag
    18241 gctattctgc gcttgattcc cctgcctcta tgggtggtga gcttgctggt gcctttcaat
    18301 gacctgaaca ctgctattcg tgcggttccc catctggcga tgttgaaagc catgcccact
    18361 tcgatagagt tcatggagcg cgaagtgatt ctggcagccg aggaatacct gggaaagaaa
    18421 ttccccgata aatcggcacc tgcctacctg ctgctcaccc tggacggaaa ttctaaaaca
    18481 gaattggatg cggcatactg cgctgctgcc gagcgctgcc tggaatgcgg tgcattggat
    18541 gtgctcatcg ctgataccga cgagcggcgt gagatgatat ggtcggcgcg cggggcgttc
    18601 ctggaagcga tcaaggcatc cacaacagag atggacgaat gcgatgtggt tgtgccacgc
    18661 gatcgaattg ccgaactggt gggcttcacc cggcagttgc agcgcgaggt caatatccgc
    18721 atcatgagct tcggacatgc cggtgatggg aatttacaca tttatgcgct gcgcgacgcc
    18781 ctctcggaag aagactggca acggaagttg gatgatattt ttgaaaagct atatgccaga
    18841 gcgaaagaat tgggtgggca ggtttcggga gaacacggca tcggatttgc caaaaagaac
    18901 tttttagctg attcgctggg agagaaaacc attgacctga tgtgtgggat caagcaggtg
    18961 ttcgatcccc gcaatattct caaccctggc aagatctgtc agtgagcgaa gctgaccgcg
    19021 atatatctcc ctccttatgg aaagcagggg aaggttgctg ggatggggtt ctagggttcc
    19081 aatctcgccg tctcctgatc tcattgagat cagcagggcg gttttcatta gccaccagcc
    19141 aacaatccag atgagctgaa gcataagcaa ttttttcatt tctagtaagg aggatgttac
    19201 cctgaacgtt gtgagggatt tcgctataat taacttcaag cgagcaattg atatctcaac
    19261 gctcacgtgg atttcaaaac gcaggcacac acggtcaagg ataaggatag tgaggtgaga
    19321 gggacagcca gattattact gcaaacagtg tgatagaata acctatgtat cggggaaggg
    19381 agggatgaac attacactca aaaacgaaat cgaaaatata gcgctggctt tagggatgat
    19441 ttccctattt ggtgaaagtg tatacgtcac tgatctttca aaaaaaggac aatttctcaa
    19501 atgaaaatag ctgttattgg gggtggatcg acttacacac ccgaattggt gaaaggcttt
    19561 cttgaccgtg cggactcatt accgcttgat gaactctggc tgatggacat tgatgcggaa
    19621 cggctggcta tcgttggtga gttcacaaag aggatggtca aagccagagg tgagcccttt
    19681 agggtagtgc tatcgcaaaa tcagcaagag gcaatagcgg attcggcgta tgtgatatcg
    19741 caattgcgtg tggggaaaat gcgcgctcgc cgcgaagatg aataccttgg tcaacgtcat
    19801 ggattgatcg gtcaagaaac gaccggtgtc ggcggaatgg cgaaagcgct gcgtactatc
    19861 ccggttgtcc tcaaaattgc agacgacatt cgtcagtcag cgcccggagc attgttcctc
    19921 aatttcgcca acccggctgg tctggtcacc gaggcgctca tgcgttatgc ccctgctatc
    19981 caggcagcag gtttgtgcaa tgtgggcatt accaccaaga tggagatcct tgaaaaactt
    20041 gaaaaagcca ccggcttttc cattgaagca gaccgtgctg ctttaaatac gctcggattg
    20101 aatcatcttt cctggcatcg cggtttcacc gtggatggtg aggagctatg gtcgaagatt
    20161 tttcctgcgt atgttgactc tctaaggaaa gaagcggaac ccgaatggga tgtacgtact
    20221 atcgaatcgt tgagcatgat ccccaattac tatctgcaat attactacta cacccacaaa
    20281 aagctggaag ctcaaaagga atggccgcct tcccgagctg aacaggtgat ggaaatcgaa
    20341 aaaagactcc tgaacgacta cgaaaacccg gcactcacgg aaccgcccgc aagtttaatg
    20401 gaacgtggcg gtgcgtacta ttctacgctt gctacgcagg tcattaactc ccactataac
    20461 gatcttggac agatccatgt aatcaacacg cgcaacgaca gcgcgatcaa tgcctggcca
    20521 tcggattggg tattggaact ccctgcgaaa gtggataaac tgggagttca cccgctttca
    20581 gcagaacctc tgcctgcggt ctgttttggc cttatctctc aggtcaaaat gtatgaactg
    20641 ctcaccgtgg aagctgctgt tcatggagat cgcaatgcag cctatcaggc attgctggcg
    20701 catcccctcg gtccgagtgc cgattgcgtg caacaggtgt tggatgatat gcttgacacg
    20761 aacaggcggt ggttacccca atttttcgag tgacgtgcca tgcaccatcc gacatgtttc
    20821 aggcggaaag gaaaaacaat gacagcaaat cctcttctta aaaaacttgg ttattctgat
    20881 acggatcgcc ttgttattat ccatgccgac gacattggca tgtgccaggc ttcgatacag
    20941 gccttcaaag atttgtggga gtttggcacg ctatcgtccg gggcggtgat ggctccctgc
    21001 ccatggttcc ttgctgcagc agagatagct cgccagaatc ccacaatgga tatgggggta
    21061 cacgctaccc tgaatgccga atgggcgacc tatcgctggg gacctctatc aacacgtgat
    21121 tcacactcag ggttgatgga tgcggaaggt tacttgcatc gttctgtgca ggcggttgtt
    21181 gaatctgcga cggttgaagc tgtaaccgtt gaagttgaaa tgcaagttga gcgcgtattg
    21241 gctgccggaa ttgatgccac gcacatcgat tcgcacatgg gtgcaatcat gcaccccaaa
    21301 ttcatccagg catacttgca agcgggtatg acccatcaac ttcccaatat gctgccccgt
    21361 gccagcgcgc atggctttga cacaatgggc attgacgaat cgacactggc atcttttacg
    21421 ccgttcttaa atgaactgga ggctaaaaaa gttcccatgc ttgatggtat gttcagcatg
    21481 ccgctggaac atgatcatga tcacatccat gttgccaagc aacttcttcg caatgtcccg
    21541 atagggatat cacatttcat tctccaccca tccgttgaca cgcctgaatt gcgcgccatc
    21601 tgccccgatt ggtcagcacg cgttgcaaat taccatgcgt tcatgagcca tgaactgaag
    21661 cagttcttga aggatgaagg aattcacttg attggatatc gtttgatcag agatgggatg
    21721 agaagttaaa aacttctcat tcaaaaaaat caacaggagc gaagatactg atgaccacaa
    21781 caaccgtgaa ggattttgca accagcacga aaactcgcat gatgtatgcg ctgatgagtt
    21841 tgggcgtggc tgtacctatt gagaccatcg gtgcatttat tctgtatttt attgtcgata
    21901 tcaagaacct gcccgccacc tggtatgcga ctttttggtt tttttatact atctacaatg
    21961 ctttgaacaa tccggcgtta gggtatgctt ctgaccgcac ccgatcgcgt tgggggaggc
    22021 ggcgaccttt tattctgttt gcaggagtac cttatgcgtt gacctttgcc ttgatcttct
    22081 gcacgccttt cgatggaaag gacaacccta tagcgctgct gatctatttt ggtgccctca
    22141 ttgtgatttg ggaaggactc tatactgcga tggcaaccgg ttattacggt ttgctccctg
    22201 aaatgttcgg tacctatcaa gaacgaaccg acgtagctgc caagatgaat attttccagg
    22261 tcattgcgct ggttctggct tctgttgtgc cacctttgtt gtggaataaa attggctggg
    22321 gagggatggc aatttcgctg gcaatcattg cgatcattcc catctacata gggttcccat
    22381 ttctgttcga acgaaaggat ttggcgaccg acaccagttt tccgtttcta aaagcactta
    22441 aattaacgtt catgaataaa agttatctca cagccgcagg tgcccaagcc atgcgttttg
    22501 tagccaccgg catcttgcag atgggcatgc ccttctacct gaagtacagc cttaaggtga
    22561 gcgacagcat gttcagcatt ttctttggtg ttgcatttct ggtttccatg ctttcgctgt
    22621 atccatggcg taattgggtg gcgaacaagc tcgactcacg acgtacactc atgttggcta
    22681 acttagtgat gattatcggc gtcattccgc ttggcttcag tccctcattt tcttttgtgc
    22741 tggcttcggc gttcatcatt ggcgtcggtt tgggcggact gatcttgatg ggtgatgtga
    22801 tcatgggtga ggtagttgat gaagatgagg tcaaaaccgg tcagcagcga gccggaatgt
    22861 acttcggtat gagcggtttc atcattacgc tcagtgggct gctcgtttct tcgatctttg
    22921 gcatcctttt gcctgctttt gggtacgata cgcttttgga agttcagccc gcctccgttg
    22981 acctgggttt tcgcattttc ctgaccgttc cctctgccat aggactcttg ttggcgatcg
    23041 tttttctcaa gctgtacccc ttgcatggga aacgcttgca ggatattcgc gatgccctgg
    23101 caaagaaaaa aacactggca gaaaagcagg gtgaaagctc tgtgtgaaag aagatcgttt
    23161 cgcacgaaaa tcgtgatcaa tagtaaaacg ggtaaagttc aaattgctgg catagcagcc
    23221 gcaaaagcgg gaaacagttt ctgcaatatt caggagggtc gttgacgagc ttttgttgaa
    23281 gtaattctat aatattttaa aatcatcagg tggaacaacg aatgaaatat tttctcggta
    23341 ttgatgtcgg ctgctcaaaa acccatgctc tcattgcgga tgaggctggt gtgtgccgcg
    23401 gttttggtaa atcttcgggc ggcaaccatc aggttgttgg ttatgctgga ctggcggctg
    23461 ttttgaagga atctttggaa caggcttgca ggatgaccaa tatcacagcg gatcaaattg
    23521 caggggcggg atttggaatt gccggttacg acttcccctc tgatcacgat ccacactggg
    23581 aagccgttgc tgcgttaggt ttatcttgcc caatagagat cgtgaacgat ggggtcaacg
    23641 ggttgctggc gggaacttct cattatgtgg gagttaacgt tacggcgggt tcgagtgtca
    23701 actgccgcgg gcgcgcccct gatggtcgaa aagggcgcat cactggaaat ggcatttctt
    23761 tcggtgaatt cggtggcggt ggcgagatag tatggaaagg gctgcacatg gttaactatg
    23821 cctggatcca gcgcatttcg cctacggcac tcacgcaaat ctatcttgaa accacagggg
    23881 cgaaggacga gatggattta atggaaggtc tgtcaaacca gacgtaccaa ctctcccccg
    23941 ttattgcggt gaagatcatg gaagcagctc tggcaggtga tgctgctgcg gggcaaatcc
    24001 tgcactgggc aggtcaagaa ctgggttggc tcgctgtttc tgtgatccgc cagattggca
    24061 tgcagaatga ggaagtggaa gtgattcttt cggggagcat cttcgatggg ggtgaactca
    24121 tcagcgatcc gatgcgtcag gttgttctcg aatatgtgcc gggtgcaaag ttgatgcgcc
    24181 tggatggacc tcctgttgct gggccgttga ttttaggcat gcaactggca gggcacgatc
    24241 cataccccct gcgttcaaag ctgatcaaaa cagctaagac gttggtggaa ccaaagagcg
    24301 ctttttaaaa attctgtgat tttgattcgt tttgctgaaa ttaaacagca gggcggcttt
    24361 ccatgtcaat atttttccca tttatgggga aggcagggaa tgtcgttctg gatactaaga
    24421 ctatatcaag ggtagttcaa gtttagaaag atgcggatgg acggagcgaa agcgctggat
    24481 aaaaggggcg taacaagaat ctgccaccca ctgatgggta tactggcaag agggtgcttt
    24541 ctaattcgaa agttatttcg aaattgaact tatcgttgct attattcatg cagtatgttt
    24601 gtaggaaaat aaagactcac aggtttttta ttcgaggccg taattggttg atcaattctg
    24661 cgttatattc atctccaccc tggcaattcc cgctccgata tactttgggt tcaattcccc
    24721 aggcctttaa ttgatcacaa gccgccagta ttaacgagtt tgagatgtaa gtatttgcca
    24781 gtgtagacaa cggacctgat ttggttccgt cagattttac ctgtacacat gcatcaccta
    24841 cgggcacatg attattgatt gtaaagtcgc acacatcact caagtgtaat ccgtcttttt
    24901 gacgtgaggg gtaatctcga tatagtaaag aagttattcc aatgacagtt gtgccaacat
    24961 cctttgcagc caatgccatt tcgattggaa acgagttgat tcccgatgtg gagacgatca
    25021 gcatagcatc attgggagta accgggtatc tttccaaaac cagatgggca taaccactca
    25081 agcgttcaat ttgactgctt ttaaccgctc cttcatgcaa catcgtactg gtctcgaaaa
    25141 ttggattgac ctgggctaaa cccccagcac gaaagaaaag ttcttcttca atcatatgag
    25201 aatgaccgca gccgaaaaca tgcaataaac catctctctt gaatacatca gctaatatct
    25261 tccctgtttg agttatcgca tcggtttctt ctaaataaat ttttttaagg atttcggcag
    25321 tattgtcata atagtcatga atgaacatgg tttgctcact tcgaaagttg gtatgcaaat
    25381 aattctacgt ggaagagata acgatcactg cggtattttg attctacgta ttcaaccact
    25441 tggttgttcg tgtcgaaagt ctgacgcttt gtcagcaaag cagcggttgg actgtctttg
    25501 cctaataact gcctttccca attggttaaa cgggcaactt caatagtttg ataagcatta
    25561 tagagtgtga gatcgcactt ggtagcaaat agtttatata aagatgctcc attgatgatt
    25621 gtttccgcga tgatttttcc aatctcttgg cgcatataac aattctcgat cgccatgggc
    25681 tctccgtcag ctaagcggag tcttcgtaga acataaacag ggtcattgat ttgaacttgt
    25741 aacttattgc tgatacagct attggcgatg attgaatcga tattgaggat gagtgaatcg
    25801 ggagttaact tccgttcgag catatcttca gagtaactgg ttaaaagggc taatttctga
    25861 ggaatctttt cgacacaaat aaatgatcct tttcccctaa cacgatagag cagtccttcg
    25921 ctttccaatt cctccaatgc cttacgaatg gtgatgcggg atacattaaa ctgttctccc
    25981 agttttcttt cggatggaat gatatcccca tgtttagaac caggttgatt gattaattcg
    26041 cgcagtgatt cctttacgtg agtggacttg ttctttttct cagttttttc agtagccata
    26101 gcttctcaaa ttatttttta gagaattcat ttctgccaat atattattaa tggtctatta
    26161 tacctgatgt tatgtatatt gtgcataagt atcggataaa tataacaagc ggtttgtata
    26221 aattggataa cgtgggtata attgtcgtta atgcacttcg atattgctaa aaaatataac
    26281 aagtggtata aattatttca ccgtaatcat tattcgataa gtacatgatc aaaaaggaaa
    26341 acattcaagc caaagttaag gcaaaggact ggagcgacgc gattcaaatt gctggtcgat
    26401 tgcttgaaat ttctgaaagt gctactcatc gttacactaa ggcgatgata actgcggtaa
    26461 aagatctggg gccttatatg gttatcaccc ctaattttgc tttcgtgcat gctgcgccct
    26521 cagaagaagt taaaaaaaat gatgttgcgt taattacact tgataaacca gtattgtttg
    26581 gttctcgcaa tgatccggta tacataatta tggctatctg ttccaaagat ggcaaatcgc
    26641 atatgaactc attggctcaa attgcaaata tattgatgca ggaaaatacg attgccaaat
    26701 tgattgaagc taaaaacgtg gaagaaattt ttaatatttt tgaaaaaaaa tgaatccata
    26761 agataatctc tcttatcaag ttttatcgag caaagagcat atgctcattg aaggagatgt
    26821 aatggaagat atcaagatac aaacagtttg cggatttggt tgcggatcgt cactgctttt
    26881 acgaatgaac attgaggagg tgctaaagga aaataattta aaagcgacag ttttttgtgg
    26941 tgatttaggt acatgtctat ctcaaccttg tgacgcaatc ttcatttccg aagagttgtg
    27001 gagccggatt gaagggcgga caacagtcac aatcgtacca attaagaact tcatggacaa
    27061 aaaagaaatt accaccaaaa caatggagtt tatgaattct ttaaactaaa aaatagaaca
    27121 agggagtgac aaaatggaaa tactaaactt cgttatcaat gaaatctttg gccaaggtgc
    27181 catattcttg gcactcattg cattaattgg cttgcttcta cagaaaaagt cctttagtga
    27241 aattgtgcgc ggaacagtga tgacagcgat tggtttcttt atccttaccc aagggacggg
    27301 aattattacc ggaaaatcga tcgatggtct ggctactgcc tttaacacta ttatgccttc
    27361 agccagtggt caacccgata tcgatatgtc ccaattcggt acagagattg gcatcgtcat
    27421 gatcatcgca ttcgcagcaa acctactgtt tgcccgtttt accaagtgga aatccgtttt
    27481 tctgacaggg catatgttat attggttccc ttatgtattc atcgcagccg gggttgatgc
    27541 tgggttgagt ggattgaaat tggttgtatt ggctgctgta gtgacttcta tttacatggt
    27601 tgtggcacct aacttaatgc gtcctttagt gaaagaggtt actggagacg atagtttcac
    27661 gattggtcat ccaactacta cattgtcagt catttcaggc tatctgggca aattgattgg
    27721 gaataaagaa aaatctactg agagcctaaa aatcccgaag agtctagatt ttctacgtga
    27781 ggtatccata actggatcgc ttgtgatctg tctaacctat atcgtaatga gctttatttt
    27841 gaaagcaaat ggttttgtgc ctggggaagt atgggggtac gcaggtggtt ctacgggcgc
    27901 ctttacctac attttcacaa agtcgattta cttcggtgtc ggcgtcactg ttatgttgct
    27961 gggtgtacgt atgcttattg ctgaaattgt tccggctttc aaagggattg ccgagaaggt
    28021 cgtccccgga gcaatacccg ctttggattg cccggttatc ttcaattacg caccaaatgc
    28081 gctgatcatc ggctttgttg ttgccatgat tacctcaact attaccatca tactcactgc
    28141 gggcacattc ccgactgtga tcatccctct gacctttact tgcttctttg agatcggtac
    28201 tgctgcgatt attggcaatg ggacgggtgg aactcgtggt gctgtggtgg gggcagccgt
    28261 ttcgggcatt atcatggtct ttctggtcgg atttggatcc tattttttca gcaataccat
    28321 caataattgg atgctcgttt atggcggtca agacttctca ttgtggggca tcataacggg
    28381 gttaattgcc aaactattgg tttgatgatc tgacgggtat ttcaatcaat aaagacagat
    28441 acgagcaatt tcgagtatct gtctttatgt ttcagaaaag ctgaacgctt ggttccattt
    28501 ccccaaaaca aggtcctccc gagtgcagtg aggatctcgc cagaccctaa ggaaccaacc
    28561 gacaagaaaa tattcgtctc ctgattgatt aaatgagagc aattcgtttt tacgaaagag
    28621 ctgttgcaca cccctttact caaaaaacac gctcaaattg cgcgggaaga gatcgagcat
    28681 atccaggaga tcgcagcggg tcagtttctc gcgcagcggt ttgggcaatt ctttcgcttt
    28741 ttttccgctc atcagcaacg ctgtttcttc ctcacagaaa tcctcgcctt cttcaatcca
    28801 acctctcata cctttgttct ccggacagat ggattggcaa tgcatgcagc ctaccagaca
    28861 attatgccat tcctttttca cccaggcagg gaagggcacc ttttttggtt tttcatccag
    28921 ataggtcagg cagcgctccg cgtgcagcag aaaacgttcc gggttgatcg ccccggtggg
    28981 gcagttcaga gtacatgcct tgcaggactt gcagcgttcc atcatcaccg gttcgcgcca
    29041 ttcatcttgc tcgcaaccta aatcggaaga gaaggatacc aggcggtgat agctgccgaa
    29101 gcctttgacg taggtgatgt tgttctttcc atattcagcc aatccgctgc acaccgccag
    29161 caattttttg gggagaaagg cttgcacgat atgatgccct tttgattcaa gcgcgcctgt
    29221 catgacatcc attattttcc ggagcttgcg ctgtgcaaag aggtaggtgg gcgggacgag
    29281 caattcgatc tttttcccgc cgtgggtgaa cgaaaagtga acgggtgggt ccttgtaagc
    29341 cacaacgatc agcgaacgcg cattgggcat tacattggag gagagatctt cgaaatcttt
    29401 caccctttct tcgtagaact cattttcaaa caggttttgt cggtactgcg agtggatggt
    29461 gttcctcaga tcaagcagat ggcgaacatc caccattttg gctcgcataa ttgaattttc
    29521 aaagagcagc atgcgatcct cccgtgagat gcgaatatgg aaaatctgga accatgaact
    29581 gattttaata gaaaaaaccc aatttcgcat tttgagcgaa aagagcaagg gcttggttca
    29641 tcacccgcag cagtgccatc catgctgcag gtgtgcaact gtgcggttgc accgtctgtc
    29701 taaccgaatc gttgaaaaag ccggaaagga aagcctgctt tttccgcttg atgattccat
    29761 actttctgtt tcgatattga ttccctcagc aggttttagt agaatgtggt taataggtgc
    29821 gcccagcctg ttttggaatg ccagaatcat tgtcgggaaa gcggtgttat tataacaatg
    29881 gcatcctgcc tctcgaaagg gcctgggtcc gaagcaacat ggatgggggt gccaaaaaac
    29941 agcaaaggag gagctatcgg atcaatccaa tcgcatcatt tttgaagtgg ctgcttcagc
    30001 caatgggacg gaaggaaagc cagtttatta tccatcattc agaactttct tttccgtcat
    30061 gaaaagaggt ctttgcggat ctcgatgagc ttgtgattca ttcaaaacag gagatatcaa
    30121 aatgttcaaa atcaatacca aaaccattgt tgccatcggt ctcggtgctg caatctttat
    30181 gttgttattc atgtatgtca aggttccgtc ccccgttccc gaaaccagct tccagaccgc
    30241 ctatgggttg agctctttcc tcgccacgtt gttcggtccc atcgccggcg ccctcatttc
    30301 cttcatcgga catgcgctca gtgatgccgt ccagtacggt tccccctggt ggagctgggt
    30361 gatcgcatcc ggcgtatctg gcttcatctt cggtttcgcc ttcaaccgca ccaaggtgga
    30421 agaaggagaa ttcaaaggca aggatatcct caccttcaac ctcatccaga tcattggtaa
    30481 cgtggtggca tgggtggtgg tggctccggt gctcgacatc ctcatctatc aggaacccgt
    30541 caacctggtc ttcacccagg gcatcacggc tgctatcatg aacatcgtca gcgcgggcgt
    30601 catcggcacc ctgctgctgg tagcttatgc cgctacccgc accaagaagg gcagtctgaa
    30661 gaaaaagtag gcgttcgcct ttctgcgatc cgcgcaaatg tcgggtcgcg atgatatcga
    30721 acaaagccgc tccgctgtgg gcggcttttt cacgcccgca cgggggagga tcaatcagca
    30781 agcgagtttc cccatggggg aaatcgttgt tatactatgg catgagcaga tgccggcgcc
    30841 tgtctcgaag gcaccatctt caggcgatcg tggtgtgaac cgtgcgcctg ctacattctt
    30901 ttgctgaaat tatcgattta tggaaaaacg agcgatcatt gatttcgacc atttcatctt
    30961 tcagtacttc agccaggcgg aacccaccct gtacgatatc aacttgaaga tctacccggg
    31021 agagaagatt ctcattgtgg gtcccagcgg cagcggcaag agcacgctgg gtcactgcat
    31081 caacgggctc atccctttct cctataaggg cgagatcacc ggcagcctga ccatcaatgg
    31141 caaggacacc cgcgaactga acgtcttcga gctctcgaaa atggtgggca cggtgctgca
    31201 ggactcagac ggacaattcg tgggtctgac cgccggggaa gatatggctt ttgcgctcga
    31261 gaatgactgc gttcggcaga acgtgatgca tgaacgggtg caaaaggtgg cgcagatggt
    31321 gggcatggaa tcgttgctga actcctcgcc cttcgagcta tccggcggac agaagcagcg
    31381 cacctcgctg gcgggcgtga tgatcgatga cgtggatatc ttgctgttcg acgaaccgct
    31441 ggcgaacctg gaccccgcta ccggcaagaa agctatcgag atcatcgatg atattcactg
    31501 tggttccgac aagacggtga tcatcatcga gcaccggctg gaggatgtgc tgtaccgcca
    31561 cgtggaccgc gtgatcgtcc tggatgaggg gcgcatcatc gccgatatgg acgcgcacga
    31621 actggtgtgt tccgacctgc tgcgcaagac cggcatccgc gaaccgcttt acatcaccgc
    31681 cctcaagtac gccggggcaa gagtgacctg cgatatgcag cccggctaca tcgccaccct
    31741 caacaccaac tcggcgggcg cggcggtgcg caactggtat aaaggcattc agccaaggca
    31801 gcgttccaat gtcaacccct ccatcatcca gatggaacac atttcttttt cgtacgatgg
    31861 cgtgcgcccg gtgctacagg atatcaactt tgacattcgc gagggcgaaa tggttgcgct
    31921 ggtgggcaag aacggcgccg gcaaatcgac gctttccaaa ttattgtgcg gatttgaaaa
    31981 ggaagataaa ggtgtcatcc gctattgcgg tgaaaacatc cgcgggaaga ccatcaagga
    32041 gcgcgccgag atcatcgggc tggtgatgca gaaccccaat cagatgatct ccaaagccat
    32101 gatttacgat gaggtggcgc tcgggctgcg cctgcgcaac atccctgaag aggagatcaa
    32161 gccacgtgtg gaaagagtgc tcaccatttg cgggctggca cccttcatcg aatggcccat
    32221 ttccgctctc agctacgggc agaagaagcg tgtgaccatc gcctccatcc tggtgctcga
    32281 ccccaggatc ctcatcctgg atgagcccac cgccgggcaa gattaccgcc actacaccga
    32341 catcatggag ttcctggtgg agatcaacaa gctgggcgtg accatcatct tgatcacgca
    32401 tgacatgcac ctgatgctgg aatatacccc gcgtgccatc gtgctggcgg gcggcaggtt
    32461 gatcgccgac acggctgctt cggtggtgct gaccgacgcg caagtgatcg cagaagccaa
    32521 ccttaaagag acctcgctct ttaatctggc gcacaggtgc ggcatcgcgg acggcacggc
    32581 tttcgtgcag ggcttcatag actatgaaag ggaactgaag aaccgatgaa caccaagatg
    32641 aagttgttct cctataacac ggtggatact tttgtgcacc gcctttcggg gctgaccaag
    32701 ctgatgtgtt tcttgctgct cactttcgcc gtgatgtact cctatgacct gcgcgtggtg
    32761 ctgctggtga tgcttttttc tttcattgtc ctcaaaatag cgcgtgtgca gttctcgcag
    32821 atccgcacca tggtgatcta tgtgctggtg ttcgtcatca ccaatgcgat catcaccttc
    32881 ttctttgccc cggagttcgg ggtggagatt tacggcacgc gccatgagat cgcgcgcgtg
    32941 gtcggcaagg tttacctcac gcaagagcaa ctgctttacc agatcaccaa gttcttcaaa
    33001 tacacctcgg tcatcccgct gggcatgatc tttctgctca ccaccaaccc cagcgaattc
    33061 gcatcgtcgc tgaacggggt aggggtgcct tataaggctg cttatgccgt ggcgctcacc
    33121 ctgcgctact tccccgacat ccagcgcaac taccacgaca tcagcctggc gcaacaggcg
    33181 cgcgggctgg acctttcgcg caaagccaag ttcggagacc gcttcaaaaa cgcgctgctg
    33241 atcatcatcc cgctcatctt ctctacgctc gatcgcattg agctgatcag caatgccatg
    33301 gacctgcgcg gcttcggcaa gagcaacaag cgcacctggt acaccttcag gcgcctgaac
    33361 cggcaggact tcgcggcgat cgtggtgtcg ctgctgatct tcgccggctc gctgtgcgta
    33421 tcggctttca tcaaccacag ccgcttctac aatccgttca tctaggagga acccgccatg
    33481 aaacccattc tggtcttcca gaccgatttc acctacaagg agggcgcggt gtgcgccatg
    33541 tacggggtgg tcaagagcgt tgaccgcgag ctggagatca tcgacggcac gcatgagatc
    33601 ccccgctatg atatctggag cgcctcgtac cggttgtacc aatccatgcg cttctggccg
    33661 cagggaacga tgttcgtctc ggtggtggac ccgggggtgg gcaccaagcg acgcgcctgt
    33721 gtggcgcgca ccaccgatgg ctacacggtg gtgacgcccg ataacggcac gctcacgcac
    33781 ctcaagcagc gcgtgggcat cacggcggtg cgcgagatcg acgagcgcac aaatcggctg
    33841 aagggcaagg gcaccgaaca gaccagcgtg ttccatgggc gcgacctgtt cgcctactgt
    33901 gcggcacgcc tggcgagcgg catcatcagc tacgaacagg tgggcaagga atacccggtg
    33961 gagaaaatcg tggcgcaggc gatattggag gcgcaggtgg agaaaggcag ggcgcgcgga
    34021 atcatcgaga tcaacgaccc caacttcggc aacctgtgga gcaacatccc gctggagctg
    34081 ttcaggcagg cgggcttcaa ttatggcgag cgactgcagg tgaccatccg ctactcgggc
    34141 aacgtggtgt acatgaacag ggtgctgttc gagcgctcgt tcggatatgc caagcggggc
    34201 gagatcatcc tatacacaaa cgaattgatg cggatagggc tggcggtgaa ccagggcaat
    34261 ttcagcgagc acttcaaaat cagctacggc gcggagtggg aagtggagat tacgaagtag
    34321 gggggagtgg caaaagttga ctattttaca aaaaataata tttattgtat tgtaaagtgt
    34381 cgttttacaa ttcaccgcga ttattgtaaa aacacgttgt tgcagtgaat gtgcgatgaa
    34441 gcgatgggtt gggatgaaat ggataggaag agatgtttcg cgtgcgctca gtatgacgta
    34501 ttgcaatgtt tgggagtgga tcactggttg ggtagtttga cctggtcgtg gtgagatccc
    34561 tcgtgttcat tcgggatgac accctctccc agccccgcaa atagggagga gattgatggc
    34621 gacatggaat gccgcactac gattccacga tttcttcctc cagcgctctc aagatggcat
    34681 ctgaccgata gatgcgtttg cgcgctctgc ccgttgtttc ttttaatatc cccagcttta
    34741 ccatcttttc aaaatagcgt tctatggtgg tgaagctgcc caattgcagg ctttcctgcg
    34801 cctgtttgat ggaggtgata ggggtgctga tcaggtagtc gaccactttc tccagattcg
    34861 cgcgcttgct ttcccctgcc aacatggtgt ggtattgctt gcgcaaggtt tgcaggtaca
    34921 ggatacgccg gctggtatct tctgcctgct ggtgcactcc ttgcaaaaag aagcgcagcc
    34981 attcctccca ttctccgcgc tggctcactg ccaacagccg ctcgtagtac tcctggcgcc
    35041 tttcctcgat gaagctgctc agtgataaca gcggctgaaa gagcagcccc catgccacca
    35101 gcagcagagt caccaacagc cgccccatgc gcccgttgcc atccagaaaa ggatggatgg
    35161 cttcaaattg gtaatggatc attccgatgc gcagtaaggg cggtagctcg cttttggcgt
    35221 gcatatattt ttccagatca gccagacata tctgcatttc ttccaccggt gggggcgcgt
    35281 agcgtgcggt ttctatggta gcacctgccc tgccgatcca gttctggctt tgccgcacct
    35341 cgcctggcgc catgctttct ccgcgcaccc cttgcatcaa cacagtgtgc atttcgcgga
    35401 tcaggcgcat gctcagaggc agatttttca agcgttccag cccaaagtcg atggctttca
    35461 catagttttg cacttcgtgg gcattttgca tatcgcggaa caggctgagt tgccccgctt
    35521 cataggaaaa gagctgctgc agggaagtgt gcgttccttc gatctgcgag gaatacaccg
    35581 cttctttgcg catgaagggc tgtgccaccg catgcggagc cgggaaagcg ttgcccacct
    35641 ccgccaaccg cgccaggctg cgatctgctt tcgatgaaat acccatcagg gtgtctgtcc
    35701 agtggatgcg gggaggcagt gctttgggca cgaatgccca gtatccctgc cgggttttga
    35761 tcacctctcc gctggcattt tttgtgaatt cacttctttg catggttttt ccataaaatg
    35821 aaaataagcg gacactgtat tttcatttta agttgataat gaaaattaaa caaggttgaa
    35881 ttttcattat ctttgattgt gaaaatcaac aacctgctac catgattgta aaagatggtt
    35941 gtgcattgat ctgatcctga ttctacttgc atctcacccc aagcagctat aaactatatc
    36001 tattacgaaa aactcgtgcg gggaaagcgc tgttttccta ttgtttttgt tctattgttg
    36061 cgttacctgc aacgcagttg ttctatactg gagggcaagc tacaatgaat cgcgctgaaa
    36121 ataaatctgc acgtcttacc caaattgagg cgctcttgat ggatcgcacc gatgggctta
    36181 cccgggctga acttgcgcgc cgcctgggtg tgcatcgctc tacgattggg cgtaacctgg
    36241 tagatatgcg tgcgcccacc tatgaagagc gcgggcgcat ctttattgat cgcagagcct
    36301 atctggtcaa cctgcgactc actttacatg aggctctctc catccatctc gccggacgtt
    36361 ttatggctac ctgcatggat cgccgcaacc cgcatgtcgc ctccatgctg cgtaaactgg
    36421 gcatttccct cgaaaaattg gcgccactca tcagccactt cgttcgttct tctgccaata
    36481 cctttgatga tgcttccaag cgccaggacc tgcactatct gcaggtgctc gaagagatca
    36541 cgcttgcctg ggctgaaaaa cgaaaagttc agctctggta tcacggggta gagggaaagg
    36601 tggtgaaaga atacattttt tgtccctatt ttatagaagt gggggcggtg gggcaggcgg
    36661 tctatgtcat cgggcatatt gaaccgcaaa gcgaaatgcg cacgttcaaa atcgagcggg
    36721 tggaacgcgt tgaagtgctg gaagaaacgt attctattcc agatgatttc gacccgcaga
    36781 acatgctgaa tcaagcctgg ggtatctggt tcaccgaaca ggaaccggta gaggtgcaac
    36841 tgcgtttttc agcgcgagtg gcggcccggg taaaagaaac ttgctggcat cccagcgaac
    36901 gggtcagcga acagggagat ggtagcctgt tgtggagcgc agagatcgct gaaccgcgcg
    36961 agatgctgcc ctgggtgcgc ggttggggtg cggatgtgga ggtgctgggg ccggaggagt
    37021 tgagagtatc aattgcgacc gaaataaaaa atcttgctga gatgtattta atgaagatta
    37081 cggagagatg aacagatgga agatttcatc gcacacgttc gccaaaaaga cggtaagtct
    37141 caattactta ctgatcatct gaacgaaaca tcagttttaa gcggtcagtt tgctgaaaaa
    37201 attggtttga aagaagcggg tgaaattatt ggtttaatgc atgatgtagg gaaggcaagt
    37261 tgtgaatttc aaaactatat tcgatctgca agtgggcaga taaatcctga tgctgatgat
    37321 tatgttgatt ctcaagagat gaaaggcaaa atagaccatt ccacagcagg ggctcaaatg
    37381 atttttcgag atttgtataa taaaggagca gctggctatt ttactgcaca agtattgtca
    37441 ctttgtcttg cctctcatca ttcaggactt attgattgtt tggctcctga tgggagtgat
    37501 gttttctcac atcgaatgga aaaatcacag aatctaaccc gcattgatga ggcaatgaag
    37561 aatgtaaaaa aaattgggat agagctgaaa ttttcttcaa attttgagaa agaccttgta
    37621 gctaagttta atagtttgca agagaccaat gattcaaaag aaacgcttac ttttaaatgt
    37681 gggcttttga tacgtttttt atttagttgt cttattgatg ccgatcgact caatactgct
    37741 gattttgaat ctcccaacaa agttccgctt agaaactatg gacaataccg tccttgggag
    37801 actcttattc agcgactaaa caaccgactg gttgatttcg agaaaaaaga gaataagaat
    37861 aaagtggatg aattgcgtaa taaggtgtcc cagtcttgtt tcaatttttc ctcaaaacct
    37921 agaggtcttt atcaattaac tgtcccgact ggcggaggga aaactttagc aagtcttcga
    37981 tttgccctga accatgtgaa aattcataat atggagagaa ttttctatat tattccttac
    38041 acatcaatta tcgatcaaaa tgcagatgaa gtcagaaaaa ttttagaaga taaagataaa
    38101 tatgggaatt atatggacag agttgttcta gaacatcatt ccaacttaac ccccgaagaa
    38161 gaaactcgta gacaaaatct cttgtcagaa aattgggatg ccccaattgt atttacaaca
    38221 caggttcaat ttttcgaagc attatttggt tttggtacaa gaggtgccag aagaatgcac
    38281 caactcgcga acgccgtgat tatttttgat gaggtgcaaa ctattcccat tcgctgtgta
    38341 catatgttca atcttgccat acgatttctt gtcaagagtt gtgaatcaac agtgatcctt
    38401 tgcactgcga ctcaacctct tttggataaa gttgacccaa ttcaaagggc attaagtatt
    38461 aggccagaac aacagataat tacaaacaca aaggaacttt ttgaagaatt aaaacgtgtt
    38521 gaggtcaatg acagaaggaa aatcggtggt tggaccaatg aagatgttgc gaatttgata
    38581 gaacaagaat tacatgacaa aggtagtgta ttggtcattg ttaataccaa gagttcagct
    38641 cgggccctgt tccaaataat aaatgaaaga aaaaataaaa atgcatatca tctcagcacc
    38701 aatatgtgtc cggcacatcg ccttgaggta ttggactgta tcaaagaaaa attatcaaaa
    38761 aagcagcccg ttatatgtat cagtacacaa ttaattgagg cgggtgtaga tattgatttc
    38821 gggtcggtga tccgttatct ggcaggactt gactcgatca cgcaagctgc tggacgatgt
    38881 aatcgcaacg gcgaacgcaa gcttgggaat gtatggattg tcaatccgag caaggaaaac
    38941 atcgaaaaac tgaaagacat caaaattggc atagaagtag ccgagcgagt tttggacgag
    39001 tttgcaaatg accctgacag atttgaaaat gacagacttg ggctaaatgc aatggaggag
    39061 tactataaat attatttcta ccaacgaaaa gaggaaatga aatataaagt tggcaaacag
    39121 tcacctgtgg gcagagatga tgatttattt aatcttctct cgttgaatac gatatctatt
    39181 gcggaatata tgaggattac taacgttgca ccggctatgc cgttcagaca gtcatttcaa
    39241 actgcatcta aagctttcaa tgtaatcgat tcatctacac gaggtgttgt tgttccttat
    39301 ggccaggaag gggaagaaat cgtgaccgag ctatgcggcg cctttgaaat caaaaagcag
    39361 tataaactgc taaaaatggc acagagatat tccgttagcc tgttccctaa tgaatttgag
    39421 atgatgacga aaaagtatgc aattcaagaa gtacaagaag gatctggaat tttttaccta
    39481 gacgatcaat actacagcca ccagttcggt tggtgtgatg aaatcgtgaa taaaatgaag
    39541 tccctaatat atcaaggagg tccctatgga taatattgta gagttcaaag tcaccggtaa
    39601 gtatgccctt tttactgacc cactgacaaa aataggaggg gaaaaatgct catatcaaat
    39661 tccaacttat gaagccctga aaggaatatt ggggtcggct tactggaaac ctaccattgt
    39721 ctggatcatc gataaggtga gggtgatgaa tgccatcagg actcaaacac agagtgctag
    39781 accggtaaat tatggcggtg ggaattcgct tgccatctat acatatttgt ccaatgtgga
    39841 atatcaggtg caagcacatt tcgaatggaa tctgtatcga gaagatatgg caaaagatcg
    39901 gaatgaaaac aaacactatt tcattgcgaa acgaatgatc gaacgaggtg gaagaaggga
    39961 tgtatttctt ggaacaagag aatgccaagg atatgtagag ccatgcaaat ttggggaggg
    40021 tgaggggttc tatgataatt atggtgagct ttccttcgac ttgatgtttc atggttttga
    40081 ttatcctgat gaaatcggca agaacgaatt ctattcacgt ttctggcggc cgcaaatgat
    40141 caacggcatt attagtttca ttcgaccgga agaatgtgcg attcgtaaat tcgtgaggct
    40201 gatgcaggct gaaccaccaa tttcaattgg cctcgaagag cagggcttgc tcgaaggcta
    40261 cgaagaaagg aggtcgtaaa atgagttgga tccaaaagct atatgagaca tacaacaata
    40321 gtcaatctat gattggcgag gaatcaaacg ataatgaagt cccgcttcta cctctttgtc
    40381 atactacgca aaaagcacag attgaaattg ttattaacgc ggaaggtgat ttccaacgag
    40441 cgagagtgat tctgaaaaat gaagctagaa cgattattcc ctgcactgag agttcgggtg
    40501 gacgtactag cggtgaagct ccacacccat tgtgcgataa attacagtat cttgcattag
    40561 attattccag tcgaactggg aatgaaaaac aatacacggc atcgtatata acccagttag
    40621 aagattggtg taattcaaaa ttttcagatt ctaaaatagc tgctgttcta aaatatatta
    40681 agaaagggtc tatcattgaa gacttaatca attatcaaat tctaattgca gcagaggatg
    40741 ggaaactttt atcaaaatgg gaaaagaaaa agaaagagga tacaccaaaa atatttgaag
    40801 tcctgtcttc acaggatgat gcatttgttc gctgggtagt tgaaattcca ggagattcag
    40861 aaacgaaggt atggcgaaat aaaactcttt gggatagatg gatgcgctac tatgcaagta
    40921 caaaaaaaga aaaatctctt tgttatgtaa ctggtgaaaa agattttaca gcagaacaac
    40981 atccagcaaa attacggaac gacggagata aggctaaact tatttcttca aatgattcgt
    41041 caggatttac tttccgagga cgtttcctta cagccaacca ggccgcagct gttggatttg
    41101 aaactacgca aaaagcgcat tttgcccttc ggtggctcat cagtcgtcag ggttatagaa
    41161 aaggagatca agcaattgtt gcgtgggcaa cttctggagt gcccataccc aaaccgacag
    41221 atgatccact gtcaattctt ggtaatgacg aattgctaac agatgaattg tcaacagtat
    41281 caactgcaca ggatgtagcg attaaactga aaaggaaaat tgctggttac ggagaagaga
    41341 ttggtgatac tacaagaatt gttgtaatgg gattggactc tgcaacaccg ggaaggctgg
    41401 cgatcaccta ttaccgtgaa ctgacaggct ctgacttctt acaaagaatc aataaatggc
    41461 acgaatcctg tgcatggact cacaattatg gatcgattga agttcgagat gaaaaaagcg
    41521 gtaaaacaaa aaacaaacat gttcctttta ttggtgcacc ggcgccatcg gatatcgcag
    41581 aagcaactta tggagtaaaa gttgctgaca agcttaaaaa tattactatt ttacgaattc
    41641 ttccttgtat tattgacgga acacaactgc cacgtgacat tgtcgaatcc tctgtaaggc
    41701 gggcatctaa tagaattagt atggaaaaat gggaatggga aaaaacactt agtatagctt
    41761 gtgcagtata taaaaaatac aaaagaaagg agaattatca aatggcactt gaagaaaaca
    41821 gaaaaacaag agattatctc tatggcaggc ttctagcact tgcagaaagt ttggagcaat
    41881 gggcattaaa tctttcgcat gaggatcgag aaacaaacgc cgcgcggttg atgcagagat
    41941 tcgccgagca cccatacagc acatggagaa caatcgaact atctcttgct ccttataaag
    42001 caagattggg aggaaaatcg ataaaacgtc agcgaatgat tgatgaagta atcgcttctt
    42061 ttaatcctga agactttatc agtgataaac gcttgagcgg tgaattttta cttgggtatc
    42121 actgccaaag ggaaatattg catccgaatt cagaacagtc agataatccc atagaaaatg
    42181 aagatgaata gaaaaggaga ataatatgga aaccttaaaa aacaagattg attttgctgt
    42241 aattgtaaat gttaaaaatg ctaatcctaa cggagatcca ttaaatggca accgcccgcg
    42301 agttaattac gatgggtatg gggaactttc tgatgtttgt atcaaacgta aaatcagaaa
    42361 tcgcttgatg gaaaccgggt attctatttt tgtacaatct gatgataatc gtatggatga
    42421 ttatcgaaat cttcgttcac gtgcagaagg ggtcttgaaa aatgtagata tgaagaacga
    42481 caagaaatat cgtgatgccg catgtaaaca gtggattgat gttcgaactt tcggtcaggt
    42541 atttgccttc agtggtaaga aaggaaaagc gggagagaag gattctgaaa acggagaggg
    42601 aacaggtgtt tcaattggta ttcgcggtcc tgtttccata cactctgcat tttctgtggc
    42661 tcctgtgctt gaccgtatat ctagtattca gataacgaaa agtgtcagtg gcgaaggcga
    42721 tggaacaaaa cgcgcttcgg atacaatggg aatgaaacat cgggttgatc atggagctta
    42781 tgtattctac ggaagtatga gtccacagct tgcaaccaaa actggctttt cagatgaaga
    42841 cgcagaagca attaaaaaaa cattgataac gttatttcgc aatgacgctt cgtctgcaag
    42901 acctgaagga agtatggaaa tctataaagt ctattggtgg aaacacaatt ctaagaatgg
    42961 tcagtactca tctgcaaaag ttcatcgatc tgtagaaaaa agaattcgtg tcaaagatgg
    43021 aattgatgaa cctcgcagta ttgatgatta cgaaattcct ggtaaggaag agattgagaa
    43081 acaattacct gggttggaat gtgaagtatt ggatggtgaa taaatgtttt ttagagtttt
    43141 accggtaagt ggaagatgct ggataataat ttgatcgaaa aatgaaaaaa cccgaatata
    43201 cagccgacga gttgttaccc ctttcaggga ttcagcattt ctatttttgt cgacgccaat
    43261 gggcattgat ccacgtcgaa cgccaatggc aggaaaacag gttgacggct gaagggcggg
    43321 cgatgcacaa aagggcggat gatccatttt tctctgaagt acgtgagggg aagatcattg
    43381 cccgctccat gcctgttgct tcttacacat tgggattatc gggggtatgt gatgtggttg
    43441 aatttatacc ttctccccag ggagtgcaat tgccgcaccg cctggaaaaa tacctgccgg
    43501 tacctgttga gtataaacgt ggaaaagaaa aaaaagatgc ctgtgatgaa gtccaattat
    43561 gcgctcaagc aatttgtcta gaagagatgc tttcaactga tatcccccaa ggctattttt
    43621 tctatggtga agtccgtcac agaattgaag tgcttttcac ggctgaactt cgtgatcttg
    43681 tgaaacgtgt ttcacaagaa atgcatcaat attttgaacg tggatatact ccgcaggtga
    43741 agccaacgaa agcctgccaa tcttgctcat tggaagatat ttgtttacct acacttcaaa
    43801 agaaaaccat ttctgccctg aaatatatcc aaacatatat tgatggagag taaatgaaaa
    43861 aattattgaa tacattgtac attacctctc cagatgcata tctggcgctg gatggagaaa
    43921 cgattttgat aaaaaaagaa gaagaagttt caaccaggct tcctctgcat aatttagaaa
    43981 atattgtctg ttttggatat cagggtgtca gcccaggttt aatggctgct tgcgctgagc
    44041 ataatattgg tttatgtttt ctttcacctc gtggatattt cttagcgcgt gtgaatggaa
    44101 aaataagagg aaatgtcttg ctgaggaaaa cgcaatatag aatttcagag gatgagatcc
    44161 gaagtgtaga aatcgcaaca tcttttttaa tggggaagat ttttaattgt cgcaaagtac
    44221 ttgatcgggc agttcgcgat cattcactgc tggttgatgt agataaacta tcggatacct
    44281 ctcaagcgct gaaagaatgt ctcaactcgg ttcacgggag caaaacgatc aatgaattga
    44341 tggggtttga gggcagtgca gccaagttgt attttggtgt ttttgatcaa ttaattcttc
    44401 aacaaaagga tgcctttcaa tttgtagaac gttctcgtag accgccgttg gataatacaa
    44461 atgcgttgct ttcgttctta tatacactgc ttgtgcatga agttacgtct gcgttggaga
    44521 cagtaggttt ggatccttat gtcggttttc tacatcaaga ccgcccaggt cgcccttctc
    44581 ttgctttgga tttaatggaa gaactccgcc cggttttcgc agatcgcctt gctctttcta
    44641 tgattaaccg aaaacaagtt tcttccgaag gttttattca aaaagaaagt ggcggcattc
    44701 ttatggatga agaaacaagg aaaaaggttt tatcaacttg gcaagaacga aaacaggaaa
    44761 cgatcaccca cccattcttg ggagaaaaga ttatgtttgg attggtccct tatgtacaag
    44821 ctttgcttct cgcacggtac ttaagaggcg atctggatgc atatccacct tttttctgga
    44881 attaggagta ggagatatga tggtcttggt aacttatgat gtcagtacaa tcaaggaatc
    44941 agggaagaaa cgtttgcata aagtagctaa acgatgtcaa aattatgggc aaagggtaca
    45001 gaattccgtt tttgaatgca ttttggatcc tggacaactg acacaattga aaaatcagtt
    45061 aatagagatc attgatatgg aagaagattc attgcgattt tattatcttg gaaatgagtg
    45121 gaagacacgg gtagaacata ttggtgcaaa aaagagcatt gaccttgaag gaccgatcat
    45181 catttagtgc attgcgaacc tgaagctaac agcgtttacg actgaggttc gcagcgttat
    45241 tttgttgatg aggaggaaaa accatctaga tttaagaaaa acaagaagaa aaagttagat
    45301 aatttgttgt taaaggctat atatggcgaa taaggataag aatccgccat atgttgcggt
    45361 cgctcccttc gtgggagcgt ggattgaaat tggagcagtt gggcatctcg tgcgaatggg
    45421 aaatgtcgct cccttcgtgg gagcgtggat tgaaatcgag caattggaat acatggaat
//
LOCUS       DAOSDS010000006        42815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_510_length_42815_cov_5.960921, whole genome shotgun sequence.
ACCESSION   DAOSDS010000006 DAOSDS010000000
VERSION     DAOSDS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 42815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42815
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_510_length_42815_cov_5.960921"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="PLL88_01500"
     CDS             <1..836
                     /locus_tag="PLL88_01500"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOJ00268.1"
                     /translation="AEILNRYAHQGVVIERDLKKRDKKLMKVFAYLEVNEKLEHNKQE
                     IEKAVFYLDKILPVPAPTYRMIRDQNWMAAWKKFYTPLEVGQRLIIIPAWVENKHEFR
                     IPICINPGMAFGTGTHPTTQLSLELLEKYVRAGSPMIDVGCGSGILSIAGILLGAAHS
                     VAVDISQAALRSIMENSILNGVQDKIESGQASVREIRSGRFSISQAPLVVANILLNIL
                     KDLFDLGMGELVSDQGLLLIAGVLTDQVKEIEEKALEKGFTRIEHITQGDWSGLAFQK
                     N"
     gene            941..1669
                     /gene="lexA"
                     /locus_tag="PLL88_01505"
     CDS             941..1669
                     /gene="lexA"
                     /locus_tag="PLL88_01505"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOJ00269.1"
                     /translation="MARKSEGLGNRHKKIMRFLIDFQNANGYSPSIREIGESINVKST
                     SLVDYYLNQLEDLGYISRENHISRSICIKKELESDQSLTAKVTGAVQKAVAALDELLQ
                     IPVMGRIVASEPIPMPASDLNYFDPESSIEIARSLLPAKEKIEDLFALEVQGDSMIDA
                     MINDGDMVIMKRTNQAQNGEMVAVWLDDKDETTLKYFYKEQNRIRLQPANPSMGPIYV
                     NNPASLRIMGKVVMVIRQVQSVAA"
     gene            complement(1692..2513)
                     /locus_tag="PLL88_01510"
     CDS             complement(1692..2513)
                     /locus_tag="PLL88_01510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ00270.1"
                     /translation="MFTKGKTVLVTGATDGIGKQTARVLAGQGAHVLVHGRSIKRVDA
                     AIAEIKREDPHAQLSGYVADFSSLQAVRELAEELITQASDLQLLINNAGVYSPEFRLS
                     QDGFELTFAVNHLAPFLLTLLLLPTLQRNAPARIINVVSIAHNVRDFNSTKIHDPSVY
                     HAWNAYKISKFANVLATYRLAEQLAGSGVTVNCLHPGAVDTKMLRSAFPATRGIGIEE
                     GAKSSIYLASSDEVQGVSGAYFEDGKPVQSAPQTYDKELQRSLWKQCEDWVGLSG"
     gene            complement(2531..3475)
                     /locus_tag="PLL88_01515"
     CDS             complement(2531..3475)
                     /locus_tag="PLL88_01515"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ00271.1"
                     /translation="MKKSIYLVLLTVLILSGCADNGQRTSELELTPYGVTPTPSAQSG
                     TVAETPTPFPTPSPAPQYHTVQAGETMSSIALLYGLDMNDVVLANPEIDPNTMVVGMQ
                     VLIPPKTADVEITNYSSTPAPIVLSRPTCEQEKSGGVWCFLTAENQNEFAVENVLVEI
                     TLGDENAAQLTAQVAAAPLNLLPSGEKLPLSAYFPPPVPQPYRFSFLLKSAIAVEDES
                     RYLSTELLEQSITISQDGLSAQISARIFVNGIAGRNVRVWLALTALDDENTIVGVRRL
                     ETKGTLGETGVMVVSGNVYSTGALIRTVELKAEAMYSQ"
     gene            3531..4187
                     /locus_tag="PLL88_01520"
     CDS             3531..4187
                     /locus_tag="PLL88_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00272.1"
                     /translation="MDMDDQNRLNTSEEQPIVEKIFPASFGQFYMSQGFRKKGVVSNE
                     CGPTSLAMIINVILNQENVPTLALRKENIIHQTNFRVWDRLPKTIPAIGGATAPWGLV
                     SAFNQWMLKLGLPWSAERYNGASRALILEKIISGKYISALKIWKNGGAHWVDIIDFSA
                     EDNVLYLLDPNPYLVHLPQNRRVQKESWDKFAEDFQRKNVWSTVLGLDREIVVYARNL
                     "
     gene            4251..7259
                     /gene="glyS"
                     /locus_tag="PLL88_01525"
     CDS             4251..7259
                     /gene="glyS"
                     /locus_tag="PLL88_01525"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOJ00273.1"
                     /translation="MSEALRFQEIILKLQDYWAKNGCLIWQPYYTQVGAGTMNPATYL
                     RVLGPEPWNVAYVEPSIRPDDGRYGENPYRLQQHYQFQVILKPDPGNPQELYLKSLQA
                     LGIDPLSHDIRFVEDNWESPALGAWGLGWEVWLDGQEITQFTYFQQAGGIVLEPVSVE
                     ITYGLERIAMALQGNYNFRTIQWNDQYTYGDVNYQGEREHSIYYFEKADVDSLRSMFD
                     LYEKEARACLENGLVLPAHDYILKCSHTFNILDTRGAVGVTERQALFSRMRDLSRKTA
                     QAYLAQREELEYPWLQKYKSTSEAQEEQLALDETLVDTPAKAQSFLFEIGTEELPAQD
                     LSDALQQLEQNTISMLDSLRLGYDSVKVSGTPRRLVIYIHELANSQSDSSEIVKGPPA
                     RAAYDANGKPTKAAEGFAKGKGLTLEDLHITEMDGGEYITAEVLHKGKAVLAVLPEAL
                     QNLIADIRFTKSMRWNASTVAFSRPIRWLVALYGNTVVPLRYANLTSGNSTRGLRFHD
                     QDSLEIKDAEQYFEFLQSQGIILDREIRKNEIVQQVSTLCRSVYASEDLDDKLVDEVC
                     NLVEAPTALLGNFSRDHLELPQEVLIDVMKKHQRYFPLLTKDGTILPNFITVRNGDDR
                     HLALVARGNEEVIDARFKDAAFFITEDRQHDLEWFRAKLGTLTFHLALGSMLNKNERI
                     EKIVSDMISMFDLSEKEIGWTKRAAHFCKADLVTNMVVEMTSLQGTIGKYYARLSGEP
                     EAVAQALEEQYFPRFAGDHIPSSRIGLMIGLADRLDSLAGLFAANMAPSGTKDPFGLR
                     RAAISIIQNLVEWNLDFDILQGLTIASKYLPLAMKTENFNACADFIRARLSHYLLEKG
                     FRYDIVAAIEAAQGNTPFRAFQAAQQLSDWVARQNWLEILQAFSRCVRITRNIPQRYA
                     VDTALFIDEEEKQLYQATQHAIKTERKAGSVDDLLNAFIPIIPAVNQFFDKVLVMTED
                     QKLQRNRLGLLQQIVGLAEHVIDFSKLEGF"
     gene            7411..9327
                     /gene="dnaG"
                     /locus_tag="PLL88_01530"
     CDS             7411..9327
                     /gene="dnaG"
                     /locus_tag="PLL88_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOJ00274.1"
                     /translation="MNNIEEIKSRIDIVDLVSETVKLRRTGKNYIGFCPFHSNTRTPA
                     FVVFPDSGTWRCFGECNEGGDIFRFLMKKEGWDFPETLRYLADKAGITLEPLTPKKQE
                     ENAYEEKLHGILEEALIFFRHHLAQTEKGRAALAYLTDKRTLTAKTIEVFELGYAPDG
                     WEDGYAYFRNKGYTEKELVDCGLITKRSEKEGFYDRFRNRIIFPIRDASQRLCGFGAR
                     ALSDEDMPKYLNSPQTLLFDKSALLYGLDKARKPIRSTDQVIIVEGYFDVIGLHQAGF
                     QNGVSPMGTALTPVQMRLLKRYTRKIILALDPDAAGEKATLKGLEVARGSMDRSEDFV
                     FDPKGLIHSEARLDADIRVCSLPAGKDPDEIALENPETWEEIIKQSKPVITHVIDTLT
                     AGKDLGDPKTKSEIAAKVVPLIKDIPNAVERDAYRQQLAHVLHIDEQALFSISETQYV
                     SRSRSRRNRAETEPSSLETLTVSEKNKLYNLEKHLILLLLKKPDMVYDLDRYLKKSTL
                     PALSTSDVESGDLQVVCALILKSLQQVDLDAEEFITRELPEDLRWLDEEREHVVREGI
                     LQERVLEDLARTVLMLRKIKVNEKIHSINMLQQSENEQRSTEIDSALQKELVDAIKTR
                     ALVEKAQSTLSIGD"
     gene            9444..9653
                     /locus_tag="PLL88_01535"
                     /pseudo
     CDS             9444..9653
                     /locus_tag="PLL88_01535"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            10161..10967
                     /locus_tag="PLL88_01540"
                     /pseudo
     CDS             10161..10967
                     /locus_tag="PLL88_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            11173..12447
                     /gene="rho"
                     /locus_tag="PLL88_01545"
     CDS             11173..12447
                     /gene="rho"
                     /locus_tag="PLL88_01545"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOJ00275.1"
                     /translation="MSIAELEKKSLGELRTMATEMGIENATTKKKEFLVVAISQYFAQ
                     QEGREAGGGILEIMPEGIGFLRQDYQMGKNDIYVSQAQLRRNELRNGDMVFGEVRPPR
                     ESERHYGLVHVESINGNPAVENEKRVRFEDLTPIFPEQRFDLETPRNVLSNRLINMIA
                     PIGKGQRGMIVSPPKAGKTTILKEVANAISTNHPEVELMISLIGERPEEVTDMDRSVE
                     GEVIASTFDEPVTSHVRTAEITLNRAKRLVEIGRDVVILMDSLTRLARAYNLVVNPSG
                     RTLSGGMDPSALYPPKKFFGAARNIENGGSLTIISTALVDTGSRLDDVVYEEFKGTGN
                     MELHLSRRLQERRIFPAIDIERSSTRREELLLSPDELQKVWLMRRMFLQMVGQPPTGG
                     GMDIAVATEAILTRLEKTKNNMEFLSTLTEGI"
     gene            12444..13544
                     /locus_tag="PLL88_01550"
     CDS             12444..13544
                     /locus_tag="PLL88_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HOJ00276.1"
                     /translation="MIKGKPDVPQEEEKPKETQGKRRNIVSIITWGVATLMILLMSAS
                     LYQYFRGESLLALFNLKSDLIHSDDSNAVLPTLAEANVYQSVKRDANADTVLPTGYRK
                     QPVTYTVQTGDSIFGIAQSFDLEPESVLWANYDLLNDDPHLISVGQDLIIPPVDGILY
                     EWQEGDTLEKVAEKYYADVEDILLFPGNDLDMTNPTIEPGALLMVPDGYRAYTTWVVP
                     VVATTDSGVNTTILGPGGCSTAAGLVGTYTFVWPTPYRVISGNDYWSGHTALDMMCYQ
                     GDAIFASDSGVVIYAGSISGGYGNMVMIDHGNGYQTVYAHLSTVNVSCGQSVYQGQVI
                     GACGSTGNSTGAHLHFETRLNGGFVNPWLVIQ"
     gene            complement(13591..16104)
                     /gene="priA"
                     /locus_tag="PLL88_01555"
     CDS             complement(13591..16104)
                     /gene="priA"
                     /locus_tag="PLL88_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOJ00277.1"
                     /translation="MNSIVRVAVNIPGLQSLYDYEVPERLSPVRKGSLVIVPFGTMRA
                     QAVVVEDNCVSRVEKRKQLLSVLDAEAVLTPQQIRLAGWMAEHFLAAISDCVHLMLPV
                     GMNQMADVLYSPTNASMETATLSSFQKRILEELSRRGPLRARQMQALFSHENWKAPVR
                     ALLRKGYLESKSVLPDPRVQRKIIRTAHGIQSPREVQERSREIGKEGTPAYERRKAAL
                     EFLLREGIPVNVAWVYAFSNANAADLKRMEEKGFIRLSEREIWRDPLEHMEVSPSVPP
                     DLTGAQSAAWNTIARRIQEKAHPAPILIHGVTGSGKTELYLRAVQAIIDTGKQVIVLV
                     PEIALTPQTVKRFMARFPGQVGLMHSRLSPGERFDTWRRARMGKIPIIIGPRSALFAP
                     LPDIGLIVVDEFHDPSFYQQEYAPAYGAVETAVQYAHQLNCPILMGSATPDITVFFKA
                     QSEGWEIIHLPDRVLAHQDYLRQKAIQLAKVTSPIQNDGKTAASLPLPLVQIVDMRHE
                     LKLGNRSIFSRQLQTALAGVLEKQQQAILFLNRRGSATYVFCRNCGKVLSCPRCDLPL
                     TLHEDKQKLICHVCGYARLVPQKCPECGMKRIGQYGSGTQKVEAELTRLFPEARVLRW
                     DADTAAQKGAEELLLSHFANHHADFLVGTQMLAKGLDLPLVTVVGIVLADVSLNFPDY
                     RAAERCFQVLTQVAGRSGRSPLGGQVILQTYQPDHFVIQRAARHDFYGFYTDEIVRRR
                     ELAYPPFSRLIRLEFRHADQLVAAKKAAELAEKIHEKIRDGKKDTELMISGPVPPFFA
                     KQRGLYRQQIILRGREPASILQDLPLEGWRIEVDPPALL"
     gene            complement(16119..16961)
                     /gene="amrB"
                     /locus_tag="PLL88_01560"
     CDS             complement(16119..16961)
                     /gene="amrB"
                     /locus_tag="PLL88_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOJ00278.1"
                     /translation="MVSDERDVRPSPIAGSWYTSNPTDLRKEIQSYLTQARIPVLERE
                     VIGLVAPHAGYRYSGPTAGYAYRAVQGNLVDVVAVFSPFHDYASESLLTTRHSAYQTP
                     LGLVPVAKELFEEFQKLINKNGLMVYETDYDREHSLEIQLPFLQTALGKEFRLFPLMI
                     RTHAMDEIEHIAQASAYVLRDKKVLLVASTDLSHFYDEQTAGKLDEEMLARIAAFSPD
                     QVLEAERRGIAFACGAGAVAAVLRTAQLLGGKTVRVLHHSTSGDETGDSSSVVGYGAA
                     VIER"
     gene            complement(17041..18255)
                     /locus_tag="PLL88_01565"
     CDS             complement(17041..18255)
                     /locus_tag="PLL88_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ00279.1"
                     /translation="MDENITSHDVEESQQIEGQKQAEESPRLLTLWDRFWKRAQKIGL
                     HEVVSRSGTILLTIVMVGLIVWIMKTFFLQEEPIAGAGGGDLYAANEASDSSELLLPA
                     YEGVSPLEGISRSSEMKTDTAVSATSRYDVVQYEVVAGDTLLGIAEKFGLDAQTVLWC
                     NYNALLDNPAAIYPGQVLNIYPTDGTTYTWQEGDGLNGVATGLHVDPEDIIEWPGNNL
                     SYETIGDYSNPNIEVGTVIFAPGGSRAYVDWSAALFVRDDAATSRILGAGQCGAVYTG
                     PIGTEAFVWPTSETYISGYEYSPEMNHWGIDIGGDTGNPIYSADAGVIVYAGWNDWGY
                     GNVIVIDHGNGWQTLYAHLNDIYVGCGDWVSYGGQLIGTMGSTGNSSGPHLHFEMSLN
                     GFRANPHKYYPY"
     gene            18367..18861
                     /locus_tag="PLL88_01570"
     CDS             18367..18861
                     /locus_tag="PLL88_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOJ00280.1"
                     /translation="MIEETLGSYLAEKGYFLATAESCTGGLIASRITNIPGSSQYFLG
                     SMITYSNQAKTRWLGVKEETLAAYGAVSRPTVEEMALGLVRNLEGFCSPAKLLTLAVS
                     GIAGPNGGTPGKPVGYVWIAWSFNTMVRSENFHFQGERISIKEQSADQALLGAWQWLQ
                     KLSQ"
     gene            complement(18865..20649)
                     /gene="metG"
                     /locus_tag="PLL88_01575"
     CDS             complement(18865..20649)
                     /gene="metG"
                     /locus_tag="PLL88_01575"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOJ00281.1"
                     /translation="MHENVLVAVAWPYANSEIHAGNLVGSYLPADIFARYHRLKGNKV
                     LMVSGSDSHGTPITVRADAEKVSSLEVYQRFHTGFLELFQQAGITYDLFTSTHTRNHF
                     HISQTIFTTLLENGYLYTEKQSQWYSTAQKRFLPDRYVEGTCYICGFEGARSDQCDRC
                     GNLLETDKLINPRSKIDGSTPKLRETEHFFLDLAKMQSEVVDFLTERQSYWRPNVIRQ
                     SLGQIQAEGLHGRPITRDLDWGIPVPLEGWEGKCLYVWFEAVIGYLSASIEWSNLEGK
                     KDAWRDWWKHQDSKTLYFIGKDNIPFHAIIWPAELIGCGEKFDELLGEEKKEKFVLPY
                     DVPANEFLNLEGKKISGSRNWAVWGKDFLTRYDPDAFRYYLAVIMPETRDADWDWEEF
                     YHRNNDELVATWGNLANRVLSFAYKYWEGVVPDPGELTELDRSLLMTIEGGFETVGKE
                     LEQVHLRAALAEIMRLATEVNKYLDQTAPWVNIKTDQPAAARAIYVAMRCIDSLKVLF
                     APFLPFTSQTLHTYFGHADQLFGSQYVQKIQDDLGEHTVLRYDPRGATGEWKPSALKA
                     GDRFLKPEPLFKKLDASIVAVEREGMGK"
     regulatory      20777..20917
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            21027..21971
                     /locus_tag="PLL88_01580"
     CDS             21027..21971
                     /locus_tag="PLL88_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOJ00282.1"
                     /translation="MNKKIVFFLSTLLIGTVLIGCSVQTSTAEMVAEVASSSISLVDG
                     LGREVTLESPATNVVSLSPSNTELLFAVGAGDQVVGRDTFSDYPAEVTNLTDVGGGYA
                     EYNLELVLELEPDLVLAAEINTVELVQSIEDLGIPVFYISNPLTFDDLYRNIENVGIL
                     TGHTDTAQIVIAEIQERVDAVVSAIAETDTTPLVFYELDATDPTKPYTPGPSSYYSTL
                     ISMAGGKNIGDALTSSWAQISLEELVIQDPDIILLGDSLWGVTPESVSEREGWNGLSA
                     VQEGMVLPFDDNLLARPGPRLIDGLEALAQTIHPEVFD"
     gene            22001..23002
                     /locus_tag="PLL88_01585"
     CDS             22001..23002
                     /locus_tag="PLL88_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOJ00283.1"
                     /translation="MFLFAGVVIAAALSIILGSVHIPFKDISSIFMGRVESGSTLPAT
                     SNKFTSILLDLRLPRTILVLLVGAALATSGGAYQGLFQNPLADPYLIGVASGAGLGSI
                     LAMTIQWPQDMLGYFAVPVSAFFGSILAVSLVYSLSRVGKTIHNTTLILAGVAVSSLS
                     NALTSFLLINAGGELRRAIVWMMGGSTLIGWKPVIASTPFILLGIGLLYSTAYQLNVL
                     QFGDEEAQQMGVPVRKVRMLIILAASLTTAAAVAFAGTIAFVGLVVPHILRRLVGNDY
                     RLLLPLSALGGAIFLCVSDILARTLLSPQEIPVGVITILFGAPFFLFILRNTKRKMW"
     gene            23006..23776
                     /locus_tag="PLL88_01590"
     CDS             23006..23776
                     /locus_tag="PLL88_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00284.1"
                     /translation="MLRVENLSVSLNKEPILKTVSFELHKGKILGLIGPNGAGKTTLI
                     RVINGSLNMDQGKVIFRKHDLADLSVQERAKFIATVPQNRTIPAGFQVRQIVLMGRTP
                     YLNWFGRCSKEDAKIAENAMRRTNTLHLADVMADELSGGEQQRVILARAFAQNTPLML
                     LDEPTTHLDIRFQMEFLDLLLEHVKEEKKSVIIAMHDLNQAMRFCDEVLLLSQGRIYA
                     DGKPQEVLTEKKLSSVYQYPIKITHGNGDTPRYIYPKL"
     gene            complement(23761..24450)
                     /locus_tag="PLL88_01595"
     CDS             complement(23761..24450)
                     /locus_tag="PLL88_01595"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOJ00285.1"
                     /translation="MQELLDEIEAIFFDIDGTLSDSDDQLVEQILKHMRFLLFLWKEP
                     KLRAFARTLVSFAMSFFNAGFHIIDRLGLDNLFMRVFPRRRPPNRQEFEKDLRLIEGV
                     EKMIKILSARYKLGIVSARSESGVMQFLKRFSLKRYFSVIVTAQTCYYTKPFPHPLIF
                     AAEQVRVPPEKCLMVGDTMADILAGKAAGMKTIGVLSGFGTFTELRKAAADFILSSPA
                     ELVSMLDQSFG"
     gene            complement(24460..25740)
                     /locus_tag="PLL88_01600"
     CDS             complement(24460..25740)
                     /locus_tag="PLL88_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOJ00286.1"
                     /translation="MTYPEYIPYKKRKRKPGRRILYLVCFMVILGIALYQIPWVNRIV
                     SWRIDLAVTYVQRLLNPVQDLPAPVVSQASTPFVPSSTPLPATSTPTPLVSPTPTAVP
                     TPIPSTVVLNPPAYDEAKDKQDWNNCGPATLALYLRYYGWSGDQYDISTVIKPTRDDR
                     NVNVEELVYYVRNYSGWLKAEFRVGGEVEIVKKLLAAGFPVMIEESFTTDRQYWPDDD
                     LWSGHYLLITGYNDTTRTFTAQDSEIGPNQSINYDILDKKWQGFNRVYIIVYREEQEQ
                     TLKAVLGEDWDVGKNRGKALAVAQQETEEDPQNVFAWFNVGTNQVYFENYLQAAQAYD
                     AALNIGWPQRMLRYQFGPFISYFHIGRTEDLLNLTSWALSISRTSEEAMLWRGWALYQ
                     NGDKDAAMSYFRDALKIRGDYQDALWAIDYINAN"
     gene            25811..27142
                     /gene="miaB"
                     /locus_tag="PLL88_01605"
     CDS             25811..27142
                     /gene="miaB"
                     /locus_tag="PLL88_01605"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOJ00287.1"
                     /translation="MFYHIWTEGCQMNVADSTRLSSALEQLGFSPTSQIEQADVIVLN
                     TCVVRQSAEDKAVGRLSSLKKAKDRKPQTVICLMGCMVGTGDYSPLKKRFPFVDVFSA
                     PSDTSRLVSFLLETERVRTNEHRSMAQAILEKEDDFILPVEMRGNTVAAFIPIVLGCS
                     HACSYCVIPLRRGKEISRPPADILADVRSLAKQGIKEVTLLGQIVDRYGRENPAYPTL
                     GELISQIHQTEGIERIRFLTSHPNWMTDELLDALSGLPKAMPHFELPIQAGDDEVLRQ
                     MRRGYTVEKYITIVKKIRSRFPFSSIATDLIVGFPGESAEQFEHSLQMLAEVRPDVTH
                     VARYSPRPGTYSAMKMQDDVSAEEKWERFRAVEAMQEKISGEINKQYLGKKVSVLFEG
                     QKKERWFGRTPTNKLVFVNSAQPLLGSVQDVRITWSGPWSMIGEIEAKELA"
     gene            27139..27846
                     /locus_tag="PLL88_01610"
     CDS             27139..27846
                     /locus_tag="PLL88_01610"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ00288.1"
                     /translation="MKKIVRFLLIGIGIVVVVVVMVFYETVFHPQRSSKVLQWLRAPE
                     EHAEWSVQARTRCGEAPFMLPTSGLIGYLWDDSFRIGHHHQGIDIFGGTAAGITPVYA
                     AYDGYLTRLSDWKSSVIMRIPDDPLQPGRQIWTYYTHMADKNGESYIVADFPPGTEEV
                     FVKAGTLLGYQGNYSGSANNPTGVHLHFSIVLSGADGNFKNELKIENTIDPSPYFGLP
                     LNGKENKGEVPVCSSNP"
     gene            27879..28682
                     /locus_tag="PLL88_01615"
     CDS             27879..28682
                     /locus_tag="PLL88_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HOJ00289.1"
                     /translation="MLQYRDLKNVLRELGVKRSEPTIAHISAALIPHVKGGAQTVLGA
                     VLASIDNILTPSFTYKTMLVPEVGPDDNLLEYGSGRLENLNASIYTPDLPADFEDCAI
                     SEVFRHYPDVKRSSHPMLSFVGLGLDAALNAQSTTNPYAHIQYLRGKNASVLLAGKGP
                     AALFALHLCEMESGRKQFVRWAMSEEGVKECLHYPGCSQGFHKIIFHLKGKVKQTLLN
                     DQHWYAFSLDALIEVATRLLQEDPYALLCNDLHCVSCNLVRKDIRQKGM"
     gene            28762..29598
                     /locus_tag="PLL88_01620"
     CDS             28762..29598
                     /locus_tag="PLL88_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00290.1"
                     /translation="MKEINENIVIDTTLSGVTVGVIKFPKGVIFIDSPLTPKDVQSWR
                     TTAMKNDSGPSRLHIVLDEHYDRTACVIPIKSPVLAHEKTSKAIQSRPTFFRFPTSDT
                     GSEWELYPEVGPMQWINPEITFTQSMKLELDDDVVELDYHPGPSRGAIWVGMPGPKII
                     FIGDTAVVEQPPFLENADIEQWLEALALLKSDQYKDYTIISGRSGVIERNAIQEQMKF
                     LSTVQKRLEKFSGKAIDSAQLEKIATSLLEEFPAKTKKIQEHFKARLKWGISQYYGRS
                     VR"
     gene            29628..30692
                     /locus_tag="PLL88_01625"
     CDS             29628..30692
                     /locus_tag="PLL88_01625"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOJ00291.1"
                     /translation="MSEQTYLPLVEFTRGAIIESIHCGVVVVSDHAGNILFKIGDENL
                     ITYLRSTAKPLQVLALLEHPEAAHYHFEDDEIAIMCASHSGTDEHVAVLKKLQQKVGI
                     DENDLQCGTHLPFDRITANQVVRGETRLSTLQHNCSGKHSGMLALAKLLAEPKESYLS
                     PDRPTQQLILQTCGEMLDIPFSRFEMGIDGCSAPVFAVPMRNAAQAYARLCQPDHLTP
                     RRTHACRCVTRAMMAHPFLVAGPERFDTAVMQSLPGKLIAKTGAEGFFGIGIMPDSIK
                     PGSPGIGIMVKICDGDLKERARPVVCMTILRHLGLLTPDVETLLKTYDRGNISNWRDI
                     AVGEVRPSTELLEALQSWKI"
     gene            30680..31393
                     /locus_tag="PLL88_01630"
     CDS             30680..31393
                     /locus_tag="PLL88_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00292.1"
                     /translation="MENITTLPNDLKERFATDQRNSQWLYQQLLATYGVPVWRKPMPA
                     IDELVSTILSQNTNDHNRDLAFDRLRHRFPTWDAICDADIEEIVDAIRPAGLGNQKGR
                     NIQAALQQIYQEHRRLDLEFLKEWEPDDIRNYLLQFKGVGFKTAAIVMQFSLEIPAFP
                     VDTHIYRVTGRFGIRPKSMNVEDCHQYMERLFKPEQYKAGHLNIIRLGREICQARKPL
                     CDRCPLRSRCAYFSEQQTK"
     gene            31453..32301
                     /locus_tag="PLL88_01635"
     CDS             31453..32301
                     /locus_tag="PLL88_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ00293.1"
                     /translation="MKVTLLLENNCLFATYLNAEHGFSAWIEDEDVKVLFDFGFSDNF
                     IRNAEALGIDLRTADYVILSHGHRDHSGGLKYLMKYYREKAMARKPIMLMTMEDIFFK
                     KYNFKKGHFSGFDVSREFLDQYFDVRITSEPVWLTKNLVYMGVTELTNDFEHKVPQIP
                     KKLVNGEWVDDVVDEDTQLAYRHKNGKETSVIAACAHYGICNIMEYAKKLTGAPQINT
                     YLGGSHLRIDEVPQSQLDKTVEYVKKQKIKDFYICHDTDLYCKQALWNATPSKEAGTG
                     LVIELE"
     gene            complement(32358..32909)
                     /locus_tag="PLL88_01640"
     CDS             complement(32358..32909)
                     /locus_tag="PLL88_01640"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HOJ00294.1"
                     /translation="MYFDSTLPEGGKTARSLWLFYAYLNQIFWRYDETSGAFLRWQDN
                     ADATTFIQAADRLTGEPLKAANVIVLFADHEVTNITMIDISLLYIKRGKALLFRDGKK
                     YDIYWTTASEEYEKTTGKLRPIRFVDAEGNPFPLKPGQTWIEIVPLFTPYWETVDSEI
                     FNRLVNGNQPGSGYWAVRVHAPE"
     gene            complement(32915..33604)
                     /locus_tag="PLL88_01645"
     CDS             complement(32915..33604)
                     /locus_tag="PLL88_01645"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOJ00295.1"
                     /translation="MKASHLITMLFLFSLLLQSCSFSTPSIPLASTGALEKSAGDSTA
                     PASPSSSPVPTLLSTPQTLGPLSFPHNVNPLNGLPVKHPENLKNPPFLVSISNFPPTA
                     RPQAGLSYCPIVFEIWIGEGMTRFLGVFYGDFPERVADAYPEDETTTTVEEPLVGPIR
                     SGRLPYESIRSLFSGYLVMASASPNVSQQISAYTNFFGNDLSDPNSAMMRVTQLEALA
                     RSNPKELGRRH"
     gene            33727..34470
                     /locus_tag="PLL88_01650"
     CDS             33727..34470
                     /locus_tag="PLL88_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00296.1"
                     /translation="MKIKTVFGIILIVMLLAACRVTPNTTATQNSAPLTSDLQPEPTG
                     SVLSDDLILRDEFDDDINKNWGMKIVSGLENQLIWSQTNGKFRLEIQPPNDTNYAFFR
                     KNTSYRDVIVQAEVQNYGQLDNAFSLMCRATEAGWYELRISSQGYYELLRYDQYLADE
                     GKNAYTNFVQTRVGSTFIKGGEETNVFALSCQGTQLKVFINGEQVYYDKRPLTIEDTT
                     YSEGTIGFGALSYGKEVDVTFNYIETLKP"
     gene            34593..35753
                     /locus_tag="PLL88_01655"
     CDS             34593..35753
                     /locus_tag="PLL88_01655"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HOJ00297.1"
                     /translation="MPLNHLDLQNQIQRFSRDELLAHENAEKQLVQASTVLEHVSHNI
                     EAMRKVGEVATTRTAIPTTELPNQAFRNAQWNDVYNLVCADGSQIMPDPHSSPIFALL
                     NIGIIRIPAEKSKAIHPVIITDLYQRDRLYIKNQLIGEDIIALDRDVLELRYLYENCQ
                     ELKGTTIALRDGLLELYHEPRSDNAFQERLQTYYAYLQSFRSAELSIAGYVDKPRSQM
                     VVELLRLCVEMEEKAHSENTYFPDINDRLLFEQRLKNGERSAIFENFLPKNDLHGTLA
                     PFPFYFFYLNVSQNEKPWIVRVEIPEWVASDKARVEVLHSALLEQCAIMGTKPYPYCL
                     HRAHETALVRQAEKEELARQIQAMQLKEGLPLDEKSYKQSAKDHTSRRIVER"
     gene            35756..36367
                     /locus_tag="PLL88_01660"
     CDS             35756..36367
                     /locus_tag="PLL88_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00298.1"
                     /translation="MNEKRIGYVLNADTLGCTAGCRIDQTTNPGFGEMITIPAQDQRI
                     IYGIIANVHILDDGLIRQLATTQIIDEHVIQDNRNNRVIPLEFSVIFLGFNQDQQIYY
                     LLPPNPPLSLESVYACSDEKSIQFTSLGSGGYLRYLVADKTTLNFDLVAAHFRHLHQI
                     LPQQNWKPWMENATRTLIHLYRNDYDTLNALLHTLSDTSIFAD"
     gene            36393..38135
                     /locus_tag="PLL88_01665"
     CDS             36393..38135
                     /locus_tag="PLL88_01665"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ00299.1"
                     /translation="MVTPTPSLPGRQIGTVIGGSLSENLVARLQISPQEIQEGSFVTI
                     KSGNWQFYGLITDLRLGATDPRFSDEQIETYFPKSISQALRNEVLYSNAIILPVLMLE
                     NGPDFDQENIKAHLAWKAQKEEAGDLMLHPIQIKTIPAHQSPVRLASAVDIATVFGNE
                     NEGNNFKIGTTREQNFPVCIDLSHFIQRSAGIFGATGTGKSFLARILLAGLINQNKAS
                     LLVFDMHDEYGFDDTASDTGEKVVGLNTYFGSKVQVVGLAGGRNIRGNSPAFQVQIAE
                     RDIEPTDIEMLAGELNLKETTATTLAALVSSFGKEWFAEFKAMKSGSMVTDEDGKKIA
                     APDSVAAWANRNGINVMAAESLHSKLGRLFTAKYISANPATNGLQQIVQLLKTGKHII
                     LSFSGYDTDLDYLFISNLITRKIREEWEKLTNLYRSEVNKASEPRQLVIVVEEAHKLL
                     NRENATQTAFSTIAREMRKYYVTLLIIDQRPSQIYDEVLSQLGTRICGYLTDDEDIRA
                     VLSGAAGKESLRNMLAHLQPKEEVLIAGWGVPMPIPVRSRRLDAAFWEDLGNRKSNVV
                     IAQDLAEQKKKLGF"
     gene            complement(38138..38608)
                     /locus_tag="PLL88_01670"
     CDS             complement(38138..38608)
                     /locus_tag="PLL88_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOJ00300.1"
                     /translation="MWTDFTQFLRQLVSNYVLASALAAWLSAQLLKLPLHYLNTKEWN
                     WMVLINPGGMPSSHSAISTATALAIGFHDGFDSSLFALAVALATIVVYDAVSIRRQAG
                     FHAEWINRIIEEILRSGDFSYKKLQEVLGHTPFEALAGITLGICTALIMRAVYY"
     gene            complement(38598..38840)
                     /gene="xseB"
                     /locus_tag="PLL88_01675"
     CDS             complement(38598..38840)
                     /gene="xseB"
                     /locus_tag="PLL88_01675"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOJ00301.1"
                     /translation="MNDGMVAQKDIKKMKYEEAMQELEKVLADLENGSLELDRMLERF
                     EFGKELLAHCQEMLEKAELRIREIKPATGNPTENVD"
     gene            complement(38837..40066)
                     /gene="xseA"
                     /locus_tag="PLL88_01680"
     CDS             complement(38837..40066)
                     /gene="xseA"
                     /locus_tag="PLL88_01680"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOJ00302.1"
                     /translation="MIDNTLFQRQIYSVSEVTGYIRVLLEENPDLQDIWVQGEISNVS
                     LPSSGHVYFTLKDESASLRCVIWKWMAQYLGFHLQDGMAVETHGKFGVYEKGGQYQFY
                     VDSIRTQGEGKLYQEFLRLKRKLEEEGLFDPQQKRTPPAFPHLIGVVTSPSGAALQDI
                     LNTLRKRFPLAEVLFSPTLVQGEGAAQNIVRALHALERAPQKPDVIILARGGGSLEDL
                     WSFNEEAVVRAVAASDIPIISGVGHETDFTLVDFAADVRAPTPTGAAVLAVPDIEELR
                     ISLQEIQVSFMQLCIENIESRRIDWKDQQFRLQHLSPERLIRQAMLQSDELAHRLHLL
                     MHNRMDLLHASLDSMRGRFRALDPQAVLQRGYALVFDQENKLVDSVAKTDKDEKIAIR
                     LKDGRLAAQVVSKYEQE"
     gene            40150..40638
                     /locus_tag="PLL88_01685"
     CDS             40150..40638
                     /locus_tag="PLL88_01685"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOJ00303.1"
                     /translation="MEHVWAPWRMKYIQETSEKGCFFCKGIALQDGADNLIVKRGKYA
                     FVMLNRYPYTSGHLMVVPYAHEASLEKLPVETKTEIMELIAQAIQVLSKEYGAEGFNV
                     GANLGLTAGAGVADHLHFHVVPRWGGDTNFMTTVGDARVLPEHLIDTQKRIQDHWVKG
                     TD"
     gene            40646..41134
                     /locus_tag="PLL88_01690"
     CDS             40646..41134
                     /locus_tag="PLL88_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ00304.1"
                     /translation="MSTVYVREKTSRDAVWIQALMLESWGSEMVVLQGRLYRPAELPA
                     FVAEQNKERCGLVTYIFDDNSCEIITLNSLQPGLGIGTQLLDAVKQSALENGCNEIRL
                     FTTNDNINALRFYQKYGFSLEELIKNGVALDRKLKPEIPLKAENGIPIRDYLRLKMGL
                     NP"
     gene            41131..41994
                     /locus_tag="PLL88_01695"
     CDS             41131..41994
                     /locus_tag="PLL88_01695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOJ00305.1"
                     /translation="MKIILKIAALAARFLPARAKAQLYRNPKLASKIRNTLNASAPRG
                     LTETWVAGGLIQGLLLNLDLQTEKDYWLGTYEIELQTAIKKYCRPGMVIYDVGANIGY
                     VSLIFAKQVGEKGKVIAFEPLPENLQRLQKNITLNGLGSRIACVQAAVVDKPGSITFL
                     VHSSGAMGKAQGSLGREEQYVAQITVPAVALDDLIFLQNYPQPDLVKMDIEGGEVLAI
                     QGMQKTLTEVQPILLIEIHSRSALDFLWTELQNCGYQLFKMERDYPHITHPSTLGEKT
                     YAVAIPKEKAG"
     gene            complement(42031..42225)
                     /locus_tag="PLL88_01700"
     CDS             complement(42031..42225)
                     /locus_tag="PLL88_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOJ00306.1"
                     /translation="MADESRKLYRSRDKRMIAGVCGGLGEYIGVDPTIVRLLFVLGLF
                     LGTLTFWVYLVMMLVIPEEP"
     gene            complement(42288..>42815)
                     /locus_tag="PLL88_01705"
     CDS             complement(42288..>42815)
                     /locus_tag="PLL88_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOJ00307.1"
                     /translation="EGHSQRVLEMTLRLAEKMGVRGDALLHIRRGSLLHDIGKIAIPD
                     SILQKAGPLTEEEWETMRKHPRIAYEMLSSIHFLEPALEIPYCHHEKWDGSGYPQGLK
                     GEEIPLSARIFAIVDVWDALSSDRPYRKAWAEKKVIEYIKEQSGGHFDPKVVEPFLAL
                     LREEKARKKTNPSKK"
BASE COUNT        11168 a        10885 c        10316 g        10446 t
ORIGIN      
        1 tagctgaaat actgaatcgt tatgcccatc agggggtggt gatcgaacgc gacttgaaaa
       61 aacgcgacaa gaagctgatg aaggtttttg cctacctgga agtcaacgaa aagttagaac
      121 acaataagca ggagatagaa aaagccgtct tttatttgga taagatcctg cctgttcccg
      181 cccccaccta tcgcatgatc cgggatcaaa actggatggc agcctggaaa aagttctaca
      241 caccgcttga agttggtcaa cgcctgatca tcatccctgc ctgggtcgaa aataaacatg
      301 agttccgaat tcccatctgc atcaaccccg gcatggcttt cggaacgggc acccacccta
      361 ccacccaact cagtttggaa ctgctcgaga aatacgtgcg ggcaggttct ccaatgatcg
      421 acgtgggctg cggctcgggc atcctttcta tcgctggcat tttgctcggc gccgcgcatt
      481 ccgttgccgt ggatatcagc caggcagctt tgcgctctat aatggaaaac agcattctga
      541 acggtgttca ggacaaaatc gagagcggtc aggcttctgt gcgagaaatt cgctcagggc
      601 gcttttccat ctcgcaagca ccgttggttg tggcaaacat cttgctgaac attctgaaag
      661 atctgtttga cctcggtatg ggtgaactgg tcagtgacca agggctcttg ctcatagcag
      721 gggtcttgac cgatcaggtc aaagagatcg aggaaaaagc gctggaaaaa ggtttcacgc
      781 gcatagaaca tatcacgcaa ggcgattggt caggtttggc ttttcagaag aattagttcc
      841 tattttttcc ctgatacgca aatttaaacc tcttgacaca gaacgtatgt tccggtatat
      901 tattagtaca tttgtccaat tatttcacga ggagcacgac atggcacgca agtcagaagg
      961 gcttggaaat cggcataaaa agatcatgcg ctttctgatc gacttccaga atgcgaacgg
     1021 gtattccccc tccattcggg agatcggcga aagcatcaat gtgaaatcca cctccctggt
     1081 tgattattat ctcaaccagt tggaagattt gggctacatt tctcgtgaaa accacatctc
     1141 gcgcagcatt tgcatcaaga aagagctcga aagtgatcaa tcgttgaccg caaaagtgac
     1201 tggcgctgta cagaaagctg ttgctgcact ggatgaactt ctccagatcc ctgttatggg
     1261 gcgcattgtc gccagtgaac ccatccccat gcccgcttca gacttgaatt attttgaccc
     1321 tgaaagcagc atcgagatcg cccgtagcct gctgcctgcc aaagagaaga tcgaagacct
     1381 gtttgccctc gaggtgcagg gtgattccat gattgatgcc atgatcaacg atggcgatat
     1441 ggtcatcatg aagcgcacta accaggcaca gaacggtgaa atggtggcag tctggttgga
     1501 tgacaaagat gaaactaccc taaagtattt ctataaagaa cagaatcgca tccgcctgca
     1561 gcccgccaac ccttccatgg gacccattta cgtcaataac cctgccagtc tgcgcattat
     1621 gggaaaagtc gtgatggtca tccggcaggt acaaagcgtg gcagcataaa aagacccgcg
     1681 tgggtctttt tttatccaga taaccccacc caatcttcac actgtttcca aagcgaacgc
     1741 tgcaattcct tatcatacgt ttgaggagca gactgaaccg gcttaccatc ttcaaagtat
     1801 gcgccgctga cgccttgcac ctcatctgag gatgccagat agatagagga tttcgcccct
     1861 tcttcaatac cgatgccccg cgtggcggga aacgcgctac gcagcatttt tgtatccacc
     1921 gcaccgggat gcaagcaatt gaccgttacg ccgctgcccg ccaattgttc cgccaaccga
     1981 taagttgcca agacattggc aaatttggag atcttgtaag cattccaggc atgatagacc
     2041 gagggatcat ggatcttggt gctattaaaa tcccgcacat tgtgggcaat ggaaacgacg
     2101 ttgataatgc gcgccggagc attgcgttgc aacgtgggca aaagcagcaa ggtaagcaaa
     2161 aaaggcgcca ggtggttcac cgcaaaggtc aattcaaagc catcctgaga taaccggaat
     2221 tcaggcgaat agacaccagc attgttgatc aatagctgaa gatcggatgc ttgggtgatc
     2281 aattcttccg ccagctcccg cactgcctgt aatgaggaaa aatcagccac gtatccgctc
     2341 aattgcgcat gaggatcttc ccgttttatt tctgctatgg cagcatcaac tctttttata
     2401 cttcgaccat gcaccaatac atgcgctccc tgaccggcaa gaaccctggc agtttgcttt
     2461 ccaataccat cggtcgcgcc ggttaccaaa actgtttttc cctttgtgaa catggatcgc
     2521 actccttggg ttattgagaa tacattgctt cggctttcaa ttcgactgtg cgaatgagag
     2581 cgccggtaga atatacattt ccactcacca ccatcacacc ggtctcacct aacgttcctt
     2641 ttgtttcaag acgtcttact ccaacaatcg tattttcgtc atctaatgcg gttagcgcta
     2701 accaaacccg cacgttcctg ccggcgatgc cgttgacaaa aatacgggca gagatctgcg
     2761 cagataatcc gtcttgtgag atcgttatgc tttgctcaag gagttcggtg gaaagataac
     2821 ggctttcatc ttccaccgca atcgccgatt tcagcaaaaa actgaaccga tacggttgcg
     2881 gaacgggcgg gggaaaataa gccgagaggg gcaatttttc gccactgggc agcaggttta
     2941 acggagcagc cgcaacttga gcggtcaatt gggcggcgtt ctcatcgccg agggtgatct
     3001 cgaccaacac attttccacc gcgaattcgt tttgattttc cgcagtaaga aaacaccaca
     3061 cgccaccgct tttttcttgc tcgcaggtcg ggcgtgaaag aacaattgga gcgggggtgg
     3121 aactatagtt cgtaatctca acatctgcgg tcttgggagg gatcagcacc tgcataccca
     3181 ctaccatggt attggggtca atttcaggat ttgccaaaac cacatcgttc atatccagtc
     3241 catagagcag tgcaatgctc gacatggttt cccctgcctg caccgtgtga tactggggtg
     3301 cgggggatgg ggtagggaac ggtgtaggcg tttctgcaac agttccgctc tgagctgatg
     3361 gtgtgggggt aacgccataa ggggttagtt ccaattcgct tgttcgttgc ccgttatcag
     3421 cacaaccact caaaatgagc actgtaagca gaacgaggta tatacttttt ttcataggag
     3481 aaaattatac tttctataat accttttata atggtaataa tcagtggtca atggatatgg
     3541 atgatcagaa ccgcttgaat acttctgagg aacaaccgat cgttgaaaag atctttcccg
     3601 cttcgttcgg tcaattctac atgtcgcagg gttttcgcaa aaaaggtgtg gtttccaatg
     3661 aatgcggtcc aaccagcctg gcgatgatca tcaacgtcat tctcaatcaa gagaacgtcc
     3721 cgacccttgc tttgcggaaa gaaaacatca ttcaccagac caattttcgg gtttgggatc
     3781 gccttcccaa aaccatcccc gccattggcg gcgcaacagc tccttggggg ctggtttcag
     3841 ctttcaacca atggatgttg aaactggggt tgccgtggag cgcagaacga tacaatggcg
     3901 cgagccgtgc tctcattctt gaaaagatca tctccggcaa atatatctcc gcattaaaga
     3961 tctggaagaa cggcggagcg cattgggttg acatcatcga tttttctgca gaagataacg
     4021 tgctctattt gcttgacccc aacccttatt tggtgcacct cccgcaaaat cgcagagtgc
     4081 aaaaagaaag ttgggacaag tttgcagaag acttccagag aaagaatgtg tggtcaacgg
     4141 tattgggtct cgaccgggag atcgtcgtct acgctcgcaa tctctaaaaa cctgcttgat
     4201 tggtataatt cgttgattag ataaaaatca tatcggtttg cggagtcatt atgagtgaag
     4261 cgctgcgttt tcaggagatc attttaaaac tgcaggatta ttgggcaaag aatggttgtt
     4321 tgatctggca gccatactac acacaggtcg gtgcgggcac catgaacccg gctacttatc
     4381 tgcgcgtgct gggacccgaa ccctggaacg tggcatatgt ggaaccctcc attcgaccgg
     4441 atgatgggcg ttacggcgag aacccatatc gcttacagca gcattaccag tttcaggtaa
     4501 tcctgaaacc agatcccggt aacccccaag agctgtacct caaatcactg caagccctgg
     4561 gcattgaccc gctcagccat gacatccgct ttgtggaaga taactgggaa tcacccgctt
     4621 tgggcgcctg gggattgggc tgggaagtct ggctggatgg tcaggagata acccaattca
     4681 cctacttcca gcaagctggg gggatcgtgc tggaacccgt ttcagtggag atcacctatg
     4741 ggttggaacg catcgccatg gcattgcagg gtaattacaa tttccgtacg atccaatgga
     4801 atgatcaata tacctacggt gacgtgaact accagggcga gcgcgaacac agcatctatt
     4861 attttgaaaa agcggacgta gacagcttgc gttccatgtt tgacctgtac gaaaaagaag
     4921 cgcgcgcctg cctggaaaat gggctggtgc tgcctgctca tgattacatc ttgaagtgct
     4981 cgcacacttt caacatcctc gatacacgcg gtgcggtagg cgtgaccgag cgccaggctt
     5041 tattttcgcg catgcgcgac ctttcacgca aaaccgcgca agcctatctg gctcagcgtg
     5101 aagaattgga gtacccctgg ttgcagaagt acaagagcac gtcagaagca caggaagaac
     5161 aactggcgct ggatgaaacc ctggtcgata ctcccgcaaa agcgcaatcg tttctctttg
     5221 aaattggcac cgaagaatta cctgcccaag atctctcaga cgctttgcag caactggaac
     5281 agaacactat ctccatgttg gattcacttc gcctgggcta tgatagcgta aaagtgtctg
     5341 gcacacctcg ccggctggtg atctatatcc atgaactcgc aaacagccag tctgattcca
     5401 gcgagattgt caaaggtcct cccgccagag ctgcctacga cgccaacggc aaacctacca
     5461 aggcggcaga aggttttgca aaaggcaaag ggttgaccct ggaagatctg catatcacgg
     5521 agatggacgg tggcgaatac atcaccgccg aagtactcca caaggggaaa gcggttctgg
     5581 cggtattgcc ggaagccttg caaaatctga tcgccgacat acgcttcacg aaatccatgc
     5641 gctggaatgc cagcaccgtt gctttttccc gtcccatccg ctggttggtg gcattgtacg
     5701 gaaacacggt ggtccctctg cgctatgcca acctcacctc cgggaacagt acacgtggtt
     5761 tacgcttcca cgaccaggat tctcttgaga tcaaggatgc ggagcagtat ttcgaattct
     5821 tgcaatcgca gggcatcatc ctcgatcgcg aaatccgcaa aaacgagatc gtccagcagg
     5881 tcagcacatt gtgcaggagc gtgtacgctt ctgaagatct ggatgacaag ctggtggatg
     5941 aggtctgcaa cctggtggaa gcgcccactg ctttgcttgg caatttttcc agagatcatc
     6001 tggaacttcc tcaagaagtg ttgatcgatg tgatgaaaaa acaccaacgc tatttcccat
     6061 tgctgacaaa ggatggaact attctgccca atttcatcac cgtccgcaat ggggatgacc
     6121 gacaccttgc gctggtggcg cgcggaaacg aagaggttat cgacgcgcgc ttcaaggacg
     6181 ctgctttctt catcaccgaa gaccggcagc acgatttgga gtggttccgc gcgaaactgg
     6241 gcaccctcac ctttcacctc gccctgggtt caatgctcaa caaaaatgag cggatagaaa
     6301 agatcgtctc cgatatgatc tccatgtttg acctgagcga gaaggaaatc ggatggacaa
     6361 agcgcgccgc ccacttttgc aaggctgacc tggttaccaa catggtggta gaaatgacat
     6421 ccctgcaggg caccattggc aaatactacg cccgtttatc cggtgagccc gaagcggtag
     6481 cgcaggcgct ggaagaacaa tattttcccc gcttcgccgg agatcacatt cccagcagca
     6541 ggatcggact gatgattggt ttggctgatc gtttggattc tttagccggt cttttcgcgg
     6601 cgaacatggc acccagcgga accaaagatc ctttcggttt acgtcgggca gccatcagca
     6661 tcattcaaaa cctggtggaa tggaacctgg attttgacat cttgcaagga ttgaccattg
     6721 cgtccaaata tctgcccctg gcgatgaaga cagaaaattt caatgcctgt gctgatttca
     6781 tcagagcccg attatcccat tacctgctcg aaaaagggtt ccgctacgac attgtagctg
     6841 ccatcgaagc tgctcaaggc aacactccct tccgggcttt tcaggctgct cagcagcttt
     6901 ccgactgggt cgcccgccag aattggttgg aaatattgca ggctttctcc cgttgcgtgc
     6961 gcatcacgcg caatattccg caacgatatg ctgttgatac cgcccttttc atagacgagg
     7021 aagaaaaaca gctctatcag gcaacacagc atgcgataaa aacggaacgc aaggcgggtt
     7081 cagtggatga tcttttgaac gctttcatcc ccatcatccc ggcagtcaac cagttctttg
     7141 acaaggtatt ggtgatgact gaagaccaaa aactacagcg caaccgcctg ggtttactcc
     7201 aacaaatcgt ggggctggca gagcatgtga ttgatttttc aaaactggag ggcttttaga
     7261 aacggctcaa acggatatct tgcccatcat tcggtcttgg gctaacaagt tccatctagc
     7321 aagataaccg ccagattcat gttcttgtta gcgtaagctg attcagttct actggcaaag
     7381 tcctataatt aatacagcag tgtggtttgc atgaacaata ttgaagagat caaatccaga
     7441 attgacattg ttgatcttgt ttccgaaacg gtaaagttac gccgtaccgg taaaaactat
     7501 atcggttttt gccccttcca ttcaaacaca cgcacgccgg ctttcgtcgt gtttcccgat
     7561 tcaggcacct ggcgctgttt tggcgaatgc aacgaagggg gggatatttt ccgttttctc
     7621 atgaaaaaag aagggtggga ttttcccgaa acgctgcgtt atctggctga taaagccggc
     7681 atcaccctcg aaccgctcac gcccaagaag caagaagaaa atgcctatga agaaaaattg
     7741 catggcattt tggaagaagc gctcatcttt ttcaggcacc atcttgcaca aaccgaaaaa
     7801 gggagagctg cacttgctta tctgacggat aaacgtaccc tcaccgccaa gaccatcgaa
     7861 gtatttgagc tgggctacgc cccggatgga tgggaagacg gttacgcata ctttcgaaac
     7921 aaagggtata ccgaaaaaga attggtggat tgcgggttga tcacgaagcg cagcgaaaaa
     7981 gaagggtttt atgatcgctt ccgcaaccgc atcatttttc ccatcagaga tgccagccag
     8041 cgcctgtgcg gttttggagc aagagctctt tctgatgaag acatgccaaa atatttgaat
     8101 tctccacaaa cgctgctctt cgataaaagc gctttgctgt atggattgga caaagcgcgc
     8161 aaacccatcc gaagcactga ccaggtcatc attgtagaag gatactttga cgtcatcggg
     8221 cttcatcaag ccggctttca gaacggggtt tcacccatgg gcactgcact gacccccgtt
     8281 caaatgcgct tgttgaaacg atatacacgc aagatcatcc tggcgctcga tcccgatgcg
     8341 gcaggtgaga aagccacgct aaaaggactt gaagttgccc gcggttctat ggaccgctca
     8401 gaagatttcg tgttcgatcc caaagggttg atccacagcg aagcgcgcct ggacgctgat
     8461 atccgcgttt gttctctacc cgcaggcaaa gacccagatg agatcgccct ggaaaacccc
     8521 gaaacgtggg aagagatcat caagcagtca aaaccggtga tcacgcatgt gatcgataca
     8581 ttgacagccg gcaaggactt gggcgatccg aaaaccaaaa gtgagatcgc cgctaaggtt
     8641 gttccactga tcaaagatat tcccaacgct gtggaacggg acgcttaccg ccagcagctt
     8701 gcccatgtgc ttcatatcga cgaacaggct ttgttctcca tctccgaaac ccagtatgtg
     8761 agcagaagcc gttccagaag gaacagggca gaaacggaac ccagctcgtt agagacactg
     8821 acggtttcgg aaaagaacaa actctacaac ctggagaagc atctcatcct tctgttattg
     8881 aaaaaacccg acatggtcta cgatctcgat cgttatctaa aaaagagcac cctccccgcg
     8941 ctttctacct ccgacgtgga atcgggcgat ctgcaggttg tttgtgcatt gatcttaaaa
     9001 tctcttcaac aggttgatct ggatgctgaa gaattcatca ccagggaact tccagaagat
     9061 ctgcggtggc tcgatgaaga gcgcgagcat gtggtgcgcg aaggaattct tcaggaacgt
     9121 gttttggagg atcttgcccg aaccgtgctc atgctgcgca agatcaaggt gaatgaaaaa
     9181 atacacagca ttaacatgct gcaacagtca gaaaatgaac aacgctctac cgagattgac
     9241 tccgccttgc aaaaagaatt ggtagatgcc ataaagacac gcgcattggt agaaaaggct
     9301 caatccaccc tgtcgattgg tgattaaatt cttcatgtat aattcaggta tgccatccta
     9361 cgcagtaaaa gaaccatcag gggatctatc ggtattcacg caattcgtaa aaatcccaac
     9421 cacgctttca acgggaatgg agtatgtctg attttataga tgacgaatcc gatgatgtga
     9481 tcgaaaatct cccagcggct ggagaaaatg atccatttga cgacctttca gaagatgctc
     9541 cgatcaaaaa tcagccagcg ctggagtatc tggaagatcc ggtgcgtttg tatttaaaag
     9601 agatcggtca aatcgaattg ttggatgcca ccaatgaatt ccatctttcc acacgcatcg
     9661 aagcggacag acgaattcgt aaattgcaaa atcaaatgtt gccaaaagat ggagaagctg
     9721 ttgatgatac cctttatgca cagatttttt tgcagatctg caaagacctt tccacctcat
     9781 ttgggaatgt ggaaagtgta acctccacca aacccggatt gacatctccc gatttcagtt
     9841 taatcctttc cgaagcgcaa atgctgcgtt cacaatggga tatcgacatc ccttcatata
     9901 cacgcgatta cctcaacaca tactggaatc agaaatatgc caccgaagat cccatcactt
     9961 cagagctgct aaccggtttg atccaggaag tatatgccat ttacttagcc agctatttgc
    10021 tgcctgccaa cgtggcgcag gctttgcttg aatttttcaa tgaaaacaaa cgcattccgc
    10081 gttctaattt tttccagaac cgtttcccca atgaaagcac gttaaaacag aatatctccg
    10141 agatccatgc cctctcgtat gacgcaacgc aggtgctcat ccgtgccaac ctgcgtttgg
    10201 tggtgagtgt agccaagaaa tatcttggca gaggcatctc gctgctggac ttgatccaag
    10261 aaggaaatct gggattactg cgcgctgtat ccaagtttga tccgaccctt ggttataaat
    10321 tcagcactta tgctacctgg tggatcaggc aatccatcag ccgccatatc gccgaacaag
    10381 cccgcaccat ccgcattccg gttcaccttt ttgagtccat ttccaaactg ctgcgtatcc
    10441 aaagaaagct cacccagagc ctgaaccgcg atccctcgtt tgaagaactt tcgctcgaat
    10501 cggaattcat cgacaagtac gatgccgacc agatcaaaga agcattacgt accgatcaac
    10561 cgatcccaaa atcattgatg gcaaaatgga aaaacgcttc acaaaaggtc cagcagatca
    10621 tgaagtcagc ggaagaacct atttcgttgg agtatccggt tggtgacgaa gatagcagcc
    10681 aactaggtga tttcatcgaa gacatggacg cggttgaacc tatggatgcc gccgctcgtg
    10741 aaatgttacg agagcaggtt caatctgcat tatcagcgct ctcagagcgg gaacgccagg
    10801 tgctggaact gcgctttggt ttattggatg gaaaagatca caccctggaa gaggtcagca
    10861 attatttcaa tgtgacccgc gaacgcatcc gccagatcga agctaaggca ttgagaaaat
    10921 tacgtcatcc ctcacgaagc cgcgacttga gagaatattt gtcataaaat ggaggatttc
    10981 ctttgcttgc aggcagccag ttaccatgtt ataatctcgc cgctgataaa aaagtgcccc
    11041 gcttgtcttt ccccttttat gatcttcatg gagcgaaaaa ctccacagga acacgcaaat
    11101 cgaacctata gtctttaacc aatatcctac tattcagtat tcataaatct atctcatggg
    11161 aaataaatta tgatttcaat tgcagaactg gaaaaaaagt cgttgggtga acttcgcaca
    11221 atggcaacgg aaatgggcat tgaaaatgcc acgacaaaaa agaaagaatt cttggtggtt
    11281 gccatttctc aatattttgc ccaacaagaa ggacgcgaag ccggtggcgg gatcttggag
    11341 atcatgcccg aagggattgg attcttgcgg caagattatc aaatgggcaa gaatgatatc
    11401 tacgtatcgc aagcgcaatt acgtcgcaac gaactgcgca acggcgacat ggtctttggt
    11461 gaagtgcgcc caccgcgcga gtcggaacgc cattatggtc ttgtgcatgt tgagagcatc
    11521 aatggcaacc cggcggtgga gaatgaaaaa agggttcgct tcgaggacct gacccccatt
    11581 ttccccgagc aacgctttga tctggagaca ccacgcaatg tgctctcaaa ccgtctcatc
    11641 aatatgattg cccccatcgg taaagggcaa cgcggtatga tcgtctcccc acccaaggct
    11701 ggaaaaacta ccatcctaaa agaggtcgcc aatgccattt ccaccaatca ccccgaagtg
    11761 gaattgatga tcagccttat tggggaacgc cccgaagaag tgaccgacat ggaccgctct
    11821 gtagagggcg aagtcatcgc ttctaccttt gacgaaccgg tcacatcgca cgttcgcacg
    11881 gctgaaatta ccctcaatcg cgcaaaacga ctggtggaaa tcggacggga tgtggttatt
    11941 ttgatggact ctttaactcg cctggcacgt gcatataacc tggtggtcaa tccttctgga
    12001 cgcacacttt ccggcggtat ggatccatcc gcgctgtatc caccgaaaaa attctttggc
    12061 gctgcccgca atatcgaaaa tggcgggtcg ctcaccatca tttcgactgc cctggtagat
    12121 accggatcgc gccttgatga tgtggtatat gaggagttca aaggaacagg gaatatggaa
    12181 ctgcatcttt cacgacgtct tcaagaacga cgcattttcc ctgccatcga tatcgaacgt
    12241 tcttccaccc gccgcgaaga attgctgctc agccccgacg agctgcaaaa agtgtggctg
    12301 atgcgccgca tgttcctgca aatggtcggg caacccccca caggaggagg catggatatc
    12361 gcagttgcga cagaagcaat tctgacccga ctggaaaaaa ccaagaacaa catggaattc
    12421 ctttccacat tgacggaggg aatttgataa aaggtaaacc cgacgttcct caagaagagg
    12481 aaaagccaaa agaaactcaa gggaaacgcc gtaatatcgt ttccataatc acctggggcg
    12541 tcgcgaccct gatgattctt ctgatgagcg cctctctcta tcaatatttc agaggagaat
    12601 cgctgcttgc tcttttcaac ctgaagtcag atctcatcca ctccgatgat tcgaatgcgg
    12661 tcttgcccac tcttgcagaa gccaatgtat atcaatccgt aaagcgtgac gccaatgcag
    12721 atacggtgct tcctaccggc tatcgcaaac agcctgtaac ttatactgtg caaaccggcg
    12781 actccatttt tggcatcgcc cagagttttg acctggaacc ggagtcggtg ctgtgggcga
    12841 actacgacct cttgaacgac gacccacacc tcatttctgt tgggcaagat ctgataatcc
    12901 cgcccgttga tggcattctg tatgaatggc aagaaggcga tacgttggag aaggttgccg
    12961 aaaaatatta cgcagacgtg gaagatattc tgctctttcc aggcaacgat ttggacatga
    13021 caaaccccac catcgaacct ggagcgctcc tcatggttcc agatgggtac cgcgcataca
    13081 ctacctgggt ggttcccgtt gttgctacga cagattcggg cgtaaacacg acaatccttg
    13141 gtcccggtgg ttgttccacg gcagccggtc ttgtaggcac atacaccttc gtctggccaa
    13201 ccccttatcg cgtcatcagt ggcaatgact attggagtgg tcacaccgct ctggatatga
    13261 tgtgctacca gggtgatgcc atttttgctt ctgactcggg agttgtgatc tatgccggtt
    13321 ctatcagcgg ggggtatggg aacatggtca tgatcgatca cgggaatggc taccaaactg
    13381 tgtatgccca cttgagcacc gtcaatgttt cctgcggtca aagcgtgtac cagggacagg
    13441 tgattggagc ctgcggttcc accgggaatt ctaccggtgc tcacctgcac ttcgagaccc
    13501 gcttgaatgg tggtttcgtc aacccctggc ttgtcatcca atagtcctga gaacaaaagg
    13561 agttggaaaa aagcggagcc cttttatctt ttacaacagg gcaggaggat ctacctcaat
    13621 gcgccaacct tctaatggca ggtcttgcag gatcgatgcg ggctccctcc cgcgcaggat
    13681 tatctgctgc cggtaaagac cccgttgttt ggcaaaaaaa ggaggcaccg gaccgcttat
    13741 catcaattcg gtgtcttttt tgccatctcg gatcttttca tggatttttt cagcgagttc
    13801 agcggctttc ttcgcagcaa cgagttgatc agcatggcga aattccagcc gaattagtct
    13861 gctaaaaggc ggatacgcca gttcgcgcct gcggacgatt tcatcagtgt aaaatccgta
    13921 gaaatcatgc ctggctgccc tttgaatcac gaagtggtct ggctggtagg tttgcaagat
    13981 cacctgtccg cccaatgggc tgcgacctga gcgcccggct acctgtgtga gcacctgaaa
    14041 acaacgctct gcagcacgat aatcagggaa gtttaaagag acgtcagcca gcacgatgcc
    14101 taccacagta accagaggaa gatccagccc ctttgccagc atttgcgtgc cgaccaggaa
    14161 atcggcgtga tgattggcaa agtgagaaag cagcaattct tccgctcctt tttgagcagc
    14221 ggtatccgca tcccagcgca aaactcgcgc ttcaggaaac agccgtgtca gctccgcttc
    14281 caccttctgt gttccacttc catattgccc gatccgcttc atcccgcact ccggacattt
    14341 ttgcggaacc aggcgcgcat aaccacaaac atggcagatg agtttttgct tatcttcatg
    14401 caaggtcaac ggaaggtcac aacgcggaca ggataacact tttccgcagt ttcgacagaa
    14461 aacataggtg gcgcttccgc gcctgttcaa gaataagatt gcctgctgtt gcttctccaa
    14521 tacccctgcc aatgccgttt gcaattgccg gctgaagatc gaccggttgc ccaacttcaa
    14581 ttcatgccgc atatccacga tttgcaccaa cggtagagga agtgatgccg cggtctttcc
    14641 atcattttgg attggtgacg tgacttttgc caattggatg gctttctgcc gcagatagtc
    14701 ctgatgcgcc aggacccgat cgggcaggtg gatgatttcc cacccttcgc tctgggcttt
    14761 gaaaaatacc gttatatccg gggtagctga tcccatcaga atagggcagt ttaactggtg
    14821 tgcgtactga acagccgttt caaccgcgcc ataagcggga gcgtattctt gctgataaaa
    14881 agaaggatca tgaaattcat ccaccacgat caagccaata tcagggagcg gcgcaaacaa
    14941 cgcactgcgg ggacctatga taatggggat ttttcccata cgtgcccgcc gccaggtgtc
    15001 aaatcgttct ccgggcgaca gacgcgagtg catcaaaccc acctgtccgg gaaaacgcgc
    15061 catgaaacgt ttgaccgttt gtggggtaag cgctatttcg ggaaccagca cgataacttg
    15121 tttgccagta tcaataatgg cttgcaccgc tctcaggtac aattcggttt ttcccgaacc
    15181 ggtcacacca tgaatgagga ttggagcagg atgtgccttt tcctgtatcc ttcgcgcgat
    15241 tgtattccag gctgcactct gcgctccggt cagatctggg ggaacagacg gagacacttc
    15301 catgtgttcc aacgggtcac gccatatttc cctctcactc aagcggataa accctttttc
    15361 ttccatcctc ttcaggtctg cagcattggc attcgaaaac gcatacaccc aggcaacgtt
    15421 caccggtatt ccctcacgca gcaagaattc aagggcagct ttccttcgtt catatgccgg
    15481 tgtcccttct ttcccgatct cccggctcct ctcctgcact tctcttggag attggatacc
    15541 atgtgcggtg cgaatgatct ttcgctgcac tcgcgggtct ggcaggacgg atttgctttc
    15601 caggtatcct ttgcgtaaaa gggcgcgcac aggcgctttc caattttcat gcgagaacaa
    15661 agcctgcatc tgccgggcgc gcaggggacc ccgccggctc aactcttcca aaatcctttt
    15721 ttgaaaagag ctgagggtag ctgtttccat tgatgcattg gttggcgaat ataagacatc
    15781 agccatttga ttcatgccca ccgggagcat gaggtgaaca caatccgaaa tggcagcaag
    15841 aaagtgttca gccatccagc cagccagccg tatttgctgg ggagtgagca ccgcttcagc
    15901 atccagaacg ctcaatagtt gttttcgctt ctccacccgt gagacgcaat tgtcttctac
    15961 gaccaccgcc tgcgcgcgca tggtcccaaa agggacgatc accagactgc cttttcgaac
    16021 aggagataat ctttcaggaa cctcgtaatc ataaaggctc tgcaaacccg gaatgttcac
    16081 cgcaacgcgc acaatggagt tcatatcacc tgctcagttc aacgttcgat gaccgccgct
    16141 ccatacccta ccacggaaga agaatcgccc gtttcatccc ctgatgtgct gtggtgcagc
    16201 acgcgaacgg ttttccctcc cagcaattga gcggtgcgca gcactgctgc taccgctccg
    16261 gctccgcagg caaaagcgat ccctctgcgc tctgcctcca atacctggtc gggggaaaac
    16321 gccgcgatcc gtgccaacat ctcttcatcc agtttaccgg cggtttgctc atcgtaaaaa
    16381 tgggaaaggt cagtgctggc aaccaggaga acttttttat ctcttaatac gtacgcggaa
    16441 gcctgagcga tatgttcgat ctcatccatc gcatgggtgc gtatcatcag tggaaacaac
    16501 cggaattctt ttcccagagc agtctgcagg aacgggagct gtatctcaag ggaatgttcc
    16561 cggtcatagt cggtttcgta caccatgaga ccatttttat tgatcagttt ttggaattct
    16621 tcaaagagct ctttagccac cggcaccaac cccaagggag tctggtaagc ggaatgtcgg
    16681 gttgttaaaa gtgattcact ggcgtaatca tgaaaaggtg aaaaaacagc caccacatcg
    16741 accagattgc cctgcacagc gcgataggca taccctgcag tcggtccaga ataacgatat
    16801 ccggcatgcg gtgcaaccaa gccgatcact tccctctcaa gcacaggaat gcgcgcctgt
    16861 gtcagatatg attgaatttc tttccgcagg tcggtagggt tgctggtata ccagcttccc
    16921 gcgatagggg atgggcgtac atctcgttca tccgaaacca tttttgcctc ctgattcaat
    16981 tatagcggga gaaggacaaa aaaagggagg cgctgcctcc ctctcatcca tgttttcaat
    17041 ctaatagggg taatacttat gcggattcgc gcgaaatcca tttaagctca tctcaaagtg
    17101 caggtgcgga ccgctggaat tgcctgtgct gcccatggtg ccaatcagtt gcccaccata
    17161 actgacccag tctccacaac ccacataaat gtcattcagg tgagcataca gtgtctgcca
    17221 accgttccca tgatcgatta cgatcacgtt accataaccc caatcattcc atccggcgta
    17281 cacgataacc cctgcgtcag cagaatagat tgggttgcca gtatccccgc cgatatcaat
    17341 accccaatgg ttcatttcag ggctgtactc gtagccagag atgtatgttt cagacgttgg
    17401 ccaaacgaaa gcttccgttc cgatcggtcc ggtataaaca gcaccacatt gaccagcgcc
    17461 aagaattctg gatgtggctg cgtcatcgcg cacaaaaagt gcggcagacc aatcaacata
    17521 tgcacgcgat ccaccgggtg caaaaatcac agttcccact tcaatattgg gattggaata
    17581 atccccgata gtctcatagc ttagattatt ccctggccat tcaatgatat cttcaggatc
    17641 cacatgcaaa ccagttgcaa cgccattcaa accatcaccc tcctgccagg tatatgtggt
    17701 tccatcggtt ggatagatat tcaacacctg cccgggataa atagccgctg gattgtccag
    17761 cagagcgtta tagttgcacc agaggacggt ttgcgcatcc agtccaaact tttccgcaat
    17821 tcccagcagc gtgtcgccag ccactacctc atattgcaca acatcatagc gcgaagtggc
    17881 agaaacagcc gtatcggtct tcatttccga agaacggctg atgccctcca gcggtgaaac
    17941 gccctcatag gcaggtagca gcagttccga actgtctgac gcttcattgg ctgcatacag
    18001 gtcaccgccg ccagctccgg caattggttc ttcctgaagg aaaaaagttt tcatgatcca
    18061 cacgatcaaa ccaaccatga cgatggtcag caagatagtg cccgatcttg agaccacctc
    18121 atgcagccct attttttgtg ctcttttcca aaagcgatcc cagagggtca ataaacgagg
    18181 ggattcttca gcttgttttt gtccctcaat ttgttgcgat tcttcaacat catgcgatgt
    18241 aatgttttca tccattcaaa cctcacgcgc catcataaca tgggcatttt tgatggtcaa
    18301 gcgcgctttg cggcatatat tttgatttcc agtgagaaaa gatataatca ccttatggaa
    18361 tccgttttga tcgaagaaac tcttgggtca tatttagctg aaaagggata ttttttggct
    18421 actgccgaat cttgtaccgg cggattgatc gccagccgca tcaccaacat ccccggatcg
    18481 tctcagtatt ttctgggaag catgatcacc tattccaatc aggcaaaaac ccgctggttg
    18541 ggagtgaagg aagaaacgct ggcggcgtac ggcgcggtga gccgaccaac cgtggaagaa
    18601 atggcgcttg ggctggtacg caacctggaa ggattttgtt ctcctgccaa actgctcacg
    18661 ctagccgttt ctggcatcgc cggaccgaat ggaggcaccc ctggcaaacc ggtaggctac
    18721 gtttggatag cctggtcatt taacacgatg gtgcgttcag aaaactttca tttccagggc
    18781 gagcgcattt ccatcaaaga acaaagcgcc gaccaggcat tgctcggcgc ctggcagtgg
    18841 ttgcagaaac tgagccaata aggcttattt tcccattcct tcgcgctcaa ccgccacaat
    18901 gctggcgtcc aattttttaa agagcggttc cggcttcaag aaacgatctc ccgctttcaa
    18961 agcactgggc ttccactcgc cggtcgctcc acgcgggtca taacgcaaga cagtatgttc
    19021 acccagatca tcctggattt tttgcacata ttgcgaacca aacaactgat ccgcatgacc
    19081 aaaataagta tgcaatgttt gactggtgaa aggcaagaac ggtgcaaaaa gcaccttgag
    19141 cgaatcaatg cagcgcatgg caacataaat ggcacgggca gcggctggct gatcggtttt
    19201 tatgttcacc cagggcgcgg tttggtcgag atacttgttc acttcggtcg ccaggcgcat
    19261 tatttcagcc agagcggctc gcaaatgcac ctgttccaat tcttttccta ctgtttcaaa
    19321 accaccttcg atggtcatca gaagcgacct gtctaattcc gtcaattcac cggggtcggg
    19381 caccactcct tcccagtatt tataggcaaa agaaagcacg cgatttgcca gattgcccca
    19441 ggtagctacc agttcgtcgt tattgcggtg atagaattct tcccagtccc aatcagcatc
    19501 gcgcgtttcg ggcatgatca cggcaagata atagcggaaa gcatccggat cataacgggt
    19561 caggaaatct tttccccaca ctgcccagtt gcggctgcct gagatttttt tgccttccag
    19621 gttcaaaaat tcattggcag gcacatcgta gggcaacaca aacttttctt ttttctcttc
    19681 acccaacaat tcatcgaatt tttccccaca gccgatcaac tctgctggcc agataatggc
    19741 atgaaacggg atgttgtctt ttccaatgaa atacagcgtt ttcgaatctt gatgtttcca
    19801 ccaatcacgc caggcatctt tcttgccttc cagattactc cattcgatgg atgcagaaag
    19861 ataaccgatc actgcctcga accacacata caggcacttc ccctcccatc cttccaatgg
    19921 aaccggtatg ccccaatcga gatcgcgggt gatcggtctg ccgtgtaatc cttcagcctg
    19981 tatttgaccc aacgactgac ggatcacatt cgggcgccag taagactgcc tctcggtcag
    20041 aaaatcgacc acctctgact gcatttttgc caggtcaagg aaaaaatgtt cggtttcacg
    20101 cagtttgggg gttgatccgt cgatcttgct gcgcggattg atcaatttgt cagtttccag
    20161 cagatttccg catcgatcgc actgatcgct gcgcgcgcct tcaaatccac aaatgtagca
    20221 ggttccttcc acatatcgat caggaagaaa acgcttttgt gcggtggaat accactggga
    20281 ttgtttttct gtataaaggt aaccattctc caacagggtg gtaaaaatgg tttgggaaat
    20341 atgaaaatga ttgcgggtat gggtagaggt gaacaggtcg tatgtgattc ccgcctgttg
    20401 aaacaactcc agaaacccgg tgtgaaagcg ctgatagacc tccagcgaac ttaccttttc
    20461 ggcatccgca cgcactgtga taggcgtacc gtgagaatca cttcctgaaa ccatcaacac
    20521 cttgttacct tttagacgat ggtatctggc aaagatatca gcaggtaaat aggagcccac
    20581 caggtttccc gcatgaattt ccgaattggc atatggccag gcaaccgcaa cgagaacatt
    20641 ttcatgcata gataatctcc aacaaaatga ttttcatttt atcacgaaca ctttcgttca
    20701 cgctgccaat atgaatgctc cgtttccatc actgcccata caagcccttg accgctcaag
    20761 gttttatgaa tacaattaac atataatatg ttctggtgtc ctctccccgg atggaaggac
    20821 acaatggcga aaccggtata caaagtccgg tgctgtcccg caactgtgtg gctctctgag
    20881 ccaagccagg tcgaccgcca gaacgaaaat aaaatcacac actctcgtgg attaggaggt
    20941 ggcatgggtc aaaagaagta atttataatc tgtcaggcgg aggacagatt ttttatttaa
    21001 ttcttcacgc ttgaacgagg atcgatgtga acaaaaaaat agttttcttt ttatcaactt
    21061 tactcattgg gactgtcctt atcggttgca gtgtgcaaac ctctacagcc gaaatggttg
    21121 cggaagtcgc ttcttccagc atatctcttg tggatgggct ggggcgcgag gtaactctgg
    21181 aaagcccggc aaccaacgtc gtctctttat ccccctcgaa cacagagctg ctcttcgctg
    21241 tgggagccgg tgatcaggta gtcggacgcg atacattctc cgattatccc gctgaagtca
    21301 ccaatttgac agatgtgggc ggcggatatg ctgaatataa cctggaactg gtcttggagc
    21361 ttgaacccga cttggtgctg gcagccgaga tcaacacagt cgaactggtg caatcgatcg
    21421 aagatctggg cattccagtt ttctacataa gcaatccgct cacttttgac gacctgtata
    21481 gaaacataga gaacgtggga atactgaccg ggcacacaga taccgcacaa atcgtaatcg
    21541 cagagattca ggagcgggtc gatgcggtgg tctctgccat tgcagaaact gacaccacac
    21601 cacttgtctt ttatgaactg gacgcgaccg atccaaccaa accgtacact cccggaccat
    21661 cttcatacta cagcaccctc atcagcatgg caggcgggaa gaacatcgga gatgccttaa
    21721 cctcatcctg ggcgcagatc agcctggaag aactggttat ccaagatccc gatatcatcc
    21781 tgttgggtga ttcattgtgg ggcgtcaccc cagagagcgt ttcggaaaga gaaggatgga
    21841 atgggctcag cgctgtgcaa gaggggatgg ttctgccctt cgatgacaat cttttggctc
    21901 gccccggtcc gcgtctgatc gatgggcttg aagctctggc tcagacgatc catccggaag
    21961 tattcgatta gtgtctacgc atgcttcctg gaaatggttt ttgttccttt ttgcaggtgt
    22021 agtgattgca gcagctttga gcatcattct gggaagcgtg catattccat tcaaggatat
    22081 atcctcaatt ttcatgggga gggtggaaag cgggtccacc ctccccgcaa catccaacaa
    22141 attcaccagc attctgctcg atctgcgttt gccacgtacc atcctggtat tgttggtggg
    22201 cgccgcgctg gcaaccagcg gcggagccta tcagggatta tttcaaaatc ctttggctga
    22261 tccttacctg atcggagtgg cttcgggcgc cgggcttgga tctattctgg ctatgaccat
    22321 ccaatggcca caggatatgc ttggctactt tgccgtgccg gtcagtgcct tttttggttc
    22381 catcttggca gtctctctgg tatattccct ctccagggtc ggcaaaacca ttcacaatac
    22441 caccctcatt ctggcagggg tggcggtgag ttcgctttcc aacgcgctaa cctcctttct
    22501 gctcatcaac gctggaggag aattgcggcg cgctattgtg tggatgatgg gcggaagcac
    22561 cctgataggg tggaaacccg tcatcgcatc tacccccttt atcctgctcg gcatcggctt
    22621 gctgtacagc acggcttatc aactgaatgt gttgcaattt ggggatgaag aagcccagca
    22681 aatgggcgtt cccgtaagaa aagtgcgcat gctcatcatt cttgccgcca gccttacaac
    22741 cgctgccgct gtagctttcg ccggtacgat cgcctttgtg ggtttggttg tgccgcacat
    22801 tttgcgccgc ctggtcggca acgattaccg tttgctgctc ccgctctcgg cattgggagg
    22861 tgcaattttt ctgtgtgttt ccgacatcct ggcgcgcacg ttgctttccc ctcaagagat
    22921 cccggtaggt gttatcacca tcctgtttgg agctcccttt tttctattca tcttgcgcaa
    22981 tacgaaaagg aaaatgtggt gaggcatgtt acgcgttgaa aacctttcgg tctctttgaa
    23041 caaagagccg atcctcaaaa cggtcagctt tgaattgcac aagggaaaaa tacttggttt
    23101 gatcggtccc aacggtgccg ggaaaaccac cctgatccgc gtaatcaatg gctcgctcaa
    23161 tatggatcaa ggaaaagtta tctttcgaaa gcatgacttg gctgatttgt cagttcagga
    23221 gcgcgcgaaa ttcatcgcaa ctgttcccca aaaccgcacc attccggctg gttttcaagt
    23281 gcgccagatc gttcttatgg gcagaacacc ctatttgaat tggtttggga gatgttcaaa
    23341 agaagatgca aaaatcgctg agaatgcgat gcgccgtacc aataccctgc atctggcgga
    23401 tgttatggca gatgagcttt caggtggaga gcagcagcgc gtcatactgg caagagcctt
    23461 cgcccagaac acacctctga tgttgctgga cgaacccacc acacatctgg atatccgttt
    23521 tcaaatggaa ttcctcgatc tgctcctgga acatgtgaaa gaggaaaaaa agtcggtcat
    23581 catcgctatg catgacctca atcaagccat gcgcttttgc gatgaggtcc tgttgttatc
    23641 tcaaggaaga atctacgcgg atgggaaacc gcaggaagtt ctgacggaga aaaaactttc
    23701 gtccgtctac cagtatccca tcaagatcac acacggaaat ggggatactc cgcgctatat
    23761 ctatccaaag ctctgatcaa gcattgacac aagttctgcc ggcgacgaaa gaataaaatc
    23821 agccgctgct ttacgcagtt cggtgaatgt cccgaaaccg ctcagaacgc cgatggtctt
    23881 catgccggca gcctttccag ccaaaatatc cgccatcgta tccccaacca tcaagcattt
    23941 ttctggtggc acacgcacct gctctgcagc aaagatcaac ggatggggaa aaggtttggt
    24001 gtagtagcag gtttgtgcgg tcacgatcac gctgaaatat cttttcaatg agaaccgctt
    24061 gagaaactgc atcactccgc tctcactgcg tgcgctcaca atacccagtt tataccgtgc
    24121 tgataggatc ttgatcatct tctcgacccc ttcaatcaaa cgcaggtctt tttcaaattc
    24181 ctgccgattg ggtggtcgcc ggcgcgggaa cacccgcata aacaggttat ccaacccaag
    24241 tcgatcgata atatggaagc cggcattaaa gaaagacatg gcaaaagaga ctaaagtgcg
    24301 cgcaaaggca cgtagtttgg gttctttcca cagaaaaagc aaaaaacgca tgtgcttcaa
    24361 gatctgttca acaagctggt cgtcggaatc gcttaacgtt ccatcaatat caaaaaagat
    24421 ggcttctatt tcatcgagca attcctgcat attggtgccc tagtttgcgt tgatataatc
    24481 gatcgcccac aaagcgtctt gataatctcc cctgatctta agcgcgtctc tgaaatagct
    24541 cattgcagca tctttatctc cattttggta cagtgcccaa ccacgccaca acatagcctc
    24601 ttccgatgtg cgtgagatac tcagagccca tgacgtaaga ttcagcaggt cttcggttct
    24661 acctatatga aaataagaga taaacggacc aaattgataa cgcagcatgc gctgcggcca
    24721 accaatattg agggcagcat catacgcctg agccgcctgc agatagttct caaaatagac
    24781 ctgattggta cccacattga accaggcaaa cacgttctgc ggatcttcct ccgtttcctg
    24841 ttgtgcgaca gctaaggctt ttccgcggtt cttgcccaca tcccagtcct cgccgagcac
    24901 agctttgagc gtctgttcct gttcttcacg atacacgatg atatatacgc ggttgaaacc
    24961 ttgccacttt ttgtccaaga tgtcgtaatt gatcgattga ttgggtccta tctcagaatc
    25021 ttgtgcagtg aacgtgcgtg tcgtgtcatt gtagccggtg atcaaaagat aatgccccga
    25081 ccatagatca tcatctggcc aatattgtcg atcggtggtg aaggattctt cgatcatgac
    25141 gggaaaacca gccgccagca gttttttaac gatctcgacc tcaccaccca cgcggaattc
    25201 tgctttcagc cagccagaat aattacgcac atagtaaacc agctcttcca cattcacatt
    25261 acggtcatcg cgagtcggtt tgatcaccgt tgatatatca tactgatctc cactccaccc
    25321 ataatagcgg agatataacg ccagcgtagc tggtccgcag ttgttccaat cctgcttatc
    25381 tttggcttca tcataagccg gtgggttgag gaccaccgtg cttgggatgg gggttggaac
    25441 agcggtggga gttggcgata ccagcggcgt gggtgtgctt gttgccggta gtggtgtact
    25501 tgacggaacg aatggcgtgc tcgcctggct caccaccggc gcaggcagat cctgcacagg
    25561 atttaacaat cgttgtacat acgtgacagc cagatcaatg cgccaactga caattcggtt
    25621 cacccaaggt atttgataca aagcgatgcc caggatcacc atgaaacaaa ccaagtacaa
    25681 gatccttcga cctggtttgc gttttctctt tttatacgga atatactcag ggtaagtcat
    25741 gtgccaattt taccccatcc ttttttatta atacgattga aagagtctga attattgata
    25801 taatcgccgc atgttttatc atatttggac tgagggctgc cagatgaatg tggcagattc
    25861 cacacggctt tcatcagccc tggaacagct tggcttttcc cccacctcac agatcgaaca
    25921 ggcagatgtc attgtattga acacctgcgt tgtcagacag agcgccgaag ataaggcggt
    25981 cggtcggctg tcttctctaa aaaaagctaa agatcgaaaa ccgcagacgg tcatctgcct
    26041 gatgggttgt atggtcggca caggggatta ctctcccctc aaaaaacgtt tcccgtttgt
    26101 ggatgtgttt tctgctccat ccgataccag ccgcctggtc agctttttac tggaaaccga
    26161 gcgggtgagg acgaacgaac atcgttccat ggcgcaagcg atcctggaaa aagaggacga
    26221 tttcatccta ccggttgaaa tgcgcggaaa cacggtagcc gcgttcatcc ccattgtact
    26281 gggttgttcc catgcatgca gttactgtgt gatcccgctg cgccgcggga aggaaatcag
    26341 ccgcccgcca gcggatattc tggcagatgt gcgctcgctc gccaaacagg gtatcaaaga
    26401 ggtcacactg cttgggcaga tcgtggaccg ctatgggcgt gagaatcccg cttatccaac
    26461 actgggtgaa ttgataagcc agatccatca gaccgaaggg atcgagagaa ttcgtttctt
    26521 aacctctcac cccaattgga tgacggatga actgcttgac gcattgagcg ggctgcccaa
    26581 agcgatgcct catttcgaac ttcccatcca agccggggat gatgaagtgc tgcggcagat
    26641 gcgcagggga tatacggtcg aaaagtacat aactatcgtg aaaaaaatac gctcacgttt
    26701 tcctttttct tccattgcaa cggatctgat cgttggtttt ccgggtgaga gcgccgagca
    26761 gttcgagcat tctttacaaa tgttggctga agtgagaccc gacgtgactc atgtggcgcg
    26821 ctattccccc cgaccaggta cctactcggc aatgaaaatg caagacgatg tatctgccga
    26881 agaaaaatgg gagagattcc gcgcggttga agccatgcaa gaaaagatca gcggagagat
    26941 caataaacag tatctaggga aaaaggtctc agtgctattt gaagggcaaa agaaagaacg
    27001 ctggttcggt cgcacgccca ccaataaact ggtctttgtg aattctgccc aaccattgtt
    27061 gggttcagta caggatgttc ggatcacctg gagcggtccc tggtcgatga tcggggagat
    27121 cgaggcaaaa gaattggcat gaaaaagatt gtaagatttc tcttgatagg aatagggatt
    27181 gttgtcgtgg ttgtggtgat ggtcttttat gagaccgtct ttcacccaca acgttcttct
    27241 aaagtgctcc aatggctgcg agctcctgaa gaacacgccg agtggtccgt acaagccagg
    27301 acacgctgtg gagaagctcc attcatgttg cctaccagcg gattgatcgg atacctttgg
    27361 gatgattcgt tccgcatcgg tcaccaccac caggggatcg acatctttgg aggtaccgca
    27421 gccgggatca caccggtcta cgctgcctat gacggctacc tcacccgttt gtctgattgg
    27481 aaatcttcgg tcatcatgcg cattccggat gacccgctgc aaccagggcg ccagatttgg
    27541 acttattaca cacacatggc agataaaaat ggcgaatcct atatcgtggc agattttcct
    27601 ccgggaacag aagaagtttt cgtgaaggca ggcactctgc tcggatatca gggaaattac
    27661 tccggttcag ccaataatcc aaccggggtg cacttgcatt tttcaatcgt tctatcgggc
    27721 gctgatggaa atttcaagaa tgaattgaag atcgagaaca ccatcgatcc ttcgccgtac
    27781 ttcggcttac ctctcaatgg aaaagaaaac aagggcgagg ttccggtatg cagcagcaat
    27841 ccataaccgg tttctccatc ttataattca caccagctat gttacagtac cgtgatttga
    27901 aaaatgtctt gcgcgaactt ggggttaaga gaagtgaacc gaccattgca cacatctcag
    27961 cagcgttgat tcctcatgtg aaaggcggag cccagaccgt acttggcgct gttctggcga
    28021 gcattgataa tatcctcacg cccagcttca cgtacaaaac gatgcttgtt cccgaagtgg
    28081 gacccgacga taacctgctg gaatatggca gcggcaggct tgaaaacctg aatgcttcaa
    28141 tttacacgcc agatctgcct gctgattttg aagactgcgc catcagtgaa gtcttccggc
    28201 attatcctga tgtcaaacgt tcaagccacc ccatgctttc cttcgtaggg ttagggttgg
    28261 atgccgcctt aaatgcgcaa tcaaccacca acccctacgc gcatatccaa tatctgcggg
    28321 gaaaaaacgc cagcgtcttg ctggcaggca agggccctgc tgcgctgttc gctttgcatt
    28381 tatgcgaaat ggaaagtgga agaaagcagt ttgtacgttg ggcgatgagc gaggaagggg
    28441 taaaagaatg cctgcactac cctggctgtt cgcaagggtt ccataagatc attttccatc
    28501 ttaagggtaa agtcaaacaa acgctgctca acgaccagca ttggtatgct ttttctcttg
    28561 acgcgctgat tgaagtcgca acccgcctgc tgcaggaaga tccctatgcg ttattgtgca
    28621 atgatcttca ctgtgtctca tgcaatttgg taagaaagga tatccgacaa aaaggaatgt
    28681 aagaacgacc gtgctgttta tacaaccgtt tggcatataa tacaaaaaga tagaacgcat
    28741 tacagttttt ggagactttg catgaaagag atcaatgaaa atatcgttat cgatactacc
    28801 ctaagcggtg tcaccgtggg cgttatcaag ttccccaagg gagtgatttt cattgattcg
    28861 ccgcttactc caaaagatgt tcaatcatgg aggaccacag ccatgaagaa cgacagcgga
    28921 ccttcacgcc ttcatatagt tttggatgag cattatgacc gcacggcatg cgtaattccc
    28981 atcaaaagtc cggtccttgc acatgaaaag actagcaaag ccatccaaag ccgccccaca
    29041 tttttccgat tccctacttc tgatacaggc agtgaatggg aattgtatcc cgaggtcggt
    29101 ccgatgcagt ggatcaatcc tgaaatcaca ttcacgcaat ccatgaaact ggaattggat
    29161 gatgatgtgg tcgaacttga ttatcacccc gggccttcta gaggagccat ttgggttggc
    29221 atgcctggtc caaagattat cttcatcgga gacactgcgg ttgtggaaca gccgcctttc
    29281 ctggaaaacg ctgacattga acaatggttg gaagcattgg cacttcttaa atctgatcaa
    29341 tacaaagatt acaccatcat cagcggacgc tccggtgtga ttgagcgaaa cgctatccag
    29401 gaacaaatga agtttctctc cacggtacaa aaacgattag aaaaattctc tggcaaagcg
    29461 atcgattctg cccaattaga aaaaatagcg accagccttt tagaggaatt tcctgccaag
    29521 acgaagaaaa tccaggaaca tttcaaagcc cgcttgaaat gggggatcag ccagtattac
    29581 gggcgcagtg tgcgctaaac tgccaatcaa tataaacgag gatatgcatg tctgaacaaa
    29641 catatttacc gctggttgaa ttcacacgcg gtgcaatcat tgaatctatt cattgtgggg
    29701 tagtggtggt cagcgatcat gcagggaaca tcctctttaa aatcggagat gagaacctga
    29761 taacctattt gcgctccact gccaaacccc tgcaagtatt agccctgctc gaacatccag
    29821 aggctgctca ttatcatttt gaggacgatg agatcgccat catgtgcgct tcccactccg
    29881 gcaccgatga gcatgttgcg gttctaaaaa agctccagca gaaggttggc attgatgaaa
    29941 atgatctgca gtgcggcacc catcttccgt tcgaccggat tacagccaac caggtggttc
    30001 gaggcgaaac acggttatcg accctgcagc acaattgttc aggcaaacac agcggcatgc
    30061 tggcgctggc gaaattgctg gctgaaccca aggaatctta cctttctcca gaccgaccca
    30121 cccagcaatt gatcttgcag acctgtggag aaatgctaga tattccgttc tcgcgcttcg
    30181 aaatgggcat agatggctgt tctgcgccag ttttcgccgt gccaatgcgc aacgcagccc
    30241 aggcgtatgc gcgattatgc caacccgatc acctgacacc ccggcgaacg catgcctgcc
    30301 gctgtgttac gcgcgccatg atggctcatc catttctggt agccggtcct gaacgctttg
    30361 acacggcagt gatgcagtca ctgcccggaa aactgatcgc gaaaaccggg gcagaaggtt
    30421 tttttggaat cggaatcatg cctgactcca tcaaaccggg cagcccgggc ataggcatca
    30481 tggtcaaaat ttgcgatggc gatctgaaag agcgagcccg tccggtggtc tgcatgacca
    30541 ttctccgcca cctgggtctg cttacgcccg atgtagagac cctgttgaaa acgtacgaca
    30601 ggggaaacat atccaactgg cgcgatatcg cggttggaga agttcgccct tcgacagaac
    30661 tgctggaggc tttgcaatcg tggaaaatat aaccaccctg ccgaacgatt tgaaggaacg
    30721 ctttgctacc gaccaaagga attcacagtg gttgtaccaa cagcttttag cgacctatgg
    30781 ggtgcctgta tggcgcaaac ctatgccggc tattgatgag ctggtctcaa ccattctctc
    30841 tcagaacacc aacgaccaca atcgtgatct ggctttcgat cgccttcgcc atcgctttcc
    30901 cacctgggac gcgatttgcg acgctgatat agaagagatc gtggatgcca ttcgaccggc
    30961 gggtctgggg aaccaaaagg gcaggaacat ccaggctgcc ctgcagcaaa tctaccagga
    31021 gcaccgcaga ttggatcttg agtttctcaa agaatgggag cctgatgaca ttcggaatta
    31081 cctgctgcag ttcaagggag tcgggttcaa aaccgctgct atcgtgatgc aattttcgtt
    31141 agaaattcca gccttccctg tagataccca catctatcgc gtgaccgggc gtttcgggat
    31201 ccgcccaaag tctatgaatg ttgaagactg ccatcagtac atggaaagac ttttcaaacc
    31261 tgaacaatat aaagctggtc acctcaacat catccgcctg ggaagggaga tctgccaggc
    31321 acgcaaaccg ctgtgtgaca ggtgcccgct tcgttcgcgc tgcgcttatt ttagcgaaca
    31381 acaaacgaaa taatgatata gttacccaac ttagctcgtt caattcgcag aatcacttag
    31441 gagaaagatg aaatgaaagt aaccttatta ctagagaaca actgtctctt tgccacatat
    31501 ttgaatgccg aacatggttt ttcagcctgg atcgaagacg aagatgtgaa agtcttattc
    31561 gatttcggat tctcagataa ttttattaga aatgctgaag ccctcgggat cgacctgcgc
    31621 acggcagatt atgtcattct ctcacatggt catcgtgacc atagcggtgg gttgaaatat
    31681 ctcatgaaat actaccgcga aaaagccatg gcgcgtaagc ccatcatgct gatgaccatg
    31741 gaagatatct tctttaaaaa atacaatttc aaaaagggac atttttcagg attcgacgtc
    31801 agtcgggaat ttttggatca atatttcgat gtgcgtatca cctccgaacc tgtatggttg
    31861 accaagaacc tggtttacat gggtgtcaca gaattgacca acgattttga gcataaagtg
    31921 ccccagatac ccaaaaagtt agtaaacggg gaatgggtgg atgacgtggt ggacgaagat
    31981 acccagcttg catatcgaca taaaaacgga aaagagacca gtgtcattgc tgcctgcgct
    32041 cattacggca tatgcaacat catggaatat gccaaaaagc taaccggggc accgcagatc
    32101 aacacttacc tgggtggctc gcacttgcgc attgatgagg ttccgcaatc acagttagat
    32161 aaaaccgtgg aatacgtgaa aaagcaaaag atcaaggatt tttacatttg tcatgatacc
    32221 gacctctatt gcaaacaggc gttgtggaac gccacaccca gcaaagaagc cggcacggga
    32281 ttggtcatcg agcttgaata acggatgatc ggtacattca caagcgaaaa agccagttat
    32341 cgttcaactg gcttttttta ttcaggggca tgaacgcgca ccgcccaata tccagaacct
    32401 ggctggttgc catttaccag tcgattgaaa atctcagaat ccaccgtttc ccagtacggc
    32461 gtgaacaaag gaacaatctc aatccaggtc tgccctggtt ttaatgggaa agggttgcct
    32521 tccgcatcca caaaacggat agggcgcagt ttgccggtgg tcttttcata ctcctcgctg
    32581 gcagtcgtcc aataaatatc gtactttttc ccatctctga agagcaaagc ctttcctcgt
    32641 ttaatgtaca acagggaaat atcgatcatg gtgatgttcg tcacctcatg gtctgcgaaa
    32701 aggacaatga cattggctgc cttcaggggt tcacctgtca aacggtcagc agcttgaatg
    32761 aaagtggtgg catccgcatt atcctgccag cgcagaaatg ctccgcttgt ttcatcatag
    32821 cgccaaaaga tctgattcag gtaagcgtag aatagccaga gcgaacgggc ggttttgccc
    32881 ccctcaggca aagtagaatc gaaatacata ccgctcaatg ccgcctcccc aattccttgg
    32941 ggtttgatct tgccaatgcc tccaattggg tcactctcat catggcacta ttgggatcag
    33001 aaagatcatt tccaaagaaa ttggtgtaag cactgatctg ctgagaaacg tttggagatg
    33061 ctgaagccat gaccagatac ccgctgaaca ggctacgaat cgattcatag ggcagccgcc
    33121 ccgagcggat aggacccacc agcggttctt cgacagtggt ggtagtttca tcctccggat
    33181 acgcatctgc aaccctctcc ggaaaatctc cgtagaagac tcccaaaaag cgggtcatgc
    33241 cttccccgat ccatatctca aagacaatcg ggcagtacga aaggcctgcc tgcggacgcg
    33301 cggtgggcgg aaaattgctg atggatacca gaaacggggg atttttcagg ttctcagggt
    33361 gtttgacggg tagcccattc agcggattta cattgtgcgg gaagctgagc gggccaagag
    33421 tttgtggggt tgatagcagt gttggcacag gggaagaaga aggggatgct ggagcagttg
    33481 aatccccagc agatttttca agcgcgccgg tggaagcaag gggaattgat ggcgtcgaga
    33541 aactacagct ttgcaataac aacgaaaata aaaacagcat agtgataaga tggctggctt
    33601 tcatttttca actcctcaat ccattgcaaa ttaattccat cattgacaaa atgccgaatt
    33661 ctgcttaaac tatggtatag tggtatgacc aatataccac aaaagcaaat gaaatggaga
    33721 tgaagtatga agatcaaaac tgtatttggc ataatcctga tcgtgatgct gctcgcagcg
    33781 tgcagggtca cgccaaacac aaccgcaacg caaaactccg caccgttgac cagtgatctg
    33841 cagcccgaac caaccgggtc ggtgctatca gatgatctga ttttgcgcga tgaattcgat
    33901 gatgacatca ataaaaattg gggaatgaaa atcgtttcag gcttagaaaa ccagctcatc
    33961 tggtcacaga ccaacggaaa attcaggctt gagatccagc cacccaacga taccaactac
    34021 gctttcttcc gcaaaaatac ttcgtaccgc gatgtgatcg tgcaggctga agtgcagaat
    34081 tacgggcagc tcgacaatgc tttttcattg atgtgtcgcg ccaccgaagc gggctggtat
    34141 gagttgcgca tcagcagcca ggggtattat gaattgctgc gctacgacca atacctggcg
    34201 gacgaaggca agaacgcata cacgaacttc gtgcaaacgc gggtgggcag tacgttcatc
    34261 aagggcggtg aagagaccaa tgtctttgct ctttcctgcc agggcaccca gctcaaggta
    34321 tttatcaacg gcgaacaggt ttattacgat aaacgcccgc tcaccattga agataccacc
    34381 tattccgagg gcaccatcgg atttggcgcg ttgagttatg ggaaagaagt ggatgtgacc
    34441 ttcaattata tcgaaacgct aaagccttag ccctcctggc ttagcggtac cccttaacga
    34501 aggaggtctt tcgacctcct tcaatattta atctggtcat cgttttcaac cttcgttata
    34561 atcacaggta tgctcaactt gtggggaaac ccatgccatt aaaccacctt gatttgcaga
    34621 atcagattca acgtttttcc agagacgaac tgttggctca tgaaaatgcg gaaaagcaac
    34681 tggttcaggc atcaaccgtg ctagagcatg tttcccacaa catagaagct atgcgcaaag
    34741 ttggtgaggt ggctacaacg cgtactgcga ttcccactac cgaactccca aatcaagctt
    34801 ttcgtaatgc tcaatggaat gatgtttaca acctggtctg tgccgatggt tcccagatca
    34861 tgcccgaccc gcacagctcg ccgatctttg ccctgctgaa catcggcatc atccgcattc
    34921 cagcggaaaa atcgaaagca attcaccctg tcatcatcac cgacctgtac cagcgcgatc
    34981 gcctgtatat caaaaaccaa ttgatcggcg aagatatcat agccctcgac cgcgatgtgc
    35041 ttgaattgcg gtatctttac gaaaactgtc aggagctaaa aggaacaacc atcgctttgc
    35101 gtgacgggtt gttggaactg tatcacgagc cgcgcagtga caatgcgttt caggaacgtt
    35161 tgcaaaccta ctatgcttat ctgcaatcgt tccgatctgc cgaactcagc atcgccgggt
    35221 atgtggacaa accgcgctct caaatggtgg tggagctctt gcgtttgtgc gtggaaatgg
    35281 aagaaaaagc ccatagcgaa aacacctatt tcccagatat caatgatcgg ctgctgttcg
    35341 aacagaggtt gaagaacggt gaacgatctg ccattttcga aaattttctg cccaagaatg
    35401 atctccacgg aactttagct ccgtttccct tttatttctt ttatctgaac gtcagtcaaa
    35461 atgaaaaacc ctggatcgtg cgggtggaga ttccggaatg ggtggcatcg gataaagcca
    35521 gagtggaggt tctgcatagc gcattattgg aacaatgcgc tatcatggga accaaaccct
    35581 atccatattg cctgcaccgc gcccatgaaa cggcactggt tcgtcaggca gaaaaagagg
    35641 aactagccag gcaaattcaa gcaatgcaac tcaaagaagg tcttcctctg gatgagaaat
    35701 cgtataaaca gagtgccaaa gaccatacat ctcgaagaat agtggagcgc tagcaatgaa
    35761 cgagaaaagg atcggttatg tcttgaatgc ggatacattg ggttgtaccg ctggctgtcg
    35821 catagatcaa accaccaacc ctggttttgg ggaaatgatc acgatcccgg ctcaagatca
    35881 acgaatcatt tatgggatca tagccaatgt gcacattctt gacgatgggc tgatcaggca
    35941 gcttgccaca acccagatca ttgacgaaca cgtcatccag gacaaccgca ataaccgcgt
    36001 cattccgttg gagtttagtg tcatttttct tggcttcaat caagatcaac aaatctatta
    36061 tctgctgccg cccaatcccc cactctccct ggaatctgtt tacgcctgct ctgatgagaa
    36121 gagcattcaa ttcacctcgc ttggcagcgg ggggtacctg cgttaccttg tggctgataa
    36181 gaccacgctc aattttgatc tggtggcagc gcattttcgc catctgcatc agatactacc
    36241 acagcagaat tggaagccct ggatggaaaa tgccacccgc actctcatcc atttataccg
    36301 caatgattac gacacactga acgccctgct acacacttta tccgacacat caatatttgc
    36361 tgattaataa cacaataaag gaaatggaac caatggtcac acctacaccc tcactgcccg
    36421 gcaggcaaat tggaacggtt atcggaggca gcctctccga gaacctggtc gcccgcctgc
    36481 agatttctcc gcaagaaata caagaaggca gttttgtaac catcaagagt ggcaattggc
    36541 agttctacgg tctcatcacc gacctgcgtc tgggagcgac cgacccgcgt ttttctgatg
    36601 aacagatcga gacttacttt ccaaagtcaa tctcacaagc tttgcggaat gaagtgttgt
    36661 attctaacgc catcatcctg ccggtcttga tgctagaaaa tggacctgac tttgatcagg
    36721 agaatattaa agcgcatctg gcatggaaag cgcaaaaaga ggaggcgggg gatttgatgc
    36781 tgcaccccat ccagatcaaa accatccccg cccaccaatc ccccgtgcga ttagccagcg
    36841 ctgtggatat tgccaccgtg ttcgggaacg agaatgaggg gaataatttc aagatcggca
    36901 ccacccgtga gcagaacttt ccggtgtgta tcgacctctc tcatttcatt caacgctccg
    36961 ctggtatttt tggcgcaacc ggtacgggaa agagcttcct tgcccgcatt ctgttagccg
    37021 gattgatcaa ccagaacaag gcttcgctgc tcgtttttga catgcacgat gaatatggtt
    37081 ttgacgacac tgcttccgac accggggaaa aggtggtcgg cttgaacaca tattttggat
    37141 ccaaagtgca ggttgtcggt ctggcgggcg ggcgaaacat ccgcggaaat tcccccgctt
    37201 ttcaggtgca gattgcagaa cgcgatatcg aaccaaccga tatcgaaatg ctggcagggg
    37261 aattaaatct caaagaaact actgccacaa cgctggcagc gttggtatct tcatttggaa
    37321 aggaatggtt cgccgaattc aaagcgatga agtcagggag catggtcaca gatgaagatg
    37381 gcaagaagat cgcggctcct gatagcgttg ctgcctgggc aaatcgtaat gggattaatg
    37441 ttatggctgc cgaaagtttg cacagcaaac tgggcaggtt gttcaccgcg aaatacatct
    37501 ccgccaatcc agccactaac gggttacaac agatcgtaca attgcttaag accggcaaac
    37561 acatcatcct ttctttcagc ggctacgata ccgatctgga ttatcttttc atttcaaatc
    37621 tcatcacgcg caagatccgt gaggaatggg aaaaactgac caacctgtat cgcagtgaag
    37681 tgaataaagc gagtgaacct cgccaactgg tgatcgtggt ggaagaggcg cacaaattgc
    37741 tcaatcgcga gaatgccacc caaaccgcct tcagtaccat cgcacgcgaa atgcgcaaat
    37801 attacgtgac gctgctgatc atcgatcaac gcccttcgca gatctatgac gaggtgctct
    37861 cacagcttgg cacgcgcatt tgtggttacc ttaccgatga tgaggatatc cgtgcggtgc
    37921 tttcaggggc cgccggcaaa gaatcgttgc gcaacatgct cgctcatctg cagcccaagg
    37981 aagaggtgtt gatcgccggg tggggggtgc ccatgccaat tcctgtacgt tctcgccgtc
    38041 tggatgccgc gttctgggaa gatctgggaa acagaaaatc gaacgtcgtt attgcgcaag
    38101 atctcgcaga acaaaagaag aaactgggat tctagcttca gtaataaaca gctcgcatga
    38161 tcaacgctgt acagatgccc agggtaatgc cagccagggc ttcaaacggg gtatgcccga
    38221 gcacctcctg caattttttg taagaaaaat ccccgcttct taatatctcc tcaatgatcc
    38281 tgttgatcca ctccgcatgg aaaccggctt gtctccgaat actgaccgcg tcgtacacta
    38341 caatggttgc caaagccacc gccagggcaa acagcgatga gtcgaaccca tcatgaaacc
    38401 caatagcaag cgcagtcgct gtggaaatgg cagaatggga actcggcatt ccccccggat
    38461 tgatgagcac catccaattc cactcttttg tgttcaagta atgaagcggc aatttcaaga
    38521 gctgcgccga taaccaggca gcgagtgctg aagccaaaac ataattggag accaattgcc
    38581 ttaaaaactg agtgaaatca gtccacattt tcggttggat ttcctgtagc tggttttatt
    38641 tctcgaatgc gaagttcagc tttttccagc atctcctggc agtgagccag cagttcttta
    38701 ccgaattcaa agcgttccaa catcctatcc agttccagcg agccattctc aagatctgct
    38761 aatacctttt ccagttcctg catggcttct tcatacttca ttttttttat gtctttttgc
    38821 gctaccatac cgtcgttcat tcctgttcat attttgagac cacttgcgca gccagtctgc
    38881 catccttcag gcgtatagct attttctcat ctttgtcagt tttggcaaca ctgtccacca
    38941 gtttgttttc ctggtcgaac accagagcat atccacgctg taaaactgct tgcggatcga
    39001 gcgcacgaaa gcgccctctc atgctatcca aactggcgtg cagcaaatcc atacggttgt
    39061 gcatcaaaag gtgcaaccga tgagccaatt catcgctttg cagcatagcc tgccggatca
    39121 gcctctcagg cgaaagatgc tgcaagcgga attgttggtc cttccaatcg attcgtctgc
    39181 tttcaatatt ttctatacac aactgcataa aagacacctg gatttcttgc agggatattc
    39241 tcaattcttc gatatccggc acagccaata ccgcagctcc ggttggggtg ggggcacgca
    39301 catcagcggc aaagtcaacc agtgtgaaat cggtttcatg ccccaccccg ctgatgatgg
    39361 ggatatcgct tgccgctacc gcacgcacga ctgcttcttc attgaacgac cacagatcct
    39421 caagcgaacc ccctcctcgc gccaggatga tcacatctgg tttttgggga gccctctcca
    39481 gggcgtgaag cgcccgcacg atgttttggg ctgctccttc cccctgcacc agggttggac
    39541 tgaacagcac ttcagccaag gggaatcgct tacggagcgt gttcaagata tcttgcagag
    39601 cggcgccgga tggtgaagtg accactccga tcaagtgagg aaaagccggg ggtgttcttt
    39661 tctgctgagg atcaaacaaa ccctcttctt ccaattttct ttttaaccgc aaaaattcct
    39721 gatagagctt tccttctccc tgcgtgcgta tggaatccac atagaactga tattgtcctc
    39781 ccttttcata cacaccaaat ttgccgtgcg tttccaccgc catgccatct tggaggtgaa
    39841 aaccgagata ttgcgccatc catttccaga tcacgcaacg tagagaagcg gattcatcct
    39901 tcagggtgaa gtagacatga cctgatgaag ggagcgaaac gttcgagatc tcaccctgca
    39961 cccagatatc ctggagatcg gggttctctt ctaacagaac ccgtatataa ccggtcactt
    40021 cggagactga gtaaatctgg cgctgaaaaa gagtattgtc gatcatgcaa atatcagtat
    40081 aatctgtttt ccgcggaaac agcgcgtata ataagaatct gaatatcgga aagaaacagg
    40141 tatggaatta tggaacatgt ttgggcacca tggcgcatga aatacattca ggaaacctcc
    40201 gaaaaagggt gttttttctg taaggggata gccctgcaag atggtgccga taatttgatc
    40261 gtcaagcgtg gcaaatacgc cttcgtgatg ctcaaccgtt atccctatac cagcgggcac
    40321 cttatggtcg tcccctacgc acatgaagcg tctttagaaa aattgccagt ggaaaccaag
    40381 actgagatca tggaactgat cgcccaagcc attcaggttc tcagcaaaga atatggcgca
    40441 gaagggttca atgtgggtgc caatctgggt ctgacagccg gagccggcgt ggctgaccat
    40501 ttacattttc atgttgttcc acggtggggt ggcgatacca actttatgac caccgttgga
    40561 gacgcgcgcg ttctgccaga gcatttgatt gacacacaaa aacgaattca agaccactgg
    40621 gtaaaaggaa cggattgatc cctggatgag cacagtttat gtccgtgaaa agacttcgcg
    40681 cgatgctgtc tggatccagg cactcatgct ggaaagttgg gggagtgaga tggtggtact
    40741 gcaagggcgc ctttaccgtc cggcagaatt gcccgccttc gtcgcagaac aaaataaaga
    40801 gcgctgtggg cttgtaacct atatttttga tgacaattca tgcgagatca tcaccttgaa
    40861 cagtttacaa cccggcttgg gaatcggaac acaattgctg gatgcagtaa aacaaagcgc
    40921 tctcgaaaac ggttgcaacg aaatcaggtt gttcacaacc aacgataaca tcaatgcttt
    40981 acgtttttac caaaaatacg gtttttcttt ggaagaattg atcaaaaacg gagtcgccct
    41041 cgaccgcaaa ttgaaaccag agattccatt gaaagcggaa aacggcattc cgatcaggga
    41101 ttatcttcgt ctcaaaatgg gattgaatcc atgaaaatca tcttgaagat cgcagcgctg
    41161 gcagccaggt ttttgccagc ccgtgcaaaa gcccagctat atcgaaatcc caaactggca
    41221 agcaagatca ggaacacgct caacgcctcc gctccgcgtg gattgacgga aacatgggta
    41281 gccggaggat tgatccaggg gctcttgctc aaccttgatc tgcagaccga gaaagattat
    41341 tggctgggca cctatgagat cgaacttcaa accgcaatca agaaatactg ccgccccggc
    41401 atggtgatct atgatgtcgg agcaaatatt ggttacgtga gtttgatttt cgcgaagcag
    41461 gtgggggaga aagggaaagt gatcgctttt gaacctcttc ctgaaaattt gcagcgtctg
    41521 caaaagaaca tcacgctcaa tggtctgggc tctcgtatcg cgtgtgttca ggctgctgtg
    41581 gtggacaaac ctggttccat cacatttctc gtgcacagtt caggcgccat gggcaaagcc
    41641 caggggtcat tgggacgaga ggagcaatat gtggcgcaga taactgtccc ggcggtagcg
    41701 cttgatgatc tcattttttt acagaactat ccccaacctg acctggtcaa aatggacatc
    41761 gaaggtgggg aagtgcttgc gatacaaggc atgcagaaaa ccttgaccga agtgcagccc
    41821 atcttgctga ttgaaattca cagccgttct gcgcttgatt ttctttggac tgaattgcag
    41881 aactgtggtt accaattgtt caagatggaa agagattacc ctcacatcac ccatccctcc
    41941 acgttgggag aaaaaacgta cgcggttgcg attccaaaag aaaaagccgg ttgaaattct
    42001 caaccggctt ttttggagct tacgagaaac ttatggttct tccgggatta ccagcatcat
    42061 caccaggtat acccaaaaag tcagggtgcc aaggaacaaa ccaagcacaa ataacaagcg
    42121 cacaatcgtc ggatccacgc caatgtattc acccagcccg ccgcatacac ctgcgatcat
    42181 tcttttatca cgcgagcgat acaattttcg actttcatca gccatggctg ctctccttta
    42241 tcaatttcat attcccttca tatacgataa acgaaagaga atggtttcta ctttttcgat
    42301 ggattcgtct tttttctggc tttttcttct cttagtaatg caaggaaagg ttccacaacc
    42361 tttggatcga aatggccccc cgattgctct ttgatatatt cgatcacttt tttctcagcc
    42421 caggctttgc ggtacggtcg atctgacgaa agggcgtccc atacatcaac gatggcgaat
    42481 attctggcag ataagggaat ctcctctcct ttcaacccct gcggatagcc gctgccatcc
    42541 catttttcat ggtggcaata gggaatttcc agtgctggtt ccagaaaatg gatagaggag
    42601 agcatttcat aagcaatgcg agggtgctta cgcatggttt cccattcctc ttcagtgaga
    42661 ggtcccgctt tctgtaaaat gctatctgga atagcaattt tgccgatatc gtgcagcaat
    42721 gaaccgcgcc ggatatgaag cagagcatct ccgcgcacac ccattttttc tgccaggcgc
    42781 agggtcattt ccagaactcg ctggctatgc ccttc
//
LOCUS       DAOSDS010000007        41872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_523_length_41872_cov_5.468733, whole genome shotgun sequence.
ACCESSION   DAOSDS010000007 DAOSDS010000000
VERSION     DAOSDS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 41872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41872
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_523_length_41872_cov_5.468733"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1391
                     /locus_tag="PLL88_01710"
     CDS             165..1391
                     /locus_tag="PLL88_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOJ00308.1"
                     /translation="MSEYINNSSQREEQLKEIIKQLHAGRAFADLKDTFQDLLKDASA
                     QEIVQIEQALIAEGLPVEQIQLLCDVHVALFKESLDTQLPPEMQPGHPIHTFRSENEL
                     VELVLHSMDEKVAKLTLSDDQELRNQLSNDVEKLKQMDRHYLRKENLLFPILERYGFT
                     GPSSVMWGIHDEIRAGWNELGKLITDAPVDQFSEKYQQLQHKIREMIYKEDRILFPSA
                     LERLTDQEWGTVYAGEAELGFAYVTRGNDWKPQIQANPVKSEVDYRVNKDEKEMDMVE
                     LTLNVGKLTAKQVNLLLQNLPIDVTFVDENDQVAFYSQSRERIFARTPAVIGRKVQMC
                     HPPASVDKVQKILDDFRSGKRSEAAFWIQMKGKFIHIRYFAVRDEENTYRGTIEVTQD
                     ITEIHQLEGEKRLLDD"
     gene            complement(1431..1820)
                     /locus_tag="PLL88_01715"
     CDS             complement(1431..1820)
                     /locus_tag="PLL88_01715"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOJ00309.1"
                     /translation="MRASSITNAILKVLEDQEDHLTAKEVYEKVKGTLSAVDPSTVYR
                     SLERMVHQGQVSVSDMGGPAVVYELVAKEPHHHLVCEKCGKVLTFQRGEMHDFLNRVH
                     AKSGYQITTNHLVLFGICPDCQKKNDP"
     gene            1889..2839
                     /locus_tag="PLL88_01720"
     CDS             1889..2839
                     /locus_tag="PLL88_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HOJ00310.1"
                     /translation="MFVKQQLMHIPDGFLSFILSLILWILSGIVIFLAIRHYAKEKEN
                     QKLPLMGILAAVIFAGQMLNFTVVGGTSGHLLGAALATILVGPWEAVLVITSVVAVQA
                     IVFQDGGLLALGANIFNMAVIGVLVSYAVYSLLSRIIKKGKWSVGIAGFLAGWLSIFV
                     AALAAALELAVSGTSPANIAVPAMAGIHALIGIGEGLITAAALLFVAATKKELLEGSQ
                     KPKAVDASILIGGILIALVLAVLSPLASAYPDGLERVAEDLGFLSKRQGPLYTVIPDY
                     TFPGIHDERLATIVAGVLGVVLVASVALFTHRGKKNSAQK"
     gene            2852..3643
                     /gene="cbiQ"
                     /locus_tag="PLL88_01725"
     CDS             2852..3643
                     /gene="cbiQ"
                     /locus_tag="PLL88_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HOJ00311.1"
                     /translation="MYHPGSDFLFSVDTRVKWVGVIMIAFIVLTIPLFTWAVYILLFA
                     ILVALSISSRLPFKQILKRTLLVEVPLLFILIPLPFINPSTATTRISLLGLSIDFSLP
                     ELLRVGVLLLRSWLIIFSMVVLTVTTPADELLASLSSLGVPAVLVTIILLMWRYLVLF
                     VSKAKKLSAARELRSALPQDGNYKNWHRLRLSVQSTGSMLGGLFLQAYEQSDRVYQAM
                     QLRGFDGTLRSRKPEPLRTSQWVQIFLLLVFGLSLILGAYGIYGK"
     gene            3633..4358
                     /locus_tag="PLL88_01730"
     CDS             3633..4358
                     /locus_tag="PLL88_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00312.1"
                     /translation="MESERTPILQVRDLRFAYPDMPETLNAISFSVARGEKVAIAGPN
                     GAGKTTLLLNLTGLLKGEGEVYVEGLRLEKGNFSAIRRILGLVFQSPDDQLFSTRVFD
                     DVAYGLVYQGMEKAEIEERTRKALADVGMAAYESAVPHHLSLGQKKRVAIASVLCMQP
                     EIILFDEPTMGLDARAKKQFLEIAASLTQTMLVATHDLRLAVALCERLLILDEGRLVF
                     DGEMKKALRDREFLERHGLDWDV"
     gene            complement(4441..5184)
                     /locus_tag="PLL88_01735"
     CDS             complement(4441..5184)
                     /locus_tag="PLL88_01735"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5050..5052),aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOJ00313.1"
                     /translation="MKLKVGQHAPDFTLPSHLEKDLTLHDLRGKTVVMAFFPLAWTPI
                     USGQIPSYEVLLPKFAGLNVQVLGISVDHVPCLKAWADSLGGITYPLLSDFWPHGKVA
                     KKYGVLRSEGYTERAIFLLDEEGIIRYIDIHDIDDQPSNEVLLNEIKKITPEKFFDDE
                     ILPQDESLPHGGVVMYCTQWCPDCRRARIWLKENNIPYTEVDITTNFTAARQVRSWGN
                     GSQITPTFDIDGTIILDFDEEELSEILLK"
     gene            5261..6007
                     /gene="gpmA"
                     /locus_tag="PLL88_01740"
     CDS             5261..6007
                     /gene="gpmA"
                     /locus_tag="PLL88_01740"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOJ00314.1"
                     /translation="MYKVVLLRHGESEWNKTNLFTGWTDVDLSEKGLQEAKQAAELLK
                     KEGYTFDIAYTSVLKRAIRTLWIVLDGMDLMWIPVIRSWRLNERMYGALQGLNKAQTA
                     EKYGEEQVHLWRRSYNVPPPALEKSDERYPGHDRRYAELAEKDIPTTECLKDTVERFL
                     PFWENTIAPMIKTGKRVIIAAHGNSLRALVKYLDNISDEEIPELNIPTGVPLIYELDD
                     DLKPIAHYYLGDQTAVENAIHAVANQAKKE"
     gene            6112..6765
                     /locus_tag="PLL88_01745"
     CDS             6112..6765
                     /locus_tag="PLL88_01745"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOJ00315.1"
                     /translation="MITENVQRVLKCVPEGVTVLAAAKTRTLEMVREAYQAGLRHVGH
                     NYVQEAAAMIPAMREDIHWHMIGHLQRNKAKVAVELFSMIDSVDSQRLADELEKQALQ
                     SEKLLPVMIEVNIGEEEAKSGIEPDLAFQLARHIQGLAHLRLVGIMTMGPLAEDAEDS
                     RTYFRKTRMLYELIKREMPDVNYLSMGMSHDYLVAIEEGANMIRLGTAIFGERAKNR"
     gene            6986..7279
                     /locus_tag="PLL88_01750"
     CDS             6986..7279
                     /locus_tag="PLL88_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00316.1"
                     /translation="MDTIQVTVEFSGLSRILAKANEMQISLSPKQTYVDVIRTVAEKY
                     PSLVGDVIDPSKSVLFPSNVFSRDGKTVVLPEQMNDSPCDGEVLILLSLLAGG"
     gene            7300..9186
                     /locus_tag="PLL88_01755"
     CDS             7300..9186
                     /locus_tag="PLL88_01755"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOJ00317.1"
                     /translation="MYGYHGKVLHIDLKEKKHWTEQKPEDWYKKYIGGVSMATRLCWE
                     NIQPGCDAYSPDNPVCIANGIFAGTPVPVGGKFGLASKSPMTGFIGDSLSGSWFSIAL
                     KRAGWDGVVIHGISESWVNVFIDDDRVELRDATSLLGKGTYETEEAIREQLHDDQVRS
                     ATIGQAGENMVRFANVTNDGRQAGRTGHGSVWGSKKLKAVSVRGTHGIAVADPDTLMK
                     LSFNITKEAQGPHTSKYRILGTSTNVLNMNKLGLLPTRNYQEGVFEGAETVSGEYLHE
                     HHNVKTIACAQCPIACEQMSVVKSGPFEGAMTGIEYECLFANGPNLGINDLNYVIKLI
                     SVADHSGMDAMSMGITISWAMETYERKVFTDKDFKCKNHPEGFPLNFGNGQSAVFLAE
                     MIRDREGIGALLSEGTRIASRTIDKERGTETWKWAMQAKGLEPAGYDARGLKTFSLGS
                     AVGTRGPCHNRSAAYDVDIQGVVDRFKVDETRGEHAKDSEEYAAVYDTLPLCKFIRRC
                     FTGKADRAGAWPTIVQLINATTGWNLAYEDIDAIGARAVTMKKAFNIREGWKRTDDAL
                     PWRWMHDPMTKGPSAGYVTTEEELEYMKDLYYKAKGWTKDGLIPKENLIQLGMEDVAE
                     QIGV"
     gene            9198..9701
                     /locus_tag="PLL88_01760"
     CDS             9198..9701
                     /locus_tag="PLL88_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ00318.1"
                     /translation="MPTTTSKFQYVDCDPDKCIGCQLCEYVCSFTKTSEYNSYRSRIR
                     TVRVDEVLITAIACRTCENAPCVRACPKDALEQDPKTGIVRVDPTKCDACAWCVEACD
                     FGAISMNPATKLAEMCDQCETVEEGPQCVQWCPKEALFLITPDQRAQKSRRELIQEEI
                     IGSKAAG"
     gene            9755..10363
                     /locus_tag="PLL88_01765"
     CDS             9755..10363
                     /locus_tag="PLL88_01765"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ00319.1"
                     /translation="MAEQKPDDELRIGVYICHCGSNIAGVIDPAVVADWAAQLPGVVR
                     ATDTLYACADSGQSLIKEDIKKYDLNRVVVSACSVRMHETTFRAAVQEAGLNPFLMEM
                     ANIREQCTWAHGHDKEGALKKAKDLTAAAVAKACFLNPLTMIKVPVTKRAMVIGGGVA
                     GISAALDLADQGIETILVEKEPTIGGVMAQLNKTFPTMDCSI"
     gene            10379..12271
                     /locus_tag="PLL88_01770"
     CDS             10379..12271
                     /locus_tag="PLL88_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOJ00320.1"
                     /translation="MVDAARHPKVDVRTYTTVERVEGYVGNFKVQLREKARHVIADRC
                     NGCGECAKACPIEVPNYFEMNLAPRKAAYVPMSQSVPLRYTIDEDACIHCYKCVDACG
                     ALEAIDFSQEDKIAWEDIGSIVVATGFNHFDPSVIPEYGYSRYPNVITAMEMERLNNS
                     AGPTTGNLIRPSDQKNPQRLAIINCVGSRDKRFNPWCSNFCCMYAIKNAVLLKQMNPQ
                     LEISIYYMDIRTPSKGYEEFYDRARSMGIRFIQGRPSLITEDPKTRNLFVHAEDVVLG
                     KISELEYDMVMLNQAAVPQLDVDTVSSVLNITQSPGGWFMEYHPKLRPVDSPTDGIFL
                     AGACQGLKDIPSSVSSGLATASRAGRILHSDEWEIEPIIAQVWEDRCVMAQGKVCGVC
                     ARACPYGAIVIEEGKAASVTVAKCHGCGGCVAECPHNAITQMHFTDAQVLAQMRALLA
                     DKPEEKVFAFRCHWCSYGGADLAGTSHFDYSANERGIRVMCSARMDPDFLYEAYRLGA
                     GAVLYSGCHPQDCHYITGQKVGATRAERLAKIFEKMEMTPGRFRVEWISAAEGDKYAR
                     VINELQQVINDIPHDQLRQEIERLKPEMEKRARRMYETPGVDKAVEYSEEMARIITAQ
                     EVKSGA"
     gene            12261..12860
                     /locus_tag="PLL88_01775"
     CDS             12261..12860
                     /locus_tag="PLL88_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ00321.1"
                     /translation="MEHEKVAELRDLLIALPGCEKVDMCFSCGTCVSRCPIQQKVEPA
                     YNPRRLLKMVALNMQQDSFSSITPYLCSACDLCYAACPQEIHISAVLNGVKKLAVEAG
                     FATPLKTARVDALTCIGCGFCEKACPYEAISLKEVNVPMRGKGLLIAEVDAAKCMACG
                     TCSMICRSNSIELQAWENSEGVVDSIWSWFETAEVGGER"
     gene            12857..13291
                     /locus_tag="PLL88_01780"
     CDS             12857..13291
                     /locus_tag="PLL88_01780"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOJ00322.1"
                     /translation="MNQTRPAIVLFQCQYCLASSVDQYWVEHELPANVKLVKVPCSGR
                     IDPLYVLNAVQGGADGIMVCGCMPEKCHYKEGNLHARRVLAEFRNFLHLLGYSQERFV
                     FHWMHIDERGVIQSLLKDFEEKLEKMSPPVHLKTRAFSRENA"
     gene            13291..14268
                     /locus_tag="PLL88_01785"
     CDS             13291..14268
                     /locus_tag="PLL88_01785"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ00323.1"
                     /translation="MTNTRDQKIQAEARRLLQDGVVDVVIGFTRGTLPYRMQPCFIQE
                     ADETTCLEMNGLCSNNLAIYLNHRPRGERIAMVVRGCESRAIQALVLENRHARDALYL
                     IGVPCTGILDLQKVQPREQDAILSIQEEGDQVRVILSSGEKLLNRSDVLHSACVHCNH
                     PVPINADEIIDEPGLFEERSFDKSYLDAFRGLTLDERYERFREEAERCVRCYACREAC
                     PMCTCEECFVDHSRPQWTDSTICPAGQQAWHIIRAFHQTGRCVSCGACERACPMNIKM
                     TYLTDLLNRDVFEEYGFEPGLDPALMPPFLTLGQDKEKFTAQQEGQHAS"
     gene            14258..15274
                     /locus_tag="PLL88_01790"
     CDS             14258..15274
                     /locus_tag="PLL88_01790"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ00324.1"
                     /translation="MPAKWISKSDLKKLLAQWQKKHLVYAPMHNGHELAFQVVKDVED
                     MVLDGFLNTRYPAKSLFLPQSEALMKFDGILHSVETDEKEKIIFGLRACDAHALRLLD
                     HALVDEQNPDEYWEKKRAAAVVITLGCNSPDETCFCTAVGGSPFNTAGSDAMLVEMGE
                     EYLVYSISKKAEALFQDLPQADGSKTQNARKFMKGVETRMPGLFPKQELHDALMASFE
                     TDFWKAVSQSCLGCGVCTFLCPSCFCFDIVDETVRRERVRNWDSCMFRKYSLEASGHN
                     PRPTKIERTRQRVMHKFAYWVDLIDEMGCTGCGRCVRYCPVNIDIRAIATEALHREFE
                     GMKR"
     gene            15271..16116
                     /locus_tag="PLL88_01795"
     CDS             15271..16116
                     /locus_tag="PLL88_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOJ00325.1"
                     /translation="MKNCYEPIPMHIERVVIETSDASLKSFELTFNRKGDCEEFFSQY
                     RPGQFCQLSIFGKGEAPIGIASAAWEGDFVRFTVQKMGKFTSVIHEMHAGDAIGMRGP
                     LGNGFPLEEWKGMKLLIVGGGCAFSTLYAMTKHILHPAHRSDYADITALYGTRNSGLC
                     LYKPVIEEWHNRNDIVVHQAVDVAEEDWKYHVGYVPAVLEELAPKPDNTVAVLCGPPV
                     MTKFTIPVLTKLGFPPEIIYTSLEKRMKCGVGHCGRCNIGHKYVCKDGPVFTLQELQG
                     LPNEI"
     gene            16126..17088
                     /locus_tag="PLL88_01800"
     CDS             16126..17088
                     /locus_tag="PLL88_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ00326.1"
                     /translation="MSTRGLTQKTRTESELVLERSMITRHYVLRWDLERCVGCQLGPL
                     VCPKEALIHVDGVIENGRMVQKQSVDVDPQKCILCGECVEVCPVNAIEMLINGQPENP
                     VIEYEAFPKFEQSTTFDASVFDWKKKDFVIDNCPTAVIRENIQEHTLEVDDAHCIRCR
                     QCEIASDGAFRVLQPWQGSVVLQRELCVADCLACADICPTRALRVNEAGELVLADYFC
                     IKCGACVQICPVEAQYEEYEVKVESQGVTKVVKHQRVTNSKALPIWVERWRVHHQAVK
                     SGAWVQALLKLADEKAGMVEIDRKRALKRRDLILALKGGNQEKG"
     gene            complement(17120..18151)
                     /locus_tag="PLL88_01805"
     CDS             complement(17120..18151)
                     /locus_tag="PLL88_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00327.1"
                     /translation="MKKLLGVLFIIFLVLPLIFSTLMIYSISSWAFDRNLYRELFTSE
                     EVKAKLFSDEAIAKMLTEKLGAIEGIDTVAAVALVRSLVPEQSYTDQITGMVDQVFDF
                     MEGKSETLTLQIDLSEVKAAFNGPNQQALLEELAKVLPSCQNTQAAQGLLAALCKPAD
                     ISEKVFVEQFLKNNLPVLLAFMQNSISIFGPVTKADLTQNIPSFLHPYLNLASFRTTL
                     YIFAGITLLLWILTALLVGSNGRDRLLWLGWMLILPALAVLIIGLTVNTTLMRNLLEN
                     GMRQFYVLTTAMLPFSSQEILKTLQALFTEHIGDSFTLTGGFFSAIGLGFIAWGAATH
                     LPQTQDTEG"
     gene            complement(18270..19073)
                     /locus_tag="PLL88_01810"
     CDS             complement(18270..19073)
                     /locus_tag="PLL88_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOJ00328.1"
                     /translation="MTWFNEVFHKEKVMIASMYLPPLPGSPDYQKVTSLQQIIDYTHA
                     ELKALQEGGMDGVNIGNQQDWPYRVGVGPETPALISKILGEASQGMQIPIGISIFWDD
                     LAALAIAKAVGAKFVRGVFRGAYAGEMGLMSLNAADALRYRSQIDAEHVKLMFMLRPI
                     LCKSITDRSLQADVKDAMWSSKPDAFALCGPIPGESPTIDELKIVKEASKTCPVIMNN
                     GATPENIAEMLKHCDGAVVATHLRKDKKASNPFDRDKVRALMDIVEKSR"
     gene            complement(19131..20096)
                     /locus_tag="PLL88_01815"
     CDS             complement(19131..20096)
                     /locus_tag="PLL88_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00329.1"
                     /translation="MGKVRTVLGDIDSKDMGIVDAHEHLIRTGGLEILKNGEDWRLND
                     VAKAIEEVKLFAKAGGKTVVDMNPISNGRDIRMLVEIAKAVPEVHIMTCTGFHEGRIY
                     DNVIHWTATYEVNRIADLMIADIEEGIDIYDYNGPYVERSSAKASCIKIGTAYGLITP
                     VEERIIKAAAIAQEETGVCINTHTSGGTMAYEQAERLIGYGVKPEKIVIGHVQRNPDV
                     WYHKKIAAMGANFMYDGSYRIKYLPDSSRAMLIKELVKAGYQKHICLGTDAGRRTYQK
                     SWGSGTGIDYDETVFLPRLREEGVPEDAIEDIYINNPARYFSIDK"
     gene            20333..20641
                     /locus_tag="PLL88_01820"
     CDS             20333..20641
                     /locus_tag="PLL88_01820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ00330.1"
                     /translation="MMKTIKFLAICGNGLGSSLIVKMILEGVLADLNVRGIVESTSVA
                     QAAGMMPFADVVITSTAFYKGIESAIPEGKPVITCMNILDKEELSQRVAEVVKEKFSS
                     "
     gene            20729..22051
                     /locus_tag="PLL88_01825"
     CDS             20729..22051
                     /locus_tag="PLL88_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HOJ00331.1"
                     /translation="MQVLLDIWNVIFNQVVTKPQLLLAIIVAIGYTLLKRPFINVLSG
                     AIKTMVGVMILQVGSGQLTNTFRPILLALGERFGITGTIIDPYTGFPAALEALGDNQS
                     WVGYVILIALGVNILLVAVTKMRGLFLTGHIMFQQSCVATALIAYTLGLPMLPTILIT
                     GALVGLYWAIGSNALIGPTNKVTNNAGFTIGHQQMLMDWIASKFSHKFGDPEKDDCER
                     VELPGWFAILQDNIVAMAIIMFLFILVIMLALGKETVDGMAAGTNWILHILLTGVSFA
                     VYITIILTGVRLFVAELATSFKGISEKLLKNAVIGVDCPAIMPFSPNAWMIGFLFTTL
                     GELIAIGVLILMKSPVLIIAGFVPLFFDGGPIGVYANKHGGFKAVAFFCTLTGLVQVF
                     GSALIVPMSGMVGGYMGNFDWATVWPAIIVVLRFIGNLFGLPVPPYGA"
     gene            22191..22652
                     /locus_tag="PLL88_01830"
     CDS             22191..22652
                     /locus_tag="PLL88_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ00332.1"
                     /translation="MAATLLELLQENMIELNVPVGNWEEALQQGAQLLIDSGGVEPRY
                     LDAMIAMVKDLGPYVVIAPGLALGHAGPDQGVKHTCFSLVTLGTPVNFGVPENDPVDI
                     VFSFAAPNKEEHMQALRDLALFCSERENLDAIRAATDKKQVREVLIKFFHS"
     gene            22684..23652
                     /locus_tag="PLL88_01835"
     CDS             22684..23652
                     /locus_tag="PLL88_01835"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOJ00333.1"
                     /translation="MRLNSKEMNMIRAAELYYEQRLGQSEIAKILNCSRSTVSRLLAE
                     AVESGIVRIYIKRPVEKFPSLAEKVKHHFKLKDAIVVSGGTSNDQAFYNVGFAAAEFL
                     SQILHDHVVIGISFGVTLSYMVQELFEYTFEYDGIEVIQLMGGLGFGDPAIDGPELAQ
                     KMAQALGGTYRYLQAPAVVDSPEIAQHLLNEKTIRETIQKAFQAEITISSVGSLMDNL
                     SSLERSGYIRKEERATFQAKGAIGHSLSRMIDENGIPIHDPFNARIVGAPLDVIKNAK
                     WSIGIGSNPLKTQAFLAAVKDRQFNVLVIDDGTAREMLRLQAGTSE"
     gene            23867..24532
                     /locus_tag="PLL88_01840"
     CDS             23867..24532
                     /locus_tag="PLL88_01840"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOJ00334.1"
                     /translation="MSEIRKETRELGEFHAITVKGIGNIELKQGSVQQVTVEADEHLL
                     SRITTDVINGCLIIDIGRDWLERLSAGLDFLAARSINFYITMQQLDKLEVAGSCKIEV
                     TGFKGKELRLELNGASNMELADIQYEAVNTSLAGAGKLDLRGKTKEQAVTLAGAGSYV
                     ADDLESDRTRIALTGAGSARLWVTKELDASITGVGSIEYYGEPEVKQSLAMMGSIKNL
                     GKK"
     gene            24901..25434
                     /locus_tag="PLL88_01845"
     CDS             24901..25434
                     /locus_tag="PLL88_01845"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HOJ00335.1"
                     /translation="MITILQTKNETLVPIEQFADGAWMNVINPDERDIERLLSLLLED
                     VIIENQQALEMTGIAGNLLGQMMDAYASIIQNNQNIIFKIMTAWAIILSLPAIISGFF
                     GMNVGLPFESHPNAFMITTGILLAIMLVVMFSFGRSIGYENGSVRRGKKMSRSILTNS
                     IQSKRILGRREMERCSI"
     gene            25422..26681
                     /locus_tag="PLL88_01850"
     CDS             25422..26681
                     /locus_tag="PLL88_01850"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ00336.1"
                     /translation="MFYLSEILDKKVWDRWGKEIGKCRDILTGFDEKVFHRIVAVEIK
                     EKDGNTRLISSESVSSLFPNVTVSKPMIEVNPYTPKGNELYLRKQVLDHQIVDVEGKR
                     VVRVNDIQIAFTQNAYFVTGVDVGNAGLLRRLGLKKMNELFKKLAKSSSPEGVISWKD
                     VAYVEAKDPLRLKTSQEKISKLPPADIASILNDLDRPTGQSLLQRMGDEILADTLEES
                     PAKFQIDALSRMASERAADILEEMEPDEAADLIASLPQEQGSVLLDLMEKEDADDIRT
                     LLDYPPDSAGGIMTTEFAWIPRGLTVEKAMAFLRSSEDAQEVEDMYYIYVLDEKGKLA
                     GTVYLRDLVMAAPTMEVESMMEKDSIVVSPLATQEEAAYLVAKYNLLSIPVVDGETGI
                     MQGIVTLDDALDAVLPTAYKKRLPRFF"
     gene            26691..27944
                     /locus_tag="PLL88_01855"
     CDS             26691..27944
                     /locus_tag="PLL88_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOJ00337.1"
                     /translation="MKKATSNSTFFRKLWLLLTILGPGFITASADNDAPGIATYSMAG
                     SRYGYNFLWVLVAVTFGEVIVQEMAARMGTVTGKGMADLIREKFGVRTALFAMTCLLV
                     ANIGTTIAQFAGVAAGAELLGISRYIAIPVVGIGLSLMIMRGSYKVVEKILIILCMAA
                     LSYVVAAIALKPDWNEIARSAFIPKIVSLDSAYLLALLATIGTTITPWGIFYMQAAVV
                     DKGVKVEEYPMTKVDVVFGAVWGNIISAFIIITTAATLFKNGIVVEEAEQAALALAPL
                     AGEWAKVLFSIGFIGASILAATVLPLSTVYPIAEAFGWERGLDNSRKDAPIFYGLLAG
                     VIIVSAIVVLFPNLPLFQLMWTSQALNAVLLPVILILMLKLANNEELLGKYVNKKWQN
                     YFSYSLMVIICVATLVLFIDPWIAG"
     gene            complement(27957..29771)
                     /locus_tag="PLL88_01860"
     CDS             complement(27957..29771)
                     /locus_tag="PLL88_01860"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HOJ00338.1"
                     /translation="MKESSFSIEFQPIGIRLAFSEPVTILDAARQAGIQLRSDCGGKG
                     SCGKCVIRIMEGNSNPLTKSEETFRKGGVLLSDERLACEAILDGNAKVFIPTTSLSTA
                     QVLQTEGEFQSEHVQSPIHVEALQLTPPSLIAPRSDLENVLAASKTLHFENDEKALEI
                     ARTLPHQLRKNGWKVYAMCNEDTIIHVKPEKPRQVLGLAIDIGSTKIACYLMDLVSGG
                     TLAVKGIPNPQIAYGEDIIARLSYAMQGTEQEAQLHTALISAINQAIENITAISSSRI
                     DEIADMAVVGNTAMHHFFLHLPSKYLATSPFTPALTKEMYLSSEMLGVSGMPGSRVFV
                     PAPIAGFVGSDHLAFLLAAGFGEDETIRLGIDIGTNTEIALQKGKRIVSCSTASGPAF
                     EGAHIRYGMRASPGAIEHVSIKRDGQAKCAVIGEGEAVGICGSGILDAISEMKRVGIL
                     NQRGRLDTGHSHVAINEDGVRYYPLLPASESQREIPLSQKDIDQILLAKGAIRSGIAI
                     LMDKLGVQAEEIEEVLIAGAFGSYLNPYHAIQIGMLPVLKLSKIKAVGNAAGAGARLM
                     LLSEKMRQKAKALSEKIEHLELNTYPDFELFFANGIRL"
     gene            complement(29811..30572)
                     /locus_tag="PLL88_01865"
     CDS             complement(29811..30572)
                     /locus_tag="PLL88_01865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOJ00339.1"
                     /translation="MENKSYLIDMDGVLVYGKKMIPGADKFIQTLRDKNCKFLILTNN
                     SIYTPIDLAHRLQTSGLDVNEREIYTSAMATANFMKTQKAGGNAFVIGESGLNLAVHE
                     AGFILTPENPDFVVLGETFDYNFRQVTKAIRLILLGAHFLATNPDPTGPSDEGIVPAC
                     GAMATLIEKASGKSPFFCGKPNPFMMRSALNHLGVHSENTVMIGDRMDTDIVAGLQSG
                     METILVLSGVTTLKDIENFPYRPNHIYKSISEIEI"
     gene            complement(30686..31636)
                     /locus_tag="PLL88_01870"
     CDS             complement(30686..31636)
                     /locus_tag="PLL88_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HOJ00340.1"
                     /translation="MIVTTKKLFEAAYGKYAIGAYNINNLEQTMGLFRGNLMSKAPFI
                     IQISKGSRDYTDKEMLQSIIVAADKVFPEAVFAVHLDHGDEETCYDCINSGFYSSVMI
                     DASHLNFEENIATTKRVVETAHAKGLVVEAELGQLGGVEEHVQVSEEEAKLTNPAQAR
                     EFVDRTGCDSLAVAIGTSHGAFKFSGSQSLHFDVLAEIQKQLPGFPLVMHGSSSVPQD
                     EVERINKTGGKLQGAKGVDADQFKRAAELGVTKINIDTDGRLVWTRVHREYFIEHPEV
                     FDFRKPGKVFMDEYAKFIASKNEKLGSAGKLDEVRKLVSK"
     gene            complement(31732..32442)
                     /locus_tag="PLL88_01875"
     CDS             complement(31732..32442)
                     /locus_tag="PLL88_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOJ00341.1"
                     /translation="MNEQKPDHDISEHKRVNDILLGPLERPAIQWLVEHMPAWVTPDH
                     LTVIGLIAALVIGVSYWATRYSPNYLWLASFGFILNWVGDSLDGNLARYRKIERPKYG
                     FYVDHIVDTISEITIFLGLGLSQYVDFNLAVMGLIGYLCMTIQVYLTTNLRGVFKISY
                     GKLGPTEIRAIAILANTFIFFIGNPAIKLPFGVGEVGLYNLIVGTVVVLLYVIFMITV
                     FQEARLLDKEDRMKWKSK"
     gene            complement(32479..33384)
                     /locus_tag="PLL88_01880"
     CDS             complement(32479..33384)
                     /locus_tag="PLL88_01880"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00342.1"
                     /translation="MSFQDLINGEIGVNFAKGLAKRLPEKIGFPFAHFLAKVISQRKN
                     ASMVRCVRANQWVISGKKLTSQELDQRVLQVFDHTAYCLFDLYHNIDSPETLLKKITI
                     SPKMEEILASREKYQEGTIFLTPHLSNFDLAGRVITYKGHHILVLSYPNPNKGYQMQN
                     KLRKEYAIEIMPMSVESLRLGKQRLQEGHAIITGLDRPLSESKYHPRFFGYPSMLPVT
                     YVKLALQTHSPIVVVACVGQEDGRYTAESSDLIYLERYSDPVEEMERNAEKVLKQAET
                     FILAHPEQWSMFYPVWPWALDEMPQ"
     gene            complement(33470..34204)
                     /locus_tag="PLL88_01885"
     CDS             complement(33470..34204)
                     /locus_tag="PLL88_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HOJ00343.1"
                     /translation="MPCLEVIADTVEDAIAAESGGATRIAILSHYPSSGITPSYGMVS
                     RIREKVKIPLVVLICPHTRGYIPSHEDLEICLRDIDAFKKLGINDFLLGFIDQSSNLN
                     VAVIETIKAAHSGIHLHSRVIWELTRDIRKTVETTISLGFHSMRTSGNCLTNSLTNGD
                     FTKNLQNISLIKETAKDRIEILLTGGITARNVETFLRQTTIYNLQIGRGVRTPSNSHS
                     PVDPLKVAAIRSKQISIFEEHLLQSR"
     gene            34389..35063
                     /locus_tag="PLL88_01890"
     CDS             34389..35063
                     /locus_tag="PLL88_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ00344.1"
                     /translation="MDDSLNFHACIFDFDGILVDSEVVLVKLWKRIAGEYHYDFSEQS
                     FQKCLGRTTEDIKQIQMDEFGSQYPFDEIYQKVHDSFHQMITGHGLPLVPGVLVLRQF
                     LEDAQIKMAVASSTYRAEVLFRMEKAGLTDLFSTVLGGDDVLQPKPAPDIFLKAAAAL
                     QMEPADCLVLEDSQSGVLAARAAGMQVFVVQNLSPVTEVMREKAHAIFPSHAALLQFL
                     KGAGAA"
     gene            complement(35060..36010)
                     /locus_tag="PLL88_01895"
     CDS             complement(35060..36010)
                     /locus_tag="PLL88_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ00345.1"
                     /translation="MINKSEAAPRTPIAILGSGSIAQKAYFPLLTAWEGIEIAAIYSR
                     NAQHAMNACARWNLPYWSTSIDDLFKLNIKAAFVLTSKESHYSLIQTLLEDGVDVFVE
                     KPLTTTSQQALELAELAQKKKRILMVGFNRRYALLYRQAKQKLDGKEIQLIVIQKHRT
                     TPHYPNLTEFYLEDYIHQIDLLRYFCGEVEPLQTSFQMRDQTLHSCISTVLLPNGGLG
                     TLLSAHTAGIWQESVSIHADNLSIHVDAFRNLWVRYAEHEELFGSDRAGNWTPDLRER
                     GFIPEIEHFFHCIETRAEPESNGLEAAKTQQLLEKLLPGS"
     gene            complement(36000..37136)
                     /locus_tag="PLL88_01900"
     CDS             complement(36000..37136)
                     /locus_tag="PLL88_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ00346.1"
                     /translation="MKTYKIGLIGFGFIGKVHAQAYRSLPYCLADPPALARVMAVLRN
                     DPSKDKELLETLAVPICTNDEQLFWSQELDAIDICSPNASHYEYCLKAIKKKIPIYCE
                     KPLTKTIEDARELAKLVREQEIPTHTAFTYRFLPAVGLAKDLIAAGWLGEIHHFQSRL
                     FHSSYLNPQRPISWRLQEDIAGGGALTDLGIHFLDLIRNLLGDVEWLQCQAETFIKSR
                     PRQDDPNSYEEVCVDDWALCTMQLQNGGIGSLEVSRVAGGAGNIACLEIFGSRGSIKI
                     DFNQSETIQYYDVKKDQWISSAVLPEGITPRHFDRSRWSENKRSLGRFLSAHIAVIST
                     FLDCLEKQIPSQADFQTALKAQELLHACYQSAREDGKRVFIHDQ"
     gene            complement(37224..38375)
                     /locus_tag="PLL88_01905"
     CDS             complement(37224..38375)
                     /locus_tag="PLL88_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ00347.1"
                     /translation="MKTEEKLQLLTLHNVHEMDEEHCQFRKSNAIQDVCVSHATLPNG
                     KKIRLFKSLITSNCKNNCYYCPIRKDNDQPRAAFQAEEYARLINNLYHAGLIDGAFIS
                     SAIQENSVRTQDLLIKVTEILRLKMDFRGYLHVKFMPGLEFEQAKHIMYLADRVSLNL
                     EAPNAERIRYLSPDKTPFDQLIDPLKWTNTVRQNEATSANWKGHWPSVCTQFVVGGSE
                     EMDKELLQTTCYLYTELHLQRTYFSPLTPYKGTPLESHAPIPLKRKFRLYQASFLIRD
                     YGYSLDDFYFDHKSNLPLEKDPKLLWAEMNLRENPIEINQAEPARLMRIPGIGEKGAG
                     KIIQMRKIHPFRTESDLAKIGIQIQKGLPFITMNGKRPAVQPALPGLLQ"
     gene            38596..38808
                     /locus_tag="PLL88_01910"
     CDS             38596..38808
                     /locus_tag="PLL88_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOJ00348.1"
                     /translation="MHLGYLEIFVLIVLVLLLFGPGRIAKVAGELGQGIKAFKEGLSG
                     SKSEEKSAKSEKLDNQPEDEHQSKKD"
     gene            38824..39177
                     /locus_tag="PLL88_01915"
     CDS             38824..39177
                     /locus_tag="PLL88_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOJ00349.1"
                     /translation="MQLFGGVGLSEIIFILVLMIVVLGPKKMAEGARDLGKTIRKVTH
                     SQFWKNVMQTSREIKDLPRKIMDETELEDNLREINRAINQDSANSREGLSSPPEFKPP
                     HEQAEKSDRPDRTER"
     gene            39180..39908
                     /gene="tatC"
                     /locus_tag="PLL88_01920"
     CDS             39180..39908
                     /gene="tatC"
                     /locus_tag="PLL88_01920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOJ00350.1"
                     /translation="MPTEDETLMPFWAHFEELRKRLLIGLVALVIATFVCFSFAEEAI
                     RILAQPVGGVTALQSIEVTENIGVFMRVSLMCGVILASPVILYELLAFVMPGLKPGEK
                     RWVFLGLSIGTVFFVAGVLFAFYIMLPRTLEFLVSFMNVQTVPRFSGYIKFVTNLLFL
                     IGVSFEMPLIVFILAKIQVVNAGMLLKQWRYAIILIAVLSAVITPTVDPVNMGLLMVP
                     LIGLYFVSILFAFLAKGKEAKKSD"
     gene            39956..40930
                     /locus_tag="PLL88_01925"
     CDS             39956..40930
                     /locus_tag="PLL88_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ00351.1"
                     /translation="MKKRRLGNSAFFISAIGLGCWQFSKNRGLAGSFWGDLADDTITS
                     IIEASLRGGVDWFDTAELYGHGMSERMLSSALQRLNVEKEKIVVATKWNPVLRSARSI
                     VRTFPVREKYLAPYPITLLQVHNPFSVSPIEDQMRAMAFLVESGKVGLIGVSNFSLTL
                     MRKAHETLLADGFVLTSNQMRYNLLDRSVEFNGVLDYAREHDITLIAYSPLAQGLLTG
                     RFHEEGGLIQKRPGFRRYLPAFQQRSLLRTLPLIQVLRDIAERHQKTPAQIALRWIIQ
                     FHGEAIVAIPGASSVLQAQQNAAVMDFELTEEELAQIDETSLAVARRS"
     gene            complement(40925..>41872)
                     /locus_tag="PLL88_01930"
     CDS             complement(40925..>41872)
                     /locus_tag="PLL88_01930"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012897.2"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOJ00352.1"
                     /translation="PIGVDVQGKPVFLDEILPSPAEIREYLKAIDASVYSEVYAQGLV
                     GKKKWFELEQKSGYLFDWEPDSSYLLEPPFITDFFNTQNDVCDARVLAYLGDSITTDH
                     ISPAGNIATTSDAGEYLLTFGCDPADLNTYGSRRGNHEVMSRGTFSNPRLQNKLLAEK
                     AGGFTSIYQAARLYKDRHIPVIVIAGKQYGTGSSRDWAAKGPFLLGVRTVLAESFERI
                     HRSNLVMMGILPLQFTNGENAHTLGIQGTEIFSMRGIEKLNHTRQLITVEMKRADEQI
                     SVFQVIARLDTPMEVQYYRKGGILPTIFSDICAMEERSS"
BASE COUNT        10268 a         9595 c        10994 g        11015 t
ORIGIN      
        1 tgcgccgata tatgcccaac agatgcgatc ggtctcaagg acaaccgggt gttttttcgg
       61 aatcctgcga actgcacgta ttgcacgttg tgtgaacaga tatgcccgca gcaggcgata
      121 cggtgcgaat tcaccattac cgtacaggaa aggaacaggt aacgatgagc gaatacatca
      181 acaattcaag ccaacgtgaa gaacaattaa aggagatcat taaacaatta catgccgggc
      241 gagcgtttgc tgacctgaaa gatacctttc aggatctttt aaaggacgca tcggctcaag
      301 agatcgtgca gatcgagcaa gcgctcattg ctgagggctt gcctgtggag cagatccaac
      361 tgctgtgtga tgtgcatgtg gcattgttca aagaatcgct ggatacccag cttccgcctg
      421 aaatgcagcc cggtcatccc atccatacct tccgctctga aaatgagctg gtggagttgg
      481 tgctgcatag catggatgaa aaagttgcca aattgaccct atcggatgat caggagttgc
      541 gcaatcagct ctcgaacgat gttgaaaaac tcaagcaaat ggaccgtcat tatttgcgca
      601 aggagaacct gctttttccc attctggaac gatacggttt caccggaccc tcttccgtga
      661 tgtggggaat ccatgatgag attcgcgcag gttggaatga gcttggtaaa ttgatcaccg
      721 acgcccctgt tgaccaattc agtgagaagt accagcaatt gcagcacaag atccgagaaa
      781 tgatttataa ggaagaccgt atcttattcc cgagcgcgtt ggaacgtctt acagaccagg
      841 aatggggaac cgtatatgcg ggagaagcgg agctcgggtt tgcttatgtc acgcgcggca
      901 atgattggaa accgcagatt caagcaaatc cggtaaaatc agaagtggat tatcgggtga
      961 acaaggatga aaaggagatg gatatggttg aattgacatt gaacgttgga aaattaaccg
     1021 caaaacaggt gaatctgctg ctgcaaaacc tgcccattga tgtgaccttt gtggatgaga
     1081 atgaccaggt ggctttctat tcccaatcgc gtgagcgcat tttcgcccgc actcccgcag
     1141 tcattgggcg taaagtgcaa atgtgccatc cgccagccag cgtggataaa gtgcagaaga
     1201 ttctggatga tttccgctca ggcaagcgca gcgaagctgc cttttggatt cagatgaaag
     1261 gaaagttcat tcatatccgc tatttcgcgg ttcgcgatga agagaatacc tatcggggaa
     1321 ccatcgaagt gacccaggat atcactgaga tccaccaatt ggaaggggaa aaacgtcttt
     1381 tggatgacta actgagccaa tgcaatttat tggaaaaaat gacccatttt tcacgggtca
     1441 tttttctttt gacagtcagg gcaaatgccg aataacacca agtgattcgt ggtgatctgg
     1501 tatccgcttt tggcgtgcac acggttgagg aaatcatgca tctcaccccg ctgaaaagtg
     1561 agaactttcc cgcatttttc gcaaaccaaa tgatggtggg gttcttttgc cacaagttca
     1621 tataccacag ccggaccgcc catatcggat acagaaacct gaccttgatg taccatgcgt
     1681 tccagagagc ggtacactgt ggatggatcg acagcgctca gggtaccctt aactttttcg
     1741 tacacctctt tggcagttaa atgatcttcc tggtcttcca gaaccttaag aatcgcattg
     1801 gttattgagg atgctcgcat ttttgtctct tcaatagtat aatatgcaaa tctttgcaat
     1861 tacaaaacta tgcataggag gtcatattat gtttgtaaag cagcaactca tgcacattcc
     1921 cgatggtttt ctctctttta ttttatcgct tattttgtgg attctatctg gcattgtgat
     1981 ctttttggcg atcaggcatt atgcgaaaga gaaggaaaac cagaagttac ccctcatggg
     2041 tatattggcg gcggtcattt ttgccgggca gatgctcaat tttaccgtag tgggagggac
     2101 ctcgggtcac cttttaggag ctgcgctggc aaccatcctg gttggtccct gggaagcagt
     2161 gctggtaatc acgagcgtgg tggcggtgca ggctatcgta ttccaagacg gtggcttgct
     2221 ggcactgggt gccaatatct ttaatatggc tgtgattggg gtattggttt cttatgcggt
     2281 ctattctctg ctgtccagga ttatcaagaa agggaaatgg tctgttggga tcgcaggttt
     2341 tctggctggt tggctttcga tcttcgtggc agcgctggca gccgccctgg agttggctgt
     2401 ttcgggaact tcgccggcta atatcgccgt tcctgccatg gctggcatcc atgcgttgat
     2461 cggcataggg gaaggattga tcacagcagc cgctttgcta tttgtcgctg ccacgaagaa
     2521 ggagctgctc gagggatctc aaaagcccaa agcggtagat gcgtccattc ttatcggggg
     2581 aatcctgatc gcattggttt tggctgttct ctcgcccctg gcttccgctt atccagatgg
     2641 gctggaaaga gttgccgaag acctcggatt tctttccaaa aggcagggac ctctgtatac
     2701 cgtcattcct gactacacct ttccgggcat ccacgatgag agactggcaa ccatcgtagc
     2761 cggagttctg ggagttgtgt tggtcgcttc cgtggcgtta tttacccatc gcggaaaaaa
     2821 gaacagcgcg caaaagtagt ttcatttggc tttgtaccat ccaggaagcg attttctgtt
     2881 ttcggtagac acccgggtga aatgggtggg ggttatcatg atcgcgttca ttgtgctaac
     2941 cattcccctt ttcacctggg ctgtttatat tttgctcttt gccattcttg tggcactgtc
     3001 catttcctcg cgcctgccgt tcaaacagat cctgaaaagg accttgttgg tcgaggtgcc
     3061 actgttgttc atactgatcc cgcttccctt tatcaatccc agtacagcca ccacaaggat
     3121 ttccttgttg ggattatcta ttgacttctc attgcctgaa ttgttacggg tgggcgttct
     3181 gttgttgcga tcgtggctga tcattttcag tatggtggtg cttaccgtga ccaccccggc
     3241 agatgagttg ttggcatccc tctccagcct gggtgtgcct gcggtgctgg tcacgatcat
     3301 tttgctgatg tggcgttatc tggttctttt cgtttcgaag gctaaaaaac tttccgccgc
     3361 tcgtgaattg cggagtgctc tcccgcagga tggaaattac aaaaactggc acaggcttcg
     3421 cctcagcgtc caaagcactg gatccatgct gggcggactt tttttgcagg cgtatgagca
     3481 aagcgatcgg gtttaccagg ctatgcagtt gcgcggtttt gatggcacat tgcgttcacg
     3541 aaagccagag ccattgcgca cctctcaatg ggtccagatt tttctcttgc ttgttttcgg
     3601 attgtccctc atactaggag cctatggtat ctatggaaag tgaacgaaca ccgattctgc
     3661 aagtgcgcga cctgcgtttt gcctatcctg acatgcctga aacgttaaac gccatttctt
     3721 tttctgtggc gcggggcgaa aaggttgcca tcgccgggcc gaatggagca ggaaaaacaa
     3781 ctttgttgct gaacctgacc ggtttgctga agggcgaagg ggaagtgtat gtcgaaggct
     3841 tgcgtttgga gaagggaaac ttttcagcca ttcggcgcat tctgggattg gtctttcaat
     3901 ctcccgatga ccagcttttc tcaacccggg tttttgacga tgtggcatac gggctggtat
     3961 accagggtat ggaaaaggct gagatcgagg agcgtaccag aaaagccctg gcagacgtgg
     4021 gaatggctgc ttatgaaagt gccgttcctc atcatctcag tttggggcag aagaagcggg
     4081 ttgccattgc gagcgtgctt tgcatgcagc cagagatcat cctttttgat gagccgacca
     4141 tggggttgga tgcgcgggca aagaaacaat ttcttgaaat tgcagcctcc ctcacccaaa
     4201 caatgctggt ggcgacccat gatctgcggt tggcggtagc gctctgcgaa cggttgttga
     4261 tccttgatga gggcaggttg gtttttgatg gagaaatgaa gaaagctctg cgggatcgag
     4321 aatttcttga acgccatggg ctagactggg atgtgtgaac gctgcgatcg gggttttcga
     4381 taaaaaagat cctcccttac aaaattaaag gaggatcatt gtgctgactt caaggatctt
     4441 ttatttcagc aaaatctcac tcaattcttc ttcatcaaaa tctaagatga tggtgccatc
     4501 gatgtcgaag gtgggagtga tctgcgagcc gtttccccac gatcttactt gcctggcagc
     4561 ggtgaagttg gtggtgatat cgacttcggt gtaaggaatg ttgttctctt tcaaccagat
     4621 gcgcgctctg cggcagtcgg gacaccactg ggtgcagtac attacgaccc caccgtgcgg
     4681 cagcgattca tcttgtggta agatctcgtc atcgaagaat ttctcagggg tgatcttctt
     4741 gatttcattc agcaacacct cattgctggg ttgatcatcg atatcatgaa tgtcgatata
     4801 gcggatgata ccttcttcat ccagcaagaa gatcgcccgt tcagtgtagc cttccgaacg
     4861 caacacgcca tatttttttg cgaccttgcc gtgcggccaa aaatcactta ataaaggata
     4921 ggtgatgcca cccaagctgt ctgcccacgc ttttaagcag ggaacatgat caacgctgat
     4981 gcccagaacc tggacattta atcccgcgaa tttcggcagt aaaacctcat acgacgggat
     5041 ctgccctgat catatggggg tccaggctag tggaaagaaa gccatgacaa cggtttttcc
     5101 gcgtagatcg tgcaaagtca ggtctttttc taaatggcta ggcagggtaa aatcaggagc
     5161 gtgttgacct acttttaatt tcatgtgtac ctccttgaat gggataaacc gtgaaaacag
     5221 ggtaaggttg acaaattcat tataacaaag gataaatcag atgtataagg tagtcttact
     5281 tcgacatggt gaaagcgaat ggaataaaac caatctgttc accggttgga cagatgtaga
     5341 tctttccgaa aaagggctgc aggaagcgaa gcaggctgcg gagctcttga aaaaggaagg
     5401 atacaccttt gatattgcct acacatctgt cctcaaacgt gccattcgca ccttatggat
     5461 cgtcctggat gggatggatc tgatgtggat accggtaata cgctcctggc gcttgaacga
     5521 gcgcatgtac ggtgcgctgc aagggttgaa caaagcgcaa accgccgaga agtatggaga
     5581 agagcaggtg catctctggc ggagaagtta taatgttccg ccgccggctc tggaaaagag
     5641 cgatgaacgc tatcccggtc atgaccgccg ctatgcagaa ctggcagaaa aagatatccc
     5701 cacaaccgaa tgccttaaag acacggtgga gcgctttttg cctttctggg aaaacaccat
     5761 tgctccaatg atcaaaacag gcaagcgcgt catcatcgcg gcgcatggca acagtttgcg
     5821 cgcattggtg aagtatctcg acaacatttc tgacgaggag atcccggaat tgaacattcc
     5881 caccggtgtg cccctgatct atgaactgga cgatgacctg aagcccatcg cgcattatta
     5941 tctgggcgat cagacagctg tggaaaacgc catccatgca gttgccaatc aagccaagaa
     6001 agaataaaga aagaccagct taagagcgct atcacagcgc tcttttgttt gatatcagga
     6061 agttcaacct atctctgagt ttttcgatta cactatacgg ggaatatggc tatgatcacg
     6121 gaaaatgtcc agagggttct gaagtgcgtc ccagagggag ttaccgttct cgcggctgcc
     6181 aaaacccgca cgcttgagat ggtgagggaa gcatatcaag ccgggctgag gcatgttggg
     6241 cataattatg tacaagaagc ggctgctatg atccctgcca tgcgcgaaga tattcactgg
     6301 cacatgatcg ggcacttgca gcgcaacaaa gccaaagtgg cggtggaatt gttttccatg
     6361 atcgacagcg tggattcaca gcggctggcg gatgaattgg aaaagcaggc gctgcaaagc
     6421 gagaaactgc tgccggtcat gatcgaagtc aatatcggag aagaagaagc caaatcagga
     6481 atagagcctg atcttgcttt ccagcttgcc cggcatatac agggtctcgc tcatcttcgg
     6541 ctggttggca tcatgaccat gggtcctctg gcagaggatg ccgaagattc acgcacctat
     6601 tttcggaaaa ctcgcatgct ctatgaattg atcaagagag aaatgccgga tgtgaactat
     6661 ttatccatgg gcatgagcca tgattacctg gtggctatcg aagagggggc gaacatgatc
     6721 cggctgggca cagccatctt tggcgaacgc gcgaaaaatc gctgacggta tcgtttcttg
     6781 catcatcttc ctgaacgatc aaaaatatga taaaaatagt cttaatatag gaaagttaac
     6841 agcataacac cctttttctc agccacattc ccaatattcc tatattataa tttttagagc
     6901 attgtcagat gttccagaca ctatttttct tcccactgct gtttgacact gcattttgat
     6961 gaaaaaaaag gttaagatta caggcatgga tacgattcaa gtaacggtcg aattttccgg
     7021 actgtcacgt atcttggcaa aagccaatga gatgcagatt tccctttctc cgaaacaaac
     7081 ttatgtcgat gttatccgca cggtcgcaga gaaatatcca tctctggtag gtgatgtgat
     7141 tgatccaagc aagagcgttc tattcccctc caatgttttt agccgggatg gaaaaacggt
     7201 tgtattgccg gagcaaatga atgatagccc gtgtgatgga gaggtgttga tcttgctttc
     7261 attactggcg ggtggttaga tcatacaagg agatgagata tgtatggcta tcatggtaaa
     7321 gttctgcaca tcgatctgaa agagaaaaaa cattggactg agcagaagcc agaggattgg
     7381 tataaaaaat atatcggggg cgtctccatg gcgacccgcc tgtgctggga aaacattcag
     7441 cccggctgcg atgcttattc gcctgataac cccgtttgta ttgccaatgg catctttgcc
     7501 ggaacgccgg ttccggtggg tggaaaattt ggtttggctt caaagtcgcc catgaccggc
     7561 ttcatcggag atagcttatc aggcagttgg ttttcgatag ccctcaagcg tgctggctgg
     7621 gatggcgtgg tcatccatgg tatcagcgag tcttgggtca acgtttttat cgatgatgac
     7681 agggtggagc tgcgtgacgc gacttctctg ctcggaaaag gcacctacga aaccgaagaa
     7741 gccattcgcg aacaactgca cgatgaccag gttcgttcgg ctaccatcgg gcaggctggt
     7801 gaaaacatgg tacgctttgc caatgtcacc aacgacgggc ggcaggctgg gcgcaccggt
     7861 catggttcgg tgtggggttc gaaaaaatta aaagcggttt ccgtgcgggg cacgcatggt
     7921 attgctgtgg ctgatccgga taccctgatg aaattgtcct tcaatatcac taaagaagct
     7981 cagggaccgc atacctccaa ataccgcatt ttaggaacct caaccaatgt gctcaatatg
     8041 aacaaactgg gtttgctccc cacccgcaat tatcaggaag gtgtttttga aggagcggag
     8101 acggtcagcg gtgaatatct gcatgagcac cacaacgtga aaaccattgc ctgtgcgcag
     8161 tgcccgattg cctgcgaaca gatgtcagtg gtaaaaagcg gacccttcga aggagccatg
     8221 acgggcattg agtatgaatg cttgtttgcc aacggaccca acctgggcat caatgacttg
     8281 aattatgtca tcaagctgat ctccgttgct gatcattctg gcatggatgc catgagcatg
     8341 ggcattacca tatcctgggc aatggaaacc tatgaacgaa aagtctttac ggataaggat
     8401 ttcaaatgca agaatcaccc tgaaggattt cctttaaatt tcggcaatgg acaatctgcc
     8461 gtattcctgg ctgaaatgat tcgcgaccgc gaagggattg gcgcgttgct atctgaaggc
     8521 acgcgcatcg caagtcggac catcgataaa gaacgcggta cagaaacctg gaaatgggca
     8581 atgcaagcga agggattgga acctgcaggg tacgatgcgc gcggattgaa aactttttct
     8641 ctgggttctg cagtaggcac tcgaggacca tgccataacc gtagcgccgc gtatgatgtg
     8701 gatattcaag gcgtggtgga tcgcttcaaa gtcgatgaga ctcgcggtga gcatgcgaaa
     8761 gattcggaag aatatgcggc ggtttatgat acgctgccgc tctgcaagtt tatccggcgc
     8821 tgttttacag ggaaagctga tcgcgctgga gcctggccaa ccatcgttca attgatcaac
     8881 gccacgaccg gttggaatct ggcatatgaa gatattgatg ccattggtgc cagagcggtt
     8941 accatgaaaa aggcgttcaa tattcgcgaa ggttggaagc ggactgacga tgctttgcca
     9001 tggcgctgga tgcacgatcc catgaccaag ggtccttcag ccggctatgt cacaaccgaa
     9061 gaggaattgg aatatatgaa agacctgtat tacaaagcca aaggttggac gaaagatggt
     9121 ctgataccga aagagaattt gattcagctc ggcatggaag atgttgccga gcagattggt
     9181 gtgtaacgga gaagaggatg ccaactacca cttcaaaatt tcaatatgtg gattgcgatc
     9241 ctgataaatg catcggctgt cagttatgcg aatatgtttg ctcgtttacc aaaactagtg
     9301 aatataattc ttaccgtagc cgcattcgca cagtgcgcgt ggatgaggtg ctgatcacag
     9361 ccattgcctg ccgaacctgt gaaaatgcgc cctgcgtgcg cgcctgcccg aaagatgcat
     9421 tggaacagga tccaaagaca gggatcgtcc gcgttgatcc taccaagtgc gatgcgtgcg
     9481 cctggtgcgt ggaagcctgt gactttggtg ctatatccat gaacccggca acgaaattag
     9541 ctgaaatgtg cgaccagtgt gaaaccgtag aagaaggccc tcagtgtgtg caatggtgcc
     9601 cgaaagaagc attgttcctc atcactccgg atcagcgagc gcaaaaatct cgcagagaat
     9661 taatacaaga agagatcatc ggcagcaaag cggctggttg atcggcaatt tcctccctgt
     9721 gttcgaaagg gagtgtgttc attaaaggag caagatggca gaacagaaac cagatgatga
     9781 gctacgaatt ggcgtgtata tttgccactg cggttcaaac attgccggtg tgattgatcc
     9841 ggctgtagtt gctgactggg ctgcgcaatt gccaggtgtg gtgcgcgcaa ccgataccct
     9901 gtacgcctgt gccgatagcg ggcaatcgct gatcaaggaa gatattaaaa aatacgatct
     9961 caaccgtgtg gttgtatcag cctgctcagt gcgcatgcac gaaaccacct tccgcgcggc
    10021 ggtgcaagaa gcggggttga accctttctt gatggaaatg gcgaacatcc gcgaacaatg
    10081 cacctgggcg catgggcatg acaaagaagg agctttgaag aaggcaaaag acctgacagc
    10141 cgcagcggtt gccaaagctt gcttcctcaa tccgttgacc atgatcaagg tgcccgttac
    10201 caaacgggct atggttatcg gtggaggggt ggctggtatc agcgcggcgc ttgatctggc
    10261 agatcagggc attgaaacga tcctcgtgga gaaagaaccg accatcggtg gggtcatggc
    10321 acagttgaac aagacctttc ccaccatgga ctgcagcata tgaattcttg caccgaaaat
    10381 ggttgacgcg gcgcgtcatc ctaaagttga tgtaagaacc tacaccactg tagagcgtgt
    10441 agaaggatat gtaggcaatt tcaaagtaca actgcgagaa aaagcacgcc atgtcatcgc
    10501 cgatcgttgc aatggctgcg gcgaatgtgc caaagcctgt ccgatcgaag tgcccaatta
    10561 ttttgagatg aatctggcgc cgcgcaaagc agcgtatgtt cccatgagcc agtccgtccc
    10621 cctgcgctac acaattgacg aagatgcatg tattcattgt tataagtgtg tggatgcctg
    10681 cggtgcattg gaagctattg atttcagtca ggaagataag atcgcctggg aggatatcgg
    10741 ctccattgtt gtggctaccg gtttcaatca ttttgacccc agcgtgatcc ctgaatatgg
    10801 atacagccgc taccccaatg tgatcactgc tatggagatg gaacggctca ataattctgc
    10861 tggtccaacc accggtaatc tcatccgccc cagcgaccag aaaaacccgc aacggctggc
    10921 gatcatcaac tgcgtcggct cgcgcgataa acgctttaat ccgtggtgct cgaatttctg
    10981 ttgcatgtac gccatcaaaa acgcagttct gttgaagcaa atgaacccgc aattggagat
    11041 atccatttat tacatggata tccgcacgcc ttctaagggc tatgaagaat tctatgaccg
    11101 cgcgcgcagc atgggcatcc gcttcatcca ggggcgtccc agtttgatca cggaagatcc
    11161 caagaccagg aacctgtttg tccatgcgga ggacgttgtg ctgggcaaga tcagtgagct
    11221 ggaatatgat atggtcatgc tcaaccaggc ggcggttccg caactggatg tggatacggt
    11281 cagctcggtg ttgaacatca cccaaagccc gggcggctgg ttcatggaat accatcccaa
    11341 actgcgcccg gttgatagcc ccaccgacgg tattttcctg gcaggcgctt gccagggtct
    11401 gaaagatatc ccatcttctg tctcttccgg gttggcaacc gcttcccgcg cagggcgtat
    11461 tctccattcg gatgagtggg agatcgagcc gattatcgcg caggtttggg aagaccgctg
    11521 tgtgatggcg cagggtaagg tatgcggtgt gtgcgcccgc gcctgcccgt atggtgcgat
    11581 cgttattgag gagggcaaag cagcttctgt gacggttgcc aaatgccatg gctgtggtgg
    11641 ttgtgtggcg gaatgtccgc acaatgccat cacacagatg cacttcaccg atgcgcaggt
    11701 gctggcgcaa atgcgtgccc tgctggctga caaacccgaa gagaaggttt ttgccttccg
    11761 ttgccactgg tgttcgtatg gtggcgcgga cctggctggc accagccatt tcgattatag
    11821 cgctaacgag cgcggcattc gtgtgatgtg ttcggcgcgc atggatccgg actttctcta
    11881 tgaggcttat cgattgggcg ctggagcagt gttgtattca ggctgccacc cgcaggattg
    11941 ccactatatt accgggcaga aggtgggtgc cacccgagca gagcgcctgg caaaaatctt
    12001 tgaaaaaatg gaaatgaccc ccggtcgttt tcgagtggaa tggatcagcg ccgccgaagg
    12061 tgataagtat gcgcgcgtga tcaatgaatt gcagcaggtc attaacgata tcccacatga
    12121 tcagttaagg caggagattg aacgcttaaa accggaaatg gaaaaacgtg cccgccgcat
    12181 gtatgaaaca ccgggagtag acaaggcggt agagtactct gaggaaatgg cgcgcatcat
    12241 cacagcacaa gaggtgaaga gtggagcatg aaaaagtagc cgagttgcgc gatctgctga
    12301 tagcacttcc gggttgtgaa aaagtagata tgtgcttttc ttgcggaacc tgtgtgagtc
    12361 gctgcccaat ccagcaaaaa gtcgaacccg cttataaccc gcgccggctg ttgaagatgg
    12421 tcgcattgaa catgcagcag gattcttttt catcaatcac cccctacctt tgttcagcct
    12481 gtgatctgtg ctatgcggct tgcccgcaag agatccacat ctcagcagtg ctcaatgggg
    12541 tgaagaagct ggctgtggaa gctggctttg cgacaccctt gaaaacagcc agggtggatg
    12601 cgctgacctg catcggatgt gggttttgtg aaaaagcttg cccgtatgaa gccattagcc
    12661 tgaaggaagt caacgtccct atgcgcggga aagggctgct gatagcagaa gtggacgcag
    12721 cgaaatgtat ggcttgcggc acctgctcca tgatctgtcg gtcgaactcc attgagctgc
    12781 aagcctggga gaatagcgaa ggggtggttg attctatatg gtcgtggttc gaaacggcgg
    12841 aagtgggtgg tgaaagatga atcaaacgag accagccatc gtcctgttcc agtgccagta
    12901 ctgtctggct tcaagcgtgg accaatattg ggtggaacat gaactgcccg ccaatgtgaa
    12961 attggtcaaa gttccctgct ccgggcgcat cgaccccctg tatgttttga acgccgtgca
    13021 gggcggcgcg gatggcatca tggtctgtgg atgtatgccg gaaaaatgcc attataagga
    13081 gggcaacctg cacgcgcgca gagtgctcgc tgaatttcgc aattttcttc atttgttagg
    13141 atactcgcag gaacgttttg tctttcattg gatgcatatt gatgagcgtg gcgtcataca
    13201 atctttgctg aaagattttg aagagaagtt ggaaaagatg agtcccccgg ttcatttgaa
    13261 gacacgcgct ttcagtcggg agaatgcctg atgaccaaca cacgggatca gaaaattcag
    13321 gctgaagccc gacgactgct ccaggatggg gtggtggatg tggtgatcgg tttcacccga
    13381 ggaactttgc catatcgcat gcaaccctgt tttatccaag aggctgatga aacgacttgc
    13441 ctggaaatga acggtctgtg cagcaacaat ctggcaattt acctgaatca tcgccccagg
    13501 ggcgagagaa ttgccatggt cgtcaggggt tgtgaaagcc gcgctatcca ggcgctcgtt
    13561 ttggaaaacc gccacgcgcg ggacgccctc tatcttatcg gagttccctg caccggtatt
    13621 ttggatctcc aaaaagtaca accgcgagaa caggatgcta ttctttctat acaggaagag
    13681 ggcgatcagg tgcgtgtaat actgagcagc ggtgaaaagc tcttgaatcg atctgacgtt
    13741 ctccatagtg cctgtgtgca ttgtaaccat cctgttccaa tcaatgccga tgagatcatc
    13801 gatgaaccag gattattcga agaaagatcc ttcgataaat cgtacctcga tgcgttccgc
    13861 ggcttgacgt tggatgagcg ctatgaaaga tttcgcgaag aagctgagcg ctgcgtgcgt
    13921 tgttatgcct gtcgggaagc ctgccccatg tgtacctgcg aggaatgttt tgtggatcat
    13981 tcccgcccgc agtggaccga cagcaccatt tgccctgccg gtcagcaagc ctggcatatc
    14041 attcgcgcct ttcaccaaac cggaaggtgt gtgagctgcg gcgcttgcga acgtgcctgc
    14101 cccatgaata tcaaaatgac atacctgacc gacctgttga atcgggatgt gtttgaggaa
    14161 tatggctttg aacccggttt ggaccctgcg ttgatgccac cattccttac cctgggtcag
    14221 gataaagaga aattcactgc acagcaggag ggtcagcatg ccagctaagt ggatctctaa
    14281 aagcgatttg aaaaaattgc ttgcccagtg gcaaaaaaaa cacctggtgt atgcacccat
    14341 gcacaacggg catgaactgg cttttcaagt agtaaaggat gttgaggata tggttttgga
    14401 tgggttcctc aacacacgct atccagccaa atcccttttc ctcccgcaat cggaagcgct
    14461 gatgaaattt gatggtattc tgcacagcgt agaaactgac gaaaaggaaa agatcatctt
    14521 cgggctgcgc gcctgtgatg cgcatgccct gcgcctgctg gatcatgcgc tggtggatga
    14581 acaaaacccg gatgagtatt gggagaaaaa acgcgctgct gcggttgtca tcacgctggg
    14641 ctgcaactct ccagacgaaa cctgcttctg caccgcggtt ggcggttctc ctttcaacac
    14701 cgccggctca gatgcgatgc tggttgagat gggggaggag tatctggtat acagcatcag
    14761 caaaaaagcg gaagccttgt ttcaagatct gccgcaggcg gatgggtcga aaacgcagaa
    14821 cgctcggaag ttcatgaaag gggttgaaac cagaatgccg ggcttgtttc ccaaacagga
    14881 gctgcatgat gcgctcatgg cgagctttga aaccgatttt tggaaagctg tttcgcaatc
    14941 ctgtttgggt tgcggtgtct gcacgttctt gtgcccatcc tgcttttgct ttgatatcgt
    15001 ggatgagacg gtgagaagag agcgcgtgcg caattgggat agctgcatgt tccgcaaata
    15061 ttctctggaa gcgtcagggc acaatccacg ccctacgaaa atagaacgca ccaggcagcg
    15121 tgtgatgcat aaattcgcct attgggtgga tttgatcgat gaaatgggat gcacgggatg
    15181 cggacgttgc gtgaggtatt gcccggtcaa tattgatatc cgtgcgattg cgacagaagc
    15241 gctccatcga gaatttgagg gaatgaagag atgaagaatt gctatgaacc cattccgatg
    15301 catattgagc gggtggtgat agaaaccagc gatgcaagcc tgaagtcgtt cgaattgact
    15361 tttaatcgaa aaggggactg tgaagaattc ttttcgcaat accgtccggg tcaattctgc
    15421 cagctctcca tctttggaaa aggggaagcc cccatcggca tcgcctcggc tgcctgggag
    15481 ggtgactttg tgcgcttcac cgttcagaaa atgggcaaat tcacctccgt tatccacgaa
    15541 atgcacgcag gcgatgcgat tggcatgcgt ggtccgttag gaaatgggtt tcccttggaa
    15601 gaatggaaag gtatgaagtt attgatcgtc ggcggtggtt gtgcgttttc caccttgtat
    15661 gccatgacca aacatatctt gcatcctgca caccgctctg attatgccga tataaccgcc
    15721 ctgtatggaa ctcggaattc cgggttatgc ctgtataagc cggtcatcga agaatggcac
    15781 aatcgcaacg atattgttgt gcatcaagct gttgacgtgg ctgaggaaga ttggaagtat
    15841 catgtgggtt atgtgccggc agtgctggaa gagctggctc ccaaaccgga taacaccgtg
    15901 gcggtgctgt gtggtcctcc tgtgatgacc aaattcacca tacccgtgct gaccaaactc
    15961 ggctttccgc cggagatcat ttacacctcg ttggagaaac gcatgaagtg cggagtgggg
    16021 cattgtggac gctgcaatat cggtcataaa tatgtgtgca aagatggacc ggtgtttaca
    16081 ttgcaagaac tgcaaggact gccgaatgaa atataggaga gccggatgtc tactcgtgga
    16141 ttgacgcaaa agacacgaac ggaaagtgag ctggtgctgg aaagatccat gatcacccgc
    16201 cattatgtgc tgcgctggga tctcgagcgt tgcgtaggtt gccagctagg accgctggtt
    16261 tgtcctaaag aggcgctgat ccatgtggat ggcgttattg agaatggacg aatggtgcaa
    16321 aaacaaagcg tcgatgtcga tccgcaaaag tgcatcctgt gtggggaatg tgtggaagtt
    16381 tgtccggtca atgccatcga aatgctgatc aatggacaac ccgagaaccc ggtaattgaa
    16441 tatgaagctt ttcccaaatt tgagcagagc accacgttcg atgcttctgt tttcgattgg
    16501 aagaaaaagg attttgtgat cgataattgc cccaccgctg tgatccgtga gaacatccaa
    16561 gagcacacgc tcgaagtgga cgatgctcac tgcatccgct gccgccagtg cgagatagcc
    16621 agcgatggcg cgtttcgcgt gctgcaaccc tggcagggga gtgtcgtcct acagagggaa
    16681 ctgtgtgttg cggactgcct ggcttgcgcc gatatttgcc caactcgcgc actacgcgtc
    16741 aatgaagcgg gggagctggt attggcagat tacttttgca ttaaatgcgg tgcctgtgtg
    16801 cagatatgcc cggtggaagc acaatatgag gaatatgaag tcaaagtcga atcgcagggt
    16861 gttaccaaag tggtgaagca tcagcgggtg acgaattcca aagccctgcc catctgggtg
    16921 gaacgctggc gtgtgcacca tcaggcggtg aaaagtggag cctgggtgca ggcgttgttg
    16981 aagttggcag atgaaaaagc gggaatggtc gaaatcgatc gtaaacgcgc cttgaaacgc
    17041 cgtgatctga ttctggcgtt gaaaggtgga aatcaagaga aaggataatt ccgacaaaaa
    17101 gctccgccag gggctttttc tatccttctg tgtcttgcgt ttggggaagg tgggtggctg
    17161 cgccccaggc gatgaatccc aatcctattg cagaaaagaa accgccggtt aatgtgaaac
    17221 tgtcgccgat gtgttctgtg aacagtgcct gcagcgtctt cagaatctcc tgcgatgaga
    17281 atggcagcat ggctgttgtg agcacataaa attgtctcat tccattttcg agcaaattcc
    17341 gcatcagggt ggtgtttaca gtcaaaccaa tgatgagcac agccagcgca ggaaggatca
    17401 gcatccaccc caaccataac agacggtccc tgccattgct tccaacgagc aatgcggtca
    17461 ggatccaaag aaggagggtg attcctgcaa agatgtatag ggttgttcga aaacttgcca
    17521 ggttcaaata ggggtgaagg aatgaaggaa tgttctgggt cagatccgct ttggtgaccg
    17581 gaccgaatat ggagatgcta ttttgcataa aagccagaag taccggcaga ttattcttga
    17641 ggaattgctc tacgaaaact ttctcgctga tgtcggcagg tttgcataag gctgccaata
    17701 atccttgcgc agcctgggtg ttctggcaag agggaagcac ttttgccagt tcttcgagca
    17761 atgcctgctg gtttggtccg ttaaaggcag ctttgacctc gctcaagtcg atctgcaggg
    17821 tcaatgtttc gcttttccct tccatgaaat cgaaaacttg atccaccatg cctgtgattt
    17881 gatctgtgta ggactgttcg ggaaccagtg acctcactaa ggcgaccgct gcaaccgtgt
    17941 cgattccttc gatggcaccc agtttctctg tcagcatctt cgcgatcgct tcatcggaga
    18001 aaagctttgc ttttacctct tcagaggtga acaattcgcg atagaggttc cgatcgaacg
    18061 cccagctcga aatggagtaa atcatgaggg tggaaaagat cagcggcagg accagaaaaa
    18121 ttataaaaag gacgcccaga agttttttca ttttcttaaa cccgtgtttt tgttttccat
    18181 tataaaacag gagcgatgat cgtgttcctc ataacaaaaa gagctgattt cagaaaatca
    18241 gctcttcgaa gaattccgat cagatcgtac tatcggcttt tttccacgat gtccatcaat
    18301 gcgcgcactt tatcgcggtc gaacggattg gatgctttct tatcctttcg caggtgcgtg
    18361 gccaccacag caccgtcgca gtgtttcagc atttcagcga tgttttccgg agtggcaccg
    18421 ttattcatga tcaccgggca ggtcttgctt gcttctttta cgatctttaa ttcatcaata
    18481 gtcggtgatt ctcctggaat tggtccgcat aaggcaaacg catcgggttt tgaggaccac
    18541 atggcgtctt taacgtcagc ttgcaaactg cggtcggtga tcgacttgca cagaatgggg
    18601 cgcagcatga acatcagttt cacgtgttct gcgtcgatct ggctgcggta tcgcagcgca
    18661 tcagcggcgt tcaggctcat caaacccatt tcccctgcat aagcccctcg gaacacgcca
    18721 cgcacaaact ttgcgcccac agctttggca atggcgaggg ctgccaggtc atcccaaaag
    18781 atggatatgc cgatgggtat ctgcattcct tgtgatgctt caccgaggat cttgctgatc
    18841 aaggctgggg tttcaggacc cactcctacc cggtatggcc aatcttgctg gttgccgatg
    18901 ttcacgccat ccattccgcc ctcttgcagg gctttcagtt ccgcgtgggt gtaatcgatg
    18961 atctgttgca gagaggtcac tttttgataa tctgggctgc ccggcagggg gggcagatac
    19021 atggatgcga tcattacctt ttctttatgg aatacttcat tgaaccaggt cataagttcc
    19081 ttttcgagaa atagaaatct cccctctcag ggggagattt cacagattga ttatttgtcg
    19141 atcgagaaat agcgcgcagg attgttgatg tagatatctt cgatagcgtc ttcgggcacc
    19201 ccttcttcgc gcaaacgggg gaggaaaacg gtctcgtcat agtcgatgcc ggttccactg
    19261 ccccaactct tttggtacgt gcgccgaccg gcatcggtgc cgaggcaaat atgtttctga
    19321 taaccggctt tgaccaactc ttttatgagc atggcgcggg aactgtcggg caagtatttg
    19381 atgcggtagg aaccgtcata catgaaattg gctcccatgg ctgcgatctt cttgtggtac
    19441 cacacatcgg ggttgcgctg gacatgcccg atgacgatct tctcaggttt cacgccataa
    19501 ccgatcaggc gttctgcctg ctcataagcc atcgttccac ccgaagtgtg ggtgttgatg
    19561 catacgccgg tttcttcttg agcgatggca gcggctttga tgattcgttc ttcaactggg
    19621 gtgatcaaac cataagcggt accgatcttg atgcaactgg ctttggctga acttctttcc
    19681 acataaggac cattgtagtc atagatgtcg atgccttctt cgatatcggc gatcatcaga
    19741 tctgcgatac ggttaacctc gtatgtggca gtccagtgga ttacgttgtc atagatgcgc
    19801 ccctcatgaa aacctgtgca ggtcatgatg tgcacctcgg gaactgcttt ggcgatctcc
    19861 accagcattc tgatatcgcg cccattgctg atggggttca tatcaacaac ggtcttgccg
    19921 ccggctttgg cgaacaactt cacctcttcg atcgcctttg cgacgtcatt gagccgccag
    19981 tcttcgccgt tctttagaat ttccagaccg ccggtgcgga tgagatgctc gtgtgcatcc
    20041 acgatcccca tgtctttgga atcaatatct cccagaactg ttcttacttt tcccataggt
    20101 ttctcctttg cgttacggat aatttttcag gatgctggat atcgttgatg acgataaccg
    20161 cgacctgata tgttgtgtat ttttgttgcc ggaagtgtct tttgcaatct tgacaacaat
    20221 atgaatatat tatactatat gcacatatta ttatagactg caaatatttg caatttatta
    20281 gaaaagagct tgttgcgaaa tggtgtcata ataagaaaaa atccccggag agatgatgaa
    20341 aacaataaaa tttttagcaa tatgtggtaa cggacttgga agtagtttga tcgtcaaaat
    20401 gatcctcgaa ggagtattgg ctgacctgaa cgtgcgcggg attgtggaat caaccagcgt
    20461 tgcgcaggct gccggaatga tgccttttgc ggatgtggtc attaccagta ctgcctttta
    20521 caaagggata gaaagtgcta ttcccgaagg caaaccggtc attacctgca tgaacattct
    20581 cgataaagaa gagttgtcgc agagagtagc ggaagttgta aaagaaaaat tctccagtta
    20641 aatccttgaa aacaaaggag gtgcagctta aagaaatttt tgaattttat tggtgatgac
    20701 gtattactaa aataaaagga gatttgaaat gcaagtactt ctggatattt ggaatgtaat
    20761 tttcaaccag gttgttacca agccgcaatt gctgctggct atcattgtcg cgattggtta
    20821 caccttatta aaaagacctt ttatcaatgt tctttctggc gcgatcaaaa ccatggtggg
    20881 cgtgatgatt ctgcaggttg gatctgggca actgaccaac accttccgcc ccattctgct
    20941 tgccctcggt gagcgcttcg gtatcaccgg aaccattatc gatccttaca ccggtttccc
    21001 tgccgctctg gaagctctgg gcgataacca gtcttgggtt ggatatgtga tcttgatcgc
    21061 ccttggtgtc aacatccttt tggtagctgt caccaagatg cgtggcttgt tcctgaccgg
    21121 tcacatcatg ttccagcaat cttgtgttgc cactgccctg atcgcctaca ccctgggcct
    21181 tcccatgctc cccaccattc tcatcaccgg cgccctggtt ggtctttact gggcaattgg
    21241 ctccaacgcc ttgattggtc caaccaataa agttaccaac aatgccggct tcaccatcgg
    21301 tcaccaacag atgttgatgg actggattgc ttcgaagttt tcccacaaat ttggcgatcc
    21361 tgagaaagac gattgtgagc gggtagagtt acccggatgg ttcgctatct tgcaagacaa
    21421 cattgtcgct atggcaatca tcatgttctt gttcatcctg gtcatcatgt tggctttggg
    21481 aaaggaaact gttgatggaa tggctgctgg caccaactgg attctccata ttctcctgac
    21541 cggtgtttca ttcgcggtct atatcaccat cattctgacc ggcgtccgcc tctttgttgc
    21601 ggaattagca acctcattta aaggtatttc tgaaaagtta ttgaagaatg cagtgatcgg
    21661 cgtagattgt cccgcaatta tgcccttctc acccaatgcc tggatgatcg gtttcctctt
    21721 cacaaccctg ggtgaattga tcgccatcgg cgtcctgatt ctgatgaaat cacccgttct
    21781 catcatcgcc gggtttgttc cgctcttctt tgatggcggt cccattggcg tgtacgctaa
    21841 caagcatggt ggtttcaagg ctgtggcttt cttctgcacc ctgaccggtc ttgtacaggt
    21901 ctttggctca gcattgattg ttccaatgtc tggtatggtt ggcggttaca tgggcaactt
    21961 tgactgggcg accgtctggc cggctatcat tgtcgtgctt cgcttcatcg gtaatctgtt
    22021 cggattgcca gtgcctccct acggagcata gtgttcgttt ggggtagcgc tgaaatagct
    22081 tagtatttca gcatattgaa agggagctcc atgattttga tcaccttgtt ccatggagcc
    22141 ccttttttct tcaagtgcct acacccctca agaaaacagg aatgattcaa atggcagcga
    22201 cattattgga acttctgcaa gaaaacatga tcgaattaaa tgttcccgtt gggaattggg
    22261 aagaagcctt gcaacaaggc gctcaactcc tcatcgacag cggcggtgta gaaccacggt
    22321 atttagatgc gatgattgct atggtgaagg atctcggacc ctacgtggtt atcgcgcccg
    22381 ggctggcatt gggacacgcc gggcccgatc agggtgtgaa gcatacctgc tttagtctgg
    22441 tgaccttggg cactcctgtg aattttggcg tccctgaaaa cgaccctgtc gatattgttt
    22501 ttagtttcgc tgcgccgaac aaagaagaac atatgcaagc gctgcgcgat ctggcactct
    22561 tttgcagcga aagggagaac ctggacgcga ttcgcgcagc gaccgataaa aagcaggttc
    22621 gagaagtgtt gataaaattc tttcattcat aatagaataa acttgaaccc gggaggagga
    22681 aatttgcggt tgaattcaaa agagatgaat atgatccgcg cagccgaatt gtattatgaa
    22741 cagcggctgg ggcaatcgga gatcgcaaaa attttgaatt gttcgcgttc taccgtttct
    22801 cggctgcttg ctgaggcagt cgaatcgggc attgtgcgaa tatacatcaa gcgaccagtg
    22861 gagaaatttc cttctctggc agagaaggtc aagcatcatt ttaaactcaa agatgcgatt
    22921 gttgtttcag gcggtaccag caatgatcag gctttttaca atgtgggctt tgcagccgct
    22981 gagttcttat cccagattct gcatgatcat gttgtcattg gcatcagttt cggcgttacg
    23041 ctttcttata tggtgcagga attgttcgaa tatacctttg aatacgatgg catcgaggta
    23101 atccagttga tgggtggatt gggttttggt gacccagcca ttgacggacc ggaactggca
    23161 caaaagatgg ctcaggcatt gggtggcaca tatcgctatc tgcaggctcc ggcggtggta
    23221 gattctccag aaattgcgca gcatttgtta aatgaaaaaa cgatacgaga aacgatacaa
    23281 aaggctttcc aggcggagat cacaatttcc agcgtgggtt cattgatgga taatctttcc
    23341 agccttgaac gatctggata tattcgcaaa gaggaacgtg ccacgtttca agccaaaggc
    23401 gccatcgggc attcactatc gagaatgatc gatgaaaatg gcattcctat ccatgacccg
    23461 ttcaatgcgc gcatcgttgg agcgccgctg gatgtgatca aaaatgcgaa atggtccatc
    23521 gggatcgggt cgaacccctt gaaaacacaa gccttcttgg ctgcggtgaa agatcgccaa
    23581 ttcaatgttt tggtcatcga tgatggcaca gcccgagaaa tgctgcgcct gcaggctgga
    23641 acttctgaat agatctaggc tcactcggtt gcaataaaaa tacctttttg aaaagataac
    23701 cctgtggttg acggggcatg atcgattcag gatgtatcaa taattctgga aacgcactgt
    23761 tcaaaaacaa cataatagtg ggggtgcgct gttttgattg caacgaaaat ctccgaaatc
    23821 cgtatatcct gtatatacta tggatgagac cgtataggag aaaaccatgt ctgaaatacg
    23881 aaaagaaacc agagagttgg gcgaattcca tgcaatcacg gtaaagggta ttggaaacat
    23941 cgaattgaag cagggatccg tgcaacaggt aacggtggaa gcggatgaac atcttttgag
    24001 caggatcacg accgatgtga tcaatggctg cctgatcatc gatattggtc gcgattggct
    24061 tgaaagactt tcagccggct tggattttct cgccgcccgc tcgatcaatt tctatatcac
    24121 catgcagcaa ctggataaat tggaggtggc tggcagttgc aagatcgaag ttaccgggtt
    24181 caaagggaaa gaactgcggt tggaattgaa cggagcttcc aatatggaac tggctgacat
    24241 tcagtatgaa gcggtgaata caagtctggc tggggcagga aagctcgatc tgcggggaaa
    24301 gacgaaggag caggctgtca ccctggcagg tgcgggcagt tatgtggcgg atgatcttga
    24361 aagtgaccgc acgcgcatcg ccctcaccgg cgctgggagc gctcggttgt gggtgacaaa
    24421 agagctggat gcgtccatca cgggcgttgg ttccatcgaa tactatggcg agcccgaggt
    24481 gaaacaaagc ctcgcgatga tgggttcgat caagaactta gggaagaaat agctcgcacg
    24541 cgctctgaaa taaagctcca caggacgacg tcggagcctt gtttttggct acggagaggg
    24601 gtgaatggat caattccttg actgcgtgtg tggctgggtg tacactagag actgtaagat
    24661 ttaaagaaag aaaaggaaaa atgcaagaag aaaaatcgta cgatctggat cctgacccta
    24721 aacgtaactt aacaacgttt tcgtgcgaaa tagcattgtc tggaggtgtg ttgctgtaaa
    24781 aggcacccac aacagctctt gattgttttg tctgtatggt gacgaactct cagctatttc
    24841 gcatggaaag gctgggagtt ttttgtttgg atcggggata atacgctgag gaggtatccg
    24901 atgatcacaa ttttacagac aaaaaatgaa acgcttgtcc cgatcgagca atttgcggat
    24961 ggcgcatgga tgaatgttat taatccggac gagcgtgata tcgaacgctt actctccctt
    25021 ttgttggaag atgtgatcat tgagaaccag caagctcttg agatgacggg aattgccggt
    25081 aacttgcttg ggcaaatgat ggatgcttat gcgtccatca tccagaacaa tcaaaacatc
    25141 attttcaaga tcatgacagc ctgggccatc attttgagct tgcccgccat aatctcggga
    25201 ttctttggca tgaacgttgg tttaccgttc gaaagtcacc ccaatgcttt tatgatcaca
    25261 acgggcattt tgctggctat catgctggtt gtaatgtttt ctttcggaag atcaattggt
    25321 tatgaaaatg gatcggtgag aagaggtaag aagatgagca ggtcaatcct taccaattcc
    25381 atccagagca agagaattct gggtagaagg gagatggaaa gatgttctat ctgagtgaaa
    25441 tactggacaa aaaagtgtgg gatcgctggg gtaaggagat tggcaaatgt cgcgatatcc
    25501 tgactggctt tgatgagaag gtctttcatc gcatcgtggc tgtggaaatc aaagagaaag
    25561 atggaaatac gcggttgatc tcatctgagt cagtcagcag ccttttcccc aatgtcacgg
    25621 tcagcaaacc gatgatagaa gtgaaccctt atactcccaa ggggaatgaa ctgtatttgc
    25681 gcaagcaagt tctggaccac cagatcgttg atgttgaggg aaaacgggtg gtgcgcgtca
    25741 acgacatcca gattgccttc acgcaaaatg cctactttgt gaccggtgtg gatgtgggaa
    25801 atgccggctt attgcggcgt ttagggttga aaaaaatgaa cgaactgttt aaaaagctgg
    25861 caaagagttc ttcgcccgaa ggggtcatat cgtggaagga tgttgcctac gttgaggcga
    25921 aagacccgct gcgcttgaaa acatcacagg aaaagatcag caaactgccc cccgctgata
    25981 tcgccagcat tctcaatgac cttgatcgac caaccggtca atcactgttg cagcgcatgg
    26041 gtgacgaaat actggcagat acgttggagg aaagcccggc aaaattccag atcgatgcgt
    26101 tatctcgcat ggcttcagag cgagcagcag acattcttga ggagatggag ccagatgaag
    26161 cggctgacct gattgccagt ttgccacaag agcagggaag cgtcttgctt gacctgatgg
    26221 aaaaagaaga tgcggacgat atccgcactt tgcttgatta cccgccagac tctgccggtg
    26281 gaattatgac cactgaattc gcctggattc cccgcggatt gacggttgaa aaagccatgg
    26341 ctttcttacg ttcatcagaa gacgcccaag aagtggaaga tatgtattac atttacgtcc
    26401 tggatgaaaa aggaaagctg gcaggcacgg tctatttacg cgatctggtg atggcggctc
    26461 ccactatgga agtggaatcg atgatggaaa aagattcaat cgtcgtctcc ccattagcta
    26521 cccaagaaga ggcagcatac ctggttgcga aatataacct gttatcaatt ccggttgtgg
    26581 atggtgaaac tggcatcatg cagggcattg tgaccctgga tgacgctctg gacgcagtcc
    26641 ttccgacggc atacaaaaaa cgcttgcccc gtttcttcta ggatgtgcat atgaaaaaag
    26701 cgacttcaaa ctctacattt ttccgaaaat tgtggctctt gttgaccata ctcggtccgg
    26761 ggtttatcac ggcttctgcg gataacgacg cgccagggat tgcaacgtat tccatggcag
    26821 ggtcgcgcta cgggtacaat tttctctggg tgctggtggc ggttaccttt ggcgaagtga
    26881 ttgttcaaga aatggcagct cgcatgggta ctgtgacggg gaaggggatg gctgatttaa
    26941 ttcgtgagaa gttcggcgtg agaactgccc tgtttgccat gacctgtttg ctggtagcaa
    27001 atataggaac taccattgcg caattcgctg gcgttgcagc cggtgcggaa ctgcttggca
    27061 tcagccggta catcgcaata cccgttgtcg ggatcggtct atcgctgatg atcatgaggg
    27121 gttcttataa ggttgttgaa aagatcttga tcatcctttg tatggctgct ttgagttatg
    27181 tagtcgccgc catcgctctg aagccagatt ggaatgagat cgccaggtct gcttttatcc
    27241 ccaagatcgt ttcattggat agcgcctatt tgttggctct gttagccaca attggcacaa
    27301 ccatcacccc ctggggtatt ttttacatgc aagccgcagt ggttgataaa ggggtgaaag
    27361 tggaagaata tcccatgacc aaagtcgacg tggtatttgg ggcagtgtgg gggaatatca
    27421 tttccgcgtt catcattatc acgactgccg ccaccttatt taagaatggg attgtagtgg
    27481 aagaagcaga acaagcagcc ctggcactcg ctccgctggc tggagaatgg gcgaaagtat
    27541 tattttccat tggattcatt ggcgcttcca ttctggctgc tacggttctt cccctttcca
    27601 ctgtttaccc gatcgcagaa gcttttggct gggagcgtgg gttggataat agccgcaagg
    27661 atgctcccat tttttatggg ctgctggcag gcgtgatcat tgtcagcgcg atcgtggtgt
    27721 tgttccccaa tctgccgctg ttccaattaa tgtggacttc acaagccctc aacgcagtcc
    27781 tgttaccggt aatcttgatt cttatgttga agctggctaa caacgaagaa cttctgggga
    27841 aatatgtgaa caagaaatgg cagaattatt tttcttattc cttaatggtg atcatttgtg
    27901 tagccactct ggtgcttttc atagacccat ggattgctgg atgaatgcct gacaaactac
    27961 aaacgtatcc cgttagcaaa gaacagttca aaatcgggat acgtatttaa ttccagatgt
    28021 tcgatctttt cagaaagcgc tttggctttt tggcgcattt tttccgagag tagcatcaat
    28081 ctggctcctg cgccggcagc gttcccgact gctttgatct tgctgagctt cagcaccggg
    28141 agcatgccaa tttggatggc gtgatagggg ttgaggtacg agccgaaagc accggcgatg
    28201 agaacttctt ctatctcctc tgcttgcacg ccaagtttat ccatcaagat ggcgatcccg
    28261 ctgcggatgg cgccttttgc cagcaggatc tggtcaatat ctttttgcga gagcggtatt
    28321 tcgcgctgcg attcacttgc cggcaagagg ggatagtacc ggacgccatc ctcgttgata
    28381 gcaacatggg aatgaccggt gtcaagccga ccgcgttggt tcaagatgcc aacccttttc
    28441 atttctgaaa tggcgtccaa tatgccggaa ccgcagattc ccacggcttc gccctcgccg
    28501 atcaccgcac actttgcctg tccatcccgt tttatggaaa catgttcgat cgctcctgga
    28561 gaggcgcgca ttccgtagcg gatgtgcgct ccttcaaatg ctggtcccga cgctgtagaa
    28621 caactgacga ttctttttcc cttttgaagg gcgatctcgg tgttggtgcc gatatctatt
    28681 ccaagccgga tcgtttcatc ctctccgaat ccggcagcca gcagaaatgc cagatggtct
    28741 gaaccgacga aaccagcaat gggggcggga acgaacacgc ggctgccggg cataccggac
    28801 actccgagca tttcagaaga gaggtacatt tctttcgtca gggctggggt aaagggagag
    28861 gttgccagat atttggatgg cagatgaaga aaaaaatgat gcatggctgt gtttccaaca
    28921 acagccatat ctgcgatctc atcaattcgt gatgaagaga tcgcagtaat attttcaata
    28981 gcctggttga tcgcggaaat gagtgcagtg tgcaactgcg cttcttgttc tgtgccctgc
    29041 atggcatagc tcaggcgggc aatgatatcc tcgccatacg cgatctgggg gttggggatg
    29101 ccttttactg ccagcgtgcc gcctgaaaca agatccatca aataacaggc gatcttggtg
    29161 gagccgatat caatagccaa tcccagcacc tgccggggtt tttctggttt gacatggata
    29221 attgtatcct cgttgcacat ggcgtatact ttccagccat tctttctgag ctggtgggga
    29281 agtgttcttg ctatttccag cgctttttca tcgttctcaa agtgcagggt ttttgaggct
    29341 gccagaacat tttcaagatc ggagcgaggc gcaatgagag aaggaggggt tagctgcagc
    29401 gcttcaacgt ggataggcga ttggacgtgc tcgctttgaa actccccttc ggtttgcaga
    29461 acttgagctg tgctgagaga ggtggttggg atgaagactt ttgcattccc gtcaaggata
    29521 gcttcgcaag ccaatcgctc atctgacaac agaaccccac ctttacgaaa tgtctcctca
    29581 ctttttgtta agggattgct gttgccttcc atgatccgaa tgacacattt tccgcacgaa
    29641 ccctttccac cgcaatcact ccgcagttgg atacctgcct gtctggcagc gtccagaatc
    29701 gtgactggct ctgagaaagc caggcggatg ccgatcggtt gaaactcaat ggaaaaagag
    29761 gattctttca tgaaaatgtt cgaagggtgg gtagggtcaa ccaaccgtgc ctagatttca
    29821 atctcgctga tcgatttata gatgtggttg ggtctgtagg ggaaattttc gatatcttta
    29881 agggtcgtca cgcctgatag caccagaatg gtctccatgc cgctctgcaa accagcgaca
    29941 atatcggtgt ccatgcggtc accgatcatc accgtgtttt cggagtggac acccagatgg
    30001 ttgagagcgg aacgcatcat gaacgggttg ggttttccgc agaaaaacgg gcttttcccg
    30061 ctggcttttt caatgagggt cgccatggca ccacaagcag gaacaattcc ttcatcgcta
    30121 ggaccggttg ggtcagggtt ggttgccaga aaatgcgccc caagtaggat caagcgaatg
    30181 gctttggtga cctgccgaaa attgtaatcg aaggtttctc ccaaaacgac gaaatctggg
    30241 ttttcgggag taagaatgaa acccgcctcg tgaacggcaa ggtttaaacc gctttcgccg
    30301 atcacaaagg cgtttcctcc tgccttttgc gttttcataa aatttgccgt tgccatggca
    30361 gaggtgtaaa tctccctctc gttcacatcc aatcctgatg tttgcaaacg atgcgccaga
    30421 tcaatgggtg tataaatgct gttgttggta aggatcaaaa acttgcagtt cttatcgcgc
    30481 aatgtttgaa tgaatttgtc ggctccggga atcatcttct tcccgtacac caacacacca
    30541 tccatatcga tcaagtaact tttgttttcc attatatttc ctgccttaag aaagtttgat
    30601 ctcttgagat tgtatcattg aaaaagggac gcatgctgga aaaccaacat ctgcgtccct
    30661 tctcatgaca gaaaaaacag cgaagttatt tgctgaccag ttttctgacc tcatccagct
    30721 ttccggcact gcccaatttt tcattcttgc tggcaatgaa tttggcgtat tcatccatga
    30781 aaactttgcc gggcttgcgg aagtcgaaca cttcggggtg ctcgatgaag tattcacgat
    30841 gcacgcgtgt ccataccaga cgaccatcgg tatcgatgtt gatcttggtc acacccaatt
    30901 ccgcggcgcg tttgaattgg tcggcgtcca cacctttcgc gccctgcagt ttgccgccgg
    30961 ttttgttaat gcgctccact tcgtcttgtg gcacggaact gctgccatgc atgaccagtg
    31021 ggaaccccgg caattgtttt tggatctctg ccagcacatc gaagtgcagc gattggctgc
    31081 ctgaaaattt aaaagcgcca tggctggtgc caatagcaac tgccagcgaa tcgcagccgg
    31141 tgcgatcaac aaactctctg gcttgggcag ggttggtcaa tttggcttct tcttcgctaa
    31201 cctgcacatg ctcttccacg ccacccagtt gaccaagttc cgcttctacc accaatcctt
    31261 tggcatgggc tgtttcgacg acccgtttgg tggtggcgat gttctcctca aagtttaggt
    31321 ggctcgcgtc gatcatcaca gaactgtaga aaccgctgtt gatgcaatcg tagcaggttt
    31381 cttcatctcc atggtcgagg tgtacggcaa acaccgcttc cggaaaaacc ttgtcggctg
    31441 ccacgataat ggattgcagc atctctttat cggtataatc ccgcgatccc ttgctgatct
    31501 ggatgatgaa tggggcttta ctcatcaaat tccctcggaa caaacccatg gtttgttcaa
    31561 ggttgttgat gttataggcg ccaatggcgt acttgccata agcggcttca aataattttt
    31621 ttgttgtgac aatcatccta tactccctgg tacatttgtg atttacaaaa tcactgtatt
    31681 atatcgcgat ttcaatttgg tgaaattcca ggggaaataa attttgtgta attatttact
    31741 cttccacttc atgcggtctt ctttatccag caagcgcgct tcctgaaata ccgtgatcat
    31801 gaagatgaca tataataaaa ccactaccgt gccaacgatg agattataga gcccgacctc
    31861 tccaactcca aaaggcaatt tgatggcggg gtttcctatg aagaagataa aagtgtttgc
    31921 gaggattgcg atggctctaa tttctgtggg acccagtttt ccgtatgaaa ttttgaatac
    31981 cccgcgcagg ttggtggtaa ggtatacctg aatggtcata cacaaataac cgatcaagcc
    32041 cataaccgcc aggttgaaat caacatactg cgataaacca agaccgagga aaatggtaat
    32101 ctcgctgatg gtatcgacaa tatggtcaac gtaaaaacca tacttgggac gttcaatttt
    32161 tcggtaccgc gccagattgc catccaggct atcgccaacc caattaagga tgaatccgaa
    32221 actcgccagc cataaataat tgggactgta gcgggttgcc caataactca cgccaatcac
    32281 caacgccgca atcaatccaa tgaccgtcag atgatcagga gtgacccagg cgggcatatg
    32341 ttccaccagc cactggatgg ctggtctttc aagaggtcct agcaagatat cattcacacg
    32401 tttgtgttca gaaatgtcat gatccggttt ttgctcgttc atactgcttc ctttccaatc
    32461 aatttgcgta aaataatttt actggggcat ttcgtccagt gcccaaggcc agacgggata
    32521 gaacattgac cattgctcag gatgagcgag aatgaatgtc tctgcttgtt ttaacacttt
    32581 ttcggcgttt cgttccatct cttcgacggg gtcgctatat ctttctaaat agataaggtc
    32641 agacgattca gccgtatagc gaccatcttc ctgccccacg caagccacaa ccacgatcgg
    32701 ggagtgggtt tggagggcaa gtttgacgta agtgacgggc agcatggaag ggtatccaaa
    32761 gaacctggga tgatatttgc tttcactgag agggcgatct agccctgtga tgatggcatg
    32821 tccttcttgt aagcgctgtt tgcccaggcg cagtgattcc accgacatcg gcatgatttc
    32881 aatcgcatac tctttgcgaa gcttgttctg catctggtat cctttgttcg ggttggggta
    32941 ggaaaggacg aggatgtggt gccctttgta ggtgataacc cgccccgcca ggtcgaaatt
    33001 gctcaaatga ggggtcaaaa agatggtccc ttcttgatat ttttctctgc ttgccaagat
    33061 ctcttccatt ttcgggctga tggtgatctt tttgagcaaa gtttcagggg agtcaatgtt
    33121 gtggtacaga tcaaagaggc aatatgccgt atggtcaaaa acttgtaaaa cacgttggtc
    33181 aagctcctga ctggttaatt ttttcccgct gatcacccat tgattggcgc gaacacagcg
    33241 caccatcgag gcgtttttcc tttggctgat cacctttgcc agaaaatgcg caaaagggaa
    33301 tcctattttt tcaggcaggc gctttgcgag acctttcgca aaatttaccc ctatttcacc
    33361 attgatcaag tcttgaaagg acatagtgca ttaaatttaa catagaatta ttagattcgg
    33421 gtaagagaat atgcgcgcca accgtctcgg agagttttta tggcgaaagt caccgacttt
    33481 gtaaaagatg ctcttcaaaa atgctgatct gtttcgagcg gatggcagcc accttcaagg
    33541 gatcaaccgg gctgtgtgaa ttgctgggtg tgcgcacccc tctgccgatc tgcaggttgt
    33601 agatagtggt ttggcgcaag aaagtctcca cattcctggc ggtaatcccg ccggtcagga
    33661 ggatctcaat tctgtctttt gccgtttctt ttatcagaga aatgttttgc aagtttttgg
    33721 tgaaatctcc attcgtcagc gaattcgtca ggcaattccc actggtgcgc atggagtgga
    33781 atcccaggct gatggtcgtc tcaacggttt tgcgaatgtc tctggttaat tcccagataa
    33841 cgcggctgtg aagatggatt ccactatgtg ccgctttgat cgtttcaatg actgcaacgt
    33901 tcaggttgct ggattggtca atgaatccta aaagaaaatc gttgatccca agctttttaa
    33961 acgcatcgat atcccgcagg cagatttcca ggtcttcgtg cgatggaatg taaccacggg
    34021 tgtgaggaca gatcaatacc acaagaggaa tttttacctt ctcacggatc ctggaaacca
    34081 ttccataaga aggggtaatc ccgctgcttg gatagtggga aagaatggca atgcgcgttg
    34141 cgccccctga ttcggctgca atcgcatctt caactgtatc agcaatgact tccaagcaag
    34201 gcatgaattc acctgacatt gtgtggaaag aattgttaaa aacggatgac ttgtttcgtt
    34261 gcctatcttc ccgccatgat tggcttctat tatctccctt tttcgcatct tgtcaaatga
    34321 aattattgat cagcggattg ttcggtataa tttcagcgtt caaaccacct tcacgaatgg
    34381 attctagaat ggacgattct ttgaactttc atgcatgcat ttttgacttt gacgggattt
    34441 tggttgattc tgaggttgtg ctggtaaagt tatggaagcg tatcgcaggc gaatatcact
    34501 atgattttag cgaacaaagc tttcaaaaat gtcttggaag aaccacagaa gacattaagc
    34561 aaatccagat ggatgaattc ggcagccaat accctttcga tgaaatttat cagaaagttc
    34621 acgatagttt ccaccagatg atcaccgggc atggtttgcc gttggtgccg ggggtgcttg
    34681 tattaaggca gttcctcgaa gatgctcaga taaaaatggc agttgccagt tctacctatc
    34741 gggcagaagt tttgtttcgc atggagaaag ccggactgac ggacctgttt tcaacggttc
    34801 tgggagggga tgacgtcttg caacccaaac cggcgcctga tattttcctt aaggcggctg
    34861 cggctttgca gatggaacct gctgattgcc tcgttctgga agattctcaa agtggggttc
    34921 tggcagccag agcagccggg atgcaggttt ttgttgttca aaaccttagt ccggtgacgg
    34981 aggtcatgcg cgaaaaagcg catgcgattt tcccttctca tgctgctctg cttcaatttt
    35041 taaaaggagc cggtgctgct tagctccctg gcaacaactt ttccagtagc tgctgggtct
    35101 ttgcagcttc cagcccgttg ctctctggct ctgctcttgt ttctatgcag tgaaagaagt
    35161 gttcaatttc tgggataaat ccgcgctccc tcaaatctgg tgtccaattt ccagctctgt
    35221 ctgatccgaa caattcctca tgctcagcat agcgcaccca taagttgcga aatgcatcca
    35281 catggatgga aagattgtcg gcgtgtatgc tcacgctttc ctgccatatt ccggcggtgt
    35341 gcgctgaaag taaagtgccc agaccgccgt tcggaagaag gacggtgctg atgcaactgt
    35401 gcagtgtttg gtcgcgcatc tgaaaagatg tctgcaatgg ttcaacttct ccacagaaat
    35461 agcgcagcag gtcgatctgg tggatgtagt cttccaaata gaattctgtc aggttcggat
    35521 agtgaggcgt tgtgcggtgt ttttggatca cgatcaattg gatctctttc ccatccagct
    35581 tttgcttagc ctggcggtag agcaatgcgt atcggcggtt gaaaccgacc atgaggatgc
    35641 gctttttctt ttgtgccagt tccgccaact ccaatgcctg ctggctggtc gtggtcagtg
    35701 gcttttctac aaaaacatcc accccatcct caagcagtgt ttgtatcaag gaataatggg
    35761 attccttgct ggtcagaaca aaagctgctt tgatgtttaa cttgaaaaga tcatctatac
    35821 tggtggacca atagggcagg ttccagcggg cacaggcgtt catggcgtgt tgcgcgtttc
    35881 tgctgtagat tgcagcgatt tcgatccctt cccaggcagt taacagcggg aagtatgctt
    35941 tctgcgcgat actcccgcta ccaaggatcg cgatgggagt tcggggagct gcttctgatt
    36001 tattgatcat ggataaaaac tctcttcccg tcttctcgtg cagattgata gcaggcgtgg
    36061 agaagttcct gcgctttcag tgccgtttgg aagtcggctt gcgaggggat ctgtttttcc
    36121 aggcagtcca gaaatgtgga aatcacggcg atgtgggcgc tcaagaaacg ccccagcgat
    36181 cgtttgtttt ctgaccatcg tgaccgatcg aaatgtctcg gcgtaatgcc ttcgggcaga
    36241 actgcgctag agatccattg gtcttttttg acatcataat attgtatcgt ttccgactgg
    36301 ttgaaatcga tctttatgga accgcggctc ccgaagattt ccaaacaggc gatgttcccg
    36361 gcacctcccg ccacccggct cacttccaaa gagccgatcc ctccattttg caactgcatt
    36421 gtgcatagtg cccaatcatc tacacacact tcctcgtatg aatttgggtc atcctgcctg
    36481 ggtcgtgatt tgataaacgt ctctgcctgg cactgcaacc attcgacatc ccccaacaga
    36541 ttgcggataa ggtcgagaaa atgaatgccg agatctgtca acgctcctcc gccggcgatg
    36601 tcttcctgca gtctccagga gatggggcgt tgcgggttga ggtaggaact gtgaaagagc
    36661 ctggattgga aatggtggat ctctcccagc caaccggctg cgatgagatc tttagccaga
    36721 cctactgccg gaagaaaacg gtaggtaaaa gcagtatgcg tgggtatttc ttgttctctc
    36781 accaattttg ccaattcgcg ggcatcttca atcgtctttg ttaaaggctt ttcgcaatag
    36841 atgggaatct tttttttgat ggctttcagg caatattcat aatgagacgc gtttggcgaa
    36901 caaatatcaa tcgcgtccag ctcctggctc cagaagagtt gttcatcgtt cgtgcagatc
    36961 ggaaccgcca gcgtttccag cagctcttta tcttttgaag gatcgttgcg cagcactgcc
    37021 atcacccgcg ccaatgctgg agggtctgca agacaatacg gcaggctacg atatgcctgg
    37081 gcatgcacct ttccgataaa accgaatccg atcaatccga tcttgtaggt tttcatgatc
    37141 atttccctgt tctttgttgc gtgctgtgat attctttctt tgcgatggtt ctaaagccag
    37201 cttgattata ctggagagaa gcgctactgg agcaaaccgg gcaaagctgg ttggacggca
    37261 ggtcttttcc cgttcatggt gatgaacggc aacccttttt gaatttggat accgattttg
    37321 gcgagatcag attcggtgcg gaaaggatgg atcttgcgca tctggatgat cttgcctgcc
    37381 cctttttcgc caatgcctgg gatgcgcatc aatctggctg gttcagcctg gttgatctca
    37441 ataggatttt cacgaaggtt catctcagcc cataataatt ttggatcctt ctccagcggc
    37501 agattggatt tgtgatcgaa ataaaaatca tccaggctgt aaccataatc tctgatgagg
    37561 aaagaggctt ggtataaacg aaatttcctt ttcaggggta tgggggcgtg tgattccagt
    37621 ggagtacctt tgtacggtgt aaggggcgaa aagtaggtcc tttgtaagtg aagctcagtg
    37681 tagagataac aggtggtctg cagcaattcc ttgtccattt cttcggaacc ccccactaca
    37741 aattgcgtgc acactgaagg ccagtgacct ttccagttgg cagaggttgc ctcattttgc
    37801 ctgaccgtat ttgtccattt caacggatcg attaactgat cgaaaggggt tttgtcagga
    37861 gagaggtatc ggatgcgttc tgcgttgggg gcttccaggt tcagggaaac ccgatcggca
    37921 agatacatga tgtgttttgc ttgttcaaat tcgagacccg gcatgaattt aacatgcagg
    37981 tagccccgaa aatccatttt caagcgcaaa atttccgtga ctttgatcaa taaatcctgt
    38041 gttcgcacgg agttctcctg aatggctgaa ctgataaaag ctccgtcgat gagcccggcg
    38101 tggtagagat tgttgatcaa tcgcgcatac tcttccgcct gaaacgcagc ccgcggctga
    38161 tcgttatctt tccgtatagg acagtaatag caattgttct tgcagttgga ggtgatcaac
    38221 gacttgaata aacgtatctt tttcccattg ggcagggtgg catgcgacac gcagacatct
    38281 tggatagcgt tggattttcg gaactgacag tgttcttcat ccatttcgtg cacattgtgc
    38341 aaggtgagca attgcagctt ttcttcggtt ttcatgcttc tattatagaa caaaaattct
    38401 atttcatcta tactgataat caaagcaaaa cgccagagag ggataaaatc tgcatctttt
    38461 gcatggattg gcgcggtctg gctcgttcga gaaaatgcgc attgatagtt ttcatatatt
    38521 gcgagagcga tttgggtagt atcctgatgt cagcctgata taatttcggg tgtagattga
    38581 aacaaggaga gaaagatgca tctaggatat ctggagatat ttgtactgat tgttttagtg
    38641 ctccttctgt ttggtcccgg tcgtatcgcc aaagtggcgg gagaactggg gcaggggatc
    38701 aaggctttta aagagggatt gagcggctca aaatctgaag aaaagagcgc aaaaagcgaa
    38761 aagctggaca accagccaga ggatgagcat caatcgaaaa aagattgatg aattttgacc
    38821 attatgcagc tatttggtgg tgtcggttta tctgaaataa ttttcatttt ggtcttgatg
    38881 atcgttgtgc ttggtccaaa aaagatggct gaaggcgcca gggatttggg caagacgatc
    38941 cgcaaagtga cccattctca attctggaag aatgtgatgc aaacgtcgcg tgagatcaaa
    39001 gatttgccaa gaaagatcat ggatgaaaca gaactggaag ataatttgcg ggagatcaac
    39061 cgcgccataa accaggactc cgccaattct cgcgagggat tgagcagccc gcctgagttc
    39121 aagcccccgc atgaacaagc agagaaatct gaccgacctg accgcacaga acgttgaata
    39181 tgccaaccga agatgaaacg ttaatgcctt tctgggctca ttttgaagaa cttcgcaagc
    39241 gacttctcat cgggctcgtt gcgcttgtga ttgcaacgtt tgtgtgcttt tcgtttgcag
    39301 aagaggctat tcgtattctc gctcagccgg ttggcggtgt gactgccctg caatcgatcg
    39361 aagtcacgga gaacatcggg gtcttcatgc gggtttccct gatgtgcggt gtgattctcg
    39421 catcgccggt tattctttat gaacttctcg catttgttat gccgggtctg aaaccagggg
    39481 agaagcgctg ggtgtttctt ggtttgagca ttggaacggt cttttttgtg gcaggcgttc
    39541 tgttcgcttt ttatatcatg ttgcccagga ccctggagtt tttggtttct tttatgaatg
    39601 tgcagactgt tcctcgcttt tccggttaca tcaagtttgt gaccaattta ttgttcttga
    39661 tcggcgtgtc ttttgagatg ccgttgatcg tttttatttt ggcaaaaatt caggtggtca
    39721 acgcaggcat gttattaaaa caatggcggt atgcgattat tctcatcgcg gtattgtctg
    39781 cggtcatcac ccctactgtt gacccggtca atatgggttt gctgatggtc cccttgattg
    39841 gactctattt tgtgagtatc ctgtttgcgt ttttggcgaa gggaaaagaa gcgaaaaagt
    39901 cggattgaca aaatccacta tgtattatgg aagggaaaca aaaggacatt ttcctgtgaa
    39961 aaaaagaaga ttagggaaca gcgctttttt tatttctgcc attggtttgg ggtgttggca
    40021 gttttcgaag aatcggggat tagccggcag tttctggggg gaccttgctg atgataccat
    40081 cacttccatc attgaagcat cactgcgggg tggggtggac tggtttgaca cggctgagtt
    40141 gtatggtcac ggcatgtcag aaagaatgct ttcgtctgcc ttgcagcggt tgaacgttga
    40201 gaaagaaaag attgtcgtcg ccacgaaatg gaaccccgtg cttcgttcag cgcgttccat
    40261 cgtgcgcacc tttcccgtgc gcgaaaaata tttggcacca tacccgataa cgcttttgca
    40321 agtgcacaac ccattcagtg tgtcaccgat tgaagatcag atgcgtgcta tggcgttttt
    40381 ggtggaaagc ggaaaggttg gtctgatagg agtgagcaat ttctctttga ctttgatgcg
    40441 caaagcccat gaaacattgc tggctgatgg tttcgttttg acatccaatc aaatgcgcta
    40501 caacctgcta gaccggtctg ttgaattcaa cggagtgctg gattatgccc gtgagcatga
    40561 catcacgctc atcgcttact caccgctggc gcagggctta cttaccgggc gttttcatga
    40621 ggaaggcgga ctgatccaga aacgccccgg ttttcggcgg tatctgccgg cattccaaca
    40681 gaggtcgctg ctcaggactc tccccttgat ccaggttttg cgcgatattg cagagagaca
    40741 ccaaaaaact ccagcacaga tagccttgcg ctggattatc cagtttcatg gagaagctat
    40801 agtagctatt cccggtgcaa gttcggtcct tcaggctcaa cagaacgcgg ctgtgatgga
    40861 ttttgagctg actgaggaag aactggcaca gatcgatgag acttcgctgg ctgttgcgcg
    40921 aaggtcatga acttctctct tccatcgcgc atatgtcgga aaagatggtg ggcagaatac
    40981 cacctttgcg gtaatactga acttccatgg gtgtgtccaa tctggcgata acctggaaga
    41041 cagagatttg ctcgtctgct ctcttcatct caaccgtgat gagctgcctg gtgtggttga
    41101 gtttctcgat cccccgcatg gagaatattt ctgttccctg gatgcccaga gtgtgcgcgt
    41161 tttcaccgtt cgtgaattga agcggcaaga ttcccatcat caccagattt gagcggtgga
    41221 tgcgttcaaa actctctgcc agcaccgttc gtacccctaa aaggaatggt cctttggctg
    41281 cccaatcgcg gcttgaaccg gttccgtatt gctttccggc gatcacgatg acaggaatat
    41341 ggcgatcttt atacagcctg gctgcctggt atatgctcgt gaacccacca gctttttctg
    41401 cgagcagttt gttttgcagt cgaggattgc tgaaagttcc tctgctcatt acctcatgat
    41461 ttccccgtct tgaaccatag gtgttgagat cagcagggtc gcagccaaat gtcagcaggt
    41521 actctccggc atcgctggtt gttgcgatat tgccggcagg ggaaatatga tcggtcgtta
    41581 tggaatcgcc caaatatgcc agcactctgg catcgcaaac gtcattctgc gtgttgaaga
    41641 aatctgtgat gaaaggaggt tccagcaaat agctggagtc aggttcccaa tcgaaaagat
    41701 accctgattt ttgctccagc tcaaaccact ttttctttcc taccagcccc tgagcgtaaa
    41761 cctcgctgta aacgctcgcg tctatcgctt tcaaatactc ccgaatttcc gcaggactgg
    41821 gcaggatctc atccagaaaa acaggcttgc cttgaacgtc tacgccaatg gg
//
LOCUS       DAOSDS010000008        41556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_529_length_41556_cov_5.592733, whole genome shotgun sequence.
ACCESSION   DAOSDS010000008 DAOSDS010000000
VERSION     DAOSDS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 41556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41556
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_529_length_41556_cov_5.592733"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..250
                     /gene="rpmG"
                     /locus_tag="PLL88_01935"
     CDS             83..250
                     /gene="rpmG"
                     /locus_tag="PLL88_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003829846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOJ00353.1"
                     /translation="MAKKRDVRPIITLECTECKERNYTTEKNRRNDPGRMEIEKYCKR
                     CKKHTLHRETK"
     gene            307..380
                     /locus_tag="PLL88_01940"
     tRNA            307..380
                     /locus_tag="PLL88_01940"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:341..343,aa:Trp,seq:cca)
     gene            445..663
                     /gene="secE"
                     /locus_tag="PLL88_01945"
     CDS             445..663
                     /gene="secE"
                     /locus_tag="PLL88_01945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOJ00354.1"
                     /translation="MSNKIEKAKTKKESGIVRWWHETVGELRKVTWPTKKEAWQTTKV
                     VLLVMVVIGTLLALLDSAFTQLISYLLS"
     gene            882..1427
                     /gene="nusG"
                     /locus_tag="PLL88_01950"
     CDS             882..1427
                     /gene="nusG"
                     /locus_tag="PLL88_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOJ00355.1"
                     /translation="MDTQEHDDGRAWYVIHCYSGYEKKVRYNLEQRIESMGMRDKIFD
                     VVIPTQEEIEVREGKRRSIERHVFPGYVLVNMILTEETWFVVRNTPGVTGFVGMGNDP
                     TPLRPEEVQHILKRMEAEAPHVKFTFREGERVRIIDGPFNDFHGHVSEIDVDRAKVVV
                     MVNFFGRETPVELDFLQVEKA"
     gene            1504..1929
                     /gene="rplK"
                     /locus_tag="PLL88_01955"
     CDS             1504..1929
                     /gene="rplK"
                     /locus_tag="PLL88_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOJ00356.1"
                     /translation="MAKKVKAIVKLNVTAGKANPAPPIGPALAGHGINIMQFCKEYNA
                     RTQNKTGEIIPAEITVFTDGSFTFILKSPPTSELLKKAAGVEKGSDMPNRNKIATISR
                     EKVREIAEVKMKDMNANDIENAMRQIEGTARNMGITIKD"
     gene            1983..2702
                     /gene="rplA"
                     /locus_tag="PLL88_01960"
     CDS             1983..2702
                     /gene="rplA"
                     /locus_tag="PLL88_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOJ00357.1"
                     /translation="MAKHGKKYLEAKTKIDKDTSYNPNEAMALVKETAYAKFDATVEV
                     HLRTALDPRQADQQIRSTVVLPNGLGKTVRVLVFAQGEGANKAKEAGADVVADDDEWV
                     KKIQDGFTDFDLAIATPETMGMAGKLGRVLGPRGLMPNPKAGTVVKVEDLPQAIQEAK
                     AGRIEFRLDKTANIHVPIGKVSFDADKLTENFSALMNAIKKAKPAAAKGIFIRKITVT
                     STMGPGVKVDINRALSMEAEE"
     misc_feature    2714..2854
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2864..3394
                     /gene="rplJ"
                     /locus_tag="PLL88_01965"
     CDS             2864..3394
                     /gene="rplJ"
                     /locus_tag="PLL88_01965"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOJ00358.1"
                     /translation="MAFTKEEKKALVEQYENWLKESHAIFVMSYSTMTMANINAVRLA
                     AREIGGEVHVVKNTLMKIALKNAGYVDSDLFSESSIVGFAFQDVPGLAKILNKSAKDE
                     NFFALKGGYLEKELIDAKQIVMLAELPSREQVRAQLLALINTPATKLVRTIQEPARGL
                     AAVLKAYSEKQAAPAA"
     gene            3444..3827
                     /gene="rplL"
                     /locus_tag="PLL88_01970"
     CDS             3444..3827
                     /gene="rplL"
                     /locus_tag="PLL88_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOJ00359.1"
                     /translation="MADIKKLAEELGKLTVLEAAELVKTLEEEWGVSAAAPVAVAAVA
                     AGNGGTTAEAAEEKTEFTVIIKDAGPKKIEVIKAIRQITNLGLVEAKALAETAGSKVL
                     EQVGKEAATDAKSKLEAAGAVVELA"
     gene            complement(4004..4750)
                     /locus_tag="PLL88_01975"
     CDS             complement(4004..4750)
                     /locus_tag="PLL88_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00360.1"
                     /translation="MNKINKHVISKLENDLGLSKRTIYRRILEKERDPKIYDKNLAAL
                     VFALENKVDIYPLLSDEEKAKIRAYYGSNPIPAIVDSSINIKKIKGGEKNTSIKIPLD
                     FITNVDLKNILDRDIKELSLAQEQGIKETAKTCIILSGSILEAIILAALKTKQKEVNS
                     YCKKTRENYNLDDLTLNELVKVASSISPPLLPPDIVPNIHQIRNWRNLIHPGRELSKA
                     KKDRISPSEGRARTAIASLHLISEEINKNI"
     gene            complement(4737..5006)
                     /locus_tag="PLL88_01980"
     CDS             complement(4737..5006)
                     /locus_tag="PLL88_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00361.1"
                     /translation="MQKELLEKMVNDISENLKIIRNSSIYNLIYSESIRKKTNIEKIA
                     ILKKIGISDRNEIAEILNTTAETVSVTISKSKKKKDNSEKINEQN"
     gene            complement(5087..5860)
                     /locus_tag="PLL88_01985"
     CDS             complement(5087..5860)
                     /locus_tag="PLL88_01985"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HOJ00362.1"
                     /translation="MSDNLSLSEIIIRGRYLFRNSPRLLNTFMNVNGKSSSNEIAKKI
                     GRNVNNINRDIKSLRNAGLIGEIKRVGKQYIYDKIPLAKEISRKDFEKGSSIPSIGST
                     IIISREKPKSTVKKIKIPDQYEILEIAKNGENQIYEFKQDGTEIKKLIREIAAMANTK
                     QGGVILYGIDDDGVIQGSSGKAQDLDQPLQNSVKDSITPSLNIEITQVDVLGSLLIVI
                     IVPPKQKGVVYLINGSAVIRKGTNVFQVKADQLKSLLDS"
     gene            complement(5857..6138)
                     /locus_tag="PLL88_01990"
     CDS             complement(5857..6138)
                     /locus_tag="PLL88_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00363.1"
                     /translation="MVDKIETTLNEIKILLAIQCKLLLNNAIDEEVKSSRVFQLTGKK
                     SRDEIVKILGVSSKTITPLWQEWEKCGLIYREGKSYIKTSEKINEIMNT"
     gene            6198..6524
                     /locus_tag="PLL88_01995"
     CDS             6198..6524
                     /locus_tag="PLL88_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00364.1"
                     /translation="MNENRECKEISRKLDQIIFLLRIQNKSSLENQINNIFSSKEEFE
                     VYDLTDGYSSIDELAEKTLVPRSTIGDWWKKWLDCGITIPGPIRNDRPQKIFSKKEIE
                     KYYKSK"
     gene            6524..7225
                     /locus_tag="PLL88_02000"
     CDS             6524..7225
                     /locus_tag="PLL88_02000"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ00365.1"
                     /translation="MYDEIATIYQQIFPVNQAFLQFIPAFITKKPTSILDLGCGPGDY
                     VDEFTRQGHLATGIDSSAQMIAWAQAHNQGSFFHLSFTEIQNLKGRFDCIYCIGNSLS
                     YLLHEASEKFFHAVFDLLEPGGSFILQLVNWDTYRLQGSMKFPIQHLADGRIFHRAYE
                     AHPGDTVLFKTEIQKDGQTLQAWADHLYPRYADSLPDEIATSGLAVSGVYGDFKRNPF
                     HAASSPALIITARRQ"
     gene            7327..8550
                     /locus_tag="PLL88_02005"
     CDS             7327..8550
                     /locus_tag="PLL88_02005"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HOJ00366.1"
                     /translation="MNPSAVIIDSVRTLIGNLGGALASIRPDDLAAHTMRALLERTKI
                     NPALVEEVYLGCANQAGEDNRDIARMASLLAGLPLETAGVTFNRLCASGLTAIINAAR
                     AIQCGEGEVFLAGGVESMSRAPYSLPKAEHAYPFGHVTLWDTTLGWRYPNPHLVDQGY
                     TINLGETAENLAEQYHISRMEQDTFALQSHQRAIAAIDSGKFAQEIAPVSIPQRKGKP
                     LVVDTDERPRRDTSLEALARLSPSFKESGSVTAGNSSGLNDGAAMLLMTSEAKAQELG
                     LQPIARVVASGAAGVDPRVMGIGPVPATQKALQRAGLRLQDIGLIELNEAFAAQALAV
                     MQALDISPDITNVNGGAIALGHPLGCSGARILTTLLHEMGRRASTHAKPFYGLATLCV
                     GVGQGETLIVEWLGS"
     gene            8682..8924
                     /locus_tag="PLL88_02010"
     CDS             8682..8924
                     /locus_tag="PLL88_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ00367.1"
                     /translation="MPLSGTIIVDENLCKACELCIVNCPVQVIALDKNRITPRGFHPA
                     ALFKEGCTGCGTCAVVCPEAAITVYREKHVEKERNA"
     gene            8931..10058
                     /gene="vorB"
                     /locus_tag="PLL88_02015"
     CDS             8931..10058
                     /gene="vorB"
                     /locus_tag="PLL88_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOJ00368.1"
                     /translation="MMSRELWKGNEAIAEAAVRAGLQAYFGYPITPQTEALEYLSWRL
                     PELGRAFLQAESELGAINMVYGAACAGARTMTTTSSPGTSLMMEGLSYIAGTEVPMVL
                     VDVVRGGPGLGNIAPAQGDYNQIVHGGGHGDYHPIVLAPASIQETINTVYEIFDIVEK
                     YRTIGIVMVDGSIGQMMEPVALPAMKEVRKVWPSWAASATEGKRERNVLTSIYLDPKQ
                     EEITNLRLMKTWQEIEKNEIRYKEYFMEDAKFAVVGFGSAGRVALSAVRAARAEGIPV
                     GLFRPISVSPFPKKQIFDLAQRLEGFFVVEMNSGMMLDDVLLASQGRIPVEFYGRMGG
                     VMPFPDEILREIRLLHADGWKNEGHPRDRWLAKLETIVKEE"
     gene            10063..10845
                     /locus_tag="PLL88_02020"
     CDS             10063..10845
                     /locus_tag="PLL88_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ00369.1"
                     /translation="MTTMNVEDMQVIYQHPTSLLDIHTHYCPGCTHGVAHRLVAEVID
                     EMNLRDQCIGVASVGCSVFAYNYFDFDFIQSPHGRAPAMATGVKRVHPGKIVLTYQGD
                     GDMISIGTGEITHAAARGENITVIFINNANYGMTGGQMAPTTLPGQKTTSSPKGRDVE
                     QAGYPIRASEMLATLDGAGYIARRSLHDPKNIRLAKKAIRTAFEAQVRGLGFSMVELL
                     STCPTNWHLAPAAAGKWLEENMLPYFPLGDYKVHTALKDLKF"
     gene            10859..11395
                     /locus_tag="PLL88_02025"
     CDS             10859..11395
                     /locus_tag="PLL88_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOJ00370.1"
                     /translation="MQNETLIAGFGGQGVLFAGKVLAHAALELGLEVTWFPSYGPEMR
                     GGTANCTVIFSDEEIGSPQVLNPTSIVVMNQPSLDKYENLVRKDGYLVVDTSMTNRAV
                     ERKDIHAICLPATESAEEIGDKRLANVVLLGALIRASNYFGQAELENALKKSLEGKKE
                     ELVASNIIALQHGFEYNS"
     gene            11570..12037
                     /gene="greA"
                     /locus_tag="PLL88_02030"
     CDS             11570..12037
                     /gene="greA"
                     /locus_tag="PLL88_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOJ00371.1"
                     /translation="MANTFLTKEGYQKLQEELDYLRGTRRAEIAERLREAAFGEDLVE
                     NAEYEAAKNEQAFIEGRIKDLEGLLATARIIESTAKGSEVVQLGSRVVIQESGNKDSE
                     EYFIVGAAEAMPAEGKISNESPLGRALLNHKEGDQVEVQAPDGSFKVKIIEVA"
     gene            12113..13636
                     /gene="lysS"
                     /locus_tag="PLL88_02035"
     CDS             12113..13636
                     /gene="lysS"
                     /locus_tag="PLL88_02035"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOJ00372.1"
                     /translation="MEWNELEKIRLGKIDQMRAQNVEPYPTRTEVDHAIKQAIDTFAS
                     FEKRNDPSISAPEVTLAGRIRSNRNMGKLAFSHIEDGSGRIQLMLRVNEIGEEGLKSF
                     NDLFDLGDFIQASGSLMRSKSGEITVLVKDFKMLAKAVTPLPAAKDEVVDGKIVKHAT
                     FSDPESRYRQRYADLAVNEEIRKIFRMRTAIVRALQKFLDQHDFIEVETPILQPIYGG
                     AAARPFSTHHNQLHQDLFLRISFELYLKRLLVGGFDRVYEIGRDFRNEGVSFKHNPEF
                     TQLEFYMAYADYEKIMSLTEQMLGYVCDEVLGTRTFSFDGHEISMNIPWKRVELRQGI
                     LDATGVDIYACPDADSLFQAMKKKGLNPKEGQPRGKLIDSLIGDFLEPSCIQPTFLFD
                     YPRDISPLAKNKPDNPQVVERFEGFVGGMELCNAFTELNDPLEQEKRFAEMGRTYDEK
                     DEERHPMDEDYLKAMAYGMPPSGGFGMGIDRLTMLLTDNRSIREVILFPHLRETEEE"
     gene            complement(13665..17375)
                     /locus_tag="PLL88_02040"
     CDS             complement(13665..17375)
                     /locus_tag="PLL88_02040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOJ00373.1"
                     /translation="MSFVHLHVHSHYSLLDGYCMFPKLIQRAKEMGMPAVALTDHGTM
                     YGVVEFYNACLKEGIHPVIGLEGYITPRRMDQRDPQKDKRSNHILLLAENMVGYQNLL
                     KIASAAQLEGFYYYPRIDHEYLADHAEGIICTSACLKGEIPSLIMNSQLELAEEQLRW
                     YLDVFGKENFYLELQDHNMSELVTVNKQLIEWAKRYQVNLVATNDVHYVDREDARYQD
                     ILLAIQTGALLTDKERMHMNGDTYYLRSPQEMAALFAEVPESISNTVAIAERCAVKLE
                     RDAYHLPKFDVPEGYDSASYLRMLCEHGLQKRYKERADAPEIRERLDYELKVIHEMGF
                     DAYFLIVWDLCKFARENDIWYEARGSSAGSIVAYTLDITLVEPLTHKLIFERFLNPSR
                     VSMPDIDLDFQDDKRAEVMQYCAQRYGSDQVAQIITFGTMKARQAIRDVGRVMGIPLG
                     EVDRVAKLIPNIPSHPLTIEEALEEVPELKEIYLNVPHMHELIDTAGKLDGVIRNAGT
                     HAAGVVISDKPLVEYLPLHRPTRESEDSPIKTVTQFEMSVLDDLGMLKVDFLGLATLT
                     IMSNTCKLVEKRHGVHLHLDNIPLDDPGTFEFLGKGLTAGVFQLEGGGMTKYLVQMKP
                     KNLDNIIAMVALYRPGPMQFIPDYIARMHGKQKVEYRHELLKPIMEETFGIAVYQEQI
                     MSAAITLAGYSPADSDTLRKAISKKKEDQIRVHEKKFIKGAVKNGIDEETARLIFDDW
                     RNFAQYGFNKSHAADYGMVAVQTAYLKTHYPLEYMTALLCGSMNDSEKVAFYIADCLA
                     MGISVLPPDVNHSFWDFSIEDQEKNGYCIRFGMGAIKNVGQAPADLIVAARKEQPFED
                     LDDFIRRVDLRKVGKRTLECLIRVGALDAFGDRRAIFNVMDRLVAISESHFRAKEEGQ
                     LSFFGAVEGLKEEIRLPELDPMDKKEKLEWERELLGLFLTDHPLSDYMKTIKRSVTHY
                     SGQLQDAQQKEEVVVAGRLTNIRSIVTKKGDPMGFANLEDVQGAIGLVIFPRTWAKSQ
                     TILVEDEVVIVRGKADVEGMDAKVLVDDIQKLEIDTNADDEEDLLQKSATDEWGLMPQ
                     DAYSEELMGSGAGFFDDLPQDMLLETDPLQNEPMAQEGEQPAPLTVEKIEKGVSEGEA
                     GYLPQVTNAKGKSLTVIINATGKPDQDIRLLQQVYGMLRASPGDDRFTFLCRENGRDV
                     VMDFPNDAIRITDDLLAKVHHLVGEENAWVRE"
     gene            complement(17472..18212)
                     /locus_tag="PLL88_02045"
     CDS             complement(17472..18212)
                     /locus_tag="PLL88_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOJ00374.1"
                     /translation="MMSTWKDYFEASKYQNMPESQQKVGQPQPPLQMAYDPAAPLILL
                     PDPKGCALACSDFRSISETRESRRSYIADRLSLVELSYLLWVTQGVKAVNESGTFTKR
                     MVPSAGSRHPFETYLLVNSVENLDQGLYRYLAIEHKLLLLDDGTDVNQRITDACLKQK
                     HVQNSAVTFCWVADIARTYWRYSERAFRYILLDAGHVCQNLYLTAESIRCGVCAIAAF
                     DDDLLNASLGLDGTHQFAAYLATLGKVA"
     gene            complement(18223..19863)
                     /gene="murJ"
                     /locus_tag="PLL88_02050"
     CDS             complement(18223..19863)
                     /gene="murJ"
                     /locus_tag="PLL88_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOJ00375.1"
                     /translation="MKTKPLSIINSMPQEHDTVHQANRQIARAAGTVMLAYILSNLAG
                     LLAKAFTARAFGTGADSEAFYAANRFSEILFNLVAGGALGSAFIPTFTGFLVKDDREG
                     AWKLASGIINWALLILTIFSILTMIFARQVVHYILAPGFSSASMEKEMLAADLLRIQV
                     PSAILFGLSGLVMGILNAHQRFFVSALAPGMYQVGWIFGALVLAPRFGVHGLSYGILI
                     GAGFHLLVQVPNLLRLPQVRYKPGLERKNPAVREVAILMAPRLLGVAIVQLNFLVNTY
                     LASLQPEGSVTAISLGFSLMLMPQAAIAQSIAIASLPTFSAQIARGKLDEMRSSFTST
                     LRMILFLAIPAMVGLIMLREDIVRLLYQRGEFTAASTNLVAWALLWYALGLIGHCIVE
                     ITSRAFYAQHDTKTPVFVGVAAMSLNLVLSILFSQLFSSIGWMPHGGLALANSTATLL
                     EALVLLSLMKKRMRGLDKKLLLSGLLKAGLAVLIMAFCVTVSMDLDVALPYWLHIALT
                     ILVAAGLYASVLYFLKVEEIKTLLHLVLGKLSRQKTVH"
     gene            19937..20557
                     /locus_tag="PLL88_02055"
     CDS             19937..20557
                     /locus_tag="PLL88_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00376.1"
                     /translation="MEQKKGNTTTSNAAPQVNAGSQKSQQPPTAKVEGKAGVKVHKNS
                     WTQKALRQFLLAIVFLAIGAGLIFFLIHQPAAKEYVAQKEEATRLQDIESQYNQLLTD
                     YADLDTRHKVSVTLVNIYKIQNNVNIARIALMNDNETRLSIALGYVEKDIATLDIGEF
                     PEKVDLSARVTAIKGYLPDEKQNALNELNALFDDLLLLANNMEITE"
     gene            complement(20606..21772)
                     /locus_tag="PLL88_02060"
     CDS             complement(20606..21772)
                     /locus_tag="PLL88_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ00377.1"
                     /translation="MIKSRKILFSSLVFAGLILSSCGVVQEMQELPVEITTATEEGRD
                     AVPDQVLEYTQGEDMLVNIYKSVNPGVVSIVVYTTNGGAGQGSGFVYDLEGHIITNYH
                     VIENAAEIEITFSDGARTRGRVIGTDTDSDIAVVKVDMLAEELHPLILGNSSELEVGQ
                     FVIAIGNPYGFSGTMTTGIISAMGRVLDSIRSSGSGSPFGAGDTIQTDAAINPGNSGG
                     PLLNLEGEVIGINRAIYDQTSSIYESASYSGIGFAIPIDMVKKVVPSLISEGSYDYPY
                     LGISSVDGLTMDEIEAMGFDPNVPGVYVLDVTPGGPAARAGIRAGTRATKYQGLYAGG
                     DLIVAVDGVTTKNYNQLLTYLFMNKAPGDTMVVTVLRNGAERDISVTLGSRSGQ"
     gene            complement(21769..22728)
                     /locus_tag="PLL88_02065"
     CDS             complement(21769..22728)
                     /locus_tag="PLL88_02065"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOJ00378.1"
                     /translation="MEYKDYYAILGVGKDATGDEIKKAYRKLAMKYHPDKNPGNKQAE
                     ERFKEINEANEVLSDPKKRSRYDQLSNSYQSWQRAGNNPNTFRWEDLFNNPQYTTRTT
                     RVNNVDDFEDLFGGGLGGFSDFFNAFFGTPRSRSQRRNAQAPSAGRSARPQAYQQPVT
                     ISFWEAYHGTSRLLQFSDKKIEVKIPAGVRTGSKVRIVGAGPRQADGTHADIYLVITL
                     APDKQFAVDGDDISTTITIDVGTALLGGEEKVHTPSGDVLLKIPPGTQPMQIFRIKGH
                     GLPSLKQKDKFGDLMVKVKVEIPRNLTEKQRSTLRDLWKKEDK"
     gene            complement(22992..23411)
                     /locus_tag="PLL88_02070"
     CDS             complement(22992..23411)
                     /locus_tag="PLL88_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HOJ00379.1"
                     /translation="MQAYFSCSITGGRTEQHIYQQIVDHLIERKWRVPTAHLAESGVP
                     DEEGMIDPRVVYERDVRWVLESDLLIAEVSTPSHGVGYEIALAEMNKKPVFCCYRKGK
                     KVSKMILGNENPIFCVYAYEGIDDLLKVLDEFLDDYV"
     gene            23484..25346
                     /gene="uvrC"
                     /locus_tag="PLL88_02075"
     CDS             23484..25346
                     /gene="uvrC"
                     /locus_tag="PLL88_02075"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOJ00380.1"
                     /translation="MTSAKEHIENILDTLPTKPGCYLMKDSSGKIIYVGKAINLHNRV
                     RSYFQSAADHSLKTQHLVRDIVDIDWIVVGSELEALILEMNLIKKHRPPYNVRLKDDK
                     RYPYIKAHWNEPFPKVTVTRNLENDGGKYYGPYTSVWAVHQTLDLLRKIFQYRTCDRI
                     ITGKDPRACLYYDIKLCSAPCIGKINESDYNQMITDLGKFLEGQTDPILDRLRTRMEE
                     MAETLQFEKAAALRDQVQAIERVVEKQKVISTQNMDSDVIALARSNGEACVQIFFVRA
                     GKLIGRDYFILEGADDEKDSEVIAQFLTQFYSRSAHIPSEVLLPEDIEEAMIIQNWLN
                     QKKKDEKIQIRVPRKGVPKQLVEMATENAMETLRSLKLQWEMDTNKQSVALAELQAAF
                     GMNNPPNRMECYDISNTQGTASVGSMVVFEQGVPNKKHYRRFNIKTVNQPDDFASMEE
                     VLTRRFRRWQAYHESDEVGKKAEPSFSILPDLLIVDGGKGQLGRALKVLEKFGLQNSI
                     KAVGLAKSEELVILPDQAEPLALPRNSQGLFLMQRIRDEAHRFAITAHRNQRSKNSLR
                     SILDEITGIGATRKKALLAEFGSVENLRQASVEDIAKLPGISRSLAEEILRSIQ"
     gene            complement(25373..25945)
                     /locus_tag="PLL88_02080"
     CDS             complement(25373..25945)
                     /locus_tag="PLL88_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00381.1"
                     /translation="MKKLLFILLVLFLYGCTANNTAEVNALQTQVAVLSGADQAVAEE
                     APADFVAAQSDENAFVLVLTEKLTAKGWTVSNVEGSTFGITTPSGGIYLIEYDYQNEK
                     ISRMIIYSLWIGAGTSNLEPEVISAVNQANDEQFLAKVSVDAEGDFWLETVLPFDSQL
                     DVNFFCDYLEWFEDSEPVLLLNYFQDYLQQ"
     gene            26120..26467
                     /gene="rplS"
                     /locus_tag="PLL88_02085"
     CDS             26120..26467
                     /gene="rplS"
                     /locus_tag="PLL88_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOJ00382.1"
                     /translation="MSELLKAVETEKNPNIPELAPGDVVSVHVKIKEGDRERIQEFKG
                     TVLFVSMSGQSSTFTVRRVASNGIGVERTFLFRSPKIDKVVVERHNKVRKARLFYLRE
                     RTGKRARLKQKFS"
     gene            26538..27377
                     /gene="murI"
                     /locus_tag="PLL88_02090"
     CDS             26538..27377
                     /gene="murI"
                     /locus_tag="PLL88_02090"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOJ00383.1"
                     /translation="MMNRTSSKSLKVGVFDSGVGGLSVLRSIHDRLPHPEIVFFADQA
                     HVPYGRRQLDEIRSFSQQITLFLLERDIDLLVVACNTASAAALKFLRQQFPRLPIVGM
                     EPAVKPASEQTRTRAVGVLATPATFQGELYQSLVHKYGEGLQIYQDTCPGLVEEIEAG
                     DLTGEKTRSILENALLPMIELNVDTIVLGCTHYPFVIDTMRRIAGKHIQVIDPAPAVA
                     RRVQALLNQQFPERMPIASSGSTQFFTSGDPLVLQKQIKLLIGLETTVRSAHWEDGKV
                     YAD"
     gene            27422..28453
                     /locus_tag="PLL88_02095"
     CDS             27422..28453
                     /locus_tag="PLL88_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOJ00384.1"
                     /translation="MRSIAIIMAGFGNVGKAFARLVYRKYAEIENAYGFDLRITGVST
                     GKHGCLGMETGFDLEQLASGVLSSSSLPGSTPFRDTLEMIEKSEADVFIENTPVNYES
                     GQPAVDFLTHALEKAMHTVTANKGPVVHAYEKLSDLARQKGKRFLFESTVMDGAPVFS
                     VVREALPAMQIAGFEGILNSCTNLILCRMEEGETFEQAVRYAQSIGIAETDPSGDIDG
                     WDAAVKVAALSTVLLNHPVTPQQVQRNGIREISAADMRSARAAEKRWKLICSAQKTTG
                     GWDLSVAPHMVGAESPFFSVNGTSSYILFKSDVLPGLGLLESDPSPDTTAYGMLADIL
                     NIFKQDRVV"
     gene            28450..28950
                     /gene="folK"
                     /locus_tag="PLL88_02100"
     CDS             28450..28950
                     /gene="folK"
                     /locus_tag="PLL88_02100"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOJ00385.1"
                     /translation="MKHHVFLGIGTNLGDRPANLQKAIATLQRVIQVEQTSKVYETPP
                     WGFADQPAFLNQVLSATTRLGPFELLAEIKTIETEIGRTPTFRYGPRLIDIDILFFDD
                     LVLDEERLTIPHPHLTERAFVMVPLNEIAPQHVHPRLQRTIQDLMPEVDISNVKLYEN
                     SSPLEK"
     gene            28953..29816
                     /gene="folP"
                     /locus_tag="PLL88_02105"
     CDS             28953..29816
                     /gene="folP"
                     /locus_tag="PLL88_02105"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOJ00386.1"
                     /translation="MEAKSMLPFGTRTFVMGIINLTPDSFSGDGLMKSSAMAEAAVQQ
                     AIRFTEQGADILDLGAESTRPGAETLDASSELERLLPALERIVALKLNTIISIDTYKA
                     SVAEACLQAGAGMINDIWGLKHDPQLAGVAARHAVPVVLMHNRSHAKFLQKSETLGGR
                     YVGTTYQDLMNDICSDLQESITIAKTAAIQDQNIILDPGIGFGKTVEQNLSIIHHLGE
                     LRRLGYPILLGASRKSFIGYTLDLPVEDRLEGTAAVVALGINNGADIIRVHDVQAMVR
                     VARMCDAILRN"
     gene            complement(29813..30472)
                     /locus_tag="PLL88_02110"
     CDS             complement(29813..30472)
                     /locus_tag="PLL88_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOJ00387.1"
                     /translation="MIDVSNITVTRNQLLATKQQLGLTRQGYELLDKKRIALIQKIVE
                     LERKVLEEAQRLQLSTNQAKMALVRAEAFVGEGRVKAASLGAQSEYSIELDEEIVMGV
                     RVPKIRQKRDEEKESTQPYVFNSISSMIEEAGVAFRNNVADILKLADEEVQLTALLKE
                     IAHTTRRLKALELIVIPRLQGEYAYIRDELDERERADHFRLKQAKNIIEQRNVKDQAR
                     I"
     gene            complement(30469..31887)
                     /locus_tag="PLL88_02115"
     CDS             complement(30469..31887)
                     /locus_tag="PLL88_02115"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOJ00388.1"
                     /translation="MPEKLMNTKLATKEYRTLSQIKGPLIFVERVAGVAYDEMVQIID
                     PDGNVRMGRVLEIAGDMAVIQMFLGTEGLDISRTGVRFSGDVVRLKVSLSMLGRILNG
                     MGEPIDGGPAIVPEKLEDINGSPINPAVRNEPQEFIQTGISTIDGLNSLARGQKLPIF
                     SGSGLPGNELAAQIASQARVLTEKKESDEPFAVVFAAIGITHRESSFFIEHFRSSGAL
                     NRTIVFLNHADDPTIERIMTPRVALTAAEYLAYTHNLHVLVILTDMTNYCEALRELSV
                     AREELPGRRGYPGYMYSDLASLYERAGRIKGKQGSVTQLILLSMPDDDITHPIPDLTG
                     YITEGQIVLGRTLNQKGIFPPVDVLPSLSRLMKEAIGEGHTREDHQSVADQIYSFYAE
                     GRELEKLVAIIGEASLTEQDQRILNFSKNFEQKFVNQGNENRSIEQTLDLAWELLSEF
                     PKESLKRISEALIEKYYRGERA"
     gene            complement(31880..33688)
                     /locus_tag="PLL88_02120"
     CDS             complement(31880..33688)
                     /locus_tag="PLL88_02120"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOJ00389.1"
                     /translation="METMIKETGRINRISGPVVGVSGLEGIRLHDMVLVGEAKLVGEV
                     IRMSGQQATIQVYEETSGLRMGEPIAGTGHPLIAHLGPGLMGQIFDGLQRPLNKLADI
                     EGDFLKKGVTEDSLPQDKKWKFTALVKEGDLVKSGNLLGEVPETQSILHRVMVPFSVN
                     GRVVEVHDGELTLTQPVVVIEDGKGRITDVKMEHVWPVRSARPYKRRLDLAEPLVTGK
                     RVIDAFFPIPKGGSAIIPGGFGTGKTVIQQSLARWADVDVVVYVGCGERGNEMAEVLM
                     QLPELEDRVKGIPLINRTVLIANTSNMPVAAREASIYLGITVAEYYRDMGYDVLMLAD
                     STSRWGEALREVSGRLEEIPGEEGYPAYLSSRLAEFYERAGHVDCLGNGENKPYAGHR
                     EGSVSLVGAVSPPGGDFSDPITQSSMRIAGVFWALDYELSRKRHFPAINWIKSFSLVD
                     LSRWFSDTVEEDFPQLVQEAKSLLGRENELLQVVQLIGQDALSDVEREILAISKLLRE
                     DYLQQSSIQDVDTYCSLEKSYWMLKVILTYHQRAQAALKTGIDFSEITNLTVLQRIAR
                     MKEIPLERSTAELKRLTGKIHDTFDALVSSWETENA"
     gene            complement(33672..34022)
                     /locus_tag="PLL88_02125"
     CDS             complement(33672..34022)
                     /locus_tag="PLL88_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOJ00390.1"
                     /translation="MNKLVVITIPSMANGYRLAGLEAYGVTDSTALAELLEEFIEKSE
                     PLLLAIDDDLFSHLDQKTVAALRNCKNLALVTIPGRTGKMNKKTSQEYLSKMVRHATG
                     TQFHLKGDENGNDD"
     gene            complement(34019..35080)
                     /locus_tag="PLL88_02130"
     CDS             complement(34019..35080)
                     /locus_tag="PLL88_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOJ00391.1"
                     /translation="MSTFDYGNTRLRARFPQLIMKEMLEEMTRADNLDGYLSLLIKSS
                     YKPSIEKALTVSSGIQAVHNIIEFEMEKLFLDYRNFYHESSWNLIGLIFSHNDLQNLH
                     TIVRGVLGSVPYREIQELLNRSGTVPMHILQELARSRNLSDLISKMIAFHLPYTDALL
                     KNQTSLFTMQGAQIELLLEKEFYERYLSRKKHILNRNKLLKEYFDMNADRENIICALR
                     IVQNPDMLIRNGLAIKECFLQAGSFERAFLISVANESDISRAVSHFTRSRYFASLQDG
                     LLAYGKSGLLAEFEERLRKQLLAWASRYALKDPIGIGVPIGYMVRKQNEMQNLWWIAK
                     GIQLGFDSVDIIEHLEILQ"
     gene            complement(35080..35694)
                     /locus_tag="PLL88_02135"
     CDS             complement(35080..35694)
                     /locus_tag="PLL88_02135"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOJ00392.1"
                     /translation="MGIEAILAGINAITENKIAELQQQTVQQVNQILFRSKEEVEEIR
                     KNLEKEGRVRLNREAAIIQQQAEMQYLQNISDARQKLIQESLGQIEIALRSIRTQGEY
                     PALLRSLIKEAVGDLQPSLGQEKEIIVSVDPKDKELLNKFPKDGSVKIHFQYDLDCWG
                     GCNASSLDGKVCVYNTLNDRFQKAVPILQQWLSLFFSEQVTGMD"
     gene            complement(35710..35922)
                     /locus_tag="PLL88_02140"
     CDS             complement(35710..35922)
                     /locus_tag="PLL88_02140"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00393.1"
                     /translation="MEKGIIALAAALAIGLTALATAMAQRAIGSAGVGAVAEKPELLG
                     SIIILVAIPETMVILGFAVAAIILMG"
     gene            complement(35950..37848)
                     /locus_tag="PLL88_02145"
     CDS             complement(35950..37848)
                     /locus_tag="PLL88_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOJ00394.1"
                     /translation="MIRKMARIEIVGLKKDLQNTIHELHNLGAMQIDDIRSLSNLLLQ
                     PLNLTYEMVQKREKVELINARINGFADLFKKYLVGSVENLEDNSTTEEIGEKVDQVTT
                     IVQQFTQNRTALEEDLITLSKYQEILKLMAPLLPESARKPGNATVGALLHATQTHSMN
                     MLVNQLRTLTQGKFEMAIVKVNGSTNALIGVLPREFVVSVEELLTKEKITQLVLPEEY
                     SLLSASEALEMTTKRIAEDHRELDALEGHFRELANAWGPFLRNWQVTCLNLLDEFEIY
                     SRLGETEFTFTAYGWVPADELQDLKSRLQEKIGEDLVINQLEIPKEMHKKIPVAMENS
                     QSIKPFENLTKMRAIPAYSDIDPSTLMAIFMPLFFGMMVSDVAYGAILFFLTLFLAKK
                     IKTGIFADLIKTIKIGSLWAIGFGFLFGEFMGTMGSSIGLKPVLLDRTASGSAITLLY
                     MTIGVGVVHILLGLILGIANALKHKNKSHLLEKGGMFIGIMGLLVVVGATLKVIPAAG
                     RWGGYALLAAGVIFLGISYGKTGMILGPIEFIGLLGNILSYLRIAALGLASVFLAKVA
                     NDMVGMMGNVIAGVLVSILIHSLNIIMAAFSPTIQSLRLQYVEFFRRFYEGGEQGFTP
                     FKKRILDH"
     gene            complement(37823..38152)
                     /locus_tag="PLL88_02150"
     CDS             complement(37823..38152)
                     /locus_tag="PLL88_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00395.1"
                     /translation="MQAEIRSAERISEVKKDADRQIAKVQEDAENSKKEAYANGRRAR
                     ARLIENGILRANAAAEEKLHLSESKTSKIIENGKKFIPQAVALGLDFILGSGSKEISY
                     DSQNGKD"
     gene            38391..39788
                     /gene="lpdA"
                     /locus_tag="PLL88_02155"
     CDS             38391..39788
                     /gene="lpdA"
                     /locus_tag="PLL88_02155"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOJ00396.1"
                     /translation="MKEYDVIIIGAGPGGYVAAIRTSQLGLRTAMIEKRWLGGVCLNV
                     GCIPSKALLKNAEIARTLREEAKEYGIQGENITVDYSAAVKRSRQVSDRLTRGVSFLM
                     KKNKIDVYEGSAFLKAKDLVAVTLKDGKSEELKAGNIILATGAHPFMAPEWNHDGKKV
                     LTYEDAILQEKLPTSVIIIGAGAIGAEFATIWSSFGTKVSLVEMLPNILPLADEELSK
                     ELQKAFQKRGIEIHTGHRVETIEPTSNGVKVTLSTGNEKKLLEAEQALVAIGFKANTD
                     ELGLEAVGVTLDKRGFINVDANMATSIPGIWAIGDVTGKLLLAHAASAMGIHCAETIK
                     GIKRKPIDFKMIPSATYSHPQVASFGLTEKEAIEAGKQIKKGKFPFQANGKALGLGES
                     SGFVKVIVDANYGEILGAHLIGPEVSELLPELSLAQQSELTVEELAHNIHAHPTLSEA
                     IMEAAEDALGRAIHI"
     gene            39900..>41556
                     /locus_tag="PLL88_02160"
     CDS             39900..>41556
                     /locus_tag="PLL88_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00397.1"
                     /translation="MLKKKFRASDKTSVSKDFKKFTPLLNEINDLEKTFEPLSDQELA
                     QKTILFKQQLENGKTLSDILPQAFAAVREASKRTLGLRHYDVQLIGGIIMHEGGIAEM
                     RTGEGKTLVATLPLYLNALSGKGAHLVTVNDYLARRDARWMAPIYSALGLSVGVLQMA
                     NRTENGHMAFLVDLEKHASKEEDDQLQIVPRREAYQADITYGTNNEFGFDYLRDNLVS
                     RIEDRVQRAHHYAIVDEIDNILIDEARTPLIISGSASEDIAWYEKMALIVRQLDEEDY
                     EFNEKDRSVSLTEVGEVHAEELLNTTLRDPDRPEDITPEQARLLGYLEQALKARLLFT
                     KNKDYIIQNGEVIIVDEFTGRLMPGRRWSEGLHQAIEAKEGVRVHAENITQATITLQN
                     YFRMYEKLAGMTGTAYTEAEEFYEIYKLDVVPIPTNLDYSAGSPTSDLNSYQKKDDEG
                     YEYTYYAAKSDPEQKELFWKRKDYPDIVYQSREAKLRAICIEITRFNAIGRPQLVGTT
                     SIIHSEELSARLKRESLRLLMQILVLKSLWREKFGINDAERAIP"
BASE COUNT        11096 a        10439 c         9710 g        10311 t
ORIGIN      
        1 tccgcgaggg cggtctcacc gtcggcgctg gcgtcatcac tgaaatcttg gagtaaacca
       61 gaaatatctg ggataaaaga gaatggctaa aaagagagat gtccgtccaa taatcacatt
      121 ggaatgtacg gaatgtaaag aaagaaacta tacaaccgaa aagaatcggc gaaacgatcc
      181 gggtcgcatg gagattgaaa agtattgtaa acgctgtaaa aagcacacct tacatcgcga
      241 aacgaagtaa aaacgcggtt acaatgcggg tgcgatgtga gattttcatg cacccgcact
      301 ttttacaggc cagtagctca attaggcaga gcgcctgtct ccaaaacagg aggttgtggg
      361 ttcgattcct gcctggcctg ccttatacaa agcgacgcca gatttaccgg cgtcgtttta
      421 gacatagaaa caaggagttg ctgtatgtct aataaaattg aaaaagcgaa aacgaaaaaa
      481 gagagcggta ttgtacgttg gtggcatgag acagtcggcg agctccggaa agtaacctgg
      541 cccaccaaaa aagaagcctg gcagacaacc aaggtcgtcc ttttggtcat ggttgtcatc
      601 ggaacgctgc ttgctttact ggattccgca tttacccaac tgatttccta tctgctttcg
      661 tagtgtggat gttgtcgttg cggtaagtgt tttaaaagag gtaaagaatg gcatacgaag
      721 aagagttcaa tgaacaagag aattttgaat ccgaaagtcc gctttcggaa gaggattctt
      781 cccactcgga tcgatccgcg gcatctgaag atgaaaagat ggattatctc gaaccttctt
      841 cttcagaagc agatgcagcc gagccgtttg atgttggaaa gatcgatacg caggaacatg
      901 acgatggtcg tgcctggtat gtgatccatt gttattcggg ttatgagaag aaagttcgct
      961 acaatctcga acaacgcatc gaatctatgg gcatgcgcga taagattttt gatgtggtta
     1021 tccccaccca agaggagatc gaagttcgtg aagggaaacg ccgttcgatc gaacggcatg
     1081 ttttccccgg ttatgtattg gtcaatatga tcctgaccga agagacctgg tttgtcgtgc
     1141 gcaacacgcc tggcgtgacc ggctttgtgg gcatgggcaa cgaccccacc cccttgcgac
     1201 ctgaggaggt ccaacacatt ctcaagcgca tggaagccga agcgccgcat gtaaagttca
     1261 ccttccgcga aggcgaacgc gtgcgcatca tcgatggacc gttcaatgat tttcatgggc
     1321 atgtttctga gatcgatgtg gatcgcgcaa aagtcgtagt aatggtaaac ttcttcggtc
     1381 gagaaacacc tgtagaactg gatttcttgc aggtagaaaa agcgtagact tcctccttgg
     1441 ttggaggttg ttgtgggagg gtgacccaaa ttcacccgct aaaaccactt aaggagaaaa
     1501 gctgtggcaa agaaagtaaa agcaatcgta aaacttaacg taacagccgg aaaagcaaac
     1561 ccggcacccc ccattggtcc agccctggct ggtcatggca tcaatatcat gcagttctgc
     1621 aaggaataca atgccagaac gcagaacaag acaggtgaga tcatccccgc cgagatcacc
     1681 gttttcaccg acggttcttt caccttcatc ctgaaatcac cgccgacctc tgaactgctc
     1741 aaaaaagcag ccggcgttga aaaagggtct gacatgccca atcgcaataa gattgcaacg
     1801 atctcccgcg agaaggttcg tgagatcgcc gaggtcaaga tgaaggacat gaacgccaat
     1861 gatattgaaa acgcgatgcg ccagattgaa ggcactgctc gcaatatggg aatcacaata
     1921 aaagattaat cgtttgtggg agggtgagcg agcttacccg acaacaccac gaaggagcaa
     1981 taatggcaaa acacggaaaa aaatacctgg aagcaaaaac aaagatcgac aaggatactt
     2041 catataaccc caatgaagcg atggctctgg taaaagagac agcctatgcc aaatttgatg
     2101 cgactgttga agtccatctg cgcaccgcat tggatcctcg ccaggctgac cagcagatcc
     2161 gcagcacggt agtgctgccc aacggactgg gaaaaacagt gcgtgtgttg gttttcgcac
     2221 agggtgaagg tgccaacaag gcgaaagaag ccggtgcgga tgtggtggca gatgacgatg
     2281 agtgggtgaa gaaaattcaa gacggtttca ccgattttga cctcgccatc gccacgcctg
     2341 aaaccatggg aatggctggc aaacttgggc gtgtgctcgg tccgcgcggc ttgatgccaa
     2401 acccaaaagc cggcaccgtg gtcaaagtgg aggacctgcc tcaggctatt caagaagcaa
     2461 aagctggtcg tatcgagttt cgccttgata aaactgcgaa catccatgtg cccatcggaa
     2521 aagtgtcatt cgatgccgac aaattgaccg aaaacttttc cgccttgatg aacgccatca
     2581 agaaagccaa accggcagca gccaagggca ttttcattcg caagatcacc gtcacctcaa
     2641 ccatgggtcc cggtgtaaaa gtggatatca atcgcgcact ttccatggaa gcggaagaat
     2701 gatataatct gcgcttgata gatacctatc acccaagaca gccggtgcca gaaatggctt
     2761 aatatcctgc tcaggtgaag tgaaaagttc atctttttac gtacagagat aatctctgcc
     2821 tgagcaacat cggcagagat ttttttgaaa ggaggtactt tcattggctt ttacaaagga
     2881 agagaaaaaa gcactggttg agcagtacga aaactggtta aaagaaagcc acgctatttt
     2941 cgtgatgagc tactctacga tgaccatggc aaatatcaac gccgtgcgct tggctgcgcg
     3001 cgaaattggg ggagaagttc acgtggtaaa aaacacgttg atgaagatcg cattgaaaaa
     3061 tgccggctac gtggattctg atctgttcag cgaatcatcc attgtcggct ttgcgtttca
     3121 agatgtgcct ggtttagcca agatcttgaa taaatccgcc aaggatgaga acttctttgc
     3181 tctgaaaggc ggttatctcg aaaaagagct cattgacgca aaacagatcg tcatgctcgc
     3241 agaactgccc tcacgcgaac aggtacgtgc tcagctgttg gcactcatca acaccccggc
     3301 taccaagctg gttcgcacca ttcaggaacc agcccgtgga ttggctgcag tgctcaaggc
     3361 atattcagaa aaacaggctg ctccggcagc ttaatggatc gatcaaaaat taggaacaat
     3421 aaggaattta aggagtatta cagatggcag atatcaagaa acttgcagaa gaacttggca
     3481 aattaaccgt tctcgaagca gcagaattag tgaaaaccct ggaagaagaa tggggcgttt
     3541 ccgccgccgc tccggttgcc gtagcagccg ttgccgccgg taatggtggc acaaccgccg
     3601 aagccgcaga agaaaagacg gaattcaccg tcatcatcaa agacgccggt cccaaaaaga
     3661 tcgaggtcat caaagccatc cgccagatca ccaatttggg tctggtagaa gccaaagctc
     3721 tggcagaaac tgccggttcc aaagttttgg aacaagtcgg caaagaagcc gccacggatg
     3781 caaaatccaa actggaagca gccggcgcag tggtcgaatt agcctaaacg ctaaattact
     3841 aaggggctgt ctaaaaagca gacagctcct tttatttttt ccactttagc ccgaaaatag
     3901 gggaaatttg ggacagaagg taaaattctc ttcttttttg agtattgttt cccactttgc
     3961 gctgtctaaa aagtaaaaat cagacttttg ggacagcctc tttttatata tttttattta
     4021 tttcttcaga aattaaatgc aacgaagcaa ttgcagttct cgctcttccc tcggatggac
     4081 tgattcggtc tttctttgct tttgatagtt ctcttccagg atgaatcaaa tttctccaat
     4141 tgcgtatttg atgaatattc ggaacaatat caggtggtaa taatggcggt gaaatactag
     4201 atgccacttt tacaagttca tttaatgtaa gatcatcaag gttatagttt tcccttgttt
     4261 ttttacaata actgtttact tctttttgct tggtttttaa tgctgcaaga attatagctt
     4321 ctaaaatcga acccgacaaa attatacagg tttttgcagt ttctttaatc ccttgttctt
     4381 gtgccaaact taattcctta atgtctctat caagaatatt ttttagatct acattagtaa
     4441 taaaatcaag agggattttt attgaagtat tcttttcacc acctttaatc ttttttatat
     4501 ttatagaaga atcaacaata gctggtattg gattggaacc ataatatgct cgaattttag
     4561 ctttctcctc atcagaaagt agaggataaa tatcaacttt gttttccaaa gcaaaaacca
     4621 aagcagccaa atttttatca taaattttgg ggtctctctc tttttctaaa attcttctat
     4681 aaattgttct tttagataaa cctagatcat tttctagttt actaattaca tgcttattaa
     4741 ttttgttcat ttattttttc actattatct tttttctttt ttgattttga aatagtgacg
     4801 ctaactgtct cagcagtagt atttagaatt tctgcaatct catttctatc tgaaataccg
     4861 atttttttta gaatggcaat cttttcaata tttgtttttt ttctaataga ttcagaataa
     4921 attagattat aaatagatga gttcctaata attttcaaat tctctgaaat atcatttacc
     4981 attttctcta ataattcttt ttgcatgttg tctcctttct ccaaatctat agaatacaca
     5041 tacttttttc cattcaaatc aatattcaat ccttttttat ttagtactag ctatctaata
     5101 aagacttcag ttggtctgct tttacttgaa aaacattagt tccctttcgg attactgcac
     5161 taccattgat taaatagaca acaccttttt gttttggagg aacaataatt acaattaata
     5221 gtgaacctaa aacatccact tgtgtaattt caatatttaa tgagggggta atactgtcct
     5281 ttacggagtt ttgtaggggt tggtctaaat cttgtgcttt acccgaactt ccctgaataa
     5341 caccatcatc atcaattcca tataaaataa cacctccttg ttttgtattt gccattgcag
     5401 caatttctcg aatcaacttt ttaatttcag tgccgtcttg tttgaactcg tatatttgat
     5461 tttccccgtt ttttgcaatt tcaagaattt catattgatc gggtatttta atttttttca
     5521 ccgtactttt aggtttctca cgagaaataa taatggtaga tcctatgcta ggaattgacg
     5581 atcctttttc aaaatccttc cgtgatattt ctttagctaa tggaattttg tcgtagatgt
     5641 attgttttcc gactcttttt atttcaccga ttaaaccagc gttacgcaaa cttttaatat
     5701 ccctattaat gttatttaca tttcgcccga ttttttttgc aatttcattt gatgatgatt
     5761 tcccattcac attcatgaaa gtattaagca atcggggaga atttctaaat agatacctcc
     5821 ctctaattat tatttctgat aaagataaat tgtcgctcat gtattcataa tttcattaat
     5881 tttttcagaa gtttttatat atgattttcc ttctctataa ataagaccac atttttccca
     5941 ctcctgccat aatggagtaa ttgtttttga agaaacacct aaaattttaa caatctcatc
     6001 cctacttttc tttccagtaa gttggaaaac tcgtgaactc ttgacttctt cgtctattgc
     6061 attattaagt aaaagtttgc attgaattgc taacaaaatt tttatctcat ttagtgtagt
     6121 ttctatttta tcaaccatta atttcctcct ttgcatcgca tataataaat attatatgaa
     6181 atggaggatt atttcaaatg aatgaaaata gagaatgcaa agagattagc cgaaaattag
     6241 accaaataat ttttttgtta cgaatacaaa ataaatcttc tctcgaaaat caaataaata
     6301 atattttctc ttctaaagag gaatttgaag tttatgattt aactgatggt tattcatcaa
     6361 ttgatgaact cgctgaaaaa acacttgttc caagatctac aattggtgat tggtggaaaa
     6421 aatggcttga ttgtggtatt accattcctg gtccaataag gaacgatcga ccccaaaaaa
     6481 ttttttccaa aaaagaaata gaaaaatatt acaaatcaaa ataatgtacg acgaaatcgc
     6541 aaccatctat cagcagattt ttcctgtgaa ccaggcattt ctacagttca tcccggcatt
     6601 catcacgaaa aagccaacca gcatcctcga cctgggctgt ggaccgggag attacgtaga
     6661 tgaattcacc aggcaggggc acctggcaac cggcatcgat agcagcgcac agatgatcgc
     6721 ctgggcacaa gcgcacaatc agggcagttt ctttcacctc tccttcaccg aaatccagaa
     6781 tctcaaaggt cgctttgatt gcatctactg tatcggcaac agtctctcct acctgctcca
     6841 cgaagccagc gagaagtttt tccatgctgt tttcgacctt ctcgagccag gcggttcgtt
     6901 cattctccag ctcgtcaatt gggacacata ccgcttgcaa ggaagcatga aatttcccat
     6961 ccagcatctc gcagatggca gaattttcca tcgggcatat gaagcgcacc ctggcgatac
     7021 ggttttgttc aagacggaga tccaaaagga tgggcaaacg ttacaagcct gggctgatca
     7081 tttgtacccg cgctatgctg attctcttcc agatgaaatc gcgacaagcg ggttggcggt
     7141 tagcggggtg tatggggact tcaaacgaaa cccgttccat gccgccagca gccccgcgct
     7201 gatcatcact gcccgcaggc agtgaattct ctatggacaa tgttgtccgt tccggtgata
     7261 aaagcccttg catcaagggc ttttttcttt atacaataag aacatattga tcataaggaa
     7321 cttttcatga atccatctgc cgtcatcatc gattccgtcc gcacccttat tggtaatttg
     7381 ggaggagcgt tagccagcat ccgcccggac gacctggctg cccataccat gcgtgctttg
     7441 ctggagcgca caaagatcaa tcctgccctg gtggaagagg tttatttggg ctgcgccaat
     7501 caagccgggg aagacaaccg cgacatcgcg cgcatggctt ctctgctggc ggggctaccc
     7561 ctggaaacag ccggggtcac cttcaaccgg ctatgcgcct cggggctgac agcaatcatc
     7621 aacgcagcac gcgccatcca atgcggcgaa ggagaggtat tccttgccgg cggggtcgaa
     7681 agcatgtcgc gtgcaccgta ttcactgccc aaagccgaac atgcgtatcc tttcgggcat
     7741 gttaccctgt gggatacgac cctcggctgg cgttatccaa atccccatct ggttgaccaa
     7801 ggttacacca tcaacctggg tgaaaccgcc gagaacctgg ctgagcaata ccacatcagc
     7861 cgcatggaac aagacacgtt cgccttacaa agccaccagc gagccatcgc tgccatcgat
     7921 agcggtaaat tcgctcagga gatagcgcca gtcagcatcc cgcagcgcaa ggggaagcca
     7981 ttggtcgtgg acaccgacga acgtccacgg cgcgacacca gtttggaagc gcttgctcgc
     8041 ttgagccctt ctttcaagga aagtggaagt gtgaccgccg gcaactcttc tggcttgaac
     8101 gatggagcag ccatgctgtt gatgacgagc gaagccaaag cacaggaact gggtttacaa
     8161 ccgatcgcac gggtggtcgc cagcggcgct gccggggtgg acccgcgcgt gatgggcatt
     8221 ggacctgtgc ccgccacgca aaaagcgctg cagcgcgccg ggctgcgcct gcaggatatt
     8281 gggttgatag aactgaacga agcatttgcc gcacaagccc tggcggtgat gcaggcactg
     8341 gatatctccc ccgacattac aaatgtcaac gggggtgcca tcgcgctggg tcacccgttg
     8401 ggctgctcag gcgcgcgcat tctgaccacg ctgctgcacg agatgggcag gcgtgcatcc
     8461 acccatgcca aacccttcta cgggcttgcc actttgtgtg taggcgtggg gcagggagaa
     8521 acgctgatcg tggaatggct ggggtcctga gattccaaag ggtattttgt taacagcccc
     8581 cgggaagggt atcactagtc ggtcagctat cctgtataat acaataccat ctggtatcga
     8641 ctgttctcat aacaaaagtg agtggaacaa ggagaaaacg aatgcccctt tccggaacga
     8701 tcatagtaga tgaaaacctc tgcaaagcct gtgagctttg tatcgtgaat tgccccgtgc
     8761 aggtgattgc gctcgataag aaccgcatca cgccacgcgg gttccatccc gcggcgttgt
     8821 tcaaggaggg ctgcaccggc tgtggcacct gtgccgtcgt ctgtcctgaa gctgccatta
     8881 cggtttaccg cgaaaaacac gtcgaaaaag agcgcaacgc ttaggaggac atgatgagta
     8941 gagaattatg gaagggcaat gaagcgatcg ccgaagccgc cgtgagggca ggtttgcagg
     9001 catattttgg ataccccatc accccgcaga ccgaagcgtt ggaatacctg tcctggcgcc
     9061 tgccagaatt ggggcgcgct tttttgcagg cagaaagcga gttgggcgct atcaatatgg
     9121 tgtacggcgc agcctgtgcc ggagcacgca ccatgacgac cacctccagc cccggcactt
     9181 ccctgatgat ggaggggctt tcgtatatcg ccggcaccga ggtgcccatg gtgttggtgg
     9241 atgtggtacg cggcggtccg gggttgggca atatcgcccc cgcgcagggt gattacaacc
     9301 agatcgtgca tggcggcggg catggagatt atcaccccat cgttctggct ccagccagca
     9361 tccaggagac catcaatacg gtctatgaga ttttcgatat cgttgaaaaa tatcgcacca
     9421 tcggcatcgt gatggtggat ggcagcatcg gtcagatgat ggaaccggtc gccctgccgg
     9481 cgatgaaaga agtgcgcaag gtctggccct cttgggcagc atccgcaacg gaagggaaac
     9541 gtgaacgcaa cgttctcact tctatttatc tggatcccaa acaagaggag atcaccaacc
     9601 tgcgtctgat gaaaacctgg caggagatcg aaaagaacga gattcgctat aaagaatatt
     9661 tcatggaaga cgccaaattc gccgtggtcg ggttcggttc ggcaggtcgc gttgcccttt
     9721 cagccgtgcg cgccgcgcga gcagaaggca ttccggtggg tttgttcaga cccatcagtg
     9781 taagcccatt cccgaaaaaa cagattttcg acctggcgca aaggctagag gggtttttcg
     9841 tggtggaaat gaactccggc atgatgctgg acgatgtgct gctggcatct caggggcgca
     9901 ttccggtgga attttacggt cgcatgggcg gagtgatgcc tttcccagat gagatcttgc
     9961 gcgagattcg gcttttacac gctgatggct ggaaaaatga ggggcaccca cgcgaccgct
    10021 ggttggcaaa attagaaacg atcgtaaaag aggaatagca caatgacgac catgaatgtt
    10081 gaagatatgc aggtcatcta ccagcacccc acttctttgc tggatattca tacgcactat
    10141 tgccccggct gcacccatgg ggtcgctcac cgtctggtag cggaagtgat tgacgaaatg
    10201 aacctgcgcg atcagtgcat cggggtagct tcggttggct gctcggtctt tgcgtataat
    10261 tatttcgact tcgatttcat ccaatccccg cacggcaggg cgcctgccat ggcgaccggc
    10321 gttaaacgag tgcatcctgg taagatcgtt ctgacctatc agggagatgg cgatatgatc
    10381 tccatcggca ccggagagat cacgcatgca gccgctcgcg gcgagaacat aaccgtcatc
    10441 tttattaaca acgccaatta tggcatgacc ggaggacaga tggctcccac cacactgccc
    10501 ggtcaaaaga caacttcgtc acccaaaggt cgcgatgttg agcaagccgg atatcccatc
    10561 cgcgccagtg aaatgctggc tacgctggat ggcgccgggt acatcgcccg ccgcagcctg
    10621 cacgatccca agaacatccg cctggcgaaa aaagccatcc gcaccgcttt cgaggcacag
    10681 gtgcgcggtt tgggattctc catggtagag ctgctatcga cctgccctac caactggcat
    10741 ctggctcctg ctgctgccgg aaaatggctt gaagagaaca tgctcccgta tttcccactg
    10801 ggagactaca aagttcacac tgcgttgaag gacttgaaat tctaagagga gagaacacat
    10861 gcagaacgaa acactgattg ccggttttgg tggacaggga gtccttttcg cagggaaagt
    10921 gttagcacat gcagcacttg aactcggtct tgaagtcaca tggttccctt cgtacgggcc
    10981 tgaaatgcgc ggtggaaccg ccaactgcac ggtcatcttt tcagatgaag aaatcggatc
    11041 gccgcaggtg ctcaacccca ccagtattgt tgtaatgaac cagccttctc tggataaata
    11101 tgagaacctg gtgcgcaaag acggctacct ggtcgttgac acttccatga cgaaccgtgc
    11161 ggtggaacga aaagatatcc atgccatctg cctgcccgcc accgagagcg cagaagaaat
    11221 cggtgataag cggctggcaa atgtcgtgct gctcggcgct ctgattcgcg cttcaaacta
    11281 cttcgggcaa gcggagttag agaacgccct taagaagagc ctggaaggaa aaaaagaaga
    11341 attggtggct tcgaacatca tcgcattaca gcacggtttc gaatataata gttaaacgct
    11401 gtctggcgta tcttgtaggc ggtcacaaaa tatggtaaga taggcgacat tatacaatcc
    11461 cttttgactt agaatttaat ggcagatgca ctccatgggg agtcattttc tttccagaag
    11521 aaaaccaaga aaattctaag tcagcattat ttttaacgag gaaaaaacaa tggcaaacac
    11581 attcttgacc aaagaaggtt atcaaaagct gcaggaagag cttgattatc tgcgcggaac
    11641 tcgccgcgca gagatcgctg agcgcctgcg cgaagcagcc ttcggtgaag acctggttga
    11701 aaatgcggaa tatgaagcag ccaaaaacga gcaggctttc attgaaggtc ggatcaaaga
    11761 tctggaaggt ctgttagcaa ccgcgcgcat catcgaatcc accgccaaag ggagcgaagt
    11821 tgtccaactg ggttcaagag ttgtcattca agaatcgggc aacaaggatt ccgaagaata
    11881 ttttatcgtt ggcgctgcag aggcgatgcc ggcagagggc aaaatatcca acgaatctcc
    11941 cctggggaga gccttgttga accacaaaga aggcgatcag gtagaagtgc aggcgcccga
    12001 tgggtcattt aaggttaaaa taattgaagt cgcttaatgt cggagcatca taaaaagccc
    12061 cgctccgtta aggcaggcgg ggctttttca ttaaaaatag aggattgtgc acatggaatg
    12121 gaatgaactg gagaaaatac gcctgggcaa gatcgaccaa atgcgcgctc aaaacgtgga
    12181 accctacccc acccgcactg aagtagatca tgcgataaag caggctatcg atacgtttgc
    12241 gtccttcgaa aaacgaaatg atccttccat cagcgcacct gaagtaaccc tggcaggtcg
    12301 catccgatca aaccggaaca tgggaaaact ggctttcagc catatagaag atggaagcgg
    12361 gcgcatccaa cttatgctgc gtgtcaatga gatcggggaa gagggtctga aatccttcaa
    12421 cgatcttttc gatctgggag attttattca ggctagcggc agtctgatgc ggtcaaaaag
    12481 cggcgagatc accgtattgg tgaaggattt caaaatgctc gccaaagccg tcacccctct
    12541 gccagcagcc aaagacgagg tggtagatgg aaaaatcgtc aaacatgcca ccttcagcga
    12601 ccccgaaagc cgctaccgtc aacggtatgc agacctggcg gtcaacgaag aaatccgcaa
    12661 gatcttccgc atgcgcacag ccatcgtgcg cgccctgcaa aaattcctcg accagcacga
    12721 ttttattgaa gtggaaacgc ctattttgca gcccatctat ggcggtgcgg cagcccgacc
    12781 tttttccacc caccataacc aactccacca ggatcttttc ctgcgcatct cttttgagct
    12841 ttacctgaaa cgattgctgg tgggtggttt tgaccgtgtg tatgagatcg gtcgcgattt
    12901 ccgaaacgag ggcgtttcat tcaaacataa tccggaattc acccagcttg agttttacat
    12961 ggcatatgca gattacgaaa agatcatgag cctgaccgag caaatgctgg gatatgtctg
    13021 tgatgaggtt ttgggaacgc gtacgttttc ttttgacggg cacgagatca gcatgaacat
    13081 accctggaaa agggtagaac tgcgccaggg aatcctggat gctaccggcg ttgacatcta
    13141 tgcctgccct gacgcagata gcctttttca agcgatgaag aaaaaaggct tgaacccaaa
    13201 agaagggcag ccgcgtggaa aattgatcga tagcctgatt ggagattttc tggaaccaag
    13261 ctgcatccaa cctacttttt tgttcgatta cccgcgtgat atctctccgc tggcaaagaa
    13321 caagccggac aatccgcagg tggttgagcg ctttgaaggt ttcgtaggtg gaatggaact
    13381 ctgcaatgct ttcactgaat tgaacgaccc gctggagcaa gaaaaacggt ttgctgaaat
    13441 gggacgcacg tacgatgaaa aagacgaaga acgtcacccc atggatgagg attatttgaa
    13501 agccatggca tatggcatgc cgcccagcgg tgggtttgga atgggtatcg acaggcttac
    13561 catgctgctc accgacaacc gctccattcg cgaagtgatc ctgtttccgc acttgcgtga
    13621 aaccgaagag gaatgatcat aaaaaatccg cttatgtgga ttttttattc tctgacccag
    13681 gcgttctctt cacccaccag atgatgaacc ttcgccaaca ggtcatcggt gatacggata
    13741 gcgtcattcg gaaagtccat caccacatct cttccatttt cacgacacag aaaagtgaag
    13801 cggtcatctc ccggagaagc gcgcaacatg ccgtacacct gttgcaacag gcgaatatcc
    13861 tggtctggct tgccagtcgc attgatgatg acggttaaac tcttgccctt tgcattggta
    13921 acttgaggta gataaccagc ttcaccttca gacacgccct tttctatctt ttcaacggtc
    13981 agaggagcgg gttgttctcc ctcttgcgcc atgggttcat tctgcagcgg atccgtctcc
    14041 agcagcatgt cttgaggaag atcatcgaaa aatcctgctc cgcttcccat caactcctca
    14101 ctataggcat cttgaggcat caatccccat tcatcggtcg cggacttttg aagaaggtcc
    14161 tcttcatcat cagcgtttgt atcaatctcc aacttttgaa tatcatctac cagaaccttc
    14221 gcgtccatcc cttccacatc cgccttacct ctaacgatca ccacttcatc ttccaccagg
    14281 attgtctggg attttgccca ggtgcgcgga aagatcacca gcccaatcgc cccctgtaca
    14341 tcttcaagat tggcaaatcc catcggatca cccttttttg tgacgatgga acggatattg
    14401 gtcaacctgc ctgctacgac cacttcttct ttttgttggg cgtcttgcaa ttgccctgaa
    14461 taatgagtga cggagcgttt aatggttttc atgtaatcag aaagcgggtg gtcggtgagg
    14521 aacaatccca gcagttccct ttcccattcc agtttttcct tcttatccat ggggtcgagt
    14581 tcaggcaacc tgatctcttc tttcaacccc tccaccgctc caaaaaatga aagctgacct
    14641 tcctccttag cccggaaatg gctttcgctg atggctacca agcgatccat cacattaaag
    14701 atcgcccgcc gatcaccaaa ggcatccagt gcgcccaccc tgatcaaaca ttccaacgtt
    14761 cgcttgccca ctttccgcag gtcgacacgc cggataaaat catccaggtc ctcgaacggc
    14821 tgctctttcc gggcggcaac aatcaagtca gccggagcct gtccaacatt tttgatagcg
    14881 cccattccaa aacggataca gtagccattt ttctcctgat cctcgatgga aaaatcccag
    14941 aaactgtggt tcacatccgg cggcagcacc gagatgccca ttgccagaca atctgcgatg
    15001 taaaaagcaa ctttttcact atcattcatc gacccgcaca gcagcgcggt catatattcc
    15061 agaggatagt gggtttttag ataagcggtt tgtacggcta ccatgccgta atcagcggcg
    15121 tgacttttat tgaaaccata ctgggcaaag ttgcgccagt catcaaagat caggcgggcg
    15181 gtttcctcat caatgccgtt cttaaccgcg cccttgatga actttttttc atgaaccctg
    15241 atctgatctt cctttttttt cgaaatagcc ttgcgaagcg tgtcggagtc tgcgggcgaa
    15301 tatcctgcca gtgttatagc cgccgacatg atctgttcct gatagaccgc gatcccgaac
    15361 gtttcttcca tgattggctt caataattcg tgccggtact ccactttttg cttgccgtgc
    15421 atgcgggcaa tgtaatcagg gatgaattgc attggtcccg ggcggtacaa tgcgaccatc
    15481 gctatgatgt tgtccagatt tttgggcttc atctgcacca gatacttggt catgccgccg
    15541 ccttctagct gaaagacgcc cgccgtcaat cccttcccca agaattcaaa ggttcccgga
    15601 tcatccagcg ggatattgtc caaatgcaga tgcacgccat gccgcttttc gaccagcttg
    15661 cacgtattcg acatgatcgt caaggtcgcc agacccaaaa aatcaacctt cagcataccc
    15721 aagtcgtcca gaaccgacat ttcaaattgg gtgacagttt taatagggct atcctcagac
    15781 tcacgggtag ggcgatgcag tggcaggtat tccaccagtg gtttatcaga gatgaccacc
    15841 cccgccgcat gcgttccggc attccgtatc acgccatcca acttgccagc cgtgtcgatc
    15901 agctcatgca tgtgcggcac attcagatag atctctttga gttcaggaac ctcttccagc
    15961 gcttcttcga tggtgagcgg atgactgggg atgttgggta tcaacttagc aacacggtcc
    16021 acttctccca acggaatacc catcacgcgc cccacgtctc tgatagcctg gcgggctttc
    16081 atggtgccaa aggtgataat ctgcgccacc tggtcacttc cataccgttg tgcacagtac
    16141 tgcatcactt ctgccctttt atcatcctgg aagtcaaggt caatatcagg catgcttacg
    16201 cgggaaggat taagaaaacg ttcaaagatc aacttatgcg taagcggttc taccagggtg
    16261 atatccagcg tgtatgccac aatggaaccc gctgaggaac ccctggcttc gtaccatatg
    16321 tcattctctc gggcaaactt gcaaagatcc cacacgatta agaaataggc atcaaagccc
    16381 atttcatgaa tgaccttcag ttcataatcc agacgctcgc ggatttcggg ggcatcggca
    16441 cgttctttat agcgtttttg gagcccgtgc tcgcacagca tgcgcaaata actggctgaa
    16501 tcatatcctt ctggaacgtc gaatttgggc agatgatacg catcgcgctc caacttaacc
    16561 gcacaacgtt ctgcaatagc caccgtattg ctgatagact cgggcacttc agcgaagagc
    16621 gctgccatct cttgcggtga gcgtaaataa taggtgtcac cattcatatg catgcgttct
    16681 ttgtcggtaa gcaaggctcc ggtttgaatc gccagcagaa tatcctggta gcgggcatct
    16741 tcgcgatcta catagtgcac atcattggtc gccaccagat tgacctgata gcgctttgcc
    16801 cattcaatga gttgcttatt gaccgtcaca agttcggaca tattgtgatc ttgcagctca
    16861 aggtaaaaat tttcttttcc aaacacatcc agataccagc gcaattgttc ttccgccagt
    16921 tctaactggc tgttcatgat gagagaggga atttctcctt ttaaacaagc gctggtgcaa
    16981 ataatccctt cggcatgatc agcaagatat tcgtggtcga tacgcggata ataatagaat
    17041 ccttccagtt gagcagcgct ggctattttg agcaggttct gatagccaac catgttttca
    17101 gccagcagta agatatgatt cgagcgttta tctttttgtg gatcacgctg gtccatgcgg
    17161 cggggggtga tatacccttc cagcccaatg acgggatgga tgccttcttt caggcaggca
    17221 ttatagaact ccaccacccc atacattgtg ccatgatcgg tgagcgccac ggctggcatc
    17281 cccatttctt tggcacgttg aatgagcttg ggaaacatac agtaaccatc aagcagagag
    17341 taatgtgagt ggacgtgcag gtgaacaaaa gacatagcgt taaaaaagga tgatttcggt
    17401 cattatagca cgcgaccaaa aatcatccat tccaaccacc ttaaaaatag aatgatttta
    17461 aggttagcgc atcacgccac ctttcccagc gtggcaagat atgctgcgaa ttgatgcgtt
    17521 ccatcaagcc ccaatgaggc gttcagcaga tcatcgtcaa aagctgcgat agcacatact
    17581 ccgcaacgga tagattcagc cgtcagatag agattctggc aaacatgccc ggcatccaac
    17641 agaatatacc ggaatgcgcg ttcactataa cgccagtaag ttcgcgcgat gtcagccacc
    17701 cagcagaaag taaccgcgct gttctgcaca tgcttttgtt tcaaacacgc atctgtgatc
    17761 ctttggttca catcagtgcc atcatccagg agcaataact tgtgttcgat cgccagatag
    17821 cggtacagcc cctggtccaa attctccacg ctgttaacga gcaaatacgt ttcaaaggga
    17881 tggcgcgatc cggctgaggg aaccatcctc ttggtgaacg ttccgctttc gttcaccgct
    17941 ttcacaccct gggtaaccca aagcagatag ctcagttcca ccagcgatag cctgtcggcg
    18001 atgtaggatc tccggctttc tcgcgtttcg gagatggagc ggaaatcgga acaggcgagt
    18061 gcacatcctt tcggatcagg cagcaaaatc aaaggtgcag cggggtcata tgccatctgc
    18121 aggggtggct gaggttgccc tactttttgt tgcgattcgg gcatgttttg atatttggat
    18181 gcttcgaaat aatctttcca ggtgctcatc atctctcctt tgtcaatgaa ctgtcttttg
    18241 cctgcttagt ttgcccagga ccaaatgcaa caaggtcttt atttcttcta ctttaagaaa
    18301 atacagaacc gatgcataaa gaccggcagc caccagaatg gtcagcgcaa tatgcaacca
    18361 atagggtaat gccacatcca gatccatgct gacagtcaca caaaaagcca tgatcaaaac
    18421 cgccaagccg gcttttagaa gtccggagag cagcagtttt ttatccagcc cacgcatccg
    18481 ttttttcata agcgaaagca atacgagggc ttccagcaaa gtggcagtgg aatttgccag
    18541 agccaaaccg ccatgcggca tccacccaat ggatgaaaag agttggctga aaaggatact
    18601 taagaccaaa ttcaaactca tggcagccac cccaacaaac acgggggttt tcgtgtcatg
    18661 ctgcgcataa aaagcgcggc tggtgatttc cacaatgcag tgcccgatca gaccaagcgc
    18721 ataccagagc agagcccagg cgaccaaatt cgtggacgcg gcagtgaact cccccctctg
    18781 gtagagcagc cgcacgatat cttcacgcag catgatcaaa ccgaccatag ctgggattgc
    18841 aagaaagagg atcatgcgca atgtggaggt aaaggacgaa cgcatctcat ccaatttccc
    18901 tctggcaatc tgggcagaaa aagtgggcag cgaagcgatg gcaatcgact gcgcaatggc
    18961 agcctgcggc atcaacatca acgaaaaacc caggctgatg gcagtcacac tgccttcggg
    19021 ttgcagggat gccagatacg tattcaccag aaagttcaat tgcacaatgg cgacacccaa
    19081 caagcgggga gccattaaaa tggcaacttc ccgcacggcg ggattcttcc tttcaagccc
    19141 cggtttatac cgcacctgcg gcagccgcag caagtttgga acctgcacca ggagatgaaa
    19201 gccggcaccg atcaagatgc cataggataa cccatgaacc ccgaaacggg gtgccagcac
    19261 cagggcgcca aagatccagc cgacctggta catgcccggt gccagagcgg agacaaaaaa
    19321 tcgctggtgc gcgtttagaa tccccatcac caatccactt aacccaaaaa ggatggcaga
    19381 aggcacctga atgcgcagca gatcagcggc taacatttct ttttccatgc tggcagacga
    19441 aaaaccaggc gcaaggatgt aatgcaccac ctgccgtgca aagatcatgg ttagaatgct
    19501 gaaaatggtc agaatgagca gtgcccagtt gatgatcccg ctggctaatt tccaggcgcc
    19561 ttcgcgatca tccttcacca gaaacccggt gaaagtggga ataaaagcgg aacccaacgc
    19621 cccgcctgct accaggttga acaaaatctc cgaaaaacgg ttggcagcat agaacgcctc
    19681 gctatcggct ccggttccga aagcgcgagc ggtaaaagct ttggcgagca accctgccag
    19741 attgctgaga atataggcaa gcatgaccgt gccggctgct cttgcgatct gccggttagc
    19801 ttgatggact gtatcgtgct cttggggcat gctgttgatt atactcaaag gtttcgtttt
    19861 cataccaaac tcatatctgc cagcgataaa agcatagtaa tataatttta ataacgctat
    19921 ctgaaaggaa caacccatgg aacagaaaaa gggaaatacc accacctcca acgctgcacc
    19981 acaggtcaac gctgggtcac aaaaatcgca gcaaccgccc acagccaagg tggaaggaaa
    20041 agcgggagta aaagtccata agaattcctg gacacaaaaa gccctgcgcc aattcttgtt
    20101 ggcgatcgtg ttcctcgcca tcggtgccgg tctgatcttt ttcctgatcc accaacccgc
    20161 tgcgaaggaa tatgttgcac aaaaagagga agccacccgg ctgcaggata tcgaaagcca
    20221 atacaaccaa ctgctgaccg attacgccga ccttgacaca cgccataagg tgtcggtaac
    20281 actggtcaat atctacaaaa tccagaacaa cgtcaatatc gcccgcatcg ctttgatgaa
    20341 tgacaatgaa acgcgtttat ccatcgccct gggatatgtg gaaaaggata ttgcaaccct
    20401 ggatattggg gaatttcctg aaaaggtgga tctctcggca cgtgtgacag ccatcaaggg
    20461 ctatctgcca gatgagaaac aaaacgcctt gaatgaattg aacgctctct ttgatgatct
    20521 tcttcttctg gcaaacaata tggagatcac cgaataaaaa aagagctcct ctcccttcaa
    20581 cttatcaggc gaggagctct ttttctcatt gaccagaacg tgagccgaga gtcacagaga
    20641 tatctctttc agcgccattg cgcaacacgg ttaccaccat ggtgtcaccg ggggctttat
    20701 tcatgaacag gtaggtcaac agttgattgt agttcttcgt ggttacccca tccacagcca
    20761 cgatcaggtc accgccggca tacaagccct gatattttgt ggctcgggtt cctgcccgaa
    20821 taccagctct ggcagccgga ccgcccggtg tgacatccag aacgtacacg cccggaacat
    20881 tggggtcgaa acccatggct tcaatctcgt ccatggtcaa cccatccacg cttgagatac
    20941 ccagatacgg ataatcataa gagccttcac tgatcagaga gggcaccacc tttttaacca
    21001 tgtcaatagg aatagcaaag cctattccag aatatgaagc gctttcataa atgctggatg
    21061 tttgatcata gatcgccctg ttgatcccaa tcacttcgcc ttccaggttc agcagaggac
    21121 cgccggagtt tccagggttg atggctgcgt cggtttggat ggtatctcct gctccaaagg
    21181 gagagccgct tccgctggaa cgtattgagt cgagcacacg ccccatagcc gagatgatgc
    21241 cagtggtcat cgttccgcta aacccgtatg gattgccaat ggcgatcaca aactgcccca
    21301 cttcgagctc ggaggagttg cccaggataa gcgggtgaag ctcttctgcc agcatatcca
    21361 ctttgaccac tgcaatgtcc gaatccgtgt cggtgccaat taccctgcca cgggtgcgcg
    21421 ctccatctga aaaagtgatc tcgatctcag cggcattttc aatgacatga taattggtga
    21481 tgatgtgccc ttccaggtca tagacaaaac cagaaccctg ccctgcacca ccattggtcg
    21541 tgtaaaccac aatagaaacc acacccgggt tgacactctt gtaaatgttg accagcatat
    21601 cttcaccctg ggtgtattcc aacacctggt caggaacagc atctcttcct tcttcggttg
    21661 ccgtagtaat ttcaacagga agctcttgca tttcctgaac aacaccgcag gagctcaaga
    21721 tcagtcctgc aaagaccaaa cttgaaaata aaatttttct gctcttaatc atttgtcctc
    21781 cttcttccac aaatctcgca aagttgatcg ctgtttttct gtgagattgc gcgggatctc
    21841 cactttcact ttcaccatca ggtctccaaa cttgtctttc tgtttcaacg aaggcaaccc
    21901 atgccccttg atcctgaaga tttgcatggg ttgcgttccg ggagggatct tcagcagaac
    21961 atccccagag ggtgtgtgta ccttctcctc cccgccaagc agggcggtgc caacatctat
    22021 tgtgatggta gtagaaatat catcgccatc aacggcgaac tgtttatccg gagcgagggt
    22081 gatgaccagg taaatatctg catgggtgcc gtccgcttgt cgcggaccag caccaacaat
    22141 gcgcactttg ctgccagttc tcacccctgc cggtattttc acctcgatct ttttatcact
    22201 aaattgtaag agccggctgg ttccatgata ggcttcccaa aatgagatgg ttaccggttg
    22261 ctgatacgct tgaggacgag ccgaccgtcc tgcggatggg gcttgcgcgt tcctgcgctg
    22321 gctgcgtgat cggggggtgc caaagaaagc attaaagaag tccgaaaaac caccaagccc
    22381 accgccaaac agatcctcaa aatcatctac attattgaca cgggttgtgc gggtggtata
    22441 ttgcgggttg ttgaacaggt cttcccagcg gaaggtattc ggattattcc ccgccctctg
    22501 ccacgactga taagaattcg acaactgatc atagcgcgac cgtttcttgg ggtcgctcaa
    22561 aacctcattg gcttcgttga tctctttaaa acgttcttcg gcttgcttat tccccggatt
    22621 tttatcggga tgatatttca tagcaagctt gcgataggct tttttgatct cgtctcctgt
    22681 agcgtctttg ccaacgccca ggattgcgta ataatcctta tactccatgt tggctattgt
    22741 atgattgtgg gagaatgaat gcaagagact tcagacagat ttaatattat tctaaccata
    22801 tccaaatctg ctatttaaac aagcatccgc tggcagggag gattgccagc ggacactaat
    22861 gccaaaggag gagacattga tgagtattat ctgaggtgct tgtaacccaa catgtcaccg
    22921 cgagaaatgt taccctttgg gaagattgta tgcaacgatg aaagtcatat tttgtttatg
    22981 atcttcttcc attaaacata atcgtccaga aattcgtcca acactttcaa caaatcatcg
    23041 atcccctcat aggcataaac acagaagatc ggattctcgt ttcccaggat catctttgac
    23101 actttttttc cttttcgata gcagcagaag accggctttt tattcatttc agccagggct
    23161 atttcatatc ccaccccatg agatggggtg ctgacctcgg cgatcaggag atcactctcc
    23221 aacacccaac gcacatcccg ttcataaacc acgcgcgggt cgatcatccc ttcttcatcc
    23281 ggcactccac tttctgccag atgcgctgtc ggcaccctcc attttcgctc aatgagatga
    23341 tcaacgatct gctggtaaat gtgttgttcc gtcctaccgc cggtaatgga acaggagaag
    23401 tatgcctgca tgagccctcc atggttcgat tatttaccat ctgttgttaa tgtaccacga
    23461 aatcggcgtg ttaaaattag ggtatgacat ccgccaaaga acatatagag aacatcctcg
    23521 ataccttacc cacaaaaccg ggttgctatt taatgaaaga cagcagcggg aagatcatct
    23581 atgtagggaa agccatcaac ctgcacaacc gggtgcgatc gtactttcaa tctgcagcag
    23641 accattcgct aaaaacccag cacctggtgc gcgatatcgt cgacatcgat tggatcgtgg
    23701 ttggctcgga gctcgaagcg ctgatcctcg agatgaatct gatcaaaaaa caccgacccc
    23761 catataacgt acgcctgaag gacgacaaac gctatcccta tatcaaggca cactggaatg
    23821 aacctttccc gaaggtcacg gtaacccgca acctggaaaa cgatggcggc aagtactacg
    23881 gtccctatac aagcgtctgg gcggtacatc aaaccctcga cctcttgcgc aagatctttc
    23941 aatatcgcac ctgcgaccgg atcatcaccg gaaaagaccc gcgcgcatgc ttatattatg
    24001 acatcaagct gtgcagtgcg ccctgcatcg gtaagatcaa cgagagcgat tacaaccaaa
    24061 tgatcactga tctgggaaaa tttctagaag ggcagaccga ccccatcctc gaccgcctgc
    24121 gcacgaggat ggaggaaatg gcagaaaccc tccaattcga aaaagccgca gcgctacgag
    24181 accaagtcca ggctatcgag cgggtggtgg aaaagcagaa ggtcatctcc acccagaaca
    24241 tggacagcga cgtgatcgct ttggcgcgct ccaatggaga agcctgcgta caaattttct
    24301 tcgttcgcgc cggaaagctg atcggacgcg attatttcat tctggaaggg gcggatgatg
    24361 aaaaggacag cgaggtcatt gcccaattcc tgacccaatt ctattcccga tccgcacaca
    24421 ttccatctga agtgctgctg cctgaggaca tcgaagaagc catgatcatc cagaactggt
    24481 tgaatcagaa gaaaaaggac gaaaagatcc agatccgggt tcctcgtaaa ggggtgccca
    24541 agcaactggt tgaaatggct acagaaaatg ctatggagac actgcgttca ttaaagttac
    24601 agtgggagat ggacacgaac aaacaaagcg tcgctttagc tgagctgcag gcagcttttg
    24661 gaatgaacaa tccgcccaac cgcatggaat gttacgacat ttccaacacg caaggcaccg
    24721 cttcggttgg cagcatggta gtctttgagc aaggcgtccc caataaaaag cattaccggc
    24781 gtttcaatat caagacggtg aaccaacccg acgattttgc cagcatggaa gaggttctca
    24841 cgcgccgctt tcgccgctgg caagcatacc acgagagcga tgaggtcgga aagaaagcgg
    24901 aaccgtcctt ttcgatactg cccgatctgc tcattgtgga tggcggaaaa gggcaacttg
    24961 gcagagcttt aaaagtgctg gagaagttcg gcttgcagaa ttccatcaag gcggtaggat
    25021 tggctaagag tgaagagctg gtgatcctcc cagatcaggc ggaacccctt gccctgccaa
    25081 ggaattcaca ggggcttttc ttgatgcagc gcattcgcga tgaagcccat cgctttgcca
    25141 tcaccgcgca tcgcaatcaa cgcagcaaga acagtttaag gtctatcttg gatgaaataa
    25201 cggggatcgg agcgacaaga aaaaaagcac tgctcgctga attcggttcg gttgaaaatc
    25261 tgcgccaggc ttcggtggaa gatattgcca agttacccgg catcagccgc tcgctggcag
    25321 aggagatttt gcgttcaatt caatagagca gtgctcacat agaaaaggtt ctttactgtt
    25381 gtaaataatc ctggaaatag ttcaataaaa ggaccggttc gctgtcctcg aaccattcaa
    25441 gataatcgca gaaaaagttc acgtccaatt gagagtcaaa gggaagaacc gtctcaagcc
    25501 agaaatcgcc ctccgcatcc acggagacct ttgccagaaa ctgctcatca ttagcctggt
    25561 tcactgctga gatgacttcg ggttccagat tgctggtgcc tgccccaatc caaagcgagt
    25621 aaataatcat gcggctgatc ttctcatttt gataatcata ttcgatcaga tagatgccac
    25681 cgctgggggt cgttatgccg aaagtacttc cttccacatt gctcaccgtc cagcccttcg
    25741 cggtaagttt ttcggtgagc acgagaacga atgcgttttc gtctgattgg gctgcgacaa
    25801 aatcggcagg tgcttcttcc gccacagctt gatcggcgcc ggataacact gctacctgtg
    25861 tttgtaacgc gttgacttcg gctgtattat tcgccgtaca accgtataaa aataatacca
    25921 agaggataaa aagtaatttt ttcatgttca cctgtttttc tgttcattgt atcagaaaca
    25981 aatcgaacgc attcatcatt gaatcatgcg tgcaaacacc gcacaatgtt ccttgcatgc
    26041 ccggtgcttt ctatggtaga attcaacgcc gtttggataa aaaaagcaat tattcattaa
    26101 ttgataggaa agcaaagtca tgagcgaatt attgaaagca gtcgaaaccg aaaagaatcc
    26161 gaacattccc gaacttgctc cgggcgatgt ggtcagcgtg catgtcaaga tcaaggaagg
    26221 cgatcgagaa agaattcagg aattcaaggg aaccgttttg ttcgtttcaa tgagcgggca
    26281 atccagcacc tttaccgtac ggcgtgttgc cagcaatggc ataggcgttg aaaggacctt
    26341 cctattccgg tcaccgaaga tcgacaaagt ggtcgtagaa cgccataaca aagtgcgcaa
    26401 agcgcgcctg ttctacctgc gcgagcgcac cggcaaacga gcgcgcctca aacaaaaatt
    26461 ttcatagatt ttccacctgc gagtacgaaa aggtgcagtc tatagaactg cacttttttc
    26521 atatataatc tccttcaatg atgaaccgta caagttcaaa gtcgttgaaa gtcggggtct
    26581 tcgattcagg ggtgggcggg ctttccgttc tgcgatctat ccatgatcga ttgccacacc
    26641 cggagatcgt tttttttgct gaccaggcgc acgtacccta tgggcgccgc cagttggatg
    26701 agatccgttc attctcacaa caaattacgc tgtttttgct ggaaagagat atcgatctgc
    26761 tggtagtagc ttgcaacacc gcttcagcgg ctgcattgaa atttctgcgc cagcagttcc
    26821 cccgtctgcc aattgtcggc atggaaccag cggtaaaacc tgcatcagag caaacccgca
    26881 ccagggcagt gggggtgctg gctacccctg ccacttttca gggcgaactg taccaatcgc
    26941 tggtccacaa atatggggaa gggcttcaga tataccagga tacctgcccc ggtctggtgg
    27001 aggagatcga agccggggac ctgacaggtg aaaaaacgcg ttccattttg gaaaacgcgc
    27061 tgcttccaat gattgagctg aatgtggata cgatcgtatt gggctgtacc cattacccct
    27121 tcgtcattga cacgatgcga agaatcgcgg gaaaacatat ccaggtgatc gacccggctc
    27181 cggcagtagc acgcagagtg caggctctgc tcaaccagca attcccggag cgcatgccaa
    27241 tagcttcctc tggatccacg cagttcttta ccagcggcga tccgctcgtt cttcaaaagc
    27301 agatcaagct gctgatcggt ctagaaacca ccgttcgctc cgctcactgg gaagatggca
    27361 aggtttatgc tgactgatcc gaacaggaaa gcaattttga ttcaaactac aagaggaaat
    27421 tatgagatca atcgcaatca tcatggctgg ttttggaaat gtaggcaagg cgtttgccag
    27481 gctggtatat cgcaaatacg cagaaatcga aaacgcatac gggttcgacc tgcgcatcac
    27541 gggtgtcagc accggaaagc acggctgcct tgggatggag accggtttcg atctcgaaca
    27601 gctagcttct ggggtccttt catccagctc gcttcctggc agcactccat tccgtgatac
    27661 gctggaaatg atcgaaaaaa gcgaagcgga tgttttcatt gaaaacactc ccgtcaacta
    27721 tgaaagcggg cagccagcgg tcgatttttt aacccacgct ttggaaaaag ctatgcacac
    27781 tgtcacagcc aacaaggggc cggtggtgca cgcttatgaa aagctcagcg atttggctcg
    27841 gcaaaaaggg aaacgcttcc ttttcgagtc caccgtcatg gatggtgcgc cggttttctc
    27901 ggtcgtccgc gaagcgctgc ccgccatgca gatcgccggt tttgaaggaa tcctcaattc
    27961 gtgtacgaac ctcatccttt gccgcatgga agagggggaa acttttgaac aggctgttcg
    28021 atatgcccaa tcgatcggca tcgcagaaac cgatcccagc ggggatatcg acggatggga
    28081 tgcagccgta aaggtagctg cgctctcgac cgtgttgttg aatcatcctg ttacccccca
    28141 acaggtccag cgcaacggta ttcgcgagat cagcgcagcg gatatgcgct ccgcgcgcgc
    28201 agccgagaaa cgctggaaat tgatctgttc agcacaaaag acaaccggtg ggtgggattt
    28261 atcggtcgcc cctcacatgg taggagctga gagcccattc ttcagcgtga acggcaccag
    28321 ctcgtatatt ctgttcaaat cggacgtgct gcccggtctg ggtctcctgg aatccgaccc
    28381 cagcccagac accaccgcct atggcatgct ggcagatatt ctgaacatct tcaagcaaga
    28441 cagggtagta tgaagcacca tgtgttcctc ggtatcggca ccaatctggg agatcgcccg
    28501 gcaaatctac aaaaagccat tgccactctt cagcgggtca tccaggttga gcagacatcc
    28561 aaagtgtatg aaacaccccc gtggggcttt gcggatcagc ccgcttttct gaatcaggtc
    28621 ctctccgcaa ccacacgtct gggtcccttt gagttgctcg ctgagatcaa aaccatagaa
    28681 accgagatcg gtcgcacccc taccttccgc tacggtccgc gcctcattga catcgatatt
    28741 ctctttttcg atgatctggt tctggacgaa gaacgcctga ccatccccca cccgcacctc
    28801 accgaacgcg catttgtcat ggtcccgctg aacgaaattg ctcctcaaca cgtacatccg
    28861 cggctgcaac gcaccattca agacctgatg ccagaagtgg acatcagcaa cgttaaactg
    28921 tatgaaaatt catccccatt ggagaaataa aaatggaagc gaaatccatg ttgccttttg
    28981 gaacgcgcac ttttgtgatg ggcatcatca acctcactcc cgatagcttc tcaggggacg
    29041 gattaatgaa aagctccgct atggcagaag cagcggtaca gcaagccata cggtttaccg
    29101 agcagggtgc agatatcctt gacctgggag ctgaaagcac acgccccggc gctgaaacgc
    29161 tggatgccag cagtgagctg gagcgtcttt tgccagccct ggaacgcatt gtagccctga
    29221 agctcaatac catcatttcc attgacacat acaaagcatc cgtagcagaa gcctgcttgc
    29281 aagcaggcgc cggcatgatc aatgatatct gggggttgaa acacgacccg caattagccg
    29341 gggtggcagc ccgccacgca gttccggtag ttttgatgca taatcgcagt catgctaaat
    29401 tcctgcaaaa gtctgaaact ttgggtgggc ggtatgtagg caccacctac caggacctga
    29461 tgaatgacat ttgcagtgac ctgcaagaaa gtatcaccat cgcaaagacc gccgctatcc
    29521 aagaccagaa catcatcctg gatccgggga taggctttgg gaaaacggtc gaacaaaacc
    29581 tgagcatcat ccatcatctg ggcgaacttc gcaggttggg ctaccccatc ctcttgggcg
    29641 cctcccgaaa atcgttcatc ggctatacgc tggaccttcc ggtggaagat cgcctggaag
    29701 ggacggcagc ggtcgtggcg ttggggatca acaatggcgc agatatcatc cgcgtacatg
    29761 acgtgcaagc gatggtgcgg gttgccagaa tgtgcgatgc cattctgcgc aattaaatac
    29821 gcgcctggtc cttcacattt ctctgttcaa tgatattttt ggcttgtttc aatcgaaagt
    29881 gatcggcacg ttcccgttca tccaattcat cccgaatgta agcatattca ccctgtaatc
    29941 gcgggatcac gataagttcc agtgctttga gccgccgcgt ggtatgtgca atttccttta
    30001 aaagagctgt tagctgcact tcctcatcag ccaatttcaa aatatcagcc acgttattgc
    30061 gaaaggcaac tccggcttct tcgatcatgc tggagatcga gttgaaaaca tagggctgag
    30121 tggattcttt ctcttcatcc cgtttctgtc ggatcttggg aacgcgcaca cccatcacga
    30181 tctcttcatc cagttcgatg gaatattcac tctgagctcc cagagatgcg gctttgacgc
    30241 gaccctctcc cacaaaagct tccgctctga ccaaagccat tttggcttga ttcgtggata
    30301 gttgcagccg ttgcgcctct tccaagactt tcctttccaa ttctacgatc ttttggatga
    30361 gagcaatgcg tttcttgtca agcaattcat acccctggcg ggtcaaaccc aattgttgtt
    30421 tggttgccaa aagctgattg cgcgttaccg taatattgga aacgtcgatc atgcccgttc
    30481 acccctataa tacttttcga tcagagcctc agaaatacgt ttcaaggatt ctttgggaaa
    30541 ttcagaaagc aattcccagg caaggtcgag cgtctgctcg atgctgcgat tttcattccc
    30601 ctgattcacg aacttctgct caaagttctt tgagaaattc aagatccgtt gatcttgttc
    30661 ggtcaaagac gcttcaccga tgatggcgac caacttctcc agttcacgcc cttctgcata
    30721 aaaagaatag atctgatctg ccaccgattg atgatcttca cgggtatggc cttctccaat
    30781 agcctctttc atcaatcgag acaatgaggg aagcacatcc acaggaggaa aaatgccctt
    30841 ctggttgaga gtcctgccca gaacgatctg cccttcggtg atatagccgg tcaggtctgg
    30901 gatgggatgc gtgatatcgt catcgggcat cgaaagcaag attagctgag taaccgaacc
    30961 ctgtttccct ttgatacgcc ccgccctctc atacaggctg gccagatccg aatacatata
    31021 ccccggatac cctcgccgcc cgggcagctc ttcgcgtgcc acggataatt ctcgtaatgc
    31081 ctcacaataa ttggtcatat cggtgaggat aacgagcaca tgcaagttat gggtgtaagc
    31141 caaatattca gccgcggtga gcgctacacg gggggtcatg atgcgctcga tggtcgggtc
    31201 atccgcatga ttgagaaaca cgatggtacg gttcagagca ccactggagc gaaaatgttc
    31261 aatgaaaaaa gaggattcgc gatgtgtaat gccgatcgca gcaaatacca ccgcaaaggg
    31321 ttcatcgctt tccttctttt cagtgagcac gcgggcttgc gaagcaattt gcgctgccaa
    31381 ttcattcccg ggcagccctg aaccggaaaa aatgggcaat ttttgaccac gcgccaacga
    31441 attcaaccca tcgatcgtgg aaattccagt ctgaatgaac tcctgcggtt cgtttcgaac
    31501 agccggattg atcggcgacc cgttgatatc ttcaagtttt tccgggacaa tggcaggacc
    31561 gccatcaatg ggttccccca taccattgag gatacgcccc agcatcgaaa gggatacctt
    31621 caggcgaacc acatcaccag aaaaacgaac gccggtgcgc gaaatatcca acccttctgt
    31681 acccagaaac atctggataa ctgccatgtc gcccgcaatt tccagcacac gccccatgcg
    31741 aacatttcca tcgggatcaa taatttgaac catctcatcg tatgccacac ccgccacgcg
    31801 ttctacaaag attaaaggac ctttgatctg ggagagtgtg cggtattcct ttgtagccag
    31861 tttggtattc attaatttct caggcattct ctgtctccca gcttgaaacg agtgcatcaa
    31921 aagtgtcatg gatctttccg gtcagtcttt tcaattccgc agtagaacgc tccaaaggta
    31981 tttctttcat acgcgcaatc cgttgcagca ctgtcagatt ggttatttca gaaaagtcga
    32041 tccctgtctt caaggctgct tgagctcttt gatgataagt gaggatgacc ttcagcatcc
    32101 aataggattt ttccaaactg caataggtat cgacgtcttg aatagatgat tgctgcaaat
    32161 aatcttcgcg cagcaacttc gaaatggcga ggatctcacg ctccacatca gaaagagcgt
    32221 cctgcccgat cagttgtacc acctgcagca attcattctc tcgccccagg agagactttg
    32281 cctcctgcac caattgggga aaatcttctt ctaccgtatc actgaaccag cgcgaaagat
    32341 caaccaatga aaaacttttt atccaattga tagctggaaa atggcgcttg cgcgacagtt
    32401 cataatcgag cgcccaaaaa acccctgcga tgcgcatgga gctttgtgtg atcggatctg
    32461 aaaaatcacc gccggggggt gaaaccgctc caaccagcga aaccgaacct tcacgatgtc
    32521 cggcataggg tttattttct ccattaccga gacaatccac gtgacctgcc cgttcataga
    32581 attcagccag acgggatgat agataggctg gatacccctc ttcaccgggt atttcttcca
    32641 ggcgtcctga aacttcgcgc aatgcttcac cccaacgcga ggtggagtct gccagcataa
    32701 gcacatcgta acccatatcg cgatagtatt cagcaacggt aataccaaga taaatgctgg
    32761 cttcacgggc agccaccggc atattggacg tatttgcaat aagaactgtg cggttgatga
    32821 gtggaatgcc tttcacccga tcttccaatt caggaagttg catcaacact tcagccattt
    32881 cattaccgcg ttccccgcac cccacatata ccaccacatc cacatctgcc cagcgagcca
    32941 gggattgctg aattacagtc ttgccggttc cgaatccacc cggaatgatg gcagaacccc
    33001 ctttcggaat cggaaagaaa gcatcgatca ccctttttcc ggtgaccagg ggctcagcca
    33061 ggtcaaggcg ccgtttatag gggcgggcac tgcgaactgg ccaaacgtgt tccatcttga
    33121 catccgtgat cctccctttt ccgtcctcaa tcaccactac cggttgcgtc agggtcaatt
    33181 cgccatcgtg cacttcaacc accctgccgt ttacagaaaa aggaaccatc acccgatgga
    33241 gaatactttg tgtttcaggc acttccccca atagattgcc ggattttacc aggtcacctt
    33301 cttttaccag agcggtgaac ttccattttt tatcctgcgg taacgaatct tcggtaactc
    33361 cctttttcag gaaatcaccc tcgatatcag ccagtttatt caacggacgc tgtaatccat
    33421 caaagatctg acccatcaat cctggaccca gatgagcgat cagaggatga cctgtgccag
    33481 cgattggctc tcccatccgc aaaccggaag tttcttcata tacctgaatg gtcgcctgct
    33541 gacctgacat gcggatcact tctcccacca gttttgcttc accgaccaat accatgtcgt
    33601 gcagtcggat tccctctaag ccgctcacgc cgaccaccgg tcctgaaatg cgattgatcc
    33661 tgcccgtctc tttaatcatc gtttccattc tcatcacctt tcaaatggaa ttgcgtacct
    33721 gttgcatgtc gaaccatctt ggaaagatat tcctgactgg ttttcttgtt cattttccct
    33781 gttcttcccg gaattgttac cagtgccagg tttttacagt tgcgcaaagc tgccactgtt
    33841 ttttgatcca aatgtgagaa cagatcatca tcaatagcca gcaataaggg ctcacttttt
    33901 tcaatgaatt cttccagcaa ttccgccagc gctgtagaat cggtaacacc atacgcttcc
    33961 aatcctgcca agcggtatcc atttgccata ctgggaatag tgatcacgac cagcttattc
    34021 attgcaagat ctccaaatgt tcaatgatgt ccacagagtc aaagcccagt tgtatccctt
    34081 ttgcaatcca ccagagattc tgcatttcat tctgtttccg aaccatgtac ccaatgggaa
    34141 ctccgatacc gatcggatct ttcaacgcat accgggaagc ccatgccaga agttgcttcc
    34201 gtaatcgctc ttcgaattca gccagcaagc cgctctttcc atacgcaagc aacccatcct
    34261 gtaaactggc aaagtatctt gatcttgtaa aatgggagac agctcgtgaa atatcgcttt
    34321 catttgccac agatatgagg aaagcacgct caaaagatcc tgcctgcagg aaacattcct
    34381 tgattgccaa cccgttgcgg atgagcatat ctggattttg aacgatacgc aatgcgcaaa
    34441 tgatattttc tcgatcggca ttcatatcga aatattcctt gagaagtttg tttcgattga
    34501 gaatgtgttt tttgcgggaa aggtatcttt cgtagaattc cttttcaagc aataattcga
    34561 tctgcgctcc ttgcatggta aaaagtgaag tttggttttt caataatgca tcggtataag
    34621 gcagatgaaa agcgatcatt ttagaaatca gatcactcaa attcctgctt cgggcaagtt
    34681 cctgaaggat gtgcattgga acagtaccag accggtttag gagttcctgg atctcacgat
    34741 aaggaacact tcccagaaca cctcgaacaa tagtatggag attctgcaga tcattatgag
    34801 aaaagatcaa gccgatcaga ttccaggaag attcatgata gaaatttcga taatcgagga
    34861 atagtttctc catctcgaat tcaatgatgt tatgcaccgc ttgaattccg ctggaaactg
    34921 tgagcgcctt ttcgatggac ggtttatagg agctcttgat taacaatgaa agatatccgt
    34981 ccagattatc tgcacgggtc atttcttcca gcatctcttt catgattaat tggggaaaac
    35041 gggctcgcaa gcgcgtgttc ccataatcaa aggtcgacat taatccatcc cagtcacttg
    35101 ctcactgaag aacaaagata accactgctg taagatcgga actgcctttt gaaaacgatc
    35161 gttgagtgtg ttatacacgc acaccttgcc gtccaggcta gaagcattac atcctcccca
    35221 gcaatccagg tcgtattgga aatggatttt tacagatcca tctttcggaa acttattcag
    35281 caattcctta tcttttggat caacagaaac aatgatctct ttctcctgcc ccagtgatgg
    35341 ttgcaaatct ccaaccgctt cttttatcaa ggagcgtaaa agagctggat attcaccttg
    35401 tgtgcgaatt gatcgcaagg ctatctcgat ctgtcctaaa gattcctgga tcagcttctg
    35461 gcgggcatca gagatatttt gtagatactg catttctgct tgttgctgaa tgatagcagc
    35521 ctcacggttc aagcggactc gcccctcttt ttccagattc ttacggatct cctctacttc
    35581 ttcttttgat cggaatagaa tctggttaac ctgttggacg gtttgctgct gtagctccgc
    35641 gatcttgttt tccgtgatcg cattgattcc agccagaatg gcttcgattc ccataaaaac
    35701 agtctccgct catcccatca aaataatggc agccactgca aaacccagta ttaccattgt
    35761 ttcagggatc gcgaccagaa tgatgatcga acccaataac tcaggttttt cagctaccgc
    35821 tccaacaccc gctgaaccga tagcacgttg tgccatggct gtcgccaggg cagtaagacc
    35881 gattgccaaa gcggctgcca aagcaatgat tcctttttcc attcattctc ctttcgtttt
    35941 ctatccaatt caatgatcta aaatacgttt cttaaagggc gtaaagcctt gctctccacc
    36001 ttcatagaaa cgccggaaga attcaacata ctgcaaacgc aagctctgga tggtcgggct
    36061 gaatgctgcc ataataatat tgagcgaatg gatcagaatc gaaacaagga ctcctgcgat
    36121 tacatttccc atcataccta ccatatcatt cgcaactttt gccagaaaaa ccgacgccag
    36181 tcccagagca gcgatacgca agtacgaaag gatatttccc aacaacccaa tgaactcaat
    36241 aggacccagg atcatgccgg tctttccata cgatattccc agaaagatga caccggctgc
    36301 cagcagagca tatccacccc atcttccagc ggctggaatt acttttaatg ttgcgccaac
    36361 tacgaccaat aatcccatga tcccgatgaa cattccccct ttttctaaca aatgcgactt
    36421 gttcttatgt ttgagggcat ttgcaattcc caggatcaat ccgagcagga tatgcaccac
    36481 acccacacca atggtcatat acagcagagt gatcgcacta cccgatgcag tgcggtcaag
    36541 taaaacgggt tttagcccaa tgctgcttcc catagttccc ataaactcac caaagagaaa
    36601 accaaaaccg atcgcccaga gagaaccgat tttgatggtt ttgatcaaat cggcaaagat
    36661 acccgtttta atcttctttg ccaggaacaa ggtcagaaaa aacaggattg cgccatacgc
    36721 cacatcgcta accatcattc cgaaaaagag gggcataaaa atagccatta aggtactggg
    36781 atcgatatcg gaataggcag ggatcgcgcg catttttgtg agattctcaa agggtttaat
    36841 gctttgggag ttttccatgg caacaggaat ctttttatgc atttcttttg gaatttccag
    36901 ttgattaatg accaggtctt ccccaatttt ttcttgaagc cggcttttca gatcctgaag
    36961 ttcatcagca ggaacccatc catacgctgt aaatgtaaat tccgtttcgc cgaggcgtga
    37021 gtagatctca aattcatcca gcagattcag acaagtgacc tgccagtttc gcaggaatgg
    37081 tccccaggca ttggcgagct cgcgaaaatg gccctccagg gcatccagct ctctatgatc
    37141 ttccgcgatc ctctttgtag tcatttccaa ggcttccgaa gcggaaagaa gtgaatattc
    37201 ctcaggtaga acaagttggg tgatcttttc tttcgtaaga agttcttcta ctgaaacaac
    37261 aaattcgcgc ggcaaaacgc cgattagggc attcgttgac ccgttgacct tgacgatcgc
    37321 catctcaaat tttccctggg tcagagtgcg caattgattt accaacatgt tcatggaatg
    37381 ggtttgggta gcatgcagca gagcccccac ggtggcattt ccgggttttc gagcggattc
    37441 ggggagtaga ggagccatca actttaatat ttcttgatat tttgaaaggg taatcaggtc
    37501 ttcttctaaa gcggtgcgat tttgagtgaa ctgctgtacg atcgtagtca cctggtctac
    37561 tttttccccg atctcttcag ttgtagaatt atcttccaaa ttctccactg aaccgaccag
    37621 atattttttg aacagatctg cgaacccgtt gattctggcg ttgatcaact cgaccttttc
    37681 acgcttttgg accatttcat aggtcaaatt taatggctgt aaaagaaggt tcgagagaga
    37741 acgaatatca tcgatctgca tggcacccag gttgtgcagt tcgtgaatgg tattttgaag
    37801 gtcctttttt aatccaacga tctcaatcct tgccattttg cgaatcatag gaaatctcct
    37861 tcgatccact acccaggata aaatcaagtc caagggctac tgcctggggg atgaattttt
    37921 ttccattttc aatgattttc gaagtttttg attcagataa atgaagtttt tcttctgcag
    37981 cagcatttgc cctcaaaata ccgttttcga tcagccgcgc tctggctcta cgtccgttcg
    38041 cataggcttc ttttttagaa ttttcagcat cttcttgaac tttcgcgatc tgccggtcag
    38101 catctttttt tacttcggaa atgcgttcag cagagcggat ctcagcttgc atgatcttct
    38161 ccaataagat ggctggatct tcctgtatat tttgtatgct cgctgcagtc cttttcatat
    38221 tttgattata aacctcgcaa aaaatggaca cgctatcgta aacccacctg aatttttcgt
    38281 caacctcaag aagtcacata atttcaagtc ctggaagagg gaaaacaagt ataatcgcta
    38341 aggaatttca ggttgattag agcattattt gaagatggag cgcaaatagt atgaaagaat
    38401 atgatgtcat catcatcgga gctggtcccg gcgggtatgt ggctgccatt cgaacatcac
    38461 aacttggatt gagaaccgcc atgatagaaa aaagatggtt ggggggagtt tgcttgaacg
    38521 ttggctgtat cccttccaaa gcactcttaa agaacgctga aatcgcccga acgctcagag
    38581 aagaagccaa agaatacgga attcagggtg agaacatcac cgttgattac tctgcagccg
    38641 taaaacgcag ccgacaggtt tctgaccggc tgacgcgcgg tgtgagtttt ttgatgaaaa
    38701 agaacaagat cgatgtttat gaaggcagcg ctttcctgaa agccaaagat cttgttgccg
    38761 ttacgctcaa ggatggaaaa agcgaagagc tgaaagcggg gaatatcatc cttgccaccg
    38821 gcgcacatcc tttcatggct cccgagtgga accacgatgg caaaaaggta ctgacctatg
    38881 aagatgccat tttacaggaa aaacttccaa cctctgtgat catcattggc gcaggcgcca
    38941 tcggggctga gttcgccacc atctggagct ctttcggcac aaaggtcagc cttgtcgaga
    39001 tgctgccgaa catcctgcct ctggcagatg aggaattgag caaagagcta caaaaggcat
    39061 tccagaaacg cgggattgaa attcatacgg gtcatcgagt ggagaccatt gagcccacca
    39121 gcaatggcgt aaaagtaacg ctatcaaccg gcaatgaaaa gaagttgttg gaagccgaac
    39181 aggcgctggt ggctatcggt ttcaaagcca acaccgacga acttggctta gaagccgtgg
    39241 gggtgaccct ggataaacgc ggtttcatca atgtggatgc caacatggca accagtattc
    39301 ccggtatttg ggcaatcggc gacgtcaccg gaaaattgct tttagcgcat gcggcttcag
    39361 ccatgggaat tcactgcgct gaaaccatta agggcatcaa gagaaaaccc atcgatttta
    39421 aaatgatccc gagcgccacc tatagccacc cgcaagttgc ttcatttgga cttaccgaaa
    39481 aggaagccat cgaagcgggt aaacagatca aaaaggggaa attccccttc caggcgaacg
    39541 gcaaagcgct tggacttggc gaaagttccg gtttcgtaaa agtgatcgtt gacgctaact
    39601 atggggagat ccttggtgct cacctgatcg gtcccgaagt ttcggagttg ttgcccgaat
    39661 tgagcctggc acagcaatcg gagctgacag tagaggaact tgcgcataac atccacgccc
    39721 atcccacact cagcgaagcg atcatggaag ccgctgaaga tgccttgggg cgcgccattc
    39781 atatttagaa acagcattgt ttttcaaacg tttaaacaat gggatggaag gtgaaaagcc
    39841 aacccattgt ttattttttt aacaccccta tgctataatc gccttcgcaa aggaaaacaa
    39901 tgttaaaaaa gaaatttaga gcatctgata agacatcagt aagcaaagat ttcaaaaagt
    39961 tcactcccct tctcaacgag atcaacgacc ttgaaaagac cttcgagcca ttatctgatc
    40021 aggaactggc tcaaaaaacc attctgttta agcaacaatt ggaaaatggg aaaactctct
    40081 ctgatattct gccccaggca ttcgcagcgg ttcgcgaagc cagcaagcgc acccttggat
    40141 tacgacatta cgatgtccaa ttgatcggtg gcatcatcat gcacgagggc ggcatcgcag
    40201 aaatgcgcac cggggaagga aagaccctgg tcgccacttt acctctctac ctgaacgcgt
    40261 tgagcggaaa aggggctcac ctggtgaccg tgaacgatta cctggcgcgg cgtgacgccc
    40321 gctggatggc gcccatctat tccgcactgg ggttgagcgt gggtgtctta cagatggcaa
    40381 accgaactga gaacggtcac atggcttttt tggttgacct cgaaaaacac gcaagcaaag
    40441 aagaggatga ccaattgcaa atagtccccc gacgcgaagc gtatcaagcc gatatcactt
    40501 acggcacgaa caacgagttc ggcttcgatt acctgcgcga caatctggta agccgcatcg
    40561 aagatcgggt acagcgcgct caccattatg ccattgtgga tgagatcgat aacatcctca
    40621 tcgatgaagc cagaacgccc ctcatcattt ccggctctgc atcagaagac attgcctggt
    40681 acgaaaaaat ggcgcttatc gtccgccagc ttgatgaaga agattatgag ttcaatgaaa
    40741 aagatcgcag tgtttccctg acagaagttg gtgaagtgca cgccgaggag ttgctcaaca
    40801 caaccctgcg cgatccggat cgcccggaag atataacccc cgaacaagcc aggttgttgg
    40861 gctatctgga acaggctctc aaagcccgtc tgcttttcac gaaaaataaa gattacatca
    40921 tccaaaacgg cgaggtcatc attgtggatg agttcaccgg tcgtttgatg cccggtcggc
    40981 gctggtctga agggctgcac caggcaattg aagccaagga aggtgttagg gttcatgcag
    41041 aaaatatcac ccaggctacc atcaccctgc aaaattattt tcgcatgtac gaaaaattgg
    41101 ctggcatgac cggcactgcc tacaccgaag cggaagagtt ttacgagatc tataaactgg
    41161 atgtggttcc catccccacc aatctggatt attcagcagg ttcacccacc tcagatctga
    41221 atagctacca gaaaaaagat gatgagggat atgaatacac atactatgcc gcaaaaagcg
    41281 acccggaaca aaaagaactc ttttggaaga gaaaagacta ccccgatatt gtctatcagt
    41341 ctcgtgaagc caagttgcgc gcaatttgca ttgagatcac acgtttcaac gctattgggc
    41401 gccctcagtt ggtaggcacc acctctatca tccattccga agagctttca gcccgcctca
    41461 agcgcgagtc tttgcgctta cttatgcaaa tcctggtttt gaaatcatta tggcgggaaa
    41521 agtttgggat caacgacgct gaaagagcga ttcccg
//
LOCUS       DAOSDS010000009        40984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_536_length_40984_cov_4.938552, whole genome shotgun sequence.
ACCESSION   DAOSDS010000009 DAOSDS010000000
VERSION     DAOSDS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 40984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40984
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_536_length_40984_cov_4.938552"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="PLL88_02165"
     CDS             <1..877
                     /locus_tag="PLL88_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ00398.1"
                     /translation="AKLDNLAGHLFIAPQFLGFLLFVAIPILAVIIFSFQDRNLLTGK
                     WDWIGFDNYSFMFTKDPYFYKVIGNSLIFSFGLIIMNVSLALFLALILFQNKRGMIFF
                     RTLFFSPVVTSTVAWVLVWSFMLQSNQGTINQFLKTIGIQGPNWLGSTQWAMFSVIFI
                     RVIKNVGLNMVILIAALKDVPHELIEAARVDGANMFQCVRKIIIPLIAPTILLVIIIT
                     LIGSMNVFDHIMLLTAGGPSNSTLVLAYYVYYQAFLTNEIGYASALAVVLFLVVLGLT
                     FFQWNMRKKWVSYEQ"
     gene            867..1730
                     /locus_tag="PLL88_02170"
     CDS             867..1730
                     /locus_tag="PLL88_02170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ00399.1"
                     /translation="MNNKQYLPPLIPKGFRNFLFYFGLLLLAVPFIFPFWWMATSSLK
                     TYKEIFAYPPTLLPGSWHFENFITAFTYQPFARQYLNSLYISLVVTAGVLVISSLSGY
                     AFARINFKGKNFLFMLLLSSLMMPPEVTIIPNYLIMNRLGWTNTHIPLIIIPILGAGG
                     VFATFMMRQFFISLPKDMEDAAKIDGLGWWGIFWKIGLPLAKAPLSAVAIITFLNSWN
                     SFLEPLIYLNDTKKYTIPLALTGYIDAYGAPMWSVQMAATTLSVLPVLIFFILAQQQV
                     VESFAMSSVKG"
     gene            1752..2750
                     /locus_tag="PLL88_02175"
     CDS             1752..2750
                     /locus_tag="PLL88_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ00400.1"
                     /translation="MRSEKIYSIGVIGLGVFGARHASICLNHPNFRLVGVADVKGELV
                     ESYSKEFGVPGFTDYRGLLAVPEMDAVTIAVSDQFHHDPCKDSAQKGIHIFLEKPIAD
                     TSTHAKEILDAVREHDVKLMVGHTLRFDPRYLAIKRVVDEGKLGEIVHVYARRNATVK
                     SGRRLQGRVDAMIFQGVHDIDFLQWLTGSKVSKVYAEATSNILKDLKVNDTTMATLRF
                     ENGVIGLLEQSWALPADMPSPLDVELEVVGTEGSAYLDLRSQSISIVSDGKLNQPDLI
                     YGLPDQHFLFDEYNHLAKYLSGEIEARVSGEEALSALEVAEAIMHSAKSGNPILLA"
     gene            2768..3475
                     /locus_tag="PLL88_02180"
     CDS             2768..3475
                     /locus_tag="PLL88_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00401.1"
                     /translation="MMSRFVNELMKARKEKKLVLIASLPRNDAELARAVLDAGADAVK
                     IHMNVHHHASNTTFGSLDEERENLTAILKVSEGKLTGIMPFAAPVNAPDTLKELSDMG
                     FDFYSQYLSHAVSGCFPSSEKPARMLALAVDDPIELASGLDRMPMEVCELSIMDGNTY
                     GQPFTYHDLLRYSAVRVRTGKPLVVPSQHLITPESVPDLIEIGMDGLMIGAVVAGSTA
                     ESWGKSVRAFRKMIDKK"
     gene            complement(3521..4717)
                     /locus_tag="PLL88_02185"
     CDS             complement(3521..4717)
                     /locus_tag="PLL88_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOJ00402.1"
                     /translation="MEKATRQHTKEHNRALVLRTIFAHEQISRAEIARVTKLTRTTVS
                     SIVSDIITEGLVEEVGVGTSYGGKNPILLSLVEDSRWMIGLDLSHNEFRGAIVNLRGV
                     ICDVIAMSIEGYDGDDALEILYQIVDRLIKKAATHPLLGIGVGTPGLVNTQEGVIINA
                     VNLNWKDLPLTHLLQERYNLPVYVLNDSQAAAIGEKTFGKGYSPEESMVLINIRHGIG
                     SGIIIHGSLFQGDGGCAGEIGHIVVVKEGGELCRCGNHGCLETVASAQALIKKARAGI
                     KDFPESILPHEPKQINLAAIQKAFNANDPFAQRLVFDTAHYIGMVISDVVSILNIQKI
                     VFVGDMTCFGEPWLNAIRNSAQKYSLQKPILNTKIEIGQSGENSIILGATVILATDYS
                     LLFTHS"
     gene            4860..6467
                     /locus_tag="PLL88_02190"
     CDS             4860..6467
                     /locus_tag="PLL88_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HOJ00403.1"
                     /translation="MKDFLWWRDGVIYQIYPRSFLDTNADGIGDLPGIIQKLDYLKEL
                     GVDALWLSPIYPSPDVDYGYDVSDYKAIAPQYGTMQDFETLLTQAHKRNVRIILDLVL
                     NHTSDQHPWFIESKSSRDNRYRDYYIWKDPKKNGAPPNNWLSVFGGSGWEFDAHTGQY
                     YFHHFFKEQPDLNWRNPTVRKELIDVFRFWLDKGVDGFRLDVFSAYFKDADFRDNPKK
                     LIGLRKYDRMIHLYDADRPEMLPLLQEIRALLNRYPERYAVGETFIATFSKAAGYCGD
                     NLLHAAFSFDKFMNGPWKAKRFLRAIQKWESALEGKCWPNYVLNNHDVKRSATRFGQG
                     EDDERLKVAIALLLTLRGTPFLYYGEEIGMRESRFKKSEILDPVGKFYYPFYKGRDGC
                     RTPMQWDASEHAGFSTVKPWLPVHPDYLERNVSAQQKDDRSLLAFYKKLMNLRKRHAA
                     LMQGDFSLVPHCPANTLVYFRSAAEERLMIALNFDGRSKKIALPAQDANQYALLLSSM
                     EGKPQQIAGDDYLLSGNEAAIFAIKRS"
     gene            6571..8223
                     /locus_tag="PLL88_02195"
     CDS             6571..8223
                     /locus_tag="PLL88_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOJ00404.1"
                     /translation="MTRIREERKRWEQETFRPVIEKYPERQPEFSTPSGIPVPPAAIP
                     ADYDYMEKLGFPGEYPFTRGVQPSMYRGRFWTMRQYAGYATAQESNERYRYLLQQGQT
                     GLSVAFDLPTQIGYDSDESIAAGEVGKVGVAISSLEDMELLFREIPLDKVSTSMTINA
                     PAAVLLAMYVAVAKKQGVEPGKLRGTVQNDILKEYVARGTYIFPPKPSMRLITDTFQY
                     CQQNIPRWNTISISGYHIREAGSTAVQEVAFTLSNAIAYVETAVKAGLQVDDFAPQLS
                     FFFACHNDFLEEIAKFRAARRLWATIMKERFGSQNTNSMRLRFHTQTGGSTLTAQQPE
                     NNIVRTTIQALAAVLGGTQSLHTNSMDEALWLPTEKSVRIALRTQQIIAHESGVADSV
                     DPLAGSYLVEYLTDEIETRAKQYIRRIDDMGGALAAIENGFIQREIQDSAYTYQKSVE
                     EHHHVLVGVNDYQIAETIDIEALAVDPALEQGQVERLKKFKENRDTGKVHELLSVLEQ
                     TAKTDQNLLPLMIHCLEQKLTLGEICGVLRTVWGEYQPPVWA"
     gene            8245..8646
                     /gene="mce"
                     /locus_tag="PLL88_02200"
     CDS             8245..8646
                     /gene="mce"
                     /locus_tag="PLL88_02200"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOJ00405.1"
                     /translation="MEIKKINHVAVAVEDITRATEFWQSVLGLKLDHVEEVESQKAKV
                     AFFPCGESEVELVEPTDPESGTARFIQTRGAGIHHLCLEVDDIDKALEELKAKDVRLI
                     DETAVVLEGRKMAFIHPKSSGGVLIELYQII"
     gene            complement(8686..9993)
                     /locus_tag="PLL88_02205"
     CDS             complement(8686..9993)
                     /locus_tag="PLL88_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00406.1"
                     /translation="MEDKKKKKAIWAWTMYDWGNSAFATTIMAAVLPVYYSSVAGATL
                     APNIATARWGFTTSFAALLVALLGPILGAIADFKGNKKRFLSIFLGLGVTATALLYFV
                     RTGDWLLASILYIFGNMGFSGSLVFYDALLPHVASSDEIDQVSSKGFAMGYIGGGLLL
                     AINVAMIMFGETLFPGIDPTLMSRLSFLSVAVWWFIFSLPLFRHISEPPRRIQKDEEG
                     LNPLKVSLQRLGKTFKEIKNYRDLFTFLVAFFLYANGIGTIITMSTIYGAEIGIGQTT
                     LIGTLLMVQFVAAPFAIAFGALAKKIGAKKSIYISLVIYTMIAIAGYFMSVPWHFWAL
                     GFGVAMVQGGSQALSRSLIGRMMPKSKSAEFYGFFSVFEKFSSIAGPLLFGLVSTLMG
                     ESRLSIVSLIIFFILGMIVLTRVDVERGIQTAREEDESMITVK"
     gene            complement(10180..10599)
                     /locus_tag="PLL88_02210"
     CDS             complement(10180..10599)
                     /locus_tag="PLL88_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00407.1"
                     /translation="MEKQASSGKRFGYIIAMLVNAALIYVFNNILSWNIPFLLPTFED
                     CLWVIRLSLSATIFVNFIFIFFDVNWFHHLMQVILNVFSWLSTYFLYTIFPFEFPSEL
                     WIQIAKIVFIFLLVVIPIGTLVELIQFLRKVNRRQDQ"
     gene            10781..11233
                     /locus_tag="PLL88_02215"
     CDS             10781..11233
                     /locus_tag="PLL88_02215"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOJ00408.1"
                     /translation="MADDTLLQKTVESFWDTIPPVWGRVRENARGNAIQDFNLTLIQF
                     HILRHIWHGIRSVRELAEKQQISRPAISQVVEVLVLRGLVVRKQDTRDRRCVQLELTA
                     TATKLLEAVFSKNRRWMAEKMVSLEQSELETIMAAMEILKKTFDPSVR"
     gene            11243..13006
                     /locus_tag="PLL88_02220"
     CDS             11243..13006
                     /locus_tag="PLL88_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00409.1"
                     /translation="MATISKILKFVKPYWKLALLSMLMLLLMVLSDLAIPRLIATIID
                     DGIRQNRMDVMWHTSLMMVGIAVINTVAAVLNNIFSVRVGEKVARDMREALFLNIQQF
                     SYGDLDRFSTGKLMVRLTSDIAAVQLMVRVSLRIGARAPLLMIGSIILMFLTSARLAT
                     AIIPILIITSAIIILFSIKMEPLFRAVQQKLDHLNTVLQENIAGARLVKAFVRADHES
                     QRFGEVNEDLTLGTVRVMQFMSSMSPALTIFVNIGMILVIWYGGLQTIQGELQIGQVV
                     AFTNYLLTTMTPLILMANLSNTWANGLASAKRINQILDVEPEITFPTQEAQIPQGWSN
                     AVEFSEVSFHYNGNNNLEVLEGINLRARPAQIVAVLGATGSGKSSLVNLIPRFYDPSR
                     GEVRIGDLNIKVLKEATLLSQIGIVPQESILFSGTVGENISYGKACATDEEIEMAAKV
                     AQAHDFIMSFPKGYASYIEERGANLSGGQKQRIAIARAIITRPRILILDDATSSVDVE
                     TETKIQDGLREYVQGSTIFMVAQRISTVLNADRIIVLDKGRLVAQGSHAALMKKSRIY
                     REIYESQLGNGLASYEKEALQ"
     gene            13003..14814
                     /locus_tag="PLL88_02225"
     CDS             13003..14814
                     /locus_tag="PLL88_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00410.1"
                     /translation="MNRPTDVRSSMQRRPIHSVPIEKAKDPSRSIARLLPYLKSYKRQ
                     IGWVIVFIVLYTLLGLIGPYLMGVAIDIFIAEKDVAGLLRTALWMLLVYLLSNGLNVW
                     ANWIMARVSQFALRDLRRDLFDHLQAQSMSFFDTHSAGGLMSRLTNDIDAINQAISQN
                     ITSLIASTLSMGGIVVAMFVLDPWLALGSLLVIPIMAWFTRFVAKYTRRGFRELQKDL
                     GELNAIAEESISGQKVVKAFQRNENVVAAFRKQNEAVYAAGVYANSYALLLMPLTQVL
                     GNIFVIVLATLGGWLALQGLASVGMIATFINYGQNFVSPLRQIANLYNSIQAALAGAE
                     RVFEVIDNPPEIQDAPAAISLNEIRGDVLFKNVDFSYIPGRPIIKNTTIEAKAGQTIA
                     LVGPTGAGKTTIINLLNRFYEIQGGSISIDGHDIRSVKKADLRKSLGIVLQDTFLFSD
                     TIMENIRYGRLDATDEECIQAAEMADADHFIRQLPHGYQTNLSERAGNLSQGQRQLIA
                     IARAILADPAILILDEATSSVDTRTEARIQKSLLRLMKGRTSFVIAHRLSTIRDADKV
                     VVINQGEVVEQGTHQELLDRRGFYHHLYISQFKGKAI"
     gene            complement(14885..16126)
                     /locus_tag="PLL88_02230"
     CDS             complement(14885..16126)
                     /locus_tag="PLL88_02230"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00411.1"
                     /translation="MLTTIKKTYHEFPKTFWNVMLGMFVDNIGSSLLFPFLALYVTQK
                     FGVSLIDIGWIQVVSTMVNVVGTSIGGALADQFGRKTIGLIGLFGSALITLPLGLTNR
                     WDLFIVFRLLSSFMGNFANPAFQALMADILPSEKRSQGYAMLRIIGNLSAAIGPFLAG
                     FLVGHSFLPLFIMDVVLSVATGIFLLVIMPETKPEEPEHSAKQSLKTTFAGYGMVLRD
                     RVFVTLVLLIVLTMMVYMNYNTTLSVYLNRLHGISAKQFGFLLSLNAMMVVAFQFSIS
                     RITARYKQPLMMFLGTLLYAIGFSMFGSGNSYAYFVVAMIIITIGEMISAPLYQVIAA
                     EFAPIDMRGRYMAFLNISYGFSAAFGPLAAGYGLESSHPQWVWYGGGILCLIAALGFL
                     NTRMKPAENVAPTPDAGSDSL"
     gene            16412..16987
                     /gene="rplI"
                     /locus_tag="PLL88_02235"
     CDS             16412..16987
                     /gene="rplI"
                     /locus_tag="PLL88_02235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOJ00412.1"
                     /translation="MKVLLIRDVYNLGRAGEVKKVANGYGRNFLIPQKLAVMVTPGAL
                     KQVEKIQSAAEKRRVALNEEMAGVAEKIQGLELSFTAKVGETGHLYGSITQQMIADAI
                     EEKTSIKIDRHWIESQPLREVGLHNVKVRLTFDLIPEVAVTVLSEEEVEKALKEKAKE
                     ERKARKEKKEEKLPTIDEIPSFTEPNQDDVK"
     gene            17060..18604
                     /locus_tag="PLL88_02240"
     CDS             17060..18604
                     /locus_tag="PLL88_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOJ00413.1"
                     /translation="MSKTVGQILKETRALKDISLQEAARATHIRLQYLQELENDRPEL
                     LPSRAQARGFLRLYAAFLELDGTQLLTLLESPPLPDEPLEDIPKPEESPELKSEAEEK
                     KEESVAEPALQKPSAQPKKTNKVSAKLQALISKLPKFGKKKTTSPTPSSQAEDQAPTQ
                     LPPASKKGSKKEDRSAGKRKQASKNPARRVSPPAVPSEDTFKEVGSQMRRRREKLGLS
                     LSDVEQFTRLRRIYVQAIEDGKFEDLPSSVQGRGMVANYAHFLEMDQDAVLNLYAEGL
                     EAQRREKLQEKWTTKEPGIRLSIQLPEKIRRFLSPDLLLGSLVVVALFVFIFWGASQI
                     FGDKSETAPTTAPSISEMLQSTTTPTLALESTAGLTGTESDEPTALAMETQVATQPVA
                     PIATANAAPLQLYIVAQQRAWMRVTVDGNVEFEGRIIPGNAYTFSAQERILLLTGNGA
                     ALEAYFNQEYLGALGKIGEMVNLDFSAAGLSVPTPTITPTVLPSATPTPAPTFTPTPQ
                     GTATEE"
     gene            complement(18611..18802)
                     /locus_tag="PLL88_02245"
     CDS             complement(18611..18802)
                     /locus_tag="PLL88_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ00414.1"
                     /translation="MLTKRQIQCLRLAANGLTARSIAQELGVTERMVRTHLREARTRL
                     EAHSTAEAVYHALKQELLE"
     gene            18999..20321
                     /gene="rimO"
                     /locus_tag="PLL88_02250"
     CDS             18999..20321
                     /gene="rimO"
                     /locus_tag="PLL88_02250"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOJ00415.1"
                     /translation="MKRSFYIHSLGCPKNSVDSQSMAELLIKQGYSVANSQQEAGIII
                     VNTCGFIQPARQEALNLLKEIMRQKKASQKVIAAGCLTQREAEMVKREVPGLNALIGT
                     RRWMDILAVVRQLDMATAQPLTRIADGKLEMEEEHIIRASNLGGYAYLKIADGCDRGC
                     SFCAIPLIKGPQVSRDIADILRDVHFLQEKGVQELILIAQDVTAYGRDVGMSGSLPAL
                     LEQIVKIATAVPWIRLLYTYPGEISQHLIALMAANPQILPYLDIPLQHASPRILKRMH
                     RPTGVEKIIAFIQDIREQIPHLAIRSTFIVGFPGETEQDFELLLDFIRSMRFDRVGIF
                     PYSHEPGTVAFQYADDISPQEKEERVQRLAELQEEISEQKNREYVGKELDVIVDGVGD
                     GISVCRSYRDAPEVDGLVMVQGELEIGKITAVEVTAALVHDLVAVRSR"
     gene            complement(20331..21392)
                     /locus_tag="PLL88_02255"
     CDS             complement(20331..21392)
                     /locus_tag="PLL88_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00416.1"
                     /translation="MAKNNAEKTAEIKVDQKTTKEDTQAIKTGGKRKGGRPRYVRAVL
                     MGLLLVLIAALISGSLGYYSGIKQRKAEEEKQRLTLATTHYKYGMEAMASGNYEVARI
                     QFEYVIQIYPTFPDITEKYSEVLINLTKTQQTTAQATATPTRDIQGAQALFQQAQTDM
                     ANQEWCLAVDTLKSLRDEDVSYETLTVDGMIWASLRNCATYKITNEGDLEGGLYYLSL
                     AQKFAPLDHDAVNYAAWARLYVTGASYWEVDWAQVVYYFSQTYAAFPYLHDTSGWTAI
                     DRYRIGLREYGKYFMSIEEYCSAAEQLQLSLNIQYDEEVSNLLNEATVYCQGPTAEPQ
                     AITTITTEATSEATAEPTE"
     gene            complement(21430..22998)
                     /gene="murJ"
                     /locus_tag="PLL88_02260"
     CDS             complement(21430..22998)
                     /gene="murJ"
                     /locus_tag="PLL88_02260"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOJ00417.1"
                     /translation="MSKLTRATIIIALSFAVNKVLAIVRQLIIARQFGLSAELDVFNV
                     ANNVPDMLYSLISGGALAMAIIPVLSEVLTKEGRSDGWRVFSQMANFAFLVTAALSLV
                     VGVFAEPLVKSPIGIAPGFSPEQQALVVSIMRLDLIATLIFSMAGLLVAGLQANQHFF
                     LPAVAPIMYNLGQIFGALILAPVEGLTIAGITLPAYGMGVYGIVYGVLIGSLLYLIVL
                     IPGLFRFQFKWFPGLGKRDPYVKRILAMLAPRVGSMLCYQLTFIARDNIASYLSEGAP
                     TALTYGWMIQQVPETLIGTALGTALLPTLSELFARKDQAGYHATIEKVERVIIALAIP
                     ITVVLSLVLRPFIETAFGFDTAATDQLLWVTRGFLVGLLGHCLLELGSRIFFSQQNAV
                     IPFLASAINFGIYILAGTLLSSRLDATGIALADSIAFSIQAAFLLILFVLRDRKSTRP
                     EKGEEEKKTTRELGQTIWRTIAGSATAGAIAWLVMQRSAGLPLLVQGMASFLFGIGAV
                     LPFIWKELKVFLNL"
     gene            complement(23021..23833)
                     /locus_tag="PLL88_02265"
     CDS             complement(23021..23833)
                     /locus_tag="PLL88_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOJ00418.1"
                     /translation="MIAFLNQFNPVIQALFGTLFTWGVTALGAAGVFLSKEVNKKLLD
                     AMLGFAAGVMIAASFWSLLAPAIEIAGSVSSKPWLAPAIGFLMGGLVLWIIDKILPHL
                     HMDMPVQMAEGIKTTWQRSVLLVLAITLHNFPEGLAVGVAFGAAAAGVEGATLSGAIV
                     LAIGIGIQNFPEGLAVSVPLRRDGLSRWKSFWYGQLSGIVEPLGGILGAAAVVTMRHI
                     LPYALAFAAGAMIYVVIEELIPEAQHGENTDIATWGALIGFTVMMILDVALG"
     gene            23995..24663
                     /gene="rsmI"
                     /locus_tag="PLL88_02270"
     CDS             23995..24663
                     /gene="rsmI"
                     /locus_tag="PLL88_02270"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOJ00419.1"
                     /translation="MRSGILYMVATPIGNLQDITVRALDVLQSVNRVICEERRVGSAI
                     LHQLKVEGKTLVELNEHNEEQQAQELALALLNGENMALISDCGTPAFADPGSLLVQLA
                     YDYGIRVVPVPGASSLMALLSVSPLPLKKFYFAGFLPRKEEERKKSLEQLRRLKTPLV
                     LMDTPYRLERTLQDIQNAFGKGRMITIGLNLTLPTEKVMHQAVGEVLKKLKGQKAEFI
                     VIVH"
     gene            24961..25164
                     /locus_tag="PLL88_02275"
     CDS             24961..25164
                     /locus_tag="PLL88_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00420.1"
                     /translation="MDSRRPGCLSGIMKLFFLDRIYDWSQRRFGYKSGGCMGCGCGTI
                     LFIIFVYVLIRIIFGTDWLRIGF"
     gene            25181..25918
                     /locus_tag="PLL88_02280"
     CDS             25181..25918
                     /locus_tag="PLL88_02280"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOJ00421.1"
                     /translation="MRKTLKVLMGIFALSILVACGLPLRNTNIQGSGRVVTDDRTVSN
                     FNSISLEGIGDLEIVQGDVESLTIEAEDNIIGKIESYVSGSTLHISFERFINIIPTSG
                     ITYTLTVKDLSRVEVSGYGDVYMASLTTDRLEVEVSGGGRVKIENLTAASLDVELSGA
                     GDFDIAGVVEEQEITLSGAGSYKAADLQSKIVEIDISGAGSAQVWVTDELDVNLSGVG
                     SLQYYGDAVVRQNISGIGKVVSLGAHE"
     gene            complement(25968..26807)
                     /locus_tag="PLL88_02285"
     CDS             complement(25968..26807)
                     /locus_tag="PLL88_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOJ00422.1"
                     /translation="MLKIVMDTAGDLCTGWQEKYQINLLPINIIHNGVSYQQGIDIRY
                     DDFYAMVEQDNSIPSTSQPTPYQFVEFYKKIAQPGDSILSIHVTDKLSGTMNSARLAA
                     EELKGSYHIIPFDSASGTICMGMMCKEARQMDAAGKSVNEILEKLESIRRKMELIFTV
                     DTMKFAKLSGRVKSLQATLASILDIKPIIELRDGTIEIKDKVRSRKASLERILTRLKE
                     KIGDQRAYIAVVHAHDPITGKAVVEEVKKRFNYVEVAFAEISISLAVHFGPGTIGLAA
                     YPE"
     gene            complement(27001..27723)
                     /locus_tag="PLL88_02290"
     CDS             complement(27001..27723)
                     /locus_tag="PLL88_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOJ00423.1"
                     /translation="MTALLYQLDSYQKEFDAQITAIHEELHAIQLDQTCFYPGGGGQP
                     WDNGSLTSDNDDLAFTRARMVNGEHWHLLDAGTKLPAIGSPVHGTIDWEKRYRLMRTH
                     TAMHILCGVIFRDYGALVTGGDMQPLEARMDFEFESMTKELVAVIEEAVNREAQLAHP
                     IRVKILPRAEAFQIPDLIRTKINLLPEHISEIRTVEIVGLDLQADGGTHVANTNEVGK
                     IRISDYMSKGKINKRIYVKIEE"
     gene            complement(27724..28938)
                     /locus_tag="PLL88_02295"
     CDS             complement(27724..28938)
                     /locus_tag="PLL88_02295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00424.1"
                     /translation="MQKIDIIKTRTAIRNHPLWQTAILSSAHMLNDFFGGMLSPLLPI
                     LILNFGLTKFEAGLLNACLQWPSVSQPFFGRLADRVNLQKYIFLLPLTTGVFMSLVGS
                     APSTAILALSLFISGLSSSIFHAVASPIAGKVSGKHTGRGLSLWMACGEAGWMLSPIL
                     IIAYINKFSLNRIFYLIIPTFIVSVLLYFRLKKIEAIKTHVKVSNGSAKSSLKAITPM
                     LLPIIAIVCARSLIQATTTVYIPTYLTEMGVNLWLAGAALSLTTGAGVLGAIMGGIYK
                     DKIGGRPVLFASLIGSSVFFFLFLYSAHQVWQIVTLFLTGLFSGMYLPVALAMVQEYS
                     PENRSFSTGIYQAFLFTIGAIASMIIGYLYDVLGAHPAFIISGSIGLVGTVLVFFIPS
                     SNTAHQETAQEV"
     gene            29425..30714
                     /locus_tag="PLL88_02300"
     CDS             29425..30714
                     /locus_tag="PLL88_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00425.1"
                     /translation="MLKNITNLYRKFNKDFWILVGASFIDSVGGALIFPFLTLYMTQK
                     FNVGMTEVGTMFLIWGLGAGLLGNSLGGAIADKFGRKKSIIFGLIASASSALAMIFIK
                     DIVVFYIVIGVIGIFEDIAGPARQAMIADLVPEELRTDAYGIMRIEFNLAVTIGPAIG
                     GIMAARSFSILFIMDVFISLAVAGIVLLFLPETKPQMQEHQREESLKETFQGYRKVFK
                     DKLFIAFVVVMMLETLMYFNMNSTLSVYLVNFQGITTEQFGYVLSLNAVMVVVLQVFF
                     SRWVANWKPMVTMALGNILYVIGFSMYGYVNSYPLFLLAMVIITIGEMIIAPVAQSLV
                     AHFAPEDMRGRYMAVHSLAWILPVSVGPLGAGYIMDNFDPRLLWFVAGGIGLLDVLGF
                     LLLHFKAGKKFEERQNGYKDKSEDPSVQVETTPMIAD"
     gene            complement(31021..31284)
                     /locus_tag="PLL88_02305"
     CDS             complement(31021..31284)
                     /locus_tag="PLL88_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOJ00426.1"
                     /translation="MKIEASIINELGIHAHAAIQFVQTACKYKATVRVMHNNKEADGK
                     NLIRLLGLRIEKDAHITIFAEGEDEQSALQDLKLLIENNFGEE"
     gene            complement(31394..32383)
                     /gene="tyrS"
                     /locus_tag="PLL88_02310"
     CDS             complement(31394..32383)
                     /gene="tyrS"
                     /locus_tag="PLL88_02310"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOJ00427.1"
                     /translation="MGNLSLQSQLAAFLDGSEFGDKNLEARMRHELEERLQQTALEKR
                     PLRVYCGYDVSGPDLHLGHTVTLRKLHQFQDRGHDVTLVVGTFTSLIGDPSERETARQ
                     PRSVALIMEESKRYAQQAFKILDAQKTRLRFNHEWLNDLQVSDFIPIAAQFSFQQLLS
                     RENFRKRIEKGDTVRFQEILYPVVQAYDAVELRADVQLGATEQLFNLMTGRKLQEYFG
                     LPPQICLTFPILVGIDGEKRMSKSTGNYIGINETPIEKYRKLFQLPPKALPSYIRLLT
                     RWSPDEKNKMISAFQKQTRDPNEIRRKVACEIIAGIDGEEAVDDMLNSKIEPF"
     gene            complement(32488..32560)
                     /locus_tag="PLL88_02315"
     tRNA            complement(32488..32560)
                     /locus_tag="PLL88_02315"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32524..32526),aa:Glu,seq:ttc)
     gene            32760..33869
                     /locus_tag="PLL88_02320"
     CDS             32760..33869
                     /locus_tag="PLL88_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOJ00428.1"
                     /translation="MKNPKNLVLIIRFSGLILFSLLGIYLGNLIAKSFDITVITNNYA
                     MIIGGVVLGALGFFAFPYMTIYPVQTIRKSLEETPGQTLLVAMAGLIVGLIIAALISI
                     PIGVLPAPLNSILIIFALVLFSYLGVTLSVSKQEEIRELLSFARREKKVGSSSKKTGG
                     AGWNKDCSILVDTSVIIDGRIADIAKTGFLPGTLIIPHFVLNELQYIADSAESLRRQR
                     GRRGMEVLSELQKDRNLNVVLSDMDVENVREVDEKLVVLARQMKAPILTNDYNLNRVA
                     ELQGVQVLNINELANAVKSILLPGEELTIKIIQEGKEQDQGVAYLDDGTMVVVEDGRG
                     YLDQEIVVTVTKVLQTAAGRMIFSKPGGANGIDKE"
     gene            33877..35274
                     /gene="cysS"
                     /locus_tag="PLL88_02325"
     CDS             33877..35274
                     /gene="cysS"
                     /locus_tag="PLL88_02325"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOJ00429.1"
                     /translation="MGLRVYNTLTREKEAFRTIQDRKISMYVCGPTVYANAHVGHAMS
                     ALVFDIIRRYLEYRGYEVRHVMNYTDVDDKIIQRAAALNKDPRALAQGYIDKFDEHLR
                     DLNVLPATYKPRATAEMPNIIKFVENLIEKGYAYVVNGDVYYRVRKFNRYGKLSHRNV
                     DEMRSGARISVDEHKEDPLDFALWKSAKPGEPSWESPWGPGRPGWHIECSAMSMAHLG
                     EQIDIHGGGNDLVFPHHENEIAQSEALTGQPFATYWMHNGMLQFSGEKMSKSLGNLVT
                     IEDFCKDHPADVLRIMVLNSGYRNPLTFNDEVIDQAQKGLERLQSALKDALPGAEGAP
                     QASLEALAAQITATQQGFEVCMDDDFNSAGALGNLFDLVRVINQVRADGATDEQLQAA
                     QAVLSELSGVLGLRLQATRQEHAADAFIDMLVALRNELRTKKEWALSDRIRDELAEQG
                     VIVEDSARGSSWHWK"
     gene            35271..36014
                     /gene="rlmB"
                     /locus_tag="PLL88_02330"
     CDS             35271..36014
                     /gene="rlmB"
                     /locus_tag="PLL88_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOJ00430.1"
                     /translation="MREWITGRNPVYEVLRANRREFFRLWLAEGNLEQGGHLQDALTL
                     ARQKKIPVERVPRTKLFEISANHQGLALQVGGYRYASLPEMLALARNRAEAPFILLLD
                     LIQDPQNLGTLLRCAEGFGVHGVLIPSSRAAEVTPAVVNASSGACEHLLIAQMNLAQS
                     IEVLKEENIWIYGLEYSDQAVPIESVDFHGGVGLVVGSEGSGLRSLVRSKCDRLISLP
                     RRGKVDSLNAATAGAIALYVIQHARQKNL"
     gene            complement(36009..36641)
                     /locus_tag="PLL88_02335"
     CDS             complement(36009..36641)
                     /locus_tag="PLL88_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOJ00431.1"
                     /translation="MQPTWKAVLFLFLSVLTILALSGCSDGKTGSIETFGAKDNLTVG
                     TLAALQIVVAIVMLVSLITLIIPGIPGITFIWLAALIYGLVVGFEKPGGIFIGIISAL
                     MLLGNVVDNLMMGAGARKGGAPWLTTLATVVAAVVGSIAFPPFGGVIAAGVVLFVLEA
                     IRLKNSEIALKSTGEMLKGFGCSFLVRFLIGMIMIGLWITWVVQAGQWLL"
     gene            complement(36678..37943)
                     /gene="serS"
                     /locus_tag="PLL88_02340"
     CDS             complement(36678..37943)
                     /gene="serS"
                     /locus_tag="PLL88_02340"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOJ00432.1"
                     /translation="MIDIRLVREEPQTVREGMQKRNLDSSAVDELLAIDEQRRELLQK
                     VETLKAERNTVSREIGKIKDQNEREAKIADMKVVSDSITALDETIRAIEEKQQQLIMT
                     IPNTPLPQVPIGKDESENVIIKTVGEKLPFDFEPLPHWELGEKLGILDFERGVKITGS
                     RFYVLSGLGARLQRSLIAFMLDLHVRQGYLEQYTPFMVKSTTLFAAGQLPKFADNLYK
                     DHEEDLYMIPTAEVPLTGLHMDEFIDAEKLPLRYTAYSPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYTYCKPEESMHEFERMAADAEETCQQLGLTYRVLQLCTGDISFNACIGYD
                     VEVWAPGCQEWLEVSSISNVGSFQARRANIKFKDAEGHKDYVHTLNGSGLGLPRTLIA
                     ILENYQQADGSIRIPEVLQPWMGGATVIR"
     gene            37942..38130
                     /locus_tag="PLL88_02345"
     CDS             37942..38130
                     /locus_tag="PLL88_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00433.1"
                     /translation="MIALLGLIEKPLFLQDEGALVVPPAFAGDDRPLWAVTAASVLLT
                     MRAHPCEGSPLMVSDRLG"
     gene            38216..38830
                     /locus_tag="PLL88_02350"
     CDS             38216..38830
                     /locus_tag="PLL88_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ00434.1"
                     /translation="MDEQTLVQAAQKNDLDAFNQLVLAHQQVVYNVALRMLNDLDTAD
                     DATQQAFISAYQKIHTFKGGSFQAWILRIVTNKCYDLLRSGQRHPTRSIDDASDDPDV
                     DWESAALMRDDAPLPEEQVAQKELEAAVQHCIDELPREFKAVVILVDLQGMDYQEAAE
                     IVRSPLGTIKSRLARARMNLQDCLHKFKELLPAQYRSESEGSHD"
     gene            38823..39713
                     /locus_tag="PLL88_02355"
     CDS             38823..39713
                     /locus_tag="PLL88_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00435.1"
                     /translation="MTDVTRKEWLLLSRYLDGDLSAEQVRRIEEKLRSDVALRQAFER
                     LLRTRQVLRAVPQRKAPRTYLLSNVMVRVERQRVLPQLVWRYSSIAAALVATIALLLQ
                     VFSPRASQASPLSASAQPEMMVLTAPDALDAEPTEQPLIIFWNPGAYGMGGGGEGPST
                     SMAPAAPLEQTDSYGVSGGASTTEDTSSDQGMFAKEAEEVVPEMADESAVAQEQALPE
                     EQALSAAASPSSNPILGIAPPEKRGSLQEFSAEENLQPRDSVGEGSALNFGVIALLAL
                     FVSIVCGLVAFILHRKQQKQ"
     gene            39766..>40984
                     /locus_tag="PLL88_02360"
     CDS             39766..>40984
                     /locus_tag="PLL88_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOJ00436.1"
                     /translation="MVQKRRSSASKSRSTAKNGKKVSRRSAPLRKGLKDLSPERKLDI
                     WGIGLTVLGLLLLLSFVTSQEGNVTGAIVLFLYRIAGYGAFLFPLVCLLIGVWLIFRN
                     EDRLPMISSGKLVGIIMLFFNLLTWIHLLTGGGWDLAKAGGGGGYIGAFFENLIVRAV
                     GQVGAFVVLLGSLLMAVALTIDTAIPDLLRGVKGALAKTGKQVAAQTTQLTQKVAAPK
                     TSTPKTERRKAAESSSIDLEGFTPISEAQHARPSATKQKGRAEREQPPPARPRTQPAP
                     KDAATVQLGLRAQVASPVWKLPAMADILNLANKNENDGAIDKERARIIEETLASFDAP
                     GRVVEIHRGPTFTQYGVEPDLVQTRNGPTRVRVSKINALSDDLALALATQRIRIQAPV
                     PGKHYIGIEVPNSE"
BASE COUNT        10080 a         9677 c        10625 g        10602 t
ORIGIN      
        1 agcaaaactg gataatctgg ctggacatct cttcattgcc cctcaatttc ttggttttct
       61 tctcttcgtt gctatcccaa tactggcagt gattatcttc agctttcaag atcgcaattt
      121 gttgacaggg aagtgggatt ggataggttt tgataattat tcattcatgt ttaccaaaga
      181 cccttatttt tataaagtca ttggcaacag cctgatcttt tcatttggtc tgataatcat
      241 gaacgtatca ttagccctgt ttcttgcctt gattttgttt caaaacaagc ggggtatgat
      301 ctttttccgt accttgttct tttcgccagt ggttacctca acggtcgcct gggttctggt
      361 ttggagcttt atgttgcaaa gtaatcaggg taccatcaat cagtttttga agaccattgg
      421 cattcaagga cctaactggc tgggcagcac acaatgggcg atgttctcgg tgatctttat
      481 tcgtgttata aaaaatgtcg gattgaacat ggtgattttg attgcagctc taaaagatgt
      541 tccgcatgaa ttgatagaag cagcacgcgt ggatggcgca aatatgtttc agtgtgttag
      601 aaagatcatc attcctttaa tagctccaac aattttactt gtcattatca ttacattaat
      661 cggttccatg aatgttttcg atcacattat gttgttgacg gcgggggggc cttctaattc
      721 cacattggtg ctcgcttatt atgtctacta tcaggcattt ttgacaaatg agattgggta
      781 tgccagcgct ctggcggttg ttctctttct ggttgtactg ggattaactt tttttcaatg
      841 gaatatgcgg aagaagtggg tgtcttatga acaataaaca atatctcccc ccattgattc
      901 caaagggatt tcgcaatttc ctattctatt tcggattact gcttcttgca gttcccttta
      961 ttttcccctt ctggtggatg gcaaccagtt cacttaagac ttataaagag atatttgcgt
     1021 atcctcccac tcttcttccg ggatcctggc atttcgaaaa ttttatcact gcttttactt
     1081 atcaaccttt tgccaggcag tatctaaaca gtctttatat ctctctggtg gtgactgctg
     1141 gagttttggt tatttcttct ctttcgggct atgcttttgc gagaattaac ttcaagggca
     1201 aaaatttcct ttttatgttg ctgttgagtt ctcttatgat gccaccagaa gtgaccatta
     1261 tcccgaatta tttgatcatg aataggcttg gatggacgaa tacgcatatc cctttgatca
     1321 taattcctat tttgggagca gggggagtgt ttgccacttt tatgatgcgc caattcttta
     1381 tttctttgcc gaaggatatg gaggatgctg ctaaaattga tggcttggga tggtggggga
     1441 tcttctggaa aattggactt ccattggcaa aagccccgct ttctgctgtt gccatcatca
     1501 catttttgaa cagttggaat agtttccttg agcctttgat ttacttgaac gataccaaaa
     1561 aatacacaat accacttgca ttaacgggat atattgatgc atatggagca cctatgtggt
     1621 ctgtacaaat ggctgcgacc acgctctctg ttttgccggt tttgatcttc tttattcttg
     1681 cacagcagca agtggtagaa agttttgcaa tgtcgagcgt aaaaggttga aaattcgcag
     1741 gagaagtaat tatgagatca gaaaaaatct attccattgg tgtgatcgga ctgggtgtgt
     1801 tcggcgctag gcatgcttcg atatgtttga accatcccaa ctttcggctg gttggtgttg
     1861 ccgatgtaaa aggggaatta gttgaaagct attcaaagga atttggagtt ccaggcttta
     1921 ctgattaccg tggtttattg gctgtgcctg aaatggatgc tgtaactatt gctgtttcag
     1981 atcagtttca tcatgacccc tgcaaagatt cagctcagaa gggtattcat atattcctgg
     2041 agaaacctat tgccgatacc agcactcacg ccaaagaaat attggatgct gttcgagagc
     2101 atgatgtgaa actgatggtt ggtcatacat tgcgttttga tccgcgttat ctggcgatca
     2161 aacgggtcgt tgatgaggga aagctgggag agatcgtcca tgtttacgca aggcgaaatg
     2221 cgaccgtgaa aagtggaaga aggttgcagg gcagggtaga tgccatgatc ttccaggggg
     2281 ttcacgatat tgatttctta cagtggctta ctggttcaaa agtatccaag gtatatgctg
     2341 aagcaacttc taatattttg aaagatctaa aggttaacga cacaacgatg gcaaccttac
     2401 ggtttgagaa tggtgtgatc ggactgcttg agcaatcatg ggctttacct gctgatatgc
     2461 cctccccact ggatgtggaa ttagaggtag tcggcacaga agggtctgca tatttggatc
     2521 ttcgctcgca atcgatttcc attgtatcgg atggaaaact aaaccaacct gatctgattt
     2581 acggtcttcc agatcagcac tttttgttcg atgaatacaa tcatttagca aaatacttga
     2641 gcggtgaaat tgaagcaagg gtcagcggag aagaagcgtt aagtgcatta gaagtggcag
     2701 aagcaatcat gcattctgcg aaatctggta accccattct gttggcttaa attaaacctg
     2761 gagatgaatg atgtcacgat tcgtaaatga actgatgaaa gcacgcaagg aaaaaaagct
     2821 tgtattgatt gcaagtcttc cacgcaatga tgcagaactt gctcgcgcgg tgctggatgc
     2881 cggagccgat gcggtaaaaa ttcacatgaa tgtacatcat catgcgagca ataccacctt
     2941 cggctcactt gatgaagaaa gggaaaatct cactgcgatt cttaaggtat cggaaggaaa
     3001 actcacaggc attatgcctt ttgctgctcc tgtaaatgct cccgatacgt tgaaagaact
     3061 ctccgatatg gggtttgatt tctattcgca atatttatcc catgcagttt ctggatgctt
     3121 cccctcttct gaaaaacctg cccggatgtt ggcattagcg gtcgatgatc caattgaatt
     3181 agccagtggt ttggatcgaa tgcctatgga ggtttgcgag ctatccatta tggatggaaa
     3241 tacctatggt caaccgttca cttatcatga tcttttacga tattctgctg tgcgggtaag
     3301 gaccgggaaa ccgcttgtgg tcccttcaca acatttgatc actcctgagt ccgtgccgga
     3361 tttgattgaa attggcatgg atggattgat gatcggtgcg gtggtggcag gatcgactgc
     3421 ggaaagctgg ggaaaaagtg tcagggcttt tagaaaaatg atcgataaaa aataatgctg
     3481 gatttcccct taaatcgcct gccgttgcgt ttaatgctat ttacgaatgg gtaaatagca
     3541 gagaatagtc agtagccagg atgacagttg ctcccaaaat gatactgttt tccccggatt
     3601 gaccaatttc tattttggta ttgaggattg gtttttgcaa ggaatacttt tgagcggaat
     3661 ttcggatcgc atttaaccag ggttctccaa aacaggtcat gtctcccaca aagacgatct
     3721 tctgtatgtt caaaatgctc acaacatcag atataaccat tccaatataa tgcgcagtat
     3781 cgaagacaag tctttgtgcg aatggatcat tggcattaaa tgctttttgg atagcggcca
     3841 aattgatttg ttttggttcg tgaggcaata tgctttccgg gaaatctttg atacccgccc
     3901 tggctttctt tatcagtgcc tgtgcactcg caactgtttc aaggcatcca tgattgccac
     3961 aacggcagag ttctcctcct tcttttacaa caacaatgtg tccaatttct cctgcacatc
     4021 caccatctcc ctggaaaagc gatccatgga tgataattcc ggatccaatt ccatgtcgaa
     4081 tattaattag aaccatactc tcttcgggag aatatccctt tccaaatgtt ttttccccaa
     4141 ttgctgcagc ctgactgtca ttaagcacat aaaccggtaa gttgtatcgt tcttgaagta
     4201 aatgggtaag agggaggtct ttccagttta gatttactgc attgatgatg accccttcct
     4261 gtgtatttac cagcccggga gtgccaacac caatccccaa gagaggatgc gttgctgcct
     4321 ttttgattaa acgatcgaca atctgataga gtatttctaa agcatcatcg ccatcataac
     4381 cctcaataga catggcgatt acatcgcaga tcacacctcg cagattgaca attgcccccc
     4441 ggaattcatt atgagaaaga tcgagaccga tcatccaccg ggaatcctca acaagactta
     4501 aaagaatggg attttttcct ccgtaagatg ttccaacacc gacctcctct accagacctt
     4561 ctgtgatgat atctgaaaca atgcttgata cggtcgttcg tgttaatttt gtgacgcgag
     4621 caatctcagc tctgctaatc tgctcgtggg cgaaaatggt tctcaataca agggcgcggt
     4681 tatgttcttt cgtatgctgc cgagttgctt tctccatcaa aaactccaag cataaacaca
     4741 ttattagttg agtgtactaa ctaaaggagt ataacaattt atacctatct gtcaatataa
     4801 aaaggttttt ccataaaaca aaatagcatg atttataatc aaacaaaaac gagggaaaaa
     4861 tgaaagattt cttgtggtgg cgcgatggtg tgatctatca gatctaccct cgctctttct
     4921 tggatacgaa tgctgatggt attggcgatc tgcctggtat tatccaaaaa ctggattatc
     4981 tcaaagaact tggtgtagac gctctgtggc tttctcctat ctacccttcc ccggatgtgg
     5041 attatggtta tgatgtgagc gattataagg ctattgctcc ccaatatggt accatgcaag
     5101 atttcgagac cctgctcacc caggcgcata aacgcaatgt gcgcatcatc ttggacctgg
     5161 ttctcaacca cacttccgat cagcatccat ggtttatcga atcgaagagc tcgagggata
     5221 atcgttaccg ggattactac atttggaagg atccgaagaa aaatggcgct ccccccaata
     5281 actggctttc tgttttcgga ggatctggtt gggaatttga tgcccatacc ggtcagtatt
     5341 attttcacca ctttttcaaa gaacaacccg atctcaactg gcgaaatccg acggttcgta
     5401 aagagctgat agatgttttc cgtttttggc tggataaagg cgtagacggt tttcggctgg
     5461 acgtcttttc cgcctatttt aaggatgctg actttcggga taatccaaaa aaattgattg
     5521 gcttgaggaa gtatgaccgc atgattcacc tgtacgatgc agatcgccct gagatgctcc
     5581 cgcttctgca agagattcgg gctttgttga atcgatatcc tgagcgatat gcggtgggcg
     5641 aaaccttcat agctacgttt tccaaagcag ccggttattg tggtgataac cttttacatg
     5701 ccgcattttc gtttgataaa tttatgaatg gaccgtggaa agccaaacga ttcttgaggg
     5761 caattcaaaa atgggaatct gcattggaag ggaaatgctg gcctaattac gttctgaata
     5821 atcatgacgt aaaacgatct gcaacccgct ttgggcaggg agaggacgat gaacggttaa
     5881 aagtggcgat tgccctgttg ttgactctgc gcggaacacc gtttctttat tatggagaag
     5941 aaattggcat gcgtgaaagc aggtttaaga aatccgagat tctggatcct gttggcaagt
     6001 tctattatcc attttataaa gggagggatg gctgccgcac acccatgcag tgggatgctt
     6061 cagagcacgc tggtttcagc acggttaaac cctggctgcc tgttcacccc gattatcttg
     6121 agcgcaatgt aagcgcacag caaaaggatg accggtcgtt gctcgctttt tataaaaaac
     6181 tgatgaattt gcgcaaacga catgctgccc tgatgcaggg tgatttttcc ctggtgcccc
     6241 attgtcctgc caatactctc gtttatttcc gtagtgcagc cgaagaaagg ctgatgattg
     6301 cattgaactt tgacgggcgt tcaaaaaaga ttgcactgcc tgctcaagat gccaaccagt
     6361 atgcgttgct gctttctagc atggagggta agccgcaaca gatagcaggt gatgattatt
     6421 tgctgagcgg gaatgaagca gccatcttcg ccattaaaag aagctagctt gccttagaat
     6481 ggtgcttttt cagtaatttg tggtggattc atcactttgc ttggcgggca agactggata
     6541 tactgaccaa aaaagtaaga gcagacgctt atgacaagaa tacgagaaga aagaaaacgc
     6601 tgggagcagg aaaccttccg acccgtaatc gaaaaatatc ctgaacggca gccggagttc
     6661 agcacccctt ccggcattcc cgttccgccg gcggcgattc ctgcggatta tgactacatg
     6721 gagaagctgg gctttccggg agagtatcct ttcacgcgcg gggttcagcc ttcgatgtac
     6781 cgggggaggt tctggacgat gcgccaatat gccggttatg ccactgccca ggaatccaat
     6841 gaacgctacc gctatttgct gcagcagggg caaaccggtc tttccgtggc gttcgacctg
     6901 cccacgcaaa tcgggtacga cagcgatgaa tccattgcag ccggagaagt gggtaaggtg
     6961 ggggtagcca tatcttcgct ggaagatatg gagctccttt tccgtgagat tccgctggat
     7021 aaagtatcta ccagcatgac catcaatgcc ccggcggcgg tcctgcttgc catgtacgtg
     7081 gctgtggcaa aaaagcaggg tgtggaaccg ggtaagctgc gcggcaccgt tcaaaatgat
     7141 attcttaaag aatatgttgc gcgcggcaca tatatttttc cacccaaacc gtccatgcgg
     7201 ttgataacgg acacttttca atactgccag cagaacattc cgcgctggaa cactatcagc
     7261 atttccggct atcacatccg cgaagccggt tctaccgcag ttcaggaggt cgcttttacc
     7321 ctgtccaacg ccatcgcgta tgttgaaaca gccgtgaaag ccgggctgca agtggatgat
     7381 tttgctcccc agctttcgtt cttcttcgcc tgtcataatg attttcttga agaaattgcc
     7441 aagttcaggg ctgctcgccg attgtgggct accataatga aagaacgttt cggatcgcag
     7501 aacaccaaca gcatgcggct gcgtttccac acccaaaccg gcggttcgac cctcaccgct
     7561 caacaacccg agaacaatat tgtgcgcacc accattcagg cgctggctgc tgtattgggg
     7621 ggcacccagt cgttgcacac caactctatg gatgaagctt tgtggttgcc aaccgaaaag
     7681 tcggtgcgca ttgccctgcg cacgcagcag atcattgcac atgaatcggg tgtggctgat
     7741 tcggtcgatc cgctagccgg ctcgtatctg gttgaatatc tgaccgatga aatcgaaacg
     7801 cgcgccaaac agtatattcg gaggattgat gatatgggag gggcgctggc ggctattgaa
     7861 aacggcttca ttcagcgcga gatccaggat tccgcttata cctatcaaaa gagtgttgaa
     7921 gaacaccatc acgttctggt gggtgtcaac gattaccaga ttgcggagac gatcgatatc
     7981 gaagcgctgg cggtcgatcc tgcgttagaa cagggtcagg tcgaacgttt gaaaaaattc
     8041 aaagaaaatc gtgataccgg caaggtgcat gagttgcttt cggttctgga acaaactgcc
     8101 aagaccgatc aaaacttgtt gccattgatg attcattgcc tggagcagaa actgacattg
     8161 ggcgaaattt gtggtgtgtt gcgcacggta tggggcgaat atcagccccc ggtttgggct
     8221 tgacgtttta aaaaagggaa aaagatggaa ataaaaaaga tcaatcatgt agcagtggca
     8281 gttgaggaca tcaccagagc aactgaattc tggcaatccg tattgggatt gaaactggat
     8341 cacgttgagg aagtggaaag ccagaaagcg aaagtggctt ttttcccctg tggtgaaagc
     8401 gaggtggaac tggttgaacc caccgatcct gaaagcggaa cggctcgatt cattcaaacc
     8461 cgcggtgcag gcatccatca cctttgcctg gaggtggatg atatcgataa agcactggaa
     8521 gaattaaaag caaaggacgt acgcctgatc gatgaaacag cggttgttct ggaagggcga
     8581 aaaatggctt tcatccaccc caagagcagc gggggcgtgc tgattgagtt atatcaaatc
     8641 atttaaacaa gaaaagggtt cgccagtacg cgaacccttt tttgatcatt ttacggtaat
     8701 catgctttcg tcttcttctc tggcggtttg aataccgcgt tccacatcca ccctggtaag
     8761 cacgatcatg ccgagaataa agaagatgat gagcgagacg atgctcaggc ggctctcgcc
     8821 catcagcgtg gacaccagac cgaacaaaag tggaccggcg atggaagaga atttttcaaa
     8881 aacactgaag aaaccataaa attcagccga tttactctta ggcatcatgc gaccaatgag
     8941 agaacggctg agtgcctggc tgcccccctg caccatggct acaccgaacc cgagcgccca
     9001 aaaatgccag ggaacgctca tgaagtaacc cgcgatggcg atcatagtat aaatgacaag
     9061 gctgatatag atcgatttct tggctccgat cttttttgcc agtgctccga atgcgatcgc
     9121 aaagggagca gccacgaatt gaaccatcaa gagcgtgcca atcagcgtgg tttgcccgat
     9181 cccgatttca gccccataga tcgttgacat ggtgatgatc gtaccgatcc cgttggcata
     9241 caggaaaaac gcgaccagga atgtgaacag gtcgcggtaa tttttgatct ctttgaaagt
     9301 cttgcccagg cgctgaagac tgacctttag tgggttcaat ccttcttcat ccttttggat
     9361 gcggcgcggt ggttcggaga tgtgccggaa caatggaagt gaaaagatga accaccagac
     9421 ggcaacgctc agaaaagaca accgcgacat aagggtggga tcgatgccgg ggaatagggt
     9481 ttcaccaaac atgatcatcg ctacgttgat ggcaagcagt aatccaccac cgatatagcc
     9541 catggcgaac ccctttgagg aaacctgatc aatctcatct gaggatgcaa cgtgtgggag
     9601 cagcgcgtcg tagaatacca gagatccaga aaagcccatg ttcccaaaaa tgtacagaat
     9661 agatgccagc aaccaatcac cggttctgac gaaatataaa agagcggttg cggtgacacc
     9721 cagccccagg aatatggaaa ggaacctttt cttgtttccc ttgaaatctg cgattgcccc
     9781 cagaattgga cctaacaatg ccaccagcag agcggcaaat gaggttgtga atccccagcg
     9841 cgctgtggcg atattgggtg ccagggttgc accggctacc gaggagtagt aaaccggcag
     9901 cacggctgcc atgatggttg tggcaaaggc agaattaccc cagtcataca tggtccatgc
     9961 ccatattgct tttttcttct tcttatcttc catcccagct ttctccttta tcagtgtaaa
    10021 ttattccaga tcatttctta atcatatact gaaattgaat aattatgctc ttgttaaacc
    10081 aggcttaaca aacggctgct cattttgagt gggagtgctt aaaatagaag gcgggggtgc
    10141 aactgcactc ctgccttgtg aaagaaaact ttacttgatt tattgatctt gcctgcggtt
    10201 gacctttcgc agaaattgga tcagctcaac cagtgtcccg atggggatga ccaccagcaa
    10261 gaaaatgaat acgatctttg caatctgtat ccacaactct gaggggaatt caaaggggaa
    10321 aattgtatat aggaagtagg tcgaaagcca actgaacaca ttcaagatga cctgcatcag
    10381 gtgatggaac cagttcacat cgaaaaaaat gaaaatgaaa ttgacaaaaa tcgtcgcact
    10441 cagcgataaa cggatcaccc acaggcaatc ttcaaacgta ggtaaaagaa aagggatgtt
    10501 ccaggataaa atattgttga aaacatagat aagagcggcg tttaccagca tggctatgat
    10561 gtaaccgaat ctttttccgc ttgaagcttg tttttccata ggtgtatttt cctttcaatc
    10621 agtatacgca aagaatagag gaacgtttct taaaaatcga gcatcaaggc atccactgct
    10681 cttccgcaga tgctgagccc tttttgttgt tgacgttttt cctttcctga tttatagttt
    10741 agcaagttaa ctgttaagtt agttaacgaa agggtcaagt atggcagatg acacattgct
    10801 tcaaaaaacg gtcgagagct tttgggatac catccccccg gtttgggggc gggtgcgcga
    10861 aaatgcccgt ggtaacgcta tccaagactt caaccttacg ctgattcagt ttcatatctt
    10921 acggcatatc tggcatggca tccgctcggt gagggaattg gctgaaaagc agcagataag
    10981 ccgacctgcg atcagccagg tggtggaggt gttggtgtta agaggattgg tggtgcgcaa
    11041 acaggatacg cgtgatcgtc gctgtgtgca gttggaactt actgccaccg ctacaaagct
    11101 gctggaagct gtcttcagca agaatcgtcg ttggatggct gaaaaaatgg tttccttgga
    11161 gcaaagcgaa ctggaaacga tcatggctgc tatggagata ttaaaaaaga catttgatcc
    11221 ttctgtcaga tgagttaatg gtatggctac aatctcgaaa attttaaaat ttgtcaaacc
    11281 ttattggaaa ttagctttgc tttccatgct gatgttattg ctgatggtgt tgagcgacct
    11341 ggctatcccg cgcctgatcg caaccatcat tgatgatggc attaggcaga atcgcatgga
    11401 tgtgatgtgg cacacttctc tcatgatggt gggcatcgct gtcatcaata ccgttgctgc
    11461 cgttctcaac aacatctttt cagtgcgggt gggcgaaaag gtggcgcgtg atatgcgtga
    11521 agcgctgttt ctcaatattc agcaattttc ctatggagat ctggaccgct tcagcactgg
    11581 caaattgatg gttcgcttga ccagcgatat tgccgctgtg caattgatgg ttcgggtgtc
    11641 tctgcgcatt ggtgcgcggg cgcctttgct gatgatcgga agcatcattc tcatgttttt
    11701 gaccagtgct cgcctggcga ccgctattat cccgattttg atcattacct ctgccattat
    11761 cattttattc agcataaaaa tggaacctct tttccgtgct gtgcagcaaa aattggatca
    11821 tctaaacact gtgctgcagg agaacatcgc cggtgcgcgc ctggtgaagg cttttgtgcg
    11881 cgccgaccat gaatcgcaac gttttggaga ggtgaacgaa gatcttacct tgggtacggt
    11941 aagggtcatg cagttcatgt catccatgtc gccggcattg accattttcg tcaacatcgg
    12001 aatgatcctg gtaatctggt atggcggatt gcagaccatt cagggcgagc ttcagattgg
    12061 tcaggtggtt gcttttacga attatttgtt gaccaccatg acccctctca tcctgatggc
    12121 aaatctctcc aatacctggg cgaacggact ggcttcagcc aaacgcatta accagattct
    12181 ggatgttgag cccgaaatca cctttcccac acaggaagca caaatacctc aaggttggtc
    12241 caatgctgtg gaatttagcg aagtttcttt tcattacaat ggcaataaca atctggaggt
    12301 gttggaaggc atcaatttgc gtgcacgccc tgcccaaatt gtggctgtgt taggagcgac
    12361 cggctctgga aaatcttcat tggtcaacct catcccccgt ttttatgatc caagtcgtgg
    12421 agaggtaagg atcggtgatt tgaatatcaa ggtattgaaa gaagcgacgt tgttgagtca
    12481 aatcggcatc gtgccgcagg aatccatcct gttctccggc acagttggtg agaatatttc
    12541 ttatggaaaa gcgtgtgcaa cggatgagga aattgaaatg gcagcaaaag ttgctcaggc
    12601 gcatgatttc atcatgagtt ttccaaaagg gtatgcctcc tatattgaag aacgcggtgc
    12661 gaatctttcg ggtgggcaaa aacagcgcat cgccattgcg cgagcgatca tcacccgacc
    12721 acgtatcctg atcctggatg atgccaccag ttcggtagac gtggaaacag agaccaagat
    12781 tcaggatggt ttgcgggaat atgtgcaggg ctcaaccatt tttatggtag cacagcgcat
    12841 cagcaccgtg ctgaatgccg ataggattat cgtgttggat aaagggcgcc ttgttgccca
    12901 gggttcgcat gcggcactga tgaagaagag ccgtatttac cgagagattt atgaatccca
    12961 gcttgggaat gggttagcta gttatgagaa agaggcgttg caatgaatcg cccgactgat
    13021 gtccgttcca gcatgcagcg cagacctatc cattctgttc ccatcgaaaa ggcgaaagat
    13081 ccaagccgtt ccatagcgcg cttgttgccc tatcttaaaa gctataagcg gcagatcggt
    13141 tgggtgattg tcttcattgt gctgtacacc ctgctgggtc tgataggccc atatctgatg
    13201 ggtgtggcga ttgacatttt tatcgctgag aaggatgtgg caggattatt gcgaacggcg
    13261 ctgtggatgt tgctcgttta ccttctcagc aatggtttga atgtatgggc gaattggatc
    13321 atggcgcgcg tttcgcaatt tgccttgcga gatctgcggc gcgatctttt cgaccatctg
    13381 caagcacagt ctatgagttt tttcgacacg cattcggcag gtgggttgat gagccgactg
    13441 accaatgata tcgatgcgat caaccaggca atttcacaaa atatcacctc gctgatcgca
    13501 agcacccttt ccatgggagg gatcgtggtc gccatgttcg tcttggatcc atggctggca
    13561 ttaggtagct tgcttgtcat tcccatcatg gcgtggttta ctcgttttgt tgccaaatac
    13621 acccgccggg gtttccgaga attacagaaa gaccttgggg aattgaacgc aattgccgaa
    13681 gaatctatca gcgggcagaa ggtcgtcaag gcttttcaga ggaatgaaaa tgtcgtcgcc
    13741 gctttccgaa aacagaacga agcggtctat gcggctgggg tttatgccaa ttcctatgct
    13801 ctgttattga tgccgctcac ccaggtgctg ggaaacatct tcgtcatcgt gctggctacg
    13861 cttggaggat ggctggcttt gcaggggctg gcaagcgttg gaatgatcgc cacgttcatc
    13921 aactacgggc aaaattttgt ctctccgctg cggcagatcg ccaatcttta caattccatt
    13981 caagctgctt tagctggcgc cgagcgcgtt tttgaggtca tcgacaaccc gcctgaaata
    14041 caggacgcgc ccgctgccat ttcgctgaac gagatacgcg gagacgtgtt gtttaaaaat
    14101 gtggatttta gctatatccc ggggcgaccg atcatcaaga acaccaccat cgaagccaaa
    14161 gccgggcaga ccattgctct ggtgggacca acgggagccg gaaagaccac catcatcaat
    14221 ctgctgaaca gattttatga gatccagggt ggctccatca gcatcgatgg gcatgatatc
    14281 cgctcggtaa agaaagccga tttgcgaaag agcctgggta ttgtgctgca agataccttc
    14341 ctgttttcag ataccatcat ggagaacatc cgctatggac ggctggatgc gacggatgaa
    14401 gaatgcatac aagccgctga aatggcggat gccgatcatt tcattcgcca attaccgcat
    14461 ggctaccaga ccaacctctc ggagcgagcg ggcaacctca gccaggggca gagacagctc
    14521 atcgccatcg cacgtgctat tttggctgat ccagccatct tgatcctgga tgaagccacc
    14581 agctcggtgg atacgcgcac ggaggcgcgc atccagaagt cactgttacg cttgatgaaa
    14641 gggcgtacga gcttcgtgat cgctcaccgg ctcagcacca ttcgggatgc agataaagtt
    14701 gtggtgatca atcagggtga ggtggtggag cagggcaccc atcaggagct tctggatcgc
    14761 aggggttttt atcatcattt gtacataagc cagttcaagg gcaaagctat ctgatggtta
    14821 agttcggaag ccctttcttt tgaaagggct tcctcagtaa aggggctgat agtgatcggt
    14881 ttatttagag agaatcagaa cctgcgtccg gcgttggggc tacgttctcc gcgggtttca
    14941 tcctggtatt taaaaacccc aacgcggcga tcaagcataa tattccaccc ccgtaccata
    15001 cccattgcgg gtggcttgat tcaagcccat atccggcagc caggggtcca aatgctgcgg
    15061 aaaaaccgta gctgatattg agaaatgcca tataacgacc gcgcatatct atcggcgcga
    15121 actcagccgc tataacctgg tacaggggag cgctgatcat ttccccgatg gtaatgatga
    15181 tcattgccac tacgaagtag gcgtagctgt tgcccgatcc gaacatgctg aacccaatcg
    15241 cgtacagcag ggttcccagg aacatcatga gtggctgttt gtatctggct gtgatgcggc
    15301 tgatgctgaa ttggaatgcc accaccatca ttgcgttcag ggagagcaaa aagccaaact
    15361 gcttcgctga gatgccgtgc agccggttga ggtagactga aagggtagtg ttgtagttca
    15421 tgtaaaccat catggtcagt acgatcaaaa gaaccagcgt tacgaatact cgatcgcgca
    15481 gcaccatgcc atatccggca aaggtcgttt tgagtgattg cttggcggag tgctcgggtt
    15541 cttccggttt ggtttcaggc atgatcacca acaagaagat gccggtcgca acgctcagca
    15601 cgacgtccat gatgaacagg ggcaagaatg aatgaccgac cagaaaccct gccagaaaag
    15661 gaccaatagc agcggataaa ttgccgatga tgcgcaacat ggcgtaaccc tgcgatcgtt
    15721 tttcagaggg caatatatct gccatcaaag cttgaaaagc cgggttggca aagttcccca
    15781 tgaaagagga gagcagtcgg aatacgatga ataaatccca gcgattggtc agcccaaggg
    15841 gcaaggtgat cagagcgcta ccgaacaatc cgatcaatcc gatggttttt cgcccaaact
    15901 gatctgccaa cgcgcccccg atgcttgtgc caaccacatt gaccatggtg ctcaccacct
    15961 gtatccaacc gatatcgatc agtgagaccc cgaatttttg cgttacatac agggcaagaa
    16021 aaggaaaaag cagcgagctg ccgatgttgt cgacaaacat ccccaacatc acattccaga
    16081 aggtttttgg gaattcgtgg tatgtttttt taatggttgt gagcacgttt ctccagagtt
    16141 tagaaaaata attcaaataa tgcagaaata ctatcataat atttaaaaat atcaaccatt
    16201 aacttaaaca atattgatat aataattcaa tatattaatt atctcgtaag taaaacggac
    16261 gatgaatagc tggaacagcc tctcatcggt gtttggcgtg tgttttttca tacaatgctc
    16321 atttgacgga tgaaagaccg tctgatagaa tggtgcagtt tgacgcgttg tacccttcgc
    16381 gttaattcaa gatcagaaag ggcttgtaca catgaaagtt ttattaattc gagatgtcta
    16441 taatctgggc agggcagggg aggtcaagaa ggtagccaat ggttacggca gaaatttcct
    16501 gatcccacaa aaactggcgg tgatggtaac tcccggcgcg ttgaagcagg tggagaagat
    16561 ccaatcggct gctgaaaaac gccgtgtcgc tctcaatgaa gaaatggctg gcgtggcaga
    16621 aaagatccag gggctcgagc tgtccttcac tgccaaagtt ggcgagaccg gtcacttgta
    16681 tggctcgatc acacagcaaa tgatcgcgga tgccatcgaa gagaaaacca gcatcaagat
    16741 cgatcgccac tggatcgaat cacagcctct gcgcgaggtc ggtctgcaca atgtgaaggt
    16801 tcgcctcacg ttcgacctga ttcccgaagt ggcagtgact gttttgagtg aagaagaagt
    16861 tgaaaaagcg ttgaaagaaa aggcaaagga agaacggaaa gccagaaaag agaagaaaga
    16921 agaaaagctt cccaccatcg atgaaattcc ttccttcacc gaacccaatc aagacgatgt
    16981 gaaataaaac tcaaagagca gccggtctga gagaaatcac agaccggttc ttttttattt
    17041 ttggttataa tctagcacga tgagtaaaac cgtaggacaa atattaaaag aaacgcgtgc
    17101 gctgaaggat atctctttac aagaagccgc cagggcaacg catatccgtt tgcagtatct
    17161 gcaagagtta gaaaatgacc gtccggaatt gctgccgtcc cgagcccagg cgcgcggctt
    17221 tttacgtcta tatgccgcct ttcttgagct ggatggaacg cagttgttga cgttgctgga
    17281 atctccccct ctgccggatg aaccgcttga ggacattccg aaaccagagg aatctcccga
    17341 gttaaaaagc gaagcggaag aaaagaagga agaatccgtt gcagagcctg cccttcaaaa
    17401 accgtctgca cagcctaaga aaacaaacaa agtttctgcg aaactgcaag ccctgatcag
    17461 taaactccct aagtttggaa agaaaaaaac aacatcccca acaccttctt cccaggctga
    17521 agatcaggca cccacgcaac ttcctccagc gtcgaaaaag ggatccaaaa aagaagatcg
    17581 ctccgctggg aagcgcaagc aagcatccaa gaacccggct cgcagagttt ctccccccgc
    17641 tgtgccatcc gaagatactt ttaaagaggt tggatcacaa atgcgccgcc gccgggagaa
    17701 actgggcttg tccctttctg acgttgagca attcacacgg ctgcgccgca tttacgtcca
    17761 agccatcgaa gatggcaaat ttgaagacct gccttcttct gtgcagggtc gtgggatggt
    17821 tgcgaattac gcccattttc ttgagatgga ccaggatgcg gttctgaatc tgtacgccga
    17881 agggctcgaa gcccagcgcc gtgagaagct gcaagaaaaa tggacaacca aggaaccggg
    17941 cattcgcctt tctatccagc tccccgagaa gattcggcgc tttctctcgc ctgacctgct
    18001 gttgggatcg ctggtggtgg tggcgctgtt tgtgttcatt ttttggggtg cttcgcagat
    18061 ctttggcgat aagagtgaaa ccgcgcctac tacagctcct tccatttctg agatgctgca
    18121 aagcacgacg acgccaaccc tggctctgga atccacagcc gggctaacgg gcaccgaaag
    18181 cgatgaaccg acggcgctgg ctatggaaac acaggtagcc acgcagccgg tcgcgccgat
    18241 tgccactgcc aacgccgctc cgcttcagtt atacattgtt gcacaacagc gtgcctggat
    18301 gcgcgttacc gtggatggga acgtggagtt tgaagggcgc atcatcccgg gaaatgccta
    18361 caccttttcg gctcaagagc gcatcttgct gctcaccggt aatggcgctg ccctggaggc
    18421 ttacttcaac caggaatatc tgggcgcgtt gggcaagatc ggcgagatgg ttaatctgga
    18481 tttcagtgct gcgggcttaa gtgttcctac tcccaccatt acccccactg ttttgccgag
    18541 cgcgacacct acccctgctc ccacgtttac cccgactccg cagggtacgg ctacagaaga
    18601 gtgatgcctt ctactccaaa agttcttgtt tgagcgcatg atagaccgct tcggcggtcg
    18661 agtgtgcttc gaggcgtgtg cgcgcttcgc gcaggtgcgt tcgcaccatt ctttccgtga
    18721 cccccagctc ttgcgcgatg ctgcgagccg ttaaaccatt tgccgccagc cgtaaacact
    18781 ggatctgacg ttttgtgagc atgcgatttc tggctatggt cttcacgctt ggtttatgag
    18841 gtgagatttg ctgtatatgc ttcatattcc tgttatgata gaaaatttgt tctatagtag
    18901 aatagcataa aataacaagg gatgcaaggg gtattgattg atcatattct atgaaactgc
    18961 acagaagtta atgaaaggta tcttataatt gtgtccctat gaaaagatcg ttctacatcc
    19021 attcccttgg ctgccccaag aatagcgtgg attctcaatc catggcggaa ctgctgatca
    19081 agcagggata ttccgtcgcc aatagccagc aagaggcagg catcatcatt gtcaatacct
    19141 gcggcttcat ccaacctgcc cggcaagaag cgctgaattt gttgaaagag atcatgcgcc
    19201 aaaagaaagc atcacaaaaa gtgatcgcgg ctggctgcct cacccaacgc gaagcagaaa
    19261 tggtaaagag agaggtgccg ggattgaacg cgctgatcgg cacgcggcgc tggatggata
    19321 ttcttgcggt agtgcgccaa ttggatatgg caactgctca accgcttacc cgaattgccg
    19381 atggcaaact ggaaatggaa gaagagcata tcatcagggc ttccaacctg ggtggatatg
    19441 catatctgaa gatcgccgac gggtgtgacc ggggttgctc tttttgtgct atcccgctga
    19501 tcaagggtcc tcaggtcagc cgtgacattg ctgatatctt gcgcgatgtg catttcttgc
    19561 aggagaaagg ggtacaggaa ctgatcctga ttgcgcagga tgttaccgcc tatgggcgag
    19621 atgtgggcat gagcggaagt ttgcccgctt tattggaaca gatcgtaaag attgccaccg
    19681 ctgttccctg gatcagactg ctctatactt atccgggcga gatcagtcag catctgattg
    19741 cattgatggc agcaaaccct caaattctcc cctatcttga tattccgttg caacatgcct
    19801 ctccgcgtat tttaaagcgc atgcaccgcc caacgggtgt ggaaaaaatt atagccttca
    19861 ttcaagatat tcgcgagcag attccccatt tggcgatccg ctccactttc atcgtgggtt
    19921 ttcctggtga aacggagcag gattttgaac ttcttcttga ttttatccgc tccatgcgct
    19981 tcgaccgggt gggtattttc ccctactctc acgaaccggg cacggttgcc tttcagtatg
    20041 ccgatgatat ttcaccgcag gaaaaagaag agcgcgttca gcgcctggct gaactgcaag
    20101 aggaaatctc tgaacagaag aaccgggaat atgttggaaa agagctggat gtgatcgtgg
    20161 atggggtggg cgatggtatt tcggtctgcc gctcctatcg cgatgccccg gaggttgatg
    20221 gcttggttat ggtgcaggga gaattggaga tagggaaaat aacagcggtg gaggtcaccg
    20281 ctgctctcgt tcacgatctt gtggctgtcc gttccaggtg acgcacaatc ttattctgtc
    20341 ggttccgcag tagcttcgct tgtcgcttca gtggtgatgg tagtgatggc ttgcggttct
    20401 gcggttggac cctggcagta aaccgttgct tcgttcaaca ggtttgatac ttcttcatca
    20461 tattgaatgt tgagtgataa ctggagctgt tcagcggcac tgcaatattc ctcgatgctc
    20521 atgaaatatt ttccatattc ccgcaatccg atgcggtacc ggtcgatggc ggtccagccc
    20581 gaagtgtcgt gcaggtaagg gaaggcggcg taggtctggc tgaaatagta caccacctgt
    20641 gcccaatcca cttcccaata actggcgccg gtcacgtaca ggcgtgccca cgcagcgtaa
    20701 ttcacggcgt catgatccaa cggtgcgaat ttttgtgcca gactgaggta atagagccca
    20761 ccctcgagat caccttcatt cgtgatcttg taggtcgcgc aattgcgcaa tgaagcccag
    20821 atcatgccat ccacggtcag cgtttcatac gaaacgtcct cgtcgcgcag actttttaac
    20881 gtgtccactg ccaggcacca ttcctggtta gccatatccg tttgcgcctg ctggaacagc
    20941 gcttgcgctc cctggatatc tcgcgtgggg gtggcggttg cttgcgcggt tgtttgttgg
    21001 gtttttgtga ggttgataag cacttcacta tatttttcgg tgatatctgg aaaggtgggg
    21061 tagatctgga tcacgtattc aaattgaatg cgcgcgacct cataattacc gctcgccatg
    21121 gcttccattc cgtacttgta gtgggtggtc gctaaagtga gccgttgttt ttcttcctct
    21181 gccttgcgtt gtttgatgcc cgaatagtac cccagagaac cgctgatgag cgctgctatc
    21241 agcacgagca gtaagcccat caaaacagcc cgtacatagc gcggtctccc gccctttctc
    21301 ttgccaccgg ttttgatagc ctgggtgtct tctttggttg ttttctggtc cactttgatt
    21361 tcagcggttt tttctgcgtt attttttgcc atgcgcgtta ttataccgtt cttttctata
    21421 ctagcctgtc tataagttta ggaagacctt cagctctttc cagatgaagg gcagaacggc
    21481 gcctatccca aagaggaagc tcgccatgcc ttgaacgagc aggggcaacc cggcgcttct
    21541 ctgcataacg agccaggcaa tggcgccggc tgttgcactg ccggctatag tgcgccagat
    21601 cgtttgccct aattcgcgcg ttgtcttttt ctcttcctca cctttttccg ggcgcgtaga
    21661 ctttctgtcg cgcaggacga acaggatcaa cagaaaagct gcctggattg aaaaggcgat
    21721 ggaatcagcg agcgcgatgc cggttgcgtc taatctgctg gatagtagcg taccagccaa
    21781 tatgtagatg ccgaaattga tggcggaggc aagaaatgga atgaccgcat tttgctgtga
    21841 gaagaaaatg cgcgacccca gttcgagcaa gcaatgcccc agcaaaccaa ccagaaaacc
    21901 gcgcgtgacc catagaagtt ggtcagtggc ggcagtgtcg aatccgaatg cggtttcaat
    21961 gaacgggcgc aggacgagcg acagtaccac cgtgatggga atggcaaggg caatgatcac
    22021 cctttcaact ttttcgatgg tggcgtgata tcccgcctgg tcttttcttg cgaagagctc
    22081 tgatagggtt ggcaggaggg cggttccaag agcagttccg atcagcgtct cgggcacctg
    22141 ctggatcatc caaccatagg ttaatgcggt gggggcgcct tcactgagat aggatgcgat
    22201 gttgtcgcgg gcgataaaag tgagctggta gcataacatg ctgccaacgc gcggtgccag
    22261 catggcaaga atccttttta cgtagggatc ccgtttgcca agacccggga accacttgaa
    22321 ttggaagcgg aacagccccg ggatgagtac tatcaggtag aggagcgagc cgatcaaaac
    22381 cccgtaaaca attccataca cgcccattcc gtaagcgggg agcgttattc cagcgatggt
    22441 caatccttcc accggtgcca ggatcaaagc tccaaagatc tgtccaagat tgtacataat
    22501 gggagcaaca gcgggcaaaa agaagtgctg gttggcttgc aagcccgcca ccagcagacc
    22561 agccatagaa aagatcagcg ttgctatcag gtccaggcgc atgatgctta ctaccaacgc
    22621 ttgctgctct ggggaaaaac cgggagctat cccgatgggg ctcttaacca gcggttcggc
    22681 gaacacgcca accaccaacg acaaagctgc ggtcaccaga aaagcgaaat tcgccatctg
    22741 gctgaacacc cgccagccat cgctgcgacc ttccttggtc aatacttctg aaagcaccgg
    22801 gatgatcgcc atcgccagcg ctccgccaga aatgagcgaa taaagcatgt cgggcacgtt
    22861 gttggcaacg ttgaacacat ccaactcagc cgatagcccg aactggcggg cgatgattag
    22921 ctgtctgaca atcgccagaa ctttgttcac tgcaaagctc aatgcgatga tgatcgttgc
    22981 gcgagttagt ttgctcaagg tatttccttt ttatttgatt ttaacctaaa gccacatcca
    23041 aaatcatcat gaccgtaaaa ccgatcaaag cgccccacgt ggcgatgtcg gtgttttcac
    23101 catgttgggc ttcaggaatc agttcttcga tcaccacgta gatcatggca ccggctgcga
    23161 aagccagggc gtatggcaaa atatgccgca tggtcaccac cgcggctgcc ccaaggatcc
    23221 cgccgagcgg ttcaacgatg cctgaaagct gtccgtacca aaaacttttc caacggctaa
    23281 gcccatcacg tcgtaacggc acggataccg ccaacccttc cggaaagttt tgaatgccaa
    23341 tgccgattgc cagcacgata gcgcccgaaa gggtagctcc ttccacacct gctgcggctg
    23401 cgccaaaggc tacgcccacc gccaatccct ctggaaaatt atgcagggtg atggcgagca
    23461 cgagcagcac agagcgctgc caggtggttt tgattccttc tgccatctgc acgggcatgt
    23521 ccatgtgcag gtgcgggagg atcttatcga tgatccataa gaccaatcca cccataagaa
    23581 agccgatggc aggtgccagc cacggctttg agctgactga gccggcgatt tcgatggcgg
    23641 gtgctagcag tgaccagaag ctggcagcga tcatgacccc tgctgcaaag ccgagcatgg
    23701 catccagcag ttttttgttt acttcttttg atagaaaaac ccccgctgcg cccagagcgg
    23761 tcactcccca ggtgaagagc gttccgaaca atgcttgaat taccgggttg aattgattga
    23821 gaaatgcaat catcgttaat atctcctgtt tattccgata aaatagaaac cttcctctaa
    23881 ttgtattgga attttgccag gtaaggatgg catttttgga atattggctg tgtgatgaaa
    23941 agcgtggctt tcgacaggct ataataaacc catactttta aacaggatgt tcgtatgcgc
    24001 tcaggaatcc tctatatggt cgccacaccc atcggaaatt tgcaagatat cacggttcgt
    24061 gcgttggatg ttcttcagtc cgtcaaccgg gtgatctgtg aagagcggcg cgttggcagc
    24121 gcaatcttgc accaactgaa agttgaagga aaaacactcg tcgaattgaa cgaacacaac
    24181 gaagaacagc aagcgcagga actcgcgctg gcattgttga acggcgagaa tatggcgttg
    24241 atatccgatt gtggaacgcc cgcttttgcc gaccccggct cgctcctggt gcaactggct
    24301 tacgattatg ggatcagggt ggtaccagtt ccgggagctt catccttgat ggcgttgctt
    24361 tcggtttctc cgttgccttt aaagaaattc tattttgctg gatttctgcc gagaaaagag
    24421 gaagaacgaa aaaagagcct ggagcaattg cgcaggctga aaaccccgct cgtgttgatg
    24481 gatacgccct accgcttgga aaggacgctc caggatatcc agaacgcctt tggcaaaggg
    24541 cggatgatta cgataggatt gaacctgacc ttgcccacgg aaaaggtcat gcaccaagcg
    24601 gtgggagagg tgctgaagaa gttgaaaggg caaaaagcag aattcatcgt gatcgttcat
    24661 tagataattg gtatgaaatt tgtggcggaa agaggtgcag aattggtatt gctttttcct
    24721 atccttaatt cagatgcatg aacgggatgc gataattcaa ccgccccggc ttctcagaga
    24781 ttgaaaaggc tgctgaaaga cacctgtcgc aaaacagaag aattctatgg atgatgcgca
    24841 taaagcgctg ccggtctgca ccgtccgagg tggcatttca gattacatgc aatccatttc
    24901 tctaacgaat gtagaaacct ccctgctttt ttttcgtaaa ctgtagcaga aaggaaaatt
    24961 atggattcaa gacgacccgg ttgtttatct ggcataatga aattgttttt tcttgaccgc
    25021 atctatgatt ggtcacagcg gcgctttggc tataagagcg gaggttgcat gggttgcggt
    25081 tgtggaacga tattgttcat tattttcgtg tatgttctta tcagaatcat ctttggcaca
    25141 gattggctgc gcatcggctt ttaatcgaaa ggagtagaaa atgagaaaaa cattaaaggt
    25201 attgatgggg atttttgcgc tgagcatctt ggtggcttgc ggtttgcccc ttcggaacac
    25261 caatattcag ggatccggca gggtcgtcac tgatgaccgc acggtctcaa atttcaacag
    25321 catatccctc gaagggatcg gagatctgga gattgtccag ggtgatgtgg aatcgctcac
    25381 gatcgaagct gaggataata tcatcgggaa gatcgaaagt tacgtctccg gttctaccct
    25441 tcatatcagc tttgaacgtt tcatcaacat cattccaacc agtggcatta cgtacacgct
    25501 cactgttaag gatctcagca gagtagaggt ttcaggatat ggcgatgtgt atatggcttc
    25561 gttgacaacc gaccgcctgg aagtggaggt cagtggtggt ggaagggtca agattgaaaa
    25621 tctgacggcc gcttcactcg atgtggagtt gagcggggcg ggtgattttg atatagccgg
    25681 cgttgtggag gagcaggaaa ttaccttgag cggtgcgggc agctacaagg ctgctgactt
    25741 gcagagcaag attgtcgaaa ttgatatttc gggagcgggt agtgcgcagg tttgggttac
    25801 ggatgaacta gatgtaaacc tcagcggggt cggcagcttg caatattatg gcgatgccgt
    25861 ggtcagacag aatatcagcg gcatcggaaa agtggtctct ctgggagccc atgaataggg
    25921 cagatcaggt gaaaaagcca atccattcac cggattggct ttttttgtta ttcgggatac
    25981 gctgccagac cgatggtgcc tggaccaaaa tggacagcca gcgagatgga tatctcggca
    26041 aaagccactt cgacataatt gaaacgtttt tttacttcct caaccacagc ttttccggtg
    26101 atggggtcgt gggcatggac cacggcgata tacgcgcgct ggtcgccaat cttttctttc
    26161 aatcgcgtga gtatcctttc gagcgaggct ttccttgagc gcaccttgtc tttgatttca
    26221 atggtcccat cccgcagttc gatgatcggt ttgatgtcta gaatggaagc cagggttgcc
    26281 tgcaaggatt tcactctgcc actgagcttc gcgaatttca tggtgtctac cgtgaagatc
    26341 agctccattt ttcggcggat ggactccagt ttttcaagga tctcattcac ggactttcct
    26401 gccgcgtcca tctggcgggc ttccttgcac atcatcccca tgcagatggt tcctgatgcc
    26461 gagtcgaagg ggatgatatg atagcttcct ttcaattcct cggctgccag gcgggcggaa
    26521 ttcatggttc ccgatagttt atcggtcacg tggattgata agatgctgtc accgggttgg
    26581 gcgattttct tgtagaattc cacgaattga tagggggtgg gttgagaggt ggatgggatg
    26641 ctgttatctt gttcaaccat tgcatagaaa tcatcgtagc gaatatcaat cccttgttgg
    26701 taggaaacac cattgtggat gatgttgatc ggaagcaggt tgatttgata tttttcctgc
    26761 cagccggtgc acaagtcgcc tgcggtgtcc attacgatct ttaacatgat catccctcga
    26821 tatttttata ctataacccc actttttatc agaaagatat gctgtgccag cgagtgatga
    26881 cttttctcac cagttttcat caatttattg gctaaagtaa tcgcaaaaaa cgcggttttc
    26941 atgagccaaa agagattcca atcatcccct tttgttgccg tacagtgcga aacagcgccg
    27001 tcattcctcg atctttacat agatgcgttt gttgatcttg cctttgctca tgtaatcgct
    27061 gatgcggatc ttgcctacct cgtttgtgtt ggcgacgtgc gttccgccat ctgcttgcag
    27121 atccaagcca acgatctcca ccgttctgat ctcgctgata tgttccggca gcaggttgat
    27181 cttggtgcgg atcagatcgg gtatttggaa cgcttcggcg cgcggcaata tcttcaccct
    27241 gatgggatgg gcaagctgcg cttctctatt gaccgcctct tcaatgaccg ccaccaattc
    27301 ttttgtcatc gattcgaact cgaaatccat gcgcgcttcc agaggttgca tatcccctcc
    27361 ggtgaccagg gcgccgtaat ctctgaagat gacgccacac aggatgtgca tggcggtgtg
    27421 cgtgcgcatc aggcgatagc gcttttccca atcgatcgtt ccgtgcaccg ggctgccgat
    27481 ggcaggcagt tttgtgcctg cgtccagtaa atgccagtgc tcgccgttca ccatgcgcgc
    27541 ccgcgtgaac gccagatcgt cattgtcaga ggtgagcgag ccgttatccc agggttgccc
    27601 gccacctccg ggatagaagc aggtttgatc caattggatg gcgtgcagtt cttcatggat
    27661 tgcggtaatt tgtgcgtcga attccttttg gtaactatcc aattgataaa ggagtgctgt
    27721 catctaaacc tcctgcgctg tttcttggtg cgccgtgttt gaactgggga tgaaaaagac
    27781 caaaacggtt ccaaccaaac cgatggatcc gctgatgata aaagctgggt gggcgccgag
    27841 cacatcgtag aggtatccga tgatcatgct ggcgatggct ccgatggtaa acaggaatgc
    27901 ctgatagatg ccggttgaga aagagcgatt ttcgggtgaa tattcctgaa ccatagccag
    27961 cgccactggc aggtacatgc cggaaaagag cccggtaaga aataatgtga cgatctgcca
    28021 gacctggtgg gcgctgtaaa gaaagagaaa aaagaagacg gatgagccaa tcagagaggc
    28081 gaaaaggacc ggtcttccgc cgattttatc tttgtatatc cctcccatga ttgctcccaa
    28141 caccccggca ccggtggtca aggagagggc ggctcctgcc agccataggt ttactcccat
    28201 ttccgtcaga taagtgggta tgtaaacggt ggtggtcgcc tggatcaacg atcgggcaca
    28261 aacaatagcg atgatgggca gcagcatggg tgtgattgcc ttcagggagg atttcgcgct
    28321 gccattgctc actttcacat gggtttttat ggcttcgatc ttctttaatc gaaaatacag
    28381 cagcacggac acgatgaagg tgggaatgat caggtagaaa atcctgttga gggaaaactt
    28441 gttaatatac gcaatgatca agattgggct gagcatccaa cccgcttccc cgcacgccat
    28501 ccataaactc aatcctctgc cggtgtgctt tccagatact tttccggcga ttggtgatgc
    28561 cacagcgtga aaaatggatg agcttaatcc ggaaatgaac agggatagtg caagaatggc
    28621 agtgctgggt gctgagccga ccaggctcat gaaaaccccc gtggttaaag gcagaaggaa
    28681 aatatatttt tgcagattga ccctatcggc taatctgcca aagaaaggct ggctgacaga
    28741 gggccattgc agacaggcgt tcaataagcc cgcttcgaac ttggtaagcc caaagttcag
    28801 gatgagaata ggcagtagag gcgagagcat gccgccaaaa aaatcattga gcatgtgcgc
    28861 tgatgatagg atggctgtct gccatagggg atgatttctg attgctgtgc gtgtcttgat
    28921 gatatcgatc ttttgcatgg ttttttctga tgaaaattaa aagttgctaa agcatatcat
    28981 cattctgctt ttttcaatag gatttgttct gaagtcgtac cgttgagaga tttttctaat
    29041 tgcttttgcc gttacttgac agcgaagtaa tttgtggcta gaatgtagtt atcctataag
    29101 aaacaggagg tagagaaagt tcatgagcgg ttttgagttt aaaagtgatt caaaagacac
    29161 acctcctgtg ttgatttctt aagatcattc ctttttttga aaatcgaaca cagccacagg
    29221 tgtgatccta tcctgtggtt ttttatttcc ctgtggaatt cagccataag aataggggct
    29281 tgtttttgtg gtttcatgct gttcaggttc gttcaagtag attggaaatg ggaaggttga
    29341 aagcgtgggc gctgtttatc gcgtaaagaa aagccgttgc aggctacacg tcgtttttgg
    29401 aattatgaaa caaggagaat tgcattgcta aagaacatca cgaatctata tcgtaaattc
    29461 aacaaagatt tctggattct ggttggagca tctttcattg atagtgtggg aggggcgttg
    29521 atctttccgt tcctcaccct ttacatgacc cagaaattca atgtgggcat gacagaagtt
    29581 ggtaccatgt tcctgatctg gggtttggga gcgggcttgt tgggcaattc gcttggtggg
    29641 gcgatcgccg ataaattcgg gcgcaagaag agcatcatct tcggattgat cgccagtgcc
    29701 agcagcgcgc ttgccatgat ctttatcaaa gacatcgtgg ttttctatat tgtgatcggt
    29761 gtcattggta tctttgaaga tatcgccggt cccgccaggc aggctatgat cgctgacctg
    29821 gttcctgaag aactgcgcac cgacgcgtat ggcatcatgc gcatcgaatt caacctggct
    29881 gtgactatcg gtccggcgat cggtggaatc atggctgcca ggtctttctc aattttgttc
    29941 atcatggatg ttttcatcag cttggcggtg gcgggcattg tgctgctttt cctgcccgaa
    30001 acaaaaccgc agatgcaaga acatcaaaga gaagaaagtt tgaaagagac tttccagggt
    30061 taccgcaaag tgttcaaaga taaattgttc atcgcctttg tggtagtgat gatgcttgag
    30121 accctgatgt atttcaacat gaacagcact ctttcagtct atctggtgaa cttccagggg
    30181 atcaccaccg agcagtttgg ttatgttctc tccctgaacg cggtcatggt tgttgtgcta
    30241 caggtctttt tctctcgttg ggttgcgaat tggaaaccca tggtcaccat ggcgcttgga
    30301 aatatcttat acgtgattgg cttttctatg tatggttatg tgaattccta tcctttgttt
    30361 ttgctagcca tggtgatcat cacgatcggc gaaatgatca ttgcgccggt cgcccagtcg
    30421 ttggtggctc attttgctcc tgaagatatg cgtgggcgct acatggcggt gcattccctt
    30481 gcctggatct tgcctgtttc ggtcggtccg ctgggagcgg gctacatcat ggataatttt
    30541 gacccgcgtc tgctgtggtt cgttgcgggc ggaatcggct tgttggacgt ccttggcttc
    30601 ttgttgttgc atttcaaagc cggcaagaaa tttgaggaaa ggcagaacgg gtacaaggat
    30661 aaaagcgaag acccatcagt gcaggtggaa accacaccca tgatcgccga ttgatcctgc
    30721 ttgagcggga ggcggggttt gaagacgacg tctcaaaatc gatgcacgcg aacttctgtg
    30781 aacagagcgc gtgccccagg gcgtgcatgg tttccttaaa ataagctgcc gcttgttgat
    30841 ttccctggga tccaacccgc cgtgtggcaa gcgtttcgca caggaactgc aaatgttctt
    30901 gtgctttttc taccagcatc tcttttggca tggcatccat ttcatttcaa gataaaaatt
    30961 gattctagca cactggtcgc aatgaacgtt cccccctctt tttgagcaaa ccagattggt
    31021 tcattcttct ccgaaattgt tctcgatcag gagcttcagg tcttgcagcg ctgattgctc
    31081 atcttcccct tcggcgaaga ttgtgatgtg agcgtctttt tctatcctta accccagcaa
    31141 tcgaatgaga tttttcccat ccgcttcttt attgttatgc atgacccgga cggtggcttt
    31201 atatttacac gcagtctgga caaattgaat agcagcgtgc gcgtgaatcc ctagttcgtt
    31261 gatgattgat gcttcgattt tcatgaccaa tgaaaaatat aaaaatttca aacgagagca
    31321 ttatagctta aataaagact gagcatggag caaaaaggat atctcttgga gaagcgttgt
    31381 gcttttaaac gcatcaaaag ggttctattt tcgaattcag catatcatca actgcctcct
    31441 caccatcgat gccggcaatg atctcgcaag caacttttcg ccgaatttca ttgggatctc
    31501 ttgtttgttt ttgaaaagct gaaatcattt tatttttttc gtccggagac cacctggtca
    31561 ataagcgaat gtacgatggc aatgctttag gaggaagctg gaaaagcttt ctgtactttt
    31621 ctatgggcgt ttcgttgatg ccaatatagt tgccggtgct ttttgacatt cttttttccc
    31681 catcaatgcc gaccaggatg gggaaggtta aacatatttg tggtggcaag ccgaaatatt
    31741 cctgcaattt tcgtccggtc atgaggttaa ataactgttc cgttgcccca agctggacgt
    31801 ctgcgcgcaa ctccacggcg tcatatgctt gcaccacagg atataagatt tcttgaaagc
    31861 gaaccgtgtc acctttttcg atcctttttc tgaaattctc tctggataac aattgctgaa
    31921 aggaaaattg cgctgcgatt ggaatgaaat cggatacttg cagatcgttc aaccattcat
    31981 gattgaatct cagccttgtt ttctgtgcgt cgagaatttt aaacgcttgc tgtgcatatc
    32041 gctttgactc ttccataata agcgcaacgc tgcgcggttg cctggctgtt tcccgttcgc
    32101 tggggtctcc aatgagtgag gtgaatgtgc cgacgaccaa tgtgacatca tgcccccggt
    32161 cttgaaactg gtggagtttt cgcaaagtaa cggtgtgccc aaggtggaga tcaggcccgc
    32221 taacgtcgta tccgcaataa actcgcaatg ggcgtttctc caatgctgtt tgttgtagtc
    32281 tttcttccaa ttcgtggcgc atgcgcgctt ccaggttctt atccccgaac tctgaaccgt
    32341 ccagaaatgc tgccagttga ctttgaagtg aaagatttcc catgctgccc ctttatcata
    32401 ttatagacgc ggatcaataa gaaaaaaggg tcgcgcgatg tgaagtgcta tggattgctt
    32461 aaaataaaaa agcccgcttt gggcttgtgc ccccatgggg attcgaaccc cagttttagc
    32521 cttgaagggg ctgtgtccta ggcctctaga cgatgggggc agagagggtt attttagcat
    32581 ttgattctgc acaggtcaag caattggtat gtctgaattt tttcgctgtt gaaaaaatgt
    32641 aagaatactg taagatttca attctgtaat tgacttcaaa aggtcgctat gttaccatgg
    32701 tttcagaatt tatccgcaat atttatcatg aactttaaca aattctgaaa ggatttttca
    32761 tgaaaaaccc caaaaatctt gtacttatta tccggttttc cggactgatc ttattctctt
    32821 tgttaggtat ttatttagga aatctgattg ccaaaagttt cgacatcact gtcatcacaa
    32881 ataattatgc catgatcatc ggcggcgtcg tattgggcgc cctcggtttt tttgcgttcc
    32941 cctatatgac catttatccg gttcaaacca ttcgcaagag tttggaggaa acccccggtc
    33001 aaaccctcct ggtcgctatg gctgggctga tcgttggctt gatcattgct gctctgatct
    33061 ccatccccat cggtgttttg cccgcgccgt tgaacagcat cctgatcatt tttgcgctgg
    33121 tcttgttttc ttacctgggg gttacgctca gcgtatccaa gcaggaagag attcgtgagc
    33181 ttttgtcgtt tgctcgtaga gaaaagaagg ttggctcttc gtccaagaaa actggcggag
    33241 cgggttggaa caaagattgt tccatcctgg tggataccag cgtgatcatc gatgggcgta
    33301 ttgccgatat cgccaagacc ggctttttgc ccggaaccct cattatcccc cacttcgtat
    33361 tgaacgagct gcagtatatt gccgattcgg cagaaagttt acgccgccag cgcggacggc
    33421 gcggtatgga agtgctttca gaactgcaga aagatcgcaa tctcaacgtg gttctttctg
    33481 atatggatgt ggagaatgtg cgcgaagtgg atgaaaagct ggtggtgctg gcgcgtcaga
    33541 tgaaagcccc catcctcacc aacgattaca atttgaaccg tgtggcagag ttgcagggtg
    33601 tgcaggtgtt gaatatcaat gaattagcca atgcggtgaa atcgatattg ctgcctggtg
    33661 aagaattgac catcaagatc attcaagagg ggaaggaaca ggatcagggt gtggcttacc
    33721 tggatgatgg caccatggtg gtggtggaag atgggcgcgg atacctcgac caggaaattg
    33781 tggtgaccgt gacgaaagtc ttgcagaccg ctgctggtcg tatgatcttt tccaagccgg
    33841 gcggtgctaa cggaatagac aaggagtaac agcagcatgg gcttgcgtgt gtataacact
    33901 ctcacacgag aaaaagaagc gtttcgcacc attcaggatc gcaaaattag catgtatgtg
    33961 tgcggtccaa cggtgtatgc caacgcgcat gtcgggcatg ctatgtctgc gcttgtcttc
    34021 gatatcatcc ggcgctacct ggaatatcgc gggtacgaag tacgccatgt catgaactat
    34081 accgatgtgg atgacaaaat catccagcgg gctgctgcgc tcaacaaaga tcctcgcgcg
    34141 ctggcgcagg ggtacattga caaattcgac gagcaccttc gcgacctgaa tgttcttcct
    34201 gccacctata agccgcgcgc caccgctgaa atgccgaaca tcatcaagtt cgtggagaac
    34261 ctgatcgaaa agggatatgc ctacgtggtg aacggcgatg tgtattaccg ggtgcgcaag
    34321 ttcaaccgct atggaaagct ctctcatcgc aacgtggatg agatgcgctc tggcgcgcgc
    34381 atcagtgtgg atgagcacaa agaagatccc ctggattttg ccctgtggaa gagcgccaaa
    34441 cccggcgaac ccagttggga aagcccctgg ggtcctgggc gcccgggctg gcatattgaa
    34501 tgttctgcca tgagcatggc gcatctggga gagcagatcg atattcatgg cggcggcaat
    34561 gacctggttt ttccacatca tgagaacgag atcgctcaat ctgaagcgct tacggggcag
    34621 ccctttgcta cctactggat gcacaacggc atgctccaat ttagcggcga aaaaatgtcg
    34681 aaatctttgg gcaacttggt tacgatcgaa gatttctgca aagaccatcc ggcggatgtg
    34741 ctgcggatca tggtgctcaa ttcgggctat cgcaatcccc tgaccttcaa tgacgaggtt
    34801 atcgaccaag cccaaaaagg tcttgagcga ttgcaatcgg ctttgaaaga cgcgttgccc
    34861 ggtgcggaag gcgctccgca agccagcctg gaagcgctgg cagcgcagat aacagccaca
    34921 cagcagggtt ttgaggtctg catggatgac gatttcaatt ccgccggcgc gctggggaac
    34981 ctgtttgatc tggtgcgcgt catcaaccag gtgcgcgctg atggcgctac cgacgaacaa
    35041 ctgcaagctg cccaggcagt gttgtctgaa ttgagcggtg tgctaggatt acgtttgcag
    35101 gcaacccggc aggagcatgc cgcggatgcc ttcattgata tgctggtagc attgcgcaat
    35161 gaattgcgta ccaaaaaaga atgggctctg tctgatcgca tccgcgatga actggcggaa
    35221 cagggtgtga ttgtggaaga ttctgccagg ggcagttcct ggcactggaa atgagagagt
    35281 ggatcaccgg tcgaaacccg gtctatgaag tgttgcgtgc caaccggcgc gagttctttc
    35341 gtttatggct ggcggaaggc aatttggaac agggtgggca cttgcaggat gcgctgacgc
    35401 tagccaggca gaaaaaaatt cccgtcgaac gcgtcccgcg cacgaaactt tttgagatat
    35461 ctgccaatca ccaggggttg gcgctgcagg ttggcggcta tcgctatgcc agcttgcctg
    35521 aaatgctggc actagccaga aatcgtgccg aagctccctt catcctcctg cttgatctca
    35581 tccaggaccc gcaaaacttg ggcaccctgc ttcgttgtgc cgagggattc ggtgtgcatg
    35641 gcgtgctcat cccttccagc cgggctgccg aagtgacccc ggctgtggtg aacgcatctt
    35701 ccggagcctg cgaacatctc ctgattgcac agatgaacct tgcacaatcg attgaggtgt
    35761 taaaagaaga aaatatctgg atctatggat tggaatattc cgaccaggct gtgcccatcg
    35821 aaagtgtgga ttttcatggt ggggtggggt tggtggtcgg ttcggaaggc agcggcttgc
    35881 gcagcctggt gcgcagcaag tgcgaccggt tgattagcct gccgcgccgc gggaaagtag
    35941 attcgctgaa cgcggctaca gccggagcga ttgcactata tgttattcaa catgccagac
    36001 agaaaaactt ataacaacca ctgaccggct tgcaccaccc aggtgatcca cagcccgatc
    36061 atgatcatgc cgatcaggaa gcgcaccagg aacgaacagc cgaatccttt caacatttca
    36121 ccggttgatt tcagcgcgat ttccgaattt ttcaaacgga tggcttccag cacaaagagc
    36181 accactccgg cagcgatcac acctccaaaa ggcggaaacg cgatgctgcc gactacggct
    36241 gcgacgacgg tagccagagt ggtcagccag ggcgcgccac ctttgcgggc gccggctccc
    36301 atcatcaggt tatcgaccac attccccaac agcataagag cgctgatgat gccgatgaag
    36361 atcccccccg gcttctcgaa gccgactacc agcccataga tcagcgccgc cagccagatg
    36421 aaggtgatgc cgggaatgcc agggatgatc agggtgatga ggctgaccag catcacaatg
    36481 gcaaccacga tctgcagggc tgccagggtt ccgacggtca gattatcctt tgctccaaaa
    36541 gtttcaatgc tgcctgtctt tccgtcagag cagccagata gggcaaggat cgtcaggacg
    36601 gaaagaaaca aaaacaaaac ggctttccat gtcggttgca tgaatatttc ctccatttgc
    36661 ttaacgatat acgcaaatta ccggataacg gttgcgccgc ccatccaggg ttgcagcact
    36721 tcgggaatgc ggatgctccc atcggcctgc tggtagtttt ccagtatggc gatcagggtg
    36781 cgcggcaaac ccaaccctga cccattcagg gtgtgcacgt aatctttgtg cccttcagcg
    36841 tctttgaatt tgatatttgc gcggcgcgcc tgaaagctgc cgacgtttga aatagaagag
    36901 acttccagcc attcctggca gccgggtgcc caaacctcta cgtcatatcc gatgcaggcg
    36961 ttgaagctga tatcgcctgt gcaaagctgc aggacgcggt aggtgagccc cagttgttgg
    37021 caggtttcct cggcatccgc tgccatgcgc tcgaattcat gcatagattc ttcgggtttg
    37081 cagtaggtgt acatctccac tttgtcgaat tgatgcccgc gcttgatacc gcgcacatcc
    37141 cgcccggcgc tcatcttttc ccgtcggaag caggggctat aggcggtgta gcgcagcggc
    37201 agcttttcgg catcgatgaa ctcatccatg tgcaagccag tgaggggcac ttcggcagtg
    37261 gggatcatgt acaggtcttc ttcgtggtcc ttgtacaggt tatcggcgaa cttgggaagc
    37321 tgcccggcgg cgaacagcgt ggtgcttttc accatgaaag gagtgtattg ctccagatat
    37381 ccctgccgga cgtgcaggtc gagcatgaag gcgatcagcg agcgttgcag acgggcgccc
    37441 agtccgctca gcacataaaa acgcgaaccg gtgatcttta ctcctcgttc aaaatccaga
    37501 atgcccagtt tctctcccaa ctcccaatgc ggtaggggtt caaaatcgaa agggagtttc
    37561 tctcctaccg tcttgatgat cacgttctcg ctctcgtctt ttccgatcgg cacctgcggc
    37621 agaggggtgt tgggaatggt catgatgagc tgttgttgtt tttcttcgat ggcgcggatg
    37681 gtctcatcca gagcggtgat gctgtcggaa accactttca tgtctgcaat cttcgcctct
    37741 cgctcgttct gatctttgat ctttccgatc tcgcgcgaaa cggtattgcg ttcggctttg
    37801 agcgtttcca ctttttgcag tagttcgcgg cgctgctcat caatggcaag cagttcatcc
    37861 accgcgctgg aatccaggtt gcgtttctgc atcccttcgc gcacggtttg tggttcttca
    37921 cgcacaagtc tgatatcaat catgatcgct ctccttggtt taatagaaaa acccctcttc
    37981 ctgcaggacg agggggcgct cgtggtgcca cctgcttttg ccggtgatga ccgacctctt
    38041 tgggcggtaa cggctgcctc cgtccttctt acgatgcgcg cgcatccctg cgaaggaagc
    38101 ccgctcatgg tgagcgaccg gttgggatga ccgggctggc ttttcctttg cccagattat
    38161 acaattccac gggaggatgt caagagaacg agagaacggt atataatctt ggcgtatgga
    38221 tgaacaaacc cttgtccagg cagcccagaa aaatgatctg gatgctttta accagttggt
    38281 gctggcgcac cagcaggttg tctataatgt tgccttgcgt atgctcaatg acctggatac
    38341 ggcggatgac gccacccagc aggcttttat ctctgcctat caaaagattc acacctttaa
    38401 aggtggctct tttcaagcct ggatcctgcg catcgtgacc aacaaatgtt acgatctgct
    38461 gcgcagcggg cagcgccatc ccacccgctc cattgatgat gcatcggacg atcctgacgt
    38521 ggattgggaa agcgctgctt tgatgcgcga tgacgctcct ttgcctgaag aacaggtggc
    38581 gcagaaggaa ttggaagccg cggtgcagca ttgcatcgat gagctgccca gagagtttaa
    38641 ggcggttgtc attttggtgg atctgcaggg catggattat caggaggcag ccgaaatcgt
    38701 ccgttccccg ctagggacca tcaagagccg tctggcgcgc gcccgcatga acctgcagga
    38761 ttgtctgcac aagttcaagg aacttttgcc cgcccaatat cgttcagaaa gtgagggcag
    38821 ccatgactga tgtgaccaga aaagaatggc ttctgctctc gcgatatttg gatggtgatc
    38881 tatcggcgga gcaggtcagg cggattgagg aaaaactgcg ttcggatgtg gctttacggc
    38941 aagcgttcga acggttgctg cgcacccgcc aggtgctgcg cgccgtgccg cagcgcaaag
    39001 ctccccgcac ctacctgctt tccaacgtga tggtgcgggt ggagcgacag cgcgtgcttc
    39061 cacagttggt atggcgctat tcctccatag ctgctgctct ggtcgcgaca atcgctttgc
    39121 tgttgcaggt cttttcaccg cgcgcatcgc aggcaagccc gctttccgca tcagcccaac
    39181 ccgaaatgat ggttttaact gcgccggatg cgcttgatgc tgaacctacg gaacaaccac
    39241 tgatcatctt ctggaatccg ggcgcatatg gcatgggcgg tggcggggag ggccccagca
    39301 catccatggc ccccgctgcg ccgcttgagc aaacggattc ctatggggtg agtggtggcg
    39361 catctactac ggaagacaca tcttctgacc agggtatgtt tgccaaggaa gcggaagaag
    39421 ttgttcctga aatggctgat gaatctgctg tcgctcagga acaggcgctg cctgaagagc
    39481 aggcgctcag cgctgccgct tccccatcgt ccaaccccat tctgggtatc gcccctcccg
    39541 agaaacgcgg cagtctgcaa gagttcagcg ctgaagaaaa tttgcaaccc cgggactcgg
    39601 tcggggaggg ttcagcgctt aattttggcg tgattgccct gctggcgctc tttgtttcaa
    39661 tcgtttgcgg tttggtggca tttatcttgc accgaaaaca acagaaacag taacccacgc
    39721 tgtgctatac ttagaacaag atttcggtta attgaaggtg gtgttttggt ccaaaaaaga
    39781 agatccagcg cgtcaaaaag ccgttcgacc gctaaaaacg gtaaaaaggt ttcccgcaga
    39841 tccgcacccc tccgcaaagg gttgaaagac ctctcgccag agcgcaagtt agatatctgg
    39901 ggtatcggtt taacggtgct ggggctgttg ttattgctca gttttgtcac cagccaggag
    39961 ggcaacgtga ccggcgcgat tgtgctgttc ctctaccgca ttgccggtta tggggctttc
    40021 ctttttcccc tggtatgtct gctgattggg gtttggctta tcttccgcaa cgaagaccgc
    40081 ctgcccatga tctccagcgg taagctggta ggtatcatca tgctgttctt caatttgctc
    40141 acctggatcc atttgttgac cggcgggggg tgggatctgg cgaaagccgg cgggggaggc
    40201 ggatatatcg gcgcgttctt tgagaacctc atcgttcgtg cggtgggaca ggtgggtgcc
    40261 ttcgtggtct tgctcggctc gctgctgatg gctgtagcat tgaccattga tacagccatc
    40321 ccggatctgc tgcgtggcgt caaaggtgca cttgccaaaa ccggcaagca ggtagctgcg
    40381 cagaccaccc agcttactca aaaagtggct gcacccaaga cctctacccc gaaaaccgaa
    40441 cggcggaaag cggctgaaag ctcttccatc gatctggagg gtttcacccc catttccgaa
    40501 gcgcaacatg cccgaccctc tgccacgaag caaaaggggc gggctgagcg cgaacagcca
    40561 cccccagctc gcccgcgaac gcaacctgcc cccaaggatg ctgctaccgt gcagcttggt
    40621 ttgcgcgcac aggttgccag cccagtctgg aaattgcccg ccatggcgga tattctcaat
    40681 cttgctaata agaatgaaaa cgatggtgcc attgacaagg agcgcgctcg catcatcgaa
    40741 gagacgctgg cttcttttga tgctccagga cgtgtggtgg aaatccaccg cggacccacc
    40801 tttacccagt atggggtgga acccgatctg gtgcaaacgc gcaacggtcc cacccgcgtt
    40861 cgggtgagta agatcaacgc tctgtctgat gacctggcgc tggcgctggc aacccagcgc
    40921 atccgcatcc aggctcctgt tcccggcaag cactacatcg gtattgaggt gccgaatagc
    40981 gaag
//
LOCUS       DAOSDS010000010        39913 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_547_length_39913_cov_5.679136, whole genome shotgun sequence.
ACCESSION   DAOSDS010000010 DAOSDS010000000
VERSION     DAOSDS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 39913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39913
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_547_length_39913_cov_5.679136"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1178)
                     /gene="ulaG"
                     /locus_tag="PLL88_02365"
     CDS             complement(75..1178)
                     /gene="ulaG"
                     /locus_tag="PLL88_02365"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HOJ00437.1"
                     /translation="MSKVSEMTREKWIMSTFPEWGTWLNEEIENEVVAPNTVAMWWLG
                     CTGIWMKTPAGANISIDLWCGNGKRTHGDGKMKVGHQMANMCGARMMQPNLRAIPFVI
                     DPFAVKQLDAVLATHYHQDHMSVEYAAHVLQSNLTTVDAAGKQIPVPFIGPKKSVETW
                     VKWGVPEDRCITVKPGDFIKVKDIEIVVLDSFDRTCLVTTDSTDADREELTNVCPTDM
                     DEKAVNYLLKTPGGNIYHSGDSHYSIYYAKHGKDHKIDVAFGSYGENPVGMADKMTSI
                     DILRMAEALRCKVVIPIHYDVWTNFMADVNEIKVLYEMKKDHLGYQFHPFFWEVGGKY
                     IYPTDKDKRAYHHRRGFEDCFEAPQNIPFRSML"
     gene            complement(1542..2252)
                     /locus_tag="PLL88_02370"
     CDS             complement(1542..2252)
                     /locus_tag="PLL88_02370"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOJ00438.1"
                     /translation="MKRKILLSTLVGALFLAFLSACSVGVERTETREINGVSAISINT
                     VGKFIIRQGDEESLTIQGPNNILRNISTEVVGDTLYINSTRGVLGDSLGRTVYTLTVK
                     DLNELSLSGAGSISIASLDTDHLEITLTGAGSIDVTALNTRQLTVLLNSAGAIDISGT
                     ALNQNVIISGVGSYHAPNLQSSNASVVMSGAGSAEVWVTDVLDIEVSGVGSVGYYGSP
                     SVTQNISGLGSVDSRGEK"
     gene            2320..2673
                     /locus_tag="PLL88_02375"
     CDS             2320..2673
                     /locus_tag="PLL88_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00439.1"
                     /translation="MRPTRGYYRGLPQEPQKLFSGGLSAPHALQTTWERLFFFHFFFH
                     GLEVSIVMALVFFAIQIKKISEKMINPIATIMEIITGLDNMVVILYSKFWLGGGTETP
                     CAALQEKLTARTEVL"
     gene            2746..3513
                     /locus_tag="PLL88_02380"
     CDS             2746..3513
                     /locus_tag="PLL88_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ00440.1"
                     /translation="MLSDERRAVLINRLREDGYVQAAEIAEELSVSTATIRRDLVFLQ
                     QEGFCMRTRGGAVRSSQGTTLELPYALKKQKFVAEKEAIAREAVRLIENGDTLILDSG
                     STTYALASLLTEKQRLTVVTNDLQIAILLASNPSVHLVCTGGIARPHVFTLQGSEVVN
                     FIKTLRVDKTFLGTDAIHEDGTIGNVNMEEVAIKQAMMKAASTVILLADSSKFTVKGF
                     AQVGTLAEVDILITDAKYPKEMATVIQENAIKLVVAK"
     gene            3522..4166
                     /locus_tag="PLL88_02385"
     CDS             3522..4166
                     /locus_tag="PLL88_02385"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOJ00441.1"
                     /translation="MIKLSPSLASASFLDLQNTVRQLEKAGADYLHFDVEDGCFVKEM
                     RLGIKVIQDLRAITDLPFDVHLMMVDPEWIVPVLAKMGVELLSVHFEATEYPRRTLGI
                     IQDAGMKAGLAFNPKTAIPDLSFYFPYLHFVNVLTTEPEVKNCAYLPQVLTKVRQVKS
                     AYAAQGVLCEVDGGFSPENVLDAIHAGADIIVAGRGIFGDSEIENNLRAMKSAG"
     gene            complement(4242..5060)
                     /locus_tag="PLL88_02390"
     CDS             complement(4242..5060)
                     /locus_tag="PLL88_02390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HOJ00442.1"
                     /translation="MKPYWTEEVFRTNKPIIAMCHLQALPGDPAYDRVAGMKKVIALA
                     RADLLALQEGGVDGVMFSNEFSLPYLLKVNPETIAAMGRVIGELMHEITVPHGVNVLW
                     DPYASLDLAAAVDAKFVREIFSGVYASDFGLWNTNCGTVVRHQHALGAENVKLLFNIV
                     PEAAQYLTPRDIGSIARSTVFNHLPDALCVSGLTAGVQTDAATLKLVKSAVPNTVVFA
                     NTGMRLENVEEQLSIADGAVVATTFKVDGIFQNAVDVQRVKTFMDKVKDFRRNF"
     gene            5198..5839
                     /locus_tag="PLL88_02395"
     CDS             5198..5839
                     /locus_tag="PLL88_02395"
                     /inference="COORDINATES: protein motif:HMM:NF005123.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOJ00443.1"
                     /translation="MLEALKKEMVELLKELPQNGLVCLTSGNVSARDAKTGLVMIKPS
                     GVPYEQLDESKLVVLNLDGKVVEGSYKPSSDTASHLYIYQNRQDVNGIVHTHSAYATV
                     FAALGKNIPVLTTETAEEFGGEIPCAEFVLIGDDEIGKQVVLYGERCNAVLLKKHGVF
                     TFAATPRRAVDLAILTENSARIAWLILQMGEPDGIGAEEIKALYYRQQNIYGQ"
     gene            5943..7301
                     /locus_tag="PLL88_02400"
     CDS             5943..7301
                     /locus_tag="PLL88_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ00444.1"
                     /translation="MDLFDYQLSVNLSNQAPLAARMRPRTFAEFFGQEHIVGEGKMLQ
                     RAVLADRLFSSIILWGPPGTGKTTLAQIIANYSKSYFVSISAVMAGKAELKSVIDEAL
                     ERRRLYNKRTILFVDEVHRWNRAQQDALLPNIENGLIILIGATTQNPYFDVIPALVSR
                     SRVFSLKPLDEKALAAIFENTLKDKERGYGNIPIDIDDDAREHLIQICGGDARNLLNA
                     LELAVESTPRGEDGRLHISLQDAQESIQKRAVRYDRAADEHYDTISAFIKSVRGSDPD
                     AALYWLAKMLYAGEDPRFILRRLLILAGEDIGLADPQALQVAASATYAFEYVGLPEGV
                     FPIVEATLYLATAPKSNSALGYFTALKEIEEKGAQAVPIHLMDGNRDSKALGHGKGYR
                     YPHEFPDHFVAQQYLPTDLIGKTFYEPSDQGREKAIAEALARWREMQDRVLKDQFSNQ
                     AP"
     gene            complement(7306..7890)
                     /gene="rdgB"
                     /locus_tag="PLL88_02405"
     CDS             complement(7306..7890)
                     /gene="rdgB"
                     /locus_tag="PLL88_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOJ00445.1"
                     /translation="MQLLIATTNLGKIKEIKSILSNIPAHLVTPAEIGLNLDVDETGS
                     SYRENAEIKALAFSRASQLPVLSDDTGLEVAALSGLPGIHSARFVNSPHASDGDRRKL
                     LLEKLSAYPRPWKARFICVATLVIPGGSPISMEGECVGEIIPEERGEYGFGYDPLFLV
                     QGTRKTMAELTLPEKNSISHRAKAVQALIPFLQE"
     gene            7993..8463
                     /locus_tag="PLL88_02410"
     CDS             7993..8463
                     /locus_tag="PLL88_02410"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOJ00446.1"
                     /translation="MKQPRRPFRINVGFLINQPIGFSREIPFSFESYQFPNDEKMEDL
                     CGTIVLIRTQTGIQALMDFSAVVDAQCVRCLEDFKQALHTSFQELYTYSLEPLSEDEK
                     SIPEDGYLDFEESISDYILLEFPIKPLCKADCRGLCMVCGQNLNLSSCEHVAQA"
     gene            8519..9697
                     /locus_tag="PLL88_02415"
     CDS             8519..9697
                     /locus_tag="PLL88_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ00447.1"
                     /translation="MSYDEQKIKDDILEILLEQSETHDELSMEDVYNALPDNEEFDQE
                     FIYGLVGQLRKQGVEVAEEEGEPLADEAEIPLTDMDPYVGLSHIASDDTISLYLREMS
                     RVPLLDSNEERNLAIRIENGEAAQIELSQLNGNATPQQKEELERIIASGKEARAHLIK
                     ANTRLVVSIAKRYTNRGVPFLDLIQEGNLGLMKAVEKFDYTKGFRFSTYATWWIRQTI
                     TRAIADQGRTIRVPVHMVDRIRQMYKQIHELEQTLGRTPTIDELSKALGTDKQKVSWM
                     LRVSWLPLSLESPINEDEDESELGHFVEDKETPTPIQTVYSKLLKEKVNDVLDTLPCR
                     EARILKLRFGLEDGKYYTLEEVGKKFGLTRERIRQLETKALRRLRQPQKSQQLRDYRQ
                     "
     gene            complement(9701..10459)
                     /locus_tag="PLL88_02420"
     CDS             complement(9701..10459)
                     /locus_tag="PLL88_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ00448.1"
                     /translation="MHFSNGYAYQEFHADRSALLPTVLIHGAGSSHLGWPAAIRRLPG
                     MHILTIDLPNHGAAEKKDLHSIEEYAVDLHAFLLGLEMKRVNLVGYSMGSAIALQLLL
                     RFPAICCRAALLAYAPQPSFNEISAPHGPQNELSVDWQQVFMDKLLAGGFSNSQKEKL
                     LQPFLVEDAATLKHDLWITHTYHPRFPDEKLPTPVMFLNGAADPFMNVEEKQSMLASF
                     AEPVIKEIHNAGHLFLWEYPELVRLYLADFFSQN"
     gene            complement(10471..12096)
                     /locus_tag="PLL88_02425"
     CDS             complement(10471..12096)
                     /locus_tag="PLL88_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HOJ00449.1"
                     /translation="MKKTKEAWRTAKQYTPEERAIARLVLDEVKAGAPLMKAIHAHPL
                     KAGGYIAKHALIAQYQQLVQTGTYPDDPVFLSAIRMKPIRSLSGVTTVTVLTKPFPCP
                     GDCIFCPDDQELPKSYLREEPGAARAFENQFDPFLQVSSRLASYDAIGHPTSKIELLI
                     LGGCWTAYPADYQTWFIQRCLDAMNGVDSTSLEEAQAMNVAGERRNVGLVIETRPDAI
                     DAAVLARLRQLGVTKVQIGAQSFDDRILAMNRRGHTAADTLQAIALLRAAGFKIVMHW
                     MPNLMGATIASDRDDFKKMWEGGYCPDEIKIYPTQLVENSQLYQYWQRDAYKPYTTQE
                     LVQLIADVKLSIPEYCRVNRVIRDIPATYIVDGNKRSSLRQDVQNELKARGERCRCVR
                     CREIRGKLVDEETLMLDEVVYHPACSEEHFLHYRTPDDHLAGYLRLSFPDSTHEQGQQ
                     VYQQVYQHIPELRGCAIIREVHIYGQSLRFGTENTGAAQHVGLGSRLIEKAVELCRER
                     GIQQLAVIAAIGTRHYYFNRGFTGGKLYMLRRV"
     gene            12126..12890
                     /locus_tag="PLL88_02430"
     CDS             12126..12890
                     /locus_tag="PLL88_02430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ00450.1"
                     /translation="MVDELTIQRIHALNREFYQTFSGSFSSSRYQVQAGVRRIVRTFP
                     TSGSILDLGCGNGNILRELAAVGFQGTYHGIDFSEALVNHARNFCEAMQPGAAFRAQF
                     ETHDLISPSWKNSLTAQQYERICAFAVLHHIAGGEKRKAVFQAIRSLMSPVTEFHFSV
                     WQPQNSKRLLKRILPWDTVDIDEKKLEAGDILLDWLAEEESRHRTGYRYVHVFSIEEL
                     AEIAKTLGFRVVGSFFSDGESNNLGLYQHWRATALP"
     gene            12975..13610
                     /locus_tag="PLL88_02435"
     CDS             12975..13610
                     /locus_tag="PLL88_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00451.1"
                     /translation="MAGQEVDLLKIFNQVSKTLKTNQDALNQADEYNHDHGDHMVEIF
                     EVITSAMKTKKNADPADQLDYAAQLLRQKSSSGSATTYANGLTQASKQISGKSINIDT
                     VLPLLQTLLSGGETGASSSGGLLGDLLGTFLGGASSTTTSGGSGGIDLSDLLGAGLSY
                     MQSKQAGSSDMEALSKVLLGGSQYSSNPYRTQSGELIANTVLQALSGLMSK"
     gene            13704..>14618
                     /locus_tag="PLL88_02440"
     CDS             13704..>14618
                     /locus_tag="PLL88_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ00452.1"
                     /translation="MPYLFIALGIVLLVFLFMVLRTLQLSGSPHQPSSADLMQVEVGK
                     PAEHLAEALRFKTISKLKMEAADSEPFKKYHLWLEKTYPKTHTILKKEIVNQLSLLYC
                     WKGSESRLQPVLFASHLDVVPVDEATLPDWHADPFKGEIKQGFVWGRGSLDMKNHTIA
                     LFEAVEGLIVAGFQPKRDIYFAFGHDEEISGKDGAKSMAALLKRKGIRLAAVLDEGGM
                     ISHGLVPGVETPVGLIGIGEKEYVTVNLIAKGTPGHSSNPPRQTAIGVLARAIALMDD
                     HPFPARPALAIATLRGIAYLFPFAVRFAL"
     assembly_gap    14619..14628
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14629..15173
                     /locus_tag="PLL88_02445"
     CDS             <14629..15173
                     /locus_tag="PLL88_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ00453.1"
                     /translation="WLLKRALVKQLEQKVQTNALIRTTHAATVFNGGIKDNVLPAEAR
                     AKVNFRLLPGDTQDTVLQHITKVVDDPRVQTEIDLADAWGPCKVSAIDSPAYKTLELV
                     IRQVFGEMPVAPYLLMFATDARHYQDVSEQLYHFSPLAMESDDIARMHGIDERISVEA
                     LSGMVKFYARLMKVWGEAEF"
     gene            complement(15178..16191)
                     /locus_tag="PLL88_02450"
     CDS             complement(15178..16191)
                     /locus_tag="PLL88_02450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ00454.1"
                     /translation="MKIKVCNSFDDIEQSEWDALLQKSSINVPFLRYGFLKLWWQTLG
                     GGEWQVGEPYIMLGYQQENLVAIAPLFLSHRDGKNILTFLGAKEISDYLDLIVPSDYF
                     HDFISAVLHHLLQDIPEKWDRVEFSNVHQSSPLLQFFQNEAMVDGIACHVEKDSPAPC
                     LTLPVSWDAYLESLDKKQRHEIRRKLRRVEQEAQQHRFFFVDDQNDLEKMALAFLDLM
                     RKDPAKNAFLTTSMAAHMQRLLSWSYAEGILKLCFLEINGVLASGFFCFDDQNTIYIY
                     NSGFDSRLQYFSPGWVLLSHLIRWSIENSRAALDFMRGNEAYKYKFGALDALVYSITL
                     TKR"
     gene            16257..16922
                     /locus_tag="PLL88_02455"
     CDS             16257..16922
                     /locus_tag="PLL88_02455"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOJ00455.1"
                     /translation="MQQPQILLASSSPRRRELMAWMKMPFTATSADIDESRRKDENAL
                     EHVRRLAREKAEKVFEEKHREAVILAADTIVIKNDHVLGKPQTERQAVEMLVELRKTT
                     HQVVTAICIIQPDGIRREDVCISEVLMRDYSDEEIQRYVASGDPMDKAGAYAIQNGTF
                     NPAPEFKGCFASVMGFPFCHIERNFRKIDGYNWIPVTAICQQNLRYVCPIFAKVLNGE
                     NIG"
     gene            16927..19152
                     /locus_tag="PLL88_02460"
     CDS             16927..19152
                     /locus_tag="PLL88_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ00456.1"
                     /translation="MKKFVHVFSIMVILAFVLGSCGPKSADELPTPVVEKTSAPDIEP
                     TALSYLEAWQNRDYATMYGFLSQQSQEVITQEAFTKLLTEFSNNMTLASLVAEVTSTA
                     TSPTSASAKFLVNYATSLFGDFSRQPETNWVLENNEWKLVWDNGAFLPELAGGNTLAL
                     ESRRQERGNIYDRNGEPLVEETTAYALGITPGEIEEGREGQLVYRLSQLTGKTTQSIY
                     ASYEDAGATWYVPVGEVSADEIGNQFNTLLEMGGLVLSEFTSRYYYGGGIAPQAIGYV
                     LGMSEEQQAEYIQKGYLGDESVGQAGLEKYAEEALAGKPSASLYVVNPDGQVVTRISQ
                     ADPTPAQSITTTFDKNLQIETQKAIAGFKGAAIVMEVDSGRILAMASSPNFDPNLFNP
                     SNINSAELLSGVLNDKNQSLLNRATQGLYPLGSVFKIVTMAAALESDLYTADSSYYCS
                     YYFEELPGERFEDWTLDKGYDESGELTLVEGLMRSCNPWFYHLGLDLFRQMGATYLSD
                     MARGFGLGSATGIEQVAEDIGSIPDPKTDGNAVQQGIGQGEMLVTPLQVVRFIAAIAN
                     GGTLYRPQLIESMTSSTGVTTYTFSPEEQGTLPISQTTLETIREGMGLVISSRRGTAH
                     DALSGLNITMYGKTGTATNSTGDPHAWFAGYTDTNREDKPDIAVVVLIENGGEGSEVA
                     APVFRRIIETYYFGEPLKLYPWESEFNVTRTPTLEFTLTPTQTPYREPVKIEPTETPA
                     G"
     gene            19149..20075
                     /gene="rsgA"
                     /locus_tag="PLL88_02465"
     CDS             19149..20075
                     /gene="rsgA"
                     /locus_tag="PLL88_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOJ00457.1"
                     /translation="MSETRAVGMVTRLQAGFYTVRMENSTLTCQLRGRLKQHKKMEDL
                     VALGDRVHVSVIDEEQGVIEEVGERERALVRMAPTTRGIYKQILLANPDQTVFVFACA
                     DPDPSVRMLDRFLVIAEKQKIKALIVANKVDRLDRKRAEEIFSPYAHLGYTVVYTSAT
                     EGSGISELRDHLQGKLSAFAGPSGVGKTSLLNALRPGLGLEVRSVKESSGKGRHTTIA
                     RQLFSLDESSQVADLPGIRQLSVWDIEPEELDGYFVEFPPHVKNCQFSDCSHNREPGC
                     GVRRAVDDGMISTARYDSYLKLRHELAAQRTY"
     gene            20101..22032
                     /locus_tag="PLL88_02470"
     CDS             20101..22032
                     /locus_tag="PLL88_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ00458.1"
                     /translation="MKNKPIRLLIFFATMIAMLSAACSVQGLSKATPTAPEPTAPPLK
                     EITICTAEEPSTLFYYGETNRGSDLIFEAIYDGPFDRVDYLAQEVMMDKVPNIEDGSA
                     SYSPVEAKSGDLIVDVHGNIVNLVEGVQIFPSGCQSMDCAATWDGISQVLLDQLIVHY
                     SVKDGVRWSDGEPVKASDSLFSYQLVGELQNSKYEDVLGKIVDYQVVDAQTVKMSTLP
                     GVVTSDTTAFFFSPLPQHNLESYAAGDLATTDAAARYPMGWGPFKVTDWQPGVSITLE
                     KNEFYFRSAEGYPALDKIIVRFLERGESMQEMISSRACDVVDDSLITKDGSTDLSVLQ
                     DDATVRVSILPGENWKVLLFGIEPASYDDGYYPYGSDRPDLLSDAAFRNAIRQCIDQS
                     AIVAQQSVVGNDQPFVYFPYDTQLTADASTSAAYDPLSAGAVLDGIGWRDYDANPATP
                     RVAYGVANVPDGTVLSLNLYTTTSTDNMQIAGDIASSLTACGIQTTVYPTPATELYAE
                     GPDGILFGRKFDLALITWNTGDELPCALFESDEIPAEDNYWIGANDGGGNIGGYQNAE
                     FDSLCAAARFGSTDPQAALRAQVEAATILDEETPLISLFFDAKVYLMSPQLCLPEKVN
                     NQKYPYSSLEYWDFRDTCN"
     gene            22210..23196
                     /locus_tag="PLL88_02475"
     CDS             22210..23196
                     /locus_tag="PLL88_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00459.1"
                     /translation="MAELFSVKDLETQFHTREGTVHAVNGVSLSLQEGETLGIVGESG
                     CGKSVSMLSTLRLIPQPPGEIVAGKANFQGRDLLELSREELRHVRGAQIGFVFQDPMT
                     SFNPVQTIGKQLMEPLEIHLGMNKKQAWDRAIEMLNMVRIPNPEDRLKDYPHQFSGGM
                     RQRAMIAMALACSPQVLIADEPTTALDVTIQAQIMDLVLSLRKELGMSIIWITHDLGV
                     IAGLADRVIVMYAGYIVEEAKVKDLYVRPEHPYTLGLLRSLPRLDMDEKKRLVSIEGV
                     PPVLFKKPKFCPFASRCPYRVEKCLDENPPLMLVGENHKTACWVDTYTGKER"
     gene            23196..24200
                     /locus_tag="PLL88_02480"
     CDS             23196..24200
                     /locus_tag="PLL88_02480"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOJ00460.1"
                     /translation="MPEREVILHVEDLKMHFPIKRGVIQRTVGYVHAVDGITFDIYQG
                     ETLGLVGESGCGKTTAGRTILQLYKATSGSVIFQGKDLVQLKSEEMRQTRRKIQMIFQ
                     DPYASLNPRMTVEEIIGEPLLVHNIENGKEIREHVKDLLELVGLNPAYVDRYPHEFSG
                     GQRQRIGVARSLALNPDLIVCDEPISALDVSIQAQVVNLLEDLQRELGLTYLFIAHDL
                     SMVRHISSRVIVMYLGILVEMADRNTLYNNPMHPYSIALLSAVPIPDPKVEEKRKRII
                     LEGDVPSPINPPSGCRFRTRCPLAQAVCAEERPEWKEVEAGHYVACHFWYNTKSLQRR
                     "
     gene            24275..26287
                     /locus_tag="PLL88_02485"
     CDS             24275..26287
                     /locus_tag="PLL88_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ00461.1"
                     /translation="MRSKWYVLFSALVILATLLVACQPAAEEAAPGAVEEAADAASFW
                     RTSKDPTTWVETTFGDPETLDTSRDYETGGGEILANVYDTLVFMEKDQASTFVPMLAT
                     EVPSLENGGISEDGLTVTFKIREGVKFHDGSDLTVDDVAFSFVRRILAGGTSSPQWML
                     VEPLLGAGLVDIAEIVDAENPPYDDQATLAAYDAETLASVCELVETKVVADPAAGTVT
                     FNLAQPWAPFIATLANGGWATIESKAWVGANGGWDGDCATWQNYYSVPTEELNQLGIG
                     NSAMGTGPYKFDHWTPGEEIVLTANEDYWMTEPMWEGAPTGAPAIKTVVIKQVAEFST
                     RLAMAQAGDADNILVGSTEDWPILDEMIGGEITYEEYLAGEPVVITDPTKPFYKVSEI
                     LFANNRTDIGFNFNVNVEGGNSFIGSGVLDGDGIPADFFSDVHVRRAFSYCFDYETLA
                     AEVMQGEANRAPVLMLPGMVGYDEDAEHYTYDVEKCVEELQASTWVSEDGVQLYDLGF
                     RMSAVYNVGNTLRQTIAELLQAGLQEAGEQFVVEVVGLPWPTFLANINAKKIPIFIIG
                     WLSDYFDTHNWTSTFTSAYYSFRQNFPAELRAQFSEINSRAVQENDPAERERIYKEEF
                     NTLYFETAPAMLLYTLKGRHYAQPWEKGWYSNPIYSNKWYYALSKD"
     gene            26361..27425
                     /locus_tag="PLL88_02490"
     CDS             26361..27425
                     /locus_tag="PLL88_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00462.1"
                     /translation="MTQYIVRRLLLMPLTLLGVTLLIFGMLSFLSPAERVSLYVRDIP
                     KNDIVMNSLIERYGLDDPFLVQYYHWLVGQKNEVTGEWAGGILRGNFGYSRSANQNIV
                     DLIKRRFPATVELALYSIVPLIGVGTWLGIVAAKNHDKLIDQIARLFGILGYSFPSFV
                     FGLLMLMFFYAKHQIFPPDRLSNWAMLIVSGEGFTNYTSLVTIDALLNGRFDIFLDAL
                     RHMVLPILTLSYINWALYLRIMRSSMLESMNQDYVVTARAKGVKEKDVINKHARPNAM
                     IPIVTMSGMTIAGLLSGVTITETIFIYPGIGSAAAAASTQLDVVTILSLVLLTGFILL
                     IANLIVDITYAFLDPRVRLS"
     gene            27448..28488
                     /locus_tag="PLL88_02495"
     CDS             27448..28488
                     /locus_tag="PLL88_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00463.1"
                     /translation="MAEAQVLDKKLEGLIGEYTERKISRAERILGPEFYRILKGIFTT
                     PSSIVGFTLIAIFVLVAIFAPQIIPPKENTLDPYKIMRDGFGSIPKAPGAVWVKNPPT
                     VPWWYRTFVGDEWVHLFGTASGGWDIFYGVIWGTRTAIKVGIIIEGITLILGIIIGCL
                     AGFYGGFLDTIIMRVVEVFQTFPFIMAAMTLSAILTPIIGKGIWPATIALVVFGWMSY
                     ARLIRGDILSVRERDYILAARVVGAKDSRLMFKHIIPNAIYPTLVVASLDIGTIVVSF
                     AALSFLGIGTEIGYADWGQLVSFARNWIPQLANYWWILIYPGMALVLFVLGWNLVGDA
                     LRDILDPRMRNR"
     gene            28591..28661
                     /locus_tag="PLL88_02500"
     tRNA            28591..28661
                     /locus_tag="PLL88_02500"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28623..28625,aa:Gln,seq:ttg)
     gene            28747..30117
                     /gene="glmU"
                     /locus_tag="PLL88_02505"
     CDS             28747..30117
                     /gene="glmU"
                     /locus_tag="PLL88_02505"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOJ00464.1"
                     /translation="MRIGSVILAAGVSKRMNSAIPKVIHPLMGKPLVWYAIEAAKAVA
                     SVPPVVVIGFAGDKVKETIKETVSYAEQAEQLGTAHAVRMAEDQLKGKVDLVVITSAD
                     MPLVAAETLQRLVDIQSRSGGAMTLVSIQGDESRGFGRIVRDASGNAVAIIEEKVASE
                     EQLAIKEYNAGIYCVQSDWLWDALRRVKKSTVGEFFLTDLLEIAVRDELPVTVLQLDN
                     VEEAIGINNRVHFAEVEKLMRRRINEQHMLSGVTMIDPERVYIEPSVQIGRDTLIFPE
                     TYLRGETHIGENCEIGPGTIIDNSLIGNDCKILKSVLEGAELEDHVEMGPFCRLRTGA
                     HLASHVHMGNFGEVKKSYLGKGTKMGHFSYIGDAQIGENVNIGAGTITCNYDGKNKNK
                     TVIGDNAFIGSDTMLVAPITIGKNAITGAGAVVTRDVNDGEKVVGVPARVIKNRKDEE
                     TNHDSI"
     gene            30104..30514
                     /locus_tag="PLL88_02510"
     CDS             30104..30514
                     /locus_tag="PLL88_02510"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOJ00465.1"
                     /translation="MIQSDIDQKTNLIEAAVEARKKAYVPYSRYPVGAALLTKAGKIY
                     TGANIENAAFPVTICAERTAIFKAVSEGERELQAIAVVTKNGGTPCGSCRQVMAEFNP
                     DLIIYIANEKGEIVQETTLKDILPGYFGPKSLVK"
     gene            30527..31306
                     /gene="recO"
                     /locus_tag="PLL88_02515"
     CDS             30527..31306
                     /gene="recO"
                     /locus_tag="PLL88_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOJ00466.1"
                     /translation="MSDTERLVNSEAYVITQTDYGEADRFYTIFSRKSGKIKALAHGA
                     RKAKSRKAGHLQPLTLIHLMLAKGKTFWVITQADTLQTYSSIKNDLGKTASALYIFEL
                     INRFAPESEPVHPLFQLAKDTMERIEKEQDRFFAIKFFEFRLLKHTGYMPNLTTCVQC
                     GEAILPQDQYFSAEQGGILCPRCGLRGYTVRKVSLPALKYLRFIQRSSYSELGKAKLS
                     TEAKEEMENLMLYYLTYITEKALNTPTFIHQVERAEKEMEQ"
     gene            31303..31557
                     /locus_tag="PLL88_02520"
     CDS             31303..31557
                     /locus_tag="PLL88_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOJ00467.1"
                     /translation="MMNTEKKNFSKEEQIRGLIEQIDAYIDHYHGGSVEFLSLKGNVL
                     TVRLGGACADCSLQPATLKGWVEGTVKQFFPEVTEVLSEK"
     gene            32174..32737
                     /locus_tag="PLL88_02525"
     CDS             32174..32737
                     /locus_tag="PLL88_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOJ00468.1"
                     /translation="MADWVLTLIIAVSLAMDCFAVSLGVGCSLPSCDKRAIFRMSFHF
                     GLFQAGMTLFGWLIGNTILPYIAQFDHWIAFLLLAWIGIRMMREGLSNESEKMTGDPT
                     RGSTLVLLSVATSIDALGVGLSLSILQTNIAFSSAMIGIISLLFTIIGLKFGNGLNHK
                     FGKRIEILGGLVLLFIGSRLLLSHLSG"
     gene            32852..33541
                     /locus_tag="PLL88_02530"
     CDS             32852..33541
                     /locus_tag="PLL88_02530"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOJ00469.1"
                     /translation="MKRMKVMVSFLIMAVVILSACTQAAASEATPDLGAVKTQAVQTA
                     VSQMTADAESGKEAPASTSPTITPLTTISAVVLPTATTRVSYSSGSGGSSGGSSSGTS
                     SGTPIPTATPDVYICEVIGQTPLDGNQYVGADIDVIWTLKNVGVATWTAGTYYYHWTG
                     YSDLSPVHSFTLTQDVPRYSTVQVMIDVRVPIIPGQYRTQWYMVNDNGENFCGFYYYV
                     NAIPLPTATPD"
     gene            complement(33593..34372)
                     /locus_tag="PLL88_02535"
     CDS             complement(33593..34372)
                     /locus_tag="PLL88_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00470.1"
                     /translation="MKSVEREQPAALLPPLPDENPPTQRKSPPWLQIFVIIGLVYGLG
                     VSIFGAAINPTAENLTMVMAIVFSGIYTLLLYLSRKIWAPAVSSEKAVSNAKIFAIAN
                     ALFISFMIKFCAQLIQSSINTESAVGSPNLLLSIPWLVGIILIFIPIQQKYNFSWAVL
                     ILLSGLYELIMEIWLNGFLVPLLSGQSMEIFYIVSDLVIFGYWQFAILYSSVFLVIRW
                     IFEQNPVVEQGKKKPLRHALKPLIWIFPYSVYCALIYFVFA"
     gene            complement(34359..34886)
                     /locus_tag="PLL88_02540"
     CDS             complement(34359..34886)
                     /locus_tag="PLL88_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00471.1"
                     /translation="MNQILKLLSGGDRRSIGRSDEVVELILQQPDKFDDLFRGFYETD
                     PVIRMRTADAVEKVTAQDPALLIPYKEPMLELLESAEQIEVQWHLAQIIPRLPLTNDE
                     ISRAYQTMEKYLSSSSAIVQTCGLQCLFDLSLRDARLLASTRSYLESGMENQSKAVRS
                     RSRNLMKELEKHEKR"
     gene            34940..35437
                     /locus_tag="PLL88_02545"
     CDS             34940..35437
                     /locus_tag="PLL88_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00472.1"
                     /translation="MALRKLSVLFLAVCIALFAGCSQVPKSKPDDFTLYYYWNTGSLP
                     PEYYYQYEIEIDPGGNGIFTLQRGYEEVEENRKAFSFVVPAQDWENFFVWLTENKILR
                     NNWRETADILVGASTTEARIQVNGEKYTIPSVSVLSQRDKGTFYKLEGQIRRLVPNEI
                     WNQVQ"
     gene            35560..36030
                     /locus_tag="PLL88_02550"
     CDS             35560..36030
                     /locus_tag="PLL88_02550"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ00473.1"
                     /translation="MQKNISLVRIDDRLIHGQVTTAWLRVYPADEIIISNEKIANDPI
                     YKAIFSVAQIPGKEILLLSPDDTVERINAMKPGCKYLIITPTPVDIVTLIDKGLQIDK
                     INIGGLQNRPGTTRLAKVVYAKDDEIAAFKALQTRGVEMEVQMVPTEKITKLNL"
     gene            36058..36888
                     /locus_tag="PLL88_02555"
     CDS             36058..36888
                     /locus_tag="PLL88_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOJ00474.1"
                     /translation="MWKEATLVAIVAGGWALVLYYLPQLITVLSKKESKAMTWWLLLM
                     RGVNDAFSYAVVIPAALTGWVLGDLRTGLIVGGTVQAMFIGVFIVGASIPPNPYLASI
                     LSTALVILTGASTGEALALVVPIAVVSQLLTLAFMSLNVVLCHWGDKMAADGNAKGID
                     ILNTLDGTGWTLVNVIPAFLGVGLGIDAAAKLLDIIPGWLADGLSYAGGILPALGIAM
                     LLLIIASKEVWPFFFLGFIAAVYLNLNAVAGAILGVVIAIIFVQLKSAKEEGTKEKGI
                     "
     gene            36902..37714
                     /locus_tag="PLL88_02560"
     CDS             36902..37714
                     /locus_tag="PLL88_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOJ00475.1"
                     /translation="MKKLDEKSLYGALWRVNNFAGNYNFERLMALGMTQVMVPVFRGL
                     GITGDKLKEGLKRHMVFYNTHPYFGALIMGVMVAMEEAKANDDAGVTEEAINGFKVAL
                     MGPFAGIGDSFFWGTVHPIILAVSANLALQGNFLGFLFAFLIAAEATLGTFYPFIWGY
                     KSGTEIVEKIRTSKLIQKFTEGAKIVAMFVAGVMVTTLISISTPVVLFASEAEGSGVN
                     LQGIFDSILPKLLPLGLLFFVYWLLKKKINPLWVMLILMVLTVGLKALGWVG"
     gene            37789..38805
                     /locus_tag="PLL88_02565"
     CDS             37789..38805
                     /locus_tag="PLL88_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ00476.1"
                     /translation="MRAAVYTEPRKIVLDANYPDPEMGDGVMIRVRASGVCATDIKAF
                     KGLRFGMEPPMILGHEFAGEVLVSSMDDYKIGERVSAAPYAGCGFCNYCLDDHEELCQ
                     DKDFTTNGCFAEKLFIPRDLMYKTGWKIKSDVHYQAAALAEPLACVILSLESCHWQPG
                     WTIMVVGGGFMGILHVILAKAWGATKILLSEPNAKRRAFAEKLGAIVCDPSEEESLGD
                     FARKNVDGLGPHVVIASIANEQVVESAFDCPRAGGYYHMFGGLPKQSTITIPSFLTHY
                     KEVALVGTSGFRTKDYRLASRLINEKRIDLEPVLSSLFRLDQVGEAIEQVEQQTILKA
                     MIVE"
     gene            38811..39215
                     /locus_tag="PLL88_02570"
     CDS             38811..39215
                     /locus_tag="PLL88_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ00477.1"
                     /translation="MTDRIPTPIMLISHDGLAAAMVRASAMIMGEQENIGVVGLEEGD
                     GLSNFIGKVQDNIADLKDAGQGIIFLVDIPFGTPWNASVAVKREIDHIVAGVSLPFFL
                     EVLNYRNQYNEIEKILTDSLSCKDQCSVIWVH"
     gene            39239..>39913
                     /locus_tag="PLL88_02575"
     CDS             39239..>39913
                     /locus_tag="PLL88_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00478.1"
                     /translation="MEFGKQRRMQRIFNTDGRTLVVAMDHAAYMPDVVLGLQDPGRYI
                     QEIHAAGADAVMTTLGTIRRIQSKIIDFPVILSLESGYTNIGDFVEQGLRMGVDMVKC
                     MVYPFSAEDPSSLNNFARLANAADAWGMPVMAEVFPGGMNGGKEWNTVEKLSSCIRVT
                     AEAGADVIKTFYLDNTGDFHNVIENSLVPLVVLGGVKSDDPLPLLERIALCLEKGASG
                     VAIGRNI"
BASE COUNT        10248 a         9299 c        10609 g         9747 t
ORIGIN      
        1 taataaaact gcaccttttc tgttggttgg tgtccaactt ttggggtgca gttcatcgca
       61 cggctctttt gactttaaag catggaacgg aaggggatgt tctgaggggc ttcgaagcaa
      121 tcttcgaaac cgcggcgatg gtggtaggcg cgtttgtctt tatcggtggg atatatatat
      181 ttgccaccaa cttcccagaa gaaaggatgg aactgatatc ccaggtggtc tttcttcatt
      241 tcgtacagga ccttgatctc attgacgtca gccatgaaat ttgtccagac gtcgtaatgg
      301 atggggatga ccactttaca gcgcagggct tctgccatac gcagaatatc aatgctggtc
      361 atcttatcag ccatgccaac cggattttcg ccataagagc cgaacgcgac atcgatcttg
      421 tggtctttgc cgtgtttggc ataataaata gaataatggg agtcgccgga atggtaaatg
      481 tttccgccgg gggtcttgag caggtaattg acggctttct cgtccatatc ggtggggcaa
      541 acgttggtga gttcttcgcg atcggcgtcg gtggagtcgg tggtgacgag gcaggtgcgg
      601 tcgaaagaat ccagcacgac gatctcgatg tctttgacct tgatgaaatc gccgggtttg
      661 acggtgatgc aacgatcttc agggacgccc catttcaccc aggtttcgac gctctttttc
      721 ggaccgataa aagggacggg aatttgttta ccagccgcat cgacggtggt gaggttgctc
      781 tgcagcacat gggcggcata ttcaacgctc atatggtcct ggtgataatg ggtggctaaa
      841 actgcgtcaa gctgctttac ggcgaagggg tcaatgacga aaggaattgc ccgcaggttt
      901 ggctgcatca tgcgcgcgcc gcacatattc gccatctgat gaccgacttt cattttgcca
      961 tcgccatggg tgcgcttgcc attgccacac cacaggtcaa tgctgatatt ggcgccagcg
     1021 ggtgtcttca tccaaatgcc ggtacagccc agccaccaca tggctacggt attcggagcg
     1081 accacttcat tttcaatttc ttcgttcagc catgttcccc attcgggaaa tgtgctcatg
     1141 atccatttct cacgggtcat ttcagatact ttgctcattt tttctcctta tgattctaaa
     1201 agtttcttgc ttctgttgtt aaccattaat ttcgtcaaga ctctcctgtt gtgccaggcg
     1261 gtgccactac atccatccag gaacggcatg atggctggat gctccgatgg tgagatcatg
     1321 accttgtttc tcgaccagcg cgtcaaatga ttgtggtcaa attgtatatt aaaagtagta
     1381 tttatggaaa gaggaaagtc atctggtaga agaaatctgt gcattttttg aaaaaatctt
     1441 caatggaagg ctgtttgaac agacagtggg ttttcttaaa aaaaccacct ctatggaggc
     1501 ggtttttgca tacagggatc agcgaggggt gcaccggaag attatttttc tccccggctg
     1561 tcaacagagc ccagcccgct gatgttttgg gtgacggatg gagagccata atagcccaca
     1621 gagcccacgc ctgaaacctc gatatcgaga acatcggtga cccacacttc agcgctgccc
     1681 gcgccgctca tcaccacgct ggcattgctg ctttgcaggt ttggagcatg atagctgccg
     1741 acgccactga tgatcacatt ttgattgagc gcagtaccgg atatatcaat ggctcccgca
     1801 gagttcagca gaacggtcag ttgccgggta ttcaaagcag tgacatcaat actgccggca
     1861 ccggtcaggg taatttccag gtggtcggta tcgagggaag cgatggaaat gctgccggct
     1921 ccagataacg aaagttcatt tagatccttc accgtcaaag tgtagacggt gcgaccaagc
     1981 gaatctccga gtacgccgcg cgttgaattg atatacagcg tgtcgccgac cacctcggta
     2041 gaaatattgc ggaggatgtt gttaggaccc tggatggtca gtgactcttc gtccccttgc
     2101 cgaatgatga atttgccgac cgtgttgatt gagatggcgc tgacgccatt gatctcgcgc
     2161 gtttccgtgc gctcaactcc gaccgagcac gcgctcaaaa acgcgaggaa caaagcgccg
     2221 accagcgtag acaataggat ttttcttttc atatctgcac tcctatatac tgattgatct
     2281 gtcaattcca ttatttaata cggatttgga ggaaagtgga tgcgcccaac gcggggatat
     2341 tatagaggtt tgccgcagga gccacaaaaa ttgttctccg gggggttatc ggcgccacac
     2401 gctttgcaaa ccacctggga gcgcttgttc tttttccatt tctttttcca tgggttggaa
     2461 gtatccatag tgatggcttt ggtctttttt gcgatccaga tcaaaaagat ctccgaaaag
     2521 atgatcaatc cgatagcgac gatcatggaa attatcacag gtcttgataa catggtggtg
     2581 atattgtatt ccaaattttg gttgggcggg ggcacggaaa ccccgtgcgc ggcgttacag
     2641 gaaaaattga cagcgaggac agaggtgtta taatgtctct aatcgtgcat caataaacat
     2701 tttcttgtcg aagaatgtac tattaattct gaaaaggtcg ttgccatgtt atcggatgaa
     2761 cggcgtgccg ttttgatcaa cagattgcgg gaagacggat atgttcaagc cgcggagatc
     2821 gctgaagagc tgagcgtttc cacggctacc atccggagag atctggtgtt cttgcagcaa
     2881 gaagggtttt gcatgcgcac gcgtggggga gccgtgcgct cttcgcaggg caccaccctg
     2941 gagttgccct acgctttaaa aaaacaaaag ttcgtggcag aaaaagaagc gatcgcgcgg
     3001 gaagccgtca ggctgatcga aaatggcgac accttgatcc tcgattcagg ttccacgacc
     3061 tatgcgctcg ccagcctgct gactgagaag caacgcctga cggtggtgac gaacgacttg
     3121 cagattgcta ttctgctggc ttccaatccc agcgtccatc tggtgtgcac cggcggtatc
     3181 gcgcgtccgc atgtgttcac cctgcaagga agtgaagtgg tgaatttcat caaaacgtta
     3241 cgggtcgata aaacatttct gggcactgac gccattcatg aagatggaac gatcggcaat
     3301 gtgaacatgg aagaagtggc gatcaagcag gcgatgatga aggcggcatc aacggtgatc
     3361 ttgctggctg attcttccaa attcaccgtg aagggatttg cgcaggttgg gacgctggct
     3421 gaggtggata ttctgatcac ggacgcaaag tatccgaaag aaatggcgac cgtcattcag
     3481 gaaaatgcga tcaaactggt tgtggcaaag tgaagaacac gatgataaaa ctctccccat
     3541 ctctggcttc cgcttctttt ttggatctgc aaaacacggt caggcagctc gaaaaagccg
     3601 gagcggatta tctccatttt gacgtggaag atggatgctt cgtaaaagaa atgcgattgg
     3661 ggatcaaggt cattcaagac ctgcgggcta tcaccgatct gccttttgat gtacacctga
     3721 tgatggtcga ccccgaatgg atcgtgcccg tactggcgaa gatgggggtc gaactgcttt
     3781 cggtgcattt cgaagccacc gagtatccga ggagaacact tggcatcatc caggatgccg
     3841 ggatgaaagc cggtttggcg ttcaatccca aaaccgcgat ccctgatctg tccttttatt
     3901 tcccttacct gcacttcgtg aacgtgttga ccaccgaacc agaagtgaag aactgcgctt
     3961 atctgccgca ggttttaaca aaggtgcgcc aggttaaaag cgcgtatgcc gcacagggtg
     4021 tgctttgcga agtagatggc gggttctctc ctgaaaatgt gctcgatgct atccatgcgg
     4081 gcgcagatat catcgtggca gggcgaggca tctttgggga cagcgagatc gaaaacaatc
     4141 tacgcgccat gaagtcggct ggttgaaaac ggcttgggaa acaaaagcac aaccatcctt
     4201 ccatggttgt gctttttcaa gggggtaaaa agacctgatg tttaaaaatt cctgcggaaa
     4261 tcttttacct tgtccataaa agttttcacg cgctgcacat ccacggcgtt ttgaaaaatt
     4321 ccatcgacct tgaaggtggt ggcaacgact gcgccatccg caatgctgag ctgctcttcc
     4381 acgttctcca gtcgcatgcc ggtgttggca aacaccaccg tgttggggac agccgatttg
     4441 accaatttca acgtggcggc atcggtctgc actcctgccg tcaaaccgga cacgcatagc
     4501 gcatcgggca gatggttgaa taccgttgag cgggcgatcg aaccgatatc gcgcggggtg
     4561 aggtattggg cggcttcggg aacgatgttg aacagcagct tgacattctc cgctcccagc
     4621 gcgtgttggt gcctgaccac ggttccgcag ttggtgttcc ataatccgaa atcgctggcg
     4681 tagactcctg aaaagatttc tcgcacgaat ttcgcatcca cagccgcggc gaggtcaagc
     4741 gaagcgtagg gatcccaaag cacgttcacg ccgtgaggaa ccgtaatctc atgcatcaat
     4801 tctccgatga cgcgccccat ggcggcgatg gtttcgggat tgaccttgag cagatagggc
     4861 agactgaatt cgttggaaaa catgacccca tccacgccgc cttcctggag agccagcagg
     4921 tcggctctgg cgagggcgat gacttttttc attccagcaa ccctgtcata tgcgggatct
     4981 ccgggcagcg cttgcagatg gcacatggca atgatgggtt tgtttgtgcg aaaaacttct
     5041 tcggtccaat atggtttcat tttttctcca ttcattaaaa tctttggcaa taccttgtaa
     5101 tacctggcac agaaacaaac aaataaatgt ataataatgt gcaataaacg acattataac
     5161 acaagacgaa aattaacaga aattatggag aaacgacatg cttgaagcgt taaaaaaaga
     5221 aatggtcgaa ttgcttaaag aactgcccca gaacgggctg gtttgtctga ccagcggaaa
     5281 tgtcagcgcc cgggatgcaa aaacagggct ggtgatgatc aagcccagcg gggttcccta
     5341 tgaacaatta gacgagtcca agctggttgt gctgaacctg gacggaaaag ttgttgaggg
     5401 tagttataaa ccgtcttccg acaccgccag ccatttgtac atctatcaaa atcgccaaga
     5461 cgtgaacgga atcgtgcata cccattctgc gtatgccacg gtgtttgccg cccttggaaa
     5521 gaacataccg gtgttgacca ctgaaactgc agaagagttc gggggggaga tcccctgcgc
     5581 cgaattcgtt ttgatcgggg atgacgaaat cggcaagcag gttgtgctgt atggcgagcg
     5641 gtgcaacgcg gtgctgctga agaagcatgg ggtcttcact ttcgccgcca ccccgcgcag
     5701 ggcggtggac ctggcgatcc ttaccgagaa ttctgcccgc atcgcctggc tgatcctgca
     5761 aatgggcgag ccggatggca ttggggcgga ggaaatcaaa gcgctgtatt accgccaaca
     5821 gaacatatac ggtcaatagc tagatccatt ttccgatcat gctttccatc ccgccaacgg
     5881 tgaagaattt gctttcctat tatcggaaat atgttctata atagaaacca caggtgatgg
     5941 ttatggatct ttttgattat caactgtcgg tgaacctgag caaccaggcg cccctggcgg
     6001 cacgtatgcg cccgcgcacc tttgccgagt tcttcgggca ggaacacatc gtaggggaag
     6061 gaaaaatgct gcagcgggcg gtactggctg accggctttt ttcttcgatc attttatggg
     6121 gtccgcccgg caccggcaag accactctgg cacagatcat tgccaattac agcaaatcat
     6181 atttcgtgtc catttcggcg gtgatggcgg gcaaggcaga gttaaaatcc gtcatcgatg
     6241 aggcgctcga acgccgccgc ctgtataaca aacgcaccat cctgttcgta gacgaagtgc
     6301 atcgctggaa ccgcgcccag caggatgcgc tgctgcccaa cattgaaaac ggattgatca
     6361 tcctgatcgg cgcgaccacg caaaacccgt attttgatgt gatcccagcg ctggtttctc
     6421 gctcgcgggt cttttcctta aaaccgctgg atgaaaaggc actggcggct attttcgaaa
     6481 acaccctgaa ggataaagaa cgggggtacg gtaacattcc cattgatatc gatgacgacg
     6541 cgcgcgagca tctcattcag atctgcggcg gggatgcgcg caacctgctc aacgccttgg
     6601 aactggcggt ggagagcacc ccccggggag aagacgggcg cctgcacatt tcgctgcagg
     6661 atgcgcaaga atccattcaa aagcgggctg tgcgctatga tcgtgccgcc gacgaacatt
     6721 acgataccat ttccgcgttc ataaaatcgg tgcgcggctc tgacccggac gcggctttgt
     6781 actggctggc aaaaatgttg tacgccggcg aagatccgcg tttcatcttg cggcgactgc
     6841 tgatcctggc tggggaggat attgggttgg ctgacccgca ggcgctacag gtggcggcat
     6901 cggcgacgta tgcctttgaa tatgtgggct tgcccgaggg cgtttttccg atcgtggaag
     6961 ccacgctcta cctggcaacc gctccgaagt cgaactctgc gctgggctac ttcactgcgc
     7021 taaaggagat cgaagaaaag ggagcgcagg cggtgccaat ccatctgatg gatggcaatc
     7081 gcgacagcaa ggctctggga catgggaaag gctaccgcta tccgcatgaa tttcccgacc
     7141 attttgtggc gcagcaatat ttgcccacag atttgatcgg aaagacattt tatgaaccat
     7201 cggatcaggg aagggaaaaa gcgatagcag aagcccttgc caggtggcgc gagatgcaag
     7261 acagggtctt gaaagaccaa ttttcgaacc aagcccccta acgacttatt cctgaagaaa
     7321 aggaatgagc gcttgcaccg cttttgcccg atgactgatg gaattctttt ccggcagggt
     7381 cagttcagcc atggttttcc gggttccctg caccagaaag agcgggtcgt acccgaaccc
     7441 gtattcccct cgttcttccg ggatgatctc gcccacgcac tcgccttcca tagaaatggg
     7501 ggaaccgccc gggatgacca gagtggcaac acagatgaaa cgagctttcc agggacgtgg
     7561 ataagcgctt aatttctcta acaagagttt gcgccggtcg ccatcggagg cgtgagggga
     7621 attgacaaag cgcgctgaat ggatgccggg caaccctgag agggctgcca cctccaaacc
     7681 ggtatcatca gaaagcacgg gcagttggct ggcgcggctg aaagccagcg ctttgatttc
     7741 tgcattttca cgataactgg aaccggtttc atcgacatcc aggtttaacc cgatttcagc
     7801 gggggtgacc agatgcgcgg gaatattgga gaggatgctc ttgatttctt tgatttttcc
     7861 gagattggtg gttgcaatta gaagttgcat agtatttttg ccttaatatt tatgaaaaat
     7921 atcataaaaa cttgaccgac tagaactggc gtgctataat cgagccagca tcaatatttt
     7981 aacggattta ttgtgaaaca accccgcaga ccttttcgaa tcaatgtcgg atttctcatt
     8041 aaccaaccca ttggtttcag ccgggaaata cccttttcct ttgagagcta ccaatttcca
     8101 aatgatgaga agatggaaga cttgtgcggg acgatcgtgt tgatcagaac ccaaacaggc
     8161 atccaggcgt tgatggattt ttcagcagta gtggacgcgc agtgtgtgcg ctgcctggaa
     8221 gacttcaagc aggcgctgca taccagtttt caagaattgt atacgtattc tctcgaacca
     8281 ctttctgaag atgaaaaatc gatccccgaa gatggctact tagatttcga agaatccatc
     8341 agcgattata ttctgcttga atttccaata aaacccctgt gcaaagctga ttgccgtggg
     8401 ttgtgcatgg tttgtggtca aaacttgaat ctttcatcct gtgagcatgt tgcgcaagcg
     8461 tgacatccga cagtcaatac cggcaagacc ggaatcccat ccttatggga ggtaatcgtt
     8521 gtcgtacgat gaacaaaaaa taaaagacga catccttgaa atccttctgg aacaatcgga
     8581 aacccacgat gaattgtcca tggaggatgt ttataacgcg cttccggata acgaagaatt
     8641 cgatcaggaa ttcatctatg gtctggtggg tcaactgcgc aagcaagggg tggaagtcgc
     8701 ggaagaagag ggggaaccgc tggcggacga agctgagata cccctcaccg acatggaccc
     8761 ctacgttggt ttaagccata ttgccagtga tgatacgatc tctctctacc tgcgggaaat
     8821 gtcgcgcgtg cctctgctgg atagcaacga agaacgcaat ctggctattc gcattgaaaa
     8881 tggcgaagca gctcagatcg agttgagtca attaaatgga aatgcgaccc cccagcaaaa
     8941 agaagaactg gagaggatca ttgcgagcgg aaaagaagcc cgcgcccatc tgatcaaagc
     9001 caacacccgt ctggtggtca gcattgccaa gcgctacacc aaccgaggtg tgccatttct
     9061 ggacttgatc caagagggca atttgggatt gatgaaggcg gtggaaaagt tcgactacac
     9121 caagggtttc cggttttcaa cgtacgccac ctggtggatt cgccaaacca tcacccgcgc
     9181 catcgcagac caaggaagaa ccatacgagt tccagtgcac atggtggacc gcatcaggca
     9241 gatgtacaag cagatccacg aattggaaca gaccctgggc agaacgccca ccatcgacga
     9301 gctgtccaaa gccttgggga ccgacaagca gaaagtgtcg tggatgctgc gcgtttcctg
     9361 gctgccactt tcgctggaaa gccccatcaa tgaggatgag gatgaatctg aactgggtca
     9421 tttcgtggaa gataaagaaa cccccacccc catccaaacc gtttactcca agttgctcaa
     9481 agaaaaggta aacgatgtac ttgatacgtt gccgtgccgc gaagcacgca ttctgaaact
     9541 gcgttttggc ttggaagatg ggaagtatta cacactcgaa gaggtcggca aaaaattcgg
     9601 gttaacccgc gagcgcattc gccaattgga aaccaaagcg ctgcgccgat tgcgccaacc
     9661 acaaaaatca caacagttgc gcgactaccg ccaataacag ctagttttga ctgaaaaagt
     9721 ctgccaggta cagccgcacc agttcaggat attcccaaag gaaaagatga cccgcattgt
     9781 gaatctcttt gatcacgggc tcagcaaagc tggcaagcat gctttgtttt tcttccacat
     9841 tcatgaaggg atccgcagcg ccattcaaga acatgaccgg ggtgggtagt ttctcatcgg
     9901 gaaagcgggg atgataggtg tgtgtgatcc aaagatcatg tttcaatgtc gccgcatctt
     9961 caaccaggaa gggttgaagg agcttttctt tttgcgagtt cgaaaaacca ccagcgagca
    10021 atttatccat gaatacctgc tgccaatcca ccgacagctc attctgggga ccatgcggtg
    10081 cggaaatctc gttgaatgat ggttgcggcg cgtacgccag taatgccgcg cgacaacata
    10141 tagcgggaaa tcgaagaagc aattgcaggg cgatggcaga tcccatggaa taaccgacca
    10201 aattcacgcg cttcatctcc aaacccaaaa ggaaagcgtg caaatctacg gcatattctt
    10261 caatggaatg gagatccttt ttctccgctg ccccatgatt gggaaggtca atggtcaaga
    10321 tgtgcatgcc gggcaggcgt ctgatcgctg ctggccagcc caggtgcgaa ctgccagccc
    10381 catggatcaa gacggtcggc aacagcgcag accgatcagc atgaaattct tgatacgcgt
    10441 atccattgga aaaatgcatg gatatcctgc ttataccctg cgtagcatat acaattttcc
    10501 acccgtgaat cccctgttga aataataatg ccgggtgcca atggctgcga taactgccag
    10561 ttgttggatg ccccgttctc ggcacaattc caccgctttt tcgatcaggc ggctgcccaa
    10621 gccaacgtgc tgggcggcgc cggtgttctc cgtgccgaac cgcaaggatt gaccataaat
    10681 atgaacttcg cgaatgatag cgcaaccgcg caattcgggt atgtgttggt acacctgttg
    10741 gtaaacctgc tgcccctgtt catgggtgga atctggaaaa gacaggcgca gataaccagc
    10801 caggtggtca tcgggagtgc ggtagtgtaa gaaatgctct tcggaacagg cggggtgata
    10861 aacaacctca tccaacatga gcgtttcttc atccacaagt tttccgcgga tctcacggca
    10921 gcggacgcag cggcagcgtt ctccccgtgc tttgagttca ttttgtacat cttgccttaa
    10981 actggagcgt ttgttcccat cgacgatgta ggtggcagga atatcacgaa tgacgcggtt
    11041 cacgcgacag tattccggaa tagacagctt aacatctgcg atgagctgga ccagctcttg
    11101 ggtggtataa ggtttataag cgtcccgctg ccagtactgg taaagctggc tgttctcgac
    11161 caactgagtg ggataaatct tgatctcatc cggacaataa ccgccttccc acattttctt
    11221 aaaatcatcc cggtctgacg caatggtagc ccccatgaga ttgggcatcc agtgcatgac
    11281 gatcttgaaa ccagcggcgc gcaacagagc gatagcctgc aatgtatccg cggcggtatg
    11341 accgcgcctg ttcattgcga gaatgcgatc gtcaaagctt tgtgccccga tttgcacctt
    11401 ggtgacgcca agctggcgca aacgagccag aacagccgca tctatcgcat cggggcgggt
    11461 ctcgatcacc agcccaacat tgcgccgttc ccctgcaacg ttcatggctt gtgcttcttc
    11521 caatgaagtc gaatcgaccc cgttcatcgc gtcgagacag cgctggataa accaggtctg
    11581 gtaatcagca gggtaggcgg tccagcagcc gccaaggatg agcaattcga tcttactggt
    11641 gggatgcccg atggcatcat aggaagccag gcgtgaagat acctgcaaga aaggatcaaa
    11701 ttggttttcg aaggcgcgcg ctgcacctgg ttcttctcgc agataacttt tggggagctc
    11761 ctgatcatca gggcagaaaa tgcaatcgcc cgggcaggga aacggcttgg taagcacggt
    11821 gaccgtggta acacccgata aggaacggat cggcttcatg cggattgcgg agaggaaaac
    11881 aggatcatcc gggtacgtcc cggtttgcac caattgctga tattgtgcga taagggcgtg
    11941 cttggcgata tatccaccag cttttaaggg gtgagcgtgg atagccttca tcagcggcgc
    12001 gcccgctttg acctcatcca aaaccaggcg ggcaatggct ctttcttccg gagtatattg
    12061 tttggctgtt cgccaagcct cttttgtttt tttcatttct attcgggcaa ttacactata
    12121 atgacatggt ggatgaactt acgatccagc gcattcatgc tcttaaccgc gaattctatc
    12181 agactttttc gggttcattt tcgtccagcc gctaccaggt gcaggcgggg gtgcgacgga
    12241 tcgtgcgaac tttccccact tcagggtcaa ttctcgacct gggttgcggg aatggaaaca
    12301 tcttgcgcga attggctgcg gtagggtttc agggcactta tcacggcatt gatttcagcg
    12361 aagccctcgt gaatcatgcg cgaaatttct gtgaggcaat gcagccaggg gcagcttttc
    12421 gagcgcagtt cgagacgcac gacctgatat ctccttcatg gaagaactcc ctcaccgccc
    12481 agcagtatga gcgcatctgt gcctttgcgg tgttgcacca cattgcggga ggtgaaaaac
    12541 gcaaagctgt ttttcaagca atccgctcct tgatgagccc tgtcacggaa ttccatttca
    12601 gcgtctggca gccacagaac agcaagcgct tgttgaagcg gatactaccg tgggatactg
    12661 tggacatcga tgaaaaaaaa ctagaagcgg gggatatttt gttagactgg ctggcggaag
    12721 aggaaagcag gcatagaacc ggttaccgct atgtgcatgt tttttccatc gaagaattgg
    12781 ctgaaattgc gaaaaccctg ggattcaggg ttgttggaag tttcttttcg gatggagaaa
    12841 gcaataatct cggcttgtac cagcactggc gagcgacagc gcttccttga tctcatatat
    12901 tgttttaaga aaaattcgct acaataattc gatacaatag taagaaaagc ataagcataa
    12961 gaaggagcaa gaaaatggct ggacaagaag ttgatttatt aaagattttt aatcaggtct
    13021 caaaaacgtt aaagaccaat caagatgcgc ttaaccaggc agacgaatat aaccatgacc
    13081 atggggatca catggtggaa atattcgaag tgatcacctc tgccatgaaa accaaaaaga
    13141 acgccgaccc ggctgaccaa ctggattatg cagcccaact gctgcgccaa aaatcatcca
    13201 gcggttcggc aacaacatat gccaacggat tgacgcaagc ttccaaacaa atcagcggga
    13261 aatccatcaa tatagacacg gttctccccc tgctgcagac gttgttgagt gggggggaga
    13321 caggggcatc cagcagcggc ggactcctgg gggatctgct gggaacgttt ttgggcggcg
    13381 cttcatcgac gacaaccagc ggcgggagcg gtggaataga cctcagcgat ctgcttggtg
    13441 ccggtttatc ctatatgcaa tccaaacaag ccggcagttc tgacatggaa gcgttgtcaa
    13501 aagtgttatt gggcgggtcg caatacagca gcaatccata tcgaacccaa tcaggagaat
    13561 tgatcgccaa taccgtgctg caggctctca gcggtttgat gagcaagtag cgaaaacgaa
    13621 aagccaattt gaacaagagt tggcttttct gtttaaatca aacgcacgta taatcttttg
    13681 aaactaacaa taaaggatag acaatgccgt accttttcat agccctgggg atcgtattgc
    13741 tcgtttttct tttcatggtc ctgcgcaccc tccagctatc ggggagtccc catcagcctt
    13801 ccagcgctga cctgatgcag gtagaggtgg gaaaacccgc ggagcatctg gctgaagcgc
    13861 tcagattcaa aaccatttca aaattgaaaa tggaagctgc agatagcgaa ccattcaaaa
    13921 aatatcacct gtggcttgaa aaaacctacc ccaaaactca cacaattctg aaaaaagaaa
    13981 tcgtgaacca gttgagcctt ctctactgtt ggaagggaag cgaatcgcgc ttgcaaccag
    14041 ttctttttgc aagccacctg gatgtggttc cggtcgacga agcgaccctt cccgactggc
    14101 atgccgatcc gttcaaaggg gagatcaagc aaggttttgt gtgggggaga ggctccctgg
    14161 acatgaaaaa tcatacaatc gcattattcg aggcggtaga agggttgatc gtagcgggct
    14221 ttcaaccaaa acgcgatatt tactttgcat tcggacatga cgaggagatc tcggggaaag
    14281 atggtgcgaa aagcatggca gcccttctta agaggaaggg gatacgattg gcggcagttt
    14341 tggatgaagg cggcatgatc agccatggtt tggtccctgg ggtggaaacc ccggtggggc
    14401 tgatcggaat tggagaaaaa gaatatgtaa cggtgaatct gatagcaaag ggaacgccgg
    14461 gtcattcctc caatcccccc cgccaaactg ccatcggcgt attggcacgc gcgatcgcct
    14521 tgatggatga tcatcccttt ccggcaagac cagccctggc aattgccacg ttgcgaggga
    14581 tcgcatacct gtttccgttc gcggttcgat tcgcgcttnn nnnnnnnncc tggctgttaa
    14641 agcgcgcatt ggtcaagcag ttggagcaga aagtacaaac aaacgcgtta attcgtacta
    14701 cccatgcggc gactgttttt aatggaggga tcaaggataa cgtactgcca gccgaagcac
    14761 gcgccaaggt gaactttcgc ctgctgccgg gagatactca ggatactgtg ctgcagcata
    14821 tcactaaagt cgtggatgac ccacgggttc agacggaaat cgatctggct gacgcgtggg
    14881 gaccttgcaa ggtttctgcg atcgattccc ctgcctacaa aaccctggaa cttgtcatca
    14941 ggcaggtctt tggcgaaatg ccggtagccc cttacctgct gatgttcgcc accgatgccc
    15001 ggcactacca agatgtcagc gagcagctct accacttctc tccgcttgcc atggaaagcg
    15061 atgatatcgc cagaatgcac gggatcgatg aaaggatcag cgtggaagcg ttgtctggca
    15121 tggtgaaatt ctacgcacgt ctgatgaaag tgtggggcga ggcggagttc taaaaaatca
    15181 ccttttggtg agcgtgatag agtaaaccaa tgcatcaagc gctccgaatt tgtatttgta
    15241 cgcttcgttc ccgcgcatga aatccagcgc tgcgcgagaa ttctcaatcg accagcggat
    15301 gagatggctt aacaagaccc agccgggcga gaaatactgc agacggctgt caaagccgga
    15361 attgtagata tagatggtgt tctgatcatc aaagcaaaag aaaccagatg caaggacgcc
    15421 gttaatctcc aggaaacata acttgaggat cccctcggca taactccagg aaagcaacct
    15481 ttgcatgtga gctgccatac tggttgtgag aaacgcgttc tttgcggggt ctttgcgcat
    15541 gaggtctaga aaggcgagcg ccattttctc gaggtcattc tggtcatcta caaaaaagaa
    15601 acgatgctgc tgcgcttctt gttcgacccg gcgcagcttc ctgcggatct catggcgctg
    15661 ttttttgtcc aagctttcca agtaagcgtc ccaggagacg ggtaaggtca gacagggtgc
    15721 cgggctgtct ttctccacat ggcatgcgat accatccacc atcgcctcat tttggaaaaa
    15781 ttgaagcaac ggtgaagatt ggtgcacgtt ggagaattcg accctgtccc acttttcagg
    15841 aatgtcttga agcaggtggt ggagtacggc gctgataaaa tcatgaaaat aatcgctggg
    15901 aacgatcaag tccaggtagt cggaaatttc tttggcgccc aggaatgtga gaatattctt
    15961 gccatcgcga tgcgataaaa acagaggcgc gatcgccacc aggttctcct gttggtagcc
    16021 aagcatgata taaggttctc caacctgcca ttctcccccg cccaacgttt gccaccacag
    16081 tttcaagaag ccgtatctaa gaaatggcac gttgatggaa cttttctgta ataacgcgtc
    16141 ccattctgac tgttcaatat catcaaaaga attgcatact tttatcttca tggtcgtaaa
    16201 gccgtatcgt tcatcaggta attataccgg gtacatcatg atacaatctg ggcgatatgc
    16261 aacaaccgca aatactactg gcttcctcct ccccccggcg cagagagctg atggcgtgga
    16321 tgaaaatgcc gtttacggcg acttcggcgg atattgatga aagtcgtcga aaggatgaaa
    16381 atgccctcga gcacgtgcgc cggttggcgc gagaaaaagc cgagaaagtc ttcgaggaaa
    16441 aacacaggga ggcagttatc ctggcagccg atacgatcgt gatcaaaaat gaccacgtcc
    16501 ttggaaagcc acagactgaa agacaagctg tggaaatgct ggtcgaactg cgaaagacga
    16561 cccatcaggt tgtcactgcc atttgcatca tccaaccaga cggaatcagg cgagaggatg
    16621 tgtgcatttc tgaggtgctg atgcgcgact attccgatga agaaatacaa cggtatgttg
    16681 caagcggaga tccaatggat aaggcgggtg catacgcgat ccaaaatgga acgttcaatc
    16741 cggcacccga attcaaaggg tgctttgcca gcgtcatggg ctttccattc tgccatattg
    16801 aaaggaattt ccgaaagata gatgggtata attggatacc ggttacagca atatgccaac
    16861 agaatcttcg gtatgtctgc ccgatctttg cgaaagtttt gaatggcgaa aatataggat
    16921 aagagaatga aaaaatttgt tcatgtgttt tccatcatgg tgatcctggc ttttgttctt
    16981 ggaagctgcg gacccaaaag cgcggatgaa cttcctacgc cggtggtcga aaaaaccagc
    17041 gcacccgata ttgagccaac agctttgagc tacctggaag catggcaaaa cagggattat
    17101 gcgaccatgt acggttttct ttcgcagcaa agtcaggaag tgataaccca ggaagcgttc
    17161 acgaaattat taacagagtt ttcaaacaac atgaccctgg cttctctggt tgctgaagta
    17221 acctcaacgg ctaccagccc aaccagcgcc agcgcaaaat tccttgtcaa ttatgcaaca
    17281 tctttgttcg gtgatttcag caggcagccc gaaacgaact gggtactgga aaacaacgaa
    17341 tggaaactgg tgtgggataa cggcgctttt ttaccggaat tggcgggtgg aaatacgctc
    17401 gcgctcgaat ccagaaggca ggagcgtggc aatatctatg atcgcaacgg tgaaccgctg
    17461 gtagaagaga cgaccgcgta tgccttaggg atcacccccg gtgagatcga ggaaggcaga
    17521 gagggacagc ttgtttacag actctcgcag ctaaccggaa aaaccaccca atctatctat
    17581 gcgtcatatg aagatgccgg cgccacctgg tatgtgcctg tgggcgaggt gtcggcagat
    17641 gagatcggaa atcaattcaa caccctgctg gaaatgggtg ggctggtgtt atcggaattt
    17701 acgtctcgat attattacgg cgggggaatc gcccctcaag ccatcgggta tgtgctgggc
    17761 atgtcagaag aacagcaagc cgagtatatc caaaaggggt atttggggga tgagagcgtg
    17821 gggcaggcag gattggagaa atatgctgaa gaagcgctgg caggaaaacc atctgcatcg
    17881 ctttacgtgg tcaaccctga tgggcaggtt gtcacgcgca tatcgcaggc agacccgacc
    17941 ccagcccaat cgatcaccac gacattcgat aaaaatctgc aaattgaaac ccaaaaagcc
    18001 atcgcaggat ttaaaggagc agcaatcgtg atggaggtgg atagcggacg gatcctcgcc
    18061 atggcatctt cacctaactt tgatcctaac ctcttcaatc cgagcaatat caacagcgct
    18121 gaactgctga gtggagtgtt gaacgataaa aatcaaagcc tcctgaatcg cgccacgcag
    18181 ggattgtacc ccttgggatc tgttttcaag atcgtcacca tggcagccgc attagagagc
    18241 gatctgtata ccgctgattc atcctattat tgcagttatt actttgagga actgccgggt
    18301 gaacgattcg aggattggac cctggataaa gggtatgacg aaagcggcga attgaccctg
    18361 gtggaggggt tgatgcgctc atgcaaccca tggttctatc acctgggttt agacctgttc
    18421 cggcagatgg gcgccaccta tctatctgat atggcacgcg gatttggatt gggcagcgca
    18481 accggcatcg agcaggtggc agaagatatc ggttccattc ctgaccccaa aaccgatgga
    18541 aatgcggtgc aacagggcat cggtcaggga gaaatgttgg tcacaccgct tcaggtggtg
    18601 cgctttatcg cagccatcgc caatggcggc acgctttacc gcccacaact gatcgaaagt
    18661 atgaccagtt ccaccggcgt gacgacgtac actttttcac ctgaagagca gggaaccttg
    18721 cccatcagcc aaaccaccct ggaaacgatc agagagggaa tggggctggt tataagttcc
    18781 agacgtggaa ccgctcacga cgcgttgagc gggctgaaca tcaccatgta cggaaagacc
    18841 gggacagcta cgaactcaac cggcgatccg cacgcctggt tcgctggata cacggatacc
    18901 aaccgggaag ataagccgga tatcgctgtt gtggtcttga tcgaaaacgg tggggagggt
    18961 tctgaagtgg ctgcgccggt tttccgccgc atcatcgaaa cgtattattt cggtgaaccg
    19021 ttgaagctat atccatggga atccgagttc aatgttacca gaactccgac gttggaattc
    19081 acgctaactc caacgcaaac cccttatcgc gaaccggtca agatcgaacc gactgaaacg
    19141 ccggctggat gagcgagaca cgtgcagttg gtatggtaac ccgccttcag gcaggatttt
    19201 atactgtcag gatggagaac tccacgctga cctgtcagtt gcgcggtcgt ttgaaacagc
    19261 acaaaaaaat ggaggacctg gttgccctgg gtgaccgggt tcatgtcagc gtcatcgatg
    19321 aggagcaagg ggttattgaa gaggtcggtg agcgcgagcg cgcgctggtt cgcatggcgc
    19381 cgaccacacg cgggatctac aaacagattc tgctggcaaa tcccgatcaa accgtgtttg
    19441 tgttcgcctg cgctgatcct gatcccagtg tacgcatgct ggatcgcttt ttggtgatcg
    19501 ccgaaaagca aaaaattaaa gcattgatcg ttgccaacaa agtggaccgc ttggatcgca
    19561 agcgcgctga agagatcttc tcaccctatg cccatttagg ttacacggtt gtttatacgt
    19621 ctgcaaccga gggcagcggg atcagcgagc tgcgcgatca cttgcagggc aagctgtcgg
    19681 cgtttgcagg accttcgggc gtgggcaaaa cgagcctgtt aaatgctctg cgacccggac
    19741 ttggtttgga agttcgatcg gtaaaagaga gctcgggcaa aggcaggcat accaccatcg
    19801 cgcgccagct tttttcgctc gacgaatcca gccaggtcgc tgatctaccc ggcatcaggc
    19861 agctttctgt gtgggatatc gaaccggaag agctggatgg ttactttgtt gaatttcccc
    19921 cgcacgtgaa gaattgccag ttcagcgatt gcagccacaa ccgggaaccc ggttgcgggg
    19981 tgaggcgagc ggtggacgat gggatgatca gcactgcccg ttatgattct tacctgaagt
    20041 tgagacatga actggcagca caacgtactt attgaacatt cataggatag agaaacccgt
    20101 atgaaaaaca aaccaatccg cttgttaatt tttttcgcca ccatgatcgc tatgctcagc
    20161 gctgcctgtt ccgtacaggg cttgtcgaaa gcaaccccga cagcgcctga acccacagca
    20221 cccccattaa aggaaattac catttgcact gcagaagagc catcgaccct gttctactat
    20281 ggagaaacga ataggggttc tgacctgatc tttgaagcca tctatgacgg tccctttgat
    20341 cgggttgatt atttggcgca agaagtgatg atggataaag tacccaacat tgaagatggc
    20401 agcgccagtt attctccggt tgaggcgaaa agcggcgatc tgattgtgga tgtgcacgga
    20461 aatattgtca atctggtgga aggcgtgcag atctttccca gcgggtgcca aagcatggac
    20521 tgtgccgcta cttgggatgg gataagccag gtgctgcttg accaactgat tgtacattat
    20581 tcggtcaagg acggcgttag atggtcggat ggcgaaccgg ttaaggcatc ggattctttg
    20641 ttctcgtatc aactggtggg agagctacaa aattcgaaat atgaggatgt gcttggcaaa
    20701 atcgtcgact accaggttgt tgatgcgcag accgtgaaaa tgagcacatt gccgggcgtg
    20761 gtcacttctg ataccacagc attctttttc tccccgttgc cacagcacaa cctggaatcc
    20821 tacgcagcgg gtgaccttgc aacaacggat gccgctgcca gatatccaat ggggtgggga
    20881 ccatttaagg tcactgactg gcagccgggc gtgagcatca cgctggaaaa gaacgaattc
    20941 tatttccgct ccgctgaagg atacccggca ctggataaga tcatagtccg gtttctggag
    21001 cggggggagt ccatgcaaga aatgatcagc agccgcgcat gcgatgtggt ggatgattct
    21061 ctcatcacga aagatggctc tactgattta agcgtactgc aagacgacgc aacggttcgc
    21121 gtcagtatcc tgccgggaga aaattggaag gtgttgttgt ttggaattga accagccagt
    21181 tatgatgacg gatattaccc gtatggttcc gatcgccctg atttgttgag tgatgccgcg
    21241 tttcgtaacg ccatccgtca gtgcatcgat cagagcgcaa ttgttgcaca gcagagtgtg
    21301 gtgggaaacg atcagccctt cgtttatttc ccatatgata cccagcttac agccgatgcc
    21361 agcacctctg ctgcatatga cccgttgagt gcaggcgcgg ttctggatgg cattggatgg
    21421 agagattatg atgcgaaccc ggctacgccg cgtgtggcgt atggggtggc taatgttccg
    21481 gacggcactg ttctttctct gaacctgtat accaccacca gcacagacaa catgcagatc
    21541 gcgggggaca tcgcgtcatc cctgactgct tgtggaatcc aaaccacagt ttatcccacc
    21601 cctgcaacag agctttatgc agaaggaccg gatgggatcc tattcgggcg aaaattcgac
    21661 ctcgctttga tcacatggaa tacaggcgat gaactgcctt gcgctctatt tgagagcgac
    21721 gaaattccag cggaagacaa ttattggatc ggcgcgaatg atggcggagg gaacatcggc
    21781 gggtatcaaa atgccgaatt cgattcacta tgtgctgcag cccgctttgg cagcactgac
    21841 ccccaagccg cactgagggc tcaggttgaa gcagcaacga tattggatga ggaaaccccg
    21901 ctcatttctt tgttcttcga tgcgaaagtc tatctcatga gcccacaact ttgcctgcct
    21961 gagaaggtga acaatcaaaa atatccctat tcttcgctgg agtattggga ttttcgtgac
    22021 acttgtaatt agatgtataa tggaaaaaca gttacaataa caagatattg gctatgttat
    22081 gcaaatctat gtaggacagt aaacagttga catgccattt taagtattgt aaaatagtcc
    22141 gaactttcag ccaggtccgg cagatttttg gtatctgttg gcaaaattct tcagtaaagg
    22201 gcattatcaa tggcagaatt attcagtgta aaagacctgg aaacgcagtt tcacactagg
    22261 gaagggacag ttcacgccgt caacggagtt tcactctctc tgcaagaagg tgaaacgctc
    22321 ggtattgtgg gggaaagcgg gtgcggaaaa agcgtctcca tgttatccac gttaagattg
    22381 atcccccaac ccccagggga gattgttgcg gggaaagcca attttcaagg aagagattta
    22441 ctagaactat cgcgcgaaga actgcgccac gtgcgtggag cacagatcgg gtttgttttc
    22501 caggatccga tgacttcatt taatccggta caaaccatcg gaaagcagtt gatggagcca
    22561 ctggagatcc acctgggcat gaataagaaa caggcctggg accgcgcgat agaaatgctg
    22621 aacatggtgc gcattccgaa tccggaagac cgcctgaaag attatccgca ccaattctcg
    22681 ggtggcatgc gccagcgcgc catgattgcc atggcactgg cttgctcccc ccaggtgctg
    22741 atcgccgacg agccaacgac cgccttggat gtgacgattc aggcgcagat catggatctg
    22801 gttctcagtt tgcgcaaaga actgggcatg tccatcatct ggattaccca tgacttgggg
    22861 gtgattgccg ggttggctga ccgggtgatc gtgatgtatg ccggttatat tgtggaagaa
    22921 gcaaaagtaa aagacctgta tgttcgcccg gaacacccct acacgttagg gttgctgcgc
    22981 agtcttccgc gcctggatat ggatgaaaag aagcgacttg tttccattga aggtgtacca
    23041 ccggttctct tcaaaaagcc aaaattctgc ccattcgcat cacgctgccc ataccgcgtt
    23101 gagaaatgtc tggatgaaaa cccaccacta atgttggtgg gggagaacca taaaaccgcc
    23161 tgctgggttg acacctatac tgggaaggag cgctaatgcc agaacgagaa gttatcttgc
    23221 atgttgaaga tttgaaaatg cattttccga tcaaacgggg tgtaatccag agaaccgttg
    23281 ggtatgtgca tgctgtggat gggatcacat ttgatatcta tcaaggtgaa acgctgggtt
    23341 tggtaggaga atcgggttgt ggaaaaacca ctgctggcag aaccattcta cagctttaca
    23401 aagcgacctc agggagcgtc attttccagg ggaaagacct ggtgcaactg aaaagtgaag
    23461 agatgcgcca gacccgtcga aagatccaaa tgatctttca ggatccttac gccagtttga
    23521 acccgcgcat gactgttgaa gagatcatcg gagaaccctt gctggttcat aatattgaaa
    23581 acgggaaaga gatccgtgag cacgttaagg atctgctgga attggtaggc ttgaaccccg
    23641 cttatgtaga ccgatatccg catgaattct ccggagggca acgccaacgc attggagtgg
    23701 cgcgctcgct tgcattaaac ccagatctga tcgtgtgtga tgaaccaatt tctgcgctgg
    23761 acgtatccat tcaggcacag gtcgtgaacc tgttggagga cctgcaaaga gaattgggac
    23821 taacctatct tttcattgct catgatctat cgatggtacg ccacatcagc agtcgcgtta
    23881 tcgttatgta cctgggtatt ctggtggaaa tggctgatcg caatacgctt tataacaacc
    23941 ctatgcaccc ctattccatc gcgttattat cagcggttcc gatccccgat ccgaaagtag
    24001 aggaaaagcg gaagcgcatc attttggaag gggatgtacc atccccgatc aacccacctt
    24061 ctggctgtcg atttcgcacg cgctgcccgt tggcacaagc ggtttgcgcc gaggaacgcc
    24121 cggagtggaa agaagtggaa gcgggtcact atgtggcttg tcatttctgg tataacacaa
    24181 aatctttaca aaggaggtga tggccaggag agaaaacttg ttgtttcagc agttcgttgt
    24241 gtttcaaaag tcaaaaaatt tatggaggag caagatgcgt tcaaaatggt atgttttatt
    24301 tagtgcatta gtgatccttg cgacactcct cgttgcctgc caacccgcag cggaagaagc
    24361 tgcaccggga gcagtcgagg aagcggcaga tgctgcgtca ttctggcgaa cgtccaagga
    24421 tcctacaacc tgggtagaga caacatttgg cgatcctgag actctcgata cctcccgcga
    24481 ttatgaaacc ggtggtggtg agattctcgc aaatgtttat gacactcttg ttttcatgga
    24541 aaaagaccag gcttcaactt tcgttcccat gctggcaacg gaagttccct cgcttgaaaa
    24601 cggtgggatc tctgaagacg gtttgaccgt taccttcaag atccgcgaag gcgtaaagtt
    24661 ccatgacggt tccgatttga ccgtcgatga tgttgccttc agcttcgtgc gccgcattct
    24721 ggctgggggc acttcgtctc cgcaatggat gctggttgaa ccattgttag gtgccggact
    24781 tgtggacatc gcagaaattg ttgatgccga gaatccaccc tatgatgatc aggcaaccct
    24841 ggcagcctat gatgccgaga ccctcgcttc tgtttgcgaa ctcgtggaaa ccaaggtcgt
    24901 tgccgaccct gccgctggaa ccgttacctt caaccttgca caaccgtggg ctcccttcat
    24961 cgccaccctg gcgaatggtg gttgggctac tatcgagagc aaggcatggg taggcgccaa
    25021 tggtggttgg gatggcgact gcgcaacctg gcagaattac tattcagttc ccaccgaaga
    25081 gctgaatcag cttggtattg gtaacagcgc catgggaacc ggtccttaca aattcgatca
    25141 ctggacaccc ggtgaggaaa tcgttttgac cgccaatgag gactactgga tgaccgaacc
    25201 catgtgggaa ggcgccccaa ccggcgctcc tgcgatcaag accgttgtga tcaaacaggt
    25261 tgctgaattc agcacccgcc tggcgatggc gcaggctgga gatgccgaca acatcctcgt
    25321 cggttcaaca gaagattggc cgatcctgga tgaaatgatc ggtggcgaga tcacctatga
    25381 agaatacctg gctggagaac cagtcgtaat caccgatccc accaaaccat tctacaaagt
    25441 ttccgaaatt cttttcgcca ataatcgtac cgacattggt ttcaacttca acgtcaatgt
    25501 cgaaggtgga aactccttca ttggtagtgg tgtattggat ggcgatggaa ttccggcaga
    25561 tttcttctcc gatgtgcacg tccgacgcgc cttcagctac tgcttcgact atgagacctt
    25621 agcggcggaa gtcatgcagg gcgaagccaa ccgcgctcca gttcttatgc tgcccggtat
    25681 ggtcggttac gatgaagatg ctgaacacta cacttatgac gtcgaaaagt gtgtggaaga
    25741 attgcaagcc tccacctggg tatcagaaga tggcgtacag ctctatgatc ttgggttccg
    25801 catgtccgca gtgtacaacg tgggcaacac cttgcgccag accattgccg aattgctgca
    25861 ggctggtttg caagaagctg gcgagcaatt tgtggtcgag gttgtaggtt tgccgtggcc
    25921 taccttcctg gcgaatatta acgccaagaa gatccccatc ttcatcatcg gctggctgtc
    25981 tgactacttc gacacccaca actggacttc aaccttcacc tctgcctact attcattcag
    26041 gcagaacttc ccggctgaac tgcgagcaca gttctccgag atcaactcac gcgcagtaca
    26101 ggagaacgat cctgcagaac gcgagagaat ctacaaggaa gaattcaaca ccctgtattt
    26161 cgaaacagcc ccagctatgt tgctctacac gctcaaggga cgtcactatg cacaaccttg
    26221 ggaaaaaggc tggtactcta acccgatcta ttccaacaag tggtactacg ctctgtcgaa
    26281 agactaagat cgagttaaaa aaagcgctga gggtagggta ccctaccctc agcaaaactg
    26341 aacgaaacga gaagaaaaaa atgacccaat atattgttcg tagattgctg ctcatgcccc
    26401 tgaccttgtt aggagtgacc cttctcattt ttggaatgct ctcattctta tccccggctg
    26461 aacgggtttc tttgtacgtg agagatattc ccaagaatga cattgtgatg aattccctca
    26521 tcgagagata tggtcttgat gatccattct tggtgcagta ctaccactgg ttggtcggtc
    26581 agaaaaacga agtgacaggc gaatgggctg gaggaatact gagaggaaac ttcggatact
    26641 cccgctctgc gaatcaaaat attgttgatc tgatcaagcg gcgtttccct gccacggttg
    26701 aactggcgct ttattccatt gttcccctga ttggggttgg aacctggctg ggaatcgtag
    26761 cagcaaaaaa ccatgacaag ctcattgacc aaatagcgcg cctatttggc atattgggtt
    26821 attcttttcc atcattcgta tttggccttc tcatgctgat gttcttttat gcgaagcatc
    26881 aaatttttcc cccggatcgc ttatcgaact gggcaatgct gatcgtctcg ggcgaaggat
    26941 tcaccaacta taccagcctg gtgacgattg acgctttact gaacggtcgc tttgatattt
    27001 ttctggacgc gttacggcac atggtattgc ccattcttac actgtcctat atcaactggg
    27061 ctctctattt gcgcatcatg cgatcatcca tgcttgaatc catgaaccag gattacgttg
    27121 taaccgcgcg tgcaaaaggt gtgaaagaaa aagatgtgat caacaagcat gccagaccta
    27181 atgcgatgat cccaattgtc accatgagcg gtatgaccat cgcgggtctg ctgagcggtg
    27241 tcaccattac cgaaacgatc tttatttatc ctggaattgg ttctgcagcc gcggctgcgt
    27301 cgacccagct agatgtggtc acgatactct cgctggtgtt actgacaggg ttcatccttt
    27361 tgattgcaaa tctgatcgta gatatcacct atgccttctt agatccgcgg gtgcggttat
    27421 cttaggctgg gcgaaggaga gttttgaatg gctgaagcac aggtacttga caagaaactt
    27481 gaaggattga tcggtgaata taccgaacgg aaaatctccc gtgcagaaag gatcctcgga
    27541 cccgagtttt accggatatt gaagggaatt tttactaccc catcttctat tgttggattt
    27601 acattaattg ccatcttcgt tctcgtggcg atatttgccc cgcagatcat tccaccaaaa
    27661 gagaacaccc tggatccata taaaatcatg cgcgatggat ttggttccat tcccaaagcc
    27721 ccaggcgcgg tgtgggtaaa aaatccgcca accgttccgt ggtggtatcg gacgtttgtg
    27781 ggcgatgaat gggttcatct gttcggcacc gccagcggtg gatgggatat cttttatggt
    27841 gtgatctggg ggacgcgcac tgccatcaaa gtgggcatca tcattgaagg aatcacactt
    27901 attctgggga tcattattgg ctgtctggct ggtttctacg gcggtttcct tgatacgatc
    27961 atcatgcgcg tcgttgaggt gtttcaaacc ttcccgttta tcatggctgc catgacgctc
    28021 tctgccattc tgacacccat catcgggaaa ggcatttggc cagcaaccat tgcattggtg
    28081 gtttttggat ggatgagcta tgcgcgcttg atccgcggcg atattttatc ggtgcgcgag
    28141 cgagattaca ttttagcagc gcgcgtggtg ggagcaaaag acagccgcct catgttcaag
    28201 cacatcattc cgaacgccat ttatcccacc ctggtggtag cctcgctgga tattggaacg
    28261 atcgtggtgt cgtttgcagc cctgagcttt ttgggtatcg gcactgagat cggttacgcc
    28321 gactggggac aactggtcag tttcgccaga aactggattc cacaactggc taactactgg
    28381 tggattttga tctatccggg aatggcactg gttttgtttg tactgggttg gaacctggtg
    28441 ggcgacgcat tgcgggatat cttggatcca agaatgagaa acaggtaatc aatcgtaaaa
    28501 tgacagcagg gtttcgtgaa agcggaaccc tgtattttat taaaactcaa agtatgttaa
    28561 aatatcttcc tcttgaaggg aacccattaa tggggcgtgg tgcaatggca gcacagcaga
    28621 ctttgaatct gtttatcttg gttcgaatcc gagcgcccca gcttgagaaa agatgaagag
    28681 aaaagaaacg cgagcaaact gatgaaaggt tggctcgcgt tttgttaaca tagaatggag
    28741 tgaacaatgc gcattggatc agtgattctg gcagctgggg tcagtaaaag gatgaattca
    28801 gctatcccca aagtgatcca tccacttatg ggaaaacctt tggtgtggta tgcgattgaa
    28861 gctgccaaag cggtggcgag cgtgccgcct gtggtggtaa tcggatttgc tggggataag
    28921 gtaaaagaaa ccatcaaaga aacagtttct tacgccgaac aggcggagca gttgggaaca
    28981 gcccacgcag tgaggatggc agaagaccaa ctgaaaggga aggtcgacct ggtggtgatc
    29041 acatcagcgg atatgccgct ggtagcggct gaaaccctgc aaaggttggt cgacatccaa
    29101 tccaggagtg ggggagcgat gaccctggtc agcatccagg gggacgaatc aagagggttt
    29161 ggcaggatcg tacgggatgc ttcggggaat gcggtcgcca ttatcgaaga gaaggttgcc
    29221 tcagaagagc agcttgcgat caaggagtat aacgcaggaa tttactgtgt acaaagcgat
    29281 tggttgtggg acgcactgcg gcgggtaaaa aaatcgacgg tcggtgaatt tttcttaact
    29341 gacctgctgg agatcgccgt tcgtgacgag ctgccggtga ccgttctgca attggataac
    29401 gttgaagaag ccattgggat caacaatcgc gttcatttcg ctgaagtgga aaaactcatg
    29461 cgtcgacgca tcaatgaaca acacatgctc tccggtgtca ccatgatcga tccggagcgc
    29521 gtatatatcg aaccttcggt gcagatcggt agagacacgc tcatttttcc tgaaacctat
    29581 ctgcgcgggg aaacgcacat tggagaaaat tgtgaaatag gtcctggtac gatcatcgac
    29641 aattctttga tcggaaatga ttgcaaaata ttgaaaagcg ttctcgaagg agctgaattg
    29701 gaagaccatg tggaaatggg tccattctgc cgattgcgta ccggtgctca tctggcaagc
    29761 catgttcaca tgggcaattt tggcgaagtg aaaaaatctt atcttggaaa aggtaccaag
    29821 atggggcatt tttcgtacat aggcgatgcc cagatcggcg agaacgtgaa cattggggcg
    29881 ggtacgatca cctgcaatta tgacggaaaa aacaagaaca agaccgtgat tggtgacaac
    29941 gcgtttatag gcagcgatac catgttggtg gcgccgatca cgatcgggaa aaatgccatc
    30001 acaggggcgg gtgcggttgt tacgcgggat gtcaatgatg gcgagaaagt ggtgggggtt
    30061 ccagctcgtg tgatcaagaa cagaaaagac gaggaaacaa atcatgattc aatctgacat
    30121 tgaccaaaaa accaacctga tcgaagcagc ggtcgaagcc cggaaaaaag cctatgttcc
    30181 ttattcgcgc tatccggtgg gggcggctct gttgacgaaa gcagggaaga tctacacagg
    30241 cgcgaacatt gaaaatgcgg cttttccggt aacgatttgc gccgaacgga cggcgatctt
    30301 caaagctgtt tcggagggag aaagggaatt gcaggcaatc gctgtggtta ccaagaatgg
    30361 cggcacccct tgcggctcct gcaggcaggt gatggcggaa ttcaaccctg atctgattat
    30421 ctatatcgca aatgagaagg gcgagatcgt gcaggaaaca accttgaaag atatcttgcc
    30481 cggttatttt ggtccgaaaa gcctggtgaa gtaaaaacag aatggaatga gtgataccga
    30541 gcggttagtc aattctgaag cctatgtgat cacgcaaacg gattacggtg aagccgaccg
    30601 tttttacacg attttttctc gtaagagtgg aaaaataaaa gcgctggctc acggtgcgcg
    30661 caaagcaaaa tcacgcaaag ctgggcatct tcaaccgctt acgctgattc acctgatgct
    30721 tgccaagggc aagacctttt gggtgatcac gcaagccgat acgctccaga cttactcttc
    30781 cataaaaaat gatctgggga aaaccgccag cgcactatat atttttgaac tgatcaaccg
    30841 ttttgctcca gaaagcgaac ctgtccatcc attattccag ttagccaaag acacaatgga
    30901 gcgaatcgaa aaagaacagg atcgtttttt tgcaatcaaa ttctttgaat ttcgcttgtt
    30961 gaaacatacc ggatatatgc cgaacctgac gacctgcgtg cagtgcggtg aagcgatact
    31021 cccacaagat caatatttct cggcagaaca ggggggcatt ctgtgcccgc gctgtggtct
    31081 gcgaggatac acggtgcgga aggtcagctt accagcgttg aaatatctgc gtttcattca
    31141 gcgcagcagc tactcggagc ttggtaaagc caaactttcc acggaagcaa aagaggaaat
    31201 ggagaattta atgctgtatt atttaacgta cataacggaa aaagcattga acacgccaac
    31261 cttcatccat caggtcgaga gagcggaaaa agagatggag caatgatgaa cacagaaaag
    31321 aagaactttt caaaggaaga gcaaatacgg ggtttgatcg aacagattga tgcctatatc
    31381 gatcattatc atggtggttc tgtcgaattc ctatcattga aaggaaatgt tcttacggtg
    31441 cgcttgggtg gagcatgtgc ggactgttct ctgcagcccg cgacattaaa aggatgggtg
    31501 gaaggcaccg taaaacaatt ctttcctgag gttaccgaag ttttatcaga aaaataacgc
    31561 ctctggaggt tttaccattc tttcagaaca tacagcacgg atgtgtcagc ccgtcatcga
    31621 gatcgtccgg gctgacgtgc agaaagcctg ccctacccga cttgttggca ggccagcacc
    31681 ccacctcctg ttttcccgca agcagggagt cgtgatgggt tagaaaccca tcaggatgac
    31741 gttgctacga taaattctgg aagaagaatg gaaatggaga gatttctcgg tcgcgctgct
    31801 ctctcgaaat gacattgcgg gggaaaatgg gaaaaagcaa gttgactggt ttagtgggta
    31861 aatggagaga tcctttcaat cctgcttttt gtattgctac ctcttgatat ttaagacggt
    31921 atctacaatt tgtgacaggg atggttggta gctgagctgc tgtgatctgc atccttgcac
    31981 gctatcaacg ccaggaagat gccgatctgc atcatcgact ggactggcgg ttcctattca
    32041 gccgcaggaa tctgcgtggc tgaataacga tcgatcgcag acgatcactc caacagccag
    32101 aaaagaatca ccctccagtg atgccttttt tattttgatc tttttggtaa aattgttctt
    32161 ctatggagca gccgtggcgg attgggtatt gacgctgatc atcgcagtaa gtttggcgat
    32221 ggattgcttt gcggtttcat taggcgtggg atgttctctg ccttcctgtg ataagcgcgc
    32281 gatctttcgc atgtccttcc attttggctt attccaggct ggcatgaccc tgttcggttg
    32341 gttaatagga aataccatct tgccgtatat tgcgcaattc gatcactgga tcgccttcct
    32401 tttgctggcc tggattggaa ttcgcatgat gcgggaagga ttatcaaacg aaagcgaaaa
    32461 aatgaccgga gatccaaccc gaggcagcac gctggttctg ctttcggtgg cgaccagcat
    32521 tgatgctttg ggagtggggt tgagcctatc gatcctgcag acgaatatcg ctttctcaag
    32581 cgcgatgata ggcatcattt cactcttgtt cacgatcatc ggattgaaat ttggcaatgg
    32641 cttgaatcat aaattcggaa aaaggatcga gatcttaggc ggattggtgc tgctattcat
    32701 aggatcgcgt ttgctcctat cgcatctaag cggataggaa atttctgtaa caatgcagat
    32761 tttccacgac ttgacatttt tctgaattca gttgacaatt tctgctgctt ctccttcctt
    32821 tataattaga gaaggagatt ggaggtacat aatgaaaaga atgaaagtca tggttagttt
    32881 cttgatcatg gcagtggtga tcctatctgc ctgcacccaa gcggctgcat cagaagctac
    32941 accggaccta ggtgccgtga aaacgcaagc tgtccagact gcggtgagcc agatgaccgc
    33001 ggatgctgag agcggcaaag aagctcccgc atcaacatct ccgacgatca cacccctaac
    33061 gaccatcagc gctgtagttc tgccaacagc aaccacccgc gtaagctaca gcagtggtag
    33121 cggcggtagc agtggaggaa gcagcagtgg tacttcatcc ggcacaccca ttcccaccgc
    33181 aacccctgat gtgtatattt gtgaagtgat tgggcaaacc ccgctggatg gcaaccagta
    33241 cgtaggcgct gatattgacg tcatctggac gttaaagaat gttggtgtgg caacctggac
    33301 agccggaacc tattattatc attggaccgg gtactctgat cttagccccg tccattcttt
    33361 cacccttacc caagacgtac cacgatacag taccgtccag gttatgatcg acgtgagggt
    33421 accgattatc cccggacagt atcgaacgca gtggtatatg gtcaatgaca atggtgagaa
    33481 tttctgcggt ttctattatt atgtgaacgc gatcccgctg ccaacagcga cacccgatta
    33541 ggtaatgcaa aagagaggtt ccataatcct ctctttttgt ttccgttgat gcttatgcaa
    33601 acacaaaata gatgagcgca caatagaccg aatagggaaa aatccagatc aaaggtttta
    33661 gtgcatgtcg cagcggcttt ttctttcctt gttctacaac cgggttctgc tcaaagatcc
    33721 accgtatcac gagaaagacc gaactataaa gaatggcaaa ctgccaataa ccaaaaatga
    33781 caagatcgct cactatatag aagatctcca tgctttgacc agataataac ggaaccagaa
    33841 atccgttcaa ccagatttcc atgatcagtt cgtacaaacc actcagcaga atcagaactg
    33901 cccaggaaaa attgtatttc tgttggatgg ggatgaagat caggatgatg ccaaccaacc
    33961 agggaataga gaggagaagg ttaggactgc caactgcgct ctcggtattg atgcttgatt
    34021 ggatcaattg agcgcagaat ttgatcatga aactgatgaa taaggcgttg gcgatggcga
    34081 agattttcgc attgctcact gccttttcgc tcgaaactgc gggagcccag atttttcgac
    34141 tgaggtatag cagcaaggta taaataccgc tgaaaacgat cgccatcacc atggttaaat
    34201 tctccgctgt tggattgatg gcggctccaa atatggaaac tcccagaccg tataccagac
    34261 cgatgatcac aaagatctgc aaccatggtg gggattttct ttgcgtggga ggattttcat
    34321 ctggcagggg tgggaggagg gcggctggtt gttctctttc aacgcttttc atgtttctca
    34381 agctctttca ttaaatttcg gcttctggat cggacagctt tcgactggtt ctccatgccc
    34441 gattccagat aacttcttgt gctggcaagc aaccgcgcat cacggaggga taaatcaaat
    34501 aaacattgca agccgcatgt ttgaacgatg gcacttgaac tggaaaggta tttttccatc
    34561 gtttggtatg cgcgagaaat ttcgtcgttg gtgagcggca agcgaggaat gatctgtgca
    34621 aggtgccatt gaacttcgat ctgctcggcg ctttcaagca actccagcat tggctcttta
    34681 taaggaatca acaatgccgg gtcttgcgca gtgacctttt cgaccgcatc cgccgttcgc
    34741 atgcggatta cgggatcggt ttcatagaat ccgcgaaaaa gatcgtcgaa cttatcgggt
    34801 tgttgcaaga tgagttcaac cacctcatca gatctgccga tggagcgtcg gtctccccca
    34861 ctcaacagct tcaagatttg attcatggga acaaaaattc cttttctccg ttatgattat
    34921 aaaaggaatc ggaggagaaa tggctctcag aaaattaagc gttttgttcc ttgcagtgtg
    34981 catagctctt tttgcgggat gctcacaagt tccgaagtca aaaccggatg atttcacctt
    35041 gtattactac tggaataccg gttcgttgcc gccggaatat tattatcaat atgagatcga
    35101 gattgatcca ggcggtaatg ggatcttcac acttcagcgg ggatatgaag aggtagaaga
    35161 aaatagaaaa gcattttcct tcgtggttcc tgcccaagat tgggagaatt tcttcgtctg
    35221 gctcacagag aacaaaatct tgcgcaataa ttggagagaa acggcagata ttttagtagg
    35281 agcaagcaca acagaggcaa gaattcaggt caatggcgaa aaatatacca tcccgagcgt
    35341 gtccgttctt tctcaaaggg ataaaggcac cttctacaaa ttagaaggac aaatcagacg
    35401 actcgttccc aatgagattt ggaaccaggt tcaataaaac atgtggtatg agtgcacaga
    35461 actctggaac ccggacttgt ttcagatgga aatacggtag aagatcatag actttacaaa
    35521 tgtgcttgac atatgtgatt ctcatgctat agtaagggca tgcagaagaa catatcactt
    35581 gtccgaatag acgatcgttt gatccacggt caggtaacca ctgcctggct tcgcgtttac
    35641 cccgccgatg aaataatcat ctccaacgaa aagatcgcca atgaccccat atataaggcg
    35701 atcttttcag ttgcgcagat ccccggaaaa gaaattctgt tgctttcgcc ggatgacacg
    35761 gtggaacgga tcaatgcgat gaagcctggg tgcaaatatc tcattattac ccccacaccg
    35821 gtggatattg tcacgctgat cgataaggga ttacagatcg acaagatcaa cattggtggt
    35881 ttacagaaca gaccgggtac aacccgtttg gcaaaagttg tgtatgcaaa agatgatgaa
    35941 attgcagcat ttaaagcgtt gcaaactcgt ggagtagaaa tggaggtgca gatggttcca
    36001 actgaaaaaa tcacaaaact gaatctgtaa aacttttgtt gaaaaggaga aaattttatg
    36061 tggaaagaag ctactctcgt tgcgattgtt gccggtggct gggcgcttgt cttgtattat
    36121 cttccccaac tcatcacagt gctttctaaa aaagaaagca aggcgatgac ctggtggtta
    36181 ttgttaatgc gtggagtgaa tgatgcattt agctacgccg tggtgattcc ggcagcgtta
    36241 actggttggg tgcttggtga tttaaggacc ggtttgatcg tgggtggtac tgttcaggct
    36301 atgttcatcg gtgtgttcat tgtgggcgct tccattccgc ccaatcccta tctggcatcc
    36361 attttgtcga ccgcgctggt tattttgacg ggtgcatcca ccggtgaagc gttagccctg
    36421 gttgttccca tcgctgtggt aagccagttg ttgacattgg cattcatgag tttgaatgtt
    36481 gttttgtgcc actggggaga caaaatggcg gcggatggaa atgcaaaagg aattgatatc
    36541 ctcaacaccc tggatggtac tggttggacg ctggtcaatg tgattcctgc tttcctagga
    36601 gtggggctgg gaattgatgc ggctgcaaaa ttgctggata tcatccctgg ttggttggcg
    36661 gatggtttga gttatgctgg ggggatcttg ccggctctgg gtattgccat gctgttattg
    36721 atcatcgcat ccaaagaagt atggccgttc ttcttcctgg gtttcattgc agcggtttac
    36781 ttgaatttga acgccgttgc gggagcgata ctgggtgtgg taattgccat catcttcgtg
    36841 cagcttaaat ctgcaaaaga agaaggaacg aaagagaagg gaatataaca tggacgccaa
    36901 aatgaaaaag ttagatgaga agagtcttta tggcgctcta tggcgcgtta acaacttcgc
    36961 cggcaactac aacttcgaac gcttgatggc gttgggcatg acccaggtta tggttcccgt
    37021 attccgcggt ttgggaatca ccggcgacaa attgaaagag ggtttaaagc gccatatggt
    37081 cttttataac acccaccctt attttggtgc gttgatcatg ggtgtgatgg ttgccatgga
    37141 agaagccaag gcgaatgatg atgcaggtgt aacagaggaa gccattaatg gcttcaaagt
    37201 cgcattgatg ggtccttttg ctggtatcgg cgactcattt ttctggggaa ctgtgcatcc
    37261 catcattctg gcagtttccg cgaacctggc attacaagga aacttcctgg gatttttgtt
    37321 cgcttttctg attgcggctg aagccaccct gggaactttt taccctttca tttggggtta
    37381 caagagtggt acggaaattg tagaaaagat ccgcaccagc aaactgatcc aaaagttcac
    37441 tgaaggcgcc aagatcgtgg ctatgtttgt tgcgggcgtt atggtgacaa cgttgatcag
    37501 tatttctacg cctgttgttc tgttcgccag cgaagcagaa ggcagcggcg tgaatctgca
    37561 gggcatcttt gattccatcc ttccaaagtt gttaccgctt ggattgctgt tctttgtcta
    37621 ttggttatta aagaagaaaa tcaatccatt atgggtaatg cttatcttga tggtgctcac
    37681 ggttggtctc aaagccctcg gatgggtagg ataaaaaata ccggtttata tagatagtag
    37741 gtgggggagc cagggattcg cccggctccc cctagatgtg gaggtcgtat gagagcagca
    37801 gtatacacag agccgcgcaa gatcgtgctg gatgcgaatt accctgatcc ggagatgggt
    37861 gatggggtga tgatccgcgt gcgtgcaagc ggtgtatgtg caactgacat taaagcattc
    37921 aaagggctgc gatttggaat ggaacccccg atgatattgg ggcatgagtt cgccggtgaa
    37981 gtgcttgttt cgagcatgga tgattataaa attggggagc gcgtttccgc tgccccttat
    38041 gcaggttgtg gcttctgcaa ttattgtctg gatgatcatg aagaattgtg ccaagacaag
    38101 gatttcacca caaatggttg ctttgcagag aaattgttca ttccgcgtga tttaatgtat
    38161 aagacaggtt ggaaaatcaa gtccgacgtc cattaccagg cagccgcatt ggctgaaccg
    38221 ctggcatgcg tcatcctttc attagaatcc tgccattggc agccgggctg gacgatcatg
    38281 gtggtgggtg gtggctttat ggggatcttg cacgtcattt tggcaaaagc ctggggagcg
    38341 accaagatcc ttctcagtga gccgaatgcc aaacgccgtg cttttgctga aaagcttggt
    38401 gcaatcgtgt gtgatccttc tgaagaggag agccttggcg attttgcacg aaaaaacgtc
    38461 gatggtctcg gacctcatgt ggtcattgcg tccattgcca atgagcaagt tgttgaatca
    38521 gcttttgatt gtccgcgtgc gggtggctat tatcatatgt ttggggggct tcccaaacaa
    38581 agcaccatca ccatcccctc ttttctcacg cattataaag aggtggctct ggttggtacg
    38641 tctggttttc gcacaaagga ttaccgcctg gcttcgcgat tgatcaatga aaaaagaatc
    38701 gatcttgagc ctgtcttgtc ctctctgttt cgccttgatc aggttgggga agcgatcgaa
    38761 caagtagagc agcaaaccat attgaaagca atgattgtgg agtaaatgcc atgaccgata
    38821 gaattccaac tcccattatg ttgatcagcc atgacggatt ggcggcagcg atggtgcgtg
    38881 ccagtgcaat gatcatgggt gaacaggaga acatcggtgt ggtaggtctt gaagagggcg
    38941 atgggctttc aaatttcatt gggaaagtgc aagataacat tgctgattta aaggatgcag
    39001 gacagggaat catttttttg gtcgatatcc cctttggaac gccttggaat gcatcggtcg
    39061 cagtgaaaag agaaattgac catattgttg ctggtgtcag ccttccgttt tttttggagg
    39121 tattgaacta tcgcaatcaa tataatgaga ttgaaaaaat cctgacggat tcattgtctt
    39181 gcaaagacca gtgttctgtt atttgggttc attgaaacgc taaatgaagg agaagaaaat
    39241 ggaatttgga aaacaacgta gaatgcaacg gatcttcaac acagacggtc gtacgctggt
    39301 ggtggcgatg gatcatgctg cgtatatgcc tgatgtggtg ctgggtttgc aagatccagg
    39361 tcgctacatt caagagatcc atgcggctgg agctgatgcg gtcatgacca cactgggaac
    39421 cattcggagg atacaatcca aaatcatcga ttttcctgtc atattaagcc tggaaagcgg
    39481 ctataccaat ataggcgatt tcgtcgaaca aggtttgcgg atgggggtag acatggtgaa
    39541 atgcatggtc tatccttttt cagcggaaga tccttccagt ctgaacaatt ttgcgcggct
    39601 ggcaaatgcc gccgatgcat ggggaatgcc tgtaatggca gaggtgtttc cgggcggaat
    39661 gaatggcggc aaggaatgga atactgttga aaaattgtct tcctgtatca gggttactgc
    39721 tgaagccggc gcggatgtga taaaaacttt ttaccttgat aacaccggcg atttccataa
    39781 cgttattgag aactccctcg tccccctggt ggtattaggg ggtgtaaagt cagatgaccc
    39841 actgcctctg ctggagagaa ttgcactgtg tcttgagaaa ggagcgtctg gagtggcgat
    39901 cggtcgaaac ata
//
LOCUS       DAOSDS010000011        35877 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_608_length_35877_cov_5.348250, whole genome shotgun sequence.
ACCESSION   DAOSDS010000011 DAOSDS010000000
VERSION     DAOSDS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 35877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35877
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_608_length_35877_cov_5.348250"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            369..1547
                     /locus_tag="PLL88_02580"
     CDS             369..1547
                     /locus_tag="PLL88_02580"
                     /inference="COORDINATES: protein motif:HMM:NF018877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOJ00479.1"
                     /translation="MLKNILPFWMTFTPDTENGGFFGQIDANNQIDNSVPRTAVTCAR
                     ILWTFSHAYLTYQKREYLAMAERAFDYLNTAFWDDNYQGLFWSVDQHGSAVLAHKHFY
                     AQAFGIYGFSEYFKATQNMLGLTRAIQLFNIIENHAFDKKFGGYIESKSREFQTIADM
                     RLSDKDMNCRKSMNTLLHIIEAYTNLLLVWDSPELRNQLYRLITDFNQHVFNSSNDHL
                     FLFFDDDWRPLTNHISCGHEIESSWLLCEAAETIGDAKLTKSARDISLRLADSVLKTG
                     ISPDGSVIYEGTLEEITNPAKEWWPQTEAIIGFYHGYQLSGDEKFAAIIPTIWHYLQE
                     HFIDKSNGGWFKRMTAEGFIDMTSPKVGPWECPYHESRMCFEMMKRMRAQEKDNASLK
                     "
     gene            1531..2553
                     /locus_tag="PLL88_02585"
     CDS             1531..2553
                     /locus_tag="PLL88_02585"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOJ00480.1"
                     /translation="MLLLNSQPIPNLPWEDQPAGSADVIWRSSRNPIIPRDLIPSSNS
                     IFNSAVVPFKGKFAGVFRCDNKARVQQLHVGFSEDGFQWQLSNERIIWKSASQKTQDI
                     TKFIYSYDPRVVWIEDRYYVTWCNGYHGPTIGVGYTYDFANFFQLENAFLPYNRNGVL
                     FPRKIHGNFAMLSRPSDTGHTPFGDIFYSESPDMKYWGKHRFVMGATTGWQATKVGAG
                     PIPLETSEGWLLIYHGVLTSCNGFVYSFGAALLDLDQPWKVIQRSEPYLLSPQMIYEQ
                     VGDVPNVVFPCAALVDEPTGRIAIYYGGADTVVCLAFGYVQSIIDFIKSNPMKKPSQI
                     ISDLYS"
     gene            complement(2609..5026)
                     /locus_tag="PLL88_02590"
     CDS             complement(2609..5026)
                     /locus_tag="PLL88_02590"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00481.1"
                     /translation="MQYGYFDVANREYVITNPKTPLKWINYIGSRKFGGFVDHTGGAL
                     ICSDDPALNRITKYIQQMPSSDFKGETLYLRLRQDHGYLIFSPFYVPTLCDLESFECH
                     VGLDYTRIITRGYGVQTEVTIFVPKNEHCEIRDIRIKNISSQPLEIDAIPVLEYTHPD
                     ALGQLTNADWIPQTMQSKAVVGEDGNIILLQYPFMLRDTRINYFTSNQPVSSFETDRK
                     IFLGDNGYGSFKNPTSLYQKSLSNSEAQRGDNMAALLHSLGIVDPGEEKQIITVLGQA
                     PTIADARPLISKYKDPVNVERALNEISEFWDQFLQSFSAKSPDAELDVMVNLYNPRQC
                     HTTFTWSRYLSYYQLGLGARGIGIRDSSQDLLAVLSAIPAEAKQFLKWLLSFQKMDGS
                     AMHQFNPLTNVGSCGDSLEMEDRYHFYSDDHLWLALGIVAYINESGDLDFLNEEIPYY
                     EKDKDEKPVEFGTVMDHVERAFDFTGKHVGSHGLPLLGFADWNDTVNLPKGAESFFSA
                     NLFGLALKEIIPVITFLGKSDLVARYQRIYQEMKERVEQFGWDGSWYLRYFDHLGNPV
                     GSSKNEYSKIYLNGQSWSVLSGFASPERGRTAMDAVFEMLFTKYGIKLSTPSFNGYDP
                     IYGGITTYPPGVKENGGIFVHPNPWAMIAETILGNGDRAYQYYCATNPALRNDLIEVY
                     ECEPYVYSQNVISDEHPQFGSARNSWLTGAAAWHYLAATQYILGIRPELNGLRIDPCI
                     PSRWNGFHVRRKFRGKFISITVHNPDHVCKGVHHMTVGENDLTHNLIPCELIRANTVV
                     EVFLGKE"
     gene            complement(5030..6064)
                     /locus_tag="PLL88_02595"
     CDS             complement(5030..6064)
                     /locus_tag="PLL88_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOJ00482.1"
                     /translation="MEKHELEKGINLGGWLSQYRIYDHSHFKTFIQKKDIRRIAAWGF
                     DHVRLPVDYPLFEDDRKPGEYLQSGFAYVQKCIDWCRRSGLRIILDLHKAPGYAFDDQ
                     QSNRLENDRDLQDRFVELWLALAKEFKSADQDVLAFELLNEINFQSSKKWNGMVEKTL
                     RGMRAMDPERLIVVGGNCFNSVDYLSEIQILPDAQILYAFHFYLPHSVTHQKAYWFEG
                     LDVFDSPVDYPGVAVGLKSFLKDHPQYQDRLGEDVDVHFDRSYLEKRIEPALHFLMAT
                     RRALYCGEFGVIDRAPMGTRENWTRDVVSILKDHYIGYAYWTYKMLDFGLVNEAGEII
                     NQKLIDILVG"
     gene            complement(6073..8529)
                     /locus_tag="PLL88_02600"
     CDS             complement(6073..8529)
                     /locus_tag="PLL88_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOJ00483.1"
                     /translation="MQYGFFDDEHLEYIITKPETPLPWINYLGCEDFFGIVSNTGGGY
                     AFYKDARLRRLTRYRYNNVPADTGGRYVYLRDNDASISAGNSYWSPTWQPVRRPLDDY
                     SCHHGLGYTRIESTFHGIHSTICYFIPLGENLEIWDVRIQNKRDRKADLSLFSAVEFC
                     LWDAWDDATNFQRNLSIGEVEVEGNVIYHRTEYRERRNHFAFFSCSQPIDGFETQRED
                     FLGAYRGWDTPRAVEENKTHNSIASGWYPIGSHHIHAELEPGEEKRIIFLLGYHENPV
                     ENKFVGDSLLINKTSVVSTIQKYSQSKQVDRSFEELGTHWRRLLSIFHATTPNIHVNR
                     MVNVWNAYQVMVTYNISRSASFFESGIGRGIGFRDSSQDLLGFCMLDPLRARQRIVDL
                     AATQLETGGTYHQYQPLSKKGNDDIGGGFNDDPLWLVLAVAEYLKETADWEVLNEMIP
                     FDNKPHSEESLFSHLQKSMQYTLDRLGPHDLPLIGRADWNDCLNLNCFSDTPGQSFQT
                     TTNKDGVTAESVLIAGLYILAAQEMVQMARIRQEEKLAQKYEDAAKKMEQAVWASGWD
                     GKWFRRAYDYYGDAVGSEKNEEGKIFIEPQGICVMAGLGIEGGKAEQALDSVAEHLAT
                     RDGILLQQPAFHRYSLNLGEISSYPPGYKENASVFCHTNPWIMIAEAKIGRGDKALDY
                     YLRINPSVREEMSEVHKCEPYVYAQMISGRDAPVRGEAKNSWLTGTAAWNYVAITRWI
                     LGIRPVYEGLQIDPVIPDTWGNFSVTRKFRGVQFHISVQRKGAGRHCILCVDGIRQKG
                     NVIHMNDIKKPAVDVLVFIE"
     gene            complement(8606..9541)
                     /locus_tag="PLL88_02605"
     CDS             complement(8606..9541)
                     /locus_tag="PLL88_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00484.1"
                     /translation="MLTLNNVSKSYRIGTFGGKELHAVRGVNFTIDKGEIVSLIGESG
                     SGKSTIGKMILHLLSITSGSIVFNDKDIATLRGEELKNYYRKVQGVFQDPYSSYNPIF
                     KADRIFHVIHDEFYPDLMMSAWNEKVHTALEDVGLNPQHVLNKFPHQLSGGQLQRFLI
                     ARALLLELELLIADEIISMLDASVRIDVLNLLATLQAHGLSVLFITHDLSLANYISKN
                     AIILYHGFITEKGNTEKIFNDPLHPYTQMLMASVPRLDGKWKAPSDQDSSVVSPQGNG
                     GCVYQERCPYAFEKCKETPVYVEVEKDHFVACWKV"
     gene            complement(9543..10493)
                     /locus_tag="PLL88_02610"
     CDS             complement(9543..10493)
                     /locus_tag="PLL88_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00485.1"
                     /translation="MSLIVEDLTVYYQTLKGDVHALEDVTFNVKSGEIMGLAGESGCG
                     KTTLCQSLIFLNPPMKYIKGHVTLNGTEMPIWDNEKMNDFRFHDISIIPQYAMNAMNP
                     IRRIGMLTRELLAAKGVDYKEKLPEIKHRLDLVNLPHDVLKMYPIELSGGMKQRMVMV
                     LSTLLNPTVLIADEITSALDVASQKAVVEMLIEFLREGCCKSIIFITHDISILYQIAN
                     TILVMYAGLLAEKASTETIIHAPRHPYTKQLIASLPKIGAKYSEERLKGIPGQPPLLL
                     NPPLGCRFRERCPVAFEKCIETPPFVEIKPDHHVACWREM"
     gene            complement(10497..11357)
                     /locus_tag="PLL88_02615"
     CDS             complement(10497..11357)
                     /locus_tag="PLL88_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00486.1"
                     /translation="MKKQKHKNETLYFAFRNWKLKIGLFIIIFFLLISIFGPSLSPYK
                     PFDYVEPQGALAPSAKHWLGTTFFGQDVYAQFVSGLRATFQVGIIGGGIGTLLGLIIG
                     FIAGYKGGVVDEILNALTNIVLVIPTMAVLLIIAAYLQVRSVFVECLLIGFTSWPWTA
                     RAVRAQTFSLRTRDFVDLARLSGMKSKKIIFSEIAPNMMSYLLLVFILQFGGAILTAA
                     TLDFIGLGPTNAISLGLMMQYSVLWGALQFGMWWWFVPPGLAITIIVGALYSMNVGLD
                     EVFNPKLREM"
     gene            complement(11354..12352)
                     /locus_tag="PLL88_02620"
     CDS             complement(11354..12352)
                     /locus_tag="PLL88_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00487.1"
                     /translation="MKKYFLRKILIYLITFFLAVTIDWMIPRFMPGDPVRNMLTRVSV
                     SSNATAIQMMYDYYSHLFGLDLPLGQQYLNFWKLLFQGNLGISTYLFPTPVTKVLARA
                     VPYDVFLLVPSIALSWYAGNKFGAVAARSKKLDNTLSPIGYILNATPYMWLAILLAWF
                     FGIVLGIFPIAGAYSFSLQPSFSLKFIASVFHHWFLPFASVFLVEFGGQAIGMRNLII
                     YELESDYSHYLGALGAPKKLIRKYAFGNAILPQITGLALKLGVIVAGALVTEVVFSYP
                     GIGYLLLAAIQNQDYFLIQGCFLFIIVGVLIANFAIDIIYILVDPRTRMGMTGETA"
     gene            complement(12454..14214)
                     /locus_tag="PLL88_02625"
     CDS             complement(12454..14214)
                     /locus_tag="PLL88_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ00488.1"
                     /translation="MRKNILFLLSIFIVAGVVLAACQPAAEAATTEPAAEGPAVEEAA
                     PTEFPRNETIYVSGAAWGPASTWNPFQPGSLANTTGTIGFVYEFLYAFDPQTGELTPW
                     LAESGTWVDDTTFVVTLRPGLTWSDGEPLTAEDVAFTFNLGTQYAALWFSPMWAYTES
                     VTALSDAEVEFKLSAEPLYQEFENNLYNVPIVPKHIWESKSEEDITVGANENPIGSGA
                     YKYLSHAEDRNIWIRNEAWWGIDVFGAPAPKYIVDIRTSSNNVALGMVLKGELDLSTN
                     FLPGVAEMADKGYVKTYFADAPYMLSANTAVLFLNTTLKPLDDAAFRRALAFAIDVPS
                     IVNVAYAQLVQAASPSGLLPVLDKYVDQDVMAELGYSFDTAQAKQMLADAGYVDIDGD
                     GFVETPDGEKIELEVTCPSGWTDWMAAIDIIASSAQAAGINLKSAYPDYGAWNTALQG
                     GTFNMTLNNWASMSNTPWTLYNLLFRHPIQEQMQSGNFGRYENQEVFDLVDNLAKIPM
                     SDEEGMQAACSAIQRAMLTDMPMIPLWYNGLWAQYSNSTWTNWPDESSSSPTLPTTWS
                     GYWQLGGLQTLINLEPVTAE"
     gene            complement(14352..15371)
                     /locus_tag="PLL88_02630"
     CDS             complement(14352..15371)
                     /locus_tag="PLL88_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ00489.1"
                     /translation="MNNLTLEDIAKKSGVSRSTVSRVINNHPNVSKKVREHVKEVIRS
                     TGYQPHPAARALVSNRTWTCGLILPRTINSFFTDPYYPCLIQGIAQACSKQNYTFSLF
                     LMGDKEDEDQMFSRVSRRGFMDGILFQSDQRGDTLIDLLADSGIPLVVLGRPFQGEQI
                     SYVDIDNTESVEWAVQYLLDNGHKRVATITGPQNTTVGIDRKYGYKQALVKNNIPVDE
                     EMIVEGIFTEESGYLGMKKLLPKMPDAVFAASDQMAIGAIRAIREAGLNVPQDISIIG
                     YDDFPLVSPVYPALTTLRQPIGKFGFEALKMLISLVEEGLKPSRQVILKSELIIRDST
                     RNRIL"
     gene            complement(15611..16135)
                     /locus_tag="PLL88_02635"
     CDS             complement(15611..16135)
                     /locus_tag="PLL88_02635"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ00490.1"
                     /translation="MPDTHKQFTAVPPMQIDIKKEYSVLVKMAKGGEFTITLYPAKAP
                     ITVNSFVFLVRQGFYDGVTFHRVLDGFVAQGGDPTGTGAGGPGYEFMNEDSDLKYDQA
                     GVVGMANAGRDTNGSQFFITYAPLPRLNGGYTIFGQVSEGMDVVNGITRRDPGKNPNF
                     SGDVIEKMTLIEAE"
     gene            complement(16265..16714)
                     /gene="rpiB"
                     /locus_tag="PLL88_02640"
     CDS             complement(16265..16714)
                     /gene="rpiB"
                     /locus_tag="PLL88_02640"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOJ00491.1"
                     /translation="MQKHIVIGSDHIGYPLKEHLKTILEKQGWEVEDCGTYHRARTDY
                     PLYAEKVAQKINSGEYERGILICGSGVGMSITANKIHGIRAVVCSEPYSAVLSRKHNN
                     TNVLCMGSRVVGEELAEMILWDWLAADYEDGRHQHRLDLIKELEDKY"
     gene            complement(16723..17673)
                     /locus_tag="PLL88_02645"
     CDS             complement(16723..17673)
                     /locus_tag="PLL88_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00492.1"
                     /translation="MVKRILSCTRSDFEKMTPMDLKLSIRLAEGRTILAQSFNAMGNA
                     LLNGVTNPELEAAFGADILLLNGYSFDEKSPLYGVKHYNYREGLQTYHVGDIKKLVNR
                     PVGVYLECVSAETDHLYVSGFGLGRTATEENFCKAVEEGADFIILGANPSMNAKIDDI
                     IAATKLAGKTVKGKTLIFSGKWEDGVYEKVLGDPMAKKPAREFIDALMDAGADVIDFP
                     APGSRQGITVDMIRELVEYVHHKDIDTMTMSFIDGSVEGADVDTIRILALQSRETGAD
                     IHAIGDAGLSGCSLPENIYQLSLSVKGRRLTWERMAGNHR"
     gene            complement(17793..19064)
                     /locus_tag="PLL88_02650"
     CDS             complement(17793..19064)
                     /locus_tag="PLL88_02650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOJ00493.1"
                     /translation="MQTKKKSFNEKIFEIIDTRLGPVFAKIGENKIVRTISGGMMLTV
                     GVLTIGSIFLMLFGLATNYSFFSFLLPIAGPLLIGYQLTMGLTGMFVAVAISGAYAKQ
                     YGMELLSSALTGLAAFLLLTSTISDGTIAITNLGAQGIVAAIISSLVAMSILRLFKEK
                     NITIKMPDGVPPEIAATFSSLIPSFLVLVLMWAVKSVFNFDLVTAVQKLLAPIFSAVD
                     SIGAFTLRFLLGQAVWSVGLHADAILTGITGPLTSAWIIENADAAVAGVAATKLPYIW
                     VQATERMCGWTASIWGLMYWLFRSKSKKLRTIATAGLPSAIFTIIEPILFGLPVVFNP
                     YLLVPFILSGTLAAFFTYGVMALGLVSRFYVSLPWITPPPVLALLGTGGDWKMVVVVI
                     INTLIGILVYAPFFKAYEKSELAKESDIKAK"
     gene            complement(19132..19446)
                     /locus_tag="PLL88_02655"
     CDS             complement(19132..19446)
                     /locus_tag="PLL88_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HOJ00494.1"
                     /translation="MHESLEIIAMEMISFAGDAKADAYHALHAAKRGDFEEAKRLLES
                     AEEKLTLSHRTHLKLLGIENQFKSINEQLLVTHAMDTMMTSSSEINLINELVDILKEK
                     VR"
     gene            complement(19446..19748)
                     /locus_tag="PLL88_02660"
     CDS             complement(19446..19748)
                     /locus_tag="PLL88_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00495.1"
                     /translation="MKILIICAVGMSSSLVVDRMRKLAPEGTVIDYGIGMDTIKEIGD
                     YDVLLIGPQLRYKKDQIVAAAEKEGIPYGFIDMVHYGRAMAQEIYDQALELHKGKK"
     gene            19869..20624
                     /locus_tag="PLL88_02665"
     CDS             19869..20624
                     /locus_tag="PLL88_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ00496.1"
                     /translation="MLPIQRQLKIKEFLVEKGFASVEELSKQFEVSEMTIRRDLDELH
                     EQGVIQRIYGGAMDNDQAFFEMSVNAKAGKFATEKVKIGRAAADLVQNGDTVFMDSGT
                     TTLEVAKNLKNKKITIITTALNVAVELSASPDIDILVAGGILRKGPLNTTGPQTDAFI
                     RELRVDKVFIGVEGVDLESGISVPDPINAHNKHTMIEIARQVIVVADHSKLGRTTTSN
                     IAPLDKVHVVITDNGADPAIIEELRKKTQVILV"
     gene            20690..21355
                     /gene="rpe"
                     /locus_tag="PLL88_02670"
     CDS             20690..21355
                     /gene="rpe"
                     /locus_tag="PLL88_02670"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOJ00497.1"
                     /translation="MKNNIALGASLMCANYLNLEHDIRLLEENGIDYLHFDVMDGHFV
                     PNFGLNLDLLQKVRKITTLPINVHLMIEDASHYIDTFIDAGCNSLSFHHEANYHVQGL
                     LQKIRDRKVKAGIALNPATSLSSLEYILNDLDFVLIMTVNPGFAGQPLIPATLEKIAD
                     LKRIIRAKKLAIDIQVDGNVSFENIPKMVQAGATMLVGGTSSLFLKDLSIPEAVQIVN
                     NLL"
     gene            complement(21424..21588)
                     /locus_tag="PLL88_02675"
     CDS             complement(21424..21588)
                     /locus_tag="PLL88_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00498.1"
                     /translation="MCIKEIHRALKPSEKRYIGGVAVRSPRFSDKKDLAFYSDGRSPS
                     KLVQAGYGYP"
     gene            21765..23381
                     /locus_tag="PLL88_02680"
     CDS             21765..23381
                     /locus_tag="PLL88_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00499.1"
                     /translation="MDTIKRKYASFILSNFPRVLSQIDRDPDSPTFGCCDRNYWHYKI
                     RDFSSAILQQSGLVLAKLYHIDFPENPLFQNQNAAHIALATVNFWKEIQLGDGSFNEY
                     YPHEHGFPPTAFSFYAACETYRELGLDESAYLEAFAKTATYLSKHVESQASNQEMASI
                     TALYSYYLISKEQAVKQAVERKLERILSIQSAEGWFPEYGGADFGYQSVCLDMLAEYF
                     HLSHDERVLEPMQRVVEFLKWFVHPDGSMGGEYGSRNTIYCLPFGIELMTARGDAAAV
                     AMRSAIFGGCDQPDYFQNSIDDRYFSHYVLHSFVRAYELPGENAGTKIPLPYQEGGYR
                     YFPDSGLLSKTTKNYFAISSLKKGCVKVFQADHFSFADFGYRIVLGKSTLGATNWLDE
                     KNEYSYHENENRAVIRGRFTKVRIQTPSPLNHAGLRLASFLFGNRLIGFLKKKMIFID
                     SHLEVRFERTIEFQEDKVLIKDKIESPEKVTIKSAPPFSLRHVASSKFFSSSDLAERG
                     EVLLKEIKQAMIEIEFDGKTGQTNIFSTGE"
     gene            23384..24031
                     /locus_tag="PLL88_02685"
     CDS             23384..24031
                     /locus_tag="PLL88_02685"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ00500.1"
                     /translation="MDLDEAIKGRRSIRRYSERDIDEETLEQIIQAGMWAPTACNDQD
                     YRFIIINDKNKFDELLAYGAATFIKDVHTAILVLYKNTSANQEYKDYFQSGSAVIQTM
                     LLKAHSLGLGSCWVCNLPSQKNMRHIFNIPANYSTIALVSMGYPLSPPKPVQRKHKIN
                     DVVCLNAFGFNNEENDPTIERKKYLKRFLRRVYYRLPKSAFIRRWAEKLEKKFVN"
     gene            complement(24028..24624)
                     /locus_tag="PLL88_02690"
     CDS             complement(24028..24624)
                     /locus_tag="PLL88_02690"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ00501.1"
                     /translation="MSDQISEGLLSPFLRSMRIRQALPFIEGRILDIGCGSGELASHF
                     SPEDYWGYDPDEQSIAHAREKFPTHSFSHELPDRKQKFNSIVLLAVIEHISDPVSYMT
                     LLQSYLASGNRSSIILTTPAVSTKGIHSIGSRLGLFSIEADHEHKMLFNQKALTDLSR
                     ECKMNLIHYHLFSFGLNQLAVYKRKDGIETSPANQGDR"
     gene            complement(24746..25243)
                     /locus_tag="PLL88_02695"
     CDS             complement(24746..25243)
                     /locus_tag="PLL88_02695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOJ00502.1"
                     /translation="MKPALLVIDIQKQFYRTSDETARSLQDAVEYANEAIKVFRKKQL
                     PVIAIQHLNKENGLVPENPDFDLPDDLKINSADLHIQKTHGNAFVKTPLLDELKKMNV
                     DTVVLCGFCAEYCVFATYHGAKDVDLFPIILRGGLASDTPKNIAFVEEICESMSLGVL
                     EKILE"
     gene            complement(25388..26101)
                     /locus_tag="PLL88_02700"
     CDS             complement(25388..26101)
                     /locus_tag="PLL88_02700"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOJ00503.1"
                     /translation="MEAIVQDVRQTLQNSSDEKTKASSQRFFKEPVKCYGVNVALVGK
                     IGDQYWKQICHLSKTAIFALCEQLLSTGYCEDAYIVSGWLPKLNNEFQASDIHTFEGW
                     IEKYIDNWAKCDGFCNHTVGDLVMKFPQNIAELKTWARTDQRWLKRAAAVSLIIPARK
                     GMYLQDIFEIALIVLTDGDDMVQKGYGWMLKEASRLHQDDVFQFVMEHKKNMPRTALR
                     YAIELMLPNMKKEAMAKDW"
     gene            complement(26111..26569)
                     /locus_tag="PLL88_02705"
     CDS             complement(26111..26569)
                     /locus_tag="PLL88_02705"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOJ00504.1"
                     /translation="MKYDCRIIEAKDQPVLVVRTITAVENLPAFFGEAYGSIMQYLEE
                     LGEQPVGMPFAAYYNLDMKALDVEAGFPVARSLPAKDKIHSGMIAGGKFGITLHVGSY
                     DTVEPAYEALTAYVKQQGFEPTGVAYEYYLNDPGEDPSIKPETEIRFPLK"
     gene            26709..27707
                     /locus_tag="PLL88_02710"
     CDS             26709..27707
                     /locus_tag="PLL88_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOJ00505.1"
                     /translation="METIWLTEVGITQFFQNAGAWLLLPMQLITFLGNEQFYMLVMPA
                     LYWCVDSVLGLRVGVMLLTSGILNGTAKLIFKSPRPYWFDEQIAAHSSESSFGMPSGH
                     AMNSISVWGLLAASIRKKSVTVILAVIIFLIGISRIFLGVHFTSDVLVGLLLGALLLI
                     VFLHVEKKVAAWVSRLTLGQFAAIMFLISLAIIAITALIIRANVDWRIPSAWITNAME
                     AAPEGEIDPFSLDGTITNAAVLFGLAIGAVWMNRRGGFNAGGVWWMRLLRFAIGVSGV
                     LLFWAGLGMVFPDSADLFGFALRYIRYALTGLWISAGAPFLFVRFNLAGKKETKEG"
     gene            complement(27755..28060)
                     /locus_tag="PLL88_02715"
     CDS             complement(27755..28060)
                     /locus_tag="PLL88_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00506.1"
                     /translation="MPILDDLSKGLKKGIDEAGKGLKEVSEKASDAVKTFEINQEIGK
                     LEDEIKGLKVEIGEKALELMAKGVALDPSIEELAKKILGIDAQIEGKRARIQEIKND"
     gene            complement(28236..29198)
                     /locus_tag="PLL88_02720"
     CDS             complement(28236..29198)
                     /locus_tag="PLL88_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOJ00507.1"
                     /translation="MSKPTSNQAYFIALASAIFLSTTAIFIRYLTVQYGLPALVLAFW
                     RDFFVVLTLFPILLFKQKKLLLLNRKQAPYLLAYGLVLAVFNAFWTISVSLNGASVAT
                     VLSYSSAAFTALLGHLLLKEELGWVKIVAIVCSLAGCVLVSGAYAGDAWQTNLVGIVS
                     GVLAGLSYAAYSLMGRHAAQHGMDPWTTIFYTFAFAGLCLLAINLIPASMIPGIAQEP
                     ADLLWLGTDWKGWLPLFLLAAGPTVMGFGLYNISLQALPASVANLIATSEPVFTSLFA
                     YLVFGELLGKIELAGGMLILAGVVLLRLVPNAGKSKTLESSAQE"
     gene            29377..30315
                     /gene="yhfZ"
                     /locus_tag="PLL88_02725"
     CDS             29377..30315
                     /gene="yhfZ"
                     /locus_tag="PLL88_02725"
                     /inference="COORDINATES: protein motif:HMM:NF041241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HOJ00508.1"
                     /translation="MENDLLRPLISQQGKVVIALAKIFLNHKPGEKIERYQDYAKRFQ
                     VGTGTVQAALKYLQDVRAISLSTKGHLGTFIDKIDYPQLCALAGQDIIVGGMPLPYSR
                     RYEGLASGLNKAFAQANLGINLLFCRGSMNRIKTLMQGRYDFIVLSKYACDSAKAMGW
                     KIRDIFDLGIETYVGQHAIFLRDQSKQSIENGMKVGIDPDSIDQINLCKEVTKNLKVE
                     FVEISYMNLSSALRDKQIDATIWNTDDFATSDSAFKIISLEKNQITQNLDNTVAVIAV
                     KEDHQSLIHTFQSTIDVELILDIQRKVMSKELLPMY"
     gene            30330..30716
                     /locus_tag="PLL88_02730"
     CDS             30330..30716
                     /locus_tag="PLL88_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HOJ00509.1"
                     /translation="MPFTDRFKFLLKSKQASPASIRITEELVDLIEKEYHTKLDESNG
                     ASLVTHLAITIKKLTAHEKLSKIPDVCLTEALSFKNEMKFANRLTTYLKETHQIDFNR
                     SETAFLTIHLRNVSQHLERKEEKKRN"
     gene            30742..31113
                     /locus_tag="PLL88_02735"
     CDS             30742..31113
                     /locus_tag="PLL88_02735"
                     /inference="COORDINATES: protein motif:HMM:NF022394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 family protein"
                     /protein_id="HOJ00510.1"
                     /translation="MIKVNIGGMNAQEIKDLIDKFAEGKIEAKITSDMSGAQEVAQGE
                     ADYYFGACATGGGGAISMAIAILGYSQCFTASMPGKPPRKEEIEKAVRDGKKAFGFTC
                     DHVQLAVPLIVQAISENSSAK"
     gene            31129..32433
                     /locus_tag="PLL88_02740"
     CDS             31129..32433
                     /locus_tag="PLL88_02740"
                     /inference="COORDINATES: protein motif:HMM:NF022254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HOJ00511.1"
                     /translation="MSVIKIVVVAIAGALGSALANRGIAVYHDGLRPIMPELIEGRMK
                     RAEMAAISFGLSFGLVIGFGIPFSLTSSIILVHSLWLGTDIIGTWFPGSFEKGWMKDK
                     KSLWGFVGSLVAGGLYGGLLVVGLQAIVTLFEKLPINFFGSLGSLGDPILYAFAIFPA
                     IAVAMQFGILKGIITFILTILARQIATAAGLSSADGIALAVGILLLMGFAIADKPKGE
                     KKSQPSMQALFTERANRIKSNLPYIAVMGAVYGIACNLAIMMEGPQSITALAAGDRAA
                     ATSISVARAISFIPLKAMTSLTSGVFAMDGFGFVATAGLLSPSWIVAAVAGAAVMSLE
                     AYSLVFIGKLLDKFPGVRNAADNIRTSMTKLLEVATLVGSMIAAHTMAPGLGFLVVAG
                     AYVLNEITGTRIVRMAIGPIAAIFTGILINILAVVGLYTPPV"
     gene            32509..33552
                     /locus_tag="PLL88_02745"
     CDS             32509..33552
                     /locus_tag="PLL88_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ00512.1"
                     /translation="MQFRLVECISHHFDGESILQAGDYGVNQKTGRPLHTARAEKVLA
                     EFFHAEDACLVRGAGSGAIRSLFMAMAKPNQEILLHKAPIYPTTAVTIRAMGLKPVFI
                     DLNHPQEINKADVARAQLGIIQHARQQMEDRYSLATSITSLKKINPSICLITDENYLV
                     CKVERIGIELGADASTFSTFKLFGPEGVGCILSSRKVIDAIRKDLYSGGSQVQGVEAL
                     DTLRSMVFAPVAFAIQKEVVDETVKRIKNEKIAGVRDAYIANAQSRVILVEFEKSIAK
                     KVLQNAVKLGAAPHPVGSESRYEIAPMFYRVSGTFLVDDSSLADTMIRINPMRSGSDL
                     IMDILTKSIEQAG"
     gene            33556..34731
                     /locus_tag="PLL88_02750"
     CDS             33556..34731
                     /locus_tag="PLL88_02750"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOJ00513.1"
                     /translation="MFLDQIRKYNPDLLSVALQFHRAGKIPPSCFVIDLDTVIENTRA
                     LVQTAKKNDLTLFFITKQVGFNPVFAKEVWKNGIAKAVAVDWNEAVSLIKNKIPIGHI
                     GHLVQIPDCFMETMLRANPEVITLFSIEKARKLSQVAQKENLPANVLLRVVDNGDFVY
                     SGQTGGIPLSALERSLDVLNTFPNIRIAGVTAFPCTAFDPLQNKFVPTKNLHTLGKAA
                     EIIARQYSHARLQINAPGNSCCDTFPLIQRYGATHAEPGNALTGTTPLHAVSSQPEKP
                     ALIHVTEISHEYKNVYYCFGGGLYRRSTVTKALVGNEPTALKEARVISPEKTSIDYYV
                     GLKMSTKERAEIGDSVLWSSRAQLFVTRSSVAIVKGIRHGKPELAGIYNPLGWKTDF"
     gene            34673..>35877
                     /locus_tag="PLL88_02755"
     CDS             34673..>35877
                     /locus_tag="PLL88_02755"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF009049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOJ00514.1"
                     /translation="MGSRNWLAFTIHSVGKPTFKCETMKRAVLLVLDGLGIGAMPDAA
                     KTRPQEQNANTLGCILKHRPHLHIPTLTRMGLMHCLNRVQDGAAELLASWGWCELAYR
                     GADSYLGHQEIMGVIPLDPQRTLLRDEIDGFEKILTARGFQVTRPLAGKPLLCINDAI
                     IVGDNLEADAGQNINLTVCTDRIEFGKAVEIGKIIRSAVRVARVIVFGGPGLTLQRIL
                     EHVEQRENGQVGVNSPAIGVYNEHLLVKHMGFGVDPQFQAASIFSQAKKDVFLDGKMA
                     DLIECEQAVKSPIVETQALLDCVQTQFRSLEDGLIAATVQETDLAAHGGEIDRFADIL
                     ELVDRFLCGFLPQMKKEDLLVITADHGNDPGLRTGLHTREATPLLVFSPQFRPTPLGK
                     RKCLADSGAS"
BASE COUNT         9750 a         8848 c         8005 g         9274 t
ORIGIN      
        1 agcgtaaata gtgataacgc agtggaatta atctctcagc catcgatcga tggtctgttt
       61 attggaagag ctgcctggca tgtagatggt tttctttcta tcataagaaa tgtagaatcc
      121 tttctgaggc aataccattg acctatttgt tcaatagatt ccattgcctg gcaaaaccat
      181 ctctttgacg tttccaaggc agttgcgtta tttctctttt aatatttcct cccttgatga
      241 atagttcttt tatgaaatgg atgtataatt aactttattg ggagcgttcc caatattttc
      301 tgccttttca aagaaaaact atagccagtg agggaaatcc gttggaacca gaactatttg
      361 agaacgaatt gctgaagaat atcctaccct tttggatgac ttttacacct gatacagaaa
      421 acggcggatt tttcgggcaa atcgatgcaa acaatcaaat cgacaacagt gttccacgca
      481 cagcagtcac ctgcgcacga attctctgga ctttttctca tgcatatctc acctaccaaa
      541 aacgggaata tctcgctatg gctgaaagag ccttcgatta tctgaacact gccttttggg
      601 atgacaacta tcaggggttg ttttggtcgg ttgatcagca tggcagcgcg gtcttggctc
      661 acaaacattt ttatgcacag gcttttggaa tctacgggtt cagcgaatac ttcaaggcta
      721 cacaaaacat gctggggctt acacgagcca tccaactttt caacattatt gaaaaccacg
      781 cattcgataa aaaattcggc ggatacatcg aatcgaagag tcgagaattt cagaccatcg
      841 ccgatatgcg cctcagcgac aaggacatga attgcagaaa gtccatgaac acactgctgc
      901 acatcattga ggcatataca aatctgctcc tggtgtggga tagccctgag ctcaggaatc
      961 agctttaccg tttgatcact gattttaatc aacacgtttt caatagtagc aacgatcact
     1021 tgtttctgtt tttcgatgat gactggagac ctctcaccaa tcacatttca tgcgggcatg
     1081 aaattgaaag cagttggctt ttatgcgaag cagccgaaac gatcggcgac gcaaaactca
     1141 caaaaagcgc cagggatatc tcgcttcgac tggctgattc cgttttaaaa accggaatca
     1201 gccctgacgg aagcgtcatt tacgagggca cgcttgaaga aatcaccaat cccgccaaag
     1261 aatggtggcc acaaaccgaa gcgatcatcg gtttttatca tggttatcag ctttccggcg
     1321 atgaaaaatt tgcagcgatc attccaacca tctggcatta tttacaagag cacttcattg
     1381 ataagtccaa cggtgggtgg ttcaaacgaa tgactgcaga gggattcatt gatatgacca
     1441 gccccaaagt tggaccatgg gaatgcccct accacgaaag ccgcatgtgt tttgaaatga
     1501 tgaaaagaat gcgggcacag gagaaagata atgcttctct taaatagcca acctattcca
     1561 aatctgccat gggaagacca accagcagga agtgcggatg ttatctggag gtcatcacga
     1621 aaccccatca taccgcgcga tctcattcca tcatcgaaca gtatttttaa cagcgcggtc
     1681 gttccgttta aaggaaaatt cgcaggggtt tttcgatgcg acaataaagc gcgcgttcaa
     1741 caactgcatg tggggtttag cgaagatgga tttcaatggc aactttctaa cgagaggatc
     1801 atctggaaat cagccagcca gaaaacgcaa gatatcacca aattcattta ttcctatgac
     1861 ccgcgagtgg tttggattga ggatcgttat tatgtcacct ggtgcaacgg gtaccatgga
     1921 cccaccatag gggttggata tacctacgat tttgcaaact ttttccaact ggagaacgca
     1981 ttcctcccct acaaccgcaa tggagtgctg ttcccacgca agatccatgg caactttgcc
     2041 atgctcagcc gccccagcga tacggggcat acgccctttg gagatatttt ctacagtgaa
     2101 agcccggata tgaaatattg gggaaaacac cgtttcgtca tgggggctac taccggctgg
     2161 caggcaacca aagtcggcgc gggacccatc ccactcgaaa ccagtgaagg ttggttgctg
     2221 atctatcatg gtgtattgac ctcatgcaat ggtttcgtct acagctttgg agctgcgttg
     2281 cttgacctcg accaaccctg gaaagtgatc caacgtagcg aaccgtacct tttatcaccc
     2341 caaatgatct acgaacaggt aggtgatgtt cccaacgtgg ttttcccttg cgcggcgctg
     2401 gtcgacgaac caaccgggcg aatcgcgatc tattacggag gagctgatac ggttgtttgc
     2461 ctggcattcg ggtacgttca aagcatcatc gatttcatca agtccaaccc tatgaaaaaa
     2521 ccatcgcaga tcattagcga tctctattca taaaaaccac gcaaagtgat cccccatggc
     2581 agttgagaaa gaaaaccagg cagcgcggtc attctttgcc caagaacacc tcgacaacgg
     2641 tgttcgcgcg aattaattca caagggatga ggttgtgcgt caaatcgttc tcgcctaccg
     2701 tcatgtgatg tacccctttg cacacatgat cgggattatg cacagttatt gaaatgaatt
     2761 tccccctgaa ttttcgcctt acgtgaaagc cattccaccg ggaaggaatg caaggatcga
     2821 tgcgcaatcc attcaactct gggcgaattc caagaatata ttgcgtggca gccaggtaat
     2881 gccaggcagc cgcgccagtc agccaggaat tgcgcgccga accaaattga ggatgttcat
     2941 cgctgatgac attttgtgaa tagacgtagg gttcacactc gtagacttct atgagatcgt
     3001 tgcgcaaagc cgggttggtc gcacaataat actggtaagc ccgatcgcca ttccctaaga
     3061 tggtttcagc gatcatcgcc cacgggttgg gatgcacaaa aatgccgccg ttctctttaa
     3121 cgcccggggg ataagtggta attccaccat agatcgggtc atagccattg aaagaaggcg
     3181 tagagagctt gatgccgtac ttcgtgaaaa gcatttcaaa aacagcatcc atggcagttc
     3241 ttcctctctc gggtgatgca aagcccgaaa gtaccgacca gctttgtccg ttcagataga
     3301 tcttgctata ttcatttttc gatgaaccaa ccggatttcc cagatgatca aagtagcgca
     3361 aataccagct tccatcccat ccgaattgct cgacccgctc cttcatttcc tgatatatcc
     3421 gttgatagcg agcaaccaaa tcggatttgc caagaaaggt gatgacagga attatttctt
     3481 tcagtgccag accaaacagg tttgcactga agaatgattc tgcgcctttg gggagattaa
     3541 cggtatcgtt ccaatctgca aaccccaata ggggcaagcc atggctgcca acatgcttgc
     3601 cggtaaaatc gaaagcccgc tcaacatggt ccataaccgt gccaaattca acgggtttct
     3661 catctttgtc cttttcataa tagggaattt cctcattcag aaaatctaag tccccgcttt
     3721 cattgatata ggcaacgatc cctaaagcca accataaatg atcatcacta taaaaatgat
     3781 aacggtcctc catctccaga gaatcgccgc agctaccaac attggtcagg ggattgaatt
     3841 gatgcatggc agatccatcc attttctgaa aagagagcaa ccacttcagg aattgctttg
     3901 cttcagcagg gatggcagac agaactgcca gcagatcttg tgaggaatct ctgatcccaa
     3961 tgcctctggc tccgagacct aattgataat aagagagata gcgcgaccag gtaaacgtag
     4021 tatggcactg gcgagggtta tacaaattga ccatcacatc cagctctgca tcaggacttt
     4081 ttgcagaaaa cgactgaaga aattgatccc agaattccga aatttcgttc agagcgcgct
     4141 caacattaac agggtcttta tatttcgaga tcaaggggcg tgcatcggcg atcgtcggcg
     4201 cctgccccaa taccgtgatg atctgctttt cttcacccgg gtcgacgatc cctagcgagt
     4261 gcagcagagc tgccatgttg tcaccgcgct gtgcttcgga gttagataga gatttttgat
     4321 acagggatgt tggatttttg aagctcccgt atccattatc gcccagaaat atttttcgat
     4381 ccgtttcaaa agatgacacc ggctggttag aggtaaagta attaattcgg gtatcgcgca
     4441 acatgaaggg atattgcagc agaataatat tcccgtcctc cccgaccaca gccttgcttt
     4501 gcatggtttg cggaatccag tcggcattgg tcaattgccc cagagcatca gggtgtgtat
     4561 attccaacac cggaattgca tcgatttcca acggctggct tgaaatattt ttgattctga
     4621 tatcccgtat ttcacaatgc tcatttttcg gaacaaaaat agtgacttcc gtttgaaccc
     4681 catagcctcg cgtgatgatc ctggtgtaat ctaaacccac atgacattca aagctctcca
     4741 gatcacaaag cgtgggaaca tagaacggtg aaaagatcag gtatccatgg tcctgacgaa
     4801 gtcgcagata aagcgtttcg cctttgaagt cagatgaggg catttgttga atatacttgg
     4861 taatccgatt caaggcaggg tcgtcgctgc agatcaacgc gccacctgta tgatcaacaa
     4921 aacctccaaa ttttcgcgag ccaatataat tgatccattt cagcggggtt tttggatttg
     4981 tgatcacata ttctctgttg gcaacatcaa aatacccgta ttgcatgctt caccccacca
     5041 aaatatcaat taatttctga ttgatgattt cgcctgcctc atttaccaaa ccaaaatcca
     5101 gcatcttata ggtccaatag gcataaccga tataatggtc tttaaggatc gagacgacat
     5161 ctctcgtcca attttcccgg gttcccatgg gagcacggtc gatcacccca aattccccac
     5221 aatacaatgc tcggcgagta gccattaaaa aatggagtgc aggttctata cgcttctcca
     5281 gatatgagcg gtcaaaatga acatccacat cttcccctaa cctgtcttga tattgaggat
     5341 gatctttcag aaagctcttc aacccgactg ccacgcccgg gtaatctact gggctgtcaa
     5401 aaacatccag accttcaaac caatatgcct tttgatgagt gacagaatgg ggcagataga
     5461 aatggaacgc atagagaatc tgcgcatccg gaagaatctg tatctctgat agatagtcga
     5521 ccgagttgaa gcaattaccg ccaaccacta ttaacctctc ggggtccatt gctcgcatgc
     5581 cacgtaaggt cttttctacc atcccattcc actttttcga cgattgaaaa ttgatttcat
     5641 tcaacaattc aaaagccagc acatcttgat cagcagattt gaattctttt gcgagagcca
     5701 gccacaattc tacaaaccga tcttgcagat cgcgatcatt ctcaagccgg tttgattgtt
     5761 gatcatcgaa ggcataaccc ggagctttat ggaggtcgag aattatccgc agtcctgaac
     5821 gtctgcacca atcgatacat ttctgcacgt aggcgaagcc actttgaaga tactcacccg
     5881 gcttcctatc atcttcgaag agcgggtaat caaccggtaa acgcacatga tcgaaacccc
     5941 atgcggctat acgtcggata tccttttttt ggatgaaggt tttaaagtga ctgtgatcat
     6001 aaatcctgta ttgtgacaac caaccgccca gattgattcc cttctccaac tcatgttttt
     6061 ccatcgccaa acctattcga taaaaaccag cacatccact gcaggttttt tgatatcgtt
     6121 catgtgaatg acattccctt tctggcgaat tccatcgacg catagtatgc agtgccttcc
     6181 cgcgcctttc cgttgtacgg agatatggaa ttgcacgcct cgaaacttac gcgttacaga
     6241 aaaattaccc caggtatccg gaatgaccgg atcgatctgc agaccttcat ataccgggcg
     6301 gattcccaaa atccaacggg taatggctac gtaattccag gcagccgtgc cggttagcca
     6361 tgaatttttc gcctcacctc tgacgggtgc gtcgcgtccc gagatcattt gagcatagac
     6421 atacggttca catttatgaa cctcgctcat ctcctctcgc acggaagggt taatgcgcag
     6481 gtagtaatcc aatgctttat cccccctccc aatcttcgcc tcagcgatca tgatccacgg
     6541 attggtatgg caaaaaacgc tcgcattttc tttatatccg ggaggataag atgatatttc
     6601 acccaagttc aaggaatatc tatggaatgc gggctgttgg agaagaatgc catccctggt
     6661 tgccagatgt tccgctacgg aatccaaagc ttgctccgcc ttaccaccct cgatgcccaa
     6721 accagccata acacagatgc cttgtggttc aatgaaaatc tttccctctt cgttcttttc
     6781 agatcccacc gcatctccgt aataatcgta agcgcgtcgg aaccattttc catcccagcc
     6841 tgatgcccag actgcctgtt ccattttttt cgccgcgtct tcatacttct gcgccagttt
     6901 ctcttcctgg cgaattctag ccatttgaac catctcttgt gcggcaagaa tataaagccc
     6961 ggcgatcagc acgctttccg cagtgacgcc atccttattg gtggtagttt gaaaagactg
     7021 ccccggtgtg tcggaaaaac aattgagatt caagcaatcg ttccaatcag ctctgccgat
     7081 caatggcaaa tcatgcggac ctaaccgatc gagcgtgtat tgcatgcttt tttgcaaatg
     7141 agaaaacaat gattcctcac tgtgcggttt gttatcgaag ggaatcattt cattcaaaac
     7201 ttcccaatca gcggtctctt tcagatattc tgcaaccgcc agaaccagcc aaagggggtc
     7261 atcattgaac ccaccaccga tatcgtcatt tccctttttc gaaaggggtt gatattgatg
     7321 gtacgttcct ccggtttcaa gctgggtggc ggcaagatca acaatccttt ggcgagcgcg
     7381 cagcggatca agcatacaga atcccaaaag atcctggctg gagtctcgaa atccgattcc
     7441 cctcccgatg ccactttcga agaatgaagc agagcgtgat atgttataag tcaccataac
     7501 ctgataagca ttccacacat tgaccatcct gttcacatgg atattcggag tggttgcatg
     7561 gaatatcgat agcagccttc gccaatgcgt tcccaactct tcgaagctac ggtctacttg
     7621 cttgctttga ctgtattttt gaatggtgga aacgaccgaa gttttattga tgagcaacga
     7681 atctccaaca aacttgtttt ccaccggatt ttcgtgatat cccaacaaga agatgattcg
     7741 cttttcttcc cccggttcaa gttctgcatg gatatgatga gagccaatag ggtaccagcc
     7801 tgatgcgatc gaattatggg tcttattttc ttccaccgcc cggggggtat cccatccccg
     7861 gtaagccccc aaaaagtctt cccgctgagt ttcaaaccca tcgatcggct gggaacaaga
     7921 gaaaaatgcg aaatgattcc tgcgttcgcg gtattcagtt ctatgataga tgacattgcc
     7981 ttcaacttcc acttccccga tcgaaagatt cctttggaaa ttcgtcgcat catcccatgc
     8041 atcccacagg caaaattcca cggcagagaa cagagagagg tcagcttttc tatcacgctt
     8101 attctgaatt cttacatccc aaatttcaag gttttctcca agcgggatga agtagcagat
     8161 ggttgaatgg atcccgtgga atgttgattc aattctggta tagccgagac catgatggca
     8221 ggaataatca tccaagggtc tcctgacagg ttgccatgtc ggtgaccaat atgaattgcc
     8281 ggcagaaatg ctcgcatcat tatcccgcaa gtaaacatat cgaccgccag tatccgcagg
     8341 cacgttattg taccgataac gagtaagcct tcttaaccgg gcatctttat agaaggcata
     8401 gccccctcct gtgttggaaa ctattccaaa aaaatcttca caccctaaat aatttatcca
     8461 ggggagtgga gtttccggtt tcgtaataat atattcgaga tgttcatcat caaaaaaacc
     8521 gtactgcatt aagcctcctg aagcaacgga tggtagattt tgctgctctg acgatttatt
     8581 aaaagttcaa attggtcaac gatcttcaga ctttccaaca ggctacaaaa tgatcttttt
     8641 ccacttcgac ataaactggc gtctctttgc acttctcaaa tgcataagga cagcgttcct
     8701 ggtaaacaca accaccattt ccctggggag aaaccacgct tgaatcttga tcagaagggg
     8761 ctttccattt accatccagg cgcgggaccg aagccatcaa catttgcgta taggggtgca
     8821 gcggatcatt gaagattttt tccgtgtttc ctttttcagt aatgaaccca tgatacagaa
     8881 taatggcatt cttgcttata taattggcta gtgataggtc atgggtaatg aaaaggactg
     8941 aaagtccatg tgcttgaagg gttgccaaca aattgagaac atcgatccgc accgaggcat
     9001 ccaacatact gataatttcg tctgcaatca acagttcgag ttccaacagc aaagcccgtg
     9061 caatcaaaaa gcgttgaagc tgcccaccgc taagctgatg aggaaattta ttcaacacat
     9121 gctgggggtt caaaccgaca tcttccaaag cggtgtgcac tttttcattc caggcgctca
     9181 tcatcaaatc tggatagaac tcatcatgaa tgacatggaa tatccgatca gctttaaata
     9241 tcggattgta cgaactataa ggatcttgaa aaacgccctg cactttgcgg tagtaattct
     9301 ttagctcttc tcccctcaaa gtagcaatgt ctttatcgtt aaaaacgatg cttcctgaag
     9361 tgatcgatag tagatgcaag atcattttac cgattgtgct ctttccactg ccactttcac
     9421 cgatcagtga aacgatctcc cctttatcga ttgtgaaatt gacccctctg acggcatgta
     9481 attctttccc tccgaaggtt ccaattcgat aactcttcga gacattattc aaagtaagca
     9541 tgctacattt ccctccagca agcaacatga tgatctggtt tgatttcaac gaatggagga
     9601 gtttcaatac acttttcaaa agctaccggg caacgctccc tgaagcgaca tccaagcggg
     9661 ggatttagaa gaagaggcgg ctgtccggga attcctttta agcgttcttc ggaatatttc
     9721 gctccgatct tgggcagtga agcgatcagt tgttttgtgt atggatgacg cggtgcatga
     9781 atgatcgttt cggtgctggc tttttctgcc agcaaccccg catacatgac cagaatcgta
     9841 tttgcaatct gatacaaaat ggaaatatca tgggtgatga agatgatgct tttacagcag
     9901 ccttcacgga gaaactcgat caacatctca accaccgctt tttgactcgc cacatccagt
     9961 gccgatgtga tctcatcagc gatcagtact gtagggttca ataacgtaga taaaaccatc
    10021 accattctct gtttcatgcc gcctgaaagc tcgatggggt acatcttaag aacatcatgc
    10081 ggcaggttca ccagatcgag ccgatgtttt atctctggca gtttttcttt ataatcaaca
    10141 cccttcgctg ctaacaattc cctggtcagc attccaattc ttcggatggg gttcatggca
    10201 ttcatggcat attgtggaat aatggaaata tcatgaaatc gaaagtcatt cattttttca
    10261 ttatcccaaa tgggcatttc agtgccattc agggttacgt gacccttgat atatttcatg
    10321 ggcggattca agaaaatcaa actctgacat aatgtggttt ttccacaacc cgattcgcct
    10381 gccaagccca tgatttctcc cgatttgacg ttaaaagtca catcttccag cgcatgtaca
    10441 tcccctttca aggtctggta gtaaacagtc aagtcttcga cgatcaaact cattttctac
    10501 atctccctaa gtttcggatt aaatacctca tccagaccca cattcatgct gtataaagct
    10561 ccaacaataa tggtgatggc taaccctggc ggcacaaacc accaccacat cccaaactga
    10621 agcgcgcccc atagaacaga atactgcatc atgagaccca gagagatcgc attggttggg
    10681 cccaaaccga tgaaatccaa tgttgcagca gtaaggattg ccccaccaaa ctgcaaaatg
    10741 aagaccaaca ataaatacga catcatattg ggggcaattt ccgaaaaaat gatctttttc
    10801 gatttcatgc cacttagcct tgccagatcc acaaagtcac gtgtgcgtag tgaaaatgtt
    10861 tgtgcgcgaa ctgctctcgc cgtccatggc cacgaggtaa atccgatcag caggcattcg
    10921 acaaaaacac tacgaacctg tagataagcg gcaatgatga gaagtaccgc catagtcgga
    10981 atgaccagga cgatattcgt aagtgcattg agaatttcat cgaccactcc ccctttatac
    11041 ccggcaataa aaccgatgat cagacctaac agcgtaccaa tacccccgcc tatgatacca
    11101 acctgaaagg tcgcgcgcag cccactcaca aattgggcat atacatcctg tccaaagaaa
    11161 gtggtcccca gccaatgttt tgctgaggga gccagagcgc cttgcggctc aacgtaatca
    11221 aatggtttat agggcgacag cgagggaccg aaaatactga tcaaaaggaa aaatataata
    11281 ataaagagcc cgatctttaa cttccaattt ctaaatgcaa agtacagggt ttcatttttg
    11341 tgtttttgct ttttcatgcc gtttctccgg tcatccccat acgtgtacgt ggatcgacaa
    11401 ggatgtagat aatatcgatg gcaaaatttg ctatcagcac tcctacgatg ataaagagga
    11461 aacagccttg aatgaggaaa taatcctgat tttgaattgc ggcgagcaaa agatagccaa
    11521 ttcccggata agaaaaaacg acctcggtca ccagcgcacc agccacgatg acccccaatt
    11581 tcaacgccag tcccgtgatc tgcggaagaa tggcatttcc gaaagcgtac ttcctgatca
    11641 atttcttcgg ggcacccaag gctcccaaat agtgcgaata atctgattcc aattcataaa
    11701 taatcaaatt ccgcattcca atggcttgcc ccccaaattc caccaaaaaa acagatgcaa
    11761 acggcaagaa ccagtgatgg aacacgctcg caataaattt caaggaaaag cttggttgta
    11821 aagagaaact ataagcaccg gcaataggga atattcccaa aacaattcca aagaaccagg
    11881 ctagcaatat ggctaaccac atatacggcg tggcattaag aatatagccg atgggcgaca
    11941 acgtattgtc tagcttcttt gaacgagccg caaccgctcc aaatttattc cccgcatacc
    12001 aactcagagc aatggacgga acaagcaaaa aaacatcata aggaacggcg cgtgcgagaa
    12061 cttttgttac cggggttgga aaaaggtaag tgcttatacc aaggtttccc tggaaaagta
    12121 gtttccagaa attcagatat tgctgcccta aaggcagatc caatccaaat agatgcgaat
    12181 agtaatcgta catcatttgg attgcagttg cattgctgct tacggatact ctggtgagca
    12241 tatttctaac cgggtctccg ggcatgaagc gaggaatcat ccaatcgatc gtgaccgcaa
    12301 ggaaaaaggt aatcaggtag atcaagattt ttcttaagaa atattttttc acgaagtctc
    12361 cttttttttc tggctattat cggtttacca gtacccctcc ctctagcctg cagggagggg
    12421 tatctatttc cagaaatgaa gagatgttga aatctattcc gcagtaactg gttccagatt
    12481 gatcagggtt tgcaagccac ctaactgcca ataaccagac caggttgtgg gcaaggtggg
    12541 agaagacgag ctttcatccg gccagttggt ccaggtcgag ttgctgtact gtgcccagag
    12601 tccgttgtac cataatggga tcattggcat atcggtcagc attgcgcgtt gaatcgctga
    12661 gcaagctgct tgcatgccct cttcatcaga catcgggatt ttcgccaggt tatcaaccag
    12721 atcgaagact tcttgatttt catagcgacc aaagttgccg ctctgcatct gctcctgaat
    12781 gggatgacgg aatagcagat tgtagagcgt ccatggtgtg ttgctcatgg aagcccagtt
    12841 gttcagcgtc atattgaacg ttccaccctg caaggcagta ttccaggctc cgtaatcagg
    12901 ataggcagat tttagattaa taccggctgc ttgagcgctg gaagcaatga tgtcaatggc
    12961 agccatccag tcggtccacc cgctggggca ggtaacttcc agttcaatct tttccccgtc
    13021 cggagtctcc acaaagccat cgccatcgat atccacatac ccggcatcag ccagcatttg
    13081 tttggcttgg gcagtatcaa aggaatatcc caattcagcc attacatcct ggtcgacata
    13141 tttgtccaaa accggcagca aacccgaagg actggcggct tgtaccaact gagcgtacgc
    13201 cacatttacg atggaaggaa catcaatggc aaatgctaat gcccgtctga aagcagcatc
    13261 atccaatggc ttcaacgtgg tgttcaggaa cagaaccgcc gtgttggcag aaagcatgta
    13321 aggagcatct gcaaagtaag tcttcacata tcctttatca gccatttccg caacaccggg
    13381 caagaagtta gtgctgagat cgagttcacc tttcaagacc atgcccaaag ccacgttgtt
    13441 gctggaagtg cgaatatcaa cgatatattt gggagccggt gcaccaaaaa catcaattcc
    13501 ccaccatgct tcattgcgta tccagatatt gcggtcttca gcatgtgaga gatatttata
    13561 ggcgccggag ccaatgggat tttcattcgc ccccacggtg atgtcttctt cagatttgct
    13621 ttcccagatg tgctttggaa cgatcggcac attgtacagg ttattttcga actcttgata
    13681 caatggttcg gcggaaagtt tgaactcaac ttccgcgtca ctcagcgccg ttacgctttc
    13741 tgtataagcc cacatcggac tgaaccacag ggcagcatat tgcgtgccca ggttgaaggt
    13801 gaacgctaca tcctcagccg tcaatggttc tccatcgctc caggtcaaac caggtcgaag
    13861 agtgacaacg aatgtggtat catcaaccca ggtcccgctt tctgcaagcc atggagttaa
    13921 ttcaccggtc tgtgggtcaa acgcatacag gaattcatag acaaaaccga ttgttcctgt
    13981 tgtgttcgcc agactacccg gctggaatgg attccatgtc gaggcgggac cccaagctgc
    14041 tccactcaca taaatggttt cattgcgtgg aaattcggtg ggtgccgctt cttcaactgc
    14101 aggtccctca gcagcgggtt ctgttgttgc cgcttcggct gcaggctggc atgctgccag
    14161 tacaactcca gcaacgatga aaatacttag caaaaacaaa atattcttac gcatttccct
    14221 ctctcctttt attgggttat taggatttat tgtttgaagt tctttctctg caattttatt
    14281 tttcttctat aagacctcct tttgttggta acgctcccaa cacgatcata aaaaaaagaa
    14341 tagtgtagcg ttcacaaaat tctattccgc gtggaatccc gtatgatcaa ttctgacttt
    14401 agaatcacct ggcgcgatgg tttcaaccct tcttcaacca ggctgatcaa cattttcaaa
    14461 gcttcaaacc cgaattttcc aatcggttgt cttaacgttg tcaaggcagg gtacaccgga
    14521 gacaccagcg ggaaatcatc atacccgata atcgaaatat cttgcggaac attgagcccc
    14581 gcttcgcgaa tcgcgcgtat tgccccaatt gccatctgat ctgacgctgc aaaaacggca
    14641 tcgggcattt tcggcaataa cttcttcatc cccaaataac cgctttcttc cgtaaaaata
    14701 ccttcaacga tcatttcttc atctaccggg atattgtttt ttaccaatgc ctgtttgtat
    14761 ccatattttc tgtcgattcc caccgttgta ttttgtgggc cagtgatggt ggcaactctt
    14821 ttatgtccgt tatccaacaa atattgcaca gcccactcta cagattctgt attatcgata
    14881 tcgacgtagc tgatttgctc cccctgaaaa ggtcgtccca aaaccaccag tgggatgcct
    14941 gaatctgcca acaaatcgat caaagtgtcg cccctttgat cagactgaaa aagaatccca
    15001 tccatgaatc cccggcgaga tacacgggaa aacatctgat cttcgtcttc tttatccccc
    15061 atgaggaaaa gggaaaacgt ataattttgt ttactgcacg cctgggcaat tccctggatc
    15121 aaacagggat aataagggtc agtgaagaag gaattgatgg ttcgtggcaa tatcaagccg
    15181 cacgtccagg tgcggttgga aacaagcgct ctggcagccg ggtggggctg atatccggtg
    15241 ctgcggatca cttctttcac atgttctctg acttttttgc tcacattagg gtggttattg
    15301 atcacgcgcg acactgtaga gcgtgagacc ccacttttct ttgcaatatc ttccaacgta
    15361 aggttattca aagattgcgc tccaaaggct gcctgatttt tcgataaaat tgggaacgct
    15421 cccaatttct acattgattg tatagttctt cctcagaaaa gtcaagaggt tggggatcaa
    15481 taaataccga aagggggcat ttcgggaagt aggaaaccgc cgggtcgcca ttggaaacac
    15541 catagcccca tgcgctttta acgttcaaga cgggagagct gattcgtctc ccgttccccc
    15601 ttgcaaagca ttattccgct tctatcaagg tcattttttc gataacatca ccgctgaaat
    15661 tcgggttctt gcccggatcg cggcgagtga tgccattgac cacgtccatc ccttcgctca
    15721 cctgcccaaa gatcgtatat ccaccattca aacgcggcag cggagcatag gtgatgaaga
    15781 actggctgcc gttggtgtcg cgcccggcgt tcgccatgcc gaccacgccg gcttggtcat
    15841 acttcagatc cgaatcttca ttcataaact catatccggg tcctcccgcg ccggttccgg
    15901 ttgggtcgcc cccttgagca acaaagccat ccagaacacg gtggaaggtt acaccgtcat
    15961 agaaaccctg gcgcaccaaa aacacaaaac tgttgaccgt gataggcgct ttggctggat
    16021 agagggtaat agtaaattcg ccccctttcg ccatcttcac gagcacgcta tattctttct
    16081 taatgtcgat ctgcatgggg ggaacggcag tgaattgttt atgggtgtct ggcatggacc
    16141 ttttcctcgt tttcgattgt tttcattatt ttaatataga tgttctgctt ccctcaacct
    16201 taaaacaaaa agaggatctt tttatgtgtc aaagaagcct cttttttcat tcgataaata
    16261 gtatttaata cttatcctca agctccttga tcagatccag acgatgctga tggcgaccgt
    16321 cttcataatc agcagcgagc caatcccaaa ggatcatttc agccagctcc tcacccacca
    16381 cacgcgaccc catgcacagg acgttggtgt tattgtgctt tcgcgaaagg accgctgaat
    16441 acggctcgct gcatacgacc gcacggatcc catggatctt gttggcggta atgctcatac
    16501 ccaccccact gccgcagatg agaatccctc gctcatattc tccggaattg atcttttgag
    16561 ccaccttttc tgcgtagagc ggataatcag tgcgggctcg atggtatgtt ccgcaatcct
    16621 ccacctccca tccctgtttc tccaagatcg ttttcaggtg ttcttttaga ggatatccga
    16681 tgtgatcact tccaatgacg atgtgcttct gcatagaatg ttctagcgat ggttgccagc
    16741 catgcgctcc caggtcagtc gacgcccctt cacagacagc gaaagctggt agatattttc
    16801 tggtaaggag caacccgata agcccgcatc cccaatagcg tggatatcag cgccggtttc
    16861 acgtgattgg agcgccagaa ttcgaatggt atccacatcc gccccttcca ctgaaccgtc
    16921 aatgaaagac atggtcatgg tatcaatgtc cttatggtga acatactcca ccaattcccg
    16981 gatcatatcc accgtgatgc cctggcgtga acccggcgcc gggaagtcga tcacatctgc
    17041 accggcgtcc atcaacgcat caatgaactc tctggcaggt tttttcgcca ttggatcgcc
    17101 aagaactttt tcgtagacgc cgtcttccca ttttcccgag aagatcaggg tctttccctt
    17161 gacagtcttg ccagccagtt tcgtagcggc tatgatgtca tcgattttcg cattcatgct
    17221 cggattcgcg cccaggataa tgaaatccgc tccctcttcc accgctttgc aaaagttttc
    17281 ttccgtcgca gttcgaccca atccaaatcc actcacatat aaatggtccg tctctgcact
    17341 cacacattcc aaataaacac ccaccgggcg attgaccagc ttcttgatat cgcccacatg
    17401 gtatgtctgc aagccttctc gatagttata atgtttcaca ccatacaggg gacttttctc
    17461 atcaaagcta taaccgttca acaataagat atccgctcca aaggcagctt ccaactccgg
    17521 attggtaaca ccgttcagca gggcattccc catggcgtta aaactctgtg ccagaatggt
    17581 gcgaccttcc gccaggcgga tagataattt cagatccatg ggggtcattt tttcaaaatc
    17641 acttctggtg caacttaata ttctctttac catgtttatt tcctcttttt tacaagaaac
    17701 gaaatgcgat acaattaaga tgtgtgagtg ggtaggttgt cgtgctattg ggcacttcaa
    17761 cctaccccgt gctttttaat actagtgaag agctattttg cttttatgtc agattctttc
    17821 gctaattcac ttttctcata cgctttaaag aatggtgcat agaccagaat tccgatcaat
    17881 gtgttgatga taaccacaac caccatcttc caatcgccac cggttcccag gagagccagg
    17941 acaggtggag gggtaatcca ggggagtgaa acatagaaac gcgaaaccag acccaatgcc
    18001 ataactccat aagtgaagaa agccgctaaa gtacccgaga ggatgaatgg gaccaacaaa
    18061 taaggattga aaacgaccgg cagaccaaat aagatgggct caataatagt aaagatcgca
    18121 gagggtaatc ctgcggtcgc gatcgtgcgc agtttcttgg acttggaacg gaacagccag
    18181 tacatcaaac cccaaataga agctgtccaa ccgcacattc tttccgttgc ttgaacccaa
    18241 atgtatggga gtttcgttgc cgccacacca gctactgccg catccgcatt ttctataatc
    18301 catgcgctgg tcagcggtcc ggtgataccg gtaaggatcg catcagcgtg cagacccact
    18361 gaccagactg cctgacccag caagaaacgc agcgtgaaag cgccaatgct atccacagcg
    18421 ctgaatatgg gggcaagcaa cttctgaaca gcagtaacca ggtcaaaatt gaagaccgat
    18481 ttcacagccc acataagcac caagacgaga aaagatggga tcaggctgga gaaggtagcg
    18541 gcgatctctg gtggaacacc gtctggcatt ttgatcgtga tgttcttctc tttgaacaag
    18601 cgcaggatcg acattgccac cagggaactg ataatggcag ccacaattcc ctgcgcaccc
    18661 agattggtaa ttgcgatcgt tccatcacta atcgtgctgg tcaaaagaag gaaagcagcc
    18721 agacctgtaa gagcactgga gagaagctcc atcccatact gtttggcata ggcaccgctg
    18781 atggcaacag caacgaacat accggtcaat cccatggtca actggtaacc gatcagtaat
    18841 ggaccagcaa tgggaagcag gaaggagaag aaagaatagt tggtggccaa accgaacagc
    18901 atcaggaaga tactaccaat ggtcaacacg ccaacggtca gcatcatacc acccgagatg
    18961 gttcttacga ttttgttttc accgatctta gcaaaaacag gacccagcct ggtatcgatg
    19021 atttcaaata tcttttcatt aaaacttttt ttctttgttt gcatttcatc ttcctttcat
    19081 agattgtttc caatcgaaaa tacgaaattt atactcgctg ccctgctgtg atcacctcac
    19141 tttctctttt aaaatatcca ccaattcatt gatcaaatta atttctgaac tggaagtcat
    19201 catggtatcc atagcatgcg tgaccaataa ctgttcattg atgcttttaa actgattctc
    19261 gattcctaat aatttcaaat gagttcgatg acttagagtg agtttttctt cagcagattc
    19321 gagaagtcgc ttggcttcct caaaatcgcc cctttttgcc gcatgcagtg catgatacgc
    19381 atcggctttt gcatcccccg cgaaactgat catttccatg gcgatgatct caagggactc
    19441 atgcattatt tcttcccctt gtgcaattcc agcgcctgat cgtaaatctc ctgcgccata
    19501 gctctaccat aatgaaccat atcaataaaa ccgtagggaa ttccttcctt ctccgctgct
    19561 gccacaattt gatccttttt atagcgaagt tgaggaccga tcagcagcac atcgtaatcg
    19621 ccgatttctt tgattgtgtc catgccaata ccgtaatcaa tgacggtgcc ttccggcgcc
    19681 agtttgcgca ttctatccac cactaaactg ctggacatac ctactgcaca gataattaag
    19741 attttcacga accctccagt aatgttaaaa tatgttaatc tatgagatta gttgtgaata
    19801 tagcatactt ctattgcatt gtcaacgata taatgtataa ttatgtgaaa actaacacaa
    19861 ggtcgaacat gctaccgatt caaaggcaac taaaaataaa agaatttttg gtagaaaaag
    19921 gttttgcgag cgttgaggaa ctcagcaagc aatttgaagt ctcagaaatg accatccggc
    19981 gcgacctgga cgagttgcac gaacaaggcg tcattcagcg catttacggc ggtgccatgg
    20041 ataacgacca ggctttcttt gaaatgtctg tgaatgcgaa agctggaaaa ttcgccacag
    20101 aaaaagtaaa aattggacgt gcggcggcgg acctggtgca aaatggtgat acggttttca
    20161 tggattctgg aacaacaacg ctggaagtcg ccaagaacct gaaaaacaaa aaaattacca
    20221 tcatcactac cgcattgaac gttgcagtag aactctcagc ttcgcctgac atcgatattc
    20281 ttgtggcagg cggcattttg cggaaaggtc cactgaatac aaccggtcct caaaccgatg
    20341 cctttatccg ggagttgcgc gttgacaaag tgttcatcgg cgttgagggg gttgacctcg
    20401 aatcaggcat cagcgttccc gaccccatta acgctcacaa taaacacacc atgatcgaga
    20461 tcgcccgaca ggtgatcgtt gtggcagatc attccaagct ggggcgtact acgaccagca
    20521 atattgcgcc tctcgataag gtgcatgtgg taatcacgga caatggagcc gatcccgcaa
    20581 tcatcgagga attgcgcaaa aagacgcagg tcattctggt ctaatttttc tttcatggca
    20641 cgctcaccat tcaaaaaagg tcgtattttg gacaggcaat ggagtaagaa tgaaaaataa
    20701 catcgcgttg ggtgcttcgt tgatgtgcgc gaattacctg aacctggagc atgacatccg
    20761 cttgctcgaa gagaacggca tcgattacct gcacttcgat gtaatggatg gtcattttgt
    20821 tcccaacttt ggcttaaacc tcgacctttt gcaaaaggtg cgcaagatca ccacattgcc
    20881 catcaatgtt cacctaatga tcgaagacgc ctcgcactat attgacacct ttatcgacgc
    20941 aggatgcaat tcattatcgt tccatcacga agcgaattat catgttcaag ggttgcttca
    21001 aaagatcagg gatcgtaaag tgaaagcagg catcgcactt aacccggcaa cgtcgttaag
    21061 cagtttagaa tacatcctga acgatctcga ttttgtgctc atcatgactg taaatccagg
    21121 gttcgccggg cagcctttga taccggcaac tctcgaaaag atcgccgatt tgaaaaggat
    21181 catccgagcg aaaaagctgg cgattgacat ccaggttgat ggaaatgtca gcttcgagaa
    21241 catacccaag atggtccaag ccggggcgac catgctggtt ggcggaacct cttcgctttt
    21301 tctcaaagac ctgtcgattc cggaagcagt acaaatcgtt aacaatttgc tttgaaaaga
    21361 tgcaaaaatc acactccaga cccatcgatc tggattgagg aagtttgatc gttgggattt
    21421 atttcatggg tacccatatc ccgcctgtac cagcttgctg ggcgaccgcc cgtccgagta
    21481 aaaagcgaga tcctttttat cgctgaagcg cggtgaccgc accgcaacac ccccaatata
    21541 ccgcttctct gatggtttca acgcacgatg gatctctttt atgcacatcc ctgcatgaat
    21601 atcaagccca aaagactgcg agcgtatgaa caatcatgtt cgcgaagcag agccgcccgg
    21661 aattcatccg ggttttgaag cctccaggat ccagttggtg ggtatttcgc tggaaaatga
    21721 taagataata gggtgaagga aatacgtgat tcaaaggaac gataatggat actatcaagc
    21781 ggaaatatgc aagctttatc ctctccaact tcccaagagt tctctctcaa attgatcgcg
    21841 atcccgattc tccaacattt ggctgctgtg atcgaaatta ctggcattac aaaatccgcg
    21901 atttttcttc agccatcctg cagcaatcgg ggttggtttt agcaaagctg tatcatatcg
    21961 actttccgga aaatccgctc tttcaaaacc aaaatgctgc gcacatcgct ctggctacgg
    22021 tgaacttttg gaaagagatc caattagggg atggttcttt taatgaatac tatccacacg
    22081 aacatggctt tcctcccact gccttttcct tctatgcagc ttgtgaaact taccgcgaac
    22141 ttggattgga cgagtccgct tatttggaag catttgcaaa aaccgccaca tatctatcaa
    22201 agcatgtcga atcacaagcc agcaaccagg aaatggcttc catcacagca ctgtacagct
    22261 attacctcat cagcaaagaa caagctgtaa agcaagcggt ggaaagaaag ctggagcgaa
    22321 ttctctccat ccaatctgct gaggggtggt tccctgaata cggcggagct gattttggat
    22381 accagagcgt gtgtctggat atgttggctg aatatttcca cctcagccat gacgagcgcg
    22441 tgctggaacc catgcagcgg gttgtcgagt ttctgaaatg gtttgtccat cctgatggga
    22501 gcatgggtgg agagtacgga tcacgaaata caatctattg cctgccgttt ggcattgagc
    22561 tgatgacagc tagaggcgat gcggctgccg tcgccatgcg ttcggcgatt ttcggcggtt
    22621 gtgatcagcc cgactatttc caaaactcca ttgatgaccg ctatttttcg cactacgttc
    22681 tgcattcatt tgtgcgggct tatgaactcc ccggtgaaaa tgccggaaca aaaatcccat
    22741 tgccctacca ggaaggggga taccgctatt ttccagatag tggcttgttg agcaagacaa
    22801 cgaagaatta tttcgcgatt tcttcgttga aaaaagggtg cgtgaaggtc tttcaagcag
    22861 atcatttttc gtttgcggat tttggatatc gaatcgtatt aggaaaaagc actctcggag
    22921 cgaccaattg gttggatgaa aagaacgagt attcttacca tgagaacgag aaccgtgctg
    22981 tgatccgcgg aaggttcacg aaggtacgta tccaaacgcc ttcccctcta aaccatgccg
    23041 ggttgcgctt ggcatccttc ttgttcggca accggttgat cggcttttta aagaaaaaaa
    23101 tgatcttcat cgacagccat cttgaggttc ggtttgagag aactattgaa tttcaagagg
    23161 acaaagtttt gatcaaagat aaaattgaat ctcccgaaaa ggtcaccatc aaaagcgcac
    23221 ctcccttctc gctccgccat gttgcatcaa gcaagttctt ttcttccagc gatcttgcag
    23281 aaagaggtga agtcttgctg aaggaaatca aacaggcgat gatagagatc gaatttgatg
    23341 gcaaaaccgg gcagaccaac attttttcta caggagaata aagatggatc ttgacgaagc
    23401 catcaaaggc agacgcagca ttcgcagata ctcagaacga gacattgacg aagaaacgct
    23461 cgaacagatc atccaggcag gaatgtgggc gcctaccgca tgtaatgatc aggattatcg
    23521 cttcatcatc attaatgaca aaaacaaatt tgacgagctc cttgcttatg gggctgccac
    23581 ctttatcaag gatgttcata ccgcaattct ggttctctat aaaaatacca gcgccaacca
    23641 ggaatacaag gattattttc aaagcggttc agctgtcatt caaaccatgt tgctcaaagc
    23701 acactcgtta ggtttaggaa gctgttgggt ctgtaacctg ccgtcgcaaa agaacatgcg
    23761 ccatattttc aatattcctg caaactactc gaccatcgca ttggtcagca tgggatatcc
    23821 gctttcaccc ccaaagccgg tgcagagaaa gcacaaaata aatgacgtgg tatgcctgaa
    23881 cgcgtttggc ttcaataatg aagagaacga ccccactatc gaaagaaaaa agtatctaaa
    23941 acgatttctg cgccgggtgt attaccgatt gcccaaaagt gctttcatca ggagatgggc
    24001 agagaagttg gaaaagaaat tcgttaatta acgatctccc tgattggcgg gagacgtctc
    24061 tattccatcc ttccttttat acactgccaa ctgattcaat ccgaacgaaa aaagatggta
    24121 atggataaga ttcattttac attccctgga caggtctgtg agtgcctttt ggttaaagag
    24181 catcttatgt tcatgatctg cttcgatact gaacaatccc aagcggctgc cgatggaatg
    24241 gattcctttg gtcgaaacgg caggagttgt gagaataatg gatgaccggt ttccggaagc
    24301 caggtaggat tgaagcaaag tcatatacga tacaggatcc gaaatatgtt caattactgc
    24361 gagcagcacg atcgagttga acttttgttt ccggtctggc agttcatggc tgaagctgtg
    24421 tgtgggaaat ttttctctgg cgtgtgctat ggattgttcg tcggggtcat atccccaata
    24481 gtcttcggga gaaaaatgag aagccaattc tcccgacccg catccgatat ccaaaattct
    24541 cccttcaata aacggaagcg cttgacgtat ccgcatagag cgcaagaatg gggacaaaag
    24601 accttcgctg atctgatcag acacagataa ttccctttct atgaatcgtg aaaaatgaaa
    24661 atcttcgcaa atatcctatc aaattttctt cttttgagcg atgagaataa atcaacaaga
    24721 tagccatcat atagaatgct gctttttact ccaaaatctt ttccagtacg cccagagaca
    24781 tgctctcaca aatctcttct acaaatgcaa tatttttggg cgtatcgctg gccaatcctc
    24841 cccgcagaat aataggaaaa agatccacat cctttgcgcc atggtacgtt gcgaaaacgc
    24901 aatattcggc gcaaaaacca cagagaacga ccgtatcaac attcatcttc ttcagttcat
    24961 cgagaagtgg agttttgaca aaagcgtttc catgggtttt ctggatgtgc agatcggcag
    25021 agttgatttt aaggtcatct ggcaggtcaa aatcagggtt ttccggaaca agtccattct
    25081 ccttgttcag atgttggatc gcaatgacgg gcaattgttt cttccgaaac acttttatgg
    25141 cttcgttggc atattcaacc gcgtcctgca aagagcgagc ggtttcatca ctggttctat
    25201 agaattgttt ttgaatatcg atcaccaata aggcaggttt catcgtgccc tccctttcca
    25261 tttgatacta gtataaaggg atatgccggc agaacgcaac cggattccag cacaatactt
    25321 gttcacagga ttttttaaac aaaaaagccc accaagtgta agtggacttt tcagcgggag
    25381 catgctatta ccaatctttc gccatggctt cctttttcat attgggtagc atcagttcaa
    25441 tggcatatcg caacgcggtg cggggcatat tttttttgtg ttccatgaca aattggaaaa
    25501 catcgtcttg gtggaggcgg ctggcttctt tgagcatcca gccgtaaccc ttttgcacca
    25561 tatcatcacc gtccgtcaat acgatcaagg cgatctcaaa aatatcctgc aaatacatgc
    25621 ctttacgggc aggaatgata agcgacactg ccgcagctcg tttcaaccat cgctggtccg
    25681 tacgagccca ggttttcaat tccgctatgt tttgtggaaa tttcatgacc aagtcgccaa
    25741 ctgtatggtt acaaaacccg tcgcacttcg cccaattatc aatatacttt tcgatccaac
    25801 cttcgaaagt gtggatgtcg ctggcttgaa attcgttgtt caattttgga agccaacccg
    25861 atacgatata tgcgtcttca caatagccgg ttgacagcaa ttgctcgcat agggcaaaga
    25921 ttgctgtttt gctgagatgg cagatctgtt tccagtactg atcgccaatc tttccaacca
    25981 atgcgacatt caccccataa catttcaccg gttccttgaa gaaccgttgg gaacttgctt
    26041 tcgttttttc atcagaactg ttttgcaacg tctgacgcac gtcttgaaca atggcttcca
    26101 tggtagcttc ttattttaac ggaaatcgaa tctctgtttc tggtttgatc gaaggatctt
    26161 ctccgggatc gttgagatag tattcataag caacgccggt tggttcaaag ccctgctgtt
    26221 taacgtaagc agtcaacgct tcataagcag gttccaccgt atcatagctt ccaacgtgca
    26281 gggtgatacc aaatttgcca ccagcgatca tgcctgaatg aatcttgtcc ttcgctggca
    26341 gcgaacgggc tactggaaaa ccggcttcta catccagtgc cttcatatcc agattgtaat
    26401 atgcggcgaa gggcatcccc accggctgct cccccagctc ttccagatat tgcataatgc
    26461 tcccataggc ttctccaaag aaagccggca ggttttcaac cgctgtgatc gtacgcacca
    26521 ccagcaccgg ttgatctttt gcttcaatga ttcgacaatc atatttcatt gtatcctcct
    26581 catatagaac ctattttcta ttttagccgc cgcagcacga tctgtcaacc tacaaatgca
    26641 ccaggtataa gttcctcatg tacaattaat ttcagaaatc tttcgttcgt actacatgga
    26701 ggaaaatcat ggaaacaatc tggttgacag aagttgggat cacgcaattc ttccaaaatg
    26761 caggggcatg gttgctgctg ccgatgcaac tgatcacttt tttagggaac gagcagttct
    26821 acatgttggt catgcccgcc ttgtactggt gcgtggattc ggtgctcggg ttgcgggtgg
    26881 gggtcatgct gcttaccagc ggcattctca atggaacggc taaattgatc tttaaaagtc
    26941 cgcgccctta ctggttcgat gaacaaattg ccgcccacag ttccgagagc tcattcggca
    27001 tgccatccgg acatgccatg aactccataa gcgtatgggg attgttggca gcttctattc
    27061 ggaaaaagag cgtaaccgtc atccttgccg ttatcatctt cttgatcggt atctcgcgca
    27121 tctttttggg ggttcacttc acttccgatg tgctggtagg gttgctgctg ggggcattat
    27181 tgctgattgt cttcttgcac gttgaaaaaa aggtggctgc ctgggtcagc aggttaactc
    27241 ttggacagtt tgctgcgatc atgttcctga tctcgttagc catcatcgcg atcactgcac
    27301 tgattatccg tgccaatgtt gattggagga tcccctccgc ctggatcacc aacgctatgg
    27361 aggctgcccc tgaaggagag atcgatccat tttcattgga tggcaccatc acgaatgcag
    27421 cagtgctgtt tggattggca atcggcgcag tttggatgaa tcggagaggc ggcttcaatg
    27481 ctggtggggt ctggtggatg cggctgctcc gttttgccat cggagtaagc ggcgtgttgc
    27541 ttttctgggc agggttagga atggtatttc ctgattctgc tgacctgttt ggtttcgcct
    27601 tgaggtacat tcgctacgct ttaactggct tatggatctc ggcaggtgca cctttcctct
    27661 ttgtcaggtt caatttagca ggaaaaaaag aaacaaaaga agggtagctt gcaaaccctt
    27721 cttttgtctt gtctactgtc tgaacgccgg actctcaatc gtttttaatc tcttgaattc
    27781 tggctctttt cccttcgatc tgggcatcga ttccaaggat cttctttgcc aattcttcaa
    27841 tagacggatc cagggcgacc cccttcgcca tcaattcgag cgccttttct ccgatttcca
    27901 ccttgagccc tttgatctca tcttctagct tgccgatctc ctgattgatc tcaaaggttt
    27961 tcacggcatc actagctttt tcgctcactt ccttcagacc cttgcctgcc tcatctatcc
    28021 cttttttcaa tcctttcgaa agatcatcta aaataggcac gtctacctcc acagtattgt
    28081 tatgaaaaaa gagtacaaca caatttcaaa aagtcaagaa cctcaagaaa gaatgtgctt
    28141 gcattacgga ccgcacaatc aatcaacagt actttttcct cgagtacttc atgaaaaccg
    28201 tcgtctcaga cagcatcaac gctgcatcct tgcaattatt cttgcgctga agattccagc
    28261 gttttggact tgccagcgtt cggaacaagc cgcaacaaaa caacgccggc aaggatgagc
    28321 atgccgcccg ccagttcgat ctttccaagc agttccccaa agaccagata cgcgaaaagg
    28381 gaggtgaaaa ccggttctga tgtggctatc agattagcca cgctggcagg gagcgcctgc
    28441 aggctgatat tgtaaagacc gaatcccatc acggtcggtc cggctgccaa caggaacaaa
    28501 ggcagccaac ccttccaatc cgtcccgagc cagagcagat cagcgggttc ttgagcaatt
    28561 ccggggatca tcgaagcagg gatcagattg atcgccaaca agcataaccc tgcaaatgcg
    28621 aaggtgtaga aaattgtggt ccacgggtcc atgccatgtt gtgctgcatg acgacccatc
    28681 aaactatatg ccgcataact taagccagcc agcacgccgc tcacgatgcc aacgaggttt
    28741 gtttgccagg catctcccgc atacgctcct gaaaccagaa cgcaaccagc cagactgcag
    28801 acgatcgcta cgatctttac ccaacccagt tcctccttca aaagcaagtg ccccaacaaa
    28861 gcggtgaacg ctgccgaact gtacgacaac accgttgcca cagaagcccc attcaatgaa
    28921 acagaaatgg tccaaaaggc attgaagacc gccaaaacca agccatacgc caataaatag
    28981 ggcgcctgtt ttctgtttaa gagaagcaat ttcttttgct tgaaaagcag aatcgggaac
    29041 agcgtgagaa ccacgaagaa atcacgccag aaagccagca ccagggcagg tagcccatac
    29101 tgcacggtca aatagcgaat aaagatagcc gtggttgaaa gaaaaatcgc gctggctaat
    29161 gcaataaaat aagcctgatt tgaagttggt ttcgacattg ggcacccaaa aagaattttt
    29221 aatcagcgta tgattatatc ctcgttatga gtgaagaatt gttttcctat aaagggatac
    29281 gtgaaatcac tcttcgattt catagggaaa tctattgact tttcgttgaa acagtttata
    29341 atctcgaatg tacaatattc tgtattgagt atatttatgg aaaatgatct cctcagacca
    29401 ctcatttctc agcagggcaa agtcgtcatt gccctggcta agatcttcct gaatcacaag
    29461 cccggagaaa aaattgagcg ctatcaagac tatgccaaac ggtttcaggt cggaaccggc
    29521 accgttcaag cggctctcaa gtatctacag gacgtgaggg cgataagcct ttccaccaag
    29581 gggcatctgg gcaccttcat cgacaagatc gattatcccc aattatgcgc actggcgggg
    29641 caggatatca tcgtgggcgg aatgcccctg ccctactcca ggcgatatga gggacttgcc
    29701 agcggactca acaaagcgtt cgcccaggcg aacctgggga tcaatctgct cttctgtcgg
    29761 ggttccatga accgcatcaa gactttgatg caaggtcgct acgatttcat cgtcctctcc
    29821 aagtacgcct gtgattctgc caaagcaatg ggatggaaga tcagagatat ttttgatctc
    29881 ggcattgaaa cctatgtggg tcagcacgcc atctttcttc gagatcaaag caaacaatcc
    29941 atcgaaaatg gcatgaaggt cggtatcgac cccgattcca ttgaccaaat caatctctgc
    30001 aaagaagtga ccaaaaatct caaagtggaa tttgtggaaa ttagctacat gaacctcagc
    30061 tcagccctgc gcgacaaaca gatcgatgca accatctgga acaccgatga ttttgccaca
    30121 tcagattctg ctttcaagat catctcgctt gaaaagaatc agatcacgca aaatctggat
    30181 aacaccgtcg ccgtcatcgc cgtaaaagaa gatcatcaat cattgatcca caccttccaa
    30241 tccaccatcg acgttgagct cattttagat atacaaagaa aagttatgag taaagaattg
    30301 cttcccatgt attagttcct ggagagaaag tgccattcac tgaccgtttt aagtttctgc
    30361 ttaaaagtaa acaagcatcg ccagcatcca tccgtattac ggaagagctt gttgatttaa
    30421 tcgaaaaaga gtaccacaca aaactggacg aatccaatgg cgcatccctg gtaacccacc
    30481 tggcgatcac gatcaaaaaa ctcactgccc atgaaaaatt atccaaaatt ccggacgtct
    30541 gtttgacgga agcgctctcc ttcaaaaacg aaatgaaatt cgccaaccga ttaacaacat
    30601 atttaaaaga aacccatcaa atagatttca accgaagtga aaccgcattt ctaaccattc
    30661 acttacgaaa cgttagtcaa catttggaaa ggaaggagga gaaaaaacga aactaatttt
    30721 cagaaaacag gaggaaggaa tatgatcaag gtcaatattg gtggaatgaa cgcacaagag
    30781 atcaaagatc tcatcgacaa attcgccgaa gggaaaatcg aagcaaagat cacatccgat
    30841 atgagtggag cgcaggaagt tgcgcaagga gaagccgatt attattttgg cgcatgtgcc
    30901 accggcggcg gtggcgccat ttccatggcg atcgccatcc ttgggtactc ccaatgcttc
    30961 accgccagca tgcccggcaa accaccccgt aaagaagaaa tcgaaaaggc tgtgcgggat
    31021 gggaagaaag ccttcgggtt cacatgtgat catgtgcagc ttgccgttcc attgatcgtg
    31081 caagccatct cagaaaattc ttcggcaaaa taatatggag gaataaaaat gtccgtaata
    31141 aaaatcgttg tagtagccat cgcaggcgct ctcggttcgg ctctcgcgaa ccgaggaatc
    31201 gcggtttacc atgatgggct cagaccgatc atgcccgaat tgatcgaagg gcgcatgaag
    31261 agagccgaaa tggctgccat ctcgtttggg ctgagtttcg gtctggtgat cggattcggc
    31321 atcccattct cgctcacctc atccattatc ctcgtccaca gcctgtggct tggcacagac
    31381 atcattggaa cgtggttccc cggttctttc gaaaaagggt ggatgaaaga caagaagagc
    31441 ctgtggggat tcgtcgggtc tctggtcgcc ggtggtttat atggtggatt gctggtcgtc
    31501 ggcttacaag cgatcgtaac gttgttcgag aagctcccca tcaacttctt cggctcgctc
    31561 ggttcattgg gtgacccgat tctgtatgcc ttcgccatct tcccggcaat cgccgttgcc
    31621 atgcagtttg gtattctcaa aggcatcatc accttcatac tgaccatcct ggctcgccag
    31681 atcgcaacag ccgcaggact ctccagcgct gatggcattg ccctggcggt aggcattcta
    31741 ctcctgatgg gctttgcaat cgcagataaa ccaaaaggag aaaagaaatc acagccaagc
    31801 atgcaggcac tgtttaccga gcgcgcaaat cgcatcaaga gcaacctgcc ctatatcgcc
    31861 gtcatgggcg ctgtctatgg cattgcctgc aacctggcaa ttatgatgga aggaccacaa
    31921 tccatcaccg cattggcagc aggtgaccgg gcagcagcaa ccagcatcag cgtggcacgt
    31981 gccatttcct tcatccctct gaaagcgatg acctcactca cctcaggtgt cttcgcaatg
    32041 gatggatttg gttttgtcgc aacagccggt ttgctttctc ctagctggat cgtcgccgca
    32101 gtggccggtg cagcggtgat gagcctggaa gcttacagcc ttgtctttat cggcaaacta
    32161 ttggataagt tcccaggcgt gcgcaacgca gcagataata ttcgcacgtc catgacaaaa
    32221 ttgcttgaag ttgctacgct ggtgggttcc atgatcgcag cccataccat ggctcccggg
    32281 cttgggtttt tggttgtcgc aggtgcttat gtcctcaacg aaatcaccgg aacgcgcatc
    32341 gtacgcatgg caattggacc aatcgcagcc atttttacag gcattctgat caacatcctg
    32401 gctgtggttg gattgtatac cccgccagtg taggggaaaa actccttcct tcaacttatt
    32461 atgaagacat tcccaattga ccaaataaca gtagacaagg cgaaacacat gcagtttcgc
    32521 cttgtcgaat gcatttctca ccattttgac ggtgaatcca ttctgcaggc gggggattat
    32581 ggcgtgaacc aaaaaacggg acgccctctg catactgcgc gagcagaaaa agtgttggcg
    32641 gaatttttcc acgcagaaga cgcctgcctg gtacgcggag cggggtcggg tgccattcgt
    32701 tcattattca tggcgatggc aaaacccaac caagaaattt tgctgcataa agcccctatt
    32761 tacccgacca cagcggtcac catccgcgcc atgggtttga aaccggtttt cattgatctg
    32821 aaccatcctc aggagatcaa taaagccgac gttgcccggg cgcaattggg catcatccaa
    32881 cacgcccgtc agcaaatgga agatcgttat tcgctagcaa cttcgatcac atccttaaaa
    32941 aagatcaacc cttcgatatg cctgatcacg gatgagaatt acctggtgtg caaggttgag
    33001 cgaatcggta tcgaactggg ggcagacgca tcaacctttt caacctttaa attgtttggt
    33061 cctgaaggtg tcggatgcat tctcagttca agaaaagtga tcgatgcgat cagaaaagac
    33121 ctttactcag gcggttcaca ggtacagggc gtggaagcgt tggacacatt gcgttccatg
    33181 gtctttgcac cggtagcttt tgccattcaa aaagaagtgg tagacgaaac cgtaaaacgg
    33241 atcaaaaatg agaaaatcgc cggtgttcgg gatgcatata ttgccaatgc ccaatcgcgc
    33301 gtgatcctgg tggagtttga aaaatcgatt gcgaaaaaag tgctgcaaaa cgcagtgaaa
    33361 ttaggcgcag cgccccatcc tgttggatct gagtcgagat atgaaatcgc cccgatgttc
    33421 tatcgcgtgt ccggcacttt cctggtcgac gattcatcat tggctgacac catgatccgc
    33481 attaatccca tgcgcagcgg ttctgatctg attatggata tcttgaccaa gtcgatcgag
    33541 caggcaggat gaattatgtt tttagaccaa attcgaaagt acaatcctga tttattatca
    33601 gttgccctgc aatttcaccg ggcagggaaa ataccgccca gttgcttcgt gattgatctg
    33661 gacacagtca tagaaaacac cagagccttg gttcagaccg caaagaaaaa cgacctgacc
    33721 ctgtttttca tcaccaaaca ggtcggcttt aaccccgttt tcgcgaaaga agtatggaaa
    33781 aatgggatcg caaaagcagt cgctgtggat tggaatgagg cggtcagctt aatcaaaaac
    33841 aagatcccta tcggtcacat cgggcacctc gtccaaattc cagattgttt tatggaaaca
    33901 atgcttcgcg cgaatcccga ggtgatcaca ctattttcaa tcgaaaaagc gcgcaagtta
    33961 tcgcaggtgg ctcagaaaga aaacctgcca gcgaatgtgc tgctgcgggt tgtagataac
    34021 ggtgactttg tttattcagg tcaaacgggg ggcattcccc tatcagcact cgaacgcagt
    34081 cttgacgtgc tgaacacttt tcccaatatt cgaatcgctg gggtaaccgc ctttccctgt
    34141 acagcctttg accccttaca aaacaagttt gtgccaacaa aaaatctgca tacgctgggt
    34201 aaagcagccg agatcatcgc caggcaatac tcccacgccc ggttgcagat caacgcgccc
    34261 gggaattcgt gctgcgatac gttccccctc attcaaaggt acggggctac ccatgcggag
    34321 cccggtaacg cattaaccgg cacaactccc ctgcatgccg taagcagcca accggaaaaa
    34381 cctgcgctta tccacgtcac cgaaatatcc cacgaatata aaaatgtcta ttattgtttt
    34441 ggcggtggac tatatcgccg ttccaccgtt accaaagctc ttgttggaaa cgaaccaact
    34501 gcccttaaag aagccagggt catttctcct gaaaaaacgt ccatcgatta ttacgttggg
    34561 ttgaagatga gtacgaaaga gagggcagaa atcggagatt cggttctctg gtcgtcgcgt
    34621 gcccaactgt ttgtaacccg ttcttcggtt gccattgtaa aaggaatccg gcatgggaag
    34681 ccggaactgg ctggcattta caatccactc ggttggaaaa ccgactttta agtgtgaaac
    34741 aatgaaacgt gccgtattac ttgtacttga tggattgggc atcggtgcca tgccagatgc
    34801 tgccaaaacc agaccgcaag agcagaatgc gaacacgctt ggctgtatct taaaacatag
    34861 accgcatctc catatcccaa ccttgaccag aatggggttg atgcattgtt tgaaccgtgt
    34921 tcaagatggt gcagcggagc tgctggctag ctggggatgg tgtgaactgg cataccgggg
    34981 agccgatagc taccttgggc atcaagagat catgggggtt attccgcttg atccgcaaag
    35041 aacgttactg cgggacgaaa tcgatggatt tgaaaagatc ctgaccgctc gcggttttca
    35101 ggttacacga ccacttgccg ggaaacccct gttgtgcatt aacgatgcaa tcatcgttgg
    35161 cgataatctg gaagcagatg ccggacagaa catcaacctc accgtctgta ccgatagaat
    35221 cgagttcgga aaagccgtag aaatcggcaa aataatccgc agcgctgtac gggtggcgcg
    35281 cgtgatcgtt tttggcggtc ccgggttgac tctgcagaga attctggaac atgttgagca
    35341 aagagagaat gggcaggtgg gtgtgaacag cccggcgatc ggggtgtata acgaacatct
    35401 gctcgtcaaa catatggggt ttggagttga cccgcaattt caagcagcat ccatcttttc
    35461 acaagcaaaa aaagacgtgt ttctggatgg caaaatggct gacctgattg aatgcgagca
    35521 ggcagtcaaa agccccatcg ttgaaacgca agcattgcta gattgcgtac aaacacaatt
    35581 ccgatcactc gaagatgggc tgattgccgc aaccgtgcag gagactgatc tcgcagcaca
    35641 tggaggagaa atcgaccgtt tcgcagacat cctggaattg gtcgaccgtt tcttatgcgg
    35701 ttttcttccg caaatgaaaa aagaagacct gctggtcatc actgccgatc acggcaacga
    35761 tccgggtttg agaaccgggt tacacaccag ggaagctacc ccgctactgg tattttcgcc
    35821 ccaatttcgc cccacaccgc ttggtaaaag aaaatgcctg gctgacagcg gagcgtc
//
LOCUS       DAOSDS010000012        34178 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_640_length_34178_cov_3.987574, whole genome shotgun sequence.
ACCESSION   DAOSDS010000012 DAOSDS010000000
VERSION     DAOSDS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 34178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34178
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_640_length_34178_cov_3.987574"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="PLL88_02760"
     CDS             <1..729
                     /locus_tag="PLL88_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00515.1"
                     /translation="WVIAMSTNIFKKLFLLARPAAGKSEIMKYLASLPLQERISRYHI
                     GKLVHIDDFPMLWSWFEDDDLLDAMGKPRLHTDAEGYFKYPYLWDLLIRRIGLEYKKL
                     LRDVDLVDTTVIIEFSRGTEHGGYRSAFQNLDPSLLEEAAILYVDVSWEESLRKNRRR
                     FNPEKPDSLLEHGIPDEKLKHMYFETDWHELTRRSPHHLEIAENKVPYAVFENDDDLT
                     SKLDRQFEERLQNRLDTLWSLYKV"
     gene            complement(717..1634)
                     /locus_tag="PLL88_02765"
     CDS             complement(717..1634)
                     /locus_tag="PLL88_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOJ00516.1"
                     /translation="MQSSRFAARLRIAYIKLNERTHGYLRIFRILWHRVGESNTAQSA
                     ASLAFYAFFSLFPLLLVTIGMISFWMANEVAFEKVVQLISQLLPFGRSLIETNLREMM
                     ALRGASGSIGFIGYLWSSSSFFSILSRNLNAALHAPARNYFQNRVIAILIVLLLIILF
                     GLSLLSSTLVNVLPEINILKINGIPIQDTKLWKNFLRIIPLLASFVFFMLLYRYVPGK
                     KIRKKSMLITSAFTSLGWALASRIFTWMLATGFVRYEVVYGTIGTILALMFWIYLICF
                     VTLVGAHLSAVLEETFNRDEILHHRRQTL"
     gene            1856..2803
                     /locus_tag="PLL88_02770"
     CDS             1856..2803
                     /locus_tag="PLL88_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00517.1"
                     /translation="MGKVRTVCGDIKESELGFTMAHEHVFMDMSGHGAPDCAWSLYDW
                     DKQLKILRDYKAIGGGCIVDAMPRKNEGRDPARLQVASIQSGVHIIACTGMFMEKRES
                     TGEYTVQDLEKMSPQEVTDFFVREITEGMDGTDIKAGWIKGGGSYCYISELQEKTLRA
                     GVRAAMTTGVSVHCHTTNGTEGLELIEIAESEGMDLTRFGIAHIDRNLDYWLHKKICQ
                     KGCYLIYDGPGKIKYYPDSMRVDVLRKLVDDGFDKQIMLSMDMGKKSHHTVYGGGPGW
                     GYVKLKFLPRLLEEGFSQETVDNFMIHNPARFYAMKNPE"
     gene            complement(2934..4457)
                     /gene="phnE"
                     /locus_tag="PLL88_02775"
     CDS             complement(2934..4457)
                     /gene="phnE"
                     /locus_tag="PLL88_02775"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOJ00518.1"
                     /translation="MNKQNLKAKEPQKPSFVPPFISSLLSMVIPGLGQILARAYSRGF
                     IILASFFSIGGLLAWRIYQKGRRYKEIQTMVEKAFDLSPILLVITILFVLLYIWIIID
                     AYFAAKKSNRGSRSYGSGLMFFLLAVFFLLGWQIGDINIGEFFSQAGTALPTIGRLMW
                     PWEKAIDRPEEIQIISARVLSPCQEQQIPQNEALEEEPYIIVSPTCGSPTEIDGTSGT
                     EFAIVGGNFNTDQEIEIWWKDPNGNEFRQRQAGEYVKVTPDDEGNFQIAIIMPYRIIS
                     STIEEKNLIWEVQARQLLSVGEAQLSAEFTLAVEKMIETIIIGMMATLFGVILSIPIS
                     FLAARNLMSENFFTKTVYYFVRTILNIVRSIEPLIWAIIATIVVGLGPFAGIIALTVH
                     SIAALAKMYSEAIESIDQGPIEAIQATGANWTQTIVYAVIPQIIPPFVSFTIYRWDIN
                     IRMSTVIGFVGGGGIGFLLQQWIRLLDYRAAGIAVWFIAITVMILDYVSAEIREKYK"
     gene            complement(4472..5260)
                     /gene="phnC"
                     /locus_tag="PLL88_02780"
     CDS             complement(4472..5260)
                     /gene="phnC"
                     /locus_tag="PLL88_02780"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HOJ00519.1"
                     /translation="MLEIKNLTKIYDDGTVALKNVSFVVNEGEFLVIIGLSGSGKSTL
                     LRCINRLIEPTEGKIIWNGKDITHIKGEELRCVRREIGMVFQHFNLVHRSSVLKNVMA
                     GRLGYTKAFPSILSKFSEADRKKAMQVIDRLEIEDQIHKRADELSGGQQQRVGIARAL
                     MQDPKMILADEPVASLDPVLAHSILSNLEKLNQEDKMTVICSLHYLDLVQRYASRVIG
                     LREGTIVYEGTKSDIRKMTDAQFKEIYGQEAERVGGDLEGTPAV"
     gene            complement(5384..6304)
                     /locus_tag="PLL88_02785"
     CDS             complement(5384..6304)
                     /locus_tag="PLL88_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOJ00520.1"
                     /translation="MSKNRLFAFGAILIIAALALTACKTEVDPWENIGTEENPIIFVA
                     VPSGETERVLASFETVADLVYADTGLVIEPFVATSYAAAIEAMCAEVPQAHMGALATF
                     SYILAHEKGCADAALVSTRFGAAFYTGQFIVRSDSGIETLADLAGKTYCRVDETSTSG
                     WIIPSIQLKAAGIDPETDLAGIVDAGSHDAVAAAVYNGDCDFGTTYVDARSTVEETYP
                     DIMDATKVIQITAEIPNDGFQFHPDFPADIRATLIAEFLHLFETEEGAAAMDEAYQYT
                     GMEEHGDEFYDAFRQILDAAGMDYAELSGG"
     gene            6548..7210
                     /locus_tag="PLL88_02790"
     CDS             6548..7210
                     /locus_tag="PLL88_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOJ00521.1"
                     /translation="MEKIILQATKRDVIGKKVKNLRLAGKLPAVMYGHNFEPTPIVLD
                     THKTVLTLKKISESTIVNIDLDGKEYAALIRDRHIDFLKNELLHLDFQVVSLTEKIRT
                     DVHIELSGEAPAVSVFNAMIVNGITEVEVEALPRDLPERITVDISGLDEIGKAVYVKD
                     IPAIDKVTILTDPEELIAIASAVKEEIIEEPVVEEAEGEAAPLTEPEVIEHGKREEEV
                     EE"
     gene            complement(7251..7829)
                     /locus_tag="PLL88_02795"
     CDS             complement(7251..7829)
                     /locus_tag="PLL88_02795"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOJ00522.1"
                     /translation="MYKLDVEQIKNRLSQEIPEISRQQKQNAAVQILLIENSSLDMVL
                     TKRSVFVQNHKHEISFPGGAFERDDEDIYQTALRETCEEINLCKRSITFLGRLNPVIT
                     HYGLTIYPFLGSIAQFDFLQAQPNWEVEEIFTIPLEWFLAQENLEERAVRVADHTRKV
                     LFYRAFQGHIVWGITAAIIYELVCELKNKAAS"
     gene            complement(7897..8742)
                     /locus_tag="PLL88_02800"
     CDS             complement(7897..8742)
                     /locus_tag="PLL88_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HOJ00523.1"
                     /translation="MIFQYQEIAQEGDLVELTSLRKGFYILRLEKGKVLQTHRGVICH
                     DDLIGKEWGAEVMSHLEKPFYLYPVTLSGMLKNTRRNTQILYPKDIGYILITLGIGPG
                     SRVIEAGTGSGGLTQALAFYVGETGHVYSYESRPQMLDLAQKNLALLELSDRVDFKLR
                     DIITGFDETNVDALFLDVPTPQDYMHQVKKALRPGGGFGCILPTANQVIRLLSALRRN
                     DFSFIDVCEIMLRFYKPEENRFRPTDRMVAHTGFLTFARAIKERKGKKSDTTASAQES
                     NEIGG"
     gene            complement(8743..9030)
                     /locus_tag="PLL88_02805"
     CDS             complement(8743..9030)
                     /locus_tag="PLL88_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00524.1"
                     /translation="MTFPVFSFGSLISVLVASVFHLIVDGDFKKYLSYLFFSWLGFWI
                     GHYLSNRVSFSLWKLGILDIGFCVAGSIVVLLLIYWIDKGAEGPHEPKEDD"
     gene            9393..10814
                     /locus_tag="PLL88_02810"
     CDS             9393..10814
                     /locus_tag="PLL88_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOJ00525.1"
                     /translation="MTTKIIFGTDGWRGTIADTYTFENVRRCAAGFGAYQKKVAPVGS
                     WIVVGYDKRFLSEEFAKAASEVLCGAGFRVYLTQDATPTPVISYSVIDKKAVGAVNIT
                     ASHNPPIDNGFKVRDQHGGAIDPEGLKEIEALIPDDLSHTKTVPYHTAVQEGLIERFD
                     AAPNYIEHVKTLVDIEKIKDAGLKILVDAMWGNGAGWFPRLLHGGKTEVVEIHNLRNP
                     IFPEMQRPEPIRPNIDAGLKKTTEIHADVLLINDGDADRVGFGDEHGVFINQLQAYGL
                     LAYYLLEVRKERGAIVKTLSTTGMLNKLGKLYDVPVYETGVGFKYVAPKMLETDAMIG
                     GEESGGYAFKGNVPERDGILAGLYFLDMMVSLKKTPSQLLNLLFEKVGAHYYDRIDTQ
                     FEGDSAEKIQNILNADPKEIGGFKVLGLNTLDGYQFALEDGGWLLIRFSGTEPIIRVY
                     CETTVQERIPAILNDGLKIAGLK"
     gene            10815..11624
                     /locus_tag="PLL88_02815"
     CDS             10815..11624
                     /locus_tag="PLL88_02815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ00526.1"
                     /translation="MNPLFDTHCHLYDRQFEPDLDAVLECALQNSVEHLLIPSENLAT
                     SLQVVNLCAIHKNIALFAAGGIHPHYAEQGKKEFSDFKTFFKDHDFVAVGEIGLDYYR
                     MLSDKKSQVEMFRACLDFAAEVEKPLLLHNREATEDMLSILQEWLRQLPGGSPLKKNP
                     GVFHSYNGDPLILDFALSHHFYLGIGGMITFKNANSLREIVKNIPVDRLLLETDSPSL
                     SPHPYRGKRNEPAHVRFVAEEISQIVELPLEKIIEKTTRNALHLLGKSTVK"
     gene            complement(11629..11710)
                     /locus_tag="PLL88_02820"
     tRNA            complement(11629..11710)
                     /locus_tag="PLL88_02820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11674..11676),aa:Leu,seq:gag)
     gene            11776..12774
                     /gene="plsX"
                     /locus_tag="PLL88_02825"
     CDS             11776..12774
                     /gene="plsX"
                     /locus_tag="PLL88_02825"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOJ00527.1"
                     /translation="MAIILDAMGSDDFPHPEIEAAVRFCNQKKDTLILVGNEDIIRPV
                     LNGYEFARDYLEIVHAPETVEMWDKPVESARAKPNNSMAMGISQLKIHKHSAFVTAGN
                     TGGAMFNAIRTLGRIQNVERPALPAVIPTQSGKCVMVDIGVNVDCKPEFLYQFAIFGS
                     TYAKIMLRIKKPRVGLLSNGEEEGKGNALVKETHQLLKNSKLNFIGNVEGKEVFGGKV
                     DVVVTDGFIGNVFLKASEAVAKFMFDNLKAELYASTLTKVGAALAKPAFRNLKKLMDP
                     DEIGALPLLGVDGLVLIGHGRSNSRAIESALGQAQLCLQANLLKAMKTALQKELGH"
     gene            12785..13483
                     /locus_tag="PLL88_02830"
     CDS             12785..13483
                     /locus_tag="PLL88_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00528.1"
                     /translation="MKVVRNDALIKRNATISQYVLFAALATLVIGIYFTITKNSADEI
                     GWAYLFLIPAYILVQVSISMGNRWSRSPRPDEVVSQSLKGLDNDFTLYIHTTAVPHLL
                     VGPAGVWIIKTYYQSGRIYLDEKKKILKQKGGGNFFTKLFASEGIGDVQHDSEILLKR
                     LDTYFQKREIPNIQDVKMVNVFSHPDVKIEAKDYAEAITTSEKLKDFMRRAAKQVNMP
                     LAEIEQITAKLPNK"
     gene            complement(13499..14218)
                     /locus_tag="PLL88_02835"
     CDS             complement(13499..14218)
                     /locus_tag="PLL88_02835"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOJ00529.1"
                     /translation="MEERLQKILSRYGYGSRREAEELIKEGKVSINGKIAVLGQKADV
                     ESDSIKVGSRLLKAKDIKKIYIAFNKPRGVLSDIVQTRDEKIVRDYIDVPEHIFIVGR
                     LDKDSEGLMVLTNDGELANVLTHPRYGHEKEYEVLLNRSPDEKQLNAWRNGVVLEDGA
                     RTGKAKVAIMRTDSRGCWVRVIMKEGKKRQIRETSARIGLFTKRIIRVRIGTLQLGNL
                     ERGKWRYLTEKEIKDLRKGLA"
     gene            complement(14236..14808)
                     /gene="scpB"
                     /locus_tag="PLL88_02840"
     CDS             complement(14236..14808)
                     /gene="scpB"
                     /locus_tag="PLL88_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOJ00530.1"
                     /translation="MGQDDMDDSALSKRRMLEAILFVSSGSVPLAEIAKLIDATPAEI
                     TALTDEMNAEYKASHGIRIMIHNNRAQLTTAPELAEKIEEFLGLEITTTLSRAALESL
                     AIIAYKQPITRPQIDEIRGVNSDGVVRTLLSKGLIEEVGRAETMGKPILYGTTEDFLQ
                     YFGLVSLVELPEISSLSAGGGNAAHTILKQ"
     gene            14875..15825
                     /locus_tag="PLL88_02845"
     CDS             14875..15825
                     /locus_tag="PLL88_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ00531.1"
                     /translation="MKILITGAAGFIGSHLCDRLIQEGHEIIGMDNFITGSPDNLTHL
                     TGSPKFSFILHDVANYIIVPDKVDAVLHFASPASPNPESPYGFPNLPIQTMKAGALGT
                     LNTLGVARANNARYILASTSEIYGDPVEHPQKETYWGNVDPIGERSVYDEAKRFAEAL
                     TMAYHRFHKINTGIARIFNTYGPRMRLDDGRAVPNFLMQALKKEPLTIYGDGEQTRSF
                     CYVDDLVDGIVKLLYSQEHEPINIGNPEEISIKDLATTINGLFNNPSGAVLVQNKAVG
                     KDPDRRQPDITRAKNLLGWSPTIDLISGLKKTIPYFQQRL"
     gene            15845..16288
                     /locus_tag="PLL88_02850"
     CDS             15845..16288
                     /locus_tag="PLL88_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOJ00532.1"
                     /translation="MNNQPKRFIKFALVGISGTIVDFAIFNFCLSILHFDSVVASVCS
                     FSIAVFNNFIWNRLWTYPESKAKPLGSQLIKFGIVSVLGLIIRTPLFAFIEAPLIQFA
                     QSRLGSNTAIPPVTLGHNLALGIAIIVVLFWNYFANRLWTYKGIQ"
     gene            16303..16887
                     /gene="rsmD"
                     /locus_tag="PLL88_02855"
     CDS             16303..16887
                     /gene="rsmD"
                     /locus_tag="PLL88_02855"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOJ00533.1"
                     /translation="MSSPRIIAGSAKGLHLKSVPGNSTRPITDRVKEALFSIIGADIQ
                     ESNFLDLFAGTGSVGIEALSRGASSATFIEKNRLAHQVLVQNIEFCKVSTSAHVIFGD
                     AFSFLQRPAHAQRSFEYVYIAPPQYKGFWEKSLRLVDDHEALLSEDGWVVAQIHPAEE
                     KELILDNFDLFDSRAYGSTLLLFYRKKSVNAGDG"
     gene            complement(16864..17361)
                     /locus_tag="PLL88_02860"
     CDS             complement(16864..17361)
                     /locus_tag="PLL88_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ00534.1"
                     /translation="MDDSFYKRLNTYTVIPRVLAFIEKDDEVLMIERSKKDAFAYKKL
                     NGIGGHIERGEDPLTAIRREVNEESGLAIHDFDLRAVIFIDIEQEKGICVFVFSSMYA
                     GGKLHPSSEGKLLWIKKDDLPNHPIIKDIPLILDLIEESKRDGKVKYVQHTYVAEELT
                     ITRVD"
     gene            complement(17406..18572)
                     /locus_tag="PLL88_02865"
     CDS             complement(17406..18572)
                     /locus_tag="PLL88_02865"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOJ00535.1"
                     /translation="MAKTIGILTGGGDVPGLNPAVKMVVLNAIEEGYKVYGIRRGWMG
                     LLHYDLDEPATHEYYVNELDDICVRKIDRNGGTFLHTSRTNPQSVKEKDIPDFLKGSP
                     EGKDLGNKTYDFTPHILKILDHLNIDVLVTIGGDDTLSYSARLTTEGFPTVGIPKTMD
                     NDVYGTDYCIGFSTAITRSVEMITNMRTSLGSHERIGVIELFGRNSGETSLISAYLSH
                     VDRAIISEVPFDMGKLAGLMVEDKQRSPSNYSIMTVSEGATFKDGEIIESGETDAYGH
                     RKLGGIGEVISEEIKRITGQNTMYQKLGYLMRSGPPDSLDRMVSMCFGTLAFQLIKNN
                     DMGKMVALRDGKYTTFPLDQVTAARRVVDVEAYYDTENYKPRVKNVLGVPMFLT"
     gene            complement(18635..19876)
                     /locus_tag="PLL88_02870"
     CDS             complement(18635..19876)
                     /locus_tag="PLL88_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00536.1"
                     /translation="MNKNNIRIQDRVAQFFQKHKPSSSIAIVVITVVGIFVLCLIVAH
                     NVIGGSDFYIKWSSLKLLRSEQINPYSSQAIQRIAETAEESLFFPLPEGASFTVPVYS
                     LFLYYPFTYLTNFETARSLWMTCLCILLALAFERKFFFKTKREVFLTIFILVSVFSLI
                     SILGGDLLALSFALLLFAYQKIKEGKFELAGMLCGLATMSPGLALAVMIFFLVMSLRQ
                     RQAGFLIWFLITTALLGFSGNLLQEQWGLQFVQANIRMIRSSFADLGLGLENIVTDIK
                     IVAPFAVIVFEWIRTHKDLDHPDKRDWLFHIILALLAVGFGERTPSLNILFLPALLQI
                     FHEWVNRDNKTAKIIGYINLGIYMAFSILILVLDAEILVGKNELPKMLFWFTGFHVIV
                     SMYWIRGWLYQDTMRNYIKPE"
     gene            complement(19960..21645)
                     /locus_tag="PLL88_02875"
     CDS             complement(19960..21645)
                     /locus_tag="PLL88_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ00537.1"
                     /translation="MKNFSCVLFDHYQHQKQKAILYFQSTDKEDITITYQDLLENAYR
                     YTREYEKTGIKPGDIIILVLQHSVDLIYAYYGAILAGAVPSIMPFLTEKLLPEKYQHD
                     FQSLIQIIQPKAIMTYRAFKQEVFDQFSERLSVDHIVLCEDVPSQTKPLTDESLPGLA
                     RDEDDVVLLQHSSGTTGLQKGVALSHKAVFQQLDHYSHAIDLDPQTDVIVSWLPLYHD
                     MGLIACFLMPILCGVPLVMMSPFDWVKAPYRLFTAISTYKGTLCWLPNFSYNFCAKKV
                     RDAQLEEVNLTTLRAVINCSEPVRYESHQAFLQKYQSYGLKEKALAASYAMAENVFAV
                     TQGGIKSTLVEDRIDRDSLQIDRKAIPVREGGSEIVMVSTGRPIEGVSVKVIDDDGKT
                     LNEREIGELIIKSDCLFSGYYNRADLTDKSFINGWFRTGDYGYIAGGEVFVTGRKKDL
                     IIVGGKNIYPQDLEAIAMEVEGVHPGRVVAFGIFNETAGTEDVVIVAEKDSTVVDKEN
                     IANTIKEKVTRNSAIALRHVLVVDEKWLIKTSSGKIARSANKDKYLQLFHSVH"
     gene            complement(21648..21896)
                     /locus_tag="PLL88_02880"
     CDS             complement(21648..21896)
                     /locus_tag="PLL88_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOJ00538.1"
                     /translation="MEKTSIIKQIGEKIASEILKERDRVIEADEALISSGLVDSFSLV
                     DLALIVEDLYGVHIDDAELSADTFDTLDQLAELVLQRL"
     gene            complement(21909..22721)
                     /locus_tag="PLL88_02885"
     CDS             complement(21909..22721)
                     /locus_tag="PLL88_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00539.1"
                     /translation="MTPLFTETIAATPTPQATETPAYTATPDTRLAPGDWMKWQVIPQ
                     VSGTVLDIYRHGQELGRNPNAFSKIGDCQNIKEAFMGFFDRQTLYLSESQQGLQETID
                     DFRGYFNRDGYAVEGGLNAPSSLNPLMADPTACQPNENPLQCEVRNNNPAFAFVSFEL
                     WYEGRSAENYEGYLRQVLDYLISEGVVPILLTKADNTEGDNSINYTIAKLAYEYDLPM
                     INWWYTAHTMSNNGMDATRSDNFHISPEAWTERSYDALETLDRIWKTVRDMH"
     gene            complement(22838..23950)
                     /locus_tag="PLL88_02890"
     CDS             complement(22838..23950)
                     /locus_tag="PLL88_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00540.1"
                     /translation="MREPKINPWRIIWKTLLVLLVVNFVYPWLKVPNFSAISLYNSVF
                     PGRQRLPFGENPGLSYNLTLDDINAMFDSHRIAAADATAEYSIVLIGDSSIWGTLLEN
                     DQTIAARLQAIVDSHLSTRDEPTRIYNLGYPTISVLKDLVIVDQALRYEPDMVIWFVT
                     LNSLVKNSYKDAPIVLNNPEKANAVINKYALDIGELPQQNYWERTFINQRRSIYDRIH
                     LQLLAPMWVSTGIDQYVPEEFSPAARDLDADDAYEGIAGDSISEGDLDISVIANFIEK
                     NPDVEVIVVNEPILISTGENSEIRYNLYYPRWAFDSYREFLSTKFAEMEIQYIDLWDA
                     VPEFLFTNSAIHYTEQGVDIVSEKIYSFIEKDLRSK"
     gene            complement(23955..25376)
                     /locus_tag="PLL88_02895"
     CDS             complement(23955..25376)
                     /locus_tag="PLL88_02895"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOJ00541.1"
                     /translation="MTIVQIACAGITSLLVRFVDRERNKEKAIFIANIFFLFLLQPSS
                     SLRSANILLPWVMLVLIFAVGMGVCEKITPKEIFQISLLSLLGFLPFLLPQLIPGLRT
                     TVLDKNVNQTILGAFLLVCVCAIGLLAIFRHKVHYLTVLLILILILFVVLKQPQIGTL
                     ASRGWRMAFRQNADLASSVDVRWIGYSYVAFRMIHVIREYQKQRLQEINLLRFINFCL
                     FFPTLTAGPIAKYDDFSNQIEEPANNFQEGMLEGGKRLFFGLFKKFVLTDSLALIALN
                     PDNARQITSSGWMWAVLFFYSLQIYFDFSAYTDIAVGLGLFLGIKLPENFNRPYLKSN
                     LTKFWDSWHITLSQWIRTYFFNPLNRTLRKSALTQSPNLILFITQVATMVIIGLWHGI
                     TWSFFFWGVWHGLGLFFQNRWSSFIKNRFNGIRENKVLNPLVGGLSTMATFTYVSLGW
                     VWFLSPSLPDAWEIFMKLFGKGF"
     gene            complement(25504..25890)
                     /locus_tag="PLL88_02900"
     CDS             complement(25504..25890)
                     /locus_tag="PLL88_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00542.1"
                     /translation="MKKTIALCASILVLAFLLVSCNSESDPADTVLNYLNALVSQDSD
                     AAVALSCAEWEEQAKLETDSFLSVKAALNAVQCQTLASDNSEAEVACSGTIDLTYNEE
                     IRSIDLSVRTYALQYQAGEWLVCGYK"
     gene            complement(25983..27113)
                     /locus_tag="PLL88_02905"
     CDS             complement(25983..27113)
                     /locus_tag="PLL88_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00543.1"
                     /translation="MITIRKVDTKSRKDVNEFVDLPFRLFKGVSQWVPPLKSGVKDVL
                     NRDKHPYHEHSDADFFLAMDGDEVVGRIAVLENKVYNKYHNKKQASFYLYDSVDDQAV
                     ADKLFECAFDWSHKRGLEYLLGPKGFSPFDGYGFQIEGSEHRQMMNMMNYNKENYPRF
                     MENLGFTKVVDWISCYAEIAKFNMPEKVQTVANKVREQGKFKVHQFKTKSELNKFVSR
                     IGDAYNKTFVNNWEYYPLTQKEVDWAFKDLTFVALPDLIKIITYKDEIVGFLLAFPDI
                     SAAFQRHPRGVLTPALILDIQREVKQSKWIEFNGVGVLPEYQGFGGNALLFDEIEKTA
                     KSHHFVHGELTNIAETAVQMRKDLLNLGVKPYKNHRIFGKNI"
     gene            27217..28269
                     /locus_tag="PLL88_02910"
     CDS             27217..28269
                     /locus_tag="PLL88_02910"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ00544.1"
                     /translation="METCNNNDCLYHEIIQQPDLAQELVQQELPRIREIVKNVRGQFD
                     YVLIAARGSSDNAARYAQYLFGINNRFQVALATPSLFTLYAAPPNLHKALVIAISQSG
                     MSPDIIEIIKNAREQQCPTICITNNVESPLSAASAFTIPIHAGEEKAIAATKSYTNSL
                     VTLAMLSAVFQENDVFTKQLLQIPEFMKTTIDSAEASLASIQRYRYIENCAVLGRGLN
                     YSTVFEIALKVKELTGINANPYSSADFRHGPIATVYKGFPVISIAPYGHAFDDMAQMF
                     YDVKLLQAETISISADDRILAESNIGFRIPKEIPETLSPIVSVIPGQFLGRQLAIERG
                     LNPDKPQGLRKVTETF"
     gene            28287..29561
                     /gene="murA"
                     /locus_tag="PLL88_02915"
     CDS             28287..29561
                     /gene="murA"
                     /locus_tag="PLL88_02915"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOJ00545.1"
                     /translation="MDKFIIEGGVPLKGEVTPSGNKNAALPILAACVLTEKPVILHNV
                     PDILDVHSMRALLESLGVKFETIDAHTWKVQAADICPAELDPELCKAIRASILLAGPM
                     LARAGYITLPPPGGDVIGRRRVDTHILALQKLGAEVECNRIFAFRCDHLKGADILLDE
                     ASVTATENAIMASVLAEGKTIIRNAASEPHIQELCQFLNMLGAKIDNIGSNTLEIEGV
                     KSLSGGEFTIGPDYLEVVSFIGAAAVTKGAITIKNAGTQYLSMVKLVFNRLGVDWKDE
                     GQDIFVPSEQCLCIEPDLNNAIPVISVMPWPAFPTDLMSIAIVIATQSKGTILFHDWM
                     YPSRMFFTDKLASMGAQIVLCDPHRCIVQGPTPLFSDDLSGPDIRAGMALLLAALSAS
                     GTSTIRNIRQIDRGYERIDEKLSLLGASIKRC"
     gene            complement(29612..29962)
                     /gene="trxA"
                     /locus_tag="PLL88_02920"
     CDS             complement(29612..29962)
                     /gene="trxA"
                     /locus_tag="PLL88_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOJ00546.1"
                     /translation="MNEPIHVTDSAFKKSVLESTLPVIVDFWAPWCGPCKMVAPILDK
                     IAKDYDSKLIVAKVNTDENQEWASRYGVQGIPTMLFIAGGKVIHRQVGSVPEGMLREA
                     VDQFLTVIQETEGK"
     gene            complement(30058..30498)
                     /locus_tag="PLL88_02925"
     CDS             complement(30058..30498)
                     /locus_tag="PLL88_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ00547.1"
                     /translation="MTKIMKSDPFYDIVSVRDTMDRMLDSYFGRSIAGFEGYGVVDLD
                     LYQTENDVVIEASIPGISPEDISISVAGEVLTIKGEVKQERENKEADYHIKERRYGSF
                     SRSVTLPTQIVADKANAEFKNGILKLTLPKAEEVKPKTITVKAK"
     gene            complement(30585..31301)
                     /locus_tag="PLL88_02930"
     CDS             complement(30585..31301)
                     /locus_tag="PLL88_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ00548.1"
                     /translation="MSSTVSSNTKILVIEDEERMARFVRLNLEQDGFQVNEALTGREG
                     LDKLRTFMPDLILLDIMLPDLDGFELLKMIREIDTVPVIMLTAKGEEEDKVRGLELGA
                     DDYITKPFSPRELVSRVKAVLRRIESASVSTDELIEVDDRLKIDFNRREIWVEGKQVK
                     LRPTEYRLLYHLVQNAGWVLTYDQILSRVWGYEYRDEPHYVRLYINYLRQKLEKDPSD
                     PKYILTERGVGYRFYDYRRK"
     gene            31449..32147
                     /locus_tag="PLL88_02935"
     CDS             31449..32147
                     /locus_tag="PLL88_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOJ00549.1"
                     /translation="MKLANLFELQSIDSKLDAYQTELAAIQAQIGRDSSVQNANQTVL
                     SMQSEVELNQRELNLLADEVERQKIKLAQSDAMLYSGKVQNPKELQDLQHEIQYLKNK
                     IADLEDTQLNQMIKLEEIQKSHQAAKTSLNTIASQFATNQSQLNARKEAIQLDFEKLH
                     RKREVVLSSIEPELIARYENLRKRKNGLAIAALENDACSACGTQLTPSERQEVRLTSK
                     LFFCPTCGRILYHN"
     gene            complement(32153..32425)
                     /locus_tag="PLL88_02940"
     CDS             complement(32153..32425)
                     /locus_tag="PLL88_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HOJ00550.1"
                     /translation="MKNLSLTVKVIPNAPKTEIVCLMDDGVLKIRVRGAPEKGKANQE
                     LIDFLADRFHVQKVDIRLISGTTSRLKKVIIAGRSDDDLQRLINPS"
     gene            complement(32422..33210)
                     /gene="pgeF"
                     /locus_tag="PLL88_02945"
     CDS             complement(32422..33210)
                     /gene="pgeF"
                     /locus_tag="PLL88_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOJ00551.1"
                     /translation="MMGFQEKNGLRYFTLPSFEENGIAHGFFTRLGGISPKPWESLNV
                     ATSVGDSRAHVVENRNRIMSIFEKNFDDVFDTWQIHSDIVHCSESPRSIEKSHEKGDA
                     VITSNPAVALMMVFADCVPVLFYDTKKNVCAIAHAGWQGTVKHIVCRTVEKMQAEYGC
                     ASEDILAGIGPSIGVDHYEVGDSTEKFIEESFPGESEKVLQRRTGKVYFDMWQANVLL
                     LERYGLKSIEVAGICTACDLQNWYSHRAEKGNTGRFAAVINVKK"
     gene            complement(33207..33860)
                     /locus_tag="PLL88_02950"
     CDS             complement(33207..33860)
                     /locus_tag="PLL88_02950"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOJ00552.1"
                     /translation="MDNFWCWFYRRYILVFCVLLLAIILGAFLAPLLAHAHFDMGAQV
                     IYTLYRITCHQLAYRSWFLFGEQAYYPLRIAGVPNVEPFEKVSGIASYDLRAAQDFIG
                     NELIGYKTALCQRDIAIGFGFVISGVGFSLSRRRWKPISILLWIVLGVLPMLVDGLVQ
                     WGGGDVFGWQLRNAYESTSLMRTATGILFGLCSGMFIFPKLDVALRITRNNHNCREP"
BASE COUNT         9388 a         8186 c         7389 g         9215 t
ORIGIN      
        1 tgggtgatcg caatgtcaac gaatatcttt aaaaaactgt ttctgctggc gcgaccggca
       61 gccggcaaga gcgagatcat gaaatatctc gcctccttac ctctgcagga gcgcatttcc
      121 cgctatcaca ttggcaagtt ggtgcatatt gatgattttc ccatgttgtg gtcgtggttc
      181 gaagacgatg atctgctgga tgccatggga aaaccacgcc tgcacaccga tgcagaggga
      241 tattttaagt atccctacct gtgggacctg ttgatcaggc gcatagggtt ggaatataag
      301 aagttgctgc gagatgttga cctggttgat acgaccgtca tcatcgaatt ttccaggggc
      361 acagagcacg gcgggtatcg ttccgctttt cagaacctcg atccctccct gctggaagaa
      421 gccgctatcc tctacgtgga tgtatcctgg gaagaatcat tgcgcaagaa ccggcgccgc
      481 ttcaaccctg aaaaacccga cagtctgctg gaacatggca ttccggatga aaaactcaag
      541 cacatgtact ttgaaaccga ttggcacgaa ctcacccgac gatcgccgca ccacctggag
      601 atcgctgaga acaaggtgcc ttatgcggtt tttgaaaatg acgatgacct taccagcaag
      661 ctagaccggc agttcgaaga acgcttgcag aaccgcctgg acacgctctg gagcctctat
      721 aaggtttgac gcctatggtg aaggatctca tcgcggttga aggtttcttc cagcacagcg
      781 ctcaggtgcg ccccgaccag cgtgacgaag caaatgagat agatccaaaa cataagggcg
      841 agaattgtgc caatggtgcc atagaccact tcgtagcgga cgaacccggt tgcgagcatc
      901 caggtaaaaa tgcgcgaagc cagtgcccag cccaaggacg tgaaggcgga tgtgattagc
      961 atgctctttt ttctgatctt cttgcccggc acgtagcggt acaggagcat aaaaaacaca
     1021 aaggacgcca gcagcgggat gattcgaagg aaattcttcc aaagtttggt atcctggatg
     1081 ggaatgccat ttattttcag aatgttgatt tccggcagca cattcaccag cgtgctggat
     1141 agcagcgata agccgaaaag tatgatcaac agcagcacga tcaagatggc gataacgcgg
     1201 ttttggaaat aattgcgcgc gggggcgtgc aacgccgcgt tcaaattgcg tgacaagatc
     1261 gaaaagaacg acgagctact ccataggtac cctataaaac cgatcgaacc agatgcgccg
     1321 cgcaacgcca tcatttctct caggttggtt tcgatcaggc tcctgccaaa cggcagaagc
     1381 tgcgaaatca actgaaccac tttctcaaaa gctacctcat tcgccatcca gaaactgatc
     1441 atgccgatcg tgaccaataa caatggaaac aacgaaaaga aagcataaaa agccagagac
     1501 gcggcagact gcgcggtatt ggattcccct accctatgcc aaagaatgcg gaaaatgcgc
     1561 agatagccat gcgtgcgttc gtttaatttg atataagcta tgcgcaatct ggctgcaaac
     1621 ctggatgatt gcattttgtt catggtaaaa gtgaattgta ctcttctttt ccgtgaattt
     1681 cacgctgagc cctcaaattc ttccgcgaac acagaattgg attcgcccac ttcgtgcaag
     1741 gtttttccat atcttcgaac gtggttgttg tgtacattgg ttcaatttcc tcgtccctcg
     1801 tagaggcaaa aagattggtg atactattaa tagaaaaaat acaggtaggt tgattatggg
     1861 taaagtaaga acggtatgtg gtgatatcaa agaatctgaa ttaggtttta ccatggctca
     1921 tgaacatgtt ttcatggata tgagcgggca tggcgctcct gattgcgcct ggagtctcta
     1981 tgattgggat aaacaactga aaatattgcg agactataaa gcaatcgggg gtggttgcat
     2041 tgttgatgcc atgccgagaa aaaatgaagg cagagatcca gccaggttgc aggtcgcttc
     2101 tatacaaagt ggcgtccaca tcattgcctg tacgggcatg tttatggaaa aaagggagtc
     2161 aaccggtgaa tataccgtgc aagatttgga aaaaatgagc cctcaagaag tcactgattt
     2221 ttttgtccga gagatcacag aaggtatgga cggcaccgat atcaaggctg gttggatcaa
     2281 aggcggcggt tcatactgct acatttctga attgcaggag aaaaccttac gggctggtgt
     2341 tagagctgct atgacgaccg gcgtttcggt gcactgccac acaaccaatg gcacagaagg
     2401 tcttgaattg atcgaaatcg cggaatctga aggcatggac ctgaccagat tcggtatcgc
     2461 gcatatcgat agaaatctcg attactggtt gcacaaaaag atctgccaaa aggggtgcta
     2521 tctgatctat gacggtccag gaaaaattaa atactaccct gatagcatgc gcgtcgacgt
     2581 tttgcgcaaa ctggttgatg acggatttga caagcaaatc atgttgagca tggatatggg
     2641 taaaaaatct caccacaccg tttatggcgg tggaccaggc tggggttatg taaagctgaa
     2701 atttctaccg cgtcttctcg aagagggttt ttcccaggaa acggttgaca atttcatgat
     2761 ccacaaccca gccaggttct atgcgatgaa aaacccagaa tagttaaaac ggttgccata
     2821 caggacgctt tcgttaattg aaagcatcct cataaagaat cttcccattt gtttaaaaaa
     2881 agtgaacaga tttatggatc tgttcacttt tttccatgga ttttttttat tctttatttg
     2941 tatttttccc tgatttccgc actaacgtaa tccaggatca taacagtgat cgcaataaac
     3001 cacaccgcaa ttcctgctgc cctgtaatcg agcaagcgga tccactgctg caacagaaaa
     3061 ccgataccgc caccacccac aaaaccgata acggtggaca tacgaatgtt aatatcccag
     3121 cggtagatgg taaacgaaac gaaaggcggg atgatctgtg gaatgactgc gtacacgatc
     3181 gtttgtgtcc agttcgcgcc ggtcgcttga atcgcttcga tcggtccttg atcaatgctc
     3241 tcgatagcct ccgaatacat ctttgccagg gctgcaatag agtgaacggt aagcgcgatg
     3301 atgcctgcaa acgggccaag cccaaccacg atggttgcaa tgatcgccca gatcagcggc
     3361 tcgatggagc gcacgatatt taaaattgtc cggacgaaat aatagaccgt cttggtgaag
     3421 aaattctccg acatcaggtt tctggctgcc agaaagctga tgggaatcga taagatcacg
     3481 ccgaataaag tcgccatcat gccaatgatg atagtttcaa tcatcttctc gacggctaaa
     3541 gtaaattcag cactgagttg tgcctcccca acgcttaaca actgacgagc ttgaacttcc
     3601 cagatcaaat tcttttcttc aatagtggag gaaataatgc gataaggcat gatgatagca
     3661 atttggaaat ttccctcatc atctggcgtc actttgacat attcacccgc ctgtcgttga
     3721 cggaattcat tcccatttgg atctttccac cagatttcta tctcttgatc ggtattgaag
     3781 tttcctccca ctatggcaaa ctctgttcct gacgtcccat ctatttctgt ggggctgccg
     3841 cacgttgggg aaacgataat ataaggctct tcttccaatg cttcgttctg cggaatctgc
     3901 tgttcctgac agggtgataa taccctggct gagataattt gtatctcttc tggtcggtca
     3961 atggcttttt cccatggcca cattaatcgt ccgatggttg gaagagctgt gcctgcttgt
     4021 gaaaagaatt ctccgatgtt gatatctccg atctgccatc cgagcagaaa aaaaacggcg
     4081 agcaaaaaga acatcaatcc tgatccgtat gaacggcttc cacgattgct tttctttgct
     4141 gcaaaatatg catcgatgat gatccaaata tacaacaaaa caaacaggat ggtgatgaca
     4201 agaagaatcg gagagagatc gaacgctttc tccaccatcg tttgaatttc tttataccgc
     4261 cgtccttttt gatagatgcg ccacgcgagc agtcctccga tagaaaagaa gctagccagg
     4321 attatgaagc cacggctgta cgcgcgcgcc agaatttgcc caagtcctgg aatgaccatg
     4381 gaaagcaaag aggagataaa tggcggaacg aaacttggtt tttgcggttc tttggctttt
     4441 agattttgtt tgttcatttt tctcttgccc attacactgc aggggttcct tcgagatcac
     4501 cccccacacg ctctgcttct tgtccgtaga tctctttgaa ttgcgcatca gtcatttttc
     4561 tgatatcgct ttttgtgcct tcgtatacga tggttccctc acgcaagcca atgactcgcg
     4621 acgcataccg ttgcaccaga tcgagataat gcaaactgca aatgactgtc attttatctt
     4681 cttggtttaa tttttcaaga tttgaaagaa tggaatgtgc cagaaccgga tcaagacttg
     4741 caacaggttc atctgccagg atcatcttgg gatcctgcat gagcgccctg gctatcccta
     4801 ccctttgttg ttgaccaccg ctcaattcat ccgcccgctt atggatctga tcttcgatct
     4861 ccaagcgatc gatgacctgc atagcttttt tcctatctgc ttcagagaac ttgctcaaaa
     4921 tgcttggaaa ggctttcgta taccccagac gcccagccat cacgtttttc agcacagaag
     4981 accggtgcac caggttgaaa tgttgaaaca ccatgccaat ttcccggcgc acgcaccgta
     5041 gctcttcgcc cttaatatgg gtaatatcct ttccattcca gatgatcttt ccttcggtcg
     5101 gttcgatgag ccgattgatg cagcgcaata aagtcgattt tcctgatcca cttagtccaa
     5161 tgatcaccag aaattcccct tcatttacaa caaagctaac gttttttaac gctaccgttc
     5221 catcatcata aattttcgta agattcttga tctctagcat ttacgctttc tccaattccg
     5281 tccttgggag tctttaaaat tatatcttca aaataataaa aaagagaaag gatataaata
     5341 tttttccttt ctctttttcc ctgcattctt tctttagatc tttttagccg cctgacagct
     5401 cagcgtaatc cattcctgca gcatccagga tctggcgaaa tgcatcgtag aactcgtctc
     5461 cgtgctcttc catgccggta tactggtatg cctcatccat tgctgccgca ccttcttctg
     5521 tttcgaacaa gtgcaggaat tcagcaatta atgtcgcccg aatatctgcc gggaaatctg
     5581 gatggaactg gaatccatca ttcgggattt cggctgtgat ttgaataact ttcgtggcat
     5641 ccatgatgtc gggataggtc tcttctacag tgctgcgggc atcaacgtag gtggtgccaa
     5701 aatcacaatc cccattgtat acggcagcgg caactgcatc atgtgaacca gcgtcaacga
     5761 ttcctgccag gtcagtttca ggatcaattc cggcagcctt gagctggata gagggaatga
     5821 tccagccgct ggtgcttgtt tcatctacac ggcagtaggt ttttcctgcg agatctgcaa
     5881 gggtttcaat tccactgtcg ctgcgaacga tgaactgacc ggtatagaaa gcagcgccaa
     5941 accgtgtgga gacgagagcg gcatcggcgc agcctttttc gtgtgccagg atatatgaga
     6001 aagtagccag agcacccata tgtgcctgtg gtacttctgc gcacatggct tcaattgctg
     6061 cagcgtagct ggttgcgaca aaaggttcga taaccaaacc agtatcagca tataccagat
     6121 cagccaccgt ctcaaaactt gccaacacac gttctgtttc ccccgatggt acagccacaa
     6181 aaatgatcgg gttctcttct gttccgatgt tttcccaagg gtccacttcg gtcttacagg
     6241 cggttaatgc taatgcggcg atgatcaaga ttgcaccaaa agcgaataac ctattttttg
     6301 acatacttct ctctcctttt ttgatttttt atatttctta ctatatatca catcttcctt
     6361 tttttcaaat gattagtctt aaatattctt taacaatcct tcttttcagg aaaatatctt
     6421 gttaaggcag gttttttcgt gccttgacca ttcctgattt ttcgcataga atcatcttct
     6481 gctattttgg gtttcccagc gaaggtaact caagcaagaa atagagtgag aggcgcaaaa
     6541 ggaaaaaatg gaaaagataa ttttacaagc cacaaaacgc gatgtcatcg ggaaaaaagt
     6601 caaaaatcta cggctagcgg gaaaactgcc cgcggtgatg tatgggcata atttcgaacc
     6661 aacccccatc gttctcgata cacacaaaac cgtattaacc ttaaagaaga tcagcgaatc
     6721 gaccatcgtc aatattgacc tggatgggaa agaatatgca gccttgatca gagatcgcca
     6781 catcgatttc ctcaagaatg aactgctgca tttggatttt caggttgtct cattaaccga
     6841 aaagattcgc acggatgttc atattgaact gagcggagag gcaccagccg tatcggtttt
     6901 caatgccatg atcgtcaatg gcatcactga agttgaggtg gaggcgctgc cacgagacct
     6961 gcctgagcgc attacggttg atatctccgg acttgatgaa attggcaaag ctgtgtatgt
     7021 caaagatatt cctgccattg acaaggtaac catcttgacc gatcccgaag agttgattgc
     7081 aatcgcttca gcggtgaagg aagaaattat cgaagaacct gtggttgaag aagcggaagg
     7141 tgaagcagcg ccattgacag aacctgaagt gattgaacac ggcaagcgtg aagaagaagt
     7201 tgaagaatag cagaagaaac tgacaaaatt ctaaccaaca aagcggcttt tcatgaagcc
     7261 gctttgtttt ttaattcaca aaccagttcg tagatgatag cggctgtaat gccccacaca
     7321 atatgacctt gaaatgctcg gtagaacaat acctttctcg tgtggtctgc tacccttact
     7381 gctctttctt ctaaattttc ttgagccaga aaccattcta gaggaatggt aaatatttct
     7441 tctacttccc aattgggttg ggcttgtaaa aaatcaaatt gcgcaatact tccaagaaat
     7501 ggatagattg tcaaaccata atgggtaatg accggattca agcgccctaa aaacgtgatc
     7561 gagcgtttgc acaggttgat ctcttcacag gtttcacgca gggcagtttg ataaatatcc
     7621 tcatcatctc tttcgaacgc cccacccgga aacgaaatct cgtgtttgtg gttctgtacg
     7681 aagactgatc gctttgtcag aaccatgtcc aggcttgaat tttcgatcag taggatctga
     7741 accgctgcgt tctgtttttg ctgacgagat atttcaggaa tttcctggga taatcgattt
     7801 tttatttgct ctacatcaag tttgtacacg gtagtactct atcgtttttt caagctaccc
     7861 ttttccgcca catcaattgc aacgccatat tgcggtctac ccgccgatct cgttggactc
     7921 ttgagcggat gccgtcgtat cagatttttt ccccttcctt tctttgatcg ctctggcaaa
     7981 agtcagaaat cccgtatgcg caaccattct gtcggttggt ctgaatctat tctcttcagg
     8041 tttataaaat cgcaacatga tctcgcaaac atcaatgaaa gaaaaatcat tcctgcgcaa
     8101 tgcgctgaga agtcttatca cctgattagc ggttggcaga atgcaaccaa aacctcctcc
     8161 gggtctcaat gctttcttca cctgatgcat ataatcttgc ggagtgggta catccaaaaa
     8221 tagcgcatca acgttggttt catcaaagcc tgtgatgatg tctcttaact tgaaatctac
     8281 cctgtcgctc aattccagta aggcaagatt cttttgtgcc agatccaaca tttgtggtct
     8341 gctctcatag ctatacacat gccctgtttc acccacgtaa aaagccaatg cctgtgttaa
     8401 gccaccagag ccggttcctg cttcaatcac tctagagcca ggtcctatcc cgagggtgat
     8461 cagaatgtaa ccaatgtctt tcggatacaa gatttgtgta ttacggcggg tattttttag
     8521 cataccgctc aaagtcacag gatataagta gaaaggtttt tccaaatgac tcatcacctc
     8581 agccccccac tcttttccga tcaggtcgtc atggcagata acgccgcgat gtgtctgcaa
     8641 gactttccct ttttcaaggc gtagaatata gaagcctttg cgcaaactgg tcaattcaac
     8701 caaatcgccc tcctgggcaa tctcctggta ttgaaagatc aattaatcat cctcctttgg
     8761 ttcgtgaggt ccctcagctc ccttatcgat ccaataaatc aataacagca ctacgatact
     8821 ccctgcaacg caaaatccga tatccagaat gcctaatttc catagtgaaa atgaaacgcg
     8881 gttgctcagg taatgcccaa tccaaaaacc caaccacgaa aagaacaaat atgaaagata
     8941 ttttttaaag tccccatcca cgatgagatg aaaaactgat gcgaccaaca cagagatcaa
     9001 agaaccaaaa gaaaatacag gaaatgtcat atttcaatta taggagattt ccccaccagc
     9061 aaacccaggg ttttgcggat aaaaactgca aatcgagcgg gtgtgatatc tctaaccttt
     9121 ttcgtaatta tatgttcaag gcgcctgctt gaggggcagc atttgccatt attatgcttc
     9181 tctatttttc attgcaatag tcccggaatg ccatgttcca gcagactgtc ggtttttttc
     9241 agggttgaag cggcgccggt tcttgcgcaa gtagcatgtt tctcaagaaa agcatgatcg
     9301 gaattgtaac aagttttatc tttttacgct cttcttctgt tgatataatc aactcagttt
     9361 acaagaatct ttatgatgta tttggaggtt caatgacaac caagatcatt ttcggaacag
     9421 atggctggcg cggcacaatc gcagacacct ataccttcga aaacgtacgg cggtgtgcag
     9481 ccggttttgg tgcctatcaa aagaaggtcg cccctgtggg ctcatggatc gttgtggggt
     9541 atgataaacg ctttctctct gaagaattcg caaaagctgc atcggaagta ttatgcggag
     9601 caggttttcg tgtgtacctg acgcaggatg ctacgcccac accagtaatt tcctattccg
     9661 tgatcgataa aaaagccgtg ggagcagtga atattaccgc ctctcacaat cctcccattg
     9721 acaatggctt caaagttcgc gatcagcacg gcggcgcgat cgatccggag gggttgaaag
     9781 agatcgaagc actgatccca gatgatcttt ctcatacgaa aaccgttcct taccacaccg
     9841 cagtgcagga ggggttgatc gagcgctttg acgctgctcc aaattatatt gagcatgtaa
     9901 aaacattggt cgatattgaa aagatcaagg acgcgggctt aaagatcctt gtggatgcca
     9961 tgtggggaaa cggcgcaggc tggtttccgc gcttgctgca cggtggaaag acagaagtgg
    10021 tcgaaatcca caacctgcga aacccgatct tcccggaaat gcaacgaccc gagccaattc
    10081 gtccaaacat tgacgcgggg ctgaaaaaga ccaccgaaat tcatgccgat gtgctgctta
    10141 tcaacgatgg agatgccgat cgggtcggtt ttggagatga gcatggcgtt ttcatcaatc
    10201 aactgcaagc ttatgggctg ctggcttact atctgcttga agtccgcaaa gaacgaggag
    10261 cgattgtgaa aaccctatcc accaccggaa tgttgaacaa acttggcaag ctttacgatg
    10321 tgccagtata tgaaaccggt gtgggattta aatatgttgc tccaaaaatg ctggaaacgg
    10381 atgccatgat tggtggggaa gaatcaggcg gatacgcttt caagggaaat gtgcccgaac
    10441 gggatggcat tctggctggt ttgtactttt tggatatgat ggtcagcctg aaaaagaccc
    10501 cttcccaatt gttgaacctg ctctttgaaa aagtgggcgc acattattac gatcgcatcg
    10561 acactcaatt cgaaggagat agtgctgaga agatccaaaa tatcctcaac gccgacccaa
    10621 aagaaatagg cggtttcaaa gttcttggac tgaacacgct ggatggctat caattcgccc
    10681 ttgaggatgg cggctggctg ttgatccgct tctccggtac cgaaccgatc attcgggtgt
    10741 actgcgaaac gaccgtgcag gaacggattc ccgcaattct caatgatggg ttgaaaattg
    10801 caggtttgaa ataattgaat cccctctttg acacccattg ccacctctat gaccgtcagt
    10861 tcgaacccga cctggatgca gtgctcgaat gcgctttgca aaattcggtc gagcacctgc
    10921 tcattccatc cgagaatctg gctaccagcc ttcaagtggt taatctatgc gcaattcata
    10981 agaatattgc cctgtttgcc gcaggcggca tccatccgca ttatgcggaa caaggaaaaa
    11041 aagagttctc tgatttcaaa acatttttca aagatcatga ttttgtggca gttggagaga
    11101 ttgggctgga ttactatcgt atgctctctg acaaaaaatc tcaggtagaa atgttcagag
    11161 catgtctgga ttttgcagca gaagtcgaaa aaccgctcct tctgcacaac cgcgaagcaa
    11221 ccgaagatat gctttccatc ctgcaagaat ggctgcgcca gcttcctggc gggtcaccgt
    11281 tgaaaaagaa cccgggggta ttccattctt acaatggcga tccgttgatt ctggattttg
    11341 ctttgagcca tcacttttat ctgggaatcg ggggaatgat cacatttaaa aatgcaaata
    11401 gcctgcgtga aatcgtaaag aacatacccg tcgatcggct cttgttggag accgattccc
    11461 cctccctctc cccccatccg tatcgcggaa agcgcaatga acctgctcat gtgcgttttg
    11521 tcgcagaaga aatatcacaa atagtggagt tgccgctaga aaagattatt gagaaaacta
    11581 cgcgcaatgc actacacctg ctgggaaaaa gcacggttaa ataaaacatg cgctgggggg
    11641 gacttgaacc cccacgcctt gcggcacatg gccctcaacc atgcctgtct gccagttcca
    11701 gcaccagcgc acttgcgaat gctattataa tcgggtataa gcatgtgtca aggcagctta
    11761 gtttaggaga tgaaaatggc aatcattttg gatgcaatgg gaagtgatga ctttccacac
    11821 cctgaaattg aagcagcggt cagattttgt aatcagaaaa aggatacatt aattctggtc
    11881 ggaaatgaag acatcattcg ccccgtatta aatggctacg aattcgccag agattatcta
    11941 gagatcgtcc acgcgccaga gacagttgaa atgtgggata agccagtcga atctgcgcgc
    12001 gccaaaccca ataattccat ggcaatgggt atcagccagt tgaaaattca caagcacagc
    12061 gcatttgtga ccgccgggaa caccggtggt gcaatgttca atgccattcg aacattggga
    12121 cgtattcaaa acgttgaacg ccctgccctg ccggctgtca tcccaaccca aagtgggaaa
    12181 tgcgttatgg tagatatcgg ggtgaatgta gattgcaagc cagaatttct gtaccaattt
    12241 gcaatttttg gcagcaccta cgctaagatc atgcttagaa tcaaaaagcc ccgtgtaggg
    12301 cttctttcga atggggaaga agagggcaaa ggtaatgcgt tggtaaaaga gacccatcaa
    12361 ttattgaaga acagcaaact gaactttatt ggcaatgtag aaggcaaaga agtttttgga
    12421 ggcaaagtgg atgtggtggt aaccgatggc tttatcggaa acgttttcct gaaagccagc
    12481 gaggcagttg ccaaattcat gttcgacaat ctcaaagccg aactttatgc atccacgctc
    12541 accaaagtgg gggcagcgct tgcgaaaccc gcatttcgaa atttaaaaaa attgatggat
    12601 cccgatgaaa tcggtgcgct acccttgtta ggcgtggacg gtttggtttt aatcggtcat
    12661 ggtcgttcga acagccgtgc catcgaaagc gcattggggc aggcacagct ctgccttcag
    12721 gcaaatcttc ttaaagccat gaaaaccgcc ctgcaaaaag aattggggca ctgaaaggaa
    12781 aaatatgaaa gtcgtccgca atgatgcatt gatcaaacgc aatgccacca tcagccaata
    12841 tgtcttgttc gcagctttag ccactctggt gatcggaatt tattttacga ttacaaaaaa
    12901 ctctgccgat gaaattggct gggcatatct atttttgatc cccgcttata tattggtcca
    12961 agtctcaatt tccatgggaa atcgctggag ccgctctcct cgccccgatg aggtcgtttc
    13021 tcaatccctg aaagggttag acaacgattt tacgttgtat attcacacaa ccgcagtccc
    13081 tcatctgctg gtcggtcctg cgggagtttg gatcatcaaa acctattatc aatctggcag
    13141 aatttacctg gatgaaaaga agaaaatctt gaaacaaaaa ggtggaggaa attttttcac
    13201 gaaattattt gcctcagagg gtatcggcga tgtgcagcat gattcagaga tccttttaaa
    13261 acgacttgat acgtatttcc agaagcgcga gataccaaac atacaagacg tgaaaatggt
    13321 caatgtcttt tctcatcccg atgtaaaaat tgaggcgaag gattatgctg aggcgatcac
    13381 gacctcggaa aaattgaaag attttatgcg ccgtgcagcc aagcaggtga acatgccttt
    13441 ggctgaaatt gaacagataa ccgccaagct accaaacaaa taaaaagaaa ggacttgctc
    13501 aggcaagtcc ttttcgcagg tccttgattt ctttttcagt taaatatcgc catttccctc
    13561 tttccaaatt tcccaattga agcgtgccta ttctgacgcg gatgatacgt ttggtaaata
    13621 atccgattct ggctgaggtt tctcgaatct gtctcttttt gccttctttc atgatcaccc
    13681 gcacccagca tcctctcgaa tccgtccgca taatggctac tttagctttt ccggtcctcg
    13741 ccccatcttc gagaacgacc ccatttctcc aggcgttcaa ttgtttctca tctggacttc
    13801 tattgagaag aacttcatat tccttctcat gtccatagcg gggatgggtc aaaacgtttg
    13861 ccagttcgcc atcattggtc aataccatta acccttcact atctttatcc aatcttccca
    13921 caatgaagat atgctctggc acatcaatat aatcgcgtac gatcttttca tcgcgcgttt
    13981 gcacaatatc agagagcacg ccgcgcggtt tattgaacgc gatatagatt ttctttatat
    14041 cctttgcttt taagagtcga ctgccaactt tgatggaatc gctttcgaca tctgcctttt
    14101 gacccagaac cgcaattttt ccgttgatcg atacttttcc ctccttgatc aactcttcag
    14161 cttcacgtct ggagccgtat ccgtacctgg atagtatttt ttgtaatctc tcttccataa
    14221 caaggacaat tttccctatt gtttgagaat ggtatgcgca gcatttccgc cgcctgccga
    14281 taagcttgaa atctcaggca gttctaccag cgacaccaaa ccgaaatatt gtaagaaatc
    14341 ctcggttgtt ccgtataaaa ttggctttcc catagtttct gctctgccta cttcttcgat
    14401 cagccccttg ctcaggagcg tgcgcaccac accatcgctg ttgacccctc gaatttcatc
    14461 gatctgtggg cgggtgatgg gttgtttata cgcaatgatg gcaagtgatt ctaaggctgc
    14521 ccgactcaac gtagttgtga tctcaagccc gaggaattct tcgatctttt cagcaagctc
    14581 aggagcagtg gtcaattggg cacgattgtt atggatcata atgcgaatgc cgtgtgacgc
    14641 cttgtattct gcattcattt catcggtcaa ggctgtgatt tcagccggtg tggcgtcaat
    14701 gagcttggcg atctctgcta atggaacgga accggaagac acgaacagaa tagcttcgag
    14761 catccttctt tttgataaag cgctgtcatc catatcatcc tgtcccatgc tataatagtt
    14821 accttcgagc gccgttcatt ataactgaga atttcgttga aagaagagct ctttatgaaa
    14881 attttgatca ccggggcagc aggttttatc ggatcgcatc tttgtgaccg tttgatccaa
    14941 gaaggacatg agatcatcgg gatggataac ttcatcaccg gtagccccga taacctgacc
    15001 catctcaccg ggagtccaaa attttcattt attttgcatg atgtggcaaa ctacatcatc
    15061 gtgcctgata aggtagatgc cgtgcttcat tttgcctcac ctgccagtcc aaacccagaa
    15121 tctccttatg gttttcccaa cctccccatc cagaccatga aagccggagc tttgggaaca
    15181 ttaaacacgc tgggtgtcgc gcgcgccaac aatgcacgct acattctagc ctccaccagc
    15241 gaaatctacg gtgaccccgt ggaacaccca caaaaagaaa cttattgggg aaacgttgat
    15301 ccgattggag aacgttcggt atatgatgag gctaaacgtt ttgcagaggc gcttaccatg
    15361 gcataccatc gttttcacaa gatcaatacc ggcattgcgc gcatcttcaa cacatacggt
    15421 cctcgcatgc ggttggatga tgggcgggca gtcccgaatt ttcttatgca agctctaaaa
    15481 aaggagccgc taaccattta cggagatggc gaacaaaccc gtagtttttg ctatgtggac
    15541 gatcttgttg acggtattgt aaaacttcta tactctcagg agcatgaacc gatcaacatt
    15601 ggcaaccctg aggaaatttc tatcaaagat ctggctacca cgatcaatgg tttattcaat
    15661 aaccccagcg gggcggttct tgtacagaac aaagcggtgg gaaaagaccc agaccgtcgc
    15721 caaccggata tcacgcgtgc caagaacttg ttgggctggt cgcctaccat cgacctgata
    15781 tccggattga agaagacaat cccatatttt cagcaacgcc tgtgattgtg aggaaagaac
    15841 tgtggtgaac aaccaaccaa aacggtttat taaatttgcc ctggtgggca tcagcggcac
    15901 gattgtcgat ttcgctattt tcaatttttg tttatcgatt ctgcattttg actcggtcgt
    15961 agccagcgtg tgctcatttt cgattgctgt cttcaacaat tttatatgga atcgcctatg
    16021 gacttaccct gagtcaaagg cgaaaccgct aggttcacaa ttgataaagt tcggtatcgt
    16081 cagtgtgcta ggcttgatca ttcgcacacc tctttttgcg tttatcgaag cccccttgat
    16141 ccaatttgca caatccaggt taggatcaaa cacagcgatt ccaccggtca ccctggggca
    16201 caatctggct ttgggcatag cgatcatcgt cgttcttttt tggaattatt ttgccaatcg
    16261 tctttggacc tataaaggaa ttcaataatc aggatgtaga atatgtcctc ccccaggatt
    16321 atcgccggat ctgcaaaagg attgcacctg aaatctgttc ctggcaattc gacacgacca
    16381 atcacggatc gcgtaaaaga agcgttattc agtatcatcg gagctgacat ccaggaatcg
    16441 aattttcttg acctgtttgc cggcaccggc agcgttggga ttgaagcgct cagccgtggg
    16501 gcatcctcag ctaccttcat cgaaaagaac cgtctcgcgc atcaagtctt ggtgcagaac
    16561 atcgaatttt gcaaggtttc taccagtgcc catgtgatct ttggagacgc tttcagcttt
    16621 ctacagcgtc ctgcgcatgc gcaaagaagt tttgaatacg tgtatattgc ccctccgcag
    16681 tataaaggat tttgggaaaa atcattgcgc ctggtcgacg accacgaagc tttattgagc
    16741 gaagatggct gggtggtggc acaaatacac ccggctgaag aaaaagaatt gatcctggat
    16801 aattttgacc tctttgattc acgcgcctac ggaagcacgc ttcttttatt ctatagaaag
    16861 aaatcagtca acgcgggtga tggttaattc ttcagccaca tacgtatgct gcacatattt
    16921 cactttgcca tcccgcttcg actcttcaat cagatcaagg atcaagggaa tgtctttgat
    16981 gatagggtga ttgggcagat catccttttt tatccacagc aattttcctt cagatgatgg
    17041 atgcagcttt cccccagcat acatggaaga aaacacaaaa acacagatgc ccttttcctg
    17101 ttcgatatcg ataaaaatca ctgcgcgcag atcgaaatca tgaatcgcaa gtccggattc
    17161 ttcgttaact tcccgccgaa tggctgtaag cggatcctcc cccctttcaa tatgtccacc
    17221 gatgccattc aattttttat aggcaaaagc atcctttttg gagcgttcga tcattaacac
    17281 ttcatcatct ttttcgatga aagcaagcac acggggaatt acggtatagg tattcagtct
    17341 tttataaaat gaatcatcca tcgcttaaat aaggatgaat tttcatccat ccctcgtcgt
    17401 catttctagg taaggaacat gggaacaccg agcacattct tgacacgcgg tttgtaattt
    17461 tctgtatcgt aatatgcttc cacatccacc acgcgtcttg cagcggttac ctggtcaagg
    17521 gggaatgtcg tatactttcc gtcccgcaac gccaccattt ttcccatatc attgttctta
    17581 attaactgaa atgccaacgt gccgaaacac atcgaaacca ttctatccaa cgaatcgggc
    17641 ggccctgaac gcatcagata cccaagtttt tgatacatgg tgttctgccc tgtgatcctt
    17701 ttgatctctt cggaaatgac ctccccaatt ccacccagct tcctatgacc atatgcgtct
    17761 gtctcaccac tttcgatgat ttctccatct ttgaatgtag cgccttcaga cacggtcata
    17821 atggaataat tggagggact gcgctgttta tcctcaacca tcaagcctgc cagctttccc
    17881 atgtcaaaag gaacctctga aataattgcg cgatctacat gcgataaata tgcacttatc
    17941 aggctcgttt caccagaatt gcgcccgaac aattcgatca ccccaatgcg ctcatgagaa
    18001 cctaacgacg tgcgcatatt ggtgatcatt tcaacactac gggtgatagc ggttgaaaag
    18061 ccgatgcaat aatcggtgcc ataaacatca ttatccatgg tttttggaat gccaacggtc
    18121 ggaaagcctt cggtggtcaa tcgcgctgaa tagctcagtg tatcatcacc accaatagtc
    18181 accaaaacat cgatattgag gtggtcaaga atctttaaaa tatggggggt gaaatcgtac
    18241 gttttgttgc caaggtcttt cccttcagga gaacctttca agaaatccgg aatatctttt
    18301 tctttcacag actgggggtt tgtgcgtgag gtgtgtaaaa aagtgccacc attcctgtca
    18361 atcttcctga cgcaaatatc atccaattca ttcacgtaat actcatgggt tgccggttca
    18421 tccaaatcat agtgtaataa acccatccaa ccacggcgaa taccatatac cttgtacccc
    18481 tcttcgattg cattcaaaac gaccattttc acggcagggt ttaaaccggg cacatcgcct
    18541 cccccggtga gaatcccaat tgtttttgcc atacttcctc cgatgtttca gtgcgaaatt
    18601 aaccggagca agtatagcat aaattgtaca aatgtcactc aggttttatg taattcctca
    18661 tggtatcctg gtacagccac ccccgaatcc agtacatgct tactataacg tgaaatccag
    18721 taaaccagaa caacatcttg gggagctcat tctttccgac caatatttcc gcatctagaa
    18781 caagaatcag gatactgaac gccatataaa tacccaggtt gatatacccg atgatctttg
    18841 cagttttatt atcgcgattt acccattcgt gaaatatttg caataaagcc ggcaggaaaa
    18901 ggatattcag agatggtgtg cgctccccga accctacggc tagaagtgcc aggatgatat
    18961 ggaaaagcca gtcccgctta tcaggatgat ccaggtcttt gtgagtgcgt atccactcaa
    19021 acacgatcac ggcgaagggc gctacgatct tgatatcggt cacgatgttc tctaacccca
    19081 agccaagatc tgcaaaggag ctgcggatca ttctaatgtt cgcttgtacg aactggagcc
    19141 cccattgctc ttggagcaag ttgcctgaga aacccaacaa ggcagttgtg atcaaaaacc
    19201 atatcagaaa accggcttgc ctttgacgaa ggctcatcac cagaaagaag atcattacag
    19261 ccagagccaa cccgggactc atggtcgcca atccgcacaa cattcccgcc aattcaaatt
    19321 ttccctcttt gattttttgg tacgcaaaaa gcaacaaagc aaaggacagg gcaagcagat
    19381 ctccaccaag aatcgaaatg agcgaaaaca cactgaccag aataaagatg gtcaggaata
    19441 cctcccgctt cgttttgaaa aagaattttc gctcaaaggc aagcgccagc aaaatgcaaa
    19501 gacaagtcat ccacaatgac ctggctgttt cgaaattggt taaataagtg aatggataat
    19561 aaagaaataa gctatatacc ggaacagtga aagacgcacc ttcaggcagg ggaaaaaaca
    19621 acgattcctc agccgtttca gctatccgct gaatcgcctg gctgctatac ggattgatct
    19681 gctctgatcg caacaacttc aagctactcc acttgatata gaaatctgaa cccccaatca
    19741 cattatgcgc aacgatcagg caaagaacaa atatgcccac gacggttatt acgacaatag
    19801 cgatagagct tgacggttta tgcttctgaa agaactgagc aacgcgatct tgaatccgaa
    19861 tattgttctt attcatagta ttttttgaga acggatatcc atattgtact tattaaatac
    19921 aaaactggca tttcatgagc ggtcttcttt agggtactat cagtgtactg aatggaacaa
    19981 ttgcaaatat ttgtctttgt tagctgaccg tgcgatctta ccgctgctgg tctttatcaa
    20041 ccacttttca tccacaacca acacatgtcg caaagctatc gccgagttcc tcgtgacctt
    20101 ctctttaata gtgttcgcta tattttcttt atctacaaca gtactatctt tttcggcaac
    20161 aataactacg tcctccgtgc cggctgtttc attaaagata ccaaatgcca ccaccctgcc
    20221 agggtgcact ccttcgactt ccatagcgat cgcttcgaga tcttggggat aaatgttctt
    20281 tccacctaca atgatcaggt ccttctttct tccggtgaca aacacctccc cgcctgcaat
    20341 gtaaccataa tcaccggtcc gaaaccatcc gttgataaat gatttgtccg tgagatccgc
    20401 ccgattgtaa tatcccgaaa acagacaatc gcttttgata ataagctccc cgatttccct
    20461 ttcattcagg gttttcccat catcgtcaat tactttgacc gatacacctt caattgggcg
    20521 accggttgat accatgacaa tctctgaacc gccttctctc accgggatgg cttttctatc
    20581 gatctgcaag ctatcgcgat caattcgatc ttcaacaagc gttgacttga tgccaccttg
    20641 tgtaacagca aatacgtttt cagccatcgc ataacttgcg gcaagcgcct tttcttttag
    20701 cccatacgat tgatactttt gtaaaaaggc ttgatgactt tcataacgca ccggttcaga
    20761 acaattgatc acggctcgta aggtcgtcag gttcacctct tccaattgtg catcccgtac
    20821 tttctttgca caaaagttat aagaaaagtt aggaagccag cacagcgtgc ccttataggt
    20881 actgatcgct gtaaaaagac gatagggcgc tttgacccag tcaaaaggag acatcatcac
    20941 caggggaaca ccgcagagga tcggcatgag aaaacaagcg atcaatccca tgtcatgata
    21001 gaggggcaac cagctaacaa tgacgtcagt ttgcgggtca agatcgatgg catgtgagta
    21061 atggtccaac tgttgaaaaa cagctttatg agaaagcgcc accccctttt gcaatccggt
    21121 cgttcccgaa gaatgctgca agagaaccac atcatcctca tcgcgtgcca gaccaggtaa
    21181 tgattcatct gtcagaggct ttgtttgcga tggaacatcc tcgcataaaa cgatgtgatc
    21241 cactgataga cgttcgctga actggtcgaa tacctcttgt ttgaaagcgc gatatgtcat
    21301 gatcgccttt ggttgtatga tctggatcaa tgattggaag tcgtgttggt atttttcagg
    21361 caagagtttt tcagtcagaa acggcataat agatggaaca gcaccagcca ggatggcacc
    21421 ataataggca tagatcagat ctacgctatg ctgcaacacc agaatgatga tatcgccagg
    21481 tttgatgccc gttttttcat attcacgggt ataccgatag gcgttttcca acaagtcctg
    21541 gtaagtgatc gttatatctt ctttgtctgt actttgaaaa tagaggatcg ctttttgttt
    21601 ttggtgttga tagtggtcga ataagacgca agaaaaattc ttcattttta taaacgttgc
    21661 aacacaagct ctgccagttg atccagtgtg tcaaatgtat cggctgacaa ctcggcgtca
    21721 tcgatgtgta cgccatacag gtcttcgacg atcaatgcga gatctacgag cgagaacgaa
    21781 tccaccaatc cgctgctgat gagcgcttcg tcagcttcga ttaccctatc ccgctctttc
    21841 aaaatctcgc tggcgatctt ttctccaatt tgcttaatga tgcttgtctt ttccatgttc
    21901 attcaatttt agtgcatatc ccttaccgtt ttccaaattc gatccaatgt ttctaacgca
    21961 tcatagctgc gttcagtcca tgcctcaggt gagatatgga aattatcgct ccgcgttgca
    22021 tccattccat tattcgacat ggtatgggcg gtataccacc aattaatcat aggcaaatca
    22081 tattcatacg ccaattttgc gatcgtgtaa ttgatcgagt tatccccttc agtgttgtca
    22141 gccttcgtca gcaagatcgg cacaacacct tcggatatca gatagtcgag aacctggcgc
    22201 agataccctt cataattctc agcagagcgc ccttcatacc acaactcaaa gctgacgaaa
    22261 gcaaaagccg gattattatt gcgcacttcg cactgcaaag gattttcatt cggctggcat
    22321 gcagttggat cagccatcaa aggattcaaa ctggatggtg catttagccc tccctcaact
    22381 gcatatccat ctcgattgaa gtatcccctg aagtcatcaa tggtttcctg cagcccctgc
    22441 tggctttccg aaagatacag ggtctgtcgg tcaaagaagc ccataaatgc ttctttgata
    22501 ttttggcaat cgccaatctt agaaaaggca ttggggtttc gccccaactc ttgcccatga
    22561 cgataaatgt ccaatacggt tcccgaaacc tggggaatga cctgccattt catccaatct
    22621 cctggagcca accgggtatc tggggtagcc gtgtaggcag gtgtttccgt ggcttggggg
    22681 gttggagtag cggcaattgt ttcagtaaat agtggagtca tgctcgcttg ctgcatgcct
    22741 tgaggtacgg gtgtaccctc agttgtactt gcttttgaat ctgaacaact tgccaataga
    22801 acggatccta gaatgagaaa aatcagcaat ttgcttttca tttactgcgc aggtccttct
    22861 caatgaatga atatattttt tccgacacaa tatcaacgcc ctgttcggta taatgaatgg
    22921 cgctgttggt aaacaggaac tcaggaacgg catcccataa atcgatatat tgtatctcca
    22981 tttcagcgaa ctttgttgaa aggaattccc ggtacgaatc aaaagcccag cgtggatagt
    23041 acaggttgta gcgaatttcg ctgttttcgc ctgtactaat caaaatcggt tcgtttacca
    23101 cgatcacttc aacgtccgga tttttttcaa taaaattcgc gatcaccgat atgtcaagat
    23161 caccctcaga aatggaatct ccggcaatac cttcatacgc atcatcggca tcgagatctc
    23221 tggcggcggg ggaaaattct tcggggacat actgatcgat tcccgtggat acccacatgg
    23281 gtgctaatag ttgcaggtgg atgcggtcgt aaatgctcct tcgttgattg atgaacgtcc
    23341 tctcccaata gttttgttgg ggcagctccc cgatgtcaag agcatattta ttgatcactg
    23401 cattggcctt ttctggattg ttcagaacga tcggggcatc tttgtaagag ttcttcacaa
    23461 gtgaattcag cgtcacaaac cagatcacca tatccggttc atagcgcagg gcttgatcga
    23521 cgatcacaag atctttcagc acggaaattg tcggatatcc cagattgtat attcgcgttg
    23581 gctcgtcgcg tgtagataag tgagaatcaa cgatggcttg taatcttgct gcgattgtct
    23641 gatcattttc caggagggtt ccccatatcg aggaatcacc gatcaagaca atggagtact
    23701 ccgccgttgc atccgcggca gcgatacgat gtgaatcgaa catcgcattg atgtcatcca
    23761 gagtgaggtt atacgataag ccgggatttt ccccgaatgg caaccgctgt ctgccgggaa
    23821 aaaccgaatt gtaaagagaa attgccgaga aattcggaac ttttaaccat gggtacacaa
    23881 aattaaccac cagaagaaca agaagggtct tccagatgat tctccatggg ttaatcttcg
    23941 gttcgcgcat agtatcaaaa ccctttgcca aataatttca taaagatttc ccatgcatca
    24001 ggaagagacg ggctcaagaa ccacacccat cccaggctga catacgtgaa ggttgccatt
    24061 gtcgataaac ctccaacgag cggattcaac actttgtttt cacgaatgcc gttaaaacga
    24121 ttctttataa aagaactcca ccgattctgg aaaaacaagc cgagaccatg ccacaccccc
    24181 caaaagaaaa aagaccatgt gatgccatgc catagtccga tgatgaccat tgtggcaacc
    24241 tgcgtgataa acaagatgag attgggtgat tgggttaaag cggacttgcg caaagtgcgg
    24301 ttcaacgggt tgaaaaaata cgtccgtatc cattgcgaaa gcgtgatgtg ccagctatcc
    24361 caaaatttcg tcaggttgct cttcagataa ggacggttga aattttctgg caatttgatg
    24421 ccgagaaata acccaagccc gacagcgata tcggtataag cgctgaaatc aaaatatatt
    24481 tggagggaat aaaaaaacag aaccgcccac atccagccag aagatgttat ctgccgtgca
    24541 ttatcggggt tcaaagcgat gagcgccagg ctatcagtca gaacaaattt tttgaacaat
    24601 ccgaaaaata gccgttttcc tccctccagc ataccttcct gaaaattgtt cgccggttct
    24661 tctatctggt tggaaaagtc atcatattta gcaattggtc ctgcggtcaa cgttggaaaa
    24721 aacaggcaaa aatttatgaa ccgcagcagg ttaatttctt gaagccgttg tttttgatat
    24781 tcgcggataa catggatcat gcgaaacgca acataggaat accctatcca gcgaacatcc
    24841 acgctggaag ccaggtcagc attctgccgg aatgccattc tccaaccccg gctagccaaa
    24901 gtgccgattt gaggctgttt tagaacaaca aaaaggatca ggataaggat caagagaact
    24961 gttaaataat gcactttgtg cctgaaaatg gcgagcaggc cgattgcaca aacacagacg
    25021 agaaggaaag cgcccagaat agtttggttt acatttttgt ccaatacggt tgttcttaat
    25081 cccgggatca gttgtggtag aagaaaaggc aaaaaaccca acaaagaaag caaactgatc
    25141 tggaatattt cttttggcgt gattttctca caaaccccca tcccaaccgc aaagatcaaa
    25201 accagcatca cccacggcag caagatgttg gcggaacgaa gggaggacga cggttgtaag
    25261 agaaataaaa aaaatatatt cgcaatgaat attgcttttt ctttattcct ctccctatcc
    25321 acaaatcgaa ccagcaaact ggttattcca gcacaagcaa tttgaacgat ggtcatttag
    25381 aaaccctgat agatccacag cggggaggta tcgacactaa agatcaatac agccataatg
    25441 atcagcacca aagcaaccac ccacatgttt tctgcgcgtt tatttccgtt catagacgaa
    25501 agattattta tacccacaca ccagccattc accagcctgg tattgtagag cgtaagtgcg
    25561 aacactgaga tcaatggagc gaatttcttc gttataggtg agatcaatcg ttccagagca
    25621 cgcaacctca gcctctgaat tgtcggaagc taacgtctgg cattgcacag cattgagcgc
    25681 cgctttgaca gaaaggaaag aatcggtttc cagcttcgcc tgttcctccc attctgcaca
    25741 cgataaggcg accgctgcat cagaatcttg acttacaagt gcattaagat aattcaacac
    25801 agtatctgcc ggatcagact ctgaattaca gcttaccagc aaaaaagcta gaacgagaat
    25861 tgaagcacat agcgcaattg tctttttcat gatatctcct gtgcctattt taatgcatgt
    25921 ttatggggaa tcaaaaaagg agatggtatt tgccaccatc tccttttttg ccgctcttcg
    25981 ttttagatgt tttttccaaa aatacggtga ttcttgtacg gttttacacc caaatttagc
    26041 aaatcttttc tcatttgcac agcggtttca gcgatattgg tcaattcgcc atgcacaaaa
    26101 tgatgactct tggcggtctt ctcgatctca tcgaacagca gagcattccc tccaaagcct
    26161 tgatattccg gtaatactcc cacgccattg aactcaatcc acttggactg ttttacttct
    26221 ctttggatat ccaagatcaa tgccggggtt aaaacaccgc ggggatggcg ctggaaagct
    26281 gctgaaatat ccggaaaggc tagtaggaag cctacaattt catctttgta tgtaatgatc
    26341 ttgatcagat cggggagcgc tacaaatgtc agatctttaa aagcccaatc cacttctttt
    26401 tgtgtcaacg ggtagtattc ccagttgttc acaaaggttt tattgtacgc atctccgatg
    26461 cgtgaaacaa atttgtttaa ttctgatttt gttttgaatt gatgaacttt gaatttcccc
    26521 tgttcccgca ccttatttgc cacagtctgc actttttcgg gcatattgaa ctttgctatt
    26581 tcggcataac aggagatcca atccacaact ttggtaaatc ccagattttc catgaatctt
    26641 ggataattct ctttgttata gttcatcatg ttcatcatct ggcgatgttc agaaccttct
    26701 atctgaaaac cgtatccatc aaatggcgaa aacccttttg gacccagcaa gtattccaac
    26761 ccccgtttat gcgaccaatc aaaggcacat tcaaacaatt tgtcagccac cgcctggtca
    26821 tctacactgt cgtacaaata gaaggatgcc tgttttttat tgtgatactt gttgtaaacc
    26881 ttgttttcga gcacggcaat tcttcccaca acctcatcgc catccattgc cagaaaaaaa
    26941 tcggcatcgg aatgctcatg gtagggatgc ttatcccgat tcagcacatc cttgacgcca
    27001 cttttcaacg gcggcaccca ctgcgacaca cccttaaaaa gacgaaacgg caggtcaacg
    27061 aattcattca catcttttct tgattttgta tctactttac ggattgtaat cataatttct
    27121 ctccatgaaa taaaaacagt atactctagc tttggaatct ggttatacta atacagtata
    27181 caaaacacca aattcggaaa gaagtgatga taaaaaatgg aaacttgcaa taacaacgac
    27241 tgcttatatc acgaaattat tcaacagccc gatctggctc aagagctggt ccagcaggaa
    27301 ttgccaagga tcagggaaat cgtgaaaaat gtgcgcggtc agtttgatta tgtgctcatt
    27361 gcagccagag ggagttccga caacgcagcc agatacgccc aatatctctt tgggatcaac
    27421 aaccgttttc aagttgcact ggcaactcca tcattgttca ccctgtatgc tgctcctccc
    27481 aacttgcata aggcgcttgt tattgctatt tcgcaatctg gcatgtcgcc agatattatt
    27541 gaaatcatca aaaacgctcg tgagcagcaa tgtcctacca tttgcatcac aaacaatgtc
    27601 gagtcaccct tatctgcagc ttccgccttc accatcccca ttcacgcagg tgaagaaaaa
    27661 gcgatcgctg ccacaaaatc gtacacaaat tctcttgtca cactcgccat gctttccgct
    27721 gtctttcagg aaaacgatgt gttcaccaaa cagttattgc agatccccga attcatgaaa
    27781 accacgattg attctgctga agcctctctt gcatccatcc aacgctatcg atatattgaa
    27841 aattgtgcgg ttttaggcag aggtttgaac tattccactg tgtttgaaat tgcgctgaag
    27901 gtaaaagagt taaccggtat caatgccaac ccctattcct ctgcggattt ccggcatgga
    27961 ccaatcgcca cggtttacaa gggatttccg gtgatcagca ttgcccctta tgggcatgcc
    28021 ttcgatgata tggcgcaaat gttttacgat gtcaaactgc tgcaagcaga aaccataagc
    28081 atatctgctg atgatagaat tcttgctgaa agcaacatcg gattcaggat cccgaaagaa
    28141 atacctgaaa cactttcccc gatcgtaagc gtaatcccgg gtcagttctt agggcgacaa
    28201 ctagccatag aaagagggtt gaaccctgat aaaccccaag gattgcggaa ggtaaccgag
    28261 actttctaac tcaatggagg tttttcatgg ataaattcat catcgagggg ggagttccct
    28321 tgaagggtga agtaacacca tctggaaata agaatgctgc gcttcctatt ctggcggcgt
    28381 gtgttttgac agaaaagcca gtcattcttc ataacgtgcc tgatattctc gatgtgcata
    28441 gcatgcgcgc tttgttggaa agcctgggag tgaaattcga aacaatcgat gcgcatacct
    28501 ggaaagttca ggctgctgat atttgtccgg ctgaactgga tcctgaactc tgtaaagcaa
    28561 tccgtgcatc aatcctgttg gcaggaccca tgttggctcg cgcggggtat atcacgctcc
    28621 ccccgcccgg cggtgatgtg atcggtagaa ggagagtgga tacccacatt ctggctcttc
    28681 agaaacttgg agctgaagtt gaatgcaatc gtatctttgc ctttcgctgc gatcatttga
    28741 aaggggcaga cattttgctg gatgaagcca gtgttaccgc caccgaaaac gcaatcatgg
    28801 ctagtgtttt ggctgaaggg aagacaatca tccgcaacgc agcttctgaa ccccatatcc
    28861 aggagttatg ccaattcctg aatatgctgg gggcaaaaat cgacaatatc ggttcgaaca
    28921 cccttgagat cgaaggggta aaatcgcttt caggcggcga attcacgatt ggacccgatt
    28981 atcttgaagt tgtcagtttt atcggggctg cggctgttac aaaaggagcg atcaccatta
    29041 agaacgccgg aacgcaatac ctctccatgg tgaaactcgt tttcaaccgc ctgggggtgg
    29101 actggaaaga tgaggggcaa gatattttcg tgccgtctga acaatgtctt tgtatcgaac
    29161 cagacctaaa caacgccatc cctgtgatca gtgtgatgcc ctggcctgcc ttcccaactg
    29221 atctaatgag cattgccatt gtgatcgcca cgcaatcaaa aggcaccatc cttttccatg
    29281 attggatgta tccaagccgt atgttcttta cagataaatt agccagcatg ggcgctcaaa
    29341 tcgtgctttg tgacccccac cgctgcatcg tgcagggacc aacccctctc tttagtgatg
    29401 atctctccgg accggacata cgggcaggca tggctctttt gctggctgcg ttatctgcgt
    29461 ccggaacgtc cacaatccgc aatatcagac agatcgatcg agggtacgaa cgaatcgatg
    29521 aaaaactttc tctcctggga gcgtcgatca aacgatgtta aagaaaaaag acccgttagg
    29581 gtcttttttg tgcgatacaa cacgttatac gttatttgcc ttcagtttct tgtattaccg
    29641 taagaaattg atccactgcc tctcgcagca tgccttcagg aaccgaaccc acctgacggt
    29701 gtatcacttt tccaccagca ataaagagca tggtcggaat tccctgaacc ccatagcgag
    29761 aagcccattc ctgattctca tcagtgttca cttttgccac tatcagtttg ctgtcataat
    29821 ctttggcgat cttgtctaaa ataggtgcta ccattttgca cggtccacac cagggagccc
    29881 agaagtctac gatcactggc aaagttgatt cgagcaccga tttcttgaag gctgaatcgg
    29941 taacgtgaat tggttcattc attatagtat ctcctaatct ttcgaaaaca aaacctttcc
    30001 gaaattgtac aggatttttc aaaatcggaa aggtttctta caaaatttat attcttctta
    30061 ctttgctttg acggtgatgg tctttggttt gacttcttcg gcttttggaa gggttaattt
    30121 caagatgcca ttcttgaatt cagcattggc tttatcggcg acgatctgtg taggaagggt
    30181 taccgagcga ctgaaactgc cgtatctgcg ttctttgata tgataatcgg cttctttgtt
    30241 ctcgcgttcc tgctttactt cgcctttgat ggtcagaacc tcgcccgcga ctgagatact
    30301 gatatcttcc gggctgatac cagggatcga agcttcgata actacatcat tttctgtctg
    30361 gtaaagatcg agatcaacaa caccataccc ttcaaaaccg gctatggatc gaccgaaata
    30421 gctatcgagc atcctatcca tcgtatcccg tacgcttaca atatcataga acggatcgct
    30481 tttcattatc tttgtcattt cgacctcctt ttcttgcaac aagaaagata caacgcttgt
    30541 attaatatca tacaagcgtt gcataggaat tttataaaat tgctttattt tctacgataa
    30601 tcataaaatc ggtatcctac cccacgttcg gttaatatat acttcggatc ggaaggatct
    30661 ttttccaatt tttgccgcaa ataattaata tacaacctta cgtagtgagg ctcatctcga
    30721 tattcatatc cccatactct tgaaaggatc tggtcgtatg taagcaccca ccccgcattt
    30781 tgtaccagat ggtaaagcag acggtattct gtagggcgta attttacctg cttcccctcc
    30841 acccaaatct cacgccggtt gaagtcaatc tttaatcggt catccacttc gatcaattcg
    30901 tcggtgctaa cgctggcaga ttcaattcgc cgtagaactg ctttcacgcg actcaccagt
    30961 tcacgagggc tgaacggttt ggtgatgtag tcatctgcac ccaattccag accgcgcacc
    31021 ttatcttctt cctcgccttt tgcggtgagc atgatcaccg gaacagtgtc gatttcgcgg
    31081 atcattttta ataattcgaa gccatcaaga tcggggagca tgatatctaa aaggatcaga
    31141 tcgggcatga aagttcgtag cttatccagt ccttcccttc ccgttaacgc ttcatttacc
    31201 tgaaaaccat cctgttccag attcaatctg acaaagcgag ccatcctctc ttcatcttca
    31261 ataacaagga tctttgtatt cgatgaaact gttgaactca tggtttatcc tcctgtactg
    31321 gttctccgtt cggtcactat tcttccatga tcgtttttca atgaatgtct ctcatgcatg
    31381 ttatcatatt gtaatatgat aacaggttca atatcagtat tatataaata tccttgaaga
    31441 agataacgat gaaactcgca aatctttttg aattacaatc gatcgactca aaattggatg
    31501 cataccaaac ggaacttgcc gccatacaag cacaaatagg tcgagattca agcgtgcaaa
    31561 atgccaatca gacggtcctt tctatgcaaa gtgaggttga attaaatcaa cgagaactga
    31621 atttgctggc tgacgaagtg gaacggcaaa agatcaagct agcccaaagt gatgcaatgc
    31681 tctatagtgg gaaagttcaa aatcccaaag agctgcaaga tttgcagcac gagatccaat
    31741 atttgaaaaa taagatcgca gacctggaag atacacagct caatcaaatg atcaaactgg
    31801 aagaaataca aaaatcgcat caagcagcga aaacgtcatt gaatactatc gcttcgcaat
    31861 ttgcgaccaa ccaatcgcaa ttgaatgcgc gcaaagaagc cattcagctc gattttgaaa
    31921 agcttcaccg aaaacgtgaa gtggtgcttt cttccattga acccgagctg attgcacgtt
    31981 atgaaaatct tcgtaaacgg aagaatggat tggcaatagc agcattggag aacgacgcat
    32041 gttctgcctg cggcacacag ttaacaccaa gcgagcgtca ggaagttcgc ttaacttcca
    32101 aactcttttt ctgtcccacc tgcgggcgta ttttgtatca caattgaaat catcaagaag
    32161 ggttgatcag tctttggaga tcatcatcac ttctgccagc aatgatgacc ttttttaatc
    32221 tcgacgttgt acccgagatc aacctgatat ccactttctg cacatgaaaa cgatcggcta
    32281 aaaaatcaat cagttcctgg ttggcttttc ctttttcggg agcccccctt acccttattt
    32341 tcaatacgcc atcatccatg aggcagacga tctcagtctt tggcgcgttt gggatcactt
    32401 ttacggtcaa ggaaagattt ttcatttttt cacgttgata accgcggcaa aacgaccggt
    32461 gttccctttt tcggcacgat gagaatacca attttgcaga tcacaggcag tacatatccc
    32521 tgcgacctcg atggatttca aaccatatct ttcaaggagc agcacattcg cctgccacat
    32581 atcaaagtac accttccccg ttcttcgttg aaggaccttt tcgctctcac ccggaaagct
    32641 ctcttctatg aacttttccg tcgaatctcc cacttcataa tgatcaaccc cgatagatgg
    32701 tccaattccg gccaagatat cttcagacgc acagccatat tccgcctgca ttttttcaac
    32761 agtccgacat acaatatgct ttacggttcc ctgccacccg gcgtgcgcaa tggcacagac
    32821 attctttttg gtgtcgtaga acagcaccgg aacacaatcg gcgaatacca tcatcaaagc
    32881 tactgccgga ttcgacgtaa taaccgcatc gcccttttca tgggattttt cgatagaacg
    32941 cgggctttct gaacaatgca caatatcgct gtggatctgc caggtatcaa aaacatcatc
    33001 aaagttcttt tcaaagatgc tcatgatgcg gttacgattt tcgacgacat gcgcgcgcga
    33061 atcgcctacg gatgtggcaa cgttcaatga ctcccaaggc ttagggctta ttcctcccaa
    33121 tcgggtaaaa aagccatgcg caatcccatt ttcttcaaaa gacggcaggg tgaaatatct
    33181 taatccattt ttttcttgga atcccatcat ggctccctac agttatggtt gttccgggtt
    33241 atccgtagag caacgtcaag ttttggaaaa atgaacatac ctgagcataa cccgaacaga
    33301 atgcccgtag ccgtgcgcat gagcgaggtg ctctcatagg catttcgcaa ctgccagccg
    33361 aaaacgtctc ccccgcccca ctgaacaagt ccatcgacca acatcggtaa gaccccgaga
    33421 actatccaca acaaaatgga aatcggcttc catcttcgtc ttgatagtga aaaacccact
    33481 cctgaaataa caaatccaaa gccaatggct atatctcgct ggcaaagtgc cgttttgtat
    33541 ccgatgagct cattgccgat aaagtcttgc gcagcgcgga gatcatagct tgcaatgcca
    33601 ctcacttttt caaagggctc aacattggga acccctgcaa tacgcagggg gtaatatgcc
    33661 tgctcaccga acaaaaacca ggaacgatat gccagttgat ggcaggttat ccgatagagt
    33721 gtgtagataa cctgtgcacc catatcgaaa tgcgcgtgcg ccaacagagg agccaggaac
    33781 gctccgagta tgatcgcgag aaggagaaca caaaaaacca ggatatatct tcgatagaac
    33841 cagcaccaga agttgtccat tgttttcgat caatacaggg gttctctaac atggatcatg
    33901 gttccgctgt aatcggagaa tgtttcatcg cccggattca aactgatata gcgatttgac
    33961 caacgaaata cccatttcgc gtcttcaggg ctggcgttca cacagccgtg ggatcgcgca
    34021 gaaccaaaat cgctgtgcca gaaggtagca tggatcgcca cgcccgtgcc gacaaaaagg
    34081 cacgtccacg gtatcgccat ggtatcccaa ccggcacctg tgcccccacc gctcatgtga
    34141 atggaaatcg cttttcacca gatccagtga tctccaac
//
LOCUS       DAOSDS010000013        34167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_641_length_34167_cov_5.189142, whole genome shotgun sequence.
ACCESSION   DAOSDS010000013 DAOSDS010000000
VERSION     DAOSDS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 34167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34167
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_641_length_34167_cov_5.189142"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLL88_02955"
     CDS             <1..1077
                     /locus_tag="PLL88_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00553.1"
                     /translation="EKIDVIEWNKDPRIYIAKALSPARVNGVYLNETDSNAKTALVVV
                     PEDQLSLAIGRDGQNARLAARLTGWRIDIKSLPEAVSDWLFALQNNEEYAEKAEQFKE
                     EIAKTEDIMARKTEGRILNMEDYDSLARFVDLMEKGLFAERQEVIAAREKAIQEALQT
                     IPKHAFSVPLSEEIIPQTILSILGDSGIQNLGDLALELKSNPDSILGLPGIGPKALEK
                     IESVVNDLSRFIPAEPEPDHEVSEAVTVADEFVEPQEAVQEASETMDLPEETTPEREA
                     EQQPEQEEGQELSFEEMFKIETQKTNGGSFEDEDDELADKSGKKKKKKKYQEIEYDPD
                     RDMTLVKKKHKRGSNDWGLDDNGN"
     gene            1093..1395
                     /locus_tag="PLL88_02960"
     CDS             1093..1395
                     /locus_tag="PLL88_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOJ00554.1"
                     /translation="MKKQKLRQKHIPQRTCVGCREVLAKKELIRIVRTSEGILVDPTG
                     KLNGRGAYIHLQKECWERALKGSLSKALRTDLSEQDRKYLEAYQMNLPESKSKMQD"
     gene            1483..3267
                     /gene="infB"
                     /locus_tag="PLL88_02965"
     CDS             1483..3267
                     /gene="infB"
                     /locus_tag="PLL88_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOJ00555.1"
                     /translation="MSENGKKTVELPASITIRDLGERIGFSPIQIIKILMSNGVMANI
                     NQIIDFDTAAIVCAELGIDATLEVAEIKEEELGEVPLWRKLIENEDPANLAKRPPVIT
                     ILGHVDHGKTTLLDAIRHTNVAGEESGGITQHIGAYQIVHNNQKITFLDTPGHAAFTA
                     MRARGAQGADIVILVVAANDGVMPQTKEAIAHAKAARVPIVVALNKIDRDDANPDMVK
                     RQLADAGLVPDDWDGDTMVVPVSAKTKKGLDDLLEAISLVADSCKILANPKGKVIGTV
                     IEAKVDKARGVVATLLVQNGTLRQGEAVVVGVAHGKIKAMYNFLGEKIEEAGPSTPIQ
                     ILGLNEVPQAGDIFQVAASDKDARAIVADRKAKLSKARQNKKQASLEDLFNRFQAGEV
                     RELRLIIKADVQGSLDPIISTVNEINQKEIKINILHAETGNISESDVMLASASNAIIV
                     GFNVEAETSANKLAEVEGISIRLYDIIYRLTEDIEKAMKGMLEPEKQEVVLGKAQILA
                     VFSFSKVGKIAGCKVTDGVIRRNANMRVVRNGEVLADNEIASLRREKENVREVREGYE
                     CGIRVKGFNDFAEGDTLICYTFEEKQAI"
     gene            3286..3678
                     /gene="rbfA"
                     /locus_tag="PLL88_02970"
     CDS             3286..3678
                     /gene="rbfA"
                     /locus_tag="PLL88_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOJ00556.1"
                     /translation="MPSITRLKRIADRMQQDLSEMLVQGNIHDPRLEGIFITDIRVDR
                     ELAYANIFVSALEGKAREEEILEGLKHARGFLRKELARRIQLRTFPELRFYWDPTPER
                     AERIEELIDTLQTEREKKEQKNDQGSSG"
     gene            3656..4612
                     /locus_tag="PLL88_02975"
     CDS             3656..4612
                     /locus_tag="PLL88_02975"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOJ00557.1"
                     /translation="MTRDPAASQRIWQKIDAAESIILVTHVRPDGDAVGSVLGFAEAL
                     RGYGKEVHIAFEETLPVRFRFLEEKEKPTSISTSGLTVTVDCADQARMPKIFAKKKID
                     INIDHHATNTQFGDLNYVDAEAAATAEIISDLLLDWNIPITSSAASALLMGIITDTIG
                     FRTPNTTPHTLRTAAKLSELGANLSEIYQRSLTDMTWEASLLWGFALNKLQKEGKIIY
                     TVITCEDRKRSGFTDRDDADLTNFLSSIQDAEIAILFNQQSEKSVKVSWRSRSKVNVA
                     DLASQFGGGGHPAAAGAEMELSLQETQQKILEATKAYLLKIE"
     gene            4627..5535
                     /gene="truB"
                     /locus_tag="PLL88_02980"
     CDS             4627..5535
                     /gene="truB"
                     /locus_tag="PLL88_02980"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOJ00558.1"
                     /translation="MDDRRTQLKNTVSGVLVVDKPIGMTSHDVVQIIRNGTRIKRAGH
                     TGTLDPRASGVLVILLGPAVRLSEYVSAEDKQYHAVIQLGKKTDTFDEEGAVTGTSPV
                     DITAEQFEEALKSFIGEIEQVPPPYSAVKVNGRKAYEFAREGEDVELEPRIIQVYNLE
                     LLEWDPPQALVDVHCSSGTYVRSLANDLGEKLGCGAMLAGLRRTKSGKFTLKDATPLR
                     KLSEAFQDGTWYKYLIPAADALGEWPTIYLTAEEVELVQHGHRIRRDEKIGDMARAIT
                     EQGELVALMQYDEESGEYQPKKVFFS"
     gene            5538..6449
                     /locus_tag="PLL88_02985"
     CDS             5538..6449
                     /locus_tag="PLL88_02985"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOJ00559.1"
                     /translation="MPTINHPSLSQITIGSFDGLHAGHQTIIHHLVAGAHSQGQKAVL
                     VTFYPNPTVFFKKIHTPFYLTTNSEKIALLQKLGIDETIILPFNQELAQSSAEDFIRY
                     LLQRVPFNTMYIGYDFHFGADRAGDRGKLFELGKKYGFTVQVQQPVDLNATPISSSMI
                     RSIISEGRVEDLSALLKRWYSMEGEVIHGDGRGKKMGIPTANIAYEPQKLLPPNGIYA
                     TRIQIGVETRPAATNVGFRPTFYETPSIKTVEAFILDWECDIYGKTVTLEFVRRLRDE
                     IQYQNTQALTAQIRKDIALTRKIIDHE"
     gene            6442..7863
                     /locus_tag="PLL88_02990"
     CDS             6442..7863
                     /locus_tag="PLL88_02990"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOJ00560.1"
                     /translation="MSEKRRVYLLDNKKLSPETIAVTFAKTSRSPQSFDQIAQELSDE
                     KSAQFHERWVVGYGHASVAEHAVLHIAIENVSRLAIECLESNRLASYTEKSSRYQTWD
                     ADCFFIPDEIKGTSLEDAYSNTINALFQKYHNAIPFLQTTIANICRATSKPISDREIH
                     ARCMDVCRYYLPAAAMANVGVTINARELEHALRKMLSHSLLEVRQIGQEIKQVAISHL
                     PTLVKYADEVAYFKQAEERTTQLIANAHPASHGKNWCTLLDHDERFEHHILAALLLRY
                     GQGDYAAQENKLHDFSKEQQQTLMDILFGRLDAHAMPLRELEYSFFLFELLMDQGAYF
                     EFKRHRMMTQTAQVLTPYYGYAIPRLISDAGLEADFHEAMAIAQEGYTQIAATHPAVA
                     SYVIPNAYNRRLLAGCNLRTVIHFTNLRTASNAHFAMRRVANRMAEEIRERMDLFKPY
                     FYADPPETWQQIETEFFTDPIYP"
     gene            7962..9305
                     /locus_tag="PLL88_02995"
     CDS             7962..9305
                     /locus_tag="PLL88_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOJ00561.1"
                     /translation="MSKIEFVIKRNGATVPFTPQRISNAIYRAAVAVGGRDKETAEKL
                     ALQVVEYLEQNFPPTKYPSVEDVQDAVEKILIENGHSKVAKAYILYRDERARMRSSRK
                     DQTIQPSQNIPWAKIWQVLDWASSRDLHTVERYNQRIRNGEASEIIRDSEIFYEEDIK
                     IAARMVLERRDQVKVVLITGPSSSGKTTTTIKLGNILQQSNIKLVTLNVDHYFFDLDD
                     HPKDEFGDYDYEAPQALDLNLISEHLATLITGKEVKIPFYDFKTGKRQLNHTPMKLKE
                     NEMILIDSLHGLYPALTEGVEENRKFKLFLEPLLQMKDDEGKYIRWTDIRLMRRMLRD
                     AAFRALNPESTLTHWHYVRTSELRNIIPNINRADSIINSAMPYELSIYKPKLLHLFEK
                     WPEKFKDDPLRQDAYERSSRILTLLQKIEPLSDDSIVPSDSVIREFIGGSSLSYH"
     gene            9309..9860
                     /locus_tag="PLL88_03000"
     CDS             9309..9860
                     /locus_tag="PLL88_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOJ00562.1"
                     /translation="MPYAVVLYLDAQASHPIRQAIQELALKDVAPYMEVHSVQPHVTL
                     AIYDNLDCQLCERKIARAARIMRGLTLNFAYLGVFHSESVVVYLGATPTQELLAIQKR
                     VHGILNSIASNPWDLYLPGKWIPHCSLAIELSPRKLQKAMQVCMEIALPLTISIVSLG
                     IMQFEPLQPIYTYTIHGYKIIKE"
     gene            complement(9863..10777)
                     /locus_tag="PLL88_03005"
     CDS             complement(9863..10777)
                     /locus_tag="PLL88_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOJ00563.1"
                     /translation="MAVERAPPIDSCIASLASVACAGGAGENPAGIVVMLKVLLFAPI
                     STSLYSRLVLHGLLREQGVQVVGVAVRSPWNMQRIRSEWKRDGSRLAQKVIRKYLLRE
                     NAFRGIAVENLASFAASVQLPKGTLKELAGSWNLPYLVCRDLSADECAQFVFQHAPDV
                     IVFTGGGLIRQNILQIPPLGVLNCHTGILPAYRGMDVVEWTALECAIQKVGFGVSLHY
                     MDKGVDTGPVLLVKRFDPVEGDTFSTIRMRLEVEMVRVMLAGVRGIVSGDLHAHAQTP
                     LAGRQYFVMHPRLKAVAEENLKKSLRNS"
     gene            complement(10669..11349)
                     /locus_tag="PLL88_03010"
     CDS             complement(10669..11349)
                     /locus_tag="PLL88_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00564.1"
                     /translation="MASIQKFLIALDNSLAERFLQPDRIDAFIERESSKVFQRVQRSG
                     LECFPEIEREQVRRLCQTLPSLGNKMEASGFHVADEAQTDAREKDDRRGTLENERVLT
                     KGVLQYPGAEVLLDMLESGERGSSLLETNMEVLLRHVEVKRQQALRRYSEPLTRTLRW
                     RERCAYAALFCRYADLKKDGRFMNAALKLSEWLWKERRRSIPALPALPLLLALVEQER
                     ILQGLWSC"
     gene            complement(11357..12403)
                     /locus_tag="PLL88_03015"
     CDS             complement(11357..12403)
                     /locus_tag="PLL88_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00565.1"
                     /translation="MKLTIVEQYPRMTILHHVGIHGELWATSHRRIFVRQHATWLPVA
                     QFPFHAPRDLFGFSRPSARVFRADKCNIYVNLLGNILGIRAGWAYRVKQNLAPQPLFQ
                     IQGDCVLHGSLCEDDAGNIYFGEYFMNPMRDAVRIWRVSPDLERWAVAYEFAPKSIRH
                     IHGVYRDPYDARAFWVAVGDFRGECFLMRTRDAFRTLERFGDGEQIWRAVKLFFTAEH
                     VCWLTDSNLERNHACRMHRRSGKLEIGMHIANSGWYGTTCREGLHVAFTTVERGPGIM
                     SNMSEVLVSRDAFHWETLHAFKKDWYRPLQLFKYGVISCPSGMMSQHELYISGEGLVG
                     LDGKSMRVDISDKG"
     gene            complement(12451..13203)
                     /locus_tag="PLL88_03020"
     CDS             complement(12451..13203)
                     /locus_tag="PLL88_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ00566.1"
                     /translation="MQRKLEIPGKEKPYLMAHRGNRAVCPENTLASFRHAFADGADIL
                     ETDLHLSKDGQFICIHDGTLNRTTDGSGVVADLTAAEIKKVSAFNRMAGFESEHVPLL
                     SETAHILPDDIALALELKTDRFLEEKVCRQLVAELKEYGVLDRTIVLSFSAERVLAMR
                     KVEKDMPIGLITMKRLIPPHDFDMVGPFFPIIWMNPWYVHTAHKKDLLVCPLDPAPEP
                     RLKHYLKRGCDAIITDDPAKTRQALNALLQRG"
     gene            13292..15526
                     /locus_tag="PLL88_03025"
     CDS             13292..15526
                     /locus_tag="PLL88_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00567.1"
                     /translation="MQEIPFLPAGDNSIRYPLKRFLPYFHVGVISSWLRQHAEAGGNV
                     LDPLGSNPLYALEAADDGFRIFQAQKNPVLRLMTEVLAQCFSESEYQKAVNLLLEQEW
                     HGQKLGRYIHDLYLTDCRNCGRSVAADGFVWKKDASDPTSVIYLCPHCGESGTFPTVE
                     ADLHRLEQIGNAAIYRSRAMQRCMIEGVDNRKNIEYALACYTPRALHVVVILFNTLDQ
                     LMVSQQERSIISAVLLEVFDQASSLWYWPSREFRPQQLSVPSVYFEKNISLSIQQALR
                     TWMDYRKSCEVVTYPVIPDQPRSICIFERKITSTLFAKQTDPNGFPTFCIFPRPNQAF
                     WVLSAMWSAWLLGKKAAEGMLSALARQRYGWYWFAQAISTTFGAIRNKIAKKDTIFGI
                     CSDFTPSYLLAVMLGANDAGFQLTGCAFQSNRNILQMTWRNSLQAEGPQPEPPIQPAR
                     DYLTQYLCERAEPATFQELFTVCCTAPAIHHEFLPSLEGESSEYFTKVLNDLQNELAN
                     TNVYRSFAGPNISSSRWMLVRTAKDIQPLDDRIEQAIVSTVQQNPSINFQMLYNELCQ
                     AFPGFQTPDRDFCRNCLESYATRAKTGQAIFMRDADEDARKRESELDEIKELICQIGR
                     KIGVAVHTNDGIHWMDAADKTLYTFYLSPTAIFSKYTQKENDGAHGTPVIVFPASRSR
                     LIQLKIERNPYLKETLSHGYHLVKYRHIRKISEQEQLTLQAWQDVLDVDPPLWDPPTQ
                     LKFL"
     gene            complement(15575..17308)
                     /gene="ptsP"
                     /locus_tag="PLL88_03030"
     CDS             complement(15575..17308)
                     /gene="ptsP"
                     /locus_tag="PLL88_03030"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOJ00568.1"
                     /translation="MKKLTGVAASRGICIGPAFQFQRSELDVQKIEINDPDVEIARLD
                     AAIESAKGQIDSIYQKALSEASEADAEIFQAHKMILDDPEFLYAIKNRIQCERINSEF
                     VVGETAKQFAATMAAMEDEYFAARATDIMDVSNRLIRVLLNVAESPTAGLKEPSVILA
                     DDLTPSDTVLLDKSKVLGFCIAQGSATSHTAILARGLGLPAVAGAGANVMNVRTGAPT
                     IVDGSNGVVLVEPDQETEKQYRQHMDTSKQIQSEALKFARSPAHTQDGVQFEVVANIG
                     NVEDAKFAVENGAEGVGLLRTEFLYLERNALPTEEEQYQAYKAIMDVFGKQPVVLRTL
                     DVGGDKEIPYLHLEKEMNPFLGERALRLCLNRPDIFKPQLRAALRAGVGHNLKIMFPM
                     VATVSCVRKALAVVNECKEEMRRENVPFAEDAEIGIMVEIPSAAVTADQLAQEVDFFS
                     IGTNDLSQYTMAADRTNPKVAELSDAFQPAVLRLVKMVIDAAHAEHKWVGMCGELAGE
                     PLAVPILIGLGLDEFSMSPPFIPLVKQIIRNLRTDEMVNLALAALQFTHPKEIQDFVK
                     QEVPFITEIIS"
     gene            complement(17305..17592)
                     /locus_tag="PLL88_03035"
     CDS             complement(17305..17592)
                     /locus_tag="PLL88_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOJ00569.1"
                     /translation="MKEAKIVVNNKVGLHARPAALFVQTASKFQSEINVSCVDPKESK
                     LRTANAKSILGILTLGVFQGIEITIKAEGGDECEAVDSLVDLVKNNFGEEA"
     gene            complement(17731..21309)
                     /gene="amrA"
                     /locus_tag="PLL88_03040"
     CDS             complement(17731..21309)
                     /gene="amrA"
                     /locus_tag="PLL88_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOJ00570.1"
                     /translation="MIKKPALTHFERTTLLQIARTSIEAAVRNEPLPEIDLAALPAPL
                     QENGASFVTLTIDGQLRGCIGTLEAYQPLALDVREHAIAAAREDYRFPPLREFELELV
                     EIEISRLTPTQSLSYQHPADLPELLQPLEDGVVLQDGYHKATFLPQVWEQLPKPEDFL
                     THLCLKMGLSGDVWREKVLKVGIYSVEEFRESKKKEITENLSKPRGSSSMESTKQIPR
                     KDETRQIPVKRSAASSPIKEMGATILKNLRSALSHLRSSFEKWREDRKAAKGKPPGAG
                     KGKGCFPRVVVWFLLGILIIFVGFSIFLLVQYYAIASTLPSVEDLKARASQFETTRIY
                     DRNGNLIYEILDPNAGFRTYISLEDMSPYIVAATIATEDKDFYNNPGFDFFGIVRALW
                     QNYTSGEIVSGASTITQQLARTLLLSPEERNQQTVQRKAKEIILAAEITRRYSKDEIL
                     ELYLNEIYYGNLAYGIQAASETYFSTSADQLNLAQASFLAGLPQSPAIHDIFTNRQST
                     LLRHQDVVNLMYQLSAEESCIEVSNSAAPVCVSMEEAVAAVLEIEKYPFQQKQINIPF
                     PHWVTYIRALLEEQFDAQTIYRSGFNIYTTLDPDLQNYAQKAVREQVAQLVENNAGNG
                     ALVAVQPRTGEILAMVGSSDFNNETIDGQVNMAVAPRQPGSSIKPLTYAAAFEKGWTP
                     STLIWDVKSEFPPSGNANDTRDPYIPVNYDSKYHGPVLVRTALGSSYNIPAVKTLQFV
                     GIYDDPNTPEEEGFIKFAERMGITTLTRSDYGLALTLGGGDVSLLELTSAYAIFANQG
                     VRAEPFAIAQIVDHQGNVVYEHELKTEQVIGSEYAYLISDILADNAARTPAFGANSIL
                     RLPFKASVKTGTTNDFRDNWTLGYTPDIAVGVWVGNADYTPMQNTTGLTGAAPIWADV
                     IQYAVQRYQGGAPSSIPQPSAIITEIICSVSGTRPSEKCPSEKSEIFNNSQPPLSADH
                     DLWKEVNLDTWTNKEASIFCSEFNEEKWTLNVSDDWGRKWITDTSAGRAWAEEMGFSD
                     PVIFTPDEQCSESDSQPTIEFVGLNNGTIITENPLQISVVIDVPSHFKNFKLLYGEGS
                     DPHSWKVLIEDGRSTYPDATVIYTLDLYEVGSQTITLRLFVENDMQGYAEKKITISFA
                     LPTPTPTITPTPTETPTPTPEVTATATEAVLPSETPTETPISTGAP"
     gene            21396..22151
                     /locus_tag="PLL88_03045"
     CDS             21396..22151
                     /locus_tag="PLL88_03045"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ00571.1"
                     /translation="MMRKDPETQPTLEKSTSPSLFNTGRVLQTVILVAAVLATLFTLW
                     NPYKLFSQKEVAQSAYQEDLANSSADSSTIGILVGHWKVDSGHVCDNGVVEANITEAI
                     SNQVSIKLNALGYPVKLLAETDLDLVNYRGLVLVALYTGSCDDSAENKSGFTIGTTLS
                     TTDLNSSNALAVCMGEVYQASTKLDFSYQIISPDQPAYTYFERVNRNTPMIYLEMGSL
                     LHDRSLLIENSDKVANGIVNGIQCYLDTLEKAE"
     gene            22148..23173
                     /locus_tag="PLL88_03050"
     CDS             22148..23173
                     /locus_tag="PLL88_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HOJ00572.1"
                     /translation="MNDTTPHANTISVMLEKAQSALKAGDKKEAHTWAEKILATAPEN
                     VDAHLILAAIGSPKESLRHLTRVLELDPQNKKARNGMRWAIAQAKLEKEQDKQTSSKE
                     DTQKIKVKASSTAQKTRKKKAQKHTAIWAILLFIAIVTGGLLAFSWYGLPLLKSASKE
                     VKEPRPAGVVIKPTLTPTPTSTPTPTLTPTSTLTPTPTPKPTENRTYLDYFAHSWDIP
                     DEVSGTDKFWIEVDLSEQMVYTYRGDTFLQSFLVSTGTNAHPTVTGTFKIYAKYPTYL
                     MVGDGYYLPDVPYSMFFYKGYSLHGTYWHSNFGTPMSHGCVNMVTSDAKWVYENAPIG
                     TYVYVHN"
     gene            23192..24235
                     /locus_tag="PLL88_03055"
     CDS             23192..24235
                     /locus_tag="PLL88_03055"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOJ00573.1"
                     /translation="MKKSISRRDFLKYSVQGAMGGFFYLHAFPKTFTLFDADASAPLM
                     GRIFKDNTVIYQEPSFSAGVKYHLTANSVLPLAGEVIGDPIHAHSKLWYEASDDGYIP
                     ANSLQPVRQHTNSPKTKINSAGELGTVTVPYTKAWLADPSSAPEKYLLFFYGSTHWVK
                     KVYTDAKGDTYYKIIEDRWGDAYYVQAEHVAIMQSEELAAFADAVPDAEKLLEVNIAD
                     QTVSAYEYGKMVFFSLASTGVLTEEVDLSTPPGEYSINYKRPSRHMVHTDKSGGDDGE
                     LYGVPWVTYFTNSGIAFHGTYWHNDFGVQRSHGCVNLPIPAAKWVYLWSRPAVPFGQE
                     KFVSNTGTKVIVS"
     gene            24314..24730
                     /locus_tag="PLL88_03060"
     CDS             24314..24730
                     /locus_tag="PLL88_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00574.1"
                     /translation="MSQRAVFEGLVIDENDRPVSTEYVGTEATYVVDDDGFMRHIPSE
                     YVDRQVLNFLKDQILQNKDILTDQATKMLGQEDIFTRAVIENQLEDVDKQMDVLLNTG
                     LPASNREYLGMVGFRVRIDVHGDILEIVQPAVSDED"
     gene            24785..25618
                     /locus_tag="PLL88_03065"
     CDS             24785..25618
                     /locus_tag="PLL88_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ00575.1"
                     /translation="MTSEFIIHANELNFEYEVISFSMNTPVVVDFWAEWCQPCKTLTP
                     ILERTILESGGSIRLAKVNIDQNPNLAVQYGVRSIPTVKAFVQGAVVAEFVGSQPPDR
                     VRAFLEQLTPPGPTDLAIEKASNLLILGNWAEAETIFRDALSSKPNSTSSLLGLSKSL
                     LAQGKADEALELLREFPASREYSRAQALMPLAESLRDFMQNRLLDENENDATFRNSLR
                     LISKGNMAAAMDGILGILRRNKRYQNGKAHQMALAIIEMLGEENPLTAQYRRELASAL
                     F"
     gene            complement(25634..26527)
                     /locus_tag="PLL88_03070"
     CDS             complement(25634..26527)
                     /locus_tag="PLL88_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HOJ00576.1"
                     /translation="MRKKLLLVVLLMGSLVACNFPFSKAAEPTSIFDQPAQTLTAMFA
                     AFPTPTSEVTGQALSPSSTVSSSSSTALSPSAPAILERSAQRVVAKYLSKKPVLDGDW
                     GDWKQNTIAYPAYYVVYGLKAWENDSDCEASFILGWDEDYLYVGVKVKDDTYVQNASG
                     DQIFRGDSIELLIDTDLQGDFYSDSLNEDDFQIGIAPGRGSISGPKEAYLWYPLSKKG
                     SISDQIEIASTSTAGIYRVEARIPWSLLQISPEKGLRLGFEFSVSDNDNPAEIVQQTL
                     VANIATHAFLDPTTWGELVLD"
     gene            complement(26706..27002)
                     /locus_tag="PLL88_03075"
     CDS             complement(26706..27002)
                     /locus_tag="PLL88_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00577.1"
                     /translation="MKRIIVWMSRVLLAYAVAGLTYGCTGRISKAILGKEEVFSPLLS
                     LPLHVVGWPWMVYADLKHVGVQLQDVLALVALVVCVVIMGKKAADSNATERKKS"
     gene            complement(27018..27578)
                     /locus_tag="PLL88_03080"
     CDS             complement(27018..27578)
                     /locus_tag="PLL88_03080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOJ00578.1"
                     /translation="MECVQTRLYKPGCDEHFVLHNLRNSPNFITRLDFVAELDGKVVG
                     NIVYSRCSVVSDIGKTQEALTFGPVGVLPAYQGQGIGSLLIRNTLQLAGQMGYKAVLI
                     FGDPAYYHRFGFEAAQKFAISTAEGENFDAFMVKELTAGSLQGISGRLFLDDVFFNID
                     QDELERYDSTFPPKEKRVTDTQLTKG"
     gene            complement(27735..28481)
                     /locus_tag="PLL88_03085"
     CDS             complement(27735..28481)
                     /locus_tag="PLL88_03085"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ00579.1"
                     /translation="MMDKFYDMIFKRKSIRKFDDALSISEEEMELIRQQIKALVPLEK
                     DIRVSFKIVKREETSARFGEYCLLLYSEQKPRYLLNAGYMLEQMDLFFASLDIGACWV
                     GMAKPEKLQNDGLEYVIMLAIGKSRPEDFRKDLSECKRKDSSMIWQGEFDAGVTDVVR
                     CAPSAGNSQPWQVVSSDHDIKIYRDTSARSFIMPKKKLHYFNGIDMGIFLYFLEVVLE
                     HRQMAFTRKLSDESDADTEKIAVAEYHILQ"
     gene            complement(28583..29119)
                     /locus_tag="PLL88_03090"
     CDS             complement(28583..29119)
                     /locus_tag="PLL88_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ00580.1"
                     /translation="MKIPHSERQKMVELIPYSDTYEEETVKRIADFFGYHSSLLNIEK
                     KEAEESITEARDTLREWQAEAHEFYIIVFQGKRIGFLHLWYKGPQVAWIEDIYVDEEY
                     RSQGIASHAIRKAEEIVKDHPDYTAVCLDVVPRNENALRLYYKLGYDSVNIITLRKEL
                     GENKRDRIEKIFGLKFKI"
     gene            complement(29116..30360)
                     /locus_tag="PLL88_03095"
     CDS             complement(29116..30360)
                     /locus_tag="PLL88_03095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOJ00581.1"
                     /translation="MNALTMHDVVVERMAYGGDGLARLVDGRVVFIPAVIPGEKVSVR
                     VVDDKQKYARAEAIELLAPSAERVSAPCPHFGVCGGCQYQHIQYHHQLRYKQDILREQ
                     LARLAHLDNVGEITVFPSDLAYGYRNVLEFHPLPDGKMGFMKRDSNAVFEVTRCLLPG
                     QTIQSAWSSLILEPGSEVTRVELRQNCAEELLVTLEGAPGSIPEIEVSAPVNMVHCSG
                     GETIVLSGQDALTMWVRDVPFQLSAGSFFQVNDGVAESMVSYITTIVQQLTPKKVLDL
                     YCGVGLFSRFIAPLIDELVAIESDPRACRNFAVNLDAFDNVSLYEGSAETIMPGLPFK
                     ADLVLCDPPRAGLHAHVIDALVHSGVTTLIYVSCDPATLARDVQRLGGNDFGVEKIAL
                     FDMFPQTYHFETVALLRAAGKE"
     gene            30523..31476
                     /locus_tag="PLL88_03100"
     CDS             30523..31476
                     /locus_tag="PLL88_03100"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOJ00582.1"
                     /translation="MRKQTIYTITSTFLFAALVLSACGGNAEAEATPTMDTESVIATS
                     IAQTMQAISETQTAQVTPTLLATATSAVAPTITPATGGSPTATVQSVSLPTNCLIAGL
                     VSETIPDGTVIARGASFTKTWSIRNGGTCTWTTSYKMVFESGELLGAASESVALTQNV
                     SPGMMTSVSIKMTAPSVDGTYIGYWNLLTDAGVLVGRFSVNIYVGTATAAPFAVTSVT
                     YPKSFSNLTCDGSTLNSIPVYITTDAAGTVTFTLTDLNGVSPTGAGDKDFGSAGTQTI
                     YFNMKFPSKASGQGVDVDISVYIDNPNHQTFLLKDSDGVCK"
     gene            complement(31537..32307)
                     /locus_tag="PLL88_03105"
     CDS             complement(31537..32307)
                     /locus_tag="PLL88_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HOJ00583.1"
                     /translation="MDGIEIVTLTPENAGQYGLCGYKDAKKHAELQRKIQWMEAFQPK
                     GLTIKVITIEGGGTQGMIEYIPGEYAHRPVNAQKYLFIHCLFVGFRKEYKRRGLATLL
                     LQECIQDACAQGRSGVTVVTRQGSFMADKPIFLKNGFHVVDQAAPDFELSVLKFNAEE
                     PDPAFKLDMADRLAAYAKGLTVFRSAQCPYSVKNVDAILASAEQMHIPTRLIEIEDAQ
                     TAQQVPAPFGSFCIVYEGKVIADHPISHTRFMNIMQKM"
     gene            complement(32427..32513)
                     /locus_tag="PLL88_03110"
     tRNA            complement(32427..32513)
                     /locus_tag="PLL88_03110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32475..32477),aa:Ser,seq:gga)
     gene            32623..33039
                     /locus_tag="PLL88_03115"
     CDS             32623..33039
                     /locus_tag="PLL88_03115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ00584.1"
                     /translation="MVSIRKMKVSDYDQALALWKRTPGMGLSAADEKDEVRKFLKKNN
                     ALCFVAFAGRELVGTILCGEDGRRGYLYHLAVDANCRKTGIGKSLVQKSLQALQKRGI
                     QKCHLFVIAENAPGIAFWKHTGWQLRDDIEVMSIDL"
     gene            33285..34082
                     /locus_tag="PLL88_03120"
     CDS             33285..34082
                     /locus_tag="PLL88_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00585.1"
                     /translation="MKNQFKQETWMGVLCGCVFAAVAWGYDGYLLSKAHAALPWLKLA
                     LGLPPCAAVGGLAGWLTMKMSNMFTRMALWVAVAIFFSYLTSLIPFTLSPIIYRWSFP
                     AVSERIIYNASVAISARRFITIVMTIVFLFIAGLIFENVNDSVRNARGIVGVVFSVIF
                     LLAFFAGAGYTADSNFNVDLRTPILALDEKLDYVASVNAATLSDVEARAIRRFTKLDV
                     DLQSPRRTVIATFDEYISQMIVVVDFDGTLAECSVMNGRASSCKMVE"
BASE COUNT         9021 a         8989 c         8252 g         7905 t
ORIGIN      
        1 gaaaagatcg atgtcattga atggaacaaa gacccgcgca tttacattgc caaagctctc
       61 agcccggcgc gcgttaatgg tgtgtattta aacgaaaccg acagcaacgc aaaaacagcg
      121 ctagtcgtgg ttcctgaaga ccagctttcg ctcgctatcg ggcgggatgg gcaaaacgca
      181 cgtttggctg cccgtctgac cggctggaga attgatatca agagtctgcc cgaggcagtt
      241 tctgactggc tgttcgcctt gcaaaacaac gaagaatatg ctgaaaaagc ggagcagttc
      301 aaagaagaaa tagccaaaac cgaggatatc atggctcgca agaccgaagg gcggatcctc
      361 aacatggaag actatgatag ccttgccagg ttcgttgacc ttatggaaaa agggctcttt
      421 gctgaacggc aagaggtcat tgcagctcgc gaaaaagcca tacaggaagc gttacaaaca
      481 attcccaagc acgcttttag cgtgccattg agcgaagaga tcatcccgca aaccatcctc
      541 agcattttgg gcgacagcgg tattcaaaat ttgggcgatc tggcgttgga gctcaaatcc
      601 aatcccgatt ccattctggg actccccggc attggcccga aagcgcttga aaagatcgaa
      661 agcgttgtca atgacctgtc cagattcatc cctgccgagc cagaacccga ccatgaagta
      721 agcgaagctg taacagtggc agatgagttt gttgaaccgc aagaggctgt tcaggaagca
      781 tccgaaacaa tggacctgcc tgaagaaaca acgccagaaa gagaagcgga acagcagccc
      841 gagcaggaag aagggcaaga actctctttt gaagaaatgt tcaaaatcga gacccaaaaa
      901 accaatggcg gatcgttcga ggatgaagac gatgaactcg ctgataaaag cggaaaaaag
      961 aaaaaaaaga agaaatatca agagatcgaa tatgatcctg atcgtgatat gaccctggtt
     1021 aaaaagaaac acaaacgcgg aagcaatgac tggggtctgg atgacaacgg taactagaaa
     1081 aagagaacgg tggtgaaaaa gcaaaagcta cgccaaaaac acattccaca acggacctgc
     1141 gttggatgtc gtgaggttct ggcaaagaaa gaactgatcc gcatcgttcg cacgtctgaa
     1201 gggatcttgg tggatccaac gggaaaattg aatgggcgtg gcgcttatat tcaccttcaa
     1261 aaagagtgtt gggagcgtgc gctgaaagga agtttgtcga aagcattaag aacagatctt
     1321 agtgagcagg atagaaaata tctggaagct tatcaaatga acctgccgga gagcaaatca
     1381 aagatgcagg attaatcaca gtcttccact cagataccct tgctcgcggg aacatcctag
     1441 taatcaaata gattcagggg agcagcatca acggaggtaa aaatgagtga aaacggaaaa
     1501 aagactgtgg agctacctgc cagtataacc atcagagacc tgggagaacg aattggcttt
     1561 agcccaattc aaattatcaa gatattaatg tccaacgggg tgatggcaaa tatcaatcaa
     1621 atcatcgatt ttgacacagc cgccattgtg tgcgctgaac tgggcatcga cgccactctt
     1681 gaagtcgctg aaatcaaaga agaggaattg ggtgaggtac ccctatggag gaaattgatc
     1741 gaaaatgagg accccgccaa ccttgcaaag cgacctcccg tcattaccat cttaggtcat
     1801 gtagatcatg gaaaaaccac cttattggac gccatccgcc ataccaacgt ggcaggggaa
     1861 gaatcgggtg gaatcacaca gcacattggc gcatatcaga tcgttcataa caatcaaaag
     1921 atcacttttc tggacacccc agggcatgcg gcatttactg ccatgcgcgc tcgtggagct
     1981 cagggggcag acatcgtcat tctggtagtg gcagcgaatg atggcgttat gcctcaaacc
     2041 aaagaagcca tcgcacatgc caaagcagca cgagtcccca tcgtcgtggc tttgaacaag
     2101 atcgatcgcg atgatgccaa ccctgacatg gtcaagagac aactggcaga tgcggggctc
     2161 gttcccgacg attgggatgg agacaccatg gttgtgccgg tctcggcaaa gaccaaaaaa
     2221 gggttggatg atttgctaga agccatttct ctggttgccg actcttgcaa gattttggca
     2281 aatcctaagg ggaaggtgat cggtacggta atcgaagcta aggttgacaa agcgcgtggc
     2341 gtggtcgcca cgttactggt gcagaatgga acgcttcgcc agggtgaagc agtggtagtt
     2401 ggtgttgcac acggaaaaat caaagccatg tacaatttct tgggcgagaa gatcgaagaa
     2461 gccggtccct ccacccctat tcagatcctg gggcttaacg aagtaccaca ggctggagat
     2521 atcttccagg tagcggcatc cgataaggat gcccgtgcca tcgtcgccga ccgcaaggca
     2581 aaacttagta aagcaagaca aaacaaaaaa caagccagtt tggaagacct gttcaatcgt
     2641 tttcaagcag gcgaagtgcg cgaactgcgc ttgattatca aagcggacgt tcagggatcg
     2701 ctggacccca tcatctctac cgtcaatgag atcaaccaaa aagagatcaa gatcaacatt
     2761 ctccacgctg aaacagggaa tatcagcgag agcgacgtga tgcttgcctc cgcatccaat
     2821 gccatcattg tagggttcaa cgtagaagcg gaaacatcag caaacaaact ggctgaagtc
     2881 gagggcattt ccattcgact ctacgatatc atctaccgac ttaccgaaga tatcgaaaaa
     2941 gccatgaaag gtatgcttga acctgaaaaa caggaagttg ttcttggcaa agcgcaaata
     3001 ttggctgtgt tttccttctc aaaagttggt aaaatagctg gctgtaaagt aaccgatggc
     3061 gtgatccgcc gcaacgcgaa tatgcgggtg gtgcgcaacg gtgaagtgct cgccgacaac
     3121 gagatcgcct ctttgcggcg cgaaaaggaa aatgtacgcg aagtgcgcga agggtacgag
     3181 tgtggaattc gagtgaaagg attcaacgat tttgccgaag gcgatacttt gatctgttat
     3241 accttcgaag aaaaacaggc aatttaaggg aaaaaataac aggaaatgcc atccataaca
     3301 agattgaaaa ggattgccga ccgcatgcag caagaccttt ctgaaatgct ggttcaaggt
     3361 aatatccatg acccgcgact cgaagggatt ttcatcaccg atatccgggt tgaccgcgaa
     3421 ctcgcatacg ccaacatttt tgtctctgcc cttgagggaa aagcgcggga agaagaaatt
     3481 cttgaaggat tgaagcatgc caggggtttc ctgcgcaaag aactggcaag gcggatacaa
     3541 ctgcgcacgt ttccagagct gcgcttttat tgggatccaa cccccgagcg cgcggagcga
     3601 attgaagaac tgatcgacac gctccagact gaaagggaaa agaaggaaca gaagaatgac
     3661 cagggatcca gcggctagcc agcgcatctg gcaaaagatc gatgcagcag aatcgatcat
     3721 tttggttacc catgtgcgac cagacggcga cgcagtcggg tctgtgcttg gttttgcaga
     3781 agccctgcgc gggtatggca aagaagttca tattgccttt gaagagactc tgccagtgcg
     3841 attccgtttt ctggaagaaa aagaaaagcc caccagcatt tcgacgagtg gattgaccgt
     3901 tacggtggat tgtgccgacc aagcccgcat gcccaagatt tttgcgaaaa aaaagatcga
     3961 tatcaatatc gatcatcatg ccaccaatac ccaattcggc gacctcaatt atgttgatgc
     4021 agaagcggca gccactgccg agatcatctc tgatcttctc ctcgattgga atatccccat
     4081 cacgtcttcc gcagccagcg cccttttgat gggtatcatc accgatacca tcggatttcg
     4141 aactcccaac acaactcccc acaccctgcg caccgcagcc aaattatccg aactgggtgc
     4201 caatctcagc gagatatacc agcgctcatt aaccgatatg acctgggaag cgagtttgtt
     4261 atgggggttc gcgctaaata aacttcaaaa agaagggaaa atcatatata ctgttattac
     4321 ctgtgaagat cgtaaaagat ccggattcac agacagagat gatgccgatc tcacgaactt
     4381 tctcagcagt attcaagacg cagagatagc gatacttttt aaccaacaaa gtgaaaaatc
     4441 ggtgaaagtt agctggcgct ctcgttcaaa ggtaaatgtg gctgatctgg catctcaatt
     4501 tggtgggggt ggtcacccgg ctgccgcagg agcggaaatg gaactttcac tacaagaaac
     4561 ccagcaaaaa atcttagaag caacaaaagc ttacttatta aagatagaat aatcaggagt
     4621 gcatgaatgg acgatagaag aactcaactc aaaaatactg tgtcaggagt cctggtagtc
     4681 gataagccaa tcgggatgac ctcgcatgat gttgtacaga tcattcgaaa cggaaccagg
     4741 atcaaacgag ccggtcacac cggcacgctt gatccgcgag cttcaggcgt tcttgttatc
     4801 ttattgggac ctgccgtgcg tttgagtgag tatgtatcgg ctgaagataa acagtatcac
     4861 gccgtgatcc agcttggaaa gaaaacggac acgttcgacg aagaaggtgc ggttaccggt
     4921 acttctccgg tagatatcac cgccgagcaa tttgaagaag ctctgaaaag tttcatcggt
     4981 gaaattgaac aggtcccacc accctattca gccgtaaaag tgaacggtcg caaagcgtat
     5041 gaatttgctc gagaagggga agatgttgaa ttggaacccc gcatcatcca ggtctacaac
     5101 ctggaacttc tcgaatggga cccgccccaa gcactggttg acgtgcactg ttcttcgggc
     5161 acctatgtgc gctcgctagc caacgacctt ggtgaaaaat tagggtgcgg ggcaatgtta
     5221 gcaggcttgc gccgaaccaa aagcgggaaa ttcacgctca aagacgccac acctttacgg
     5281 aagttgagcg aggcattcca ggatggaacc tggtataaat atctcatccc cgccgccgac
     5341 gcgctgggag aatggccaac catctatctc accgcagaag aagtggagct cgtacagcac
     5401 gggcaccgca tccgccgtga tgaaaagatc ggcgacatgg caagggcaat cacggaacag
     5461 ggtgaactgg ttgccttgat gcagtatgat gaagaaagtg gagaatacca accgaaaaaa
     5521 gtattctttt cgtagatgtg cccaccatta accatccatc actctcgcaa ataaccattg
     5581 gatcgtttga tggcttgcac gcaggtcatc aaacgatcat ccatcacctt gttgcaggcg
     5641 cgcacagcca ggggcaaaaa gctgttttgg taacctttta cccgaacccg accgtgtttt
     5701 tcaaaaagat acacacccct ttctatctaa ccaccaacag tgaaaagata gccctcttgc
     5761 agaaactggg gattgatgaa accatcatcc tgcctttcaa ccaggaactt gcacaatcca
     5821 gtgcagagga tttcatcaga tacctgctgc aacgggtgcc attcaataca atgtatatcg
     5881 gatacgattt tcacttcggt gctgaccgcg caggggatag ggggaagttg ttcgaactgg
     5941 gaaagaagta cggtttcacc gtgcaggtcc aacaacccgt agacctcaac gctaccccga
     6001 tctcttccag catgatccgc agcatcattt ccgaaggaag ggtcgaggat ctttccgctt
     6061 tgctcaagcg ctggtattcc atggagggcg aagtgatcca cggcgatggg agaggaaaga
     6121 aaatgggcat tccaaccgcc aacatcgcct acgaacccca gaaactgctg ccacccaacg
     6181 gcatttatgc aacgcgcatt caaatcgggg tggaaacccg cccggcagcc accaatgtcg
     6241 gttttcgacc cactttctac gaaactcctt ctattaagac tgttgaagcg ttcatcctgg
     6301 attgggagtg cgatatctat ggaaaaaccg taacgctgga gtttgtacgc aggttgcggg
     6361 atgagataca atatcagaat acccaggcac tcaccgctca gattcggaag gatattgcat
     6421 taaccaggaa aatcattgat catgagtgaa aaacggcgcg tttatctcct cgataataaa
     6481 aaactcagtc ctgaaacaat cgccgttact tttgccaaaa cctcccgctc cccacaatct
     6541 ttcgaccaga tagcccaaga gctttccgat gaaaaaagcg cgcaattcca cgagcgctgg
     6601 gtggttggtt acgggcacgc atcggtggca gagcacgcag ttctgcacat tgccattgag
     6661 aacgtttcgc ggttggcgat cgaatgcctg gaatcaaaca ggctggcttc ttacacagaa
     6721 aaatcatccc ggtatcaaac ctgggatgcg gattgtttct tcatacccga tgagatcaaa
     6781 ggcacttcgc tggaagatgc atacagcaac acaatcaacg ctcttttcca gaagtatcac
     6841 aatgcgatcc cctttctcca aaccaccatc gccaatatct gccgggctac aagcaagcca
     6901 atctctgata gggaaattca tgcgcgctgt atggatgtgt gccgctatta tttacctgcg
     6961 gctgccatgg caaacgttgg cgtgaccatc aacgcgcgcg agttggaaca cgcactgcgc
     7021 aaaatgcttt ctcactcgct gctcgaagtg cgccagatcg ggcaggagat caaacaggtc
     7081 gccatcagcc acctgccaac tctggtcaag tatgcggatg aggtggctta tttcaaacaa
     7141 gctgaagagc ggaccaccca attgatcgcc aacgcacacc cagccagcca cggcaaaaat
     7201 tggtgcacgc tgctcgatca cgacgagcgg ttcgagcacc acattcttgc agcgctgctg
     7261 ctgcgctatg ggcagggaga ttatgcagcc caagaaaaca agttgcacga tttttccaag
     7321 gaacagcagc aaactctgat ggatattctg ttcggcagat tggacgcaca cgccatgccg
     7381 ctacgagaac tggaatatag ttttttcctg tttgagctgc tcatggacca gggcgcttat
     7441 tttgaattca aacgccaccg catgatgacc caaaccgccc aggtcttgac cccctattat
     7501 ggttatgcca ttccccgctt gatcagcgac gccggcttgg aagccgattt tcacgaagca
     7561 atggcaatag cgcaagaagg ttacacgcag atcgcagcaa cccaccccgc tgtggcgtcc
     7621 tacgtcattc ccaacgcata taaccgtagg ctgctagccg gttgcaatct gcgcacagtg
     7681 attcacttca ccaacctgcg cactgcctcc aacgcccatt ttgccatgcg ccgggtagcc
     7741 aaccgcatgg cagaagagat cagagagcgc atggacctgt tcaaaccgta cttctatgcg
     7801 gatccacccg aaacctggca gcaaatcgaa acagaatttt tcaccgatcc gatctaccca
     7861 taagaaccct tgcttctgtt cgcaaatttt tcgattcttc ttttttctta tacaatagag
     7921 agaaagatca ctccatgcat gtttatggga aagagggttg aatgagcaaa attgaatttg
     7981 tcatcaagcg aaatggggca accgtgccct ttacccccca acgcatcagc aatgccattt
     8041 accgcgccgc agtggcggtt ggcgggcggg acaaagaaac cgcggaaaaa cttgcccttc
     8101 aggtggtcga atacctggaa cagaatttcc cacccacgaa atatccaagt gtcgaagacg
     8161 tgcaggatgc tgtggaaaag attctcattg agaacggtca ttccaaagtc gccaaagcct
     8221 acattctcta tcgtgacgaa cgagcgcgca tgcgttccag ccgcaaagac caaaccatcc
     8281 aaccttcgca aaacatcccc tgggcgaaaa tctggcaggt gctggattgg gcttcgtcac
     8341 gcgacctgca cactgtagag cgctacaacc agcgcatccg aaacggcgaa gcctctgaaa
     8401 tcatccgcga ctccgagatc ttttacgaag aagatatcaa gatcgcagcg cgcatggtgc
     8461 tcgaacgccg cgaccaggta aaggttgtgc tgatcacagg tccctcttcc tcaggcaaga
     8521 ccaccacaac catcaaactg ggaaacatcc tgcaacagag caacatcaaa ctggtaactt
     8581 taaacgtcga ccattatttc tttgacctcg atgaccatcc gaaagacgaa ttcggtgact
     8641 acgattacga ggcaccgcaa gccctcgacc tcaacctcat cagcgagcat ctcgcaactt
     8701 taatcacagg aaaagaagta aagatcccat tctatgattt caaaaccggt aaacgtcagc
     8761 tcaaccacac gcctatgaag ctaaaagaga atgagatgat cctgatcgac agtctgcatg
     8821 gtttatatcc cgctctaaca gagggggttg aagaaaaccg caagttcaag cttttcctgg
     8881 aaccgttatt acagatgaaa gatgatgagg ggaaatatat ccgctggacg gacatccggc
     8941 tcatgcggcg catgctgcgc gatgctgctt tccgcgctct gaaccccgaa agcaccctca
     9001 cccactggca ttacgtacgc accagtgaat tgcgcaacat cattcccaat atcaatcgcg
     9061 ccgattcgat catcaacagc gccatgccgt atgaactttc tatttataaa cccaagttgc
     9121 tccatctctt tgaaaaatgg ccagaaaaat tcaaggacga tccattgcgc caagatgcct
     9181 acgaacgatc cagccgtatt ctcaccttgc tgcaaaagat cgaaccactg tccgatgatt
     9241 ccattgtgcc cagcgattca gtcatccgcg aattcattgg cggcagcagc ctgagctatc
     9301 attaagccat gccatacgcg gtcgtgctat atctggatgc gcaggcatcc caccccattc
     9361 ggcaagccat tcaggagttg gctttaaagg acgtggctcc atacatggaa gtgcattctg
     9421 tccaacccca cgtcaccctg gctatctatg acaacctgga ctgtcagcta tgcgaaagaa
     9481 agatcgcaag ggctgcccgg attatgcggg gacttaccct gaactttgcc tatctgggtg
     9541 tttttcactc agagagcgtg gtggtctacc tgggggcgac ccctacgcag gaacttctgg
     9601 caatacaaaa acgcgtgcat ggaattttga acagcatcgc ttccaatcca tgggatctat
     9661 acctgcccgg caaatggatc ccgcattgtt ctctggcgat tgagctttcg cctcgtaagt
     9721 tgcaaaaagc gatgcaagtc tgtatggaaa ttgcactgcc gctgaccatt tccatcgtgt
     9781 ctcttgggat catgcaattc gaaccgcttc agccaatata cacgtacaca atccacggtt
     9841 ataaaataat aaaagaataa tcctacgaat tccgtaagga tttcttcaga ttttcctccg
     9901 ctacggcttt caagcgcggg tgcatgacaa agtattgcct tccagccaat ggcgtctgcg
     9961 cgtgagcatg cagatcccct gacacaatcc cgcgcacccc ggcaagcatg accctcacca
    10021 tttccacttc caaacgcatg cgaatggtgg aaaaggtatc cccttcaaca gggtcaaacc
    10081 gtttcaccag caagaccggt ccagtatcta cccctttatc catatagtgt aagctcaccc
    10141 caaaaccaac cttttggata gcgcattcca gggcagtcca ctccaccaca tccataccgc
    10201 gataggcggg caatatgccg gtatggcagt tcagcacgcc caatggaggg atttgcagaa
    10261 tattctgcct gatcaatcct cccccggtaa aaacgatcac atcgggagca tgctggaaga
    10321 caaactgtgc gcactcatca gcgctgagat cccggcaaac caggtaggga aggttccaac
    10381 tacctgccag ttcctttagg gtacccttgg gaagttgaac agatgccgca aacgaagcca
    10441 gattttccac tgctatgccg cgaaaagcgt tttcgcgcag caggtatttg cggataacct
    10501 tctgcgccag gcggctgcca tcgcgtttcc attcggagcg aatgcgctgc atattccagg
    10561 gcgaacggac cgccacgccg accacctgca ccccctgctc ccgcagtaag ccgtgcaaga
    10621 ccaggcgcga atacaggctg gtagatatgg gagcgaacag caggactttt aacatgacca
    10681 caatccctgc aggattctct cctgctccac cagcgcaagc aacagaggca aggctggcaa
    10741 tgcaggaatc gatcggcggc gctctttcca cagccattcg ctcaatttca gggcagcgtt
    10801 cataaagcgt ccgtcttttt tcagatcagc atagcgacaa aaaagggctg cgtatgcaca
    10861 gcgttctcgc caacgcagcg ttcgcgtaag cggttcggaa tagcgtcgga gagcctgctg
    10921 acgcttcacc tctacatgac gcagcagaac ctccatgttc gtctccagca agcttgagcc
    10981 cctctcgcca gattccaaca tgtcaagcag cacttcagcg cctgggtatt gcagaacccc
    11041 tttagtcagc acccgctcat tttccagcgt acctcgccgg tcatcttttt ctcttgcgtc
    11101 agtctgcgct tcgtcggcaa cgtgaaagcc tgatgcctcc atcttgtttc ccagcgaagg
    11161 caaagtctgg cagagccggc gcacctgctc tctttcgatc tcaggaaaac attccaaccc
    11221 tgatcgctgg acccgttgga ataccttaga actttctcgt tcgataaaag cgtcgatgcg
    11281 atcgggctgc aaaaatcgtt cagcaaggga attatcaagc gcaatcaaaa atttttgtat
    11341 gcttgccatt atcaggtcac cctttgtcgc tgatatctac gcgcatgctt ttgccgtcta
    11401 atccaaccag cccttcaccg cttatgtaca attcatgctg actcatcatg ccggagggac
    11461 agctaatgac accatatttg aaaagttgaa gcggtctgta ccaatctttc ttaaaggcat
    11521 gaagcgtttc ccaatggaac gcatcacggc tgaccagcac ctcgctcata tttgacatga
    11581 tgccgggacc cctttctacg gtggtaaaag ccacatgcaa gccctcccgg caggtcgttc
    11641 cgtaccagcc ggaattggcg atgtgcatac cgatctccaa ttttcctgac cggcgatgca
    11701 tacggcaggc atggttgcgc tccagattgg aatcggtcaa ccagcacaca tgctctgcgg
    11761 tgaaaaagag cttcaccgca cgccagatct gctctccatc gccaaaccgc tccagcgttc
    11821 gaaaggcatc acgtgtgcgc ataagaaagc attccccacg gaaatcaccc actgccaccc
    11881 agaacgcccg cgcatcatag ggatcacgat acaccccatg gatgtggcgg atgctcttgg
    11941 gcgcgaactc atacgcaaca gcccaccgtt caagatcggg cgatacgcgc cagatgcgca
    12001 cggcatctcg catcgggttc ataaaatatt caccaaaata aatattgccg gcatcgtcct
    12061 cgcacaacga accatgcaaa acgcaatccc cctggatctg aaataagggt tgtggggcaa
    12121 ggttctgctt taccctgtac gcccagccag cgcgaatgcc aaggatattc cccaagagat
    12181 tgacatagat attgcattta tccgcccgaa acacgcgcgc gctggggcgg gagaatccaa
    12241 aaagatcgcg cggagcatga aagggaaatt gcgccaccgg cagccaggtg gcgtgctggc
    12301 gaacaaaaat gcggcggtga gaagtagccc atagttcgcc atgtatacca acatggtgca
    12361 gaatggtcat gcgcgggtat tgttccacga tcgtcaattt catgggcgta atttctttcg
    12421 aatagtaagc gacaacgcta cgagcaagct ttatcctcgc tgcaacaagg cattcaaagc
    12481 ttgacgggtt tttgcaggat catccgtgat gatcgcatcg cagccccgtt tgagataatg
    12541 cttcagacgc ggttctggcg caggatccag cgggcacacc aacagatctt tcttatgagc
    12601 ggtatgaacg taccatggat tcatccagat gatagggaaa aaaggaccta ccatatcaaa
    12661 gtcgtgcgga gggatcaaac gcttcatggt gatcaagcca atcggcatat ctttttccac
    12721 tttgcgcatg gcgagaacac gctcagcgct gaaagaaaga acgatagtgc ggtcaagaac
    12781 cccatattct ttcaattccg ccaccagttg tcggcacact ttctcttcga gaaaacggtc
    12841 agtttttaat tccaacgcca atgcaatatc gtctggaagg atgtgggcgg tttcagacag
    12901 cagcggaacg tgctcgcttt caaaaccagc catgcggttg aaagccgaca ctttcttgat
    12961 ctctgccgcg gtcaaatcag caaccacgcc gctgccatcg gtcgtgcggt tcagagttcc
    13021 atcatggatg caaatgaatt gaccatcctt gctgaggtgc agatcggttt ccagaatatc
    13081 agcgccatcc gcaaaggcat ggcgaaaaga agccagtgtg ttttcagggc acaccgcccg
    13141 attgccgcga tgcgccatga gataaggttt ttcttttcca gggatttcca gttttctttg
    13201 catactaccg tccatctctg attttagcac cagaacgaac tgcaatcgct tgcattttcc
    13261 tgtatactac aaaaggagga tatcaaagaa gatgcaagag atacccttcc ttcccgccgg
    13321 ggataattcc atccgatatc cactaaaaag atttctcccc tactttcacg tgggggtaat
    13381 ttcgagctgg ctacgccaac atgctgaagc cggcggaaac gtcctcgatc cgctgggttc
    13441 gaacccatta tatgcccttg aagctgctga tgacggtttc agaatattcc aggcacaaaa
    13501 gaatcccgtc ttgcggttga tgaccgaggt tttagcgcag tgctttagcg aaagtgaata
    13561 ccaaaaagcc gtgaacctgc ttttggagca agaatggcat gggcaaaaat taggacgtta
    13621 catccacgac ctgtacctca ccgattgcag aaattgcggt cgttcggtcg ctgccgatgg
    13681 ttttgtatgg aaaaaggatg cgtcagatcc cacctccgtg atatatttat gcccccactg
    13741 cggtgaaagc ggcactttcc ctaccgtgga agcagatctg caccggctgg aacagattgg
    13801 caacgcagcc atttatcgct caagagccat gcagcgctgc atgatagagg gggtggacaa
    13861 tcgaaaaaac atcgagtacg ccctggcttg ctacactccc agagcactcc atgtggttgt
    13921 aattctcttc aacacactcg atcaattaat ggtttcgcaa caggaacgtt ccatcatctc
    13981 agcagtactt ttggaagtgt tcgatcaggc ttcgtcgctg tggtattggc catcgcgtga
    14041 atttcgcccc cagcagttat ccgttcccag cgtttatttc gaaaagaaca tctccctgtc
    14101 cattcagcaa gccttgcgca cctggatgga ttacagaaaa tcctgcgagg tcgtcacgta
    14161 cccggtcatt cccgaccaac cgcgcagcat ttgtatcttt gaacgaaaaa tcacaagcac
    14221 attgttcgca aaacaaaccg accccaacgg attcccaacc ttttgcatct tcccccgccc
    14281 caatcaggct ttctgggttc tctccgccat gtggtccgcc tggttattgg gaaagaaagc
    14341 cgccgaaggt atgctttcgg cacttgccag gcagcgttac ggctggtatt ggtttgccca
    14401 ggctatttcg accactttcg gcgctataag aaacaagatc gccaaaaaag ataccatctt
    14461 tggcatatgc tccgatttca caccctcgta cctgctggca gtcatgctgg gcgcgaatga
    14521 cgccggtttc cagctcacag gctgcgcctt tcaaagcaac aggaatatct tgcagatgac
    14581 ctggcggaat tccctgcaag cggaaggtcc ccaaccagaa ccccccatac aaccggcacg
    14641 agactatctg acccaatatc tgtgcgaacg cgctgaacca gccacatttc aagaactgtt
    14701 caccgtctgc tgcaccgcac ctgccatcca tcacgaattt cttccttcgc tcgagggtga
    14761 aagttcagaa tacttcacca aggtcctcaa cgacctgcaa aacgagttgg caaacacgaa
    14821 cgtttatcgt tcctttgccg gtcccaacat ttcttcaagc cgctggatgc tcgtaaggac
    14881 tgcaaaggat atccaaccct tggatgaccg aatagagcaa gccattgtaa gtactgttca
    14941 acaaaacccc agcatcaatt ttcagatgct gtataacgag ttgtgccagg cgttccctgg
    15001 ttttcaaaca cctgaccgcg atttctgtag aaactgcctt gaatcctacg cgacccgcgc
    15061 aaaaacgggg caggcgatct tcatgcgcga tgccgatgaa gatgcgcgca agcgagaaag
    15121 cgaactggat gagatcaaag aactgatctg ccagatcgga agaaaaatag gcgttgccgt
    15181 acatacgaac gatggaatac attggatgga tgccgctgat aaaactctct ataccttcta
    15241 tctatccccc acagccattt tttcaaaata cactcaaaaa gaaaatgacg gcgcacatgg
    15301 aacaccggtc atcgtttttc cagccagccg ttcgcggttg atccaattaa agatcgagcg
    15361 gaacccgtac ctcaaggaaa cgctgagcca tggatatcac ctggtaaaat accggcacat
    15421 ccgcaagata tcggaacaag agcaactcac cctgcaagca tggcaggatg tactggatgt
    15481 ggatccaccc ttatgggatc caccaaccca gttgaagttt ctttaaacaa aaaagggaac
    15541 cttcgttccc tttccacttg caaaggtcgt tgcgctacga gattatttct gtaatgaacg
    15601 gcacttcttg cttgacaaag tcttgaattt ctttcggatg ggtaaactgc aaggcagcca
    15661 gtgccaggtt caccatttca tctgtacgca gattgcgaat gatctgcttc accagaggga
    15721 tgaacggtgg gctcatactg aactcatcca agcccaatcc aatgaggatg ggcacagcca
    15781 gcggttcacc agccagctct ccgcacatgc ctacccattt gtgctcagca tgggctgcat
    15841 cgataaccat tttgaccaaa cgcaaaactg caggctgaaa agcgtcagaa agttccgcca
    15901 ccttgggatt ggtacggtca gcagccatgg tatattggct gagatcattg gtgccgatag
    15961 agaagaaatc cacttcttgg gctaactgat cagcagtgac cgccgccgaa gggatctcaa
    16021 ccatgatgcc gatctccgcg tcttccgcaa atggaacatt ttctcttctc atttcctctt
    16081 tgcattcatt caccaccgcc aacgccttgc ggacgcatga aaccgtggca accatgggga
    16141 acatgatctt gaggttatgc cccaccccgg ctcgcagcgc ggcgcgcagt tggggtttga
    16201 agatatcagg gcgattcaag cataaacgca aagcgcgttc acccagaaat ggattcattt
    16261 ctttttctaa atgcaggtag gggatttctt tgtcgcctcc cacatccagg gtgcgcagaa
    16321 caaccggctg ctttccaaaa acgtccataa tggctttata tgcctgatat tgctcctctt
    16381 ccgtcgggag ggcattcctt tccaggtaca ggaactcggt gcgcaaaaga ccaacgcctt
    16441 cagcgccatt ctcaacggcg aatttcgcat cttctacatt gccaatattt gcaaccacct
    16501 cgaattgaac gccatcttgg gtatgcgcag gagagcgggc aaacttcaga gcttcgcttt
    16561 ggatttgttt gctggtatcc atatgctgac ggtattgttt ctcagtttcc tggtcaggtt
    16621 caacgagcac cacaccgtta ctgccatcaa caatggtcgg cgcccccgtt ctcacattca
    16681 tcacattcgc ccctgcgcca gcgactgccg gcaaaccgag accgcgcgcc agaatagcgg
    16741 tatgtgatgt ggcagacccc tgcgcgatgc agaaacccaa caccttgctt ttatccagca
    16801 acacggtgtc ggaaggggtc aggtcatcag ccagaatgac agagggttct ttcagaccgg
    16861 cagtgggcga ttctgccaca ttcagcagca cgcgaatcag ccggttggaa acatccatga
    16921 tatccgtggc gcgagccgca aagtattcat cttccatggc agccatggtg gcagcaaact
    16981 gtttagctgt ttctccaaca acaaactcgc tgtttatccg ctcacactga atgcgattct
    17041 tgatggcgta gagaaattca gggtcatcca ggatcatctt atgcgcctgg aatatctcag
    17101 catccgcctc gctcgcctcg ctcagcgcct tctgataaat gctatcaatt tgtcctttgg
    17161 cagattcaat ggcagcatcc agccgggcta tttcaacatc cggatcattg atttctattt
    17221 tttgaacatc gagttcactt cgttgaaatt gaaatgctgg accgatgcag attccgcgag
    17281 aagccgcaac accggtcagt tttttcatgc ctcttctccg aagttgtttt tgacgagatc
    17341 caccagagaa tcgacggctt cgcattcatc gccgccttcg gctttgatgg tgatttcaat
    17401 accctgaaac acacctaggg tcaaaatacc caggatactt tttgcattgg cagtgcgcaa
    17461 tttactttct ttaggatcaa cacacgatac gttgatctcg ctttggaatt tggatgcggt
    17521 ctgtacaaag agtgcggcgg gacgtgcatg caatccgact ttattgttga cgacaatttt
    17581 ggcttccttc attatcttct cctgaaatca aaaataataa accgataaaa atcggcaaat
    17641 accatcgtta aacaatcccg caaccactct gggttacggg atgtaatttt atcacatgtc
    17701 atttatttca tcagaagtca tcacatttgg ttagggcgcg ccggtggaaa tgggagtttc
    17761 tgtaggagtt tcggaaggca gcacggcttc tgttgcagtt gccgtcactt ctggcgtggg
    17821 ggttggggtt tcggtgggcg tgggtgtgat ggtaggggtt ggcgttggaa gagcaaaaga
    17881 aatggtaatc ttcttttccg cataaccttg catgtcgttt tccacgaaca aacgcagcgt
    17941 gatcgtctgg cttccaacct catacaaatc aagagtgtag ataaccgtgg cgtcggggta
    18001 ggtggatctg ccgtcttcta ttaaaacctt ccaactgtgc ggatcactcc cctctccata
    18061 tagcagttta aaattcttga aatgggaagg gacatcgata acaacgctga tctgcaatgg
    18121 gttctccgta atgatggtgc cgttgttcaa tcccacgaac tcaatggtag gttgagaatc
    18181 gctttcagaa cattgctcat ccggcgtgaa gatgaccgga tcggaaaagc ccatttcttc
    18241 agcccacgct cgcccggcac tggtatcggt gatccatttc cgaccccaat cgtcactgac
    18301 gttcaatgtc cacttctctt cattgaactc gctgcaaaag atcgatgcct ctttgttcgt
    18361 ccaggtatct aaattcactt ccttccacag atcatgatca gcagacagcg gaggttgtga
    18421 gttgttgaag atctcacttt tttcactcgg gcatttctcg gatggtctgg tgccgctgac
    18481 ggaacatatg atttccgtga tgatcgcaga tggctgcggg atggaactgg gcgcgccccc
    18541 ctggtaccgc tgcaccgcat actggatgac atctgcccaa atcggagctg ctcccgtcag
    18601 accggtggtg ttctgcatag gcgtgtaatc agcatttccc acccacacgc ccactgcaat
    18661 atccggggta tatccaaggg tccagttatc gcggaagtca ttggtcgtcc cggtttttac
    18721 cgaggctttg aagggcaatc tgagaatgga attcgcccca aaggcaggag tgcgggcagc
    18781 attatctgcc aggatatcgc tgatgagata ggcatattcg gagccgatga cctgttcggt
    18841 tttcagctcg tgttcataaa ccacatttcc ctgatggtca acgatctgtg cgatggcgaa
    18901 tggctccgcc ctcacgccct gattcgcaaa gatcgcatac gcagaggtca attccagaag
    18961 cgaaacgtca ccgccaccca gagtgagcgc cagtccataa tcggagcggg tgagcgtggt
    19021 aatgcccatc ctttcggcaa atttgatgaa gccttcttct tcaggcgtat tgggatcgtc
    19081 ataaatgccc acaaactgca gggtctttac cgccggaatg ttgtacgagc tgcccagggc
    19141 agtgcgcacc agcacaggac catggtattt gctatcatag ttcaccggga tatatggatc
    19201 gcgcgtatca tttgcatttc ctgaaggtgg gaattcgctt ttcacatccc agatcaaagt
    19261 ggagggagtc cagccttttt cgaacgctgc ggcataggta agcggtttga tggaagaacc
    19321 tggctggcgg ggcgcaactg ccatgttcac ctgtccatct attgtttcgt tgttaaaatc
    19381 tgacgacccg accatagcca gaatctcacc agttcttggt tgcacagcca ccagcgcgcc
    19441 attgccggca ttgttttcaa ccaactgcgc gacctgctcc cgcaccgctt tctgcgcata
    19501 attctgtagg tcgggatcga gcgtcgtata aatattaaag cccgagcggt aaatggtttg
    19561 ggcatcaaac tgttcttcga gcaacgcgcg aatataggtg acccaatggg gaaaaggaat
    19621 attgatctgt ttctgctgaa aaggatattt ttctatttcc agcactgccg ctacagcttc
    19681 ttccatactg acgcacaccg gagcagcgct attgcttact tcgatgcagc tctcttctgc
    19741 gctcaactgg tacatcagat tgaccacatc ttgatgacgc agcaaggtac tttgccggtt
    19801 cgtgaatata tcgtggatag cgggagactg aggcagcccc gccaggaatg atgcctgtgc
    19861 caggttcaat tggtcggcag aagtgctgaa atacgtctca gacgctgcct gaatgccata
    19921 agccaggttg ccataataaa tctcgttcaa gtagagttcc agaatctcat ccttactgta
    19981 gcgccgcgtg atctcggcag ctaagatgat ctcctttgct ttgcgctgta cggtctgctg
    20041 gttacgctct tcaggagaaa gcagcaacgt gcgcgccaat tgctgcgtaa tggtagaagc
    20101 gcccgaaacg atctcaccag aggtgtagtt ctgccacaaa gcgcgtacaa taccgaagaa
    20161 gtcaaagccc gggttattat aaaaatcctt atcctcggtg gcgatggtgg cagccacaat
    20221 gtagggtgac atatcttcca gagaaatata ggtgcgaaaa ccagcattgg gatcaaggat
    20281 ctcgtagatc agattgccgt tgcggtcgta aatgcgcgtg gtctcgaatt gagaagctct
    20341 ggctttcaaa tcttccacgc tgggcagggt ggaggcaatg gcataatatt gcacgagcag
    20401 gaagatagag aagccgacga aaatgatcag gatccccaac aagaaccaca ccacaaccct
    20461 gggaaaacaa cccttcccct ttccagcgcc tggcggtttg cccttggcag cttttcgatc
    20521 ttctctccat ttctcaaagg aagatcgtaa atgagacaaa gcggagcgca agtttttcag
    20581 gatggtagct cccatctctt tgatcgggct agaagctgca ctccttttca ccggaatctg
    20641 gcgggtttca tcttttcgcg ggatctgttt ggtagattcc atggaagagg agccgcgggg
    20701 ctttgaaagg ttctcagtta tttccttttt tttcgactca cgaaactctt ctacgctgta
    20761 gattccaacc ttcaagacct tctcgcgcca cacatcccca gataaaccca tcttgaggca
    20821 caggtgggtc aggaaatctt cgggcttcgg gagctgttcc cacacctgcg gcaggaaagt
    20881 ggctttatga taaccatctt gcaacaccac gccatcttca aggggttgca gcaattcagg
    20941 caggtcggca ggatgttgat aagaaaggga ttgggtggga gtgaggcggg agatttctat
    21001 ctccaccagc tccagttcga attcacgaag cggtggaaaa cggtagtctt cgcgggctgc
    21061 ggcaatggca tgctcacgca catccagcgc aagcggttga taggcttcca aggtgcctat
    21121 gcatcctcgt aactgaccgt cgatagtcag ggtcacaaaa ctggcgccat tttcttgcaa
    21181 gggagcgggc agcgctgcca gatcaatttc tggcaacggc tcgttgcgca ctgccgcctc
    21241 aatggatgtt cgtgcaatct gaagcagcgt ggttctctcg aagtgtgtca atgcaggttt
    21301 tttaatcatt tttactctac ctgccttaaa gtataatttc aaaatggatt atttttaggt
    21361 agagaataag gtttaagatt gtatatgtca aagacatgat gagaaaagat cctgaaaccc
    21421 aacccactct ggaaaaaagc acctcccctt ctctttttaa caccgggcgg gtactgcaaa
    21481 ctgtcatcct ggtggcggcg gtgctggcaa ccctgttcac gctgtggaac ccttataaat
    21541 tattctcaca aaaagaagtt gcccaaagcg cctatcagga agatctggca aacagttcag
    21601 ccgattcctc cacgatcggg attttggtgg ggcactggaa agttgatagc ggtcatgtat
    21661 gcgataacgg cgttgttgaa gccaatatca ccgaagcaat ttctaaccag gtcagcataa
    21721 aattgaacgc ccttgggtat ccagtcaagc tgctggcaga aacggacctt gacctggtca
    21781 attaccgcgg tctggttctg gttgctttat atactggttc gtgtgatgac agcgctgaaa
    21841 acaaaagtgg tttcaccatt gggacaacac tctccaccac agatctgaat tcttccaatg
    21901 cgttggcggt ctgcatgggc gaggtctatc aggcttcgac caagcttgac ttttcctacc
    21961 agatcatttc acccgatcaa ccggcttata cttactttga aagagtcaat cgcaatactc
    22021 ccatgatcta ccttgaaatg ggctccctgc tgcacgaccg atccttgtta atagaaaatt
    22081 ctgacaaagt agccaatggc attgtcaacg gaattcaatg ctacctggat acactggaaa
    22141 aagccgaatg aacgatacaa caccgcacgc gaacaccatc tccgtcatgc tcgagaaagc
    22201 tcaaagcgct ttgaaagccg gagataagaa agaagcccat acctgggcag agaaaatatt
    22261 ggcgacagca cccgagaacg tggatgccca cttgatcctg gcagctatcg gttctcccaa
    22321 ggaaagcctg cgtcacctga cccgcgtcct tgaattggat ccgcagaaca agaaagcgcg
    22381 caacggaatg cgttgggcga tcgcacaggc aaaattggaa aaggaacagg ataagcagac
    22441 cagctcaaaa gaagatacac aaaagatcaa ggtcaaagcc tcctccacag cacaaaaaac
    22501 aagaaagaag aaagcgcaaa aacataccgc tatttgggca atcttactgt tcatcgcgat
    22561 cgtcacaggc ggattgctgg cattcagttg gtatggattg cccctcctga agtccgcttc
    22621 taaggaagtt aaggaaccgc gccccgcagg agtggtcatc aagcccactc tcacccccac
    22681 gccaacctct acccccactc ctacccttac gccaacctcc accctgaccc ctactcccac
    22741 gccaaagccg acagaaaaca ggacatacct cgattatttc gcccattcct gggacattcc
    22801 cgatgaagtg agcggcaccg acaagttctg gatagaggta gacctcagtg agcaaatggt
    22861 ttacacctac cgcggtgaca catttttgca atccttcctg gtctccaccg ggacgaacgc
    22921 ccatccaacc gtaacgggca cattcaagat ctacgcaaaa taccctacct acctgatggt
    22981 tggggatgga tattacctgc cggacgttcc ttactccatg tttttctaca agggttacag
    23041 cctgcatgga acgtactggc acagcaattt tggcaccccc atgagccatg gatgcgtcaa
    23101 catggtaacc agtgacgcaa aatgggtata tgaaaatgcc cccatcggaa catacgtata
    23161 tgtccacaat taacctccag tgaaagcgag catgaagaag agcatcagcc gaagagactt
    23221 cctaaaatat tccgtgcaag gggcgatggg cgggtttttc tacctgcatg cgtttccaaa
    23281 gaccttcacc ctgtttgatg ccgatgccag cgcgccattg atgggaagga ttttcaaaga
    23341 taacaccgtc atctatcaag aaccctcttt ttcagccggg gtgaaatatc acctcacagc
    23401 caatagcgtg ctgcccctgg caggagaagt gatcggtgat cccatccatg cacacagcaa
    23461 actatggtac gaggcgagcg atgacggcta catccctgcc aacagcctgc agccggtgcg
    23521 ccagcacacc aattcaccca aaaccaagat caacagtgcc ggtgagttgg gaacggtgac
    23581 cgtaccgtat accaaggcat ggttagctga cccttcttct gcacctgaaa agtatttgct
    23641 tttcttctat gggtcgaccc actgggttaa aaaggtgtac acagacgcca aaggggatac
    23701 ctattacaag ataatcgaag accgctgggg tgatgcgtat tacgtacagg ctgagcatgt
    23761 agcgatcatg caaagcgagg aactggcagc ctttgcagac gcggtgccgg acgcagaaaa
    23821 gttgctggag gtcaatattg ccgaccagac tgtcagcgca tacgagtatg gaaaaatggt
    23881 cttcttttct ctggcttcta ccggcgtact gaccgaagag gtggaccttt cgacaccgcc
    23941 gggagaatac agtatcaact ataagcgccc ttcccgccat atggtccata ccgataagag
    24001 tggcggcgat gacggcgaac tttacggcgt gccgtgggtc acttatttca ccaacagcgg
    24061 catcgccttc cacggcacct actggcataa cgattttggc gtacagcgca gccatgggtg
    24121 tgtgaacctg cccatacccg ctgcaaaatg ggtctatctg tggagccgac ctgccgtccc
    24181 ttttggacag gaaaaattcg tctccaacac cggaacaaag gtcatcgtaa gctgattcat
    24241 ttttagattt tgttaagaca catcacccga tttcatgatt agaatttccc acatatattt
    24301 atgaggtttt cgaatgagtc agcgagcagt gtttgaagga ttggtgatcg atgaaaatga
    24361 cagaccggtg agcaccgagt atgtgggcac tgaagctacc tacgtagtgg acgatgatgg
    24421 ctttatgcgt catataccca gtgaatatgt tgaccggcag gtgctgaatt ttttaaaaga
    24481 ccagatcctg cagaacaaag atatcttaac cgatcaggcg accaaaatgc tcgggcagga
    24541 ggatatcttt acgcgagcgg tcatagaaaa ccaattggaa gatgtggata aacaaatgga
    24601 cgtgctgttg aacaccgggc tgcccgccag caaccgtgaa tatctgggaa tggtgggatt
    24661 cagggtgcgg atcgacgtgc acggcgatat tctggaaatc gtccagccag cagtaagtga
    24721 tgaagattag catctgccca tcttctgaga gagtaaaatt aaagaaaatg actgtggtga
    24781 aaccatgaca tcagaattca tcattcacgc caatgagctc aatttcgaat atgaggtgat
    24841 ctcgttttcg atgaatacgc ccgtggtcgt ggatttttgg gcggagtggt gccagccatg
    24901 caaaacactg acccccattc tggagcgcac cattctcgag agcggtggtt ctatccgcct
    24961 ggcaaaggtc aatatagatc agaaccccaa tctggcagtg caatatggcg tgcgcagcat
    25021 cccaaccgtg aaagccttcg tgcagggtgc ggtggttgcg gaatttgtag gcagccagcc
    25081 ccctgacagg gtgcgcgcct tcttagaaca gctcacccct cccggaccca ctgacctggc
    25141 gatcgaaaag gcgtccaact tattgatcct cggaaactgg gcagaggccg agactatttt
    25201 ccgcgacgcg ctcagcagca agcccaactc aacttccagc ctgctgggat tatccaagtc
    25261 tttgctggcg cagggcaaag ccgatgaggc actagagctg ctgcgcgaat tccccgccag
    25321 ccgggaatac agccgggcgc aggcactgat gcccctggct gaaagcttgc gcgacttcat
    25381 gcaaaaccga ctccttgatg aaaatgaaaa cgatgcaact tttcgcaact ccctgcggtt
    25441 gataagcaaa ggcaacatgg cggctgccat ggacggaatc ttggggatct tgcgccgcaa
    25501 caagcgctac cagaacggaa aagcgcatca gatggcgcta gcgatcatcg aaatgctcgg
    25561 agaagaaaac cccctcaccg cgcagtatcg ccgcgaactt gccagcgcgc tgttctaaat
    25621 tgcaaatggc atactaatcc agcaccaact ctccccaggt agtcggatcg agaaacgcgt
    25681 gcgtggcaat attcgccacc aaggtttgct ggacaatctc agcgggattg tcattatcag
    25741 atacagagaa ctcgaagccc agacgcaatc ctttctctgg agagatttgc agcaggctcc
    25801 agggaatgcg cgcctcaacc cggtagatac cggctgtgct ggtagaagcg atttcgatct
    25861 ggtcagagat gctgcccttt tttgaaagcg gataccacag ataagcttcc ttcggtccac
    25921 tgatgcttcc tcttccagga gcgatcccta tctgaaaatc gtcctcattc aggctgtcgg
    25981 agtagaaatc tccctgaaga tcggtatcga taagcagctc gatgctgtcg ccgcggaaga
    26041 tctggtcacc actggcattt tgaacgtagg tatcatcttt cactttcaca cctacataga
    26101 gataatcttc atcccatccc aggatgaagg acgcttcaca atcagagtcg ttctcccagg
    26161 cttttagccc atacaccaca taataagccg ggtaggcaat ggtattttgc ttccaatctc
    26221 cccaatcccc atccaggacc ggctttttgg aaagatattt ggcaaccacc cgctgggcgc
    26281 tgcgctcgag gatggctggc gcgcttgggc tgagagcagt cgatgaggat gaactgacgg
    26341 tggaggaggg agagagcgcc tgaccggtca cctctgaggt gggggtggga aacgcagcga
    26401 acatggctgt gagcgtttgg gcaggctggt caaagatgct ggttggttcg gccgctttag
    26461 agaaaggaaa attacaggca accaacgatc ccatcaagag tacgacgaga agaagttttt
    26521 tccgcatata ccttgcactc ctttgatagt taatatagta acacaatcag aaagggtggt
    26581 acccgttcgc acacagccat tccacggaat cataaaaaag atgaggcgat agaaattctt
    26641 tgcaccaata tgcacctgta atccacgggg gtgaagaccc ctggcagaac accatggcaa
    26701 cggtttcagc tctttttcct ctcagtcgcg ttggaatccg ctgctttctt ccccatgatc
    26761 acaacacaca caacaagcgc aaccaacgcc agaacatctt gaagctgtac gccgacatgc
    26821 ttcagatcag catagaccat ccagggccag ccaaccacat gcagaggaag gctcagcaaa
    26881 ggagaaaaga cctcttcttt tcccaaaatg gctttgctta tgcgcccggt gcacccataa
    26941 gtcagaccgg caacagcata tgccaggagc acacggctca tccacacgat gattcttttc
    27001 atgtgtatca cttatcacta ccctttcgtc aattgtgtat ccgtcaccct tttctctttg
    27061 ggtggaaagg tgctgtcata ccgttcaagt tcatcctgat cgatattgaa aaacacatca
    27121 tccagaaaaa gcctgccgga aattccttgc aggctgcccg cagtcagttc ttttaccatg
    27181 aaagcgtcaa aattctctcc ttcggcggtg ctgatggcga acttttgcgc ggcttcaaat
    27241 ccaaagcggt gatagtaggc agggtcgccg aagatgagca ccgccttata gcccatctgc
    27301 ccggcaagct gcagggtgtt cctgatcagc aggctgccga tgccctgccc ctgataagcc
    27361 ggcagcaccc ccaccggacc gaaggtaagc gcctcctgcg tttttccgat gtcggaaacc
    27421 acgctgcatc tcgagtacac gatgtttccc acgacctttc cgtccagctc tgctacgaaa
    27481 tccagtcttg tgatgaaatt gggggaattt cgcagattat gaagaacgaa atgttcatca
    27541 caaccgggtt tgtacaaccg ggtttgtaca cattccaaaa agcttctctg gttagattct
    27601 cgaccgttcg aaaatcgttc tcttcttcaa gacgcaggtc aatgttcaat tgaatcctcc
    27661 acttcgatca tgctcaaaag agtgcttcaa tctttataac catttcgatg ctgtgcagtc
    27721 tatcctcaaa accttcattg aagaatgtga tattccgcaa cggcgatctt ctccgtatcg
    27781 gcatcagatt catcactaag cttccttgtg aatgccatct gcctgtgttc caaaacgacc
    27841 tccaggaaat acaggaagat acccatatcg atgccgttga aataatgaag cttcttttta
    27901 ggcatgatga aagagcgtgc cgaagtatcg cgatatatct tgatatcatg atcgctgctt
    27961 accacctgcc agggctgcga attgccggca ctgggcgcac agcgaaccac atccgtcacc
    28021 ccggcatcaa attctccctg ccatatcata ctgctgtctt tgcgtttgca ctccgacaga
    28081 tcttttctga aatcttccgg acggctcttc cctatagcga gcatgatcac atactccaga
    28141 ccgtcgttct gcaatttctc cggtttcgcc atcccaaccc aacacgcacc gatatcgaga
    28201 gaagcgaaga acagatccat ctgctcgagc atatatccgg cattcaaaag gtagcgcggt
    28261 ttctgctcgc tgtacagcag caggcagtat tccccgaacc tggcgctggt ctcttccctc
    28321 tttacgatct tgaagctgac ccgtatatcc ttctccagcg ggaccagggc tttgatctgc
    28381 tggcggatca attccatttc ttcttcggaa atggacagtg catcatcgaa ctttctgatc
    28441 gattttcttt taaagatcat gtcatagaat ttatccatca tttcattctc ccttgctctt
    28501 catatacgag aaaaaagcaa attagttcgc ctgatgcagt cacaaaactg ttagccgtca
    28561 aaggaattta tatatttcct acctatatct tgaacttcag cccaaaaatt ttttcaatcc
    28621 tatcgcgttt gttctctcct aattctttgc gcagggtaat gatattaaca gagtcatatc
    28681 caagcttata gtaaagacgc agcgcatttt catttctggg taccacgtcc agacacaccg
    28741 ctgtatagtc cgggtgatcc ttcacaatct cctctgcttt gcgaatcgca tgagaagcaa
    28801 tgccctgtga gcggtattct tcatccacat agatatcttc tatccatgcc acctgcggac
    28861 ccttgtacca aagatgcaaa aacccaatcc gtttcccctg aaatacgatt atatagaatt
    28921 catgagcttc agcctgccat tcgcgcaagg tatcccgtgc ttctgtaatg ctttcttcgg
    28981 cttccttttt ttcaatattc agtaaactgc tatgatagcc gaaaaaatcc gcaattcttt
    29041 taaccgtctc ctcttcatat gtatccgagt agggtatcag ctcaaccatc ttctgcctct
    29101 ctgagtgggg tattttcatt cttttcctgc agcacgcagc aacgcaaccg tttcaaaatg
    29161 gtaggtttgc gggaacatat cgaagagggc gatcttttcg accccaaagt cgtttccacc
    29221 aagccgctga acatcccttg ccagtgtggc aggatcacag gaaacataga tcagcgtggt
    29281 cacaccactg tgtaccagcg catcgatcac atgcgcatgc aagccggcgc gcggcgggtc
    29341 gcacagcacc aggtctgcct tgaacggcaa acccggcatg atcgtttcgg cagagccttc
    29401 atataacgac acgttatcga aggcatccag attgaccgca aaattccggc aggcacgcgg
    29461 gtcgctctct atggcaacca gttcatcgat cagcggagcg ataaagcgag agaacagccc
    29521 caccccgcaa tacaggtcga gcactttttt aggggtcagt tgctgcacta tggttgtaat
    29581 gtatgaaacc atgctttccg ccaccccgtc gttcacctgg aagaacgaac cagcggaaag
    29641 ctgaaaaggc acatcccgca cccacatggt aagagcatcc tgcccgctca aaacgatagt
    29701 ttcgccacca ctgcaatgca ccatgttcac cggcgcactc acctcgatct ccggtatgct
    29761 gccgggcgcc ccctccaggg taaccagcag ctcttccgca cagttctggc gcagttccac
    29821 ccgtgtaacc tcactgcccg gttccaatat caacgatgac catgcgcttt ggatcgtctg
    29881 cccgggcagc aggcagcggg tgacctcaaa aacggcgttg gaatcgcgtt tcatgaaacc
    29941 catcttaccg tctggcaggg ggtggaattc cagcacattg cggtagccgt aagccaggtc
    30001 agatgggaac accgtgattt ccccgacgtt atccagatga gcgagacggg cgagctgctc
    30061 gcgcaggatg tcttgcttat atcgcaactg gtgatgatac tggatatgct ggtactggca
    30121 gccgccgcat accccgaaat gggggcaggg cgcagacacc ctttcggcag agggagcgag
    30181 cagttcaatg gcctcggcgc gggcatattt ctgtttgtca tccacaactc gcactgaaac
    30241 cttttcacca gggatgacag cgggaataaa cactaccctg ccatccacaa gcctggctaa
    30301 cccatctcct ccatatgcca tccgttcaac gaccacatcg tgcattgtca gtgcattcat
    30361 acagaaaatc atacccgaag aaccgtcatc catacgaagg aaatccaggc attttcacaa
    30421 attccgtcga caaaaattac cgttgaagaa catccaatga acatcccttt ggggcttgca
    30481 aaaaaatcca tcttgtgctt aaaaaccagt tataataacg aaatgcgaaa acaaactatt
    30541 tatacaatca caagcacctt tctttttgct gcattggtgc tgagcgcttg cggcgggaat
    30601 gccgaagcgg aagccacacc gaccatggat accgagagtg tcatcgccac ctccatcgca
    30661 cagactatgc aggcaatatc cgaaacgcaa acagcccagg tgacgcctac cctgctcgcc
    30721 accgccactt cggcagtggc tcccactatc acccctgcca caggcggctc accgactgcc
    30781 accgttcaga gcgtgtcgct gcccaccaac tgcctgatcg ccgggctggt gagcgagacc
    30841 atccccgacg gcacggtcat cgccagaggc gccagcttca ccaaaacctg gtccatccgc
    30901 aacggcggca cctgcacctg gaccaccagc tacaagatgg tcttcgaaag cggagagctg
    30961 ctgggagcag ccagcgaaag cgtagccctg acccagaacg tttcaccggg catgatgacc
    31021 tcggtgagca tcaagatgac cgcccccagc gtggatggca cctacatcgg ctattggaac
    31081 ctgctaaccg acgcaggcgt gctggttggt agattctcag tgaatattta tgttggcacc
    31141 gccaccgcag ctcccttcgc ggtaaccagc gttacatacc caaaaagctt tagcaactta
    31201 acatgtgatg gcagtacttt aaattcaatt ccagtttaca ttactactga tgcggcagga
    31261 acagtgactt tcaccctcac agatctcaac ggggtcagcc ctacaggagc aggtgataaa
    31321 gattttggca gtgctggtac tcaaacgatt tactttaaca tgaagtttcc cagtaaagct
    31381 tctggtcaag gcgtagacgt ggatatttct gtgtacatag ataatcccaa tcaccagaca
    31441 ttcttgttga aagattcaga cggagtatgc aaataacaca aaaaagcaac ctcttgaagg
    31501 ttgctttttt tatccgtact ttcgaacgcg cgtttctcac attttctgca tgatattcat
    31561 gaagcgggta tggctgatgg gatgatcggc gatcaccttt ccctcgtata caatgcaaaa
    31621 ggatccaaac ggagcgggaa cctgttgcgc agtttgtgca tcttcaatct cgatcaagcg
    31681 agtcgggatg tgcatttgct cagccgacgc cagaatcgca tccacatttt tcaccgaata
    31741 cgggcactgg gctgaacgaa aaacggtcaa gcctttcgca taagctgcca gtctgtccgc
    31801 catatccaat ttgaaagcag ggtcgggttc ttctgcattg aacttcaaca cggaaagttc
    31861 aaagtcggga gccgcctggt ccacaacgtg aaatccattt ttaagaaaga tgggtttatc
    31921 tgccatgaag gaaccctgac gggtcaccac tgtcacaccg gacctaccct gggcgcacgc
    31981 atcctgaatg cactcctgca acaagagcgt tgctaaccct cttctcttat attcctttcg
    32041 aaaaccgaca aaaagacagt ggatgaacag atacttttgg gcattcaccg gacggtgagc
    32101 gtactcgccg ggaatgtatt cgatcattcc ctgggttccc ccaccttcta tggtgatcac
    32161 cttgatggta agtcccttgg gttggaaagc ttccatccat tggatcttgc gttgcagttc
    32221 agcatgtttt ttcgcatctt tatacccaca cagaccatac tgaccggcat tttcaggggt
    32281 caacgtaacg atctcaatac cgtccatttc cacctcaata acaaaatatt aatttataga
    32341 ctttgaataa tatgaaagtc caatctcatt ttaacaaatc ttttcaccca gagtatcatt
    32401 agaaattgat cgttttaaat taagagcgga gagggtggga ttcgaaccca cggtaccttg
    32461 cggtacacac gctctccagg cgtgcgcgtt aggccagact acgcaacctc tccttaaaat
    32521 aaaaacagca tgcaattgtg ccgcgaaatt ataacatgag ttgttttaaa accagggaac
    32581 aaacacccgg tttcaaaaaa tggacagaga aaagggtacc ttatggtaag catcaggaaa
    32641 atgaaggtca gcgattacga tcaggcgcta gcgctgtgga agcgcactcc cggcatgggg
    32701 ttgagcgcag cggatgaaaa ggatgaggtc cgcaaatttc tgaaaaagaa caacgcgctc
    32761 tgcttcgtcg ccttcgctgg gagagagctg gtcggaacga tcttgtgcgg cgaggacggc
    32821 aggcgcggct atctgtacca tctggcggtg gatgcgaatt gccgcaagac cggcattgga
    32881 aagagcctgg tgcagaaaag cctgcaggcg ctacagaaaa gaggcattca aaagtgccat
    32941 ctctttgtga tcgcggagaa cgccccaggg atcgcttttt ggaaacacac cggttggcaa
    33001 ttgcgcgatg acatcgaggt catgtcgatt gacctttagt ggtatagttg tctggtttac
    33061 gcggcgggaa aaaaccttta ttttaaagca gattccccac cacccataaa gaccatggag
    33121 ggtcaattct ctctgcgcga ccactcttgc gtatcctgag cgcccctctt ctgatctttg
    33181 tcatgatgtc gcgcgaggcc agcagtttgc ccgcctgcgg tattccgttt tgaacccgca
    33241 agcaaacagg attcagtgtt aagatatgga aagtggagtt cagcatgaaa aatcaattca
    33301 aacaagaaac atggatgggg gtgttgtgcg gatgcgtgtt cgccgcggta gcctgggggt
    33361 atgatgggta cttgctttcg aaagcgcacg ccgccctgcc ctggttgaaa ctggcgctgg
    33421 ggctgccacc ctgcgcagcc gttggcggat tggcgggttg gttgaccatg aagatgagca
    33481 atatgttcac gcgcatggct ctatgggtcg cggttgcgat cttcttcagt tatttgacca
    33541 gcctgatccc cttcacgctc tcgcccatca tctaccgttg gtctttccct gccgtgagcg
    33601 agcgcatcat ctataacgca tcggttgcca tcagcgcgcg gcgtttcatc accattgtca
    33661 tgaccattgt cttcctcttc atcgccgggt tgatcttcga aaatgtgaac gattcggttc
    33721 gcaacgctcg tggcatagtt ggggtagtct tttcagttat tttcctgctg gcattcttcg
    33781 caggcgccgg atataccgcc gacagcaatt tcaacgtcga cctgcgcacg cccattctgg
    33841 ctctggatga gaaattagac tatgtggcga gcgtgaacgc cgccacgttg agcgatgtgg
    33901 aagcgcgggc gatcaggcgc ttcacgaaac tggatgttga ccttcaaagc ccgcgccgca
    33961 cggtgatcgc cacttttgat gaatacatat cgcagatgat agtggtggtg gattttgacg
    34021 ggacgctggc agagtgttcc gtgatgaacg ggcgcgcttc atcgtgcaaa atggtggaat
    34081 aaagaggtat ggcggattgg gaggttggaa ttacgtaatg actcaataga aaaagtaact
    34141 ttgatagtaa cgttgactca gatagaa
//
LOCUS       DAOSDS010000014        33309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_659_length_33309_cov_4.549768, whole genome shotgun sequence.
ACCESSION   DAOSDS010000014 DAOSDS010000000
VERSION     DAOSDS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 33309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33309
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_659_length_33309_cov_4.549768"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /gene="deoA"
                     /locus_tag="PLL88_03125"
     CDS             <1..531
                     /gene="deoA"
                     /locus_tag="PLL88_03125"
                     /inference="COORDINATES: protein motif:HMM:NF019448.2"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HOJ00586.1"
                     /translation="ETLHGKGPADFREHCFTVSAHMLLLGEKAQTLDEAYAMAEKAIV
                     DGKAWDKFVQLVKAQNGDLSFIEHPEKLPCGSIREEIKAENDGFLAMVNAQSVGEAAV
                     LLGAGREKKGDPIDLGVGITVHYKVGTLVKKGDVLFTIYANDRERLQVAKLKLKESVN
                     ITDEECAELPLFYGVV"
     gene            862..4026
                     /gene="ileS"
                     /locus_tag="PLL88_03130"
     CDS             862..4026
                     /gene="ileS"
                     /locus_tag="PLL88_03130"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOJ00587.1"
                     /translation="MFKPVSSKLEIKAQEESILEFWKQQEIFKQTLKKTEGSPKYVIY
                     EGPPTANGMPGVHHILARVFKDIFPRYKTMQGYHVDRRGGWDTHGLPVEIEVEKKLGF
                     TNKQQIEEYGIEKFNRLCRESAFSYIQEWERLTDRIAYWVDLSQAYVTYKNEYIESVW
                     WILKTLWEKDLLYQGFKVVPYCTRCGTPLSDHEVSQEYHDVTESAIFVRMPLLDEEDT
                     SLLVWTTTPWTLPGNVAVAAGRDITYVKVERDVPNGEEHAKEKLILAEALVEKVFGEE
                     KVAILDRFKGKQLKGKRYQPLFTFLPVEKPAHYVVLGDFVTTDDGSGLVHIAPAFGAD
                     DMNVANQEDLPVLMTVKPDGTFIREVDQWAGKYVKDADPLIIDHLTARGLLFKVEKHT
                     HSYPFCWRCGTPLLYYARSTWYVRTSQVKDRLVALNNEINWYPEHIKNGRFGNWLENN
                     IDWALGRERFWGTPLPVWQCEDCGMQTCVGSVEELSKLVGHDLTGLELHRPYVDEVVF
                     DCPECHGKMQRVPELIDVWFDSGSMPIAQWHYPFENVEEFKQQFPADYICEAVDQTRG
                     WFYSLHAISTLVFDQKCFKNVVCLGLILDENGQKMSKSRKNVVDPWEVIEQNGADATR
                     WYLYTASPPGQERRFSTSLVSEVIRTFMLTLWNTYSFFVTYANLDQWQPDTQKKDIEY
                     SPLDHWIRSELHALVRDVTTAFENYDVINATRPIEAFVDHLSNWYLRRSRRRFWKSES
                     DGDKYAAYATLYEALVTVSKLIAPSMPYMAENLYQNLVRSVDSNAAVSVHLTAWPRCD
                     EKVVDAALNHEMEVVMKLVSLGHAARNKSGIKVRQPLAEAAFAQSAADDGGIVERYAD
                     LLEDELNVKKVRLLSAANEAVAYELLPLPKQLGQRFKGVFPKVKEAILKLDAEKIAEA
                     VSKGESFPVVVDGKEISINPDEVEIHIQAKEGFEVASEGAYLAALVTTMNDDLIYEGL
                     VREFIRRIQESRKQASFEIADRIKLSFSASEKLAKAIEEYRDYVMNETLAMQMDAKNH
                     PATLPNASDEFDGEQVTIWLEKMR"
     gene            complement(4077..4418)
                     /locus_tag="PLL88_03135"
     CDS             complement(4077..4418)
                     /locus_tag="PLL88_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOJ00588.1"
                     /translation="MSHPSWLENIPIAITVSDAEGKIIEMNQKAADTFASSGGKDLIG
                     KNLFDCHPSKAQEKIRQLSQGTTANVYTIEKNGIKKLIYQIPYFQNGKFAGLVEFSFI
                     TPDVIPHFIRD"
     gene            4568..5059
                     /gene="lspA"
                     /locus_tag="PLL88_03140"
     CDS             4568..5059
                     /gene="lspA"
                     /locus_tag="PLL88_03140"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOJ00589.1"
                     /translation="MKKNIKAYAFLFVLAGLIIALDQFTKNLVRTNLAYGEVWSPWEW
                     LMPYARVVHWYNTGVAFGLFQGMGLVFTFLPLIVVVLIFIFYSRLAGDNWWMRTAIGL
                     EFGGAIGNLIDRITIGHVTDFISVGNFPVFNVADSAITVGVCVLLIVLWKEEKKNSIK
                     PAQ"
     gene            5061..5984
                     /locus_tag="PLL88_03145"
     CDS             5061..5984
                     /locus_tag="PLL88_03145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ00590.1"
                     /translation="MSVNGDIREFVFSGDTPVRLDKYLAEVLPSQSRSRLQALIKSGF
                     VRINGSIVNKPSTLITAPCKIEIEIPPAQPGELVPEDIPLDILYEDKNVIIVNKPAGM
                     VVHPAHGHPTGTLVQALLAHTPFLQGIGGVQRPGIVHRLDRDTSGVILVAKNEKTHRW
                     LQDQFKSRKVVKTYYALVDGHPPTPTGRIEVAIQRDPRNRKKMAVAYGETGRVAVSEY
                     RMLHSYKNHALLEVRIFTGRTHQIRVHMAYLGCPVVADTVYGHKHPSLPLARQFLHAR
                     SISIILPDQQEQREFVAEMPADLQQILNNLI"
     gene            5995..7044
                     /gene="ltaE"
                     /locus_tag="PLL88_03150"
     CDS             5995..7044
                     /gene="ltaE"
                     /locus_tag="PLL88_03150"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: protein motif:HMM:NF007825.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOJ00591.1"
                     /translation="MEQKMTYIDLRSDTVTIPTPAMRIAMENAVVGDDVYGEDPTVNE
                     LEKKAAALLGKESALFLSSGTMGNLLAILVACKRGEEVIMGQAGHTFLHEVGGISALG
                     GVFSNTLPNQPDGTLHLEMVREAIRGDDIHEPRTKMIVIENTQNQCGGVPITFDYMKR
                     LGNLAKEHNLFLHVDGARIFNAAVALHSSARELTAMADSITFCLSKGLCAPVGSVLCG
                     SGGFIQEARRYRKMLGGGMRQVGILAAAGIVALDTMIDRLAEDHARALQLAENLAEIP
                     GFSLPKGLPRSNMVFLGVDRTVFPDLQMLQEKIKKKGILFGFSGSDEIRLVTHYWITD
                     ESIRSVIECWQNLQA"
     gene            7098..7934
                     /locus_tag="PLL88_03155"
     CDS             7098..7934
                     /locus_tag="PLL88_03155"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOJ00592.1"
                     /translation="MTEFITLNQINSAVTVIRKRAKQQPKVGLILGSGLGDLADSVIA
                     PDCVDYKDIPGWPVSTIQGHKGRLVIGELAEIPVLIMQGRAHYYEGYSISQIGFPIRV
                     MRKLGIETLIVTNAAGAINPAFKPGDVMVITDHISLMAMGGENPLRGPNLDEFGPRFP
                     DMSRCYSTDLINKAKSVYATNNVDFHSGIYVCLAGPSFETPADLRFLKLIGADAVGMS
                     TVPEVIVAAHSGMQVLGFSGISNKANLDGSTITTHEEVLQAGKEIAPKLEKIIRGVLA
                     TL"
     gene            7965..8648
                     /locus_tag="PLL88_03160"
     CDS             7965..8648
                     /locus_tag="PLL88_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00593.1"
                     /translation="MTGLLRFLKEFEGGIYLLLLVLALVMGSRLVSANQLKKKTPFGL
                     EKELVQKRINATVAYLAIIGILFIGQILLVSVASVRYPGLYTITTPTSGIIPTPTDFF
                     QAMLRMEETPEGLEQTQTASALTGCIPQQIEWISPKSGDEISGSVSLSGTINVPNMGF
                     YKYEYRQKGREDWIPIAAGNKVIVASELGGSWNTEQLEPGLYELRIVVSDNQNNLFEP
                     CVIEVKVVL"
     gene            8664..9203
                     /locus_tag="PLL88_03165"
     CDS             8664..9203
                     /locus_tag="PLL88_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ00594.1"
                     /translation="MAEVIIRTVVSPDFSILSSFKHSLETQTVWQMDQEAVDDSIAVS
                     FRELKLPRLMKLSYPRSAQSLFDRWKDLSIILVGCIDNVPIGYISCSSIQATSNVWIK
                     DLVVHERWRRQGVATSLVKAIGVWGEERGLKRITLEMSSKNYPAICLAKKLGFEFCGY
                     NDYYYENNDIAVFFARLIR"
     gene            9212..11164
                     /locus_tag="PLL88_03170"
     CDS             9212..11164
                     /locus_tag="PLL88_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00595.1"
                     /translation="MANSVENVNNWVLIFKTVSDVLTAGLAITTFSLFLYSLTFKIRD
                     QLINIYSGILFTLILIFGADAFGTITNEKIALLNILKIHWLGLIAIPTLYFLFSDALL
                     ALTGKPSHGKRRIFGFMMIATSCFFCVLLLADGLLGELIVDQKPAPYFLRTKAMDLYL
                     AFFFSSILLSLYNFFRAIRRTNSPTSKRRMIYLVVGAIGLFLGLFPYLIFESSMGVLN
                     SLTFWILSCILYLGSWILLSTMTYSISFFGLSVPDREIKSRLFHWIFRGPLTASVTLG
                     LTTLIRRIGYLLDKDLLMFEILAMVACIMLLEHAYTILFPKMEKILFRGKDLQEMERI
                     RSLEARLFTRNDLNQFAEIILAALCDRMRASNAVLFLKPNGGYENSVEVGNPARIIEK
                     VKKANYQFNEDWEKKTIFYWNGIVIIPIKDGDERLNNSLISGFIGLDKIKRTDLDPDQ
                     VRAVNLLLSRLHTAIVDYQQQQKVLYSLELLEPQMVEIQNLLAAGRFTTIAMPINLED
                     TEVLNRWAKDALSHLWGGPKLSGNPLMQLAIVQQKAHESGETLTKALRELLQGAIASL
                     KPEGERQYTNDWLLYNILDFKFNGNMKMRDIAKRLALSEADLYRKQRIAIDGVVKKII
                     DMETTLLKNNQKIGGKKNDDEKKRRT"
     gene            11136..12077
                     /locus_tag="PLL88_03175"
     CDS             11136..12077
                     /locus_tag="PLL88_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOJ00596.1"
                     /translation="MTMKKREEPEDTAPVLDESWWNSLLDAANQYQDHEKTGGDHHLE
                     NENIEIDWKTMEEYFRNDEVFSVQVIGYNKGGLLVRGENFKGFIPNSHIKEVSKIQSA
                     SAKFHALKQCVGKDVQVKVIECDQKMGKLILSERAAENAPGKRLDVFEDLSMGSVVEG
                     EVTNITKFGIFLDLGGIEGLVHVSEISWGKVNHPADIVKIGDRIKASVISVDREKMRI
                     ALSMKRLLPNPWVEFNEQYMVGDVITVTINKVVGYGVFAGIEKGIEGLVHITDIQSKD
                     ATLTINDLKVGEKIIVTIKAIDVERRRLSMGFISKFE"
     gene            12096..14195
                     /locus_tag="PLL88_03180"
     CDS             12096..14195
                     /locus_tag="PLL88_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOJ00597.1"
                     /translation="MKSKSNNVVKVLEYFHRFLKDIIGILLISIALISMLGFFHLSQG
                     KVLNRLVEIIEQGFGWGSIFFLFSLLYIGVMVLFRRVESFPDLNLKRLLYIEVFFFST
                     LGFMAIFGGNSLVRATDGKDGGVIGWGIAKIVSSVLPLPVCSILLSLVALLSFLVATG
                     LLRKIVSKIQTRAVPEHKIEKPEGSLPPDLQLNHQPSTGTRTSSSIPNMENAQKNLPP
                     LSLLVEQKSHATDEGYIKQNAQVIEQTFEEFGIPANVVGYRIGPTVIQYALEPGYIDK
                     TSEDGEVSKRKIRVSQIASLQRDLALALGAERLRIEAPIPGYSFVGIEVPNKYSNMVR
                     LRSVLNSDVFQKLNSPLRVALGLDVSGNPIVADLSKMPHLLIAGTTGSGKSVCISSLI
                     SSLVMCNKPEDLRLAILDPKWVELSIFNGLPHLLGEIETKTNRMLAVLEWASSEMERR
                     YKIFEEVNARDITVYNSKAGRRGFAPLPRIVILIDELASLMMSSPEQTEAHLVHLAQM
                     ARATGIHLVIAMQRPSTDILTGIIKANFPARIAFMVASSVDSRVILDMNGAETLLGKG
                     DMLFLDPDNGAPKRVQGVMVDDMEVKNIVEYWNSIYPPGQNGEAEAPWEKFIVNQGED
                     GDELIGRAIELLKGEGRASASLLQRKLRIGYPRAARLMDELEERGIVGPPEAGGRDRE
                     VILDDDRPYGDEEESYD"
     gene            14258..14854
                     /locus_tag="PLL88_03185"
     CDS             14258..14854
                     /locus_tag="PLL88_03185"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOJ00598.1"
                     /translation="MTFTDRLRIIFQRFLDSVGHTLVHFGISANAITLLSLAGSVIAS
                     VFIARGNLLAGGIILACTGPLDAIDGAVARAGGKITSFGAFFDSVSDRYSELMIFGGL
                     AWFFLNRLDKMGMILTFLAAAGSVLVSYTRARAQGVGIDVKAGFMTRVERYVVLVPGI
                     IFRIPKVSLWIIAILANLTAIQRIWIVWNQTKNTEKEK"
     gene            14858..15703
                     /gene="lgt"
                     /locus_tag="PLL88_03190"
     CDS             14858..15703
                     /gene="lgt"
                     /locus_tag="PLL88_03190"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOJ00599.1"
                     /translation="MSDGFYIGPIYIYYYGVIIMVGALAALWLAIKEAKYRKLDPEIV
                     WDVVPWLLIVGIVGARLWHVLTPSKSMGAGIEYYFSNPIEILKIRKGGLGIPGAILGG
                     ILALAIYCRKKKLNFFTWADVIVPGLALAQAIGRWGNFINQELYGAPTDLPWAIHIDE
                     ANRFPEYAQYSTYHPLFFYESLWNLLNMYLLIVLARQYKEKLIPGDLLFTYLIVYPFG
                     RFMLEFLRLDTSTIGGININQITMLVVMIFAIVVLIIRHRKPQEVTQEQPVNEGQVES
                     EEEKD"
     gene            complement(16019..16489)
                     /locus_tag="PLL88_03195"
     CDS             complement(16019..16489)
                     /locus_tag="PLL88_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00600.1"
                     /translation="MNIEKTVKKSTMIMLVSLIIGIVLLATGILLELMDIKLLPNNKA
                     IVALSFTPLVIAVISYVKLRTIKKNPQKMKNIIISENDERLVALKNQADANAFKILQG
                     ILFFSYLGYTFMVPEDVFEAVGWWLITFALLASFVLQGILSAKATDQHDAKSDD"
     gene            complement(16493..16672)
                     /locus_tag="PLL88_03200"
     CDS             complement(16493..16672)
                     /locus_tag="PLL88_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ00601.1"
                     /translation="MKIARVESDISQEHLAEMVGVTRQTINLIESGKYNPSLKLCIEI
                     CKALNKTLNDLFWEE"
     gene            complement(17151..17636)
                     /locus_tag="PLL88_03205"
     CDS             complement(17151..17636)
                     /locus_tag="PLL88_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOJ00602.1"
                     /translation="MLNANFEPINVCNTRRAVGLIVLDRAILVLNGRGIIRTVSRDFP
                     KPSIIRLQYMVHCPRPQVHLTRKEVFRRDNYTCQYCGKTSDELTIDHVIPKHLGGSQV
                     WENIVTACPRCNHLKGGRTLEMTNMRLLREPKAPPSSAEYIFGKHVLQNIDWEPFLRG
                     W"
     gene            17772..18983
                     /gene="tyrS"
                     /locus_tag="PLL88_03210"
     CDS             17772..18983
                     /gene="tyrS"
                     /locus_tag="PLL88_03210"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOJ00603.1"
                     /translation="MNIDEQVALLMQGTEYGDADIQKNMASELKERLIQAEKDKRPLR
                     IYCGYDPTKADLHLGHTVTMRKLRQFQELGHHIIFLIGDFTALIGDPSDKDVTRPILT
                     RQQVEENARTYAEQAFRILDRDKTEIRYNSEWLSKLSFADLIKIASNFTVQQFLSREK
                     FKLRWEKGDPIYVHETFYAFMQGYDAYMLDTDVQVGGTDQFFNIMMAGRKVMSAMGKQ
                     PNIAITVEILPGTDGEVKMSKSLGNHIPISTTADDMYGKIMSIPDKAMPRYFRLVTRW
                     TPLEVETLEKQIKDGKVHPRDAKMKLAFEVTDAFYGTEQATQAQNDFVQKFQKKEMPV
                     DMPDFPITNGQSLLEVIVAAQIVTSKSQAKRLFEQGGVSLDGETVKDWAMLAQPGVLQ
                     VGKRNFLKLVG"
     gene            complement(18980..21577)
                     /locus_tag="PLL88_03215"
     CDS             complement(18980..21577)
                     /locus_tag="PLL88_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOJ00604.1"
                     /translation="MQNQKQLMRLAALFLLVFAAVLTLSPVAKYRSWQVDLRWFHWIG
                     LTLVSLGFFFLQHTLEKCGRNVDPFLLPIIYLFSGWGLLTIFRLGTFYGLRQTLWILA
                     GLLLTWFLVTRKQSVMQLLQKYNLVGLLLGLGLLTATIFWGIFPGGDGPTLWLRILGF
                     NIQPSEFLKLFFIIFLSAYFAKEKDKQRTLKSILPTLFVFLVICGTLVYQNDFGTALI
                     FVIIYATYLFFYTGQKRVFGSIVLAFLLFGVLGYQFFDIFSTRISAWLTAWQDPSGGS
                     YQVIQSILSIAAGGVLGSGPGLGFPTVVPLAHSDFIFAAIAEETGLIGTSCFMICLAL
                     MLERGYKMARNAINPFQSYIAAGVTTYLITQSILIIGGNIRLLPITGVTLPFVSYGGS
                     SYISAFLAISMLFCMDTSQEENAGRSVQRELIKKPLIVLGTLFMIAIALVEGVLFWWS
                     YVKADNLQYRSDNPRLIYADQFVERGTIYDRDYFPLAVTKGKVGSFYRYYPYPQLSTT
                     VGFRHIRYGHAGIEEYLNAYLRGYQGYPQSTIWFTHLVYDQPPEGIDVRLTLDLDIQR
                     KLDELMTGYQGAAIVLDIASGEILASSSQPGFDANELDENWYAWNQDGGAPFLNRVMQ
                     GSYPAGSILIPFILNEEELQGLISSSLLSLQSENSQSPCLLALQGEAAINDAMQNGCQ
                     AALFSLVGNLSGEQLRQRLAGFGFFTPPAIGLPLSATYFTENLAVPVAYVAGSDQIRF
                     SPLMVARAAALVSNAGESVDLSLILDLNNQSLQTGENLQEIDAASPSRTQIDFLMDSF
                     FKKCGNSWEFSGFSEDANGIYQWVMQGTVNGTQNKRTVVVIVLENASAAQALSISNAI
                     YDFMQQMDN"
     gene            complement(21622..22476)
                     /gene="mtnP"
                     /locus_tag="PLL88_03220"
     CDS             complement(21622..22476)
                     /gene="mtnP"
                     /locus_tag="PLL88_03220"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HOJ00605.1"
                     /translation="MNTTPFLAVIGGSGIYQFPALKNVKTIEVDTPFGKPSSPIVMGI
                     LHEKPVAFLARHGFGHVLTPTEVNYRANIFALKKIGAKRIVSISACGSLREDYAPSHF
                     VIPNQLFDFTKRRTYSFFGDGMVAHIGSADPYCSGLRTNVLDAVRQTGSVVHDSGVYI
                     TIEGPRFSTKAESNLFREWGMSIIGMTACPEAFLAREAGMCYVTMAHVTDYDVWHVSE
                     KPVTVEMVLQVSKNNTSNAEKAILNLMDILPPVSHCDCWDALKNALLTEQNRIPESTQ
                     ENLKILFE"
     gene            complement(22473..22640)
                     /locus_tag="PLL88_03225"
     CDS             complement(22473..22640)
                     /locus_tag="PLL88_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00606.1"
                     /translation="MEFSGVLVLFLRGLMAVGLVSFTVFALSILWKDMRHEKDTKKKP
                     RSSLKQKENKQ"
     gene            22717..23124
                     /locus_tag="PLL88_03230"
     CDS             22717..23124
                     /locus_tag="PLL88_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HOJ00607.1"
                     /translation="MKQKQGFKEIEHTADVAMHVWAETLPALFHQAALGLYALAEIEM
                     QEAPVVSRSLCLEAQDVETLLVNFLSELLFQLDRGAVLQVQLLEVDGNTLTGIFMEKK
                     ITQIHREIKAVTYHRLHVVKENSIFRTDIVLDV"
     gene            23133..24584
                     /locus_tag="PLL88_03235"
     CDS             23133..24584
                     /locus_tag="PLL88_03235"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HOJ00608.1"
                     /translation="MVKLNDVRRISDFEWVIPKSLRSGMKTDVRIIASRAILEKSLND
                     LALEQAINAAFLPGLVGEVLVMPDVHQGYGFPIGGVAATDAEKGVISPGAIGYDINCG
                     VRLLASQVEFQEARPLINELLQGLYDAVPSGLGRGGLPRLQKTQLEKLMHEGAGWAKQ
                     VGFAEDSDLELTEDQGKMNGADPSKVSARAVERGAEQVGSLGAGNHFIEVSMVEDVYD
                     PESARVMGLEKSCLCLMIHSGSRGLGHQVCSDYVQEFQKAQRQYAYNLPDRELACAPL
                     LSKEGQAYLAAMRCAANYAFCNRQVMSHLARQTFERILAARCKRPYLRMVYDLAHNIG
                     KIEEHWVEKEKMQVCVHRKGATRAFGPGVPEVPIRYRQIGQPVLIPGSMGTFSWVLTG
                     KSEAMLKTFGSSCHGAGRVFSRKGARKLMRGSQLIQDLHRRGIAVMAGSLAGLVEEAP
                     EVYKDVDEVVESVVQAGIVKKVVKLRPLAVIKG"
     gene            24649..24873
                     /gene="secG"
                     /locus_tag="PLL88_03240"
     CDS             24649..24873
                     /gene="secG"
                     /locus_tag="PLL88_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOJ00609.1"
                     /translation="MERYFQIALMITSVALIASIVVQSKGVGLGGLTGADLGSNITTR
                     RGLEKTMFKITIVLAVIFVALVLGTIAVAK"
     gene            24951..26618
                     /locus_tag="PLL88_03245"
     CDS             24951..26618
                     /locus_tag="PLL88_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ00610.1"
                     /translation="MKNIRWQLIIILVTGFVIGILLISQQTGFSITRTVSNRGGTYTE
                     ALIGNLQRLNPVLDYYNSVDRDVDSLIFSSLISYDENGQPQPELATNWGISYDGLSYN
                     FDLNPDATWHDGTPVTSADIAFTIDLMRNADSVLPEDLKAFWNNVQVIPLGDQAIQFR
                     LTEAFTPFMDYLSFGVLPQHLLGDLSYQEILNSSFNLEPVGSGPYQFERLITENGLIK
                     GVVLSANSAYFGDEPLISQIVFLYYADAVSAYAAYTEGTVQGISQVTRDILPDVLSNP
                     DISIYSTIEPQVSMILLNLNNNKVRFLQDENVRKALLHSIDRRRMIDSVLLGQGVISN
                     IPLIPSNWAYYDGIQNYAYDTQKAAQYFEEAGFTLNSSAENAVLEDEDGAALSIQLAH
                     PDDDTHTAIAQTIQSDWQKMGVQVELVAVPYDVLILDYLEPLTYQAALVDLNFERSPD
                     PDPYPFWDKSQQSNGQNYSQWENTTVSEYLEEARVSTDQEERAKLYRNFQVVFQDELP
                     ALPLYYPVCNYAVDEGVSGIRLGSFYDTPDRFFSILNWYLVAKVNNQ"
     gene            26634..27143
                     /locus_tag="PLL88_03250"
     CDS             26634..27143
                     /locus_tag="PLL88_03250"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOJ00611.1"
                     /translation="MKFGKGDDGFTDLLGGMRVRKDDQYIDLIGEMDELSAWIGVVRS
                     VVHDAATKEMLQNVQEHLSMIMAKISMGHQEKHIDSAAVDSSLQTVEQWIRLLEKETT
                     LPRTFIQPGEKRDGSLMNMLRVVTRRVERRAIATLSSGEQAHSSVTVYLNRLSTLFYL
                     LWLKIEHST"
     gene            complement(27308..28165)
                     /locus_tag="PLL88_03255"
     CDS             complement(27308..28165)
                     /locus_tag="PLL88_03255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOJ00612.1"
                     /translation="MSSAVESVSTFENFLNSQAIVAFLKGQNRTHAHLDWRSPMEWLR
                     RPPSQILHTGAKINGLLALAADPPHMHWVRFFCIRNECDYTYTWRKLFSAITDSYPFE
                     KRDLVAALCYQNWMETLLQTNGWQKAQKVIVLVWQHPPFPKTEKEKDVRIRKMKLDDL
                     PKVAQIDDTSFASFWKFSTTTLTLAHEQSAYSTVAELDEKVVGFQISTADLSRAHLTR
                     LAVSSDARNRGIGAALVRNMLWHFSVPWIRQITVNTQHDNQASIHLYQRMGFAYLGES
                     FPIYQCPIR"
     gene            complement(28162..28869)
                     /locus_tag="PLL88_03260"
     CDS             complement(28162..28869)
                     /locus_tag="PLL88_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ00613.1"
                     /translation="MGSYQANLEMIAQKLTQARFQSGKSMKDCAHAIGSTSAHYKKIE
                     CGEIYPTLPETETLSYFLNVPLFELLSDHKSTDLLVKTGNTAHLIEIRNNVIGTLLQI
                     ERERKNIALVDLAKRCGIQRSRLKRYESGTTRIPLDDLLSLADALSIDLEAFLDQNSA
                     MGIWQKSQQNLRAFSSLPAHIQEFVTDPANPPYLELAQKLKSMQPKDLAAIAEAIQLM
                     VQLLPKDPAQEPDQTQQ"
     gene            complement(29031..30566)
                     /locus_tag="PLL88_03265"
     CDS             complement(29031..30566)
                     /locus_tag="PLL88_03265"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ00614.1"
                     /translation="MHPQRPRGEALAIDNGLVLAVGKNEDVLNLAHGDSEIFDLHGRC
                     MLPGFTDAHIHLLQYGLKLTSIDCETESKQACLRRVSAAIQNAPFGVWIRGQGWNHNV
                     WEEGIGTKEELDSISKHHPIYLAAKSLHASWVNSAALALAGIHKDTPDPEGGKIARDS
                     QGEPTGILLESASRLVEKIIPPPDEGQMRAALIQGQEELIRFGITAVHDMDEWKIYPL
                     LKKMKEEHQFPLRVVKNIPVDHLSDAIQNSLVSGTGDENLRIGWLKLFMDGALGPQTA
                     AMLEPYEASDNTGMLVLSPKELNEIAEQAVGNGICLAIHAIGDKAVRCALDGMARMPT
                     PTAIPHRIEHVQIIHPSDMQRMAALHVIASMQPFHVISDMETADQYWGERCKYAYAWN
                     SIHAAGVSLVFGSDAPVETPNPFLGLHAAVNRQNKRTHRPWHPQQALSLQHALEAYTC
                     TPATLLPGQAVHGKLAVGSRADLTILPVDPFAIPPQSLQTLVPCATMINGAFVWRSSD
                     FMC"
     gene            complement(30603..31472)
                     /locus_tag="PLL88_03270"
     CDS             complement(30603..31472)
                     /locus_tag="PLL88_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOJ00615.1"
                     /translation="MAQRKGLGKGLDALIPSGDFSSAPTAEGVSYIPIERISPNPYQP
                     RAEIHDEELQELCASIKEHGILQPLIVTNDENSTGDFILIAGERRLRAARLAGLDSVP
                     VLVREKMTNQQQLELALIENLQREDLSAIEAAEAYLQLADEFGLSHEEIALRVGKSRA
                     AVTNTVRLLKLPQDVIKAISDGKISEGHGRALLGLENPAAQSAAMKSIVSNGLNVRQT
                     EELVRKLVEHKSSLPPQKKDNPPEIQHIEKQLRSQYGTKVSMNYGKHGGSITFFYYSE
                     EELENLIEQLLHS"
     gene            complement(31491..32300)
                     /locus_tag="PLL88_03275"
     CDS             complement(31491..32300)
                     /locus_tag="PLL88_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ00616.1"
                     /translation="MMAKIYTLVNQKGGVGKTTSAINLGAFLGHYDQRVLIIDIDPQA
                     NATSSLGIDKLTVKKGTYEVMIGATPIGPSILHNPRLKISILPSSPALAGAEVELMDV
                     QKRDFRLREMIDPIVDRYDYILVDCPPSLSILTINGLLAAKDGVLIPVQCEYLALEGL
                     GQLTDTLQRIKRSLYPELNVRGVLLTMYDGRTKLAMDVVQEVHKFFPDRVFQTIIPRS
                     IRLAEAPSYGLPITVYAPTSSAARAYAALAREILQQDGKPVPVVEDSTFDD"
     gene            complement(32310..33152)
                     /locus_tag="PLL88_03280"
     CDS             complement(32310..33152)
                     /locus_tag="PLL88_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ00617.1"
                     /translation="MATNEISYLILGHITQDLTPSGVQLGGTAAYSGLTAAALGHIVR
                     VVTAYPQELHLPTPEKITFQRCASARATQFKNVMQDGKRKQFCYSQADSILPDCIPVQ
                     WRQSDIIHFGPVAQEIPLETVKSFAQHAFLCATPQGWMRSWDDEGLVHPVTWDWAESV
                     LPLMQAVVISLEDVEGDEKVIDEMARLCKILVVTEAEEGARVYWKGDARYFPAPNFPV
                     VDATGAGDIYAAVYFSRLFTTGDAWQSAKLAVQLASLSISRSGLESIPTRQDIRHCMV
                     EVMK"
BASE COUNT         8795 a         7112 c         8440 g         8962 t
ORIGIN      
        1 gaaacgctac atggcaaagg acctgccgat tttcgtgagc attgtttcac tgtttcagct
       61 cacatgctgt tgctgggaga aaaagcccag acacttgatg aggcgtacgc aatggctgaa
      121 aaagccattg tggatggaaa agcatgggac aaatttgtcc aattggtaaa agcgcagaat
      181 ggtgatcttt cattcataga acaccctgaa aaactgcctt gcgggtcaat tcgcgaagag
      241 ataaaagctg aaaatgatgg cttccttgcc atggttaatg cacaatccgt aggagaagcg
      301 gcggtattgt tgggcgccgg gcgtgaaaag aaaggcgatc ccatcgatct gggtgtgggc
      361 attacggtgc attataaagt cgggacactt gtgaaaaaag gtgatgtatt attcacgatt
      421 tacgcgaatg acagagagcg cctgcaggta gcaaaattga aacttaaaga aagtgtcaac
      481 attacggacg aagaatgtgc cgaattgccg ctcttttatg gcgtggtata gcggtactta
      541 attgataaaa gtttctattt atgagataat caaaagcgaa aagctgtgat ggaaacgagt
      601 aaatacgagc gctgtttcca gcgagccgga gaagcgtgaa agaccggcaa acatctcgaa
      661 ttgaaaatcc ttccggagct gcgatctgaa catggcgtgg ttgccatcag taagaaagcc
      721 ggggacaccg atcgttaaaa tggcgcagaa ttctgtgcaa caggcttctg gttgagggct
      781 ctcgcaagag agaatgtggg tggtaccacg ggtaaattcg ctcgtccctg aaacaggacg
      841 agctttttta ttggaggtgt tatgttcaaa ccagtttcat ccaaattaga gattaaagca
      901 caagaagaat ccatcctgga attttggaag caacaagaaa ttttcaaaca gaccttgaaa
      961 aaaacggaag gcagtccgaa atatgtaatt tatgaaggac ctccaactgc caatggtatg
     1021 ccgggtgtgc accatatcct ggcgagggtt tttaaggaca tttttccgcg ttataagact
     1081 atgcaggggt atcatgttga ccgacgaggc ggttgggata cgcatggttt gccggtggaa
     1141 atcgaagtgg agaaaaagct gggcttcacc aacaagcaac agattgaaga gtatggcatt
     1201 gaaaaattca accgattgtg ccgggaatcc gctttttcat acattcaaga gtgggaaagg
     1261 ttaacggatc gcattgctta ttgggttgat cttagtcagg catatgtgac ctataagaat
     1321 gaatatattg agtcagtttg gtggatctta aaaactctgt gggagaaaga tcttctctac
     1381 cagggtttta aagttgtgcc gtattgcacc cgttgtggta ctccactttc agatcatgag
     1441 gtttctcagg aatatcatga cgttaccgaa tctgcgatct ttgttcgcat gccattgttg
     1501 gatgaggaag atacctcgtt gctggtgtgg actaccactc cctggacctt gcctggaaat
     1561 gtggctgtgg ctgccggaag agatatcacg tacgtcaaag ttgaaaggga cgttcctaat
     1621 ggggaagaac acgcaaaaga aaaattgatc ctggcggaag ctcttgtgga aaaggttttt
     1681 ggcgaggaaa aagttgctat tctcgaccgt ttcaagggga aacagttgaa ggggaaacgc
     1741 tatcagccat tatttacatt cttgccagtg gaaaagccag cccattacgt tgtgctgggt
     1801 gattttgtca ccaccgatga tggttccgga ttggttcaca ttgctcctgc gttcggcgcg
     1861 gacgatatga acgttgccaa ccaggaagat ctgccagttc tgatgactgt aaaaccggat
     1921 ggtactttta tccgtgaagt ggatcaatgg gcgggtaaat acgtaaagga tgcggatcct
     1981 ttgatcattg atcatctcac agcgcgcggc ttgcttttta aggtggaaaa acacactcac
     2041 tcgtaccctt tctgctggcg ctgcggcact ccacttttgt attatgcacg ttctacctgg
     2101 tatgtccgta ccagtcaggt aaaagaccgc ttggtggcgc tgaataacga gatcaattgg
     2161 tatccggagc atattaagaa tggacgtttc ggaaactggc tagaaaacaa catcgattgg
     2221 gcgttgggtc gcgaacgctt ctggggtacc ccattgccgg tttggcaatg tgaagattgt
     2281 ggaatgcaaa cctgtgtagg ttccgtggag gagttatcca aactggtagg tcatgacctg
     2341 accggattgg aactgcatcg cccatatgtg gatgaagtgg tctttgattg cccggagtgc
     2401 catggaaaga tgcaacgcgt accggaactg attgacgtgt ggttcgattc tggctccatg
     2461 ccgatcgcac agtggcatta tcctttcgaa aatgttgagg aattcaagca gcaattcccg
     2521 gctgactata tctgcgaagc tgttgaccaa acacgcggtt ggttctattc gctgcatgct
     2581 atctcaaccc tggtgttcga tcagaaatgc ttcaaaaatg tggtctgcct gggcttgatc
     2641 ttggatgaga acggtcagaa aatgtccaaa tcgcgcaaga acgtggtgga cccgtgggaa
     2701 gttattgaac agaacggtgc agatgccact cgctggtatc tgtataccgc cagccctccc
     2761 gggcaggaaa ggcgtttttc aaccagtttg gtatctgagg tgatccgcac attcatgctg
     2821 acgttgtgga atacctattc cttctttgtg acctatgcta atcttgatca atggcagccg
     2881 gatacccaaa agaaagatat tgaatattcc cccttagatc actggatccg ctctgaactg
     2941 catgcgctcg tacgggatgt gaccacggcg tttgagaact atgatgtgat caacgccacc
     3001 cgcccaatcg aagcttttgt agaccacctt tccaactggt atttacggcg ctcacgacgc
     3061 agattctgga aaagtgaatc ggatggcgac aaatatgccg cctatgccac attatatgaa
     3121 gccctggtca ctgtgagtaa actgatcgct ccctccatgc cctatatggc ggagaacctt
     3181 tatcaaaatc tggtgcgctc ggtggattcc aacgcggcgg tttccgttca cctgacggca
     3241 tggcctcgat gtgatgaaaa agtcgtcgat gctgctttga accatgagat ggaagtggtc
     3301 atgaagctcg tgtcgctcgg gcacgcagcc agaaataaat cgggcattaa agttcgccag
     3361 ccgttggctg aagctgcttt tgcacaaagc gctgcggatg atgggggtat cgtcgaaaga
     3421 tatgccgacc ttctggaaga tgagttaaac gtcaagaagg tccgtttact cagtgctgcc
     3481 aatgaagcgg tggcgtatga gcttctgccg ctgccaaaac agttagggca gcggtttaaa
     3541 ggggtttttc cgaaggtcaa ggaagccatt ttaaaattgg atgctgaaaa gattgcagaa
     3601 gctgtatcga aaggtgaatc cttcccggta gttgtggatg gaaaagaaat ctccatcaat
     3661 ccggatgagg ttgaaattca cattcaagct aaagaaggtt ttgaagtggc gtcggaaggt
     3721 gcatacttgg cggctctggt gacaaccatg aatgacgatc tgatttacga gggcttggtg
     3781 cgggaattca tccgccgcat ccaggaatcg cgcaagcagg ctagctttga aatcgctgat
     3841 cgcatcaagt tatcattctc cgcatcggag aaactggcga aagccatcga agagtatcgc
     3901 gactatgtga tgaatgaaac actggcaatg caaatggatg cgaagaacca tccggctaca
     3961 ctgccgaatg caagtgacga atttgacgga gaacaggtca ctatctggtt ggagaaaatg
     4021 aggtagcatt gttcgggaat gccgagggct gatcggcaag atcagccctt ggtgtgttaa
     4081 tcgcggatga aatgcgggat aacatccggg gtgatgaaac tgaattccac caacccggca
     4141 aatttcccat tttgaaaata tgggatttgg tagatcaact ttttgattcc attcttctcg
     4201 atggtgtaga catttgctgt agtaccctgt gaaagctgtc ttattttttc ctgcgctttg
     4261 gatgggtgac agtcgaagag gttcttccca ataagatctt tgccgccgct gcttgcaaag
     4321 gtatctgccg ctttttgatt catttcaatg atctttcctt cggcatcgga tactgtgatt
     4381 gcaatcggaa tgttttctag ccaggatgga tgagacatga ttgctcctaa gatatttcct
     4441 ttgttttgtt ttaatgatgg ttcttttgga accatattat atgcagaaaa caagaatggt
     4501 ctatgcaggt tagactgaat gaaaataatc aattacaatc atttgaaatt cattgtggag
     4561 aagtcatttg aagaagaaca tcaaagcata cgcttttctg tttgtcctgg ctggcctgat
     4621 cattgcgttg gatcagttta caaaaaattt ggtacgaacg aaccttgctt atggtgaagt
     4681 ctggtcaccc tgggaatggt tgatgcctta tgcgcgtgtg gtccattggt ataacaccgg
     4741 tgtggcgttc gggctgttcc agggtatggg attggttttc acttttctcc ccttgatcgt
     4801 ggttgtcctt attttcatct tctatagccg cctggctggt gataattggt ggatgcgcac
     4861 tgcaattggg ttggagtttg gcggagcgat tggcaacctg attgaccgta tcacaatcgg
     4921 gcatgttacc gattttatct ctgtcgggaa ttttcctgtt ttcaatgtgg cagattcggc
     4981 aattacggtt ggtgtgtgtg tattgttgat tgtgctctgg aaagaagaga agaagaattc
     5041 tataaaacct gctcaataat atgtctgtaa atggtgatat cagagagttc gttttttcag
     5101 gtgatactcc tgttcgtctt gataaatatc tggcagaggt tcttccttcg caatcccggt
     5161 cgcggctaca ggcattgatc aaaagtggat tcgtcaggat caatggcagc attgtcaaca
     5221 aaccaagcac gctgataact gcaccgtgca agatcgaaat tgagatccct cccgcgcaac
     5281 caggtgaatt ggtccctgaa gatattcctt tagacattct ctatgaggat aagaatgtga
     5341 tcattgtgaa taagccggca ggcatggttg ttcatccggc gcacggacac cccacaggca
     5401 cactggtcca agccttgctg gctcataccc ctttcctgca ggggattggc ggagttcaac
     5461 gcccgggaat tgtgcatcgt ctggaccgcg atacctccgg tgtgatcctg gtcgcgaaaa
     5521 acgaaaaaac ccaccgctgg ctgcaagacc aattcaaatc gcggaaggtt gtaaaaacct
     5581 actatgcgtt ggtagatgga cacccaccca cacccaccgg tcggatcgag gttgctattc
     5641 aacgggatcc acgcaataga aagaagatgg cagtggcgta tggtgaaacg ggaagggttg
     5701 cggtttccga ataccgcatg cttcattcct acaaaaatca cgcattgttg gaagtgagaa
     5761 tatttaccgg cagaacgcat cagattaggg ttcacatggc atatcttgga tgtccggtgg
     5821 tggctgatac cgtgtatggg cataaacacc cctcattgcc attggctcgc cagttcctgc
     5881 atgcccgatc catttctatc atattgccag atcagcagga acaaagagaa tttgttgctg
     5941 aaatgccggc agatttacag cagattctta acaatttgat ctaatggaga atccatggaa
     6001 cagaaaatga cctatatcga tttgcgttca gatactgtta caatcccaac ccctgcgatg
     6061 cgtatcgcaa tggaaaatgc tgtggttgga gatgatgttt atggtgaaga tccaaccgtc
     6121 aatgaattag aaaaaaaggc ggctgcccta ttggggaagg aatctgccct gtttctgtca
     6181 tccggaacca tgggaaattt attagcgatt ctggtggcat gtaaacgggg agaagaagtg
     6241 atcatggggc aagcggggca tacctttctg cacgaggtgg gtgggatatc tgccttgggt
     6301 ggggtctttt caaacacact gcccaatcag ccggatggca ctctgcatct cgaaatggtc
     6361 agagaggcga ttcgcgggga tgatatccat gaaccgcgca cgaagatgat cgtgatcgaa
     6421 aatacccaaa atcagtgtgg tggggttccg atcacgttcg attatatgaa acgtctggga
     6481 aatctggcga aggaacacaa tctgtttctt catgttgatg gggcgcgcat tttcaatgcg
     6541 gcggttgcac tgcacagttc agccagagaa ttgaccgcca tggctgattc gataacgttc
     6601 tgtttgagca aaggactgtg tgcgccggtt ggctctgtgt tgtgtggatc tgggggattt
     6661 atccaggaag cacggcgtta tcgcaagatg ctgggcggag ggatgcgcca ggtgggaata
     6721 ttagcagcgg cgggcattgt agcactggat accatgatcg atcgtttggc tgaagaccat
     6781 gcgcgcgccc tccaactggc tgaaaatctt gcggagattc ccggtttttc gttgcccaaa
     6841 gggttacctc gttcgaacat ggtttttctt ggggttgatc gaacggtttt tccagatttg
     6901 cagatgctgc aggaaaaaat taagaaaaaa gggatcttat ttggattctc aggttctgat
     6961 gaaattcgcc tggttaccca ttactggatc accgatgaaa gtattcgctc tgtgattgaa
     7021 tgctggcaaa acctgcaggc gtaaatcagc cataggcgga aatgcttgtt ataattgaaa
     7081 tcttatgagg tgccattatg actgaattta ttactttgaa tcagatcaat tcagccgtta
     7141 cggtaattag aaaaagagcc aaacaacaac cgaaagttgg attgatcctg gggtcaggtt
     7201 tgggtgatct ggcagattca gtgattgccc cagactgtgt tgactacaaa gacatccctg
     7261 gatggccagt ttccaccatt caaggtcaca aggggcgact ggtgatcggc gagctggcag
     7321 agattccggt gttgatcatg cagggcaggg cacattatta tgaaggatat tccatcagcc
     7381 agatcggatt tccaattcgt gtgatgcgaa aacttgggat cgaaaccctc atcgtgacca
     7441 atgcggctgg tgccataaat ccagctttca agcccggtga tgtgatggtg atcacggatc
     7501 atatctcatt gatggctatg ggtggggaaa atcctctgcg agggcccaac ctggatgaat
     7561 ttggtccgcg ttttcctgat atgagtcgct gttattcaac agacctcatc aacaaagcga
     7621 aatccgtata tgcaacaaac aatgtcgatt ttcattccgg gatttatgta tgccttgctg
     7681 gtccttcctt tgaaacccct gctgatctgc gttttctcaa actgatcggc gctgatgcgg
     7741 tgggcatgtc aaccgtgccc gaagtgatcg tggctgcaca ctctggaatg caggtactgg
     7801 gtttttcggg gatcagcaat aaagccaatc tggatgggag tacgatcact acccatgaag
     7861 aagtgctgca ggctggcaaa gagattgcac ccaagttgga gaaaataatc cgaggtgttc
     7921 ttgcaaccct ttgatggatt ttcatattat tgtgggtgaa ttagatgact ggattgctcc
     7981 gcttcttgaa agagtttgaa ggtggaatct accttttgtt attggtcctg gcgctggtta
     8041 tgggcagtcg gctggtatcg gcaaaccaat tgaagaagaa gacccccttc ggcttggaaa
     8101 aagagcttgt ccagaagcgt attaatgcaa ccgttgccta tttggcgatc attgggatac
     8161 tctttatagg acagatattg ttggtgtctg tggcatctgt gcgctatccc ggtttgtaca
     8221 ctattacgac tccaacaagt ggaatcattc ctacccccac cgattttttc caggcgatgc
     8281 tgcgcatgga ggaaactcct gaggggttgg aacaaacgca aaccgcctca gccttgacgg
     8341 gttgtattcc acaacagatc gaatggatct ctccgaaatc tggagacgag atatcgggtt
     8401 ctgtttcatt atcaggcacg attaatgtgc ccaatatggg tttctataaa tacgaatacc
     8461 gtcagaaggg tcgggaagat tggattccca tcgctgctgg caataaagtg atcgtagcga
     8521 gtgaattggg tggtagttgg aataccgaac aactggaacc aggattgtat gaattgcgca
     8581 ttgttgtctc ggataatcaa aataacctct tcgaaccatg tgtgattgag gtaaaggttg
     8641 tattataaaa gggaggagac agaatggctg aagtgataat ccgaaccgtg gtttcgccag
     8701 atttctccat tttatcgagt tttaaacact ccctcgaaac ccaaactgtc tggcagatgg
     8761 accaagaagc agtggatgat tctattgcag tcagttttcg agagttgaaa ttgccgagac
     8821 tgatgaaatt atcttatcca cgttctgctc agagtttgtt cgaccgctgg aaagatttat
     8881 cgatcattct ggtggggtgt attgataatg tgccaatcgg atacatttca tgttcttcca
     8941 ttcaagctac ctcgaatgtg tggatcaaag acctggtcgt tcatgagcgt tggcggcgcc
     9001 agggggtggc gacatccttg gtaaaagcca ttggagtctg gggtgaagag cgcgggttga
     9061 aaagaataac ccttgaaatg agctcgaaaa attacccggc aatttgcctc gcaaagaagc
     9121 ttggttttga attctgcggc tacaatgatt attattatga aaacaacgat attgctgttt
     9181 tcttcgctcg tttgatcaga tagggggaac aatggcgaac agcgtggaaa atgtaaataa
     9241 ttgggttctt atttttaaaa cagtcagtga tgtccttact gctggtttgg cgattaccac
     9301 tttctctcta tttctctatt ccctgacttt caagattcgc gaccagttga ttaatattta
     9361 ttctggtatc ttgtttaccc tgatcctgat cttcggcgca gatgccttcg gaacgatcac
     9421 aaacgagaaa attgcattat tgaatatttt gaagatccat tggctcgggc tgatcgctat
     9481 tcctacactg tatttcctgt tttctgatgc attacttgct ttaacgggta agcctagcca
     9541 tggaaagcga cggattttcg gcttcatgat gattgcaacc agttgtttct tttgtgtact
     9601 tttacttgct gacggattgt tgggagaatt gattgttgat caaaaacctg cgccttactt
     9661 tttgcgaacg aaagccatgg atttatattt ggctttcttc ttctcctcaa tcctcctgtc
     9721 tctgtataat tttttccggg ctattcgccg taccaactct cctaccagca agcgtcgaat
     9781 gatctatctg gttgtaggag caatcggtct gtttttaggc cttttcccat atttaatttt
     9841 tgaatcatcg atgggtgtgc tgaactcgtt aaccttctgg attctgtcat gcattctcta
     9901 tctgggaagt tggatcttat tgagcacaat gacatattcg atttctttct tcggtttgtc
     9961 tgttccggat cgtgaaatta aaagtcgatt gtttcactgg atctttcgtg gaccgctaac
    10021 cgccagtgta accttgggat tgacaaccct catccggcgc attggttatc tacttgataa
    10081 agatttgctc atgttcgaga ttctcgccat ggtggcatgc attatgttat tggaacatgc
    10141 gtacacaatt ctgttcccta agatggagaa aatactgttc cgtggaaaag atttgcagga
    10201 aatggaaagg atccgctctc tggaagcgcg tttattcaca agaaatgatt tgaatcaatt
    10261 cgcggaaatc atcctggctg cgttgtgcga tcgtatgcgg gcaagcaacg cagtactctt
    10321 tttgaagccg aacggtggct atgaaaactc cgttgaagtt gggaatcctg cgcgcattat
    10381 agaaaaagtt aagaaggcga attatcagtt taatgaggat tgggaaaaga agacgatctt
    10441 ctattggaat ggaattgtca tcatacccat caaagatggc gatgaacgtt tgaataattc
    10501 attgatatcg gggttcatcg ggttggataa aataaaacga acggatctcg atcctgacca
    10561 ggtgagagcg gtgaatcttt tattaagcag gttgcacact gctattgtcg attaccagca
    10621 acaacaaaaa gtcctttact ctttggagtt gcttgagccg caaatggttg agatccaaaa
    10681 tctgttagct gccgggcggt ttacgaccat cgctatgccg atcaacctgg aagacactga
    10741 agttttaaac agatgggcca aagatgccct ctcgcatcta tggggtggtc cgaagctttc
    10801 agggaaccca ttaatgcaac tagcaatcgt tcaacagaag gcacatgaga gcggtgaaac
    10861 gttgaccaaa gccctgcgcg agctgctcca gggagcaatt gcctctttaa aacctgaagg
    10921 agagcgccaa tataccaatg attggttact atacaatatt ctcgatttta agtttaatgg
    10981 gaatatgaaa atgagagata ttgccaagcg gctggcactt tcagaagctg acctttaccg
    11041 aaagcaacga attgcaattg atggggttgt aaaaaagatt attgatatgg aaactacttt
    11101 attaaaaaac aatcaaaaaa taggagggaa aaagaatgac gatgaaaaaa agagaagaac
    11161 ctgaagatac tgcacccgta cttgatgaat cctggtggaa ctcattgctg gatgctgcaa
    11221 atcaatatca ggaccatgaa aagacaggtg gcgaccatca tctggaaaat gaaaacatcg
    11281 aaatcgattg gaaaacaatg gaggagtatt tccggaatga tgaggtattt tccgttcagg
    11341 tgattggtta taacaaaggt ggtctgctgg tacgcggtga gaattttaag ggttttattc
    11401 ccaattctca catcaaagaa gtttccaaga tccaatcagc atcggcaaaa ttccatgcat
    11461 taaagcaatg tgtcggcaag gatgtgcagg tgaaggtgat tgaatgcgac cagaagatgg
    11521 ggaaactgat cctttccgaa cgtgctgctg aaaacgcccc gggtaaacga ttggatgttt
    11581 ttgaagacct ttccatggga agtgtggtgg agggggaagt aacgaacata acgaagttcg
    11641 ggatattcct ggatttggga gggattgagg gtctggtgca cgtttctgaa atatcctggg
    11701 gaaaagtgaa ccatcctgct gatatcgtga agatcggtga taggatcaaa gcgagtgtga
    11761 tctctgtgga tcgagaaaag atgcgcatcg ctttgagcat gaaacggcta ttaccgaacc
    11821 cgtgggtaga attcaacgaa cagtacatgg ttggggatgt tattaccgta acgatcaaca
    11881 aggttgttgg gtacggcgtg tttgcgggca ttgagaaagg gatagaggga ttggtgcata
    11941 tcacagatat ccaatcgaaa gatgccacct taaccattaa tgatctcaaa gtaggtgaga
    12001 agatcatcgt aaccatcaaa gcaatcgatg tggaaagacg ccgattgagc atgggattca
    12061 taagcaaatt tgaataaatg cattatgatt aattcatgaa aagcaaatcc aataatgtcg
    12121 tgaaagtgct ggaatatttt catagattct tgaaagacat tattggaatt cttttaattt
    12181 ccatcgcact tatttccatg ttgggctttt ttcatttatc tcagggaaag gtgttaaatc
    12241 gccttgttga gatcattgaa cagggatttg gttggggatc tatatttttc ttattttctc
    12301 tgctgtatat cggagtgatg gtcctgttcc gacgggttga gagttttcca gatctgaacc
    12361 tcaagcggtt gctgtacata gaagtattct tcttttccac tcttggtttc atggcaatct
    12421 tcggcgggaa ttcgctcgtt cgcgcaactg atggaaaaga cggcggggtg atcggatggg
    12481 gcatcgcaaa gatcgtaagc tctgttttgc cgctgccggt ttgctcgatt ttattgtccc
    12541 ttgttgccct cctatctttc ttggtagcca caggattact gcgcaagatt gtgagcaaaa
    12601 tacaaacaag agctgttcca gagcataaaa tagaaaaacc ggaaggttca ttgccacctg
    12661 atcttcaact taatcatcaa ccctctacag gaactcgaac cagttcgtct attccaaaca
    12721 tggaaaatgc gcagaagaat ttaccgccgt tgtcgcttct ggtggagcaa aaaagccatg
    12781 ccactgacga aggctatatt aagcaaaatg ctcaagttat tgaacagacg tttgaagaat
    12841 tcggaattcc ggcaaacgtg gttgggtatc gcattggacc gacagtcatt caatatgctt
    12901 tagaaccagg gtacattgat aagaccagtg aagacggaga agtgagtaaa cggaagatca
    12961 gggtgtcgca gattgcctct ttacaacgtg acctggcgtt ggctctggga gctgaacgtt
    13021 tgaggatcga agccccgatc cctggttatt cttttgtagg cattgaagtg cctaacaagt
    13081 acagcaatat ggtgcgtctg cgaagtgtgc tgaacagtga tgtgttccag aaattgaatt
    13141 ctcccttgcg agttgcttta ggattggatg tatcgggaaa cccgattgtg gctgatcttt
    13201 ccaaaatgcc gcacctcctg atagctggca ccaccggatc cggaaaatcc gtttgtatca
    13261 gttctctgat ctcaagtttg gtgatgtgca acaagcctga agaccttcga ttggcaatcc
    13321 tggatcccaa atgggtggaa ttatccattt tcaacggact gccgcacctg ttgggtgaga
    13381 ttgaaacgaa gacgaatcgt atgctggctg ttttggagtg ggcttcttct gaaatggaac
    13441 gtcgctataa gatatttgaa gaggtgaatg cacgcgatat caccgtctat aatagcaaag
    13501 ctggcaggcg cggattcgca ccacttccgc gaatagtgat tttgatcgat gagttagcca
    13561 gtcttatgat gtcatctccg gaacaaactg aagcgcatct ggttcacctg gcgcaaatgg
    13621 ctcgggctac cggtatccat cttgtcattg ccatgcagag acccagcact gatattctga
    13681 caggcattat caaggcaaat ttcccggcga gaatcgcttt tatggtcgca tcctcggttg
    13741 attcgcgcgt cattcttgat atgaatggcg cagaaacgtt attgggaaag ggagatatgc
    13801 tgttcctgga tcctgacaat ggagcgccaa aacgtgtaca gggtgtgatg gtagatgata
    13861 tggaggtcaa gaacattgtg gaatattgga attccatcta cccaccgggg caaaatgggg
    13921 aagcggaagc accctgggaa aaatttatcg tcaatcaagg cgaggatggc gatgaattaa
    13981 tcggaagagc gattgaacta ttgaaaggcg aggggcgagc cagcgcttcg ctattgcaaa
    14041 gaaaattgag aattggatat ccgcgtgctg ctcgattaat ggacgagttg gaagaaagag
    14101 gaattgttgg tccgccagaa gcggggggga gagaccgtga ggtgattctg gatgatgatc
    14161 gtccttatgg tgacgaggaa gaaagttatg actgattgaa cttgacagga ttcctcggtg
    14221 aagataggat tgagcgttat gtcaaatggg tgtgatcatg accttcactg atcgactgcg
    14281 tatcattttc caacgttttt tggatagcgt ggggcatact cttgtccatt ttgggatttc
    14341 agccaatgcg atcaccctgc tgagtttagc aggtagcgtg atcgcttctg tttttatcgc
    14401 caggggaaat ttattggctg gtgggatcat tttagcatgc accggaccac tggatgcgat
    14461 tgatggcgcg gtggcgcgtg ctgggggcaa gatcacgagt tttggcgctt tttttgattc
    14521 tgtttccgat cggtattcag aactcatgat ttttggggga ttggcttggt tcttcctcaa
    14581 ccggctggat aaaatgggaa tgatcctcac ctttcttgct gccgcgggtt ctgtattggt
    14641 ttcgtacacc cgcgctcgcg cccagggtgt agggatagat gttaaagccg ggtttatgac
    14701 cagagtggaa agatacgtag tgcttgtccc tggaatcatc tttcgaatcc ccaaagtatc
    14761 attatggatt atagcaattc ttgcgaacct gaccgcaatt caacgtatct ggatcgtttg
    14821 gaaccagacg aaaaatacag aaaaggagaa ataaaaaatg tcagatggtt tttatattgg
    14881 accgatatat atctattatt atggcgtaat tatcatggtt ggggctttgg ctgcgttgtg
    14941 gctggctatt aaagaagcaa aatatcgcaa gcttgaccct gagatcgttt gggatgtggt
    15001 gccctggttg ttgattgtgg gcatcgtggg ggcgaggctt tggcatgttc tgacaccttc
    15061 gaaatcaatg ggtgcaggaa tagaatatta cttttccaac ccaatcgaga tcttgaaaat
    15121 ccgcaagggc ggcttgggta ttccgggtgc catcctggga ggcatccttg ctttagccat
    15181 ttactgccgc aagaaaaaac tcaatttttt cacctgggca gacgtgatcg tacccggact
    15241 ggcgctggca caggctattg ggcgttgggg taactttatt aaccaagaac tatatggagc
    15301 ccccacggat ctaccatggg caattcacat cgatgaagcc aaccgttttc ccgaatatgc
    15361 ccaatattcc acgtatcatc cactattttt ctatgaatca ttgtggaatc ttttaaatat
    15421 gtacttgttg attgtcctcg cgcggcagta caaagagaaa ttgatcccgg gtgacctgct
    15481 gtttacctat ttgatcgttt atccctttgg aagattcatg ctggaattct tacgattaga
    15541 tacttccacg ataggaggga tcaacattaa tcaaattacc atgctggtgg tgatgatatt
    15601 tgcaattgtc gtgttgatca tacggcatcg aaaacctcag gaagtgaccc aagaacaacc
    15661 ggtgaatgaa ggacaggtcg aaagcgaaga ggaaaaagac tagaaaaaga gctgcaatga
    15721 aaacagcgtg tggtaaagaa ggaatctcaa gcaaacattg gatggttggc gaacgatatt
    15781 gtcgataagc tgttagccat gggaagctgg aaacaagtat gcagagattg cctgcggtca
    15841 aagcgaccca acccatggag tgggcagatg cagcacatgg gctgaagcgt gaaacggacg
    15901 aatttgtgtt ccgttgcatc gcatgccgat caagcattat gagaacacct gtggaatcat
    15961 cttccacagg tgttctctga tatatccatt atttttgtcg gcaatggtgc cgcttaattt
    16021 aatcatcact tttggcgtca tgctgatctg ttgctttggc tgacaatatg ccttgcaaaa
    16081 caaaggaagc caacaatgca aatgtgatca accaccaccc aaccgcttca aaaacatctt
    16141 ccggaaccat aaacgtatat cccaggtagg agaaaaatag tattccctgc agaattttga
    16201 aagcattggc atcggcttga ttttttaatg ctaccagtcg ctcatcattt tcactgatga
    16261 tgatattttt catcttttgg ggattttttt taatggttcg cagtttcacg taagaaatga
    16321 ctgcaataac caatggagta aaggagagag ccacaatagc cttattgttt ggcagaagct
    16381 tgatatccat taactcaagc agaatgccgg ttgccaacag cacgatgcca atgatcaacg
    16441 agacaagcat aatcattgtg ctttttttaa ctgtcttttc aatattcata gtttattcct
    16501 cccaaaataa atcatttaag gttttgttga gggctttgca tatttctatg cacaacttca
    16561 ggctgggatt gtacttgcca gattcgatga gattgatggt ttgcctggtt acacctacca
    16621 tctcagccag atgttcctgc gatatgtctg attccactct cgcgatcttc attcttttat
    16681 tcttcaaaaa atcccttcct ttctctagtt ctatgaacag catactattt atattgcata
    16741 atgtcaagta tatattgctt tttgtaacat aaaaataacc aaccaatatc ggcatcagtc
    16801 aagacctgta tctctttaaa gcgttttata ccccatgcct ggatttccat accatgttta
    16861 taccgatcga gactttacgc cttttgaagg tgaggaaatt gtagataaca actgtgggcg
    16921 gtctggtttg ggatacaagg gaagtggctt cgaagcgcta taccgtctga caagtgcggc
    16981 aggaagcagg aaatggaaat cgtctgcaac ggtgcgggta tcctgcgcat cctcgaacta
    17041 ccccgtgaag tgttgtgcaa aaacagagaa acagcgtgcc cttccggctg cgctgtttcg
    17101 tgtttacgct acgttcctgc tgatgcccgc tcttgggcgg tttcttttta ttaccagcct
    17161 ctcaagaatg gctcccaatc gatgttttgt aacacgtgtt ttccaaagat gtattcagcc
    17221 gatgagggag gtgcttttgg ttcgcgcagc agacgcatat tggtcatttc aagtgttcgc
    17281 ccacctttca gatgattgca tcgcgggcat gcagtaacga tgttttccca cacctggctg
    17341 ccgcccaaat gctttgggat gacgtgatcg atggttaatt catcgcttgt tttaccgcaa
    17401 tactggcagg tgtaattatc tcgacggaaa acttcttttc gtgtgagatg cacctgcgga
    17461 cgggggcaat gaaccatgta ctgaagccgt ataatggagg gtttgggaaa gtcacggctc
    17521 acagtgcgta taatcccgcg accatttaaa accaaaatag ccctatcgag aacaatcagt
    17581 ccaactgcgc gacgagtgtt gcatacattg ataggttcaa aatttgcgtt gagcactaga
    17641 accgtttcca taaccgttgg acaggattat agcatgagta atgctaatgg gaataaaatt
    17701 acttggaatc gactaaaatc aaaggactgg tataatgctg gaaatttcat atccgcatgt
    17761 tggaggtcaa tgtgaacatc gatgaacagg ttgcgcttct aatgcaaggg accgaatatg
    17821 gagacgctga tattcaaaag aatatggcaa gcgaactgaa agagcgcctg atccaggctg
    17881 agaaggacaa acgtcctctg cgcatctatt gcggatatga ccccaccaaa gctgatctgc
    17941 atcttgggca cacggttact atgcgtaaac tacgccagtt ccaagagctg gggcatcaca
    18001 tcattttctt gatcggggat ttcacagccc tgatcggcga tccatctgac aaggatgtca
    18061 cccgtccgat tctcacccgg caacaggtgg aagaaaatgc gcgcacttat gcagaacaag
    18121 cttttcgaat tctggatagg gacaaaactg agattcgcta caattctgaa tggctttcaa
    18181 agctcagttt tgctgatctg atcaagatcg cctccaactt cactgtgcaa cagttcctgt
    18241 cgcgggaaaa atttaagttg cgctgggaaa agggggatcc gatctatgtg cacgaaacgt
    18301 tctatgcgtt tatgcagggt tatgatgcgt acatgctgga tactgatgtg caggttgggg
    18361 gaacggatca gttctttaat attatgatgg cggggcgtaa agttatgagc gctatgggaa
    18421 aacagccgaa catcgccatt actgttgaga tattgcccgg cactgacggg gaagtgaaga
    18481 tgagcaaatc gctcgggaac cacatcccca tttctaccac cgccgatgat atgtatggca
    18541 aaatcatgag catccctgac aaagccatgc cacgttactt ccgcctggta acgcgttgga
    18601 ctcctttaga ggtggaaacc cttgaaaagc agattaaaga tgggaaagtg cacccgcgcg
    18661 atgcgaaaat gaaacttgcg tttgaggtga cagatgcttt ttatggaaca gaacaagcca
    18721 cacaagcgca aaacgatttt gttcaaaagt tccagaagaa agaaatgccc gtcgatatgc
    18781 ctgatttccc tatcacaaac ggacaatctt tattggaggt aatcgttgcg gcacagatcg
    18841 tgacttccaa aagccaggca aaacggctct ttgagcaggg cggtgtatca ctggatggtg
    18901 aaacggtaaa agattgggca atgcttgccc aaccaggggt tttacaggtt gggaaacgta
    18961 attttctaaa actggtcggt tagttgtcca tttgctgcat gaaatcgtag atagcgttgc
    19021 tgatggagag cgcttgtgca gcgctggcat tttccagaac gatcaccaca accgttcgct
    19081 tgttttgcgt cccgtttacc gtgccttgca tcacccattg ataaattcca ttcgcatctt
    19141 ctgaaaaacc gctgaattcc catgaatttc cacacttttt aaaaaatgaa tccatcaaaa
    19201 aatctatttg tgtacgggaa ggtgatgcag cgtcaatttc ttgcaggttc tcgcctgtct
    19261 gcaagctttg attattaaga tcgagaataa gggaaaggtc tactgattcc cctgcattgc
    19321 taaccagcgc tgctgctctg gcgaccatta atggagaaaa gcgaatctgg tcgcttccgg
    19381 caacataggc aacaggcacc gcaagatttt ctgtaaaata tgttgctgaa agaggcaagc
    19441 cgatcgcagg gggagtaaaa aatccaaacc cagccagcct ctgccgtagt tgttctccag
    19501 ataaattccc caccaggctg aacagtgcag cctgacaacc attttgcatt gcatcgttta
    19561 tggctgcttc tccttgtaat gcaagcaaac aggggctttg cgaattctca gattgcaggg
    19621 agaggaggga ggaagaaatg agaccctgca attcttcttc gttgaggatg aacgggatca
    19681 agatgcttcc cgctggataa gatccctgca tcaccctgtt taaaaatggg gctccgccat
    19741 cctggttcca ggcataccag ttttcatcca actcattggc gtcaaatcca ggctgagacg
    19801 aacttgctaa aatttctccc gatgcgatat ctaaaacgat ggctgctccc tgatatcctg
    19861 tcattaattc atccagctta cgctggatat ccaaatcgag agtcagccgt acatctatcc
    19921 cttccggagg ctgatcgtac accagatgtg tgaaccagat ggtggattga gggtatccct
    19981 gataaccacg caaataggcg ttcaggtatt cctcgatgcc agcgtgccca tagcggatat
    20041 gacggaatcc gacggtggta gaaagctggg ggtagggata gtaacggtaa aatgacccta
    20101 cttttccttt ggtcaccgcc aaaggaaaat aatctctatc gtagatggtc cctctttcaa
    20161 cgaactggtc ggcgtaaatc aacctcgggt tatctgagcg atactgcaag ttatctgctt
    20221 tgacataact ccaccagaac aggacacctt cgaccaatgc gattgcgatc atgaaaagtg
    20281 ttcccaatac gatcagaggc tttttgatca attctctctg aactgaccgc ccagcgtttt
    20341 cttcctgcga agtgtccatg cagaaaagca ttgagatcgc cagaaaagcg gaaatgtaag
    20401 aggaaccgcc ataagaaacg aatggaagcg ttacgccggt gattggcaat aatcgaatgt
    20461 tccctccgat gatcaaaata gattgtgtga tcaagtaggt ggtgacgccc gcggcaatat
    20521 agctttggaa aggattgatc gcgttgcgcg ccatcttgta accgcgttcg agcatcaaag
    20581 ccaaacaaat catgaagcaa gaggtgccga tcaggcctgt ctcttcggcg attgctgcga
    20641 aaatgaaatc agaatgtgcg agtggcacaa cagtaggaaa acccagccca ggtccgctgc
    20701 ccaatactcc gcctgcggcg atggaaagga tggattgtat tacctgatag gaaccgccac
    20761 tggggtcttg ccaggcggta agccaggcag atatgcgggt ggaaaaaata tcgaagaatt
    20821 gataaccaag aacaccaaac agtaaaaatg caaggacgat ggaaccgaaa acacgctttt
    20881 gaccggtata gaagaacagg tacgtggcat agatgatgac gaagatcaac gcggttccaa
    20941 aatcattttg gtaaaccagt gtcccgcaga ttacaagaaa aacgaacagg gtgggcaata
    21001 tgctcttcag agtacgttgc ttatctttct cttttgcgaa atacgcggat aaaaagataa
    21061 taaagaacag tttaaggaat tccgacggtt ggatgttgaa tcccaaaata cgtaaccaga
    21121 gggtaggtcc atcaccacct gggaagatcc cccaaaaaat cgtagccgtt agtaatccca
    21181 acccgaggag cagtccgacc aggttatatt tttgcagcaa ttgcatcacg gattgttttc
    21241 gggtcaccag gaaccaggtt aagagtaacc ctgccagtat ccacaaagtc tgacgcaaac
    21301 cgtaaaaagt gcccaagcga aagatggtca gcaaccccca accactaaac aaataaatga
    21361 tgggcaagag aaatggatct acattccgcc cgcatttttc aagagtgtgc tgtaaaaaga
    21421 agaaaccaag cgagaccagg gttaacccga tccagtggaa ccaccgaagg tctacctgcc
    21481 aggatcgata tttcgccacg ggcgaaagtg taagaacagc ggcaaaaact aataaaaaga
    21541 gagccgccaa tctcatcagt tgcttttgat tttgcatagg gttattctcc ttcataagaa
    21601 ggggaggtag ggcaatacct tttattcaaa aaggatctta aggttttcct gcgtactctc
    21661 gggaatgcgg ttttgttcgg tgagcagagc attctttaac gcatcccaac agtcgcagtg
    21721 agatactggc ggaaggatat ccataagatt caagattgcc ttttctgcgt tggatgtgtt
    21781 gttttttgat acctgaagta ccatttccac agtaaccggt ttctcactga catgccagac
    21841 atcataatcg gtaacatgtg ccatggtgac gtaacacatc ccagcttctc gggctagaaa
    21901 agcttcggga caggctgtca tgccgatgat ggacatgccc cattcgcgga acaaatttga
    21961 ctcagctttt gttgaaaagc gaggaccctc aatggtgata tagacgccgg aatcatgtac
    22021 aacgcttccc gtttgcctga cggcgtctaa cacgttggtg cgcaatccac tgcaataagg
    22081 atcagcgctg cctatgtggg caaccattcc atcaccaaag aaggaatatg tgcgcctttt
    22141 ggtgaagtcg aataattgat ttggaatgac gaaatgactg ggtgcgtagt cttcacgtag
    22201 tgaaccgcag gcagaaatac tcacgatgcg cttggcacca atctttttta aagcgaaaat
    22261 gttggcacga taattgacct cggtgggtgt aaggacgtgt ccgaatccat gccgtgcaag
    22321 aaaagctaca ggtttctcgt gcaagattcc catcactatg ggtgaggaag gttttccaaa
    22381 gggcgtatcg acctctatgg ttttgacgtt ttttaaagcc ggaaactgat atatgccaga
    22441 gccaccgatc accgccaaaa aaggtgtagt gttcattgtt tattttcctt ttgttttaat
    22501 gaggatcgag gtttcttttt tgtgtctttt tcatggcgca tgtctttcca gagaattgaa
    22561 agcgcaaata cagtaaaaga aaccaagcca acggccatga gcccccgaag aaacaacaca
    22621 agtacaccac tgaattccat gtactcctcc aaacaatgta ctattgtacc cgacatttat
    22681 ccttttagtt cggctgggta taatggggtt aaccttatga aacagaaaca gggttttaaa
    22741 gagattgagc acacagccga tgtggctatg catgtatggg cagaaacact gcccgcattg
    22801 ttccaccagg cagcccttgg attgtatgcg ttggcggaaa tagaaatgca agaggcaccg
    22861 gtcgtttccc gctcgctttg tctggaagcg caagatgtgg aaacattgct cgtgaatttc
    22921 ctatctgagc ttctctttca acttgacaga ggagcagtgc ttcaggtcca acttctggag
    22981 gttgatggga ataccctgac gggaattttt atggagaaaa agatcacaca gattcacagg
    23041 gagatcaagg ctgttactta tcatcgtttg catgttgtta aagagaactc tatttttcgg
    23101 acagacattg tgctggatgt atgagaggcg agatggttaa gcttaatgat gtgcggcgaa
    23161 tatcagattt cgaatgggtc attcccaaat cgttgcggtc aggtatgaaa actgatgtgc
    23221 gcatcatcgc atccagagct attttggaaa aaagcctaaa cgatctggcg ctggagcagg
    23281 cgatcaatgc tgcgttcttg cccggtctgg tgggagaagt gctggtgatg ccggatgtcc
    23341 atcaaggata cgggtttccc attggagggg tggcagccac tgatgcagaa aaaggtgtca
    23401 tttcgcccgg tgcgattggt tacgatatca attgcggagt gcgtttgctg gcatcgcaag
    23461 ttgagttcca ggaggcgcgc cctttgatca atgaattatt gcagggcttg tatgatgccg
    23521 tgcctagtgg attgggacgg ggtggtttgc cgaggctgca gaaaacacaa ttggaaaagc
    23581 tcatgcatga aggggcgggt tgggcaaaac aagtcggttt tgctgaagat tctgacctgg
    23641 aactgaccga agaccaggga aaaatgaacg gcgcagatcc atctaaagtc agcgcacgag
    23701 ctgtggaacg aggggcggaa caggtcgggt cgctgggggc aggcaaccat tttatcgaag
    23761 tcagcatggt agaagacgtg tatgacccgg aatctgcaag ggtcatgggt ttggaaaaaa
    23821 gttgtttgtg tttgatgata cattccggct cgagaggatt agggcatcag gtttgcagcg
    23881 attatgtgca agagtttcag aaagctcagc gacaatatgc ctataattta cctgatcgag
    23941 aactggcttg cgcacctttg ctctccaaag aagggcaggc atatctggca gccatgcgct
    24001 gtgcagcgaa ttatgcattc tgcaaccggc aggtgatgtc acatttggct cgccaaacct
    24061 ttgaacggat attagccgcg cgatgtaaaa gaccgtatct gcggatggtt tatgatctgg
    24121 cacataatat cgggaagatc gaagaacatt gggttgagaa agagaaaatg caggtctgtg
    24181 tgcatcgcaa aggagccact cgtgccttcg gacccggggt gccagaagtc cctataagat
    24241 atcggcagat cgggcaacct gtacttatcc ctggcagtat gggtaccttt tcgtgggtgt
    24301 tgactggaaa atcagaagcg atgctgaaaa catttggctc ttcttgccat ggggcaggca
    24361 gggttttcag tcgtaaaggt gctcgaaaac tcatgcgagg gagtcaattg attcaggact
    24421 tgcaccgaag gggaatcgca gtaatggctg gatcgttagc cgggctggtg gaggaggcgc
    24481 cggaggttta taaagatgtc gatgaagtgg tcgaatcggt tgtacaggcg ggaattgtga
    24541 agaaggtggt taagctgaga ccgctggcag taatcaaagg ataaagttta ttgacgaatt
    24601 aaccgattga aaggtataat ccctctgttg ctttgcagga ggtaaactgt ggaacgttat
    24661 tttcaaatcg ctctcatgat cacatcggtc gcattgatcg ccagtatcgt ggtgcaatct
    24721 aagggtgtcg gactcggcgg cttgaccggt gccgatctgg gttctaacat tacaacccgc
    24781 cgtggtcttg aaaagaccat gttcaagatt acgatcgttc tggctgtaat ttttgtcgca
    24841 ctggttttag ggacgatcgc tgtagcaaaa taatttcctc ttccgttctt tcaatgggct
    24901 actctgatgg aatgggagta gacccatttt tatgctagaa ttcttttccg atgaaaaata
    24961 tacgatggca gttgattatt atcctggtga ccgggttcgt tattggcatc ctactcatca
    25021 gccaacaaac cgggtttagc attacccgca ctgtatccaa tcgaggagga acctatacag
    25081 aagcgctaat cggcaatcta caaaggttaa atccggtctt ggattactat aattccgttg
    25141 accgtgatgt ggacagttta atcttcagtt cattgatcag ttatgatgaa aatgggcaac
    25201 cccaaccaga gttggcaacc aattggggaa tttcttatga tgggctctct tacaattttg
    25261 acctgaaccc agatgccacc tggcatgatg gcacaccagt gaccagcgca gatatcgcat
    25321 tcaccatcga tctcatgcga aatgcagatt cagtgcttcc ggaagacctg aaagcatttt
    25381 ggaataatgt tcaggtcatt cccctgggtg accaggctat ccaatttcgt ctaacggaag
    25441 ctttcacccc attcatggat tatctatctt ttggggttct cccccaacat ctgttaggtg
    25501 atctctctta tcaggaaatc ttgaattcct cattcaatct tgaaccggtt ggaagtggac
    25561 cgtatcagtt tgagcggttg ataacagaaa atggtttgat taagggtgtt gtgctctcgg
    25621 caaattccgc ttatttcggg gacgagccct taataagtca aatcgtcttc ttatattatg
    25681 cagatgctgt aagcgcatat gctgcataca cagaaggaac cgtgcagggc atcagccagg
    25741 taaccaggga tatcttgccg gatgtgttaa gcaatccgga catctctatt tattctacaa
    25801 ttgagccaca agtttccatg attctgctga acttaaataa caataaggtg aggttcctgc
    25861 aagatgaaaa tgtccgaaaa gcgttgcttc attccatcga tcgacgtcgc atgatcgatt
    25921 cggtcttatt gggtcaaggc gtaatttcga acattcccct cattccttct aattgggctt
    25981 attacgatgg gatacagaac tacgcatatg acacccaaaa agcagctcaa tactttgaag
    26041 aggctggttt tacactaaac tcaagtgccg agaatgcagt tttggaagac gaagatgggg
    26101 ctgccctgtc gatccagctt gcccacccag atgatgatac gcacaccgct atcgcccaaa
    26161 ccatccagtc agattggcag aagatggggg tgcaagttga attagtggca gttccttatg
    26221 acgtgttgat acttgactat ctagaacctc tgacatatca ggctgcactg gtcgacctga
    26281 actttgaacg ttccccagac cccgatccgt atccattctg ggataaatca cagcaaagca
    26341 acggacagaa ctactcgcag tgggagaata ctactgtcag tgaatatctt gaagaagcaa
    26401 gagtatccac cgatcaggaa gaacgtgcaa aactctatcg gaattttcag gtagtgttcc
    26461 aggatgaatt acccgctcta ccgctttatt acccggtttg caattatgcc gtggatgaag
    26521 gagtcagcgg catccgtttg ggttcttttt atgacacacc cgatcgtttc ttctcgatct
    26581 tgaattggta tctggtcgca aaggtcaaca accagtaaag ggaaatagct accatgaaat
    26641 ttgggaaagg ggatgacggg ttcacagatc tcttgggcgg catgcgcgtg cggaaagatg
    26701 accaatatat cgatttgatc ggtgaaatgg atgaactatc cgcctggata ggggtcgtcc
    26761 gaagtgtagt gcatgatgct gctacgaaag aaatgctgca aaacgtgcag gaacatcttt
    26821 ccatgatcat ggcgaaaatc agtatggggc accaggaaaa acatattgat tctgcagcag
    26881 tggattcgag cctgcaaaca gtagagcaat ggatccgttt gcttgaaaaa gagacaacgc
    26941 ttccacgtac cttcatccaa ccaggtgaga agcgcgatgg atcgctcatg aatatgctac
    27001 gtgtggtgac gcgcagggtg gaaaggagag ccatcgcaac cctgagctcc ggtgagcaag
    27061 ctcattcttc cgtgacggtt tacttgaaca ggctttccac tttgttctat ctgctgtggt
    27121 taaaaattga acacagcaca taaccctact tttccggacc ctcatgcttt tttcaccagt
    27181 ttttcatggc tggttgagat aacgatcaag ccataagttg tggaaacttt gcattttctg
    27241 cattgttttg ggttctttgg tttttcaccc aaagtggatc acagacaccg atcatctatc
    27301 cattgaatta tctaatgggg cactgataaa tcggaaagga ttcaccgagg tatgcaaaac
    27361 ccatgcgctg atagagatgt atggaagcct ggttatcgtg ctgggtgttc acggtgatct
    27421 gtcttatcca tggaacagaa aaatgccaga gcatattccg taccaatgct gcaccgatac
    27481 cacggttgcg agcatctgat gacactgcca ggcgggtgag atgggctcgg ctaaggtctg
    27541 ctgtgctgat ctgaaatcct actactttct cgtccagctc ggctacggtg gaatatgccg
    27601 attgctcatg agccagtgtg agggtggtgg ttgagaattt ccagaaggat gcgaaagaag
    27661 tatcgtcaat ttgggcgact ttgggcagat catccagctt catcttgcga attcgaacgt
    27721 ccttttcttt ttccgtcttt ggaaaggggg gatgctgcca tacaagaacg atcacttttt
    27781 gggctttttg ccatccattg gtctggagta aggtctccat ccagttttga taacagaggg
    27841 ctgcaaccag atcgcgtttt tcaaaggggt agctgtcggt aatcgcggag aacaattttc
    27901 tccaggtata tgtgtaatcg cattcgttac gaatgcaaaa aaaacgcacc cagtgcatat
    27961 ggggagggtc cgctgccaat gcaagcaagc cattgatctt ggctcctgta tgcaagattt
    28021 gtgagggtgg tcggcgtaac cattccatgg ggctgcgcca gtccaggtgt gcatgggtgc
    28081 gattttgacc tttgaggaag gctacgatag cttgagaatt aagaaagttt tcaaatgtac
    28141 ttactgattc aaccgcagag ctcattgttg ggtttgatcg ggttcctgtg ccgggtcttt
    28201 gggaagaagt tgtaccatca gttgaattgc ttcggctatg gcagcgaggt cctttggctg
    28261 catacttttg agtttctgtg ccaattcgag ataaggggga ttggctggat cggtcacaaa
    28321 ttcctgtatg tgagcgggca gtgaagaaaa agcgcgcagg ttttgctgcg atttttgcca
    28381 gattcccatc gcgctgttct gatcaaggaa tgcttccaga tcgatggaaa gggcgtctgc
    28441 caggctcaac aaatcatcca gagggatgcg ggtcgttcca gattcatagc gtttcaaccg
    28501 ggagcgttgg atcccacaac gcttggcaag gtccactaat gcgatattct ttctctctcg
    28561 ttcgatctgt agaagggtgc cgatgacatt attgcgtatt tcaattaaat gggcagtatt
    28621 cccggtttta acaagtagat cggtagattt gtggtcgctg agcaattcaa acaaaggaac
    28681 gttcaggaaa taggataaag tttccgtttc tggcagggtc gggtatatct ccccacattc
    28741 gatcttcttg tagtgagcgc tggtagagcc aatggcatgg gcgcaatctt tcatggattt
    28801 tccgctttgg aaacgagcct gggttagttt ttgagcgatc atctccaggt ttgcttgata
    28861 ggatcccata aagaacctct gtgctggttg agtatattgt attatagcgt ggttattcag
    28921 caaaatatgt tcttatctct tgtctcccaa catctttcgg aacggaactg aattatttta
    28981 agttaacgtt tcagcttcga tgagccaact gactttgggc tggaaaaatc ctagcacatg
    29041 aaatcagagc ttctccagac aaaagcgcca ttgatcatgg tagcgcaggg aaccaacgtt
    29101 tgcagggact gcggaggaat agcaaagggg tccacaggta aaatggtcaa atctgcccga
    29161 gagccaacgg caagttttcc atgaactgcc tgacctggaa gaagggtagc aggggtgcag
    29221 gtataagctt ctaaggcatg ctggaggctt aacgcttgtt gaggatgcca cggtcgatgg
    29281 gtacgcttgt tctgtctatt aactgcggca tgcaaaccca gaaatggatt gggtgtttca
    29341 accggtgcgt cggatccgaa gaccagggag acgccggctg catgaatgga attccatgca
    29401 taggcgtatt tgcaacgttc tccccagtat tggtcagcgg tttccatgtc agatatcaca
    29461 tgaaaaggct gcattgaagc aatcacatga agagctgcca tgcgttgcat atctgatggg
    29521 tgaatgatct gaacatgctc gatacgatga ggtatggcgg taggtgttgg cattcttgcc
    29581 atcccatcca aagcgcaacg aaccgcttta tcgccaatgg catgaatagc caggcagata
    29641 ccattcccaa ccgcttgttc tgcgatttcg ttaagctctt tgggggaaag gacgagcatt
    29701 ccagtattat cggatgcctc ataaggttca agcattgcgg cggtttgagg acccaacgcg
    29761 ccatccatga ataattttaa ccaaccaatg cgtaggttct catctcccgt gccgcttacc
    29821 aaactgttct gtatggcatc ggaaagatga tcaacgggaa tattttttac aacgcgcaag
    29881 ggaaattggt gttcctcttt catctttttg agcaatggat agattttcca ttcgtccata
    29941 tcgtgcacag cggtaatgcc aaaacgtatc agctcttctt gcccttgtat gagagcagct
    30001 cgcatttgac cttcatctgg tggtggaatt atcttttcaa ccaaacgtga tgcggattca
    30061 agtagaatgc cggtaggttc accctgagaa tccctcgcta tttttccacc ttctggatct
    30121 ggcgtatctt tgtggatgcc tgccagagcc aacgctgcgc tgtttaccca actggcatgc
    30181 agtgacttgg cagccaggta aatgggatga tgcttgctga tactgtctag ctcttctttg
    30241 gtgcctatgc cctcttccca aacattgtga ttccatccct gacctcttat ccagacgcca
    30301 aacggagcgt tttggattgc ggctgaaact ctgcgcaggc atgcctgctt gctttcggtc
    30361 tcacaatcaa tggaggtcag cttcaagccg tattgaagca aatgaatatg cgcgtcggtg
    30421 aaaccgggca gcatgcatct accatgaagg tcgaaaattt cactatcacc atgagccagg
    30481 ttcaaaacat cttcattttt tcccacagcc agcacaagac cgttgtcaat ggcaagcgct
    30541 tcccccctgg gacgctgggg atgcatcgta taaatgattc cactgtgaaa tatcaacatg
    30601 gatcatgaat ggagcaattg ctcgatcagg ttttccagtt cttcttcaga ataataaaag
    30661 aaggtgatcg atccaccgtg ttttccataa ttcatggaca ctttggtgcc atattgtgag
    30721 cgcaactgct tttcgatatg ctggatttcc ggtggattgt cttttttctg agggggaaga
    30781 gagcttttgt gttcaaccag tttacggacc agttcttcgg tttggcgaac gttcagcccg
    30841 ttgctcacga tgcttttcat ggcagcgctt tgtgcggctg gattttccag ccccagtaga
    30901 gcgcgcccgt gcccttcgct gatctttccg tcggagatgg ctttgatcac gtcttgcggg
    30961 agtttcaaca ggcgaacggt atttgtaacg gcggcgcggc ttttccccac tcgcagagcg
    31021 atctcttcgt gcgagagtcc gaattcatca gccaactgca ggtaagcttc ggcagcctcg
    31081 atcgctgaaa ggtcttcgcg ctgcaagttc tcgatcagag ccagctccaa ttgctgctga
    31141 ttggtcatct tttctctcac cagaaccggc actgaatcaa gacctgccag tctggcagcc
    31201 cgtaaacgtc gttccccggc gatgagaatg aaatcaccgg tgctgttttc gtcgttcgta
    31261 acgatcagag gctgcaggat tccatgttct ttaatggatg cacacagttc ttgcagttct
    31321 tcgtcgtgga tctctgcgcg gggttgatag gggttgggac tgatccgctc aatcggaata
    31381 taactgaccc cttccgcagt tggtgcactt gaaaaatcac cactggggat cagcgcatcc
    31441 agacctttcc ctaaaccttt tctctgtgcc ataactgttc ctcatgttct ctagtcatcg
    31501 aatgtagagt cttccacaac cggcaccggt tttccatctt gctgcaagat ctcacgcgcc
    31561 agcgctgcat atgcgcgcgc ggctgaggag gttggcgcat acaccgtgat cggtaatcca
    31621 taggaaggcg cctctgccaa gcggatgcta cgcggaatga tggtctggaa cacccggtct
    31681 gggaagaatt tatgcacttc ttgcaccacg tccatggcaa gcttggtcct gccgtcatac
    31741 atggtcagca gaacgccacg cacgttcaat tcgggataaa gagagcgttt tatgcgttgc
    31801 aaagtatcgg tcaattgccc aagcccttcc aatgccaggt attcgcattg gactgggatg
    31861 agaacgccat cttttgctgc cagcaatcca ttgatggtca ggatactcaa agaaggcggg
    31921 caatcgacca gaatataatc atagcgatca acgatcggat cgatcatttc tcgcaagcga
    31981 aaatcacgtt tttgaacatc catgagttcg acttcagcac ccgctaatgc cggagacgaa
    32041 ggaaggatgg agatcttgag gcgtgggttg tgcaggatac tcggaccaat gggagtcgct
    32101 ccgatcatga cctcataggt gccttttttg acggtcaatt tgtcgatgcc taaagaggat
    32161 gtggcattcg cctgtgggtc gatatctatg atcaacactc gctgatcgta atgcccaaga
    32221 aaagcgccca aattgatcgc ggaggttgtt ttgccaaccc cacctttttg gttaaccaat
    32281 gtgtatatct ttgccatcat atctcaattc tatttcatga cctcaaccat gcaatgccga
    32341 atatcttgcc tggtcggaat gctttccaat ccactgcggc ttatggagag cgaagccagt
    32401 tgcacggcta gttttgctga ctgccacgca tccccggtgg taaataagcg agaaaaatac
    32461 actgcggcat agatatcgcc cgccccggtt gcgtctacca ctgggaaatt gggagcggga
    32521 aaataccgcg catccccttt ccagtaaact ctcgctccct cctctgcttc ggtgacaacc
    32581 aggattttac acaatctagc catttcatca atgacctttt catccccttc gacatcttcg
    32641 aggctgatca caacagcttg cataaggggg agtacgctct ctgcccaatc ccaagtgacg
    32701 ggatggacca atccttcatc atcccaactg cgcatccacc cctgcggtgt ggcgcataag
    32761 aacgcatgct gtgcaaagga ttttaccgtt tccagtggta tttcctgtgc aaccgggccg
    32821 aagtggatga tatctgattg cctccattgc acagggatac aatcaggcaa gatactatct
    32881 gcctggctat aacaaaattg ttttctcttc ccatcctgca tgacgttctt aaattgtgtc
    32941 gctcttgcgg aagcacagcg ttggaaggta attttttcag gcgtaggaag gtggagctct
    33001 tggggataag cggttactac gcggacaatg tgacccagtg cggcagcggt tagaccggaa
    33061 taagcggcag tgccacccag ttgcactcct gaaggagtga gatcttgggt tatgtgacca
    33121 agaataagat aagaaatttc attagttgcc atgtaataag cattgctgtt cgaaaggttt
    33181 tttaagatct gtaaaattat agcattaagt accctcctgc atgattgttt aagatgatgc
    33241 gagagattgg gtcggatggg atttgatgta ctataatttc cgcagagctt attttgcata
    33301 ataaaacac
//
LOCUS       DAOSDS010000015        30162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_735_length_30162_cov_5.684426, whole genome shotgun sequence.
ACCESSION   DAOSDS010000015 DAOSDS010000000
VERSION     DAOSDS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 30162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30162
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_735_length_30162_cov_5.684426"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLL88_03285"
     CDS             complement(<1..663)
                     /locus_tag="PLL88_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HOJ00618.1"
                     /translation="MRLRRAFLYTPGDDPHKIEKACTLKADCVCVDLEDAVSPSKKEE
                     ARLIVAHTLGTYDFGKSERLVRINAPSTPLFMPDLRTIVDAIPDGIVIPKAEDANTLQ
                     YVDGFLEAFEAKKELPIGLIRLVAIIESARSFVNLKEICEATPRLTALIFGAEDLAVS
                     LGARRSISNQEVFYARSQMVLHAAAYGLDAIDLVCNNYRDADALRREALEGAQLGYCG
                     KQV"
     gene            786..874
                     /locus_tag="PLL88_03290"
     tRNA            786..874
                     /locus_tag="PLL88_03290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:820..822,aa:Ser,seq:tga)
     gene            893..984
                     /locus_tag="PLL88_03295"
     tRNA            893..984
                     /locus_tag="PLL88_03295"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:927..929,aa:Ser,seq:gct)
     gene            1081..1548
                     /locus_tag="PLL88_03300"
     CDS             1081..1548
                     /locus_tag="PLL88_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOJ00619.1"
                     /translation="MTLSEKSQLVQQALEKHHLSLQVVELDASTRTSQEAAHAVGCEI
                     GQICKSLVFRSADTPLLFLVSGKNQLNVARVSADLGFPLEKADAAFTKEKTGFAIGGI
                     PPVAHATPIKTYIDRTLLEYPEIWAAAGTPHAVFKLESRLLLTLTGGTLIDVI"
     gene            complement(1532..1927)
                     /locus_tag="PLL88_03305"
     CDS             complement(1532..1927)
                     /locus_tag="PLL88_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00620.1"
                     /translation="MVGAFIQSMLGDVGRALYAFYQTYSLYINGAVILYGLCVFFAHR
                     SFYAVLEAIKKDLKIDEKREMGKEKLAEVIRSTTFDWNSLSRAAWFPFIALPGKVAIH
                     FKNRANLEKIFSVENLLVLLKGSLIKSHQ"
     gene            complement(2024..3418)
                     /locus_tag="PLL88_03310"
     CDS             complement(2024..3418)
                     /locus_tag="PLL88_03310"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HOJ00621.1"
                     /translation="METFLTTLKAVIDSLGATVVLPIIIFILGLILGAKPGKAFRAGV
                     TIGIAFVGINLVTNLMWATLSGTAQAMVTNWNIQRDVVDVGWPSAAAIAFGTDVGLWV
                     IPIALIINIIFILINWTKTLNIDVWNFWHFAFLGSLVYMVTGNLTYGLIAAAIAAALA
                     LFLADWTAKAVQSFYKLPGISIPHLTSAPGVPFAIVTNWILDKIPGVKNWKADPDTIQ
                     KKLGVFGEPVILGLIIGLVLGILGYYNADQAVVKILQTGINLAAVMLLLPRMVQILME
                     GLIPISEAARDFMKKRASDREIYIGLDSAILIGHPAAISTALVLVPIAILLSIILPGN
                     RVLLFADLAVIPFVVALFAPLMRGNIFRMIIAGTLELIAGFYIATGMADFFTSAAVNA
                     GFAMPEGAAKITSIADGFIWTGLVFTKVVQWLGGIGGLAVLAALVLVLFFFYRKNPKT
                     WDVLAGAPKPESKK"
     gene            complement(3448..3735)
                     /locus_tag="PLL88_03315"
     CDS             complement(3448..3735)
                     /locus_tag="PLL88_03315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017359149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ00622.1"
                     /translation="MVGLKRVLVACGTAIATSTVVARAIEEALKERGIDITTRQCKAS
                     EVKSLASDADLIVTTTPVPEDLGVPVVHTLAFLTGIGKEDAIEQIVEILSK"
     gene            complement(3823..4284)
                     /locus_tag="PLL88_03320"
     CDS             complement(3823..4284)
                     /locus_tag="PLL88_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ00623.1"
                     /translation="MNEAILKLLEPRGVLLGADVADSTEIIRLLGNELYKTGYVKESF
                     IDAAIERESRLPTGLPLGGAFHAAIPHTDIEHVVKPGLALATLKKPVAFCNMAIPSET
                     VPVSLVFLLALEQPKAQIEMLQAIAGVLQTAQVVAALMNAKKFDDVVCALS"
     gene            complement(4281..5600)
                     /locus_tag="PLL88_03325"
     CDS             complement(4281..5600)
                     /locus_tag="PLL88_03325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOJ00624.1"
                     /translation="MAKEVIMPKFGFTQEESEILEWLKKEGESVEKGDPIATVSTDKL
                     SMEVEAPESGILSGVRYKVGDIVPVTKIIAFILQPGEALPDVKDSPEPAPPAEKSEAA
                     TQATVSGNATPVALRMMQDAGIAAQAIQGSGTDGKITKTDVEEYLAKQKTGVVSGKVA
                     ATPAARRVAAEGDVELASIQGSGPLGRIQEADVRKAISGMPQPTGIRASGEATRMPLK
                     GMRRIIAENMARSWSEAPHMTLQVDVDMSAAKTLRELSVRKVDGTTQKFTYTALLTKV
                     VAWALTRHAKLNSRLEGNEVVLLPYVHMGVAVAVSEGLIVPVIQNADQKSVYQISDEL
                     KSTAERARNNKLVPDDLEGGTFTISNLGMYGIDRFTAIINPPQSAILAVGNIVERFVP
                     DENKNPALRPIMTITASADHRIVDGAEVAEFLADVKKGLENPGTMIV"
     gene            complement(5612..6598)
                     /locus_tag="PLL88_03330"
     CDS             complement(5612..6598)
                     /locus_tag="PLL88_03330"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOJ00625.1"
                     /translation="MREITYREALYEALDQVMAKDERVFLIGEDIGIYGGAFGVSADL
                     QQKYGKERVIDTPISEAGIAGACVGAAITGMRPVGEIMFSDFVVLSMEQLVLQAAKIR
                     FMFGGKASVPMVLRLPGGSGTGASGQHSGSYENWFVHVPGLKVVMPSTPYDAKGLLLA
                     SIEDNNPVMFFEHKLLYKVKGAVPEEMYTIPLGKSNVVREGKDLTIVATSIEVMRSLE
                     AAEQLAQEGIEVEVIDPRTLNPLDLEPITQSVIKTGKALVVHEAAKTGGFGGELVARI
                     MESEAFAYLDAPVRRLAGLDTPIPYNRNLEYHTVPQVENIVEEARKIVQGVY"
     gene            complement(6591..7562)
                     /locus_tag="PLL88_03335"
     CDS             complement(6591..7562)
                     /locus_tag="PLL88_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOJ00626.1"
                     /translation="MEKEKLIGGVFTPDEARRLLYLMMLTRAFEENAEELYALGKVHG
                     TMHLSIGQEAIAAGASSALTRNDYLLNTHRGHGHFLAWGGDLNRMMAEFLGKETGYCR
                     GRGGSMHIADVDANNLGANGVVAGNLPIAVGVGLSIKTRKTDQVALTLFGDGAANEGA
                     FHESLNMASIWNLPVIYLCENNLYAMSTPFDVAFKIEEISTRASAYGIKGVTTDGNDV
                     VAVHKAVREAAQRARKGDGPTLIEAITYRWRGHSKSDRQAYRTRDEVKEWMEKDPIPR
                     FAKKIGVQEKEMEELRQRVKQDIQNAVAFAENSPEPDLNTILEGVYA"
     gene            complement(7583..8536)
                     /locus_tag="PLL88_03340"
     CDS             complement(7583..8536)
                     /locus_tag="PLL88_03340"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOJ00627.1"
                     /translation="MKSDSRRHLLAEIASMYYLERRSQNEIALEFGYSRSAISRLLTE
                     AELSGVVEHKINFPSIRNVELEEKLINRYQLRNAIVVNRGKLSYTQTLHFVGEQGADV
                     FSKLTGDDQIISISTGTSIYEVVNALKPCQVTNVKIIQLLGAMGVKSDPSVDGPELAG
                     SLAKKVHGSYYALNAPMLMDSKNSRDMMLREKSIMSTVELAYKSDIVLAGVGCITEEP
                     HDSSVVRMGYMQAHEFIEIRKQGAVGYTCGWFFNDKGKIIEGQINDRVVAADFRRIRE
                     GKAVVLAIAAGERKAPAIKAALEGNLLDILITDSSAVEAFL"
     gene            8738..9559
                     /locus_tag="PLL88_03345"
     CDS             8738..9559
                     /locus_tag="PLL88_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOJ00628.1"
                     /translation="MLQSEVSLQELIVFIILSKFLPLFVYPLGLAIVLLWVSACLTRK
                     KRTARLLMVFAVLMLWACSTSPVATALARSLEWQYTYPHEVPQADAIVVLGGGTEPAI
                     SPRVNVELNSAGDRVFAAARLYQDGKAPLIILSGGNIDWMSDGASTPAGQMAEILAFM
                     GIPSSAMILEDTSKNTYENAVNTRAIVEEHGFDSLLLVTSAMHMPRSVKLFEKQGISV
                     IPIPVDYSVVENGEESRSLLDTLYGLLPNAGNLAVTTNALKEYIGIWTYSLQGDL"
     gene            9678..10433
                     /locus_tag="PLL88_03350"
     CDS             9678..10433
                     /locus_tag="PLL88_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOJ00629.1"
                     /translation="MIGSNKQPGDRRMAVLIDGDNAQPSLIEKILAEASKYGTVTIRR
                     TYGDWTTANMGGWKDSLQTYAIQPIQQFRYTVGKNATDSAMIIDAMDVLYGGSVDGFC
                     LVSSDSDYTRLATRIREKGFFVMGIGQKKTPRAFVNACDVFVYTENLLPIKQSAKQDG
                     QKKSAKKGQEPQTPDPVPLLKQAFDIAVQDDGWAFLGTLGHHMRQLDPGFDARTYGHN
                     QLSTLIRAYAKLFEIKEVKSPEGNSVIYVRLRD"
     gene            10456..10971
                     /locus_tag="PLL88_03355"
     CDS             10456..10971
                     /locus_tag="PLL88_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ00630.1"
                     /translation="MDPRLDIIYKRRSIRVFERREVEKEKLLELLQAGMAAPSASNGQ
                     PWEFVVVTDPEILAKLQGKLEHGKYNAPAAIAVLANLGITKKESSFRFWVQDCSAAVE
                     NILIAAAGLGLGSVWIGAYPKEDVTHALHEILGVPEDVHILNLIYVGYPAEEKPPRTQ
                     FEEARVHWQQY"
     gene            10990..11286
                     /locus_tag="PLL88_03360"
     CDS             10990..11286
                     /locus_tag="PLL88_03360"
                     /inference="COORDINATES: protein motif:HMM:NF025475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HOJ00631.1"
                     /translation="MDDFLHFRKLITPSLIKVLFWMSSLICIIAGIVLIASSFGTYGS
                     GAVSFLFGICIALLGPLFSRIICETLFVIFQNYQGAPASGLTGKTAKRGAQTKK"
     gene            11336..11989
                     /locus_tag="PLL88_03365"
     CDS             11336..11989
                     /locus_tag="PLL88_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOJ00632.1"
                     /translation="MFFIDYVDLAKLGIALLLGGVIGAERERYKKDAGLRTTILITLG
                     STLFTILSTKISLLSGSNADGTRIASSIVSGIGFLGAGVILQDRGRVKGLTTSAAIWI
                     SAAIGMACGAGEFLLAAACTLLTVIVLVVFSRFEDILEVSAEERTYEITSKISWEKYK
                     EIKNLFKSNGLIINKSKQEKKAGGDMVLTLEVSGPPKKHDKVVQKILSDKEIKEYWF"
     gene            12212..12565
                     /locus_tag="PLL88_03370"
     CDS             12212..12565
                     /locus_tag="PLL88_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOJ00633.1"
                     /translation="MLKIKRVYENTEESDGIRFLVERLWPRGIKKGELLMEAWLKDVA
                     PSANLRRWFAHDITKWDEFQKRYRAELDENPGAWKAILEALKQGNITLLYSARDKEHN
                     SAVVLKSFLEEYLRQ"
     gene            complement(12610..12735)
                     /locus_tag="PLL88_03375"
     CDS             complement(12610..12735)
                     /locus_tag="PLL88_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00634.1"
                     /translation="MNFIGTSWRNATLLVLAVMGLVAQATKPSGASANRVAGIET"
     gene            complement(12946..13401)
                     /locus_tag="PLL88_03380"
     CDS             complement(12946..13401)
                     /locus_tag="PLL88_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ00635.1"
                     /translation="MFSSDLINLSLEVNDKESAIEDLSNLLYANGYVKESFLSGVLER
                     EIKYPTGLKTISGGFAIPHTDTIHVEKTAVAVARLNRPVFFFEMGGAPENKVEVDLIF
                     MLAIHDPDDVMPILQRIIKIIQDQTITSQIKQCQSTIECAKILNESYAG"
     gene            complement(13404..14021)
                     /locus_tag="PLL88_03385"
     CDS             complement(13404..14021)
                     /locus_tag="PLL88_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ00636.1"
                     /translation="MEKQRELEKRIREEIIFTAKQMLKKGYVVGTAGNVSSVIRDRKT
                     IIITPSGAPYESLEPQDLIACDMDGNKISGEKKPSSEILMHANIYKNREDVGSIVHSH
                     SIYATALAVNGTPIPYCLDEMYFSVGDRSIPVAKYGKSGTQELADHIVEALGKENKAV
                     LVRNHGMVAVGKNMTEAFENCEVVEKTAMIFIFSSLTGKVQLLEK"
     gene            complement(14005..15075)
                     /locus_tag="PLL88_03390"
     CDS             complement(14005..15075)
                     /locus_tag="PLL88_03390"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ00637.1"
                     /translation="MKALVRSSAKRKDIRFCEYPKPELLDDCAIVEMKYAGICGFDID
                     SYNRDLKNDDHFSLPVIPGHEGSGIIHELHPHGTTFEVGQPVVFETTFSVCGQCSYCK
                     KGNVNLCPERTGIGSRANGCFAEFVLVPCRFLHKIPSGINLKIAALTEPLACAVHAVF
                     EIAKVEKNQTAIVFGPGTMGILIAILLNHIGVKVTVVGTPHSQHRLQLIQNQNIADVR
                     ILDSNDSPNENLSDAMFADVAFECSGNSSALISCLNCLKPLGKLIVVGDSKNPIQIDV
                     RKFLLNKELSFYGSKSSRSSSYTLALDLISPIYQRISTVISHIFPFEEWENAFAIAEN
                     RTGIKVLLNFNTDHRGQNGKTA"
     gene            complement(15096..16133)
                     /locus_tag="PLL88_03395"
     CDS             complement(15096..16133)
                     /locus_tag="PLL88_03395"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ00638.1"
                     /translation="MKAAIYRGIEKIEIVEIPMPECENDGVVIRVNACAICGSDIRTY
                     YKGTSYVKPNTIIGHEVVGTIAEVGKNVNHLKIGDRIAVGPIIPCGKCYFCQRGQQFL
                     CLHEEDFGGMHPGGFAEYMHIIGKAITHGSVCKVPEDMSSQEATLTEPLSSVYKAHEL
                     INTKSGDIVVVIGAGPIGCMHVEIAKIRGAKKIIQSEISTQRLNFAQKFGADVLVDSN
                     KTNLVEKVKEESENMGADIVICAAPSTEIVEEGLQLVKRGGTLLLFAGLPKDKPVVGL
                     NGNLVHYNDIHIVGTIGYSPRHFFESLALIHQKRVNPANYITGTISLDKIEHGFERVK
                     NGEELKLIVTP"
     gene            complement(16198..17478)
                     /locus_tag="PLL88_03400"
     CDS             complement(16198..17478)
                     /locus_tag="PLL88_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HOJ00639.1"
                     /translation="MEILKNIYDYVINAGAFVFVPIIIFLFGLIVGLKVTKAFRASLF
                     VGMGLAGIDLAIWSLLYPTFSTPASMMIERFGIKNLEYIDVGWGLPAAIGWSTPLVPI
                     VVGVIFLLNILLIVLNLTKTLDIDFWNYWHMTLMAAYVYFITDNVVISVILAAIYFII
                     LLKAADFFAPMVQKYWGYPGVSIPHGEVISMGIIQYPVNWILDKIPFLKNLKADQESI
                     RKRFGIFGEIPVMGFLIGVLLAILGGMAIKDIILTGVKVGLGFYLMPKMVSILVEGLL
                     PVSEGARDFVTKKFKNREIYLGLDAAVLMGDSSVLATGLLLLPYFILISTVLPGNGVL
                     PIGTLDATIWYVAIAVGLANGNIIKAFINGLITLPIILWVSTAMAPLLTKVAIAIQYQ
                     MPDLTSGALGVTGVTTGAEWPAWLLTLIAKIFAQ"
     gene            complement(17558..17851)
                     /locus_tag="PLL88_03405"
     CDS             complement(17558..17851)
                     /locus_tag="PLL88_03405"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ00640.1"
                     /translation="MIDSKFVLVSCGAGVATSTAVAVRIRELCEKEGIQVKISCCMAS
                     EIPAIVEAKSPDLIVATVQKPPDLAIPFFNGMPYLTGIGANDLDEKIINQLKN"
     gene            18003..19079
                     /locus_tag="PLL88_03410"
     CDS             18003..19079
                     /locus_tag="PLL88_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00641.1"
                     /translation="MNKPNQSGFNSDHDSHLESSRILSMWLERSGVKRKTILNEINRG
                     NWGDDSIGIKSFQNWTSYGDSARKISGPNTEITGKRVVAIINWFFAEHQHRANSIIKS
                     EELQRFVSIYDDIPITNQLQLHRILHDLEVELGERKIEIPVNSDWKKQFSNQNLFAYV
                     MDNYWTIRATSKYELALAGFNEEDMKDWGFWHRLSANTKGISKFSSHSPIRSLRGCYA
                     KTYYHNQLLRFLSDTKQLFIEKNPRYLSLLNLMQSFPDFEKIWNEVQRNNQQDNFSTS
                     IGYPVPFFMPDETLLWMMEFSSIISNQDNFRMIIWSPLNKETSQYLGLLSQSKIMNDY
                     SKSAFFIEDYAEYFTDEQKFALGI"
     gene            19341..19808
                     /locus_tag="PLL88_03415"
     CDS             19341..19808
                     /locus_tag="PLL88_03415"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOJ00642.1"
                     /translation="MKNKVQTLSLYQFLQRIPDESKMCEYIEKMRWAKGRYCPHCKSK
                     NTVEVKDEKPQPYRCKDCRKHFSVRTGTVMADSNLSLQKWLVAIYLMNTAKKGVPSCQ
                     VAKELGITQKTAWMLCHKTREAWTNPTSQFTGEVEIDETYIGGKEKNKHNSEN"
     gene            20257..21126
                     /locus_tag="PLL88_03420"
     CDS             20257..21126
                     /locus_tag="PLL88_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOJ00643.1"
                     /translation="MSRPVKNVIEPIKAPFEEVLAQIIGGEVQEEFIKKPKNYPRSPL
                     RYPGGKTRAINSIYSIIPENETKLCSPFLGGASIELACTTRMEVFGFDIFEPLIDFWQ
                     MLLDRREELVERVKSYYPLSRTKFYNLQKRYVHLQDKLERAAVFFVLNRSSFSGTTLS
                     GGMSPGHPRFTKSSIKRLEKFTAKNFSVECADFRDSIEKHEDAFLYLDPPYMNGQALY
                     GVKGDTHTGFEHHALADILYKRERWIMSYNDCEEIRTLYKGYPILSIEWVYGMSKEKK
                     SNEVLVLSKDMAL"
     gene            21123..22034
                     /locus_tag="PLL88_03425"
     CDS             21123..22034
                     /locus_tag="PLL88_03425"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HaeIII family restriction endonuclease"
                     /protein_id="HOJ00644.1"
                     /translation="MSFSQNDMGRAFEYGIVIAMSNIIPAKIVENTQLENSKRCFESC
                     SDIEKPKIVKASSEATAFLVAHDREFSDARGFTIQIQTDQMGGQGDVRDIIIRNQSTG
                     KETGISAKNRHFAIKHSRLSEQIDFGDEWFGVPCSDEYFQTIVPVFRELRTLHRQGEK
                     WRNIPDKKQRFYMPILQAFESEMRRLFSQDPQKSAHGLIEYLLGRHDYYKVIKENGQV
                     SILSFNIYNTLGWGSRLSLPGNLIQISLKPRSKTTLFMTFDKGWQISFRIHNASSVVE
                     PSLKFDIQPVGFPSTMSRNIIDYEIDD"
     gene            complement(22054..22263)
                     /locus_tag="PLL88_03430"
     CDS             complement(22054..22263)
                     /locus_tag="PLL88_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00645.1"
                     /translation="MQMLFVITNTPQKSPFEQEFRSIFLDHGSEVYGTAVNEPGDGSV
                     CALFVRSPNSTHMGPADFESGGCAI"
     gene            complement(22413..24794)
                     /locus_tag="PLL88_03435"
     CDS             complement(22413..24794)
                     /locus_tag="PLL88_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOJ00646.1"
                     /translation="MDTKTLTTLEYASILEQLEKYAAFSASQKLAHALRPTNDYPLAV
                     DRLVRTTEARRLLIEHAEISVGAARDIRTQVTLAARGGVLEIVDFIDIKGTLESAREL
                     GRFFGKQDQAYPQLCKVAAPLNPPAGLIEAISRVISEKGEVVDNASPQLASLRTQIRA
                     AHDRLQRKLDQIISDPNATRFLQEAIITQRNGRYVVPLRAEHKGRFKSVVQDQSASGS
                     TLFVEPLAVVELNNALFELQLAEKEEIRRILADLSLRVKEVAGELNEIVNALAVLDLS
                     LMCAKYAEALHATEPLFHHPNELVADRTRPILKLLKARHPLIDPQRVVPIDILLQDGV
                     FSLVITGPNTGGKTVTLKTAGLLSLMAQSGLHIPAQSGSEIMLFRDIYADIGDEQSIE
                     QSLSTFSGHITNIVRILKKARWNTLVLLDELGAGTDPQEGSALARAIMQNLIEKKVPC
                     LVATHYPELKAFAHTQPGVMNASMEFDLTSLQPTYHLILGLPGKSNALAIAGRLGMET
                     EIIELARTLLDPNELRTDDLLDEIHQQRELAREEKEIAAHELGEIQEIKLAWQKKMEG
                     IEEERLQILEKAQKDILDEIDQAREELKVIRKEASSKADSTETRKRIYKRLDQLEESR
                     RKPPEKNSFATPNAQPLKVGDRVYVQSLKVDGAVHAIAQDTVEVEIGKMRVKVSTADI
                     ARSKTKEKKVEREVPESGSTKLPDISMKSPGLECHLRGMRADDALAELERYLEKAYSA
                     GLPYVRIVHGKGTGAIRKVVQEYLNTSPYVERWELAMQSEGGEGVTVAHLRNV"
     gene            24886..25953
                     /gene="amrS"
                     /locus_tag="PLL88_03440"
     CDS             24886..25953
                     /gene="amrS"
                     /locus_tag="PLL88_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOJ00647.1"
                     /translation="MTNPGTLSEMLDPLTKPARFVEQLEEGRVRCYACAHRCTLKPGQ
                     RGICQMRFNQEGELRVPWGYVAAANVDPIEKKPFMHFLPGARAMTFGMLGCNFHCDFC
                     QNWISSQALRDPASNGAGSYVERISPAELVNLALRSGARVIASSYNEPLITSEWAVDI
                     FKVAREAHLYCAYVSNGFATPEILHALQPYLHGFKIDLKCMSDSHYRELGGSLQVVLD
                     SIALAHELGMWVEVVTLVIPDFNDSNEELWDTARALALISPDIPWHVTAFYPTYKRTH
                     NPPTSAEILQRAADIGQEAGLRYVYAGNLPGRVGSLENTYCPACNALLIERHGYRVSK
                     NSITSSAACPNCGTIIAGIWG"
     gene            complement(26323..26532)
                     /locus_tag="PLL88_03445"
     CDS             complement(26323..26532)
                     /locus_tag="PLL88_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00648.1"
                     /translation="MKLIILDQEAVPPLPKRRRTRLWMAECDQWNGRKYAEPIGEDHL
                     DIMKAKSQGANRVAPWRGQLGSCEL"
     gene            26798..27454
                     /locus_tag="PLL88_03450"
     CDS             26798..27454
                     /locus_tag="PLL88_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00649.1"
                     /translation="MNKSQTQTIFDSPFQVVKKVYARSGGMHFFFIALVLLSLEVFNF
                     STTKYALQDLLGEIGVGPTTWAVILASAFCGMNLIGIIRLFTPAAHQEQGAESWFLLG
                     AWILAAVMNTGLNWWGVSIAIYNHPVNSVLVVDPMAIVTVIPVFVVLMVMLLRVLIIG
                     NLAGTIRKATSASTKPQPTSNQPFGARPQTAQSNTGTLRRTIVPQGTHIFSASSKLDK
                     "
     gene            27525..28727
                     /locus_tag="PLL88_03455"
     CDS             27525..28727
                     /locus_tag="PLL88_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HOJ00650.1"
                     /translation="MGHPPRLIIFRAPTVPRLAYNPCMVNNEPLPHPIYIDTANALRS
                     LLQTLRSEKCIAVDTESDSLYVYHEKVCLIQFSTRAADYLVDPLSEIDVSGLGEVFAD
                     PDIEKVFHAAEYDIICLRRDYGFEFENIFDTMQAARILGLPKLGLSSLLEEYFQVTVD
                     KRFQKANWGKRPLPPAMADYARLDTHYLIPLHERLSLALQDANKMKIAREDFLRISRS
                     ICSENDKPLYTAVHGYHTLSPQQLAVLAQLCHYRERVAMQADLPPFKILSSSVLLDIA
                     RSDISSITDLSSISGLSEKLLHRHARGLLDAIQQGRSDPPIHLDRRPKPSDAYIARLE
                     RLKKWRQHLAREKAVESDVILPREIMEEIAKQHPSSPQQLEALMRDVPERFQQHQHAI
                     MHILKEHS"
     gene            28724..30124
                     /locus_tag="PLL88_03460"
     CDS             28724..30124
                     /locus_tag="PLL88_03460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ00651.1"
                     /translation="MKIHFNGAAQTVTGSQHLLEINGKKLLLECGIYQGKRAEFYSRN
                     CRFPFELAGLDAVILSHAHIDHSGNLPNLVKQGYSAPIYATPPTADLADIMLQDSGHI
                     YEEDARYLNQKKSRRGLPPVQPLYTKEDAQRVNPLFQRVGYGQVFEPIPGVQAHLLDA
                     GHMLGSAGVCLDIKEHGKQTRLWFSGDIGRPGLALIRDPQLPENAEHLIMECTYGDKS
                     HSDPEEAFMELRDVIQRTVARKGKIIIPAFSVGRTQEIVYNLNRMIREGDLPSMPVFV
                     DSPLAINATEIFRKHPDYFDDETHAFMQTGRHPALTFPGLEYTRSVEESKAINEKSAP
                     LIIIAASGMAEAGRILHHLKNNIEDERNTIVIVSWQAPNTLGRRLADREPLVRIFGEE
                     YRLRAEVATIGGFSSHAGQNFLLKYAQATHASLKGLYLVHGDPGAAAVFQQVLAEHQF
                     KSVLYPPLGASVNKFR"
BASE COUNT         7558 a         7790 c         6880 g         7934 t
ORIGIN      
        1 gacctgcttt ccgcagtatc ccagttgtgc cccttccagc gcttcgcggc ggagcgcatc
       61 cgcatcccgg tagttgttgc acaccaggtc gatggcgtcc aatccgtatg cggctgcgtg
      121 cagcaccatc tggctgcgcg cgtagaaaac ctcctggttc gaaatgctgc gccgcgctcc
      181 caaactcact gccaggtctt ccgctccaaa gatgagcgcc gtcaggcggg gggtcgcttc
      241 gcagatctct ttcaagttca caaaactgcg cgcgctctcg atgatggcaa ccaaacggat
      301 cagcccgatc ggcagttctt tttttgcttc aaacgcttcc aaaaagccat ccacatactg
      361 cagcgtgttg gcatcttccg ctttggggat aacgatacca tccgggatgg catccacgat
      421 cgtgcgcagg tcaggcatga aaagcggggt agaaggggcg ttgatgcgca ccaatcgttc
      481 cgattttccg aaatcgtagg tgcccagtgt gtgcgccaca atcagacgcg cttcttcctt
      541 tttggaaggg gaaactgcat cttccagatc aacgcacacg cagtccgctt tcaatgtgca
      601 ggctttttcg atcttatgcg ggtcatcccc cggggtatac aagaacgctc gtcgcaggcg
      661 catggctcct cgctttcctt tcaccccata ttatagtaag tattgcacgc tgtgcacatt
      721 cgcgccggat gttgtgtcgc ttcgccaagt atgatagaat tttgccgcac tgtactgtac
      781 aagttggagg gatggccgag tggtttaagg cgcctgtctt gaaaacaggt gtggggtgaa
      841 cgctccaccg tgggttcgaa tccctctccc tccgctggac gtgcaccgat ttggggaggt
      901 gccagagtgg ctgaatggga ccgcctgcta agtggttgtc ggggattaaa acctcgaccg
      961 agggttcgaa tccctccctc cccgctgttt ctcacgcttt atccaccaga gcaacgccgc
     1021 aattgcatag cgcattctct tttttaatac aggataaagc atcacagccg tggaggaagc
     1081 atgacccttt cagaaaaatc tcaattggtg cagcaagcac tggaaaaaca tcacctttcc
     1141 ctgcaggtcg tggagctgga tgccagcacg cgcacatcac aagaagcggc acatgccgtg
     1201 ggttgcgaga tcgggcagat ctgcaaatcg ctggtctttc gctctgccga tacccccctg
     1261 ctcttcctgg tcagcggtaa gaatcagttg aacgtggccc gcgtctccgc cgatctggga
     1321 tttccgcttg aaaaagccga tgccgccttc accaaagaaa agaccggctt tgccatcggc
     1381 ggcattccac ccgtggcaca tgccaccccc atcaaaacgt atatcgaccg taccttgctg
     1441 gaatatcccg agatctgggc agccgccggc accccccacg ccgtattcaa gttagaaagc
     1501 cgcctgcttc tcacgctcac cggcggcact ctcattgatg tgatttgatc aaagacccct
     1561 ttaatagaac cagcaggttc tcgaccgaaa agatcttctc cagattcgcc ctgttcttga
     1621 aatggatcgc gaccttgccg gggagcgcaa tgaatggaaa ccaggctgcc cggctgaggg
     1681 aattccaatc aaaggtggta ctgcggatga cctccgccag cttttccttg cccatttccc
     1741 tcttttcatc gatcttcagg tcctttttga tcgcctccag caccgcgtaa aaactgcggt
     1801 gcgcaaaaaa cacgcataag ccgtacagga tgacggctcc gttgatatag agactgtacg
     1861 tttggtaaaa ggcatacaac gcgcgaccaa catcacccag catgctttga ataaaagcgc
     1921 ccaccatgct tccacctctt gttgaaaaga tggggcggtc ccaaaagacc gccccaatgc
     1981 gtcattcagt acttcggtac aagggatgct acctacgttt tacctatttc ttgctctctg
     2041 gcttcggtgc gccagccagt acgtcccagg tcttggggtt ctttctgtaa aagaagaaca
     2101 acaccagcac gagcgctgcc agaacagcca aaccaccgat cccgcccagc cactgcacca
     2161 ctttggtgaa taccaatccg gtccagatga agccatcggc aatgctggtg attttcgcgg
     2221 caccttcggg catcgcgaac cctgcgttca ctgcggctga agtgaagaaa tcagccatgc
     2281 cggttgcaat gtagaatccc gcgatcagct ccagggtccc ggcaatgatc atgcgaaaga
     2341 tgtttccgcg catcaacggg gcaaacagag ccaccacaaa gggaatcaca gccaggtcgg
     2401 caaagagcag cacgcggttt ccgggcagaa tgatgctcaa aagaattgcg atgggaacca
     2461 ataccagcgc tgtcgaaatg gcagccgggt gtccgatcaa gatcgctgaa tccagcccaa
     2521 tgtagatctc gcggtcgctg gcgcgtttct tcatgaagtc gcgcgccgct tccgagatcg
     2581 ggatcaagcc ttccatcaaa atctgcacca tgcggggcag caacagcatg accgccgcca
     2641 ggttgatgcc cgtctgtaag atcttcacca ctgcctggtc agcattgtaa taaccgagga
     2701 tgcccagcac cagtccgatg atcagaccca agatcaccgg ttcaccgaac acgcccagct
     2761 tcttctggat ggtgtcggga tcggctttcc agttcttcac gccggggatc ttgtcgagga
     2821 tccagttggt tacaatggcg aagggcacac ccggcgcgga ggtgaggtga gggatggaaa
     2881 tgccgggcag tttgtagaaa ctttgcaccg ctttggctgt ccaatctgcc aggaacagtg
     2941 ccagagccgc cgcgatggct gccgcgatca gcccataggt aaggttgcct gtaaccatgt
     3001 aaaccagcga ccccagaaac gcgaagtgcc agaaattcca cacatcaatg ttcagggtct
     3061 ttgtccaatt gatcagtatg aagatgatat tgataatcag ggcgatcggg attacccata
     3121 gtcccacatc ggtgccgaag gcgatcgctg ccgccgatgg ccaacccacg tccaccacgt
     3181 cacgttggat gttccagttg gtgaccatag cctgggcggt gcccgataag gtagcccaca
     3241 tcaggttcgt caccaaattg atgccaacga acgcaattcc gatggtcacg cctgcgcgga
     3301 acgctttacc tggtttggcg cccaggatca atcccaaaat aaaaataata attggtagta
     3361 caacggtcgc ccccaacgaa tcgatcacag ctttcaaagt agtaaggaag gtttccatgc
     3421 taatacctcc tttttctttt attgctttta tttactcaaa atttcaacaa tttgttcgat
     3481 agcatcctcc tttccaattc ccgttagaaa tgccaaagta tgaacgacgg ggacacccag
     3541 gtcttcgggg acaggggttg tggtgacgat gaggtcagcg tcgcttgcca gcgatttcac
     3601 ctcagaagcc ttgcattggc gggtcgtgat atcgatgccg cgttctttga gagcttcttc
     3661 aattgcgcgc gctaccacgg tagaggtcgc aattgcggtt ccacacgcca ccagaactcg
     3721 tttcaagccg accatagttt gctccttcta ttatgagaga tgattatttc cattgtaaac
     3781 aggattgaaa aatatgcaat cttgtctttt gcttcttttc tctcaactca aggcacaaac
     3841 gacgtcatcg aatttcttcg cgttcattaa cgccgctacc acctgcgctg tctgaagcac
     3901 ccccgcgatt gcctgcagca tttcgatctg ggctttcggt tgctccagcg ccagcaagaa
     3961 caccaggctt accggtacgg tttcgcttgg gatagccatg ttgcaaaaag ccaccggttt
     4021 tttcaacgtt gccagcgcca gtccgggctt gacgacgtgt tcgatgtcag tgtgggggat
     4081 agctgcatga aaagcgccac ccaggggaag accggtgggt aaccggcttt cccgctcgat
     4141 ggctgcgtcg atgaagcttt cttttacata tcctgtttta tacagttcgt tccccagtaa
     4201 acgaatgatc tctgtcgaat ctgcaacgtc tgcacccaat aaaaccccgc ggggttccag
     4261 aagtttcagg atcgcttcat tcatacgatc atcgttcctg gattctccaa ccccttcttt
     4321 acgtctgcca gaaattccgc cacttccgcc ccatctacaa tccggtgatc cgcgctggca
     4381 gtgatggtca tgatcggacg cagagcggga ttcttgtttt catctggcac aaagcgctcg
     4441 acgatatttc ccaccgccag gatggctgat tgcggcgggt tgatgatggc agtaaagcgg
     4501 tcaatgccgt acatccccag attggaaatg gtgaacgtgc caccttccag gtcgtcgggc
     4561 accagtttat tgttgcgggc tctttctgcc gtgcttttca gttcatccga gatctgataa
     4621 acgctctttt gatcggcgtt ctggatgacc gggacgatca atccttcaga aactgccaca
     4681 gctaccccca tatgaacata aggcaaaagc acgacctcgt tcccttccag ccggctgttc
     4741 agtttagcat ggcgggtcaa tgcccaagct accactttgg tcagcagggc ggtataggtg
     4801 aatttttggg ttgttccatc caccttgcgc actgaaagtt cgcgcagggt ttttgcagcg
     4861 ctcatatcta cgtccacctg caacgtcata tgcggggctt cgctccaact gcgcgccatg
     4921 ttctcggcaa tgatgcggcg catacccttc aacggcatcc tggtcgcttc gccgcttgcg
     4981 cgtatgccgg tcggttgcgg catgcctgag atcgccttgc gcacatcggc ttcctgaatt
     5041 cttcccagcg gaccgctgcc ttgaatggat gcaagttcta catcgccctc cgctgcgact
     5101 cgccttgcag cgggggtcgc ggcaaccttt ccgcttacca cgccggtttt ttgtttggcg
     5161 agatattctt ccacatccgt cttggtgatc ttcccatccg ttccgctgcc ttgaatggct
     5221 tgtgccgcaa tgccggcatc ctgcatcatg cgcagagcca ccggcgtggc gttcccgctc
     5281 actgtcgctt gtgtggctgc ctcgctcttc tcggctggtg gagcaggttc aggcgaatct
     5341 ttcacatccg gcaacgcttc gcctggctgc aggatgaatg cgatgatctt ggtgaccggc
     5401 acaatatccc ccaccttata tctgacgccc gataagattc cgctctccgg cgcttccact
     5461 tccatgctca gtttatcggt agaaacagtc gcgatcggat cgcctttctc tacgctttca
     5521 ccttcttttt tcaaccattc aaggatctct gattcctcct gggtgaatcc gaatttcggc
     5581 atgatcactt ctttagccat ggtcgtacct cctaatatac gccctgaacg atcttgcgtg
     5641 cctcttccac gatattctca acttgcggta ccgtgtggta ttccaggttg cggttgtatg
     5701 gaatgggagt gtccaatcca gccaggcggc gcaccggggc atccagatag gcgaatgctt
     5761 cgctctccat gatccgggct accaattcgc cgccaaatcc accggtcttg gctgcctcat
     5821 gcactaccag cgctttcccg gtcttgatca ccgattgcgt gatgggttcc aggtccagcg
     5881 gattgagcgt gcgtggatca atcacttcca cttcaatccc ctcctgtgcc agttgctcag
     5941 ccgcttccag agagcgcatg acttcaatgg aggtggcgac gatggtcagg tcttttccct
     6001 cgcgtaccac gttgcttttg cccaatggga tggtgtacat ctcttcgggt accgctccct
     6061 ttactttata taataactta tgttcgaaga acatgaccgg gttgttgtct tcgatggaag
     6121 ccagcaataa acccttggcg tcgtaggggg tgctgggcat gaccaccttc aatccgggta
     6181 catgcacgaa ccagttctca taactgcccg aatgttgccc tgaagcgccg gttcccgaac
     6241 cgcccggcag ccgcagcacc atcggcacgc tggcttttcc gccaaacatg aagcggatct
     6301 tggcagcttg cagcacgagc tgttccatgg aaagcaccac aaaatcgctg aacatgattt
     6361 cccccaccgg gcgcatgccg gtaatcgctg ctcccacgca ggcgcctgca atcccggctt
     6421 cagaaatggg tgtgtcgatc acgcgctctt tgccgtattt ttgttgcagg tcagccgata
     6481 cgccaaaagc gccgccgtat atgccaatat cctctccgat cagaaaaacc cgctcatcct
     6541 tcgccattac ctggtcaagc gcttcataca gcgcctctcg ataggtgatc tcacgcataa
     6601 acgccctcca aaatagtgtt aaggtcaggc tccgggctgt tttctgcgaa tgctacagca
     6661 ttctggatgt cctgtttcac tcgttgtcgt aattcttcca tttccttttc ttgcacaccg
     6721 atttttttcg caaatcgcgg gatcggatcc ttttccatcc attccttcac ttcatcgcgt
     6781 gtgcggtaag cctggcggtc gctcttggaa tgtccgcgcc agcggtaagt gatggcttcg
     6841 atcagggtcg ggccatcccc tttgcgcgcc cgttgagccg cttcgcgaac ggctttatgc
     6901 actgccacca catcattgcc gtcagtggtc acccccttga tgccataagc gctggcgcgc
     6961 gtgctgatct cttcgatctt gaatgccacg tcaaaagggg tcgacatagc gtacaggttg
     7021 ttttcgcaca ggtagatgac cggcagattc cagatacttg ccatgttcag tgattcatgg
     7081 aaagcgcctt cattagccgc cccgtcgcca aataaggtca aagccacctg atctgttttg
     7141 cgggtcttga tgcttaaccc cacccccacc gcgattggca ggttgcccgc caccacgccg
     7201 ttggcaccca agttgttcgc atccacgtcc gcgatatgca tcgatccacc cctaccgcgg
     7261 cagtatcccg tttcttttcc aaggaattct gccatcatgc ggttcaggtc accgccccac
     7321 gccaaaaagt gtccgtgtcc gcggtgggtg ttcagcaaat aatcgttgcg ggtcaaggcg
     7381 ctgcttgccc cggcggcgat cgcctcttgc ccaatggaaa ggtgcatcgt tccatgcact
     7441 tttccaagcg cgtacagctc ttctgcgttt tcctcgaacg cccgtgtcag catcatcaag
     7501 taaagcaggc gtcgggcttc atcgggcgtg aacactcctc caataagctt ctctttttcc
     7561 atgtacatta acctccaaca atttataaaa atgcttcaac ggcggatgaa tctgttatta
     7621 aaatatccaa caggtttcct tccagcgcgg ctttgattgc gggcgctttc ctttcccctg
     7681 ccgctatcgc caacaccacc gcttttccct cacggatcct gcggaagtca gccgccacta
     7741 cgcggtcgtt gatctgcccc tcgatgatct ttcctttatc gttgaagaac cagccgcagg
     7801 tgtaccccac agcgccctgc ttgcgaattt cgatgaactc gtgcgcttgc atgtagccca
     7861 tgcgcacaac gctggaatcg tgcggttcct ccgtgataca ccccacgcct gccagcacaa
     7921 tgtccgattt ataagccagc tcgacggtgc tcatgatgga tttttcccgc agcatcatat
     7981 cccgcgaatt cttgctgtcc atcagcatgg gggcgttgag cgcgtagtag ctgccatgta
     8041 ccttttttgc cagcgatccc gccagttccg gtccatccac agaagggtcg ctcttcacac
     8101 ccatcgcccc cagcagttga atgattttta cgttggttac ctggcagggt tttaaagcgt
     8161 tcaccacctc gtagatggag gttcctgttg aaatggagat gatttgatca tcgccggtca
     8221 gcttgctgaa aacatctgcc ccctgttctc ccacaaagtg cagggtctgg gtatagctca
     8281 atttgcccct gttcaccacg atcgcattgc gcaattgata gcggttgatc aacttttctt
     8341 ccaattccac attgcgaata gaggggaaat tgattttatg ctcgaccact ccagaaagct
     8401 ccgcctcggt gagcaggcgg gaaattgccg aacgcgaata tccgaactcc agcgcgattt
     8461 cattttggct gcgtcgttcc agatagtaca tcgacgcgat ttccgccagc aggtgtcttc
     8521 gggaatcaga tttcatagca tggtccattg aatcattctc attaactata tcaccaaagt
     8581 gaatttttat caccattgtt aacagatatt cacttatgtg aaccatgctt tgcaggaacg
     8641 taaggggtag caacaaagta gccatcctcg ccctctccta gatagttccc tgattgtggg
     8701 gttatgagca catcgccgct gtcgtccctt gagtcccatg ttacaatcgg aggtcagttt
     8761 acaggagttg atcgtgttca tcattctgtc aaaattctta ccgctctttg tgtacccctt
     8821 gggactggcg atcgtccttt tatgggtgtc agcttgcctg acccgtaaaa agcgcacagc
     8881 caggctcttg atggttttcg ccgtcctcat gctgtgggct tgctctactt cccccgtcgc
     8941 tacggctctg gcgcgttcgc tcgaatggca gtacacctat ccccatgaag ttcctcaggc
     9001 tgatgcgatt gtcgtgctgg gtggcggcac cgaacccgct atctcgccca gagtgaacgt
     9061 tgaattgaac agtgctggcg atcgggtgtt cgccgccgcc cgtctatatc aggatggcaa
     9121 agcccctctc atcatcctca gcggtggaaa tatcgattgg atgagcgatg gcgcttcaac
     9181 gcccgccggt cagatggcgg agattcttgc gttcatgggc attccttcct ctgctatgat
     9241 cctggaagat acatcgaaaa acacttatga gaacgcagtg aacaccaggg cgatcgtcga
     9301 agagcatggc ttcgatagct tgctgctggt cacgtcagcc atgcacatgc cgcgttcggt
     9361 gaaattgttc gaaaaacagg ggatttctgt gatccccata ccggtggatt attcggtggt
     9421 cgaaaatggg gaggaaagcc gctccctgct ggacactttg tatggattat tgcccaatgc
     9481 cggcaacctg gcagttacga ccaatgcgct gaaagagtat atcggcattt ggacctattc
     9541 gttgcagggc gatctttaat ttctaagccg tcacggctct aacagtgctt ctttggagag
     9601 catccgctgc tgttctcccc tttatttcat tgctataatg caagtagaat cataaattaa
     9661 gttaaggaaa tattcgtatg attggatcga acaaacaacc cggagatcgc cggatggcgg
     9721 tcttgatcga cggggataac gcccagcctt ctctgatcga aaagatcctg gcagaagcca
     9781 gcaaatatgg cactgtaacc attcgccgca cctatggcga ttggaccacc gccaatatgg
     9841 gcgggtggaa ggattctctg caaacctatg ccatccaacc cattcaacaa ttccgctaca
     9901 cggtcggcaa gaacgccacc gattctgcca tgatcatcga cgccatggat gttctttacg
     9961 gtggcagcgt ggatggcttt tgcctcgttt ccagcgacag cgactacact cgcctggcaa
    10021 cgcgcatccg tgaaaaaggc ttcttcgtga tgggcattgg gcaaaagaag accccccgtg
    10081 cctttgtcaa tgcctgcgat gtttttgtgt ataccgaaaa tttgctgccc atcaaacaat
    10141 ccgccaaaca ggatggtcaa aaaaagagcg ccaaaaaagg tcaagaaccg caaacccctg
    10201 acccggtgcc gcttctgaaa caagcttttg atattgccgt tcaggatgac ggttgggctt
    10261 ttctgggcac cctcggtcat cacatgcgtc agctcgatcc cggtttcgac gcgcgcacct
    10321 atgggcacaa ccagctctct acgctcattc gagcttatgc gaaactgttc gagatcaaag
    10381 aggtgaaatc tcccgaaggt aattcggtga tttacgttcg tttgcgagac tgaaaacaga
    10441 aaggaataat aaacaatgga cccacgatta gacatcattt acaaacgccg cagtatccgg
    10501 gtttttgaac gccgggaggt ggaaaaagag aaattgcttg agctcctgca agccggcatg
    10561 gctgctccca gcgcgagcaa cgggcagccg tgggaatttg tcgtcgtcac cgatcccgaa
    10621 attctggcga aactacaagg caagctggaa catggtaaat ataacgctcc ggctgccatt
    10681 gcggtgctcg ccaatttggg catcaccaaa aaggaatcct cattccgctt ttgggtgcaa
    10741 gattgcagcg ccgcggtcga aaacatcctg atcgctgccg ccggtttggg tttgggcagc
    10801 gtttggatcg gcgcctatcc gaaagaagat gtcacccatg cgctgcacga aatcttggga
    10861 gtgccagagg acgttcacat cctcaacctc atttatgtgg gctaccctgc cgaagaaaag
    10921 ccccccagaa ctcagtttga agaagcgcgg gttcattggc agcaatatta aaatatggag
    10981 gaaaagtgaa tggatgattt tcttcacttt cgcaagttga tcaccccttc cctcatcaaa
    11041 gtcttgtttt ggatgtccag ccttatctgc atcatcgcgg gcatcgtctt gatcgcctcc
    11101 agctttggaa cctatggcag cggtgccgtt tcttttctgt tcggcatttg tattgctttg
    11161 ttgggtcctc ttttttccag gatcatctgt gagaccctct ttgtgatttt tcaaaactac
    11221 cagggcgctc ctgcctcggg gttaactggc aagactgcaa aaagaggcgc tcaaaccaaa
    11281 aaataaacca ctcgctcgct ttgggctttc ttcatttatt taaggaggaa aatgaatgtt
    11341 tttcattgac tacgttgatc tggcaaagct gggcatcgcc ctgctcttgg ggggcgtcat
    11401 tggcgcggag cgcgaacgct ataaaaaaga tgccggtttg cgcaccacca tcctcatcac
    11461 gcttggttcg accctgttca ccattctgtc aaccaagatc agccttcttt caggcagcaa
    11521 cgcagatggg acgcgcattg cgtctagtat tgtgagcggc atcggttttt tgggtgccgg
    11581 cgtcattttg caggaccgcg ggcgcgtcaa agggctcacc acctcggctg ccatttggat
    11641 ctctgctgcc atcggtatgg catgtggcgc gggtgaattc ctgctcgccg cagcctgcac
    11701 cttgctcact gtgatcgtgc tggtcgtgtt ctccaggttc gaagatatcc tcgaagtttc
    11761 tgctgaagaa cgcacctacg agatcacctc gaagattagt tgggaaaagt acaaagaaat
    11821 caaaaacctc tttaagagca acggtctgat catcaacaaa tccaaacagg aaaagaaagc
    11881 cggcggggat atggttctca ccctcgaagt cagcggacca cccaagaaac atgataaggt
    11941 cgtgcaaaag atcctatcag ataaagagat caaagaatac tggttctaga gagtctttcg
    12001 atgaggggat gcctgataaa aaatcgttgc gcatactttc cgccccactc ttttcgcacc
    12061 gtccttcttg tctgtggata gggggacaat tatcggttaa ttctctacag acataaatgc
    12121 agcgggatgc tatggtggtt tgatctgttt tctcttgctg cttacgagcc tggcagaaaa
    12181 aagggctctt cccaaattga aaggagcagc catgctgaag ataaagcgtg tttacgagaa
    12241 cactgaagaa agtgatggaa ttcgcttttt ggttgaacgc ttatggccgc gtggcataaa
    12301 aaagggagaa ctacttatgg aagcctggct gaaagatgta gctcccagtg ccaatttaag
    12361 gcgctggttc gcgcacgaca taacgaaatg ggatgaattt cagaaacgtt atcgggctga
    12421 gttagatgaa aatccgggcg cttggaaggc catcctggaa gctctcaagc agggtaatat
    12481 caccttgctt tacagcgcac gcgacaaaga acacaacagt gcagttgtac tcaaatcctt
    12541 tttggaagag tacttgagac aataattcaa tccctcacat agaagcggga agagttatga
    12601 aaacccgagt caggtttcaa tgccagccac cctgttcgca gaagcgcctg aaggcttggt
    12661 tgcctgcgca accaggccca tgacagccaa caccagcagt gtcgcgtttc tccaagaggt
    12721 tcctataaag ttcatcttcc ttgatgtgtc tcatctattc tccagcgcag cgagcacttc
    12781 tccaaccaca ataccagtga aaaaatgcca ctgccaggca tgccagcgtc aacacaccta
    12841 cgaacgctaa cgaagagtcc ttttgtctgc gtcaaaaaaa cccgcacatt gttatcacag
    12901 ccaactcatt tgttctgata atgtatcaaa caagaagttg tagattcaac ccgcatagct
    12961 ttcgttcaga atttttgcac actcgattgt tgactgacat tgcttgattt gcgaagtgat
    13021 cgtctggtcc tgaattattt tgatgattct ttgtaatata ggcataacat cgtcaggatc
    13081 atgtattgcc aacataaaga tgagatcaac ttccacttta ttttctgggg caccccccat
    13141 ctcaaagaaa aatactggac gatttaatcg agccacagcc acagcagttt tttcaacatg
    13201 aattgtatcc gtgtgaggga tggcaaagcc accagatatt gtttttaatc cggtaggata
    13261 ttttatttcg cgttcaagaa ctccacttaa aaaactttct ttcacatatc catttgcata
    13321 aagcaaattg gaaagatctt caatggcaga ttctttatca tttacctcaa ggctaaggtt
    13381 aattaagtca gaagaaaaca taattatttc tccaaaagtt ggaccttgcc tgtgagagaa
    13441 gaaaaaatga atatcattgc tgttttttct acaacctcac aattttcaaa agcttctgtc
    13501 atatttttac ccactgctac catgccgtga ttcctgacca agaccgcttt gttttctttc
    13561 cctaatgctt caacgatatg atctgccaat tcttgtgtcc cacttttccc atactttgca
    13621 actggaatgg atctgtcccc aacagaaaaa tacatctcat ccagacagta aggtataggt
    13681 gtcccattta cagccagcgc agtagcataa attgaatggg aatgaacaat agaacccacg
    13741 tcttctcgat ttttataaat gttcgcatgc ataagaattt cgctcgacgg ttttttttca
    13801 ccgctgatct tatttccatc catatcgcat gcgattaggt cttgtggctc cagtgactca
    13861 tagggagcgc cgcttggagt gatgatgatg gtttttcgat ctcgaattac cgaactgaca
    13921 ttgccagcag ttccaaccac gtatcctttt ttcaacattt gttttgcagt aaaaattatt
    13981 tcttctcgaa ttcttttttc taattcacgc tgtttttcca ttctgtcctc tatgatctgt
    14041 attgaaattt aatagaactt tgattccggt tctattttct gcaatagcaa aagcgttttc
    14101 ccattcttcg aaaggaaaaa tatgggagat aacggtgctg attcgttgat aaattgggga
    14161 aatcaggtca agtgctaaag tataggatga tgatcgacta gatttggatc catagaaact
    14221 caattcttta ttcaataaga attttctaac gtcgatttgt atcggatttt ttgaatcacc
    14281 aacaacaatt aatttcccga gcggttttaa acaattgaga caactgatca aagcagacga
    14341 attgcctgaa cattcaaagg caacatcagc aaacatggca tccgataggt tttcatttgg
    14401 ggaatcgtta ctgtcaagaa tccgaacatc agcgatattc tgattctgta tcagttgtaa
    14461 tctgtgttgg ctatggggtg tgccgaccac agtgacttta acaccaatgt gattcaataa
    14521 aattgctatg agaattccca tcgttccagg gccaaatacg attgcagtct gatttttctc
    14581 taccttagca atttcaaata ccgcgtgcac agcacatgcc aaaggttcgg ttaaagccgc
    14641 tatcttaaga ttgattcctg aaggaatctt atgcagaaat ctgcatggaa ccaagacaaa
    14701 ttcggcaaaa caaccattgg cgcgcgatcc aatccctgtt ctctcgggac atagattgac
    14761 attccccttt ttacagtacg aacattgtcc acatacggaa aaagtggttt caaaaacaac
    14821 cggttggccc acttcaaaag ttgtaccgtg tggatgaagt tcgtgaataa tacccgaacc
    14881 ctcatgacct ggtatgacag gtaaggaaaa gtgatcatca tttttcaaat cacgattgta
    14941 cgaatcgatg tcgaaaccgc aaatgcctgc atatttcatc tcaacaatag cacaatcgtc
    15001 aagcaactcg ggtttggggt attcacaaaa ccgaatatcc tttctttttg cactggaacg
    15061 aactaaagct ttcatatgtt ttacaggtag gttgttcaag gagttacgat gagttttagt
    15121 tcttcgccat tcttcactct ttcaaaccca tgttcaatct tatctaagga gattgttcca
    15181 gttatatagt ttgctggatt gacccttttt tgatggatca acgctaaaga ttcgaagaaa
    15241 tgtctgggag aataaccgat agtaccaacg atgtggatat cgttgtagtg aaccaaattt
    15301 ccgttcaatc ccactaccgg tttatctttt ggaagaccgg caaataataa cagagtcccg
    15361 cctcttttta caagctgcaa tccctcctcg acaatctctg ttgaaggagc tgcgcaaatt
    15421 acaatatctg cacccatatt ctccgattct tccttaactt tttcgaccaa attggttttg
    15481 ttcgagtcta caagcacatc cgccccaaat ttttgagcga aatttagtct ttgggttgat
    15541 atttctgatt gaattatttt ttttgcaccc cgaatttttg cgatttcaac atgcatgcat
    15601 cctataggtc ctgccccaat gacaaccaca atgtcaccgc ttttagtatt gatcaattca
    15661 tgcgctttgt acacagaaga taacggttct gttaaagttg cctcttgtga agacatatct
    15721 tctggaactt tgcataccga tccatgggta atagctttgc cgataatatg catatattcc
    15781 gcaaaaccac cggggtgcat tccaccaaaa tcctcttcat gcaagcacaa aaactgttga
    15841 cctctctgac aaaaataaca cttcccacag ggaatgatgg gtcctactgc aatgcgatca
    15901 ccaattttta gatgatttac atttttccca acttccgcaa tagtgccaac cacttcatgc
    15961 ccgatgatag tattcggttt gacgtaagag gtccctttgt aatatgttct gatatcagat
    16021 ccgcaaatag cacatgcatt gacgcgaatc actacgccat cattttcaca ttcgggcata
    16081 ggtatttcta cgatttctat tttttcgatt cctcggtaga ttgcagcttt cattcgtttt
    16141 acctctttgt ataatcacaa taaagggttg tttaataaaa caaccctttt tttcatccta
    16201 ctgtgcaaat atcttggcga ttaatgttaa taaccaagcc ggccattcag cccctgttgt
    16261 tacgccagtg actccgagtg ccccggatgt taaatcgggc atttggtatt ggattgcgat
    16321 ggctactttc gtcaataggg gtgccatcgc tgtagaaacc cagagtatga tcggtaaggt
    16381 gattaagcca ttaatgaagg ctttgataat atttccattt gccaatccga ccgctatagc
    16441 cacgtaccaa atggttgcgt caagggttcc aatgggtaat acgccatttc ctggaagaac
    16501 cgtactgatc agtatgaagt aaggaagaag gagcaaacca gttgccaaaa cagatgagtc
    16561 tcccatcaat acggctgcat ccaatcctaa ataaatctcc cggtttttaa atttcttggt
    16621 tacgaagtct ctagcacctt ccgaaaccgg cagcaaaccc tcaaccagga tggaaaccat
    16681 cttgggcatc aaatagaaac ccaaaccaac tttcacaccc gtcaggatga tatctttgat
    16741 cgccatcccg ccgagaatcg ccagcaaaac accgatcaga aatcccatca ccggtatttc
    16801 cccaaaaatc ccaaaccgct tcctgataga ttcttggtca gcctttaaat ttttcaagaa
    16861 tgggatcttg tctaaaatcc aattcacagg atattgaatg atccccatac tgattacttc
    16921 gccgtgtggg atacttaccc ccggatagcc ccaatatttt tgtaccatcg gggcaaagaa
    16981 atcggctgct ttcaatagga tgatgaaata gatggcagcc aaaattaccg agatgacgac
    17041 attgtctgta atgaaataga cataagcagc catgagtgtc atatgccaat agttccaaaa
    17101 atcgatgtca agggttttgg tcaaattcaa aacgatcaat agaatattaa gcaagaagat
    17161 cacacccaca acgattggaa caagcggcgt tgaccatccg atggccgcag gcaagcccca
    17221 tcccacatcg atatattcaa ggttctttat cccaaagcgt tcaatcatca tggatgctgg
    17281 tgtgctaaat gtaggataaa gcaggctcca tatagcgagg tcaatgcctg ctaatcccat
    17341 tcctacgaat aaacttgccc tgaacgcttt cgttactttt agcccaacaa tcaaaccaaa
    17401 aaggaaaata atgatgggta caaagacaaa agcgccggca tttatgacat aatcataaat
    17461 attttttaaa atctccattt tcttaccttt ccttttttca cttaaaagta ttatttgttt
    17521 gttcgattta gttatccatc ttcctccttt tctaaaatta attcttcaat tggttgatga
    17581 ttttttcatc caaatcatta gcgccaatac ctgtcaaata tggcattcca ttgaaaaatg
    17641 gaatcgctag atctgggggt ttttgtactg tggcaacaat aagatcagga gatttcgctt
    17701 caacaattgc cgggatctcc gacgccatac aacacgaaat ctttacctga attccttcct
    17761 tttcacataa ttcacgaatt ctcacagcta cagcagtgct ggtcgcaaca ccagcaccgc
    17821 aagaaactaa tacaaatttt gaatcgatca ttcattcacc tcgtgaattg tttattattc
    17881 ttgacgcgcg ataggatttc atataataat aaacaggata ggttccgtta ataatataaa
    17941 acgaacatcg agaaaccatc aattagtaaa atatctaacg cttagtggag ttttctctaa
    18001 cgatgaataa accaaaccaa tccggattta attcagatca tgatagtcat ctagaaagca
    18061 gcaggatcct ctcgatgtgg ttagaacgga gtggggtgaa gagaaaaacc atattgaatg
    18121 aaattaatag aggaaattgg ggagatgatt ccataggaat taaatctttt caaaattgga
    18181 cctcatacgg cgattctgcc agaaaaatta gtggtccaaa tacggaaatt acggggaaaa
    18241 gagtagtagc cattattaat tggtttttcg ctgaacatca acatcgtgcc aactctatca
    18301 taaagtcgga agaattgcag cgttttgttt caatctatga tgatatcccc attacgaacc
    18361 aattacagct ccaccgaatc ctgcatgatc tcgaagtgga attaggcgaa agaaagattg
    18421 aaatacccgt caattccgat tggaagaaac aattctctaa tcaaaatttg ttcgcttatg
    18481 tgatggataa ctactggact attcgagcta cttccaaata cgaattggcg ctagcgggtt
    18541 ttaacgaaga ggatatgaaa gattggggct tttggcatcg gctatcagcc aatacaaaag
    18601 gtatctccaa gttttcatct catagcccaa ttcgatcgtt gagaggttgt tatgccaaaa
    18661 catattacca taatcagcta ctgcgcttct tatctgacac aaaacaactc tttattgaga
    18721 agaatccacg gtatttatct cttttgaatt taatgcagtc ctttcctgac tttgaaaaaa
    18781 tttggaatga ggtacaacgc aataaccaac aagataattt ctcaacctca attggttatc
    18841 cagttccttt ttttatgcct gacgaaactc ttctttggat gatggaattc agctcaataa
    18901 tttctaatca ggacaatttt cgaatgatca tttggtctcc gttaaacaaa gaaaccagtc
    18961 aatatcttgg actgctttcc caatcgaaaa taatgaatga ttattcaaaa tcagcctttt
    19021 ttattgagga ctatgctgaa tacttcacgg atgaacaaaa attcgcattg gggatatgag
    19081 agcttctcaa taattaccga cttttcttaa ctcaataatc aaaatcatca taaaatgtga
    19141 atccaggttt ttatataggg tagttttggt aaaactttgc taaatagagc aaagttttac
    19201 cacaggaaat cgaaccttaa aaccattgat tcagaattgt ttttttcttt tttcatcaaa
    19261 atgcctgaga gtacaaatat gagcgtctga ttccataaat aattattgtg gaatattagc
    19321 cataagagaa tggatcagaa atgaaaaaca aagtacaaac acttagttta taccaatttc
    19381 tacaaagaat tcccgatgaa agcaaaatgt gtgaatatat tgaaaaaatg cggtgggcaa
    19441 agggtagata ttgcccacat tgcaagtcaa aaaacactgt ggaagttaag gatgaaaagc
    19501 ctcaacctta tcgttgtaag gattgtagaa aacattttag cgttcgcact ggtacggtaa
    19561 tggctgatag caacttatca ctgcaaaaat ggttagtagc tatttatctg atgaatactg
    19621 caaaaaaggg tgttcccagt tgtcaggtgg cgaaagagtt aggcattacc caaaaaacag
    19681 cctggatgtt gtgccataaa acccgcgaag cgtggacaaa cccaacatct caatttaccg
    19741 gagaagttga aattgacgaa acctatattg gcggtaagga aaagaataaa cataattccg
    19801 aaaactgaat gctgggcgtg acatagtagg aaaacaagga atactcgggt gcaggaaacg
    19861 gactggaaaa gtaaaaacag cggtcgcaaa aaataatggc agcgatgaaa cctatagttt
    19921 tgtgacaaaa aatatcagaa ttggagccac gatttacact gatgaacata aatcgtataa
    19981 tgatttatac agatacaatc gccaatcagt aaatcattca cgcaaggaat atgtaagagg
    20041 agagatacac acaaatggca tcgaatcatt ttgggctctt ctgaaacgcg gatattacga
    20101 aacatttcat cactttcctc gtgaacatgc tatgagatac gccaatgaat ttgcaacccg
    20161 cttaaatcaa aatggctgtg acacagaaga cgtactggca atcactataa aaaattcagt
    20221 agggaaaaac ttaccatata aggtattaac ccaaccatgt ctagaccagt aaaaaatgtt
    20281 attgagccta tcaaagcacc atttgaagaa gttttagcac agataattgg tggagaggtt
    20341 caagaagaat tcatcaagaa accaaagaat tatccgcgtt ctccattaag ataccctggc
    20401 ggaaagactc gcgcgataaa ttctatttat tcaatcattc cagaaaatga aactaaatta
    20461 tgttccccct ttcttggtgg agcgtctatt gaacttgcat gcactactcg catggaagtg
    20521 tttgggtttg atatttttga gccattaata gatttttggc aaatgctttt ggataggcga
    20581 gaagaacttg ttgagcgcgt gaaaagttac tatcctttat ctcgcactaa attctataat
    20641 ctgcaaaagc gatatgttca tttacaagat aaattggagc gtgcggctgt atttttcgtg
    20701 ttgaatcgtt cttcattttc tggcacaact ctatcaggtg ggatgtctcc cggacaccca
    20761 cgttttacta aatcatctat aaagcggttg gagaaattta cagcaaaaaa tttcagtgtt
    20821 gaatgtgcag attttaggga ttctatagaa aaacatgaag atgcttttct atatcttgat
    20881 cccccctata tgaatgggca agctctttac ggggtaaagg gagatacaca tacaggattt
    20941 gaacatcacg cgctagctga tattctttat aagcgcgaac gatggataat gtcatacaat
    21001 gattgtgaag aaataagaac gctatataag ggatatccaa ttctttcgat tgaatgggtc
    21061 tatggaatgt caaaggaaaa aaaatccaat gaagtacttg tattaagtaa ggatatggca
    21121 ttatgagttt ttcacaaaat gatatggggc gtgcatttga atatggtatt gtgattgcca
    21181 tgtcaaatat tattcctgcc aaaatagttg aaaatacgca attagaaaat tcaaaaagat
    21241 gttttgagtc atgctctgat atagaaaaac cgaaaatagt aaaagcttca tctgaagcaa
    21301 ccgcatttct ggttgcacat gatagagaat tttcggatgc cagaggattt acaattcaga
    21361 ttcaaaccga tcaaatgggt ggtcaaggag atgtgcgaga tataatcatt cgtaatcaat
    21421 ctacaggtaa agaaactggt atttcagcca aaaatagaca tttcgcaata aaacactcgc
    21481 gcctatcaga acaaatagat tttggagatg agtggtttgg agttccttgt tcggatgaat
    21541 attttcaaac aattgttccg gttttccgtg aattgcgaac attacatcga caaggggaaa
    21601 aatggcgaaa tattccagat aaaaaacaaa gattttatat gccgattttg caggcatttg
    21661 aatcagaaat gaggagatta ttctctcaag atccacagaa atctgcacat ggattaattg
    21721 aatacctctt aggaagacat gattactata aagtcattaa agaaaacggg caggtttcta
    21781 ttctttcttt caatatttac aatacgcttg gttggggaag cagattatca ttaccaggta
    21841 atctcataca gataagtcta aagccaagat caaaaacaac tttatttatg acatttgata
    21901 aagggtggca gatttctttt cgcattcata atgcaagttc tgtcgttgag ccatccctaa
    21961 aatttgacat tcagccagtt ggctttccaa gcacaatgtc acgcaatatt attgattatg
    22021 aaattgatga ctaacattgt atataaccct taattaaata gcgcaaccgc ctgattcgaa
    22081 atcagcgggt cccatatgag tggagttggg tgaccgcaca aagagtgcgc acacagaccc
    22141 atcaccaggt tcattgacgg cagttccata cacctcgctt ccatgatcca gaaaaatgga
    22201 acggaattct tgctcgaatg gacttttctg gggtgtgttg gtgatgacaa aaagcatttg
    22261 catattttca tactttgcag taatatagaa gcactgttcg gagggaatta tgctttttac
    22321 cttcgcacgc tcgtcgtagc acggaattgc aaggggtacc gtgcgagaaa ggtcgatagt
    22381 atctcgaggt cagctactat cgacctttta tgttaaacgt tccgcaaatg ggcaaccgtc
    22441 acgccttccc caccctcgct ctgcatggcg agttcccatc gttccacgta aggagaagtg
    22501 ttcagatatt cctgtaccac ctttcgaatc gcgccggtgc cctttccatg cacaatgcgc
    22561 acataaggca atcccgcaga atacgctttt tccaggtagc gttccaattc tgccagcgcg
    22621 tcatccgcgc gcatcccgcg caagtggcat tccaatccgg gtgacttcat ggagatatcc
    22681 ggcagctttg tagaaccgct ctctggcact tctcgctcca ctttcttttc cttcgtctta
    22741 gagcgggcaa tgtccgccgt gctcaccttg acgcgcatct ttccaatctc cacttccacc
    22801 gtgtcttgcg caatcgcgtg cacagcccca tctaccttta atgactgcac ataaacccga
    22861 tcgccaactt tcaacggctg cgcgtttggg gtcgcaaaac tgttcttttc gggcggtttg
    22921 cgccggctct cttccagttg atccagcctt ttatagatcc gtttgcgggt ctccgttgag
    22981 tccgccttcg agctcgcttc tttgcggata actttcagtt cctcccgtgc ctggtcaatc
    23041 tcgtctagga tatccttttg tgccttttcc aggatctgaa ggcgttcttc ttcgattcct
    23101 tccatttttt tctgccatgc cagcttgatc tcctggatct cgccaagttc atgggcagcg
    23161 atttcttttt cttcccgtgc cagttcccgc tgctggtgga tctcatccag caagtcatct
    23221 gtcctcaatt cattgggatc aagcagggta cgagccaatt cgatgatctc cgtttccatc
    23281 cccaatctgc cggcgattgc cagagcgttc gactttccgg gcagtcccaa gatcaagtga
    23341 taggttggct gcaggctggt cagatcgaat tccatcgaag cgttcatcac gcccggctgg
    23401 gtgtgggcaa acgctttcaa ttcaggataa tgggtagcga ccaggcaggg cactttcttc
    23461 tcgatcaggt tctgcatgat cgccctcgcc agagctgacc cttcttgagg gtccgtgccg
    23521 gcgcccagtt cgtccagcag caccagcgtg ttccaacgcg ctttttttaa tatgcgcaca
    23581 atattggtga tgtgccctga aaaagtggaa agcgactgtt cgatggattg ttcgtcaccg
    23641 atatccgcat agatgtcgcg gaacaacatg atttcagacc ccgattgggc agggatatgc
    23701 agtcccgatt gcgccatcag cgaaagcaga cccgcggttt ttaacgtcac cgtcttcccg
    23761 cccgtgttcg gaccggtgat caccagggaa aaaacgccgt cctgcagcaa gatgtcaatg
    23821 ggtaccaccc tttgcgggtc gatcaaggga tggcgggctt tcaacaactt aagaatgggt
    23881 ctggttcgat cggctaccag ttcgttggga tggtgaaaga gcggttccgt tgcatgtaac
    23941 gcttcagcat acttcgcaca catcagcgac aggtcaagta ccgccagcgc atttacgatt
    24001 tcgttcagtt ctcccgccac ttctttaacg cgcagcgaaa gatcagccag aatgcggcgg
    24061 atctcttcct tttccgcaag ctgcagttca aagagagcgt tgttcaattc caccaccgcc
    24121 agcggttcca caaagagcgt tgaaccggat gctgattgat cctgcaccac gctcttgaaa
    24181 cgtcctttat gctccgcgcg cagtggcacc acataccgcc cattgcgctg ggtgatgatg
    24241 gcttcctgca aaaaacgggt cgcgtttggg tcgctgatga tctggtccag tttgcgctgc
    24301 aagcggtcat gggctgccct gatttgtgtc cgcaggcttg ccagttgcgg agaggcgtta
    24361 tccactacct cgcccttctc tgaaatgacg cgactgatgg cttcgattaa ccctgccggc
    24421 ggattgaggg gagcagccac cttgcacaat tgagggtaag cctgatcttg cttgccgaaa
    24481 aaccgcccca gctcacgagc cgattcgagg gtgcctttga tatcgataaa gtcaactatt
    24541 tccaacactc ccccgcgtgc agccagcgtt acttgagtgc ggatatccct tgccgcgcca
    24601 acgctgatct ccgcatgctc gataagcagt ctgcgcgcct ctgtggtgcg caccaggcga
    24661 tccacagcca agggataatc attggtcggt cgcaatgcgt gcgctaactt ttgcgatgcg
    24721 ctgaatgccg cgtatttttc cagttgctca aggatgctcg catattccag ggtggtgagg
    24781 gttttcgtgt ccatttcttc attattataa tggggagagc ttacttcatt gtcttttctt
    24841 ccgatgagca ttttcgatgc gcactggctt gttataatac ggataatgac aaatccagga
    24901 acgctctcag aaatgctcga ccccttgacc aaaccggctc gtttcgtgga acagttggaa
    24961 gagggaagag ttcgctgtta cgcctgtgct caccgctgca cgctcaaacc cgggcagcgc
    25021 ggcatctgcc agatgcgctt caaccaggag ggggaactgc gcgttccctg gggttacgtg
    25081 gcagccgcca atgtggaccc catcgaaaaa aaacccttca tgcattttct ccccggcgcc
    25141 cgcgccatga cctttggcat gctgggctgc aacttccact gcgatttttg ccagaactgg
    25201 atcagttcgc aggcgctgcg cgacccggct tcgaatggag ctggtagtta cgtcgaaagg
    25261 atatctcccg ctgagctggt caatcttgcc ctgcgctccg gcgccagggt catcgccagt
    25321 tcctacaacg aaccgctcat taccagcgaa tgggcggtgg acattttcaa agtggcgcga
    25381 gaagcgcatt tgtactgtgc gtacgtgtcc aacggctttg ccacccctga gattctgcat
    25441 gcgctgcagc cctatctgca cggcttcaaa attgatctga aatgcatgtc cgacagccac
    25501 tatcgcgaac tgggtggcag cctgcaggtt gttctcgata gcattgccct ggcgcacgaa
    25561 ctgggcatgt gggtggaggt ggtcaccctg gtcatccccg atttcaacga tagcaatgag
    25621 gaattatggg ataccgccag ggctctcgcc ctcatttcac ccgatattcc ctggcacgtc
    25681 accgcttttt atcccaccta taagcgcacc cacaacccgc ccacctccgc agagatcttg
    25741 cagcgagcgg cagatattgg gcaagaagcg ggtctgcgct atgtgtatgc cggcaacctc
    25801 cccggcaggg tgggttccct ggagaacacc tactgccctg cgtgcaatgc gctgttgatc
    25861 gaacgccatg ggtacagagt ttctaaaaac agtatcacca gctccgctgc gtgtcccaat
    25921 tgtggcacga tcattgcagg gatttggggg taagaatttg cacagttgtg ccaggaacca
    25981 tttccgttaa cgtacgttaa tagagtacaa caaatgaatg aatgatctct cttgcgcgta
    26041 gcttttttgc gcaatggggg ttattcatta aaagacccaa ccaagtcgcc gcgccccttc
    26101 ccctttggga aaggacttgc ctgggagaag aagaggtgca tcatggctgt ttgtattatt
    26161 ttcgtcatcg cattggtcag tatcatgttc cttgccatga gcttgtcggg tctgcatgtt
    26221 tttaagaaat gatagaatca ttcgatcatc cctttgagaa gtgacaaaac gcgcgcggca
    26281 gtttatcatt aactcatagg aacgaaggat cacttgcagc gcttatagct cgcaggatcc
    26341 caactgacca cgccagggag cgacacgatt tgctccctgg ctctttgctt tcatgatatc
    26401 gagatgatct tcccctatcg gttcagcata tttccgccca ttccactggt cgcattctgc
    26461 catccaaagc cgggttcttc tccgcttggg taacggcgga accgcctcct ggtccaggat
    26521 aatgagcttc attgcccact gtccgaactg cttttgtgct gaaaaacagc agcgcctcag
    26581 tcacgatacc gggccgaaga ggctaacttt ttccacgttt tctccttcaa tcgtcgagtc
    26641 aatatgccca tatcttcttg aaagatgaga tttccagagt gccctcctat atctgggggt
    26701 tactgaattc aattcaccac ccctatggct tgacgacaat agaattcttg ttctataata
    26761 gaacatgtat tctaaatgat cttcaatgga ggaaatcatg aacaaaagcc agacacagac
    26821 cattttcgac agtcctttcc aggttgtgaa aaaagtatat gcacgatccg ggggcatgca
    26881 cttcttcttc attgcccttg ttttgctttc gctggaagtc ttcaatttca gcaccaccaa
    26941 atatgccctg caagatctgt tgggtgagat cggtgttggt cccactacct gggcggtgat
    27001 cctcgccagc gcattttgcg gtatgaacct catcggcatt atccgcctgt tcacccctgc
    27061 tgcccaccag gaacaggggg cggaaagctg gttcctgctt ggcgcctgga ttttagcagc
    27121 cgtgatgaac accgggctca actggtgggg cgtttccatt gccatctata accaccccgt
    27181 caacagcgta ttggtggtgg atcccatggc catcgtaacg gtcatccccg tctttgttgt
    27241 cctcatggtc atgctcttgc gcgtcttgat catcgggaat ttggctggca ctatccgcaa
    27301 agccacctcc gcttccacga aaccacagcc aaccagcaac caaccttttg gggctcgccc
    27361 gcaaaccgca caatcaaaca ctggcaccct gcgccgcact atcgttccgc agggcaccca
    27421 catcttctcc gcttcttcga agctcgacaa atagcgctct cctcgcatcg ctccttttca
    27481 tcacccacag catggaaact ccgccatgct gtgggtcttt aaatatgggg catccccctc
    27541 gattgattat cttccgcgcc ccaacagttc cccgcttggc ttataatccc tgtatggtca
    27601 acaacgaacc acttcctcat ccgatctaca ttgatacggc aaatgctttg cgctcgcttc
    27661 tgcaaactct tcgctcagaa aaatgcatcg ctgtggatac cgaatcggat agcttgtatg
    27721 tctatcacga aaaagtctgt ttgattcagt tttccacccg cgccgcagat tatctggttg
    27781 acccgctcag cgagatcgac gtttcaggat taggtgaagt tttcgcggac cctgacattg
    27841 aaaaggtttt tcacgccgcc gagtatgaca ttatctgctt gcgcagagac tatggttttg
    27901 aatttgagaa catcttcgat accatgcagg cagcccgtat cctggggctg cccaaattgg
    27961 gcttgtcttc cttgttagaa gagtacttcc aggtgacggt ggataaacgc ttccaaaaag
    28021 ccaactgggg caaacgccct ttgccgcccg ccatggccga ttatgcccgt cttgacaccc
    28081 attatctcat cccccttcat gaaagattat ctctggctct tcaggatgcc aacaaaatga
    28141 agattgcccg cgaagatttc ttgcgcatca gccgctccat ttgttctgag aacgacaagc
    28201 ccctctatac ggcggttcat ggatatcata ccctctctcc ccagcagctt gccgtgcttg
    28261 cccaactgtg ccattaccgc gaacgcgtcg ccatgcaggc tgaccttcca cctttcaaaa
    28321 tcttgagcag ttctgtcttg ctggatatcg ccaggtcaga tatttcctcg atcaccgatt
    28381 tatcttccat ttcaggtctc tccgaaaaac ttcttcatcg ccatgcccgg ggcttgctcg
    28441 atgccatcca acagggcagg tctgacccgc ccattcatct cgatcgccga cccaagccgt
    28501 ctgatgcata catcgcccgt ctggaacgtt tgaaaaaatg gcgccagcac ttggctcgcg
    28561 agaaagcggt ggaatccgat gtcatccttc ctcgcgagat catggaagag atcgccaagc
    28621 agcatccctc ttccccccag cagttagaag ccttgatgcg cgacgttccc gagcgctttc
    28681 aacaacacca gcatgccatt atgcatattc ttaaggagca ttcatgaaaa ttcatttcaa
    28741 tggcgctgcc cagacggtta ccggttctca gcacctcctg gagatcaacg ggaagaaatt
    28801 gctgctcgaa tgtggcatct accaggggaa acgcgcagaa ttctacagcc gcaactgccg
    28861 ttttcctttt gaactcgccg ggctggatgc ggtcattctc tcccatgctc atattgacca
    28921 cagcgggaac ctgcccaacc tggtcaagca gggatattcc gcacccatct acgccactcc
    28981 ccccactgcc gacctggcgg atatcatgct gcaagattcg ggtcatatct acgaagaaga
    29041 cgcccgctac ttaaatcaaa aaaagagccg ccgcgggctc cccccggtgc agccgctgta
    29101 caccaaagaa gacgctcaac gtgtaaatcc gctctttcag cgcgttggtt acggtcaggt
    29161 ctttgagccc atccccggcg tgcaggcgca cttgctggat gccgggcaca tgctgggctc
    29221 agccggggta tgtctggata ttaaggaaca tggtaagcaa acgcgcctgt ggttctccgg
    29281 cgatatcggt cgcccggggc ttgcgctcat tcgcgatccc cagcttcctg aaaatgcaga
    29341 gcatctcatc atggaatgta cttacggtga taagtcgcac agcgatcccg aagaggcttt
    29401 tatggaactg cgcgacgtca ttcagcgcac ggtggcgcgc aagggaaaga tcatcatccc
    29461 cgctttttct gtgggtcgca cccaggagat cgtgtacaac ctgaaccgca tgatccgcga
    29521 aggtgatctg ccatccatgc ctgtgttcgt agatagcccg cttgccatca atgccaccga
    29581 gatcttccgc aaacaccccg attacttcga tgatgaaacc cacgccttca tgcaaaccgg
    29641 acgccatccc gccttgacct tccctggtct ggagtacacc cgcagcgtgg aagaatccaa
    29701 agccatcaat gaaaaatccg ctccgctcat catcattgcc gcctccggca tggcagaagc
    29761 cgggcgtatc ttgcaccacc tgaaaaacaa cattgaggat gaacgcaaca ccatcgtgat
    29821 cgtctcctgg caagccccca acaccttggg gcgccgcctg gcagatcgcg aaccgctggt
    29881 gcgtattttc ggtgaagagt accggttgcg cgcggaggtg gctaccatcg gcggtttctc
    29941 ttcgcacgcc ggacagaact tcttgttgaa atacgcccaa gccacccacg ccagcctcaa
    30001 ggggctgtat cttgttcatg gcgacccggg cgctgccgct gttttccagc aggtgctggc
    30061 ggagcatcaa ttcaagtccg tcctctatcc gcccctgggc gcgagcgtga acaagttcag
    30121 atagatatga gagaggggag ctagacagga acggcaatcg gg
//
LOCUS       DAOSDS010000016        29675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_747_length_29675_cov_5.276671, whole genome shotgun sequence.
ACCESSION   DAOSDS010000016 DAOSDS010000000
VERSION     DAOSDS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 29675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29675
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_747_length_29675_cov_5.276671"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="PLL88_03465"
     CDS             <1..231
                     /locus_tag="PLL88_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00652.1"
                     /translation="WLVAKKIKCLGVDISGIEKRGIPDQPNHRLLFRNNIPLIEHLAN
                     LDQITQSRFFVVAVPIRMHGVDACPVSVIALV"
     gene            247..1206
                     /locus_tag="PLL88_03470"
     CDS             247..1206
                     /locus_tag="PLL88_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOJ00653.1"
                     /translation="MEISNDLYLAMFERMLKIRVFEEELYRLFMTEKMPGTMHQAIGQ
                     EASEVGVCMALIDGDAVVSNHRGHGHAIAKGVSIDELMAEMYGKKDGVSRGLGGSMHM
                     YDKKNDFFGTNGIVGAGAPIASGIGLALKLEHTGNIVVSFFGDGAINQGAVHEAMNLA
                     AVWKLPVIFVCENNRYAVSMPVEKSSVVKNLSERAKAYGIPGITVDGMDVVDVYNKTL
                     HFVNDARKNEGPAFIECKTYRYKGHSRFEPAKYRPAGELEEWKKVDPINKAKDSLINE
                     FKVNPAVVKQIEDAVLQEINAAVQKARRSETLAADEIANFVFE"
     gene            1219..2259
                     /locus_tag="PLL88_03475"
     CDS             1219..2259
                     /locus_tag="PLL88_03475"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012009660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOJ00654.1"
                     /translation="MEKITYAKAINQAMYEEMKRDSKVILIGEDVGAFGGAFQVTKGL
                     SDAFGEDRVFDTPISESAIIGCAVGVAMHGWRPIIEMQYSDFVTCGFDQIVNQAAKIH
                     FMSGGQFSVPLVLRMPTGAREHGAHHDQSTEAWFMHTPGLKVVFPSTAYDAKGLLKSA
                     VRENNPVVFFEHKVLYSSHSVGGQGLPDVVSNDFVQDIEKDIPDEEYLIPLGKADIKR
                     EGTDLTIIATGLMVHRTMQAALVLEHKNISAEVIDLRSLVPLDKSTILASVQKTNRVI
                     IVTEETRTASAASEISAMINEEAFDYLDAPVVRLGTPDTPLPFSPAMQDVLIPSVLNI
                     VEAGQELLRRCL"
     gene            2494..3675
                     /locus_tag="PLL88_03480"
     CDS             2494..3675
                     /locus_tag="PLL88_03480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOJ00655.1"
                     /translation="MPKEITMPNLGASSDESKLVKWIAKNGEKVTKRQIIFEAESDKA
                     VVEIEAAETGILKHVIHEGAIISSGQVVGLIYEDGESISEESSKPVKTENAPQSTNER
                     ALFESKPEKNRIIASPLAKKIAKANGIDLSLMRGSGTHNRIIKVDVENSRLDNRKTLS
                     GVKNSGTISGIRARIAEKMMQSHLTTVHSTLHSEVMADEIVELRGKLNLLLKDANIEI
                     SYDLILAIICAEALQRHPNILVQLENDQIVTKTEMNIGIAVDTPKGLMVPVLKRINQK
                     SLKSLAMEMDELIDRVKNGRGTVEDFSDGVFTISNLGLYRISSFTPIINYPESAILGV
                     GKITKQPIVRNSQIDMANVMTLSLTYDHRLIDGAPAARFLQLIGDYIESPALLFFQWE
                     R"
     gene            3751..4212
                     /gene="rpiB"
                     /locus_tag="PLL88_03485"
     CDS             3751..4212
                     /gene="rpiB"
                     /locus_tag="PLL88_03485"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOJ00656.1"
                     /translation="MSNNTQSILIGSDEIGLVLKNQLRDYLIEKNYAVIDLGVNTNEQ
                     VLYPQIAIALCEKLLREGYGRGVLVCGTGIGMAVTANKIPGIRAAVIHDPYSAERARA
                     SNDAQIATFGSLVIGVNAAKKNLDIWLGSEFAGGRSLPKVEMIKELDRRKE"
     gene            4752..5519
                     /locus_tag="PLL88_03490"
     CDS             4752..5519
                     /locus_tag="PLL88_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00657.1"
                     /translation="MATDNIETQYKLFHKYWQNGHDNESVKDYQIADNIRKKLIPAID
                     TIADEESRDFISSVYGEFADFLYFYFICRSQQGSNDSKILSEMEKMAEKAIELDPDSF
                     AGYYYLTVFHSCKLKTATAGKNPVVYKGQEMADTIVETAMNVLFKGITLGVTAAAAGI
                     SNEHFTTSILNLIEVYNRNLQREPASAVAYLDNTERMFRIADFCEGIKNDTWKEIYKA
                     IKTFDVNQLDYSDVIEERLEEAKEQAMEYYILADSKL"
     gene            5548..5784
                     /locus_tag="PLL88_03495"
     CDS             5548..5784
                     /locus_tag="PLL88_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00658.1"
                     /translation="MTNTLILVDRRDDPWRKANVTLSWRGGGYSKVWVDDSGRGQFGG
                     SGVVIEAQVAGEKIPVYQPVNGDTTIVVKSDRAH"
     gene            5874..6067
                     /locus_tag="PLL88_03500"
                     /pseudo
     CDS             5874..6067
                     /locus_tag="PLL88_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6154..7179)
                     /locus_tag="PLL88_03505"
     CDS             complement(6154..7179)
                     /locus_tag="PLL88_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ00659.1"
                     /translation="MPNQTSLAQEWLEKTNQAATPEEQIHCCNQALELDPEFALAYFY
                     RGNCYVGLHQYAEAIADYDRALRLDPKMDGALINRGICHAHLHQYEEAVGDHSQAIRI
                     NPENAEPYYFRGNCLFQLEKYEDAIGNFDQALRLDPEFAAAYNNRGLCYSNLDQDEKA
                     IEDYNQAARLAPENASVFYDRGTSYAVLRQHEKAIEDFTRAISLEPDFGDAYYNRGNC
                     YGHLKRFEKAIEDYSQSIRLDPDDAYAYNNRGFTYANLGQYEKAVEDCDRALSLDPEL
                     EDACYNKACACAHLGKMEDALSNLRQALRIAPHKYCDQAKDEPAFEAIRGDAVFRELL
                     EINCAEC"
     gene            7348..7541
                     /locus_tag="PLL88_03510"
                     /pseudo
     CDS             7348..7541
                     /locus_tag="PLL88_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7661..8023)
                     /locus_tag="PLL88_03515"
     CDS             complement(7661..8023)
                     /locus_tag="PLL88_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOJ00660.1"
                     /translation="MVKKSIETIKKELERKKEELISFLKPSTEHGEINPNDYDLSQQY
                     QLSEQQSSLNSYNQSILSQIEIALKKIEEGSYGVCERCRKPIDPERLAIIPYANFCMD
                     CLNRFPSKLIHSSTISEE"
     gene            complement(8051..9727)
                     /locus_tag="PLL88_03520"
     CDS             complement(8051..9727)
                     /locus_tag="PLL88_03520"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOJ00661.1"
                     /translation="MDKSDQKAHREVKMQQSVLKNGVSGNEKQILTLSTYLTKTDFTL
                     YVALPDNDPAKEKLFISIFGNTLSVNYEFFQHGMQSQSKMIALPDDVDVDAIRYEFEK
                     RILRLIIPRDDSIPHPQNSVLVLTFPPEKNIQKDKFDFPKIEIEKPVECIANPFMTLD
                     KPTNHVQEFFSTGIQNEVEQFHSTIPGFKASPLVSLPNLAAMLGVRSVWVKDESKRLN
                     LNAFKVLGGSYAIAKTVQHLLQPTNSSLNFDELTAKNTAPKLGKLIFAAATDGNHGKG
                     VAWAASQLGYQSIIYVHKLTSQARIRAIENSGAKVVVVDGTYDDAVRKVNWDAKENGW
                     LVISDTAWEGYEDIPRWVMQGYTTMLSETQRQFAEQGIERPSHVFVQAGVGALAAATI
                     GFYAARFKQNRPLNVVVEPGKAACLFHSMEIADGDPHSFAGNLDTIMAGLACGDPNPL
                     AWPIIREHADFFIKCPDYVAAKGMRVYGSPAQGDPAIISGESGAVTLGALMFLMQWDE
                     AKAFRKKLNLTEQSDVLLINSEGNTDPDHYRHVVWDGGEAVPAIYKSYWP"
     gene            complement(9751..10122)
                     /locus_tag="PLL88_03525"
     CDS             complement(9751..10122)
                     /locus_tag="PLL88_03525"
                     /inference="COORDINATES: protein motif:HMM:NF037143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ00662.1"
                     /translation="MASNSATATTSKPEATVEKFTTEPDLNNFPASIFSEVSFLEKKN
                     QFFVQIALNDTDFTNLKLNASENTLVLRIQGKQGNTFIRIITLPVHVNIEQGKADYSG
                     NILTICFPILSTSKEIEIQLK"
     gene            complement(10256..12466)
                     /locus_tag="PLL88_03530"
     CDS             complement(10256..12466)
                     /locus_tag="PLL88_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ00663.1"
                     /translation="MQKKSPSLTKGDVNVHIYADLFHHSATPAALFDTNKKCILANHS
                     FLVQLGYPAANHSHLNPDFLQIFEDKAQGTAFFEQLCERKIVRRHESHLITKNGHGFS
                     VLISGRVFNFNGDKFFQISFTDISRLKDLEETIRKDSAKIVSIMENSAAGLFYVSPEG
                     ILTNLNMKLLKMLDINENAYIGQPVKIWLGDLLAKTSEPEIIQKRIATALSDLDQYPK
                     IEFAITAGITFHYEMALFPVKGEMGESLGWGAIVQDITELKQQANWKLELLSILSHDI
                     RSPLATLKGHMSMLQENLTHWGDDIVKDFLNTISENIDKLSYQIDRNLALTRVEGGDL
                     GLRPQNIDLVQCVEQAIKFISDKKDKLVIDLIHDDNLAKTRADPARIEEVMINLLDNA
                     VRYNPPDKNLIIEIHSSGSWLIVSVRDFGKGVPADKQKTIFDKYVRVDEEGTGTGLGL
                     YISRKIVEAHGGQIRIQSPLPNMKHGTEFTFTLPATYSAPTKRTPLVKRQSLNRTANK
                     EFTILVIEDELDQLTLMKTILDEAGFQVETVTNGVSALDVVKISPPDLILLDWLLPGM
                     NGLAVCQGIRRYTNAPIIVVTSCTEQEELLTAFDAGADDYIIKPFDRQELFARIHALL
                     RRESAIQNPVKSNRFNAHGLVIDFDTHETWLDGENLLLPRAEYEILEYMAQHRNQLLT
                     YGQIIESVNSLMVEDSQNALYVHISRLRKKIEPDPKNPTFIITRWGIGYVFMPY"
     gene            12926..13879
                     /locus_tag="PLL88_03535"
     CDS             12926..13879
                     /locus_tag="PLL88_03535"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ00664.1"
                     /translation="MSSTVTPIPTFTPTITPTPKFTPTPLPTITPTPNPFAGYTIEAL
                     SARTYGGGVIQNEGVLTNGGAFTRYMVKYRSEGLELYGFLNVPDGEGLFPVVVMLHGY
                     IEPAEYSMLDYSTRYADALAKAGYIVVHPNLRGYGASDDAYDPVGIGDTIDTLNLLAL
                     IRTQAGASGLLEKADGERIGVWGHSMGGGIVLRVLEIDNDIDAAVLYASINADEKVNL
                     AYFDSDDGRGNPAIHTTDEVLRTLSASTYLDRITAPLSVHHGAADTVVPLEWSDQLCS
                     DLGALQKEVYCYFYENQPHTFQNSGDTLFIQRMVEFFDQQL"
     gene            13974..14447
                     /locus_tag="PLL88_03540"
     CDS             13974..14447
                     /locus_tag="PLL88_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00665.1"
                     /translation="MSEKDINEKDEKMMEEKNEKEQEKHEEKKEEQDLLSTLAWAAVL
                     IWAGLVFLGHNLGWWDAIGVSVRHSWFFRGVSELLAFNTFHMIALGAGVIVLIEVLLR
                     LVLPAFKSHVGGKIVLAAVLIGWGLSPFFNWYIIWPLILIAVGVSVMLGGLLKKK"
     gene            14515..14949
                     /locus_tag="PLL88_03545"
     CDS             14515..14949
                     /locus_tag="PLL88_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00666.1"
                     /translation="MDDLGLRDQDEIIINDRFATLIWAGMLIWMGGIILAGFQHWVAD
                     WRLPDLVWFPSLRNWLTSYRFDFRVIPLLFLGNAAILAFELLVRVAVKKLRYNLTMTF
                     LHFFIFLGLAAGSCGMFQPVMLIPGFILALGVSILLSIIFQR"
     gene            complement(14962..16113)
                     /locus_tag="PLL88_03550"
     CDS             complement(14962..16113)
                     /locus_tag="PLL88_03550"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOJ00667.1"
                     /translation="MPTRPDSNPMQQLQQMVEPWQRSIQDPFKAQQAVLEKFIAIYAH
                     TTYGRQHGTGNIGNIQDFRNAFPVQTYEEYKPLIQQVMAGEIDLLLNEQPVGWAITRG
                     TSKGEVKFIPMTPTDLKMRVSAGRAVVNFALRSGCFELFQGVNLNLNFPSVVGSIRVG
                     EKELEYGYSSGIYTRHVSALTPVRSVPSQDEIDALGGGKTQKDWDARFELAYTKCLPE
                     KVTLVGGVAPTALQFGRYLRRTHRHLPKDKWNVLVMTLGSVPGINTRLAQDLWGYYGQ
                     QAAIREIYGATEGMFGQQMDEKRAWVPNYDLFFFEVQTRQGVKMLHEMKPGESGSLIV
                     STPIFPRYQIGDRILAFQAPYFRCIGRNAWTTPLKYAWGEFNSLNLGRL"
     gene            complement(16226..16846)
                     /locus_tag="PLL88_03555"
     CDS             complement(16226..16846)
                     /locus_tag="PLL88_03555"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HOJ00668.1"
                     /translation="MENLQRTKHMQSILVTSTRTYVNNASIAADLAAMVAQSGKKTIL
                     LDADLNRPVIHRVFELPNRVGLSDVLQGQRTPAAIMHSMLEGNLHIVTSGNMPTMTKD
                     LIGTREMSNLLKQLKQEYEKIFIHGPAFFYNEATLLAAQVDGVVLMINPSYAKSEATQ
                     AIVERFQKTGATIIGIVMRDQPKYQSNQSTFINRLLNYDKHNRMER"
     gene            complement(16968..17099)
                     /locus_tag="PLL88_03560"
     CDS             complement(16968..17099)
                     /locus_tag="PLL88_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00669.1"
                     /translation="MKNLTSKVSLFVSHKGQFVLAILAIALFVLAAGAPNATLGIGR"
     gene            complement(17126..17371)
                     /locus_tag="PLL88_03565"
     CDS             complement(17126..17371)
                     /locus_tag="PLL88_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00670.1"
                     /translation="MLKKIEDYSYNRPAHDQEYSRLLSAAVINRNFCTMLLSDPSKAI
                     HNGYSGEKFNLSKEAEEKVTTIRATSLQEFAAKLAAL"
     gene            complement(17398..17520)
                     /locus_tag="PLL88_03570"
     CDS             complement(17398..17520)
                     /locus_tag="PLL88_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00671.1"
                     /translation="MKNTISLFVGRKGQLILAILTIALFVLAAGAPNATLGIGR"
     gene            complement(17551..17682)
                     /locus_tag="PLL88_03575"
     CDS             complement(17551..17682)
                     /locus_tag="PLL88_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00672.1"
                     /translation="MKSIINKINLFASHKGQLILAILTIALFVLAAGAPNATLGIGR"
     gene            complement(17713..17844)
                     /locus_tag="PLL88_03580"
     CDS             complement(17713..17844)
                     /locus_tag="PLL88_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00673.1"
                     /translation="MKNTINNIRLFASHKGQLVLAILTIALFVLAAGAPNATLGIGR"
     gene            complement(17870..18115)
                     /locus_tag="PLL88_03585"
     CDS             complement(17870..18115)
                     /locus_tag="PLL88_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00674.1"
                     /translation="MLKKIENYSYSKPAHDMECSRLLSAAVINRNFCTMLLSDPSKAI
                     HSGYSGEKFNLSKETEEKITTIRATSLQEFAAKLAAL"
     gene            complement(18150..18281)
                     /locus_tag="PLL88_03590"
     CDS             complement(18150..18281)
                     /locus_tag="PLL88_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00675.1"
                     /translation="MRNLTNKANLFVGDKGQLVLAILTIALFVLAAGAPNATLGIGR"
     gene            complement(18301..18552)
                     /locus_tag="PLL88_03595"
     CDS             complement(18301..18552)
                     /locus_tag="PLL88_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00676.1"
                     /translation="MLRTFNFTPILPTKTEKNTEMSRITCAAVINGPFCRMLLESPLR
                     AIEAGFHDEYFHVNAQDLAKIKAIHASSLVDFATQVANI"
     gene            complement(18536..19138)
                     /locus_tag="PLL88_03600"
     CDS             complement(18536..19138)
                     /locus_tag="PLL88_03600"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HOJ00677.1"
                     /translation="MVLLVAVVAGLIAALIRSWIRHEKFRSLKMRSVWLVFAAFLPQY
                     LATSLSFTRERIPDSWMPFILIGSLILLMVFVWLNRTEAGMWALGCGLFLNFLVIFLN
                     HGFMPLSPETAQKLTPPGVDVALTIGERVGFGKDILLPVEGTRLWFLSDIFVLPQWMN
                     YRVAFSIGDIFISVGAFLFLWSLGGSSQRKSMEAHNAQDI"
     gene            19491..20855
                     /locus_tag="PLL88_03605"
     CDS             19491..20855
                     /locus_tag="PLL88_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00678.1"
                     /translation="MQKKIPLWTKVVYGSGDFGYSMTNSIIAAYFLLFLTDVVGMSPA
                     VAGIAILIGRTWDYINDPLIGNISDRTHSRWGRRRPFLLFGAVPFAITFAMLWYKPAF
                     TDQTALAVYFALAYVLYDAAQTFVFMPYFALTPDLTDDYDERTSLTSYRMVYNIIGGL
                     VAFTLPLMLVKSFSPDHANRVFTMGLLFGLLSAVPLFFIFLKTREKNAVVKYEKPDIF
                     NSLKLALKNRPFVLGAVVYLATWTSMNVVQTVLLYYVKYVLEREAMSDILMGSIFITA
                     IIALPLWNWVSKKLDKRKAYALGISFWAIAQLLLMSLSASSSTVWVMALCVIAGVGVS
                     AAHVLPWAMIPDAIEYGELSTGERHEGVFYSLVTLMAKVASSIAIPLTAFVLEITHYV
                     PNSAVQPQTALNGIRFLMGPIPAILLIIGIVFALRYPLERKEFNAIVDKLNKKRTSQK
                     ELLQ"
     gene            20905..21789
                     /locus_tag="PLL88_03610"
     CDS             20905..21789
                     /locus_tag="PLL88_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOJ00679.1"
                     /translation="MAVHYKMASQSRNVRKERIWQVGVLSFFFLLINGCSLFLPTAEG
                     GTATLSIEEQVAGTLAAFQSTLALTLSGTAPLVEPGNDFSLEVTQEVFLATPTALADP
                     GDQELPTYHHIYNIWGHRQYFAIGCEAATATDWAAFFGVVVNEFNFQYQLPQSDNPDL
                     GFVGSVEGPWGQVPPYAYGVHAAPVAKVLREDYGLNAQGVKGFTLEELKAEIAANRPV
                     IAWVVGNCVGGVPYEYTDSQGNTTIVAAYEHVVIVTGYTEDTIRYMNNGKFYDIPTEY
                     FLNSWGILGNMVVYLETG"
     gene            complement(21800..22165)
                     /locus_tag="PLL88_03615"
     CDS             complement(21800..22165)
                     /locus_tag="PLL88_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOJ00680.1"
                     /translation="MKLIIRWAATSLALFAAAYLVPGIHVDSPEAWQVFAVMAIILGL
                     VNAVIRPILKVLSCPLIILTLGLFVIIINAVSFMAASNIAVNWFNVGFYVDDFWAALL
                     GSLIVSAVSIFVNMLFREE"
     gene            22270..23505
                     /locus_tag="PLL88_03620"
     CDS             22270..23505
                     /locus_tag="PLL88_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00681.1"
                     /translation="MHSDQKTRLKDLPRNIWAVSLTSFFMDISSEMVINVLPLFLSNV
                     LGVKTNIIGLIEGVAEATASLLKMISGWLSDKLKTRKWLAVLGYGISALAKPFFYFAG
                     SWGSIAAIRWADRVGKGVRTAPRDALVADSIEEKQRGLAFGFHRAADTFGAVLGLAVA
                     ALVVWLTQRSSGMLTVDTFRIIVLLSLIPAVLSVLALILGTKEVEIKGAHSLPKISLK
                     ALGKDFLIFLLIVSLFDIGNSSDAFLTLRAQERGVSVLGILIMLIVFNLVYTLISTPA
                     GILSDRVGRKKMIIGGWLVYAAVYLGFGLAQTTWHIYVLYVMYGIYYGLTYGTTKAMV
                     ADIVPVELRGTAYGTYNGIMGILDFPASLLAGILWQGVGKWGGFGPSAPFLFGAGTAF
                     AATIWMAVWVPATKKTDNG"
     gene            23548..23796
                     /locus_tag="PLL88_03625"
     CDS             23548..23796
                     /locus_tag="PLL88_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00682.1"
                     /translation="MQVITKKNKYFIGVALCVIAAFSFINGDYYSAVALLFVCVSALT
                     TIDKNDASVKNPQVWQGINLAGYVLAAAIWIYSFYMKK"
     gene            23810..24394
                     /locus_tag="PLL88_03630"
     CDS             23810..24394
                     /locus_tag="PLL88_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00683.1"
                     /translation="MQWKLGAYQVGNAWRSQQDVFKLLVTHFRRYHLVEIQDVYKLIY
                     QGVRGSSHILSSSPSDFEVNLRREFASVEANNSQPLWESIHPEGKIVRVNLAAYKYQS
                     QKIERLSALCLWTAEISTSSEKDVEHISTAFNTFYKLCKSRKIPKFAPLEVEKYHKWV
                     EENKYPVVHHSETYRTEYDPHYRLVHRDFLSVLF"
     gene            complement(24391..25608)
                     /locus_tag="PLL88_03635"
     CDS             complement(24391..25608)
                     /locus_tag="PLL88_03635"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HOJ00684.1"
                     /translation="MQIGIVGGGAAGMMAAILLQKEGLKPLLLDANASLGRKLSATGA
                     GRCNISNRSAKADAYFTDDPEGLHQCFRTVANARVLEILRSIGIPTTTTDDGWIYPLS
                     FSAANVVSIMENHLQHVSTFHNTLVTDIQKNEDHFALLTAERGKVHHVKTLLIACGSP
                     ANPQLGARGELYAALGRLGHHILPVRPALTPIETDPELFHKLQGVRLDAEVCLLHHHK
                     EITRTLGNVIITQWGINGPGVMDISHWIDPQKPQEYSLKINFIPKSSHAICDSLSDPA
                     LHKLSPAAVLQGYLPAKLADFFLKQTHMESVTTCGELTIEQKERLLHSMQEQPLSVRG
                     VRGFKYAQASSGGVALCDIDPLTMQSRVCSGLFFAGEIVNVLGSCGGFNLHWAFLSAI
                     LAAQAITQTANDL"
     gene            25717..26481
                     /locus_tag="PLL88_03640"
     CDS             25717..26481
                     /locus_tag="PLL88_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00685.1"
                     /translation="MEKHLYLSLLPEALIVSMLTPEEFGVYYAVGSLKKSRGQAIFFE
                     IDPDFRSDEFQIEEALKRCVPHEDGTPKRSLYVSVYRVLERIPLKTLRKLYLVTQDGR
                     TLGIEKSVQLPKEEEGMHLYREISPVNPLVVSTLNPKAFYELIVRNPTSLIKLPAVCF
                     AELKLGALADDPQNGDIGDLPYENIYHIRDCLLELRTKYVHSKMVDRTPSGGIPYRTI
                     KHGFFVGNEKELVHYPIPTQEELIKEHYSWWRSASF"
     gene            26569..28644
                     /locus_tag="PLL88_03645"
     CDS             26569..28644
                     /locus_tag="PLL88_03645"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOJ00686.1"
                     /translation="MSSQSWLLIGVGAGLVSVAVALYLYYWVKKQNAGSAKAQEVARW
                     IREGASSYLKKLYTSLGLVAIILGFLIALVFSFDLDNLGTSQITVDSAEGLRIAFTFI
                     FGAICSAVAGYLGMSVAVEANVRSATAAEKSLNQSFRVAFYAGSVMGLAMVGLAVVGM
                     STIFLITGDPELILGFSFGASTLALLAKAGGGIYTKTADIAADLVGKVEIGIPEDDPR
                     NPAVVADNVGDNVGDVAGMGADIFDSYVASAVAAILLGAALPNQSDTRYVVFPLILCA
                     LGIVASLIGIQFVRVGKDGKPGAALNRGTVITAMIYALMVSGLVLAGDYDKGALWATL
                     VGLVTGVVIGFTSDYFTSDAHKPVQETARVSGSGSAVTIITGFSYGLISIVPSVIGIV
                     VATLASYFLVEASGTLSGVYGIGISAVGMLAISGMIVSADAYGPIVDNARGIAEMSGM
                     DHEVIERADILDSAGNTAKAITKGFSISAAALTVLALFASYAEAIEASGYALTLSLRD
                     PIVIGGVLLGAMTPPLFSALTMLAVTHNAFDMIEEIRRQFREEPGILAGKVLPDYNTC
                     VSLAAQGALRSLVLPAFIAVGLPLLVGFLLGPNALGGFLGGAIFIGIVLALLMSNAGG
                     LWDNAKKYIESGVFGGPGSEAHKAAVVGDTVGDPFKDTAGPSINTLITVMSLVSTLFA
                     PVIAAFHLF"
     gene            28912..29568
                     /locus_tag="PLL88_03650"
     CDS             28912..29568
                     /locus_tag="PLL88_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ00687.1"
                     /translation="MTIPIFSHTDLSSLSERTKRHLESITKSTKVPKGEQFLWEGDEV
                     EKIYFIIRGYAKIYHFSDDGREQILAILDSGQWINTIPALRGEQGNHANGVAMTEMEL
                     GVMAARDFLDAIREFSDFALLILHDVADKLDHMTNLVAELSLYTTRQRLIHFLLEHCK
                     DKNTIHGWTQEEIASQVGTVRDVVSRLLGLFARQGYIRVERQRIIVLDEASLRNALNE
                     "
BASE COUNT         7446 a         6563 c         7706 g         7960 t
ORIGIN      
        1 tggcttgttg ctaaaaagat aaaatgtttg ggcgtcgata tatcaggcat agaaaagaga
       61 ggtatccctg accaacccaa tcaccgtttg ttatttcgta acaacattcc tttgatcgaa
      121 catttggcga atctggatca aataacgcaa tcgagatttt ttgttgtggc tgttccgatt
      181 cggatgcatg gtgttgatgc ctgtccggta tcggtgattg ctttggtata aggcaggagg
      241 ggccctatgg aaatttcgaa tgacctatat cttgctatgt tcgagcgtat gttaaaaata
      301 cgtgttttcg aagaagaact gtatcgattg tttatgacag aaaaaatgcc tggcaccatg
      361 catcaagcaa tcgggcaaga agcttcagaa gttggcgtct gtatggcatt aatagatggg
      421 gacgcggtcg tcagtaatca ccgtgggcat ggtcatgcga ttgcgaaagg tgtatcaata
      481 gacgaactga tggctgaaat gtatgggaaa aaagatgggg taagccgggg tctgggtggt
      541 tcaatgcata tgtacgataa aaaaaatgac ttttttggta ccaatggtat tgtaggtgca
      601 ggcgccccca ttgcttcggg gattggatta gccttgaagc ttgagcatac tgggaacatc
      661 gtggtttcat tttttggaga tggagccatt aaccagggtg cagttcacga agcgatgaat
      721 ttggctgcag tatggaaact ccccgtcatt tttgtttgtg agaataaccg atatgccgtc
      781 tcgatgcctg tagaaaaatc atcagtagtg aagaatctgt cggagagagc aaaggcatat
      841 ggaattccgg gaataacggt tgatggcatg gatgttgtcg atgtgtataa caagactctt
      901 catttcgtca atgatgccag aaaaaatgag ggtcctgcat tcatcgaatg caaaacatat
      961 cgctataaag ggcattcgcg atttgaaccc gctaaatatc gacctgcagg ggaattggaa
     1021 gaatggaaaa aagtcgatcc cattaataaa gccaaagatt ctctgataaa tgaattcaaa
     1081 gtgaatcctg cggttgtcaa gcagattgaa gacgcggttt tgcaagaaat caatgctgct
     1141 gttcaaaaag cacggagatc tgagaccctt gcagcggatg aaatcgccaa ttttgttttt
     1201 gaatagaagg taactagtat ggaaaaaatt acttatgcaa aagccatcaa tcaagcaatg
     1261 tacgaagaga tgaagcgcga ttcaaaagta attttgattg gagaagatgt tggggctttt
     1321 ggcggtgcat ttcaggttac aaagggtcta tcggatgcct tcggtgaaga tagagttttt
     1381 gataccccca tttcggaatc tgcaattatt ggatgcgcag ttggtgtagc tatgcacggc
     1441 tggcgaccaa tcatcgaaat gcaatattcc gattttgtta cttgtggctt tgaccaaatt
     1501 gttaaccaag ccgcaaaaat tcatttcatg tctggcggtc aattttccgt tcccttggtg
     1561 ttacgtatgc caactggggc acgggaacat ggagcacacc atgaccaatc tactgaagca
     1621 tggtttatgc acacacccgg attgaaggtt gtatttccat caaccgcata tgatgcaaaa
     1681 ggtttgctga aatccgcagt gcgagagaat aatccagttg tattctttga gcataaagtt
     1741 ctttacagtt ctcactcagt cggtggtcag gggttacctg atgtagtttc taacgatttt
     1801 gtgcaagaca tagaaaaaga tattcctgac gaagaatatc taatccctct tggaaaagca
     1861 gacatcaaac gtgaagggac cgacctgacg attattgcaa ccggcttgat ggttcatcga
     1921 acgatgcagg cagccctggt ccttgagcat aaaaatatca gcgcagaggt catcgatctt
     1981 cgttcgctgg ttccgctgga taaaagcacc atacttgcat cagttcaaaa aacaaaccgg
     2041 gtaatcattg tgacggagga aacccggacg gcatctgcgg catccgaaat atcagccatg
     2101 atcaacgaag aagcgtttga ttatctggat gccccagtcg ttcgtttagg aaccccggat
     2161 acaccgttgc ccttttctcc cgcaatgcaa gatgtgttga tccccagcgt tttaaatatc
     2221 gttgaggctg gacaggaatt gttaagaaga tgtttgtgat ccggaatcat gaaaaaacag
     2281 tgatggtttg cctgatcaag ttggaaggga aaattcgcga ctgagaagag aaaacctgtt
     2341 acaaagggag actcctggtg aaggttaagg cttgtttacg tatcgttggt ggtatggtga
     2401 tcggttttcc tgaagaactc aaccctatat ggttttcgaa caagaattta cttggcagca
     2461 ggctcaaaca gcagaaggag tgatggaaga aatatgccaa aagaaataac catgccgaat
     2521 ttgggtgcat catcggatga atcgaaactg gttaaatgga ttgcgaaaaa cggagaaaaa
     2581 gtaaccaaac ggcaaatcat ttttgaggct gaatctgata aagctgttgt tgaaatagaa
     2641 gctgcggaaa ccggaattct taaacatgta attcacgagg gcgcaataat ttcttcgggt
     2701 caagttgtgg gtttgatcta tgaagatggg gaaagcattt ctgaagaatc atcgaaaccg
     2761 gtcaaaacag aaaacgctcc ccaaagtacc aacgagagag cgctattcga atcgaagcca
     2821 gaaaagaatc gaattattgc atcgccatta gccaaaaaaa tagcgaaagc aaatggcatt
     2881 gatttgtccc tgatgagggg cagtggtact cacaaccgca tcattaaagt tgatgtagaa
     2941 aatagtaggc tcgataaccg aaaaacactc tctggagtca aaaatagcgg aacaatatca
     3001 ggtatcagag cgagaattgc agaaaagatg atgcaaagcc atctaacaac tgttcactcc
     3061 acccttcatt cggaggtaat ggctgatgaa atagtggagt taaggggaaa attaaatctc
     3121 ttgttgaaag atgccaatat cgagatctct tacgacctca ttttagccat aatttgcgct
     3181 gaggctttac aacgtcatcc aaatattctt gtacagcttg aaaacgacca aattgttacc
     3241 aaaactgaaa tgaatattgg aatcgcagtg gataccccca aagggcttat ggttcctgta
     3301 ttaaagagga tcaatcaaaa atcgctcaag tcccttgcaa tggagatgga tgaattgatc
     3361 gatcgggtga aaaacggcag aggtaccgtt gaagattttt cggacggcgt tttcacgata
     3421 agcaatcttg ggttatacag aatctcatcc ttcacaccca tcataaatta tcctgaaagt
     3481 gccattctgg gtgttggaaa gattacaaaa cagccgatcg tgcgaaatag ccaaatcgat
     3541 atggcgaatg tgatgacgct tagcctgaca tacgaccatc ggctgattga tggagcgccg
     3601 gcggcaagat ttttgcagtt aataggtgat tatattgagt cacccgcttt gttgttcttt
     3661 caatgggaaa ggtagcagat tttatcaaat tatttgtgtt aaaaacctga attagttgag
     3721 atggtaaatt gctagagaga aggaaaataa atgtctaaca acacgcaatc tattttgata
     3781 ggatccgatg aaatcggtct ggttctcaaa aaccagttga gagattattt gattgaaaaa
     3841 aactatgctg tgatcgattt gggcgtgaat acaaatgagc aagttttgta cccccaaata
     3901 gcgatcgcgc tatgcgaaaa gttattgaga gagggttatg gcagaggagt tcttgtctgc
     3961 gggacaggaa taggaatggc agtcactgcc aataaaatcc cgggtatcag agcggcggtt
     4021 atccatgacc catattcggc agagagggca agggcaagca atgacgcgca aattgccact
     4081 ttcggcagtt tggttattgg ggttaacgca gccaagaaga accttgatat ttggttagga
     4141 agtgaatttg caggaggaag gtcattgccg aaagtagaga tgattaaaga attggataga
     4201 agaaaagaat aaactgcgcg ttggtggaac gatattaatt tcttaagagc tatattgtcg
     4261 acgaattctt tgacgggtcg attgtggcag atagtaaggg atggctaagt atgaggggac
     4321 atggagaccc cttcaagggg tagttggaaa aagagaatcc ctttagggat gtgcggagga
     4381 ggaaagacaa agtggcttga acgagagtga aagccagcat cttgagaggg tgaccagtag
     4441 taaaggtttc tagccttaga gccccgtttt acgggtggtt tggaatggag ccaggtattt
     4501 gacgatggca cctttgcatt cgttgatagc atgctggcag ctcagcggca gagtgtgcgg
     4561 ttcataaccg cttggtcgca ggttcgaatc ttgccgggcc cataaaaaga tgcaaaaaga
     4621 tatttctttt tgtatctttt ttgaaaacgg aactcattaa aaaaattctt atataataaa
     4681 aaagtatata ggcagtatgg aaataatagt aaattctaat ttacaaaaat cgatatcaag
     4741 tggagattaa tatggctaca gataatattg agacccagta taaattattt cataagtatt
     4801 ggcaaaatgg ccatgataat gaaagtgtca aagattatca aatcgctgac aatattcgga
     4861 agaagctgat ccctgcaata gacacgattg ctgatgagga aagccgagat tttatttcga
     4921 gcgtttatgg ggaatttgct gatttcttgt atttttattt catttgccga agccagcagg
     4981 gcagtaatga ttcaaagatt ttgtctgaaa tggaaaaaat ggcagagaaa gcgatcgaat
     5041 tggatcctga cagttttgcg ggatactatt atctgacagt gttccattca tgcaaattaa
     5101 aaactgcgac tgcgggaaaa aacccggttg tttataaagg acaggagatg gcggatacta
     5161 tcgtggaaac agccatgaac gtccttttca aaggcattac tctgggggta accgctgctg
     5221 cggcagggat atccaatgag catttcacga ccagcatcct caatctgatt gaggtatata
     5281 accgtaatct gcaaagggag cccgccagcg cggtggcgta tttagataac acggaaagaa
     5341 tgttcaggat agcagatttt tgtgaaggaa tcaaaaatga tacgtggaaa gaaatatata
     5401 aagcaatcaa aactttcgat gtcaaccaac ttgattactc cgatgtaatt gaggaaagat
     5461 tggaagaagc gaaagaacaa gccatggaat actacattct ggcagattca aaattataaa
     5521 catcaaaaaa gataaacgag gacaaaaatg acaaatactt taatcctggt agatagacga
     5581 gatgatccgt ggagaaaggc aaatgtgacg ctctcatgga gaggcggtgg ttacagtaaa
     5641 gtatgggtgg atgattcagg cagaggacag ttcggcgggt cgggtgtggt gatcgaggcg
     5701 caagtggcag gagaaaaaat accggtatat caaccggtca atggggatac caccattgta
     5761 gtaaaatcag atcgagccca ttaagaattt tccatattga taacaaatat gcttgaaatc
     5821 ggtatcaaaa agacttatag atcttgtata gagaacccat tattcgcatt atttataggc
     5881 ggaagataat ctacaacaag ttaaagaggg atatagtaga ggtaatggta ataaaggaat
     5941 tatgcaagtg gaaaaaagta gagttcctag agggaaagcg atagtagatc acaatcacat
     6001 acacccgctg gtaagcatag cgtcaaagat cagtgtggcg agtttcatgg ggtatctgaa
     6061 ggggaagtgc gtgcggttca tcaccgcctg gtcgcaggtt cggactctgc cgggcccacc
     6121 aattttttcg gggtaattca gatagccgat gtgtcaacac tcagcgcaat tgatttccaa
     6181 cagctctcga aaaaccgcat ccccccgtat cgcttcaaaa gcgggttcat cctttgcctg
     6241 atcgcagtat ttatgcggag cgatccggag cgcctggcga aggttgctca aagcgtcttc
     6301 cattttcccc aggtgagcgc aggcacaggc tttgttgtag caggcatcct ccaattcggg
     6361 gtcgagactg agcgcccggt cacaatcttc aaccgccttt tcatactgcc ccaggttggc
     6421 gtaggtgaat ccgcggttgt tgtaggcata ggcatcatca gggtccaggc ggatggactg
     6481 gctgtaatcc tcgatcgctt tttcaaaccg cttcaggtga ccgtagcagt tgccgcggtt
     6541 gtagtaggca tccccaaaat caggttcaag gctgatcgcc cgtgtaaaat cttcgatcgc
     6601 tttttcatgt tgccgcagga ctgcataact ggtcccgcgg tcgtaaaaga cagaagcgtt
     6661 ttcaggcgcg aggcgggcag cctgattgta atcttctatt gctttttcat cctggtccag
     6721 attggaatag cagagtccgc ggttgttgta agccgccgca aattccgggt caagccgcag
     6781 cgcctgatcg aaattcccga tcgcatcttc gtatttttct aattgaaaaa gacaattgcc
     6841 gcggaagtaa tagggctcgg cattttcagg attgatccgg attgcctggc tgtgatcacc
     6901 aactgcttcc tcatactggt gcagatgtgc atggcagatc ccgcggttga tcaaagctcc
     6961 atccattttg ggatcgaggc ggagtgcccg gtcataatca gcgatcgctt ccgcatattg
     7021 gtgcagaccg acatagcagt tgccacgata aaaataagca agcgcaaatt cgggatccaa
     7081 ttcgagagcc tgattgcagc agtgaatttg ctcttcgggc gtggcagctt ggttggtttt
     7141 ttcaagccat tcttgagcta atgatgtttg attcggcatg taattgagtt aatcctcgaa
     7201 aagatgattg gaaaattcaa ttatatatct ccacaatgat agaggtaata ctaactagtt
     7261 gttgatttaa attctaagta aataataatt gtaattaaaa gtcactagta tcgttatctg
     7321 ttaggttttt caaccaaatt ccaatggatg tgcaaatatc atatagtgtt tacaccgaag
     7381 tacggagggg aaatattgag gcagttatgc gggtacaaag gagtaaaggt ccaggaaggg
     7441 cactcaatgg tagatcatgt gcgctgttgg taagcatagc gtcaaagatc agtgtagcga
     7501 gtttcatggg gtatctgaag aggaagtgcg cgcggctcat caccgcctgg tcgcggactc
     7561 gaaccctgcc ccgggttcat attcgttgct ccctttttct acagcgcaac ttttgcaacc
     7621 agcaaactaa aaaattatcg caaatcgcgc actcttttca tcactcctcg cttatggtag
     7681 aagaatggat caacttggat ggaaaacgat tcagacaatc catacaaaaa ttggcatagg
     7741 ggatgattgc caagcgctcc gggtcgatcg gttttcggca tcgttcgcac actccatagc
     7801 tcccttcttc aattttcttt aacgcgattt ctatttgaga aaggatgctc tgattgtaag
     7861 agttcagaga actttgctgt tctgatagtt gatactgctg ggataaatca tagtcattgg
     7921 gatttatttc tccatgttca gtcgaaggct tcagaaatga aatgagttct tctttttttc
     7981 tttcaagttc ttttttgatc gtttcaatac ttttcttaac cattcctcat ttcctttcca
     8041 atggattttt ctacggccaa taacttttat aaattgcagg tactgcttcc cccccatccc
     8101 atacgacatg gcggtagtga tctggatcgg tgttcccttc ggaattaatg agaagcacat
     8161 ccgactgttc ggtcaggttc aatttctttc ggaaggcttt cgcttcatcc cactgcatca
     8221 ggaacatgag cgcccccagg gtcaccgctc ctgattctcc tgatatgatg gcagggtcac
     8281 cctgggcggg tgatccatat acgcgcattc ctttcgcggc gacataatcg ggacatttga
     8341 tgaaaaaatc ggcgtgttcc cgtatgatcg gccatgccag gggattggga tcaccgcatg
     8401 ccagaccagc catgatcgtg tccaaatttc ctgcaaagga atggggatcc ccatctgcga
     8461 tctccatgga atggaaaagg caggctgcct tgccaggctc aaccacaaca ttcaacggac
     8521 ggttttgttt gaaacgagcg gcatagaatc cgatagtggc tgctgccaat gccccaaccc
     8581 ctgcctgtac aaaaacatga ctgggtctct cgatcccctg ttcagcgaat tgtcgttgtg
     8641 tttcagaaag catggttgta tacccctgca ttacccacct gggaatatcc tcgtagcctt
     8701 cccaggcggt atcagaaatt accagccacc cgttttcctt ggcatcccag ttcacttttc
     8761 gaaccgcgtc atcataggtg ccgtctacca ccacgacttt tgctccactg ttctcgatag
     8821 ctcgaatgcg ggcttgcgag gttaatttgt ggacgtagat gatggattga taacccaatt
     8881 gcgaagccgc ccacgctact cccttcccat gatttccatc cgttgcagcc gcaaaaatta
     8941 attttcccaa cttcggagct gtatttttgg cagtcaattc atcgaaattt aaagaggaat
     9001 tcgtgggttg taaaaggtgt tgtacggtct tcgcgatcgc ataggaccct cctaacactt
     9061 taaaagcgtt caagttcaag cgttttgatt catctttcac ccatacgctt ctgacgccca
     9121 acatagctgc cagattggga aggcttacca aaggacttgc tttgaagccg gggatggttg
     9181 aatgaaattg ttccacttca ttctgaattc ctgtggaaaa gaattcctga acatgattcg
     9241 tcggcttgtc gagtgtcatg aatggatttg cgatgcattc gaccggtttt tctatctcta
     9301 ttttagggaa gtcgaattta tctttttgga tgtttttttc gggcggaaag gtcagcacca
     9361 gcacggagtt ttgtggatgg ggtattgaat cgtctctggg aatgatcaat cgcaagatgc
     9421 gtttttcaaa ttcataacgg atagcatcca catcaacatc atcgggtaat gcaatcatct
     9481 tggattggct ttgcataccg tgctgaaaga actcatagtt gacgctcaag gtattgccga
     9541 atatcgaaat gaacaacttt tctttcgctg ggtcattgtc agggagagcc acgtaaaggg
     9601 taaaatcagt ttttgttaag taggtgctca aggtcagaat ttgtttctca tttccagaaa
     9661 cgccattttt caatacagat tgctgcattt tcacctcgcg atgggctttt tgatccgatt
     9721 tgtccatttt ttcatgaaca caaagcggtt ttatttcaac tgaatttcga tctctttgct
     9781 ggttgagagg atggggaaac agatcgtaag aatgtttccg gagtaatcgg cttttccctg
     9841 ctcgatattt acatggaccg gcaaggttat gatacgtatg aaggtgttgc cctgcttccc
     9901 ctgaattcgc aaaaccaaag tattttccga agcgttcaat ttcaaattcg tgaagtctgt
     9961 atcatttaat gcgatctgaa caaagaactg gttcttcttt tcgagaaagc tcacttcaga
    10021 aaaaatggat gctggaaaat tgtttaaatc aggttcggtt gtaaacttct caaccgtcgc
    10081 ttcgggcttt gaagtcgtcg cagttgcgga atttgatgcc atatttgctc ctagaatgta
    10141 aggctgtttg aggtcactca ttcgattcta ggatatagat ctttcaaagt ccttgctata
    10201 tcgaggagac atcttgcaaa attcttatgg atttttgaat caattgggag atatgtcaat
    10261 aaggcataaa cacatatccg attccccaac gagtgataat gaacgttggg ttcttcggat
    10321 caggctctat ttttttcctc aatcggctta tatggacata aagcgcattt tgactgtctt
    10381 ccaccattaa tgaattgacc gattcgatga tttgcccgta agtcagtaat tggttgcgat
    10441 gttgagccat atattccaga atctcgtatt cggctcttgg taacagcaaa ttttccccat
    10501 ccaaccacgt ttcatgggtg tcaaagtcga tcaccaagcc atgcgcattg aatcggttgg
    10561 actttacagg attctgaatc gcactctctc gtcgcagcag ggcgtgtatc ctggcaaata
    10621 attcttgccg gtcaaaaggt ttgatgatgt agtcatcggc gccggcatcg aaagcagtca
    10681 acagctcttc ttgctccgtg caggatgtga ccacgatgat gggagcgttt gtataacgcc
    10741 ggattccctg gcaaactgcg agcccgttca ttccgggcaa cagccagtcg agcaggatta
    10801 gatcgggagg agaaatttta actacgtcaa gggcagaaac gccattggtc accgtttcga
    10861 cttggaaccc ggcttcgtcc aggatcgttt tcatgagcgt taattggtcg agttcatctt
    10921 caatgacaag gatggtgaat tccttgttgg ctgtgcggtt gagcgactgc cgtttgacaa
    10981 ggggcgttct cttggtgggt gcggaatagg tggctggaag agtgaatgtg aactcggttc
    11041 catgcttcat attgggcaaa ggactctgaa tgcgtatctg accaccgtgg gcttcaacga
    11101 tcttgcggga gatgtacagt cccaatccgg ttccagttcc ctcttcgtcc acccgtacgt
    11161 atttgtcaaa gatcgttttc tgcttatcgg cagggacccc tttgccgaaa tcacggaccg
    11221 aaacgatgag ccaggaaccg ctagagtgaa tctcaataat gaggttctta tcaggcgggt
    11281 tgtaacgaac ggcattgtca agcagattga tcatcacttc ttcgatacgc gcaggatcgg
    11341 ctcgcgtttt tgcaaggttg tcatcgtgaa tgaggtctat caccaattta tcttttttgt
    11401 cggaaataaa tttgatggct tgttccacac actgaaccag gtcgatgttt tgcgggcgga
    11461 gccccaggtc accgccttcc actctggtaa gagccaggtt ccgatcaatc tgataggaaa
    11521 gcttgtcgat attttcgctg atggtattga ggaaatcctt aacaatgtca tctccccagt
    11581 gggtgagatt ttcctggagc atcgacatgt gacccttcag ggtggcaagt ggcgaacgaa
    11641 tatcatggga gagaatggat agcagttcca acttccaatt ggcttgctgt ttcaattcgg
    11701 tgatatcttg aacaatcgct ccccaaccca aggattctcc catttcgccc ttgacaggaa
    11761 ataatgccat ctcgtaatgg aatgtaatac cggctgtaat tgcgaattct attttggggt
    11821 attggtctaa atcggagaga gcggtcgcga tccgtttctg gatgatttcg ggctccgaag
    11881 tttttgcgag taagtccccc aaccatattt tgaccggctg tccaatgtac gcgttttcgt
    11941 tgatatccag catcttcaat agcttcatat tcagattggt caggatgcct tcagggctta
    12001 catagaataa cccggcagcg ctgttttcca ttatggaaac aatcttggcg ctgtctttgc
    12061 ggattgtctc ttcgagatct ttaagacgcg aaatatcggt aaaggagatt tgaaaaaatt
    12121 tatctccgtt gaaattgaac acacgccccg agatcaagac ggaaaaacca tgcccatttt
    12181 ttgtgatcaa gtgactttcg tgcctgcgga caatctttct ctcgcagagc tgctcgaaga
    12241 aagcggttcc ttgcgcttta tcttcgaaga tttgcagaaa atccgggttt agatgcgagt
    12301 ggttggcagc aggatatccc agttgaacca agaacgagtg gttcgctaga atacattttt
    12361 tattcgtgtc gaacaatgcc gccggggttg cagaatgatg gaagagatct gcgtatatgt
    12421 gaacattgac atctccctta gtgagggaag gggatttctt ttgcatttct atgcttaaac
    12481 cggtccttaa tgacaatcat acttatcagt attctatcag tcatcctgct ttcctccaaa
    12541 ccatttcttt gtgatgggag gttcatctga ccggaatcga gcgctgagaa acaagggatt
    12601 ggttgactaa taatcatgct gtacattgtg aaattttctg gaaattaatc ggtaagaatc
    12661 cgcacgcatc ttcgaagtcg gagatcgggg ttttgtcaag tttttaactg aacaggagcg
    12721 agattggcag cattttctta atcgaggaag ctttttctga attgaaaatc atagtattat
    12781 cactggcatg tttatcacaa ggaaatcagg agcgatccat aagcccacct tgcggattgt
    12841 tcttttcata accacgatcc ttttgtctgt tctctacact acctcctgca gccaggagcc
    12901 cctccctgcg ccgcaggtgc aacgcatgag ctctaccgtc acacccatcc caaccttcac
    12961 acccaccatt acccccaccc caaaattcac ccccacgcct ctgcccacca tcacccctac
    13021 gcctaacccc tttgccggct acaccatcga agcgctcagc gcgcgcacat atgggggtgg
    13081 cgtcattcag aatgaaggcg tgctcaccaa tgggggcgct tttaccagat acatggtcaa
    13141 ataccgcagt gagggattgg agctgtatgg ctttttgaat gttccagatg gcgagggact
    13201 ctttccggtg gtggtcatgc tgcacggcta tattgagccg gcggagtaca gcatgctgga
    13261 ttattccacg cgctacgccg atgcgctggc taaagccggc tacattgtgg tgcatcccaa
    13321 cctgcgcgga tacggggcat cggatgatgc ctatgacccg gtgggcatcg gcgataccat
    13381 cgataccctg aacctgctgg cacttatccg cacccaggcg ggcgcatccg gtctgctcga
    13441 aaaagcggat ggggagcgca ttggcgtgtg ggggcacagc atgggaggcg gcattgtgtt
    13501 gcgcgtgctg gaaatcgata acgatattga cgccgcggtt ttgtatgctt ccatcaatgc
    13561 ggatgaaaaa gtcaatctgg catatttcga tagcgatgat gggcgcggca atccggctat
    13621 ccacaccacc gatgaagtgc tgcgcaccct ttctgccagc acctacctcg accgcattac
    13681 cgctcccctg agcgtccacc acggcgcagc agatacggtg gtgccgctgg aatggtccga
    13741 tcaattgtgt tctgacctgg gagccttgca aaaagaagtg tattgttatt tttacgaaaa
    13801 ccagccgcac acttttcaga acagcggcga tacgcttttc atccagcgca tggtcgaatt
    13861 cttcgaccag caattgtagt gaacgagtgc gggcttccgc agcgggtgca actcatttcc
    13921 atttcttccg tattaagtaa tgtgagcaat taagaattag gtggaggaat gctatgtctg
    13981 aaaaagatat caatgaaaaa gatgaaaaga tgatggaaga aaagaatgaa aaagagcagg
    14041 agaagcacga ggaaaagaaa gaggagcagg atctgctgag cactctggcg tgggcggcgg
    14101 tgttgatatg ggctgggctg gtttttcttg ggcataacct gggctggtgg gatgccattg
    14161 gggtgagcgt gcgccattcc tggttcttcc gcggtgtgag tgaattgctg gcgttcaaca
    14221 ctttccacat gatcgcgtta ggtgcgggcg tcatcgtgct gatcgaagtg ctgctgcgcc
    14281 ttgttctgcc ggctttcaaa tcgcacgtgg gcggcaagat cgttctggcg gctgttctga
    14341 tcggctgggg gctttctcct ttcttcaact ggtatatcat ctggccactc attttgatcg
    14401 cagttggggt gagcgtgatg ctgggcggac tgctaaagaa aaaatagata aattggtctt
    14461 aaaaacggtg gcttggtaca acacaagcca ctttttgttt tataattgag cgccatggac
    14521 gatcttggat tgcgcgatca ggatgagatc atcatcaacg accggtttgc aacgctgatt
    14581 tgggcgggca tgttgatctg gatgggggga attattctgg caggttttca acattgggta
    14641 gccgattggc ggctgcccga tctggtatgg ttcccttctc tgcgcaattg gttgacaagt
    14701 taccgctttg actttcgcgt catcccgctg ctctttttgg ggaatgctgc catccttgcc
    14761 tttgagctgc tggtgcgcgt ggcggttaaa aagctgcgct acaacctgac catgaccttt
    14821 ctgcatttct ttatttttct gggtctggcg gcaggcagtt gcggcatgtt ccaaccggtc
    14881 atgctcatac ccggattcat cctggcgttg ggggtttcta tcttgctcag cataattttt
    14941 caacgctgac gtggaaagcc tttataacct tcccaggttc aaagagttga attcgcccca
    15001 ggcatatttc aagggggtgg tccaggcgtt tctgccgata cagcggaaat agggcgcctg
    15061 aaatgccagg atgcggtcgc cgatctgata gcgcggaaag atcggtgtgg atacgatcag
    15121 gctgccgctt tcaccgggtt tcatctcgtg cagcattttg accccctggc gggtttgaac
    15181 ttcaaagaag aacaggtcat aattgggcac ccaggcgcgc ttttcgtcca tttgctgacc
    15241 gaacatgcct tcggtcgcgc cgtagatctc gcggatggca gcctgctgtc cgtaataccc
    15301 ccacaggtct tgcgccaggc gggtgttgat gccgggcaca gatcccagcg tcataaccag
    15361 cacgttccat ttgtctttgg gcagatggcg atgcgtccgg cgcaggtatc tgccaaactg
    15421 taacgcggtg ggcgccaccc cacccaccaa ggtcaccttt tctggcaggc acttggtgta
    15481 cgccagctcg aagcgcgcgt cccaatcttt ttgagttttt ccaccaccca gcgcatcgat
    15541 ctcatcctgc gagggcacgg agcgcaccgg ggtgagggcg ctgacgtggc gggtatagat
    15601 gccggaactg tacccgtatt ccagttcctt ttcacccacc cggatgctcc ccacgacgct
    15661 ggggaagttc agattgaggt tcacaccctg aaagagctca aaacatccgg agcgcagggc
    15721 aaaattgacc actgccctgc cggcagaaac gcgcatcttc aggtcggtgg gggtcatggg
    15781 gatgaacttg acttcccctt tgctggtgcc gcgtgtgata gcccatccaa ccggctgctc
    15841 gttcaacagg agatcgatct ctccagccat cacctgctgg atgagcggtt tatattcttc
    15901 gtaggtttgt acagggaagg cgtttcggaa atcctgaata ttcccgatat tgcccgttcc
    15961 gtgctgcctg ccgtaggtgg tgtgtgcgta gatcgcgatg aatttttcca acactgcctg
    16021 ctgcgctttg aagggatcct gaatggagcg ctgccagggt tcgaccattt gttgcaattg
    16081 ctgcatggga ttcgagtctg ggcgggtggg catggggaaa ttcctttttg agtacattgt
    16141 agatgaattc tatttaaaaa gcagggctgc tttttgatga aagcagccag tgggtagaga
    16201 attaccggta accagatagg atggtctatc gttccatgcg gttgtgttta tcatagttca
    16261 gcaagcggtt gatgaaagtg gattggttgg actgatactt gggctggtcg cgcatcacga
    16321 taccgatgat ggtagcgccg gttttttgga agcgctcgac gatagcctgg gtggcttcag
    16381 acttggcata actggggttg atcatcaaca ccaccccgtc cacttgagca gcaagcagcg
    16441 tggcttcgtt gtagaagaat gccggaccgt gaatgaatat cttttcatac tcctgcttca
    16501 actgcttgag cagattgctc atttccctgg tgccgatcag gtctttggtc atggttggca
    16561 tattgccgct ggtgacgata tgtaaattgc cctcaagcat ggaatgcatg atggcagcgg
    16621 gcgtgcgttg cccctgcagt acgtcgctca aaccaacccg gttgggcagt tcaaaaacgc
    16681 gatgaatgac cgggcggttc aggtctgcat ccagtaatat cgtcttcttg cccgactggg
    16741 ctaccatggc agccagatca gccgcgatgg aggcgttgtt cacatatgtc cttgtgctgg
    16801 tgacaaggat ggactgcata tgtttggttc gctgtaaatt ttccatgtaa ttttctatcc
    16861 actaaacatt aaacgaacgc aaaacggtaa tgaaagagca agtttcatac cagaaggaag
    16921 ggcatggctt gccctctggt aaaaatacag ttcagcaaaa aaccagttta gcgaccgatg
    16981 ccgagagtgg cgttgggggc gccggcggct aaaacgaaca gagcgatggc gagaatggca
    17041 aggacgaact gtcctttgtg gctaacgaac aggctgacct tactggttag attcttcatg
    17101 atatttctcc ttcaaacatt catattcaga gtgcagccaa cttggcggca aactcctgca
    17161 gggaagtggc gcggatggtg gtgaccttct cctcggcttc tttgctgaga ttgaatttct
    17221 caccactgta accgttgtgg atggctttgg agggatcgga aagcagcatg gtacagaaat
    17281 tgcgattgat gacggcggca gaaagcaacc ggctgtattc ctgatcgtgg gctggtctgt
    17341 tgtaactgta atcttcgatt ttcttaagca tgatcgacat tctcctgaca aagtcggtta
    17401 gcgaccaatg ccgagggtgg cattgggggc gccggcagcc aaaacgaaca aagcgatagt
    17461 gaggatggct aagataagct gtccttttct tccgacgaat agactgatgg tgtttttcat
    17521 gatttttctc cttatcgatt ctgtaataga ttaacgacca ataccgaggg tggcattggg
    17581 ggcgccagcg gctaaaacga acaaagcgat ggtgaggatg gcgagaatga gctgtccctt
    17641 gtggctggcg aatagattga ttttgttaat gatgcttttc atgatttttc tcctgattta
    17701 tttgtgaacg aattagcgac cgatgccaag ggtagcgttg ggggcaccgg cagccaggac
    17761 gaacaaagcg atggtgagga tagcgaggac gagctgtccc ttatggctgg cgaataaacg
    17821 aatgttgttg atggtgtttt tcatgatctt tctccttttc aatggttgat tagagtgctg
    17881 ctaatttggc agcgaattct tgcagcgagg tggcgcggat ggtggtgatc ttctcctcgg
    17941 tttctttgct gagattgaat ttttcaccgc tgtagccgct atggatggct ttggaggggt
    18001 cggaaagcag catggtacag aaattgcggt tgatgacggc agcggagagc agtcggctgc
    18061 attccatatc gtgggctggt ttgctgtagc tgtaattttc gattttctta agcatggtga
    18121 atactcctaa taaggttatt ggttggtgat cagcgaccga tgccgagggt ggcgttgggg
    18181 gcgccggcag ccaggacgaa taaagcgatg gtgaggatgg cgaggacgag ctgtcccttg
    18241 tcgccgacga acaggttggc tttattggtt aggttcctca tgatttttct ccttttttga
    18301 ttagatattg gctacctgag tggcgaaatc caccaggctg gatgcatgga tggcttttat
    18361 ttttgccaga tcctgcgcat tcacatggaa gtattcgtcg tgaaatcccg cttcgatggc
    18421 gcgtagcggg gactctaaaa gcatacgaca gaagggtccg ttgatcaccg cggcacaggt
    18481 gatgcggctc atttccgtat ttttttctgt ttttgtggga agaatgggcg tgaaattaaa
    18541 tgtcctgagc attgtgtgcc tccattgact tgcgttgaga ggaaccaccc aaggaccaaa
    18601 ggaacaggaa tgccccaaca ctgataaata tatcaccaat gctgaatgcg acccggtagt
    18661 tcatccattg aggaaggaca aaaatgtctg aaaggaacca caacctcgtt ccttcaaccg
    18721 gcagcaaaat atcttttcca aatcccaccc gctcgccgat ggtgagcgca acatctaccc
    18781 caggaggggt caatttctgc gcggtttccg ggcttaatgg cataaaacca tggttgagga
    18841 agatcaccag gaaattcaga aataagccac atcccaaagc ccacatgccc gcttctgtgc
    18901 gattcaacca tacgaagacc atcaacaaga tcagggatcc gatcaaaatg aacggcatcc
    18961 aggaatcagg gatcctttcg cgggtaaagg acagggaggt ggctaaatat tgaggaagga
    19021 aagcagcgaa aaccaaccag acgctccgca tctttaaaga tcgaaatttc tcatgccgaa
    19081 tccacgatcg gatgagagca gcaatcaaac cggcgacgac agcgaccaag aggaccatga
    19141 tactagatta ccctcatttt caaataatga gcacctacgc agcccagatt tctctgatga
    19201 attttgtgct cttgtgcttc tcagtaaaga ggtgttgcca gccttcgtta gcagtgcccc
    19261 ctacgtaggg ggcactgcta ggcgttgagg taattgttcg gagggcattt gatcaacaac
    19321 ctcaattctc tgtctattat ggaattgtca agactgcaag agtggcttca aatgcgcatg
    19381 agtatttcac atatgtgaat tttcattatt atgggtattt aaaacaaaaa tctttttttc
    19441 aattagaata gatccattca aaaccattct catgtgaaaa ggaaacgacc atgcaaaaaa
    19501 agataccgct ttggacaaag gttgtatacg gatcgggcga ttttggatac agcatgacca
    19561 attcaattat tgccgcctat tttttgcttt ttttgacaga tgtggttggt atgtctccgg
    19621 cggttgccgg cattgccatt ctgatcgggc gtacctggga ttatattaat gacccgctca
    19681 tcggaaatat ctctgaccgc acgcactcgc gctgggggcg ccggcgaccg ttcctgcttt
    19741 tcggggcggt gccttttgcg atcaccttcg ccatgctgtg gtacaaaccg gcttttaccg
    19801 atcaaaccgc gctggctgtg tatttcgctc tggcatatgt gctgtatgat gcggcacaga
    19861 cctttgtatt catgccctat tttgccctca ccccagatct gaccgatgac tatgacgaga
    19921 ggacttcgtt gacgtcctac cgcatggtgt acaacatcat tgggggtctg gtggctttca
    19981 cattgccgct aatgctggtg aagagctttt cacctgatca tgccaaccgc gttttcacca
    20041 tggggctttt gtttggattg ctgagcgccg ttccgctctt ttttatcttc ctgaagaccc
    20101 gtgaaaaaaa tgccgtcgtg aaatatgaaa aacccgatat cttcaattcg ctcaagctgg
    20161 cattgaagaa cagacctttc gtgctgggag cggtggttta cctggctacc tggacttcta
    20221 tgaatgttgt gcagacagtg ctgctctact atgtgaaata cgttttagag cgagaagcga
    20281 tgagcgatat tttgatggga tccatcttca tcacagccat catcgccctg ccattgtgga
    20341 actgggtttc caaaaaactg gataaacgca aggcgtacgc gctcggtatc tcgttctggg
    20401 caatcgctca attgttgttg atgagcttga gcgcaagttc ttccactgtc tgggtgatgg
    20461 ctttatgcgt cattgccggt gtgggagttt cggctgccca tgtgctgccc tgggcgatga
    20521 tcccggatgc cattgaatat ggcgaattga gcacggggga gcgccacgaa ggcgtctttt
    20581 actccctggt cacattgatg gcaaaagtgg cgtcgtccat tgccatacct ttgacggcat
    20641 tcgttctgga aattacccat tatgtgccca attcagccgt gcaaccgcaa actgctttga
    20701 acggcatccg ctttttgatg ggacccatcc ctgccattct gcttatcatt ggcattgtct
    20761 ttgccttgcg gtatcccctg gaaagaaaag aattcaatgc tattgtggat aaattgaaca
    20821 aaaaacgaac gtcgcagaaa gaacttctgc agtaaagtat cttgaaatct cgtgagtcca
    20881 taactatccg gttggttgtg gtggatggca gttcattata aaatggcttc acaatcgcga
    20941 aatgtgcgca aagagagaat atggcaggtt ggtgttctct cttttttctt tctcttaatc
    21001 aatgggtgca gtctgttttt gccgaccgct gagggaggaa cagccaccct ctctatagag
    21061 gaacaggtcg ccggtaccct ggctgccttc caatcaaccc ttgcgctgac cttatccggc
    21121 accgcgccgt tggttgagcc tggaaatgat ttctcgctgg aggtgaccca ggaagtgttc
    21181 ctggctactc ctacagcact ggctgatccc ggggatcagg aattgcccac ctaccatcac
    21241 atctacaaca tttgggggca tcgccagtac tttgcgatcg gctgcgaagc tgccaccgcc
    21301 accgactggg cggcgttctt tggcgtggtg gtcaatgaat tcaattttca gtatcaattg
    21361 ccccagtcag acaatcctga cctgggtttt gtaggcagcg tggaaggacc ctggggtcag
    21421 gttcccccat atgcctatgg ggtgcatgcc gcgcctgtgg caaaagtttt acgagaagat
    21481 tatgggctga acgcgcaggg ggtgaaaggg tttactcttg aagaattgaa agccgaaatt
    21541 gcagccaatc gcccggtgat cgcctgggtg gtcgggaatt gcgtgggtgg tgtgccctat
    21601 gaatacaccg attcacaggg caacaccacc attgttgctg cgtatgagca tgtggtcatt
    21661 gtcaccggat acaccgaaga caccatccgc tacatgaaca acggaaaatt ctatgatatt
    21721 cccacggagt attttctaaa ctcttggggc atcctgggaa atatggtggt ctaccttgaa
    21781 accggttgat cggttacgct tattcttctc tgaagagcat attgacgaag atggaaacag
    21841 cgctcacgat caaagagccc aacagcgccg cccaaaagtc atccacatag aacccgacgt
    21901 tgaaccagtt taccgcgatg ttgctggcag ccatgaagga taccgcgttg atgatgatca
    21961 cgaacaatcc caaggtcagg atgatgagcg ggcaagacag cactttcaaa ataggacgga
    22021 tgacggcatt gaccagaccc aggatgattg ccatcacggc aaaaacctgc caggcttctg
    22081 ggctatccac gtggattccg ggtaccagat aggcggcggc gaacaacgcc agcgaggtag
    22141 ctgcccaacg gatgatcagt ttcatttttc gctccttaca aatttctaat gtagaaagtc
    22201 ctactactat tctataatag tcaaaaatat aacgaaatac tacgtaaaaa gttttcttgt
    22261 ggagttccta tgcattccga tcaaaaaacc cgtctcaagg acctgccgcg caatatctgg
    22321 gcagttagtt taaccagttt ctttatggac atatccagcg aaatggttat caatgtcctg
    22381 ccactttttc tgtccaatgt tctgggagtg aagacgaaca tcatcggctt gatcgaaggg
    22441 gtggcagaag ccaccgccag cttgctgaag atgatctccg gttggctttc tgataaactg
    22501 aaaacgcgaa aatggctggc tgtgctgggg tatggcatct ctgctctggc taaacctttt
    22561 ttctattttg ccggcagttg gggaagcata gctgccatcc gttgggctga ccgggtaggg
    22621 aaaggggtgc gcactgctcc gcgcgatgct ctggtggctg attccattga agaaaagcaa
    22681 agaggtctgg cgtttggctt tcatcgggcg gctgatacct ttggtgcggt gctggggtta
    22741 gcggtggcgg ctttggtcgt ctggttgacc caacgctcct caggcatgct caccgtggac
    22801 accttcagga ttatcgtttt gctcagtttg atccctgctg tgttgtcggt tctggcattg
    22861 atccttggaa cgaaagaagt agaaatcaaa ggagcccatt ctctgccgaa aatcagcctg
    22921 aaagcgctgg gaaaggattt cttgatcttc ttgctcatcg tctcgctgtt cgacattggc
    22981 aattcatccg atgcctttct gacgctgcgc gcccaggaaa gaggcgtgag cgtgttgggc
    23041 attttgatca tgttgatcgt cttcaatctg gtttataccc tcatttccac ccccgccggg
    23101 atcctctctg acagggtagg gaggaagaag atgatcatcg gtggctggtt ggtatacgcc
    23161 gcggtatacc tcggtttcgg attggcgcaa accacctggc atatctatgt gttgtacgtc
    23221 atgtatggca tctattatgg attgacctat ggcaccacga aagccatggt ggctgatatc
    23281 gttccggttg aattgcgcgg aaccgcatat ggcacctata atggcatcat gggaatcttg
    23341 gactttccgg cttctctgtt ggcggggatc ctctggcagg gtgttggcaa atggggcgga
    23401 ttcggtcctt cggcaccctt cctgttcggc gcaggcacgg ctttcgcggc tacaatatgg
    23461 atggcagtgt gggttcctgc cacaaaaaag actgacaatg gttaaaatta gaccaataca
    23521 aaaataatgg tagcatagga caggaacatg caagtaatta ctaaaaagaa caaatatttt
    23581 attggtgttg cactgtgtgt gatcgcggct ttcagtttta tcaacggcga ttactacagc
    23641 gcggtcgctt tactctttgt ttgtgtgagc gcgttgacga ccattgacaa aaatgatgcc
    23701 tctgtaaaga atccgcaggt ctggcagggc ataaacctgg ctggttatgt tttggcagcc
    23761 gcgatttgga tctacagctt ctatatgaaa aaatagattg aagaaacgta tgcagtggaa
    23821 attgggtgct tatcaagtag ggaacgcatg gagatcgcag caagacgtgt tcaaactgct
    23881 ggtcacccat ttccgccgct accatctcgt ggagatccag gatgtctaca aactgatcta
    23941 ccagggcgta cgtgggtctt cccacatctt gtcttcatca ccttcagatt ttgaagtgaa
    24001 cctgcgtagg gagtttgctt ctgttgaagc caataattcc cagccattat gggaatctat
    24061 ccatccagag ggaaagatcg tgcgggtgaa cctcgcggct tataagtatc aatcacaaaa
    24121 gatcgagcgg ctctccgcgc tttgcctgtg gacggctgaa atctccactt catcagagaa
    24181 ggatgtcgaa cacatttcga ccgcctttaa cactttctac aagttatgca aaagccgcaa
    24241 gatccccaag ttcgctccgt tggaggtcga gaaataccat aagtgggtgg aagagaacaa
    24301 atatccggtg gtgcatcatt ccgaaaccta tcggacggaa tatgatccgc attatcgtct
    24361 ggtgcatcgt gattttctgt ccgttttatt ctagaggtcg tttgccgtct gggtgatcgc
    24421 ctgggctgcc agtatagcag aaaggaaagc ccagtgcagg ttgaaccccc cgcaggatcc
    24481 cagcacattc acgatctcac cggcgaagaa caagccactg caaactctgg attgcatggt
    24541 gagggggtcg atatcgcaga gcgccactcc cccactagat gcctgggcat atttgaatcc
    24601 gcgcaccccg cgcacgctga gcggctgttc ttgcatggaa tgcaacaaac gctctttttg
    24661 ctcgatggtg agttcaccgc aggttgtcac cgattccata tgggtctgtt tcaagaagaa
    24721 atcggcaagt ttggcaggta gatacccctg caagacggca gccggagaaa gcttgtgcag
    24781 ggcgggatct gataacgaat cgcaaatggc atggctgctt ttcgggatga aattgatctt
    24841 cagggagtat tcttgtggtt tttgcgggtc gatccagtgg ctgatgtcca tgacccccgg
    24901 accgttgatg ccccattggg tgatgatgac gttccccagg gtgcgggtga tctctttgtg
    24961 atggtgaagc aagcacacct ccgcatccag gcgcaccccc tgtagtttgt ggaaaagctc
    25021 agggtcagtt tcgatgggcg tcagggctgg tctgacaggc agaatatgat gccccaacct
    25081 ccccagcgcg gcatagagtt ccccgcgcgc cccaagttgc ggattggcag gactgccaca
    25141 ggcgatgagt agcgtcttca catggtgcac tttgccccgt tcggcggtca gcaacgcaaa
    25201 atgatcttca tttttctgaa tatccgtgac cagtgtgttg tgaaaggtag aaacgtgctg
    25261 caaatggttt tccatgatgc tgaccacatt ggcggcggaa aaagagagcg gataaatcca
    25321 gccgtcgtcg gtagttgtgg taggtatgcc gatcgatctt aaaatttcca gaacccgcgc
    25381 gttggcaacg gttcgaaaac actgatgcaa tccttcagga tcatcggtga aataggcatc
    25441 tgcttttgcc gagcgattgg agatgttgca gcgtccggca cctgtggcgc tgagcttgcg
    25501 tccaaggctc gcgttggcgt caaggagcag aggtttaagt ccctcttttt gtaaaaggat
    25561 ggctgccatc atcccggcag ccccgccgcc tacgatacca atctgcattt ctttctctcc
    25621 attcttgtta tacgataaga agaaacggta aaattcgcac aaaattgcat aatgtattag
    25681 aattaggcaa tttcgttaat ttttcaggag gttagcatgg aaaaacattt gtatctgtct
    25741 ctcttgcctg aagcccttat tgtttcaatg ttaacaccag aagagtttgg tgtctattat
    25801 gcagtcgggt cgttaaaaaa atcgcgtggt caggcgattt tttttgaaat tgaccccgat
    25861 ttccgatctg atgagtttca aattgaagaa gcgttaaaac gatgtgttcc tcatgaagat
    25921 ggcaccccca agcgctctct gtacgtttct gtctaccgcg tattggaacg tattccattg
    25981 aagactttgc ggaagcttta ccttgtcaca caagatggtc ggacgctggg tatcgaaaaa
    26041 agcgtacaat tgccaaaaga ggaagagggg atgcacctct accgggaaat ttccccggtc
    26101 aatccgctgg ttgtcagcac cctgaacccc aaagctttct acgaattgat cgttcgcaat
    26161 cccaccagcc tcatcaagct tcccgctgtg tgttttgctg agctgaaact gggggcgttg
    26221 gcagatgatc cgcaaaatgg cgacattggc gacctgccct atgaaaacat ttaccatatc
    26281 cgtgactgcc tgttggaact gcgcacaaaa tatgttcatt ccaaaatggt agatcgcaca
    26341 ccttcggggg gcattcctta tcgcaccatc aagcatggtt tttttgtcgg caatgaaaaa
    26401 gagcttgtgc attaccccat ccccactcag gaagaattga ttaaagaaca ttattcctgg
    26461 tggcgatccg caagtttcta aggctagcat tgagtgggtt tactagctgg gattcccaga
    26521 tgggtgatgt atggattttt ttaaactaat tttatggaag gagtcattat gtcttcgcaa
    26581 agctggttgt tgataggggt gggcgcggga ttggtatccg tggctgtggc gctgtatctg
    26641 tactattggg tgaagaaaca gaatgcaggc agcgcaaaag cgcaagaagt ggcgcgctgg
    26701 atcagggaag gggcgtcttc gtatctgaag aaattgtata cgtccctggg tctggtggcg
    26761 atcatcttgg gctttcttat cgcgctggtg ttcagcttcg acctggacaa tttgggaact
    26821 tctcaaatca ctgtcgacag cgctgaaggg ttgcgtattg catttacctt catattcggt
    26881 gcaatctgtt cggcggttgc cggttatttg ggaatgagcg ttgccgttga agccaatgtg
    26941 cgcagcgcaa ctgccgctga aaaatctttg aaccagtcct tcagggtggc attctatgcc
    27001 ggttccgtga tgggtctggc tatggtcggg ctggcggtgg ttggcatgtc cacaattttt
    27061 ctcatcaccg gcgatccgga attgatcctg gggttcagtt tcggcgcttc taccctggca
    27121 ctgttggcaa aagccggcgg cgggatctat accaaaactg ctgatatcgc agcggatttg
    27181 gtagggaagg ttgagatcgg tatccccgaa gacgatccgc gcaatccggc ggtggttgcc
    27241 gataacgtgg gtgataacgt gggtgacgtg gctggcatgg gtgcggatat tttcgacagc
    27301 tatgtagcca gtgcagtggc tgccatcctg ttgggtgcag cattgcccaa ccaaagcgat
    27361 acgcgctatg tggtgttccc gttgatcctg tgcgccctgg ggattgttgc ctcattgatc
    27421 ggtattcaat ttgtgcgtgt gggaaaagat ggaaaacccg gtgcggcttt gaacagaggc
    27481 accgtaatca ccgccatgat ctatgcattg atggtgagcg gtttggtact ggctggtgac
    27541 tatgacaaag gcgctctctg ggcgactctg gtcggtttgg ttaccggcgt ggtcattggg
    27601 ttcacttctg attacttcac ttctgatgca cacaaaccgg tgcaagagac ggcgagagtc
    27661 tccggttccg gctcggctgt caccatcatc actggtttta gctacggctt gatcagcatt
    27721 gtcccttcgg tcatcggcat cgtggtggca accctggctt cctatttcct ggtggaagct
    27781 tctggcactt tgagtggcgt atatggcatt ggcatcagcg cggtgggaat gctggcgatc
    27841 agcggtatga tcgtctcggc ggatgcttat ggacccatcg tggataacgc ccgcggtatc
    27901 gctgaaatgt ctggcatgga tcatgaagtg attgaacggg ctgacattct ggattcggct
    27961 ggtaatactg ccaaagccat caccaagggc ttttccatca gcgcggcggc tctgaccgtg
    28021 ctggcgttgt tcgcatcgta tgcagaagcc attgaagcca gcggttatgc actgacgttg
    28081 agtctgcgcg acccgatcgt gatcggcggc gttttgctgg gcgccatgac gcccccgctt
    28141 ttcagcgccc tcaccatgct ggcggtcacg cacaatgcct ttgacatgat cgaagaaatc
    28201 cgacgtcaat tccgcgaaga acccggcatt ctggcaggca aggtcttgcc cgattacaac
    28261 acctgcgtga gcctggcagc ccagggtgcg ctgcgctctc tggtcttgcc ggctttcatt
    28321 gcggtcggtt tgccgctatt ggtcggtttc ctgctcggac ctaatgcgct aggaggcttt
    28381 ttgggtggcg cgatcttcat cggcattgtg ctggcgctgt tgatgtcgaa cgccggcggg
    28441 ttgtgggata acgcaaaaaa atatatcgag tcgggtgtct ttggcggtcc gggttccgaa
    28501 gcgcacaaag cggctgtggt gggtgacacg gtaggcgatc ctttcaaaga taccgctggt
    28561 ccgtccatta acacattgat caccgtgatg tcgctggttt ccaccctgtt tgcgccggtc
    28621 atcgctgcct tccatctctt ctagaaaata atcaacaaat aggaaacagc ccctgaatga
    28681 atacataggg gctgttttta ttggcggtat cttttttccg atcgtttgcg ttcaatgacc
    28741 ggcgcgattc cgccaggacg atgatggctc cggctgtcag gtcgaagaaa tacgaaagat
    28801 gtactcccgc aggttgaagg gggtgttctc cgcagtgata aatttttcat cttcgggtat
    28861 ttttttacca gacggttgag ttttttgtga aatttccgct agaatggggt tatgaccata
    28921 ccgattttct cccatacaga cctgtcgtct ctttcggagc gaacaaaacg ccatttggaa
    28981 agtattacaa aaagtacgaa agtcccaaaa ggggaacagt tcttgtggga gggtgatgaa
    29041 gttgaaaaaa tctatttcat cattcgcggt tatgccaaga tctatcattt ttccgatgac
    29101 ggacgggaac aaatattagc catattagac agcgggcaat ggatcaacac gatccctgct
    29161 ttacggggcg agcaaggaaa tcacgccaat ggggtggcta tgacggagat ggaactgggc
    29221 gtcatggcag caagggattt tcttgatgcc atccgtgaat tctccgattt cgcgttgttg
    29281 atcctgcacg atgtggctga taagttggat catatgacca atctggtggc agagctatcc
    29341 ctttatacaa cgcgccagcg cctgatccat tttttgctgg agcactgcaa agataagaat
    29401 acgatccatg gctggacgca ggaagagatc gcatcacaag tggggaccgt gcgagacgtg
    29461 gtcagccgct tgctgggtct tttcgcgcgc caggggtata ttcgcgtgga gcggcagcgc
    29521 atcatcgtgc tggatgaggc aagcttacga aatgcattga atgaatgaaa tgtgcgactt
    29581 aagtcataga agggattgcg ctttcttgat acgctataga tcatggagac caaaatgacc
    29641 tggtttttac ctgtgatcga tgaaaagaaa tgtgt
//
LOCUS       DAOSDS010000017        29507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_750_length_29507_cov_4.267045, whole genome shotgun sequence.
ACCESSION   DAOSDS010000017 DAOSDS010000000
VERSION     DAOSDS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 29507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29507
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_750_length_29507_cov_4.267045"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..901)
                     /locus_tag="PLL88_03655"
     CDS             complement(44..901)
                     /locus_tag="PLL88_03655"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOJ00688.1"
                     /translation="MSKVVVVTDSTAHLPENLIQELDLRIIPLHVVWGDKVYQDNVDL
                     KSENFYTMLKQAKDLPTTSQPSPKEFMDLYKPILDAGDKIISIHISSGISGTVNSAFQ
                     AKEMLGSQDIEIVDSKSSGMVLGLIAVLAARRARENASLKECKELAEKAVANSNVFFM
                     VNTLEYLHKGGRIGGASALLGSALDLKPILYFKDGKIESYEKVRTKKKAINRIIEIAR
                     EMIGAKRPVHLAILQVEAEEDAEYVKAELKKLYSPQEIQDLLITSLSPVIGTHTGPGV
                     IAICYIAGV"
     gene            1107..1436
                     /gene="rpsP"
                     /locus_tag="PLL88_03660"
     CDS             1107..1436
                     /gene="rpsP"
                     /locus_tag="PLL88_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOJ00689.1"
                     /translation="MVKIRLRRTGLRNQSSYRVVVCDKEAPRDGRFLEIVGFYNPRTE
                     PFTFDVQEGRVYYWLSKGAQPSESVAKLFKTVGLTERFEKVKAGESEETVLKEANAFY
                     ESRKAKK"
     gene            1479..1712
                     /locus_tag="PLL88_03665"
     CDS             1479..1712
                     /locus_tag="PLL88_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOJ00690.1"
                     /translation="MKELVEYIAKSLVEDPTQVSVNETEEGDHVRIELTVAKEDMGRI
                     IGKGGRVANSIRTLLRVAAAREGKRASLDVIEP"
     gene            1712..2266
                     /gene="rimM"
                     /locus_tag="PLL88_03670"
     CDS             1712..2266
                     /gene="rimM"
                     /locus_tag="PLL88_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOJ00691.1"
                     /translation="MAESPSNSKNEINNGSPEIGEPLFLIIGKIRKPHGVRGEMLVKL
                     LTEFPERIEEGKRVYIGESKTEHTIATVRFLHEGCLIKFSAVNSPEQARQFCNEMIYV
                     RAVNLPKLPENQYYYHDLLGMQVKTDEGEMLGTVHEILETGANDVLVVRKGEQEHLFP
                     FIKQVVLDVNQRENYILIKKLEWK"
     gene            2268..3371
                     /gene="dprA"
                     /locus_tag="PLL88_03675"
     CDS             2268..3371
                     /gene="dprA"
                     /locus_tag="PLL88_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOJ00692.1"
                     /translation="MDEKKYWVGFNHVRGIGAVRFQRLLECFGNLEKAWNAPEIELKR
                     AGIGDKSLVNLVRFRREHDLDHIYENILKKDIKILTCMDDDYPQQLKQINNPPPVLYI
                     KGEMVREDAHSVAVVGTRQITAYGKSLVQELGRVLAQNHITVISGLARGVDAEAHRTA
                     LEANGRTIAVLGCGIDIIYPPENRALASQITEHGALISDYAPGTQPEGINFPPRNRII
                     AGLSLATIVVEAGARSGALITASFAADQGKDVFAVPGSIYAPQSKGTNKLIFDGAYPL
                     IRFDSIFEILGLDNVQYQKKTQSELPTDEAQLLVLKILQKEALHIDDIQAISQLPIER
                     VSSALSFLELEGFVQRTGNMTFSAIYDFKEEYS"
     gene            3373..4461
                     /locus_tag="PLL88_03680"
     CDS             3373..4461
                     /locus_tag="PLL88_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOJ00693.1"
                     /translation="MHDNYFAVIMAGGGGTRLWPLSRQLKPKQLLKPFGERTFFQMAV
                     DRLSPVFTPNHILVVTVASHVAELRQQVDSIPLENYLIEPMPRGTASVVGYAAEVLQQ
                     TYPDAVMAVLTSDHIIENVPLFHTLLENAYEVAQNGFLVTLGIQPTFPSTGYGYIHKG
                     EKLSAFPFSAHLVQSFVEKPDLECAKQYLAAGSYYWNSGMFVWNTKNILDEFNRQMPV
                     LYSSLEVLGQRYGKEGYETLLAETWEKITPQTIDYGIMEHARQVAVIPAENLGWSDVG
                     SWDSLFEIIQADDNGNINLADSSIIQESSNCLIKTDNDKKMIVLAGLDDIIIVDTRDA
                     LLICRKGQSQRVREIVNELKEKKLDQFL"
     gene            4472..6775
                     /gene="topA"
                     /locus_tag="PLL88_03685"
     CDS             4472..6775
                     /gene="topA"
                     /locus_tag="PLL88_03685"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOJ00694.1"
                     /translation="MEAYCVKCKEKRELQNPVAGFNKRGAPITTGTCAVCGTKLVRIG
                     KTEAHVGLEPPKNEKKEVRSEKLVIVESPAKAKTIGNYLGKEYKVKASVGHIRDLLRS
                     QLSVDVENNFAPKYRVPNEKRPIVKELTKDVQSAGEIYIATDPDREGEAIAWHLKEST
                     NMDESRVHRVVFHEITKPAIQESFAHPKNIDMDLVNAQQARRVLDRLVGYNLTPLLWQ
                     KVRGRLSAGRVQSIATRLIVEREREIDAFVPQEYWSVEVEFKPENLKKTYLAQLSLID
                     GKELTLSSQAAVDEILEDIKKASYVIGKIKKGERKRSPSAPFITSTLQQQAARQLNFR
                     ARRTMMIAQQLYEGIPLDGGSQTGLITYMRTDSTNISPIALNEARQYIQSVFGDSFLP
                     SSPRLYKTRAAVAQEAHEAIRPTSVFRTPEKVKDFLSRDQYRLYQLIWKRFVASQMEN
                     AIYDTISVQVDGNGDKHQYVLRASGSKVRFPGFLTLYEDAREEDQKDGEKNIPIPDGI
                     FEGQKQILININPQQHFTQPPPRYSEATLVQALEKNGIGRPSTYAPIISTIQTRGYVE
                     REGSKLKPTEIAYTVNDLLVEYFPDVLEINFTAEMEQDLDEIANGAKDWVDVLKTFYG
                     AFEPRLEHARADMPEMNAEPEKVGKACPRCGHDLIIRWGRYGKFISCSNFPECRYTEA
                     YLEKIGVTCPKDGGEIVLRKTRKGRIFYGCEHYPNCDFTSWKRPVSTPCPACKGLLTI
                     KNNKELVCADCGNTFSTDILESQEEMA"
     gene            6835..7863
                     /locus_tag="PLL88_03690"
     CDS             6835..7863
                     /locus_tag="PLL88_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00695.1"
                     /translation="MKNVRKTYEKPFFWPVVTLILGLVLGLVVLGWWLWPVEWVDASP
                     SQMQNEYKRDYLCMVIDSYVRNHDEDLVNKRLYALGEDAEAYLNMLTPELCKFTSAEE
                     ITSFRALKFTDSTVTSNTITEQAAELTKEQADGSKKTSLGNILLIAALVLLVGGGAFY
                     ILVKRGKINLNRQVDKRREVNKGFEVASPVVEETAQASQEPPLAQFMTTYRLGDDLYD
                     DSFSIDSPSGEFLGECGVGIAETIGVGDPKKVSALEVWLFDKNDIQTITKVLMSAHVF
                     NDTAARQRLAAKGETILVEPGQRFIMETATLQLEARVVDMVYARDALPENSHFSRLTL
                     EIAVWSKM"
     gene            complement(7888..8355)
                     /gene="greA"
                     /locus_tag="PLL88_03695"
     CDS             complement(7888..8355)
                     /gene="greA"
                     /locus_tag="PLL88_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOJ00696.1"
                     /translation="MADTVYLTPEGVQKLEQELAELKGPARVALAKRLKAAIEQGDLS
                     ENADYSKAKEDQAFTEGRIQEIEAMLKNYIVIDGKQNVHDTVEIGTTVTIQESSDSPE
                     TYFLVGPAEADPAHGKISFESPIGSALLKHKVGDTVKVSTPAGYLLFQILDIK"
     gene            complement(8405..9202)
                     /locus_tag="PLL88_03700"
     CDS             complement(8405..9202)
                     /locus_tag="PLL88_03700"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HOJ00697.1"
                     /translation="MLVFVKLGGSLITDKNQACSARGVVISRLAQEVSQALQDDPALQ
                     IILGHGSGSFGHPPAKKYQTRSGVHSAEEWCGFIEVRRQADALHRIVMDELWQIGLNA
                     ISFPLSAVAITKNHAVVQWDTAPLHNALDHSLLPVVFGDVVFDLEIGGTILSTEEILA
                     FLCLHLPIQRILIVGIEQGIWKQADSPANIYPVFTPALFHAQNAGISGSRSPDVTGGM
                     RSKVEVLFSIINRNPAIQASIFSGKEEGNLYQALRGAEVGTVLKDHQ"
     gene            complement(9205..10146)
                     /gene="mvk"
                     /locus_tag="PLL88_03705"
     CDS             complement(9205..10146)
                     /gene="mvk"
                     /locus_tag="PLL88_03705"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HOJ00698.1"
                     /translation="MPAIVVHTVGKSILFGEHAVVYGYPAIAVPIPALKTTVTIFPLI
                     GKEQQVSIYDAPDLAISAKLSDLPENNVLVHAVKLFKDAYAISTIPSFRLKIKSHIPV
                     ASGLGSSASVSVGVVKALSQFLGLKLPVQKINDFAFELEKLHHGNPSGIDNTVISYEK
                     ALCFTKGLEPEFLQSPTPFHILLIDSGIASHTVEVVSAVKQARENDPQTINSIFQEIG
                     EITQSAKKAFLRGNAQELGGLMQRNHALLQALHVSLPELDTLVSLALNNGAWGAKLCG
                     AGRGGNVIALVGEENAPFIQQALVSHGVKGIVHTVIQ"
     gene            complement(10152..11039)
                     /locus_tag="PLL88_03710"
     CDS             complement(10152..11039)
                     /locus_tag="PLL88_03710"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HOJ00699.1"
                     /translation="MLKAIIQSMRPKQWVKNVFILAALVFDRQLTNWSATLTTLIGVF
                     IFCMLSSAVYLINDVFDKKADQSHPTKKERPIASGRLPTNVAIPVAVLVVVGSLYGAF
                     RLSTSFGVISAVYFVLNLAYSFKLKHIPLIDVMVIAAGFVLRVAAGVSLIQVERFSPW
                     LYICTSLLALFIGLGKRRAEIICLSDNATQHRKVLDGYSLEFLDQLITIVSGTSIMAY
                     SLYTFSAPNLPENNTMMLTIPFVLYGLFRYLYLIKVRKSGGAPEDLVLSDRPLQVCIL
                     LWGLSVIVIFYWAKIIALF"
     gene            11095..11910
                     /locus_tag="PLL88_03715"
     CDS             11095..11910
                     /locus_tag="PLL88_03715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOJ00700.1"
                     /translation="MQKKRLDVLLVEKGLAESRSRAQRLIMAGEVMVNGVQRLRPSEI
                     YSDEIAIALRETAPFISRGGEKLEASIQAFGFSDLTGKVCADIGASTGGFTDCLLQHG
                     AEKVYAVDVGYGQLHWKLRNDPRVVVMEKTNVRNLKTLPEKMDMVVVDVSFISLRAIF
                     PVLKGLMKESEAAMIALIKPQFEAGKQEAARGKGVIRDPVVHKKVLENVLQAAQAQGL
                     YLHGLIPSPIKGPKGNIEFLALFRAEPGQENLEQRIQQVLEKEVGTAEPQETQ"
     gene            11939..13738
                     /gene="lepA"
                     /locus_tag="PLL88_03720"
     CDS             11939..13738
                     /gene="lepA"
                     /locus_tag="PLL88_03720"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOJ00701.1"
                     /translation="MQLTQKRNFCIIAHVDHGKSTLADRLLQMTGVISERDMVNQVLD
                     NMDLEREKGVTIKASAVRMEYKKNGLIYELNLIDTPGHVDFSYEVSRSLIACEGAVLV
                     VDATQGIEAQTLANLYLAIESDLVIIPVLNKIDLPSAHADEVAEDVGNLLGCDPDAII
                     RISAKTGLHVDQVLEAIIERIPPPKGDEHGALKALIFDSHYDSYKGVIAYVRIFNGKA
                     DMSGKFLLMSNHVETDPIEIGIFAPTMKPVDVLTAGDVGYIATGLKTVKDCRVGDTLT
                     DKNNPAEHSLPGYKHPKPMVFAGVYPVEGEDYDNLKDALAKLQLNDASLVFTPETSQA
                     LNFGFRCGFLGLFHMEIFQERLEREYDLDIVLTAPTVEYKVILHSGEEVLIDSPAMLP
                     DESTIQEIQEPWMNLSIFTPTEFYGAVMELVKRRLGVFSSQEYPSPNRVQLSFEIPLS
                     EMIVDFFDELKSKTRGYASMDYSFKEYRASNLVKLEVLIGGEPVDALASIVHKDLAYH
                     LGQRLVTKLKELIPRQLFDVAVQASADGRIISRASIKAMRKDVLAKCYGGDVTRKKKL
                     LEKQKKGKRRMKMVGNVEIPQEAFMAILKLEDE"
     gene            13758..14750
                     /locus_tag="PLL88_03725"
     CDS             13758..14750
                     /locus_tag="PLL88_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOJ00702.1"
                     /translation="MKNSWKRWLPGVVVSAIVIYVLYRIIDIPIFLSAIRKTNLFYGM
                     VALCLMVLANISRAAAWRELLGRKISLRDAFHIVNEGYLLNQIIPRSGEVGRALLVNS
                     VVEMNFFEVFSTIIIERAFDLGITAVMFLSTIGKAIAMDWIVPVAITILGIVLAGFLF
                     LFWAIKKKTAVESWCDRMDQKSVFFRKYVSPNIKAIIRGAQIMQDPSRLLLAFLLIAV
                     CWLLWMSISFILLSSFVGVKPFWWVVFIQSVLSLGIALPSAPAGLGVYEGTLVAALAA
                     FAIEKETALGFAIIMHAVQLVAIAVLGIYSLAKQGNSLGLLLNKVTNRLRKVKE"
     gene            14753..15775
                     /locus_tag="PLL88_03730"
     CDS             14753..15775
                     /locus_tag="PLL88_03730"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ00703.1"
                     /translation="MSYQYLITGGAGFIGSNYAHRLLLKGEDVTIFDNLSRNGTERNL
                     EWLYQQHGKKAFTFIQDDIRNATAIEKAADGKQVIIHLAAQVAVTTSVAEPREDFEVN
                     AQGTFNVLEAARKSGNQPILIYASTNKVYGGMEEVKVKEEPTRYSYVDYPHGISEDYP
                     LDFHSPYGCSKGCGDQYVRDYQRMYGIRTVVFRQSCIYGNRQFGIEDQGWLAWFVIAA
                     AQGKPITIYGDGKQVRDVLFVDDLVDAYDSAIQQIEKASGQVYNVGGGPANTISVWQE
                     FGALLEKKLARKVPVSTSNWRPGDQKIYISDIRKAGQQLGWQPRIAVEEGIARLVDWI
                     ASIETE"
     gene            15772..16968
                     /locus_tag="PLL88_03735"
     CDS             15772..16968
                     /locus_tag="PLL88_03735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ00704.1"
                     /translation="MTGMKILIELTYYRPHTSGLTIYAERLAKALAKRGHQVTVLTSQ
                     FDKKLPRDEWLEGVHVVRLPVLFRISKGVVMPSLGFMATKLVKENDVIHLHLPQIDAA
                     GIALRGKLWKKPTVLTYHCDLRMPWGVMSWLANQGVHMMNNLAAMFTDRIVTYTRDYA
                     DHSPYLKRFQKKLHIIPPPVELPVAAKQAIQDFARQHNPLGKRPIIGMAARFASEKGV
                     EVLLHAMMSVREVFPQAEVWFAGPYENIIGEEAYFKRLEPVIEKMIREGAWKFFGSLA
                     PAQMSLFYPNIDMLVIPSLNSTEAFGLVQIEAMMNGKPSIASNLPGVRQPVQRHAMGK
                     VVEIGNSKELAEAIIAVAENPGQFHGDGKQIKTIYAPDEIARQYEDLFFGIMAEIKHG
                     RKTKRS"
     gene            16943..17752
                     /locus_tag="PLL88_03740"
     CDS             16943..17752
                     /locus_tag="PLL88_03740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ00705.1"
                     /translation="MDEKRNALEKRDFLWENLKRLPYFRALLRAVESRFYENIPLNEP
                     VLDVGCGDGIFAETTFEEKITVGFDPALTSLREPANRKTYSSILCAAGAAMPFQSAFF
                     STVISNSVLEHIPDLDAVVAEIQRILKPGGLFVFCVPNDQLLKNLSISKWLDRMGLRG
                     LAKAYRTFFNTISRHHHAESFEVWQTRLENNGFKIEKWWHYFFPKQVHALEWGHYFGL
                     PSLVNKKIFGKWILLPFHGNPFIPEKRLRKLYNQDPVHLEGSYTFYIVRKD"
     gene            17753..19819
                     /locus_tag="PLL88_03745"
     CDS             17753..19819
                     /locus_tag="PLL88_03745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ00706.1"
                     /translation="MPEKEPSLLDYLRSLLEFQPDRYRHYLRMDETAAHVPTKAQSKK
                     GFPYRFAFLPVLALVCALGGQFLLEPAHRITPANVIFYGMAVVLVIVFILSNRAHFQE
                     KVDREKAWFVSQEIRVNVLFPATLFLAAAFWFFQDNQFTVLNVVLWIAGLLLFVLSVW
                     ERKEIRARSKKGSGLFWILMILVAGVSIFFRVYDLQEVPSEMFSDHAEKLLDVADVLD
                     GNTPIFFIRNTGREALQFYLTALIIRVFNTGISFLSLKIGTLLAGILTLPFIYLIGKE
                     LGGKWSGLAALFLTGVGYWPNVISRVALRYAFYPLFTAATFYYLIRGLRTRNRNDFVL
                     TGLLLGLGLHGYSSARFLPIVVTAIFLLYFLRLRTRQEKHNLIWMFVLVALTAFFVFL
                     PLFRYALENPLMFGYRASTRLFPVEQGYPDNPLLIFLSNFWNALTMFYANNGVIWVHS
                     IPNRPAFDVVSAALYFLGTLFAVIQSIKRKNWLTTAILVSIPLLLMPSILSLVFPSEN
                     PSLNRTGGAYVLAFVVAGYGWAQVVEIFLGSAPTKARKGVALLLTLVLACYCGMQNYD
                     LVFNQYRTQYDLNAWNTTQIAEEIGRFYREGGEVDDAFVVPYPHWVDTRLVGINAGLP
                     RKDYALWSQDFVTVQQEPGMYLFILKPEDVENIALLQDLFPDNILYTYVSDTPGKNFI
                     EMRVQN"
     gene            19838..25030
                     /locus_tag="PLL88_03750"
     CDS             19838..25030
                     /locus_tag="PLL88_03750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOJ00707.1"
                     /translation="MKKTLQSKRFHITLLLLILFMSGFLRLRGIQWDGDFHLHPDERF
                     LTLVETSIYPVDSLREYFDTSVSTLNPHNVGKTFFVYGTFPIFVVRYVAEWLQQTGYS
                     QVHIVGRYLSALFDLGVVLLVYCIAKKLFKRPFVSLLSALFYGLATLPIQQSHYFTVD
                     TFANFFVAVALYLAICIFALKEEEIQNEKKYASHDRRMHVIFGFMVGLAAASKINAVV
                     CAVLLPLADILRDYPELKQSPQRRNQTLINIALAAFVCLVTFRIFQPYAFQGPGFFGI
                     SLNAKWLDNLRELSVLSSGDINYPPSLQWARRPSYFAWQNLLQWGFGLPIGIPALIGL
                     LVMGWKIVRENWKPYLLLWIWIVLYGGWQSQVWNPTMRYMLYVYPALAICAAWSVVSA
                     VNWLFQEKKVFRIVLAVCLLAALLGGSVAWAFGFSALYTRPVTRVAATEWIYKNVEGP
                     INLQISGADEDFSQPLAYPHYIDLTPANPITFKFTPEQDETLSAVMIDKIQNYSEESD
                     DQALTVSLYEMANGTPVLLQSALVSLVDWNENNVINSQSVQFSSSPALQHDKEYLVKI
                     ELSDQSTELRLSGFIKTAAYDESSIHFTQTIFECAPSLSQMGIYTVQFTPFEDGALTG
                     ITFFRVVDWEGTENEKEIRVRIQSMDGDENSFVTATLKSAFKEKQDFRGAAYTIAFDD
                     PVLLRQGKVYALTLEADQGAGNVAIYGSKRLNESSWDDGLPLSMFVAYPFDENTGVFY
                     SDLNLELYNDDNAEKLERFYTSLEEADYIYITSNRQWGSTTQIPERYPLTVEYYRALL
                     GCPVDQNLQDCYRDAQPGMYDGELGFELVKVFQSEPTIAGITINTQYAEEAFTVYDHP
                     KVLIFEKTDSFDMMTVRSILGKVDLSKVIYLTAAAASKVPGDLSLSESEKEIQTNGGT
                     WSALFSSDSILNQSQFLGIVVWYLVLFLLGVVVYPSTRIIFRGLKEKGYAFSRLLGML
                     LLTFLVWILGSLSVPTERWLISAVFAGMLGVNGWLYLKNRKMIQQEFKENQRYFLQVE
                     CIFLAFFVFFTLIRLGNPDLWHEYKGGEKPMDFAYSNAILKSTIFPPYDPWYAGGYIN
                     YYYFGSLIAAVFTKCLGVVPSVAYNFSLSTFFAFAGTAAFGIGWNFQSKRLENQTGKK
                     RAFSPFQAGIVFAVAVLIIGNLGTISMILSGIKQIAINAGLQTGSGIFASFLLWCKGF
                     ILLLKGAKFTYYPGDWYWIPSRTIPGEAITEFPYFTFLYGDPHAHLYAYPITLMALGF
                     AVSIVFRQKKYDGQRDGEWLFLLLGGGLVVGSLAATSTWDYPVYLLFGMLAVGYEHFK
                     QQRYYPAIFPQCSQTVRIVISCALSLLIFAGSTYLFFLPYHNAYGAGYTSIQGWQGTH
                     TPLSSLIVHWGIFLFFIVSWLLIETRDWLSQTPISYLKTLRSYHPFLWVGGFIVVGTL
                     IWLLLKGVVIALVMLPLLLWIFLLLLSQKLSILDKIVLLLLLAGSAMILMVEVIVLTG
                     DVGRMNTVFKFYLQAWTFLGISAAYCFADLLPRLWKPAVEGWKRTWHACGIVLAVCGL
                     LFPVVASVDKITDRISDTVPLTLDGMAYMQTSVYWQDGAVMDLEQDYQAIRWMQENIQ
                     GSPVIVEGTSSLYQWGNRYSIYTGLPAVIGWDWHQRQQKAILPSQWITDRIAEVEAFY
                     NTTDGKTARNFLAKYQVRYILVGQLERIRYSEEGLKKFTTYEGIYWKTVFSYKDTIIL
                     EVIE"
     gene            25034..27553
                     /locus_tag="PLL88_03755"
     CDS             25034..27553
                     /locus_tag="PLL88_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HOJ00708.1"
                     /translation="MAVQIATWYLLMLGLGLLNYPVTSSLLPKLKSRGYAFSKIMGLL
                     LWGFLYWITNIFQVLPNSFMGAVVALALIASLNLSVFIRKKEELKRDFLEDKSYIVMV
                     ELLFFVAFVVSSILRAMSPALTGTEKPMELAFINAILETPKFPPSDPWLSGYAISYYY
                     FGYLMVALLARISGVGGGVAFNLGMAVWVGLICTAAYGILYDLIYAYFAHRKNKPQKG
                     KRKLLWLSLLAPFFLLAVSNAEGGLEMLHASGAFWGTDAQGNPQSNFWEWLDIQELNE
                     PPAVDFQWIPQRIGGTWWWRASRVISDYDAAGNFREVIDEIPAFTFFLADLHPHVLSI
                     PFFLLITAASLNLLMGGGMWLLHAQNWKDMLKTWEFWISALLFGSIFFLNTWDFPASF
                     GLFCLVFLLALIKANGWSWQTCLELTWKVVLLASGCVLMYLPFFLGFSSQAGGVLPSL
                     LYATHGVQFVVMFLPFIFLLSIYLIWINRGQKAPDKKIVPYFLLCIAGLCVLSLLFPF
                     SRQLSVFIWTRLQEWLGRFADQLNQAIQQARSFAAVYGALDLQSLMKETVTRRLRDSS
                     VLMMLSVFLYLAIRFFFHEPKIENEGRDEHHANEAFAPTFVVLLIVVGCGLCLIPEFF
                     FLVDVFSDRMNTIFKFYFQAWILFSLAGSFIIVVLWDAFRKWQKVVFRAASVFILIIC
                     CAYSFFIYRERIRYTRVENWTLNGSAYLAWSNPADAAVIEKLKTLPYGVVAEAVGGSY
                     SGYARIATISGYPTVLGWPGHEAQWRGGYEEIGSRQTDIKTLYETSDWTTAQYVLDQY
                     RIRYVVVGSLERTTYVVEEQKFAENLLLVLEKNGTNFYVVP"
     gene            27565..29268
                     /locus_tag="PLL88_03760"
     CDS             27565..29268
                     /locus_tag="PLL88_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00709.1"
                     /translation="MKQFNKVKLTHLFYALILLVAVFLRLVNLGTVPLTNNEAQHALC
                     VLDAQRDDCENYSRLYIFFTDIFFKIFGTNNGSARMLPALLGSTIVLIPLLLSSVVER
                     KTGILLAMCLALDPLLMQTSRSADDTMLGIVFVLFFLAFFLHRKVTAALITFAFGLLG
                     GRSFWIGLVILGLATGVTWLLTHREMRAELNAKIGKVRLGGKESRYLFLTLFLFWIGI
                     STRMFNDVGGLLSPLKSLLDIFPGAGTQRGTSAFSTDVRLIVFLFYSFYAISFSISAL
                     FHSNSDQKRRDIFLLVWILSGILIFLIPQFPFSQAAWVSIAMWVLAAGRMMQIGSSAW
                     RERKPLILPVLVGLTILVFLSFQLLRLQYLLSAGLDLSLNLTLLITPMLLCLLFILLY
                     AYGWSKPRAVQVLSILFIICGAVALLRNANRSSNLTGTHAYELVQEDSFFKNQDILIE
                     RIENYRNARGMPQGEVTIAICMDEPGERWEWVLRDYSVTVTDDISEETSNDYDMMIMR
                     ADSTQDFPRHESENLVLSGEVAWVRDDFSGFLPEEILEWLIYRTATLKTTSYAVWYKS
                     L"
     gene            29283..>29507
                     /locus_tag="PLL88_03765"
     CDS             29283..>29507
                     /locus_tag="PLL88_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOJ00710.1"
                     /translation="MELNNAITIDGPVASGKTTVGKQLAEKLGYQFLDTGIMYRAVTL
                     AALEDGIDLQNENAVSEKAEQIRIEILPPTK"
BASE COUNT         7476 a         6469 c         7610 g         7952 t
ORIGIN      
        1 aagccatcgt ttgtgatggc ttttcatgat gaagataata tccctaaaca cctgcaatgt
       61 aacagatagc gattacaccg ggtccggtgt gggttccaat gacggggctg aggctggtta
      121 tcaacaggtc ctgaatttct tgagggctat agagtttttt caactctgct tttacatact
      181 cggcatcttc ttcagcctca acctgcaaga ttgccagatg gacggggcgc tttgccccga
      241 tcatttcacg agcgatctcg atgatacggt taatggcttt cttttttgtg cgcacctttt
      301 cgtacgattc gatctttccg tccttaaaat agagaatcgg tttcagatcg agagcagaac
      361 cgagcagcgc agacgctcca ccgatgcgcc ctcccttgtg caggtactct agagtgttta
      421 ccataaaaaa tacattgctg ttggctactg ctttttctgc aagttctttg cattccttta
      481 atgaagcgtt ctcccttgca cgtcttgcag caagcactgc aatcaaccct aaaaccattc
      541 cggatgattt cgaatcgaca atttcaatat cttgcgagcc gagcatttct ttggcttgaa
      601 aagccgaatt gacagtgccg gaaattcctg aagaaatatg aatgctgatg atcttatcgc
      661 cagcgtccaa gatgggttta tacaaatcca tgaactcctt gggcgagggt tgagatgttg
      721 ttgggagatc ttttgcctgc ttcagcatcg tataaaaatt ttcgctcttc aaatcgacat
      781 tgtcctgata gaccttatcg ccccaaacga cgtggagagg gatgatccgc aggtccaact
      841 cttggatcag gttttctgga agatgtgctg tgctatcggt tactacaaca actttcgaca
      901 tagccgctcc tttcgaattg tcattaaaaa ttataacccc ttgtttttct ttcgttgctg
      961 taaattaatc catatgtaga tttcaaaaac cgtttataat atgcaagtta atgagttgag
     1021 gaatgacttt gaaagcgcat cctttcgcgc ccctcgcagt caactacctc taggaccgta
     1081 tcaataggag attgcaggag taaaacatgg taaaaattcg attacgcaga actggtctgc
     1141 gaaaccagtc aagttacaga gttgtggttt gtgacaagga agcgccccgc gatggtcgct
     1201 ttttagagat cgtagggttc tacaatccgc gtacagaacc attcactttt gatgttcaag
     1261 aaggtcgtgt ttactactgg ttgagcaaag gggcacaacc cagcgaatca gtggcgaagc
     1321 tgttcaagac tgttggattg accgaacgat ttgaaaaggt taaggctgga gaatcagagg
     1381 aaacagtttt gaaagaagca aatgccttct atgaaagtag gaaggcgaag aaataaccca
     1441 gatagagaag aagagcactt tctatcaaag gagtagtcgt gaaagaattg gttgaatata
     1501 tcgcgaaatc cttagtggaa gatcccactc aggtcagtgt aaatgaaact gaagagggcg
     1561 atcatgttcg cattgaacta accgtggcga aagaagacat gggtcgcatt atcggaaaag
     1621 gcggacgcgt ggctaattcc atccgtactt tactgcgtgt tgcggcggcg cgcgaaggga
     1681 aacgcgcgtc actggatgtc attgaaccgt aatggctgag agtccttcga attcgaagaa
     1741 cgagatcaac aatggctcgc ccgaaatcgg ggagccatta tttttaatta ttggaaagat
     1801 acgcaaaccg catggggtaa gaggggaaat gctggttaag ttgttgactg aatttcctga
     1861 acggatcgag gaaggaaaac gggtatacat tggggaatcc aaaacggagc acacaatcgc
     1921 gacagtgcgc tttctgcatg aaggctgtct gataaaattt tcggcggtca actctccaga
     1981 acaagccaga caattctgca acgagatgat ctatgtgcgg gctgttaact tgccaaaact
     2041 gcctgaaaac caatattatt atcatgatct gcttggtatg caggtaaaaa cggacgaagg
     2101 ggaaatgctg ggaacggtac atgagattct tgaaaccggc gcaaatgatg tgctggttgt
     2161 gcgaaaagga gaacaagagc acttatttcc ctttatcaag caagttgttt tggatgtaaa
     2221 tcagcgagaa aactatattt tgatcaagaa actcgaatgg aaatgacatg gatgaaaaga
     2281 aatactgggt gggatttaac catgtaaggg gcatcggcgc agtacgattt cagcgcttgc
     2341 tggaatgttt tggaaatctt gagaaagcct ggaacgctcc cgaaattgaa ttaaagaggg
     2401 cggggatcgg cgataaatct ctggtgaatc ttgttcgttt tcggcgagaa catgatctcg
     2461 atcacatcta tgaaaatatt ctcaagaaag atatcaagat cctgacctgc atggacgatg
     2521 attaccccca gcaattaaaa cagatcaaca atccaccacc ggtattatac ataaaaggag
     2581 aaatggtaag ggaagatgcc cattcagtgg cggtggtggg taccaggcag ataaccgctt
     2641 acgggaagag ccttgttcag gaattgggaa gagtgcttgc ccaaaatcac atcacagtga
     2701 tcagtggctt ggcacgcggg gtggatgctg aagcccatcg aacagcgctg gaagcgaatg
     2761 ggcggaccat tgcagtgctg ggctgcggca tcgatatcat ttatccgcct gaaaaccgtg
     2821 ccctagccag tcagatcact gaacatggtg cgctaattag cgattatgcc ccgggcacac
     2881 aacccgaagg gatcaacttt ccgccgcgga atcgcatcat tgccgggtta tcgctggcta
     2941 cgatcgtggt ggaggctggc gcgaggagcg gcgcgttgat aaccgcttca tttgctgctg
     3001 accagggcaa agatgtcttc gctgttccgg gatcaatcta tgctccccag agcaaaggta
     3061 ccaacaaact gatctttgat ggagcgtacc ccctcatccg gtttgacagc atctttgaaa
     3121 ttctaggtct ggacaatgta caatatcaga agaaaacaca aagcgagctt cctaccgatg
     3181 aggcacaatt attggtgttg aagatattac aaaaagaagc actgcacatc gatgatatcc
     3241 aggcgatttc ccaactgcca attgagcggg tctcatcagc gttgagtttt ctggaactgg
     3301 aaggattcgt acagagaacc ggaaatatga ctttttctgc gatttatgat ttcaaagagg
     3361 agtattcttg aaatgcatga taattatttt gccgttatta tggctggtgg cggtgggacg
     3421 cgcctgtggc cactttctcg ccagttgaaa cccaagcaat tgttgaaacc gtttggcgaa
     3481 cgaaccttct tccagatggc ggtagataga ctttctcccg ttttcacgcc gaaccatatt
     3541 ttggtggtca cggtggcttc ccatgtagct gagttgcgcc agcaggttga ctctatcccg
     3601 ttggaaaatt atctgattga acctatgcca cggggaacgg cttcagtggt gggttatgcg
     3661 gcagaagtgc tgcagcaaac atatcctgat gcggtaatgg cggtgctgac atctgaccat
     3721 atcattgaga atgtgcccct gttccatacg ctcctggaaa acgcgtatga agtagcgcaa
     3781 aatggctttc tggttacctt gggcatccaa cccacttttc cctcaacagg gtatggatat
     3841 atccataaag gtgaaaaatt atcagctttt cccttctccg cgcatttggt tcagtcgttt
     3901 gttgaaaaac cagatctgga atgtgcaaaa caatatctgg cagccggatc gtactattgg
     3961 aattccggaa tgttcgtctg gaatacgaaa aacattctgg atgagttcaa ccgccaaatg
     4021 ccggttctat attccagttt ggaagtcctg gggcagcggt atggaaaaga agggtacgaa
     4081 acattgctgg cagagacctg ggaaaaaatc actcctcaaa caattgacta tggtattatg
     4141 gagcacgccc ggcaggtggc ggttatacct gcggaaaatc ttggatggag tgatgtgggt
     4201 agctgggatt ctctatttga gatcatccag gcggatgaca atgggaatat caatctcgca
     4261 gactcttcca tcatccagga gagcagcaat tgcttgatca aaactgataa tgataagaag
     4321 atgattgttc tggcaggact cgacgatatc atcatcgtag atacgagaga tgcacttttg
     4381 atttgccgaa aaggacagtc gcagagagta cgggagatcg tgaacgaatt aaaagaaaag
     4441 aaactagacc agtttttata ggagcaacca cgtggaagcg tattgcgtaa agtgtaaaga
     4501 aaaaagagag ttacagaatc ctgtagcagg tttcaacaag aggggagcgc ctatcacaac
     4561 cggcacttgc gcggtctgcg ggacaaaact ggtccgcatt ggaaagaccg aagcacatgt
     4621 cgggctggaa ccacctaaaa atgagaagaa agaagtcaga tcagaaaaac tggtgattgt
     4681 cgaatctccc gccaaagcca agaccatcgg aaactatctc ggaaaggaat ataaagtcaa
     4741 agcgtcggtc ggtcatattc gtgacctgct gcgttctcag ctatcggtgg atgttgagaa
     4801 taattttgcg cccaagtacc gcgttccgaa tgagaaacgt cccattgtga aagaattgac
     4861 gaaagatgtg caaagtgccg gagagatcta cattgcaact gatcccgacc gtgaagggga
     4921 ggcgattgcc tggcatttaa aagaatctac caacatggat gaatcgcggg tccaccgcgt
     4981 tgtgtttcac gaaatcacca aaccggctat tcaggaatcc tttgcccatc cgaaaaatat
     5041 cgacatggac ctggtaaatg cgcaacaagc ccggcgcgtg ttggacagac tggtgggtta
     5101 caacctgacc cctttattat ggcagaaggt gcgtggacgg ctgtcagcag gcagggtgca
     5161 atcgatcgca acccgcttga tcgtagagcg cgaacgtgag atcgatgctt ttgttcccca
     5221 agaatactgg tcggtcgaag ttgaattcaa accagagaac ctgaaaaaga cctacctggc
     5281 tcagttaagc ctgatcgatg gaaaagaatt gactttgtcc agtcaagctg ccgtggatga
     5341 aattctagag gatattaaaa aagccagtta cgttattgga aaaatcaaaa aaggagaacg
     5401 gaaacgcagc ccctccgccc ctttcatcac cagcaccctg cagcaacagg cggctcgcca
     5461 attgaatttt agggccaggc gaaccatgat gatcgcccag caattatatg aaggtatccc
     5521 cctggatggt ggaagccaaa cggggttgat cacctacatg agaactgact cgaccaatat
     5581 ttctccaatc gctctgaatg aagcgcgcca atatattcaa tcggtatttg gtgattcatt
     5641 cctgccctcc agtccacgtc tttataagac aagagctgcg gttgcgcagg aagcgcatga
     5701 agctatcaga cccacctctg tatttcggac gccggaaaag gtgaaagatt ttttgagccg
     5761 cgaccaatat agattgtacc aactgatctg gaaacgattt gtggcttccc aaatggaaaa
     5821 tgcgatctac gataccatct cagtacaggt ggatgggaat ggcgataaac accagtatgt
     5881 attgcgtgca tccggctcta aggtgcgctt tcctggcttt ttgacccttt atgaggatgc
     5941 cagggaagaa gaccaaaagg atggtgaaaa gaatataccg atccctgatg gaatcttcga
     6001 agggcaaaaa cagattctga tcaacatcaa cccgcagcag catttcaccc aaccccctcc
     6061 gcgctattcc gaagccactt tggtccaggc gctcgaaaag aatggcatcg ggagaccgtc
     6121 tacctatgct cccatcattt ctacgataca aacccgcggg tatgtggaac gcgaaggtag
     6181 caagttgaaa ccaaccgaga ttgcctatac ggtgaatgat ctgttggtgg aatattttcc
     6241 agacgttctg gagatcaatt tcacggcaga gatggagcaa gacctggacg aaatcgccaa
     6301 tggagcgaaa gattgggtgg atgttttgaa aactttctac ggggcttttg aaccgcgtct
     6361 ggaacatgcc cgcgcagata tgccggaaat gaatgccgaa ccagagaagg tgggaaaagc
     6421 gtgcccacgc tgcgggcacg atctcatcat tcgctggggt cgctatggaa agtttatcag
     6481 ttgtagtaat ttccctgaat gccgctacac cgaagcctat cttgaaaaaa tcggtgtgac
     6541 ctgtccgaaa gatggaggcg agatcgtact gcggaaaacg cgcaaagggc gaatcttcta
     6601 tggatgcgag cactatccta attgcgactt tacctcttgg aagagacccg tttccacacc
     6661 atgcccagcc tgtaagggat tgctgaccat aaaaaataac aaagaattgg tttgcgcaga
     6721 ttgcggaaat actttctcga cggatattct tgaaagtcaa gaagaaatgg cataaaactt
     6781 gctatttaag tggtgaatta cagggattca tagaatccgt tgggagaaga aaccatgaag
     6841 aatgtgcgaa aaacttacga gaaacctttt ttctggcctg ttgtaacgct tatcctgggc
     6901 cttgttttgg gattggtggt acttggatgg tggctctggc cagtggagtg ggtggatgca
     6961 tccccgtcac aaatgcaaaa tgagtataaa cgcgattatc tttgtatggt cattgattct
     7021 tacgtgcgca atcatgacga ggacctggtg aacaaacgat tgtacgcctt gggagaggat
     7081 gcggaggcat atctcaatat gctcacgcct gaactctgca aattcacatc ggctgaagag
     7141 atcaccagtt ttcgtgcctt aaaattcacg gactcaacgg taacttcgaa cacgataacc
     7201 gaacaagcgg ctgaattgac caaggaacag gcggatggtt ccaagaagac ctctctgggg
     7261 aacatcttgt tgatcgccgc actggttctt ttggttggag gcggagcttt ctatatcctt
     7321 gtcaaacggg gaaagattaa tctgaacagg caagtcgata agcgcagaga agtgaacaag
     7381 ggatttgaag tggcttctcc tgttgtagag gaaaccgctc aggcaagcca ggaaccccca
     7441 ttggctcaat tcatgaccac ataccggctg ggagatgacc tgtatgacga ttctttcagc
     7501 atcgattcgc cttcaggtga atttttgggt gaatgcggag tgggcattgc agagacgatc
     7561 ggcgtgggag acccgaagaa agtcagcgca ttggaggtat ggctgttcga caagaacgat
     7621 attcaaacta tcaccaaggt tttgatgagt gcccatgtgt tcaatgacac agcagccaga
     7681 caacgattgg cagccaaggg agagactatt ctggttgaac cagggcaacg ttttatcatg
     7741 gaaacggcta ccttgcaatt ggaagcgcgc gtggtagata tggtctacgc acgggatgca
     7801 ctccctgaaa acagccattt ctcgcgatta acgttggaaa ttgcagtttg gagcaagatg
     7861 taaaaaaact gcccttcggg gcagttttta tttgatatcg aggatctgga aaagcagata
     7921 ccctgcgggg gtggacactt taaccgtatc tccaaccttg tgtttcagca acgcgctccc
     7981 aatgggagat tcaaaggaga tcttgccatg ggcaggatcg gcttcggctg gacccaccaa
     8041 aaaataagtt tcagggctgt cagagctctc ctggatggtg actgtggtcc cgatctccac
     8101 cgtatcgtgc acattctgtt ttccatcaat gacgatgtaa tttttcagca tggcttcaat
     8161 ctcctggatg cgaccttccg tgaaagcttg atcttccttt gctttgctgt aatccgcatt
     8221 ttcagagagg tcaccctgct cgatggctgc ttttaaccgt tttgccagag caactcgagc
     8281 cgggcctttc aattcagcta attcctgttc cagtttttga acgccttctg gggttaaata
     8341 aacggtatct gccatgttgt gcttacctcg attgagcaaa cattttacta taaaacgatc
     8401 ggttttactg atgatccttt aagacggttc cgacctctgc gcccctgaga gcctgatata
     8461 aattcccttc ctcttttcca ctgaatatgg atgcctggat ggcgggattc cggttgatga
     8521 tcgaaaataa gacctcaact ttcgagcgca ttccccccgt cacatctggt gatcgcgaac
     8581 cgctaattcc tgcattctgt gcatggaaga gcgcaggggt aaaaacgggg tagatattgg
     8641 ctggggaatc tgcctgtttc caaattccct gttcgattcc aacgatcagt atcctttgta
     8701 ttggaaggtg caggcataaa aatgcaagaa tttcctcggt agataggatg gttccgccta
     8761 tctccaaatc aaacacaaca tctccaaaaa ctaccggcaa aagggaatga tcgagggcat
     8821 tgtgcaaagg cgcagtatcc cactgcacaa cggcatgatt tttggtaatg gctacggcag
     8881 aaagcggaaa gctaatggcg ttcagcccga tctgccataa ttcgtccatc acgatgcggt
     8941 gtagtgcatc tgcctgtcga cgcacttcaa taaaaccgca ccattcctcc gctgaatgaa
     9001 ctccgctgcg ggtctggtat ttcttagcgg gtggatgtcc gaaagagcca gagccatgcc
     9061 caagaatgat ctgtagcgca gggtcgtcct gcaaagcctg gcttacttcc tgtgccaggc
     9121 gagaaattac cacacctctg gctgaacacg cctggttctt atcggtaatg agcgagccac
     9181 ctagtttaac aaagacaagc atttctattg aatgaccgtg tgaacgatcc ctttaactcc
     9241 atgtgatacg agcgcttgct gaatgaaggg tgcgttctct tcaccaacta atgctatcac
     9301 atttcctccg cgaccagctc cgcataattt tgccccccac gcgccattgt tcaaagcgag
     9361 cgaaaccagc gtatccaatt cgggcagcga tacgtgcaat gcctgcaaca gagcgtgatt
     9421 cctctgcatg agtccgccga gctcctgggc gttgccgcgc aggaaagcct tttttgctga
     9481 ttgcgtgatc tctccgatct cttgaaaaat agaatttatt gtctgtggat cattttcgcg
     9541 ggcttgcttg accgcgctga ccacttcaac agtatgggat gcgatgccgc tgtcgatcaa
     9601 caaaatgtga aaaggggttg gtgactgtag aaattcgggt tcgagaccct tggtgaaaca
     9661 tagcgctttt tcataactga taactgtgtt atcgattccc gaagggttgc catggtgtag
     9721 cttttccagt tcaaaggcaa aatcattgat cttttgaact ggcaatttca atcccaaaaa
     9781 ctgcgaaagc gctttcacca cgccaaccga aaccgaagcg ctggagccca atccggaagc
     9841 aacaggaatg tgggatttta ttttcaagcg aaaggatgga atggtggaaa tggcatatgc
     9901 gtccttgaac agcttaaccg cgtgaaccag aacattgttt tcaggcaggt cagagagttt
     9961 ggcggaaata gctagatcgg gggcatcata gatgctcacc tgctgctctt tgccgatcaa
    10021 agggaaaatg gtaacagtgg ttttgagggc tggaatggga acagctattg cagggtaacc
    10081 ataaaccacc gcatgttctc cgaaaagaat ggactttcct accgtatgga cgacaatcgc
    10141 aggcatttta tctaaaacag ggctatgatc ttagcccaat agaaaatgac gatcacgctg
    10201 agcccccata gcaagataca tacttgcaag ggtctatcag aaagaaccag atcttcaggt
    10261 gctccacccg atttgcggac cttgatcaaa tataaatacc gaaacaagcc atacaagaca
    10321 aaagggatgg taagcatcat cgtgttgttc tcaggcaggt taggggcgga gaaagtgtac
    10381 aaactgtatg ccataatcga agtgcccgac acaatggtga tcaattgatc gagaaattcc
    10441 agagaataac catccaatac tttgcgatgc tgggttgcgt tgtcgctgag gcaaatgatc
    10501 tccgcacggc gttttccgag cccgatgaat aaagccagca gggatgtgca gatgtataac
    10561 catggagaaa aacgctccac ttggatgaga gagaccccgg cagccacccg caggacaaac
    10621 ccggcggcga tgaccatcac atcgatcaga gggatatgct tgagtttgaa tgaataagcc
    10681 aggttcaaga caaagtaaac agcgctgatt accccaaaac tggtcgatag acgaaatgcc
    10741 ccgtaaagag acccaaccac tacaagaact gccacaggaa tggcgacgtt ggtgggtaat
    10801 ctacctgaag cgatgggacg ctcttttttt gtgggatggc tttgatccgc tttcttatcg
    10861 aagacatcat tgatgagata taccgcactg gaaagcatac agaaaatgaa tacgccgata
    10921 agtgtggtca gtgtggctga ccaattggta agctggcgat cgaatacgag cgctgccagg
    10981 atgaatacgt tttttaccca ttgttttggg cgcatgcttt gaatgatggc ttttaacata
    11041 tgcgaatcat aacatgaaaa aatcgtggaa gtcatcagga tgtaaaatga agctatgcag
    11101 aagaaaagac tggacgtgtt gcttgttgaa aaagggctgg ctgaaagccg aagtcgagcc
    11161 caacggttga tcatggcggg ggaggtcatg gtaaatggtg ttcagcgatt aagaccttcg
    11221 gaaatctatt ctgacgaaat agcgatcgct ctgagggaaa ccgctccatt tatctcgcgc
    11281 ggaggagaaa agctagaagc atccattcaa gcgtttggtt tttcggacct cactggcaag
    11341 gtctgtgcgg atatcggagc ctctacaggt gggtttaccg attgtttgct gcaacatggg
    11401 gcagaaaagg tgtatgcggt agatgtgggg tatgggcaat tgcattggaa gttgcgcaat
    11461 gatccacggg tggttgtgat ggaaaaaacc aatgtgcgca atctgaaaac gctccctgaa
    11521 aagatggata tggtggttgt ggatgtttca ttcatatcct tgcgcgcgat ctttcccgtt
    11581 ctgaagggac ttatgaaaga atcagaggca gcgatgattg cgttgatcaa gcctcagttt
    11641 gaagcaggaa aacaggaggc agcaagaggg aaaggggtga taagggaccc tgtggtacat
    11701 aaaaaagtgc ttgagaacgt tctgcaggct gcccaagcgc aaggcttgta cctccacggg
    11761 ctgatcccat cgcctatcaa gggtccgaaa ggaaatatcg agtttttggc tctattccgg
    11821 gcagagccag gacaagagaa tctcgaacaa cgtattcaac aggttttgga gaaagaggtc
    11881 ggcaccgctg agccgcagga aacccaataa ggaaaatcga ttatactaat cagttgccat
    11941 gcaattaaca caaaaaagaa atttctgcat cattgcccat gttgatcatg ggaaatcgac
    12001 cctggctgat cgtctcttgc agatgacggg ggtcatttca gaacgtgata tggtcaacca
    12061 ggttctggat aatatggacc tcgaacggga aaaaggggta accatcaaag cttcagcggt
    12121 tcgcatggag tacaaaaaaa atgggttgat ctacgaactg aatttaatcg acacccccgg
    12181 tcatgtcgat ttctcttatg aagtaagccg ttccttgatt gcttgtgaag gtgccgttct
    12241 ggttgtggat gctacccaag ggatagaagc acaaacctta gccaacttat acctggcgat
    12301 cgaatctgat ctggtcatta tcccggtgtt aaacaaaatc gacctgcctt ctgcacatgc
    12361 ggacgaagtg gcggaagatg tgggtaatct gttaggttgt gatcctgacg ccatcattcg
    12421 catctcggca aaaacgggtt tgcacgttga tcaggtgctg gaagcgatca ttgaacgtat
    12481 ccctccaccc aagggagatg agcatggagc gctcaaggcg cttatttttg actctcatta
    12541 tgattcttat aaaggcgtga tcgcttatgt gaggatattc aacgggaaag cggacatgag
    12601 tggtaagttc ctgctgatgt ccaatcatgt cgaaacggac cctattgaga taggaatctt
    12661 tgcgcccacg atgaaaccgg tagacgtgct gactgccggc gatgtggggt atatcgccac
    12721 gggtttaaaa acggtaaagg attgccgggt gggcgatacg cttacagata aaaataatcc
    12781 agcagaacac agcttgcctg gttataaaca tcccaaacct atggtctttg ctggagtata
    12841 tccagtggaa ggggaggact acgataattt aaaggatgcc ctggcaaaat tacagctcaa
    12901 tgatgcctcc ttggtcttca cccccgaaac ctctcaggct ttgaactttg gttttcgttg
    12961 cggttttctt ggactcttcc atatggagat tttccaggag cgtctggagc gtgaatatga
    13021 tctggatatc gtgctgaccg cacctaccgt agagtataag gtcattctgc acagcggaga
    13081 agaggttctg atcgattccc ccgccatgtt gcccgatgag tcaaccatcc aggagattca
    13141 ggagccctgg atgaaccttt ctattttcac acccaccgaa ttctatggcg ccgtgatgga
    13201 actggtgaaa cggagattgg gagtgttcag ctctcaagaa tatccatccc ccaacagggt
    13261 tcaattaagc ttcgagatcc cattgtcaga aatgatcgtt gatttctttg acgagttaaa
    13321 atccaagact cgcggttatg cttctatgga ttactcgttt aaagaatacc gggctagcaa
    13381 tctggtcaaa ttggaagtgt tgatcggtgg agagccggtg gatgccctgg ctagtattgt
    13441 gcacaaagat ttggcttatc atcttggtca gcgattggtc acaaaattga aagaactgat
    13501 tccacggcaa ttgtttgacg tcgctgtgca agcctcagca gatgggcgca tcatctcccg
    13561 cgcaagcatt aaagctatgc ggaaagatgt gctggctaag tgttatggcg gtgatgtgac
    13621 cagaaagaaa aaactgctgg aaaagcagaa aaaagggaag cgacgcatga aaatggtggg
    13681 aaatgtggag atcccccagg aagcttttat ggctatcttg aaattggagg atgaataagt
    13741 tgcaatctca agaaaacatg aaaaattcat ggaaacgatg gctgccgggc gtggtggtca
    13801 gtgcgattgt gatttatgtg ttgtaccgca taatcgatat tcccattttt ctttcagcga
    13861 tacggaagac gaatttattc tatggaatgg tcgcgttgtg cctgatggtg ctggcaaata
    13921 tctcgcgcgc agcagcatgg cgagaattgc tgggaagaaa gatcagctta agggacgcat
    13981 ttcatatagt caatgaggga tatttgctca atcagatcat tccacgttcg ggagaggttg
    14041 gtcgggcgtt attggtgaac agcgtggtgg agatgaattt ttttgaagtt ttctctacca
    14101 tcatcatcga acgggctttc gatctgggta tcactgcggt gatgttccta tcgaccattg
    14161 ggaaggctat cgctatggat tggattgttc cggtagcgat cactattctg ggcatcgtat
    14221 tagccgggtt tcttttcctt ttctgggcta tcaagaaaaa aactgcggtt gaaagctggt
    14281 gcgatcgcat ggatcaaaaa tctgtatttt tccggaaata tgtttcccct aacataaaag
    14341 ccattatccg cggcgcacag atcatgcaag accctagccg tttattgctt gcgtttctgc
    14401 tgatcgcagt ttgctggctc ttgtggatgt ccatttcatt cattctactg agcagctttg
    14461 tgggcgtcaa accgttctgg tgggtagtct ttatccagtc cgtcctttcg ctggggatcg
    14521 cgctaccatc tgcgccagcc ggtctgggtg tctatgaagg aaccctggtg gcagctctgg
    14581 cagcatttgc tatcgaaaaa gagacagcgt tgggttttgc aatcatcatg catgctgtcc
    14641 aactggtggc gatcgcggtg ttaggaatct acagtcttgc gaaacagggc aactctctgg
    14701 gtcttctgct aaacaaagtg acgaaccgct tgcggaaagt aaaggagtga gaatgtctta
    14761 tcaatacctc atcactggag gggcgggatt cattggatcg aactatgctc accgacttct
    14821 tttaaaggga gaagatgtga ccatttttga taatctatcc agaaacggca ctgaaaggaa
    14881 cctggaatgg ctgtatcagc agcatggcaa aaaagcattc actttcattc aagatgatat
    14941 tcgcaatgct actgcgatcg aaaaagctgc ggatggaaag caggtgatca ttcacctggc
    15001 agcgcaggta gcggtcacca catctgtggc agagccgcgc gaagattttg aggtcaacgc
    15061 acagggcacc ttcaacgttt tggaggcagc ccgcaaatcc gggaaccagc caattctcat
    15121 ctatgcgtca accaataagg tatatggagg catggaagag gtaaaggtca aagaagaacc
    15181 tacccgttat tcttatgttg attatccaca cgggatcagt gaggattatc ccctggattt
    15241 ccattcgccg tatggctgtt cgaagggctg cggggaccag tacgtccgtg attatcagcg
    15301 catgtatggc atccgcacgg tggtattccg ccagagttgc atttatggta accgccaatt
    15361 cggtatcgaa gatcaaggtt ggttagcctg gttcgtgatt gcagccgcgc aaggtaaacc
    15421 tatcacgatt tatggggatg gaaaacaggt gcgggacgtg ctttttgtag atgacctggt
    15481 ggacgcctat gatagtgcca ttcaacaaat cgagaaggca tccgggcagg tctacaatgt
    15541 gggtggaggc ccagccaata ccatttcggt gtggcaggag tttggagcat tgttggaaaa
    15601 gaagcttgcc aggaaagttc ccgtgagcac ctcgaattgg cgcccgggcg atcaaaagat
    15661 ttacatttct gatattcgca aagccgggca gcaactcggc tggcaaccgc gcatcgccgt
    15721 tgaagaaggc atcgcccgcc tggtagactg gatagcaagc atcgaaactg aatgaccggc
    15781 atgaagatcc tgatcgaact gacctattat cgtccccaca ccagtggttt gaccatctat
    15841 gcggaacggc tggcgaaagc acttgccaaa cgaggtcacc aggtaactgt gttgacctct
    15901 caattcgata aaaaattacc gcgtgatgaa tggttggagg gagttcatgt tgtccgcttg
    15961 ccagtgctgt tcaggatcag caagggggtc gtcatgcctt ccttggggtt catggcgacc
    16021 aagctggtca aggaaaatga cgttatccat ctccatttac cccagattga tgccgcaggt
    16081 attgcattga ggggaaaatt atggaaaaaa ccgacagttt tgacctatca ctgtgacctg
    16141 cgcatgccgt ggggcgttat gagctggctg gcaaaccagg gagtgcatat gatgaataac
    16201 ctggcagcca tgttcaccga tcgcattgtc acctacacgc gcgattatgc agaccattcc
    16261 ccttacctga agcgtttcca aaagaaattg cacattattc ctcccccggt agaattgccg
    16321 gttgctgcga agcaagcgat tcaggatttt gccaggcagc ataacccgct ggggaaacgt
    16381 cctatcattg gaatggctgc gcgttttgcc tcggaaaaag gggtggaggt tctgctgcat
    16441 gctatgatga gcgtgcgaga agtttttccg caagcagaag tgtggtttgc cggtccgtat
    16501 gagaatatca tcggtgaaga agcgtatttc aagaggttag aacctgttat tgaaaaaatg
    16561 atccgggaag gcgcatggaa attctttggt tcgcttgccc cggcgcaaat gtcgcttttc
    16621 tatcccaaca tcgatatgct ggtcatcccc agcctaaatt ccacagaagc gtttggtttg
    16681 gtgcagattg aagcgatgat gaatggaaag cccagcatcg cttccaatct gccgggagtg
    16741 cgccagccag tacagcggca tgctatgggg aaggttgttg aaattggcaa ttcaaaagaa
    16801 ttggctgaag cgatcattgc tgttgctgag aatcccgggc agttccatgg cgatggaaag
    16861 caaatcaaga cgatctatgc tcccgatgaa attgcccggc agtatgaaga cctctttttc
    16921 gggatcatgg ctgagatcaa acatggacga aaaacgaaac gctcttgaga aaagagattt
    16981 tctatgggag aacctgaagc ggctacccta ttttagagcg ctgttgcgtg cggtggaaag
    17041 ccgtttttat gaaaacattc cattgaacga accggttctg gatgtgggct gcggcgatgg
    17101 catctttgca gaaactacct tcgaagaaaa gatcacggtt ggcttcgatc cggcgctgac
    17161 cagcctgcga gaaccggcta accgaaaaac atattccagc atcctgtgtg cagcgggagc
    17221 agcgatgcct ttccagagtg cgttcttttc taccgttatc agcaactctg tcctggaaca
    17281 cattcccgat ctggatgcag tggtagcaga gatccaacgg attttaaaac caggcggatt
    17341 gttcgttttt tgcgttccca atgaccaact cttgaaaaac ctgtcgatca gcaagtggtt
    17401 agacaggatg gggttgcgtg gactggcgaa ggcgtatcgt acatttttca ataccatctc
    17461 tcgccaccac cacgcagaat cattcgaggt atggcagacc cgcttagaaa acaacgggtt
    17521 caagattgaa aaatggtggc attacttttt tccaaagcag gtgcatgcac tggaatgggg
    17581 ccattacttt ggattgcctt ctctggtaaa caaaaaaata ttcggaaagt ggattctctt
    17641 gccttttcat ggaaatccat tcattccaga aaaaagattg cgaaaacttt acaaccagga
    17701 tcctgttcat cttgaaggca gttatacttt ctatatagtc agaaaggatt gaatgcctga
    17761 gaaagagcca agtctattag attatttgcg atcactgctt gaattccaac ctgacaggta
    17821 tcgccattat ctgcgtatgg atgaaacggc agctcacgtg ccaacgaaag cacaatcgaa
    17881 aaagggtttt ccatacaggt tcgcttttct tcctgtattg gcattggtgt gcgctctggg
    17941 gggacagttc ctgttggaac cggcacatag aataaccccc gcgaacgtca ttttttatgg
    18001 gatggcggta gtgctggtta tcgtttttat tctttcgaat cgtgcccatt ttcaggaaaa
    18061 ggtagatcgg gaaaaagcgt ggtttgtttc gcaagaaatc cgggtgaatg tattatttcc
    18121 agccacactg ttcctggctg ctgcgttctg gtttttccag gacaaccaat ttaccgttct
    18181 caatgtagtg ttgtggatcg ccggattgct gctctttgtt ctatctgtgt gggagaggaa
    18241 agaaattcgc gctcgcagca agaagggatc aggcttgttc tggattctca tgatcctggt
    18301 ggcaggggtg agcatttttt ttcgggtgta tgacctgcag gaagtgccct cagaaatgtt
    18361 cagcgatcac gcggaaaaat tactggatgt tgcggatgtt ttggatggaa acactcccat
    18421 tttctttatt cgaaacaccg gtagagaagc gttgcaattc tacctgaccg cgctgatcat
    18481 tcgagttttc aacacaggca tttctttcct gagtctcaaa ataggcacgc tgctggcggg
    18541 aatcctcaca ctgcctttta tctatctgat cggaaaagaa ttgggcggaa aatggagcgg
    18601 tctggctgcc ctttttctga caggggtcgg ttactggcca aatgtgatct cgcgcgtggc
    18661 gctgcgctat gccttctatc cgctattcac cgccgcgaca ttctattatc tgatcagggg
    18721 actgcgcacc cggaatcgca atgattttgt attgacagga cttctgttgg gattgggatt
    18781 acacggttac agttctgccc gttttctacc gattgtggtc actgcgattt ttcttctcta
    18841 ttttcttcga ttgagaacca ggcaggaaaa acataatctc atctggatgt tcgttctggt
    18901 tgccctgact gctttctttg ttttcttacc gttgtttcga tacgctttgg aaaacccgct
    18961 catgtttggt taccgggctt ctacgcggtt attccctgtc gaacagggct atcctgacaa
    19021 tccgctgctc atttttctaa gcaatttttg gaacgcgctc accatgtttt atgccaataa
    19081 cggtgtgatc tgggtgcact ccatacccaa ccgcccggcg tttgacgtgg tcagtgcggc
    19141 tttgtatttc ctcggcactc tttttgccgt aattcaatcc ataaaaagaa aaaattggct
    19201 gaccactgcc atcctggttt ccatcccttt gctgctcatg ccttctatcc tttcgctggt
    19261 atttccaagc gaaaatccca gcctgaatag aacaggaggg gcatatgtgt tggcttttgt
    19321 ggtagcgggt tacggctggg cgcaggtcgt agaaatcttc ttgggttctg ctcccacaaa
    19381 agctcgcaaa ggcgttgcct tgttgttgac cctggttttg gcatgttatt gcgggatgca
    19441 gaattatgac ctggtgttca accaatatcg cacgcagtat gatctgaatg cctggaacac
    19501 aacgcagatt gcagaggaga tagggcgttt ttatcgcgag ggaggggaag tggacgacgc
    19561 ttttgtggtg ccttatccgc attgggtgga tacccgcttg gttgggatca acgccggttt
    19621 accgcgcaag gattacgctt tgtggtcgca agattttgtg accgttcaac aggaacccgg
    19681 aatgtatctt ttcattttga agccagaaga tgtggaaaac attgctttgc tgcaggattt
    19741 gtttcctgac aacatactgt atacgtatgt ttccgatact cccggcaaaa atttcattga
    19801 gatgcgagtg caaaactagt tacaatgtag aaattctatg aagaagacac tccagtcaaa
    19861 acgttttcac ataacattgc ttttgttgat tttgttcatg agcggattct tgcgcttgcg
    19921 ggggatccaa tgggatggag atttccatct ccacccggat gaacgttttt taacattggt
    19981 ggaaacaagc atttaccccg tcgattcact aagagaatat tttgacacca gcgtttccac
    20041 gttgaatcca cacaatgtag gaaaaacctt cttcgtctat ggaacttttc cgatttttgt
    20101 ggtgcgttat gtggcggagt ggcttcagca aacgggatat tcccaggtgc atattgtcgg
    20161 caggtacctt tccgcattgt ttgatctggg cgtggtcttg ctggtctatt gcatcgcaaa
    20221 aaagctgttc aagagaccgt ttgtcagttt attgagcgct cttttttatg gtctggcaac
    20281 cctgccgatt cagcaatcgc attactttac tgtagatacc ttcgcaaatt ttttcgtggc
    20341 tgtagcgctg tatctggcga tctgtatatt tgctctaaaa gaagaggaaa ttcagaatga
    20401 aaagaaatat gcatcgcatg atcgccggat gcatgtgatt ttcgggttca tggtcgggct
    20461 ggcagccgcc tcgaaaatta atgcagtggt gtgcgcagtt ttgctgccct tggcggatat
    20521 cctgcgtgat tatccggaac tgaagcaatc gccgcagagg cgcaaccaaa cgctgataaa
    20581 tatcgctctc gcagcattcg tttgcctggt cacctttcgc atttttcaac cctacgcgtt
    20641 tcagggaccg ggcttctttg gaatttccct caatgcgaaa tggttggata atttgcgcga
    20701 attatctgtt ttaagttcgg gagatatcaa ttatccgcct tccttacaat gggcgcgcag
    20761 accgtcttat tttgcctggc agaacctgct gcagtggggt ttcggtttgc cgatcggaat
    20821 cccagccttg atcggattgc tcgtgatggg gtggaagatc gtcagggaaa attggaaacc
    20881 gtatcttttg ttgtggatat ggatcgtact gtacggcgga tggcagagtc aggtctggaa
    20941 tccaaccatg cggtatatgt tgtatgtcta tccggcgctg gctatctgcg ctgcctggtc
    21001 ggttgtgagc gcggtcaatt ggctgttcca agagaaaaaa gtattcagaa ttgttctggc
    21061 agtatgcctg ttggcggcac tgctgggtgg aagtgttgcc tgggcttttg gtttttcggc
    21121 gctctatacc cgcccggtta cccgcgtggc agccacggaa tggatctaca agaacgtaga
    21181 aggtccgatc aacttgcaga tctccggtgc ggatgaagac ttcagtcagc cattggcata
    21241 tcctcattat attgatctca cgccggcgaa tcccatcacg tttaaattca ccccagaaca
    21301 agatgaaacg ctttctgcag taatgattga taagatccaa aattattcgg aggaatctga
    21361 cgatcaggcg cttacggtga gtttgtatga gatggcaaat ggcacccctg tgcttttaca
    21421 gagcgcgctt gtatcattgg tggattggaa tgagaataat gtgatcaatt ctcagtcggt
    21481 gcaattttcc tcttctccgg cgctgcagca tgataaagaa tatctggtaa aaatagaact
    21541 ttcagatcaa tctaccgaac tgcgcttatc cgggtttatt aaaaccgctg catatgatga
    21601 aagttcgatc cacttcacac aaacgatctt tgaatgtgct ccctcccttt cgcagatggg
    21661 catatatacg gtgcagttca ctccttttga ggacggggcg ttgaccggca taactttctt
    21721 ccgcgttgtg gattgggagg gaacagaaaa tgagaaggaa attcgtgtac gcattcaatc
    21781 tatggatggg gatgagaatt cttttgtaac cgcaacattg aagtccgcgt tcaaagaaaa
    21841 gcaggatttc agaggtgcgg cttatacgat tgcttttgat gatcctgttt tgcttcgtca
    21901 aggaaaagtt tatgcattga cgttggaagc ggatcagggg gcgggaaatg ttgcgatcta
    21961 tggaagcaag cgcctgaatg aaagttcatg ggatgatggg ttgccattgt ctatgtttgt
    22021 cgcttatccc tttgatgaga atacgggagt gttttattcc gatcttaatc tagaacttta
    22081 taatgatgac aatgctgaga aattggagcg tttctatacc tctcttgaag aagcggatta
    22141 catctacatc acttccaacc gacaatgggg cagcaccacc caaattccgg aacgatatcc
    22201 attgacggtg gaatactacc gtgccttgtt gggttgccct gttgaccaga atttgcagga
    22261 ttgctatcgc gatgcgcagc cgggtatgta cgatggagaa cttggatttg agctggtcaa
    22321 ggtttttcaa tcggaaccaa ctattgcggg aatcaccatc aatacacaat acgctgaaga
    22381 agccttcacg gtctatgacc atcccaaggt gttgattttt gaaaagaccg attctttcga
    22441 tatgatgacc gtgcgctcga ttttagggaa agtggatcta tcaaaagtta tctacctcac
    22501 agcggcggct gccagcaagg tacctggtga tctttccttg agcgagagcg agaaggaaat
    22561 ccaaacgaac gggggaacct ggagcgcatt gttttcaagc gattctattt tgaatcaaag
    22621 ccaatttctg ggcatcgtgg tgtggtacct cgttcttttc ctattggggg tggtcgttta
    22681 tccatccact cgcataatct tcagaggatt gaaggagaaa gggtatgcct tctcccgtct
    22741 gctgggaatg ctgctcctga catttctggt ctggatcttg ggttctcttt cagtgcccac
    22801 ggaacgttgg ttgatcagcg cggttttcgc gggtatgctg ggtgttaacg gctggttgta
    22861 cctgaaaaat cggaaaatga tccagcaaga attcaaagag aaccagcgat atttcctgca
    22921 ggttgaatgc atctttctcg ctttttttgt cttttttacg ttgatccggc tgggaaaccc
    22981 cgatttatgg catgagtata agggcgggga aaagccgatg gattttgcct attccaatgc
    23041 cattcttaaa agcactatat ttccccccta cgatccctgg tacgcaggag ggtacatcaa
    23101 ttattactat ttcgggtcct tgatagcagc ggtctttacc aagtgcctgg gtgttgtgcc
    23161 ttccgtcgca tataacttct ccctgtctac tttctttgcg ttcgctggaa ctgccgcttt
    23221 tggtatcggt tggaatttcc agtcgaagcg gttggaaaac caaactggaa agaagagggc
    23281 attttctccg ttccaggcag gcattgtctt tgcagtggca gttttgatca tcggcaattt
    23341 gggaaccatc agcatgatcc tgagtggcat caagcagatc gcgatcaatg ccgggcttca
    23401 aacgggaagc ggcatcttcg catccttctt gttgtggtgc aaagggttca ttttgttatt
    23461 gaagggagcg aagttcactt actatcccgg agactggtac tggatcccca gtcgaactat
    23521 tccaggggaa gcgatcacgg agtttccata tttcactttt ttgtatggcg atccgcatgc
    23581 gcatttgtat gcctacccga tcacgctcat ggctttggga ttcgccgtat ccattgtttt
    23641 tcgccagaaa aaatacgatg ggcagagaga tggagaatgg ctcttcttgc tgttgggcgg
    23701 tggattggtg gtcggctcgc tggcagcgac cagcacctgg gattatccgg tgtatttgtt
    23761 gttcggcatg cttgccgttg gatatgagca ttttaagcag caacgctatt accctgctat
    23821 cttcccacag tgctctcaga ccgttcggat cgtgatttct tgtgcgcttt ccttgttgat
    23881 ctttgctggt tcgacgtatc ttttcttcct cccgtaccat aacgcctatg gggcgggcta
    23941 taccagtatt caaggctggc aaggtaccca tacgcctctt tcttccttga tcgttcattg
    24001 gggtattttc ctgttcttta tcgtctcctg gttgcttatc gagacgcgcg attggttatc
    24061 gcaaacgccg atctcctatc tgaaaacgtt gcgctcttat caccccttct tatgggttgg
    24121 agggttcatc gtcgttggaa cgctgatctg gctgctgctt aagggagtgg tgatcgctct
    24181 ggtgatgctg ccgcttttgc tatggatatt cctgttgttg ctcagtcaga aactatccat
    24241 tcttgataag atcgtcttgc tcttgctgtt ggcaggttct gccatgatct tgatggtgga
    24301 agtgatcgtg ctgaccgggg atgtgggcag gatgaacacg gtcttcaaat tttacctgca
    24361 agcctggaca tttctgggca tcagcgctgc ttactgtttc gcggatcttc tcccccgctt
    24421 gtggaaaccc gcagtggaag ggtggaaaag gacctggcat gcctgcggca ttgtgttggc
    24481 ggtgtgcggt ctgcttttcc cggtggttgc cagcgtagat aaaatcaccg atcgtatttc
    24541 tgatacggtc cctcttactc tggatggaat ggcttatatg caaacctcgg tttactggca
    24601 ggatggggct gtgatggatc tggagcagga ttaccaggcg atccgctgga tgcaggaaaa
    24661 cattcagggt tcgccggtga tcgtggaggg cacatcttcc ctctatcaat ggggaaaccg
    24721 ttattcgatt tacaccgggc tgccggcggt gatcggctgg gactggcatc aacgccagca
    24781 aaaagcaatc ttgccttcgc agtggatcac cgatcgaatc gcggaggtgg aagcctttta
    24841 caacacgacc gatgggaaaa ctgcccggaa tttcttggcg aaatatcagg tcaggtacat
    24901 tcttgttggg caattggagc gcattcgcta ttccgaagag gggttgaaga aatttacaac
    24961 gtatgaagga atctattgga aaacggtttt ctcatataaa gatacaatta tcctggaagt
    25021 gatagagtag aacatggctg tgcaaatcgc tacctggtat cttcttatgc ttggtctggg
    25081 attgctcaac tacccggtta ccagttcttt gcttccgaag ctgaaaagca gagggtatgc
    25141 attttcgaag atcatgggtc tgcttttgtg gggttttctg tactggatca cgaacatttt
    25201 tcaagtgctg cccaattctt tcatgggtgc ggttgtggct ttagcgctta tcgccagctt
    25261 gaatttatcg gttttcattc gcaagaaaga agaactgaaa agggattttt tagaagataa
    25321 aagctacatc gtgatggtag aactgctttt cttcgtagct tttgtcgttt ccagtatcct
    25381 gcgcgcgatg tccccagctt taacaggaac agaaaagccg atggagctgg cattcatcaa
    25441 tgctattctc gagaccccca agtttccccc cagtgatccg tggttgtctg gttatgccat
    25501 ttcctactat tattttggct atctcatggt agccttgctg gccaggatca gcggcgtggg
    25561 agggggagtg gctttcaacc tggggatggc agtgtgggtg ggcttgatct gtacggcagc
    25621 ttatggaatt ctgtatgacc ttatttatgc gtactttgcg cataggaaaa ataaacccca
    25681 aaaagggaaa aggaagctgc tgtggttaag cctcttggct cccttttttt tactggcagt
    25741 cagcaatgcc gaaggaggct tggagatgct gcatgcttct ggggcatttt ggggcacaga
    25801 tgcacagggc aacccgcaat cgaatttttg ggaatggctg gatattcagg aattaaatga
    25861 gccgcccgcg gttgatttcc aatggattcc tcaacggatc ggtggaacct ggtggtggcg
    25921 cgcttcgcgc gtgatcagtg attacgatgc ggctggtaat tttcgtgaag tgatcgatga
    25981 aattcccgca tttacgtttt tcttggctga tctccatccc catgtgctct ccatcccatt
    26041 cttcttgctt atcactgcag ccagtttgaa cctgcttatg gggggaggaa tgtggttgct
    26101 gcatgcgcaa aattggaagg atatgctgaa aacatgggaa ttttggattt ctgcgctcct
    26161 tttcggcagc attttcttct tgaacacctg ggactttcct gccagctttg gactgttttg
    26221 tctggtgttc ctattggctt tgataaaagc gaatggctgg agctggcaaa cgtgcctgga
    26281 gttgacctgg aaagtagttt tattagcctc aggttgcgtg ttgatgtatt tgccgttctt
    26341 tttgggcttt tcttcgcagg ctgggggggt gttgcccagt ttgctctatg caacccacgg
    26401 cgtccaattc gtggtgatgt ttttgccttt catttttctt ttgtcaatct acttgatttg
    26461 gatcaatcga gggcaaaaag cgcccgacaa gaagatcgtg ccgtattttt tgctctgtat
    26521 tgccgggcta tgtgtgctca gcttgctctt tccattttcc cggcaattat cggtttttat
    26581 ctggacacgc ttacaggaat ggctggggag attcgcagat caactcaatc aagcgatcca
    26641 gcaggcgcgc tccttcgctg ctgtttatgg ggcgcttgat ctgcaatcgt tgatgaaaga
    26701 aaccgttacc cggcgcttgc gggactccag cgtgctaatg atgctgtctg ttttcttata
    26761 tttggcgatt cgctttttct ttcatgagcc caaaattgaa aacgagggga gggatgagca
    26821 ccatgcgaac gaggcattcg cgcccacatt tgttgtgttg ctgattgtgg tcgggtgcgg
    26881 gttgtgcttg ataccggaat ttttcttctt ggtcgatgtg ttttcggacc gtatgaatac
    26941 tattttcaag ttctactttc aggcgtggat cttgttttcg ctggcagggt cgttcatcat
    27001 agtggtcctt tgggatgctt tcagaaaatg gcaaaaagtc gtttttcgtg cagcgtctgt
    27061 cttcatcctg ataatctgct gtgcctactc tttcttcatc taccgtgaac gcattcgcta
    27121 tacccgcgta gaaaattgga cattgaatgg aagcgcatat cttgcatgga gcaatcccgc
    27181 ggatgccgca gtaatcgaaa aattgaaaac gcttccctat ggggtggtcg cagaggcagt
    27241 ggggggcagt tatagcgggt atgcgcgaat tgctaccatc agcggatatc ccactgtgct
    27301 gggttggcca gggcatgaag cacagtggcg aggtggatat gaggaaatcg gttcgcggca
    27361 aacggatatc aaaaccctgt atgaaacgtc tgattggacc accgcgcaat atgttcttga
    27421 ccagtatcgt attcggtatg tggtggttgg gagtttggag cgcaccacgt atgttgtgga
    27481 agagcagaaa tttgctgaaa accttctact ggtgctagaa aaaaatggga ccaatttcta
    27541 tgtggttcct tagaaagtaa tggcatgaag caattcaaca aggtcaaact tactcattta
    27601 ttctatgccc tcattctgct tgtcgcagtt tttctgcggc tggtgaattt gggtaccgtc
    27661 ccgttaacca ataacgaggc gcaacatgcg ctctgcgttt tggatgcgca acgagatgat
    27721 tgtgaaaatt attctcggtt gtatatcttc ttcacagata ttttctttaa aatttttggc
    27781 acgaacaacg gatctgcccg aatgctaccc gcgttgcttg gcagcacgat cgtattgatc
    27841 ccattattgc tgagttctgt agtggaaaga aaaactggca tccttctggc tatgtgtctg
    27901 gcgttggatc ccctcttaat gcaaacttca cgcagcgctg atgacaccat gcttgggatt
    27961 gtgtttgtcc tgtttttcct tgcctttttc ttgcaccgaa aggtaaccgc cgcgctgatt
    28021 acctttgctt tcggcttatt gggtgggagg tccttttgga tcggtttggt cattctcggc
    28081 ttggcaaccg gcgttacctg gttattaacg cacagagaaa tgcgggcaga gttgaacgcg
    28141 aagataggga aggtgcggct tggaggaaag gaaagcaggt atctgttctt gacccttttc
    28201 ctgttctgga taggtatcag cacccgaatg ttcaacgacg tgggcgggct gctatcaccg
    28261 cttaagtcgc ttctcgatat ttttcctgga gcaggaactc agagggggac atcagcgttt
    28321 tcaactgatg ttcgtttgat cgtctttctt ttttattctt tctatgccat tagtttcagc
    28381 atttctgcgc ttttccattc gaattctgac cagaagcgga gggatatctt cttactggtc
    28441 tggatactct ctggcatact gatctttctc atcccacagt ttccatttag ccaagcggcg
    28501 tgggtcagca ttgcgatgtg ggtgttggct gccggcagaa tgatgcaaat aggatccagt
    28561 gcgtggcgtg aaagaaagcc gctcatcctt cctgtgctgg tagggctgac cattttggtg
    28621 tttctctcat ttcaattgtt gcgccttcaa tatcttttgt ctgccggact ggatctttct
    28681 ctaaatctca cattgttgat cacgccaatg ctgctgtgcc ttcttttcat cctcttatac
    28741 gcttacggct ggtcaaaacc tcgtgcagtg caagtgttat ccatcttgtt cataatctgt
    28801 ggggctgttg cactcctgcg caatgccaac cgctcatcca atcttacagg cacccatgca
    28861 tacgaacttg tacaagagga ttcttttttc aagaatcagg atatcctgat agaacgaatt
    28921 gagaattaca ggaacgcaag ggggatgcca caaggcgaag taaccattgc catttgcatg
    28981 gatgaacctg gcgagagatg ggaatgggtt ttacgggatt actcggtgac ggtgaccgat
    29041 gacatttcag aggagaccag caatgattat gatatgatga tcatgcgtgc cgactctaca
    29101 caggatttcc cccggcacga aagtgaaaat ttggttcttt ccggtgaagt tgcctgggtt
    29161 cgtgatgatt tttctggatt tcttcccgag gagatattag aatggttaat atatcgcaca
    29221 gctaccctta agacgaccag ttatgctgtg tggtacaagt cactttaaat cgggaaggat
    29281 ctatggagct gaacaacgcg atcaccattg atggaccggt agcttccggt aaaaccactg
    29341 taggaaaaca attggcagaa aagcttggct atcaattttt ggatacaggg attatgtacc
    29401 gggcagttac attggcggcg cttgaggatg gaattgacct gcagaatgag aatgcggtca
    29461 gtgagaaagc cgaacaaata agaatcgaaa tcctgcctcc caccaaa
//
LOCUS       DAOSDS010000018        29360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_753_length_29360_cov_4.461423, whole genome shotgun sequence.
ACCESSION   DAOSDS010000018 DAOSDS010000000
VERSION     DAOSDS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 29360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29360
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_753_length_29360_cov_4.461423"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..876
                     /locus_tag="PLL88_03770"
     CDS             301..876
                     /locus_tag="PLL88_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HOJ00711.1"
                     /translation="MNTTNGNKKELSPEQREGLLRALKARFEKHMNRHAGLEWAQIQA
                     RLDANAEKLWSLHEMERTGGEPDVVGYDEKTSEYIFYDCSAESPPGRRNVCYDREALD
                     ARKANKPEGNAVDMAAAMGIELLTEEEYRGLQKLGNFDAKTSSWVKTPSDIRKLGGAL
                     FADYRYGNVFVYHNSAPSYYSSRAFRGSLRV"
     gene            complement(1138..2271)
                     /locus_tag="PLL88_03775"
     CDS             complement(1138..2271)
                     /locus_tag="PLL88_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HOJ00712.1"
                     /translation="MSNKISTKAIPMLLAVAVLAACTTPIGNADQQPDAMSGKLTGTV
                     TYRERIALEADAVIEVQLLDVSKADAPAEVIATQTIKSDGKQVPIPFELDYDPAEIDQ
                     KSSYSLQARILVDGQLRWISEQVYPVLTQGQPTNAVEIIVNRVPGGDVGIPDQEWTLQ
                     SMIIDGTEIALESGVSTTIRFAADGKYGGSGGCNSYTGSYTLQGNTISLGAAAATLML
                     CQTGMDQETNFFAALETISSFEIAADSLQLSSADGKTILTFGAGAESQSITDVIWKWT
                     ESLDNNDKATTVSEPEKYTIEFKSDGTIALRADCNTGTGSYQLDDNQLTIILGAITFV
                     GCPSASLDRQYLDELAEVASYLFDGQDLILEWKFDSGTMRFSR"
     gene            complement(2872..3561)
                     /locus_tag="PLL88_03780"
     CDS             complement(2872..3561)
                     /locus_tag="PLL88_03780"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOJ00713.1"
                     /translation="MHQSSSGDERLVVKAGGLTLLILALFALMFFLPAGTWKYWEAWV
                     YMFLIFSWMMAVLGYLVKKDTGLLHHRLQYKERRTEQRAIFRFIHPVFFIMYLLPGFD
                     KRFGWSDLPLGVVVISDLLVLIGYGVFFLVIKENSYASRIIEVQADQQVISSGPYAIV
                     RHPMYFGMLVLFLFTPLALGSWVAVIPALFLIPFMIFRIKDEETSLQNHLAGYQEYMQ
                     RVKYRLVPGIW"
     gene            3725..4372
                     /locus_tag="PLL88_03785"
     CDS             3725..4372
                     /locus_tag="PLL88_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00714.1"
                     /translation="MVDAITQFERLLTSEELAQLQSMKSPFRIQTFLDSTLYPPGERN
                     RSPSEVLRERQAHCLDGGLFAAAALRRIGYPALIVDMQPEPGTDDDHVLAVFQEFGCW
                     GAVAKSNYSGLRYREPIYATIRELVLSYFDDFFNVHGQKTLRTYTAPIDLAAFDDWQW
                     MTEASGVDRIEELLKRADLTALITEEQARYLSPVDERSFKAGTLGINPKGTYKPQ"
     gene            4619..5782
                     /locus_tag="PLL88_03790"
     CDS             4619..5782
                     /locus_tag="PLL88_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00715.1"
                     /translation="MDRKRIAILSLSLLTIIYTDTIAPLVQQMCAAFPQISPTLVKMT
                     VTLPALMTIFFGLIAGQMVKVISKKAVLAIGLTMYSVGGVAAGYVHNFTIHLVYRGLV
                     GAGVGLITPIITSLVADYYQGKERADMIGYNFAVSHFAAVITPALASYVGTVSWRNAF
                     LIFLVVPLVFVVAMILLPGASRNNQPGKIKEKAPITTRAMQCAVIAMLLMVFFFVVVT
                     DLSYLLNTKDVVSPFLATFGLSTFTFGFAIAGLAFSWIYLKIQRWVIPSGFVVCGAGY
                     FLVTFSQTNALILAGLLSIGLGMGMLISLLVLQTTNSVGKADSTAAAALTNAGFSTGI
                     FLSPFFYEKLPSILTGMERIEFNFFLAGGIFLLAGVITLTGLIVRRKKLAIQA"
     gene            complement(5840..6448)
                     /locus_tag="PLL88_03795"
     CDS             complement(5840..6448)
                     /locus_tag="PLL88_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00716.1"
                     /translation="MKKTPLHPIALCCLFACLVLVAGCSAKINSIPLTSAFPDATLEN
                     ILSAFQAPAFTFSESRAYKGSELITGVSPDGMSSLQVVVSGEDVLAARSLIYLSIDAS
                     AEYANAQNEHLILLISTIFGDSWPAKEWIITATNSIAQRNAASLMGGASVTTERETGA
                     GVMPVSLYVRMDENNQGLVMGVVIGDWLDDISYDPARNVWEP"
     gene            6707..7663
                     /locus_tag="PLL88_03800"
     CDS             6707..7663
                     /locus_tag="PLL88_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ00717.1"
                     /translation="MKNIFLAVTLFVLNSLNAFSLADEGSQPTAYDLIAEVNAYRAAN
                     GYYQLAPNSLVMAAAQTHAEWIVATGQGGHIGAGGSDETMRVSWTGYGGGAEIHCDEN
                     WASGRTVSDVVYGAWSDWTHQEVMLNAWGNRYTDVGAGVAARGNGSYVFVLNVCMVVG
                     QGSSGSVPGATSNPLATQDLSNYVYGVTKATPLTDGTIKHKVLYGQTLTTIAEAYGVS
                     IETLQALNDMTADDTTIWVDQELLIQIGTGTEEAQESPTPSAAATTRPTQTASVSSST
                     ATPQSSRENSRVGLSAKTLGILLVGISVVGLVVVVYLFFIKK"
     gene            7955..9082
                     /locus_tag="PLL88_03805"
     CDS             7955..9082
                     /locus_tag="PLL88_03805"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOJ00718.1"
                     /translation="MAKYIGVLTAGGDTPGLNAALRALGKSALGEYNLQLIGFYDGFR
                     GLMNDRSVILDEQKLSGILTFGGTVLGTSRDKPHRMPVGDKLMDMTDAMLEVYHKHHL
                     DALVCIGGGGTQKNAYRLMQKGMNVITIPKTIDRDIAMTDATIGFDTALGIATEAVDR
                     LHSTAHSHHRIVLVELMGHNAGWLALGAGVAGGADVILIPEIPYEPARIAEAILARVH
                     SGKRFSIVAISEGATSKKAVAELDALTQEKKNAKNKEEKRKVGEKIENFLHSQSNKTM
                     LLAQELESLTGLESRVTILGYVQRGGTPSARDRLLATELGTTAASLLAKEKYGCMVAV
                     HEERIEAVPLEKVVDRRKVVPLDHSWVKTAKRVETCMGDDI"
     gene            complement(9137..9781)
                     /locus_tag="PLL88_03810"
     CDS             complement(9137..9781)
                     /locus_tag="PLL88_03810"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOJ00719.1"
                     /translation="MLKQSKKINGFLVLGFAVILLSACTGGANAEPTQDPNVVFTQVA
                     ETVMVSMTQTAIAQPSATPQPTATSMPTLPPTPTVDPNQPTATAQVSNQPAYPTATTQ
                     YYGDAARWNTQTPIDGTVLTRSQNFAFHVCLSNVGATDWTTKYYLQYVSGYQLWSSAT
                     KFYIGETVEPGGKWCFDLPSIAPDKVGEYTTRWYLKNNTNESFSEVYFHYYVAQ"
     gene            complement(9896..11701)
                     /locus_tag="PLL88_03815"
     CDS             complement(9896..11701)
                     /locus_tag="PLL88_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00720.1"
                     /translation="MPVNTFEEIDYSREGTTGKIFLRIFKFMKPHWGLMLVFLVAVIL
                     TSVMDAYTTFLTKRIIDEGILSGQVGILLNILYQYALIVLFEAGTVFTFVYTVSTVGE
                     KVRFDLRQKLFNHLQNLSLSYYNQTPVGWIMSRVTNDTERVAELVTWGILDSTWGIVN
                     IATSMYFMFKINTRLAFIVIAMLPLLLYVGIKFQTRILRQYRVVRKINSQITASYNEN
                     ITGVRVIKALGREKENLKEFGDLTGEMYTSSYKAAWISALFLPTVQLISSLALGGVIW
                     YSGAQATVGGISIGGMQAFLSYITFMMWPIQDLARVIAEMQRAIASAERIFTLIDTPP
                     DVKDKPTAFDPGSLHGDIEFEKVSFWYEDGSPVIKELSFTVKQGETVALVGSTGGGKT
                     TIVNLICRFFEPKRGRILIGNKDYTDFSLSAIQSRIGVVLQTPHLFSGTIKENIAYGR
                     LNATHQDIEAAARLAGAHDFITKMAHGYDEPVGEGGVLLSVGQKQLISLARAVLSDPE
                     IFIMDEATSSVDTLTEAALQKGIDSIMVNRTTFIIAHRLSTIKNAGRILVIENGQISE
                     MGTHKELIQKQGKYYHLYTQQFRQEQQKKYALFEE"
     gene            complement(11691..13499)
                     /locus_tag="PLL88_03820"
     CDS             complement(11691..13499)
                     /locus_tag="PLL88_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00721.1"
                     /translation="MTVKYQTLDFKKILNEKRLSGLWLLMSGYQIQYIGAMIFQIIAA
                     LAKSGTYLLLEYFVDNFLLAQSATVSVVAVAIGFILLFLFQGLATFFSQKDAAQVAEG
                     TTRRLRDYLYDHIQNLTFAYHAKTDTGDLVQRCTSDVDAIRRFYADQAIGVGRIIILF
                     LVNFIALLRLNGKLALYSIIVVPIVLFISIYFFQKVTKAYEAYQEQDAILSTTLQENL
                     SGVRVVKAFARQPYEIEKFKRDNFEKYNRGRKLMALHSLFWPISDIFCGAQLLFGYFI
                     GAKMAMDGVITVGTYMAYAGLVVWIIYPLRNLGRMIVQMSNGLVSYDRLYKIIKEDRE
                     PLDQGSITSNTRIKGDISFLNVSFHYEDGSEVLHDITFECKAGMAVALLGSTGSGKTS
                     LVNLLPRFYDVTEGAILLDGSDLRLYTRRFLREQIGIVEQEPFLFSRSIYENITFGIK
                     EKVTQLQVEEAAKMAAIHDVILSFPQGYKTLVGERGVTLSGGQKQRIAIARTFIKDPR
                     ILILDDSTSSVDAETEAHIQSAIQKLMQNRTTFIIAHRVQSIMNADLILILDKGHISQ
                     KGTHQELLKQAGVYRNIFDIQTRIEEDLQKEIDRAS"
     gene            13732..14880
                     /locus_tag="PLL88_03825"
     CDS             13732..14880
                     /locus_tag="PLL88_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOJ00722.1"
                     /translation="MIRMKEGSGIVVANKKIRMMIPGPVEVHPDVLKAMGSAVEPHYG
                     DAWVAKQQRVTEILKEIFNTAGDVYLLVGSGTCAIDAAIGSCLQGGEKILVGTNGYFG
                     DRLVSIAKHNGLEVIEVKATWGEELDADEIERALERDPSIKAVAVVHSETSTTVLNPI
                     QDIGPIVRRHGQLFIVDAVSSLGGVPFSMDEWCVDACATATQKCLGAPPGLGPIAVNE
                     RAWKSIDRLQTGEHGWYTDLRVWRQYAQEWGDWHPTPVTMATNNVNALLVSLTQLKAE
                     GITKRMERYRSLALQLREGMRKAGIAPFTPDEKLNPVLTAGVAPSGVNSAEIVRYFLQ
                     EYAIQISGGLGALKAGIFRIGHMSPVMTSEDIELVCEALASFCRTRNL"
     gene            14974..16092
                     /gene="ugpC"
                     /locus_tag="PLL88_03830"
     CDS             14974..16092
                     /gene="ugpC"
                     /locus_tag="PLL88_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOJ00723.1"
                     /translation="MANVIFDHVVKKFNDVVAVNDLSFVLDDKEFLVLVGPSGCGKTT
                     ALRCLAGLEEVTSGEIKIDGKVVNDVPPKDRDIAMVFQSYALYPHMTVFDNMAFGLKL
                     RKVPKDEIKRKVEEAAKVLNIETLLKRKPKELSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVQMRTEITKLHQRLQTTFIYVTHDQMEAMTMGTRIAVLNHGILQQIDTP
                     QMLYDKPNNLFVAGFIGSPSMNFFNGKIEKKDGKVMLKAESFEVEIPKEKIKVYEHLA
                     GKKIIFGIRPEDVHNPEYLPARIKPAIIKSIVDVTELMGNEIFLHLKPSEKDAVYVAR
                     VDPRTRVKVGDVIEVAFNMENIHVFDPSKDAENPVAVR"
     gene            complement(16280..16948)
                     /locus_tag="PLL88_03835"
     CDS             complement(16280..16948)
                     /locus_tag="PLL88_03835"
                     /inference="COORDINATES: protein motif:HMM:NF018964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HOJ00724.1"
                     /translation="MKTVYRHIMFLAFILVILIAGIRFCLTHTFIDLEYAYPAFPDDT
                     YGFSRDTRTVLAYQSVDYLLGKLSDADYQALATPDGKPLFNQRELSHMRDVRDLTHIV
                     LAVWYASIAVMAAGIVLALKLHWQTHLLEAGKLASKIIFFFIVFVLLAVLLNFDRLFT
                     IFHSLFFEGDTWLFYPSDSLIRLFPTPFWVNVFITVGIISFTLAFFLHYACGSLLKKK
                     ERVE"
     gene            complement(16945..18504)
                     /locus_tag="PLL88_03840"
     CDS             complement(16945..18504)
                     /locus_tag="PLL88_03840"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOJ00725.1"
                     /translation="MRTRIYFAIFGAGLVSLAIEVSASRLLSNYFGSSNLIWACVIGS
                     ILIYMTIGSILGGKWADRSPQLELFYRIITWGAFSTGLIPSIAKPVLRSASLAMDTLS
                     IGPLILAFAAVLALFSVPMVLLSMTSPFAIRLLVDEARTSGNISGKVYAISTAGSFIG
                     TFLPVLVLIPTVGTYKNFLILASFLWLVGFWGSCTTPLGFRKTLIKFGWMPLVIALSA
                     WFGLQGTDKISTGLIYEKESEYNYIQVQQINGYNLLRLNEGQGIHSIYHPGTLAYGGT
                     WQQVLVAPFFQDAPYSPQDLRSMAILGLAGGTSARQAVSVFPDVHIDGFEIDGTVIEV
                     GYDYFGMDVPQLTVFAQDARLGLRAMHKEYQLISIDAYRPPYIPAHLTTLEFFQEVYD
                     HLSADGTLVLNVARIGDDRRLVNALATTLGRVFPSVYISDVPDTFNTMLYATRSATSV
                     QNLYNNYLFLDASGNFPALLRESMETAILNLQPLGASDLVFTDDRAPVENLVNTMLID
                     FYRAGGVETLR"
     gene            18546..20141
                     /locus_tag="PLL88_03845"
     CDS             18546..20141
                     /locus_tag="PLL88_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOJ00726.1"
                     /translation="MKEIKAEVCVYQIVSVEQMRALEHEADSRGLSYAQMMENAGKAA
                     ATLINQRFGKRKGKSIVGLVGSGNNGGDALVALNQLQCQGWDTHAYLIKERKEHDLLV
                     EQYLAGGGNVDRFSNDENHSLLDNLTKHCHFLLDGIFGTGVRLPLRGEIEEVLQHLRD
                     AEKLPYIIALDCPSGVDCDSGEADVAAVHAQWTISMAAVKRGLLEFPAFEHVGELSVV
                     EIGLPADLEAWKGGREFCMDESAAAAMLPARPVQAHKGTFGTAVIFAGSRAYPGAAYL
                     AAKAAYLAGAGLVTLASIAEVRQAIAGTLPEATWYLLQTQDEGFGWDETTSLPAVLQK
                     ASAILAGPGWGTHPSAAILLERLFDIMKENPRTPLIIDADGLNLLSQHVELFKKLPAN
                     CILTPHPGEMARLSGLAVEEIQKDRQTIARTYAKKWNATLVLKGALTVIADPCGRLVV
                     NPVATPALAKAGSGDVLAGMLAGLAAQGIDGLDAACMACWMHGRAGMLAERAVGHSAS
                     VMASDILRSIPWVFRILLKRKAG"
     gene            complement(20179..20505)
                     /locus_tag="PLL88_03850"
     CDS             complement(20179..20505)
                     /locus_tag="PLL88_03850"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOJ00727.1"
                     /translation="MSDNDNFGSFFSGLLLGGIAGAVVALLLAPQAGEETRKLVKEKS
                     IELRDKVANYAEEVLTQAEKTVGDVSAAIDDTMQSASEVAEKGQVILESKKEKTPSTK
                     KIAKSE"
     gene            complement(20533..20973)
                     /locus_tag="PLL88_03855"
     CDS             complement(20533..20973)
                     /locus_tag="PLL88_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00728.1"
                     /translation="MSESSQPLPGTPPADPNANMIDKRKLVWGIIIFLLVMAASIYGI
                     YSLVKAGPDATSQVRDIFIIVLAFESFIIGVALVILVIQLAALTNMLQNEIKPIISNT
                     RETIDTVKGTADFLSKRAVKPVIAANSYMAGAKKLFEILGILKK"
     gene            21232..21825
                     /gene="pth"
                     /locus_tag="PLL88_03860"
     CDS             21232..21825
                     /gene="pth"
                     /locus_tag="PLL88_03860"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOJ00729.1"
                     /translation="MTGEKHLIVGLGNPGNQHEKNRHNIGFMVVDALAKRWGISMKRN
                     RKNALTGEGTYQGIPVVLAKPQTYMNNSGLSVGPLSRLYEILPENLLVIYDDLDLAFG
                     RIRLRASGSAGGHHGMESIIEKASTSEFPRLRIGIGRPQHEGEDVIDFVLTGFHGPDK
                     QKLKTVLSQAADAVETYLQSGIEIAMTQFNGVFLDEE"
     gene            21822..25208
                     /gene="mfd"
                     /locus_tag="PLL88_03865"
     CDS             21822..25208
                     /gene="mfd"
                     /locus_tag="PLL88_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOJ00730.1"
                     /translation="MINIRFHELDGFTHALSAVRGGRPEGALGLVKPVRLPLAMTLQK
                     ELKVPLLYVTGQMNRAQVLMEEAQFWKQNENAELYAEPVPLFYEDSDWGANVRSNRIA
                     VLEKLSRAWVPGKEQNAEDVLIIAPIKALMTRTIPRRDYLMARKEIKVGAEHDLEKLT
                     KHILRLGYEFQEVVSGVGQFSRRGGILDVWLADQAHPLRIEFFGDEVDSMRVVDETSQ
                     RSMEQLNACVILPAREFILPEDTGEDIRHYHERDICVLHPNATSLFSYLPKGSLVCLD
                     DQDILITVSEEVEKDALELREQLIKDKRLEKNFPAPYDSWSELYDQMLSFNPLNMSFS
                     GVETLQLFRQCIQPAPRFGGKMDALLQFLEEEVEENHSATMVSRQSERLQKMWQERAA
                     GKEKQVTFLNQYLSAGWKVIYPDGTVEYCITDEEIFGWQPAIRAKRRSSQQLTVFDFG
                     AFVPGDYVVHFEYGIARYQGLVMRTLEGVEKEYLLLEFAENSELYVPVHQADRISLYI
                     GPDARPPKLSALGSPNWEGIKNRIKQAVHYVAGDLLELYSKRQSVEGYAFHEDTDWQR
                     ELEASFPYEETEDQLQAIADVKRDMERAKPMDRLLCGDVGYGKTEVAVRAAFKAALDG
                     KQAALLVPTTVLAQQHLDTFRQRLAPFPVQVEMLSRFRTEAEQEEILLALEAGEIDII
                     IGTHRLLQKDVAFKDLGLLIIDEEQRFGVMHKEFFKKMREKIDVLTLTATPIPRTLYM
                     ALSGIRDISMINTAPTDRLPVTTHVGGYDSELVRRAILREIDRGGQVFFVHNRVQTIS
                     TFAEQLRAVVPEARIAVAHGQMPEQQLASVMYQFTEQKVDVLLSTSIIESGLDIPNAN
                     TLIVDRGDTFGLAQLYQLRGRVGRGTQKAYAYFFFNSRGNPTVEGLERLEVIAENSQL
                     GSGYTIAMRDLEMRGAGDLLGTQQHGHIAAVGFHLYTRLLAEAVKNIKRKQGEEPLAE
                     RLKEVLAEPELRPFVSVDLPLPTSIPVDYIPDAGVRLELYRKLARVTSEKGFEEIGEE
                     FRDRFGASPQDVSNLIWQSKIKLMAEKAGLYSIAVEAQQLVMRYPPLPKNIKRRSLVP
                     LEPYWRSGKNEYWLCIDMKGKEWMSQLEDALENLIEKGKQ"
     gene            25223..27280
                     /gene="uvrB"
                     /locus_tag="PLL88_03870"
     CDS             25223..27280
                     /gene="uvrB"
                     /locus_tag="PLL88_03870"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOJ00731.1"
                     /translation="MDFHLKAPYQPMGDQPEAIRQLVEGLQRREKHQVLLGATGTGKT
                     FTIANVIQQVQMPALVLVHNKTLAAQLYAEFKEFFPENAVEYFVSYYDYYQPEAYVPR
                     QDLYIEKETEINEEIERLRLAATTALVSRKDVIIVASVSAIYGLGSPEAYRNIVIRLQ
                     VGEISRRNALLRRLVEQQYTRNDIDLHPGVFRLRGDTLEIYPAYEKKKVIRITFFGDE
                     VEKITMLNPVTGEILEDLESVEIYPAKHFITEDDLLKKAIVDIETELEEQATYLEKHN
                     KLLEVQRLRQRTNFDLEMLREIGYCSGIENYSRHLDQRPAGSAPWTLMDFLPADYLLI
                     IDESHMTIPQVRGMFNGDRSRKGTLVEYGFRLPSALDNRPLTFDEFEERMGYTIYTSA
                     TPGPYEMGKVKHVIDQIIRPTGLVDPEVEVRPVMGQVDDLVHEIKLRVEQGERALVTT
                     LTKRMAEDLSLYLGELGMKVNYLHSEVETLERVGILRDLRMGVFDVVVGINLLREGLD
                     LPEVSLVAILDADKQGFLRSETALVQTIGRAARHVHGKVIMYADSVTDAMKAAIDETN
                     RRRAKQERYNKEHNIQPVSIIKEIYDITERLSDQMVTDTSGGYDAESGNLVHIPKDAL
                     QQLIQETEQKMQAAAKELDFERAAVLRDQVYELKRILVEESNVAPWKKAMLLAGEDEE
                     EKD"
     gene            complement(27277..27570)
                     /locus_tag="PLL88_03875"
     CDS             complement(27277..27570)
                     /locus_tag="PLL88_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOJ00732.1"
                     /translation="MDSSQVARMHVVVSGRVQGVGFRFFTMQSAWTHEVTGWVRNRLD
                     GDVEVVAEGRREALEAMLLDLKQGPASASVADVKVEWLPYQAQFTAFEPRETL"
     gene            complement(27577..28536)
                     /locus_tag="PLL88_03880"
     CDS             complement(27577..28536)
                     /locus_tag="PLL88_03880"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOJ00733.1"
                     /translation="MTEENRDTPFEQILTELVSEEQPFSPKRLRAFSDLDDSALLKLE
                     QIWSSVPLKRKLSLLEDLEDLMEADTLISCDALAKFAMRDDEPRVRSQAIHLLWDCED
                     PAFIPDFMHLLENDPAELVRASAASALGKFILLGELEEIPGEKAAPVVGKLLEVVQRK
                     PAGEIQRKALESLGYSCNESIPGLIAQAMQRNDPQWTASALFAMGRSLDERWEKTVLE
                     HINHPDSSVQIEAIRAAGELELSAANLFLFELLDEEDADNELLYHIYWALSKIGGKGV
                     RERLEQELQEAVDEDLMDVLDLALENLDFTEDSEGFDLFDVEE"
     gene            complement(28533..>29360)
                     /locus_tag="PLL88_03885"
     CDS             complement(28533..>29360)
                     /locus_tag="PLL88_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOJ00734.1"
                     /translation="YEQNKWKRLGVLLAGALTNLLIGIVLFTIVFSNTGKAITDQVLI
                     QQVEPGTPAEIAGFMVDDRILMVNDVTIDSIDLLTATVSENLGKQIAVVVERAGEQLT
                     LLATPRAEHPADQGPLGIVLAYPVEPIGFFESLSSAFRMAKEQGELLLKIPSKLISGE
                     IEIADVRPVSPKGIYDIYSQVREEEEASGADQKSRVLNILWFFGVVSVSLGYSNLLPI
                     PALDGGRILFLLPELITGKRVPEKFEGAVNLIGFTILIIVMVFVFIQDFTNPIVLPR"
BASE COUNT         7482 a         7048 c         8143 g         6687 t
ORIGIN      
        1 ggtagagcag gggactcata agcccttggt cgtgagttca aatctcaccc tcgccatttt
       61 ttgttgcatg ctacaggtgg aatgagatag aaaaaacaag ccagaatctt catatagtga
      121 ttggttgttt tgcgaaagga taaaaaggct tagcagggta acaattaata aatagaatga
      181 atccctttgc gacaatcgaa cagttaaccg atgcgctgga ggtgtcggtt ggtgggcttt
      241 gaaataaatt caggcaatgc tatgcttgac atgcctgttc agcagcgcta gtataaaagt
      301 atgaacacca cgaatggcaa taagaaggag ttgtcaccag aacaacgtga aggactgctc
      361 agagcgttga aagcccgttt tgagaaacac atgaaccgcc atgcgggtct tgaatgggct
      421 caaatacaag ccaggctgga cgcgaacgct gaaaagctat ggtcgctcca tgaaatggaa
      481 agaactggcg gcgagccgga tgtcgttggt tatgatgaaa agacgagcga atacattttt
      541 tacgattgtt cagcggaaag ccccccagga cgcagaaacg tctgttatga ccgtgaagcg
      601 ctggatgcaa ggaaggcaaa caagccagaa ggcaacgctg ttgatatggc ggctgccatg
      661 ggcattgagc ttttaacaga agaagaatat cgagggttgc agaaacttgg aaatttcgat
      721 gcgaaaacgt cgagctgggt gaaaacacct tccgatatta gaaaactcgg cggcgccctc
      781 tttgctgatt accgctacgg caatgttttc gtgtatcaca acagtgcgcc ctcttactat
      841 agttccaggg cgttccgtgg ttcgctaagg gtctaaattt tcgcagacag caaaaagagg
      901 gtattctttg ttgtgcgttt cagacaagtg ccataggttt aataagcgga ttcaaagaac
      961 actgggcgct aagtttttca aagtgtcctg ttaaatgatg ccaatggcat tctcgccaaa
     1021 gctgagagaa tacacaggca atacgcctga tgcggttcac ttccttccag gcttggctta
     1081 cgggcatgaa ttacccctgc cagaaaatag aatgcctgat gaagcttctt gttgcggtta
     1141 ccgactgaaa cgcatcgtgc cgctgtcgaa tttccattcg aggatcaaat cttgaccatc
     1201 gaacaggtat gacgctacct ctgccagttc atcaaggtat tggcggtcta aggatgcgga
     1261 agggcaccct acaaacgtga tagcgcccag gatgatcgtc agttggttgt cgtcgagctg
     1321 gtagctgcct gttccggtgt tgcaatctgc ccggagtgcg atcgtcccgt cacttttgaa
     1381 ttcgatggtg tacttttccg gttccgagac ggtcgtggct ttatcgttgt tgtcgagcga
     1441 ttcagtccac ttccagatca catcggttat cgattggctt tcagcacctg cgccaaaagt
     1501 cagtatcgtc ttgccatcag cagatgacaa ctgcaggctg tctgcggcga tctcaaatga
     1561 agagattgtt tccagtgcag cgaagaagtt cgtctcctga tccatgccgg tctggcataa
     1621 catcaacgta gccgctgctg caccgagcga gatggtgttc ccctggagcg tgtacgatcc
     1681 agtgtagctg ttacacccac cactgccgcc gtactttcca tctgcggcaa atcggatcgt
     1741 cgttgaaaca ccgctttcca gagcgatttc cgttccgtca atgatcatcg attgcagcgt
     1801 ccattcctga tccggtatgc caacgtctcc gccgggcacc ctgttgacga tgatctcaac
     1861 ggcgttggtc ggttgacctt gcgttagcac agggtaaact tgctcggaaa tccagcgtaa
     1921 ttgcccatcg accaggattc gggcttgcag cgagtagctt gatttttgat cgatctcagc
     1981 aggatcataa tctaattcaa aggggatcgg aacctgtttg ccatctgact tgatcgtttg
     2041 tgtggcgatg acttcggctg gagcatcggc tttcgaaaca tccagaagtt gcacttcgat
     2101 cacagcatcg gcttcgagcg cgatacgctc gcggtaggtg acggttcctg tcagtttccc
     2161 agacattgcg tcgggttgtt ggtcagcgtt gccgatgggt gtggtgcatg ctgcaagcac
     2221 ggcaacagcc agcaacatgg gaattgcttt ggttgatatt ttatttgaca tgcttcctcc
     2281 actttttcac ttgtgtggtt cgcgatatag tccatcaaaa attttcgtcc catctataaa
     2341 gctgcatccc ggcagcaggt cagcaggaat ccacgaacca cagaaacttt cctcgttcaa
     2401 agcagggtca acgacctttg ttgtcatagt gtcattaaaa tataccatgc ctcatccact
     2461 gtgctctctt tctcagtggt agagagaatc ttcgcctttc cataagggaa gcggaacccc
     2521 ttgtagttta aggtgcggaa agaaccaacg atcatggtca aactttcccc aaaggaaagg
     2581 cgatcgttct gatctctaga cctttgctat tgaagagcat gcttgaacta ttgaggaatg
     2641 acggtgttgc tgtgtttttg tttcttgagt tttctcttct ctttgatcgt cagcttcggt
     2701 tttttcctct gttccttctt gcttttgtct ttctttccag attcacccat tctgacctct
     2761 cttttgtgtg ctgcttccaa aaagagcatt ttccttaaac aagagtataa gattatgaat
     2821 gtttttgcaa ctatccgatc tccctcagta tactattgaa tgcaatacgt ttcaccagat
     2881 tccaggtacc aggcgatatt tcacgcgctg catgtattcc tgatagcctg caagatggtt
     2941 ttgcaatgag gtttcctcat cttttattct gaagatcatg aaaggaatga gaaacaaggc
     3001 agggatcacg gctacccagg agcccaacgc caagggggtg aacaagaaga gcaccagcat
     3061 gccaaaatac atggggtgtc gaacaatagc ataaggaccg ctgctgatga cctgctgatc
     3121 tgcctgcact tcgatgatgc gcgaggcata actgttctct ttgatcacga gaaagaagac
     3181 gccgtagccg atcagcacga ggaggtcaga aatgacgacc acgcccagcg gcaagtcgga
     3241 ccatccaaag cgcttgtcaa aaccagggag aagatacatg ataaaaaaaa cggggtggat
     3301 gaatcgaaaa atggcgcgct gttctgttct tctttctttg tattgaagcc ggtggtgcaa
     3361 caaaccagta tcttttttca ctagataacc gaggaccgcc atcatccagg aaaagatcaa
     3421 gaacatgtat acccaagctt cccaatactt ccatgttcct gcggggagaa agaacatgag
     3481 ggcgaagagg gcaaggatca ggagcgttaa tccacctgcc ttgacaacca acctttcatc
     3541 ccctgacgag ctctggtgca taatccctct gtaaaaaatt aatccttcgt attgcgtttg
     3601 atttttacgg tcgcaattta tcctgtgccg tatccgtaag gatagtatat acctacctgt
     3661 ttctttccag cgaccgggca gactgggtat aatctgggta aaagagcgta ccctgggaga
     3721 agttatggtc gatgcaatca cacaattcga gcggttgctt acgtctgaag aattggcgca
     3781 actgcaatca atgaaatccc ctttccgcat ccagacattc cttgattcca ccctttaccc
     3841 gcccggcgaa cgcaaccgtt ctcccagcga ggtgctgcgc gagcgccaag cgcactgcct
     3901 ggatggggga cttttcgcag cggcggctct gcggcgcatc gggtacccag cgttgatcgt
     3961 ggatatgcag ccagaacccg gcacagacga tgaccatgtg ctggcggtgt tccaggagtt
     4021 tggttgttgg ggagcggtag caaaatccaa ttacagcggg ttgcgctatc gtgaaccgat
     4081 ctatgccacc atccgcgaat tggtgctttc gtattttgat gacttcttca atgttcatgg
     4141 gcaaaagacc ctgcgcacct acaccgctcc catcgatctg gcagcctttg atgactggca
     4201 atggatgaca gaagccagcg gggtggacag aatcgaagaa ctcttgaagc gtgccgacct
     4261 gaccgcgctc atcacagaag aacaggcaag atatctctca cctgtggacg agcgttcgtt
     4321 taaagcgggc acgctgggaa tcaatccaaa gggaacctat aaaccccaat aatgccaaga
     4381 cagataacaa gcacagttga caaaacttga caaaagttga cagtagcaaa aaatgcaaaa
     4441 gatttgatcc ttaggatcaa atcgggtaac gcatagatta acggcgatac tgttgtgtat
     4501 gcagaagaag ctggtaaagg ttttaaagtc gatggttcct tgcccttttt cccttacctg
     4561 tgaagagccg aattgtgtgc tatactaacc cattcatcta ttccaaggca gcatcatcat
     4621 ggaccgcaaa agaatcgcaa tcttatctct atcgctactc accatcatct acactgacac
     4681 catagcgccc ctggtacagc aaatgtgcgc cgccttcccc cagatcagcc caacgctggt
     4741 gaagatgacg gtgaccctgc cggcgttgat gaccattttc ttcggtttga ttgcagggca
     4801 gatggtaaag gtgatctcca aaaaagcggt gctcgccatc gggctgacga tgtacagcgt
     4861 gggaggggtg gcagccggct acgtgcacaa cttcaccata cacctggttt accgcggtct
     4921 ggtaggggca ggggtagggt tgatcacccc catcatcact tctctggttg cggactacta
     4981 tcagggcaag gaacgcgcgg atatgatcgg ctacaatttt gccgtttcgc atttcgcagc
     5041 agtgatcacc ccagcgctgg caagctatgt aggcacggtg agctggcgca acgcatttct
     5101 gatctttctg gtggtgccac tggtgtttgt ggtggcgatg atcttgctgc cgggagcttc
     5161 tcgcaataac cagccgggca agataaagga gaaagcgccc atcacgacgc gcgcgatgca
     5221 atgcgctgtg atcgccatgc tgctgatggt gtttttcttc gtggtcgtga ccgacctttc
     5281 ttacctgctc aataccaagg atgtggtgag ccctttcctt gcaacatttg ggctatccac
     5341 attcacgttc ggctttgcca tcgccgggct tgctttttcg tggatctacc tgaaaatcca
     5401 acgatgggtg atcccgagcg gtttcgtggt gtgcggagcg ggttattttc tggtgacgtt
     5461 cagccaaacc aacgcgctga tcttggcagg attgctgagc attggattgg gaatgggtat
     5521 gttgatatcg ctgctggtcc tgcaaaccac caattctgta ggaaaagccg acagcacagc
     5581 ggctgcagcg ctgaccaacg ccggtttctc gaccggcatc ttcctttccc cattctttta
     5641 cgaaaaactg cccagcattc tcacaggtat ggaacggatc gagttcaact tttttctggc
     5701 gggaggaatc ttcttgctgg caggggtgat cacgttgacg ggcttgatcg ttcgacgcaa
     5761 aaagctggca atccaagcat agcattacag aaaagcatat gaagagagcg tgctacacaa
     5821 gcaagtgtgc tccctttatt tacggctccc acacatttct agccggatcg tagctgatat
     5881 catccagcca atcaccgatc accacgccca tcaccagacc ctggttgttc tcatccatcc
     5941 tgacatacag gctaaccggc atgacccccg caccggtctc acgttcggtg gtgacagacg
     6001 cgccacccat gagggaggca gcattgcgct gcgcgatgga attcgtggca gtaatgatcc
     6061 actctttggc aggccaggaa tcgccaaaga tggtactgat gagcaaaatg aggtgttcat
     6121 tctgcgcatt ggcatattcg gcgctggcgt caatcgaaag gtagataagg ctgcgcgcag
     6181 ccagcacatc ctcacccgat accacgacct gcaaagagga catgccatcg ggtgagaccc
     6241 cagtgatgag ttcagacccc ttataagcgc gggactctga aaaagtgaaa gcgggcgcct
     6301 gaaacgcgct cagaatattt tcgagcgtgg catccggaaa agcgctggta agcgggatgg
     6361 aattgatctt agcggaacag ccggcaacca atacaaggca ggcaaacaag caacacaacg
     6421 caatggggtg taagggtgtc tttttcatgg gtcctcttct cagtaaagat atttaacaaa
     6481 cggcttttga ttatgccatc aaatgacgaa agcgtaagcg cgcaatttgt attgcgcata
     6541 gatagaatat tgggagggcg caaagacgga aaaatgccca agtattcaat gctgcatagc
     6601 ctttcactca ctttcacgtt tctggctttt cgaaataagc cggatcatgc aattctcctg
     6661 gggttgaaca agccgcagct ttgattataa tcactgtgtg actatcatga agaatatttt
     6721 tctggcggta acgctatttg tgctaaacag cctgaacgct ttctcccttg ccgatgaggg
     6781 aagccagccc acagcttatg atctgatagc ggaggtgaac gcatatcggg cggccaacgg
     6841 ttattatcag ctcgcaccca attcgctggt gatggcagca gcgcagacgc acgctgaatg
     6901 gattgtggcg accggacaag gcgggcatat cggggcgggc ggcagcgacg agaccatgcg
     6961 cgtctcctgg acaggctacg gcgggggtgc tgaaatccac tgcgatgaaa actgggcgag
     7021 cgggcgcacc gtcagcgacg tggtgtacgg agcctggtct gattggacgc accaggaggt
     7081 catgctcaac gcctggggca atcggtatac ggatgttggc gctggcgtag cggcgcgcgg
     7141 gaatggcagt tacgtgtttg tactgaatgt gtgtatggtg gtggggcagg gatcgagcgg
     7201 atcggtgcca ggtgccacaa gcaacccact tgccacgcag gatctgtcga attacgttta
     7261 tggggtaacg aaagccaccc ccctgacaga tggcacgatc aagcacaagg tgctgtatgg
     7321 tcagacctta acgaccatcg ctgaagctta tggggtgagc attgaaaccc tgcaagcgct
     7381 caacgacatg acagcggacg ataccaccat ttgggtggat caggagctcc tcatccagat
     7441 aggaactgga acagaagagg cgcaggaaag cccgacgccc agcgcggcag cgacaacgcg
     7501 tccgacacag acggcgagcg tctcatccag cacggctacc ccccagagtt ctcgagaaaa
     7561 ttccagagtt gggttatctg ccaaaacact ggggattttg ctggtgggaa tctcagtggt
     7621 ggggctggtg gtggttgtgt atcttttctt catcaagaaa taaccggata ggcactttcc
     7681 tgctgctgac agaaggaatg agccgtcagc gactacatca tccatgggtt ccttggaatt
     7741 gaagcaggtc aggatggaat aagcctgttt gttctccgtt ggaacagacc gtatcaaagc
     7801 ggcacctggt tgatttgcgc aaaactttcc tcgacggcgc tgcgcaacca tggaaacgga
     7861 tagagggcgc gggctgtact gacgggaaag tgatgcgcat agcactgatg ccgtataatt
     7921 gaaacatccg agttagaagg aagggaagac aggcatggct aagtatatag gagtattgac
     7981 cgcagggggg gatacccccg ggttgaacgc cgctctgcgg gcactgggaa aatcagcgct
     8041 gggagaatac aacctgcaat tgatcggatt ctacgacgga ttccgcggtt tgatgaatga
     8101 ccggtcggtc attttggatg aacagaaatt atctggcatt ctcacttttg gaggaaccgt
     8161 tctgggaacc agccgagaca agccgcaccg catgccggtg ggagataaat tgatggatat
     8221 gacggatgcc atgctggagg tgtatcacaa acatcatctg gacgcgctgg tgtgcattgg
     8281 cgggggaggg acacaaaaaa atgcctatcg cctgatgcaa aaaggcatga atgtgatcac
     8341 catccccaag accattgatc gggatatcgc catgacagat gccactattg ggtttgacac
     8401 cgcactgggc attgccaccg aggcggttga ccggctgcac agcactgctc acagccacca
     8461 tcgcattgtg ctggttgaat taatgggaca taacgccggc tggctggcat taggagcggg
     8521 cgtagcaggc ggggcagatg tcattctcat ccccgagatc ccttatgagc ctgccaggat
     8581 tgccgaagca atcttagcgc gggtgcacag cggaaagcgt ttcagcatcg tggctatttc
     8641 tgagggcgcc acctccaaaa aagctgtcgc ggagcttgac gcgctgaccc aagaaaagaa
     8701 gaatgccaaa aataaggaag aaaaaaggaa agttggggaa aagatcgaaa atttcctgca
     8761 ctctcaatcg aacaagacca tgctgctggc tcaagagctg gaaagcctaa ctggacttga
     8821 atcgcgcgtc actattcttg gttatgtgca gagagggggc accccttcgg cgcgcgaccg
     8881 tttactggca accgaattgg ggaccacggc tgcctcgctg ttggcgaagg aaaaatatgg
     8941 atgcatggtg gcggttcacg aagagcgcat tgaagcagtt ccactggaaa aagtggttga
     9001 ccggcgtaag gtggtacccc tggatcattc ctgggtcaag acagccaagc gggtggaaac
     9061 ctgcatggga gatgacattt aaataaagaa gagaggttct cgaatggaac ctctcttcat
     9121 ttcttgccat ggaagcttat tgagcgacgt agtagtggaa gtacacttcg gagaatgact
     9181 cattggtatt gtttttgaga taccagcgcg tggtgtactc gcctacttta tcaggggcga
     9241 tggagggcag gtcgaaacac catttccctc cgggttcgac agtttcaccg atgtagaatt
     9301 tggtagcgct agaccataat tgatagccac tcacatactg caggtaatac ttggtggtcc
     9361 aatccgttgc gccgacattg gaaagacata catggaaggc aaaattttgc gaacgggtta
     9421 acactgtgcc atctatcggg gtctgggtgt tccatcttgc cgcatcgcca taatattggg
     9481 tggttgcggt gggataggcg ggttgattgg agacctgcgc ggtggcggtg ggttgattcg
     9541 ggtcgacagt gggcgtgggg ggcagggtag gcatggatgt ggcagtgggt tggggagtgg
     9601 cgctgggttg agcgatggcg gtttgggtca tgctaaccat gacagtctcc gcaacctgag
     9661 tgaagacaac attcggatcc tgggttggtt cagcgttggc tcctccagtg caggcgctca
     9721 acaaaatgac cgcaaaacca agaacgagaa atccgttgat ctttttcgat tgtttcagca
     9781 ttttatcttt ctccatcaaa aatgaatatg tattatacac gaagaagcaa gcatgcattt
     9841 ttataataca aggcagccta agcataccac gcggtttgaa atgaatcaaa ccagcttatt
     9901 cttcgaacag cgcatatttt ttctgctgtt cctgccggaa ttgctgcgtg taaagatggt
     9961 aatatttgcc ctgtttctgg atgagttcct tgtgggttcc catttcgcta atctgaccgt
    10021 tttcaatgac caggatgcga ccagcgttct tgatagtgga caggcggtgt gcgatgatga
    10081 aagtggtgcg gttgaccatg atggagtcga tgcctttctg cagggcagct tcggtgaggg
    10141 tatcgaccga tgaggtggct tcgtccatga tgaagatttc aggatcagaa agaacagctc
    10201 tggctaggct gatgagctgc ttttgcccga ccgagaggag cacgccccct tcgccaaccg
    10261 gttcatcata gccatgtgcc attttcgtaa tgaaatcatg cgcaccggct aatcgggcag
    10321 ccgcttcgat atcttgatgg gtggcgttca aacgaccata ggcgatattc tccttgatcg
    10381 ttcccgagaa gaggtggggg gtctgcagca cgacgccgat gcgcgattgg atggcgctta
    10441 atgagaaatc cgtgtaatct ttgttgccga tgagaatacg tccccgcttc ggttcgaaga
    10501 agcggcagat caaattgacg atggtggtct ttccacctcc ggttgagccg accagggcga
    10561 cggtctctcc ctgttttact gtgaagctga gctctttaat caccgggcta ccgtcttcat
    10621 accagaaaga gaccttttca aattcgatat ctccgtgaag ggaacctgga tcgaacgctg
    10681 tgggtttatc tttcacatca ggaggggtat caatcaaggt gaagatgcgc tcggcggatg
    10741 cgatggcgcg ctgcatttca gcgatgacgc gtgccagatc ctggatgggc cacatcatga
    10801 aggttatgta ggagaggaaa gcttgcatac caccaatgct aataccaccg acggtagcct
    10861 gagcaccgct ataccagatg accccgccca atgccagcga cgagatcaac tgcacggtgg
    10921 gcaggaacaa cgcactgatc caggcagcct tataagatga tgtatacatc tccccggtga
    10981 ggtcgccgaa ttctttcagg ttctcttttt ctctgcccag cgccttgatg acgcgcacgc
    11041 cggtaatatt ttcgttatac gaagcggtga tctgcgaatt gatcttgcgc acaacacgat
    11101 actggcgcaa gatgcgggtc tggaatttaa tgccgacata cagcagcaaa ggcagcatgg
    11161 cgatgacaat aaacgcgagg cgggtattga tcttgaacat gaagtacatg gaagtggcga
    11221 tattcacgat cccccaggtt gaatccagga taccccaggt gaccaattca gcaacgcgct
    11281 cggtatcgtt ggtaacacgc gacatgatcc aaccgaccgg cgtttgattg tagtaggaaa
    11341 gcgaaagatt ttgcaaatgg ttaaacaatt tttggcgcag gtcgaagcgc accttttcac
    11401 ccaccgtgct gacggtgtag acaaaggtaa atacggtgcc ggcttcaaac agaacaatca
    11461 gcgcatactg gtagagaatg ttcaaaagaa tgccaacctg accagaaagg atgccctcgt
    11521 cgatgatgcg tttggtcaga aaagtagtgt aggcatccat gacggaggtc agaatgaccg
    11581 cgaccagaaa aaccagcatc aatccccaat gaggtttcat gaacttgaag atgcgcaaaa
    11641 agatcttgcc ggtcgttcct tcgcgcgaat aatcgatttc ttcaaatgta ttaactggca
    11701 cggtcgatct ccttctgaag atcctcttcg atcctggttt gaatgtcgaa aatattacgg
    11761 taaacccctg cttgcttgag aagttcctga tgggtgcctt tttgactgat gtgccccttg
    11821 tccaaaatga ggatcaagtc agcgttcatg atactttgga cgcgatgggc gatgatgaaa
    11881 gtggtccgat tttgcatgag tttttggatg gcggattgga tatgcgcttc ggtctccgca
    11941 tccacagagg aggtcgagtc gtccaaaata agaatacggg ggtccttgat gaaagtgcgc
    12001 gcaatggcaa tcctctgctt ttgcccacca gacagggtga caccccgttc cccaaccagc
    12061 gttttataac cctgggggaa ggaaaggatg acatcgtgga tggctgccat tttggcggct
    12121 tcttcgacct gtagctgggt taccttttct ttgatcccga aggtgatatt ttcataaata
    12181 gagcgggaaa agaggaaggg ctcctgttcc acaatgccaa tctgttcgcg cagaaaacgg
    12241 cgagtgtaca aacgcaggtc actgccatcc aacaaaattg ccccttcggt gacgtcatag
    12301 aatctgggca gcaaattcac cagactggtt ttcccagagc cggtggaacc aagcagggcg
    12361 acagccatgc cagctttgca ttcaaacgta atgtcatgca gaacctcgct gccgtcttca
    12421 tagtggaatg aaacgttcaa gaaagaaata tcacccttga tcctggtatt cgacgtgatg
    12481 ctgccctgat cgagaggttc gcggtcttcc ttgataatct tgtacaggcg gtcatatgaa
    12541 accaagccat tggacatctg aacgatcatc ctgcccaggt tgcgcaacgg ataaatgatc
    12601 cacaccacca gacccgcgta tgccatgtag gttccaaccg tgatgacgcc atccatagcc
    12661 attttggcgc caatgaaata tccgaacaat agctgggcac cgcaaaaaat atcagagatg
    12721 ggccaaaaaa gcgaatggag cgccatcaat tttctaccgc ggttgtattt ttcgaaattg
    12781 tcgcgtttga acttctcgat ctcatagggt tggcgcgcaa acgctttgac aacccgcaca
    12841 ccgctcaagt tttcctgcaa agtggtggaa agaatggcat cctgttcctg gtacgcttcg
    12901 tacgctttgg tgactttttg gaagaaatag atcgaaatga aaagaacgat gggcacaaca
    12961 atgatggagt acaaggcaag tttgccgttc aacctgagca aagcgatgaa attcaccaga
    13021 aaaaggatga tgattctgcc cacgccgata gcctgatcgg catagaagcg gcggatagca
    13081 tccacatcgg aggtgcagcg ctggacgaga tctcccgtgt ccgttttggc atgataagcg
    13141 aaggtgaggt tttggatgtg atcatacaaa taatccctca agcggcgggt ggttccttct
    13201 gcgacctgag cagcatcctt ttggctaaaa aaggtagcta atccctggaa gaggaaaagg
    13261 aggataaacc caatggcaac agctaccacg cttaccgttg cgctttgagc gagcaggaag
    13321 ttatctacaa aatattccaa cagcaaatac gttcctgatt ttgccagggc agctatgatc
    13381 tggaagatca tggctccgat atactgtatc tggtatccac tcatgagaag ccataagccg
    13441 ctcaggcgtt tttcgttcaa aatcttctta aaatcgaggg tttgatattt cactgtcatg
    13501 aattcatttc cttgggttgg ttctgaagga tccgcttgaa ttatacggca tgttttgcca
    13561 gcacattgct tttttatcaa aagctgtact agaataacag gaatttatcc aataaggaat
    13621 agccggttga agtactttaa agagattgaa acaggcacgc ttatctaaag cccgatgttt
    13681 tctggtgata ctactgactt gtttcagaat acaaggacaa cgagatgggc tatgattagg
    13741 atgaaagaag ggagcggaat cgtggtggca aacaaaaaaa tacggatgat gatcccggga
    13801 ccggtggagg tgcacccgga tgttctgaaa gccatgggat cagcggttga gccgcactat
    13861 ggggacgcat gggtggcgaa gcagcaacgc gtaacggaaa ttttgaaaga aatcttcaac
    13921 actgcaggag atgtgtacct gttggttggg tcaggcacct gcgcgattga cgcggcaatt
    13981 ggaagctgtt tgcagggggg cgaaaagata ctggtaggga cgaacggcta ctttggcgac
    14041 cgcctggttt ccatcgccaa acataatgga ctggaagtga tcgaggtcaa ggcgacgtgg
    14101 ggagaagagc ttgacgcgga tgaaatcgag agggcactgg agagagatcc atccatcaaa
    14161 gcggttgcgg tggtgcacag cgagacttcc accaccgttc tcaatccaat tcaagatatc
    14221 ggaccaatcg tccgaaggca tgggcaactc ttcatcgtag atgcagtatc ttcgttgggt
    14281 ggcgttccct tttctatgga tgaatggtgc gttgacgcct gtgcaacggc tacgcaaaaa
    14341 tgcctgggag cgcctccggg gttaggaccg attgcagtca acgagcgcgc atggaagagc
    14401 atcgacagat tgcaaacagg cgagcatggc tggtacactg atctgcgggt gtggcggcag
    14461 tacgcgcagg aatggggtga ctggcatccg acaccggtca ccatggcaac caacaatgtc
    14521 aacgcgctgc tggtatcgct gacgcaattg aaagcggagg gtatcacaaa gcgcatggag
    14581 cggtatcgca gcctggcgct gcaactgcgg gaggggatgc gaaaagcggg aatcgcaccg
    14641 ttcactccgg atgaaaagtt gaatccggtg ttaactgccg gggttgcgcc gagcggagtt
    14701 aacagcgctg agatcgtccg gtattttcta caggaatatg ccatccaaat ctcaggaggg
    14761 ttgggagccc tgaaagcggg catctttcgc atcgggcata tgtcgccggt catgaccagc
    14821 gaggatatcg agctggtatg tgaggcgctg gcgtctttct gccgaacgcg caatctgtga
    14881 gaaaatgaac cagaaccggt gacgaacgcc ggttcttttt ttcaaaataa gtctataatt
    14941 aagccactat attcgttccg aaaggaggaa actatggcga acgttatctt tgaccatgta
    15001 gtcaaaaaat tcaacgacgt cgtcgcagtg aacgatctca gttttgtgct ggatgataag
    15061 gaattcctgg ttttggtggg tccttcggga tgcggcaaaa ccaccgcctt gcgctgtttg
    15121 gcgggactgg aagaagtgac ctcgggggaa atcaaaatcg acggaaaagt ggtgaatgat
    15181 gttccaccga aagaccgtga catcgcgatg gtattccaat cctacgcgct ctatccccac
    15241 atgacggtgt tcgacaacat ggcttttgga ttgaaactgc gcaaagtccc caaagacgaa
    15301 atcaaacgca aggtggaaga agctgcaaaa gtgctgaaca ttgaaactct gttaaagcga
    15361 aaacccaaag agctctcggg tggtcagagg caacgcgtag ccgtgggacg cgcgattgtg
    15421 cgcgagccca aagttttcct ctttgatgaa ccgctctcta acctggacgc aaaactgcgc
    15481 gtgcagatga gaaccgaaat caccaagctg caccagcgct tgcagaccac tttcatctac
    15541 gttacgcacg accagatgga agccatgacc atgggaacgc gcatcgcggt gttgaatcac
    15601 ggcattttgc agcagatcga taccccgcag atgctgtatg acaaacccaa caacctgttt
    15661 gtagcgggtt ttatcgggtc tccttccatg aacttcttca acggcaagat tgagaagaag
    15721 gatggcaagg taatgctgaa agcggagagc tttgaggtag agatccccaa agaaaagata
    15781 aaggtctacg aacatctggc gggaaagaaa attatctttg gaattcgccc agaggatgtc
    15841 cacaatccgg aatacttgcc ggcacggatc aaacctgcga tcatcaagag catcgtggat
    15901 gtgactgaac tgatggggaa tgagatattc ctgcacttga agccatctga aaaagatgca
    15961 gtatatgtgg cgcgagtgga tcctcgcacg cgtgtgaagg tcggggacgt gatcgaggtg
    16021 gctttcaaca tggaaaacat ccatgtcttc gatccttcga aagacgcgga aaatcctgtg
    16081 gctgttcgat aaagcctgat ctacgcgata aaaactgcac ccttgctgtt ggttgcgtcc
    16141 aacttgtggg gtgcagtttt tttctttcca tactttttcg atcatcgttc aagcggcaaa
    16201 ctgccgtttc gaataccgtt aatacctgca agaaagccag gctctggcta ttttctttct
    16261 gccgttcctg ccagatgcgc tactcaaccc tttctttttt cttgagcaga ctaccacagg
    16321 cataatgcaa gaagaacgcc agggtgaagc tgatgatgcc aaccgtgatg aagacattta
    16381 cccagaaggg ggtggggaac aagcggatca gggagtcgct tgggtagaag agccaggtat
    16441 ctccttcgaa gaagagggaa tgaaagatgg tgaaaagacg gtcaaaattg agaagtacag
    16501 ccaaaagtac aaaaacgata aaaaagaaga tgattttgct tgccagtttt ccggcttcga
    16561 gtaagtgggt ctgccaatgt aatttcaatg ccagcactat ccccgctgcc atgacggcga
    16621 tgctggcata ccagacagct aatacgatgt gggtgaggtc acgcacgtca cgcatatggc
    16681 tgagctctct ttggttgaac agcggcttgc catcaggcgt cgcgagcgcc tgataatcgg
    16741 catctgataa tttcccaagc agataatcca ccgattgata agccaggaca gtccgggtat
    16801 ctctggaaaa accgtaggta tcgtcaggga aagcggggta agcgtattcc aggtcaatga
    16861 aggtatgggt caggcaaaag cgtatccccg cgatcaggat caccaaaatg aaagcaagga
    16921 acatgatatg tctatacaca gttttcatcg caacgtttca acgcctccgg cacggtaaaa
    16981 atcgatcagc atggtattga ccaaattctc caccggtgca cgatcatccg taaaaacgag
    17041 gtcgcttgcc cctagaggct gcaggttcaa aatggcggtt tccatggatt ctcttaacaa
    17101 tgcaggaaaa tttcctgacg catcgaggaa caaataatta ttatacaggt tctggacgct
    17161 tgtagcggag cgggtggcat acagcatggt gttgaacgtg tctggaacat cgctgatata
    17221 gacggacgga aaaacgcgac cgagggtggt ggcgagcgca ttcaccagac ggcggtcatc
    17281 cccgatcctg gcgacgttca aaaccagggt gccatctgcg gagaggtggt cgtatacctc
    17341 ctgaaagaat tccaaagtgg tgagatgcgc cggaatatag gggggacggt aagcatcgat
    17401 ggaaatcagt tggtattcct tgtgcatcgc gcgcaagccc aggcgcgcat cttgcgcgaa
    17461 aacggtcaat tggggcacat ccattccgaa atagtcatat ccgacttcaa tcacggttcc
    17521 atcaatttca aagccgtcga tatgaacatc aggaaagacg ctgacggctt gcctggcgct
    17581 ggttccccct gctaaaccca agattgccat ggagcggaga tcttggggtg aataaggggc
    17641 atcctgaaaa aaaggagcca ccaagacctg ctgccaggtt cctccatagg cgagggtacc
    17701 aggatgatag atggagtgaa tgccctgccc ttcattcaag cggagcaggt tgtagccatt
    17761 gatctgctgg acttgaatgt agttgtattc tgactctttt tcgtagatca aaccggtgga
    17821 gatcttatcg gtaccctgca agccaaacca ggcgctgagg gcgatgacca gaggcatcca
    17881 gccaaatttg atgagggttt ttctaaatcc aagaggggtg gtgcatgaac cccaaaaacc
    17941 gaccagccat aaaaaagagg caaggatgag aaaattcttg tacgtgccaa cggttgggat
    18001 caaaacgagg acgggcaaaa aggttccaat gaacgaaccg gcggtggata tggcgtaaac
    18061 tttgcctgaa atgttgcccg aagtgcgcgc ctcatccacc aacaaacgga tggcaaaagg
    18121 tgaggtcatg ctcaacaaga ccatgggaac gctgaagagg gctaataccg cagcgaaagc
    18181 cagaatgagg ggaccaatcg acaaggtatc catggcgaga ctggcagaac gcaagaccgg
    18241 tttggcgata gaggggatca agccggtgct aaaagcgccc caggtgatga tgcggtagaa
    18301 cagctcgagc tgtggggagc ggtctgccca ttttcccccg aggatggagc cgatggtcat
    18361 atagatcagg atggagccga tgacgcatgc ccagatcagg ttgctcgatc cgaaataatt
    18421 ggataacagg cgggaggcgg atacttcgat agccaaactg accaaccctg ctccgaagat
    18481 cgcgaagtaa atcctggtac gcatgggatg attatatcga aaaagaagga tgaatatcaa
    18541 atataatgaa agagatcaaa gcggaggttt gtgtgtatca gatcgtaagt gtggaacaga
    18601 tgcgggctct cgaacatgaa gcggacagcc gggggttgag ctacgcgcag atgatggaaa
    18661 acgcggggaa ggcagccgca accttgatca atcagcggtt tggcaagaga aaaggcaaga
    18721 gcatcgtcgg tctggtgggc tcaggaaaca acgggggaga cgcgctggta gctttaaacc
    18781 agttgcagtg tcaaggttgg gacacccacg cttatctcat caaagagcgc aaggaacacg
    18841 atcttctggt cgaacagtac ctggctggcg gcggcaacgt agaccgtttt tcaaacgatg
    18901 aaaaccattc tttgctcgat aacctgacca agcactgcca ttttctgctg gatggcatat
    18961 tcggaacggg agtacgcctg ccactgcgag gggaaattga agaggttctt cagcacctgc
    19021 gcgacgctga aaagctgccg tatatcattg ccctggattg tccatcgggt gtggattgtg
    19081 attcaggaga ggctgacgtt gctgccgtgc acgcgcaatg gacgatcagc atggctgccg
    19141 tgaagcgggg gctactggaa ttcccggcat ttgagcacgt tggcgaactg agcgtggtgg
    19201 aaatcggtct gccggcagat ctggaagcgt ggaaaggggg gagggagttt tgcatggatg
    19261 aaagcgctgc ggcggcaatg ctgccagcgc gcccagtgca ggcgcacaaa ggcacgtttg
    19321 gcaccgccgt gatctttgcc ggttcgcgcg cctacccggg agcagcttat ctggcggcaa
    19381 aagccgccta tttggcgggg gcggggctgg taaccctggc aagcattgcg gaggtgcggc
    19441 aagccatcgc cggaacattg cctgaagcca cctggtattt gctgcaaacg caggatgagg
    19501 gatttgggtg ggacgaaacc acctcactgc cggctgtact ccaaaaggct tctgccattc
    19561 ttgctggacc aggctgggga acgcacccaa gtgcggcaat cttgctggag cgcctgttcg
    19621 atataatgaa agagaaccca cgcaccccgc tcattattga tgcggacggg ttgaacctgt
    19681 taagtcagca tgtggaactg tttaaaaaac tgccggcaaa ctgcatcctg acccctcacc
    19741 cgggcgagat ggcacgcttg agcggacttg cggtagaaga gatacaaaaa gacaggcaaa
    19801 cgatcgcaag aacgtacgcc aagaaatgga acgcgaccct ggttctcaag ggagcgttaa
    19861 ccgtgattgc cgacccctgc ggcagactgg tggtgaaccc cgtggcgacg ccagccctgg
    19921 cgaaagccgg aagcggagac gtgctggcag ggatgctggc aggcttggcg gcgcagggaa
    19981 ttgatgggtt ggacgcggct tgcatggcgt gctggatgca cggacgagca ggcatgctcg
    20041 ctgaacgcgc tgttgggcat tctgccagtg tgatggcgag cgacatcctg cgatctatcc
    20101 catgggtatt tcgcatactc ttaaaaagaa aagcagggtg acgattgagg caccctgcca
    20161 acttttcctt ccgccggact attccgattt ggcgatcttt tttgtggaag gagttttctc
    20221 tttcttgctt tcgagaatca cctgcccctt ttcagcgacc tctgaggcgc tctgcatggt
    20281 atcgtcgatg gcggcggata catcaccaac cgttttttcg gcttgcgtca ggacctcttc
    20341 ggcatagttg gcgactttgt cacgcaattc gatcgatttc tctttaacca atttgcgcgt
    20401 ttcttctcct gcctgaggag ccaataagag cgcaaccact gcgccggcaa ttccacctaa
    20461 gagcaaaccg gagaaaaatg atccaaaatt atcattatca gacattgatc ttacctcctg
    20521 aagagaatta ttttatttct tcaatattcc tagaatttca aacagttttt ttgcaccagc
    20581 catatagctg ttggctgcga tcaccggctt tacggcgcgc ttgctgagga aatcggctgt
    20641 gcctttcacc gtgtcgatgg tttctctggt attggagatg atgggtttga tctcattttg
    20701 cagcatattg gtaagcgcag ccagttggat gaccaaaata accagagcca ccccaatgat
    20761 aaacgattca aacgccagga caataatgaa aatgtcgcgc acctggctgg tggcatcagg
    20821 acctgccttg accagggagt aaatcccgta gatactggca gccatgacca acaaaaaaat
    20881 gatgatgccc cacaccaatt ttctcttatc gatcatattg gcatttggat cggcaggcgg
    20941 ggtacctggt aggggttgtg acgattcgct cattttgttc atctttttct gtgttttgat
    21001 tataacatac ccgttttttg ccttttttcg gaagggaaac aggcaagcta tacatggtgt
    21061 gggtttcaaa aacccacttg ctacaatcac ggatccatgc aatgattcta ttaaagtaac
    21121 aatggaatag aaaaaaagat acgccaccgc cagctacgag ccgcatttgg aaaatcatca
    21181 aatctgtatt aaactagtaa gttacttcat tgtctatttt aagggaaaaa catgacaggt
    21241 gaaaagcatt tgatcgtcgg tctggggaat ccgggtaacc aacacgagaa gaaccgccat
    21301 aacattggct ttatggttgt tgatgcgttg gcgaagcgct ggggaatcag catgaagcgc
    21361 aaccgaaaga atgccttaac cggcgaaggt acctatcagg ggatcccggt tgtgttggct
    21421 aaaccacaaa cctatatgaa caactccgga ctttcggtag gtcccttgag ccggttgtac
    21481 gaaattctcc cggagaatct gctggtgatc tatgatgacc tggacctggc atttggtcgc
    21541 attcggctgc gcgcatcagg cagcgcggga gggcatcacg ggatggaatc gatcattgaa
    21601 aaagcatcca ccagtgaatt tccccgtctg cgcataggta ttgggcgacc ccagcatgaa
    21661 ggtgaagatg tgattgattt cgtgttaacc ggttttcacg gacctgacaa acagaaactg
    21721 aaaaccgttc tcagccaggc agcggacgcg gtggaaacct acctgcaatc gggcatcgag
    21781 atagccatga cacagttcaa tggcgtcttc ctggatgagg aatgatcaat atccgttttc
    21841 acgaattgga tgggtttact cacgcactca gcgcggtgcg cgggggacga cctgagggtg
    21901 ccttgggtct ggtgaaaccg gtgcgcctgc ctctggcgat gacgttgcaa aaagaactca
    21961 aggtaccttt gctctatgtt accggacaga tgaaccgcgc acaggttctg atggaagaag
    22021 cgcaattctg gaagcagaac gagaatgcgg aactgtacgc agaaccggtg ccactcttct
    22081 atgaagatag cgattgggga gcaaacgttc gttctaaccg gatcgcggtt ttggaaaagc
    22141 tctcacgcgc ctgggtaccc ggcaaagagc agaacgcaga agacgtgttg atcatcgctc
    22201 cgataaaagc gctgatgacg cgcaccattc cacgccgcga ttatctcatg gcaagaaaag
    22261 agatcaaggt gggtgcggag catgacctgg aaaaactgac aaaacacatc ctgcggttgg
    22321 gatacgagtt tcaagaggtc gtgagcgggg tggggcagtt ctctcggcga ggtgggatcc
    22381 tggatgtctg gctggctgac caggcgcacc cactgcgtat cgagtttttc ggtgatgagg
    22441 tggattcgat gcgggtcgtt gacgaaacca gccagcgctc gatggaacaa ttgaatgcct
    22501 gcgtgatctt gccggcgcgg gagttcatcc tgccggaaga cacgggcgaa gatatccgac
    22561 actaccatga acgggatatc tgcgtgctgc atcccaacgc caccagcctg ttttcttacc
    22621 tgccaaaagg cagcctggtc tgcctggatg accaggacat tctgatcacc gtttcagaag
    22681 aggtggaaaa ggatgccctg gagctgagag aacagttgat caaagataag agactggaaa
    22741 agaacttccc ggcgccttac gattcctggt cagaactcta tgaccagatg ctttcgttca
    22801 atcccctgaa catgagtttc tcaggggtgg aaaccctgca acttttcagg caatgcatcc
    22861 aacctgcgcc gcgttttggc gggaagatgg atgcgctgct gcaatttcta gaagaagagg
    22921 ttgaggaaaa tcacagtgca acgatggtca gccgccagag cgaacgcttg caaaaaatgt
    22981 ggcaggagag ggcagccggc aaggaaaagc aggtgacctt tctcaaccaa tatcttagtg
    23041 cgggctggaa ggtcatctat cctgatggaa ccgtggaata ctgcatcacg gatgaagaga
    23101 ttttcggctg gcagccggct attcgcgcga aacgccgcag cagccaacaa ctgaccgtct
    23161 ttgacttcgg agccttcgtt cccggcgact acgtggtgca tttcgaatat ggcattgcgc
    23221 gctaccaggg attggtgatg cgcaccctgg aaggggtgga gaaagaatat ctgttgctgg
    23281 aatttgcgga gaacagtgaa ctgtacgtgc cagtacatca ggcagatcgc atttctttgt
    23341 atatcggtcc cgacgctcga ccacccaaac tatcagcgtt gggcagccct aactgggaag
    23401 gcatcaaaaa ccgcatcaag caggcggtgc actatgtggc gggcgacctg ctggagcttt
    23461 actccaagcg ccaatcggta gaagggtacg cttttcatga agacaccgat tggcagcggg
    23521 aactggaagc cagttttccg tatgaagaaa cagaagacca actgcaagcg attgcggatg
    23581 tgaaaaggga tatggagcgc gcaaagccga tggaccggct gctgtgcggt gatgttggtt
    23641 atgggaaaac cgaggttgcg gtgagggcgg cattcaaggc tgccttggat ggaaaacagg
    23701 cggcgctgtt ggtgccgacg accgtactgg cgcaacagca cctggatacc ttcagacaac
    23761 ggctggcacc ttttcctgta caggtggaaa tgctttcccg cttccgcacg gaagcagaac
    23821 aggaagagat tctgctggcg ctggaggcgg gcgagatcga catcatcatt ggcacccacc
    23881 gtttgctgca gaaggatgtg gcattcaaag acctgggttt gctgatcatt gatgaggaac
    23941 agcgctttgg agtgatgcac aaggagttct tcaaaaagat gcgcgagaag atcgacgtgc
    24001 ttaccctgac tgcgacgccc attccgcgaa cattgtacat ggcactgtct ggtatccgtg
    24061 atatttcgat gatcaacacc gctcccaccg accgtttgcc agtgaccacg catgtcggtg
    24121 gatatgattc agaattggtg cggcgggcga ttctgcgcga gatcgaccgg ggcgggcaag
    24181 tcttcttcgt gcacaaccgc gtgcaaacca tcagcacgtt cgctgagcag ttaagggcgg
    24241 tagtgcctga agcgcgcatt gcggtggcac acggtcagat gcccgaacag caactggcaa
    24301 gcgtgatgta ccaatttacg gaacaaaaag tggatgtgct gctatccaca tccatcattg
    24361 aatcgggatt ggatatcccc aatgccaaca cgctgatcgt ggaccggggt gacacttttg
    24421 gattggcaca gctttaccaa ctgcgcggaa gggtggggcg cggcacacaa aaagcgtatg
    24481 cctatttctt tttcaattca agagggaacc ctacggttga aggattggag cgtttggagg
    24541 tgatagcgga gaattcgcag cttggttccg gctataccat tgccatgcga gacctcgaaa
    24601 tgcgcggagc gggagatctg ctaggaacac aacaacacgg gcatatcgcc gcggtgggct
    24661 ttcatcttta cacccggttg ctggcggaag cggtaaagaa catcaaacgg aagcagggag
    24721 aggaacctct ggcggaaagg ttgaaggaag tgctggcaga accggaattg cgaccttttg
    24781 tgagcgttga tctgccgctg cccacatcca tcccggtgga ttacatcccg gatgctgggg
    24841 tacgattgga actgtatcgc aaactggcgc gcgtcaccag tgaaaaaggg tttgaagaga
    24901 ttggagagga gttccgtgat cgttttggag cctcgcctca ggatgtttca aacctgatct
    24961 ggcagagcaa gataaaattg atggcagaga aggcaggttt atattccatt gcagtggaag
    25021 cacaacaatt ggtgatgcgc tatccaccgc tacccaagaa tatcaaacgc cggagcctgg
    25081 tccctctgga accgtactgg cgcagcggga agaatgaata ctggctgtgt atagacatga
    25141 aagggaagga atggatgagc cagcttgaag acgccttgga gaacctgatc gagaaaggca
    25201 agcaataaca ggaggaagca gcgtggattt ccatttgaaa gcaccctatc aacccatggg
    25261 agatcaaccg gaggcgatca ggcaactggt ggaagggctg caacgaagag aaaaacatca
    25321 ggtgttgctg ggtgctaccg gcactggtaa aaccttcacc atcgccaacg ttatccagca
    25381 ggtgcagatg ccggccctgg tgttggtgca caacaagacg ctagctgcac aactgtatgc
    25441 ggagttcaag gagttctttc cggaaaacgc cgtggaatat tttgtatcgt attacgatta
    25501 ttaccaacct gaggcatacg tgccgcgcca ggacctgtat attgaaaaag agaccgagat
    25561 caacgaggag atcgagcggt tgcgcctggc ggcgaccacc gcgctggtct cgcgtaagga
    25621 tgtgatcatc gtggcttcgg tatcggctat ctatggattg ggaagccccg aagcgtaccg
    25681 caacatcgtg atccgattgc aggtgggcga gatcagccgc cgtaacgcat tgctgcgcag
    25741 actggtcgag cagcaataca cccgcaatga tatcgacctg catccgggcg tatttcgcct
    25801 gcgtggggac acgctggaga tctacccggc gtatgaaaaa aagaaagtga tccgcatcac
    25861 cttctttggg gatgaagtgg aaaagatcac catgctcaat ccggtgactg gcgagatctt
    25921 ggaagatctg gagtcggtcg aaatctaccc tgccaagcac ttcatcacgg aagatgatct
    25981 gctcaagaaa gcgatcgtgg atattgaaac ggagctggag gaacaggcga catacctgga
    26041 aaagcataac aaattactgg aagtccagcg cctgcgccag cgcacgaact ttgacctgga
    26101 aatgctgcgc gagattgggt attgctcggg tattgaaaac tattcgcgcc atctcgacca
    26161 gcgcccggca ggttcagccc cctggacttt gatggatttc cttccagccg attacctgtt
    26221 gatcatcgac gaatcgcaca tgaccatccc tcaggtcagg ggaatgttca acggagaccg
    26281 ttccagaaaa ggaaccctgg tggaatatgg gttccgtttg ccctccgcgc tggacaatcg
    26341 cccgttaacc ttcgatgaat tcgaagagcg catggggtac accatttaca cctctgccac
    26401 cccaggaccc tacgagatgg gaaaggtgaa gcacgtgatc gatcagatca tccgtccgac
    26461 cgggctggtg gacccggaag tggaggtgag accggtgatg gggcaggtgg atgacctggt
    26521 gcacgagatc aaactgcgcg tggagcaggg ggagcgggct ttggtaacca ccttgaccaa
    26581 acgcatggcg gaagaccttt ctttgtacct gggcgaactg ggaatgaaag tgaactatct
    26641 gcattcggag gtggaaacgt tggaacgggt ggggatcttg cgcgatttgc gcatgggcgt
    26701 attcgatgtg gtggtgggca tcaacctgct gcgcgaaggg ctcgacctgc cggaagtatc
    26761 actggttgcc attctggatg ccgataagca gggtttttta cgcagtgaaa ccgcacttgt
    26821 tcaaactatc gggcgggcag cccgccacgt gcatggcaaa gtgatcatgt acgctgacag
    26881 cgtcacggat gccatgaagg cggcaatcga tgaaaccaac cgccggcgcg ccaagcagga
    26941 gaggtacaac aaggaacaca acatccagcc ggtctcgatc attaaggaga tctacgatat
    27001 caccgaacgg ctgtcagacc agatggtcac cgacaccagc ggcgggtatg acgcggaatc
    27061 ggggaacctg gtgcacatac ccaaagacgc gctgcagcag ttaatccagg aaacagagca
    27121 gaagatgcag gcggcggcga aggaactgga ttttgaacgc gcggcggtgc tacgcgacca
    27181 ggtatatgaa ctaaaacgca tcctggtaga ggaatcgaat gtagcccctt ggaagaaagc
    27241 catgctgttg gctggagaag atgaggagga aaaggattag agcgtttcgc gcggctcgaa
    27301 ggcagtaaac tgcgcttgat agggcagcca ctcgaccttc acatccgcaa cagaagcgga
    27361 agcgggtcct tgtttgagat cgagcagcat ggcttccaaa gcttctctac gcccttcagc
    27421 caccacctcc acatcgccgt cgaggcggtt gcgcacccaa ccggtcactt cgtgtgtcca
    27481 ggcggattgc atggtgaaga aacgaaagcc aacaccctga acgcgccctg agacaacgac
    27541 gtgcatgcgt gcaacctgcg aggaatccat gtgcgctcac tcctccacat caaacaaatc
    27601 gaagccttcg gaatcttcgg tgaaatcgag attttcgaga gccaaatcaa gcacatccat
    27661 gaggtcttca tccaccgctt cttgcagttc ctgttccaac cgttcgcgca cgcccttgcc
    27721 accgattttg gagagagccc agtagatgtg gtagagcagt tcgttatccg catcttcttc
    27781 atccagaagt tcaaagagga acaggttggc agccgataac tccagttcgc cggctgcgcg
    27841 gatggcttct atttggacgg aggaatcggg atggttgatg tgctcgagca cggtcttttc
    27901 ccagcgctca tcgagggagc gccccatggc gaagagcgcg ctggcggtcc actgtggatc
    27961 attgcgctgc atggcttggg cgatcagccc ggggatgctt tcgttacagg aatagcccag
    28021 tgattccaac gctttgcgct ggatctctcc ggctggtttg cgctgaacga cctccagcag
    28081 tttacccacc accggagcgg ctttttcgcc gggtatctct tccagttcgc ccaataagat
    28141 gaatttgccc aaggctgaag ccgccgacgc acgcaccagc tcagccggat cattttcaag
    28201 aagatgcatg aaatcaggga tgaaagcggg atcttcacaa tcccagagca ggtgaatagc
    28261 ctggctgcgc acgcggggtt catcatcgcg catggcaaat tttgccaggg catcacagga
    28321 aatgagcgtg tcggcttcca tgaggtcttc cagatcttcg agcagggaaa gcttgcgctt
    28381 cagtggaaca gaagaccaga tctgctccag cttcagcaaa gcggaatcat ccaggtcgga
    28441 aaaagcgcgc aggcgcttgg gtgaaaaagg ttgctcctcg ctcaccagtt cggtcaatat
    28501 ttgctcaaaa ggcgtatctc gattttcttc ggtcatcgcg gcagcacgat tgggttcgtg
    28561 aaatcttgga taaacacgaa taccatcact atgattaata ttgtaaaccc aattaaattg
    28621 accgcgcctt caaatttttc aggaacgcgt ttgccagtga tcaattcggg cagaaggaac
    28681 aggatgcgcc cgccatccag ggcggggata ggcagcaggt tgctgtagcc taaagatacc
    28741 gaaaccactc cgaagaacca taggatgttc agcaccctcg atttttgatc cgcaccgctg
    28801 gcttcttcct cttcgcgcac ctgagagtag atatcgtaga tgcctttggg gctgaccggg
    28861 cgcacatccg cgatctctat ttcaccgctt atgagtttcg agggaatttt gagcagcagc
    28921 tcgccctgct cctttgccat gcgaaatgca gacgaaaggc tttcgaagaa cccgatcggt
    28981 tcgaccgggt aggcgaggac gatgcccagc ggtccctggt cagccggatg ctccgcgcga
    29041 ggcgtggcga gcagggtgag ctgttcacca gcgcgttcta ccacaaccgc aatctgtttg
    29101 cccaggttct cagagacggt ggcggtgagg aggtctatgg aatcaatcgt gacatcgttg
    29161 accataagaa tgcggtcatc taccatgaaa cccgcaattt cggctggcgt tccgggctcc
    29221 acctgttgga tgagcacctg atcggtgatg gctttgccgg tgttggagaa gacaatggtg
    29281 aaaagtacaa tgccgatcag cagattcgtg agcgcaccag ccagcagcac acccagacgc
    29341 ttccatttat tctgttcata
//
LOCUS       DAOSDS010000019        28591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_776_length_28591_cov_4.795802, whole genome shotgun sequence.
ACCESSION   DAOSDS010000019 DAOSDS010000000
VERSION     DAOSDS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 28591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28591
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_776_length_28591_cov_4.795802"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLL88_03890"
     CDS             <1..794
                     /locus_tag="PLL88_03890"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ00735.1"
                     /translation="YAVDLFEEKNARSLIDEHKPDVLVNCAALADVDACENDHVRARR
                     LNAELPGWLAEECERHAVKLLHISTDAVFDGRKEGKYREGDEEHPLGIYAQTKLAGEN
                     NVLQVNSDALVARVNFYGFSISKKRSLAEFFLHSLQEGRAVHGFTDVLFSPLYVVDLA
                     QILLEMIEKQLGGLYHVVSPESQSKYAFGVSIAHRFGFEENLIQPITVAEATFLSAPR
                     SKNLMLDPTKTQNALNRTLPGQLEGLEHFYGDYTGGLAERIYALQ"
     gene            822..1889
                     /locus_tag="PLL88_03895"
     CDS             822..1889
                     /locus_tag="PLL88_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOJ00736.1"
                     /translation="MILMIGNHKIGIEHPTYFIADIAANHDGDLERAKSLIRLAKEAG
                     ADAAKFQNFQAHKIVSDYGFKTMGGQQSHQAKWKKSVFEVYRDASVPFDWTPILKEEC
                     DKQGIDYFSSPYDFEAVDMLENYVPAYKVGSGEIDWIEALEYMASKGKPMVIATGAAT
                     IGEVQKAVHAILHINPNLVLMQCNTNYTASLENFKYINLNVLKTYAVMFPQVVLGLSD
                     HTPGHATVLGAVTLGARVIEKHFTDDNDREGPDHLFAMNPETWAEMVENTRRLEQALG
                     SADKFVTENETETVVIQRRCLRAARDISKGEVLTRSMIDVLRPATPGAILPDQLPKVL
                     GLRAVENIPAGREICWIDLGK"
     gene            1903..3003
                     /locus_tag="PLL88_03900"
     CDS             1903..3003
                     /locus_tag="PLL88_03900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ00737.1"
                     /translation="MRVLYCSQDYSPHDHRFLSALSNTEHQIYWLRLENRRRNLEVRP
                     LPESVHQVTWRTGDEKVHWWQYLGLAGRFRKVLNAIQPDLVHAGPIQPVSVLPAMVGF
                     HPLVSMSWGFDILQDATRNSFWKWVTGFVLHRTDRLFCDCQAVKKKVVGWGFPERKIT
                     VFPWGVDLTVFKPDVSKKKIGEAIIFLSTRSWEPRYGLDVALKGFASAVREKPNLRLV
                     MLSDGSQAGWIHNFIRENQLEQHVELRGQIGNDSLSDIYHQAHIYLSASHVDGSSVAL
                     MEALACGTPALVSDIPSNLEWVKDGAEGWVFKDGDTADLARVMVHIASHPELIHEASL
                     MARKKAQQNADWNKNVALLLDGYKRAMKEVVE"
     gene            3000..3788
                     /locus_tag="PLL88_03905"
     CDS             3000..3788
                     /locus_tag="PLL88_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HOJ00738.1"
                     /translation="MKEPRVVAIIQARMGSNRLPGKVLRDIHGKPMLAWVVERARLAA
                     TLAEVVVATTTDAGDDILAQVCKEMGVTCFRGSTFDVLDRYYQAARESEADVIVRLTA
                     DCPLIDPTLIDLVVTRFFEEKVDFATNRLPPPYQRTYPIGLDVEVVSFSALEKAWKEA
                     LEVHEREHVMPYFYDAPGRFKILIVDHETNLGKYRWTVDTQPDLRFVQEVIARLPDKE
                     HFSWLDVLKIVEEHPQLSEINAGIAHKTYLDVDGHATNTGENHA"
     gene            3781..4710
                     /locus_tag="PLL88_03910"
     CDS             3781..4710
                     /locus_tag="PLL88_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00739.1"
                     /translation="MPDPLVTFFTAPKPFTNPHINIIQRNAIHSWKNLGELVDIFLIG
                     DDEGIAQAAQQLQVKHFPHVCCNDRGTPLISSMLEIARQNSQSSYLCIINTDILVMPD
                     MLEGLRVVAERFMKFLLVGQRWDVAVTDPLPQDASLYPVVREKIHTVGRLHPPMGSDY
                     FLFPRNCFTKIPDFAIGRAGWDNWMIYKARLEGWKVVDGSRAVTIAHQDHDYSHFKDG
                     KPHYRQPETRINVQLAGGDQTVFTLHDAQYVIVGERVKRIPLTWKRFWREAEIFPITF
                     LKSQFLGKVSFYLFHPKKCYARLRTHWKRERQG"
     gene            complement(4776..6671)
                     /locus_tag="PLL88_03915"
     CDS             complement(4776..6671)
                     /locus_tag="PLL88_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ00740.1"
                     /translation="MSSRKIAPFFIVSCLCIATTLNACSPTQPAQSPSDEAPVEKSAA
                     AETRKITICLGYQPASLYIYKAATQVEWDVLQAIYDGPFDTGQGETVPVILQTVPTVQ
                     NGGVQIGQVSMQAGERIIDATGKPSTLAAGVTYLPSGCTTSDCAITWNGSDAVLVDQT
                     SMTFHFIDPLFWSDGTPLTAEDSVYSYQLATDPSTPNEKTISDQVDSYAAPDAHTIQV
                     QLIPGYIPANTEAYFFTPLPAHAWGQYTAAELLDSDLTNRTPLGWGAYSIQEWNEDSI
                     TLEKNPYYFRSGEGLPYFDELEFRFVSNLGDTNVASLEFDYEPYEILEYNWSPDGKTV
                     YSDQCDLIDATVDFSDQYDVFDYLTDYYITPAIQVHALPNSILDGLWLNPERPLVTPL
                     QPILSMCIDRTYVNSKTNYKLAFPTNSIYEQQPSSEEHGYDPHTASRLLDEMGWIDED
                     ENPATPRISRGNPLVADGIPLSMTMLVPYTPFYERESASVQASLQDCGIQLEVVFVNA
                     WDFYAEDSPLSTLDFDLIPVAQTISEGFPCSSLNEPWIGSILPAINEVQALSQLCSTI
                     PAAQTGNFADAAKEAVSNAFPFIPLFYRADVSITRSDMCGFIPTVGSSSDLWNLEEFN
                     YGVACAE"
     gene            6760..7704
                     /locus_tag="PLL88_03920"
     CDS             6760..7704
                     /locus_tag="PLL88_03920"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ00741.1"
                     /translation="MRVGQNPAKNIKNVVKPQRITIAVLNYIPNQQGYFADSFAVLQR
                     CLESLIAHTEQPYDLMVFDNGSCSKVKQYLLEMQAHGQIQYLVYSEKNVGKGGAWEFI
                     FNAAPGEIIAYSDNDAFFYPGWLKESLKILETYPRVGMITARPLRTIPELSTHTVRWA
                     QETKDVIVEEGDFISYEEYYEFSSTLGYSEEKLRKEYQSKKDTRLTYKDVQAFVGANH
                     FQFVGWKKTLQSIMPFDMQKPLGQVKQLDERLNEAGYLRLMITRPLIQNMSNKVSERL
                     NKSGKIHAKRTLRVKDIPLVKKTLLRIHDRIFTIYYGK"
     gene            7694..9169
                     /locus_tag="PLL88_03925"
     CDS             7694..9169
                     /locus_tag="PLL88_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00742.1"
                     /translation="MANENLNFNKKKKYTIFISADHGLAIVYFLQTQVVPALIYEDVE
                     VVVLTDDELVDKIQSRFGQPGLTVEGLRLKAVKDYLNEYKPSKQWWFNFFRRVGASKK
                     INTEALDSHIRQVEEEATGTRELLVGFIRIIVALLRNSRLARKWLVRNQMRLVPREKG
                     IYSDLFDKFDPDLVVASTPAWRIDRILVREAALRGIPTASVIVGWDNPSSYSLNGGKV
                     DWITCWSEIQKEELVQGSDWEPERVNIGGIPSYDGYFQHKWLMKKEDYFRDHNLDPRR
                     KLLGYACSFTTFSPNYQNIEAIVELMEADELVEPCQLLIRLHPNHFLDDPLFQAEKEH
                     IINLAKRSSHVHVVEPVPLGGDLGYYSGEDMPEKSSMMAYSDVFLTVYSTMVVEASIH
                     ETPIVSVCFDVPGGWGEKSRFYLPLSKIGNWPTHKRFIDSGAGRVAFNKQELKEHINT
                     YLRHPEIDLERQHTFIKNECTYINADAGRRTAGFLLSLIRQ"
     gene            9185..10171
                     /locus_tag="PLL88_03930"
     CDS             9185..10171
                     /locus_tag="PLL88_03930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOJ00743.1"
                     /translation="MPRIGMNPSRGQSTDYKPARVSLVMLTYIPNDIGYFEKRFEVMR
                     ASLESYIQNTGLPHDLIVFDNNSDEKVVDYLKEMRDKGKIDYLILSGRNIGKMGALQI
                     AFRAAPGEIIAYSDDDVFFLPGWLERHLEVLETYPKVGMVTGLYIRLHMKEGIGSVLK
                     FAEEEGVETRRGDLISKDLQFHYIDNTGRTWEQYQKETEGLEDLALTYKGVATLASAG
                     HYQFVARKEIILQALPGVWQNQLMGKMRELDLQVDLMGYLRLCTHPATIRLLGNVINP
                     EMAELINQYGISVAPSEIESQPVTLKTRFMRLPIVKRAAYFLYNRLFKIINA"
     gene            10216..11226
                     /locus_tag="PLL88_03935"
     CDS             10216..11226
                     /locus_tag="PLL88_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ00744.1"
                     /translation="MKILIAGLGSIGRRHLRNLVRLGEQDILLFRTHHATLADEELAG
                     YPVVTDLEKALGQKPDAAVISNPTALHLSVALPCAQAGCALFLEKPLSVDCEGLDELQ
                     DVLRKKGNPALVGFQFRFHPAFCQIAQWIKDGLVGKIYSAEVYWGEYLPDWHPWEDYR
                     VSYSARKELGGGVLLTLCHPIDYIRWLLGEVKSVSGFVSQVSDLELEVEDVADIRMRH
                     VSGAYSHIHLDYFTRPKRHTLQIIGSHASISWDETSNEIRLYQPNEKGWSTIRPPADF
                     ERNDLFLTEMRHFLNVIKQREKPACSIADGIRVQQIIMAAYQSAEQQQLEVALPEIQG
                     IK"
     gene            11223..12038
                     /locus_tag="PLL88_03940"
     CDS             11223..12038
                     /locus_tag="PLL88_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ00745.1"
                     /translation="MNRNKIEQVFNLREKVVLVTGAAGLIGSRFCQVLAQAGARLVAA
                     DIQVQKVGALVQDLQRSGLKAQSFPTDITSQESVRALLAQIKEQFGQLDVLVHCAAMD
                     PKFDKVNKQKQTVGFEEYPLELWQQAMHVNLTGTFLCVQEAARLMVQQGWGNMVLVSS
                     IYGMVAPDQRIYAKGFFKPPYYSVSKAGILGLTRYVASYYAGKNIRVNALSPGGIYHD
                     HDELFVREYSNRTMLNRMADVSELEGALLFLASDASSYMSGANLVVDGGWTAW"
     gene            12061..13290
                     /locus_tag="PLL88_03945"
     CDS             12061..13290
                     /locus_tag="PLL88_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HOJ00746.1"
                     /translation="MEKKKPEVLAIIPARGNSKSIPRKNIREFAGYPLIAYSIAAAMQ
                     ARSVTRVIVSTDDVEIANIANQFGAEIPFMRPDEIAQDQTADLPVFEHALGYLHEHEN
                     YQPDVVIQLRPTSPVRPTDCVDSAVSILLAHPDADCVRGVVIAGQNPYKMWHIQPNGV
                     LEPILTVEGVPEAYNAPRQELPLVYWQTGHIDAIRPATILQKRSMTGSVIYPLIIDPS
                     FTVDIDTMIDWRNGEQLIMEGGLDMVFPARPRREFPQKVSLILMDFDGVMTDDRVWVD
                     ENGREMVAAHRGDGMGLAMVKQKLGIDVIVISKEKNPVVARRCEKLHLHYHQGVNQKA
                     EVIDTILADRNLERDEVVYVGNDINDLDCFEKVGFAVAPADAHEQVKRKVDLILHKPG
                     GMGAVREFCDMLLARFS"
     gene            13398..14459
                     /locus_tag="PLL88_03950"
     CDS             13398..14459
                     /locus_tag="PLL88_03950"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOJ00747.1"
                     /translation="MIDIHFYQKVYDLKYWVMKGEKFEREYLLQKLEEFRSPQPVLYN
                     IETTNQCNMRCEMCPRTTIMTRPIETMESELFKRVIDQQRPWTETEWNGWETFVTENY
                     RIKPDDMSENHFFLYVIPKVIVLHGYGDPLLDRQIPQYVKWMTEKGIPSYFSCNPANI
                     NMNRTIETFENGLDYIKYSVESVDDTRHKQIRGYLSNFSESYANILELLNIKAQKNLS
                     TTIVITMLNLNKPNQEEEFERLKEKFKGLDVYIYLKSQDQQWYEDNKAGTNSIHWIEF
                     CQFPWSSMTIKSNGEACECVEDFNNEIILGDAHTQSLYDIWNGEKYKKFRMNHFDLTP
                     GIKCTEQCDMNLIGSFLVS"
     gene            14482..15354
                     /locus_tag="PLL88_03955"
     CDS             14482..15354
                     /locus_tag="PLL88_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HOJ00748.1"
                     /translation="MNRELKIGNKLVGDGHPTYVVAEIGINHNGDLQIAKDLILAAKD
                     AGVDAVKFQKRTPEICVPKEQQNQMRETPWGYITYLEYRYKVEFGEEEYRIIDELCKE
                     IGIDWFVSVWDTASVDFMEKFSPVCYKLPSASLTDDELLLKVRHTSRPLILSTGMSTS
                     EQIDHAVKVVGLDNLLITHSTSAYPCNPSELNLKMVETLRNKFPCPIGYSGHEVGLVT
                     SVVAVAMGACLVERHFTLDRAMWGNDQAASVEPVGFRKLVRYIRVTEQAMGDGIKKVY
                     DSELSSLKKLRRVQ"
     gene            15460..17013
                     /locus_tag="PLL88_03960"
     CDS             15460..17013
                     /locus_tag="PLL88_03960"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00749.1"
                     /translation="MNQTRISRISRQIPDNDPEHPAQPPLILFNASTRLEGISLNAGF
                     SLITGWALRMAGYPVIHFVCARGMSRCVLGTDRTDPIAAPPCSACIRTSKAVYRNCSV
                     RAFEFEMDKTLKDKLAGLSLGQLMKVKLDGMPVGSLILPSMRWILRKHHLEDDEDTRQ
                     LARHYILSAENIMREFNLLLDQAQPQAVVVFNGMFYPEAVARFLAQKRGIPVYTHEVG
                     MLPLSAFFTSGEATAYPIEIPDSFRLSRKQDGQLDAYLTQRTEGKFTTAGIEFWPEMR
                     ALDAAFAREMQKFKTVVSIFTNVIFDTSQTHANVVFPHMFAWLEVVLKSIRAHPEVLF
                     IIRAHPDELRAGKESAETVADWIRDNHVLDLPNVRFINARDYISSYDLINASKFIMVY
                     NSTIGLEASILGKPVLCAGKARYTQVPTVFFPKTKDAFHAMLEDFLTNDPIHVPEEFQ
                     SNARKVLYSQLFMASLPFETFLKEEKHWRGYVGVRNFKIDALKAENSPTIRTILHGIQ
                     KKEQFLRKP"
     gene            17010..17990
                     /locus_tag="PLL88_03965"
     CDS             17010..17990
                     /locus_tag="PLL88_03965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOJ00750.1"
                     /translation="MNPLCSIVIRAFNEEKHIGKLLQGISSQSVESVQVILVDSGSTD
                     HTVAIAESFGAKIVHIQPGQFTFGRSLNLGIAQAKADFVVLASAHVYPVYPDWLEKML
                     EPFRDDKVALVYGKQSGCATSKFSELQIFRCWYPDTSNFDQSTPLCNNANAAIRKSLW
                     EKHPYDEELTGLEDLAWATWAKEQGHKIAYSAEAEIVHVHQETWKGIYNRYRREGMAF
                     KRIHPQATFTFMEFIRVLCQNIWNDWREARMQRRLRKEFCRIVQFRFCQFWGTYQGYR
                     QSGPLTWQLKRSFYYPRIEPEVDNRNGRQGVQPISYGKDTTPEKSKESER"
     gene            17987..18673
                     /locus_tag="PLL88_03970"
     CDS             17987..18673
                     /locus_tag="PLL88_03970"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HOJ00751.1"
                     /translation="MKFEKIVALVPMRHHSQRVPQKNFRDLAGKPLYMHILTSLTKCK
                     LINQIVVDTDSPTIKEGIAENYPQITVLERPEHLRADTVPMNEILFYDTEQIAADLYL
                     QTHSTNPLLLTETITKAIQDFSDAYPAFDSLFSVTRWQTRLWDGLARPINHNPAILLQ
                     TQDLPPVYEENSCMYLFTREGLLRKRTRIGDRPYMFEVSALEAQDIDEEQDFLIADLL
                     MKYRLRNELL"
     gene            18701..19681
                     /locus_tag="PLL88_03975"
     CDS             18701..19681
                     /locus_tag="PLL88_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HOJ00752.1"
                     /translation="MPTVLLSAPYMLPSVERFKPVFNHYGIELVIPEVRERMEEEQIL
                     EYAGQFDGTICGDDRYSEKVIQKCLPRLKVISKWGTGIDAINKPYAESMGVMVGNTPN
                     AFTLPVSDSVMAYLLAFARKQPWMDRAMKDGIWEKIPGRSLSECVLGVIGVGNIGKAV
                     IRRAKAFGMRILGNDIQPIAPDFILENHIEMLLLDELLTQADFISLNCDLNPTSFHII
                     NAHSLEFVKPEAVLINTARGPLVDEPALIAALQGERLGGAALDVFEIEPLPSDSPLRK
                     MENVMLAAHNSNSSPSAWERVHWNTIRNLLIGLHIEHADLEDLQHKYALQ"
     gene            19694..20452
                     /locus_tag="PLL88_03980"
     CDS             19694..20452
                     /locus_tag="PLL88_03980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ00753.1"
                     /translation="MAKEKVVLITGAAGGIGSATVMKFARSGWDVIGVDRREFAGRFP
                     SDGLFILADISKPKDISLIYEKTSDFSAHLNAVVNNAAIQVSKPILDTTIDEWDLVIA
                     SNLRSAFVGAKFAHPLLVKAGGGAIVNVSSVHAVATSVNVAAYAASKGGLLALTRAMA
                     IEFAKDGIRVNAVLPGAVDTPMLRSGLDRGQHGGSNLDERLENLARKTVNGRVGKPEE
                     IANTIFFLATEESAFMTGQALVVDGGATARLSTE"
     gene            20456..21265
                     /locus_tag="PLL88_03985"
     CDS             20456..21265
                     /locus_tag="PLL88_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HOJ00754.1"
                     /translation="MREEIKKRVPEGLLNIGRATRDWIDRFPEDVSACTHPWRLDSQR
                     KLRKYHNRYQGKRCFVIGNGPSLKNTDLTALSNEYTFGMNRIYLMFPELGFPTSFLVC
                     VNDLVIEQTVEDMQKLAVPRFFSWRSRAWLRPADDLHFLHTTYTGPRFAKNAAGRLWE
                     GATVTYVCLQLAYYFGFTEVILIGVDHNFVTQGKPNTTVVSQGDDPNHFHPAYFGKGF
                     RWQLPDLETSELAYRMAKQAYEADGRRIVDATVGGKLTVFPKVQYSDLFKG"
     gene            21269..22222
                     /locus_tag="PLL88_03990"
     CDS             21269..22222
                     /locus_tag="PLL88_03990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ00755.1"
                     /translation="MRLLVSIVTPSYNQGAFLEATITSVLDQDYEALEYILVDGKSTD
                     GSVDIIKKYSDRLAWWVSEKDQGQADAINKGLSRVRGEIIGWLNSDDVYLPGALQTVA
                     TFFQDHPDVGLVFGDLLAVDENGEQINILRYADYTLQDLMAFRIIGQPAVFFRKEVLK
                     EAGFLDLSYHYLLDHQLWIRMASFTNIRHIPQVLAGARFHQGAKNVAAAEQFGEEAYR
                     IVNWMRSYEKTSSILPGIEKKVLAGACLIDGRYLLDGGKPRQAFKVYARSLFLHPATG
                     FREFHRMLYALAATLGLVRVQSVVERRRKLNTIKARFHRKQ"
     gene            22245..23726
                     /locus_tag="PLL88_03995"
     CDS             22245..23726
                     /locus_tag="PLL88_03995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ00756.1"
                     /translation="MKNKSFFVLAFLLIILGLAIRLFDLDDAPLDFHPTRQLHSALMA
                     RGMYYQLTGDASEKAAVAIRQWKLEGLIEPPIMEWLAAFGYKLAGGVDLRIPRLIAIL
                     FWLLAVIPLLVVAKRGAGWKAALVGAGFFLFIPYGIIASRSFQPEALLVLLLALFFMA
                     LSLWDNDRSWKWAVWAGVLGGLSVLIKTVAAFFVAGMWLGWLLSDENWKTAIKEKKMW
                     VAFLLTVLPYAAYLAYGLWIDKSYAGQFSLRFFPSMWTELSFYLRWLSNLRRVVDVGW
                     LVLGIIGTLLLEKKSLRNILVGAWMGYILLGFVLPYHISTHDYYHLPLLFLVALGLCG
                     LFAGIFSLLEKRSAVLKGMGGVCLLLLFGVYAMDARSELKKVNYSAEVSFWERVATLF
                     SPADRVIALSQDYGNRLAYWGWLTPRNWPSLDDIELRRETGQDLTLEDYFADYTDGYD
                     YFLITDFDEFARQPQLEEWLHDKYPLLFTDEGVLLFDLTPGSP"
     gene            23741..24490
                     /locus_tag="PLL88_04000"
     CDS             23741..24490
                     /locus_tag="PLL88_04000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ00757.1"
                     /translation="MQSDKNTNPQISVVIPAYNEQNWIELTLQSLVSFLGKEFNGWEI
                     IVVDDGSEDDTQKICERFSQEIAQVVYLRNAKNSGKGFSVRRGMQAARGEKRFFMDAD
                     MPFELDVLLTMYNALATECDISIGARDLPGSTLIGVPFTRFLAGQIFSLLVRLFAVKG
                     ISDTQCGLKGFTAQAAEAIFSRTTINDFGMDAEVLFIAQHLDYRIQRVPVRMTGFRGN
                     SRVHLLKDSVNMFFELLLIRWKALRGVYRLR"
     gene            24559..26013
                     /locus_tag="PLL88_04005"
     CDS             24559..26013
                     /locus_tag="PLL88_04005"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HOJ00758.1"
                     /translation="MMRRFQCPLDKQKTERLFRSVLVVANLLLFIALAVYAWRGFYSR
                     YWADDYCFGSISHAHGLLSGTRYFYQNVSNRFAAYFLVGLNELFGESAIRFLPAFMIM
                     VTVVIYTKVFQLACQIVRFPQPWYVSLFLSQTLTYFMVLMAPNLFQSLFWRSGMVSYF
                     APIPFFGLSLILLVSGLMKGFPWYRLDGLWLIAFFSAGLSETFAAMETGVWVILTLGG
                     FLFLSRKERQPLMKGALPALIGSLAAMGMMILAPGNSMRLATLEQASNPWQVIVLSLR
                     FAASFSYHTLVGLPIPLLVLFAVVLIVAYHLSGHTQPALQDAQWMKLFWGSLVAGFIL
                     LVCVAAPTAYGMLAYPEERAWMLGRFVTVLVLIVAAFSAGVLSHRWMDRIVDTCLMSI
                     IVLFLLSLYPLKGAWSELQQLPQWETVAHAWDVRHAQIKEKVVAGETIFTVQAFDSIG
                     TVAELTANPDYWVNVCAADYYGVEEIAAVEGFYE"
     gene            26006..26779
                     /locus_tag="PLL88_04010"
     CDS             26006..26779
                     /locus_tag="PLL88_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ00759.1"
                     /translation="MSDLPLVSIVTPSYNQAPYLEKTIISVLEQDYPNLEYSIVDGGS
                     TDGSLDLIKKYASRLGWWISEPDQGQTAAINKGFSHATGKIYAWLNSDDTYQPGAVRE
                     AVDFLLRNPQAVMVYGDAHYIDERDRVIGNFPAAQTDLKRLRRGYVHIPQQASFFTAE
                     GWKAVAPLDESFYFAMDYDLWVRLARLGPIVYHPRVWANFRLHQDAKTIAADEHCWPE
                     MLRVHYRDGGSHFAPIVWKSWLRKLAAPYIRWKRKKMFI"
     gene            26794..27084
                     /locus_tag="PLL88_04015"
     CDS             26794..27084
                     /locus_tag="PLL88_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00760.1"
                     /translation="MRDINFFKDLVFKFRLSVLLMLDKRVAVYLKLIPIAALIYLVVP
                     FDLVIGPIDDAALVLGAMQLFISLCPKELVEQHSQGLEKRKEAAKTSSTKFS"
     gene            complement(27516..28037)
                     /locus_tag="PLL88_04020"
     CDS             complement(27516..28037)
                     /locus_tag="PLL88_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ00761.1"
                     /translation="MPHYHRYYIPNSYIFITVVTKNRFPYFNNLKNIQLYFTTLEKVK
                     IIHKFDLYAYVLMPDHFHWILKMENENGNFSQVIHCFKRNYTLNYKSAFSITNPTSLW
                     QKRFWDHIIRDETDMKRHLDYIHWNPVKHGASNDPNGYKLSSFHDFVSQDLYPENWGR
                     TGIPISIENIDFE"
     gene            complement(28269..>28591)
                     /locus_tag="PLL88_04025"
     CDS             complement(28269..>28591)
                     /locus_tag="PLL88_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ00762.1"
                     /translation="LEESTELVDTLWANGAYFKREMSGMGFDIGNSQTPITPVMLGEA
                     QLAQDFSKRLFERGIFATSIGYPTVPQGKARIRVMISAAHSKDDLDRGLEEFKTVGKE
                     LGVI"
BASE COUNT         7124 a         6361 c         7562 g         7544 t
ORIGIN      
        1 tctatgcagt tgacctgttt gaggaaaaga atgcgcgctc gctcattgat gagcataagc
       61 cggatgtcct tgtcaattgt gccgcactgg cggatgtaga tgcgtgtgaa aacgatcatg
      121 tgcgcgctcg tcggttgaac gccgaattgc ccggttggct ggctgaggag tgtgaacgtc
      181 atgcggtaaa actcctgcat atttctaccg atgcggtttt tgatggtcga aaggaaggaa
      241 aatatcgaga aggggatgag gaacatcctc ttggaatata cgcgcaaacg aaactggcgg
      301 gagaaaacaa tgtcttgcag gtgaattccg atgcgctggt tgcaagggtc aatttctacg
      361 gcttcagtat ttctaagaag cgcagtctgg cggaattttt tctccatagt ctgcaggaag
      421 gaagagccgt ccacggattc accgatgtgc tctttagccc gttatatgtg gttgatctgg
      481 cacaaatatt gttggaaatg attgaaaagc aacttggcgg tctgtaccat gtcgtcagcc
      541 ctgaatcgca aagcaaatat gcatttgggg taagcattgc gcatcgtttc ggatttgaag
      601 aaaatttgat ccagcccatt actgtagctg aagcgacctt tctttctgcg cctcgttcta
      661 agaatctgat gctggaccct acaaaaacgc aaaatgcgtt gaacagaaca ttacctggac
      721 agttggaagg gctcgaacat ttctatgggg attatacagg cggattggcg gagcgcattt
      781 atgctttaca gtgagatttg aaagggtgaa aggagagtac gatgattttg atgataggga
      841 atcataaaat tggaatagag caccccacat atttcatcgc agatattgcc gccaaccatg
      901 atggcgacct ggagcgtgcc aaatcactga tcagactggc aaaagaagcc ggggcggatg
      961 cagcgaaatt ccaaaacttt caagcccaca agatcgtctc tgattatgga ttcaaaacca
     1021 tgggcgggca gcaatcgcac caggcgaaat ggaagaaatc ggtttttgaa gtgtatcgag
     1081 atgcatccgt gcctttcgat tggacaccca ttttgaaaga ggaatgcgat aaacaaggga
     1141 ttgactattt ttcttcaccc tacgattttg aagcggtgga tatgttggaa aattatgtcc
     1201 cggcatacaa agtgggttca ggagagatag attggattga agctttggaa tatatggcat
     1261 ccaaagggaa accaatggtc attgccaccg gggctgcgac cattggggaa gtgcaaaagg
     1321 ctgtgcatgc catcctgcat attaacccaa atctggtctt aatgcagtgc aacaccaatt
     1381 acactgccag ccttgagaat ttcaagtata tcaatttgaa cgtgttgaag acatatgcgg
     1441 tgatgttccc tcaagttgtg ctggggcttt ccgaccacac accagggcat gcgaccgtgc
     1501 tgggggcagt aacgttaggc gcgcgagtga tcgaaaagca tttcacggat gataatgatc
     1561 gtgaaggtcc tgatcacctt tttgccatga accctgaaac ctgggcggaa atggtggaga
     1621 acacgcgacg cctggagcag gcgctgggtt cggcagataa gtttgtcact gagaatgaaa
     1681 cagaaacagt cgttatccaa cggcgttgtt tgcgcgctgc acgcgatatt tccaaaggag
     1741 aggtcctgac acgctcgatg atcgatgtgt tgcgtccggc aacgcctgga gcgatcctgc
     1801 cggatcaact gccaaaagtg cttggtttgc gcgccgttga gaacatccca gccgggcggg
     1861 aaatttgttg gatcgacctg ggtaaatagg tagggtgtgc tggtgcgcgt tctatactgt
     1921 tctcaggatt attctccgca cgaccaccgc tttcttagtg cgctttccaa tacggaacac
     1981 cagatttact ggctgcgcct ggagaaccgc cggcgaaatt tggaggtgcg acccttaccc
     2041 gaaagtgtcc atcaggtaac ctggcgaact ggcgatgaaa aggtgcattg gtggcagtat
     2101 ttaggcttgg caggaagatt caggaaagtt ctgaacgcca tccagccaga cttggtgcac
     2161 gccggaccga tccagccggt gagtgtcctc cccgccatgg ttggatttca cccgctggtg
     2221 agcatgtctt ggggttttga catcctgcaa gatgccactc gaaattcctt ttggaaatgg
     2281 gtgaccggat ttgtcttgca tagaacagac cgcctgtttt gtgattgcca ggccgtgaag
     2341 aaaaaagtgg tcggctgggg gtttccggaa agaaaaataa cggttttccc ctggggagtc
     2401 gacctgacgg tgttcaaacc tgacgtatcg aaaaagaaga tcggtgaagc gatcatcttt
     2461 ttaagcaccc gttcttggga accgcgttat ggcttggatg tcgcactcaa agggtttgcc
     2521 agcgctgtgc gagagaaacc aaatttgcgt ctcgttatgc tcagcgacgg gtcgcaagcg
     2581 ggttggatac acaatttcat cagagaaaac cagcttgaac aacatgtaga gctgcgcggg
     2641 cagatcggga atgattcact gtctgatatt tatcatcagg cgcatattta tctcagcgca
     2701 tcgcatgtgg atggttcctc ggttgcgctg atggaagcgc tggcatgcgg aacccctgct
     2761 ctggtttcag atatcccatc caatctggaa tgggtcaaag atggggcaga aggctgggtt
     2821 ttcaaagatg gtgatacggc tgacctggcg cgagtgatgg tgcacattgc ctcgcaccca
     2881 gaattgattc acgaagcatc gctgatggcg aggaaaaagg cgcagcaaaa tgcggattgg
     2941 aacaagaacg ttgctttgct tctcgatggg tataagcgcg ccatgaagga ggttgtggaa
     3001 tgaaggaacc gagagttgtt gccatcatcc aggcacgcat gggttctaat cgcctgccgg
     3061 ggaaagtgtt gcgtgatatt catggaaaac cgatgctagc ctgggtggtg gaacgcgccc
     3121 gtctggctgc tacgcttgct gaagtagtag ttgctacgac tacagacgcc ggtgatgaca
     3181 tattggcaca ggtgtgtaaa gaaatgggag tgacttgttt tcgcgggagc acctttgatg
     3241 tgctcgatcg ttattatcag gcagccagag aaagtgaggc agatgtgatc gtgcgattga
     3301 cggctgattg cccgttgatc gatccaactt tgattgatct ggtagtgacc cgcttttttg
     3361 aggagaaggt agattttgca acaaaccgcc tgccgccgcc ctatcaacgc acttacccga
     3421 ttgggttgga tgttgaggtg gtttcgtttt ctgctctgga gaaagcttgg aaggaagcct
     3481 tggaggtgca tgagcgagag catgtgatgc cgtatttcta tgacgccccc ggtcgtttta
     3541 aaatcctcat tgtggatcac gagacgaatc tcgggaaata tcgctggacc gtggataccc
     3601 agcccgattt gcgatttgtt caggaggtca ttgctcgttt gccagataag gagcacttct
     3661 cctggttgga tgttttgaag atcgtcgaag agcaccccca actatcagag atcaatgccg
     3721 gaatcgctca taagacttat ctggatgtgg acggacatgc gacgaacaca ggagagaacc
     3781 atgcctgatc cgcttgtgac cttctttact gcacccaaac cgttcaccaa tccccatatc
     3841 aatatcatcc aacgcaatgc tatccattcc tggaaaaacc ttggagaact ggtggatatc
     3901 ttcttgatcg gcgatgatga agggatcgca caggctgccc aacaactcca ggtaaagcat
     3961 tttccacacg tgtgctgcaa tgatcgggga acacccctga tcagttccat gttggagatc
     4021 gcccgccaga atagccagag ttcttacctg tgcatcatca acacggacat tcttgtgatg
     4081 ccggatatgc tagaagggtt gcgggtggta gctgaacggt ttatgaaatt tttgctggtt
     4141 ggacaacgct gggatgtcgc cgtcacagat ccgctaccgc aagatgcttc gctgtatcct
     4201 gtagtgcggg aaaagattca taccgttggc aggttgcatc cacccatggg aagtgattat
     4261 tttcttttcc cacgcaattg cttcacgaaa attcctgatt tcgccatagg gagagccggc
     4321 tgggacaact ggatgatcta caaagcgcgc ctggaggggt ggaaggtggt agatggcagc
     4381 cgtgccgtta ccatcgccca tcaagatcat gattacagcc attttaaaga tggcaaaccc
     4441 cattatcgcc agcccgaaac cagaatcaat gtgcaattgg caggggggga ccagaccgtt
     4501 tttacattac atgatgcaca atatgtcatc gtaggtgagc gggtgaaacg tattccgtta
     4561 acctggaaac ggttctggcg tgaagcagag atatttccca tcaccttctt gaaaagtcag
     4621 tttctcggaa aagtctcgtt ttatctgttc catccgaaaa agtgctatgc ccgcttacga
     4681 actcattgga aaagagagcg ccagggatag ctttatgggg gattgctcca attttttata
     4741 acggttctgt tgtagacact cagaacccgc ttcttttact ctgcgcaggc aactccgtaa
     4801 ttgaattctt ccagattcca tagatcgctg gaggaaccaa cagtggggat aaatccgcac
     4861 atgtccgaac gggtgatgct gacgtctgcg cggtagaaca atggaatgaa ggggaaagca
     4921 ttcgataccg cttcctttgc agcatcagca aagttaccgg tttgggcagc ggggatcgtg
     4981 gaacaaagct gtgagagtgc ctgtacttcg ttgatggcgg gcaggatgct ccctatccat
     5041 ggttcgttca aactgctgca gggaaaaccc tcactgatgg tttgagcgac cgggatcaaa
     5101 tcaaagtcaa gtgtagataa agggctatcc tctgcgtaaa agtcccaggc gttcacaaaa
     5161 actacctcca gttggatgcc acaatcttgc aatgatgcct gaactgaagc cgattcacgc
     5221 tcatagaaag gggtataggg aaccagcatg gtcatgctca aggggatgcc gtctgcaacc
     5281 agcggatttc ccctggaaat gcgcggcgtt gcggggttct catcctcgtc gatccaaccc
     5341 atttcatcta gcaacctgga ggctgtatgt gggtcataac catgctcttc tgatgatggc
     5401 tgctgttcat aaatagagtt tgtcgggaat gctaatttgt agttggtttt actgttcaca
     5461 tatgtgcgat caatgcacat ggaaaggatc ggttggagtg gggtaacgag aggtcgctca
     5521 gggttcagcc acaatccgtc gagaatggaa ttcggaaggg cgtgtacctg aatggcgggg
     5581 gtgatgtagt aatcggtaag gtaatcaaat acgtcatatt gatcggagaa atcgacggtt
     5641 gcatcaatca gatcgcattg atcggagtag accgtttttc cgtccgggct ccagttgtat
     5701 tccaggattt cataaggttc ataatcgaat tccagcgatg ccacattggt atctccaagg
     5761 ttggagacga agcgaaattc cagttcatca aaatatggca atccttcgcc agatcggaaa
     5821 taatagggat tcttttcaag ggtgatggag tcctcattcc actcttggat ggaatatgct
     5881 ccccaaccta gtggggtgcg gttggtgagg tctgaatcga gcagttcagc ggcagtatat
     5941 tgtccccagg catgtgcggg caagggggta aagaagtatg cttctgtatt cgcggggata
     6001 tatcccggga tcaattgcac ctggatggtg tgcgcatccg gtgcggcata gctgtcgacc
     6061 tgatcgctga tggttttttc gttgggcgtg gagggatcgg ttgccagttg ataagaatag
     6121 acggagtctt ccgcagtcag cggggtgcca tctgaccaga acaaggggtc aatgaaatga
     6181 aaagtcatgc tggtttgatc aaccagaacc gcatcagacc cgttccaggt gattgcacaa
     6241 tcagaggtgg tgcagccact gggcaaatat gtgacgccgg cggctaacgt gcttggtttt
     6301 cccgttgcgt ctattattcg ttcccctgct tgcatgctga cttgcccgat ttggaccccc
     6361 ccgttttgta cggttggcac tgtttgcaga atcaccggaa ccgtctctcc ttgacccgta
     6421 tcgaacggac catcatagat ggcttgcaga acatcccatt caacctgggt tgctgctttg
     6481 taaatgtaca atgaagcagg ttgataaccc agacagatgg tgatttttcg cgtttcagcg
     6541 gctgcgcttt tttctacagg tgcctcatcg gaaggactct gtgcaggttg agttgggcta
     6601 caggcgttca gggtggtggc aatgcataaa caactgacga tgaaaaacgg ggcgattttt
     6661 ctactgctca ttttgtgatt atacgataat ttctcttttt gccgttggat tggtacaatc
     6721 attctatggt agcattaagc ccgcttttgg agatttcgca tgcgcgttgg tcagaatcct
     6781 gctaagaata ttaaaaatgt tgtaaagcca caacggatca cgatcgcagt tctgaattac
     6841 attcccaatc agcagggtta tttcgctgat tctttcgctg tcttacaacg ttgtctagaa
     6901 agcctcatcg cgcacactga gcaaccatat gatctcatgg tttttgataa tggcagttgt
     6961 tctaaagtaa aacaatattt gctcgaaatg caggctcacg gtcaaatcca atatcttgtt
     7021 tattcagaga aaaatgttgg caaaggcggc gcgtgggaat tcattttcaa cgctgcaccg
     7081 ggcgaaatta ttgcgtatag tgataatgac gcctttttct atccgggctg gttgaaagaa
     7141 agcttaaaaa tactggaaac gtatccgcgt gtgggtatga tcaccgcacg accactgcga
     7201 actattccag aactctctac ccatactgtt cgctgggcac aagaaaccaa agacgtcatc
     7261 gtggaggagg gggattttat cagctatgag gaatattatg aattttcttc aacgttgggt
     7321 tattcagagg aaaaattaag gaaagaatac caaagcaaga aagacactcg attgacctat
     7381 aaggatgtgc aggcttttgt gggggcaaac cattttcaat ttgttgggtg gaaaaaaaca
     7441 ctgcaatcca taatgccttt tgatatgcaa aaaccgcttg ggcaggttaa gcaattggat
     7501 gaacgcctga atgaggcggg atatctacga ttaatgatca ccagacctct cattcagaat
     7561 atgagcaata aggtctcgga gcgtttgaat aaaagcggaa aaatccatgc aaaaagaact
     7621 ttgcgggtga aggatatccc tctggtaaaa aaaacactct taagaattca cgatcggatt
     7681 tttaccattt actatggcaa atgaaaacct taattttaat aaaaagaaaa aatatacgat
     7741 ttttatcagc gcagatcatg gattggcgat tgtttatttt ttacaaacgc aagttgttcc
     7801 agctctgatc tatgaagacg tagaagttgt tgtgttgacc gatgacgaac tggttgataa
     7861 aatccagtct cgctttgggc aacctggttt gactgttgaa ggattgcgat taaaagccgt
     7921 aaaagactat ctcaatgagt ataagccgtc aaaacaatgg tggtttaatt tttttcgcag
     7981 agtaggggct tcgaaaaaga tcaataccga agcgcttgat tcccatattc gccaggttga
     8041 ggaagaagct acgggcacgc gcgaactttt ggttggtttt attcgcatta ttgttgccct
     8101 tctacgaaat tcccgcctgg cacgcaaatg gctggtgcgc aaccagatgc gcctggttcc
     8161 cagggagaaa ggaatctatt cagatctctt tgataaattc gaccctgacc tggtggtagc
     8221 cagcacgccc gcgtggcgta tcgatcgaat ccttgttcgc gaagcagcgt tgcgtggtat
     8281 tccaaccgct tctgtgatcg taggctggga taatccctca agctatagcc tcaatggcgg
     8341 aaaggtcgat tggattacct gctggtcaga aatacaaaaa gaggaattgg tgcagggttc
     8401 tgactgggaa ccggagcggg tgaacatcgg tggcattccc tcctatgatg ggtactttca
     8461 acataagtgg ttgatgaaga aggaagatta ttttcgtgat cacaacctgg atccgcgtag
     8521 aaaattgctg gggtatgcgt gcagtttcac cacattttcc cccaattatc aaaatattga
     8581 agccatcgtg gaattgatgg aggcggatga gctggtggaa ccttgccagt tgttgatccg
     8641 gctgcatccc aatcatttcc tggatgatcc tttgttccag gcggaaaagg agcatatcat
     8701 caacttagcc aaacgcagca gtcatgtcca tgttgtggaa cctgttccgc tgggcggcga
     8761 tcttggctat tattcgggag aagatatgcc tgagaaatca tccatgatgg cttattccga
     8821 tgtgtttctc acggtttatt ccaccatggt ggttgaagct tcgatccatg agacgccgat
     8881 tgtgagcgtt tgttttgacg ttccgggtgg atggggcgaa aaaagcaggt tctatctgcc
     8941 actttcgaaa attggaaact ggccaaccca taaacgcttc attgattcgg gtgctgggcg
     9001 tgtggctttc aacaagcaag aattgaagga acatattaat acatacctgc gccatccaga
     9061 gattgacctg gaacgacaac atacctttat taaaaatgag tgcacgtata tcaatgctga
     9121 tgcagggcgc agaactgcag gcttcttgtt atctttgatt cgacagtaag ctaggagaga
     9181 tattatgcca agaataggaa tgaatccctc tcgaggacag agcaccgatt ataaacccgc
     9241 cagggtttca ttagttatgt tgacctatat ccccaacgac atcgggtact ttgaaaaacg
     9301 cttcgaggtc atgcgcgcca gcctggaaag ctatatccaa aataccggtt tgccgcatga
     9361 tctgatcgtt tttgataata acagcgatga aaaagtggtc gactacttga aagaaatgcg
     9421 cgacaaaggc aaaatcgatt atctgatcct ttcaggcaga aatatcggca aaatgggcgc
     9481 gctgcagatc gcttttcgag cagcgccggg cgagatcatc gcctatagcg acgacgatgt
     9541 tttcttcttg ccgggttggt tggagcgcca cttggaagtg ctcgagacct atccgaaggt
     9601 cggcatggtt accggacttt atattcgctt acacatgaag gaagggatcg gttcagtgtt
     9661 gaagtttgcc gaggaggagg gcgtcgaaac gcgtagagga gacctgatct cgaaagattt
     9721 gcaattccat tatatcgata acaccggacg aacctgggag cagtatcaaa aagagaccga
     9781 gggcttggaa gacctggcat tgacctataa gggcgttgct acgttagcct cagccgggca
     9841 ttaccagttt gttgcgcgca aggagatcat ccttcaagcg ctcccgggcg tttggcagaa
     9901 ccagcttatg ggaaaaatga gagagctcga cctgcaggtt gacctgatgg gatatttacg
     9961 attgtgcact caccccgcca ccatccgctt gttgggcaat gtcatcaacc cggaaatggc
    10021 agaattgatc aatcagtacg gtatttcggt ggctcccagc gagattgaaa gtcaaccggt
    10081 gactcttaag accagattta tgcggctgcc gatagtgaaa cgtgcggctt acttcttgta
    10141 caacagattg ttcaaaatca tcaatgcgta gagaaacctg atgcaaggct tgattacggt
    10201 atactggaat tgaatatgaa aattctcatt gcagggctgg gttccatagg gcgcaggcac
    10261 cttcgaaatc tggtgcggtt gggagaacag gatattctgc tctttcgcac ccatcatgct
    10321 acccttgccg atgaagagct ggcagggtat ccggtggtga ccgacctgga gaaggccctt
    10381 gggcagaaac cggatgccgc cgtcatttcc aaccccaccg ctttacacct gagcgttgct
    10441 cttccctgtg cgcaagctgg ttgcgcactg tttcttgaaa aacccctcag cgttgattgc
    10501 gaaggattgg atgaattgca ggatgttctg cggaagaagg gcaatcctgc attggtcggt
    10561 tttcagttcc gttttcatcc ggcgttttgc cagatcgcgc agtggattaa agatggattg
    10621 gtcggcaaga tctattccgc agaagtgtat tggggagaat acctgcccga ttggcacccg
    10681 tgggaagact accgggtttc atacagcgcg cgaaaagaat tggggggtgg ggtgctgttg
    10741 actttatgcc atcccattga ctatatccgc tggctcttgg gtgaggtgaa atctgtttct
    10801 gggtttgtca gccaggtcag cgatctggaa ttggaagtgg aggatgtggc tgatattcgt
    10861 atgcggcatg tgagcggtgc atattcccat atccacctgg attactttac ccgaccaaag
    10921 cgtcacacat tgcagatcat tggcagccac gcttcgatca gttgggatga aaccagcaat
    10981 gagatcaggc tctaccagcc aaatgagaaa ggatggtcga ccatcagacc ccctgccgat
    11041 tttgaacgga atgatctgtt tttgacagaa atgcgccatt ttttgaacgt gatcaaacag
    11101 cgcgaaaaac cagcttgttc tattgctgat ggaattcggg tgcagcagat tatcatggct
    11161 gcctaccaga gcgcggagca acaacaatta gaggttgcgc ttccggagat tcaagggatc
    11221 aaatgaatag gaataagatc gaacaggtgt ttaatcttcg cgaaaaggtg gttctggtga
    11281 ccggtgccgc aggattgatc ggctcaagat tctgccaggt cttggcgcaa gccggagcca
    11341 gactggtggc tgccgatatt caggtacaga aggttggtgc acttgtgcaa gacttgcaaa
    11401 gatcgggatt gaaagcgcag agttttccaa ccgacatcac ctcacaggaa tctgtaaggg
    11461 cgttgctcgc gcaaattaaa gaacaattcg gacagttgga tgtgttggtg cattgcgctg
    11521 ccatggatcc gaaattcgat aaggtgaaca aacaaaagca aaccgttgga tttgaagagt
    11581 atccgctgga actctggcag caagccatgc atgtcaatct gacgggaacg ttcctctgtg
    11641 tgcaggaagc cgccaggctg atggttcagc aagggtgggg caatatggtg ctggtttcgt
    11701 cgatctatgg catggtagcc cccgaccagc gtatttatgc aaaagggttt tttaagcccc
    11761 catattatag cgtttccaag gcaggtattt tgggcttaac ccgctatgtg gcgtcatact
    11821 atgccggaaa gaatatccgt gttaacgcgc tatctcccgg cgggatttat catgatcatg
    11881 atgagctgtt cgtccgtgag tactccaatc gtaccatgtt gaaccgtatg gctgatgtct
    11941 cagaattgga aggggcactg ctgttcctgg catcggatgc ttccagctat atgagcggag
    12001 cgaacctggt tgtggacggc ggctggacag catggtaagg ggagcgaagg ataataagga
    12061 atggaaaaaa agaaacctga agttttagca atcatccctg cgcggggtaa ttcgaagagc
    12121 attccgcgca agaacatcag agaatttgcc ggatatccat tgatcgccta tagcattgcc
    12181 gctgcaatgc aggctcgttc tgttacacgg gtcattgttt ctacggacga tgtggagatt
    12241 gccaatattg ccaatcagtt cggcgccgag atccctttta tgcgaccgga tgaaattgct
    12301 caagatcaga ctgctgatct gcctgtcttc gagcatgctt tagggtatct tcatgagcat
    12361 gaaaattacc agcccgatgt ggtcatccag cttcgcccaa catcccctgt gcgtcccaca
    12421 gactgtgttg atagcgcagt ctccatcctg ttggctcatc cagatgccga ttgtgtgcgt
    12481 ggggtggtga tagcggggca gaatccttat aaaatgtggc atatccagcc caatggagtt
    12541 ctggaaccga tcttaaccgt ggaaggcgtg cccgaagctt acaatgcgcc caggcaggag
    12601 ctccccctgg tttactggca aaccgggcat atcgacgcca tccgccctgc cacaattttg
    12661 cagaaacgat cgatgacggg aagcgtcatt taccctctca tcatcgaccc ttctttcact
    12721 gtggatatcg ataccatgat cgattggcgt aacggtgaac agcttattat ggaaggcggc
    12781 ttggatatgg tcttccctgc tcgaccccga agagagttcc cccaaaaagt ttccctcata
    12841 ttgatggatt ttgacggtgt catgaccgat gaccgggttt gggttgatga aaacgggcgt
    12901 gaaatggtag ccgctcatcg cggagatggc atgggtttgg caatggtgaa acaaaaactg
    12961 gggatcgacg tgattgtcat atccaaagag aaaaatcccg tggtcgcccg gcgctgcgaa
    13021 aaattacatc ttcattatca tcaaggagtt aatcaaaaag ctgaggtgat tgataccatc
    13081 ctggctgatc gtaacctgga gcgcgatgag gtggtttatg tgggaaatga tatcaacgat
    13141 ctggattgtt tcgagaaggt gggctttgcc gtggcgccgg cagatgctca tgaacaggtg
    13201 aagcgcaaag tcgacctgat tttgcataaa ccaggtggca tgggtgcggt gcgagagttt
    13261 tgcgacatgc tgctggcgag attttcataa cgatcactgt gcgaatcagc gtatgatttt
    13321 ctggctgaag aaaatcatac gacaggttat aatagctttt ttgtgagtca attgaataac
    13381 tttgtggaga ttaaagcatg atcgatatcc atttttatca aaaggtttat gatctgaaat
    13441 attgggtgat gaagggtgaa aagttcgaac gtgaatacct gctgcaaaaa ctggaagaat
    13501 tccgcagccc acaacctgtt ttgtacaaca tcgagactac caaccaatgc aacatgcgtt
    13561 gtgaaatgtg cccgcgcaca accatcatga cgcgccctat tgaaaccatg gagtcggaac
    13621 ttttcaagag ggtcatcgac cagcagcgac catggacaga aacggaatgg aatgggtggg
    13681 agacttttgt tactgaaaat taccgcatca aaccggatga tatgagcgag aaccacttct
    13741 ttctctatgt tatccccaaa gtgatcgtac tgcatgggta tggtgatcca ctgctggaca
    13801 ggcagattcc acagtacgtg aaatggatga cggaaaaggg aatccccagc tacttcagtt
    13861 gcaacccggc taacattaat atgaaccgta ccatcgaaac ctttgagaac gggcttgatt
    13921 acattaaata ttccgttgaa agcgtggatg atacaaggca taaacaaatt agagggtacc
    13981 tttcgaattt ctctgagagt tatgcgaaca ttctggaatt gttgaatatc aaagcgcaaa
    14041 agaacctctc tacaaccatt gtgatcacca tgctgaattt gaacaaaccc aatcaagaag
    14101 aggaatttga acgcctgaaa gagaagttta aggggctgga tgtttacatt tacctgaaaa
    14161 gccaggacca acagtggtac gaagataata aagccggcac caactccatc cattggatcg
    14221 aattttgtca gttcccctgg tcttccatga ccatcaaatc caatggcgaa gcctgcgaat
    14281 gtgttgagga tttcaacaat gagattatcc ttggtgatgc gcatactcag tcgctatacg
    14341 atatctggaa cggtgaaaag tataaaaaat tccgcatgaa tcactttgac ctgaccccag
    14401 ggattaagtg cacggaacag tgtgatatga atttgatcgg aagttttttg gtttcataag
    14461 aatggtgatg aggagacttg catgaatcga gagttgaaga tcgggaacaa actggtgggg
    14521 gatgggcacc ctacctatgt ggtggctgaa attggcatta atcataatgg cgatcttcag
    14581 atcgccaaag atctgatctt ggctgccaaa gatgctggcg tggatgcggt gaagtttcaa
    14641 aaacgaaccc cggaaatttg tgttcccaag gaacagcaga accaaatgcg tgagacaccc
    14701 tggggatata tcacctattt agagtaccgc tacaaagtcg agtttggtga agaagaatac
    14761 cggatcatcg atgaattgtg caaggagatt ggtattgact ggtttgtttc ggtttgggat
    14821 accgcgtctg tggatttcat ggaaaagttc tccccagtgt gttataagct gccctccgct
    14881 tctctcaccg atgatgagct gttgttgaaa gtgcgccaca ccagtcgacc gcttattctt
    14941 tcgaccggta tgtcaacttc tgagcagata gaccacgcgg taaaggtggt tggtcttgac
    15001 aacctgctca tcacgcattc aaccagcgcc tatccatgta atccttctga acttaacttg
    15061 aagatggtcg agactctccg aaataagttt ccctgcccga tcggttattc agggcatgaa
    15121 gtcggtttgg tgacctccgt tgttgcagtt gctatgggcg cctgcctggt ggaacgacat
    15181 ttcacgctcg atcgtgccat gtgggggaat gatcaggcgg cttcggttga accggtcggt
    15241 tttcgaaaac tggtgagata tattcgcgtg acggaacagg ctatgggaga tgggatcaag
    15301 aaggtatacg atagtgagct atcttcgttg aaaaagctgc gcagagttca gtaagacctg
    15361 aatctggtgg gcagcgttcc cgattcataa tacaatagtc ttgtggaaaa tccaattcga
    15421 cgagtttcca ggaaaattag gcgagaatat ctgcgtttga tgaatcagac gcggatttca
    15481 cgcatttccc ggcaaatccc cgataatgat ccggagcatc cggcacagcc gccactgatt
    15541 ctttttaacg cttccacccg cctggaaggg atcagtttga acgctggttt ctctttgatc
    15601 accggctggg cgttgcgcat ggcgggttat ccggtgatcc attttgtgtg cgcgcgtggg
    15661 atgagccgtt gcgtgctggg aactgatcgg actgatccga tcgctgctcc gccctgttcg
    15721 gcttgcatca ggacatccaa agctgtgtat cgcaattgca gcgtaagagc ttttgaattc
    15781 gaaatggata aaacgctcaa ggataagctg gcagggttgt cgctggggca attgatgaag
    15841 gttaaattgg atggcatgcc cgttggaagt ctgattttgc cctccatgcg ttggatcctg
    15901 cgcaaacatc atttggaaga tgatgaagat acacgccagt tagcccggca ctatattctt
    15961 tcggcagaaa atattatgcg cgaattcaac ctcctgctag atcaagccca accccaggca
    16021 gtggtcgtat tcaacgggat gttctaccct gaagcggtgg cgcgcttttt agctcaaaag
    16081 cgtggcattc cggtgtatac gcatgaagta ggcatgttgc ccttatctgc ctttttcacc
    16141 agtggcgaag cgaccgccta tccgatcgaa attccggatt cttttcggtt gtccaggaaa
    16201 caggacgggc aattggatgc ttatctcacg cagcgcacgg agggaaagtt cactacggcg
    16261 ggcatagaat tttggccgga gatgcgggca ttggatgctg cgttcgcgcg cgagatgcag
    16321 aaatttaaaa ccgtcgtatc cattttcacc aatgtgatct ttgataccag ccagacgcac
    16381 gccaatgtgg tctttccgca tatgttcgcc tggcttgaag tggtcctgaa atctatccgt
    16441 gcccatcctg aggtgctttt cattattcgt gcgcaccccg atgagctgcg cgcaggcaag
    16501 gaaagcgctg aaacagtagc cgattggatc agggacaacc acgtgctcga tttacccaat
    16561 gtgcgcttta tcaatgcgcg cgactacatc agttcttacg atttgatcaa cgcctccaaa
    16621 ttcatcatgg tctacaattc cactattggg ttggaggcat ctattttggg gaaacctgtc
    16681 ttatgcgccg ggaaagcccg ctacacgcag gttccgacag tgttcttccc aaaaacgaaa
    16741 gatgccttcc atgccatgct tgaagacttt ctgaccaacg atcctattca cgttcccgaa
    16801 gaatttcaat cgaatgcaag gaaagtcctt tatagtcaac tcttcatggc atcgctgcct
    16861 tttgaaactt ttctgaaaga agagaaacac tggagaggat acgtaggggt gcggaatttt
    16921 aagatcgatg cactcaaagc tgaaaactca cctaccatca gaacgattct gcatgggatt
    16981 caaaaaaagg agcaattttt acgcaaacca tgaacccgct ttgttcgatc gtcatccgcg
    17041 cattcaacga agaaaagcac attggaaaac tcctgcaggg aatttcttct caatctgttg
    17101 aatctgtgca ggttattctg gtcgattccg gttcaacaga tcatacggta gctattgcgg
    17161 agtcattcgg cgcaaagatc gtccatatcc aacccggtca gttcacgttc ggaaggtcat
    17221 tgaatttggg gattgcacag gcgaaggcgg attttgtggt gcttgccagt gcgcacgtct
    17281 acccggttta tccggattgg ctggaaaaaa tgctggaacc gttccgtgat gacaaagtag
    17341 ctctggtgta tggaaaacaa tctggctgtg cgacatccaa attctctgaa ctgcagattt
    17401 ttcgttgttg gtatccggat acttcgaact tcgatcaatc cacgccgctc tgtaataatg
    17461 ccaatgctgc catacgaaag tcgctatggg aaaaacaccc ttatgatgaa gagttgacag
    17521 gtctcgaaga cctggcttgg gcaacctggg cgaaagagca ggggcataag attgcttaca
    17581 gcgctgaggc ggagatcgtg cacgtccatc aagaaacatg gaaaggcatt tataaccgct
    17641 accgacgtga ggggatggct ttcaagcgca ttcacccaca agcaaccttc acgttcatgg
    17701 agttcatacg agtgctctgc cagaatatct ggaatgattg gcgagaagcc cgcatgcaaa
    17761 gaagattgcg gaaagagttc tgccgcattg ttcagttccg cttctgtcag ttctggggaa
    17821 cctatcaagg ttatcgacaa tccggaccgt taacctggca gttgaagcgt tcattttatt
    17881 acccaagaat tgaaccggag gtggataata gaaatggaag gcagggcgtt caacccattt
    17941 cctacggaaa agacaccacc ccggaaaaat caaaggagag tgagcgatga aatttgaaaa
    18001 gattgtggcg ctggttccca tgcgccatca ttctcagcgg gttcctcaaa agaattttcg
    18061 tgatctggct ggcaaaccct tgtacatgca catcctcaca tcgctgacca agtgcaaact
    18121 catcaaccag atcgtggtgg atacggatag ccccacgata aaagaaggga ttgctgaaaa
    18181 ctaccctcaa attaccgttc tcgagcgacc ggaacacctc cgcgccgaca cggtgccgat
    18241 gaacgagatt ctcttctacg acaccgaaca gatagctgcg gatctttacc tgcaaaccca
    18301 cagcaccaat ccgctattgt tgacagaaac gatcaccaaa gccatccaag atttttccga
    18361 cgcttatccg gcttttgatt ccctgttttc tgtgacgcga tggcagactc gtttatggga
    18421 cgggctggca agacctatca accacaaccc cgccattctg ctacaaactc aggacctccc
    18481 cccggtgtat gaggaaaatt cgtgcatgta tctcttcaca agagaagggt tgctgagaaa
    18541 gagaacccgc attggagacc gcccctacat gttcgaggtg tcggcgctcg aagcccaaga
    18601 tattgacgaa gagcaagatt tcttgatcgc tgacttgttg atgaaatatc gtctgcgcaa
    18661 tgagttgtta taattcctga aagacacaaa ggaaagaaaa atgcctaccg ttttgctttc
    18721 agcgccttat atgcttccat cggtggaacg tttcaaaccg gtatttaatc attatgggat
    18781 tgaattggtt attccagaag tccgtgagag gatggaagaa gagcagatat tggagtatgc
    18841 cgggcagttc gatgggacga tctgcggtga tgaccgttat tcggaaaagg tgatccagaa
    18901 gtgcctgccc cgcctgaagg tgatctccaa gtggggtacc ggtatcgacg ccattaataa
    18961 gccctatgct gaaagcatgg gagtgatggt cggcaacact cccaatgcgt tcacattgcc
    19021 cgtttccgat tcggtcatgg cttacctgct tgcctttgcc cgcaagcaac cctggatgga
    19081 tcgagctatg aaagacggga tatgggagaa gatccccggg cgttcgttga gcgaatgtgt
    19141 cctgggtgtg atcggcgtgg ggaacatcgg caaagcggtc atccggcgag caaaagcctt
    19201 tggcatgcgg attcttggaa atgatatcca acctattgcc ccggatttta ttcttgaaaa
    19261 tcatatagaa atgctgctgc ttgatgaact gctgacgcag gcggatttca tcagcctgaa
    19321 ttgcgacctc aacccaacca gttttcacat catcaatgcg cattccctgg aattcgttaa
    19381 gccagaggcg gtcctcatca acactgccag aggacctttg gtggatgaac cggctttgat
    19441 tgcagcatta cagggtgagc gtttgggcgg cgctgcgttg gatgttttcg aaatcgaacc
    19501 gctgccgtca gattcgccct tgcggaaaat ggaaaacgtg atgctggcag cgcataattc
    19561 caactccagc ccgtccgcct gggaacgtgt gcattggaac accatccgca atttgttgat
    19621 cggtttgcat atcgagcatg cagacctgga agatttacag cacaaatatg cgcttcaata
    19681 gggaaggagt gagatggcga aagaaaaggt cgtgttgatc accggcgctg ccggtggaat
    19741 tggcagcgca acggttatga aatttgcgcg atcggggtgg gacgtgatcg gtgtggatcg
    19801 cagggaattt gccggcaggt ttccttctga cgggttattt attctggcgg atatctccaa
    19861 gccaaaagat atatcattga tctatgaaaa gaccagtgat ttttctgcgc atttgaatgc
    19921 ggtggtcaat aatgcagcga tccaggtttc aaagccgatc ctggacacta ccatcgatga
    19981 atgggatctg gtgatcgctt ccaatttgcg ttcagcattt gtgggcgcca agttcgccca
    20041 tcccttgttg gtcaaagccg ggggaggggc gattgtcaat gtatcttccg tgcacgcggt
    20101 ggcgacttcg gtaaatgtgg cagcttatgc tgccagcaaa ggcgggttgc tggcactgac
    20161 gcgggcgatg gcgattgaat ttgcgaaaga tggaatcagg gtaaacgcgg tgcttcccgg
    20221 ggcggtggat acgcccatgc tgcgttctgg cttagatcgc gggcagcatg ggggcagcaa
    20281 tttggatgaa cgccttgaaa atctggcgcg caagaccgtg aatgggcgcg ttgggaagcc
    20341 ggaagagatt gcgaatacca ttttctttct ggcaacggaa gaatctgcct ttatgaccgg
    20401 gcaggcgctg gttgtggatg gcggagccac cgccaggttg agcacggagt agggcatgcg
    20461 cgaggagatc aaaaaacggg tacctgaagg attgttgaat atcggacggg ctacgcgcga
    20521 ttggatcgac cgttttccgg aagatgtttc cgcctgcacg catccctggc gcctggattc
    20581 acagcgcaag ctgcgaaaat atcacaaccg ctatcaaggc aagcgctgtt ttgtcattgg
    20641 caatggaccc agcttaaaga atacagacct tactgcatta tccaatgaat atacctttgg
    20701 aatgaaccgc atttatctca tgtttccgga acttggtttt ccgacttcct tcttggtatg
    20761 tgtcaatgac cttgttattg agcagaccgt ggaagatatg cagaagctgg cggtaccgcg
    20821 cttcttttcc tggcgctccc gcgcatggct gaggccagcc gatgacctgc actttctgca
    20881 caccacttac accggacctc gctttgcaaa aaatgctgca ggtcgtttgt gggaaggggc
    20941 gaccgtaaca tatgtctgcc tgcaactggc gtattatttc ggcttcacgg aagtaatttt
    21001 gatcggtgtg gatcacaact ttgttaccca aggcaaaccc aataccaccg ttgtatcgca
    21061 aggggatgat ccgaatcatt ttcacccggc gtattttggc aagggctttc gctggcagct
    21121 ccctgatttg gagacctcgg agctcgccta tcgtatggca aagcaagctt atgaagcaga
    21181 tggcagacgc atcgttgacg cgaccgtggg tggaaaattg accgtgttcc cgaaagttca
    21241 gtattccgat ctcttcaaag gttgatgtat gcgactgctg gtttccatcg tgacgccttc
    21301 gtataaccag ggagccttcc tagaagccac cattacttca gtgcttgacc aggattatga
    21361 agccctggaa tatatcctgg ttgatggaaa atcaaccgat ggcagtgtgg atatcattaa
    21421 aaaatattca gaccggcttg cctggtgggt ttccgaaaaa gaccaggggc aggcggatgc
    21481 catcaataag ggcttgagcc gcgtaagagg agagatcatc ggctggctca attcggacga
    21541 tgtctattta cccggcgcct tacaaacagt cgcgaccttt tttcaagacc accctgatgt
    21601 gggtttggtt ttcggcgatc tgctggctgt ggatgaaaat ggcgagcaga tcaatatctt
    21661 gcgctatgcg gattatacgt tgcaggacct gatggctttt cgtatcattg ggcagccggc
    21721 ggtgttcttc cgaaaagagg tcctgaagga agccgggttt ctcgatctct cgtatcatta
    21781 tttgctcgat caccaactgt ggattcgtat ggcgagcttt acgaacatcc ggcatattcc
    21841 ccaggttttg gcgggtgccc gctttcacca gggagcgaaa aatgtggctg cggctgaaca
    21901 gtttggcgaa gaagcgtatc gcattgtgaa ctggatgcgc tcgtatgaaa aaaccagttc
    21961 gatcctgccg ggaattgaaa agaaagttct ggcgggggca tgcctgatcg atggacgcta
    22021 cctgctggat ggagggaaac cccggcaggc gttcaaggtg tacgcgcgta gtctcttctt
    22081 acaccctgcc accggttttc gtgagtttca ccgcatgctc tatgcgttgg cggcaaccct
    22141 gggattggtt cgtgtgcaaa gtgtggtgga acggcgacga aaactgaata caataaaagc
    22201 aagattccat agaaagcaat gatctcatgc agcgcaagtc gtctatgaaa aacaaaagtt
    22261 ttttcgttct ggctttcctc ttgatcatcc ttggattggc gattcgcctc tttgacctcg
    22321 atgatgctcc tcttgatttt catcccaccc gccagcttca ctcggcgttg atggcgcgtg
    22381 gcatgtatta ccaattgacc ggcgatgcca gtgagaaggc tgctgtggcg atcagacagt
    22441 ggaaattgga aggattgatc gaacccccta ttatggaatg gttggcagcc tttggctaca
    22501 aattagctgg cggggtcgac ctgcgcattc cccgcctgat tgccatcctg ttttggttgc
    22561 tggcggtcat tccgctgctg gtcgttgcaa agcgcggagc aggttggaaa gctgctttgg
    22621 tgggggctgg cttttttctt tttattccgt atgggatcat tgccagccgt tctttccaac
    22681 cggaggcact gttggttttg ctgctggctc tctttttcat ggccctgtct ctctgggata
    22741 acgatcgatc ctggaaatgg gcagtatggg caggggttct gggtggtctg tctgtgctga
    22801 tcaaaaccgt tgccgccttc tttgtggcag gtatgtggct gggctggttg ctttcggatg
    22861 aaaattggaa aaccgcaatc aaagagaaga agatgtgggt ggcttttcta ttgaccgtgc
    22921 tgccctatgc cgcctatctg gcttacggat tgtggatcga taaaagttat gccggacagt
    22981 tcagtttgcg cttttttcct tccatgtgga cggagctatc cttctacctg cgctggttgt
    23041 ccaatttgcg gcgggttgtg gatgtgggtt ggttggtctt gggcatcata ggaacacttc
    23101 tgcttgaaaa gaaatccttg cggaatatcc tggtgggggc ttggatgggg tatatcctgc
    23161 tgggctttgt gctgccctat catatttcca cccatgatta ttatcatctc ccccttttat
    23221 tcctggttgc tttggggttg tgtggattgt ttgcaggcat tttttctctg ctcgaaaaaa
    23281 gatctgctgt gttaaaaggc atgggggggg tatgcttgct cttgttattc ggcgtttatg
    23341 cgatggacgc gcgttcggaa ctgaagaagg tgaattacag cgctgaagtg agcttttggg
    23401 aaagggttgc aaccctgttt tctcctgccg acagggtgat agctctcagt caagattatg
    23461 gcaaccgcct ggcttactgg gggtggctga ccccgcgcaa ctggcccagt ttggatgata
    23521 ttgaattgcg ccgggaaacc gggcaagacc tgaccctgga agattatttt gcggattaca
    23581 cggatggcta tgattatttc ttgataactg actttgatga gtttgcccgc caaccacaat
    23641 tggaggaatg gctgcacgat aagtatccgc tcctatttac ggatgagggt gttttattat
    23701 ttgatctgac ccctggaagt ccatgaggag atagataagt atgcagtctg acaaaaacac
    23761 caatccgcaa atatccgttg tgatccctgc ctataatgag caaaactgga tagaacttac
    23821 tctgcaatcg ttggtttctt ttttaggaaa ggaatttaat ggctgggaga tcatcgtggt
    23881 ggatgatggg tcagaagatg acacccagaa gatctgtgaa cggttctctc aagaaatagc
    23941 tcaggttgtc tatttgcgca atgcaaagaa ctctggaaaa ggtttttccg tgcgcagggg
    24001 gatgcaggca gccaggggtg aaaaacgctt tttcatggat gcggatatgc ctttcgagct
    24061 ggatgtgttg ttaaccatgt acaatgctct tgcaacagaa tgcgatatca gcataggtgc
    24121 cagggacctt cctggttcca ccttgatcgg cgtgccattc acacgttttt tggccgggca
    24181 gatcttcagc ttgctggtgc gcctctttgc ggtaaaaggt atcagcgata cgcagtgcgg
    24241 cttgaaaggg ttcacggcgc aggctgccga agccatcttc tcgcgcacca ccatcaacga
    24301 ctttggcatg gatgcggagg tgcttttcat tgcccagcat ttagattacc gaatccagcg
    24361 tgttccggtg cgcatgacgg gttttcgtgg caatagccgc gtgcatcttc taaaagattc
    24421 ggttaacatg ttttttgaat tgcttctcat ccgctggaaa gcgttgcgcg gggtttaccg
    24481 tttacgatga tctgatcgtt ctgagataga ctggcgattc gtcaatggta aaatcaaatt
    24541 tcacaaaggg aaaaatggat gatgcgtcgt tttcaatgcc cgcttgacaa gcaaaaaaca
    24601 gaacggcttt ttcgatctgt gttagtggtc gccaatcttt tgctctttat tgcactggct
    24661 gtatatgcct ggcggggttt ttattcgcgc tattgggcag atgattactg ctttggaagc
    24721 atttcccatg ctcatggttt gctcagtggc accaggtact tttaccaaaa cgtctccaac
    24781 cgttttgctg cttatttttt ggtgggtttg aatgagctgt ttggagaaag tgccatccgt
    24841 ttccttcctg cattcatgat catggtcacc gttgtaattt atacgaaagt atttcaattg
    24901 gcttgtcaga tcgtccgatt tccacagccc tggtatgttt cgctttttct cagccagacg
    24961 ttaacctatt tcatggtgct catggctcct aacctttttc aaagcctgtt ctggcgctca
    25021 ggaatggtca gttattttgc ccccatcccc ttctttggat tgagtctgat cctccttgta
    25081 tccggattga tgaagggttt tccatggtac aggctggatg gattatggtt gattgctttc
    25141 ttttctgccg ggctctcaga aacctttgcc gccatggaaa cgggggtatg ggtgatcttg
    25201 accctgggtg gttttctctt tctttcaaga aaagaacggc aaccgctgat gaaaggcgcc
    25261 ctgcctgcat tgatcggttc gctggcagcc atgggtatga tgatcctggc tcccggtaat
    25321 agcatgcgtc tggcaacgct ggaacaagcc agcaacccat ggcaggtgat cgttttgtca
    25381 ttgagatttg ctgccagttt ttcgtaccat actcttgtgg ggttgcccat tcctcttctc
    25441 gtcctgttcg ctgtggttct catagttgcc taccatctca gcgggcatac ccaaccagct
    25501 ttacaggatg cgcagtggat gaagctgttc tggggcagtt tggttgcggg attcatcttg
    25561 ttggtttgtg tggctgctcc cacagcatat ggcatgctgg cttaccctga agagcgtgcc
    25621 tggatgctgg gacggtttgt cactgtgctt gttctcatcg ttgctgcgtt ctcagcaggc
    25681 gtgctgagcc accgctggat ggatcgtatt gtggatacct gtttgatgtc catcattgtt
    25741 ctatttttgt taagtctcta cccactcaag ggagcgtggt ccgaattgca gcaacttccg
    25801 caatgggaaa cggttgctca tgcctgggat gtgcgccatg cacaaatcaa agaaaaggtg
    25861 gtcgctggtg aaactatctt taccgtacaa gctttcgatt ccatcggaac ggtggctgaa
    25921 ctaacagcga atccggatta ctgggtgaac gtgtgcgcag ccgattatta cggcgtggaa
    25981 gaaattgctg ccgtggaggg gttctatgag tgatcttccg cttgtttcta ttgttacacc
    26041 ctcctataac caggcgccgt atctggaaaa gaccattatt tcagtgttgg aacaggacta
    26101 tccgaatttg gaatattcga tcgtagatgg cggctccacg gatggcagcc tggacctcat
    26161 caagaaatat gccagccgct tgggttggtg gatttcggaa cctgaccagg ggcaaaccgc
    26221 agcaatcaat aaggggtttt cccatgctac aggaaagatc tatgcctggt tgaattctga
    26281 tgatacgtat caacctggtg cggtccgtga agcggtggat ttcttgctgc gcaatccaca
    26341 ggcggtcatg gtatatggag atgcacacta tattgatgaa agggataggg tgatcggaaa
    26401 ttttccggct gcccaaaccg acctgaaaag gttgcgccgt ggctatgttc acattccaca
    26461 gcaagcttca ttctttaccg cggaaggatg gaaagcggta gccccgctgg atgaatcgtt
    26521 ttattttgcc atggattatg acttgtgggt gcgcctcgcc aggttgggtc cgatcgtcta
    26581 tcacccgcgt gtatgggcaa atttccgctt gcatcaggat gcgaaaacga ttgccgccga
    26641 tgagcactgc tggcctgaaa tgctgcgtgt acattatcgg gacggaggtt cccactttgc
    26701 acccattgta tggaaatcct ggttgcgcaa attggctgcc ccctatattc gctggaaacg
    26761 aaaaaaaatg tttatttaat gaaggattga aaaatgcgcg acataaattt tttcaaagat
    26821 cttgtcttca aatttcgcct gtctgtcttg ctgatgcttg ataaacgggt agccgtgtat
    26881 ctgaaattga taccgatagc ggcattgatc tatttggttg ttccttttga tttggtaatt
    26941 ggtcctattg atgatgcggc attggttttg ggagctatgc agctattcat ctccctgtgc
    27001 ccgaaggaac tggtagaaca gcattcgcaa ggtctggaaa agcgaaagga agcggcgaaa
    27061 accagctcga caaaattctc gtaggcggga ttctttcccg ccgatctgat gatagcacgt
    27121 atgaaaacgt gttcaacaaa attcctgtag ggcgggattc tttcccgccg atttgatgat
    27181 ggcacgtata gaaacatatt tgacaaaatt cctgtagggc gggattcaca tcccgccgat
    27241 ctgatgatgg cacgtataga aacatatttg acaaaattct cgtagggcgg gattctttcc
    27301 cgccgaccta tgatggcgca tatagaaatg cgccctacga aaaaaatgct gacaggtttc
    27361 cccctttttt tgttgaaaac atgttcaaca aaattctcgt agggcgggat tccttcccgc
    27421 cttttatttt ggcgcgtata gaaacatgct cgacaaaaat ctcgtagggc gggattcaca
    27481 tcccgccttt ttaattgctg tacgtataga aagagtcatt caaaatcaat gttttcaatt
    27541 gaaattggaa ttcctgtcct tccccaattc tccggatata aatcttgtga gacaaaatca
    27601 tggaaactcg aaagtttata cccattgggg tcattggaag caccatgctt tacaggattc
    27661 caatgaatat aatctaagtg ccttttcata tctgtttcat cacggataat gtgatcccaa
    27721 aatctctttt gccataatga agtgggattt gtgatgctaa aagcggattt atagttcaat
    27781 gtgtagtttc ttttaaagca atgaattact tgtgagaaat tcccattttc attttccatt
    27841 tttaggatcc aatgaaaatg atctggcata agaacatatg catagagatc aaatttatga
    27901 attattttta ctttttcaag tgttgtgaaa tagagttgaa tgtttttcag attattgaaa
    27961 tatggaaagc ggttttttgt tacaaccgta ataaaaatat atgaattagg gatgtaatat
    28021 cggtggtagt gcggcatagg ttaattatta ctcccttctt ttttggcgtg tatagaaaca
    28081 tgttcgacaa aattcccgta gggcgggatt ccttcccgcc gatctgatga tggcgcatat
    28141 agaaatgcgc cctacgaaaa aaatgatgac ggtttcccct ttttttgttg aaaacatgtt
    28201 cgacaaaatt cccgtaaggc gggattcttt cccgccgatt tgatgatggc gcatataaga
    28261 aatgcgccct aaattacacc taactctttt ccgacggtct tgaactcctc gagcccccga
    28321 tccaggtcat ctttgctgtg agcggcggag atcatcacgc ggatgcgcgc tttgccctgc
    28381 ggaacagtgg gataaccgat ggaggtggcg aagatgccac gctcgaacaa ccgtttgctg
    28441 aaatcttggg cgagctgtgc ttcacccagc atgacggggg ttatgggggt ctggctgttc
    28501 ccaatgtcga aacccatgcc gctcatttcc cttttgaagt acgccccatt tgcccaaagc
    28561 gtatccacca gttctgtaga ttcttcgagc a
//
LOCUS       DAOSDS010000020        27193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_818_length_27193_cov_3.905483, whole genome shotgun sequence.
ACCESSION   DAOSDS010000020 DAOSDS010000000
VERSION     DAOSDS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 27193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27193
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_818_length_27193_cov_3.905483"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLL88_04030"
     CDS             complement(<1..656)
                     /locus_tag="PLL88_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ00763.1"
                     /translation="MNKTLMKLGIDYVGDHGLALASKPVIRKFAVNKALKLSEEGYYA
                     NLERGIYTPGECEDRYATSQAIIATAERVLTSSHAIAPAYLKKLADIMLKSIVVEGGD
                     VQTQQAFKALHGRRPPGFLVISPTKACNLQCKGCYASSSEVSHERLKWEVFDRLLSEA
                     RDIFGIRFIVISGGEPFTYKDQGKDLLDMVAKHDDMFFMSYTNGLLIDDKKAARLAEL
                     G"
     gene            complement(691..936)
                     /locus_tag="PLL88_04035"
     CDS             complement(691..936)
                     /locus_tag="PLL88_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00764.1"
                     /translation="MGELSNYYQIYIQGHLGQNWSEWLQSFHIQHKENGITVLTGPIV
                     DQAALRGLLDHLFDLGICILSFRRLGFHPEGRIFFSL"
     gene            complement(1018..3714)
                     /locus_tag="PLL88_04040"
     CDS             complement(1018..3714)
                     /locus_tag="PLL88_04040"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOJ00765.1"
                     /translation="METEINLLHTKFYLPKAREQLVHRSRLTHLLEEGLQGRMVLLSA
                     PAGYGKTSLLADWTQEFHHPTAWLTLDENDNDTARFMEYFFHSFFNRGYIQLQQLLTE
                     KAALFSGNLRQNLDILLNCILSSSENIIVVLDDFHHIHSPVINESINYMVENLPANAH
                     FVISTRADPVVNLPNWRVRGYLTEIRRADLCFTLKESTEFFQSALGDIFTRKNVRALM
                     NKTEGWIAGMQLAASAIKSLDDTYSIDLFIESFKGTNRFILDYLLDEALKNQSEETRN
                     FLLFTSLLEHFSASLCNHVLERNDSQQMLDFLERNNLFIVPLDNERTWYRYHTLFADL
                     LQSQLEKLDKERKRLIHQSAANWFETNHYYNEAIEHNLMAENMDAAERLIQLNAASVL
                     NHSQFTVFSNWLNRLPKEKLYQNPTLCTYYALTLLLRGNSYHEIKQILESMQEFADAG
                     FYGPAFIRSLLAIIQGNPQEALQHLDLIKSNPPQEDEFLSGYLELIQALTLPVNINQT
                     IENLRKTYLRSNIAGNLVIAITSRSYLGDLYNFMGKLTEAQKNYQEALDLSTIDKNAY
                     LPIGSNVLLGLGEIAYKRNQLDEADRFLSAGLEIKENIAISHFWGLCTSKARVCIAQG
                     RIQQALAYMQQAQELAIQFDTTEFDDFVVSCRIAQLHLLMGNLDAAEERVNALSMHDI
                     EKSRDLASFSIIYTPIQYLYPSTLAWCAIHQGKLSESIEILSKLYHESVEKNLGDFVL
                     QFSVLLAVAHEKNNAREEALRYLHKALDLAYPEGQVQIFLEQKENILTLLYEAAHRNI
                     HPEFTGHLLSLFPQMNTQEETDKFIIHDGEIIEPLSDREIEILALISQGFSNQQIAVQ
                     FHLSLSTVKVHTYNIYRKLQVHSRLQAVSKATLLKILPPTKK"
     gene            complement(3941..4720)
                     /locus_tag="PLL88_04045"
     CDS             complement(3941..4720)
                     /locus_tag="PLL88_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOJ00766.1"
                     /translation="MKRKRSVSVLSLLLCFVCFGINTHRSKDGMEMIYIPAGKSWQGS
                     GEWDHECKLNETPEHKVTVDAFWIDRTEVTNGMYAQCVDDGVCNLPSSREVNPRFYDA
                     SYVNYPVVYVTWQGAMNYCSWKGGRLPTEAEWEKAARGTTRRKYPWSEGYPTPENVNA
                     ANSVGDTTPVGSYLAGASFYGVLDLAGNVREWVYDWYSEDYYASAPRENPMGPDTGTD
                     KVLKGGSYYDNYEHTRIADRLFHPLDSGGINRGFRCVIPDL"
     gene            4907..7321
                     /locus_tag="PLL88_04050"
     CDS             4907..7321
                     /locus_tag="PLL88_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ00767.1"
                     /translation="MDIDNLISKMTRKEKAALCSGLDNWHTKPIERLNLPSSMMADGP
                     HGLRKEYPSNESQSFVPSYPATCFPTASALASSWNRDLLFEIGEALAEECLQAGVSII
                     LGPGVNIKRSPLCGRNFEYFSEDPFLSGEMAVNHIKGVQSKGIGTSLKHFAVNNQEFR
                     RMAINAVVDERALREIYLPAFEKAVKEAHPWTVMCAYNRLNGAYCSEHEQLLNDILRQ
                     EWGFEGLVISDWGAVNQRVDGLQAGLDLEMPGGKYENDEKIAHAIEKGELEEAILERS
                     ARRVLDLVFKSVQNRKDGFQYDEDRHHALARRAAAECIVLLKNEEKILPLAPSRKIAV
                     IGDFAKKPRYQGYGSLVINPTRLDNAFDEMVRYINDSGSIRYEDGFVRAEATVNPEME
                     SCACQAAGQADVVVVFLGLPELYETEGIDRDDMRLPDNQNHLMQELAKVTSNIVVVLS
                     NGSPVEMPWVNDAKGIVEAYLGGQAGGGAIADVLFGKVNPSGKLAETFPLKLEDNPSY
                     DNFPGGPQTVEYRESIYVGYRYYDKVNSEVLFPFGYGLSYTEFTYDDLQINPTVMNPG
                     ATVEISCTVSNCGERAGKEVVQVYVGRSDTSGFNPVRELRDFKKISLQPAETQKVTFY
                     LNKRAFAYYDTRKKDWQVACGEYQICIGTSSRDLRLVGRLVVDQREDPATTEIPLPET
                     VYAAIQDNHFEQAAFQGLYGKSLPNNSKADHSKLTLNTPLIDFKSSFWGKLVFAIMHR
                     EFRKQAPAGENILHERMVEKIMLEAPLQNLTNLSNGALTEKTAIALVHLSNGEILSAI
                     KKLIKN"
     gene            7396..8301
                     /locus_tag="PLL88_04055"
     CDS             7396..8301
                     /locus_tag="PLL88_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ00768.1"
                     /translation="MSALIGGGYAILAAILWAFSSFMLETIAKAIPPKELNVIKGAIA
                     TLLLTATSLLLGEDFRNIPWSDIFVLCLSGIIGISLGDTAYYAGLKDIGSRRALLLFA
                     LAPPMTALIAWIFLGEELSWTAWLGIFITVGGVGWVVTERTPQDTEERDTKTSHRGIL
                     MGVLASLGQAVGLVLSRSAMSEGVITPLQSAAVRLSAGVLFTLIWVGASRQGLGRWQN
                     STDRKNIWKLLGLTAFIGTYICLWLQQLAVKSAPAGITQTLLSTSPIFILPMAAMRGE
                     KLSWRAVLGAVISIFGIMLVFGLIH"
     gene            complement(8361..9398)
                     /locus_tag="PLL88_04060"
     CDS             complement(8361..9398)
                     /locus_tag="PLL88_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ00769.1"
                     /translation="MKIYISGISGFLSINLVRYLLGKGHKDIAGIDLVDFTYPERDNI
                     AFTQGDIRDKEAVRRSMQGADIIIHTAAALPLYTPEDIYTTDVVGTRNLLEQAQAYEV
                     KRFIHISSTAVYGVPDHHPLYETDPVWGVGPYGEAKVQAEAVCEEFRKKGLCVPIIRP
                     KSFVGPERLGVFAIFYEWASEGKNFPNIGSGRNRYQLLDVEDLCAAIYACMTLPEKQV
                     NDTFNIGAAEFTTMREDYQAVLDEAGFGKRIIPFPAKPVVAALKILEALKLSPLYPWI
                     YETASKDSFVSIEKAQRQIGFSPKYSNKDAMIRNYRWYLANKDTFKGAEGVSHRVPWK
                     QKALKWAKVFF"
     gene            complement(9494..10123)
                     /locus_tag="PLL88_04065"
     CDS             complement(9494..10123)
                     /locus_tag="PLL88_04065"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HOJ00770.1"
                     /translation="MFNKFFPQLGLAAVIFYVLAVLVGGAITPGYNHIYNTISELSIP
                     GQPDRWIINSLFILYNLSLFLFAAGGFQFVHLRQQKIARLVFILVMVSAVLGIAFIIF
                     PQDVRGAEATTAGTIHIILAGLTSPITIVCTILMGILLIREKNLHTWGIAFVVLSILI
                     LISGAATAYGVATNSMYGGLLERTTIGIYLLWIAILSIMIIKNKAILLD"
     gene            complement(10151..10225)
                     /locus_tag="PLL88_04070"
     tRNA            complement(10151..10225)
                     /locus_tag="PLL88_04070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10190..10192),aa:Arg,seq:ccg)
     gene            10328..11044
                     /locus_tag="PLL88_04075"
     CDS             10328..11044
                     /locus_tag="PLL88_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ00771.1"
                     /translation="MLLFYIRHAQSVNNALYDTTGSYEKRNEDAPLSETGKKQLPYLA
                     AFMQKEIDKVQADGQGGLRNVPVTLYCSLMERAVATASAVATGCQLSLYGYKDLHEVG
                     GVYLIDHASGEHIGMPGKGKAFLEEKYPLLRFPDDTREDGWWNRAFEEEKERYGRAGR
                     VLEALFTNHNQPDEVVIFVSHAEFFNYLIKRILSIPDAFPVWFELMNCAVTLFDFRED
                     HIRIPFLNRHHFIPNELITG"
     gene            complement(11259..12818)
                     /locus_tag="PLL88_04080"
     CDS             complement(11259..12818)
                     /locus_tag="PLL88_04080"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOJ00772.1"
                     /translation="MAGELLLTIDNGTQSVRALVFDLQGNMIAKSKVEIIPYVSPQNG
                     WAEQDPEYYWKKLCEACQKLWKENEIDKKSIAGLAVTTQRCTVINLDQNGKPLRPAMV
                     WLDQRRTSGLEPVRGLWGFLFKAAGMEDTVRYLQSEAEVNWIRTYQPEIWKQTHKFLF
                     LSGYLTYKFTGLYRDSVGSQVGYLPFDYKKQTWCAKSDWKWQAVPIEKEKLVDLVKPA
                     ELLGEVSRQASLETGIPEGLPVIAAAADKACEVIGSGCLDPHIGCLSFGTTATINTTH
                     SKYTEVISLIPPYPSAIPGAYSLEIQIYRGFWMVSWFKNEFADNEQRIAKNRGVQVEE
                     LFDDLIASVPPGSLGLTLQPYWSPGLKQPGPEAKGAIIGFGDVHTRAHVYRSILEGLG
                     YSLREGAERISRRSKIPITEVRVAGGGSQSKFATQLTADIFGLPAAKPHTYEASALGA
                     AIDLAVGLKLHPDFATAVSSMTRIGETFEPNAQNHRIYDELYQRVYCKMYKLLKPLYE
                     EIRDITGYPAD"
     gene            complement(12863..14440)
                     /locus_tag="PLL88_04085"
     CDS             complement(12863..14440)
                     /locus_tag="PLL88_04085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HOJ00773.1"
                     /translation="MINSNQILERSDIANQIWDALIIGGGITGAGIFRNLANEGYRVL
                     LIDQNDFSFGTSSRSSKMVHGGLRYLANAQFQVTYESVRERENLQKQYPNLVRPLQFL
                     LPIYDHYKYSASFFKAGIGIYDLFGSKWNHGSYTSAQIAANFSGLNHSGLNAVLYYYD
                     AEVDDSRLVYRVLNEGVMAGGMAFNYMQCTGFLQDASRKIEGVQIRDMLSGKESELHA
                     HAIVNATGPWTDDLRVKLHTQKAIRKLRGSHIIFKQERAPVKYAFTIFHPSDGRALFV
                     IPWQGTTMVGTTDLDHPEEYEREKPEPFMTGEEMDYLLEAANRLFPNLHLDERDIVSS
                     FAGLRPIISMGGMDPSKASRTHRIFKDENVFSIAGGKLTTFQRMAEDLIRQIRPSLPP
                     QKENHQSVSPTEEGSAKSLQDNAPQKRFSGIYGNLLPTFLSESKADEFQPLGSTPYTL
                     AEIRWAVRNEMVCHLSDLLLRRTRLGLLASEGGAGVLDAIKPIVKQELKWSENIWQKE
                     VQAYQRIWKDCYSLPSK"
     gene            complement(14451..16040)
                     /locus_tag="PLL88_04090"
     CDS             complement(14451..16040)
                     /locus_tag="PLL88_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOJ00774.1"
                     /translation="MKRWNGWGKTDYTYHFAESSKQFVERMVGKGEIIPDATLETVLK
                     KVPNSRLPENKLYTTDAEIRLRHARGQSFPDWVALRYGTVDTFPDAVAMPSRKDDFDT
                     LIDLAKKGNFHLIPYGGGTSVVGHINVENRDTPTLSVDLARYNQLFHLDEISHTATFG
                     AGVCGPDMEETLRQHGFRFGHYPQSHEFSTLGGWIASRSVGTQCYYYGRIEHLFLGGL
                     MHTPQGLLEMPPFSASAAGPDLRHCALGSEGRMGIISEATVRISPRPQFEHFYAVFYP
                     DWDSGFAALREIVQARLDVSMLRLLDKFETETTLQLSGKEKLVKLADQGLRLIGMGEE
                     RCMMLFGITGSPVVARTTLHQVQHIARKHHGFTEKRFIGKLWFDSRFTTPYLRNNLWE
                     CGFALDTLESVFPWSRVAAVKDEVEAALFNGLQDENERLIVFSHLSHMYTDGASLYVT
                     YLFRRTDNADQTLERWKKLKQAASDVIVKNHGTISHQHGVGIDHRPYLPLEKGALGMR
                     SIQNFYRTFDPDGIMNPGKLY"
     gene            complement(16037..16741)
                     /locus_tag="PLL88_04095"
     CDS             complement(16037..16741)
                     /locus_tag="PLL88_04095"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ00775.1"
                     /translation="MNWEPPEKPTDFTEKRLIEAILQGVYPVDSYLPSERELCAQLGV
                     TRPTLRETLQRMRRDGWVEIHQGKPTRVRNYLQEGNLNVLNALANANAQISLNIVESL
                     LETRLLLAPTYFTNASRLHPTDLTDFLIRIIPISNEPAQAGLLDWELHKKASFLSGNP
                     AFLLILNGFEQVYLKMAERYFDQADARAASEFFYRNVLQHTQEQAFDSVFRCTQKMMQ
                     DSIQFWRNSIATLEEL"
     gene            complement(16835..17809)
                     /locus_tag="PLL88_04100"
     CDS             complement(16835..17809)
                     /locus_tag="PLL88_04100"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HOJ00776.1"
                     /translation="MNDSPAGNDRRFHIGDIPVSPGLVVAPMDGITDSAFRTILCRQG
                     ATLCYTEFINARDVLFDSIFYTRKITRVRAEEHPIGFQIYDSDPLNILQAGKKLMQFH
                     PDFFDINIGCSVARVANRGAGAGLLLDPAKIAEIFDRMTDAFSIPITAKIRLGWNDRT
                     QNYLQISQLIQEHGGAMIAVHARTKEQGFEGKANWAAIRQIKDILKIPVIGNGNVTRV
                     KEIHEMRDLTGCDAVMVGRAALANPWLFSYRDREEVSNKEVFDLMQSHLGLLLEDYPV
                     GISLIFFRKFVNQYLKPLSLDRQQKIRLFSHTQPDELLKAVQGLLDLR"
     gene            complement(17845..18729)
                     /locus_tag="PLL88_04105"
     CDS             complement(17845..18729)
                     /locus_tag="PLL88_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOJ00777.1"
                     /translation="MNKFNEFLKSKKLLVSDGATGSSLQQHGLDKGKPAEIWLYENPD
                     AIQQLHLDFIRAGADILLTCTFGANALRLEHAGLSGKVKEANTLAVKIAQNAVADRAV
                     LVAGSIGPLGQMLKPLGLLAVDDARNIYAEQAQALCEAGVDLLVIETQFDIQEASAAI
                     QGARSMCDLPIICSFSYDRGVKTMMGVSPTQMVQTFSSFNLAAFGINCGKSLEDNLRC
                     LKELKQVTDKPIWFKPNAGLPTVDETGKINYSTTPADMGSQVKEWVAEGAVIIGGCCG
                     TSPAHLAAIAEKVKESGS"
     gene            complement(18744..19874)
                     /gene="chrA"
                     /locus_tag="PLL88_04110"
     CDS             complement(18744..19874)
                     /gene="chrA"
                     /locus_tag="PLL88_04110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HOJ00778.1"
                     /translation="MKANNKLYEVALLFMKLGIIGFGGPLANIALMEQETVHKRKWVN
                     QEDFLNMLAITNLIPGPNAVEMALHLGYVYAGNAGLWIAGGAFILPSFLFSLILGILY
                     SKFGNVPQVEAVFYAINPLLISVIITSAFHMGKEVLKDWKAATIFVVFMVVALLGLNE
                     TLVIILAGVVWVLLHLPHKPKEKANLLVLLPLISEGFLNLPRTSVPFIFLYFLKTGAV
                     LFGSGLALLALIQNDVVNTFHWLTQQQLIDAITVGQVTPGPVSSTATFIGYQIASFPG
                     AVVATVGIFLPSFLIVLLILKWIPNIKKGGIFHDFLQGVTIGVIALILVVAYRIFRSA
                     INDVFTLAIALIGLLLLWKEKVETIYLVLAGIAISLIKFYLF"
     gene            complement(19858..22788)
                     /locus_tag="PLL88_04115"
     CDS             complement(19858..22788)
                     /locus_tag="PLL88_04115"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOJ00779.1"
                     /translation="MGNETAVKFELNQSTWTGSADSNQNALDFLRGLNLKSIKSGCRV
                     GDCGVCTILLEGQPVRSCMLKVKDLEGKSVLTLEGLNQDGKMHPLQESFMETGAIQCG
                     FCTPAQILTAKAFLDKNPNPTEEEVRSSLKGVLCRCTGYVRIVEGVMRAAARMQGKKL
                     DDYGPLYLDLLEKFADIHIPEPYLRKDGGEHPLPPLVYTPPSMSKTDVVNQTPKKVDA
                     QKLVTGKPAFTDDFRPEGMLFGAILTSNVAHAHIRSINIEKAKALAGVRAVLTYQDVP
                     RVKYTSGGQSYPQPLPYDQVSLDHKVRHVGDRVAVVAADTQEIADKALQLIEVDYEML
                     EAVVDPREAIKDGAPIIHDEEDTEGIYDAKHNIVYHIEAQNGDPDKVFAEADHVVAGE
                     FHTPKQQHAQLEPHICITYFDPDDRLVVLSSTQVPYHVRRIISPLVGIPIKNIRVIKP
                     RIGGGFGGKQEILLEDLCALLTIKTGRPVRLEYSRRQEFTSGRTRHQHYIQYKVAVQD
                     EIVTAVELKLLADTGAYGTHGLTTSMVGGFKGLTLYNAFDSRFICDVLYTNTPTAGAY
                     RGYGTMQELFALEVLMEEIAEKLGVDVVEFKRKNWLKLGEPMHMAKKLGEGREGFDQS
                     MASSGLSQCVDIGLKATRYYEKRKEYKNQSGTIRKGIGMSIALHGSGIAGLDMAAATM
                     KMNDDGSFNLLIGATDIGTGSDTILAQIAAEELGVTVDDIIVYSSDTDFTPFDKGAYA
                     SSTTYISGGAVKKTAAAIKKQIQEQAALMLKWDDPEKLTLKDSRVVSPEGKSVTLQEV
                     ALSSLHQLNQHQIMATESHRSYVSPPPTAAQFAEVAVDTETGEIKVERLLMVVDCGRV
                     INPITAAGQVEGGMAQALGFAHCEETYIDEKGQILNPRFETYHTYLANEMPPTDVIFV
                     QTDEPSGPFGAKSIAEISIDGVAPALVNALHNASGVWLHDLPLTPEKVWKALHPEKRH
                     EGKQ"
     gene            complement(22788..23417)
                     /locus_tag="PLL88_04120"
     CDS             complement(22788..23417)
                     /locus_tag="PLL88_04120"
                     /inference="COORDINATES: protein motif:HMM:NF015415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00780.1"
                     /translation="MMKLTITSAETLEAIYSRKDLPPTLHRILEQNTFQERAETEFLV
                     SVRARNSFPDWGAGLNAINPTATLAAGQKTPFISLLEKGAGSLNELKEVQLDLDESDL
                     LAYEKVSNTPADRPIISVIIYLKFKERMIDQLRIAATGVSKAAYDCTDFSEDMSGKRL
                     DSVLMKAISEKFKEMITPVDTYMGSAVYRRDMAGVLVERILSKKINGDL"
     gene            23457..23621
                     /locus_tag="PLL88_04125"
     CDS             23457..23621
                     /locus_tag="PLL88_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00781.1"
                     /translation="MIVKLYLLIIQNCTVEKDEDIQHLTHPLAQLGEQQRELLHLRFS
                     ANYGILPLGF"
     gene            23737..24852
                     /locus_tag="PLL88_04130"
     CDS             23737..24852
                     /locus_tag="PLL88_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOJ00782.1"
                     /translation="MEKIILAFITGLTTGGLSCLAVQGGLLAGSLVNQVETDLAHTKN
                     KRKGTQQHDKAFHLAYPLLIFLLTKLISYTLAGFLLGLVGQMFQLSTFTRAILQIAVG
                     IFMLGNALRILNVHPIFRFFSFEPPAFFRRFVRKSATRQQTSSVLIPAFLGFLTVFMP
                     CGVTQAMMAAAIATADPIQAAVLMAAFTLGTSPVFFAVMYFSFQLGAKLERYFMKFVA
                     IVLLVFALITIDGGITLAGSPLSITRMVNNLTNKNEADTSLSPEFSSVPAGAALDETS
                     EDSAVLENGITITVKNNGYYPNAVHVKAGMPIQLTLVSENVNSCARAFVIPTLRIQKL
                     LDVTGEVVLEIPAQEEGTRIPFSCSMGMYTGSIIVDE"
     gene            24865..25074
                     /locus_tag="PLL88_04135"
     CDS             24865..25074
                     /locus_tag="PLL88_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOJ00783.1"
                     /translation="MQKIILKIEGMHCPNCALALERVEDTLPGIEKVDASYHKARMVV
                     EFDEHIVSEEAIRDEILKLGYGIVS"
     gene            complement(25181..26377)
                     /locus_tag="PLL88_04140"
     CDS             complement(25181..26377)
                     /locus_tag="PLL88_04140"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HOJ00784.1"
                     /translation="MPTNQTAEFPATWNQVVAFRLSRHHLLERAPANALAAVAGDMAG
                     VQAQLLSAAQISLWSRVRDLHTANIERALSERVLVKAACMRQTQFIVPSEQLAIFVRG
                     SARRAEKEIRWARGKGVPDRIIDAAIDAALGAALGALDQPRTRPEIAEHVSRILGVQA
                     QTIRGGGWGSRRKIAAVPVGEITFPVVDLLHLAAARGVVCSGPNRGNEPTFVRADAWI
                     PKWQDVAKEQAEVSLLRRYLRAFGPATATDFALWTGMTLTEAREIWAHEQYGFMPVTV
                     EGQAAEILREDLDVLAQAGFEQPLVRLLPYFDTFLLGHKERDHLVAREHQPHIYRAQG
                     WIAPVVLVGGRVVAVWEQVREGNHLRVKVTKFGSISPRIMSEIQEEAHNLSQFLGVPS
                     VDIQMD"
     gene            complement(26596..>27193)
                     /locus_tag="PLL88_04145"
     CDS             complement(26596..>27193)
                     /locus_tag="PLL88_04145"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOJ00785.1"
                     /translation="EIYQRITTHPFSRFPVFKDTPDNIVGIIQSRDLLLQRINGPDFD
                     LNKILIKPIFIPESTPALDVLDLLKASLTEIAIIVDEYGGVLGLVSINDIIEAIVGDM
                     MNPYGKFEAEIIQREDGSWLVDGMVLMDELKESISIDKLPNEEEGNYDTLSGLMMSQL
                     GKVPVSGDYFTWGNLRFEVVDMDGRRVDKVLIAPINNT"
BASE COUNT         6807 a         6448 c         6756 g         7182 t
ORIGIN      
        1 cccaactcgg ccaggcgagc ggcttttttg tcatcgatca aaagcccgtt ggtgtaggac
       61 atgaagaaca tgtcgtcgtg cttggcaacc atgtccagca ggtcttttcc ctgatctttg
      121 taggtgaagg gttcgccgcc gctgatcacg atgaagcgga tcccaaagat atcgcgcgct
      181 tcgctgagca gtctgtcaaa cacctcccat ttcaggcgct catgcgaaac ctctgaggaa
      241 ctggcatagc accccttgca ttgcaggttg caggctttgg tcgggctgat caccaggaac
      301 cctggcgggc ggcgtccgtg cagcgctttg aaggcttgtt gggtttgcac atccccgcct
      361 tcgaccacga tactcttgag catgatgtca gccagctttt tgaggtaagc gggagcgatg
      421 gcgtgcgagc tggtgagcac gcgttcagcc gtggcaatga tcgcctggct ggtggcatag
      481 cgatcttcgc attcgcctgg cgtgtagatc ccgcgctcca gattggcata atagccttcc
      541 tccgacagtt tcagggcttt gttgaccgca aatttccgga tcactggctt ggatgccagc
      601 gctaagccgt gatcccccac ataatcgatc cctagtttca taagtgtctt gttcatcgtc
      661 tttttcttct cctttcgtca gtctgttaca tcacaatgag aaaaagattc ttccctcggg
      721 gtgaaaaccc aaacgtctga atgagaggat gcaaataccc aggtcaaaca agtgatctag
      781 caatcctcgc aatgcagcct gatccacgat gggaccggtc agaacggtga tcccgttttc
      841 tttatgctgg atgtgaaatg attgcaacca ctctgaccaa ttctgaccga gatgaccctg
      901 gatgtagatt tgatagtaat tgctcagttc tcccattcaa acgcattcct gtattcttaa
      961 actcaagaat atcgcctgat ggcacgcact acaacgagca aaggtattat tcaggtatta
     1021 ttttttcgtt gggggtagga tcttcagtag agttgctttc gagaccgctt gtaaacggct
     1081 atgtacctgc agtttacggt agatgttata ggtatgaact tttacggtgg atagcgaaag
     1141 atggaactga acggcgatct gttggttgga gaatccctga cttatcagcg ccagaatttc
     1201 gatctcgcga tcgcttaatg gctcgataat ttctccatcg tgaatgataa acttgtccgt
     1261 ctcttcctgg gtattcattt gcggaaagag ggagagcaag tgaccggtga attcgggatg
     1321 gatattccga tgggctgctt catagagtag agtgagaatg ttttctttct gctcaagaaa
     1381 gatctgcacc tgcccttccg ggtatgccag atccagtgct ttgtggagat acctgagcgc
     1441 ttcctctcgg gcattgtttt tttcatgggc aacagccaac aaaacggaaa actgcaagac
     1501 aaaatccccc agatttttct cgaccgattc atgatagagt tttgatagaa tttcgatcga
     1561 ttcggacaat ttaccttgat gtatggcaca ccatgccagc gtagaagggt acagatattg
     1621 aattggagta tatatgatgg agaatgaagc cagatctcgg ctcttttcaa tgtcatgcat
     1681 cgaaagggcg tttacacgtt cttccgcagc atcgagattc cccatcaata gatgcaattg
     1741 ggcaattcgg caagaaacaa cgaaatcatc gaattcagtg gtatcaaact gaatagccag
     1801 ctcttgcgct tgttgcatat atgcaagggc ttgctgaatt ctcccctgag cgatgcaaac
     1861 gcgggctttt gaggtgcata atccccaaaa atgactgatt gcaatgttct ctttgatttc
     1921 cagtcctgcg cttagaaacc ggtctgcttc atcaagttga tttcttttat aagcgatctc
     1981 ccccaatccg agcagaacgt tgcttccgat cgggagatat gcatttttat caattgtgga
     2041 tagatcgagg gcttcctgat aatttttttg agcttcagtc agtttcccca taaagttgta
     2101 gagatcgcca agatacgatc gtgatgtgat ggcaatgacc aaatttccgg ctatattgga
     2161 acgcaaatat gtttttcgca gattttctat cgtttgatta atgtttaccg gcaaggttag
     2221 tgcctggatc agttcaagat aacctgataa gaattcatcc tcttgtggtg ggttcgattt
     2281 tattagatct aggtgttgaa gggcttcttg cggattcccc tggatgatag cgagcagtga
     2341 tcggatgaaa gctggtccat aaaatcctgc atcagcaaat tcttgcattg attcaagaat
     2401 ttgcttgatt tcgtgataag aattcccccg caatagcaat gtcaaagcgt aatatgtgca
     2461 aagagttgga ttttggtaca gtttttcttt gggcaaacgg ttcaaccagt tactaaatac
     2521 ggtgaattgg ctgtgattca gcacagaggc tgcattgagt tggatcaacc gttcagccgc
     2581 atccatgttt tcagccataa gattatgttc aatcgcctca ttgtagtaat gattagtttc
     2641 aaaccaattg gcagccgatt gatggatcag cctttttcgt tctttgtcca atttttccaa
     2701 ctggctttgc agaagatcag caaataaggt gtggtatctg taccaggtgc gttcattgtc
     2761 cagcggaaca ataaaaagat tgttccgttc taaaaaatcg agcatctgtt ggctgtcatt
     2821 tctctctaaa acgtgattgc ataaagaagc gctgaaatgt tccaataacg atgtgaagag
     2881 aaggaagttg cgtgtttctt ccgattgatt cttgagcgct tcgtccaata aataatccag
     2941 gatgaaacga ttggtgcctt taaatgattc aatgaaaaga tcaatgctgt aagtgtcatc
     3001 caacgatttg atggcagatg cagctagctg catgccggcg atccaccctt cggttttgtt
     3061 catcaatgca cgcacatttt tccgggtgaa gatatctccc agcgcacttt gaaagaattc
     3121 cgtagattct tttaatgtga agcagagatc cgctctgcgg atctctgtca ggtaacctcg
     3181 tacgcgccaa ttgggaaggt taacgaccgg atcagcccgc gtcgaaatca caaaatgcgc
     3241 attggctggt aagttttcaa ccatgtagtt gatcgattca ttaatgacgg gcgaatgaat
     3301 gtggtgaaaa tcgtccaaga caacaatgat attttctgaa gaagagagaa tgcaattcaa
     3361 tagaatgtca aggttttggc gaaggtttcc tgaaaacagc gctgcttttt ccgtgagcaa
     3421 ttgttgcaat tgaatatatc cccgattgaa aaacgaatgg aagaaatatt ccatgaaacg
     3481 agcggtgtca ttgtcatttt catccagtgt cagccaggcg gttggatgat ggaattcctg
     3541 ggtccaatca gccaataagc ttgtcttccc atatcctgca ggtgcagaaa gcaaaaccat
     3601 ccgaccttgc agcccttctt ccaaaaggtg cgttaaacgt gaccgatgaa caagctgttc
     3661 acgggctttg ggcaaataga actttgtatg taataaattg atttctgttt ccatttcttg
     3721 caagaagtgc ctttaaccca ttacgatgag tgtagcatat ttgcagaaaa tggagaacat
     3781 gctgcattgc gacgaatggt attttcacaa aaaacctgtg atgttaggca tcacaggttt
     3841 ttcaaagaaa tgcagcacgc attctgaaaa tttcaattta ggattgttta tgtgaactgg
     3901 agtgcattat acccgatttt atgggttgtt tccaaatcgt tcagaggtct ggaatgacgc
     3961 aacgaaaacc gcggtttata cctcctgagt caagcggatg gaatagtcgg tcagcgatgc
     4021 gcgtgtgctc atagttgtcg taataggaac cgcctttcaa taccttatcg gttccggtat
     4081 ctggacccat agggttctct cgaggagctg atgcgtaata gtcttcagaa taccaatcat
     4141 agacccattc tctgacattt cctgccagat ctagaacccc ataaaaactg gcgcctgcga
     4201 gatatgatcc tacaggggtg gtatctccaa cgctgttggc tgcattgacg ttttcaggtg
     4261 tggggtagcc ttcggaccag ggatatttcc tgcgggttgt accgcgcgct gctttttccc
     4321 actcggcttc agtgggcaac cgtccccctt tccagctaca gtaattcata gcgccctgcc
     4381 aggttacata aaccaccgga taattcacgt acgacgcatc gtaaaaacga gggttcactt
     4441 cgcggctgct tggcaggttg cacacgccat catccacgca ttgcgcatac atgccgtttg
     4501 tcacttccgt tctatcgatc caaaacgcat ctacggtaac tttgtgctcg ggcgtctcgt
     4561 tgagcttgca ttcatgatcc cattcccctg atccctgcca ggatttcccg gcagggatat
     4621 agatcatctc catcccatct ttggagcgat gggtgttgat tccgaagcaa acaaagcata
     4681 gcaacaatga taagaccgac actgaacgct ttcgtttcat gattccagaa ctcgcgaaaa
     4741 atgggatgtt gtttagcttt catgattcta tcccattctt gtctattcat ggggtgattg
     4801 ctgtatattt gtgccgcgtt cgtgacacga tgcatttata atatctacaa taaggggtac
     4861 gaaaacctta aatttggaaa aatggtgaag tgaggggatg tggtttatgg atatagacaa
     4921 tttgatttcc aaaatgacac gaaaggagaa agccgctctg tgttcagggt tggataactg
     4981 gcataccaaa cccatcgaac ggttgaatct cccttccagc atgatggcgg atggaccaca
     5041 tggactgcgt aaagaatacc cttccaatga aagccagtct tttgtcccca gttatccggc
     5101 gacctgtttc ccgactgcat ctgcgttagc gagcagttgg aatcgcgatc tgctttttga
     5161 gatcggagaa gcattggctg aagaatgcct gcaggcgggg gtcagcatca ttctgggtcc
     5221 gggcgtgaac atcaagcgct ctcctctgtg tggtagaaat tttgaatatt tttccgaaga
     5281 tccctttctc agtggagaaa tggcggtcaa tcacattaaa ggggtgcaaa gcaaagggat
     5341 cggaacttcg ctgaaacatt ttgctgtcaa caaccaggaa ttccggcgta tggcaatcaa
     5401 cgcggtggtg gatgaacgcg ctctgcgcga aatctatttg cccgcttttg aaaaagccgt
     5461 aaaagaagcc catccctgga cggtgatgtg tgcctataat cggttaaatg gagcgtattg
     5521 cagcgaacat gagcaactgc taaacgacat tctacgacag gagtggggtt ttgagggact
     5581 ggtcatatca gattgggggg cggtcaacca gcgggtggat ggcttgcagg ctggtttgga
     5641 cctggaaatg cccgggggaa aatatgaaaa tgatgaaaag atcgcgcatg caattgaaaa
     5701 aggagaatta gaagaagcga tcttggagag gagtgcacga cgcgtgctgg acctggtttt
     5761 caaaagcgtc cagaatagaa aagatggatt tcaatatgat gaagatcgcc atcacgcatt
     5821 agcgcgccgg gcagcagcag aatgtattgt gttattaaag aatgaagaaa aaatcttgcc
     5881 cctggctcct tcacggaaga tcgctgtgat cggtgatttc gcgaagaagc cacgctatca
     5941 aggttatgga agtttggtga tcaatcccac tcgcttggat aatgctttcg atgaaatggt
     6001 tcgatatatc aacgattcgg ggagcattcg atatgaagac ggttttgtaa gggcagaagc
     6061 gaccgtcaac ccagaaatgg aatcgtgcgc gtgccaagcg gctgggcagg ctgatgtagt
     6121 ggttgttttt ctcggtttgc cggaattgta cgaaactgaa ggtattgatc gtgacgatat
     6181 gcgtttgccc gataaccaga accatctgat gcaggagctg gcaaaagtaa cctctaatat
     6241 tgtggtggtg ctgagcaatg gctctccagt ggaaatgccg tgggtgaatg atgcgaaagg
     6301 aatcgtggaa gcctatttgg gcgggcaggc gggaggcggg gctattgcgg atgtgttgtt
     6361 tggcaaagtg aacccaagcg ggaaactggc agaaacgttc cccttgaaac tcgaagataa
     6421 cccttcttat gataattttc ccggaggacc gcagaccgtt gagtatcggg agagcattta
     6481 tgtgggatac cgctattacg acaaggttaa cagtgaagta ctgttcccct ttggatacgg
     6541 tttgagttat acggaattca catatgatga tctgcaaata aaccccacag tgatgaaccc
     6601 aggtgcaaca gtggagatca gttgtacggt aagcaattgt ggtgaacggg ctggcaaaga
     6661 ggtcgtgcaa gtctatgttg gtcgaagcga tacttctggg ttcaatcccg tgcgagagtt
     6721 gagagacttc aagaagatct cgctccaacc ggcagaaacc caaaaagtca ctttctacct
     6781 gaacaaacgg gcatttgcct attacgatac ccggaaaaaa gattggcagg tagcgtgtgg
     6841 cgagtatcag atttgcattg gaacatcatc cagagacttg cgactggtag ggaggttggt
     6901 agtggatcag agggaagatc ccgccacgac tgaaattcct ttgccagaaa ccgtctatgc
     6961 agcaatccag gataatcatt ttgagcaggc tgcctttcaa ggattgtatg gaaaatcgtt
     7021 gcccaataat tccaaggcgg atcattcgaa gcttaccctg aacacaccct taatcgattt
     7081 caaaagcagt ttttggggga aactggtgtt tgccatcatg cacagggagt tccgaaagca
     7141 ggctcccgca ggggaaaaca ttctgcatga gcgcatggtc gagaagatca tgttggaagc
     7201 accgttgcag aacctgacaa acctcagcaa tggggcatta acagaaaaaa cagctatagc
     7261 tcttgttcat ctgtccaatg gggagatcct gtctgctatc aagaaattga tcaaaaattg
     7321 acgctcgtga tgagcaacga gagtgatata ctcgctccat tattcattga tgagaacggg
     7381 agtaatcatt cgtccatgtc cgcattgatc ggtggaggat acgccattct ggcagccata
     7441 ttatgggcgt tttcctcatt catgcttgaa actatagcga aagccattcc acccaaagaa
     7501 ttgaacgtca tcaaaggcgc tattgccacc ctgttgttga ccgccacttc gttgttgtta
     7561 ggagaggatt tcaggaatat tccctggtct gatattttcg tgctttgttt gagcggcatc
     7621 atcgggatca gtctgggtga tacggcatat tatgcagggc tgaaagatat cggatcgcga
     7681 agagcactgc tgttgttcgc gctggcacca cccatgactg ctttgatcgc ctggatattt
     7741 ctgggcgaag aattgagttg gactgcatgg ttgggtattt ttatcaccgt gggaggagtt
     7801 ggctgggtgg tcacagagcg caccccgcag gatacggaag aacgcgatac gaaaacttca
     7861 cacagaggaa ttctgatggg tgtgctggct tctcttgggc aagcggtggg tctggtgctt
     7921 tcccgctcgg ccatgagcga gggggtgatc acaccactgc agagcgctgc ggtgcgatta
     7981 agcgcggggg ttctttttac cctcatctgg gtgggcgcca gccgtcaggg cttgggtagg
     8041 tggcaaaaca gcaccgaccg gaaaaatata tggaaattgc ttggcttgac tgcattcatc
     8101 ggcacgtata tctgcctgtg gctgcaacaa ctcgcggtga agagcgcccc agcgggaatt
     8161 acgcaaacgc tgctttctac cagccctatt ttcatcttgc caatggcagc catgcgaggt
     8221 gagaagctga gctggcgcgc tgtgttgggc gcagtaattt ccatctttgg aattatgctg
     8281 gtgttcggct tgattcacta gctatgtctc tggttctttg acgggcagca gatccggtaa
     8341 accggttttt caagaaacga ttaaaagaag acttttgccc acttgagagc cttctgtttc
     8401 cagggtaccc ggtggctcac accttctgct cctttgaacg tgtctttatt tgccagatac
     8461 cagcgataat tgcgaatcat ggcgtctttg ttggaatact ttgggctaaa cccgatctga
     8521 cgctgggctt tttcaatgga aacaaaagag tccttcgagg cggtttcata gatccagggg
     8581 tagagggggg agagcttcag cgcttccaga attttaagcg ctgcaaccac cggtttagca
     8641 gggaaaggta tgatccgttt gccaaaacct gcctcatcca acaccgcctg atagtcttca
     8701 cgcatggtgg tgaattcagc cgcaccgata ttgaaggtgt catttacctg tttttctggg
     8761 agggtcatgc aggcataaat ggcagcgcac aggtcttcca catccaacag ttgatagcgg
     8821 tttctgccgc taccgatgtt ggggaaattc tttccttcgg aagcccattc gtagaaaatg
     8881 gcaaatacac ccagccgttc aggaccgaca aaagatttgg gacggatgat gggtacgcat
     8941 aagcctttct tgcggaattc ttcgcatacc gcttcggctt gaaccttcgc ttcgccgtac
     9001 gggcccacac cccatacggg gtctgtctcg tacaggggat gatggtctgg cacgccatac
     9061 acagcggttg atgaaatatg gataaagcgc ttgacctcat atgcctgcgc ctgttccagc
     9121 agattgcgcg ttccaaccac gtcggtggtg tagatatctt caggggtgta aaggggaagc
     9181 gcagcagcgg tatggatgat aatatccgct ccctgcatgc ttctgcgtac agcttcttta
     9241 tcgcgaatat caccctgtgt gaacgcaatg ttgtcccgtt cgggataggt aaaatcgacc
     9301 aggtcgatac cggcgatatc cttatgccct ttacccaata aatagcgcac caaattgatg
     9361 ctcaagaacc cgctgatacc gctgatgtaa atcttcatat tccgcactcc taaccatgac
     9421 ttcggcttat tgaaacgtga ttatatcgta aacaggctga ccctttaaga accgggcgcc
     9481 aacccttaaa aaatcaatcg agcagaatgg ctttgttttt gatgatcatg atggaaagga
     9541 tggcgatcca tagcaagtaa atgccgatag tggttctttc tagcagaccg ccgtacatcg
     9601 agttggttgc cacaccgtag gcagttgctg caccgctgat cagtataagg atgctcaaaa
     9661 cgacaaatgc gatgccccat gtatgcagat ttttctctct gataagcaaa atgcccatca
     9721 atatcgtaca cacaatcgta atcggagagg tgagccctgc caggataatg tgaatggttc
     9781 ccgctgtggt tgcttcagca ccacgcacat cctgtggaaa aatgatgaaa gcaattccaa
     9841 gcactgctga caccatgacc agtatgaaaa ccaatcgtgc tattttttgc tgccgcaagt
     9901 ggacaaattg gaaaccaccg gctgcgaaca ggaataagga cagattataa agaatgaaca
     9961 ggctgtttat gatccagcga tcaggctgcc ccgggatgga aagttcactg atggtattgt
    10021 atatatggtt gtacccggga gtgattgccc ctcctacaag cactgccaga acataaaaga
    10081 tcacggcagc caatcctagt tgtggaaaaa atttgttaaa caaagttcct ccgaccaatc
    10141 cttaaagtaa tggcgtccct ggggagactc gaactcccgc ctcaggctcc ggaggccagc
    10201 gctctatcca ctgagctaca gggacatgtg gtgcgaaaag attttaccac gctcgacaac
    10261 ggatagtgta gtatgaatcg ggtaaaataa gggagtttaa tcgaaacatc acaggagaat
    10321 acccataatg cttttgttct atatccgtca cgcgcaatcc gtcaacaacg cactttacga
    10381 taccactggt tcttatgaaa aacgcaatga agatgcacca ctctcagaga caggaaaaaa
    10441 acaactgcct tatctggctg cattcatgca aaaagagatc gataaagtgc aagcggatgg
    10501 gcaagggggg ctgcgaaatg tgcccgtcac gctatattgc agtctcatgg agcgagctgt
    10561 ggctacagct tcagcagtgg ctacaggctg ccagctctca ttgtacgggt acaaagacct
    10621 gcacgaggtt gggggagtat atctgatcga ccatgcaagc ggcgaacata ttggcatgcc
    10681 cggaaaaggc aaagcgttcc tggaagaaaa gtatccgctc ctgcgttttc ccgatgatac
    10741 tcgtgaggat ggttggtgga accgggcatt tgaggaagag aaagaacggt atgggcgtgc
    10801 ggggcgggtt ctggaagcct tatttacaaa ccataaccaa ccagacgagg tggtgatctt
    10861 tgtcagtcat gccgaattct tcaattattt gatcaagcgc atcctctcca ttcccgatgc
    10921 gtttccggta tggtttgaac tgatgaactg cgcggttacg ctatttgatt ttcgagagga
    10981 tcatatccgc attcctttcc tgaacaggca tcatttcatc cccaatgaac tgatcacagg
    11041 gtaaggttat ttcagtggat cgctttgacc gaagccatcg tttctgcaag agaggggaaa
    11101 agatataaag atatacggta gtacctggtc agaacaaaaa ggatatcccg ccagtgatcg
    11161 ggatatcctt tttttcgatg aatctttcga tgcgttgctt tccctgaata tgatggattg
    11221 cactttattg tcagggaagc gattgctcga aaagtttcct aatcagccgg ataaccggtg
    11281 atatcacgaa tttcttcgta cagcggtttg agcaatttat acattttgca atataccctt
    11341 tggtaaagct catcgtaaat gcggtgattc tgtgcgtttg gttcgaacgt ctcaccgatg
    11401 cgggtcatgc ttgaaacggc ggtagcgaaa tcaggatgaa gttttagccc tactgccagg
    11461 tcaattgctg cccctagagc ggatgcttca taggtatgcg gtttggcggc aggcagccca
    11521 aagatatcgg cggtcaattg cgtagcgaat ttgctctggc tgccaccgcc ggcgacgcgc
    11581 acctccgtga tgggaatttt gctgcgccgt gagattcgct cggctccttc tcgtagacta
    11641 tatccaagcc cctccagaat agaacgataa acatgcgcgc gggtgtgcac atccccaaaa
    11701 ccgatgatgg ctcctttggc ttcaggtccg ggctgtttca gtccgggcga ccagtagggc
    11761 tgcaaggtta aaccaagcga cccgggtggc acagaggcaa tcaggtcatc aaacaattct
    11821 tcaacctgca caccacggtt ctttgctatg cgttgctcat tatccgcgaa ctcattttta
    11881 aaccagctca ccatccaaaa tccacggtaa atctggatct caaggctata agcgccgggt
    11941 attgcagatg gatagggagg gatgagcgag atcacttcgg tgtatttgga atgggtggtg
    12001 ttgatggtag cagttgtgcc aaaactaaga catccgatat gggggtccag gcaacccgaa
    12061 ccaatcactt cgcaagcttt atccgccgca gcggcgataa ccggcagtcc ttccggtatg
    12121 cctgtctcca gtgatgcctg ccgacttact tcgcccaata gttccgcagg ttttaccaga
    12181 tcgaccaact tttctttctc gattggtaca gcctgccatt tccaatctga ttttgcgcac
    12241 caggtctgtt ttttataatc gaacggtaaa taacccacct ggctgcccac cgaatcgcgg
    12301 tataagccgg tgaatttata ggtcagatat ccggaaagga agagaaattt atgggtttgt
    12361 ttccaaattt caggttggta ggtgcgaatc cagttcactt ccgcttctga ttgcagataa
    12421 cgaactgtat cttccatgcc ggctgcttta aagaggaatc cccacagacc acgcaccggt
    12481 tctaagccgc tggtacgccg ctggtcgagc cataccatgg cagggcgcaa tggctttccg
    12541 ttttggtcga ggttgatcac cgtacagcgt tgggttgtga cggctaatcc ggcgatggat
    12601 tttttatcga tctcgttctc tttccacagt ttttggcagg cttcgcatag ctttttccag
    12661 taatattcgg ggtcctgttc tgcccaacca ttctgtgggg atacgtaggg aatgatctca
    12721 acctttgatt ttgcgatcat attcccttgc agatcgaaca ccagcgcccg cacgctctgc
    12781 gttccgttgt cgatggttag caataattct ccagccatga atgaatcctc cttaaaatca
    12841 ttgaatcaat tagattatag aatcatttgg aaggcagcga gtaacaatct ttccagattc
    12901 tctgataggc ttgcacttct ttttgccaga tgttctctga ccatttcagt tcctgtttca
    12961 caatcggttt tatcgcatcc agcaccccag cccctccctc ggaagcaagt agacccaaac
    13021 gggtacggcg caacagcaga tcagaaaggt ggcaaaccat ttcattgcgt accgcccagc
    13081 gaatttcagc cagggtataa ggggtgctcc ccagtggctg aaactcatcg gccttcgatt
    13141 cggaaagaaa agtagggagc aggttgccat aaatgccaga aaaacgtttt tgtggagcgt
    13201 tgtcttgcag tgattttgct gaaccctctt cggtcgggct tactgattga tgattctctt
    13261 tttggggtgg aagagatgga cggatttgcc tgatcaggtc ctccgccatg cgttggaagg
    13321 tggtgagttt gccgcctgca atcgaaaata cgttttcgtc tttgaaaatg cgatgggtgc
    13381 gtgatgcttt ggaaggatcc atgcctccca tggaaataat ggggcgcaat cctgcaaatg
    13441 aagaaacgat atcccgttca tccaggtgga gattgggaaa aagcctattg gctgcctcca
    13501 acaggtaatc catttcctct ccagtcatga acggttctgg tttctcccgt tcatattcct
    13561 cgggatggtc aagatcggtt gtcccgacca tggtcgtgcc ctgccagggg ataacgaaaa
    13621 gggctctgcc gtcggatgga tgaaagatgg taaaagcata ttttaccggt gcgcgctcct
    13681 gtttgaagat aatgtgactg ccgcgcagtt ttctgatcgc tttttgtgtg tgtagtttca
    13741 cccgcaggtc atcggtccaa gggccggttg cgttcacgat cgcatgagcg tgcaattcgc
    13801 tttcctttcc agaaagcata tcccttatct ggacgccttc aattttacgg gacgcatcct
    13861 gtagaaaccc ggtgcattgc atataattga aagccatccc cccagccatc actccttcat
    13921 tgagcaccct gtaaaccaga cgcgaatcat ccacttcggc gtcataataa tacagcactg
    13981 catttaaacc ggaatgattc aacccgctga aatttgcagc gatctgagcg ctggtgtatg
    14041 agccgtggtt ccactttgac ccaaaaagat cgtagatccc gatacctgct ttgaagaagg
    14101 acgcactgta cttataatgg tcgtagattg gcagcaggaa ctgcagaggg cgcaccaaat
    14161 tgggatactg tttttgcaga ttttcccgtt cacgaaccga ttcatatgtt acctggaact
    14221 gggcattagc caggtaccgt aaacccccgt ggaccatctt ggaagatcga ctggatgtgc
    14281 caaagctgaa gtcattttga tcgatcaaaa gaacccgata tccctcatta gccagattac
    14341 ggaaaattcc tgcaccggtg atcccgccgc cgatgatgag tgcatcccag atttggtttg
    14401 ctatgtccga acgttcaagg atctgattgc tgttgatcat actttttcct tcaatataat
    14461 ttcccagggt tcatgatgcc atcaggatcg aatgtgcgat aaaaattttg tatcgagcgc
    14521 ataccgaggg cacctttttc caacggcagg taggggcggt gatcgatccc tacgccatgc
    14581 tggtggctga tggtgccatg attctttacg atgacatcac tggcggcttg tttcagtttc
    14641 ttccaccgct caagggtttg gtcagcgtta tcggtgcgcc ggaataaata cgtcacatat
    14701 aaactggcac cgtcagtgta catgtgcgag agatgactga atacgatcaa ccgttcgttc
    14761 tcatcttgca agccattaaa aagcgctgct tcgacttcat ctttaacagc cgctaccctg
    14821 gaccatggaa aaacagattc aagtgtgtca agggcaaaac cgcactccca caggttgttg
    14881 cgcagataag gcgtggtaaa gcgagaatcg aaccacaatt tgccgatgaa tctcttttct
    14941 gtgaagccat ggtgtttgcg agcgatgtgt tgtacctggt gcaaggtggt gcgcgcaacg
    15001 acgggtgagc cggtgattcc gaacagcatc atacagcgtt cttcacccat gccgatcagg
    15061 cgcaatcctt gatctgccag cttgaccagt ttttcttttc cggataactg cagggtggtt
    15121 tccgtctcga atttgtcaag caaccgtagc atcgatacgt ccaggcgggc ttgaacgatc
    15181 tcgcgcagag ccgcgaaacc gctgtcccaa tcgggataaa aaaccgcgta aaaatgctcg
    15241 aactgtggac ggggagaaat gcgcaccgtg gcttcggaaa tgatgcccat gcgcccttca
    15301 gaaccgagcg cacaatgacg cagatcgggt ccggcagcgg aagcggaaaa tggcggcatc
    15361 tccagcaaac cttgcggagt atgcatcaac cctcccagaa aaaggtgctc gattcgaccg
    15421 taataatagc attgcgttcc caccgaacgc gatgcgatcc aaccccccag cgttgaaaat
    15481 tcgtgcgatt gcggatagtg accaaagcgg aaaccatgct gcctgagcgt ttcttccata
    15541 tccggtccgc aaacacccgc tccaaacgta gctgtgtggc tgatctcatc caggtggaaa
    15601 agttggttgt agcgagcaag gtctacactc aatgtcggag tatcgcggtt ttcaacgttg
    15661 atatgcccca ccaccgaagt gccgcctcca taagggatga ggtgaaaatt tccctttttt
    15721 gccaaatcga tcagcgtgtc aaaatcgtct ttacgagatg gcatggcaac cgcatcggga
    15781 aaagtgtcga cagtgccata tcgtaaggcg acccaatcag gaaagctctg accgcgcgca
    15841 tggcgtaagc ggatctcagc atcggttgtg taaagcttgt tctctggcag gcgtgagttt
    15901 ggcactttct tcaaaacagt ttccagggtg gcatcgggta tgatttcccc tttccctacc
    15961 atcctttcga cgaactgctt tgagctttcg gcaaaatgat aggtatagtc cgtttttccc
    16021 cagccattcc atcgtttcat aactcctcca aagttgctat tgaatttctc cagaattgga
    16081 tgctgtcttg catcattttc tgggtacagc gaaaaacaga atcaaacgcc tgttcctgcg
    16141 tgtgctgaag aacattgcgg tagaaaaatt ctgaggctgc ccttgcatca gcttggtcga
    16201 aatatctttc cgccatcttc agataaacct gctcaaaacc gttgagaatt aaaagaaaag
    16261 caggatttcc tgaaagaaat gacgcttttt tgtgcaattc ccaatctaac agaccagctt
    16321 gcgcgggttc attggaaatg gggatgattc tgatgagaaa gtcggtgaga tctgttgggt
    16381 gaagacgact ggcgttggtg aaataggttg gcgcaagaag caagcgcgtt tccaaaaggc
    16441 tctcgacgat gttcagagag atctgcgcgt tggcatttgc cagagcgttg aggacattca
    16501 aattgccctc ttgcagatag ttgcgcaccc gggttggttt gccctgatgg atttcgaccc
    16561 atccatcacg acgcatacgc tgcaacgttt cgcgcagcgt ggggcgggtc actcctaatt
    16621 gcgcacagag ctcgcgttcc gagggcaaat aggaatcaac ggggtacaca ccttgaagga
    16681 tcgcttcgat cagacgtttt tcggtaaaat cggtgggttt ctcagggggt tcccagttca
    16741 tattctccca actggtaaat ggtcttacca gttaaaggat ataaaactta tgcacgcatg
    16801 aaatgtaatg aaagtaatgg tagagaggtt atgtttatct taaatcgagc agaccttgca
    16861 ctgccttcaa cagttcatca ggctgtgtgt gactgaagag tcgtatcttc tgctgccgat
    16921 ctaacgagag gggtttcaaa tactggttga caaatttgcg aaagaagatc agggaaatgc
    16981 ccacaggata atcctcaagc aggagcccca aatggctttg catgagatcg aagacttctt
    17041 tattggaaac ttcttcacga tctctataag aaaataacca gggattcgca agggctgcgc
    17101 gcccaaccat gacggcgtca caaccggtga gatcacgcat ttcgtgtatc tctttcacgc
    17161 gggtcacgtt tccatttccg atcactggga ttttcaggat atctttgatc tgtcggatgg
    17221 ctgcccaatt ggcttttcct tcaaatcctt gttccttggt gcgggcgtga accgcgatca
    17281 tggcgcctcc atgctcctga atgagctgcg agatctgcag gtagttttgc gtgcggtcat
    17341 tccagccgag gcggatcttg gcggttatgg gaatggaaaa agcatccgtc atgcggtcaa
    17401 aaatctccgc gatcttggca gggtcaagca gcaagcccgc tcccgcgccg cggttagcca
    17461 cgcgagcaac cgaacagccg atgttgatat cgaaaaagtc tggatggaac tgcatcagtt
    17521 ttttgcccgc ctgcaagata ttcaatggat cactatcata aatctgaaat ccgatagggt
    17581 gttcttccgc ccgtacgcgg gtaatttttc gtgtgtagaa aatggaatca aagagcacat
    17641 cccgggcgtt gataaattct gtataacata aggtggcgcc ttgcctgcac aggatggtgc
    17701 ggaaagcgga gtcggttata ccgtccatcg gggcgacaac caatccggga ctaaccggga
    17761 tatcgccgat atgaaaacgc ctatcgttgc cagcaggtga gtcgttcatt tccttgataa
    17821 cgaataaatc accgaaaagt gcggttaact tccggattcc tttacttttt ccgcaatagc
    17881 agccagatgg gcggggctgg ttccgcaaca gccgccaata atcacagcac cttctgccac
    17941 ccattccttc acctggcttc ccatatctgc aggggtggta ctgtagttta tctttcccgt
    18001 ttcgtcgacg gttggcaagc cggcattggg tttaaaccag atcggtttgt ctgtaacctg
    18061 cttcagttct ttgaggcatc tcaagttatc ttccaggctt ttcccgcaat tgatcccaaa
    18121 cgctgccagg ttgaaactgg agaatgtctg taccatctgg gtgggcgaga ctcccatcat
    18181 ggtttttact ccacgatcat agctgaaaga gcagatgatg ggcaggtcac acatcgaccg
    18241 ggcaccctga atggcagccg acgcctcttg gatatcaaat tgtgtctcta tgaccagcag
    18301 atcgacccct gcctcacata acgcctgcgc ttgctcagcg tatatattgc gggcatcgtc
    18361 caccgccagc aatcccagcg gtttcaacat ttgtcccagc ggtcctatgg aacccgccac
    18421 caataccgct ctgtctgcca ctgcgttctg agcaattttt acagccagtg tgttggcttc
    18481 tttcacctta ccactcaaac cggcgtgctc caaacgcaat gcatttgccc caaacgtgca
    18541 ggtgagcaag atatccgcac cagcccggat gaaatcaaga tgaagttgct ggatggcatc
    18601 tggattttca taaagccaaa tttccgctgg ttttccttta tccagaccat gctgctgaag
    18661 gctggagccc gtggcgccat cagaaacgag aagttttttc gatttcaaga attcattgaa
    18721 tttgttcatt gcggtacctg tttctagaat agatagaatt tgatcagact aatggctatt
    18781 ccagccagaa cgagataaat tgtttcaacc ttctctttcc ataagagcaa gagacctatc
    18841 agagcaatag ccagggtaaa gacatcgtta atggctgatc gaaaaatgcg atatgctacc
    18901 accaggatca aggcgatcac tccaattgtc acaccctgga gaaaatcgtg aaatatgccg
    18961 ccttttttga tgttcggtat ccatttcaag atcagcagaa cgatcagaaa agagggcaaa
    19021 aagatgccta ctgtagcaac cactgcgcca gggaaacttg cgatctgata cccgatgaat
    19081 gtggcggtgg acgaaaccgg accgggtgtg acctgaccga ccgtgatcgc gtcgattaac
    19141 tgctgctggg ttagccaatg gaatgtattg acgacatcat tctggatgag cgccagcagc
    19201 gccagcccgc tgccaaacag gacggctcct gtctttagga agtatagaaa aataaagggc
    19261 acagaagttc gcggcaggtt gagaaaacct tcagatatta gaggcaggag aacgagaagg
    19321 ttcgcttttt cctttggttt atgtggcagg tgtagtaaaa cccagaccac gccagccagg
    19381 atgatcacca gcgtttcgtt cagaccaagc agcgcgacta ccatgaaaac gacaaatatc
    19441 gtcgcggctt tccaatcttt taaaacctct ttgcccatgt ggaaagcgga tgtgatgatg
    19501 acgctgatca agagcggatt aatagcgtaa aataccgcct caacctgtgg aacattgccg
    19561 aatttggaat acagaattcc taaaatcaga ctgaatagaa aagaaggcaa gataaaggca
    19621 ccaccggcga tccataatcc ggcattgccg gcatagacat agcccagatg caaagccatc
    19681 tcaaccgcat tgggccccgg gatgaggttg gtgatcgcca gcatgttcag aaaatcttcc
    19741 tggttaaccc atttgcgctt gtgaacggtc tcctgctcca tcagtgcaat gttggcaagg
    19801 ggaccaccga atccgatgat ccccaatttc ataaaaagga gagcgacttc gtatagctta
    19861 ttgtttgcct tcatgccgtt tttctggatg caatgccttc catacttttt cgggggtgag
    19921 tggcaagtca tgcagccaga cgccgctggc attgtgaagg gcattgacca gtgcaggggc
    19981 tacaccgtcg atggagatct ccgcaatcga tttggcgcca aatggaccgc ttggttcgtc
    20041 ggtttgaaca aaaatcacgt cggttggggg catctcgttc gccagatagg tatgataggt
    20101 ctcaaaacga gggttaagga tttgcccttt ttcatcgatg taggtttctt cacaatgtgc
    20161 aaaaccaagc gcttgcgcca tgccgccttc tacctgaccc gcagcggtga tggggttgat
    20221 cacgcgcccg caatccacga ccatcaataa ccgttcgacc ttgatctcgc cggtttctgt
    20281 gtccaccgcg acttctgcga actgtgcggc ggtgggcgga gggctgacat aactgcggtg
    20341 cgattcggtt gccatgattt ggtgttggtt tagctggtgc aggctgctca atgccacttc
    20401 ttgcaaagta acagacttcc cttcagggga gaccactcgg gaatctttta gggtcaactt
    20461 ttcgggatca tcccatttga gcatcaatgc cgcctgttcc tggatttgct ttttgatggc
    20521 tgcggcagtt ttcttgaccg cgcccccgct gatataagtt gtagaactgg catacgcgcc
    20581 tttatcgaat ggggtgaaat cggtatctga tgaatagacg atgatatcat ccaccgtgac
    20641 ccccaactct tctgccgcga tctgtgccag aatggtgtct gatccggtac cgatatcggt
    20701 ggcgcctatc agcagattga acgatccatc atcgttcatc ttcatggtag ctgcagccat
    20761 atccaacccg gcaatgccgc taccatgcag ggcaatggac atgccgatgc ccttgcggat
    20821 cgtacctgat tggttcttgt actcttttcg tttttcgtag tagcgggtgg ctttcaagcc
    20881 gatatccaca cactggctca acccagaaga agccattgac tggtcgaacc cttctcgtcc
    20941 ctcgccaagt ttctttgcca tatgcatcgg ttcaccgagc ttcagccagt ttttgcgttt
    21001 gaattcaacc acatccacgc ccaatttttc agcgatctct tccataagca cttccaatgc
    21061 aaagagttct tgcatggtgc cataccctcg ataggcgcca gcggtgggag tgttggtgta
    21121 caaaacatcg caaatgaagc gcgagtcgaa agcgttatat agcgttaacc ccttaaaccc
    21181 gccaaccatg ctggttgtca aaccgtgcgt gccatacgcg cccgtgtctg ccagcagctt
    21241 caattccaca gccgtgacta tctcatcctg cacagccacc ttgtactgga tatagtgctg
    21301 gtgccgggtt ctgccactgg taaattcctg tcggcgcgaa tattcaaggc gtaccggtct
    21361 gcctgtcttg atggttaaaa gagcacacaa gtcttccagc aggatctcct gctttccccc
    21421 aaaaccgccc ccgatacgtg gtttgattac tcgaatgttc ttgatgggaa tgccgaccag
    21481 cggagaaatg atgcgtcgca catgataggg aacctgggtg ctggaaagga cgaccaatcg
    21541 atcatctgga tcaaaatagg tgatacagat atgcggttcc aactgtgcat gttgctgttt
    21601 gggagtgtga aattcaccgg cgacaacatg atctgcctca gcgaacacct tgtctgggtc
    21661 accattttgc gcttcaatgt gatacacgat gttgtgcttt gcatcgtaaa taccttctgt
    21721 atcctcttca tcatggatga tcggcgcccc gtctttgata gcttcgcgtg gatcgacaac
    21781 tgcctccagc atttcatagt ctacttcaat gagctgcagc gctttgtcag ctatttcctg
    21841 ggtgtctgct gccaccactg ccacgcgatc acccacatgg cgcactttat ggtccagact
    21901 aacctgatca taagggagtg gctgcgggta gctttgtccg ccagaagtgt atttcacgcg
    21961 tgggacatcc tgataggtca gcacagctcg cacaccagca agtgccttcg ctttttcgat
    22021 gttgatcgaa cgaatgtgag catgggctac attgctggtc aatatggcgc caaaaagcat
    22081 cccctcgggg cgaaaatcat cggtgaaggc aggtttaccg gtaacaagtt tttgtgcgtc
    22141 aactttttta ggagtctggt tgaccacgtc tgttttggac atggatggag gggtgtaaac
    22201 caggggaggg agcggatgct cgccgccgtc ctttcgcaga tacggttcgg gtatgtggat
    22261 atctgcaaat ttttcgagca gatccagata caatggtccg taatcgtcca gttttttacc
    22321 ctgcattctt gctgcagctc gcatgacgcc ttccacaatg cgcacatatc ctgtgcagcg
    22381 gcatagaacg ccttttagac tggaacgcac ttcttcttct gttgggttgg gatttttgtc
    22441 aaggaaagcc ttggcggtca agatctgtgc tggtgtgcag aaaccgcatt gaatggcacc
    22501 tgtttccatg aaggattctt gaagcgggtg cattttcccg tcctggttca atccttccag
    22561 agttaaaacg gatttcccct ccagatcctt tacttttagc atgcaagacc gaaccggctg
    22621 cccctccaaa agaatcgtac atacgccaca atcaccaacc ctgcacccag atttgatgct
    22681 tttgagattc aaaccacgga gaaaatccag ggcgttctgg ttagaatcag cactgcccgt
    22741 ccatgtggat tgattcagtt caaatttcac agcagtctca ttacccatta caaatctccg
    22801 ttgatctttt ttgacaaaat tcgttcaacc aacactcctg ccatgtctct gcggtaaact
    22861 gcgctgccca tataggtatc aacaggagtg atcatttctt taaatttttc actgatggct
    22921 ttcatcagaa cactatcaag acgcttgccc gacatatctt cggagaagtc ggtgcaatca
    22981 tacgcagctt ttgaaactcc ggtggctgca atgcgcaact gatcgatcat tctttccttg
    23041 aacttgaggt agatgatcac agagatgatc ggtcgatcgg ctggagtatt gctaactttt
    23101 tcgtaagcca gcagatcaga ttcatccaaa tcaagttgaa cctctttcag ttcattcagt
    23161 gagcctgccc ctttttcaag caaggagata aaaggcgttt tctgaccagc tgctaaagta
    23221 gctgtgggat tgatggcgtt caagcctgct ccccaatcag gaaagctatt cctggcgcgc
    23281 acagatacaa ggaattccgt ctcggctctc tcctgaaaag tattttgttc caggattcta
    23341 tgtagggtgg ggggcaaatc ttttcgtgag taaattgctt ccaatgtttc cgcagaggtg
    23401 atcgtcagtt tcatcatctt ctccaagatt tatactttga ttaattataa ggttgaatga
    23461 ttgtgaaatt gtacctgctc attatacaaa attgtaccgt tgaaaaggat gaagatatcc
    23521 agcatcttac acatccgttg gcacaacttg gcgagcagca acgagaactc ctgcatctgc
    23581 gcttttcggc aaattatgga attctcccat tgggattctg agaaggtatc acagaaaaaa
    23641 gggataggaa gcgaaattcg ttatcaggta aatcctacct gtatggtagt attatattgc
    23701 cactggcata gtgagcaaaa gacacatggt aaaaaaatgg aaaagataat tctggctttt
    23761 ataaccggat tgaccacggg cggtttgagt tgcttggcag ttcagggagg gttgctggct
    23821 ggctcactgg tcaatcaggt ggaaactgat ctggcacata cgaagaataa gaggaaaggc
    23881 acacagcaac acgacaaggc tttccatctg gcatatccac ttctgatctt tttactgacg
    23941 aagttgatct cctataccct ggcagggttt cttctgggtc tggttgggca gatgtttcaa
    24001 ttgtccactt ttacccgtgc cattctgcag atcgctgttg gcatctttat gctagggaat
    24061 gctttacgca tactgaacgt tcatccgatt tttcgtttct tttcctttga acctcctgca
    24121 ttttttcgac ggttcgtgcg gaaaagtgca actaggcagc aaacatcgtc ggtcttgatt
    24181 ccggcattcc tcggattttt gacggtattc atgccttgcg gagtaacgca agcaatgatg
    24241 gcggctgcga tcgctacggc tgatccaatt caagctgcgg ttttgatggc agcatttacc
    24301 ctgggaacca gcccagtctt ttttgccgtg atgtattttt cgtttcaact tggcgccaaa
    24361 ttggaaagat acttcatgaa gtttgtggcg attgtattat tggtctttgc actgatcacg
    24421 attgatggtg gaattacttt ggcaggatcc ccgctttcca taacccgcat ggtcaataat
    24481 ttgaccaata aaaatgaggc tgacacatct ctgtctcctg aattctcgag cgttcctgcg
    24541 ggtgctgctc ttgatgaaac ttcggaagat tccgcggttt tggaaaatgg aatcaccatc
    24601 acggtaaaaa acaacgggta ttatcccaac gcagtgcatg tgaaagccgg catgccgatc
    24661 cagttgacac ttgtgagtga gaacgtcaat tcgtgtgcac gggcttttgt cattccgaca
    24721 ctcagaatac agaagctgct cgatgtgacc ggggaagtcg ttctcgaaat ccctgctcaa
    24781 gaagagggga cccgcatacc tttttcctgt tcgatgggga tgtatacggg gtcgatcatc
    24841 gttgatgaat aaggggatga gctcatgcag aagatcattt tgaagataga aggaatgcat
    24901 tgtccgaatt gtgcgctggc gctggaaagg gtagaagata cccttccagg aatagaaaaa
    24961 gtggatgcaa gttatcataa agcacggatg gtcgttgagt tcgatgaaca tatcgtttct
    25021 gaggaagcga tcagagacga aatattaaaa ctggggtatg gcatagtctc ctgaagaatt
    25081 cgctgaagcc tcaccctggt cagataagaa accagaattt catatgagag cgttcagaaa
    25141 tgctgttgtg ctttaacgag cgcgccaaaa aattcgctat ttagtccatc tggatgtcaa
    25201 cggatggtac ccccaggaat tggctgaggt tgtgcgcttc ttcttgtatt tcgctcatga
    25261 tgcgtggcga aatggaccca aacttcgtta cttttacgcg caggtgattc ccttcacgaa
    25321 cttgctccca tacagccaca acgcgaccgc ccaccaatac caccggagct atccatcctt
    25381 gcgcacggta aatatgcggt tggtgctctc tggctacaag atgatcccgt tctttatgac
    25441 cgagaaggaa cgtatcaaaa tacggcagga ggcgcaccag tggttgttcg aatccagctt
    25501 gcgccaatac atccagatct tctctaagaa tctccgctgc ttgcccttca acggtgactg
    25561 gcatgaagcc gtactgctca tgcgcccaga tttcgcgggc ttctgtgagc gtcataccgg
    25621 tccacagggc aaagtctgtg gcggtggccg gaccgaaagc gcgcaggtac ctgcgcaata
    25681 gactgacttc ggcttgttct ttcgctacat cttgccactt ggggatccag gcgtcagctc
    25741 ggacgaaggt cggttcgttc ccgcggttgg gacctgagca gacaacgcca cgggcggctg
    25801 cgaggtgcag gaggtctacg actggaaaag ttatttcacc aaccggaaca gcagcgatct
    25861 tcctccggct tccccagccc ccaccgcgga tggtttgcgc ttgcacgccc aggatgcggc
    25921 taacgtgttc ggcgatctcc ggtcgggtgc gcggttgatc caacgcaccg agcgccgcac
    25981 cgagcgccgc atcgattgct gcatcgatga ttcggtcggg cacacccttg ccacgagccc
    26041 agcggatctc tttttcggca cgccgtgcag agccccggac gaagattgcc aactgctcgg
    26101 agggcactat gaactgcgtc tggcgcatgc aagctgcttt gaccagaact cgctcgctca
    26161 gggctctttc aatatttgcc gtgtgtagat cgcgcactct tgaccaaagc gagatctgcg
    26221 ctgctgagag caattgagcc tgaacccctg ccatatcgcc cgcgaccgct gcgagagcgt
    26281 ttgccggtgc gcgctccagg agatggtgcc gagagagacg gaaggcaacg acctggttcc
    26341 aggttgctgg aaattccgca gtttggtttg ttggcacttt aattcctaat tgttctgaaa
    26401 atggaattat atatggttct ggtcattatg aaaaaggtct acggtcagcc gatggaaaga
    26461 ttcaacagcc ctgggcgagt ggacttgcac cttaccgtgt gaacgaaaaa agctacttca
    26521 acggttgtcg aagcagcttt tcaactccct ctgctgttct tccccttaac atgcagaaaa
    26581 aacaagttca atcttttagg tattgttgat aggggctatc agaactttat ctacccgtct
    26641 cccgtccata tcaaccactt cgaaacgaag atttccccat gtaaaatagt cgccacttac
    26701 aggcacttta ccgagctgag acatcattaa accactcaat gtatcgtaat ttccttcttc
    26761 ttcattgggt aatttatcta tgctgatact ttcttttaat tcatccatta aaaccatgcc
    26821 atcaactaac cacgacccat cttcacgctg gattatttca gcctcgaatt ttccataagg
    26881 attcatcatg tctccaacga ttgcttcaat aatatcgttg atactaacca atcctaaaac
    26941 gccaccatat tcatcaacga taattgctat ttctgtcaat gatgctttga gtaaatccaa
    27001 tacatcaaga gcaggagttg actcggggat aaaaatgggt ttgatcagta ttttattaag
    27061 atcaaagtcg ggaccattta ttctttgcaa taagagatcg cgtgattgta tgattccaac
    27121 aatgttatct ggcgtatcct taaacacagg aaaacgtgag aaagggtggg ttgttattct
    27181 ttgataaatt tct
//
LOCUS       DAOSDS010000021        27153 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_820_length_27153_cov_5.240940, whole genome shotgun sequence.
ACCESSION   DAOSDS010000021 DAOSDS010000000
VERSION     DAOSDS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 27153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27153
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_820_length_27153_cov_5.240940"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLL88_04150"
     CDS             <1..287
                     /locus_tag="PLL88_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00786.1"
                     /translation="KASSYVMEYLSESQEVMGCALNDPLALAALFQDDILELHHLVID
                     VDTNYGVSMGKTFADFYNYDHKPANGYAALGVDSRRFVTHFLERMIKFVQ"
     gene            590..1480
                     /locus_tag="PLL88_04155"
     CDS             590..1480
                     /locus_tag="PLL88_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ00787.1"
                     /translation="MKKVNFFVALMIIAALLLASCSTAASPEGVEESAGEEVKSGMEG
                     QVYECLGTAEDALVDLECREITIAVENAYLPFNYISSETGEAGGWDYEVWPAICELLH
                     CKPVFVETAWEVMIQSVAEGLMNTAGDGVTISEERLKQVDFSIGYINVEQRLLVKKGE
                     TRFASIEDFVANPDLILGTQAQTTNYETAVQYLPEDRIQAFEQFPFAVQALIAGDVDA
                     VLIDETAGLGYTGTSAEEIELIGPSISSDELGFPFTKGSDLVEPVNLAIQALKDSGKL
                     EEINMKYFGPTFTLTYDDVQ"
     gene            1562..2440
                     /locus_tag="PLL88_04160"
     CDS             1562..2440
                     /locus_tag="PLL88_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOJ00788.1"
                     /translation="MAVNDRLVTQKNKNGLQTTNEMQLTKKDFPWWMVAIGLLIVITF
                     LLIRIKPDFKDAFNIIRAGMSVTISTTLIAFTIATMLGLFIGLGRVSESIFWRNASTI
                     YVEIIRGIPLLVLIFFIALVLIPGLTNLLSALGTVLIDVGMGSFGAKLAAIENKSISM
                     NVRAIISLAITYAAFSAEVFRAGIQSIDKGQMEAARSQGMSRWQAMRYVILPQAIRNI
                     LPALGNDLISMLKDSSLVSILAVRDITQVARLYAGHSFKFAQAYSILCVLYLTLTLVL
                     SLCMKVIERKLGNERK"
     gene            2448..3176
                     /locus_tag="PLL88_04165"
     CDS             2448..3176
                     /locus_tag="PLL88_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HOJ00789.1"
                     /translation="MIETENLSKSFGTLEAVHGVSLKVYPKQVLVIIGPSGSGKSTLL
                     RTMNWLEQPSEGEVYVDGKSLSENQHHINDIRSNIGMVFQNFNLYGHLTVTENITLAL
                     RIVKKMKKEEAKKIAMRQLMKVGIADKADRYPSELSGGQQQRVAIARALAMEPKVMLF
                     DEPTSALDPEMIKEVLDVMQLLVKEGMTMVIVTHEMGFAKAAADEVIFMDMGKIVERA
                     APEEFFEHPSCERTKQFLSQILKH"
     gene            3274..5034
                     /locus_tag="PLL88_04170"
     CDS             3274..5034
                     /locus_tag="PLL88_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00790.1"
                     /translation="MQNGSYHFYEEKIVLRVRNRVCNNSEELIQSELFAKVLQCFLED
                     LAQRQSPLLTVFPDSHISHDSVKKLTEVLFYLIHLPAAKVEKIVEGSAAFLADPFLLN
                     EFVEQLYNYWRSLHRLIICDSIFDRFDQKPYRTFNDLVESLMHIVRSTYRTIQENITG
                     NHPKIYRQVSAGAEVGAIALPYHINYPDGLYDVLQDVFIIRQALIYPPMIFKTPMNKR
                     NGQFERVDQNPLEKIHLEHAEWLCYPAKVGDLLIMVYFSLDLFELGFSLCNLFELAEE
                     DDLQRKPDAVFLYGVPPECALQVHGNETIFYEDTENDLLIGTVPNKDEFGYFGYLKKM
                     ILTLHNIKRMRTGSLPFHGAMIRITLKDSQPFALVILGDSGAGKSETIEALRRIRTKD
                     IEEIVIVADDMGSFSIMPDGKIAGYGTEMGAFVRLDDLQAGYAFGQIDRTIIMNPDQV
                     NARVVIPVTRYEYLIKGIPVDAILYANNYESVNVDHPSIEEFKTPRDALRVFRRGAVM
                     SKGTTTTTGIVENYFANIFGPVQYQELHDKIASDYFGAFYAAGIYVGQIRTMLGICGQ
                     EQHGPENAAIALLDLIRSRA"
     gene            5115..5906
                     /locus_tag="PLL88_04175"
     CDS             5115..5906
                     /locus_tag="PLL88_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HOJ00791.1"
                     /translation="MNLATFRFYAELNDFLPVKWKKTNFTLPFSRGQTVKHMVESCGV
                     PHTEVDLVLVNGNPVPSSHQLQNNDRVSVYPVFECIDISNISMLCPKPLRHPRFVVDC
                     HLGRLAVFLRLLGFDVLYRNDFPDDELAEISEKEKRICLTRDRGLLKRNQIEHGYLVR
                     ALDPRAQLIEVLKRFQLNLQLKPFTRCTRCNGFLRPVEEEEISSRLLPGTREHFHEFS
                     CCESCGWIYWRGAQYERLQKLVDFVLKELKMERNGLGALKVENHD"
     gene            6047..8902
                     /locus_tag="PLL88_04180"
     CDS             6047..8902
                     /locus_tag="PLL88_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOJ00792.1"
                     /translation="MNALQIDRKKLATLQAKDIYPLLESLPDGLASGEVLARQKRFGL
                     NVLQSEKKTPLVIKLLANFTHLMAVLLWVGGIAALVARMPQLAVAVWMVNIINGAFSF
                     WQEFRAEKASEELQKLLPTYTRVLREGKEEKILAEELVPGDVVLFAEGEKISADCRLV
                     EQAAFRVDQSTLTGESRPVGKSADASTMEEITLTEQPNLVFAGTSVAAGTAKGIVYAT
                     AMETEFGKIAHLTQSMKEELSPLQIEMKSVTKTITIIAIMVGIVFFILALFLAKIDLA
                     ESFVFGIGMIVAFVPEGLLPTVTLALAMGTQRMAKRQALVKKLSAVETLGCTTVICTD
                     KTGTLTQNEMTVRRMWIPQKESKREFHADGRIEVSGIGYNPSGSFHWKDKPASSVQDE
                     DVQEILRAILLCNNARLIAPQGEETAWKILGDPTEAALKVAALKNKMNEEEELQAHPR
                     IVEIPFDSRRKRMSTLHLEQAHGLQGATFVQAPRVAFVKGAPKELLDLCAHISISGEV
                     FPLSEEQKQAAILENDTMAREGLRVLAVAFRRLPKDFGSQSAEDIERDLTFLGLVAMM
                     DPPREEISAAVQKCNQAGIRVIMITGDYGLTAESIARRVGIVRSANPRIITGFELDKL
                     SDEELKDAFQNEVIFARVSPEHKLRVVMALQNLGHIVAVTGDGVNDAPALKRANIGVA
                     MGITGSDVAKEAAAMILLDDNFASIVNAVEEGRAVYANIKKFTTYIFTSNTPEAVPFI
                     LYAFTAARIPLALNVMHILAVDLGTDIVPALALGAEPPEPGIMQQPPRDLNTHIITPK
                     LLKRAYLLLGPMQSLASMAAFYYYYWTNGFAGQWMDLPATGALYQSATAMALAAVVTT
                     QIGNLFAQRAESTPIYQMPLFKNKMIWVGILTELVVIFAIVYVPFFHQFIGTNSFSLK
                     YWLFLFAWIPSLIMVDEIRKWVERKRTLRGLSKGGVR"
     gene            8899..9543
                     /locus_tag="PLL88_04185"
     CDS             8899..9543
                     /locus_tag="PLL88_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOJ00793.1"
                     /translation="MKVIVIGCGRLGINIAREMDQRGNEVVVIDKDALTFENLGKTFR
                     GSTVVGIGFDRDILAKAGVDLADALVAVTSSDEANVVTARIAKFVFRVPKVVARVYDP
                     RKAEIYRRLGIPTISPVTMAGERISEMLSFSQLKTVMTIGSGEVLITEVEMTAPFQGK
                     KMAQLEIPNVAHPISLTRDGKTMMAMPNQQLEMGDVVHLAVVASMTDRIREMLK"
     gene            9558..10220
                     /locus_tag="PLL88_04190"
     CDS             9558..10220
                     /locus_tag="PLL88_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOJ00794.1"
                     /translation="MKVLIIGGGKVGTYLASHLLDGSHEVTVVEPRREELERLQSELG
                     AEHVILGNGTDPVVLESAGIRTMQVVAAVTGSDETNLVVTNLARFEFNVPRIIARVNN
                     PKNNWMFTADMGVDVALNQADLMVHLILEEMSMGDMMTLLKLRRGKFSIVEEKLAPQS
                     SLTGARLEQAKLPQDSVLLAVMRKDDILIPHGNLELKAGDELIVLVHSAQLDQLEKIL
                     NS"
     gene            10377..11534
                     /locus_tag="PLL88_04195"
     CDS             10377..11534
                     /locus_tag="PLL88_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOJ00795.1"
                     /translation="MHNTSRIGVVFLLIVVSVVSGFLGAYIGGNMVAANLAADAEEQV
                     IPTPTLNTGEVQVVNQTITISSSEIDTVITDVVEQIEPAVVTVVGTIPGQQTFFGRTA
                     DSEVSGSGFIISSDGYIVTNNHVVEDASALSVVLSDGMQLKAEIVNRDVYADLAVLKV
                     EGAMPAVAALGSSADLKPGETVIAIGSPLGTFRNSVTVGVVSATGRTLDTGEGYNMEN
                     LIQTDAAINSGNSGGPLVNLAGEVIGVNAVVVRGSSSSSASAEGLGFAIPSDTVKVIS
                     EQIIEKGYFARPYLGVGIQNITASIATRYNLPVAWGGYITEVVAGSPAAGTGLRAHDI
                     ITRLGDIGITEGTTYLNALFTYQPGEEIEVEFYRGSQKMTVTVTLAETTAQ"
     gene            11557..12381
                     /locus_tag="PLL88_04200"
     CDS             11557..12381
                     /locus_tag="PLL88_04200"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOJ00796.1"
                     /translation="MTIHSIEPIRLICSDVDHTLLTEEQQLLPMTEATLRRLQERGVK
                     IILATGKNLGATRAIADRLELDDPLIFVNGSLIQYRDGKIFSQHELQPEITEQLIQLG
                     ESQGMDMMLYVTNDIFVKEGGKYSHALDKYGGPKPIELKDWQDMGANLAHILKFVFID
                     EDFSRLEGMKVILEKNISNGIELCFSLPILLEVQPAGVSKGSALEKVAKLLELPREAL
                     MAFGDGNNDIEMLQYAGIGVALENATPELKNVASHLAPSNNEEGLARFLQDFFNLQ"
     gene            complement(12368..13300)
                     /locus_tag="PLL88_04205"
     CDS             complement(12368..13300)
                     /locus_tag="PLL88_04205"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ00797.1"
                     /translation="MDELNKRRTKLFLLFAFGISWLTALVIYVTGGLTNSPIVMEESG
                     ISLAYILLASAYMWGPAIANILTRAVTKEEKGTLLLKLDFRANWRYVLAAWVGIPVLV
                     LLGSALFFVLFPSYFDSGLTVLSSQLAAYGADSSASFIYQFIIMQIAYAILVSPILNM
                     ISTFGEEFGWRGYLLPKLVPLGKRKAILLTGLIWGVWHWPVIAMGYNYGFDYPGFPWL
                     GPLAMVWFTTLVGIFLAWVSLKSESVWPAVIGHGALNGIASIGVLFLVKNPPALLGPY
                     PTGVISALPFLLAGGIILLLAFPEDKPEAVITAN"
     gene            complement(13332..14204)
                     /locus_tag="PLL88_04210"
     CDS             complement(13332..14204)
                     /locus_tag="PLL88_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HOJ00798.1"
                     /translation="MSLTTAQKDRILVLQQEELDGHYTYLKLATAVKDEHNAQVLQRI
                     AEEEMKHYQVWKRYTQRETKPKKWRVQFYYWLAKVLGLTFGVKLMELGEEKAQENYKE
                     LVDLIPEARQILAEEEQHENELLEMLDEESLKYAGSVVLGLNDALVELTGALAGLTFA
                     FQNTQTIALAGLITGISAAFSMAASEYLSSKSEEGEKSPVKSAIYTGFAYILTVAILI
                     FPYLVAKNFLVSLAWTIANAILVIAIFNFYISVAKGFDFKKRFFEMSLISLGVALFSF
                     LIGNLVRRVFGIEV"
     gene            complement(14581..15864)
                     /locus_tag="PLL88_04215"
     CDS             complement(14581..15864)
                     /locus_tag="PLL88_04215"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HOJ00799.1"
                     /translation="MTQDPIANPLMGWAPWATIQESEQPYTLVYADLTWREFEPQEGI
                     FDFKSFELKNQFERWRAEGKRVVFRFVLDVPGSEPHMDIPDWLYAKINGDGDFYDSDY
                     GKGFAPNYANPILIEYHQKTIAALGERYAQDDFIAYIELGSIGHWGEWHVKSDSGIRS
                     LPETEIRDIYVNHYLAAFPNTFLLMRRPFSISENNKIGFYNDMTADYSSTITWLGWIA
                     DGGSFDQTGESNALVALPDQWKIAPIGGEQTPSLKDTEVYFTSLSQTIELLRQSHTTF
                     IGPNGVYEGEEDGSLQEGVDQVLSNIGYRLYIEKIKLPLKIHAKGPATIVITFSNGGV
                     APMYYQWPVFLYLFDDQNNLVNFFSIEMDVRSILPGDSSVVKYDLSVEGLKNGRYSLG
                     IAIHDPKTNLPAVQFAMANSRDDSIQILASFEIDR"
     gene            complement(15993..16658)
                     /locus_tag="PLL88_04220"
     CDS             complement(15993..16658)
                     /locus_tag="PLL88_04220"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOJ00800.1"
                     /translation="MFNTIISFIQNDLVDALYSLLPELTSLIFSFLLGIFIFYVYRRF
                     YKGIIYNHGFSITLAAMTILSTIITLAISSNIALSLGMVGALSIVRYRTAIKDPIDIM
                     FLFWGVATGITIGAKMHYLALIGAVIVFILFLVLNSQKSNKKMYILLFHYSMSDTEEE
                     LKQILRKNRYQIKSKTMRQEKVEIAIELVVRNENFAFMEAIKELPTVTDVTLVQYEGE
                     YFN"
     gene            complement(16686..17387)
                     /locus_tag="PLL88_04225"
     CDS             complement(16686..17387)
                     /locus_tag="PLL88_04225"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOJ00801.1"
                     /translation="MAYRHELKYIIDEANGSLLQLRLKEVLKKDLHASPSGFYTVRSL
                     YFDDYFNTAYNEKDMSILDREKFRIRLYNHSDQVIHLERKVKSNNYVFKEIAPLNRSE
                     VEAILDENFVALRNSDSQLKMIFYHMCTSHFLRPRVIVDYEREPFIMEAGDVRITLDR
                     NIRSGMEALNIFNHQMAMIEALEPNYLILEVKYTNFLPTIIKDILPAPHAVYTSMSKY
                     VICCNKTMFKRRTSE"
     gene            complement(17389..19191)
                     /locus_tag="PLL88_04230"
     CDS             complement(17389..19191)
                     /locus_tag="PLL88_04230"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOJ00802.1"
                     /translation="MKKIFRSLLVIIGILAALLISFQSLEANLEEPEVEIDIQDELNI
                     DREQIVIEQPTEINDLPLKDNPDIYLNDDPDSIVYMYVTVRRGNSGDDTDHTWQEVND
                     SSKFFVEGTINTSVNKAEAILQIGDENGPLPGQFGYGEIVPNAIIQVRGNTSSLEAQK
                     SYKIELFDSAGEWRGQSTIALNKHQSDLTRMRNKLGFELMENLDGLISLRTQYVNLFI
                     KDETTSPPSDTFMDYGLFTQVELPNRKFLRNHLLDQDGQLYKANMFEFFRYPDTLRLP
                     DDPLYNRDLFETVLEIKGNQDHSKLIQMLDDVNNLSIPIEQIFETYFDAENYFIWMAF
                     NILIGNLDTNSQNFYLYSPQNSQKWYFIAWDYDGILARQERQLLGRSNPLYFEKGISN
                     YWGCVLHKRILSKAEYREQLDKKINELMAYFAPERIAEAIATYRIVTDQYIAKMPDIL
                     YLPGTIDEYNTVLDILPDEIQINYQLYLDSLESPMPFFLGDPQIINNMIIFSWDEAYD
                     FDAQNITYQLSISKNWNFREIVFQTEVVNFLSVELNLLPPGEYFWRIIAVNEDGKMQY
                     PFDYYTDLNGVEHPGIRNFYINQENQNITITSGR"
     gene            complement(19235..20644)
                     /gene="pelG"
                     /locus_tag="PLL88_04235"
     CDS             complement(19235..20644)
                     /gene="pelG"
                     /locus_tag="PLL88_04235"
                     /inference="COORDINATES: protein motif:HMM:NF028243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HOJ00803.1"
                     /translation="MAGIGFELKKLYASRGGFSKIRAVFYSSIVIAGPMIMGALLLFG
                     QKYLSIQFGASSHEQDVIIVITTYSLLFPLLLTSFFSYMISRYIADKLYQGEYKNILP
                     SMYGLTSILLLIGSLGWGIFLYLADIPFHYAILSFILFCSALVAWVQITYITAVKYYR
                     DILSGFIAGIFTGLLAGVLLILLFQAEVVTAFLTSACIAYGIMVIVYVYALHKYFPLR
                     ALKHKETFEFLKWIKKYPSLSMVGFFTTLGLFIHLMLMWKSPWGIQVIGLFYHAPMHD
                     IPALFAFLSILVTTVNFVTSVEVNFYPKYQVYFGLINNGGALTDIKKAHSEMLIVLKQ
                     ELFYLALRQMFATIIFVVIVSGIISNLGLGFTHEMIGLYRVMCVGYAFYAIGNSMMLI
                     LLYFVDFIGAVFSGFTLLLVNTIGTLYTISLSSNFYGFGFLVAGLTMCLVTLSRLSFY
                     TRRLDYFIFCKQPIFVKNN"
     gene            complement(20620..22047)
                     /gene="pelF"
                     /locus_tag="PLL88_04240"
     CDS             complement(20620..22047)
                     /gene="pelF"
                     /locus_tag="PLL88_04240"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HOJ00804.1"
                     /translation="MKICLFLEGSYPYVQGGVSTWVDGFIRALPRNEFILWTIVDSPS
                     RKGKFKYQIPSNVLHIYENDLDSPLRTRMVHGSFMKLSKDEITALSQLIQCKNPDWDI
                     LFNCFGGNEKKAISLFKSHAFLEMLKEFALDEFPFVGFTDLFWTVRSMFLPILSLISQ
                     EIPEADLYHSLSTGYAGVMGGISARKFNKPYILTEHGIYTREREEEILRADWVVPHFK
                     ELWISMFYMYSKFAYQSAHVVTSLFKRANDIQQEIGCPATKCEVISNGIFCKEYSSLP
                     VKEESNWVDIGAIVRIAPIKDIKTMIYTFSRLKQEIKNVRLSILGSVDDEDYYKECID
                     LIEYLDLKDIKFLGLVDVKSYLKQFDFTLLTSISEGQPFAAIESLAARRPLVATNVGS
                     IRELIDGDGRDSLGAAGIYVPPMHQTELLHALMKMCQNKEMRYKMGEIGQKRVMKFYE
                     HEAMVQNYLSVYKKAEIKWQELGSS"
     gene            complement(22051..23898)
                     /locus_tag="PLL88_04245"
     CDS             complement(22051..23898)
                     /locus_tag="PLL88_04245"
                     /inference="COORDINATES: protein motif:HMM:NF021477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2194 domain-containing protein"
                     /protein_id="HOJ00805.1"
                     /translation="MKNSTVSFSWLLIPILILSTIGFGLLVERSGIPYKAESSPLSFL
                     NPMTTTQPPVESEQKNDCLVLYDSESDVDLKQFTIVKDTLDSMRVGYDFFNVAEIAST
                     FPLALENYETIVITFYALDAISQQIVDITRWVENGGRLLITIRPDISESFNAIYRKLG
                     IVSYDDSGTQPISEIEFISDLFPGMKSQTLDVGSVNNSSLVLQLEKSAHVHLRSLDAS
                     KTPILWDYDFGKGRFVVINSDQFANKYDRGMICAAYSLLYDTFIYPVINSSMVFIDDF
                     PAPIPEGINDLVFQEFGRDIESFYINIWWPDMKDFARRYGLKYTGLLIETYNDKTSPS
                     FIEETSVEQFSYFGSSLLSIGGEIGLHGYNHIPFAISIDETIAELGYPIWTSFDSIHL
                     SLEALKTFAESLFPEQQFITYVPPSNILDPEVRTWLPTVFPDIKVISSSYLPDENSDA
                     YYVQEFEEAPDGIIEVPRIISGYEFASIMRWEAASELVLHYVNSQFIHPDDVLDERRS
                     SGYGWGYLRDNLEQYFIWLEGAAPEIRQMTASEGGKAVQRFSRLELNTNCENTTCTIQ
                     LGNFYDEAWLLMRTSSTPTNIIGGTISEVTPSLYLIGAESAQVKIAFEE"
     gene            complement(23895..24719)
                     /locus_tag="PLL88_04250"
     CDS             complement(23895..24719)
                     /locus_tag="PLL88_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00806.1"
                     /translation="MGRSNHQKEFIPILFFIPFFGVLAALIIDMYYKAEGHNQNPIEI
                     YQLSPGENIYWRPIENRKENINLIPLEEAISLNDTKTRRKLMLESLYDNPSKFIDVLL
                     VARTNDDIETVHYASTTISKIQRDFQIELQKLSVAIEKNPEDIQLLDTYIEVTQDYIN
                     SDILEEYLLKRQRLMFKEILDRRIEKAGITKNILFSRIDNNLALKDFQFAYDDCISLL
                     NLWSDDEHVWTKTIQVCVESNDPERLKKVLDKTKKLNIQWSAANRALISTWQEGVQ"
     gene            complement(24787..26958)
                     /locus_tag="PLL88_04255"
     CDS             complement(24787..26958)
                     /locus_tag="PLL88_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ00807.1"
                     /translation="MNVLITGYLGSISPSLIEELSKDKENKLILAGEKIDEIKGRFPD
                     VISHDMDLTDPLFSEILKSSNLDVIIFIPLREEQLLSGEKQIAGRLLDILIKTLEHCK
                     NKTDIWYILISSTEVYEKIEIKQASNENHLLETALNKNILLTCEQYLQYYNQFFGLNT
                     SIVRVPFVYGSAETRSILRDIIESCQKEELVAIPASKDTVCGFIHSKDVADFVHRLLA
                     EEYYHEYKVFNLPPSDRISAGDLTHALGTYFPKVKFTFQKNKNFSTKSIEGILSRQEF
                     DWTPTNTLHDSLEHIVRSVKKIPATELKGFDKIKQKISKHPNVLKWVEVILGGCVMQA
                     LIHLTGTFIQFKYIDFRLLYVILIGSAYGIRFALLASVIAGVSNLYSWYQLGLDWELL
                     VYHVENWLPIALYLISGSVIGYVWDKKENEINFTKKQVGLMQEKYAFLYNIFEDIRKT
                     KDKLREQLIGYRDSFGRIYSITQELDSLNANDVFLKALEILEDVMNNKSIAIYSIEKT
                     KQFARLEVNSAPMSQQISKSLNLSDFPKALDFINKGEIFDNKMLLSAYPSLIAPLMKD
                     EQPEGLIFIWDRAFDQFSMYYLNLFKVLSGLVQSSLVHAILFREANLEKWYIPSTRIL
                     EPALFKETLQIKTAMQDRNISRFQVLRIERGNLGWVEFDKILQKGIRTSDYAGLLSKE
                     EPVCHVILSQAEETNIALIVTRLKKLGLECKLIESSSIGNA"
BASE COUNT         7614 a         5760 c         6273 g         7506 t
ORIGIN      
        1 ttaaagcctc cagctatgtc atggaatacc tgtcggaatc acaagaggtg atgggatgcg
       61 ctttgaacga tccgctggca ttggcagccc ttttccagga tgacatattg gaacttcacc
      121 atctggtcat tgatgtggat accaattatg gggtcagcat ggggaagact tttgctgatt
      181 tttacaatta cgatcacaaa cccgccaatg gatacgccgc tttgggggtg gactctcgcc
      241 gttttgtgac gcatttttta gaacgaatga ttaaatttgt tcaataacaa atgtcatatt
      301 attcatatga tattaatact gaaccttgac aaatcttttt ttccttagta tatttgcgtc
      361 caatatgcaa catcgaaagc atgagaacaa accgacaact ttctacgact cccaacttgg
      421 aggagctgtt ctcgtgctga ttcggtgcta gaccaggtgc aggtaaagag aggctttcca
      481 agagcggaaa gccttttttg ttatctgcac ataggaggtg ccccggagag aggaacgatt
      541 aacataaaaa atcttgaaaa atgccagaaa aatgccaaag gagaaaaaga tgaaaaaagt
      601 caattttttt gtagctctca tgatcattgc cgctttactg cttgcttcct gctccaccgc
      661 agcatcaccc gaaggggtgg aagaaagcgc tggtgaagag gtgaaaagtg gtatggaagg
      721 tcaagtctac gagtgtcttg ggacggcaga agatgcgctg gtagaccttg aatgtagaga
      781 gattaccatc gctgttgaga atgcctatct tcctttcaac tatatctcat cagaaactgg
      841 tgaggcgggt ggttgggatt atgaagtatg gcctgcaatt tgtgaactgc tgcactgtaa
      901 accggttttt gtagaaaccg cctgggaggt catgatccaa tcggtagcgg agggactgat
      961 gaacacggca ggggatggcg tgaccatcag tgaggaacgc ctcaagcagg tcgatttctc
     1021 gattgggtat atcaatgtgg aacagcgctt gttggttaag aagggtgaaa cccgttttgc
     1081 cagcatagaa gattttgtcg ccaatccaga cctgattctg ggcactcagg cacagaccac
     1141 caattatgaa acggctgtgc aatatcttcc agaagatcgc attcaagctt ttgagcaatt
     1201 ccccttcgct gttcaggctt tgatcgcagg ggatgtggat gctgtcttaa ttgatgaaac
     1261 ggctggtctg gggtatacgg gcaccagtgc cgaagaaata gagttgatcg gtccatccat
     1321 ttcgagtgac gaacttggtt tccccttcac gaagggcagt gacctggtcg aaccggtgaa
     1381 tctcgcaatc caggctttga aggatagcgg gaaactggag gagatcaata tgaaatattt
     1441 tggtcctacg ttcacactca cctacgacga tgttcaataa gaatcaataa gaacaataaa
     1501 tagaagtccg ccagaccggc gaatcaaccg gtctggtggg taccctagat tggagtagta
     1561 gatggcagta aatgatcgat tggtgacaca aaagaataag aatggattgc aaacgaccaa
     1621 cgagatgcaa ctgaccaaaa aagattttcc atggtggatg gtcgcgattg ggctgctgat
     1681 tgtaattacc tttttgctga tccggatcaa acccgatttt aaagatgcgt ttaacatcat
     1741 ccgggcaggt atgagtgtga ccatttcaac gaccctcatc gccttcacca ttgcgacgat
     1801 gttgggtttg tttattgggc taggaagggt ttcggagagc attttttgga gaaatgcttc
     1861 taccatttat gttgagatca ttcgcggtat ccccctgctg gtgctcatct tctttattgc
     1921 tctggtgttg atcccgggat tgaccaacct gctgagcgct ctcggcactg ttctgatcga
     1981 tgtggggatg gggagtttcg gcgcaaagct ggctgccatt gaaaacaaat ctatatcgat
     2041 gaacgtgcgc gcgatcattt cattggcaat cacctacgcg gctttttcgg cagaggtctt
     2101 ccgcgcgggc attcaatcga tcgataaagg gcagatggag gcagcccgtt cgcaaggtat
     2161 gagtcgctgg caggcgatgc gctatgtcat cctgccgcaa gccattcgga atatcctacc
     2221 ggcacttggc aatgacctca tttccatgtt gaaagattca tcgcttgttt caatacttgc
     2281 cgtgcgcgat attacacagg ttgccagatt gtatgctggt cattctttta aatttgcaca
     2341 ggcttattcg attctgtgcg ttctctattt aaccctcacc ctggttcttt ctttgtgcat
     2401 gaaggtgatc gaaaggaagt taggaaatga gcgaaaataa ccgccctatg atcgaaacag
     2461 aaaatctgtc gaaaagtttc ggcacgcttg aagcggtgca cggggttagt ttgaaagtgt
     2521 accccaagca ggtgctggtt atcattggtc ccagcgggtc gggcaaatcg actttgctgc
     2581 gcaccatgaa ttggctcgaa cagcccagcg agggcgaggt ttacgttgat ggaaagtcgc
     2641 tatctgaaaa ccagcaccac atcaatgata tccgttcgaa catcggaatg gtgttccaga
     2701 acttcaatct ctatggtcat ctaacggtca ccgaaaatat caccctggct ttgcgcatcg
     2761 taaagaagat gaagaaagaa gaagcgaaaa aaattgccat gcggcagttg atgaaggtgg
     2821 gcatagccga caaagccgat cgttatccct ccgaactgtc gggtgggcag caacaacgcg
     2881 ttgccatcgc acgggctttg gctatggaac cgaaagtgat gctcttcgat gaaccaacca
     2941 gtgcattaga tcctgaaatg attaaagaag tattggatgt gatgcaactg ctggtaaagg
     3001 aagggatgac catggtgatc gtcacgcacg aaatgggttt tgccaaagct gcggcagatg
     3061 aggtcatctt catggatatg ggaaagatcg tggaaagggc agcgccggaa gaattttttg
     3121 aacatccttc ttgtgagcgg accaagcaat tcctctcaca aattttaaaa cactagctgg
     3181 ctgatgctgg tattgaccga tggtggttta tttactataa tacgccgtcc aatagtagga
     3241 caatgtccta aattacagga caacaggtgt tgaatgcaga acggatccta tcacttctac
     3301 gaagagaaaa ttgttctacg ggtgcgaaac cgcgtttgca ataattcgga agagttgata
     3361 caaagtgagt tatttgcaaa ggtcttgcaa tgctttctgg aggatcttgc gcagcgccaa
     3421 tcgccgttgc tgaccgtttt tcctgattcg cacatcagcc atgactcggt gaaaaagttg
     3481 accgaagttc tgttctattt gatccacctg ccggctgcga aagtggaaaa gatcgttgaa
     3541 ggatcagccg ctttcctggc agaccctttt ctgttgaatg aattcgtcga gcagctctat
     3601 aattactggc gttccctcca ccgacttatc atatgtgact caatttttga ccgctttgac
     3661 caaaaaccat accggacgtt caatgacttg gtggaaagcc tcatgcatat tgtgcgctcc
     3721 acttaccgca ccattcaaga gaacatcacc ggcaatcacc ccaagatcta ccgccaggtg
     3781 agcgccggag cagaggttgg ggcgatcgcg ctgccttacc acatcaacta tccggatggt
     3841 ctgtacgatg tgctgcaaga tgtgttcatc atccgccagg cgttgatcta tcctcccatg
     3901 atcttcaaga cgcccatgaa caagcgcaat gggcaattcg aacgagtgga tcaaaatcca
     3961 ctggaaaaga tccatcttga acacgctgaa tggctgtgtt accccgccaa agtgggcgac
     4021 ctgctgatca tggtctattt ttctttggat ctgttcgagt tgggtttttc tctgtgtaat
     4081 ttattcgaac tggcagaaga ggatgacctg caacgcaagc cggatgctgt gttcctttat
     4141 ggggttcctc ccgaatgtgc attgcaggtg catgggaatg aaacgatctt ttatgaagat
     4201 actgaaaatg accttctgat cggtaccgtt ccaaacaagg atgaatttgg gtattttggc
     4261 tatcttaaaa agatgatcct gaccctgcac aatatcaaac gcatgcgcac tggctcgttg
     4321 cccttccatg gcgccatgat tcgcattact ctcaaagaca gccagccgtt cgccctggtc
     4381 attttgggtg attcgggggc gggtaaatca gaaacgattg aagcgttgcg acgcatccgc
     4441 accaaggaca ttgaagaaat agtgattgtg gcagacgata tgggttcgtt ctcgatcatg
     4501 cccgatggca agatcgcggg gtatggcaca gaaatgggcg ctttcgtgcg gttggatgac
     4561 ctgcaagccg gttatgcttt cggtcaaatc gatcgcacca tcatcatgaa ccccgaccag
     4621 gtcaatgcgc gggtggtcat tcctgtaacc cgttacgaat atctgatcaa aggcattcct
     4681 gtggatgcta ttctttatgc caataattat gagtcggtga atgttgacca tccttccatt
     4741 gaagaattca aaacgccgcg tgacgcttta cgggtatttc gaagaggggc tgtgatgagc
     4801 aagggtacaa ccaccactac tggcatcgtc gaaaattatt ttgccaatat cttcggtccc
     4861 gtacaatacc aggaactgca tgacaagatc gccagcgact attttggagc tttttacgct
     4921 gcgggcatct atgtcggtca aattcgcacc atgctaggca tctgcgggca ggaacagcac
     4981 ggacctgaaa atgctgcaat tgctctgctc gatctgatcc gcagcagggc atgacctggg
     5041 cgcgcgatgc ccattgtctg taatccagat tccgctgttc gtcctataat ggtcttacgg
     5101 atgtactaaa gatcatgaac ctggcgacgt ttcgctttta tgcggaattg aatgattttc
     5161 ttcccgtcaa atggaaaaag acgaatttca cgctcccatt ttcccgcggg cagactgtga
     5221 aacacatggt cgagtcgtgc ggagtgccgc atacagaagt ggacctcgtt ctggttaacg
     5281 gcaatccagt accttcttcc catcaattgc aaaacaatga tcgggttagc gtctatccgg
     5341 tgttcgagtg cattgatatt tcaaacatat caatgttgtg ccccaaacca ctgcgccatc
     5401 cgagatttgt cgtcgattgc catctgggaa ggctggcggt attccttcgg ctgttaggat
     5461 tcgatgtttt atacagaaat gattttcctg atgatgaact ggcagagata tcggagaaag
     5521 aaaaacgaat ctgcctgacc cgcgatcgag gattgctcaa aagaaatcag attgagcatg
     5581 gttacctggt gcgggcgctg gatccaagag cgcaattgat agaagtattg aagaggtttc
     5641 aactgaacct gcaattgaaa ccgttcactc gctgtacgcg ctgcaatgga tttctgcgac
     5701 cagtggagga agaagagata tcgagcaggc tgctgcccgg aacgcgtgaa catttccatg
     5761 aattttcgtg ttgtgagagc tgcggctgga tctactggcg gggagcgcag tatgaaaggc
     5821 tgcaaaagct cgtggatttt gttttgaaag agttaaaaat ggaacgaaac ggtttgggag
     5881 cgttgaaggt agagaatcat gactgatcgt ccattcaatg cgcatattcc aacgtgatcg
     5941 catttctttt catccatctt gcgattttaa gaattggtag tatcatttcc cccctgtgct
     6001 ttatgaactg tagattgttc tgaaagaatg tatcattaag gaagatgtga acgcgttaca
     6061 aattgatcga aaaaagcttg cgactctgca agcgaaagat atataccctt tgctggaatc
     6121 tttacctgat ggattggcga gtggggaagt gcttgcgcgg caaaaacgat tcggtctcaa
     6181 tgtattgcaa agcgagaaga agacccctct cgtgataaaa ctcctggcga attttaccca
     6241 tctcatggcg gtactgcttt gggtgggcgg aatcgccgcc ctagttgcac gtatgccaca
     6301 actggcagtt gcggtgtgga tggtcaacat catcaatggg gcgttcagtt tttggcaaga
     6361 attccgtgcc gagaaagcct cggaagaatt gcaaaaattg ctcccgacat acacgcgggt
     6421 gttgagggaa ggcaaagaag aaaaaatcct ggcagaggaa ctggtgccgg gcgatgtggt
     6481 gttatttgcg gaaggagaaa aaatctccgc tgactgtcgc ctggtcgaac aagctgcttt
     6541 tcgggttgac caatccacat tgaccggcga atcgcgacct gtcggcaaaa gcgctgatgc
     6601 ttcaaccatg gaagaaatta cccttacaga acaacccaac ctggtatttg cgggaaccag
     6661 cgttgccgcc ggcacggcga aaggcattgt gtatgctact gccatggaaa ctgaattcgg
     6721 caaaattgcg catctcaccc agagtatgaa ggaggagttg agcccgctgc aaattgagat
     6781 gaaatccgtc acgaaaacca ttacgatcat tgccatcatg gttggtatcg tcttctttat
     6841 ccttgctttg ttcctggcaa agatcgatct ggcggaaagc tttgttttcg gcattgggat
     6901 gattgtggct tttgtgcctg aagggttgct cccaaccgtc acgctggcat tggcaatggg
     6961 cacacagcgc atggcaaaac gacaggcgtt ggtaaaaaaa ttatctgccg ttgagaccct
     7021 tggttgtacc acggttatct gcactgacaa aacaggcact ttgacccaaa atgagatgac
     7081 ggtgcgtcga atgtggatcc cgcaaaaaga aagcaaacga gaattccatg cagatggaag
     7141 gattgaagta agcggaattg ggtataaccc cagcggtagt tttcattgga aggataaacc
     7201 agcctcgagt gtgcaggacg aggatgtgca agagattctg cgagcaattt tgttgtgcaa
     7261 caatgcccgg ttgatagccc cgcagggaga ggaaactgcg tggaaaatcc tgggcgaccc
     7321 aacggaagcg gcattgaagg tggctgccct gaaaaacaaa atgaacgaag aggaagaact
     7381 gcaggcgcat cctcgcatcg ttgagatccc ctttgactca agacgaaaac gtatgtccac
     7441 ccttcatctg gaacaggcgc atggattgca gggtgcgacc ttcgttcaag caccccgtgt
     7501 cgcgtttgta aaaggggcgc caaaggaatt gttggacctt tgcgcccaca tatccatctc
     7561 cggtgaagtt ttcccattat ccgaagaaca aaaacaggct gccattctgg agaatgacac
     7621 catggccagg gagggtttac gcgttctggc agtggcgttt cgcaggttgc ccaaggactt
     7681 tggctcacaa agcgcagagg acatcgagcg tgacctgacc tttttaggtc tggtggcgat
     7741 gatggaccca ccgcgtgaag agatcagcgc tgcggtgcaa aagtgcaatc aagcgggcat
     7801 ccgcgtcatc atgatcacag gcgattatgg attaaccgct gagagcattg cccggcgtgt
     7861 tggtatcgtg cggtcagcga atccaaggat catcactggt tttgaactgg ataaactgtc
     7921 ggatgaagaa ttgaaagatg cctttcaaaa tgaagtcatt ttcgcgcggg tttctcccga
     7981 acataaactg cgcgtggtga tggcgttgca gaacctggga catatcgtgg cagtgacggg
     8041 tgatggagtg aatgacgccc ctgcgttgaa acgagccaat atcggcgtag ccatgggtat
     8101 cacaggcagt gatgtagcca aagaagcggc tgcgatgatc ttgctggatg acaactttgc
     8161 ttctattgtg aatgcagtag aggaaggtcg cgccgtgtac gcgaacataa aaaagttcac
     8221 cacctacatt ttcacgagca atacccccga agcagtgcca ttcattctct acgcgtttac
     8281 cgccgccagg atccctctgg ctttgaatgt gatgcatatt cttgcggtgg accttggcac
     8341 cgatatcgtg cccgccctgg cattaggagc cgaaccccca gagccgggta tcatgcagca
     8401 acccccgcgc gatctgaaca cgcacatcat cacacccaaa cttttaaaaa gagcatacct
     8461 gttgttggga ccaatgcaga gcctcgccag catggcagct ttttactatt attattggac
     8521 gaatggtttt gccggacaat ggatggattt acccgctacg ggtgctcttt accagtctgc
     8581 aactgctatg gcgctggctg cagttgtaac aacgcagatc gggaaccttt ttgcacagcg
     8641 cgcagaaagc actcctattt accagatgcc gcttttcaag aataagatga tttgggtagg
     8701 tattctgacg gaacttgtcg tgattttcgc gatcgtttat gttccgttct tccatcaatt
     8761 tattgggacg aacagctttt cactgaaata ttggctattc ttgtttgcat ggataccctc
     8821 attgatcatg gtggatgaga tcagaaaatg ggttgaaagg aaaagaactt taaggggatt
     8881 gagcaaagga ggtgtgcgat gaaagtgatt gttattggtt gtggcaggtt gggcatcaac
     8941 attgcacgtg aaatggatca gcgcggcaac gaagtagttg tgattgataa agacgcgttg
     9001 acttttgaaa atcttggaaa gacttttcgt ggcagcactg tggtgggcat cggtttcgac
     9061 cgcgacattc tcgcgaaagc aggggttgat ttagcggacg cactggttgc ggtgacttcg
     9121 agcgatgaag ccaacgtggt gactgcgcgg attgccaagt ttgtgtttcg agtgcccaaa
     9181 gtagtggcgc gcgtatacga tccacgcaag gcagagatct accggcggtt ggggattcct
     9241 accatttctc cggttaccat ggcaggcgag aggatatctg agatgctttc attttcgcag
     9301 ttgaagactg tcatgaccat cggttctggt gaggtcttga tcaccgaagt ggaaatgacc
     9361 gcccctttcc agggaaaaaa gatggcacag cttgaaattc ccaatgtggc gcaccccatc
     9421 tcattgacga gagatggaaa gaccatgatg gcaatgccca atcaacaatt ggagatgggt
     9481 gatgtggtgc acctggcggt ggtggcaagt atgaccgacc gtatcagaga aatgctgaaa
     9541 tagtgttgga ggaaaccatg aaagtattaa tcattggtgg cggtaaagtt gggacctacc
     9601 ttgcctcaca tcttttggat ggcagccatg aggtgaccgt tgttgaacca cgacgtgaag
     9661 aattggaacg cttgcagagc gagttggggg cagaacatgt gatcttgggc aatggaacgg
     9721 acccggttgt gcttgaatca gccggaatac gcaccatgca ggtggtagct gcggtgaccg
     9781 gtagtgatga gaccaacctg gtggtgacca accttgccag atttgagttc aatgtgcccc
     9841 gcattatcgc gcgcgtcaat aatccgaaga ataactggat gtttaccgct gatatgggcg
     9901 tggatgtggc attgaaccag gcggatctca tggttcatct cattttggaa gaaatgtcta
     9961 tgggagatat gatgaccttg ctgaagcttc gccggggtaa gttctccatc gtggaagaaa
    10021 aacttgcgcc gcaatccagc ctgactggag cgcggttgga acaagccaaa ctgccgcaag
    10081 atagcgttct cctggctgtt atgcgcaaag atgatatttt gatcccgcac ggcaatttgg
    10141 agctgaaagc cggtgatgaa ctcattgtgc tggtgcatag cgctcaactt gaccaattgg
    10201 aaaaaatcct gaattcataa gcagaaaatc tttcagctat cccctgggtg gaatgaccag
    10261 gggatagctt ttaaaaaatc ataccaaagt cttataaatt gtttttcgga ttacgatatg
    10321 ataatgctgg aatcatcatc actcgtaaag ctgtttctag aaaggtgtgc ccaccgatgc
    10381 acaataccag ccgaattggc gttgtttttc tgcttattgt cgtctctgta gtaagcggtt
    10441 tcttgggagc atatattggc ggaaatatgg ttgccgcgaa cctggcagca gatgcagaag
    10501 aacaggtgat ccccacgcct accttgaata cgggtgaagt tcaggttgtg aaccaaacca
    10561 tcacgatcag cagctctgag atagacaccg tgatcacaga tgtggtggaa cagatcgaac
    10621 cggcggtggt cactgtggtg ggaacaatcc ccgggcaaca aactttcttt ggtcggacag
    10681 ctgattctga ggtgagcggt agcggtttta tcatttcgtc ggatggatat attgtcacga
    10741 acaatcatgt ggtcgaggat gccagtgcgc tttctgttgt gctcagcgac ggtatgcaat
    10801 tgaaagcgga gatcgtgaac agagatgtct atgctgactt ggcggttcta aaagtagaag
    10861 gtgccatgcc tgcggttgcg gcgctgggca gttcggcaga tttgaagccc ggcgaaacgg
    10921 ttattgccat cggatcgccg ctgggaacat tccgcaacag cgttactgtt ggggtggtga
    10981 gcgcaaccgg gcgcacgctc gatacagggg aagggtataa tatggaaaac ctgatccaga
    11041 ccgatgcagc gatcaacagt ggcaattcgg gaggtcccct ggttaacctg gcaggagaag
    11101 tgattggggt gaatgccgtg gtagtacgtg gcagctccag cagcagcgct tctgccgaag
    11161 ggttaggttt tgccatccct tcagacacgg tgaaggtgat cagtgagcag atcatcgaaa
    11221 agggatattt cgcacgcccg tatttgggag tgggaatcca gaatattacc gcatcgatcg
    11281 caacgcgtta taatttaccg gtcgcatggg gcgggtatat caccgaggtg gtcgccggca
    11341 gcccagcggc aggcactggt ctaagagcgc atgatattat cacgcgcctg ggtgatatcg
    11401 ggatcacgga gggtaccact tatcttaatg cattgttcac ttaccaacca ggtgaagaga
    11461 ttgaagtgga attctatcgc ggctctcaaa agatgacggt aactgtcact ctggctgaaa
    11521 cgaccgctca ataaattcat tattaaagga aaagttttga ccatccattc catcgagccc
    11581 atccgcctga tctgttcaga tgtggatcat acgttattga ctgaagaaca acagcttctt
    11641 cccatgaccg aagcaacgct gcgaaggttg caagagcggg gggttaagat tatcctcgca
    11701 accggcaaga atctgggcgc tacccgtgcg attgctgatc gtctagagtt ggatgatccg
    11761 ctgatcttcg tcaatggcag cctcattcaa tatcgcgacg gaaaaatctt cagtcagcat
    11821 gaactacaac ccgaaattac tgaacagctc attcaactgg gtgagagcca gggcatggat
    11881 atgatgttgt atgtaacaaa cgatattttt gtcaaagagg gcggaaagta ctcgcacgct
    11941 ctggataaat atggcggtcc aaaaccgatc gaactcaaag actggcaaga tatgggtgcc
    12001 aacctcgctc atatcctgaa gttcgtcttt attgatgagg atttcagccg gttggaaggg
    12061 atgaaggtga tccttgaaaa aaatatttcg aatggaatcg aactgtgttt ctcattgccg
    12121 atcctgttgg aagtgcaacc agccggggtt agcaaaggat cggcgcttga aaaagtggca
    12181 aaattgctgg aattacctcg cgaagccctg atggcgtttg gggacggcaa caatgatatc
    12241 gaaatgctgc agtatgccgg aattggagtg gcattggaga acgccactcc agagttgaag
    12301 aatgtagcca gccatctggc tccttcgaac aatgaggaag ggctggcacg atttttacag
    12361 gattttttta atttgcagtg atcactgcct ctggtttatc ctctggaaaa gccagcagca
    12421 agataatgcc ccctgctagc aggaagggca acgcgctgat caccccggtt ggatacggcc
    12481 ctaacagggc gggcggattc ttgaccagga acaatacccc gattgatgca ataccgttca
    12541 aagcgccgtg cccaatgacg gcaggccaaa cgctctctga tttcaaagag acccatgcca
    12601 ggaagatacc gaccagcgtg gtaaaccaga ccattgccag gggtccgagc cagggaaaac
    12661 caggatagtc aaacccgtag ttatagccca tagcgatcac cggccagtgc cagacacccc
    12721 aaatcagccc ggtcaacagg atcgcctttc tttttccgag tggcaccaac tttggcagca
    12781 ggtatcctct ccatccaaac tcttcgccaa aggtgctgat catattcaga attggggaaa
    12841 cgaggatcgc atatgcaatt tgcatgataa tgaactggta tataaaagaa gcgctggaat
    12901 cagccccgta agctgccaat tgggaggaaa gcacggtcaa accggaatcg aaataacttg
    12961 gaaaaagcac aaaaaagaga gcactcccca gcaaaaccag cacaggtatg cctacccagg
    13021 ctgccagaac ataccgccaa ttcgcgcgga aatccaattt caataaaagg gtaccttttt
    13081 cttctttggt aacggcgcgc gtaaggatat tggcgatggc tggtccccac atgtaggcgc
    13141 ttgcaagcaa gatatatgcc aatgaaatgc cgctctcttc catgactatg gggctgttgg
    13201 tcaaccctcc cgtcacataa atgaccagag cggtcagcca ggaaatgcca aaggcaaaga
    13261 gcagaaataa tttggtcctt cttttgttta attcatccat aatacaatcc tttctagcga
    13321 gtgttctact cttagacctc gatcccgaac acgcggcgaa cgaggtttcc gatcaagaaa
    13381 gagaaaagtg cgacccccag actgatcagc gacatttcaa agaaacgttt tttgaaatcg
    13441 aatccttttg ccacagaaat atagaagttg aagatagcga tcaccaggat ggcgttggcg
    13501 atggtccatg ccaggctgac cagaaaattc ttggcaacca gataggggaa gatcagaatg
    13561 gcgaccgtca gaatgtaggc gaagccggtg taaatggctg atttcaccgg cgacttttca
    13621 ccctcttcag attttgaaga gagatattcg gaagcagcca ttgagaatgc ggcagagatg
    13681 cctgtgatca ggcctgccaa cgcgatcgtc tgggtgttct ggaaagcgaa ggtcaagcca
    13741 gccagggcgc cggtcagctc aaccagggcg tcgttcagcc cgagcactac agagccggca
    13801 tatttcaagc tttcttcatc caacatttct agcagttcgt tttcgtgttg ctcttcttcc
    13861 gccagaatct gcctggcttc ggggatgaga tctactaatt ccttgtaatt ctcctgggct
    13921 ttttcttctc ccagttccat cagtttgacc ccgaatgtca gacccaacac cttcgccagc
    13981 cagtaataga attggactcg ccattttttg ggtttggttt cgcgttgcgt atagcgcttc
    14041 cagacttgat aatgcttcat ttcctcctcg gcaatgcgct ggagaacttg agcgttgtgc
    14101 tcgtctttaa cggcagtcgc cagtttcaaa taggtgtaat gcccgtctaa ttcctcttgc
    14161 tgtaaaacaa gaatgcggtc tttttgtgcg gtggtgaggg acatagtgga cctcctttcg
    14221 tgggtgtaat tgtactattt ctattggctt ttcctgtgtg atattttcaa atttattatg
    14281 gtggaaatct tttcaagagc caatgatgac accgatccgt gaaaaaattc ctgttaattg
    14341 aggacggtga ggggtgggac agggtttgag gataaaaaac gatttgaacg acggacagct
    14401 catctatcag gattgatgat tcatcaatag tgctgggctg cttggctcgg caaatctgca
    14461 atacgtgtgc aatatgtatt cagacatgat attttgaaaa tattatcaat aagcccggtg
    14521 cggtgccgga ttaactgaaa tttccttttg ttgctgaccg aaaaactgtt gatgaaaaaa
    14581 ttatcgatct atttcaaagg aagcaagaat ttgaatggaa tcatcgcgac tatttgccat
    14641 cgcaaattga actgcaggta gattggtttt tggatcgtgg atcgcgattc caagagagta
    14701 tctgccattt ttcagtccct cgacagataa gtcgtatttc accacggaac tatcgcctgg
    14761 aaggatggaa cgaacgtcca tttcgatgga aaaaaaattg acaaggttgt tttgatcgtc
    14821 aaaaagatat aaaaatacgg gccattggta atacatggga gccacgcctc cattcgaaaa
    14881 ggttatgacg attgtggctg gtcctttcgc atgaatcttt aatggcaatt tgattttttc
    14941 tatataaaga cggtagccga tgttggataa aacctgatca actccctcct gcaaagaacc
    15001 atcttcttcc ccttcataaa ctccattcgg accgatgaat gtagtatgcg attgcctcag
    15061 cagttctatg gtctgggaca gtgaagtgaa atacacttct gtgtctttca atgaaggtgt
    15121 ctgctcacca ccaatcggcg cgatcttcca ttggtctggc aaagcgacca gtgcgttcga
    15181 ttctcctgtc tggtcaaagc ttccaccatc cgcaatccac cccaaccatg ttatcgtact
    15241 ggaataatca gcggtcatgt cattgtaaaa accgatctta ttattttcag agattgagaa
    15301 gggtctgcgc attaacaaga aggtattggg gaaagcagcc aggtaatggt tcacataaat
    15361 atctcgaatt tccgtttctg gcagagaacg aatgcctgaa tcactcttaa cgtgccattc
    15421 tccccagtga ccgatgcttc cgagttctat ataagcgatg aaatcatctt gtgcatatcg
    15481 ttctcccagc gctgcgatcg tcttttggtg gtattcgata agtattggat tagcatagtt
    15541 tggggcaaaa cctttaccat agtcgctatc ataaaaatct ccatcgccat tgatttttgc
    15601 atataaccag tcagggatat ccatatgggg ctcagagccg ggcacatcca aaacaaaacg
    15661 aaaaaccacg cgtttccctt cagccctcca acgctcgaat tgattcttca attcaaaaga
    15721 tttgaaatca aaaatgccct cttgaggttc gaattcacgc caggtgaggt cagcatatac
    15781 cagagtgtag ggctgctcgg attcttgtat cgtagcccag ggagcccaac ccataagagg
    15841 atttgcgatt ggatcttgtg tcatttcaaa ggtttttacc tgttggagtc ctgtgtatag
    15901 aatccaaagt attaatgcaa cgagcacaac gacaattaaa atattttttt cgcatgaact
    15961 tttttgtcca ttttgtcgct cgagagcatt cattaattga aatattcgcc ctcgtactgt
    16021 acgagcgtta catcagtgac agtaggcagt tccttgatgg cttccataaa ggcaaagttc
    16081 tcattgcgta ccacgagctc aattgcaatt tccactttct cctgacgcat cgttttcgat
    16141 ttaatttggt agcgattttt tctgagaatc tgcttgagtt cctcttcggt gtcactcatg
    16201 gagtagtgaa aaagcaatat atacattttt ttattgctct tttgtgaatt gaggaccagg
    16261 aataatatga agacaatcac cgctcctatc agagccagat aatgcatctt tgccccgatg
    16321 gtaattcccg tcgcgacacc ccagaatagg aacatgatat caatggggtc tttaatggct
    16381 gtgcggtatc tgacaatgct taaagcaccc accatgccca gtgaaagcgc aatattagag
    16441 ctgatggcta aggtaataat cgttgacaaa atagtcatcg cagccagtgt tattgagaac
    16501 ccgtgattat agataatgcc tttgtagaag cgacgatata cataaaaaat gaagattccc
    16561 agaagaaaag aaaagatcaa agacgtgagt tctggcaata aactgtacaa cgcatctaca
    16621 agatcatttt gaatgaatga aataatcgta ttgaacatgc tataactcct tttattggta
    16681 tctttttact cactcgttct tcgtttgaac attgttttat tacaacagat gacgtatttg
    16741 gacatggacg tatataccgc gtggggagcg ggaaggatat cttttattat ggtgggtaaa
    16801 aaattcgtgt atttcacttc caagatcaga taatttggtt ccagggcttc aatcatagcc
    16861 atttggtgat taaaaatatt caacgcttcc ataccagagc gtatatttct atccaatgta
    16921 atcctgacat caccagcttc cataataaat ggctcacgct cataatcaac aatcacacgc
    16981 ggtcgcagaa aatgagatgt gcacatgtga taaaagatca tttttaactg cgaatcgctg
    17041 tttcttaaag cgacaaaatt ctcgtccaag attgcttcaa cctctgatct attgagaggt
    17101 gcgatttcct taaaaacgta attatttgac ttgacttttc tttccaagtg gatcacttga
    17161 tcagaatggt tgtatagccg aatacggaat ttttcacgat ctaaaatgct catatccttt
    17221 tcgttatatg cagtgttgaa atagtcatca aaatataggc ttcttactgt ataaaagcct
    17281 gaggggcttg cgtgaagatc cttctttaat acttctttca agcgcaattg aaggagcgaa
    17341 ccatttgctt cgtcgattat gtactttaat tcgtggcgat aagccatatt atctgcccga
    17401 agtgatggtg atattttgat tctcttggtt tatgtaaaaa tttcttatcc ctgggtgctc
    17461 cactccattt agatcggtat aataatcaaa aggatactgc attttcccgt cctcgtttac
    17521 ggcgataatt ctccaaaaat attcacctgg aggaagaagg tttaactcga cacttaaaaa
    17581 attgacaact tctgtttgaa acacaatttc acgaaaattc cagttcttgc tgatcgagag
    17641 ctgataggtg atattttgtg cgtcaaaatc atatgcttca tcccaactaa agattatcat
    17701 gttgttgatt atttgtgggt cacccaagaa aaaaggcatg ggcgattcaa gggaatctaa
    17761 ataaagctgg tagtttatct gaatttcatc aggcagaata tccaggactg tattgtattc
    17821 atcaattgtg ccgggtagat aaagaatatc tggcattttt gcaatatatt ggtcagtaac
    17881 aattctgtaa gtggcaatag cttctgcgat gcgttctgga gcgaagtaag ccatcagttc
    17941 gtttattttt ttatcaagct gctctcgata ttctgctttc gataagattc tcttatgtag
    18001 aacacatccc cagtaatttg aaattccttt ttcgaagtag agcggattag atcttcccaa
    18061 gagctgtctt tcttgccttg ccaatatccc atcgtaatcc catgcaataa agtaccattt
    18121 ttgactattt tgtgggctat ataagtagaa attttgactg tttgtatcaa ggtttcctat
    18181 aaggatatta aatgccatcc agataaagta attttcggca tcgaaatacg tttcaaagat
    18241 ctgttcaatg ggtatgctca aattatttac gtcatccagc atttgaatga gttttgaatg
    18301 gtcttgattt cccttaattt ccaacacagt ctcgaataaa tcccgattat aaaggggatc
    18361 atcggggagc cgaagggtat ctggatatct aaagaattcg aacatatttg ctttataaag
    18421 ttgaccatct tgatcaagaa ggtgattgcg tagaaatttc ctgttgggca attcaacttg
    18481 tgtaaataat ccgtaatcca taaaagtatc actgggaggg gaggttgttt catctttgat
    18541 aaacaggttc acatactgag tgcgtaaact gatgagtcca tcgagatttt ccatcaattc
    18601 aaaacccaac ttattgcgca tccttgtaag atcactttga tgtttgttta aagctatcgt
    18661 cgattgacct ctccattccc ctgcgctatc aaaaagttct attttgtagg atttttgtgc
    18721 ttccaaggat gaagtattcc ctctaacttg aatgatggca ttggggacta tttcaccata
    18781 tccaaattgg ccaggcaagg gaccattttc atctccaatc tgaagaatag cttctgcctt
    18841 atttacggac gtgtttattg ttccctcgac aaaaaatttt gatgaatcat tgacttcttg
    18901 ccaggtatga tcggtatcat cacccgaatt tcccctgcga actgtaacat acatatacac
    18961 gatagaatcc ggatcatcat ttaaataaat gtccgggtta tccttgagcg gtagatcgtt
    19021 tatttcagtc ggctgttcta tcactatttg ttcacgatca atgttgagtt catcttggat
    19081 gtcaatttca acttctggct cttcaagatt tgcctctaag ctttgaaatg agataagcag
    19141 agcagccaaa attccgatga taaccaatag ggatcgaaaa atctttttca attaactctc
    19201 cttgaacaat taacaacgct gagaaagagc ggtattaatt attcttcaca aagattggct
    19261 gtttgcaaaa aatgaaataa tccagtcgcc ttgtatagaa agacaaacgg ctcaatgtga
    19321 ccaaacacat cgtcaaacct gctacgagaa atccgaatcc ataaaaatta gatgaaagtg
    19381 agattgtata gagggtgcca attgtattta ccagcagaag cgtgaacccg gaaaagactg
    19441 cccctatgaa atccacaaaa tacaaaagga ttagcatcat gctgttccct attgcataaa
    19501 aagcatatcc aacacacatc acgcgataaa gcccgatcat ttcgtgtgta aacccaagcc
    19561 cgagattact gatgatccct gatacgatga caacaaatat gatcgtagcg aacatttgcc
    19621 gaagggcaag atagaacagt tcttgtttca gtacaattaa catttctgaa tgagcttttt
    19681 taatgtcagt cagcgcccca ccattgttta tcaaaccaaa ataaacctga tacttgggat
    19741 aaaaatttac ttcgacagat gtaacgaaat ttaccgtggt aacgagaatg gataggaacg
    19801 caaaaagtgc tgggatatca tgcatgggcg catggtaaaa caatccgatc acttgaattc
    19861 cccatgggct tttccacatc agcatgagat ggatgaacaa tcccagagtc gtaaaaaagc
    19921 caaccatcga aagggaagga tattttttta tccatttaag aaattcaaag gtctctttat
    19981 gttttaatgc tcttaatgga aaatatttat gtagcgcgta gacatatacg attaccatga
    20041 tcccatatgc aatacaagcg gaggtaagaa aagcggttac cacttccgcc tggaacaata
    20101 agatcaatag cactccagcc aacaagccgg tgaaaatacc agcaataaaa cctgataaaa
    20161 tgtcacggta atatttcact gcggtgatat acgtgatctg tacccatgca accagcgctg
    20221 aacaaaaaag gataaaagat aatatggcat aatggaaagg aatatctgca agataaagaa
    20281 aaatgcccca acccaaggat ccgatcaata acaagattga ggtcaaccca tacattgagg
    20341 gcagaatatt cttatattcg ccttgataca atttatcggc gatgtagcga gagatcatat
    20401 aggaaaaaaa actggtcaac aataatggaa atagaagaga gtaagttgta attacgataa
    20461 ttacatcctg ctcatgactg cttgctccga attggatcga tagatacttt tgcccaaaaa
    20521 gcaataaggc gcccatgatc ataggaccgg caattacaat gctggaataa aaaacagccc
    20581 gaatttttga aaatcctccc cttgaagcgt acaatttttt taactcgaac ccaattcctg
    20641 ccattttatt tctgcctttt tatagacgct gaggtagttt tgaaccatcg cctcatgttc
    20701 atagaatttc ataacccttt tttgcccaat ttcacccatt ttatagcgca tttctttatt
    20761 ctggcacatt ttcattaacg cgtgcaacaa ttctgtttga tgcatgggtg gaacataaat
    20821 ccctgctgcc cctaaactat ctcgaccatc tccatcgatc aattctctaa ttgaaccgac
    20881 attggttgcg accagcggtc gtctggcagc cagagattca attgcagcaa aaggctgccc
    20941 ttcggagatg ctggtcagca atgtgaaatc aaattgcttt agataagatt ttacatccac
    21001 aagtccaaga aactttatat ccttcaaatc taagtattcg ataagatcta tgcattcctt
    21061 atagtagtct tcatcatcaa cgcttcctaa tatagataag cgtacatttt taatctcctg
    21121 ttttaatcgt gagaaagtgt agatcatggt ctttatgtct ttaatgggag cgatccggac
    21181 aatcgcgcca atatcaaccc aattactctc ttcttttaca ggaagagacg agtattcctt
    21241 gcaaaaaata ccattactga tcacttcaca ttttgttgcc ggacaaccaa tctcttgttg
    21301 aatatcattt gcccttttaa aaaggcttgt gacgacatgc gctgattgat aagcaaattt
    21361 ggagtacata tagaacatgg aaatccacag ttctttaaaa tgcggaacaa cccaatctgc
    21421 gcgtaagatt tcctcttctc tttccctggt ataaattcca tgttctgtga gaatgtatgg
    21481 tttattaaat tttctggcgc ttatacctcc cataacgcct gcatatcctg ttgatagact
    21541 gtgatagaga tctgcttctg gaatttcctg gctgatcaaa gagagaatgg gtaagaacat
    21601 ggaacgaacg gtccaaaaca agtcagtaaa gccgacgaag ggaaactcat cgagtgcgaa
    21661 ttcctttaac atctctagaa aagcgtgact tttaaataag ctgatggctt ttttttcatt
    21721 tccgccaaaa cagttgaaga gtatgtccca atcaggattc ttgcattgaa ttaattgaga
    21781 tagcgctgta atttcgtctt tactcagttt catgaaagat ccatgcacca tgcgagttct
    21841 tagtggtgaa tccagatcat tttcatagat atgaagtaca ttggaaggga tttgatattt
    21901 aaactttccc tttcttgaag gagagtcaac aattgtccat aaaataaact catttcgtgg
    21961 taatgctcga atgaatccat ctacccaggt tgaaacacct ccctgcacgt atggataact
    22021 tccctctaaa aacaggcaaa ttttcaatga tcactcctcg aaggcaattt taacctgcgc
    22081 actttctgcg ccgatcaaat aaaggcttgg cgtaacttcg ctgatcgttc caccaataat
    22141 attggttggg gtagaggatg tgcgcattaa caaccatgct tcatcataga agttgccaag
    22201 ttgaatggta caggtggtat tttcacaatt tgtattaagc tctaaccgag aaaaacgttg
    22261 aaccgctttc cctccctcac ttgcggtcat ttgtcttatt tcaggagccg ctccttctaa
    22321 ccatataaaa tattgctcga gattatctcg aaggtatccc cacccatatc ctgaacttct
    22381 tcgctcatca agcacatcat caggatgaat gaattgtgaa ttgacatagt gaagaaccag
    22441 ttcacttgct gcttcccagc gcatgattga tgcgaattca taaccagaga taatacgagg
    22501 cacctcaata attccatcag gtgcttcttc gaattcctga acataatacg catcagaatt
    22561 ctcatctggt agataagagg aggagatcac tttaatatca ggaaataccg tgggcagcca
    22621 tgtgcgcact tctggatcga ggatattcga agggggtaca taagtaataa attgctgttc
    22681 gggaaaaagt gattcagcaa atgttttaag cgcttccaat gataagtgga tcgaatcaaa
    22741 ggaagtccaa atagggtatc ctaattctgc tattgtttcg tctatgctta tggcgaaagg
    22801 aatgtgatta tagccgtgta atcctatttc tcccccaatc gataataaag aactaccaaa
    22861 ataagaaaat tgctcaacgg acgtttcttc aatgaaagag ggagatgtct tatcattgta
    22921 agtttcaata agaagcccag tatatttaag accgtatcta cgagcgaaat ccttcatatc
    22981 gggccaccaa atgttgatgt agaagctctc tatatctctg ccaaactctt gaaaaactaa
    23041 atcattgatt ccttcgggaa taggagccgg aaaatcatcg atgaagacca tagaactatt
    23101 tatgaccgga taaatgaacg tgtcgtatag gagactataa gcagcgcaaa tcatgcctcg
    23161 atcatattta ttggcaaatt ggtctgaatt gatcacaacg aatcgccctt ttccgaaatc
    23221 ataatcccat aaaatgggtg ttttgctggc atcaagactt cgtaaatgca cgtgtgcgct
    23281 tttctctaat tgcagaacga gacttgaatt gttcacagaa ccgacgtcca gagtttgtga
    23341 tttcatcccc ggaaaaagat cgctaatgaa ttcaatttca gagatgggtt gggtaccact
    23401 gtcgtcgtat gatacgattc ccaatttccg gtagatggcg ttaaaggact cggaaatatc
    23461 cggtctgatg gttatcaata aacgtccgcc attttcgacc cagcgtgtaa tatcaacgat
    23521 ttgctgactg atcgcatcaa gagcataaaa agtgatcacg atcgtttcat agttctcaag
    23581 ggctagtggg aaagtacttg caatttctgc aacattaaaa aaatcataac caacccgcat
    23641 gctatccagg gtgtctttca cgatggtaaa ttgctttaga tcgacatcgc tttccgaatc
    23701 atatagtacg aggcaatcat ttttttgttc actctctaca gggggctggg tcgttgtcat
    23761 gggattgaga aatgaaagag gtgaagactc agccttgtag gggattccgg atcgctcaac
    23821 aagaagccca aacccaatgg tggaaagaat taatattggt atcagtaacc aagaaaaact
    23881 gacggtgctg ttcttcattg aacaccttct tgccaggttg agatcaatgc tctgtttgcg
    23941 gcgctccatt ggatgttcaa tttctttgtt ttatcgagca ccttctttaa cctttcaggg
    24001 tcgttgcttt ctacacatac ctggattgtt tttgtccaga catgttcgtc atcagaccaa
    24061 aggttgagca gcgatatgca atcatcgtat gcgaattgaa aatcttttaa cgctaaatta
    24121 ttatcgatcc ttgaaaataa aatgttcttg gtaatcccgg ctttttcgat tcgtctatct
    24181 aatatctctt taaacattaa tcgttggcgt tttaatagat attcttcgag aatgtcacta
    24241 ttgatatagt cttgggtgac ctcaatatat gtgtctaaca attgtatgtc ttccggattt
    24301 ttttcaatgg ctacggatag cttttgaagc tctatctgaa aatctcgttg tatttttgaa
    24361 atggtggttg aagcataatg aacggtttca atatcatcat ttgttcttgc gaccagtaag
    24421 acatcaataa atttggatgg attatcatac agtgattcaa gcattaactt tcgacgtgtt
    24481 ttcgtatcat ttaaagaaat ggcttcttcc agagggatca agttgatgtt ctcttttcga
    24541 ttttcaatag gtctccagta aatgttctcg ccaggcgata attggtagat ttcaatagga
    24601 ttttgattat gcccttcagc cttgtaatac atgtcaataa tcaatgcggc taatactcca
    24661 aaaaaaggaa taaagaataa tatcgggatg aattcttttt gatggtttga tctgcccaag
    24721 agtatgaaga tcacgtaaac cataacagaa acgaaatgaa tgaataaaag aatgaataag
    24781 gtagaattag gcattcccaa tgctgctact ttcgatcagt ttgcattcca atcccaattt
    24841 ctttaatcgt gtcacaatca acgcgatatt cgtttcttca gcttgagaga ggataacatg
    24901 gcaaacaggc tcctctttac taaggagacc tgcatagtcg cttgttctaa tccccttctg
    24961 taagatttta tcgaattcga cccagcctaa attccctctt tcgatgcgta aaacttgaaa
    25021 tctgctgatg ttacggtcct gcatggcagt tttgatttgc agggtttctt taaataaagc
    25081 aggttcgagg atcctggtag atggaatata ccatttttcg aggttggctt ctcggaataa
    25141 aattgcatgt accaaggatg attgcaccaa tccgctgagg actttaaata agttcaaata
    25201 atacattgaa aactgatcaa aagcgcgatc ccagatgaat attaagcctt ctggctgttc
    25261 gtctttcatc agaggtgcga tcaaagaagg gtatgcagac aagagcattt tattatcaaa
    25321 tatctcgcct ttgttgataa agtcaagtgc tttcgggaaa tcagacaggt tcaatgactt
    25381 tgaaatctgt tgtgacatgg gggcagaatt cacttccagg cgggcaaact gcttggtttt
    25441 ttcaatggaa tagatagcaa tgcttttgtt attcatcaca tcttccagga tctccagagc
    25501 ttttaaaaac acatcatttg cattgagaga gtccaattct tgcgtaattg aatagattcg
    25561 accaaagcta tcgcggtatc cgatcaactg ttcgcgaagt ttatccttcg ttttgcggat
    25621 atcttcgaaa atgttataga gaaacgcata tttttcctgc atcaagccaa cttgcttttt
    25681 tgtaaaattg atctcgtttt ctttcttatc ccatacatat ccaataactg aaccagagat
    25741 caggtatagt gcaatgggaa gccaattttc cacgtgataa acaagcaatt cccaatccag
    25801 cccgagttga taccagctat ataggtttga aacaccagct atcaccgaag ctaaaagggc
    25861 aaaacgaatt ccataagctg accctattaa aatgacgtac aacaaacgaa aatcgatgta
    25921 tttaaattgg atgaatgttc ctgtaagatg gatcaaggct tgcatgacac atccccctaa
    25981 aataacctcc acccatttca ggacgtttgg gtgttttgat attttctgct tgatcttatc
    26041 aaagcctttt aactcggtgg cagggatctt ttttacactt ctgacaatgt gttcaaggct
    26101 gtcgtgcaag gtattcgttg gtgtccaatc aaattcctgt cgagaaagga ttccttcaat
    26161 tgatttcgtt gagaaatttt tattcttttg aaaggtaaat ttaactttcg gaaagtaagt
    26221 tccaagagca tgagtcaaat ctccggctga aattctatct gagggaggaa gattgaatac
    26281 cttgtattcg tgataatact cttcagccaa taaacggtgt acaaaatctg caacatcctt
    26341 agaatgtata aaaccgcata ccgtatcttt actggcagga atggcgacaa gttcttcttt
    26401 ttggcagctc tcaatgatat ctctcaaaat ggatctggtt tccgcagaac cgtatacgaa
    26461 aggcacacgg acaatagagg tgttgagacc gaaaaactga ttgtaatatt gtaaatattg
    26521 ttcacacgtt aagaggatat ttttattcaa agctgtttcg agaaggtgat tctcgttcga
    26581 ggcttgtttt atttcgatct tttcatatac ttctgtggaa gaaatcagga tataccaaat
    26641 atccgttttg tttttacagt gttcgagggt ttttattaag atatctagta aacggcccgc
    26701 tatttgcttt tcaccggaaa gcagttgctc ttctcttaat ggaatgaaga taatgacgtc
    26761 tagattgctg gatttcaaga tttctgaaaa taaagggtca gtaagatcca tgtcgtgtga
    26821 aataacgtca ggaaatctac cttttatttc gtcgattttc tcgcctgcca aaatgagttt
    26881 attctcttta tctttcgata attcttcaat tagacttggg gaaattgatc caagatatcc
    26941 agtaataaga acattcatga gccacctttt tatttattat aagcattaaa aaagaaaaga
    27001 atagttatgg tcaaaatgaa tgaaacacaa taacctatga caaacggaga ggcaaccctt
    27061 attcactgca tccctgtttt cttaaattat ttgtcaaggc caaatagaaa gaacctaagg
    27121 gtctaccaga aggacaggag taccgtcgag ttt
//
LOCUS       DAOSDS010000022        26670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_841_length_26670_cov_5.353259, whole genome shotgun sequence.
ACCESSION   DAOSDS010000022 DAOSDS010000000
VERSION     DAOSDS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 26670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26670
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_841_length_26670_cov_5.353259"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="PLL88_04260"
     CDS             <1..813
                     /locus_tag="PLL88_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ00808.1"
                     /translation="HILSEDEIAYIALHIGVLMEEKKAYSEKLKCIVLAPDYYTVGRS
                     LYRRLNEKFSDSILITNFITSYDDLASTEEYDLLLSTIEINPRTEKPHILMDPFLSDT
                     TISNVFQKIEEVKQAKRKQKMTNQFKYFFRNDLFFYDYPFKTYQDAIETMCDSMMEKD
                     YVDKNYKKEIYEHEEVAPSAYGNIAIPHPLSNRAISSVIAISINPTPIQWGTNKVHIV
                     FMLSLQEENRDLFTDIFNSITHLISNKDFFTKIMNAKTYEDFIEILVSSTVN"
     gene            1136..3064
                     /locus_tag="PLL88_04265"
     CDS             1136..3064
                     /locus_tag="PLL88_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ00809.1"
                     /translation="MKYSHLFEPLKVNTLILKNRVLLAPMGYADPVTLSEQALGGPSL
                     ITIGSAQIDEESSNIMNQPYPFSKGGLTRLREQVVTIRQAGAKASLELIHGGQFAGKS
                     YISSNYAIGPMALIREDGLEVKAMDEPMMYEIAERYANAAKEAQAVGFDMIMLHFAHG
                     WLPAQFLSPHFNKRTDQYGGSLENRARFPKMIIERVRSAIGKDFAVELRISGYEWIDE
                     SIEIGDVISFVKSVENLIDMVHVSAGMDGAGIDEMLSAHCHMMTTTFEPHMANVWLAE
                     RVKKAVNIPVVTVGAIMHPDEAEDILSTGKADAVAIARAVVADPLWSQKALEGKGEEI
                     TPCLRCLSCSHVATRRRNSWGCAVNPRHGREAFVSAVIEKAPMIKNVVVIGGGPGGMK
                     AAITARQRGHTVTLLEKSGELGGNIKFSEYDKLKLDLKLFKDYLIRTVKQSGAAIKLN
                     TEATPEMVREMHPDVIIVAVGANPITPNIKGIKIEKKNVMQSIEAYPNLEKIGKRVVV
                     IGGGYIGCEISLELALREHEVTIIEISDVLASNGNRLYKEALHQKMATCTGLQYHLET
                     VCTEIQDNRVVIQGKDGVEKRVETDTVILATGFKANRTVAEAFYGITYETYLIGDCER
                     ARKVDYAINEGYFIGRNI"
     gene            complement(3133..3306)
                     /locus_tag="PLL88_04270"
     CDS             complement(3133..3306)
                     /locus_tag="PLL88_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00810.1"
                     /translation="MFITVALLTLWIWLWIRFVIVKAFPFMDRVNQFLLGLLAGVVTQ
                     VLLSLFYWMVFVR"
     gene            complement(3460..4185)
                     /locus_tag="PLL88_04275"
     CDS             complement(3460..4185)
                     /locus_tag="PLL88_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOJ00811.1"
                     /translation="MAQNSIHIGLVIPRPVKDSATPLVELEQYTADAKPDIFLFPEDH
                     IYSENLSALQEIARSRKKWIVCGMEDRDAGKEKFKHAVVVNPSGEVVGHHRKTSLTYN
                     ELSKGFSHGDDLDVIATDFGLVGIAICYEVHFPEVARSYALQGARILFNPIGTGMWHE
                     TQFAQWTAAGLCRASENGVFAVGCSHYNDAIPLAYAYAPDGSCLVQTRDANRLVTVEL
                     DLTQCSEMFIEHRQPHLYGRLTQ"
     gene            complement(4258..4968)
                     /gene="deoD"
                     /locus_tag="PLL88_04280"
     CDS             complement(4258..4968)
                     /gene="deoD"
                     /locus_tag="PLL88_04280"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOJ00812.1"
                     /translation="MSIHIGAKPGQIAPTILLPGDPLRAKFIAETMLKDAVCFNEVRG
                     MLGFTGTYNGKRVSVMGTGMGMPSHSIYVNELIREYQVKNLIRVGTCGGIQLHLKISD
                     VILAITASTDSHMDQLRFSGMTFAAAANFELLAAAYRIAKEMGETVHVGSVLSSDTFY
                     NDHDDWWKLWAENGVLGVEMETCALYSLAAKHGVRALSILTVSDGIAVPQVATTEQRE
                     KGFPLMAKIALRVAEELE"
     gene            5212..5454
                     /locus_tag="PLL88_04285"
     CDS             5212..5454
                     /locus_tag="PLL88_04285"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HOJ00813.1"
                     /translation="MSSLIGVLLVGLIAGFIADKIIKNTFGLWGDLLIGVGGAFLGYW
                     IFGLLGISVGSFFGEIIAAVIGAVILLLVINKFKKS"
     gene            5582..7132
                     /locus_tag="PLL88_04290"
     CDS             5582..7132
                     /locus_tag="PLL88_04290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOJ00814.1"
                     /translation="MVPVRYVLIGINTLSTLLSAYASIASWRRREKAGFAGIYLAISM
                     AATTIYAFGYTMELASDTLQQILFWIKIEHLGVQFIAFPFLFFTLCLSGRQKLIKPQF
                     VVFFSVFALVLIASAWTNQMHLEPHLTPGAPFPTLSYTRGLWTWLSIGNVSFCLVLSI
                     ATFISMSLHAVSTFRKQMLMFILGSLLPLAGMIITATSRVAYSLDLAPLALSVSGILF
                     IIGITRFQIFDIIPLARDMIFENMDNGFVVFDSQERLVDFNLRAQKILPEIGDQSVGI
                     AANRIFCSRSSLLELIQRGSGETINLQKGNAIYRCRMLPLSNPQDKLIGKVVTLENYT
                     EVDAMVKQLKELATMDPLTGIYNRRYFYEVAEKEIARSIRLKKQLSLILFDLDNLKFI
                     NDSLGHSAGDEVLKFVVRLVLQRLRKYDIFGRFGGDEFLILLPETDISAAKGLADELR
                     ASMESSKIPLEDRTVQTTGSFGVADILRDHPMPLEDLLSRADKAVYVAKDGGRNRVCI
                     EKANLAGS"
     gene            7196..8533
                     /locus_tag="PLL88_04295"
     CDS             7196..8533
                     /locus_tag="PLL88_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00815.1"
                     /translation="MRSPRFFPILFYALCLCCQACTGSQVTQETPLVASATFSSISTP
                     TSVEAIEARSFSSTPIPLDEQNPLCQPETVDSMFNSACANGALKIRQNENRRGLDIFL
                     ARMTALKHSTPFTLSATITSRKANSSAMDENQYGFFILDASGMEYALRFQGQYFNMES
                     WDDNEEGKAFERYNMSYFPFLNTNGQANLVQFSCTDEICDLYLNHEFSARFPLDLRGG
                     ISGVGIFAASTWDERFGEVTIQDLTIQPSADAPLLSEPYALEDDLTADHGTFSQSGLS
                     GAFNIFAEDGFHFSSLIPFGYYAAKTNPSLADVAVSTTVHMEIDPTGSSSRYAGLICR
                     SSQDGMYMAVIRADGSYSIFRDTSQRPFALLAERKSDAILSGISENLLKLECIGDQVN
                     FYINGTQVEALTDHRYGLQFGRSGLFTKTGGEPSADAIIFKDFVIEEIRDRKN"
     gene            complement(8530..9432)
                     /locus_tag="PLL88_04300"
     CDS             complement(8530..9432)
                     /locus_tag="PLL88_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00816.1"
                     /translation="MKKRTLVSFCIVFLVSACSIATPNADLSAAITDDVPRSVAATAV
                     SPTETAELTPTQDARRLPEDWQNWSVIPELTATARAIYAQGLQMGNDPHAFSKIGDCQ
                     NLKTVFLWKFDHLDIYEFEWDIAPYLDTVENFQGYFDRDGQSAQYGFTAASPLTQIQA
                     DPEHCQPDETPMECELRTTRPSFVLISLEFPFNGRTAGLYEQYLRRIIEYSMSQGAVP
                     ILATKADNVERDYSINLTVAKLAYEYDLPLWNWWAAAQPLADHGIDPYRDGFHISEQA
                     WEERSKTFLQVLDHLWKQANMVND"
     gene            complement(9926..10324)
                     /locus_tag="PLL88_04305"
     CDS             complement(9926..10324)
                     /locus_tag="PLL88_04305"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HOJ00817.1"
                     /translation="MPGPLLEKWKDRANSLKRETYALYLAVRDPRTPWYAKAFAWLVV
                     AYAFSPIDLIPDFVPLLGYVDDLILLPLGAALAIKMVPAEVMKECRARSEQQFASGKP
                     VNRTAGLVIALIWLAIVFLVVHCAYREFVK"
     gene            complement(10335..11171)
                     /locus_tag="PLL88_04310"
     CDS             complement(10335..11171)
                     /locus_tag="PLL88_04310"
                     /inference="COORDINATES: protein motif:HMM:NF022431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HOJ00818.1"
                     /translation="MRELELKAKLRSSKESNFATTRNEALTLMPAMLEAIGSVDGELR
                     DDLIYAAFVQWIYVQRLVPAQDMRAILALMLDDHHMFYQIGEVPEDGVFTRAFSVLVL
                     PLLLAVHRSTAYLSAVELQQVKVALIRFFDEERDKRGFVQEKGWAHAIAHAADALDDL
                     ALCGEMQATDLQEMLMMTQKVICDPAQVYIHSEEERLGRVVISVISRNMLGIGQVNDW
                     IASFQELVLAEKRVPQSMYLRSNVRNFLCSLYFRLHWKNWLQPYGNALNQTLRAISRF
                     AE"
     gene            11682..12128
                     /locus_tag="PLL88_04315"
     CDS             11682..12128
                     /locus_tag="PLL88_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00819.1"
                     /translation="MNPITAEKDYEGRGYTETVVMEGDKVAWARLDPTDASAVQIAVS
                     RELLGTEEFLWGAWADGGIKDPSLFDYNDHFGVSEAGSPLNTSEDYPLQALASLDNTC
                     RLPYGIDQLSNAITGMCITSQPNQSCVCAYWAVFGATRVCLLWDCD"
     gene            12184..13188
                     /locus_tag="PLL88_04320"
     CDS             12184..13188
                     /locus_tag="PLL88_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00820.1"
                     /translation="MHKKGFHIILFTILMLACACGSKPEADSAKKPTDKPSIVLDATA
                     PTEKEKPPTDLMPGEPNPQAERTLEDADASLRAYERRVVSGDNILDNLYERPFTSQVM
                     DYQPDLNILTAAIVSDDAFIYFTITLASVDPVSGILNGTYGIEFDRTQSGRGDLLVLV
                     SQPGTEWSMKELVAYTDTTGSVGGARPIVAEAGYPVDGYTEIVELGGDKVAWARMAPE
                     DTYAVQIAVSRALLGTEEFLWGAWADGEVKDPSLFDYNDHFAPVVAGSPIKTDTDYPL
                     KELASLDNTCRLPFKLENVRGIPGMCTSIPAASSGSSCVCVRWSSPLIALVCLEWDCD
                     "
     gene            13334..14245
                     /locus_tag="PLL88_04325"
     CDS             13334..14245
                     /locus_tag="PLL88_04325"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOJ00821.1"
                     /translation="MKTFGRILLMSLCLPIILVGIIWFVTRQANDSLESDGAKRRYML
                     YVPKSYDPATPASLVISLHGFSDWPAHQMHMSGWNELADEEGFIVVYPLGTGFPLRWQ
                     LYDFDHPSANPTADIRFIAELIEHLEQKYNIDPSRVYANGFSNGGGMAHALSCALSGQ
                     IAAFGGVSGAYFYPLTACRTTRAVPAILFHGTADAVVPYAGGPTQRFPYDFVNLPDFA
                     QELATRNGCTATPRTEQISQKVHSITYTACVNNADVVFYSIAGGGHSWAGGRAMPRII
                     AGETNMEANATRLMWDFFQNHPLVEDH"
     gene            14320..14445
                     /locus_tag="PLL88_04330"
     CDS             14320..14445
                     /locus_tag="PLL88_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00822.1"
                     /translation="MNSALLLLTVGLILVFAPLVSAEEKQARAGNAWKGKWLERG"
     gene            complement(14499..15098)
                     /locus_tag="PLL88_04335"
     CDS             complement(14499..15098)
                     /locus_tag="PLL88_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00823.1"
                     /translation="MNDFISMTCPSCGGQLQVRKDLQKYFCMYCGSELVLKQDSDGVF
                     STIQARDLQASAKLKEIQFSMAAMELLKSQVAEIEKEIRKIRRTFLEYYPTIYSEALN
                     KKYFSDYEKEKNLPVQLSRLCVSYWDDWMNYIEKDIPGYSSAEELIEFAQFVQRSAYK
                     RNKYVTTILTILEPLPSLAGQLKQKKQQLSAMLEQAINQ"
     gene            complement(15121..15708)
                     /locus_tag="PLL88_04340"
     CDS             complement(15121..15708)
                     /locus_tag="PLL88_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00824.1"
                     /translation="MNDFIALTCPSCGGQLQVQNNLQKCHCTHCGTQLLLKQDDSGML
                     IPLQARDLQASAKLKEMQFSEKAMDLLKGQIAELEDRFAKIRNAFLTDVIALRTARCF
                     KDYEKENFITPGINKFCVLNWDNWFNPAWDIMGYTSVDDFLTLYAFMQQPKYQKDKVL
                     LPLQKLFEPLPTLAGELKAKKARLNAMLEQAVQNQ"
     gene            complement(15837..17300)
                     /locus_tag="PLL88_04345"
     CDS             complement(15837..17300)
                     /locus_tag="PLL88_04345"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HOJ00825.1"
                     /translation="MCSVFHEFKEKYPELKKFEERIHQLVADGFFASRQDIFINRTPG
                     RLDLMGGNDDYTGGLVFEKTIREATMVAVQPRTDMLFRFYNPAVKNLDWEDFISFSLT
                     DLMEGEHVKPMEKVRDWINADPHRSWCAYLIGDLYYLLEHFPAKVQHGFNLYLESDVP
                     LGKGVSSSAALEVAGMKGMAACYEIKVSGVELATWTQWVEIALTQSACGIMDQLAVVM
                     GDEGYFVPMLCQPCQPYQLVRLPENLHVWGVDSDVRHAVSGIEYESARAATFMGYRYL
                     CEWEDLQPTLDQSGALPRWVETKWNGYLANLSPSLFSQLYEERLPASEEGSHFTQQFA
                     IHLDPFTSVRNEIEYPVRGATRYAVEENWRVHAFCSLISKPVDLVDETTCRLLGELMY
                     QSHQGYTDCGLGSEYTDTIVELVRAEKEQGLFGAKITGGGAGGTVAVLGEQSPRAQAA
                     FQRVVDRYQTLRKKDPYVFEGSSAGCDVFGVQRFVLA"
     gene            17804..18577
                     /locus_tag="PLL88_04350"
     CDS             17804..18577
                     /locus_tag="PLL88_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOJ00826.1"
                     /translation="MNQKKSTHKLEAIIVGGGTAIPVLDHSPPSLYLKFKNLSALLDV
                     GPGTFSKLPIYGINPLLINNIFVSHLHPDHVLDLVIYYMIRENAAKKKNSPVNVIGCK
                     GIKNFILQLNRIFPDISFSNPAIRTHEMANDEYILGDIRVRTILTGHTPHSIAFRFDF
                     NNISFVYTSDCIYSQQLVEFCAKADILVGECSFSDSYPTSDHMNAQKIGSLAENAQVQ
                     EMFITHCYPQALKKDLKKQISKYFSGPIHITQDGDRINR"
     gene            18582..19454
                     /locus_tag="PLL88_04355"
     CDS             18582..19454
                     /locus_tag="PLL88_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ00827.1"
                     /translation="MKYATQLLNKSSIKTRGLNWFALIPLLIFSIGFQIIPIISLISS
                     SFSTKEGLSIENYQRAFTPHIAEAFRNSLKLSFTTAFLGVGLGLVVAYAIISTEKKFM
                     KNALIALADVTTNFGGAPLAFAFIVTLGSTGVITLILKSMGVFLYPSFRIYSISGLAI
                     AYLYFQLPLMIMLILPALQGLKKEWWEAAINMGANSMQFWARIGLPVLTPSLLSSFFI
                     LFANSFGAYATAWTLTGPDVNLITVQIASLIRGEVQLEIGLADALAVISLFIILFCVG
                     GYLLLSTKAKRFRT"
     gene            19451..20263
                     /locus_tag="PLL88_04360"
     CDS             19451..20263
                     /locus_tag="PLL88_04360"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00828.1"
                     /translation="MRMNKLLRSIVLAVFLLFMIVPLIATLLFSMSTRWDRTILPEGY
                     TLEWWKTVISRGAFGLTLKNSFYISFATATALSILVTPTAYWVHIRMPKNKIIFEIMS
                     AFSFGIPGVILALALIRFYSGVPLPLINTTNILLLACMELCFPFMYRPVANSLDSIDI
                     HTLTEASQSLGGNWFATFIHIILPNILPGIASGFLLVFSTVFAEFTLTNLLVGARFKT
                     FPIYLVEFTRFDGRQASALAIISFVVAWVVSTLLLLVSGKRALSPDNLTIGH"
     gene            20340..21461
                     /locus_tag="PLL88_04365"
     CDS             20340..21461
                     /locus_tag="PLL88_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ00829.1"
                     /translation="MSKKTITMFLLLTVLITLGACQTSVEEEVAEGPAAEEPITEEPQ
                     VSEKELMIEAANEEGELISYGMSDDWVNLGNIWKAIETNYGVVHMDTDMTSMEQITHL
                     LAEMEAPVMDVADIGFDSLGLLLENDLAMEYKSEYWDKFDEDFRDPDGKWAVGYWGAI
                     AFLVNTDLVENIPQTWADLLKPEYHDQICNRDPRVSTYATGAVLAAAYANGGGEDNVQ
                     PGLDFFEQLRDSGNWREGVVLNVASVQKGECPISIVYDFDGFNKRDATGLPLEVVIPA
                     DGTVGMLFAQYINKLAPHPNAAKLAIDFFYSDEGQIMFAEGYAHPGRSDVVLPDDVIA
                     KLLPESAYGELHFPSNLENFSAAMQQIKDGWEAIVGESE"
     gene            21763..22575
                     /locus_tag="PLL88_04370"
     CDS             21763..22575
                     /locus_tag="PLL88_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ00830.1"
                     /translation="MPFLAVCLTFEFVPIFFLIRGSFLDKATQSYTLAHYQALARTVY
                     VNSFINSIKISSLTAIIGVVLGTLIGYAIFRLPKEGFKNALISLSDVTTNFAGAPLAF
                     AFIIILGINGVITQFLLKYFNYELYPAFSIYSFSGLVLAYTYFQLPLMVLTIIPAFVG
                     IKKEWQESAESLGANKIQFWWHIGIPVLTPSLLAGLTLLFANAYGAYATAYTLVESQL
                     SLVTLQIGFMIAGEVRHDPGVGMAMSVLSLIIMGLSIVVYQIATTRVRRWAK"
     gene            22593..23435
                     /locus_tag="PLL88_04375"
     CDS             22593..23435
                     /locus_tag="PLL88_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ00831.1"
                     /translation="MTNRRTSTFKKARTHLQLWQVVLIALFIFYMLLPIFSTYIFSIA
                     TRWDRTILPEGITSEFYQEATKASYFSSSLRNSLILSLSTILVSLVIIIPTVYWVNVR
                     LPQAKSILDILMILPFGIPTVVLALALVQVYNGTPLGRTPQLLIGAVFIYSMPFMYRP
                     VSNAMRSIDALTLTEAGQSLGASTLQVMIQVIIPNIFSGIISGSLLVFATVFAEFTLT
                     KLILGARFKTFPLLLVEYTRINGNIAAAFSVISFTIAWLISLLILWIGKKGQGSSTQA
                     PTAH"
     gene            23451..24515
                     /locus_tag="PLL88_04380"
     CDS             23451..24515
                     /locus_tag="PLL88_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00832.1"
                     /translation="MAYLELRNIHKSFGNVTAVNNFNLDIEKGKLVSFLGPSGCGKTT
                     TLRMIAGFEQPDEGTIRLGNQDITVISPNQRDIGMVFQAYALFPNMNVRQNIAFGLKM
                     KKTPKAEIEKRVDEVLEMVRLKDTAKRFPHQLSGGQQQRIALARALAVRPRVLLLDEP
                     LSALDAEVRVALRGEIRRIQSDLAITTVYVTHDQEEALSISDQVVVMNNGIIQQVGTP
                     EEIYRSPATRFVATFIGTANQFFGNVAGECKVQSNGLTITTRKLSDFSSGQKVVVLVR
                     PELIIVSNAQPDTNSGNTIPGMVETITFHGAVTRLGVHVNQQRVVADITMTSSGYISL
                     NQKVWLSFKPDACKVMAVDE"
     gene            24512..25978
                     /locus_tag="PLL88_04385"
     CDS             24512..25978
                     /locus_tag="PLL88_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00833.1"
                     /translation="MIYINGHEIVLDKQEQLEAWTSYGQIVKLAMDFIQSCPRDPRNN
                     LPWYLQYSCFWTDPLRPADWPDNPAGKFAWAVTAILKYYPYSGKKDFVDIAVDMLDHL
                     WDQRTPDSFLWNEVPYASAHPGTGIYFGARADGEYVTEPDKIAQVGKAYLDLYKLTVE
                     ERFLQRAEHCAQILSNNLRPGDQDHSPLPFRVNVQTGNIVEEYTAHMITVVRLFDDLI
                     NLGKGNYQKKRESLLGWIQRYPLQNNKWKGYFEDIRVDPGNENRDQLSPIETARYILE
                     HRSQFPHWQTMVQGLIEWVRETLGSQPFFSAIPIHEQKFCYFVMGSHTARFASVCAQF
                     ADLTANVKYHEMAVRSFNWASYMANVDGTVTVGIDRPDYYNQCWFTDGYFDYVPHFLD
                     GMASLPDLAPDDEDHLLRSTSIIQDISYQTNEISYQSYDANGCQRFRLTFLPKEVLAD
                     DKPLQKKAEPDEKPGWSVGMVPYVVDIYSGARNVTIRG"
     gene            26082..>26670
                     /locus_tag="PLL88_04390"
     CDS             26082..>26670
                     /locus_tag="PLL88_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ00834.1"
                     /translation="MSKKFLRLIVFLVLFSIIAAACSTTSAGEGAAVEEPAVGEAAEL
                     DPMEQLIIDAKNEGVLVSYGLPDSWVNYDEMKTLLLEKYSIETQDTDMGSGEIIAALG
                     AEAGAPVADITDLGLNFAKIVEDEELSQPYFHSYWDELPDYAKDPEGRWSSAFWGAIA
                     ILVNKDAVTTVPQTWSDLLNDEYIGKVCIKDPRSSA"
BASE COUNT         7322 a         6365 c         5961 g         7022 t
ORIGIN      
        1 cacatccttt ccgaagatga gatcgcttat atagcgcttc acattggcgt tcttatggaa
       61 gagaagaaag cctattcaga aaagctgaaa tgtatcgttc tagcacctga ttactatacc
      121 gtgggtcgct cattgtaccg gagattgaat gaaaaattct ccgacagcat tttgatcacg
      181 aacttcatca cttcttatga cgatctcgcc agtacagaag aatatgacct gctcttatcc
      241 accatcgaaa taaatccccg cacagaaaaa ccccacatat tgatggaccc atttctctca
      301 gataccacca tcagcaacgt ttttcagaaa atcgaggagg taaaacaggc aaaaagaaaa
      361 caaaagatga caaatcaatt caagtatttc ttccggaatg atttgttctt ttatgactac
      421 ccattcaaaa cgtaccagga tgccatcgag accatgtgcg attcgatgat ggaaaaagat
      481 tatgttgata agaactataa gaaagaaatc tatgagcacg aggaagttgc accgagtgcc
      541 tatgggaata tcgccattcc ccacccgctc tccaatcgag ctatttcttc cgtgatcgca
      601 atttctatca atccaacccc cattcaatgg ggtacgaata aagtgcatat tgtttttatg
      661 ctctcgctcc aagaagagaa ccgcgatttg tttacggata ttttcaactc cataacccac
      721 ctgatatcaa acaaagactt ttttaccaag atcatgaatg caaagacgta tgaagatttc
      781 attgaaatct tggtatcctc cactgtcaat taagcacagg ctgcatcgcg attgtttttt
      841 taatccccat tcagtcatgc cccatggtcg ttgttgaaac aacttcatga tttttgcaga
      901 gtatctctgc aaatgtcaaa attgtttaag gtctctattt tcactatcat acaaacagag
      961 cccgccgtga aatcaccatt ctccaacccg gttggtctct taacagattg ctatcttcaa
     1021 atagcaacct gcccaaatcg gcgtttcttt gctaacgaaa aggcaatttt cgaaagaact
     1081 ttcttataaa aaccatgctc gactcgacga cagacattcc aggaggatcc attgcatgaa
     1141 atattcccac ctatttgaac cattaaaggt caataccctg atcttgaaga atcgtgtcct
     1201 cttggcacct atgggatacg ctgaccctgt aacgctttcg gaacaggcgt tgggaggacc
     1261 ttccttgatc accatcggca gtgcgcaaat cgatgaagag agttcgaaca tcatgaacca
     1321 accctaccca ttttcgaagg gcggattgac cagactccgt gaacaggttg tgaccatacg
     1381 ccaggcggga gcgaaagcat ctttggaatt gatccatggc ggtcagttcg ccgggaagag
     1441 ctacatcagc agcaactacg ccattggacc catggcatta attcgcgagg atggacttga
     1501 agtaaaagcc atggacgagc cgatgatgta tgagattgca gagcgttatg cgaatgcagc
     1561 caaagaagcg caagcggtcg gttttgacat gatcatgctt cattttgcac acggctggtt
     1621 gccagcccaa tttctatcac cgcattttaa taaacggacc gatcaatatg gcggaagtct
     1681 tgaaaatcgt gcccggtttc ctaaaatgat catcgaacgg gtgcgtagcg caatagggaa
     1741 ggattttgct gttgaattaa ggatcagcgg atacgaatgg atagatgaat ccattgaaat
     1801 cggggatgtg atcagttttg taaaatccgt ggaaaacctc attgacatgg tccacgtttc
     1861 agccggaatg gatggggcgg ggattgatga aatgttaagc gctcattgcc atatgatgac
     1921 caccaccttt gaaccgcaca tggctaacgt atggttggct gaacgcgtga agaaggctgt
     1981 caatattcca gttgtaacgg ttggcgccat catgcatccc gacgaagcag aagatattct
     2041 ctcaaccggg aaagcagacg cggttgccat tgcgcgcgca gttgtcgccg atcctttatg
     2101 gtctcaaaaa gccctggaag gaaaggggga ggagatcaca ccctgtttgc ggtgcctttc
     2161 ttgttcacac gttgcaacca gaagaaggaa cagttggggt tgtgcggtga atccacgcca
     2221 tggaagggaa gcgttcgttt ctgccgttat cgagaaagca cccatgataa agaatgtcgt
     2281 tgtgatcgga ggcggacctg gcggaatgaa ggcagctatt accgcacgac agcgcggaca
     2341 caccgtaacc ctcctggaaa aatctggcga gttgggtggc aatataaaat tttcagaata
     2401 tgacaaattg aaacttgact taaaactttt taaagattat ttgatccgca ccgtcaagca
     2461 aagtggcgct gccatcaagc ttaacacaga agccacacct gaaatggtgc gggaaatgca
     2521 tcccgatgtg atcatcgtgg cggtcggcgc aaatccgatc acgcccaata tcaagggcat
     2581 taagattgag aagaaaaacg tcatgcagtc aattgaagcc tatccaaact tggagaagat
     2641 cggcaagaga gtcgtggtta ttgggggcgg ctacattggt tgtgagatca gcctggaatt
     2701 ggctctgcgc gagcatgaag taaccatcat tgaaatcagc gacgtcctcg cttctaatgg
     2761 aaatcgattg tacaaagaag ctcttcatca aaaaatggca acctgtacag gtttgcaata
     2821 tcatcttgaa acagtttgca ccgaaattca ggataacagg gtagtcatac aaggcaaaga
     2881 cggcgttgaa aaaagggtag agaccgacac cgtaatcctg gctaccggct tcaaggccaa
     2941 tcgaactgta gctgaagcct tctacggcat cacatacgaa acatatctga tcggtgattg
     3001 cgaacgggca cgaaaagtag attatgcgat caacgaggga tattttattg gcagaaatat
     3061 ataatacaaa aaagaccctc aggctatgaa aataccgtga gggtcttttt ttatcatgca
     3121 aacagccagg ggttatctta caaagaccat ccaatagaaa agactcaaca atacttgggt
     3181 gacaacccct gccaacaaac ccaacaggaa ttggttaacc ctatccatga acgggaaagc
     3241 cttgacgatc acgaagcgaa tccacaacca aatccacaaa gtcaataaag caacggtgat
     3301 gaacattttg gttctttctg cgattgaaca aacttgattt tccgtaaaag ggcacagcgc
     3361 attggcacag cagacaatgg atattgttca atcggaccat tttcatgacc aacatccttg
     3421 ccagagaggt tcactttttt aaagtggctg gaaaacctct tactgtgtca atcgtccata
     3481 cagatgcggt tggcgatgct ctatgaacat ttcagaacat tgtgtgaggt ccaattcaac
     3541 ggtcaccaaa cgattcgcat cccgggtctg caccaggcag cttccatcag gtgcgtacgc
     3601 atacgctaat ggaatggcat cgttgtagtg actgcaccca accgcaaaaa cgccattctc
     3661 tgacgcacgg cagagtccag ccgccgtcca ttgcgcaaat tgcgtttcat gccacatgcc
     3721 ggttccgata gggttgaaca ggatcctcgc tccttgcaga gcgtaagagc gcgccacctc
     3781 cggaaagtgc acttcataac aaatggcaat tcccaccaac ccgaagtccg tagcgatcac
     3841 atcaagatcg tctccgtggc tgaacccttt ggaaagctca ttgtacgtaa gggatgtttt
     3901 gcggtgatgc ccaaccacct ccccagacgg attcacgacc actgcatgct taaatttttc
     3961 tttgcctgcg tcgcggtctt ccatcccaca gacgatccat ttctttctgc tcctggctat
     4021 ctcctgcagc gcgctcagat tttcagaata gatatggtct tcaggaaaaa gaaaaatatc
     4081 cggtttggca tcagcggtat attgctctaa ttccaccagg ggtgtcgcgc tatctttgac
     4141 tggtcgggga atgaccaacc caatatgaat gctattttgt gccatatgca tctccaccaa
     4201 taggtttttt cttcaatcaa taaaattttt tcgtacgcaa gagccgaaga gaaattttta
     4261 ttccaattct tcggctacgc gcaaggcgat ttttgccatc aacggaaaac ccttctcgcg
     4321 ctgttcggtg gtggcaacct ggggaaccgc gatgccatca ctgacggtga gaatggataa
     4381 tgccctgacg ccatgcttcg cagccagcga atacaatgcg caggtttcca tttcaactcc
     4441 cagcacccca ttctcagccc atagtttcca ccagtcatcg tggtcattgt agaaagtatc
     4501 gctggagagc acgctgccaa cgtgcaccgt ttctcccatc tctttcgcga tgcggtacgc
     4561 cgctgcaagc agttcaaaat tggctgccgc cgcaaaggtc attcccgaaa aacgcaactg
     4621 gtccatatga gaatcagtgg acgcagtaat agccaggatt acatcgctga tctttaaatg
     4681 aagttgtata ccgccgcagg tcccgaccct gatcaggttt ttcacctggt actcacggat
     4741 cagttcattc acatagatgg aatgggaggg catgcccatg ccggttccca tcacagaaac
     4801 tcgttttcca ttgtacgttc cggtgaagcc cagcattccg cgcacttcgt taaagcaaac
     4861 cgcatctttg agcatggttt ctgcaatgaa ttttgcccgc agcggatcgc cggggagtaa
     4921 aatggtgggg gcaatctgcc cgggttttgc gccaatgtga atactcatgg atcactcctc
     4981 tgtctctaaa agtccgttta ttatatattt acttctaaag aaatcccatc ctaaaaccaa
     5041 ttctgaccat ctttcgtaaa aggtatggca acaacgcctc cgctcagcaa tggcatgaat
     5101 ctgtaagacg aaagcgaacg atgataaacc cgcaaaattt ttatgtttct tttatttttt
     5161 acaaagaatt tggatataca attaatctaa agttcaaaaa aggagagtgt gatgtcaagt
     5221 ttaatcggag ttttactcgt tggtttgatc gcaggtttta ttgctgataa gatcatcaag
     5281 aatacctttg gattatgggg agacctgctc atcggtgtag gtggagcatt cttgggttac
     5341 tggatctttg gtctgctcgg gatctccgta ggttcattct tcggtgagat tatcgccgcg
     5401 gtcattggcg cagtgatttt gctgctggtc atcaacaaat tcaagaagag ttagcgatat
     5461 tgccacacaa aagcagaaaa gatggagaga aagactctcc atctttttca tattgttccc
     5521 atcagtcatc attatcggtt tgtccttatg ataaggtcat tttatggttc gatttatgga
     5581 tatggttcct gtacgatacg tacttattgg tataaatact ctttccaccc tattatcagc
     5641 atatgccagc atcgcatcct ggagaaggcg agaaaaagcg gggtttgcag ggatatatct
     5701 ggcgatcagc atggcagcca caaccatata cgcttttggt tacacgatgg agctggcaag
     5761 cgatactctg caacaaatcc ttttttggat aaaaattgag cacctgggcg ttcagttcat
     5821 cgcatttccc ttcttgtttt ttaccctctg cctaagcgga cggcaaaaat taatcaaacc
     5881 ccaatttgtc gtgttctttt ctgttttcgc tctggttctc attgcatcag cctggaccaa
     5941 ccaaatgcac ctggaacccc acctgacccc gggagcacca ttccccaccc tttcgtacac
     6001 gcgcggatta tggacgtggt tgagcatcgg gaatgtgtca ttctgtctcg tattgagtat
     6061 cgccaccttc atcagcatgt ctttgcatgc cgtatccaca ttccgcaaac aaatgctcat
     6121 gttcatcctg ggttctctgc tccccttggc aggaatgatc attacagcca ccagccgggt
     6181 tgcatatagt ctcgacctgg cacctttagc cttgagcgtc agcggtattc tctttatcat
     6241 cggtataacc cgctttcaaa tattcgatat catccctctg gcacgcgata tgattttcga
     6301 aaacatggat aatggctttg tcgtctttga cagccaggaa aggctggtgg atttcaatct
     6361 acgagcccaa aagattcttc ctgagatcgg agatcagtcc gtaggcatcg cagccaatag
     6421 aattttctgt tctcgttctt cattgttaga actaattcaa aggggcagtg gagaaaccat
     6481 caatctgcaa aaaggaaacg cgatataccg ttgcaggatg ttgcctttat ccaatcctca
     6541 agacaaatta atcggcaaag tggttactct ggaaaattac accgaagttg atgcgatggt
     6601 caagcagcta aaagaactgg ctaccatgga cccattgacc ggcatttata accggaggta
     6661 tttttatgaa gtggcagaaa aggaaatcgc gcgttcgatt cgattgaaga aacagctttc
     6721 gctcattctt tttgatctgg ataatttaaa attcatcaat gactcgctgg gacattccgc
     6781 cggagacgaa gtgctcaaat ttgtggtacg gctggtttta cagaggttga ggaagtatga
     6841 catttttggt cggtttgggg gtgacgaatt cctgatcctg ctccccgaaa ccgacatatc
     6901 cgctgccaaa gggttagcgg atgaattaag ggcatcgatg gagagttcaa aaatccctct
     6961 ggaagatcga actgtgcaaa ccaccggcag ttttggcgtg gcagatatcc tgcgcgatca
     7021 tcctatgcct ctcgaagacc tgctaagtcg cgccgacaag gcggtttatg tggctaaaga
     7081 cggcgggcgc aatcgcgtct gcatcgaaaa ggcaaatctt gccggttcat aaaagcaaac
     7141 tcaatttcac agtcgcatat ccttatgcat aaccaaggtt gtttataatg atgtcatgag
     7201 atcgcccagg ttcttcccaa tactcttcta cgccctctgt ttgtgctgcc aggcatgcac
     7261 tgggagtcag gtcacccagg aaacacctct ggtagcgtct gcaacgtttt cctctatctc
     7321 cacccccaca agcgtggaag caattgaggc acgctccttt tcatcaactc ctattccact
     7381 ggatgaacag aaccccctct gccaaccaga aacggtcgat tccatgttca actcagcatg
     7441 tgcgaacgga gccttaaaaa tccggcaaaa cgaaaacagg cgtgggcttg atatctttct
     7501 tgcgagaatg acagcgctga agcatagcac accgttcacg ctctccgcga caatcacctc
     7561 tcgaaaagcc aatagctcag caatggatga aaaccaatac gggtttttca tcctggacgc
     7621 atctgggatg gaatacgctc tccgttttca aggacaatat ttcaacatgg aaagttggga
     7681 tgacaacgag gaaggaaaag cgttcgaacg atataacatg agctatttcc ccttcctgaa
     7741 cactaacggt caggcgaacc tagtccagtt tagctgtacg gatgagatat gcgaccttta
     7801 tttaaatcat gagttcagcg cgagattccc gctggatctt cgcggtggga taagtggagt
     7861 gggcatcttc gcggcttcaa cctgggatga acggtttgga gaggtcacta ttcaagacct
     7921 caccatccaa cccagtgcag acgctcccct gctttcagaa ccctatgcgc ttgaagatga
     7981 tctcactgcc gatcatggca ctttctccca atctgggctg agcggagctt tcaacatctt
     8041 tgcagaagat ggttttcatt tctcatcgct catccctttc ggttattacg cggcgaaaac
     8101 aaacccctcc ctcgcagatg tggcagtgag caccacggtg cacatggaaa tcgatccaac
     8161 gggcagctcc agccgttacg ccgggctgat atgccgttca agccaggatg gcatgtacat
     8221 ggcggtcatc cgcgccgatg gcagctactc gatctttcgc gatacctccc aacgtccttt
     8281 cgcattactg gcagaaagga agtcagatgc gattttgagc ggaatcagtg agaacttgct
     8341 aaaactggaa tgcattggag atcaggtcaa tttttacatc aatggaacac aggtggaagc
     8401 gctgaccgac caccgctacg ggctgcagtt cggacgcagc ggcttgttca ccaaaacagg
     8461 tggagaaccc tctgcggatg caatcatttt caaagacttt gtgatcgaag aaatccgcga
     8521 tcgaaagaac taatcgttta ccatattggc ttgcttccaa aggtggtcaa gcacctgcaa
     8581 gaaggttttg ctgcgttctt cccaggcttg ttcgctgata tgaaaaccgt cgcgataggg
     8641 atcaataccg tggtccgcca gcggctgggc agccgcccac caattccaca aaggcagatc
     8701 atattcataa gccaactttg ccaccgttag attgatggaa taatctctct ccacgttatc
     8761 tgccttggtc gccaggatgg gcaccgctcc ctgcgacatg ctgtactcaa taatacggcg
     8821 caggtactgt tcatataacc ccgctgtgcg cccattgaac ggaaattcca acgagatcag
     8881 cacaaagctt ggacgcgtgg tgcgcagttc gcattccata ggggtttcat cgggctggca
     8941 gtgctcggga tcggcttgaa tttgggtgag cggcgaagcg gcagtaaagc cgtattgggc
     9001 tgattgccca tcacgatcaa aataaccctg aaagttctcg accgtgtcca gatacggggc
     9061 aatatcccac tcaaattcgt agatatccag gtgatcaaac ttccagagaa aaaccgtctt
     9121 gaggttctgg cagtcgccta tctttgaaaa ggcatgcgga tcattgccca tttgcaaacc
     9181 ctgtgcatag atggcgcgtg cggttgcggt caattcgggg atgactgacc aattctgcca
     9241 gtcttcaggc aaccggcgtg cgtcttgcgt gggtgtaagc tctgcggttt cagtagggga
     9301 aacggctgta gcagcaacgg atctcggaac atcatctgtg attgctgctg aaagatcggc
     9361 attgggggtt gcaattgaac aagcgctcac aagaaaaaca atgcagaaac tgaccagggt
     9421 tctttttttc atacggatca ttcacgtatt ttcagaattg accacaccgt caatcttgag
     9481 gtaggtttgc aatggattgg aatttttcca atttcgttcc gtcattgtta ttccttcggc
     9541 gaaatctgaa atttctcaga gtgattcctg cgcataatta tatatctatc gacaggtcat
     9601 tccaaccaat agaaacattt tcaataacaa ggttcatacc agtggtccag tgatgggctg
     9661 tactcagatc attcaggcac aggttcataa acggaatggc acagaagaaa gattccgaat
     9721 ccactcgtga tccatccatc gatcaatttc gtgggaactg cccggcgatt tgctttacct
     9781 tggcgcgtgc ggtctctata gctgcatgtc tcggggcatg tactgggtgg ttctttctat
     9841 gaccaccttg cttttggaca cgctatacaa cctgaacgca tcagcggtcc atgcctgcag
     9901 tattgcgctc agcccaaatc aggagctact ttacaaattc cctgtacgca cagtgcacca
     9961 ccagaaacac gatcgccagc cagatcaaag cgatcaccag accggcggtt cgattgaccg
    10021 gttttcccga tgcaaattgc tgctcgctgc gggcgcggca ttccttcatc acctccgccg
    10081 gcaccatttt gatcgccaga gcggctccca acgggagcag aataaggtca tccacatacc
    10141 ccagcagcgg cacgaaatcg ggaatgagat cgatcgggct gaaggcatac gccactacca
    10201 accaggcaaa tgctttggca taccagggag tgcgcggatc gcgcaccgcc agatagagcg
    10261 cgtaggtttc acgtttcagg ctgttggcgc ggtctttcca tttttcgagc aatggtcctg
    10321 gcatgccgag aattctactc cgcaaagcgg ctgatcgcgc gcagggtttg gttcaaagca
    10381 ttgccgtagg gctgcaacca gtttttccag tgcaggcgaa aatagaggga gcacagaaaa
    10441 ttccgcacat tgctgcgcag gtacatgctt tgtggaaccc tcttttctgc cagaaccaac
    10501 tcttgaaacg aggcaatcca atcattgacc tgtcctattc ccagcatatt gcgtgaaatc
    10561 acagagatca ctaccctgcc cagacgttct tcttcactgt ggatatacac ctgcgcggga
    10621 tcgcagataa ccttctgtgt catcattagc atctcttgca ggtccgttgc ctgcatctcg
    10681 ccgcatagcg ccagatcatc cagcgcatct gccgcatgcg cgatagcatg cgcccaaccc
    10741 ttctcttgca cgaaccctcg tttatccctc tcttcgtcga agaagcgtat aagggctact
    10801 ttgacttgct gcagctctac cgcactaaga taggcagtag agcggtgcac tgccagcagc
    10861 aggggcaaaa ccagcactga aaaagcccgc gtgaaaaccc catcttccgg cacttctccg
    10921 atctggtaga acatatggtg gtcatccagc atcagcgcga ggatcgctcg catgtcttgc
    10981 gcaggcacca agcgctgcac atagatccac tgcacaaaag cggcataaat cagatcatcg
    11041 cgaagttctc catctacaga gccaatggct tccagcatgg caggcatgag agtcagcgct
    11101 tcattgcggg tagtagcaaa attgctctct ttcgacgaac gcagttttgc tttcaattcc
    11161 agttctcgca tttcatcctc ttctttgaca tagatgatct ttcacgaaaa gcatatcatg
    11221 gatcctgaga tcgtttggat tacacattaa aacagtcttg atcgaatgaa ttcgctgatc
    11281 agcccttttg gagcgacgca aggaagcata ggttgttttt tatggctgaa atgctacaat
    11341 tttcataatc aaaaacaaga gcagcaaggg gggacaagct ggctccgcat cacactttca
    11401 tgtttcagac aagcgaacaa aaagcaccac tgccgcccat gttgtacaaa aagaaatgga
    11461 ggttctatgc gcaaggtttt tgtcctactg ttcgtaatta ttcttgttct tacatccgcc
    11521 tgtggagaga agaaagagct tgcgactgaa attgttccca ccgacactac cgcacccaga
    11581 ccgggcgggg agacctgctg gtatgggcaa catactttgc acctgaatgg tctaccgatg
    11641 gcatcaccgt gtatatcaac ccaagcggca cggtaggagg attgaatcct ataactgccg
    11701 aaaaggatta tgaaggcagg gggtacacag aaaccgttgt tatggaaggc gataaggtcg
    11761 cctgggccag gctcgatccc acagatgcca gcgccgtgca gatcgcggtc agtcgggaat
    11821 tgctcggcac agaggagttc ttgtggggcg cctgggcgga tggtgggatc aaagacccct
    11881 ccctgttcga ttacaatgat cattttggcg tatccgaagc cggctctccg ctcaacacca
    11941 gcgaagatta cccactgcag gcgctcgcca gtctggataa cacctgccgc ctaccgtatg
    12001 gcattgacca gcttagcaac gcgatcaccg gcatgtgcat tacctcacaa ccaaatcaga
    12061 gctgtgtttg cgcatattgg gctgtttttg gggcaaccag agtttgcttg ctgtgggatt
    12121 gcgattagag atttcataaa tcaagactgt gaatgaataa tttatatata gaaagaagga
    12181 agtatgcata agaaaggatt tcatataatt ttatttacga tcctgatgct agcgtgcgct
    12241 tgtgggtcga agcctgaagc cgacagcgcg aagaaaccaa cggacaaacc cagcatcgtg
    12301 ctggatgcca ctgccccgac agaaaaagaa aagccgccaa cggacctgat gcccggcgag
    12361 cctaatccac aagccgaacg cactctggaa gacgcggacg ccagcttgcg agcttatgaa
    12421 cgccgcgtcg tttccggcga caacatcctc gataatcttt acgagcgccc cttcacctcg
    12481 caggtgatgg attaccaacc tgacctgaac atcctgacgg ctgctatcgt cagcgatgat
    12541 gccttcattt atttcaccat cacgctggct agcgtcgatc cggttagcgg tatcttgaac
    12601 ggcacctacg gcatcgaatt cgaccgcaca cagagcgggc gcggcgatct gctggtgctg
    12661 gtaagccaac ctggcacgga atggtcaatg aaagaactgg tcgcctacac tgacacaacc
    12721 ggcagcgtgg gcggggcgag gcccattgtg gcagaagcag gttacccagt agatggctac
    12781 actgaaatcg tagaactggg cggagacaaa gtagcctggg cgcgcatggc tcccgaggat
    12841 acatacgcgg tacagatcgc ggtcagtcgg gcattgctcg gcacagagga gttcttatgg
    12901 ggcgcctggg cggatggtga ggtcaaagat ccctcattgt tcgattataa cgatcatttc
    12961 gcacctgtcg tagctggttc tcccatcaag acggatacgg attacccgct caaagagctc
    13021 gccagcctgg ataacacctg ccgtcttccc tttaagttag agaacgtaag gggaatcccg
    13081 ggtatgtgca cctccattcc ggcagccagc agtggatcat catgtgtttg tgtacggtgg
    13141 tcctctccat taatagctct agtgtgtttg gaatgggatt gcgattaggt aaaacgattc
    13201 aataatattg tgggttacga agagacaggt gaatgataag gatcacctgt ctctttcaat
    13261 atatatttca ttattcatta caattagcgg gtcagtcttt gtgtaccttg gttaacggaa
    13321 gaggataaag atcatgaaaa cattcggacg cattttgctg atgtcgcttt gtttaccaat
    13381 tatcctggta gggatcatct ggtttgtaac ccgccaagcc aacgacagcc tggaatcaga
    13441 cggagccaag cgcaggtaca tgctctatgt gcccaaatcc tatgatcccg ccacccccgc
    13501 ctcgctggta atcagcctgc acggtttcag cgattggcct gcccaccaga tgcacatgag
    13561 cgggtggaac gaactggcag atgaggaggg gttcatcgtg gtttacccgc tgggcaccgg
    13621 tttcccgctg cgctggcagt tgtatgattt cgaccatcct tccgccaacc caaccgccga
    13681 tatccgcttc atcgcggagc tgatcgagca cctggagcag aaatacaaca tcgatcccag
    13741 ccgcgtctat gccaacggat tttccaacgg gggtggcatg gcgcacgcct tatcctgtgc
    13801 gctctccgga cagatcgctg ccttcggcgg tgtgtcaggc gcctatttct acccgctgac
    13861 cgcctgcaga accacccgtg cagtgcccgc catcctcttt catggcacag cagatgccgt
    13921 cgtaccttat gcaggcggtc caacacagcg ctttccctat gatttcgtaa acctgccgga
    13981 tttcgcgcaa gagctggcaa cgcgcaatgg ctgcacagcc actccacgca cagagcagat
    14041 cagccagaag gtgcattcca tcacctatac agcatgcgtc aataacgcag atgtggtttt
    14101 ctacagcatc gcagggggcg ggcattcctg ggcgggtgga agagccatgc cgcgcatcat
    14161 cgcgggcgaa accaacatgg aggccaacgc cacacgttta atgtgggatt tcttccagaa
    14221 ccaccctctc gttgaggatc attaatcttc ccggatctta tgatcgaatg gtcaattttt
    14281 ctgcaccaca agaaagccac ttgataagta taaggaacaa tgaacagcgc actgctcttg
    14341 ttgaccgtgg ggctgattct agtttttgca cctctggtat ccgcagagga aaagcaagcc
    14401 agggctggta acgcgtggaa gggaaagtgg ctggaacgag gttgaataaa ttatatgaca
    14461 gtgacaggaa tctcagcaaa tcctctgggt gcacattctt attgattgat ggcttgttcg
    14521 agcatggcgc tcaattgctg tttcttttgc tttaattgcc ctgccagaga gggcagcggt
    14581 tccaagatcg tcaatatcgt ggtcacatat ttatttctct tatacgctga gcgctgcaca
    14641 aactgcgcga attcgatcaa ttcctcggca cttgaataac ctggaatatc cttttcaata
    14701 tagttcatcc agtcgtccca gtacgaaaca cagagcctgg aaagttggac agggaggttc
    14761 ttctctttct cataatcaga aaagtatttc ttattaagcg cttctgaata aatagttggg
    14821 taatactcca ggaaggttct gcggattttt cgtatctctt tctcgatctc tgcaacctga
    14881 gatttgagca gctccatggc tgccatagaa aactgtatct ctttcaactt ggctgatgcc
    14941 tgtagatcgc gcgcctggat ggtgctgaaa acgccatcgc tgtcctgttt gagcaccagt
    15001 tccgatccgc agtacataca aaaatatttc tgtaaatctt tgcgcacctg cagttgccct
    15061 ccgcacgaag gacaggtcat cgaaataaaa tcattcatga tttcctccaa agataatgct
    15121 tcattgattc tgaacggctt gctcaagcat ggcgttgagt cgcgctttct ttgccttgag
    15181 ctctcccgcc agagttggca aaggctcaaa gagcttctgc agcggcaaca gaaccttatc
    15241 cttctggtat ttgggctgct gcataaatgc atagagcgtc aaaaaatcat ccacgctggt
    15301 gtaacccatg atatcccacg ccggattgaa ccagttgtcc cagttgagga cgcagaattt
    15361 attgatgccg ggtgtgatga aattttcttt ctcataatcc ttgaaacatc tggctgtccg
    15421 caacgcgata acatcggtca aaaaggcatt gcgtattttg gcgaagcgat cttccaattc
    15481 cgcgatctgc ccctttaaca gatccatggc tttttcagaa aactgcatct ctttcaactt
    15541 tgccgaagcc tgcaggtcgc gcgcttgtag ggggatcaac atcccactat catcttgttt
    15601 gagcagcagt tgcgtgccac agtgtgtaca atgacacttc tgcaaattat tttggacttg
    15661 cagttgccca ccgcaagatg ggcaggtcag cgcaatgaaa tcgttcatgt atccctccaa
    15721 aaaatatatc cattaatttt acgtctattt tggaaatgga gaacacaaac attagaacag
    15781 aaagaataat gatccagaat aaaaattacc ggttctcatt ctaatcagct acagaatcag
    15841 gcaagcacaa accgttgaac accaaaaacg tcacaacccg ccgaagatcc ttcaaaaacg
    15901 tatggatcct ttttacgcaa ggtttgatag cgatcaacta ctcgctgaaa tgcagcctgc
    15961 gcccgcggag attgttcacc cagtaccgca accgttccac cagctccccc accggttatc
    16021 ttggcaccaa acaacccttg ttctttttct gccctcacta attccacgat cgtatcagta
    16081 tattctgaac ctaaaccgca atcggtgtac ccttgatgag attggtacat cagctcaccc
    16141 aataaacggc atgttgtttc atcgactaaa tccactggtt tggagattag actgcaaaaa
    16201 gcatgcacac gccaattctc ttccactgca tatcgcgtag ctccgcgcac cgggtattca
    16261 atttcattcc gcactgaggt gaatggatcc agatgaattg cgaactgctg cgtgaagtga
    16321 ctcccttctt cagacgcagg tagacgttct tcgtacaatt gtgaaaagag agagggtgag
    16381 aggttggcta aataaccatt ccatttggtt tccacccaac gcggtaaagc cccgctttga
    16441 tcaagtgtag gctgcaaatc ttcccattcg cataagtatc gataccccat aaaagtggct
    16501 gcgcgagcag attcgtattc gattcccgaa accgcatgtc gtacgtctga atcgacgccc
    16561 cacacatgca aattctctgg caatctcacc agttgatatg gctggcaagg ttggcacagc
    16621 attggcacaa aataaccttc atctcccatt accacagcca gttgatccat gatgccacac
    16681 gccgattgag tgagtgcgat ctccacccat tgcgtccacg tagccagttc cacaccactc
    16741 acctttatct cgtaacaagc ggccattcct ttcatgccag ccacctccag ggctgcagaa
    16801 gagctgacac cctttccaag cgggacgtct gactcgaggt ataaattgaa tccatgctgc
    16861 acttttgctg gaaaatgttc tagtaaataa tacagatcac ctattaagta tgcgcaccat
    16921 gaacgatgcg ggtctgcatt gatccaatca cgcacttttt ccatcggttt gacatgctcc
    16981 ccctccataa ggtctgtcaa ggaaaagctt atgaaatctt cccaatctaa atttttcacg
    17041 gctgggttat aaaaacgaaa cagcatgtcg gtacgcggct ggacagcgac catggtcgct
    17101 tctcggatag ttttttcaaa gaccaaacca cccgtataat cgtcatttcc acccatcaga
    17161 tccaacctgc caggggtgcg gttaatgaaa atatcctgtc tgctagcaaa gaagccatct
    17221 gcaactaatt gatgaattct ttcttcaaac tttttcagct ccggatactt ttctttgaat
    17281 tcatgaaaaa ctgaacacat aaataatgct ccctttggat tgatcgacac acctggatgt
    17341 tatgaaaagc attttagcat aatatattcg aacggtcaaa cgtaatattt tataacatta
    17401 atattgacaa acgaaacttt tcccctataa ttgttaaaga cgagacaatc tacatagaaa
    17461 gcacttcctt ttttctgatc tctagcaagt ttcgcacata ccgaggaagg ttgtaattgt
    17521 ttgaggagga tgtattacat caggaaaaat ttgtttcaca tccaacgtgt agttcgtaaa
    17581 acaaagactc gcttcaaaac aatcgaagcg ctgattcaaa cgaaaaaaga gttttaaagt
    17641 aaaagtcttc ttgtaaaaga acaattcttc tttgctcttt tcaaacaaat aggcacaaaa
    17701 gcgatcaagt aaatggaaat tgcatattgg tagggaatac caatatttac accgagaaca
    17761 gatctgaaag cttgctcaaa gattttgcga tcattttaaa aagatgaatc aaaagaaaag
    17821 cacgcacaaa ctagaagcaa tcattgttgg gggaggcact gcaataccgg tactcgatca
    17881 ttcccctccc agtctatatt tgaaattcaa aaatctatca gcactgctag atgtaggacc
    17941 gggcactttt tccaaacttc caatatatgg cataaaccct ctcctaatta ataatatatt
    18001 tgtcagtcat ttacatcctg atcatgttct tgatttggtc atctattata tgatccgtga
    18061 gaatgcggca aagaaaaaga actctcctgt aaatgtcatt ggatgtaaag ggataaaaaa
    18121 ttttatcctc cagttaaaca gaatattccc tgatatttca ttttcaaacc ctgcgattcg
    18181 aacccatgaa atggccaatg acgaatatat ccttggagat attcgggtgc gcacaatcct
    18241 aacaggacac acaccccata gtattgcatt tcgctttgat ttcaacaata tatcctttgt
    18301 ctatacaagt gactgtattt acagccagca attggtggaa ttctgcgcca aagcagatat
    18361 tctggttggt gagtgttctt tctcagattc ataccctacc tccgatcata tgaacgctca
    18421 gaaaattggg tcactggcag aaaatgctca agttcaggaa atgtttatca ctcattgtta
    18481 tccacaagct ttgaaaaaag atctaaaaaa acagattagc aaatatttct caggtcccat
    18541 tcacatcaca caagatggag atcgcataaa caggtaaaca catgaaatac gccacgcagc
    18601 ttttaaataa atccagcata aaaacaagag gactcaactg gtttgccctg atacccctgc
    18661 tcattttctc aattggattc cagataattc ccatcatttc actgatcagt tctagcttct
    18721 cgacaaaaga aggattgtcc atcgagaact accaacgtgc cttcacaccg cacatcgccg
    18781 aagcttttcg gaactccctc aagctttcat ttacaacagc atttcttggt gttggtttgg
    18841 gtttggtggt cgcttacgcg atcatctcga cagagaaaaa attcatgaaa aatgcactca
    18901 ttgctctggc tgatgtaaca acgaattttg ggggcgcgcc tctcgcgttt gcttttattg
    18961 taacgttagg atctacagga gtgatcacgt taattctgaa gagcatgggg gttttccttt
    19021 atcccagttt tcgaatttat tcgatatctg gattagcaat tgcatatctc tattttcaac
    19081 taccgttgat gatcatgttg atactgcctg ctctgcaagg attaaaaaaa gagtggtggg
    19141 aagctgccat caatatgggt gcaaattcaa tgcaattctg ggcacgaatt ggattgccag
    19201 tgctcacccc ctcattattg agcagtttct tcatactatt cgcaaactca tttggtgcat
    19261 atgcaactgc ctggactctc accggaccgg atgtgaatct gattacggtg caaattgcgt
    19321 ctctaattag gggcgaagta cagcttgaaa ttggcctggc agatgcatta gcagtcatct
    19381 cactatttat tattctgttc tgtgttggag ggtatttgtt gttatccaca aaagccaaga
    19441 ggtttcgaac atgagaatga ataaattgct tcgttcaatc gtactcgcag tatttctact
    19501 gttcatgatt gtacctttaa tcgccacgct cctcttttca atgtccacac gttgggatcg
    19561 cacaattctc cctgaagggt acactttgga atggtggaaa accgtcattt ctcggggtgc
    19621 atttggttta acgctcaaga attcctttta tatctctttt gccaccgcta ctgcattatc
    19681 gatactagta actcctactg catattgggt ccacatccga atgccaaaaa acaaaatcat
    19741 tttcgaaata atgtccgctt tttcctttgg cattcccggc gtcattctcg ccctggcact
    19801 tattcgtttt tattcgggcg tgccgttacc tctgataaac acaaccaaca tattattgtt
    19861 ggcttgtatg gaattgtgtt tcccatttat gtaccgtcca gtggcaaact cgcttgatag
    19921 catagatatc catactctaa ccgaagcgtc gcaaagtctg ggcggtaact ggtttgccac
    19981 atttatccac atcattcttc caaatatttt accgggaatc gccagtggat tcctgttggt
    20041 tttttcaact gtattcgctg aattcacatt gacaaactta ttggtagggg ctcgtttcaa
    20101 aacatttccc atctatctcg ttgaattcac aagatttgac ggaagacagg ccagcgcctt
    20161 agccattatc agttttgtag tggcatgggt tgtatcaacg ttgctgttat tggtcagcgg
    20221 gaagagagcc ttatcacctg acaatttaac gattggacat tagaaaagaa aaggaggtgc
    20281 ttcacaaata gaacacaagg aattcatata cagcattaac aaaaataaaa ggagaaaaaa
    20341 tgagcaagaa aacaatcact atgtttttac tgttaacagt tttaatcacc cttggtgctt
    20401 gtcaaacatc agtagaagaa gaggtagctg agggacccgc cgctgaagaa ccaattacag
    20461 aagagcccca agtttcagaa aaagagttaa tgatcgaagc agcaaacgaa gaaggagagt
    20521 taatcagcta cggtatgtct gatgattggg tcaatctggg aaatatctgg aaagccattg
    20581 aaaccaatta cggcgtagta catatggata ctgatatgac cagcatggaa cagatcactc
    20641 acctcttggc tgaaatggaa gcgcctgtga tggatgtggc agatattggc ttcgattcat
    20701 taggtttatt gctcgaaaat gatctggcaa tggaatataa gagtgaatat tgggataaat
    20761 ttgacgagga ctttcgcgac ccagatggaa aatgggcagt tggatattgg ggagccattg
    20821 catttctggt gaacacagat ctggttgaaa acatcccaca gacctgggct gatctgttaa
    20881 aacctgagta tcatgaccaa atttgcaacc gcgatccacg cgtttcaaca tatgctaccg
    20941 gcgccgttct ggcagccgca tatgctaatg gtggtggtga agacaatgtc caacctggac
    21001 tggatttttt tgaacaatta cgtgattcag gaaactggcg cgaaggggtg gttctcaatg
    21061 tcgcctcggt gcagaaaggc gaatgcccaa tctcaatcgt ttatgacttt gatggattca
    21121 acaaacgaga tgccactgga ttgcctctgg aagtagtgat tccggctgat ggaacagttg
    21181 gtatgctgtt cgcccaatat attaacaaac ttgcaccaca tccgaacgct gccaaattgg
    21241 ctattgattt cttctactcc gatgaaggtc agatcatgtt tgccgaaggc tacgcgcatc
    21301 ccggtcgtag tgatgttgta ctgccagatg acgttattgc caaacttctg cccgaatcgg
    21361 cttacggtga acttcatttt ccatctaacc tggaaaactt ctcagcggcg atgcagcaaa
    21421 tcaaagatgg ctgggaagct atagtaggag aatcagaata gatctaaaaa cgatcagggt
    21481 atcaatgatc tcctctgaaa tcagatagtc cacaattcaa cgaacactgg cgagtcaatg
    21541 ctcgctagtg ttctcctaaa attctaaaca gtttttccgc actattttta tcattatttt
    21601 tcatacaata atcaggggat accaattatc ataacatata cgaacggtca aacgtaacaa
    21661 tttataatat taatattgac tttcgaaaca taccactcta taatactctt accaatggaa
    21721 aaaacacaca gaaaaaaaga gattcgaaaa tggatggggt tcgtgccatt tctagcagtc
    21781 tgcctgactt ttgaattcgt tccaattttt tttctcatta gaggcagctt tcttgataag
    21841 gcaacccaaa gctacacgct ggcacattac caggcactag cgcgaacagt ctacgttaat
    21901 tctttcataa acagtatcaa aatctcgagt ttgacagcca ttataggagt ggtgcttggt
    21961 acattgatcg ggtatgccat ttttcgttta ccgaaagaag gttttaaaaa tgcgctcatt
    22021 tccctttcag acgttacgac caactttgca ggggcaccct tagctttcgc gttcatcatc
    22081 attcttggaa taaacggggt tatcacccag tttcttctaa agtatttcaa ttatgaacta
    22141 tatccggctt tcagcatcta ttctttttca ggcctggtcc tagcctacac ctattttcaa
    22201 ttgccattga tggtgctgac catcattccc gcttttgttg ggatcaaaaa agaatggcaa
    22261 gaatctgcag aaagtttggg ggcaaacaag attcaattct ggtggcatat cggcattcct
    22321 gtactaaccc cctcactgct ggcaggtctt acattgctct tcgcaaatgc atacggagct
    22381 tatgcaacag catatacatt ggtcgagtca cagctatcac tggttacatt gcaaatcggt
    22441 ttcatgatcg ccggagaagt aaggcacgat ccgggggttg gtatggcaat gtcagttctt
    22501 tccttgataa tcatggggtt gagtattgtt gtttatcaaa tcgcgacaac tcgagtacgt
    22561 cgttgggcaa aatagaatga atcaggacac atatgacaaa tcgaagaaca tccacattta
    22621 aaaaagcaag aacccatcta caactatggc aagtcgtctt gattgcactt tttatcttct
    22681 atatgctatt acctattttt tctacctaca ttttttccat cgcaacgcga tgggaccgca
    22741 caattttacc cgaaggtatt acttctgaat tctatcaaga agctacaaaa gcttcatatt
    22801 tttcttcgtc attgcgcaat tcgcttattc tctccctttc aaccattctg gtcagtctgg
    22861 tcatcattat ccccacagtg tattgggtaa acgtacgttt gccgcaagcc aaatctattc
    22921 tagacatatt gatgatctta cccttcggca ttccaacagt ggttttggca ttggccttgg
    22981 tgcaggttta taacggaacc cccctaggtc gaacaccgca attattaatt ggtgcagttt
    23041 tcatctattc tatgccattc atgtaccgac ccgtctccaa cgcaatgcga tctattgatg
    23101 cactcaccct caccgaagct ggtcaaagcc ttggcgcttc gactctgcag gtgatgattc
    23161 aagtaatcat tccaaatatt ttttcaggaa tcatcagcgg cagtctgtta gtttttgcaa
    23221 cagtatttgc tgaattcact ttaacaaaat tgattctggg agcgcgtttt aaaacctttc
    23281 cgctcttatt ggtggaatat acgcgcatca acggcaacat agcggctgct ttttcggtga
    23341 tctccttcac aattgcatgg ctgatttcct tactaatttt atggatcgga aaaaaagggc
    23401 agggttcttc tactcaggca ccaaccgccc attaagaaaa ggagtacaga atggcttacc
    23461 tggaattaag aaatattcac aaatctttcg gaaacgtcac agcagttaat aatttcaacc
    23521 ttgacatcga aaaggggaaa ctagtttcct ttcttgggcc ttcggggtgt ggaaaaacga
    23581 ccacattacg catgattgca gggtttgagc aaccagatga aggcacaatc cggctgggca
    23641 atcaagatat aaccgttatc agtccgaatc agcgcgacat aggtatggtg tttcaggcat
    23701 atgccctttt tccgaacatg aatgttcgtc agaacatcgc attcggatta aaaatgaaaa
    23761 agacaccgaa agctgaaatt gaaaaacgag tagatgaagt tctagaaatg gtgcgcttaa
    23821 aagatactgc caaacgtttt ccgcatcagc tttcaggtgg gcaacagcaa cgcatcgctt
    23881 tagctcgagc gttggctgtt cgaccgcgcg tattgcttct tgatgaacct ctttctgcac
    23941 ttgacgcaga agtacgcgta gcattacgtg gcgagatcag gcgcattcaa tctgatttgg
    24001 caattactac agtttacgtt acccacgatc aggaagaagc actttcaatc tctgaccagg
    24061 tcgttgtaat gaacaatggg atcatccagc aagttggtac ccctgaggag atttaccgat
    24121 caccggcaac ccgattcgta gccactttca ttggaacagc caatcaattt ttcggaaatg
    24181 tagcaggaga atgtaaggtt cagagcaatg gtttaaccat cacaactcgc aaattatctg
    24241 atttttcctc cgggcaaaaa gtggtcgtac tggtacgtcc agaactgatc atcgtaagca
    24301 acgcccagcc tgatacaaat tcagggaata ccatccccgg catggtggaa accattactt
    24361 ttcacggtgc agtaacacgc ttaggagtgc acgtgaatca acaacgagtc gttgcagata
    24421 tcacaatgac ttcctcggga tatatctcgc tgaatcaaaa agtgtggttg tccttcaaac
    24481 cagatgcatg caaagtaatg gcggttgatg aatgatctac atcaatgggc atgagatcgt
    24541 acttgataaa caggaacagc tggaagcatg gaccagctat ggtcaaattg tcaagctggc
    24601 tatggatttt atacaatcct gccctcgaga tccacgcaac aatctaccgt ggtacttgca
    24661 atattcttgc ttctggacag atccactgcg ccccgctgat tggcccgata atccggctgg
    24721 aaaattcgcc tgggctgtaa cagccatact taaatattac ccatattcag ggaaaaaaga
    24781 ttttgtagat attgcggtcg acatgcttga ccatctatgg gatcaacgga cacctgattc
    24841 cttcctttgg aatgaggttc catatgcctc agcgcatcct gggacaggta tctactttgg
    24901 agcacgtgca gatggagaat acgtaaccga acccgataag atcgctcaag ttggaaaagc
    24961 ctatcttgac ctgtacaaac ttactgtcga agaacgattt ctacaacggg cggagcactg
    25021 cgcccaaatc ctatccaaca atctccgtcc gggagaccag gatcattccc ccctgccctt
    25081 ccgcgtgaac gttcagaccg gaaatattgt tgaagaatac accgcacata tgatcaccgt
    25141 agtccggtta ttcgatgatc tgattaacct gggcaaggga aactatcaaa aaaaacgcga
    25201 gagtttgttg ggttggatac aacgctatcc gcttcaaaat aacaaatgga aaggttattt
    25261 tgaagatatc agggtcgatc caggtaatga aaaccgtgat cagctttctc caatcgagac
    25321 cgccagatat attctagaac atcgctcaca attcccccac tggcaaacaa tggtccaagg
    25381 attaattgaa tgggtgcgag aaactcttgg gagccaaccc tttttctctg caatcccgat
    25441 tcatgaacaa aagttttgct attttgtcat gggcagccat acggcccgtt ttgccagcgt
    25501 atgtgcccaa tttgcagacc tcacagccaa tgtgaaatat cacgaaatgg cagttcgttc
    25561 atttaattgg gcaagttata tggcaaatgt ggatggaact gtgaccgttg gaatcgaccg
    25621 acctgactat tacaaccagt gttggttcac ggatggatac tttgactacg tgccacattt
    25681 tttagatgga atggcatctt taccagattt ggcacctgat gacgaggatc atctgcttcg
    25741 ttccacttcg attattcaag atatctccta tcaaacaaat gaaataagtt accaaagtta
    25801 tgatgcaaac ggttgtcagc gtttccgatt aaccttttta ccaaaagagg tattagcaga
    25861 tgacaaacca ttacaaaaga aagctgaacc tgacgagaaa ccaggctggt cagtaggaat
    25921 ggtcccctat gtggtagaca tctattccgg tgcacgaaat gtgacgatcc gaggttaatt
    25981 cagaaatgta tataaaaaat tacaaggagg tgtccataaa acaaatcatt tcgataacac
    26041 accgttgagt gaaaagtaaa taatttataa ggaaaagaga aatgtctaaa aaatttttaa
    26101 ggttgatcgt ttttttggtg ttgttttcaa taattgccgc tgcatgctcc acaacctccg
    26161 ctggtgaagg ggcagcggtt gaagaaccgg cagtcggaga agcagccgaa ttagatccta
    26221 tggaacaact catcatagat gcaaaaaacg aaggtgtcct tgtttcttat ggtcttcctg
    26281 atagttgggt gaattatgat gaaatgaaaa cgttgctgct cgaaaaatat agtattgaaa
    26341 ctcaggacac agacatggga tcaggtgaga ttattgctgc attgggagcc gaagctggag
    26401 cgcccgtagc tgatattaca gatctcggtc taaacttcgc gaagatcgtc gaagacgaag
    26461 aattgtccca accttacttc cattcgtatt gggatgaact ccctgattac gcaaaagatc
    26521 cagagggacg ctggtcatct gcattttggg gtgccattgc aatcttggtc aataaagatg
    26581 cagtaacaac agtaccacaa acctggagtg atcttctcaa tgacgaatat attggaaaag
    26641 tctgtataaa agatcctcgt tcttcagcta
//
LOCUS       DAOSDS010000023        25093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_891_length_25093_cov_5.873193, whole genome shotgun sequence.
ACCESSION   DAOSDS010000023 DAOSDS010000000
VERSION     DAOSDS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 25093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25093
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_891_length_25093_cov_5.873193"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2377
                     /locus_tag="PLL88_04395"
     CDS             <1..2377
                     /locus_tag="PLL88_04395"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00835.1"
                     /translation="AERAIPELEALNEPLEKINPSLPRSLAQKAGIELLALENQESMR
                     ILLNELKLSEQDIPRLQHILNAGIQHRVLNALKHDEESQIIADAGAFGAVTIATNMAG
                     RGVDIKLGGELDEQILTDVVQVLKASGVDPYGMPLKDIESVLQKLPREGYAEKADSVK
                     EFLDYMINLVKVREIGGLHVIGSERHEARRIDNQLRGRAARQGDPGSSRFYLSLEDDL
                     MRLFGGERVENLMNFFKFDKSVPIENRLIGKMVEQAQERVEGYNFDIRKHLLEYDDVL
                     NDQRTRIYGERDKAITKADLSEDIWGMVQSELERRVPISLEESQSPWKLLAFLDEIQP
                     PMLNQELGVHIPSYSYHLIAQHLLPEMQKMHDSEGKSAVLKDFAANVIQAEETFVAGS
                     ISDLIEHTSENFESQLAERFENLDTFLDGIATDSPQGVDERDIRSQLQSYTHTRLQLA
                     QNEIHQLLDGDREMIKSLKQQVKASLFSIYLNRTGFTINKRLGDIINLDALKVDVGDW
                     DAVKDFYRDEIKKRFSEKRNALQAEQSELGRNIQEAARRLDAQELSEKQISTALFNIT
                     AGKRVAINPQNHQRVIKNVTLLNVVFYAAGLFDGKKPEAVIQDILMHLQATSLTLQKI
                     FGAYELNRMAQSNATIQQLNPALREKVVSILPSQDQESLLQKPVQELSDELREKMKTA
                     LGSEIQNMLHRHVLLTNINSLWIEHLTQMEALRVSIGLESYAQRDPLVQYKSRSTDMF
                     GDLLSNIRLGVMSQIFRLQPVQRPPASQSNAEQKPAGSQSDEKKKKRRRRR"
     gene            2480..3763
                     /locus_tag="PLL88_04400"
     CDS             2480..3763
                     /locus_tag="PLL88_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOJ00836.1"
                     /translation="MAQNDAKTPSGVDNSKNSDDKNLNMASLLEREGLGIELPKTGEI
                     RTGTIASISPGQILVSVGAKSEGLISGKEFELIPPEEFASLEVGQEIPVYVITVEDSN
                     GNLLLSYVRAVEESSWDKAEEVFKNKKTYKGKVAGYNKGGLLVAFEKLRGFIPSSQLS
                     YERLKEAAGNTPEERYGKMIGSDIEVSVIEVDRERHRLILSERLACGETRDTIREKVI
                     SDLEIGSVLTGKVTSVASFGIFVNVNGADGLVHVSELSWDEISDPSEKFKVGQTVKVK
                     VINIDEDKKRIGLSIRQLEDDPWLKNVENLKVGMLVEATITNLTKFGAFAKIQGDIEG
                     LVHISEISDSHISHPKEVLHDGEKVTLRIIKIEPESHRIGLSLRRVESAAYADMDMKL
                     LKQELEGSDIHVSGGDDEKTNSEDAEGSDATPEGE"
     gene            3854..4369
                     /locus_tag="PLL88_04405"
     CDS             3854..4369
                     /locus_tag="PLL88_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00837.1"
                     /translation="MPQNEPVLVEKPYQVSDESFIKRLRNFLVLTFVGFLIFLLGIRP
                     DLFHMDRSPVTGFIQISVSVVGLGILCLGGYLCMHWLWRGERPSIAAEIGVRFISTGL
                     VIAVFSAVADFFGFGSHPYPESLPYLGEWQVLGIEIGEYTIALGFILMIPFPKIQLFP
                     RLPQSDKPNRR"
     gene            complement(4346..5668)
                     /locus_tag="PLL88_04410"
     CDS             complement(4346..5668)
                     /locus_tag="PLL88_04410"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ00838.1"
                     /translation="MNQKTVAVIGAGPAGLFAAKTLAEKDVKVALFNRDVRPGGLAEY
                     GIYPEKTSLKKGLRRQFMSFLSHKNITYFGNMEIGENKDLSLAQLRAMGFQGILIAAG
                     AQKLRELKVEGEFLPRVYHAWEIVSKYNDLPPYSQIDYPIGKKVLIVGVGNVMADVAR
                     YLLQKGDVEEILVIARRGPAEIKFHETEFAHIAQNLDRADFEAEMERVTSLMLKLGQD
                     PNATWAFIEKACENCDEKTSSANLRLRFLKSPVQFLGNENEGLQEVKCVENTLVEENH
                     VMKTVPTSLHALFDVDTAILAVGSSVDDEMGLPVSDDLFTVSPYPDYPQDGISYEVMD
                     PVLEKNISGIFLAGWSRLASYGLVGISGKDGMKGAHAAYEFLQEQDIDCPDWQVLESK
                     ILDSKKTVVNKNMVDQLYALEAEIAQKKGLADYKFAANQEMLAAVGLV"
     gene            5766..7067
                     /locus_tag="PLL88_04415"
     CDS             5766..7067
                     /locus_tag="PLL88_04415"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ00839.1"
                     /translation="MQNDISSFQLSNGLRVLLKEIHTVPIVSQWTWYRVGSRNEKAGI
                     RGISHWVEHLQFKGTEKFPGEKLDQVISRVGGIWNAMTHLDWTTYYETLPANQIDLAL
                     DLEADRMQHSVFDEDEINAERTVVISELEGNENDPLFRLNSAIQSKAFQFHPYSHDVI
                     GTLEDLQHMTRDQLFRYYQDHYNPDNAILCIAGDFNITEVTAKIERYFSSIPPRSISE
                     ENIPVEPDQTNENYLEIKGPGDALYIQIAYHASQANHRDFFTLSVLDSLLAGPSPLVM
                     FGGGGISNRTSRLYQALVEKDIVVSVSGDVQATIDPFLHTFILTLNPTADPHKALQAF
                     DKEIEKIKQEKISRQDIDRAIQQAEAMFAYGSENITNQAFWMGYSSIFASYDWYENYI
                     QNLRSITDTDIQTFAQKHFAFHNRTIGIYFPTQEEDHDAIQ"
     gene            7051..8340
                     /locus_tag="PLL88_04420"
     CDS             7051..8340
                     /locus_tag="PLL88_04420"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ00840.1"
                     /translation="MMQSNNTNFQNHFPNASTIRRFQLGNGITVLAYSNMHSKSLTVI
                     GMLATGSAWDQAGKLGTAHFTASMLMRGNEKMTFREISDRLESVGASLSFSAGTLNTW
                     FQGRSLVKDFPMLLALLYDNLTSPIFPQEYITRLRNQLITSLLIRDQDTQERAALEFD
                     KVLFKSHPYALPVDGYAETIANIQRHDLTKHHSMYHPHNAVIAVSGALPLQDIESMIK
                     GQFDQWAPKKESVNPVSTGFEAIIEETRSHIPIEGKSQSDIQMGTFGPSRASADYYAA
                     YLGNHVLGQFGLYGRIGRSVRNKAGLAYYAFSSLNALPEIGSWEFNAGVNPVNVEKTI
                     TLIKDEIRHYLEEPLQEDELEDSKSHLIGRLPMSLESNAGIANALLSIERFHLGLDYF
                     QNYASLISSITPAQILEASRRYLDPEKLIIISAGAEK"
     gene            8337..8708
                     /gene="acpS"
                     /locus_tag="PLL88_04425"
     CDS             8337..8708
                     /gene="acpS"
                     /locus_tag="PLL88_04425"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOJ00841.1"
                     /translation="MILRSGVDIIEITRFEEQRPEIRKRFLARVFTVRELSLIRNSHI
                     SAAGRFAAKEAVAKALGCGIGKVRWQDIEILQGKDGEPLLILHAKAEEISQQAGLKTW
                     SLSISHSRQYAIAFAVAVGTS"
     gene            8721..9380
                     /locus_tag="PLL88_04430"
     CDS             8721..9380
                     /locus_tag="PLL88_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00842.1"
                     /translation="MTEKILIVSDKISEQLYDPSKQKALKGIRFIVSSGDLPYYFLEN
                     LVSTYNVPLYYVRGNHASFYESETGVIHDHPWGCIDLHVRSVKDPSGFLLAGVQGSHM
                     YNNGPYQYSQAGMWQFVFRLIPSLMANFVISGRYLDVFITHAPPWGIHDGQDIAHQGI
                     KAFLWVLKVFKPLYHFHGHVYDFGKNSPMISQYENTQVVNTYGYRVHEISMNKKPATN
                     D"
     gene            9389..10297
                     /locus_tag="PLL88_04435"
     CDS             9389..10297
                     /locus_tag="PLL88_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00843.1"
                     /translation="MSFDSFKHQSKADFDEAYLHGFIRSIENWLRFEKTQLLAFNEIL
                     KRISVQSQYDAGVQEIEIDAIVGSVNRYADFDNAFLPRQKHTRSRWEKIDRAYLREEI
                     LPPVDVYQVGNFYFVIDGNHRVSVAKQRGQKWIDAHVVKIETPYEVDRSFDFNQLILK
                     EEQNQFYEKTNIKKSYPDANIEMSIAGLFERLQEHIDAHRYFTGEFLQREISYEEAVS
                     SWYENIYLPLVKIIRQQKILDQFPDRTEADLYFWIIEHLAFLKAEYHKDFSFEEAAGD
                     FLMKFHPGFIKLTVAKAKHWFKKKRR"
     gene            complement(10294..10521)
                     /locus_tag="PLL88_04440"
     CDS             complement(10294..10521)
                     /locus_tag="PLL88_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00844.1"
                     /translation="MEKGMLWFDDRKDISIEQKINAAVLFYQQKYGGKPDRCTVNPTV
                     TAGAISKKLAGLRIEVSQYILPNHFLLEEDR"
     gene            10670..11227
                     /gene="frr"
                     /locus_tag="PLL88_04445"
     CDS             10670..11227
                     /gene="frr"
                     /locus_tag="PLL88_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOJ00845.1"
                     /translation="MINEAIQDAEKRMKSAVQTLEEDLSGIRTGRASPALIERLSIEY
                     YGSPVPLMQLATISVPEPRQLLIKPFDATSIKDIEKAILASDLGLTPLNDGKIIRLNI
                     PPLNEQRRVELTKLVGNRCEDSRIAIRNVRRDIIKDLRDFEKEKLISEDDLKRGEEEL
                     QKLTDKMISEVDSIGEQKEKEIMEV"
     gene            11237..11983
                     /locus_tag="PLL88_04450"
     CDS             11237..11983
                     /locus_tag="PLL88_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOJ00846.1"
                     /translation="MAKQKELTFEKVPRHIAIIMDGNGRWAKARMLPRMAGHRAGAEN
                     LRRIITACVDFGVRYLTIFAFSTENWKRPADEVNGLMEIFADLLEKEVPKLHAQGVQI
                     RHLGSIQELNPTLQRKLQNAIQTTQKNERLILSIALNYGGRDEIIHAVKEIVKKGTPA
                     DAIDEQTIKDHLYTRGMPDPDLVIRTSGELRTSNFLLWQTAYSEWLFTETYWPDFNKD
                     HLQKAILEYGTRSRRFGGRTKKEEEETYNG"
     gene            11976..12782
                     /locus_tag="PLL88_04455"
     CDS             11976..12782
                     /locus_tag="PLL88_04455"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOJ00847.1"
                     /translation="MAKRILGIAIIAPIGVGLVIAGGWIFTLGASAILGIAAWEYWNF
                     FQESKFAPHKYFLIVATVISAVARYLFNGAYFEAVFALLFIASIIISLIRYEKDDANS
                     LATMGLEWIGLIFIAYFGSYLISLRFLPDGKMWILLTVPAIGCGDIGAFLIGTYFGKH
                     KMAPRVSPHKSIEGYFGGILFTVLYALLFSLIFTFGAANITVGRSIMLGLILGIISPL
                     GDLLASLLKRSFNKKDSGKIIPGHGGILDRVDTWLLGAAAAYFIIRFFWI"
     gene            complement(12869..13609)
                     /locus_tag="PLL88_04460"
     CDS             complement(12869..13609)
                     /locus_tag="PLL88_04460"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOJ00848.1"
                     /translation="MKKHYLIAGLSALMVVLLAACSGTPSASAANTPVQKSITVTGTG
                     VVKVVPDVAYINVGITTQNDNVTDAITENSNQAQTIKDELVALGIAEEDIQTSSFSVY
                     PQSNYDYNGNITGTTFVVNNSVYVTVRDLSSMGDLLGKITESGANSIYGISFDVQDKS
                     AALTQSRELAIELARQQAEEVAESSGLKLGEVLTISMYSSDVPYLVTDSYSMGMGGGG
                     YSQSAATVPISAGNYVITSSATIQYAID"
     gene            complement(13773..14219)
                     /locus_tag="PLL88_04465"
     CDS             complement(13773..14219)
                     /locus_tag="PLL88_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOJ00849.1"
                     /translation="MKDRLYRSSQNKIIGGVCGGLAEYFQIDPIFVRIFLILWLIVGE
                     WGVFVYLLLWLIIPPQQYEGAGFQLNDLGARFRLIGDDLRDLFQNPSAQLLTFGGIVL
                     IGVGLSTLARALGWDWGQYWNSTLVWAGLLIAGGIFVLVKTYGKKK"
     gene            complement(14237..15025)
                     /locus_tag="PLL88_04470"
     CDS             complement(14237..15025)
                     /locus_tag="PLL88_04470"
                     /inference="COORDINATES: protein motif:HMM:NF021443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF-related protein"
                     /protein_id="HOJ00850.1"
                     /translation="MRNRIFWGAVLIILGVIFLFSYKFGFSIWSLIWPFALIVTGSWI
                     LLVPVLSQGMEVVKDELEVPLTEVDQVSIKMEHGAGTITLAAADLPSAILKGNFIGGV
                     NAKVSSADRRSTIKLESKVAFMNLLPRFQKEQRLMWDLVVNQTLPLKIKMETGASDNH
                     LDLRNAQLKELRLETGASSTEIFLPEKAGETKVHIESGASSIKIHVPQNVAATIKVGG
                     LIGKTIDTARFHRNGDVYQSVEYPNAANRVDIEIESGVGSIEIQ"
     gene            15321..15611
                     /locus_tag="PLL88_04475"
     CDS             15321..15611
                     /locus_tag="PLL88_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00851.1"
                     /translation="MQIINTITTLMGNIPLAWLNKISPSHSTRIAANLEHFYPSHNGK
                     DHSGFKTTPGAAISASLQVARRSEKQDELSMGIVPSFDEHRLGANPYSQDGD"
     gene            15620..16255
                     /gene="cysE"
                     /locus_tag="PLL88_04480"
     CDS             15620..16255
                     /gene="cysE"
                     /locus_tag="PLL88_04480"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016424288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOJ00852.1"
                     /translation="MFSEMKNDIRVALERDPAARNWVEVALCYAGVHAIWNHRIAHWL
                     WKRNLKLLARWASQLSRFFTGIEIHPAAQIGSGLFIDHGMSLVIGETSIVGKNVTMYH
                     NVTLGGVSLQKGKRHPTIGDDVVIGAGAQVLGAITVGNCSRIGANAVVVKDVPPDSVV
                     VGVPGRIVQKEHVEMHIPDLSHNQLPDVIAQQLLNLAQRLEKIENGLKDKG"
     gene            complement(16273..17187)
                     /locus_tag="PLL88_04485"
     CDS             complement(16273..17187)
                     /locus_tag="PLL88_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ00853.1"
                     /translation="MLIPISFVEADAISAYDLVVAVNTYRNLNSLNQLATNVYLMAAA
                     QQHATYLANTYGTTPPGESQGHVGEGGSDATERAKQIGYTIITGVQVREAWAGVDAST
                     SLETLLNSTWGDSNNAGVMLNQYASQIGAGMAQVDNIVYYVLNISVDYSIAPTSSTLT
                     AKTTETPGVVPVVVSTPKPDGSITHVVEKGQALWSIAITYGVTVDQIRILNNLSDNAA
                     IYEGQNLIVRPAFTSTPTLIPTNTPLPPTRTPIPPQTPQALNSQTGDSLQEKAMTSNQ
                     REILGIILIVICGGCLVYLIYTMLRKKG"
     gene            complement(17263..18240)
                     /locus_tag="PLL88_04490"
     CDS             complement(17263..18240)
                     /locus_tag="PLL88_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ00854.1"
                     /translation="MKKNWIVVSLVFLIMCLAVTRPVHAAPLLIPTAYELIDAVNAYR
                     AANGLAPLVIDSLLMISAQTHAEYLASQSGALTGHVGVGGTDADARAAALGYPQVPGL
                     DINENWANLPASADLDVLLFTAWGDSQHTHTMLHTRGQHVGAGVAVSGDNVVYVLNVA
                     AFWGDAGLTVQPTSGAYPGISGGNSTGASISQYIAPVEMAEPDESGKVIHQVLQGQTL
                     WSIATTYGVTIDHICQLNGLTSESMIYTGQKLLIKMVPTPSLVPTITIGIHLENQFPV
                     VTTTAPLLAAQPSTRENRQPLSDQWVIMGIIVVAIIGFALIFLGQRSSQ"
     gene            complement(18261..19031)
                     /gene="surE"
                     /locus_tag="PLL88_04495"
     CDS             complement(18261..19031)
                     /gene="surE"
                     /locus_tag="PLL88_04495"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOJ00855.1"
                     /translation="MHFLVTNDDGVTAPGLLALAQEIRKLGKVTVLAPDKNWSASGHV
                     KTMNRPLRVKETLLDDGTQALTSDGAPSDCVALIILGLLGEKVDFVVSGINPNANLGY
                     DVTYSGTVTAAIEGTISNIPSIAVSLDAAEYHGGPLDYRAAAQVAGMVAGRVLENKLP
                     KNVLLNINVPYLPLEEIKGYRITRQGLRVYRDALVRREDPRGRPYYWIGGDAPTGVPE
                     EGTDIGAVSAGYVSITPISLDLTAYDRMEYFRDWNIGN"
     gene            19228..20124
                     /gene="rpsB"
                     /locus_tag="PLL88_04500"
     CDS             19228..20124
                     /gene="rpsB"
                     /locus_tag="PLL88_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOJ00856.1"
                     /translation="MSANISMKSLLESGVHFGHRTNKWHPLMKPYIFTERNGIHIIDL
                     QQTVKYLDEAYDVIRDTVAAGGTVLFVGTKRQAQETIQEEAERCGMPYVVQRWLPGTL
                     TNWMTIYQRILELDNLENKRQSGQFDKLVKKEILMIDRRIKRLLKNLSGIRRLKNLPD
                     MLFVVDVMRESTAVHEANLKGIPVISLVDTNCDPNGVDYLIPANDDAIRAIKLLVGKI
                     ADAVLEGQDMRKEEVELEEASVIEGAPSAEAVRRQVALEEEISDEELLGASTLAKLTV
                     NKDDNPDDEYLDDDGDEEEEKN"
     gene            20148..20768
                     /gene="tsf"
                     /locus_tag="PLL88_04505"
     CDS             20148..20768
                     /gene="tsf"
                     /locus_tag="PLL88_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOJ00857.1"
                     /translation="MEITTEMIKQLREESGAGILDCRKALVDTNGDFEKAKVILREKG
                     LATASKRADRVASEGMVELYSHGGGRVGVMVEVNCETDFVARSEDFRTFAHEIALQIA
                     GASPLYVSDADIPEETIKDLETRATEKAKQEGKPDTIIAKIVEGSLKKYKDENVLLRQ
                     QYIRDDSLVIQDLLNQTVAKTGENIIIRRFARYTLGETSQNEEEGN"
     gene            20816..21532
                     /gene="pyrH"
                     /locus_tag="PLL88_04510"
     CDS             20816..21532
                     /gene="pyrH"
                     /locus_tag="PLL88_04510"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOJ00858.1"
                     /translation="MADVKYHRVVIKLSGEALAEDDGNGIDPKRAANIAKRVSDIHAM
                     GVDVSMVIGAGNLWRGREGLKWGMDRATADYMGMLATVMNALALMDALERIGVPTRVQ
                     SAIEMRAVAEPYIRRRAIRHLEKDRVVVFGGGTGNPFFSTDTAAALRAAEIGADVLIK
                     ATKVDGVYDSDPQQNHGAKKFERLSYIEALNLRLEVMDSTALTLCMENKLPILVLNLW
                     DDEALLKAIQGENVGTMVCD"
     misc_binding    21635..21881
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            21919..22962
                     /gene="pheS"
                     /locus_tag="PLL88_04515"
     CDS             21919..22962
                     /gene="pheS"
                     /locus_tag="PLL88_04515"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOJ00859.1"
                     /translation="MEEQKDLVVILQQIQQKALESLQQIKDADSLEHWKSTFLGRSSE
                     VMRVFKEIPSMPTEVRPALGQTANVVKTELEKQFETARERIQQAALANNLLAQKLDVT
                     LPGRPPERGRLHIATQTLQEIYRILAEMGFQVYRSREVESDEYNFELLNIPAHHPARD
                     LWDTFYTKKEGVILRTHTSPGQIHAMREYAPDPVRIILPGMVYRYEQMDASHEIQFNQ
                     IEMLAVGRNITFGNLRGTLEEFIKRMFGSGVETRLRPSYFPFTEPSAEMDMKCIACNG
                     KGCGVCSGTGWLEILGCGMVHPVVLKNGGYNPQEFSGFAAGLGTERISMLRHGMDNIR
                     NFWANDIRFLRQF"
     gene            22976..>25093
                     /gene="pheT"
                     /locus_tag="PLL88_04520"
     CDS             22976..>25093
                     /gene="pheT"
                     /locus_tag="PLL88_04520"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOJ00860.1"
                     /translation="MKLVLSWLKEFVDIDCSLEELARTMTMIGLEVENIQLVGLPIPQ
                     QEHLEHVYSGLTWEKDKIVVAEVSEVMPHPNADRLVLCKLNDGQGEQIVLTGAPNLFD
                     YKGKGPLSRPLKVAYAKEGSTLYDGHQPGKVLTTLKRMKIRGVESSSMICSEKELGIS
                     DEHEGVIILDDDAPVGMPLVEYMGDAVIEVSILPNMVRDACMLGAAREIAAATHKPLR
                     HPQPKLQGDGAKIEGEVKIEISDPELNPRFVFGMVRNATAQPSPYWVQRRLRLAGMRP
                     INSTVDATNYVMLEVGEPLHAFDYDILKKRANGHTPTIITRAAKQGERLTTLDNVERT
                     LDDFTVLVTDTAGPLALAGVMGGLESEITDVTRTILLEGASWNFIHTRRTVSAQKLQS
                     EAGYRFSRNIHPALAKLGVELCLSRIAEWSGGQIASGLVDAYPKPHIDPTIEITPQDL
                     QHLIGISLSPQEIASLLTSLEFECSIEGDHITVHCPPHRMDIGEGVVGKADLMEEITR
                     LYGYDRIPASRLADELPPQHANGQDDFEKQVKHVLVSVGLQETINYRLTSPEDEARML
                     LDQTALAGKEYVELLNPISQDKRVMRSSLIPQMLVNLEKNVRHADRLGLFEVGPIFLP
                     NKNEALPKEEYHLCLGLTGLEPKKSWDRKDSRKLDFYDLKGYVEALLEALHIQNAIFA
                     PAGHALFHPGKCAVLRWNDLELGI"
BASE COUNT         6810 a         6187 c         6073 g         6023 t
ORIGIN      
        1 cgccgaaaga gcgattcccg aattagaagc attgaatgaa cccttggaaa agatcaatcc
       61 atctcttccc aggagcctgg ctcaaaaagc cggcattgaa ctgctggcgc ttgaaaacca
      121 ggaaagcatg cggatattgc tgaacgaact aaaactttcc gaacaggata tcccccggct
      181 gcagcatatt ctcaatgcag gcattcaaca cagggttctc aatgccctca agcatgatga
      241 agaatcacag atcatcgctg atgctggagc ttttggtgct gtgaccatcg caaccaatat
      301 ggctgggcgc ggtgtggata ttaaactagg cggtgagttg gatgaacaga tcctgaccga
      361 tgtagtgcag gttctaaaag ccagcggagt cgatccttat ggtatgcccc ttaaagatat
      421 cgagagcgtc cttcaaaaac taccccgcga ggggtatgca gaaaaagcag atagcgtgaa
      481 ggaatttctc gattacatga taaatctggt caaagtgcgc gagatcggcg gtttgcacgt
      541 aatcggctcc gaacggcacg aagcccgccg catcgataac caattgcgcg ggcgggcggc
      601 tcgccagggc gatcctggtt cttcccgttt ttacctatca ctggaagacg acctgatgcg
      661 cctgtttggt ggtgagcgcg ttgagaattt gatgaatttc ttcaagttcg ataaatccgt
      721 tcccatcgag aaccgattga tcggaaaaat ggtcgaacaa gcccaggaac gagtggaggg
      781 ttataacttc gacatccgca aacatctgtt ggagtatgac gatgttctga acgatcaacg
      841 cacacgtatc tacggagaac gcgacaaagc catcacaaaa gctgatcttt cggaagatat
      901 atggggcatg gtgcaaagcg aactggaaag aagggttcct atcagcctgg aggaatccca
      961 atcgccctgg aaattattgg catttctcga tgagatccaa ccccctatgc tcaatcagga
     1021 actgggggtt cacattccgt cctattccta tcatcttatc gcacaacatt tgctgcctga
     1081 aatgcagaag atgcatgaca gcgaaggaaa aagcgccgta ctgaaagatt tcgctgccaa
     1141 tgtcatccag gctgaagaaa cattcgtagc aggtagtatc tccgatctca tcgagcatac
     1201 ttctgagaac tttgagagtc agttagccga acggttcgag aatttggata cattcttaga
     1261 tggcatcgcc actgattcac cacaaggggt ggatgaacgg gacattcgct cacagctcca
     1321 gagttacacg cacacccgcc ttcagcttgc acaaaatgag atccaccaat tgctcgatgg
     1381 tgaccgtgag atgatcaagt ctctaaagca gcaggtaaaa gccagcttgt tcagtattta
     1441 tctcaaccgc acaggtttta cgatcaataa acggttggga gatatcatta acctggatgc
     1501 gctcaaagta gacgtggggg attgggatgc ggtaaaagac ttttaccgcg atgagatcaa
     1561 gaagcgcttc tctgaaaaaa gaaacgcatt gcaggcagaa caatctgaac tcggaaggaa
     1621 tatccaggaa gcagccagaa ggcttgatgc ccaggaatta tccgaaaagc agatctcaac
     1681 cgcgctcttt aacatcaccg ccggaaaacg cgttgccatc aatccgcaaa atcaccagcg
     1741 cgtgatcaaa aatgtaaccc tgctcaatgt tgttttctat gctgcgggtt tattcgatgg
     1801 gaagaagcca gaagcggtca ttcaggatat tcttatgcat ctgcaggcaa ccagcctgac
     1861 tcttcaaaaa atatttggcg cttacgagct caaccgcatg gcacaatcca acgcaactat
     1921 ccagcaattg aatccggctc tacgcgagaa ggtcgtttcg attcttccct cacaggatca
     1981 agaaagcctc ttgcaaaaac ctgttcagga actgtccgat gaactgcggg aaaaaatgaa
     2041 aacagcgctt ggcagtgaga tccagaatat gttgcaccgc catgttctct tgaccaacat
     2101 caattctcta tggatcgagc acctgacaca gatggaagcc ctgcgcgttt cgatcggtct
     2161 ggaatcgtat gctcagcgtg atccactcgt tcagtacaag agccgctcca cagacatgtt
     2221 tggcgacttg ctctccaata tccgcctggg tgtgatgagc cagatctttc gcctgcagcc
     2281 ggtacaacgc cccccggctt ctcaatccaa tgcagaacag aaaccggctg gcagccagag
     2341 tgacgagaaa aaaaagaagc gccgcaggcg cagataacct ttcaaacgct catcgcagcg
     2401 cagaggggtt gccaaccaaa ccaatacact ttataatcga gctattcatt atcgcatgga
     2461 cggagtgatt gataatatta tggcacaaaa cgatgcaaag accccaagtg gggtagacaa
     2521 ttcaaaaaac agcgacgata agaacctgaa tatggcttcg ttgctggaaa gggaggggct
     2581 cggcatcgaa ttgcctaaaa ctggagagat ccgcacggga actattgcaa gtatttcacc
     2641 cggtcagatc ctggtcagtg tcggagcaaa atctgaaggt ctcatcagcg gaaaagaatt
     2701 cgaactgatc ccacccgaag aattcgccag tctggaagtt gggcaagaaa taccggtata
     2761 tgtcatcaca gttgaagact ccaacggcaa tttgctgctt tcatacgtgc gcgctgtgga
     2821 agaatcgagt tgggacaaag cggaagaagt tttcaagaac aaaaagacct ataaaggcaa
     2881 agtcgccggt tacaacaaag gcggcttgct ggttgctttt gaaaaactgc gcggtttcat
     2941 cccctcatcg caactgagct atgagagatt gaaggaagcc gccggcaaca ctcccgaaga
     3001 acggtacggg aaaatgatcg gttcagatat cgaagtcagc gtcatcgaag tggatcgaga
     3061 gcgacaccgc ctcattctct cagaacggct ggcatgcggt gaaacccgcg atacgatccg
     3121 cgaaaaggtc atcagcgacc ttgagatcgg cagcgtgctg accggcaaag tcaccagtgt
     3181 ggctagcttc ggcatttttg tgaacgtcaa tggagccgat ggtctggtgc acgtctctga
     3241 actctcctgg gatgaaatca gcgatccgtc cgagaaattc aaggttggtc aaaccgtcaa
     3301 ggtcaaggtt atcaatatcg atgaagataa aaagcgaatc gggctttcca tccgacagct
     3361 tgaggatgac ccctggttga aaaatgtcga gaatctcaag gtgggcatgc tggttgaagc
     3421 caccatcacc aacctgacca agttcggcgc ttttgcaaag atccagggcg acatcgaagg
     3481 gctggttcac atctccgaga tcagcgacag ccatatctcc catccaaaag aagttttgca
     3541 tgatggagaa aaagtgacct tgcgtatcat caaaatcgaa ccggaatcac accgcatcgg
     3601 gttaagtctg cggcgtgtcg aatctgccgc ctatgcggat atggatatga aattgctcaa
     3661 acaggagttg gaaggcagcg atatccatgt ttccggtggc gatgatgaaa agacaaattc
     3721 tgaagatgct gaaggctccg atgccacccc tgaaggggaa tagtttcacc ttgttttgtc
     3781 atctcaagac gaggagcgcg tttgccacgc gctctttcaa ttacggttat aattaacctg
     3841 ctttagatcg aatatgccgc aaaacgaacc tgtcttagtt gaaaaaccat accaggtctc
     3901 tgacgaaagt tttataaaac gtctccgcaa ttttcttgtt ctcactttcg ttggattcct
     3961 tatttttctt ctgggcattc gccctgatct tttccacatg gatcgcagtc ccgtcaccgg
     4021 cttcatacag atctcggtta gtgtggtcgg gttagggatt ctctgcctgg gaggctatct
     4081 ctgcatgcac tggctctggc gcggagaacg tccgagcatc gccgccgaga tcggtgtgcg
     4141 gttcatcagc accggactgg tgatcgccgt cttttcagcc gtggctgatt ttttcggttt
     4201 cggcagccat ccctatcctg aatcactccc ttatctggga gagtggcagg tcttgggaat
     4261 cgagatcggc gaatatacga tcgcactggg atttattttg atgatcccct tccccaaaat
     4321 ccaattattc cctcgccttc cccaatcaga caagcccaac cgccgctagc atttcttgat
     4381 tggcagcaaa tttgtaatca gccaatcctt tcttttgagc aatctccgct tctagcgcat
     4441 acaattggtc aaccatattc ttattcacaa ccgtcttctt cgaatcaaga attttagatt
     4501 ccagaacctg ccaatcagga cagtctatat cctgctcttg cagaaattca tatgcagcgt
     4561 gcgcgccctt catcccatct tttccgctaa tacccaccaa cccataactg gcgagtcgag
     4621 accagccagc cagaaagatg ccggatatgt tcttttcaag aacaggatcc atcacctcgt
     4681 atgaaattcc atcctgcggg taatcaggat aaggactcac cgtgaacaga tcatcggata
     4741 caggcagtcc catttcatca tccacgctag aacccaccgc cagaatggcg gtatcgacat
     4801 caaagagcgc atgcagagag gtgggcaccg ttttcatgac gtggttttct tcaaccagtg
     4861 tgttttccac gcatttcacc tcctgcaagc cttcgttttc attccccagg aattgaacag
     4921 gtgatttgag aaaacgtaga cgtaaattgg cggaagacgt tttttcatca cagttttcgc
     4981 aagctttttc gataaaagcc caggtcgcat tgggatcctg acccagtttc aacatgaggg
     5041 atgtcacacg ttccatctcc gcttcaaaat cggcacggtc caggttttga gcaatatgag
     5101 cgaactctgt ttcgtgaaac ttgatttcag caggccctct gcgagctatc accagaattt
     5161 cctccacatc acccttttgc agtaagtaac gcgcaacgtc tgccatgaca tttcccaccc
     5221 ccacgatcaa cacctttttg ccgataggat aatcgatctg cgaatatggc ggcagatcat
     5281 tatattttga cacgatctcc cacgcatgat atacccgcgg taagaattct ccctccacct
     5341 tcaattccct gagcttttgt gctccagccg ctattaatat tccctgaaaa cccattgcgc
     5401 gaagttgggc aagagaaaga tccttattct cgccgatctc catattgcca aaataggtga
     5461 tatttttatg gctgagaaaa ctcataaatt gtcggcgcag tccctttttg agtgaagttt
     5521 tctccggata gatgccatat tccgccaacc ccccagggcg tacatcgcga ttgaacaaag
     5581 ccactttcac atctttctca gccaacgttt tcgccgcgaa caagccggct ggtcctgccc
     5641 caatcacagc aacggtcttc tgattcatac gcacctcagt ataatttgta ttaacattga
     5701 ctcataaaat gagtgcgtta atctttttat tttatactac tggaacaaaa ttgaggtaat
     5761 cgttcatgca aaatgatatc agttcttttc aactttccaa tgggttgcgg gtgctgctca
     5821 aggagattca taccgtaccc atcgtcagcc aatggacatg gtacagggtt ggatcgcgca
     5881 atgaaaaagc tggcatacgc ggcatttcac actgggtgga acacctgcaa ttcaagggga
     5941 cagagaaatt tcccggagaa aaactagacc aggtcatttc gcgcgtgggg gggatatgga
     6001 atgccatgac ccacctggac tggacaacgt attatgaaac cctccccgct aaccagattg
     6061 atttggcgct cgatctcgaa gcagaccgca tgcagcatag cgttttcgac gaagacgaga
     6121 tcaatgcaga aagaactgtg gttatttcag agttggaagg aaatgaaaat gatccgctct
     6181 ttcgtctcaa ctcagccatt cagtcgaaag catttcagtt ccatccttat agccacgatg
     6241 tgatcggcac gcttgaagac ctgcagcaca tgacccgcga tcaattattc agatattatc
     6301 aagaccacta caatcccgac aatgccattt tgtgcatcgc gggggatttc aacataaccg
     6361 aagtgaccgc taagatcgaa cgatattttt caagcattcc cccacggtcg atcagcgaag
     6421 aaaacatccc cgtggaacct gaccagacaa atgagaatta tcttgagatc aaaggacccg
     6481 gtgatgcgct ctacatccag atcgcctacc acgcctcgca agccaaccac cgtgattttt
     6541 tcaccctctc cgtgcttgac agtctgctgg cgggtccttc tcctctggtc atgtttgggg
     6601 gtggcggaat ttccaaccga accagccgtt tgtatcaggc attggttgag aaggatattg
     6661 tggtcagtgt gagcggtgat gtgcaggcaa ccatcgatcc ttttttacac accttcatcc
     6721 ttactttgaa cccaaccgct gatccgcaca aggctttgca ggcatttgat aaagaaatcg
     6781 aaaaaatcaa acaggaaaaa ataagccggc aagatattga ccgcgccatc caacaagctg
     6841 aagccatgtt tgcatacggc agtgaaaaca tcaccaatca ggctttttgg atgggctaca
     6901 gcagtatctt tgccagttat gactggtatg agaactatat tcagaatttg cgctcgataa
     6961 ccgatacgga tattcaaacc tttgcacaga aacatttcgc cttccataac aggaccattg
     7021 gcatttattt cccaacacaa gaagaagatc atgatgcaat ccaataatac caacttccaa
     7081 aaccactttc ccaatgcaag caccattcgg cgcttccaac taggcaacgg tatcaccgtc
     7141 ctcgcataca gcaacatgca cagcaaatcg cttaccgtca tcggcatgct tgcgacggga
     7201 tcagcctggg accaagcggg gaaacttgga accgctcatt tcaccgcttc catgctgatg
     7261 cgcggaaatg aaaagatgac cttccgcgag atcagtgaca ggcttgaatc ggtgggtgcc
     7321 agcttaagtt tcagtgccgg aactttgaac acttggtttc aaggtcgctc attggtgaaa
     7381 gattttccca tgctgcttgc gctgctctac gataacctga ccagcccgat ctttccccag
     7441 gaatacatca cccgcttgcg caatcaactg atcacttcac tgttgatccg tgatcaagac
     7501 acgcaagaga gggcagccct ggagtttgat aaagtgctgt tcaaaagcca tccctacgct
     7561 cttcccgtgg atggatatgc cgaaaccatt gcaaacatac agcgccacga tctcaccaaa
     7621 catcacagca tgtatcatcc tcacaatgcc gtgatcgccg tttcgggcgc tctcccgctt
     7681 caagacatcg aaagcatgat caaagggcag tttgaccaat gggctccaaa gaaagaaagc
     7741 gtaaaccctg tttctaccgg ctttgaagcc atcatcgaag aaacccgcag ccatatcccg
     7801 atcgaaggga aaagtcaaag cgacatacaa atgggcactt ttggaccctc cagggcgtca
     7861 gcagattact atgcggctta tttgggaaat cacgttttgg gtcaatttgg tttgtacggc
     7921 aggatcggca ggtctgttcg caacaaagct ggtctggcat actatgcgtt cagcagtctt
     7981 aatgccttgc cagaaatcgg aagctgggaa tttaacgctg gtgtaaatcc agtcaatgta
     8041 gaaaaaacca tcacattgat caaggacgag attcgacatt accttgaaga acctctccaa
     8101 gaggacgagc ttgaagattc aaaatcccat ctgatcggga gattgcccat gtctctggaa
     8161 tcaaacgccg gtattgccaa cgcattgctc tctatagagc gttttcatct cgggctcgac
     8221 tatttccaaa actatgccag cctgatatcc agcatcacgc ccgctcaaat tttagaagct
     8281 tccagaaggt acctggatcc ggaaaaattg attatcatta gcgcaggagc tgagaaatga
     8341 tcttgcgcag cggggtcgac atcattgaaa taacacggtt cgaagaacaa agaccagaaa
     8401 ttcgcaaacg ctttcttgcc agggtattta ccgttcgcga actttccctc atccgaaatt
     8461 cgcacatttc ggcagccgga agattcgcag ccaaagaagc ggttgccaaa gcattaggtt
     8521 gcggcattgg taaagtccgt tggcaagata ttgagatctt acagggaaaa gatggggaac
     8581 cattgctgat attgcacgca aaagcggaag aaataagcca acaagcggga ctgaaaacat
     8641 ggtcgctgag catttcccat tccagacaat atgcaatcgc gtttgcggta gccgtcggca
     8701 catcttgata ggacacaata atgactgaaa agattctgat tgtaagcgat aagatttcag
     8761 aacaattgta tgatccatcc aagcaaaaag cactcaaagg aatccgcttt atcgtcagtt
     8821 ccggcgacct gccgtattat tttttagaga atctggtcag cacatataat gttccgcttt
     8881 actatgtgcg cggcaaccat gccagctttt acgagagcga aacaggcgtc attcacgacc
     8941 atccttgggg ctgcattgac ctgcatgtgc gatcagttaa ggatccttca ggcttcttgc
     9001 tggcaggtgt gcagggcagc catatgtaca ataacggacc ttatcaatat tctcaggcgg
     9061 gcatgtggca gttcgtcttt agacttatcc cttctttgat ggctaatttt gtaatttctg
     9121 gcagatacct ggatgtattc atcacccatg ctcccccctg gggcattcat gatgggcaag
     9181 atatcgctca tcagggcatc aaagcttttc tgtgggtgtt aaaagtgttc aagccactgt
     9241 accattttca cgggcatgta tatgatttcg gcaaaaactc acccatgatc agccaatatg
     9301 aaaacacgca agtcgtgaac acgtacggct acagggttca tgagatctca atgaacaaaa
     9361 aacccgcgac aaacgactag aggaagcgat gagttttgat tcattcaagc accaatcgaa
     9421 agcagatttt gatgaggcgt atcttcacgg attcatccgt tccattgaaa actggctgcg
     9481 gttcgaaaaa acgcaactcc tggcattcaa tgagatcctg aaacgtattt cggtgcagag
     9541 ccaatacgat gcgggcgttc aggagataga gatcgatgcg atcgttggaa gcgtgaaccg
     9601 ctacgcggat ttcgacaatg cctttctccc ccgccaaaag catacccgtt cgcgttggga
     9661 gaagattgac cgggcttacc tgcgcgaaga aatattaccg ccggtcgacg tataccaggt
     9721 cggaaacttc tattttgtga tcgatggtaa tcatcgcgtt tctgtggcaa agcagcgcgg
     9781 gcaaaagtgg atcgacgcgc acgtcgtaaa gatcgagact ccctacgaag ttgacaggtc
     9841 ctttgatttc aaccaactga ttctgaaaga agaacagaat cagttttacg aaaaaaccaa
     9901 cattaagaaa tcgtatccag acgcgaacat tgaaatgagc atagccggtc tcttcgagcg
     9961 tctgcaagaa cacattgatg ctcatcgtta ttttaccggc gaattcttac agcgcgagat
    10021 cagctacgaa gaagcggtta gcagttggta cgaaaacatc tatttgccgc tggtaaagat
    10081 catcaggcag cagaaaattc tcgaccaatt ccccgatcgc accgaagcgg atctgtattt
    10141 ctggattatt gaacatttgg cttttttaaa agctgagtat cacaaagatt tcagtttcga
    10201 agaagccgct ggcgatttct tgatgaagtt tcatcccggc ttcattaaat taacagtagc
    10261 caaagcaaag cactggttca agaagaagcg ccgctaacgg tcttcttcca gtaaaaagtg
    10321 attggggaga atatattggc tgacttcaat gcgcaaacct gccagctttt tcgatatcgc
    10381 acctgccgta acggtcggat ttacagtgca acgatcaggt ttgccaccat atttctgctg
    10441 gtaaaacaaa actgccgcat tgatcttttg ttcaatggaa atatccttgc ggtcatcgaa
    10501 ccatagcatt cctttttcca tataacctca gacacattat agaacaaatg ttctttcccg
    10561 tcaagcaatt ttctccacaa gcccctttaa tggtattttt acttttcttt ttttgaggta
    10621 tcctttaaca ttaaaggaat tcgtccatgg ttcatgcaag gaggatacga tgataaacga
    10681 agcaattcag gatgcagaaa aaagaatgaa aagtgctgtt cagacgctag aagaagatct
    10741 ttcaggaatt cgcaccgggc gcgccagccc ggcattgatc gaacgtttat ccatagaata
    10801 ttacggctca cccgtcccgt taatgcagtt ggcaaccatc agtgttcctg aaccgcgcca
    10861 gttgctcatc aaacctttcg atgccacatc catcaaagac atcgaaaaag ccattctcgc
    10921 ttctgacctt gggctgaccc ctctgaatga tgggaaaatc atccgcctaa acatcccgcc
    10981 cctcaacgaa caacgtcggg tagaactgac caaactggtc ggaaatcgtt gcgaagattc
    11041 gcggattgcc atccgtaacg ttcgtagaga tatcatcaaa gacttgcgcg actttgaaaa
    11101 agaaaagctg atctctgaag atgatctgaa acgtggggag gaggaactgc agaagcttac
    11161 cgataaaatg atcagcgagg tcgattcgat tggtgagcaa aaagaaaaag agatcatgga
    11221 ggtttaacca acctgcatgg caaaacaaaa agagctgaca ttcgagaagg ttccacggca
    11281 cattgccatc atcatggatg gcaatggtcg ctgggcaaaa gcccgcatgt tgcccagaat
    11341 ggcggggcat cgcgccgggg ccgagaattt gcgtcgcatc atcaccgcct gcgtggattt
    11401 tggagttcga tatctgacga tcttcgcttt ttcaacagaa aactggaaac gcccggctga
    11461 tgaagtgaac ggtttgatgg agatctttgc cgaccttctt gaaaaggaag tgccaaaact
    11521 ccacgctcaa ggtgttcaaa tccgccatct tgggagtata caggagttga acccgacttt
    11581 gcaaaggaaa ctgcagaacg caattcaaac cacgcaaaag aacgaacgac tgatcctctc
    11641 catcgcttta aattatgggg ggcgagatga gatcattcac gccgtaaaag agatcgttaa
    11701 aaagggtact ccagcagatg ccatcgatga gcagaccatc aaagatcacc tgtataccag
    11761 gggtatgcct gatcccgacc tggtgatcag gacatcgggg gaacttcgga cgtcgaattt
    11821 tctcctctgg caaactgcct attccgaatg gttgttcact gaaacatact ggcccgattt
    11881 caacaaagat cacttacaaa aagccatcct cgaatatggc acacgttcaa gacgattcgg
    11941 gggaagaaca aaaaaagagg aagaagaaac atacaatggc taaacggatt ttgggtattg
    12001 cgatcatcgc gccaattggc gtggggttgg tgatcgcggg gggatggatt tttaccttgg
    12061 gagcatccgc aatcctgggg atagctgcct gggaatattg gaattttttc caagaaagca
    12121 aatttgcccc ccacaaatac tttttaattg tagctaccgt gatcagtgcg gtcgctcgtt
    12181 atctttttaa tggcgcatat tttgaagcag tgttcgcctt attattcatc gccagcatca
    12241 tcatcagctt aatccgttac gagaaggacg acgcgaatag ccttgcgaca atgggattgg
    12301 aatggattgg tttgatcttc attgcttact tcggttctta cctcatttca ctgcgcttct
    12361 taccggatgg aaagatgtgg atcttgttaa ccgttcctgc cattggatgt ggagatattg
    12421 gtgcgttttt aatcggcacc tattttggca agcataaaat ggctcccagg gtaagtcctc
    12481 ataaatccat cgagggatat tttgggggaa ttcttttcac ggttctctac gcgttgcttt
    12541 tttctctgat cttcaccttt ggagctgcca acatcaccgt tggtcgttcc atcatgcttg
    12601 gtttgatact gggcatcatc agccctttgg gcgatttgtt ggcaagttta ttgaaacgat
    12661 cattcaataa aaaagattct gggaagatca ttccggggca cgggggaatt ctggatcgcg
    12721 tggatacctg gctgctggga gcagcggctg cttatttcat catcaggttc ttttggatat
    12781 aaaaaagctg cttaaaaatg aactgcacct cttcgtgttg gttgtgtcca accctggaga
    12841 tgcagttcaa aaaagcagtt cttttgtatc agtcgatcgc gtattggatg gtcgcgcttg
    12901 aagtgatcac ataattacct gctgagatcg ggaccgtggc cgctgattgg gaatatccac
    12961 cgccccccat gcccatgctg taagaatcgg taacgagata agggacatcg ctggaataca
    13021 ttgaaatggt caacacctcg cccaatttta agccagatga ttcagccact tcttcagcct
    13081 gctggcgggc aagttcgatc gccagttcgc gcgactgggt caaagcagcg cttttatcct
    13141 gcacgtcaaa gctgattcca taaatagaat ttgctcccga ttccgttatt ttcccaagca
    13201 ggtcacccat gctgctcaga tcgcgaacgg tgacgtagac actgttgttg accacaaagg
    13261 tggtgccggt aatgttccca ttgtagtcat aattggactg cggataaacg ctgaaactgc
    13321 ttgtttgaat atcttcttcc gcgatgccga gggcgaccaa ttcatctttt attgtctgtg
    13381 cctggtttga attctcggtt atggcatccg ttacattatc attttgtgtc gtgataccaa
    13441 cgttgatata agcgacatcc gggacaacct tcacaacgcc tgttccggtt accgtgatgc
    13501 ttttctgaac cggcgtgtta gcggctgatg ctgaaggtgt tccactgcaa gctgccaaaa
    13561 gaaccaccat cagcgctgat aaacctgcaa ttaaataatg ttttttcatt ctgcctccat
    13621 taaggtatat cttttaaacg ccgtgccttc caaaaagttc ccagagtatc ttaataatcg
    13681 ccagaatgct tgcacggtaa gttgtatcat gttatcatga aggtgccttt ccagccggtt
    13741 gcccccctca atcggttggg aaggtcttct ttttattttt tcttcccata tgtttttaca
    13801 agcacaaaaa taccacctgc aattagcaac cctgcccaca ccagggtaga attccaatat
    13861 tgaccccaat cccaacccag cgcccgtgcc agcgtcgaaa gtcccactcc aatcaagaca
    13921 atgccaccga aagtgagcaa ctgcgcagac ggattctgga acaggtcacg cagatcatct
    13981 ccaataagac gaaaacgcgc acccaaatca ttgagctgga aaccagcgcc ttcgtactgc
    14041 tgcggtggaa tgatcagcca cagcaaaaga tacacaaaca ctccccattc gccgacgatc
    14101 aaccataaaa taagaaagat gcgtacaaaa atggggtcta tctggaaata ctctgccaaa
    14161 ccaccgcata cacccccgat gattttgttt tgcgaactgc gatacaatct atctttcatt
    14221 gctacctcca aaaatcttat tgtatttcaa ttgaaccaac accggactcg atttcaatat
    14281 cgacgcggtt cgcggcattg ggatactcta ctgattggta gacatcacca ttccggtgga
    14341 accgtgccgt atcaatggtc ttccctatca aacctccgac tttgattgtc gcggcaacat
    14401 tttgagggac gtggatcttg atactcgagg ctcctgactc tatgtgaact tttgtttcac
    14461 ctgctttttc tggcagaaag atctcggttg aagaagcgcc cgtttccaaa cgcaattctt
    14521 tcagttgtgc atttctcaga tccaaatgat tgtcgcttgc gccggtttcc atttttatct
    14581 tgagcgggag cgtttgattt accacaaggt cccacatcaa cctctgctct ttttgaaagc
    14641 gcggtaaaag attcatgaac gccaccttgc tttccagttt gatcgtgctt cttctatcgg
    14701 cagaagaaac cttcgcattc acgccaccga tgaagtttcc ctttagtatg gctgaaggga
    14761 gatctgcagc ggcgagggta atcgttcctg ctccatgttc cattttgatg ctcacctgat
    14821 cgacttctgt caatgggact tccaactcat ctttgactac ttccatgcct tgggaaagta
    14881 ctggaaccaa caaaatccaa ctgcccgtta ctatcaaagc gaagggccat atcaagctcc
    14941 atatagaaaa cccgaattta tatgaaaaga gaaaaataac tcctaatatg atcagaacag
    15001 caccccagaa aattcgattg cgcatggttt cctccgtttg attctattta tatacgcttt
    15061 ataaaaaatt aggtttccag ttgtttactg cttgacaaga gttatgaaac agaatactat
    15121 ttttatgata aatctaatgt aaattggaag gaacgtcgat gcatcatact tgcttgtgta
    15181 aaatgcgggg ctcaccatag gactgctctg cgacaaaggc actcatggaa gccatccggt
    15241 cgtagtggca ggagccccgt tttcacttat agatcgatgg cttttttctt ttaatgtcct
    15301 tttatcgaaa tgacgggagc atgcaaatta tcaatactat taccacatta atgggaaaca
    15361 tcccgctggc atggcttaac aaaatcagcc cttcacattc taccaggatt gcagcgaatt
    15421 tggagcattt ctatccatct cacaatggaa aagaccacag cggtttcaag acgaccccag
    15481 gggcagctat ttctgcaagc ttgcaggttg cccgccgttc tgagaagcag gacgagttga
    15541 gcatgggtat tgtgccatcg tttgatgagc atcgccttgg cgccaacccc tactcacagg
    15601 atggtgactg atggcaggta tcttctcaga aatgaaaaat gacatccgcg tggctttgga
    15661 gcgggacccg gcagcgcgca actgggtgga agtggctctg tgctatgccg gggtacatgc
    15721 tatttggaat caccgcatcg cccactggtt atggaagcga aatctgaaat tgctggcgcg
    15781 ctgggcatcc caattgagcc gttttttcac cggcatcgag attcatcccg ctgcgcagat
    15841 tgggagtggg ctctttatcg accacggcat gagcctggtg attggcgaaa cttccatagt
    15901 cggaaaaaat gtgaccatgt accacaatgt tacactgggt ggggtcagcc tgcagaaagg
    15961 gaaacgacac ccgaccatcg gcgacgatgt ggtcatcggg gcgggcgctc aggtgctggg
    16021 tgccatcacc gtcggcaact gcagccgcat cggagcgaat gcggtggtag tgaaagatgt
    16081 tccccccgat tccgttgtgg taggtgtgcc agggcgcatc gttcagaaag agcacgttga
    16141 aatgcacatt cctgaccttt cgcacaacca gcttccggat gtgatcgccc agcaattatt
    16201 gaatctcgcg caaagattgg aaaaaatcga aaacgggtta aaagacaaag ggtaaacgca
    16261 aaaaagagtg catcatcctt tcttgcgcaa catcgtatag atcaaataca ctaggcaacc
    16321 accacagatc acgatcagga tgatcccaag aatctctctt tggttcgaag tcatcgcttt
    16381 ttcttgcagg gaatcgcccg tttgcgaatt caacgcctgg ggggtttgcg gtggaatggg
    16441 agtgcgagtg ggaggcaagg gtgtgttggt ggggatgagc gtgggggtag aagtaaaagc
    16501 cgggcgaacg atcaaatttt gcccttcata aatcgctgca ttatccgata aattgttcaa
    16561 gattcggatc tgatcgacgg tcacaccgta tgtgatcgca atgctccata aagcttgccc
    16621 tttttccacg acatgggtta tggagccatc aggtttcggt gtagaaacaa ccaccggcac
    16681 cacacccggg gtctctgtag ttttggctgt caatgttgaa ctggtaggtg caatcgaata
    16741 atctacggaa atattgagaa cataatagac aatgttatcc acctgcgcca tacccgctcc
    16801 gatctgggag gcgtactgat tcagcatgac accagcatta ttcgaatcac cccaggtcga
    16861 attcaacaat gtttcaagag acgtgctggc atccaccccc gcccaggctt cccgaacctg
    16921 caccccggtg atgattgtgt aaccaatttg ttttgcccgt tcggtagcat cgctacctcc
    16981 ttctcccaca tgcccttgcg actcaccagg aggggttgtt ccataggtat tagccagata
    17041 cgtggcatgt tgttgggcag ccgccatcaa atagacattg gttgccagtt gattcaaact
    17101 gttgagattg cggtaggtat tcaccgcaac caccagatca taagcgctga ttgcatcagc
    17161 ttctacaaaa gaaattggaa taagcataca acttatgaac aacaagacaa gaaggagagc
    17221 ttttttcttc ataaaaggca ttatacccaa cattccggca tcttattgtg acgatcgttg
    17281 ccccaaaaag attaacgcga atccgataat ggcaaccacg ataatcccca tgatcaccca
    17341 ttgatcgctg aggggttggc gattttctct ggtagatggt tgtgcagcaa gtagcggtgc
    17401 ggttgtggtc acaacgggaa actggttttc aaggtggatt cctatcgtaa tggtaggtac
    17461 caacgaggga gtgggaacca ttttgatcag cagtttttga ccggtataaa tcatgctttc
    17521 agaggtaaga ccattcaact gacagatatg gtcgatcgtc acaccatagg ttgtggcaat
    17581 gctccacagg gtttgaccct gcaacacctg atggatcact tttccactct cgtcaggttc
    17641 agccatctcc accggtgcta tatattgcga gatagacgcc cccgtcgaat ttccgccaga
    17701 aatgcccggg tatgcgccag aggtgggttg gacggtcaac ccggcgtcgc cccagaaagc
    17761 agcaacattc agcacataaa caacgttatc ccccgaaacc gccacgccgg ctcccacgtg
    17821 ttgccctctg gtatgaagca tggtatgggt atgctgtgaa tccccccagg ctgtgaaaag
    17881 taaaacatcg agatctgcag aagcgggcag gttagcccag ttttcgttaa tgtccaagcc
    17941 gggaacttgc ggataaccca gtgctgccgc acgtgcatcg gcgtcggtac caccaactcc
    18001 cacgtgcccg gtgagagccc cgctttgcga agccagatat tccgcgtgcg tttgggcgga
    18061 gatcatcagc agagaatcga tgaccagggg tgcaagaccg ttggctgcac gatacgcatt
    18121 caccgcgtcg atcaattcat acgcagtagg aatcagcaag ggtgctgcat gaaccggtct
    18181 cgtaacagcc agacacatga tgagaaaaac gagactgact acaatccagt tctttttcat
    18241 tccatcaaga ttctatagca tcagtttcca atattccaat ctcgaaaata ctccatacga
    18301 tcatatgcgg tcaggtccaa tgagatcggc gtgatggata cgtaaccggc gctcaccgcc
    18361 ccaatgtcag tcccttcttc cggaacgccg gtcggggcat ccccgccgat ccaataataa
    18421 ggacgaccgc gcggatcttc acggcggacc aatgcatcgc gatacacacg taacccctgt
    18481 cgggtaatgc gatatccctt gatctcttct aatggaagat agggcacatt gatgttcagc
    18541 agcacattct tgggcaactt gttttcgagc acgcgtccgg ctaccatgcc tgccacctgt
    18601 gccgcagccc gataatccag aggaccgccg tggtattccg ccgcatccaa agaaacagcg
    18661 atgctgggaa tattgctaat ggtgccttct atggctgcgg taaccgtgcc cgaataggta
    18721 acatcatatc caaggttggc attggggttg attcctgaca caacaaagtc gaccttttca
    18781 cccaacaaac ccaaaatgat caatgcgaca caatcggatg gcgctccatc cgatgtaagt
    18841 gcctgggtgc catcatcgag caaagtttcc ttcacccgta aagggcggtt cattgttttc
    18901 acgtgccctg atgccgacca attcttatcg ggtgccagaa cagtcacttt tccaagtttc
    18961 cgaatttcct gagcaagagc cagaagcccg ggtgccgtca cgccatcgtc attggttact
    19021 aagaaatgca tgctccattc cttcctcaat cgatccctaa ttataaagcg tgaagcgcga
    19081 atgatggata atcgaaaact atggtatact tttgcggtca ccgaaaaggt ggcaaaaaat
    19141 acacacgctc ttcgatagac tttgcgtgct gagagaaatc agtggtctat aaaatggagc
    19201 ggaggataaa cgcaaaggag ttactcaatg agtgcaaaca tttctatgaa aagtctgctg
    19261 gaaagtggtg ttcactttgg gcatcggacc aacaaatggc atcccttgat gaagccatac
    19321 atcttcaccg aacgcaatgg gattcacatc atcgatctgc agcaaaccgt taaatatctg
    19381 gacgaggctt atgatgtcat ccgcgacaca gttgcggcag gcggtacggt cctttttgtc
    19441 ggaacgaaac gccaggcgca ggaaaccatt caggaagaag ccgaacgctg cggcatgccc
    19501 tatgtggtcc aacgctggct gcctggaacc ttgaccaact ggatgaccat ttaccagcgc
    19561 atccttgaac tggacaacct tgaaaacaag cgccaatccg gtcaattcga caaattggtt
    19621 aaaaaagaaa tcttgatgat cgaccgccgc atcaaacggc ttttgaaaaa tctgagcggt
    19681 attcgccgcc tgaagaacct gcctgatatg ctgtttgtag tggatgtgat gcgcgaaagc
    19741 accgctgtgc atgaggcgaa tcttaagggc attccggtga tatccctggt agataccaac
    19801 tgcgatccca acggcgttga ttacctcatt cccgccaacg atgatgccat ccgcgccatt
    19861 aaactacttg tcggcaagat cgcggatgca gtgctagaag gtcaagacat gcgcaaagaa
    19921 gaagttgaac tcgaagaagc ctctgtcatt gaaggtgctc caagcgccga agcagtccgc
    19981 cgacaggttg ctttggaaga agaaatttcg gatgaagagc tgctgggtgc atcgacgcta
    20041 gccaaactta ccgtcaataa agacgacaat ccggatgatg agtatctcga cgatgatgga
    20101 gatgaagagg aagaaaagaa ttaggtctga agaaaaggat ttatacgatg gaaattacca
    20161 ctgaaatgat caaacaactc cgcgaagaaa gcggcgctgg tatcttggat tgtcgcaaag
    20221 ctctggtaga taccaatggc gattttgaga aagccaaagt cattctgcgt gaaaaagggt
    20281 tagccactgc cagcaaacga gcagatcgtg tagcatcaga aggcatggtg gaactctatt
    20341 ctcacggtgg cggaagagtg ggcgttatgg ttgaagtgaa ctgtgaaacc gatttcgtcg
    20401 cacgctctga agacttccgc acttttgccc acgaaattgc cctgcaaatt gccggcgcat
    20461 cccctctgta tgtcagcgat gcggatattc ctgaagaaac gatcaaagat ctcgaaacaa
    20521 gagcgactga aaaggcaaaa caagaaggaa aacctgatac aataattgca aagattgtgg
    20581 aagggtcatt gaaaaaatat aaagacgaaa acgttctgct acggcagcaa tatattcgag
    20641 atgattcact ggtgatccaa gacctcctaa atcagaccgt tgcgaaaacc ggggaaaaca
    20701 tcatcatccg ccgctttgcc cgttatacac tgggtgaaac cagccaaaac gaagaggaag
    20761 ggaattaaat taaaaagcca accgcacggt tggctttttt ttagcaagga gtgaaatggc
    20821 agacgtaaaa tatcatcgtg tggtaatcaa gctgagtggt gaagcgctgg cagaagatga
    20881 cgggaatggg atagatccca agcgtgcagc caatattgcc aaacgcgtca gcgatatcca
    20941 tgccatgggg gtggatgtca gcatggtaat cggtgccggc aacctgtggc gcgggcgcga
    21001 agggcttaaa tggggcatgg acagagctac cgcagattac atgggcatgc tggctaccgt
    21061 catgaacgct ctggcattaa tggatgccct cgagcgcatt ggcgttccca cccgtgtgca
    21121 atcagccatc gaaatgcgcg cggtagccga accctatatc cgccggcgcg ccatccgcca
    21181 tttggaaaaa gaccgggtgg tggtctttgg cggaggaaca ggcaatccat ttttctcgac
    21241 cgataccgct gctgccttgc gggctgctga aatcggcgca gatgtgctca taaaagccac
    21301 caaggttgat ggcgtgtacg acagcgatcc tcaacaaaac catggagcga aaaagtttga
    21361 acgcttgagt tacatcgagg ctctgaacct gcggcttgaa gtgatggaca gcactgcttt
    21421 gaccctgtgc atggaaaaca aattgcccat cctggtgctg aacctttggg atgacgaagc
    21481 gctgctcaaa gccattcagg gagaaaacgt cggcacgatg gtctgtgatt agtaccacgt
    21541 ttgttcttca aacaggcgga agggctacta cccaggtagc ccttttttgt ttgcttgact
    21601 ttttcaccaa tccatagata atattcaaaa ttcatacaat gcggtgacgg agaaaagtag
    21661 ttcttgcaga accgacaggg agaccggggc acagactgaa accccgatcg ggcaataaga
    21721 gaatgaagtt ctctcctgag cagtttgggt gaaagatatg cagtagtcca aaccgcaggt
    21781 gcagcgatac aggcatagcc aatcagcaac agaaggttgg tgaaagagtg ggctgaattt
    21841 cagccaaaca gggtggtacc gcggattccc ccgccccttt gggcggggtt tattttttaa
    21901 catggagtca ttgtgattat ggaagaacaa aaggatctgg tcgtcatact gcaacaaata
    21961 caacaaaaag cgctggaatc actacagcaa attaaagacg cggatagcct ggagcactgg
    22021 aaatccacct tcctgggtcg ctcttcagag gttatgcggg ttttcaaaga gattccttct
    22081 atgccaaccg aagtgagacc agcgttgggg caaacagcaa atgtcgtaaa aacagaactc
    22141 gagaagcagt ttgaaacggc gcgcgaacgc atccagcagg ctgccctggc gaacaatctc
    22201 ctggctcaaa agttggatgt gacgctcccc gggcgaccgc cggaacgcgg gcgattgcac
    22261 attgccacac aaaccttaca ggagatctac cgtatactcg cggaaatggg tttccaggtc
    22321 tatcgctcgc gcgaggtgga atcggatgaa tataacttcg agttgcttaa cattcccgct
    22381 catcatcctg cccgcgatct atgggacaca ttctatacta aaaaggaagg ggtgatcctg
    22441 cgcacgcaca cctcgccggg gcagatccat gccatgcgcg aatatgcacc cgatccggtg
    22501 cgtatcattc tgcctgggat ggtctatcgc tatgagcaga tggatgccag ccatgagatc
    22561 cagttcaacc agatcgaaat gctggcggtc ggcaggaaca tcacctttgg caatctgcgc
    22621 ggaacgctgg aagaattcat caagcgcatg ttcggtagtg gggtggagac gcgcctgcgc
    22681 ccatcctatt tcccgttcac cgagcccagc gcggaaatgg atatgaagtg cattgcctgc
    22741 aatggcaaag gttgcggggt ttgctcggga accggctggc tggaaattct cggctgcggc
    22801 atggtgcacc cggtggtatt gaagaatggc gggtacaacc cgcaagaatt ctcaggtttt
    22861 gcagccggtc tgggcaccga acgcatttct atgttgcgtc acggcatgga caatatccgc
    22921 aatttctggg caaatgacat tcgcttttta agacagttct aaagaggatg agaaaatgaa
    22981 gctggtttta tcgtggttga aagaattcgt tgacattgat tgttcgttgg aggagttagc
    23041 ccgcaccatg accatgatcg ggttagaagt ggagaacatc caactggtcg ggctcccgat
    23101 accccaacaa gaacacctgg aacatgtgta cagcgggttg acatgggaga aagacaagat
    23161 agtggttgcc gaggtaagcg aggtcatgcc ccaccccaat gctgaccgct tggtgctctg
    23221 caagctcaat gatgggcaag gggagcaaat tgtgctcacc ggagcgccca atttattcga
    23281 ttataagggc aagggaccac tcagcagacc cctcaaagta gcctatgcca aagaaggttc
    23341 aactttgtat gatggtcacc aacccggaaa agtgctcacc actttgaaac gcatgaagat
    23401 tcggggggtg gaatcgtcat ccatgatctg ttccgaaaaa gaactgggca tctccgatga
    23461 acatgaaggg gtgatcattc tggatgacga tgctccggtg ggcatgccat tggttgagta
    23521 tatgggcgat gcggtgatcg aggtatccat tctgcccaac atggtgcgtg atgcctgcat
    23581 gctgggagca gcgcgcgaga tcgcagcagc aacccataaa cccctgcgcc atccgcagcc
    23641 caaactgcaa ggagacggag ccaagatcga gggtgaagtg aagatcgaga tcagcgatcc
    23701 cgagttgaat ccgcgcttcg tcttcggcat ggtgcgcaac gccaccgccc aacccagccc
    23761 atactgggtg cagagaagat tgcggctggc gggcatgcgc cccatcaaca gcaccgtgga
    23821 tgccaccaac tacgtcatgc tcgaagtggg cgaacccctg cacgccttcg attacgatat
    23881 tctaaaaaaa cgagcgaatg ggcacacccc caccatcatc acccgcgccg caaagcaagg
    23941 ggaacggctg accaccctcg acaacgtgga acggacgctg gacgatttca ccgtcctggt
    24001 cacggatact gccggtccgc ttgccctggc aggcgtgatg ggcggtctcg aaagtgagat
    24061 caccgatgta acacgcacca ttctgctgga aggcgccagt tggaatttca tccacacccg
    24121 gcgcacagtg agcgctcaaa aattgcagag cgaagcaggg taccgtttct cgcgcaacat
    24181 tcacccggcg ctggcaaaat tgggagttga actatgcctg agccgcatcg cggaatggag
    24241 cggtgggcag attgccagcg gactggtaga tgcgtatcca aaaccgcata tcgatccaac
    24301 cattgagatc actccgcaag atctgcaaca cctgattggc atttccttat cccctcagga
    24361 aatagccagc ctgcttacca gtctggaatt cgaatgcagc attgagggcg atcatatcac
    24421 cgttcattgt cctcctcatc gtatggatat cggagaaggg gtcgttggga aagccgacct
    24481 tatggaagag atcacgcgcc tctatgggta tgatcgcatc cctgccagcc gcctggctga
    24541 tgaattacca ccccaacatg cgaacggaca ggatgatttt gaaaaacagg tcaagcacgt
    24601 gctggtcagc gtcggattgc aggaaaccat caattaccgt ctgacctcac ccgaagacga
    24661 agcgcgcatg ctgctcgatc agacagcact ggctggaaaa gaatatgttg aattattgaa
    24721 tcccatctcg caggataagc gcgtcatgcg ttcttctttg attccgcaga tgctggttaa
    24781 ccttgaaaag aatgtccgcc atgctgaccg gctgggtctt tttgaagtcg gaccaatctt
    24841 cctacccaac aaaaacgaag cactacccaa ggaagagtac catctttgtc tgggtttgac
    24901 cggcttggag ccgaagaaaa gctgggatag gaaagattcg cggaagctgg atttttatga
    24961 tctcaaaggg tatgtggaag ctctgttaga agcgctgcac attcagaacg caattttcgc
    25021 ccccgccgga catgctctgt tccaccccgg aaaatgcgcc gtgctgcgct ggaacgatct
    25081 ggaattgggc att
//
LOCUS       DAOSDS010000024        24591 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_907_length_24591_cov_3.837952, whole genome shotgun sequence.
ACCESSION   DAOSDS010000024 DAOSDS010000000
VERSION     DAOSDS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24591
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_907_length_24591_cov_3.837952"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLL88_04525"
     CDS             complement(<1..618)
                     /locus_tag="PLL88_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ00861.1"
                     /translation="MKSLNKAGINLFLGILAVSTASLLIRKAQGTLPSTVIAAGRLIS
                     ATLILAPFGGKRALREFFALPRAQRSPIILAGFFLGIHFLVWIISLEMTSVASSVILV
                     TTTPIWVALFSPLVLKETITRNVMLGLMIAMLGIVVLFLGPQGGAQGGTFDWRSLLGN
                     FLALVGAWMAAAYTMVGRKVRDALPLKSYVFLVNGVAALVAATAAL"
     gene            695..1318
                     /locus_tag="PLL88_04530"
     CDS             695..1318
                     /locus_tag="PLL88_04530"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOJ00862.1"
                     /translation="MSFILPELPYAYDALEPHMDAQTMQIHHDKHHAAYTTNLNAALE
                     KYPEFFEKSIETILSDLNAIPEEIRTAVRNNGGGFYHHNLWWTELAPNAGGEPIGTLA
                     EVIKKDFGNFATFKEQMGAAAMGRFGSGWAWLSMRSDKTLIIHSTPNQDSPLSQGLLP
                     LLGIDVWEHAYYLKYQNRRKDFVDAFWNVVNWDEVERRFAEENSAAS"
     gene            complement(1356..2102)
                     /locus_tag="PLL88_04535"
     CDS             complement(1356..2102)
                     /locus_tag="PLL88_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOJ00863.1"
                     /translation="MAKVLGIDVGGTGIKGALVDTRKGQLLTERKRYPIDGKIKPEEV
                     LDLIATFVNDLQWSGTIGIGFPAIVKNGVALSAANISKKWIGMNINQEVFEKTGLHTA
                     TVNDADAAGIAEMYFGAGRPFQKEIVIVLTLGTGIGSAIFVRGELLPHTELGHLEMRG
                     KIAEKRASDAVRQKKSLHWKEWSILLQEYLEYLELLFSPDVFIIGGGASKYHEKFFPH
                     LRVNAKLLPAEHLNLAGIIGAAWYAEHGKK"
     gene            complement(2111..3235)
                     /gene="mltG"
                     /locus_tag="PLL88_04540"
     CDS             complement(2111..3235)
                     /gene="mltG"
                     /locus_tag="PLL88_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOJ00864.1"
                     /translation="MRKKSVYLWIVLLALILFSTLYLFLFYFPDQTRARFGEADANLN
                     FFQSRRLAVWLYLHGEDLNKAMEADSGEKQEFSILTGETASQVSTNLAQAGFIADESS
                     FLRYLVYKGYDRILQSGTYYFSRTQAPTELALAMIDPTPEDVNFVILPGMRLEEIVQL
                     IPTSGLNFTSEELLSLIETRSGIDIPEIFAGASNLEGLILAGDYTILREMSAAEFLQY
                     LIEQSAAQFTPEVVNAFIRQGLSPYQAMTLASVVEREAVKDEEKPSIASVFLNRLQVN
                     MLLQSDPTVQYALGWDEQTGSWWKTPLTSADLAVFSPYNTYRVNGLPPTPICAVSVDS
                     LIAVAFPADTEYFYFRSACDGSGAHVFAESYEDHQSNACQ"
     gene            complement(3238..3681)
                     /gene="ruvX"
                     /locus_tag="PLL88_04545"
     CDS             complement(3238..3681)
                     /gene="ruvX"
                     /locus_tag="PLL88_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOJ00865.1"
                     /translation="MEKNRSNERLLGVDPGEKRIGISISDPSGTISRPLRVIRHTSYR
                     ESAREILKICFAMDIKKIVIGISRDEKGEATFSGRKAQRLGEAIQGFADVPIEYWDEA
                     DTTRIAQQTRREMGISRKRRRGHLDDVAACILLQSYIDSHFERGE"
     gene            3792..4625
                     /locus_tag="PLL88_04550"
     CDS             3792..4625
                     /locus_tag="PLL88_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00866.1"
                     /translation="MNKKYPFNVRVVLLIGSLLFLLQSCNFPLAQGNSFNKQVATQAA
                     FAFTQTAEQSQMLTANAPTTPTPAISAITPTSDLDDPKAQLGSAAWQDSLTSGNSFGV
                     GNTEEQISGTNATVWVKEGSFNMYRPTASGGYIWYCAYPNIADFYLEATFTVQNSNGT
                     DEYGLFIRKPDFTDNSGYYFIVTGEGKFNLMRWTASGSTLLGEWKTDENIIKGPDATN
                     TLGIWAEGNTIRLYINDHFAGEFNDTLGWASGYFGLFTNARQTAGMLVKMDEITYWNL
                     P"
     gene            4702..6801
                     /locus_tag="PLL88_04555"
     CDS             4702..6801
                     /locus_tag="PLL88_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00867.1"
                     /translation="MDHLHINAQQQATLDAELISHVFLEGPYKSGKTTIGLARLREAA
                     ASADPAHQVLVLTPQRSLARPYRDAILKPDFPAGVLPALTTYAGLARQFIRLHWPQIS
                     LHSKFRTPAQSPTFLSMETAQYFLRQLCQPLLARGYFLEIRIDQARLFSQILDTMNKA
                     ALVGYPLQETAQRLKDAWNGEAIREKHYEQTQECAQAFRDYCLQNNLLDFSLQVEVFR
                     DHMEQDPQLKKEFFRNYSFLIADNLEEDVPVMHDLLLSLTNDIPSMLLIKDSQGGYRS
                     FLGADALSAERLKHICQHTFTFTDQFDLDTAVQAFNVSLSHCILRKDTSDLPSESRNA
                     FTLCNAQFYPQMITDVCHTIHQLIQAGKADPADIAVLSPYLPDSLKFALSQKLAQAGI
                     PFLSSRPSRSLAEEPVTRAVLAFAKIAHPQWNMGISVEELRHTLMTVFPTCDIVRAGL
                     LAHNTLKEGSKLEHYYTIPKFTRERITDLIGNQFDAMLDWIEAYQKDSPLPLDIFLSR
                     FFGELLSQPGFGLHANADAASTLNALIQSARDFRLMFSSLAENQRQSISEMYIQTLQS
                     GLLPSSHSFLSEEEHAVAISPAFTFLMKDRTVNYQFWLDIGNIGWWQRLDQPLTHPYV
                     LNRNWSREKRWTDAEEFQSNQAVLARLVQGLLERCKKHVYLYVTGMNQAGINQNSPLL
                     SGMQLFLKRSYRMEIHE"
     gene            6794..9013
                     /locus_tag="PLL88_04560"
     CDS             6794..9013
                     /locus_tag="PLL88_04560"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOJ00868.1"
                     /translation="MSEFIPRPAQADILRYTHGKMGIAAVPGSGKTHTLSYLAATLIA
                     SDRMKDGQEVLVVTLVNSAVQNFSKRISSFIKQFKLIEQIGYRVRTLHGLAHDIVRER
                     PDLAGLENNFQILDERASNDTLDQIVTTWQRNNPAFLESYTHPDSLENMRYLANNWKL
                     VLRSMASNFIQQAKDMELTPTQITSGMAKNQIEHDLLRFGIEVYRDYQNALSYQAAID
                     FQDLIRLAYHILQTDHDYLRRLQERWPYILEDEAQDSSALQEKTLRLLCGESGNWVRV
                     GDPNQAIYETFTTADPHYLLDFLKEKNVQPRDLPNSGRSTASIMRLANHLIEWNQTQP
                     APELTRALVPPLIQPTGVDDPQPNPPDAPEKIFLFEKQLTPEREEALVVQSVARWLQE
                     NPHQTVAILVTRNDKGASLVQKLKDRRIPTLELLQTSFATRKTAKILASLISFFTDPL
                     SSKKLDAAFEAWCAEQFEEPLLKTAFHAFRNDLKKLTKLEDVLFGKNELLPEASEPEQ
                     HAVREAFRHFVEFFKENQHAALLPVNQFLLTLSMRLFTDSRALALSYKLAEMMEWASR
                     SNPAFSLADCAADLDKVYHNRLRINGFSEEELEFDPDQHKGEVVVATIHKAKGLEWDR
                     VYIVSVNNYDFPSNQPYDQFISERYFVRDHLNLEAELLAQLKALFAKKTVTEGDATRL
                     ARISYSAERLRLLYVGITRARQELVITWNEGKYNNGIMAIPFVELHTFWEQYHAAHT"
     gene            8997..9830
                     /locus_tag="PLL88_04565"
     CDS             8997..9830
                     /locus_tag="PLL88_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOJ00869.1"
                     /translation="MPHIPDFRFSSQSLQDYVECQRRFALRYLQRLDWPAEESSPYLA
                     FEHHRQKGNLFHTCVERYFHQVDPALIEKHIADDDLRDWWQRFLRFMDGKKITFSAPE
                     ILFQTSLRDHVLIAKFDLLVKNDENTFTIYDWKTIAGEKRPTRAVLAEKMQTLVYPYV
                     LCASATPEGDRQKISPAQVELIYWFPQFPTDPEKFAYSSVQMQENEKVLLALMDEIAQ
                     KSKDDFPKTEDEKRCRFCVYRSYCGRGSQAGSFSGRDEEFDLELEELDLDIETAEEIA
                     Y"
     gene            9827..12160
                     /gene="recJ"
                     /locus_tag="PLL88_04570"
     CDS             9827..12160
                     /gene="recJ"
                     /locus_tag="PLL88_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOJ00870.1"
                     /translation="MNKRWVSPEPIVIPDSLQEYFAVSPYLAEALAKKGITDPSSALP
                     FLDYTKYQPASPHELPGVEKATQRVLQAVKKKESIGIWGDFDVDGQTSTALLVSFFRQ
                     LGLQVQYHIPIRAEESHGILKEPLQNFLARGIDLLITCDTGISAFPALNFATHSNVDV
                     ILTDHHTPSHLLPEAFAIINPHFVSEGHAFSDLSGVGTAFILTRSVAAELNMQSQWEK
                     QAIDLVAMGTIADVAPLVRENRYMVQYGLDRMRAHPRLAVQEVLSVAGTPAERVDEQN
                     VSFTIAPRLNALGRLGDANPMVEFLLTEDIALARTYATHLEGLNNQRKLLVNQVYQAA
                     LAQIETDPNQERRSILILSNPAWPAGILGIVANHLVEAYGKPAILLTANASGVFQGSA
                     RSLAGINITELLQDNEDLLLSYGGHAMAAGLALKKENLDRFVQNMNASVAAIPEAQRM
                     EATLPIDAYLPLENVNEPFMRVLDSLAPFGAGNPAPLFLAERLQITGSRAIDRDKVHR
                     SIQVQTAQGQPYEVKWWHAFDAPLPTGEIDLAYSIRRSVFRGEESIEIGLVDMRASTV
                     EEPVEVAARPAVEIADFRFSRNPQEDLREYLQSKQPVCWAENLETNLVESYNRLDVPS
                     HAALALLHMPPSAEVLQNILQKVQPLSIALFDLPQKPLRGKRFLQTLLGMVKFAMREK
                     QGKLSLQHYAALTGFTTEIVKIALSLLEADGQITIQREHEDNLSVARGNQTADIDALR
                     TCQEKLVHAFKVMQAFQRFLLRLPPEQILSDYYERGN"
     gene            12203..13234
                     /locus_tag="PLL88_04575"
     CDS             12203..13234
                     /locus_tag="PLL88_04575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOJ00871.1"
                     /translation="MSTISRRDFLKNMGAGVVSLALTGHSEKSNARSSTQLGRVAKDT
                     SISVYSQASENSAIRFQRYRDEIFHIYDEVVAPDGPGYNPLWYRIWGGYVHSGFVQKV
                     QYRLNTLITEFPSQGQLMELTVPYSDSYRHRINEKWEKFYRLYYSSTYWVFSAKEGPD
                     GMLWYEIRDGLVDLSYYVPVEHLRPVTAEELTPIHADIAPSLKRIEISIDFQKLMAYE
                     GSQLVKEMRVSTGVPYLSSAPNTIPTDTPKGNFKIHAKRPSVHMGDGTLRSDAEAYEL
                     PGVPWVSYFESSTGVAIHGTYWHQNFGMVMSHGCINMESEDAKWVYRWTNVPDDGNSE
                     GYRTPVLVY"
     gene            complement(13412..13549)
                     /locus_tag="PLL88_04580"
     CDS             complement(13412..13549)
                     /locus_tag="PLL88_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00872.1"
                     /translation="MACHPYWLVSLAHGEIPRIARDDVMDRIVGTLLFGWLEWDLDWI
                     A"
     gene            complement(13669..14853)
                     /gene="hemW"
                     /locus_tag="PLL88_04585"
     CDS             complement(13669..14853)
                     /gene="hemW"
                     /locus_tag="PLL88_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOJ00873.1"
                     /translation="MKEAGIYVHIPFCKSRCRYCDFNTSAGMEHFIPEYFSSLEKEIK
                     IYGERYASLYAIRSIYAGGGTPSYLAAGTIPRVIQEIKRSFHVTTDVEISVEANPADI
                     TMEKCREWTHAGVRRMSIGMQSAQEGELQMLGRRHCFADVVRAVEQVRSAGMTNFNLD
                     LMFGLPHQSMKDWQDSVKKAMLLQPAHLSLYALTVEEGTPLAIDMDAGLLPAPDDDLA
                     ADMYEWATDFLATQGFEQYEISNWAKQDGVNYHCQHNLLYWRNQDYFGFGASAHSHFG
                     ARRWANLNGIQPYVKGIALYSAGEEAGFPWLETNTALSVADDMGETAMMGLRLTQEGL
                     RDDLFQERFGISLFRVYEKQIAELVDIGLLEVVSGDMSAIRLTKRGRMVGNQVFLRFL
                     MV"
     gene            14923..16062
                     /locus_tag="PLL88_04590"
     CDS             14923..16062
                     /locus_tag="PLL88_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ00874.1"
                     /translation="MNSKNKPSTEKVCPTCGTHLSVNATRCLVCGRTFTDAPASTPAN
                     KIKPRGLPQFTVSLPIALGAVLILIALGAGIIYFVLRGSGQITAAQPTATLTPTPTLE
                     QSPTPTLTATPAPTATLEPPIEYSVQSGDTCSSIALQFNVTVKSIADLNNLASDCGVL
                     TLNQTLLIPRPTPTPSPQPTGTLSVAQKTESACTKITYTVGANDTLSAIAANYNVSVE
                     TLKTYNGKTSDNVYLNEVITIPLCERLPTSGPTPTATLPAPFAATNLLLPADGAVFNA
                     THDTVTLQWAAIGGMLEGDSYAVTIEDLTAGEGKKLTDYTKDTKYIVPVTFRPAESAP
                     HVIRWYIQPVRQSGTTTGGDPIYVTAGYPSDPRVFIWSGLGTSSN"
     gene            complement(16097..17032)
                     /gene="trxB"
                     /locus_tag="PLL88_04595"
     CDS             complement(16097..17032)
                     /gene="trxB"
                     /locus_tag="PLL88_04595"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOJ00875.1"
                     /translation="MENNEKDIESVIILGSGPAGLAAALYAARAELKPLLFTGTEVGG
                     QAATTSVIENYPGFPDGVEGGQLAELFTKNAEKFGARIEYDMVTAADLSKSPFKVSTY
                     GKDHYAQVLIVTTGASANKLNVPGEKELTGRGVSYCATCDGWFFKDKKVAVVGGGDSA
                     LEEGLFLTRYASSVTIIHRRDELRAGALLQKRARENPKISFIWDTVVEEIKGEGAVSG
                     LQLKDVKTGETRELAIGGVFVFIGHSPNTELFRDWLKLDEKGYIRIDQNMQTSVAGVY
                     AAGEVADSRYRQVVISAGMGAAAAIEATRFLESQE"
     gene            complement(17050..18393)
                     /locus_tag="PLL88_04600"
     CDS             complement(17050..18393)
                     /locus_tag="PLL88_04600"
                     /inference="COORDINATES: protein motif:HMM:NF004718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ00876.1"
                     /translation="MAQGKNQAMIDRCMAHNFWTWSRQKEIQPIPVERAQGVFFWDFD
                     GKRYLDFNSMVMCSNIGHGNQQVIDAICKQAQELTFAGPHMATEARARLLEKLCEVVP
                     QGISRFLFTMGGADANENAIKIARAVTGKHKILARYRSYHGASHGAVALSGDLRRVPW
                     EPNVMGGVVHFPATYRYRDDLVPDAMQLADEEYAARHLEYLEKLIQYEGADSIAAIFL
                     EAVTGTNGILIPPDGYLQGIRALCDHYGILMIVDEVMSGFGRTGAWFAVDHWQVKPDL
                     ITMAKGLTSAYAPLGAVGMREEVAAYFDRRKFDSGLTYNGHPISFAAATANIDVLQEQ
                     KLIRHTQAMQPILLKAFNRLKEKHPAVGDVRGIGLFGAIELIKDKKSETPLGEYGKTP
                     TIMNELQKFTLSKGLFHYVHDNLILVIPPLIVNEEELAMGITILDEALKTTDTVL"
     gene            18500..19285
                     /gene="fabL"
                     /locus_tag="PLL88_04605"
     CDS             18500..19285
                     /gene="fabL"
                     /locus_tag="PLL88_04605"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HOJ00877.1"
                     /translation="MCENTAMIFEGKIALITGSSRGIGQAIALHFARLGAHVVINYVH
                     NQEPAEETARQIRAMGRKALVVRANVGKIEHLERLFQTVEQEFGALDIFISNAASGFN
                     RTALQQRPEGWEHTMNVNARAFLFGTQLAVPLMEKRAAGKIVAITSQGSVRVMPDYVS
                     VGASKAALESLTRYLAVELAPKNIAVNAVSPGVVETDALQHFASLSDPTVLQRAIQHT
                     PVGRITTPQDVACMVAFLCSEQAFMIRGQVIVVDGGFSLPVPH"
     gene            complement(19282..19914)
                     /locus_tag="PLL88_04610"
     CDS             complement(19282..19914)
                     /locus_tag="PLL88_04610"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOJ00878.1"
                     /translation="MIIDISIELSPEMVRWEGDPPMIVRQVLQLAKGDPYNLSEMKLS
                     LHQGTHLDAPRHFLADGSLVEDFPLDLLIGEAQVVQIPCEVNSIGRDVLEKAGIRAGI
                     RRVLFKTRNSQYWKDDPHTFHAEFVSLSLDGAEYLIEQGIELVGIDYLSISPADDFRP
                     VHARLMEKHIAIVETLDLSEVEPGAYTLICLPMKIQGVEGAPVRAVLMHD"
     gene            20017..21162
                     /locus_tag="PLL88_04615"
     CDS             20017..21162
                     /locus_tag="PLL88_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HOJ00879.1"
                     /translation="MREVAAIGIGQTKIEEHWDKSLKEIAGEAIFAAIQDCGIDHVDG
                     VFVGNMMSASANSQQNLGAYITDWIGAKHAGALRLESACGSGAAAFRAAFLAVTSGEA
                     EVALAVGVEKMTDSPTAEITSHLATAASADWEVDQGLSFVALNALLMRRYMHETGWQH
                     KDFAPFSINAHTNAMHNPFARFHMELTEQKYLEAGMICDPINLMDASPIGDGAAAIVL
                     VPADKVSEKVIRVVASAAATDTIAVHDRKDPTWLSAAEVSSKKAYAQGGLAPQDIDFF
                     EYHDAFTIMAALSLEACGFIERGKGPLMALEGHIGKNGRLPVATMGGLKARGHPVGAT
                     GAYQLVEVIQQLRGTAGENQLEKARVGMAQNIGGSGSNIITHILRND"
     gene            21155..22024
                     /locus_tag="PLL88_04620"
     CDS             21155..22024
                     /locus_tag="PLL88_04620"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOJ00880.1"
                     /translation="MTDLDTLRKRVISYREKTYRTARSLRLHSPQDALDFFNERGFIF
                     FWPIKDYDLPNLWYATVGARPVPNNHDDPGHVTWGWKDGMLDKRAWYYAKLVRKKATI
                     LSLEMAPYFYALSPNYGDYENDYLLQYKSGQMSVEMKNVYEALLENGPLHTLDLHKQA
                     HLTSSDKKYLFDKTLTDLQAEMKIIPVGIAVAGRWGYAMIYDIAARYYPEIVEKARFI
                     TEASAQQAILLSYLRSVGMADLVTIKRMFTWKEPFTSQACAQLVEQNHITAIFDADRS
                     NPLYFINDIFHLQ"
     gene            22032..23834
                     /locus_tag="PLL88_04625"
     CDS             22032..23834
                     /locus_tag="PLL88_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ00881.1"
                     /translation="MKSIPRSASPASDHASRLPLAHASEQPSPEAPWKKNVVLLAKNV
                     NVWLAQLSGKYRQKIVHLDHIPAVELEKLAAWGITALWLVGVWERSPASRKIKELYGR
                     EDIAASAYSIKEYRIARSLGGEPAADALHAACDRAGIRLCVDIVPNHTGIDSDWLLEH
                     PEWYISVSENPAASFHFNSPDLSPTPNISLQLEEGYYDQSAAAEVFLYTDHRTGEKRF
                     IYHGNDGSSMPWNDTAQLDYLNAEVREHVIETILCILKRFPLIRFDAAMTLTRQHFQR
                     LWYPLPGSQERCVPTRERHALPAEAFLKAKPREFWREVVERVERENPDALLMAEAFWY
                     MENFFVEELGMHRVYNIAFMQSLRDEDNKTYQSILKNALASNPLLLGKFVNFLSNPDE
                     RTALDQFGKGDKYFAACTLLVTMPGIPMFNHGQIEGFSERYGMDFLLPLLHEKEDADL
                     LRLHERWIFPLLRMRPCFSDAASFCLFDVVGQAQRSLPHIYAYLNHYHDRYFLVLVNN
                     SSRNQAIRFRRTVPLRGTSGKPAVSTLLEWLPRSADPQANLRCREVRTGEEWSFAYQD
                     LEETGISLKLKPYQHLVWELFWSEPPLHSAPSSR"
     gene            complement(23758..>24591)
                     /locus_tag="PLL88_04630"
     CDS             complement(23758..>24591)
                     /locus_tag="PLL88_04630"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOJ00882.1"
                     /translation="YSPQLPHSIPVEVAVSSMQDERGIRAALKDVNCIFHLASSEKEG
                     QHIDLEKSDIPGLENLLTAAEQVGVERIFYLSHIGAERTSGFPAFRAKAVQEKMLQNS
                     KATATIFRTSVVFGEQDGFLSYFARGLAASKFIYAIPGSDDILMQPLWINDLINCLYL
                     AYQNEDYAGKIYELGGGEYFTFRAILQMIMERIGKKRYLVPIAPAALHARNLWFGESP
                     SGFTLSTFWIDYLASDRTCSLDSLPHQFNILPGRFSEKMERYVAVVRSRKAPRQGAGR
                     V"
BASE COUNT         5891 a         7035 c         5846 g         5819 t
ORIGIN      
        1 gagcgccgct gtcgctgcca ccaacgcagc taccccgttc accaagaaca catagctttt
       61 caatggcaac gcgtcgcgca ccttgcgccc caccatcgta tacgctgctg ccatccatgc
      121 tccaactaga gccaggaaat ttcccagcag gctccgccaa tcgaaagtcc cgccctgcgc
      181 tcccccctgc ggtccaagga aaagaacaac gatccccagc atggcaatca tcaaccccag
      241 catcacattc ctggtgatcg tctctttgag aaccagagga gaaaacaatg ccacccagat
      301 gggagtggtc gtaaccaaga tcacggaact agccacgctg gtcatttcca acgaaatgat
      361 ccataccaaa aaatggatcc ccaaaaagaa accagccaga atgattggtg atcgttgggc
      421 gcgtggaagc gcaaaaaatt ccctcaacgc tctcttccct ccaaaaggcg ccaaaatcaa
      481 cgtcgcgctg atcaggcgcc ctgccgcaat cactgtggat ggcagggttc cctgggcttt
      541 tcggatcaat aaagaagccg tggatactgc caaaattccg agaaaaaggt tgatgcctgc
      601 tttattaaga gatttcatat attcttgctg tctggtttag actatagtta tccttaaaac
      661 aaacaataga taattcattt gtgaggagga aaagatgagc tttattttac ccgaacttcc
      721 ctatgcctac gatgccctgg aaccccatat ggatgcccag accatgcaga ttcatcatga
      781 caaacaccac gctgcctaca ccaccaactt aaacgcagcc ctggagaaat atcccgagtt
      841 ttttgaaaaa tcgatcgaaa ccatcctgtc tgacttgaac gccattcccg aagaaatccg
      901 caccgcagtc cgcaataacg gcggtggttt ctaccatcac aacctgtggt ggacagaact
      961 cgcccccaat gcaggcggtg agccgattgg aaccctggca gaagtcatca aaaaggactt
     1021 tggcaacttt gctacattca aagaacagat gggcgcggca gccatgggac gttttggcag
     1081 cggctgggct tggttgagca tgcgcagtga taagaccctc atcatccatt ccacacccaa
     1141 ccaggattca ccgctctcgc agggtctgct ccctctgttg ggaatcgacg tgtgggaaca
     1201 tgcctattac cttaaatatc aaaacaggcg caaagatttt gtggatgcat tttggaatgt
     1261 cgtgaattgg gatgaggtgg aacgtcgctt cgccgaagaa aattctgccg catcatgaac
     1321 gctgggtagc atgaaggagt agccgttcta ctccttcact tttttccatg ttcggcatac
     1381 catgctgcgc cgatgatgcc agccaggttg agatgttcag ccggtaagag cttcgcattc
     1441 acgcgcaaat gaggaaagaa cttttcatgg tatttgctcg ccccacctcc gatgatgaac
     1501 acatctgggc tgaaaaggag ttcaagatat tcaaggtatt cctgcagcaa aattgaccat
     1561 tctttccagt gcaggctttt cttttggcgc accgcatccg aagcgcgttt ctcggcgatt
     1621 tttcccctca tctcgagatg ccccagctct gtgtgtggca acagttctcc ccgcacaaag
     1681 atggctgaac caatacccgt ccccagagta agcacgatca cgatttcttt ctgaaatggt
     1741 ctgccggctc cgaagtacat ttccgctatt ccggcggcat ccgcgtcatt gacggtagct
     1801 gtgtgcagtc cggtcttttc aaagacctcc tgattgatgt tcatgcctat ccactttttg
     1861 ctgatgttcg ccgcgctcaa cgccacccca ttcttgacga tcgccggaaa accgatgccg
     1921 atggttccgc tccattgcag gtcattgaca aaggtcgcga tgagatcgag cacctcttct
     1981 ggtttgatct ttccatcgat tggataccgc ttcctttcgg tgagtaattg ccccttacgg
     2041 gtgtctacca gcgcaccttt gattccggtt ccacctacgt cgatccccag cactttagcc
     2101 atattttctc ctactggcag gcattggatt gatggtcctc gtaactttca gcaaaaacat
     2161 gcgccccact tccatcgcaa gccgagcgaa aatagaaata ttccgtgtct gccggaaaag
     2221 ccaccgcaat caagctatct acgctcaccg cacaaatggg cgtggggggc aatccgttga
     2281 ccctgtatgt attatacgga gaaaataccg ccaggtcagc gctggtaagt ggtgttttcc
     2341 accagcttcc ggtttgttca tcccacccca gtgcgtactg cacggtaggg tcactttgca
     2401 gcaacatgtt cacctgcagc cggttcaaaa agaccgatgc aatggacggt ttttcctcat
     2461 cctttactgc ttcgcgctcc accaccgacg ccaatgtcat cgcctggtaa ggcgaaagcc
     2521 cctgccggat gaacgcattc accacttcag gggtgaactg tgcagccgat tgctcaatca
     2581 gatattgcag aaattctgcc gcactcatct cgcgcaagat ggtataatcg cccgccaaaa
     2641 tcagcccttc cagatttgac gcaccggcaa aaatctcagg gatgtcgatc ccgctccttg
     2701 tttcgatcag agaaagcagc tcctcagagg tgaaattcaa acctgaggta ggaataagct
     2761 gcacgatctc ttccaatcgc attccgggca agatcacaaa attcacatcc tcaggggttg
     2821 gatcgatcat agctaatgcc agctcagtcg gggcttgcgt tctggaaaag taataggtgc
     2881 cgctctgtaa aatgcgatcg taccccttat aaacaaggta acgcaaaaaa ctgctttcgt
     2941 ctgctatgaa gccagcctgc gccaggtttg tgctgacctg gctggcagtt tctcccgtta
     3001 aaatagagaa ttcctgtttt tctccagaat ccgcttccat ggctttgttc aggtcctcgc
     3061 catggagata gagccatacc gccaggcgcc tggattgaaa gaaattcaaa ttcgcatccg
     3121 cttccccaaa ccgtgctctg gtttgatcag ggaaataaaa cagaaatagg tatagggttg
     3181 aaaagagaat caatgcaaga agaacgatcc acaaatacac actttttttt cgcattgtta
     3241 ttctcctctt tcaaaatgag aatcgatata ggattgcaac aagatgcagg ctgccacatc
     3301 gtccagatgc ccgcgccgcc gcttgcggct tatgcccatc tctcggcgtg tctgctgcgc
     3361 gatccgcgtg gtgtccgcct catcccaata ctctatagga acgtcagcga atccttgaat
     3421 ggcttcaccc aatcgctgtg ctttccgtcc ggaaaaagtc gcttcccctt tttcatcacg
     3481 gctgatgccg atgacgatct ttttgatgtc cattgcgaag caaattttca agatctctct
     3541 ggcactttcc cggtaggaag tatggcgaat gacgcgcagc ggtcttgaga tcgtgccgct
     3601 tgggtccgaa atcgaaatcc cgatgcgctt ctcaccggga tcaacaccca gcaagcgctc
     3661 attacttcta ttcttttcca tccctggcat tatacattaa ctgaagagca gaaaattctt
     3721 ctcaaaaatc aaacgtatta aaatatgctg aaatctcggc aatttttcac ggatttcctt
     3781 ataataggac tatgaacaaa aaatatcctt tcaatgttcg cgttgtactg ttgatcgggt
     3841 ccctgctgtt tttattgcaa tcctgcaatt tccctttggc tcaaggtaat tcgtttaaca
     3901 agcaggtcgc tacccaggct gcgttcgctt ttacccaaac agccgaacaa agtcaaatgc
     3961 ttaccgctaa cgcccccacc acgcctaccc cggcgatttc cgccatcact cccaccagtg
     4021 atctggatga ccccaaagcc cagttgggca gcgctgcctg gcaagattcc ctgaccagtg
     4081 ggaacagctt tggtgtagga aacacggaag agcagatcag tggcacgaat gcaaccgtat
     4141 gggtgaaaga gggcagtttc aatatgtacc gcccgactgc gtcaggaggc tacatctggt
     4201 actgcgccta ccccaatatt gcagactttt atctcgaagc cacctttacc gtgcaaaact
     4261 ccaatggcac cgacgaatac gggctgttta ttcgcaaacc ggacttcacc gataactccg
     4321 gttactactt cattgttact ggcgagggta aattcaacct catgcgctgg accgccagcg
     4381 gttccacttt gctcggggaa tggaaaaccg acgagaatat catcaaagga cctgatgcca
     4441 caaacaccct ggggatttgg gctgagggaa atacgatcag gttgtacatc aacgaccact
     4501 tcgcaggtga attcaatgac acccttggct gggcatccgg gtattttggt ctattcacca
     4561 acgccaggca aacagccggc atgctggtca agatggatga gatcacctac tggaatttgc
     4621 cctagaaaac aagaagaaaa ccgcatctcc tgttgcaact cactggaatg atgccaactg
     4681 tttcccgata ggatgaaagg gatggaccat ctgcatatca acgctcaaca acaagctacc
     4741 ctggatgcgg agctgatttc tcacgtcttt ttggaaggtc cctataaaag cggaaaaacc
     4801 accatcgggt tggcgcgctt gcgtgaggcg gctgcttctg ccgatcctgc ccaccaagtg
     4861 ttggtgttga ctccccagcg ctcgcttgcc agaccttatc gcgatgccat cttgaagccc
     4921 gattttccgg ctggcgtttt gcctgctctg accacctacg ccgggcttgc gcgccagttt
     4981 attcgtttgc actggccgca gatttctctc cacagcaaat tccgcacacc cgctcaatcg
     5041 cccaccttct taagcatgga aaccgcccaa tatttcttgc ggcagctatg ccaacctttg
     5101 ctcgccagag gatacttcct cgaaatccgt atcgatcagg ctcgcctttt cagccagatt
     5161 ctggatacca tgaacaaagc cgccctggtg gggtatcctc tgcaagaaac cgcccaacgg
     5221 ctgaaggatg cctggaatgg ggaagccatt cgggaaaagc attatgagca aacccaggaa
     5281 tgcgcgcaag cctttcgcga ttattgcctg caaaataatc tgctcgattt ctccctccag
     5341 gtggaagtat ttcgtgatca catggaacag gatccccaat tgaaaaaaga attctttcga
     5401 aactatagct tcctcatcgc cgataacctg gaagaagacg ttccggtcat gcacgacctg
     5461 ctcctttctc tgacaaatga tattccttct atgttgctta tcaaagacag tcagggcgga
     5521 tatcgttcat tcctgggggc tgatgccctc agcgcggagc gcttgaaaca catctgccag
     5581 cacacgttca ctttcaccga tcagtttgat cttgacaccg ccgttcaagc gttcaatgtg
     5641 tccctctccc attgcattct gcgcaaagat acgtcggacc tgccatctga atcacgcaac
     5701 gcgtttaccc tatgcaatgc gcagttctac ccccagatga tcaccgacgt ctgccacacc
     5761 attcaccagc ttatccaggc tggcaaggca gatcctgcag acatcgcggt tctatctccc
     5821 tacctgcccg attcgctgaa gtttgcactt tcccaaaaat tggcgcaagc gggaattccc
     5881 ttcctttcct ctcgcccctc tcgctcgctg gctgaagaac ccgtcacacg tgctgtcctc
     5941 gcctttgcga agatcgccca tccgcagtgg aacatgggca tttccgttga ggaactccgc
     6001 cataccctga tgaccgtttt tcccacctgc gatatcgttc gggctggtct gctggcgcac
     6061 aacacgctca aagaaggctc caaactggaa cattactaca ccatcccaaa attcacccgc
     6121 gaacggatca ctgatctgat cggaaaccaa ttcgatgcca tgcttgattg gatcgaagcg
     6181 tatcaaaagg attcccctct tcctctggat atttttctct cccgtttctt tggagagctc
     6241 ctttcccagc ccggttttgg attgcacgca aatgcggatg ctgcctcgac attgaacgct
     6301 ctcatccaat ctgcccgcga ttttcgcctg atgttctcat cattggctga aaaccagcgg
     6361 cagagcatca gcgaaatgta catacaaacg ctacaaagcg ggctgctgcc atccagccat
     6421 tctttcctgt cagaagaaga gcatgccgtc gcaatttcgc cggcgtttac ctttctgatg
     6481 aaagatcgca ccgttaacta tcagttctgg cttgatattg gaaacatcgg ttggtggcaa
     6541 cggctcgacc aaccgctcac ccatccctac gtcttgaatc gcaactggtc tcgagaaaaa
     6601 cgttggaccg acgcggaaga atttcaatcc aatcaagcag tgttggctcg cctggtgcag
     6661 ggtttgcttg aacgttgcaa aaagcatgtg tacctgtacg ttaccggtat gaaccaggca
     6721 ggcatcaacc agaacagccc gctgttgagt gggatgcagt tgttcttgaa aagaagttac
     6781 aggatggaaa tccatgagtg aattcattcc tcgtccggct caagccgaca tcctgcgtta
     6841 cacccatggc aaaatgggta tcgctgcagt tcctggcagc gggaaaaccc acacgctctc
     6901 ctatttggct gccaccctca tcgcttccga caggatgaaa gatggacagg aagtgctggt
     6961 tgtgacattg gtgaattctg ccgtgcagaa tttttcgaag cgcatttcca gcttcatcaa
     7021 acaattcaag cttatcgaac agatcggcta ccgggtgcgc accctgcatg ggcttgcgca
     7081 cgatatcgtg cgcgaacgcc ccgatcttgc cggattagaa aacaattttc agatcctgga
     7141 tgagcgcgcc agcaacgata ccttggatca gatcgtgacc acctggcagc gcaacaatcc
     7201 tgctttcctc gaaagctaca cccaccccga ttcgctggag aatatgcgct accttgcgaa
     7261 caattggaaa ctggtgctgc ggtcaatggc atccaacttc attcagcaag ccaaggatat
     7321 ggagctgact ccgacgcaga tcacctctgg catggcaaaa aaccaaatag aacacgacct
     7381 cctccgtttt ggaattgagg tttaccgtga ttaccagaat gcgctgagct accaggcagc
     7441 catcgacttt caggacctca tccggctggc ataccacatt ctgcaaaccg atcacgatta
     7501 cctgcggcgc ttgcaggaac gctggccata tattctggaa gatgaagcgc aagattcctc
     7561 cgccctacaa gaaaaaacct tgcgtttgct gtgcggtgaa tcggggaatt gggtgcgcgt
     7621 cggcgacccc aaccaggcga tttatgaaac cttcaccaca gccgatccgc attacctgtt
     7681 ggattttctc aaagagaaaa atgtgcagcc tcgcgacctg ccaaattctg gcaggtctac
     7741 cgccagcatt atgcggcttg ccaatcacct catcgaatgg aatcaaaccc agcccgctcc
     7801 agaattgacc cgggcgttgg tgccgcctct catccagccc accggagttg acgatccgca
     7861 acccaaccca ccggatgcgc cggaaaagat attcctgttc gagaaacagc tcacgccaga
     7921 aagagaagaa gcgctggtgg tgcaatcggt ggcgcgctgg ttgcaagaaa acccgcacca
     7981 aaccgtcgcc attctggtaa cgcgcaatga caagggcgcc agtctggtgc agaaattgaa
     8041 agaccgccgc atccccacgc tcgaacttct gcaaaccagt ttcgctacac gcaagaccgc
     8101 gaaaatatta gcgtccttaa tatccttctt cactgatccg ctcagttcca aaaaacttga
     8161 cgccgccttc gaagcatggt gtgccgaaca gtttgaggaa ccgctgttaa aaaccgcctt
     8221 tcacgccttc cgcaatgatc tcaagaagct aaccaagctg gaagatgtgc tgtttggaaa
     8281 gaacgagctt ttgccagagg catcagaacc agagcagcat gccgtgcgtg aagctttccg
     8341 ccatttcgtt gaatttttca aagaaaacca acatgccgcg ctgcttccgg tcaaccagtt
     8401 cttgctgacg ctctccatgc gtctcttcac ggattcgcgt gcattggcgt tgagttataa
     8461 actggcggag atgatggaat gggcttccag atccaacccc gccttctcac tggcagattg
     8521 cgccgcggat ttagataagg tctaccacaa ccgcttgcgc attaacggct tttcagaaga
     8581 ggagttggaa ttcgatcccg accagcacaa aggggaagtg gtggttgcca ccatccataa
     8641 agccaaaggc ttggaatggg atcgcgtcta tatcgtctct gtcaataatt atgattttcc
     8701 cagcaaccag ccctatgacc agttcatttc agaacggtac tttgttcgcg atcacctcaa
     8761 tttggaggca gaactgctgg cacagctcaa ggcattgttc gccaagaaaa cggtcacaga
     8821 gggagatgcg acgcgccttg cgcgcatcag ctacagtgca gaacgtttgc gtttgctcta
     8881 cgtgggcatc acccgcgccc gccaagagct cgtaatcacc tggaatgaag ggaaatataa
     8941 caatggcatt atggctatcc ccttcgttga attgcacact ttttgggagc agtatcatgc
     9001 cgcacatacc tgattttcgc ttcagttcac aatctctgca agattatgtg gaatgccagc
     9061 gccgttttgc cttgcgctac cttcagcgct tggattggcc agccgaagag agcagccctt
     9121 acctggcatt tgagcatcat cgccagaaag gcaacctctt ccacacctgc gtcgaacggt
     9181 attttcatca ggtggatcct gctttgatcg aaaagcacat cgcagatgat gatctgcgcg
     9241 actggtggca gcgctttttg cgcttcatgg acggcaagaa aattaccttt tccgctcccg
     9301 aaatcctttt tcaaaccagc ctgcgggacc acgtcttgat cgcaaaattc gatttgctcg
     9361 tgaaaaacga cgaaaacact ttcaccatct acgattggaa aaccatagcc ggtgaaaaac
     9421 gacccacacg tgccgtcctg gctgaaaaga tgcaaacgct ggtctatcca tacgttttgt
     9481 gcgcatcagc cactccagag ggagaccggc agaagatttc ccctgcgcag gttgaactga
     9541 tttattggtt cccgcagttc cccaccgacc ctgaaaagtt cgcgtactca tctgtgcaaa
     9601 tgcaggaaaa cgagaaagtt ctccttgcgc tcatggatga gatcgcgcaa aaatcgaaag
     9661 atgactttcc gaagacagag gacgagaagc gctgtcgttt ctgcgtttat cgctcgtact
     9721 gtgggcgcgg cagccaggca ggaagtttct ccggcagaga tgaggaattt gacctggagc
     9781 tggaagaact ggatctggat attgaaacag cggaggagat cgcctattga acaaacgctg
     9841 ggtttcccct gaaccgatcg tcatccctga cagtttgcag gaatattttg ccgtttcacc
     9901 ctacctggct gaagccctgg cgaaaaaggg catcaccgat ccatccagcg cgctcccttt
     9961 cctcgattac acaaaatatc agcctgccag cccgcatgaa cttcctggtg tagaaaaagc
    10021 aacgcagcgt gttctccagg cggtcaagaa gaaggaatcc atcggcatct ggggtgactt
    10081 tgacgtggac gggcaaacct ctaccgcctt attggtgagt ttttttcgcc agttgggtct
    10141 tcaggtacag tatcatattc ccattcgcgc tgaagaatca catggcattc tcaaagaacc
    10201 tctgcagaac tttctggcgc gcggtattga cttgctgatc acttgcgata ccggcatttc
    10261 agctttcccg gcattgaact ttgccaccca tagcaacgta gatgtaatct taaccgacca
    10321 tcacacccct tctcacctcc ttccggaggc ttttgccatc atcaaccctc attttgtatc
    10381 ggaaggacat gctttctccg acctcagtgg ggtgggcact gccttcattc tcacccgttc
    10441 ggtagctgcc gagttgaaca tgcaaagcca gtgggagaag caagccatcg acctggtagc
    10501 catgggcacc atcgcagatg tggcacccct ggtgcgggaa aaccgttata tggttcaata
    10561 tgggctcgat aggatgcgcg cgcacccccg cctggcggtt caggaggtac tctctgtcgc
    10621 tgggacaccc gcagaaagag tggacgaaca gaacgtcagc ttcaccatcg cacctcgttt
    10681 gaatgcttta ggacgcttgg gagatgccaa tcctatggtt gaatttttgc tgacggagga
    10741 tatcgcgctt gctcgcacat acgccacaca cctggaagga ttgaacaatc aacggaaact
    10801 gctggtgaac caggtttatc aagcggcact cgcacaaatt gaaacagacc ccaaccagga
    10861 acgacgctcc atcctcattc tctccaatcc cgcctggccc gccggcattt tgggaattgt
    10921 cgccaatcac ctcgtagaag cctatggaaa acccgccatc ttgcttactg cgaatgcaag
    10981 cggcgtcttc caggggtcag cgcgttctct tgcggggatt aatatcacag agctgctgca
    11041 ggacaatgaa gacctgcttc tctcgtacgg cgggcatgcc atggctgccg gtctggcgtt
    11101 gaaaaaagag aacctcgatc gcttcgttca aaatatgaat gcgagtgtgg cagccattcc
    11161 agaagcgcaa aggatggaag ccaccctgcc tattgacgct tatctccctc tcgaaaatgt
    11221 taatgagcct ttcatgcgcg tcctggattc gctcgctccc tttggagccg gcaatcccgc
    11281 cccgctattc ctggcagaac ggttgcagat aaccggttcc agggctatag accgcgataa
    11341 ggttcatcgc tctattcaag tgcaaactgc gcagggacag ccctatgaag tgaagtggtg
    11401 gcacgctttc gatgcccctc tccccactgg agagattgat ctggcataca gtatccgccg
    11461 tagcgtgttc cgcggcgagg agagcatcga gatcggtttg gtggacatgc gcgcttcgac
    11521 cgtggaagag ccggtggaag tggctgcgcg accggcagtg gaaatcgccg atttccgctt
    11581 ttcgcgaaac ccgcaagaag atcttcgaga atacctgcaa tcgaaacaac cggtctgttg
    11641 ggcggaaaat ttggaaacca accttgtgga aagctataac cggctcgacg tcccttcgca
    11701 tgcagccctc gccttgctgc acatgccgcc ttcggctgag gtgctccaaa acatcttgca
    11761 gaaagtgcaa cctctttcca ttgcactgtt tgacctgccg caaaaaccgc tgcgtggcaa
    11821 gcgatttctg cagaccctgc ttggcatggt gaagttcgcc atgcgtgaaa aacagggcaa
    11881 gttgtcgttg cagcattatg ccgcgctcac cggcttcacc acggagatcg tgaagatcgc
    11941 gttatcgctg ctggaagccg atgggcagat caccattcaa cgggagcatg aagacaacct
    12001 gagcgttgcc agaggtaacc aaacagccga tatcgacgcc ctgcgcacct gtcaggaaaa
    12061 actggtgcat gccttcaagg tcatgcaggc tttccagcgc ttcttactgc gcctccctcc
    12121 ggaacagata ctttctgatt attatgaaag gggaaactaa atttatacct tttttatccg
    12181 atttattggt ataatgcgcc aaatgagcac catcagcagg agagattttc taaagaacat
    12241 gggagccgga gtggtaagcc tggctctgac cggacattca gaaaagtcga atgcccgctc
    12301 ttccacgcaa ctgggcaggg ttgcgaaaga cacatccatc agcgtgtaca gccaagccag
    12361 tgagaatagt gccattcgtt tccagcgcta tcgtgacgag atcttccata tttacgatga
    12421 ggtcgtcgct ccggatggtc cgggctataa ccccctctgg taccgtatct ggggcgggta
    12481 tgtgcatagc ggattcgttc aaaaagtgca gtatcgcttg aacacactca tcactgaatt
    12541 tcccagccag ggacaattga tggagttgac cgtgccctat tccgactctt atcgccatcg
    12601 catcaatgag aaatgggaaa aattttaccg gctctattac agttctacct actgggtttt
    12661 cagtgccaaa gaaggtcccg atggcatgct ctggtacgaa atccgcgatg ggctggttga
    12721 tctttcgtac tatgtccctg tggaacattt gcgcccggtt acggcagagg aactgacccc
    12781 tatccacgcc gatatagccc ccagtttgaa acgcatcgag atctccatcg atttccagaa
    12841 gttaatggcg tatgagggga gtcagttggt caaagagatg cgagtgtcga ccggcgtccc
    12901 ctatctcagc agcgctccca acaccatccc caccgatacc cccaaaggaa atttcaagat
    12961 ccatgccaaa cgtcccagcg tgcacatggg cgatggcacg ctgcgttcag atgctgaagc
    13021 atatgagctg cccggcgtgc cttgggtttc ctactttgag agttccaccg gggttgccat
    13081 ccatggtacg tattggcacc aaaatttcgg catggtcatg agccatggat gcattaatat
    13141 ggaatccgaa gacgccaaat gggtttaccg ctggacgaat gtgccagatg atgggaacag
    13201 cgaagggtat cgcactcccg tcttagtata ttaattcctt ttcactcccc atacatcata
    13261 ccgagcatta gcgaagcccc tcacccggtc ccacgctaac cagccaacaa tagggcaaca
    13321 caagggatct cattgtgtgc ccaaaaaccg accactcatt cagggaacct gaccctgtct
    13381 gctacgccat gctgaacctt tgccaggtct ctcacgcaat ccaatccaaa tcccattcta
    13441 accagccaaa caatagggta cccacaatcc tgtccatcac gtcatcccga gcaatgcgag
    13501 ggatctcacc gtgtgccaaa gaaaccagcc agtaagggtg gcaagccatt tccctcccct
    13561 acgtcatgca gagcgtagcc aagcatctcg ctctaagcaa aagtaacctg ccagcgatag
    13621 aaaactttat tattctggat aaacttccat gaaaacccaa aaagaaactc aaaccatcaa
    13681 aaaccgcagg aaaacttgat ttcccaccat ccttccgcgc tttgtcaatc gaatggctga
    13741 catgtcgcca ctcaccacct ccagcaatcc aatatcaacg agttcagcga tctgcttttc
    13801 atacacgcga aaaagagaga ttccgaacct ttcttgaaaa aggtcatcgc gcagcccctc
    13861 ctgcgtcagc cgcaacccca tcatggctgt ttcccccata tcgtcggcaa cggataaggc
    13921 tgtgttggtc tctaaccagg gaaatcctgc ttcttctcct gccgagtaaa gagctatacc
    13981 cttgacgtaa ggttggattc cattcaaatt tgcccagcgc cgagcgccaa aatggctatg
    14041 cgcagaagcg ccaaatccaa aataatcctg gttgcgccag tacagcaaat tatgctggca
    14101 gtgataattc accccatctt gttttgccca attggagatc tcgtattgct caaagccctg
    14161 tgtggcaagg aaatccgtcg cccattcgta catgtccgct gccagatcat cgtcgggagc
    14221 tggaagaagc cccgcatcca tatcgatcgc cagcggcgtt ccttcttcca ccgttaatgc
    14281 atatagcgaa agatgtgcag gctggagcag catggctttt ttcactgaat cctgccaatc
    14341 cttcatgctt tgatgaggaa gtccgaacat cagatcgagg ttgaaatttg tcatcccggc
    14401 ggaacggact tgttccaccg ccctgaccac atccgcgaaa cagtgcctgc gtccgagcat
    14461 ctgcaattcc ccctcctgtg cgctttgcat tccgatgctc atgcgcctca cgcccgcatg
    14521 ggtccactcc ctgcatttct ccatcgtgat atctgccggg ttggcttcca cagatatctc
    14581 cacatcggtc gtcacatgga aagaacgctt tatctcttga attaccctgg ggattgtgcc
    14641 agccgcaagg tacgatggcg ttcctccacc tgcataaatg gatcggattg catacaggct
    14701 ggcatatctc tccccataga tctttatttc tttctccaaa ctcgaaaaat attccggaat
    14761 gaaatgctcc attccggcgc tcgtgttaaa atcacaatac ctgcagcggc ttttacaaaa
    14821 cgggatatga acgtaaattc ctgcttcctt catgcgtaaa atctatcata tcattgatgt
    14881 tataatattt cggctttgac aacatataaa gagtgaagca aaatgaattc taaaaacaaa
    14941 ccatccacag agaaagtatg tcctacctgc ggaacccatt tgagcgtgaa cgctacacgc
    15001 tgcctggtct gcgggagaac gtttacggat gcacctgcat ccacccctgc caataaaata
    15061 aaaccgcgcg gtctgccgca gttcacggtc agtttgccca tcgcgttggg cgccgttctc
    15121 atcttgattg cgctcggcgc cggcatcatc tactttgtcc tgcgcggttc gggtcagatc
    15181 actgcagcgc agcctaccgc cacgctcacc ccaactccca ccctcgagca gagccccacc
    15241 cccaccctca ccgcaacgcc tgccccaacg gcaaccttgg aaccgcccat cgaatattcg
    15301 gtccaatctg gtgatacctg tagctcgatc gctcttcaat tcaacgttac cgtgaagagc
    15361 attgccgatc tcaacaacct ggcttccgat tgtggtgtgc tcaccctcaa ccagacactt
    15421 ctcatccctc gccccacacc taccccctca ccgcagccca cgggtaccct cagtgtggct
    15481 caaaaaaccg aatccgcctg caccaaaatc acctatacgg ttggcgcgaa cgacacattg
    15541 agcgccattg ccgccaacta caatgtttcg gtcgaaacgc tgaagactta caacggaaag
    15601 acctctgata atgtatattt gaacgaggta atcactatcc ctttgtgcga acgcctccca
    15661 accagcggtc ctacccccac tgccaccctg cccgcaccat ttgcggctac caacctgctg
    15721 ttgccagccg acggcgcagt tttcaacgcc acccatgaca ccgtcacgtt gcagtgggct
    15781 gccattggag gaatgctgga aggcgattcc tacgcggtga ccatcgaaga cctcaccgct
    15841 ggcgagggca agaaattgac cgattacacc aaagacacca agtacatcgt tccggttacg
    15901 ttccgccctg ccgaatccgc tccgcatgtc attcgctggt acatccaacc ggtgaggcag
    15961 tcgggaacca ccacaggtgg tgacccgatc tatgtgaccg ctggctaccc cagtgacccg
    16021 cgcgtcttca tctggtcggg gcttggaaca tcctctaatt aagaaagaga gcgggacatc
    16081 atgcccgctc ttctctttat tcctgagatt cgagaaaacg cgttgcttca atggcggctg
    16141 ccgcgcccat gccggcagaa atgaccacct gcctgtagcg tgaatctgcc acttcgcctg
    16201 ctgcgtacac gcccgcaacg cttgtctgca tgttctggtc gatgcggatg tatcccttct
    16261 catccagctt cagccaatcg cgaaacagtt ccgtgttagg gctatgtcct atgaaaacga
    16321 aaaccccacc gatcgccagt tcacgtgttt caccggtttt cacatctttc aattggagac
    16381 cgctgaccgc accttctcct ttgatctctt ctaccacagt atcccagatg aaggatatct
    16441 tgggattctc gcgcgcgcgt ttttgcagca acgcgcctgc tcgtagttca tcgcgtcggt
    16501 ggatgatcgt caccgaagat gcgtaacggg tcaggaacaa gccttcttcc agtgcgctgt
    16561 cgccgcctcc caccaccgct acctttttat ccttgaaaaa ccaaccatcg caggttgcgc
    16621 aataagacac accccgccct gtcagttcct tttcgcccgg aacgttcagt ttgttcgcgc
    16681 tggcacccgt ggtaacgatc aaaacctgag cgtaatgatc ctttccataa gtgctcacct
    16741 tgaaagggct tttagaaagg tctgctgcgg tgaccatatc atattcaatg cgcgctccaa
    16801 atttctcagc attcttggtg aagagctcag ccaactgccc tccctccacg ccatccggaa
    16861 aacctggata gttttcgatg acggaagtag tagctgcctg cccaccaact tccgttcctg
    16921 tgaaaagcag cggcttcaat tccgcacggg cggcgtaaag agccgcagcc aatcccgccg
    16981 gtccggaacc taagatgatc acactttcga tatctttttc gttattttcc ataatgataa
    17041 attccttcct tacagaacag tgtccgtggt cttcaacgct tcatccagta tagtgatgcc
    17101 cattgctaat tcttcttcat tcacgatcaa tggtgggatc accaggatga gattatcgtg
    17161 aacatagtga aataagcctt tggatagtgt gaacttctgt aattcattca tgattgtagg
    17221 agtttttcca tattctccaa ggggggtttc agatttctta tcttttatta attcaatggc
    17281 gccaaacagc ccgattccac gcacatcgcc caccgcagga tgcttttcct tcaatcggtt
    17341 gaaggctttc agcagtatgg gttgcatagc ctgagtatgg cggatcagtt tttgctcctg
    17401 cagcacgtcg atgttggcag tggctgcggc aaaggaaatg ggatgtccgt tataggtgag
    17461 cccgctgtcg aatttgcggc ggtcaaagta tgctgccact tcttcgcgca tccccaccgc
    17521 tcccagcgga gcataggcgg aggtcaaccc cttcgccatg gtgatgagat ctggcttcac
    17581 ctgccagtga tccaccgcga accaggcacc cgttctgcca aatccgctca tcacttcatc
    17641 cacgatcatc aatatgccat agtgatcgca caacgcacgg attccttgca ggtagccatc
    17701 tggcgggatg agaatgccgt tcgtgccggt gaccgcttcg aggaagatag cggcaatgga
    17761 atcagcccct tcatattgga tcagcttttc caagtattcc agatggcggg ctgcatattc
    17821 ttcatctgcc agttgcatgg catctggcac caggtcatcg cgatagcgat acgtagcagg
    17881 gaaatgcacc accccaccca tcacgttggg ttcccaagga acgcgccgca ggtctcccga
    17941 aagcgcaacc gctccatgag atgccccgtg atacgaccgg tagcgcgcca ggatcttgtg
    18001 tttcccggtg acagcacggg cgatcttgat ggcattttca ttggcatccg ctccccccat
    18061 ggtgaacaag aacctgctga tgccctgggg gactacctcg cataatttct ccagcagtcg
    18121 cgcccgcgct tcggtcgcca tgtgtggacc ggcaaaggtt agctcctgcg cttgtttgca
    18181 gatggcgtca atgacctgct gattgccatg accaatgttg gaacacatca ccatggagtt
    18241 gaaatcaaga taacgcttgc catcgaagtc ccaaaagaac accccttgcg cgcgctccac
    18301 cggaatgggc tggatctcct tttgcctgct ccaggtccag aaattatgcg ccatacagcg
    18361 atcgatcatt gcttggtttt ttccctgcgc cataggtaaa ctccctgtgc cgtttatttg
    18421 tatcctatca tacaatgcgg gggttaaaat agagagaaag gcaacggatt ttctgccaaa
    18481 cttccataag gagttttcta tgtgtgaaaa tactgcaatg atcttcgagg gaaagatcgc
    18541 cctgatcacc ggttccagcc gcggtatcgg gcaagccatc gccctgcact ttgcccgcct
    18601 cggtgcgcat gttgtcatca attacgtgca caatcaggag cccgctgaag aaacagcccg
    18661 gcagatccgt gccatgggtc gtaaagcgct ggtggtacgt gccaacgtcg gcaaaataga
    18721 acacctggag cgtctattcc aaaccgtaga acaggaattc ggagcattgg acatcttcat
    18781 cagcaatgcc gcgtcgggtt tcaaccgcac tgccctgcaa caacgccctg aaggctggga
    18841 gcataccatg aacgtaaatg ctcgcgcttt cctgtttggc acccaattgg ctgttccgct
    18901 catggaaaaa cgtgctgccg gtaagatcgt cgccattacc agtcagggtt ccgtgcgtgt
    18961 gatgcccgac tatgtctcgg taggcgccag caaggctgcg ctcgaaagct taacccgcta
    19021 tctggcggtt gagctggctc ccaagaatat tgccgtcaat gccgtttcgc ccggcgtggt
    19081 ggaaacggac gccctgcagc atttcgcttc gctttccgat cccaccgttc tccagcgcgc
    19141 catccaacat acccctgttg ggcgcattac cacccctcag gatgtcgctt gtatggtggc
    19201 atttctctgc tcggagcagg cgttcatgat ccgcgggcag gtcatcgtgg tagatggcgg
    19261 ttttagcctg cccgtgccgc actaatcgtg catcaacacc gcccgcaccg gagcgccttc
    19321 cactccctgt attttcatcg gcaggcatat cagcgtgtac gcacccggct ccacttccga
    19381 aaggtccagc gtttcgacaa tagcaatatg cttttccatc aaacgcgcat gtactggtct
    19441 gaaatcgtct gcgggcgaga tggaaagata atcaataccc accagttcga tcccctgctc
    19501 gatgagatac tctgcaccat ccaggctgag cgacacaaac tctgcgtgga aagtgtgggg
    19561 atcgtccttc cagtattggg aattgcgcgt cttgaaaagc acgcggcgta tccccgcacg
    19621 gattcccgcc ttttcgagaa catctctgcc aatgctgttc acctcgcacg gtatctgcac
    19681 cacctgcgcc tctccgatca acaggtcaag cggaaaatcc tccacgaggc ttccatcggc
    19741 taaaaaatgg cgcggagcgt ccaggtgtgt gccctggtgc aggctcaatt tcatttcgct
    19801 gaggttatag ggatccccct tggcaagctg caaaacctgc cgcacgatca tgggagggtc
    19861 accctcccag cgcaccattt cgggagatag ttcaatcgaa atgtctatga tcatagcttc
    19921 tactctcctg ctgaactgga ttgattttat aataatcgat aggaactgat aggtacgcaa
    19981 cttataatat cagaaactgg aaaattagga gcgagcatgc gcgaagtagc agccattggc
    20041 atcgggcaga cgaagatcga ggagcattgg gataaatcac tgaaagagat cgccggagaa
    20101 gccatttttg ccgccatcca ggattgcggt atcgatcatg tggatggtgt gttcgttggt
    20161 aacatgatgt cggcttcagc caacagccaa cagaaccttg gcgcgtatat caccgattgg
    20221 attggggcaa aacatgctgg tgcgcttcgt ctggaatccg cctgtggctc cggagctgcc
    20281 gcttttcgcg ccgcttttct ggctgtgact tcaggagaag cggaagtcgc cctggcagtg
    20341 ggagtagaaa aaatgaccga ttctcctacc gctgaaatca cctcccattt agccactgct
    20401 gccagtgccg attgggaagt ggatcagggg ctttcctttg tcgccctgaa cgccttgctc
    20461 atgcgccgtt acatgcatga aaccggttgg cagcataaag atttcgctcc tttttctatt
    20521 aatgcgcaca ccaacgccat gcacaatccc ttcgcccgtt tccatatgga actgaccgaa
    20581 caaaaatatc tcgaagccgg tatgatctgc gatcccatca atctcatgga tgcttccccc
    20641 ataggggatg gtgccgctgc catcgtgctc gttcccgctg ataaggtgag cgagaaggtg
    20701 attcgtgtgg tggcttctgc tgctgccacc gataccatcg ccgtccacga ccgcaaggat
    20761 cccacctggt tgagcgctgc agaggtctcc agcaaaaaag cctatgcgca gggtgggctc
    20821 gccccccaag atattgactt cttcgaatat catgatgcct tcaccatcat ggcagccctt
    20881 tctcttgaag cctgtggttt catcgagcgc gggaaaggcc cgctcatggc gttagaaggg
    20941 cacatcggca agaacggtcg cctcccggta gccaccatgg gtgggttgaa agcccgcggg
    21001 catccggtgg gcgccacggg cgcgtaccag ttggtagagg tgatccagca gttgcgcggc
    21061 actgccgggg aaaaccagct tgagaaagcg cgcgttggca tggcacaaaa catcggcggc
    21121 agcggctcca atatcatcac ccacatcctg cgcaatgact gacctggaca ctctgagaaa
    21181 gcgggtcata agctatcgcg aaaagaccta tcgcaccgca cggtcgttgc gcttgcattc
    21241 accccaagat gcgctcgatt tcttcaacga acgcggtttt atttttttct ggccgatcaa
    21301 ggattatgac ctgcccaacc tgtggtacgc caccgtaggg gccagacccg tccccaataa
    21361 ccatgacgat ccggggcacg tcacctgggg ctggaaggac ggcatgctcg acaaacgcgc
    21421 ctggtactat gccaaactgg tccgcaaaaa agccaccatt ctctcgctcg aaatggcgcc
    21481 ctatttttat gccctctcac ccaattatgg cgattatgag aatgattacc tgctgcagta
    21541 caagagcggt cagatgagcg tagagatgaa aaacgtgtac gaagccctgc tcgaaaatgg
    21601 acccttacac accctcgatc tgcacaaaca ggctcacctc acctcttccg acaagaaata
    21661 tctattcgat aaaacgctca ccgacctgca agccgaaatg aaaattatcc cggtgggcat
    21721 cgctgtcgcc ggtcgttggg gatatgccat gatctatgat atcgccgccc gttattatcc
    21781 agagatcgtg gaaaaagccc gcttcatcac ggaagccagc gcgcagcaag ccattcttct
    21841 ttcttatctg cgctcggtag gcatggcaga cctggttacc atcaagcgta tgtttacatg
    21901 gaaagaaccg ttcaccagcc aggcttgtgc gcaattggtg gaacaaaacc acatcaccgc
    21961 tatttttgat gccgaccgct ccaatcctct ctatttcatc aacgacattt ttcatctaca
    22021 ataataagac aatgaaatct ataccgcgtt cggcttcacc tgccagtgat catgcttcac
    22081 gcctgcctct cgctcacgct tcggagcagc cctcccctga agcgccctgg aaaaagaacg
    22141 tcgttctgct ggctaagaac gtcaacgtct ggctggcgca gttgagcggc aaataccgtc
    22201 agaagatcgt tcatctcgat cacatccccg ctgtggaatt ggaaaaactg gcagcctggg
    22261 gcatcaccgc cttgtggctc gtcggggtct gggaacgcag tcccgcttcc aggaagatca
    22321 aagaattgta tggtagagaa gatatcgccg cgtcagccta ttccataaaa gaataccgta
    22381 tcgcacgctc tctaggcggt gaaccagccg ccgacgccct gcacgccgcc tgtgatcggg
    22441 cgggcattcg cctgtgcgtg gatatcgtcc ccaaccacac cggcatagat tccgattggc
    22501 tgctcgagca tcctgaatgg tacatttctg tgtctgaaaa tcctgccgct tcatttcatt
    22561 tcaacagtcc tgacctttct cctactccta atatcagttt gcagttggaa gaggggtatt
    22621 atgaccaatc cgccgccgcc gaggttttcc tgtacaccga ccatcgcacg ggggagaaac
    22681 gcttcatcta tcacggcaat gatggctcga gcatgccctg gaatgatact gcccagctcg
    22741 attatttgaa tgccgaagtg cgcgaacatg tgatcgaaac catcttgtgc attctcaagc
    22801 gctttccgct gatccgcttc gatgcagcca tgaccctcac ccgccagcat ttccagcgct
    22861 tgtggtatcc cttgcccggc agtcaggaac gctgtgtgcc cacccgtgaa agacacgccc
    22921 tgccggcgga agccttcttg aaagccaagc ctcgcgaatt ctggcgcgag gtggtggagc
    22981 gggtggagcg cgaaaacccc gatgcgctac tcatggctga agctttctgg tatatggaaa
    23041 actttttcgt cgaagaactg ggcatgcacc gcgtttacaa tatcgctttc atgcaatcct
    23101 tgcgcgatga agataataaa acctatcaga gcattttgaa aaatgcgctg gcttccaacc
    23161 ccctgctcct cggaaaattc gtgaactttc tcagcaaccc agatgaacgg actgccctcg
    23221 accagtttgg gaaaggcgat aaatactttg ccgcctgcac gctgctggtc accatgccag
    23281 gtattcccat gttcaatcat ggacagattg aagggttctc tgaacgctac ggtatggatt
    23341 ttttgcttcc cctgctgcat gaaaaagagg atgccgattt gctccgcctg cacgaacgat
    23401 ggatcttccc cctcttgcgc atgcgtccct gcttttccga tgcggcttcc ttctgcctgt
    23461 tcgatgtcgt aggtcaggct cagcgttctc tcccccacat ctatgcctat ttaaaccatt
    23521 atcacgaccg ctacttcctg gtgctggtca acaactcatc ccgcaaccag gccatccgct
    23581 tccggcgcac cgtgccgctt cgtggaacct ctggaaagcc tgccgtttct acacttctgg
    23641 aatggctgcc gcgttccgct gacccacaag ccaatctgcg ctgccgcgag gtgcgcaccg
    23701 gcgaggaatg gtcgtttgct tatcaagatc tggaagagac cgggatttct cttaaactta
    23761 aaccctacca gcaccttgtc tgggagcttt tctggagcga accaccgcta catagcgctc
    23821 catcttctcg ctgaaacgcc ctggtaagat gttgaattgg tgcggcagcg aatcgagtga
    23881 gcaggtgcga tcagaagcca agtaatcgat ccagaaagtg gaaagggtga aaccgcttgg
    23941 cgattccccg aaccataagt ttctggcgtg cagcgccgct ggggcaatag gaaccaggta
    24001 ccgtttcttg ccgatccttt ccatgatcat ttgcaggatg gctcggaaag tgaagtactc
    24061 tcccccaccc agctcataaa tcttgcccgc atagtcctcg ttttgatacg ccaggtacag
    24121 gcagttgatc agatcattga tccacagtgg ctgcatgaga atgtcatcgc tgcccggaat
    24181 ggcatagatg aacttgctgg ctgccagccc gcgcgcaaaa tacgaaagaa atccatcctg
    24241 ttctccaaaa acgaccgatg ttctgaagat ggtggcggtc gctttgctgt tttgcagcat
    24301 cttttcttga acagctttgg cacggaatgc aggaaacccc gatgtgcgct ccgccccgat
    24361 atggctcagg tagaaaatgc gctccacccc cacctgctca gcggctgtca gcaggttctc
    24421 caatcccggg atatcgcttt tttcaaggtc gatgtgctga ccttcctttt ccgaacttgc
    24481 caggtgaaag atgcagttta catctttcaa tgcagccctg atgcctcgtt catcctgcat
    24541 gctgctcacc gccacttcca cgggaatgct gtgcggcagt tgtggcgaat a
//
LOCUS       DAOSDS010000025        24537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_911_length_24537_cov_5.548689, whole genome shotgun sequence.
ACCESSION   DAOSDS010000025 DAOSDS010000000
VERSION     DAOSDS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24537
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_24537_cov_5.548689"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="PLL88_04635"
     CDS             <1..187
                     /locus_tag="PLL88_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00883.1"
                     /translation="DSHGDKLIAEQLAARPGWDQVDAVKNNRIYLIDGNISSRPGPRI
                     VDALESIAKSLYPDLFN"
     gene            274..1299
                     /locus_tag="PLL88_04640"
     CDS             274..1299
                     /locus_tag="PLL88_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOJ00884.1"
                     /translation="MSHRRFTLYLLLLMGILLATLVFSLGVGAVSIPPRRVLNVILNS
                     AQVDRDSTDIAIVRDLRLARVLLAALVGAGLATAGAAFQALFRNPLADPYIIGASSGA
                     SLGATLAITLGLSWGFAGFGPVPLAAFLGALLAVFLVYIIAGSSGNSTVIGMLLAGAA
                     LSTVLSSAVSMLMLISNESIHEIFAWLMGGFSGRSWIQFWSSGPYLVIGMALLWLCSR
                     PLDALSCGDDTAQTIGLSLRKARGGIVAAASLVTAAAVAASGIIGFVGLISPHIARML
                     FGATHKRLIPTSALIGAILTLFADDLARTIMAPLEIPVGIITSLVGGVFFIYLLKTRE
                     QGTRGGP"
     gene            1296..2114
                     /locus_tag="PLL88_04645"
     CDS             1296..2114
                     /locus_tag="PLL88_04645"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HOJ00885.1"
                     /translation="MTSTLELVNVTCAYNREPVVRHISLQAHPGEVLALIGPNGVGKS
                     TLLRTMARLMRPVDGSVILVNRDLWQTSAREIAKQLAFAAQSNDVTWAATVEQVVALG
                     RAPHRGWFLPLTAHDRQIVRKAIELVGLEHLRDRISTKLSGGEQQRMVLARVLAQEPS
                     ILLLDEPTSHLDLKYQTDILSLIRRLAHQEGLTVIVSLHDLNLAAIYADRVALLSDGE
                     LAVAGTPTEVLTSDWLSQVYGVPILVMKHPLRDIPFILPNIDLSEKVENDTESK"
     gene            2095..2643
                     /gene="cobO"
                     /locus_tag="PLL88_04650"
     CDS             2095..2643
                     /gene="cobO"
                     /locus_tag="PLL88_04650"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOJ00886.1"
                     /translation="MTPNPSNTRNRKGLVIVNTGDGKGKTTAALGVILRSWGRGFRIC
                     VIQFIKPAHFNTGEVLAAEKMGIEWHRCGDGFTWLSKDEAQSSALAHSGWQLAQEKIS
                     SGAYDLVVLDEFTYPLHFHWLDTDEVVNWLSVNKPAHTHLIITGRSAPPKIIDYADLV
                     TEMKLIKHPFNQGTKGQFGIEF"
     gene            2674..2877
                     /locus_tag="PLL88_04655"
     CDS             2674..2877
                     /locus_tag="PLL88_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00887.1"
                     /translation="MSVEQSTLLEFVGLTAAQSFTEISAYAFHQSATLIGGGVSCCDC
                     SESETIFNDCESYHDLGKHVLGG"
     gene            2828..4321
                     /locus_tag="PLL88_04660"
     CDS             2828..4321
                     /locus_tag="PLL88_04660"
                     /inference="COORDINATES: protein motif:HMM:NF001989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HOJ00888.1"
                     /translation="MTAKAIMILGSMSSVGKSLLASGLCRLYARKGWKVAPFKAQNMS
                     NNAAVCSGGEIGRAQAAQAHACGIEPTIDMNPVLLKPEADSRSQVIVRGQVWDSLSAK
                     DYYARRATLWQAITASYDSLSSEFDLIVIEGAGSAAEINLKAHDMVNLAIARYAHAPC
                     LLVGDIDRGGVFAQLLGTLWLLDDAERNLIKGLIVNKFRGDLNLFLDGVNAIEARGGV
                     PVLGVVPYIHDHGLAEEDAATLVERYSSCSEKADIAVVHFPHISNFDDFDPLLAEPHV
                     QMRYVNRASQLGKPAAIILPGTKNTLSDLRWLHESGLSVRIQSLAAEGVVVVGLCGGF
                     QMMGGLVTDENGAESAIRSAPGLGLLPGNTHFELAKTVTRSRAHVFASGGFMEKIQGQ
                     IIQGYEIHMGQTTSPRPFCHIFQRESSSVDSQDGACSEDGSIWGTYLHGVFDNDLLRI
                     AWLESLKVSASTVPFAERRNKTFDLLADALEASLDVKRLDRIIAEGI"
     gene            4323..5261
                     /gene="cbiB"
                     /locus_tag="PLL88_04665"
     CDS             4323..5261
                     /gene="cbiB"
                     /locus_tag="PLL88_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HOJ00889.1"
                     /translation="MQTALIGLAVAFFIDLLFGDPPNRFHPVVAMGNFIRHIQKRYNT
                     GSQKERFLAGLGTVVFGGALFSLPWLVILPLLTKLPIWAAGALMSCFLKPVFSFRNLI
                     RAGKEIRQYLLINDLSTSRRMVGWHLVSRDTSQLSKNQLASAVIESLAENITDSFWAP
                     LLFFSIGGLPAAWFYRFVNTADSMIAYRTPDLEHFGKSAAKLDDFLNWLPARAAGIIL
                     VVSAGLLHLNMKRAWHTMRTQNDRTSSPNAGWTMAAAAGALEIVLEKPQHYRLEGGTQ
                     LPDEKSIERSIYLVSLALVLSLFICGGIIFVVHSIS"
     gene            5242..6384
                     /locus_tag="PLL88_04670"
     CDS             5242..6384
                     /locus_tag="PLL88_04670"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOJ00890.1"
                     /translation="MSIQSHEEHGGVDPAELKRHGLCIGSLIDFSVNSNPFGPSPCVV
                     DALKHVGISRYPDRNCSELAGRLAELNHVSEKRILIGNGTTELIWLICHAFLRPGDRV
                     LIIGPTFSEYHRASIRLDASVTEMSAMPPYFVPPLNKAIDFIREFHPRLVFLCNPNNP
                     TGKYVPDGEIQDLIAVCGFESILVIDKAYSAFLDGSFFELTIPQNVLILRSMTKDFAL
                     AGLRVGYLLGDADSIEKTKAFQPTWSVNAYAQAAGLAVLSDPEYYRNSLALLKRIQVS
                     FFDEIRSIGYRLITSDVHFALVHCGRPANLFRSQLLRSGLQVRDCTSFGLPHYIRICT
                     RLQEENKKLIMAMKAIETIDPGRNDVQANLPRKDATDDDTSSDPTL"
     gene            6356..7369
                     /gene="cobT"
                     /locus_tag="PLL88_04675"
     CDS             6356..7369
                     /gene="cobT"
                     /locus_tag="PLL88_04675"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOJ00891.1"
                     /translation="MILPAIPLFNEKSAENARIRQQRLAKPIGSLGMLEALSIQLAGL
                     TGNEHPRFHQKEVIIMAADHGVAQEGVSAYPSDVTAKMVKNFLTGGAAVTVLARQANA
                     SVTVVDIGVNDDLEDVPGLLHRKIMRGTFNMLREPAMTRAQAEEAIQVGIDVVDQRVK
                     GGMDLVATGEMGIGNTTPSSAIAAVYTKLPVERVTGRGTGLDDAGLTLKIKVICEAIA
                     LHQPNIQDPLDVLAKIGGLEIAGLTGVILGAASHRIPVVVDGFISGAAALIAVEMLPD
                     VKPFLIASHQSEEVGHTAILQKLGLKPLLNLELRLGEGSGAVLAFHLVEAATGILNEM
                     ATL"
     gene            7394..8119
                     /gene="cobS"
                     /locus_tag="PLL88_04680"
     CDS             7394..8119
                     /gene="cobS"
                     /locus_tag="PLL88_04680"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOJ00892.1"
                     /translation="MKYFAVAISFLTVFPVKVKEPILPGDLGKSSSWFPLIGVFLGGL
                     TAGIFFVCSIFLPPLVTAVLTIAIWIGLTGGLHLDGLADCCDGMWNASNPERRLEIMK
                     DPRLGSFGGIGLILCILIKVACLNSLPSGVFWTAIPLAVSTARWLLLCAGKQKMARST
                     GMGADFSSGITPRSIIPALLTILMATILSGWKGILIVAFTHGVAWLVARVAIKQLGGL
                     TGDVFGLIVELSECIVLLGFSVG"
     gene            8129..8935
                     /gene="cbiR"
                     /locus_tag="PLL88_04685"
     CDS             8129..8935
                     /gene="cbiR"
                     /locus_tag="PLL88_04685"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HOJ00893.1"
                     /translation="MKKIKKAPFRIGTTSYIIQDEILPNVRFLADLVDDIELVLFEVD
                     DGPNNLPDQKTVDELIQIAQQHHLSYTVHLPLDLRLAGDDGVQHISLVKARKVITCTR
                     ELHPHAYVLHLDGKDILHRNDPISLARWNRQALLALQQVSAWVENSEMLAVENLEHYP
                     PGLWDEVIKRASVSRCVDVGHLWVDKLDPINFLAERIGETRVMHIHGIRERDHQSLIN
                     VPADELERVLDFVIQSNFQGVMTMEVFSEDDFRSSMETVEKVLEKLELED"
     gene            8935..9477
                     /gene="cobU"
                     /locus_tag="PLL88_04690"
     CDS             8935..9477
                     /gene="cobU"
                     /locus_tag="PLL88_04690"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOJ00894.1"
                     /translation="MGQQLTLIMGGARSGKSDYAQFLVQEDEQPALFVATATAGDEEM
                     ANRIAVHRANRPENWQTLEAPLNIGTAIQNAPPFPWVLVDCITLLVSNLLMTCAEPVD
                     ERGYHAMVEREMDELIASYSIHTGKWVVVSNEVGLGLVPAYPLGRYYRDALGRANQRI
                     AKIADRAILMVAGIPMIVKE"
     gene            9693..9860
                     /locus_tag="PLL88_04695"
     CDS             9693..9860
                     /locus_tag="PLL88_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00895.1"
                     /translation="MASSRICVRQRDGFILDEPTFAFDAGTEFETLRQMLNDKVSKVA
                     FLVFHRFLTVG"
     gene            9937..10695
                     /locus_tag="PLL88_04700"
     CDS             9937..10695
                     /locus_tag="PLL88_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOJ00896.1"
                     /translation="MHTKAILYESQERVCTFECDFQRRWKPAAMFQHLTEAATSHSEQ
                     LGAGFDVMLSQNLFWVHSRMKLLFVAFPKAGDEIIIRTWPKTIQQKLFYIRDFEVLTS
                     SGQPLALATSAWLVIDATRRRMIPPRSLDIDLPSLPDKSGLDEPLEKLGLDDDGEERL
                     RVRAGYSVVDMLGHVNNSRYIEWICDAFPMEMYERNTLDWIQINYDREVRPGEEVGLL
                     VKEQAGENAVWQVVGNNHSNGTLAFEALLHWKGQ"
     gene            10866..12350
                     /locus_tag="PLL88_04705"
     CDS             10866..12350
                     /locus_tag="PLL88_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00897.1"
                     /translation="MKQLSNASSQRLVKESSESSYIRKILIAIYFGSLFVHFLCVFFF
                     INQPIALDDMYQYDMLGRSIENGNGYRWYSKTDVDVLKPYYSQFLDLDNLDFPENGIL
                     TTFRAPGYPFFLALIYSLVPSSLRFVFVRIAQACLMAILTPISAYIAYKLKCKPKTIV
                     GAGIFSSFYPILLFYPIGLVSENLYIVLGTSSLIALMVSIENKKKEGWILLAALLCGL
                     TMLTRSIYAMYVLLAGFWIVGYRQVKWKTGLLFMRVAFGICMPWAIRNSIVLGSPSFV
                     ENSLGYNLFIGYHPEGNGGFVSEIAIQPLNILDDGERNIFCFEKALNFIRQDPVETVR
                     RFFIRVGMFVGPEIREFTFFYTNDFLGAIPTFFLVLIYLVLTIPWLTLVILSPVGFWY
                     AREKGFVVLALIFILGYSIPHFLILAEPRFHLGIVPVLIPFAGYAFENRKEIKNYHFR
                     FQKDRLFLLIYLTILICFGITLFHNLNDIKDVFNAGGNTLRYSY"
     gene            12638..14269
                     /locus_tag="PLL88_04710"
     CDS             12638..14269
                     /locus_tag="PLL88_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOJ00898.1"
                     /translation="MNSFDDFSEREQAVIKLLVQGKSNKQIALQLKISNRTVEFHLSN
                     IYSKLGVSSRTEAVLLFMKDLSGEQAEEPVEITWRESTDAPMDEPSYNGEKREQRNPM
                     KKRTWYLIGSGLLIAVFLALVFFIDPEHNLKNFFTRIIEGDSGIISVEVAAPQEDGSI
                     IHTVEEGQTLWSIAVAYDVPVNQLRFLNQISEGSDVIYVGQQLYIRTASTQIPTSTPI
                     AAENTFTQTLDSSRVTLTVKWFYIDQTRLYLDLVVSGYPLPEDFSPLQLIDLQNVVIH
                     RSDGSLIDLEHSIRYGGIGGGGGENSQDQEPQFFEGIIDVGLPSSPQENSQEEPYVMD
                     ILIGGEIYAENGEVRVVLPVVPFHIEVKPTYVGQLTFAADESAVIDEKTVTFKGMEIN
                     PSNAAVLFCVFDPQGSQWLPSIHLLYKGNVLDEAGLALTDGDPSSQMCYRIYYTRSFQ
                     FDPKNDPRTNIAILVAKLVKDQPERLPYELVAVVQNDLAAQGIEFNYVIISHGSGIEV
                     TKRPAGMTEEEAQLIIHNALVAEAIPEGTLIFHLP"
     gene            14313..15038
                     /locus_tag="PLL88_04715"
     CDS             14313..15038
                     /locus_tag="PLL88_04715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ00899.1"
                     /translation="MELPIQNIRILVIEDDRAVARSLHDALVKEGYSVQLCDSGGKGF
                     EAAQQNQPHLIILDIRLPDGLGFDFCKQMRKAGLYQPILVLTVRDEPIDKVLALEIGA
                     DDYMTKPYNPQELHARIRALLRRAYGEFSSTESKLVFVGDLIIDVLKATVSRNQTVIN
                     LTPTEYRLFVHMAKHPGQVFSRSQLSNSIRNCDNEEVDDSQTITVYIRRLREKLEYDP
                     AHPKLILTVPGIGYRLSLSPETH"
     gene            15253..16101
                     /locus_tag="PLL88_04720"
     CDS             15253..16101
                     /locus_tag="PLL88_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00900.1"
                     /translation="MKKFVLSIFMVLALLLAACGGSATENSDVQDLAAPAVATTSETP
                     AVSDNGPAQPSGDRGAVADGEVSELTRLLIGIFQLESTDLALTTDQAAALIPMLNSYL
                     ELEQSQNLASRQQGQQAATPGTSTGAMESQQATMQAQQIVLQEQQSALIVQIETLLSS
                     DQLTFITSQELDQEAVRAFLEEQGLSAGGGNPGSGGQGSEQSISQGTPAVDQPGNVPQ
                     VQGTPGNGNGGPGGNLQDGSAPASMTPGADQGFGQPNQGGGGLGSTSRILIDALLQLL
                     ETKMSD"
     gene            16199..17164
                     /locus_tag="PLL88_04725"
     CDS             16199..17164
                     /locus_tag="PLL88_04725"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HOJ00901.1"
                     /translation="MKYWFATLTPSLPATFYAMEINVKFKMTIPDEIKKERRIAISIN
                     LRRVGQMVTHPRQVFAELENENDKADWKSPIIVIGLILILASLMSVTSSSGTSATQAQ
                     TYGSNTSTGFSNMSPGGMQGGGPMGMGGQTQSTATTDQSSATDETATSTTASSSTLLS
                     KILSAAGALLSFVLTWLLVGSLANLISISLGGQGNTKLATVFSAWACVPIGIRSLMQI
                     MYVLATGNSIQAVGLSGFAPTSSTTLATLLEKILAQIDIYSIWQTVLLVIGMGVMTKL
                     DKKKSIVIAVTSIVIVVLLKSLLGLGVEKIASINVSNGLLNTMMR"
     gene            17161..17832
                     /locus_tag="PLL88_04730"
     CDS             17161..17832
                     /locus_tag="PLL88_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ00902.1"
                     /translation="MTNIIQVQALSKVYEMDDDVQVIALAGVNLEIEEGSFVAAMGPS
                     GSGKSTLMHIVGGLDRPTSGSVEVGTYHVTTLDEQQLAFFRRKMIGFIFQSFNLIATM
                     TALENVALPLRLCGIPRSERLERAYHLLEKVGLKDRVYHKPTQLSGGQQQRIAVARAL
                     ANDPPLILADEPTGNLDTASGENIMVMLQELNKNGRTVMVVTHDPRITKYVNRVINML
                     DGHLQ"
     gene            17856..19358
                     /locus_tag="PLL88_04735"
     CDS             17856..19358
                     /locus_tag="PLL88_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00903.1"
                     /translation="MKKHLIFILLIGMLIFSGFTFSASFKDTSNGKAVLQEETTEATE
                     TVAATATTEVAATATVAPTSTTVPTATTAAAYSRPLLNLTSYSYDSSEVYAGNVFSLD
                     CVFQNLGQQRAYNVVITYSANSLTAENNGGVDVISSLGVAESATSEQDFYVSSSISSS
                     PITVNISIEYRDQDGKSYTQSFAVSVSVTGYGTYYTSTPEGRPQMVVASYETDVDPLE
                     PGTSFVLTMNLQNRGYLTAQNVSMVFGGTADSSSTTSSATSSSTNFFPLGSSNVQVIG
                     NVGIQGYVQVQQTLVVNSETTPGVYPLTLSFTYTDEDGNEFTDSQIITLLVHLVPSIE
                     ISFYETPDVFYVGEEDTLPIQVVNIGSDSILLGDIYINVENATLSNNLTFVGTLESGG
                     SFTIDTDIIPQQAGSLPVTVTINYLDNFKKAQTITQTLSITVSDTQMPAATIDPALVA
                     TEMARMPAGGNQTSSDLGSILLRFLRGMIGFDSSASTGQNRSNPGLMNTN"
     gene            19380..20735
                     /locus_tag="PLL88_04740"
     CDS             19380..20735
                     /locus_tag="PLL88_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ00904.1"
                     /translation="MSFIELAKFIFENIKRQKGRVILTSLGVMIGSASIVLLVALVGG
                     LQQSATAQFTSNASLSEITVSAGFSGGFSMMGGGGSPGGGGGMGGGGSTVRTSSESEE
                     LLLTDDVVETISFLEHVASVSAEQSVSGNSTLIYQKYEGRVSLTGVDVDDLSELGLSV
                     DEGETELNSGTIILGYSVSENMYRPNYSGSDATLTTNLLGKQVKVEITKMSSSGRSVK
                     TMRFRVVGILAESGDQSDYAAYITMTDADTIVSWVNGQRVNHSTDGYSSLTVRVDDSD
                     NVLAVADEITAMGYQTQTAQSFLENVNSLFSSLQLVFGGVGGITLLVAALGIANTMTM
                     AILERTKEIGLLKALGATNKDILGLFIGEAAGIGLIGGMGGLVISMLLGSVINAVGGS
                     YLTEQATASGTTVTTSILYMPFYLPLFALAFSTIMGSLSGLFPAMKAQSLPPVIALKY
                     E"
     gene            20737..22317
                     /locus_tag="PLL88_04745"
     CDS             20737..22317
                     /locus_tag="PLL88_04745"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ00905.1"
                     /translation="MKKEKRRLFNSLRAQLPLSYAGIALITTTLIGAVLLFIIWNYYH
                     GLEKQYLESNLHGTANNLTRIVEENDITATTSLIDYSDVFQNQANITAFLIQSRMRVL
                     DTNGNIIADSGSPTSSWNITISKPKNSDNQQRVQNEGENDERPSFSLEKSAGQPSQDS
                     EGNPPPYSIQANPHMFGYLLEDSMNEEGKRSSVVAKEAFYDKDENILGYVELSEGPGY
                     GQRIIFNVLRGWAIASLIGLIVSVTFGWIMSRKLTKPLVELEKVAVEMKKGNYEIRSP
                     ILKPNELASLSGTFNQMAAHIQQSIETLRQFISDAAHEIRTPLTSLRADLTLALSENS
                     RKGLKPIVKRSLEQVDRLDQLSRDLLDLSKLESRNTVAPVEEVNLADLLTELSEVHAS
                     AAEQAGITFQFQLPADALIVRGDKGQLYRAVGNLLDNAVKFTPVGGRVVLSLTARNNF
                     AQITVEDSGIGIPADERELLFKRFHRGKNTQRFPGSGLGLAIAKAIVEKHQGEIGLMP
                     DADRTIFFIRVPLLVHKK"
     gene            22404..23396
                     /locus_tag="PLL88_04750"
     CDS             22404..23396
                     /locus_tag="PLL88_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ00906.1"
                     /translation="MEFQIFTPHHVDDAATLLVAQSTSMLKRFPLLPERVGNLHAAKI
                     FLSALLEKSEARGMLMLEKNKVVGYLLGYYDDNPFFGRHVWVPFGGTVVPQSQAYGAI
                     RALYAAAGDIWIGDGILNHYWVCPALDDWLKAGFSLSFGQEQVYAVAPLKEREAGTAL
                     PAGITMRVVQPGDAQQLAQKGHWIAAHLNGAPVWEPVPPEHLENIRPAYAELANDDTS
                     TTWVALDGEEIVSYVTIYTVDIGAIHYLGSQKAAHFATAATDPAYRARGIGRALATQI
                     FNIAGEQGYETMFTDWRITNLEAARYWPTFGFEPFAYRLLRRVNPRYQRFEMPK"
     gene            complement(23402..24298)
                     /locus_tag="PLL88_04755"
     CDS             complement(23402..24298)
                     /locus_tag="PLL88_04755"
                     /inference="COORDINATES: protein motif:HMM:NF015500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HOJ00907.1"
                     /translation="MRQFVGEISSYAHSLDPDFLVIPQNGQELLVQGDDVNSEADRAY
                     IASIDGIGQEDLFYGFEADDLATDPTVTSLWIAYLDLARQNGLAVLVIDYCSTPEKMD
                     ASYQRNHELSCLSLAADRRELDRIPSYPPGPFSENDDTIETLGQAQNFLYLINPARYS
                     SKEDFIRAVTATNYDLLIMDLYLGDEAFTRDEVEQLRNKANGGSRLVLCYMSIGEAEA
                     YRPYWKAEWDSNPPEWLLEENPNWAGNYRVQYWNKEWKALITGSADSYVGKIIHSGFD
                     GVYLDLIDAFSELEDEASLDNE"
BASE COUNT         6203 a         5589 c         6343 g         6402 t
ORIGIN      
        1 cgactctcat ggtgataaat taatcgctga gcagttggca gcgcgccccg gttgggatca
       61 ggtcgatgca gttaagaata atcgcatcta cttgattgat ggcaacatat cttcccgccc
      121 tggtccacgc attgttgatg ctttggaatc aatcgcgaaa tcgctctatc ctgatttgtt
      181 taactaattg aatctgacag ttttgccgca gagtcatcat ccctctgggg tttttgactc
      241 tgcggtcaat cgttcacaca aagttgcaat gcaatgagtc atcgtcgatt taccctctat
      301 ttgctgctct tgatgggcat ccttctcgca acgctcgttt tcagcctggg cgtgggggct
      361 gtttcgatcc cgccgcgacg cgttttgaat gtcatcttga attcagcaca ggtcgatcgg
      421 gattcaacag acattgctat tgtgcgcgac ctgcgcctgg cgcgcgtttt acttgcggcg
      481 ttagttggtg ccgggttagc aacagcagga gccgcttttc aggcattgtt ccgcaatccg
      541 ttggcagatc cgtacatcat cggcgcatcg agcggagctt ctttaggcgc gaccctggca
      601 ataacactgg ggctatcatg gggtttcgcg ggttttggtc cagttccgct ggcggcattc
      661 ctcggggcgt tgttggctgt ttttcttgtc tatatcatcg ccggaagcag tggtaattcg
      721 acagtcattg gcatgctttt ggctggagcc gccctcagca ccgtcctttc atctgcggta
      781 tctatgttga tgttgatcag taatgagagc atccatgaaa tttttgcctg gttgatgggt
      841 gggtttagcg gtcggagctg gattcagttc tggagcagcg gaccctattt ggtgatcggg
      901 atggcgctct tatggttgtg ttcccgtccg ttagatgctc tctcgtgcgg agatgacacg
      961 gctcaaacga ttggcttgtc tttgcgaaaa gcgcgcggag ggattgtagc cgccgcaagt
     1021 ctggtcaccg cggcagcggt ggctgcaagc ggcatcatcg gttttgtcgg cttgatttca
     1081 cctcatatcg ctcgcatgct ttttggcgct actcacaaac gcctcatccc taccagcgcg
     1141 ttaattggtg caattcttac gctgttcgcc gatgacctgg cacgaaccat catggctcct
     1201 ctggaaatac cggttggcat tattacatca ttggtgggtg gtgttttctt catctatctg
     1261 cttaaaacac gtgagcaagg aacgagaggt ggaccatgac gagcacgctg gaattggtca
     1321 acgtcacatg tgcttataac agagaaccag tcgtccgcca catatctttg caggcacacc
     1381 ctggcgaagt attggctttg atcggcccta atggggtcgg taaaagcacg cttttgcgga
     1441 caatggcgcg cttgatgcgc cctgtggatg gtagcgtgat cctcgtcaac cgcgatcttt
     1501 ggcaaacttc tgcgcgtgaa attgccaaac aattggcttt cgctgcccag tcaaacgatg
     1561 tgacctgggc tgccactgtg gaacaggtgg tcgccttggg gcgcgcccct catcgaggtt
     1621 ggtttttacc attgacagcc catgatcgtc aaatcgtgcg aaaagcgatc gaactggtag
     1681 gtttggagca tctgcgcgac agaatttcaa cgaaactttc aggcggtgag cagcagcgta
     1741 tggtgctggc tcgagtgctc gctcaagaac catccattct tctgttggat gaaccaacct
     1801 cgcacctcga tttgaaatac cagacggaca tcttgagcct catccgtcgc ctggcacacc
     1861 aagaaggact gacggtgatt gtaagtttgc acgatctgaa ccttgccgcc atctacgccg
     1921 atcgggtggc attgttaagt gatggggaac tcgctgtggc aggaactccg acagaagtgc
     1981 tgacttccga ttggctttca caagtctatg gcgtgccgat tctggtgatg aaacatccac
     2041 tacgggatat tcctttcatc ctgccgaata tcgatctatc agagaaagta gagaatgaca
     2101 ccgaatccaa gtaacacgcg aaatcgaaag gggctcgtca tcgtcaacac tggtgatgga
     2161 aaagggaaga caacggctgc tttaggtgtc atccttcgtt catggggacg aggattccgc
     2221 atttgtgtga tccaatttat caaaccagcc catttcaata cgggtgaggt tcttgccgca
     2281 gagaaaatgg gaatcgaatg gcaccgttgt ggcgatggat ttacatggct atcaaaagac
     2341 gaagcgcagt cttctgcgct tgcccacagc ggttggcagt tggcgcagga aaaaatttca
     2401 agcggggcat atgatctggt ggtgttggat gaatttacct atcccctgca ttttcattgg
     2461 cttgacaccg acgaggtggt gaactggctc tcggtcaaca aacctgcgca tacacatttg
     2521 atcatcacgg gcagaagtgc acccccaaaa atcattgatt atgccgacct tgtaaccgaa
     2581 atgaaattga ttaagcaccc cttcaatcaa ggtaccaaag gacagttcgg gatcgaattc
     2641 tgatgcgttc agtgattgga ggtgtcaata gtcatgtcag tagaacaatc gacgctgctg
     2701 gaatttgttg ggcttacagc agcacaatcg tttacggaga tttctgcgta tgctttccat
     2761 cagtccgcca cgctgatcgg tggaggggta tcatgttgcg attgctcaga aagcgagacc
     2821 atcttcaatg actgcgaaag ctatcatgat cttgggaagc atgtcctcgg tgggtaaaag
     2881 cctgctcgcc agtgggcttt gccgcttgta tgccagaaaa ggttggaagg tggcgccttt
     2941 taaagctcaa aatatgagca acaatgcggc ggtgtgttcg ggtggagaaa tcggtcgggc
     3001 acaagcggct caggcacatg cttgcggaat agagcccaca attgatatga acccggttct
     3061 gttaaaacca gaagcggatt cacgcagtca ggttattgtg cggggacagg tatgggattc
     3121 actctcagcg aaggattatt acgcgcggcg cgctactttg tggcaggcaa ttacagcgag
     3181 ttatgacagc ttgagcagtg agttcgattt gatcgtcatt gaaggagccg gcagcgctgc
     3241 cgaaatcaac ctcaaagcgc acgatatggt caacctggct attgcccgct acgctcatgc
     3301 accatgtttg ctggtgggag atattgatcg cggcggagtc tttgcgcaat tattggggac
     3361 gttatggctc ctggacgacg ctgagcgaaa tttgatcaaa ggattgatcg tcaacaagtt
     3421 tcgcggagat ttgaacctgt ttttagacgg agtcaatgcc attgaggccc gcgggggtgt
     3481 gcccgtgtta ggcgttgtgc cttatatcca cgatcatgga ttggcagaag aggatgccgc
     3541 gacacttgtt gaacgttact cgtcttgttc agaaaaagca gatattgcgg tggttcattt
     3601 tccgcacata agcaattttg atgattttga tcctctgctt gccgaacccc atgtgcagat
     3661 gcgatatgta aaccgtgcaa gtcaattagg aaagccggca gcaataatct tgcccggcac
     3721 aaaaaataca ttgtcggatt taagatggtt gcacgaatct ggactgtcgg ttcgtatcca
     3781 gtcactggca gccgaaggcg ttgtggttgt cggcttgtgt ggcggttttc aaatgatggg
     3841 aggactggtt acggatgaaa acggggcaga atcagccata agaagcgctc ccggtctggg
     3901 gctgctgcct ggcaataccc attttgaact ggcaaaaacg gtcacgcgca gccgggcgca
     3961 cgtgtttgcc tcgggaggat tcatggaaaa gatccaaggg cagatcatcc agggatatga
     4021 aattcacatg gggcaaacga ccagtcctcg cccgttctgt catatttttc aaagagaatc
     4081 gtcttctgtt gattctcaag atggcgcatg ttcagaagat ggcagcattt ggggcactta
     4141 cctgcatggc gtttttgata acgacttgct gcgcattgcc tggctggaaa gtttgaaagt
     4201 cagcgcttca acggtcccat ttgctgagcg gcggaataag acattcgatt tgctggcaga
     4261 tgctttagaa gcttctttgg atgtgaaaag acttgatcga attattgctg agggaattta
     4321 gcgtgcaaac agcattgatc ggactggcgg tggcattttt cattgatctc ctgttcggtg
     4381 atccgcccaa tcgattccac ccggttgtcg ccatgggcaa tttcattcgg cacatccaaa
     4441 aaagatataa tacgggctct caaaaagaac ggtttctggc aggtttgggg actgttgttt
     4501 ttggcggtgc gctattctct ctgccctggc tggttatcct gccgttgtta acaaaattgc
     4561 cgatctgggc agccggcgcc ctgatgagct gtttcctcaa accggttttt tcttttcgca
     4621 atttgattag agccgggaaa gaaattcggc aatatttgct gataaatgat ctatccacat
     4681 ctcgtcgtat ggttggctgg catcttgtca gccgggatac cagccaactt tccaaaaacc
     4741 agctagcatc ggcagtcatt gaatctcttg cagaaaacat aacagacagt ttttgggctc
     4801 ctttgctttt cttttctatc ggagggctgc cggctgcctg gttttaccga tttgtcaaca
     4861 ccgccgattc catgatcgca tatcgcacgc cagatttgga gcacttcggt aaaagcgcag
     4921 cgaaacttga tgactttctg aactggctgc cagccagggc agccggaatc attctggttg
     4981 tatctgccgg gttattgcat ttgaatatga aacgggcatg gcacacgatg aggacgcaaa
     5041 atgatcgaac cagcagcccc aatgccgggt ggacgatggc agcggcggcg ggtgctttgg
     5101 agatcgtatt ggaaaagccc cagcattacc gattggaagg gggaacccaa ctgccggatg
     5161 aaaagagtat tgagcgtagt atttacttgg tcagtcttgc attggtattg agcctgttta
     5221 tttgtggagg aattatattt gttgtccatt caatctcatg aagaacacgg cggcgttgac
     5281 ccggcagagc tgaaaagaca tggactatgc ataggttccc tgattgattt ttccgtgaac
     5341 agcaaccctt tcggtccttc gccgtgtgtt gtcgacgcat taaagcatgt gggcatttcc
     5401 cgatatcctg acagaaattg cagcgagttg gcaggtcgcc tggctgaatt aaatcatgtc
     5461 agcgaaaagc gaatattaat cggaaatggg acgactgaac tgatctggtt gatttgccat
     5521 gcatttttac gaccaggcga tcgcgtgttg attatcggac caacgttttc ggaataccat
     5581 cgcgcatcaa tccggctcga cgcttcggta acagaaatgt ctgccatgcc cccctatttt
     5641 gttccaccac taaacaaagc catcgacttc atccgagaat ttcatcctcg cctcgtcttc
     5701 ttgtgcaacc cgaacaaccc cacgggcaaa tatgttcctg atggggagat acaagacctc
     5761 attgcggtct gtggttttga gtcaattctg gtgatcgata aggcttattc tgcatttctg
     5821 gacggttcct tctttgaatt gaccattccg caaaatgtcc ttattttgcg ttccatgacc
     5881 aaggattttg ctttggctgg tttgagagtg ggctacttgc tcggcgatgc ggatagcatt
     5941 gaaaagacga aagcttttca gcctacctgg agtgtgaatg cctatgccca ggctgcaggg
     6001 ctggcggtac tatcggaccc ggaatattat cgaaattcgc ttgcattact gaaaagaatt
     6061 caggtttcgt tcttcgacga aatccgctcc attggctacc gcctgataac atcggatgtg
     6121 catttcgcgc ttgtccattg cgggcgtcca gccaatcttt tccgttctca gttgctccgc
     6181 tcaggtctgc aagtgcggga ttgtacttct tttgggttgc cgcactacat tagaatctgt
     6241 actcgtcttc aagaggagaa caaaaaactg atcatggcaa tgaaagccat agaaacaatt
     6301 gatcctggca gaaatgatgt tcaggcaaac ttaccgagaa aggatgcaac agacgatgat
     6361 acttccagcg atcccactct ttaatgaaaa aagtgcagaa aatgcacgca tccgccagca
     6421 acgattggca aagcccattg gatcgctggg gatgctcgaa gcgctttcaa ttcaactggc
     6481 tggactcacc ggaaatgaac atccgcgttt ccatcaaaaa gaagtgatca tcatggctgc
     6541 tgatcatggg gtagcacaag aaggcgttag cgcttaccca tcagatgtga ctgccaaaat
     6601 ggtaaagaac tttcttacgg gtggtgcagc ggttaccgtt ttggcgcggc aagccaatgc
     6661 ctcggttacg gtggtggata taggagttaa tgacgatctt gaggacgttc ccggtttgct
     6721 ccatcgaaaa atcatgaggg gaacctttaa tatgctgcgt gagcctgcaa tgacccgcgc
     6781 tcaggctgaa gaagccattc aggtgggaat cgatgtcgtc gatcaacgcg tcaaaggggg
     6841 gatggatctt gtagcgaccg gcgaaatggg aatcggcaat acaactcctt catcagcgat
     6901 cgctgctgtg tacacgaaac tcccagtgga aagggtgacc ggacggggca cgggattgga
     6961 tgatgctgga ctgaccttga aaattaaagt gatctgtgag gcaattgccc tccatcagcc
     7021 gaatatccaa gaccctctgg acgtattggc aaaaatcggt ggtctggaaa ttgccggtct
     7081 gacaggcgtc attctcggcg cagcgtctca cagaataccg gttgtggtgg atgggttcat
     7141 ttcgggcgct gctgctctga tcgccgtaga gatgctgccg gatgtgaaac cgtttctgat
     7201 tgcttcccac caatcagaag aagttgggca caccgccatt cttcagaagc tagggcttaa
     7261 accgctgctc aacctggaat tgcgcctggg cgaagggagc ggggcggtat tggctttcca
     7321 tcttgttgaa gcagcaactg gcattctgaa tgaaatggct acattataaa gaaaagggag
     7381 gtatctaaaa aacatgaaat actttgctgt agcaatctca ttcctgaccg tttttcccgt
     7441 gaaggtgaaa gagccaatcc tccccggaga ccttgggaaa tcttcaagct ggtttcctct
     7501 cattggggtc ttcctgggtg gactgactgc cgggatattt ttcgtgtgct cgatttttct
     7561 tccccctttg gtgacggctg tgttgacgat cgcaatttgg attggtttga ccggaggtct
     7621 tcacctggat ggtctggcgg attgttgcga tggaatgtgg aatgcctcta acccggaacg
     7681 cagattggag atcatgaaag acccacgcct cgggtccttc ggaggaattg gactgatcct
     7741 atgcattctg atcaaggttg cctgtctcaa cagccttcca tctggagtat tttggacggc
     7801 aattccgtta gccgtgtcca cggcgagatg gcttttgtta tgtgccggaa aacaaaaaat
     7861 ggcgcgctca acaggcatgg gggctgattt ttcgagcggc atcacccccc gttccatcat
     7921 tcctgcactg ctgacgatcc ttatggcaac cattctttcc gggtggaaag gaatactgat
     7981 cgttgcgttc acccacggtg tagcctggct ggttgctcgt gtggcgataa aacaattggg
     8041 gggcttaaca ggagatgtgt tcggattgat cgttgaactg tctgaatgca tcgttttgtt
     8101 aggcttttct gtcggttgat gggcagagat gaagaagata aagaaagcac ctttccgtat
     8161 cgggaccaca tcgtacatca ttcaagatga gatattgccg aatgtgcgtt ttctcgctga
     8221 cctggttgat gacattgaat tggttctgtt tgaagtggac gacggaccta acaacttgcc
     8281 ggatcagaaa accgttgatg aattaattca gattgctcaa caacatcacc tcagctacac
     8341 tgttcatctc cctctggatt tgcgtctggc tggggatgat ggggtgcaac atatttcact
     8401 ggtaaaagct cgcaaagtga tcacctgcac tcgagaattg catccccatg cctatgtact
     8461 acacttggat ggcaaagata ttcttcatcg aaatgacccc atatccctgg caagatggaa
     8521 ccgacaggca ttgctggcgc tgcaacaggt atcagcatgg gttgaaaaca gcgagatgct
     8581 tgcggtggaa aacctggaac attacccgcc agggctgtgg gatgaagtca taaaacgcgc
     8641 ttcggtgagc cggtgcgtcg acgttggtca cctttgggta gataaactgg atcccattaa
     8701 ctttcttgca gagcgtatcg gcgaaacaag ggtgatgcac attcatggaa ttcgcgaaag
     8761 ggaccatcag tcgctcatca atgtgcctgc agatgaattg gagcgggtgc ttgattttgt
     8821 tatccaatcg aatttccagg gtgtgatgac aatggaagtt ttcagcgaag atgactttcg
     8881 atcatcgatg gaaactgtcg aaaaagtgct tgaaaaacta gaactggagg attgatggga
     8941 caacaactca cattgatcat gggtggggca cgcagtggaa aatctgacta tgcacagttc
     9001 ttagtgcaag aagatgaaca accagctttg tttgttgcga ctgctactgc tggagatgaa
     9061 gaaatggcga acagaattgc cgttcaccgc gcaaaccgtc ctgaaaattg gcaaaccctg
     9121 gaggcaccgc tcaatattgg aacggcaatt caaaacgccc ccccttttcc ctgggttctt
     9181 gtggattgca tcaccttgct ggtgagcaat ctcttaatga cttgtgctga gccggttgac
     9241 gaacgcggat atcatgcgat ggtcgaacgg gaaatggatg aactgattgc cagttattcg
     9301 attcatactg gcaagtgggt ggtcgtatca aatgaagttg ggcttggttt ggtgccggca
     9361 tatcccctgg gcagatatta tcgagatgcc ttaggacgag ccaatcaacg aatcgcaaaa
     9421 attgcagatc gggcgatcct catggtggca ggaattccca tgatcgttaa ggaataagag
     9481 gcttcattcc aaagaactta tcacaagcca atgaagcatt tccttaccgg gttgattcat
     9541 agaaagattg ccctgctaat cattgaacct gtcaaatgct ttatctgtgg agcaacaacc
     9601 gtttcaccat ccctttccac ccccctacgg aatcagtgaa tcttggcaga tggtttgcgg
     9661 tgagacaaat ttaagggagg gagtggcaga ggatggcatc gtcaagaatt tgcgtgcggc
     9721 aacgtgatgg tttcatcttg gatgaaccga cttttgcttt cgatgcggga accgaatttg
     9781 aaacccttcg gcagatgttg aacgataagg ttagcaaagt cgctttcttg gttttccatc
     9841 gctttttaac tgttggttga aatgacactt tttgcctctc tggtacaggt gcgttagaat
     9901 aaagattgtt tcagacatca agaagagggt aatacaatgc acacgaaagc tattctttac
     9961 gaaagccaag aacgggtatg cacctttgaa tgtgattttc aacgacgttg gaaacctgca
    10021 gcgatgtttc agcatctcac ggaagcagcc accagccatt cggagcaact gggagccggc
    10081 tttgatgtta tgctcagcca gaatttattc tgggtgcatt cgcgcatgaa gctcctgttt
    10141 gtcgctttcc ccaaagcagg ggatgaaatc atcattcgca cctggcctaa gaccattcaa
    10201 cagaagttgt tctacatcag agatttcgaa gtcctgacta gcagcggaca accactggcg
    10261 ctggcgacct ctgcctggct ggtgatcgat gctaccaggc ggcgtatgat tcctccacgt
    10321 tctctggata tcgacttgcc aagcttgccc gataaatctg gtttggatga acctttggaa
    10381 aaattaggtc tggatgacga cggcgaagaa cgtctacgcg tgcgggccgg ttattcggtg
    10441 gtagatatgt tggggcatgt caacaacagt cgctacattg aatggatctg cgacgcattc
    10501 cctatggaga tgtatgaacg aaataccctg gattggatcc agatcaacta tgaccgtgaa
    10561 gtccgaccag gcgaagaggt gggtttgctg gtgaaggaac aggcaggcga gaatgccgtt
    10621 tggcaggtgg tgggaaacaa tcacagcaac ggtacgctgg catttgaagc gttattgcat
    10681 tggaaggggc aatagtgtcc tcttttccgt aaaatcgaac agcaaaagcc gaattcaata
    10741 gtatttcaag attttgcaat acatttttaa cggctataga aaatattggg gtaggaaacg
    10801 tatcggaaat taacgctctt acaagtataa aggatgttgc gcataagaga ggggtgggat
    10861 ttttgatgaa acagttatct aatgcatcaa gtcagcggct cgtaaaggag agcagtgaga
    10921 gtagctacat tcgcaaaatt ctgatcgcta tctattttgg atctcttttc gttcatttcc
    10981 tctgtgtatt tttctttatc aatcagccaa tagcgttgga tgatatgtac cagtatgata
    11041 tgctaggaag atcaatagaa aatggaaatg gttatcgctg gtattcgaag acggatgttg
    11101 atgtattaaa accatattat tcccagtttt tggatctcga taatctagac ttccctgaaa
    11161 atggtatttt gacgacattt cgagcgccag gatacccatt ctttttggcg cttatttact
    11221 ctttggtccc cagttctttg cgctttgtgt ttgtgcgcat agctcaagca tgtctgatgg
    11281 caattctcac accgatttca gcctatatcg cttataaact aaaatgtaaa cccaaaacga
    11341 ttgttggagc agggattttt agctctttct atcccatcct gttgttttat ccgattggat
    11401 tggtttcaga gaatctctat attgttctgg gaacatcttc attgattgcg ttaatggtat
    11461 ccatagaaaa taaaaagaag gaaggatgga ttcttctggc tgcattgtta tgtggattga
    11521 cgatgctgac cagatcgatt tatgccatgt atgttttgtt ggcaggcttt tggatagtcg
    11581 ggtatagaca ggtcaagtgg aaaacaggtt tgctgtttat gcgcgtcgca ttcggaattt
    11641 gtatgccttg ggcaattaga aactctatcg ttttggggag cccctcattc gttgaaaata
    11701 gtcttggata taaccttttt attggttatc atccagaagg aaacggtggc ttcgtatcag
    11761 aaatagcaat tcagccttta aacattttag atgacggaga aagaaacatt ttttgtttcg
    11821 aaaaagcact caattttatt cggcaagatc ccgtggaaac tgttcgtagg tttttcatca
    11881 gggtgggaat gtttgtcgga cctgaaatca gggagtttac atttttctac acgaatgatt
    11941 ttttaggagc aattccaact tttttcctgg tattgatcta tctcgtatta acgattccct
    12001 ggctgactct cgtaatcctg agtcctgttg ggttttggta tgcacgggaa aaaggatttg
    12061 tggttcttgc actaattttt attttagggt acagcatccc tcatttcttg attctggcgg
    12121 aaccaagatt tcatcttgga attgttccgg tattaattcc ttttgctggt tatgcattcg
    12181 aaaatagaaa ggaaataaaa aattatcatt tcaggtttca aaaagatcgg ctatttttat
    12241 tgatctattt gactattttg atctgttttg gaattactct gtttcataat ctaaatgata
    12301 taaaagatgt ttttaacgct ggtggaaaca ctttaagata ttcctattaa atttctatgg
    12361 attgaaggtt atcagtgaag aaataatagg gtgcttgcgg aaatccacag tacaggctcg
    12421 gtttccaaat cactataata agtacactta aaagctgttt tattggaaat atcgcagcag
    12481 gtatttttgt ttgaactatt cctaatagcc gtgaccagtg tagtataaaa ttgattcgtc
    12541 gtggtcatcg caaacaaatc tcaggatggt ggtaaaacat acatgaagga tagtaataaa
    12601 aaagcaacga ctcttttaag agatatgagg attgtcaatg aacagttttg acgatttcag
    12661 cgagcgggaa caagcggtca ttaagctgct ggttcaaggg aaaagcaata aacaaattgc
    12721 gttgcagctc aagatatcga accggacggt tgagttccac ttaagcaaca tttactccaa
    12781 attaggcgtg agttcgcgca ccgaggcagt gctgttgttc atgaaagacc tttctgggga
    12841 acaggcagag gaacctgttg aaatcacctg gcgggaatcc acagatgcgc cgatggacga
    12901 accttcttac aatggggaaa aaagagaaca gaggaatcct atgaaaaagc gcacctggta
    12961 tctcatcggc tcaggactgc tgatcgcagt attcctggca ttggtgttct ttatagaccc
    13021 agagcataat ttgaaaaatt ttttcacccg gattattgag ggagattccg gcatcatttc
    13081 ggtggaagtg gctgcgccgc aggaagacgg gtcaatcatt cataccgttg aagaggggca
    13141 aacgttgtgg agcattgccg tggcttacga tgtgcccgtt aaccagttgc gctttctgaa
    13201 ccagatatcg gaaggctctg acgttattta tgtgggtcag cagctctata tccgaactgc
    13261 ttctactcaa atacctacct caaccccgat tgctgctgaa aatactttta cccaaaccct
    13321 ggactcgtcc agggtgaccc tcacggtgaa atggttctat atcgatcaaa ccaggcttta
    13381 tctggatctg gtggtttcgg gttatccact gccagaagac ttttcgccgt tgcagctcat
    13441 tgatctgcaa aatgttgtca tccaccgatc tgatggcagc ctgatcgacc ttgaacattc
    13501 gatccgatac ggaggaattg gtggtggggg aggcgagaat tctcaagatc aggagcccca
    13561 gtttttcgaa ggcattatcg atgtgggtct accctcttcc ccacaggaga attcacagga
    13621 agaaccctac gtcatggata ttttgattgg tggagagatt tacgccgaaa atggcgaagt
    13681 ccgtgttgtt ctgccggttg tgccatttca tattgaagta aagccgacct atgttgggca
    13741 gctaactttc gccgcagatg aatcagccgt cattgatgag aaaaccgtca cattcaaagg
    13801 gatggaaatc aacccgtcca atgcggcagt tttgttttgc gttttcgatc cgcaaggaag
    13861 tcaatggttg ccatccattc atcttctcta taaaggaaat gtgttggacg aagccggtct
    13921 tgccctaacg gatggcgatc ccagcagcca gatgtgttat cgtatttatt acacccgctc
    13981 cttccagttt gacccaaaaa acgatccccg aaccaacatc gccattttgg tggcgaaatt
    14041 ggtcaaagac caaccggaac gtctccctta tgaactggtc gctgtcgttc agaacgatct
    14101 ggcagcgcag ggtattgaat tcaattatgt gatcatcagt cacggatcag ggattgaggt
    14161 gaccaagaga ccagcgggca tgacggaaga agaagcgcag ttgatcattc acaatgcttt
    14221 ggttgcggaa gctattcccg aaggaacgct gatttttcat ttgccctaaa gcatgcgaaa
    14281 aagagttcca aatctcgatt agaatttact gcatggaact tcccattcaa aatattcgaa
    14341 ttctggtcat tgaagatgat cgggcggtgg cgcgcagttt gcatgatgca ttggtgaaag
    14401 agggttatag cgtacagcta tgtgattcgg ggggcaaggg tttcgaagct gctcaacaaa
    14461 accaacccca tttgatcatt ctggatattc gcctgccgga tggtttggga tttgactttt
    14521 gcaaacagat gcgcaaggca ggattgtatc aacccattct ggtgctcacc gtgcgcgacg
    14581 aaccgatcga caaggtgctg gcgctggaga taggagcgga tgattatatg accaagccct
    14641 acaaccccca ggaactgcat gcgcgcatcc gcgccctgct gcggcgcgca tacggagagt
    14701 tctcatccac cgaatccaag ctggtctttg tgggtgacct gatcatcgat gtgttgaaag
    14761 ccacggtgtc tcgcaaccag acggtcatca acctgacccc aacggaatat cgcctgtttg
    14821 ttcatatggc aaaacatccg gggcaggtct tcagccgctc tcaattgagc aacagcatcc
    14881 gtaattgtga caatgaagaa gtggatgaca gccaaacgat cacggtttat attcgcagac
    14941 tgcgcgaaaa gttggaatat gaccctgcac atcccaagct gatccttacg gtccccggca
    15001 ttggctaccg gctctcccta tctcctgaaa cccattaata aactgtaagc aaccgtatag
    15061 agcgctgaaa tattcccgtt gtagacttag cttcgatttg catattcgaa ttcagaaggg
    15121 agagactttc tccttaacat tgacgcaaag caacagaaaa tgctctactg catctcacgt
    15181 tgtctgaacg tgccagagaa ctctcccttc gaattcggaa agaaatccag cacacaaaaa
    15241 atcaggagaa aaatgaagaa atttgttttg agtatcttca tggtattggc attgctattg
    15301 gcagcatgcg gcggtagcgc tacagagaat tcagacgttc aggatctggc tgcacctgcg
    15361 gttgcgacaa catccgaaac ccccgctgtt tcggataacg gaccggcaca accatccggc
    15421 gatcggggtg cggtggcaga tggcgaggtt tcagaactga cacggttgtt gatcggcata
    15481 ttccaattag aaagcaccga tctggctctc acgacggacc aggctgctgc gctgattccg
    15541 atgttgaatt cctacctgga actcgagcag agccaaaacc ttgcttctcg acagcaaggg
    15601 caacaagctg caaccccagg tacatctacc ggtgccatgg aaagtcaaca agccaccatg
    15661 caggcgcagc aaattgtttt acaggaacaa cagagcgcgc ttattgtaca gatcgaaaca
    15721 cttctgtcgt ctgaccaatt gaccttcatc acttctcaag aattggatca agaagcggta
    15781 agggcattcc ttgaagaaca agggctttcg gcgggcggtg gaaatcctgg ctctggtggt
    15841 cagggttcag agcagagcat atctcaaggc actcctgccg ttgatcagcc agggaacgtt
    15901 ccacaggtac aaggtactcc aggaaacgga aatggcggac cagggggaaa tctgcaagat
    15961 ggttcggcgc ctgcatcaat gactcccgga gcagaccagg gcttcggaca accaaatcaa
    16021 ggcggaggtg gattgggatc cacttcacga attctcattg acgcattgtt gcaattgctt
    16081 gagacgaaga tgagtgatta gactgacctc agatcgaaaa agtgctgccg ggtatccggc
    16141 agcactttgc tttgaaaagg cgtgcatata aaaaaattgt aatatgcggt aaagggggat
    16201 gaaatattgg ttcgctacac ttactcccag cctgcctgca actttctatg caatggagat
    16261 caacgtgaaa ttcaaaatga ccattccgga tgaaataaaa aaagaaaggc gcatagccat
    16321 ttctatcaat ttaagaagag ttggacagat ggtcacacac ccacgccagg tctttgccga
    16381 gttggaaaac gaaaacgaca aagcagattg gaaaagcccg atcattgtta tcggattgat
    16441 cctcattctg gcgagtttga tgtcggtaac cagttcatcc ggcacttccg caactcaggc
    16501 acaaacctat ggttctaata cctctaccgg tttttccaat atgagtcctg gaggtatgca
    16561 gggtggcggt ccgatgggga tgggaggtca gacgcaatcg acagcaacga ccgatcaatc
    16621 aagcgctacc gatgaaactg caacctcgac caccgcaagt tcttccaccc tgctctccaa
    16681 gatcctctcg gctgcaggag cactgctcag ctttgtatta acctggctgc tggtcggaag
    16741 cctagcaaac cttatttcca tttcattggg aggtcaaggg aacaccaaac tcgcaacagt
    16801 cttttctgcc tgggcgtgcg ttcccatcgg aattcgttct ttgatgcaga tcatgtatgt
    16861 gctcgccacc ggcaattcga tccaggcggt agggctttct ggttttgccc ctacctcctc
    16921 gaccaccctg gcgactttgc tggaaaagat tttggcgcaa attgatatct acagcatctg
    16981 gcaaacagtg ctgctggtca ttgggatggg tgtcatgacc aaattggata aaaagaaatc
    17041 cattgtgatt gcggtgacca gcattgtcat tgttgttttg ctaaagtcgt tgctgggttt
    17101 gggcgtcgaa aaaatagcga gcatcaatgt gagcaatggc ttgttgaata cgatgatgcg
    17161 atgacgaaca tcattcaagt gcaagcactg agcaaggtct acgaaatgga cgacgatgtc
    17221 caggtaatcg ctctggcagg ggtgaatctg gagatcgagg aaggttcatt tgttgccgcc
    17281 atgggtccgt ctggatcggg aaaaagcact ttgatgcaca ttgtgggtgg attggatcgc
    17341 ccgacgtctg gttcggtcga agtgggcact tatcatgtca ccacgctgga tgagcaacag
    17401 ctagcattct ttcgcagaaa gatgatcggt ttcatttttc aatctttcaa cttgatcgca
    17461 accatgactg cgctggaaaa tgtggcactt cctttacggc tatgcggtat ccccagaagc
    17521 gaacggttag agcgcgctta tcatctttta gagaaagtgg gattaaagga tagagtgtat
    17581 cacaaaccga cacagttatc aggcggtcag cagcagcgca tcgcggtggc gcgcgcgctg
    17641 gctaatgacc cgccgcttat cctggcagat gaacctacag ggaacctgga cactgccagc
    17701 ggtgaaaata tcatggtcat gctgcaggaa ttgaacaaga acgggcgaac ggtcatggtc
    17761 gtaacccacg atccgcgtat aaccaagtat gtcaaccggg tgattaatat gctggacggt
    17821 catttgcaat gaaaaatata caagggatca ccattatgaa aaaacacttg atctttatct
    17881 tactgattgg catgttgatt ttttcgggat tcactttttc ggcatctttc aaagatacgt
    17941 cgaatggaaa agctgtttta caggaagaaa caaccgaagc tacggaaacg gtcgcagcaa
    18001 ccgcaacgac tgaagttgca gcaactgcaa ctgtagcgcc cacttcgacc acggtcccaa
    18061 cggcgaccac tgcagcggct tattcccgcc ctctgctgaa tttaaccagt tattcctatg
    18121 attcttcgga agtttatgcc ggaaatgtct tcagcctgga ttgtgtgttt caaaacctgg
    18181 ggcagcagcg tgcgtataac gtggtcatta cctactccgc caattcgctt acggcagaaa
    18241 ataatggtgg cgtggatgtg atctcatcgc ttggcgtcgc tgaaagcgcc acctcggagc
    18301 aggattttta cgtgagcagc agcatcagtt caagcccgat cacagtgaat atcagcattg
    18361 aatatcgaga tcaagatgga aaatcgtaca cccaatcctt tgcggtgtct gtgagcgtta
    18421 ctggatatgg cacgtactat acttctacgc ccgaaggcag accccagatg gttgtggcaa
    18481 gttatgaaac cgacgtcgac ccactagaac cgggtaccag ttttgtgttg accatgaatt
    18541 tacagaatag agggtatctc actgcgcaga acgtcagtat ggtatttgga gggacggctg
    18601 actccagcag cacgaccagc tctgctacaa gttcaagcac aaatttcttc cctctcggct
    18661 cttccaatgt gcaggtcatc ggtaatgtgg gaattcaggg ttatgttcag gttcaacaaa
    18721 ctctggttgt caattcggaa accacgccgg gagtgtaccc gctgaccttg agttttacct
    18781 acactgatga agacggaaat gaatttactg acagccagat catcactttg ctcgttcatt
    18841 tggttccttc gatagaaatc agtttttacg agacgcctga cgtgttctat gtgggagaag
    18901 aggatactct tcctattcag gtggtcaaca taggtagcga ttccatcctg cttggtgata
    18961 tttacatcaa tgtggaaaat gccactttat caaacaacct tacctttgtg ggcacgctgg
    19021 aaagcggagg cagttttacc attgatacgg atatcatacc ccaacaagca ggaagcctgc
    19081 cggttacagt aaccatcaac tatctggata atttcaaaaa agcacagacg atcacccaaa
    19141 cgctctcgat cactgtttca gatacacaaa tgccggcagc caccatcgat ccggctttgg
    19201 tagccaccga aatggcaaga atgcccgctg gaggaaacca aaccagcagc gaccttggca
    19261 gcattcttct gcgatttttg cgcggtatga tcgggtttga cagcagtgct tcgaccgggc
    19321 aaaaccgcag caatccgggc ttgatgaaca ccaattgatt cagggatttt tgacgcatca
    19381 tgagttttat cgaacttgcc aaatttattt ttgagaatat caaacgtcaa aaagggcgtg
    19441 tcatattaac ctcgctgggt gtgatgatcg gttcggcttc catcgtgttg ctggttgccc
    19501 tggtgggtgg tttgcagcag tcagctacag cacagtttac cagcaatgcc agcctgtcgg
    19561 agatcacggt gagcgctgga tttagcggtg gtttcagcat gatgggtggg ggcggcagtc
    19621 ctggtggggg aggaggtatg gggggtggcg gttccaccgt gcgcaccagc agcgaatcgg
    19681 aagaattgct gctgacggat gacgttgttg aaacgatctc tttccttgag catgtggcga
    19741 gcgttagcgc cgagcagagc gtgtcaggca attcaaccct gatatatcaa aagtatgagg
    19801 gaagggtcag tttgacaggc gtagatgtgg atgacctgtc tgaattgggg ttgagcgtgg
    19861 atgagggaga aacggagttg aattcgggca ccatcattct gggatacagc gtgagcgaga
    19921 acatgtaccg ccccaattat tctggcagtg atgcaaccct gaccacgaac ctgctgggca
    19981 agcaggtgaa agtggaaatt acaaaaatgt ccagcagcgg cagaagtgtg aaaaccatgc
    20041 gttttcgggt ggtggggatt ctggcagaat ccggagatca gtctgattat gctgcctaca
    20101 tcaccatgac ggatgccgat acgatcgtct cctgggtcaa tgggcaaagg gtgaaccaca
    20161 gcactgatgg gtattcatcg ctcaccgtgc gggtggatga ctcagacaac gttttggctg
    20221 tagccgatga gatcaccgct atggggtacc agacccagac cgctcaatca ttcctggaaa
    20281 acgtcaactc cctgttttca tccctgcaat tggtgttcgg tggtgtgggc ggcatcaccc
    20341 tgttggtagc ggcgctggga attgccaata ccatgactat ggcgatcttg gaaaggacga
    20401 aggagatcgg cttgttgaag gcattgggcg ccaccaacaa ggatattctc ggcttgttca
    20461 tcggtgaagc agcagggatc gggttgatcg gtggaatggg tggcttggtg atcagcatgc
    20521 tgttggggtc tgtgatcaat gctgttggag ggtcttatct gacggagcaa gccactgcga
    20581 gcggaacgac cgtaaccacc agcatcctgt acatgccctt ctatttgcct ttgttcgctc
    20641 tggcgttttc caccatcatg ggcagtttat cgggattgtt cccagccatg aaagcccaat
    20701 cgttgcctcc ggttattgcg ctaaaatacg aataagatga aaaaggaaaa aagacgttta
    20761 ttcaattcat tacgcgctca gttgccccta tcgtacgctg gaattgccct gattactaca
    20821 acactgatcg gggcagtgct gctattcatt atctggaatt attatcacgg actggagaaa
    20881 caataccttg aatccaatct gcatggaaca gccaataacc tgacgcgtat tgtggaagaa
    20941 aatgacataa cggctacaac atcattgatt gattacagtg acgtttttca gaatcaagca
    21001 aacataaccg cttttttgat tcagtcccgc atgcgtgtgt tggacaccaa cggaaatatt
    21061 atcgctgatt caggatcacc caccagcagt tggaacatca ccatttccaa accaaaaaac
    21121 tctgataacc agcagagggt tcagaatgag ggagaaaatg atgagcgtcc ttctttttca
    21181 ttggaaaaat ctgccggaca gccctcgcaa gattccgaag gaaaccctcc cccttactct
    21241 atccaggcaa atcctcatat gttcggatat ctgctggaag attccatgaa tgaagaagga
    21301 aagcgctctt ctgtggttgc caaagaagcg ttctacgata aagatgaaaa catccttgga
    21361 tacgtcgaat tgtcggaagg tccggggtat ggtcaacgca tcattttcaa cgttttgcgc
    21421 ggttgggcta ttgccagcct gatcggtctt atcgtatcgg tcacctttgg atggatcatg
    21481 agccgcaaac tgaccaaacc cttggtggag cttgaaaaag ttgcagtgga aatgaaaaag
    21541 ggaaattatg agatccgctc accgatcttg aaacccaatg agctggcttc cctttccgga
    21601 acgttcaatc agatggctgc tcatattcag caaagcattg aaaccctgcg ccagttcatc
    21661 tcggatgccg cccatgagat cagaacccct ttgacatcgc tgcgcgcaga cctgaccctg
    21721 gcattatcgg aaaattccag aaagggatta aagccgatcg tcaaacgatc gcttgaacag
    21781 gtagatcgcc tggatcaact ttctcgtgat ttgctcgatc tttccaaatt ggaatcgcgc
    21841 aatacggtcg ccccggtgga agaggtgaat ctggcagatc tgctgacaga actgagcgaa
    21901 gtgcacgcat ccgccgcaga acaggcgggc atcacctttc aatttcaatt gcctgcagat
    21961 gcgttgatcg tacgaggcga taaaggtcaa ctgtatcgag ctgttggaaa tctattggac
    22021 aatgcggtga aattcacccc cgtgggcgga agggtagttc tctctttgac cgcaaggaat
    22081 aactttgctc agataaccgt tgaagacagc ggcattggca tcccggcgga tgaacgggaa
    22141 ctgctcttca agcgcttcca tcggggtaag aacacccagc gttttcccgg ttccggattg
    22201 ggattagcga ttgccaaagc tattgtggaa aaacatcaag gcgaaattgg cttgatgcct
    22261 gacgctgatc gcacgatctt cttcatacga gtgcctttgc tcgttcataa aaaatagatc
    22321 tcactgaatg atcattgatt cgagggaacc atgattgtcc ccgttgcggt tcataaattc
    22381 ggtacaatag ggttcaacaa actatggaat tccaaatatt caccccacat cacgtagatg
    22441 atgcggcaac cttgttggtt gcgcaatcca caagcatgtt gaagcgcttt ccgcttttgc
    22501 ccgagcgcgt tggcaatctg catgccgcca aaatatttct gtccgctttg ctggaaaaat
    22561 cggaagcgcg cggcatgctg atgcttgaaa agaataaagt ggtcggatac ctgctgggat
    22621 attatgacga caatcctttc tttggaaggc atgtgtgggt tccttttgga ggaaccgttg
    22681 tcccccaatc gcaagcgtat ggggctatac gggcgctcta tgcggcagcc ggtgatatct
    22741 ggatcggcga tggaattctg aaccattatt gggtttgtcc ggcgttggat gattggctga
    22801 aagcgggttt ctcgctcagt ttcgggcagg aacaggttta tgccgttgcg cctttaaaag
    22861 agcgagaagc tggaaccgct ttacctgctg gcataaccat gcgggtggtt caaccaggtg
    22921 atgcgcagca attagcacaa aaggggcact ggatcgccgc gcacctcaac ggcgctccgg
    22981 tgtgggagcc ggtgccccca gagcatcttg agaacatccg cccggcttat gcagagctgg
    23041 caaatgatga tacttccacc acctgggtgg cattggatgg ggaggagatc gtttcgtatg
    23101 tcaccatcta cacggtagat atcggcgcga tccactacct gggatctcaa aaagcggcgc
    23161 actttgccac tgccgcaacc gacccggctt atcgtgccag agggatcggg cgggctttgg
    23221 ctactcagat attcaatatc gctggcgaac aggggtatga gacgatgttt actgattggc
    23281 gcatcaccaa tctggaagct gcgcgctact ggccgacctt tggttttgag ccgtttgcgt
    23341 atcgcctgtt gcggcgggtg aatccgcggt accaacgttt cgagatgccg aagtaaacct
    23401 tttattcatt gtctaacgaa gcttcatctt ccagttccga aaaggcatcg atcagatcca
    23461 aatacacgcc atcaaaaccg ctgtggatga tctttcccac atatgaatcg gcgctgccgg
    23521 tgatgagcgc tttccattct ttattccagt attgcacgcg gtagttgcct gcccagttcg
    23581 ggttttcttc caacagccat tctggagggt tgctatccca ttccgctttc caatacgggc
    23641 gatacgcttc ggcttccccg atgctcatgt aacacaacac caggcggcta ccgccgttgg
    23701 ctttgttgcg caattgttca acctcatcgc gggtgaaggc ttcatccccg aggtacaggt
    23761 ccatgataag cagatcgtaa ttggtggcag tgaccgcacg gatgaaatcc tccttggagg
    23821 aatagcgtgc cgggttgatc aaatataaaa aattctgcgc ctgacctaac gtttcgatag
    23881 tatcgtcatt ttcggaaaac ggacctggtg gataggaagg gatacgatcc aactcacgcc
    23941 ggtcggcagc caacgataga caactcaact catgatttcg ctggtaggaa gcatccattt
    24001 tttcaggggt ggagcagtaa tcgatcacca gcacagccag accgttctgc ctggcaagat
    24061 cgaggtatgc gatccacagg gatgtgacgg ttggatcggt ggcaaggtca tccgcttcaa
    24121 agccgtaaaa gagatcttcc tgcccaatgc catcgatgct ggcgatatac gcacggtcag
    24181 cttccgagtt gacgtcatcg ccctgtacca gcagttcctg cccgttttga gggatgacga
    24241 ggaaatcggg gtcaagggaa tgagcatagg aactgatctc tcccacaaac tgacgcattt
    24301 cttgacggta attgattgga atttctgcga aagacggaac gcatgccgat aaacacacaa
    24361 aaattgaaaa gaaaaaaagc cgtaaaaatt tgcgcataat taaggatatc gcaaatgccg
    24421 tatcatggaa aaacgttctt cgtcggtcaa aatgatcact atgaattggt tcagaaagaa
    24481 tggcttgtta ttgatccatc agcgccaata cctggtcaat tttcggcaaa gaaggtt
//
LOCUS       DAOSDS010000026        24360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_925_length_24360_cov_4.454680, whole genome shotgun sequence.
ACCESSION   DAOSDS010000026 DAOSDS010000000
VERSION     DAOSDS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24360
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_925_length_24360_cov_4.454680"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLL88_04760"
     CDS             <1..301
                     /locus_tag="PLL88_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HOJ00908.1"
                     /translation="PSSEGIIAEVRDNPNAIGYDGLGYVTNEVKMIALDNGVGYILPS
                     EETVSNHTYPIARQLYMYTNGEPQGIVKNYIEWIRSSEAQQYVLDLGFIPYNQGE"
     gene            310..1188
                     /gene="pstC"
                     /locus_tag="PLL88_04765"
     CDS             310..1188
                     /gene="pstC"
                     /locus_tag="PLL88_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOJ00909.1"
                     /translation="MKDRLVTRIIETAGFSSIIIVLLIFLFLLREGMPALNEVAPGDL
                     FTTRWYPIEGYFGILPLFSGSLLVTLFSILMAIPFGVGTAVYIAEVAPRRVKEILKPT
                     IEILAGIPSVVLGFIGIVFLSPALREWLNLPTGLTAFTGAVLLALISIPTIVSVSEDA
                     LNAVPQSYREASYALGITRWQTIWRVTLPAARSGVITAVMLGISRTLGETMAVMMVTG
                     NAPTIFKGIQWLFKPTRTMTATIASEMGEVASGSTHYHVLFFIGIVLFLFSLIINIIS
                     NHFSEKNRKDKEQVVS"
     gene            1185..2060
                     /gene="pstA"
                     /locus_tag="PLL88_04770"
     CDS             1185..2060
                     /gene="pstA"
                     /locus_tag="PLL88_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOJ00910.1"
                     /translation="MNIPGSRDSLILRRVKDSLGYILIHIISFFTILPILGIALYIIV
                     NGAPTLTWEFLTSMPTNGMKAGGILPAIVGTLYLTIGTAIFSVPLGVAAGIYLAEYAP
                     RNHWTRLIRIAIISLAGIPSVVYGLFGLGMFVLFLKFGNSILSASLTLAIMTLPTIIS
                     TTEEAMRVVPERFRVVCGSLGASKWQGIQRIILPEAMPGILTGVILGVERAAGETAPI
                     LFTGATFFLPHLPTSPLDATMALPYHIFVISTQVPGMPLQIQFGSVLVLLIFVLLMNV
                     WATVIRNRARRRRQW"
     gene            2062..2823
                     /locus_tag="PLL88_04775"
     CDS             2062..2823
                     /locus_tag="PLL88_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HOJ00911.1"
                     /translation="MSDPKISIRKYCLRYSDGVEALKNIDLQIHPHAINVLFGPAGGG
                     KSSLLRSINRLIDMADVEETSGEILFNGENILSQDTEVPWLRRKIGMVFSRPIPLPKS
                     VFENVAFGLEVAGEKDEKLIRQTVEQALKQAYLWEEVYDRLDDPGTSLSGGQQQRLCL
                     ARILALQPEVIMLDEPTSALDPVTTARIESFLQEIKERITIIIAPHNMSQSARVADYA
                     SFFLQGELVEFGEGSKLFVNPQDKRTQDYVEGRFG"
     gene            2944..3402
                     /locus_tag="PLL88_04780"
     CDS             2944..3402
                     /locus_tag="PLL88_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOJ00912.1"
                     /translation="MFEKAKKTMPLANAILTGDSKQVRRHLEAGVDPNQPIPGEDGCP
                     LHFAANSRADIIRLLIDHGADVNVRDGNGKTPLHCAAVTANLENMRALLENGADVNAA
                     DNDDNTPLMLARRGTPAAAFFANLGVPSNSEDDLKQKKAADLLLEYGAKE"
     gene            complement(4140..5639)
                     /gene="xylB"
                     /locus_tag="PLL88_04785"
     CDS             complement(4140..5639)
                     /gene="xylB"
                     /locus_tag="PLL88_04785"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOJ00913.1"
                     /translation="MDIYLGIDISTTSTKTIAIDSLGKILAVSSSEYSYETPFPLWCE
                     QNPDLWWEATKKSIRGCIAQAGLKSFQVKGIGLTGQMHGLVILDEKGTVLRPAILWND
                     QRTQQQCDEIRDLIGKEEFIKTTGNDVLTGFTAPKIVWVKEQEPQLFEKIRHLLLPKD
                     FVRYKLTGEFALDRADGSGTVLFDLKERNWSYDLIGRLGLDPAWFPPTFEGTQITGWL
                     TKQAGAEVGLMQGIPVFGGGGDQAASAVGTGAVKEGILSISLGTSGVVFASTDSPRIE
                     PDGRLHAFCHVVPGKWHLMGVMLSAAGSLRWFKDTFRKETNFDDLVAGAEDIEPGSGG
                     LLFLPYLTGERTPYPDPLARGGFIGITVRHTIRHFTRAVLEGVAFGLRDGFELMNEAG
                     LPRIEQVRITGGGSKSLLWRQIIADVLNAEIVTINSEEGAAFGAALLAATGTGAFDSV
                     EQACANTITVRDRVVPGSAARIYETIYSEYRSLYPSLKGTFHRIAGNVS"
     gene            complement(5650..6576)
                     /locus_tag="PLL88_04790"
     CDS             complement(5650..6576)
                     /locus_tag="PLL88_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00914.1"
                     /translation="MDRKEKRFLDLDCLEINIGHIQLLISKSVGPRILSFCLNGGENI
                     FAEVPGQYLEHPGKGKFYFYGGHRLWCGPEDPSLTYVPDSQPIIVNETSDSIELLQEV
                     DSTLGIQKSMQIMPTEFENILIVDHHIRNAKDKPFRCAPWAITQLKLGGTAILPQQMG
                     GMDENPLLPNRSIALWPYSDVKDPRFNWDNQFIAIHALPIDKALKVGMANPYQWMAYY
                     RNNLLFVKYASNYPTRQCIDYGATSQCYCNSKFIELETLGPLSDVESGKEICHREIWR
                     AVEDPFVEFSLSDLKDFFLHDEMTKICREKLF"
     gene            complement(6586..7755)
                     /gene="xylA"
                     /locus_tag="PLL88_04795"
     CDS             complement(6586..7755)
                     /gene="xylA"
                     /locus_tag="PLL88_04795"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOJ00915.1"
                     /translation="MTTKYDPKPEHKFTFGLWTVGNRGGDPFGAEVRDPKSPLDLVYL
                     LAEVGAYGVNFHDNDLIPIDSTPEEIETIKKNFKKALKDTGLVVPMATTNLFYDPIFR
                     DGAFTSNDAYVRNYALQKTMKSMDLGVEMGAEVFVFWGGREGTETDASKDPVESMKRM
                     RGAVNFLCEYALDQRYPVKFALEAKPNEPRGDLYNATTGHMLGFISTLDHPEMVGVNP
                     EVAHEQMAGLNFSHGVAQAIDAGKLFHIDLNDQVFGRYDQDFRFAAVNLKSAFFLVKL
                     LEDHHYAGSRHFDAHAYRTEDEEGVKDFARGCMRTYLILKEKVDQFNHDAEIQGLLGE
                     LNKMDSELPQFKGPYSQAKAQALKIYPFDRRSRSVKGLRYELLDQLTNEVLLGVR"
     gene            complement(7825..9969)
                     /locus_tag="PLL88_04800"
     CDS             complement(7825..9969)
                     /locus_tag="PLL88_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ00916.1"
                     /translation="MEVKGKPEQQSYLDTSLSFTDRINELMKLLTLDEKIGLISYTGK
                     DIPRLGIPAYNYWSEALHGVARNGRATVFPQAIGMAATWDKDMVYEVATAISDEGRAK
                     YHQALRRNGYTDQYQGLNFWSPNVNIFRDPRWGRGQETWGEDPFLTGELASAFVKGMQ
                     GNDPKYLKTAACAKHYAVHSGPERERHTFNAMVTKRELYDTYLPAFKKLVQDAHVEAV
                     MGAYNRTLGEVCCASKLLIEDILRGEWKFQGHFVSDCGALSDFYLSHKVTKDAAESAA
                     LALKHGCDLGCDHIFDEIPEAIQRGLLSEDDVDCALERVLLTRFKLGMFDPPEDVPFA
                     SIPESVIGCEKHRELAYQIATESFVLLKNKDNILPIQPSVRKIFVTGPNATSTEVLLG
                     NYYGFNERMVTLLEGITGRIPEGMGMEYTTGAMLKHPREIKETWAPGMALSSDLAIVC
                     GGSSSFLEGEEGESLLSPQNGDRSSISLPVSQISYIKELAMSGTKIVLVLTGGSPIAL
                     GEIEDLVDAILFIWYPGMEGGNAVANVLFGDVSPSGKLPLTFPHSLNQIPSFDDYSMD
                     RRTYRYIKEEPLYPFGFGLSYSKFEYRDLHLEDQNINLGDSLHLSFELHNCGDRSALE
                     VVQFYLSDLEASTIVPLHRLIGFERIGLFPGEKKKVEFQVTPEMMSFYDDDGKLTLEP
                     GEFRLEIGGCSPGKRGQDLGAPKPLSTVFKLN"
     gene            complement(10120..10761)
                     /locus_tag="PLL88_04805"
     CDS             complement(10120..10761)
                     /locus_tag="PLL88_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ00917.1"
                     /translation="MLKNSLIVMTATTMGTVCVCSLVAFVFARLQFRGKNFFSNLFTV
                     GLMFPINVAILPVYFVLRQIEQIGIPMIDTLWGVIFIQIAFQLSGNILILKGFFTAVP
                     SELQDAAYIDGCSNFDFFWRIMLPLVKPSLTAVGSLVMIASWNDLLVPLVILNSNRLW
                     TLPLGTMQFQGQYGQDLALISAFVALSAIPTIIFYFFAEKQIISGLTAGAVKG"
     gene            complement(11004..11948)
                     /locus_tag="PLL88_04810"
     CDS             complement(11004..11948)
                     /locus_tag="PLL88_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ00918.1"
                     /translation="MQLSQFIHDKHSVQENAPKNQRFQNRLVVILFLLPALVLFLLFV
                     IYPIFRSVYYSMYDWKGFGPAVDYIGLGNYKKIFSDEVFIKSIKNVLLIITLSLGLQL
                     PMALVLAVLVGRDLPGRSFFRTVFFLPYVLSEVNTGIMWMLLYNADPERGFFNAIFVW
                     LGLQPVAWLADMSVVIYAVFITLTWKYFGFHMLLYLTGLQNIPREIEEAARIDGANAI
                     QNFFYITLPLLGSTIKTSVYMSVLGSIQQFVLIWVMTRGGPVNASETMATYMYRFGFV
                     RFQLGYGAAVAIIMFILCMIFSLLYQALTRKPDYLSGL"
     gene            complement(12139..13464)
                     /locus_tag="PLL88_04815"
     CDS             complement(12139..13464)
                     /locus_tag="PLL88_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ00919.1"
                     /translation="MNRKIFSMISVLFIALFLFTACAKTTAPAEGEGLSAEAPAESEQ
                     QVTITWWHISTKDPALSDWQKMADDYMAAHPNVNIEITVLENEAFKTKLTTVLQSGEV
                     PDIFQSWGGGTLKEQIDAGLLKDITADLDADGGAWRSTFAPGALAVFSSDGKNYGVPW
                     DMGMVGWWYNKDLFAQAGITATPTTWSEFLECVQKLKDAGITPISLGEGDTWTGMHIW
                     SYLATRIGGEAAFTAAADRTGSFTDPAFVQAGVELKRLIDMQPFQDGYLGATHDEMQA
                     TFGNGRAAMELSGQWAPSTQAANSADLAGVQNLGMFNFPAVEGGAGDLTDVIGGGNGF
                     AIGKDAEPEAIDFVKYLTSQQNQTIIAASGTGIPVVKGAESGLTDPNLLLVQQSFAQA
                     KYFQLYYDQILPSAMGGIINDAVQKLFAGMATPEEVAAEIEAGAEDLIR"
     gene            complement(13660..14685)
                     /locus_tag="PLL88_04820"
     CDS             complement(13660..14685)
                     /locus_tag="PLL88_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ00920.1"
                     /translation="MNVEQNKRSITIRDVAKKSGVSVSTVSRVLNGKVDVAKATKNHV
                     LKIIDDLGYSTNLAARSMRSSKKNLIGLIMPDIAYPYAIEVMKGVNQAIAESEFDLLV
                     YTSGDVRKRGHITSEQKYITLLAKSISDGIIVVAPVTSEYNIDKPIVSIDPVTSDPSY
                     PSVHATNYEGSMEAVEYLIGLGHQRIGFIGGRVELESANQRLSGYRNALEEAGIPVDE
                     QLIVSGDYTTKTGIEGGQKLLLLEDRPTAIFASNDQMAMGVYQIAEEMELHIPGDFSL
                     VGFDNIPESKYLGLTTVDQFISEMGYAATQMLIRIINEIPEENQTLKIKTQLVVRNSC
                     RPYVGKN"
     gene            15811..16197
                     /locus_tag="PLL88_04825"
     CDS             15811..16197
                     /locus_tag="PLL88_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00921.1"
                     /translation="MGKTSTKDIQDFLCQCREIIQSNKVVPSNFWFISKKENRDTLLA
                     LGFSLQDIKYEISNLSLDNYCEGPCDAHEHKDQVWIFGKIMNNREIYIKLCVSDFDNA
                     GNKIKTLYCLSFHFAQKPMNHPYKRN"
     gene            16209..17189
                     /locus_tag="PLL88_04830"
     CDS             16209..17189
                     /locus_tag="PLL88_04830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HOJ00922.1"
                     /translation="MEKYCPICGKPTEWKEKISRDEFDVRGEKIFVEFPLFSCSICNT
                     EFEDLNSEIDPYALAYEEYRNRKGMVHPNQIKEFRRKYDLTQKELSDLLGIGGITLSR
                     YENGALQDEAHDQLLKFIFEPVNLYKAIREKERLFSAEKRNRLLPKLEEEAGISSYRL
                     LNSEKHTDIYTGNTEFNFQKAVNLIKAFTSNQSIFKSKLFKLIFYADFSHFKEHGVSI
                     TGLQYAHLPYGPVPNNYNSLIGVIMNVDSSIYIEERIANDYPGEVILSSTPAANIFSK
                     DEIETIRKIQDKFAQFTCKGIEEYSHEEVGYKKTQNGELISYEYAKDLQI"
     gene            17199..17435
                     /locus_tag="PLL88_04835"
     CDS             17199..17435
                     /locus_tag="PLL88_04835"
                     /inference="COORDINATES: protein motif:HMM:NF021471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HOJ00923.1"
                     /translation="MSGKNQHVVPTGNGKWGVRGAGNQRLTHITNTQAEAIEIGKEIA
                     KNQDSELLIHGRNGQIRSKDSYGNDDCPPRDKEH"
     gene            complement(17643..18806)
                     /gene="nagA"
                     /locus_tag="PLL88_04840"
     CDS             complement(17643..18806)
                     /gene="nagA"
                     /locus_tag="PLL88_04840"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOJ00924.1"
                     /translation="MNQLIKNVKIILKDQILLDNRLLVQDGKISAITNENVDIADCMV
                     IDGRGLYLSPGFIDVHNHGNSGFDTMDATINALEVIASFHMKTGVTGFLATTMTASCD
                     ATVRAIENAVEYSTTADSPASQMLGMYLEGPYFSIKKKGAQSEKYITGIHLDELKNYV
                     RRGKGLVRIVALAPELERSKEAITLLIDHGITVSAGHSYATYEQMMNGIENGISEATH
                     MFNGMRSFDHREPGIVGACLLDDRVTCEMICDGIHLHPAAMQIIMKMKGKEKVVLISD
                     SIKANGMPDGVYDYMGTKVSIKNNEVRLLDGTLAGSTLSLDRAVRNLVEMTGIDLPTA
                     VRMASLNPARQIGLSETKGSIEIGKDADIILFDENINVKRTIIKGVTVYEESL"
     gene            complement(18803..19564)
                     /locus_tag="PLL88_04845"
     CDS             complement(18803..19564)
                     /locus_tag="PLL88_04845"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ00925.1"
                     /translation="MQEIFFDKIEEILHKIRETQSESISTIAKKLSDILINGGIVYTF
                     GAGHSHMLSEEIAGRAGGLMQVRAILEPELTEIQGRGKSTQIERMPGYAKIVYEANAI
                     RKKDALIVISNSGRNPVPVEMAEEAKKADVLVIALTNLEHSKSVTSRCPSGKKLYEVC
                     DYVLDNCGVTGDAAIEVRGKPYSIGSTSTVAGAIILQALVVEIIGILLQKGNEPAILM
                     SGNLDGTDEYNQKIYMQLISKYPELLYMLDVWRRM"
     gene            complement(19582..20367)
                     /locus_tag="PLL88_04850"
     CDS             complement(19582..20367)
                     /locus_tag="PLL88_04850"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF016054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative N-acetylmannosamine-6-phosphate
                     2-epimerase"
                     /protein_id="HOJ00926.1"
                     /translation="MNTILSNLQNKLIVSCQAFPSSPLYGADYMQKMAMCAIQGGAGG
                     IRACWADNVKAVREITNLPVVGINKLMDDEPLSINKVYITPTFESAAEVIEAGADILG
                     MDCTPRGRTYDDIHKIVEQIRKHYPAVLIMADLSTLEEGVKAAEMGVDIVSTTLSGYT
                     PESLKVDPEKYRLLMQSSSSLDKASDLGPDFGLIQALRENVRVKINAEGRISEANQVV
                     KAMQCGADMITIGAAITMPEKITGKFVNAMKPACLSNVDFMDE"
     gene            complement(20453..22063)
                     /locus_tag="PLL88_04855"
     CDS             complement(20453..22063)
                     /locus_tag="PLL88_04855"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOJ00927.1"
                     /translation="MTIWQALLISIFGYFSSIYSPWLIGLLGGWYTIGRPLVSGLIIG
                     LILGDVQQGIIIGAAIQAMYIGLVTPGGSMPADVNFAAWIGIPLAIISGADASYAVSL
                     SIALSFMGVMAVYATASFNSVFVHKMDRYITDGKLEKAMNIPVVGQITNFVCRFIPIF
                     LANYLGAQFVPTMVSLIPEWLGGILTLLGSILPLVGFALLLQYVVKKKLDLIYYLIGF
                     ILVAVFKIQIIPVVLFGLLFAYMDLRYTQTDFSVLKKTKTEKTDDKKKLLSKKDVHSA
                     YWNWMFWNLSVQNFERMEAPAIIRMLGKVKEKLYPGNKEAQKELLARHEAFFNTEPYI
                     GSIVPGIVLGMEEQSAVNKEDSADLISGIKTALMGPFAGIGDSLYVGTLIPILLSIAL
                     GLSTDSGNVMGPVFYVISHLAIMLPLTWFLFKSGYSMGMESAQLVLSGGIKDKITQAM
                     NIIGLVVVGAITSMYVNVKTGLTFTQGDMVIDLNQTLNGLFPNLITVLLGLATYYLMA
                     EKKMKIGWLFLVFIAVAIVGYFTKVLAV"
     gene            complement(22108..22593)
                     /locus_tag="PLL88_04860"
     CDS             complement(22108..22593)
                     /locus_tag="PLL88_04860"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ00928.1"
                     /translation="MITLNRVDDRLIHGQCIVRVIVDFKIEKIVIVDDFTASNPILKN
                     IFNLATPPQAKSVIYSIEETRASLPPFIENSENVLLLMRSPTVALKLFEIHPNLKKEL
                     NIGPISYRPETIKVTPYTYLSREEIDAVNKIEERRVRVYFNQTIDQKIIEWNEVKDLC
                     K"
     gene            complement(22601..23029)
                     /locus_tag="PLL88_04865"
     CDS             complement(22601..23029)
                     /locus_tag="PLL88_04865"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ00929.1"
                     /translation="MSSANQNETVGIVVCTHSALADSFKQAIEMLMGPQECFETIGLY
                     EGDDIMDLSKKIIEQIRNMGSHKNVIFTDLFGASPTNAAAMTLVDIDAAVIAGVNLPM
                     IAEILSVRNQYLDFDDLLNDIVAKGKDSIRIITKRMISEG"
     gene            complement(23049..23759)
                     /locus_tag="PLL88_04870"
     CDS             complement(23049..23759)
                     /locus_tag="PLL88_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ00930.1"
                     /translation="MKETIPIYQQIIAYIKEYIENGIYQKGNIIPSEQEFCNQFNTSR
                     MTVRKAIDELVNDGYLYRIQGRGTFVSHWVYRKSLEVLGFSYHMKQLGYTPTSKVLSF
                     EIKTPLISVAEKLKILPTENVYYLSRVRLADLEPIAIENVCLPEKRFPGLEMYSFEEN
                     SLYEILKTKYNAEVVLKQEKINAVMVEGNEAKILFEKDKGVGMRMNGVDFDANMTPVA
                     YADSIYHGTKYIFDVIFR"
BASE COUNT         6822 a         5992 c         5055 g         6491 t
ORIGIN      
        1 gccctcgtcc gaaggcatca ttgctgaagt gcgcgacaac ccgaacgcca ttggttatga
       61 tgggctgggt tacgtcacaa acgaagtgaa aatgatcgcg ctcgacaacg gcgtgggata
      121 catcctgccc tccgaagaga cggtcagcaa tcacacctat cccatcgcgc gccagcttta
      181 catgtacacc aacggagaac cgcagggcat agtgaagaat tatattgaat ggatacgctc
      241 tagtgaagca caacagtatg tgctcgatct aggttttatt ccgtataatc aaggcgagtg
      301 agaaagcaca tgaaagatcg attggtcaca cgtatcatcg aaaccgccgg gttttccagc
      361 atcatcatcg tgttattgat ctttctgttc ttactgcgcg aagggatgcc agcgctgaat
      421 gaagtagcgc cgggagatct gtttaccaca cgttggtacc ccatcgaggg gtacttcggc
      481 atcttgccac tgttctcggg ctcgctgctg gtgaccctct tttccatctt gatggctata
      541 ccttttgggg tgggcacggc ggtgtacatc gccgaggtcg ccccacgcag ggtgaaagag
      601 atccttaaac ccacgatcga gatcctggcg ggcatcccct cggtggtact gggattcatc
      661 ggcatcgtgt tcctctcccc tgccctgcgc gagtggctga acctgcccac cgggctcacc
      721 gctttcaccg gagcagtgct gctggcgctc atctctatcc ccaccatcgt gtcggtgagc
      781 gaagacgcgc tgaatgcagt gccgcaatcg tatcgcgagg cgtcgtatgc gctgggcatc
      841 acccgctggc aaaccatctg gcgcgtgacc ctgccagccg cccgcagcgg ggtaatcaca
      901 gcggtgatgc tgggcatcag ccgcaccctg ggcgagacca tggctgtgat gatggtgacc
      961 gggaatgcgc ccacgatctt caagggcatc cagtggctgt tcaaacccac ccgcaccatg
     1021 actgctacca tcgcttcgga aatgggtgag gttgccagcg gcagcaccca ttatcatgtg
     1081 ctgttcttca tcggcatcgt actgttcctg ttctcgctga tcatcaatat catttccaac
     1141 cacttcagcg agaagaatcg caaagataag gaacaggtgg tttcatgaac ataccaggca
     1201 gccgcgattc gctgattctg cgcagggtga aggactcttt ggggtacata ctgatccaca
     1261 tcatcagctt tttcaccatt ctgcctatcc tgggcatagc gctgtacatc atcgtgaacg
     1321 gggctcccac cctgacgtgg gaattcctga ccagcatgcc aaccaacggc atgaaagccg
     1381 gcggcatcct gccagccatc gtcggcacgc tctacctcac cataggcact gccatctttt
     1441 cggtgccgct gggggtggca gccgggatct acctggcaga atacgcaccg cgcaaccact
     1501 ggacgcgcct gatccgcatc gccatcatca gcctggcagg catcccatca gtggtatacg
     1561 ggctgttcgg gttgggcatg tttgtgctgt tcttgaaatt cggcaacagc atcctttcag
     1621 ccagtttgac gctggcaatc atgaccctgc ccaccatcat cagcaccacc gaagaagcca
     1681 tgcgggtagt gccggagcgc ttcagggtgg tgtgtggctc gctgggcgcc agcaagtggc
     1741 agggcattca gcgcatcatc ctgccagaag ccatgccggg catcctgacc ggggtgattc
     1801 tgggagtgga gcgggcagcc ggagagaccg cgcccatcct ttttacgggt gccactttct
     1861 ttttgccgca tttgccaacc tcaccgctgg atgctaccat ggcattaccc tatcacatct
     1921 ttgtcatctc cacgcaggtg ccgggcatgc cgctgcagat ccaattcgga tcagtgctgg
     1981 tgttgctgat tttcgtgttg ttgatgaacg tctgggcaac cgtcatccgc aaccgcgcgc
     2041 gcagaaggag gcaatggtga tatgagcgac cccaaaattt ccattcgcaa gtattgcctg
     2101 cgttattccg acggggtaga agcgctcaag aatatcgacc tgcaaattca ccctcatgcc
     2161 atcaacgtgc tgttcggtcc ggcaggggga gggaaatcct cgctgctgcg ctccatcaac
     2221 cgcctgatcg acatggcgga tgtagaagaa accagtgggg agatcctctt caacggcgag
     2281 aatatcctca gccaagacac ggaagtgccg tggctgaggc gcaagatcgg catggtcttt
     2341 tcgcgtccca tcccgctgcc caagagcgtt tttgaaaatg tggctttcgg actggaggtg
     2401 gcgggagaaa aggatgaaaa gctcatccgc caaacggtgg agcaggcgct caaacaagcc
     2461 tacctgtggg aagaggtcta cgaccggctg gacgacccgg gcacttcgct ttcgggcggg
     2521 cagcaacaac gcttgtgtct ggcgcgcatc ctggcattac aaccagaagt gatcatgctg
     2581 gatgaaccca cctcggcgct cgacccggtg accaccgcgc gcatcgagag ttttctgcag
     2641 gagatcaagg aacgcatcac catcatcatc gccccgcaca acatgtcgca atcggcgcgt
     2701 gtggcagatt acgcttcatt tttcctgcag ggcgagctgg tagaatttgg agagggttcg
     2761 aagctgttcg tgaacccaca ggacaagcgc actcaagatt acgtggaggg caggttcggg
     2821 taagttcgag ttgacaaaag ttgacaaaac ttgacattgc gcatcattgc aattgcaagg
     2881 gttcgtttca ttcgttccaa aggaacgaac cagaagaata acactgacct ttaggaggaa
     2941 agcatgtttg agaaagcaaa gaaaacgatg ccgctggcga acgccattct gacaggcgac
     3001 agcaaacaag tgcgacgcca tcttgaagca ggggtggacc cgaaccaacc catccccggt
     3061 gaagatgggt gcccactgca ttttgctgcc aacagtcgtg ccgacatcat ccggctactc
     3121 atcgatcacg gtgccgacgt caatgtgagg gatggcaacg ggaaaacgcc cctgcactgc
     3181 gctgccgtga cagccaatct ggagaacatg cgcgcgctgc tggagaatgg cgcagatgtg
     3241 aacgcggcag acaacgatga caacaccccc ctgatgctcg cccggcgcgg cacgccagcg
     3301 gcggcttttt ttgccaacct gggggtacct tcgaacagcg aggacgactt gaagcaaaag
     3361 aaagcggcag acctgctgct agaatacggg gcaaaagaat aaagagccaa ttcagcgggt
     3421 gcgttggaaa cgcgcccgtc ctaccttatt tatttgtgag gaaattatct tggtgggtta
     3481 ggaacgcgcc ccacgattgg gattcctctt tgcgacaacc tccgcggatg ccaccgaaat
     3541 ggacagatcc ttcgtcgttt cactcctcag gatgactgcg gggaaaaaga agagagttgg
     3601 cgcgttagga gcgcgcccta cgacttgttt tggaaacaga caggaaacgg tgagattcct
     3661 cggtcgcgat gctccctctg aatgacattg cggggagaaa atgggaaaag caggttgact
     3721 ggtttaatat gtaaaatgga gagatacttt caatcccgct ttttgtattg ctaactcttg
     3781 acatttaaga cggtgtctac gactgccgat ctttttggga atgcacccca ccggcaatca
     3841 gcggaacgca cattcttccc cacaaccctc cacaaacctg cctgatcgat ggaatgttgc
     3901 aaaaaacttg agcaaaaatg gatatttcag tcccggagtt tctcgcctcg ttccattttc
     3961 tcccacttca gtgcgaccgc tgctaacagc ataaaaggca aaaaactaat aatttttcat
     4021 tttcacaatc aatcacaaac aaaaatgacc attcactttt ctcaatcaag tgacatcttc
     4081 aaacattgct aaacctcgaa agccattaca cgatttttgc agaaacccat gcccaaattt
     4141 cacgaaacat tccctgcaat tctgtgaaaa gtgcccttta aagaagggta cagcgaacgg
     4201 tactctgaat aaattgtttc atagatcctg gctgcactgc cgggtacaac acgatctctg
     4261 accgtgatcg tattggcaca cgcctgttcc accgagtcaa acgcacctgt tcccgtggca
     4321 gcaagtaatg ctgctccaaa agcggcgcct tcttccgaat tgatcgtgac aatttcagcg
     4381 ttgagaacat ctgcgataat ctgccgccat aaaaggctct tcgagccacc tcccgtaatc
     4441 cgaacctgtt cgattcttgg cagacccgct tcattcatca gttcgaaacc atcgcgcaga
     4501 ccaaaagcaa ccccttcaag gacagctcgt gtgaaatgtc gaatcgtatg gcgcaccgta
     4561 atcccgataa aaccgccacg agccagagga tcgggataag gagtccgctc acccgtaaga
     4621 taaggcaaaa atagcaatcc accactgccg ggctcaatat cctcggctcc agcaaccagg
     4681 tcatcaaaat ttgtttcttt cctgaacgta tccttgaacc atctgaggct gccagcagca
     4741 gagagcatca cccccatcaa atgccatttt cccggaacga catggcagaa tgcgtgcaac
     4801 cgtccgtcag gttctatacg cggagagtcc gtcgatgcaa agacgacgcc cgacgtcccc
     4861 aaactgatcg acaggattcc ttcttttact gcgccagtcc ccaccgcaga agccgcctgg
     4921 tcaccaccgc cgccaaaaac agggatgccc tgcatcaacc caacttccgc tcctgcctgt
     4981 ttggtcagcc agcctgtgat ttgcgtgccc tcaaaagttg gtggaaacca cgcaggatca
     5041 aggcctagtc ttccaatcag atcgtaagac caattcctct ccttcagatc aaagagaact
     5101 gtccccgatc catctgctcg atccaaggca aattccccgg taagtttata ccggacaaaa
     5161 tccttcggaa gcaaaaggtg cctgatcttt tcaaatagtt gtggctcttg ctctttaacc
     5221 catactattt taggggcagt gaaaccggtc agcacatcat ttccagtggt cttgataaat
     5281 tcttcttttc caattaaatc gcgaatttca tcacattgct gctgagtgcg ttgatcattc
     5341 catagaatag ccggtcttag aaccgtcccc ttttcatcga gaataaccaa gccgtgcatc
     5401 tgaccagtca atccaatccc ttttacctga aaggacttca atcctgcttg tgcaatgcag
     5461 ccacgaatac ttttctttgt tgcctcccac cacagatcag gattttgttc acaccacaaa
     5521 ggaaatggcg tctcatacga gtattccgat gaggatactg caagtatttt tcccagggaa
     5581 tcaatggcaa tcgttttcgt tgacgttgtt gatatatcaa ttcctaaata gatatccatg
     5641 tgcacattcc tagaacaatt tttcgcggca aatttttgtc atttcatcat ggagaaaaaa
     5701 atctttcaga tcgctcaaag aaaattcaac aaagggatcc tcaaccgctc tccaaatttc
     5761 tcgatgacaa atttccttgc cggattcaac atccgatagg ggacccaacg tttccagttc
     5821 aatgaatttc gaattgcagt aacactggct ggttgcacca taatcaatgc attgcctcgt
     5881 ggggtaattg ctcgcatatt ttacaaatag cagattgttg cgataataag ccatccattg
     5941 ataggggttt gccattccca ctttcaatgc tttatcaata ggcaaagcat ggatggcgat
     6001 aaactggtta tcccagttga accttggatc tttgacatcg gaatagggcc ataaagcaat
     6061 cgaacggttg ggtaacagag ggttctcatc catcccaccc atctgttgag gaagaattgc
     6121 cgttccaccc aatttcaatt gagtaattgc ccagggggca caacgaaacg gtttgtcctt
     6181 tgcattccga atgtggtggt caacaataag aatattctcg aactctgtag gcataatttg
     6241 catacttttc tgaatgccaa gggttgaatc cacttcttga aggagttcta ttgagtcgga
     6301 tgtttcatta acgattattg gttgggaatc aggaacatag gtaagcgagg gatcctccgg
     6361 cccgcaccat aagcggtgtc ccccataaaa atagaacttg ccttttccag gatgctccag
     6421 atattgaccg ggaacttccg caaagatgtt ttctccgcca ttcaaacaaa atgaaaggat
     6481 tcttggtcca accgatttag aaatcaataa ttgtatatga ccaatgttga tttcaagaca
     6541 atcgagatca aggaaccttt tttctttcct atccataaca aagacctatc gaactcccaa
     6601 aagcacttcg ttcgtcaatt gatcaagcaa ttcataccgc aatcctttta cgcttctgga
     6661 tctgcgatca aatgggtaga ttttcaacgc ctgtgctttc gcctgcgagt atggaccttt
     6721 gaattgcggc agctcgctat ccattttatt cagttcaccc agcaaaccct ggatctccgc
     6781 atcatgattg aattgatcga ctttctcttt caatatcagg taggtgcgca tgcaaccgcg
     6841 tgcaaaatcc ttgacgcctt cctcatcttc agttcgatac gcatgggcat caaaatgccg
     6901 gctgcccgcg tagtgatggt cttccaaaag tttgacaagg aaaaaagcac ttttaagatt
     6961 caccgcagcg aatcgaaagt cctgatcgta acggccaaat acctgatcgt tgagatctat
     7021 gtggaacaac tttccggcat caatcgcctg cgccacccca tgagaaaaat taagccctgc
     7081 catctgttca tgtgcaactt ccggatttac gccaaccatt tccgggtgat ccagggtgct
     7141 gatgaatccg agcatatgtc cggttgtggc attatataaa tctccccgcg gttcgttcgg
     7201 tttcgcctca agggcaaatt tcaccggata tcgttgatct agtgcatatt cacaaagaaa
     7261 attgaccgct cctcgcatgc gtttcatgct ctcaacagga tcttttgaag catctgtttc
     7321 cgttccttcc cttccgcccc aaaacacaaa aacttccgcc cccatctcaa ccccgaggtc
     7381 cattgacttc atcgtctttt ggagagcgta attccgcaca taggcatcat tggatgtaaa
     7441 agcgccatcg cgaaagattg gatcataaaa aagattggta gtagccatcg gcaccaccaa
     7501 gccggtatct tttagagctt tcttaaaatt ctttttgatg gtttcaattt cttccggcgt
     7561 agaatcaatt ggaatgagat cgttatcgtg aaaattcacg ccataagctc caacctccgc
     7621 cagcaaataa accagatcaa gcggagattt tggatctcgc acctcagcgc caaatgggtc
     7681 accgcccctg tttccaactg tccagagccc aaaagtaaat ttatgctctg gtttcggatc
     7741 gtattttgtg gtcatggcta tctccttttc ctttaccaaa tttgaaaaca agttctttat
     7801 caacgcgagc aaaatgaatt tctttcaatt caatttgaaa accgttgata atggtttggg
     7861 cgctcccaaa tcctgcccgc gtttgccagg ggaacacccg ccaatttcca aacgaaactc
     7921 gcctggttcc agggttaact ttccatcgtc atcgtaaaaa gacatcatct caggcgttac
     7981 ctgaaattcc actttcttct tctcccccgg aaaaagccca atccgttcaa atccaatcag
     8041 gcgatgaaga ggaacgatcg ttgaagcttc gagatctgac agataaaatt gaacaacttc
     8101 cagagcagat cgatcgccac aattgtggag ttcaaaactc aagtggagcg aatctccaag
     8161 attgatgttc tggtcttcca aatgaagatc ccgatactca aatttgctgt agctcaatcc
     8221 aaaaccaaag gggtaaagcg gctcttcctt tatgtaccga tatgtccttc tatccatgct
     8281 atagtcatca aaggaaggaa tttgatttaa tgaatggggg aaggttaagg gcaattttcc
     8341 tgagggcgaa acatcgccga acaatacgtt cgcaaccgca tttcctccct ccatacccgg
     8401 ataccagata aaaagaatag catccaccag atcctctatt tcaccaaggg caatcgggct
     8461 gccacccgtc aaaacaagca caatcttggt tcctgacatc gccagttctt taatgtagct
     8521 gatttggctg acaggaaggg aaatactgct acgatctcca ttttgaggag agagcagaga
     8581 ttcgccttct tcgccctcca ggaaagaaga ggaacctcca caaacgattg ccagatcaga
     8641 tgacaatgcc atccccggag cccaagtctc tttgatctct ctggggtgtt ttaacatcgc
     8701 accggtagta tattccatcc ccatcccttc aggaatccgt cctgtaatcc cttcgagcaa
     8761 tgtaaccatc cgttcattaa atccgtaata attgcccaaa agaacttctg tactggtcgc
     8821 atttggaccc gtcacaaaaa ttttcctaac ggaaggttga atgggaagaa tgttatcttt
     8881 gttcttcaat aagacaaatg attccgtcgc gatctgataa gcaagctctc tgtgcttttc
     8941 acaaccaata acactttctg gaatcgacgc aaagggaaca tcttcaggag ggtcaaacat
     9001 cccaagtttg aatcgggtca gcaaaacgcg ttccagagca caatctacat catcttccga
     9061 aagcagccct ctttggatag cctcaggaat ctcgtcaaaa atatggtcac aacccaggtc
     9121 acaaccatgt ttcaacgcca aagcagcaga ttcagccgca tctttcgtaa ccttgtgact
     9181 tagatagaaa tcggacaatg ccccacaatc tgacacaaaa tgaccttgaa atttccattc
     9241 cccacgaaga atatcttcta tcagaagctt actggcacaa cagacctccc ccagcgttcg
     9301 attataagct cccattaccg cttcaacatg tgcatcctgc accagttttt taaaagctgg
     9361 caggtaggta tcgtataact cacgcttggt taccattgca ttgaaagtat gccgttctct
     9421 ctccgggcct gaatgaactg cataatgttt cgcacaggca gcggtcttca gatatttggg
     9481 atcatttccc tgcatacctt tcacaaaagc agaagccaac tcgccggtga ggaatgggtc
     9541 ttccccccac gtttcctgcc ctctacccca acgaggatca cgaaagatgt taacattcgg
     9601 cgaccagaaa ttcaaaccct gatattgatc cgtatatcca tttctccgca aagcctggtg
     9661 gtacttagca cgcccctcgt cgctgatagc agtggccact tcatagacca tgtctttgtc
     9721 ccatgtggca gccattccaa ttgcctgcgg aaaaacggtg gcacgaccgt tgcgcgctac
     9781 tccatgcaat gcctcactcc aatagttata agcagggatg cccagacggg gaatgtcctt
     9841 tcccgtatag cttatcagtc caattttttc atcaagggtc agcaacttca tcaattcgtt
     9901 tattcgatca gtaaatgaca acgaggtatc aagataggat tgctgttctg gcttaccttt
     9961 cacttccata aaagcacctc ttttttataa atttcctcct gatggttctg cgaagatgtc
    10021 ctttcaacct tcacaggatc aagaacaatt ccacatttcc tattgcagat aaaatgaaga
    10081 tccaattttt gactttgcaa atggcatagc tctccctatc tatcccttca ctgcaccggc
    10141 tgtcaaacct gaaatgatct gtttctcggc aaaaaaatag aaaatgattg taggaatggc
    10201 agacaatgca acaaaggctg aaattaatgc caaatcttgc ccatattgac cttgaaattg
    10261 catcgtgcct aaaggcaaag tccataatcg attactattc aaaatgacaa gcggaaccag
    10321 taaatcattc caactggcaa tcatgaccag tgaaccaaca gcagttaacg atggtttcac
    10381 caatggcaac ataatccgcc agaagaaatc aaaattcgaa catccatcga tatacgctgc
    10441 atcttgaagt tcagagggta cggcagtgaa aaaacccttc aagatcaaaa tattcccgga
    10501 taattgaaaa gcaatttgga taaaaatgac gccccacagt gtatcaatca taggaatccc
    10561 tatttgttct atttgcctca acacaaaata cacaggcaaa atagccacat taattggaaa
    10621 catcagacca accgtaaata gattggagaa aaaatttttt cctcgaaact gtaatcgggc
    10681 aaaaacaaag gcgaccaatg aacagacgca aacagttccc atggtcgtag ctgtcatcac
    10741 gatcaaagaa ttttttaaca tgttccaaaa cgagggcgtt ttcagtattt gcatgagatt
    10801 gccccaataa ggagggtttg gaatggaata gggatgcgaa tacatctgac ccgttgtttt
    10861 cacactgcca aaaagcaata tcacaagggg gataaagatc atgatcgaag caaaaataag
    10921 aaaagcataa acaaccaatc gttgaggagc ctttttcatc catcttggaa gggcaatata
    10981 tttactcacg tggctattcc tttctacaac ccactgaggt aatccggctt tcgcgtgagc
    11041 gcctggtaaa gcaatgaaaa gatcatacaa aggatgaaca taatgatggc aacggctgcc
    11101 ccgtaaccaa gctgaaagcg aacaaagccg aagcgataca tatacgtcgc catggtttcg
    11161 cttgcattga ccggccctcc ccttgtcatg acccagatca aaacaaattg ttgaatcgac
    11221 cccaagacag acatatacac ggaggtctta atggtgcttc ccaaaagagg cagggtaata
    11281 taaaagaaat tttggattgc gttagcacca tcaatacgtg cggcttcctc aatttctctt
    11341 ggtatatttt gaaggccggt aagatacaga agcatatgga aaccaaaata tttccaggtt
    11401 aacgttataa ataccgcgta gatcacgaca ctcatatctg ccagccaggc aaccggctgc
    11461 aaaccgagcc atacgaaaat ggcattaaag aatccccgtt ctggatctgc attatacagt
    11521 agcatccaca taattcccgt gttgacttcg gaaagcacgt aaggcaggaa aaaaaccgtt
    11581 cgaaaaaacg atcgtccggg tagatcacgc cccaccagaa ccgccaagac caatgccatg
    11641 ggcagttgca accctaaaga cagggtaata atgagcaaga catttttgat tgattttatg
    11701 aatacttcat cgctgaatat cttcttataa tttcccaagc caatataatc cactgccggt
    11761 ccaaaacctt tccagtcata catactgtaa taaaccgatc ggaaaattgg atagattaca
    11821 aagagaagaa agagaaccaa tgctggcagc aaaaacaaga taacaaccaa ccgattttga
    11881 aaccgctggt tttttggggc gttctcttga acactgtgtt tgtcatgaat gaattgagac
    11941 aattgcatgt ttcggtatcc ttattccctc acacaaaaat tgtttaagaa atttctcgat
    12001 atccctctcc gatttatgaa acaaaaaata atcaaattca cgtattcagt atcattgaaa
    12061 ccattcgttt ttcaaagaca ggtgcgttcc tgtttttttt accgaccctc gcgcacctgt
    12121 cttgatcctg cttggaaatt atctaatcaa atcttcggca ccggcctcaa tttcagccgc
    12181 cacctcttcg ggtgttgcca ttccggcaaa caacttttgg actgcatcat taatgatgcc
    12241 gcccatcgca gaaggcaaaa tctgatcata gtaaagctgg aagtatttcg cctgtgcaaa
    12301 gctttgctgt acaagcaaca ggttcggatc agtcaaaccg ctctcagcac ctttcaccac
    12361 aggaatgccg gttcctgatg ctgcgatgat ggtctggttt tgctggctgg tcaggtattt
    12421 cacaaagtcg atggcttccg gttcagcatc ctttccaatg gcaaatccat ttccaccgcc
    12481 aatcacatcg gtaagatcac ccgcaccacc ttcgacagca gggaagttga acattccgag
    12541 gttttggacg cctgccagat cagcgctgtt cgctgcctgc gttgacggag cccactgccc
    12601 agaaagttcc atggcagccc taccatttcc aaaagttgcc tgcatttcat catgcgtggc
    12661 acccagataa ccgtcttgga atggttgcat gtcgatcagc cttttgagtt ctacccctgc
    12721 ttgtacgaaa gcaggatcag tgaaagagcc cgtacggtcg gctgccgcag tgaatgctgc
    12781 ctcaccccca atgcgcgtgg caagatatga ccagatatgc ataccggtcc aagtatctcc
    12841 ctcacccaat gaaatcgggg tgatcccagc atccttcaat ttctgaacgc attcaaggaa
    12901 ttcagaccag gttgtaggtg ttgcagtaat tccagcctga gcaaaaaggt ctttgttata
    12961 ccaccatcca accatgccca tatcccatgg cacaccgtag ttcttgccat ctgagctgaa
    13021 aactgccaaa gcaccggggg caaacgtact tcgccaggca ccgccatcgg catcaagatc
    13081 tgctgtaata tctttcaata gcccagcatc aatttgttcc tttaaagtac ccccacccca
    13141 actctggaaa atatcaggca cttcaccgga ttgcaacact gtggtgagct tggtcttgaa
    13201 tgcttcgttt tcaagaaccg tgatttcaat gttcacattg ggatgtgcag ccatgtagtc
    13261 atcagccatc ttttgccaat cagacagtgc cggatctttt gtggaaatat gccaccatgt
    13321 aatggtcacc tgttgctcag attcagcggg ggcttccgct gacagtccct ctccttctgc
    13381 aggtgctgtt gtcttcgcgc acgcagtgaa caggaaaagg gcaatgaaca acacactgat
    13441 catcgagaat atctttcgat tcatgtcttt cctccacttt tgacatcaaa ggatataata
    13501 tgatcgtgtg gagaacacct ccacacaact gtacaggtct tcttgaaaca cgcaagagtt
    13561 taagagaaga cctgttttaa ttatttatgc gatttacccc acctcctctc acaaccatgg
    13621 tgttcaacca gtagcatcag aaccgtcgct ctaccgacat caatttttcc ccacatatgg
    13681 ccggcatgaa ttacgaacta ccagttgcgt ttttattttc agagtttgat tctcctcagg
    13741 aatttcatta atgatcctga tcaacatttg tgtggctgca taccccattt cggagatgaa
    13801 ttgatctact gtggtgagac ccagatattt cgactcgggg atgttatcaa agccaaccaa
    13861 tgaaaaatct ccgggaatat gcaattccat ttcctcagca atctgataaa cccccatagc
    13921 catctgatca ttggatgcaa agatagcggt gggtcggtct tcaagtaaaa gcaatttctg
    13981 tcccccctca atgccggttt tcgtcgtgta gtcgcctgat acaatgagct gttcatcaac
    14041 aggaatccct gcttcctcaa gggcattgcg atatcccgat aaacgttgat tcgcactctc
    14101 caattccaca cgtccgccaa tgaacccaat cctttgatgc cctagcccta ttagatattc
    14161 cactgcttcc atggaacctt cgtaatttgt agcatgcaca gacggataac tcggatcact
    14221 tgttaccgga tcgatggaga caattggttt atcaatgtta tattcagaag taaccggcgc
    14281 aactacaata attccatcag aaatactttt tgccaaaagg gtaatatatt tttgttcaga
    14341 tgtgatatgg ccccgtttgc gcacatcacc agaagtgtaa accaagagat caaactcaga
    14401 ttccgcaata gcctggttca cccctttcat gacctcgatt gcatagggat atgcaatatc
    14461 tggcataatc aagccaatca gattcttttt tgagctgcgc atactgcgcg ctgcaaggtt
    14521 tgtgctataa cccaggtcat caataatttt caaaacatga ttcttcgttg ctttggcaac
    14581 atccacttta ccgttgagca cccgagaaac agtagaaact gacacgccgg attttttagc
    14641 gacatcgcgt attgtgatgg atcgtttgtt ttgttccacg ttcatgttcc ccaatcaccg
    14701 gtaacgttac cggtaacatt accgatatta ttacataaat cagatcaaaa gtcaaccctc
    14761 aagtagatca actatgggaa ctagtaaagt aacttttcta tatgagtcaa cgttactacc
    14821 aaagttactt tttctattga gtcattatcg tcctcattca ttttgcttta agggaccagt
    14881 gtgcatcttt ataaaacaag gtgatggaga atccagcccg tttcgctgtt gaagtctgca
    14941 ttaatggaat ttcaccccat tttttgggaa ttcgccccac cgacgatcag cggaacgcac
    15001 attcttcccc acttcattcc acccaatagc ctggtagaca ggaatatctc aaaaaagttg
    15061 gggaaatcac cctcttttgt ctaaaagaaa tcttgccgca taccactttt ccccacccca
    15121 ttatttaaga gatcataaaa agggacagtt ccctgttcaa gaaacagatc tgctcattgc
    15181 accaactaca tcaacaggtc cacggctttg gggtcagaat gcttgtggat tgttcttggc
    15241 ttttttccgc gaccatgctc tgcgataagg gcggtcatgc gagagtgcat cccacacgtc
    15301 agccggtgca aacagccgga cttccattaa atctgctttc cttccaaccc ctgaggataa
    15361 ccggttccat cccagcgttc atgatgctcg tacggaatat gataataaaa aaccaattac
    15421 tcaacgatat tcctttgtct ttccccttaa gggcaagtaa ggaaaatcta cggattcatc
    15481 cctgcggcag gttgaggaaa cgattctgaa ttggagcggt tcttcagccg taacatttct
    15541 accgcgtgat gtgaggggag gaaatgaata gagcaagttg gtttgatata tggatatatc
    15601 gttgcgattt attttgaaaa tagacaggaa atggcgagtt tcctcggtcg cgatgttctt
    15661 gcaggataat agagaaaaga atttattgga aaatactttt cttttacatt gttatgaata
    15721 ttatattatg attagccatt gacatcaatc ttatcttatg ataagatgca aacgttgagg
    15781 gaatgctctc aacatccaat gaggtaaaca atgggcaaaa cctcaacaaa agatattcag
    15841 gattttttat gccaatgtag agaaataatt caaagcaata aggttgtccc tagcaatttc
    15901 tggttcattt caaagaaaga aaatagagac acattgcttg cattaggatt ttctttacaa
    15961 gatataaaat atgaaatttc gaatttatcc cttgataatt attgcgaagg accgtgtgat
    16021 gcacacgagc acaaagacca agtttggata tttggaaaaa tcatgaataa tcgagaaatc
    16081 tatatcaagt tatgcgtttc agattttgac aatgcaggca ataaaattaa aacattatat
    16141 tgtttatcat tccattttgc acaaaagcca atgaatcatc catataagcg gaattgaagg
    16201 aaaagaaaat ggaaaagtat tgccctatct gtggaaaacc tactgagtgg aaggaaaaga
    16261 tcagtagaga tgaattcgat gtacgagggg aaaaaatctt tgtagaattc ccactatttt
    16321 cgtgctccat ttgtaatact gaatttgaag atttgaattc agagattgat ccatatgcac
    16381 tcgcttatga ggaatatcgc aatcgtaaag gaatggttca tcctaaccaa atcaaggagt
    16441 tccgaaggaa atatgatctt actcaaaaag agttgagtga tctactcggg attggaggaa
    16501 ttacattaag caggtatgaa aatggcgcgc tgcaagatga agcacacgat caattattaa
    16561 aattcatttt tgaaccggtc aatctctata aagccattcg ggaaaaagaa agattattct
    16621 ccgcagagaa gagaaatagg ttattaccca aactggaaga agaagctggt ataagttctt
    16681 atagactatt aaattcagaa aaacatacgg acatctatac tgggaacact gaatttaact
    16741 ttcagaaagc tgttaatcta attaaagctt tcacatccaa tcaatctatt ttcaaaagca
    16801 aactttttaa actaatattt tatgctgact tcagccactt taaagagcat ggcgtttcaa
    16861 ttacgggctt acaatatgca cacctccctt acggtcctgt accgaacaac tacaattcct
    16921 taattggtgt aatcatgaat gtggattcat cgatatacat tgaggaaagg attgctaatg
    16981 attatcctgg tgaagtcatc cttagttcaa cgcctgctgc aaatattttc tcaaaagacg
    17041 aaatagagac tattcgaaaa atccaagata agtttgctca atttacgtgt aagggaattg
    17101 aagagtactc acatgaagaa gtcggctaca aaaagactca aaatggggag ttgatttcct
    17161 acgaatatgc taaagatcta caaatatagg agtaaattat gtcgggaaaa aatcaacatg
    17221 tagtaccaac tggaaatgga aaatggggtg tgcgtggagc tggaaatcaa cggcttactc
    17281 acataaccaa tacacaagct gaggcaattg aaattggtaa agaaattgcg aaaaatcaag
    17341 actcagagtt actcattcat ggcagaaatg gtcaaattcg tagtaaagat agctatggga
    17401 atgacgattg tcctccacgc gacaaggaac attaatcctt ttctgcctct ctaacaacca
    17461 gataatccac tgcaacagat tcggaaaggg attagaaaat ggagagattc ctcggtcgcg
    17521 atgctcactc ggaatgatag ccagaaatgg aagttcttgt tgatcttaag tgagcatagt
    17581 caacacagca aacacttttt gatccaccat aaaagtcaat tcccaatacg cgcctattat
    17641 ttttacaggc tttcctcgta aactgtcaca cctttgataa tcgttctttt gacattaata
    17701 ttttcatcaa aaaggatgat atctgcatct tttccaattt caatgctacc ttttgtttct
    17761 gacaaaccaa tttgtctggc agggttcaaa cttgccatcc ttacagcagt gggaagatca
    17821 atccctgtca tttcgacgag gtttcttacg gcacgatcca aactgagcgt tgacccagcc
    17881 agggttccat ccagaaggcg cacttcatta ttttttattg aaacttttgt gcccatgtaa
    17941 tcatacacac catctggcat tccattcgct ttgatcgaat cagagatcaa aaccactttt
    18001 tctttccctt tcattttcat gatgatctgc atggctgcgg gatgaaggtg aatgccatca
    18061 cagatcattt cacaggtcac tctatcatcc agcagacaag ctccaacaat gccaggttcc
    18121 cgatgatcga agctgcgcat gccgttgaac atgtgagtgg cttcgcttat tccattttca
    18181 atgccgttca tcatttgttc ataggttgca taactatgcc ccgccgacac tgtaattcca
    18241 tgatcaatca acaacgtgat cgcttctttt gatcgttcca gttcaggagc cagtgcaaca
    18301 attctcacca atccctttcc acgacgaaca tagttcttca attcatccag atggataccc
    18361 gtaatatact tctcactctg cgctcctttt ttcttgattg aaaaataggg accttccaaa
    18421 tacattccta acatttggga agccggagaa tctgccgtcg ttgaatattc aaccgcattt
    18481 tcgatggcac gaactgttgc atcgcaggac gccgtcatag tggttgccaa aaatcctgtc
    18541 acaccagttt tcatatggaa actggcgatg acttccaggg cgttgatcgt tgcatccatc
    18601 gtatcaaaac cggagttacc gtgattatgg acatcaataa agcccggcga gagatagagg
    18661 ccccttccgt cgatcaccat acaatcggcg atatcaacat tttcattcgt gatcgcagaa
    18721 attttcccat cctgaacaag aagtcgatta tcaaggagaa tctggtcttt tagaataatt
    18781 tttacatttt ttattaattg attcacattc ttctccacac gtccaacata tagagcagtt
    18841 cgggatattt cgaaatcagt tgcatataga tcttctgatt gtattcatcc gttccatcaa
    18901 gatttccact catcaggatt gccggttcat ttcctttttg gagcaatatc ccaattattt
    18961 cgacaacgag agcctgcaaa atgattgctc ctgccacagt cgatgtggaa ccgatactat
    19021 agggtttgcc tctcacttcg atcgcggcat ctccggttac gccacaatta tcgagcacat
    19081 aatcgcatac ttcgtacaat tttttaccac tcggacatct tgaagtgaca ctcttgctgt
    19141 gttcaagatt ggtaagtgca ataaccagca cgtctgcttt ctttgcctcc tctgccattt
    19201 ccacgggcac gggattcctt cctgaatttg aaattacgat aagcgcatcc ttctttcgaa
    19261 tggcattcgc ttcataaaca atcttcgcat atccgggcat acgttcaatt tgcgtgcttt
    19321 ttcctcttcc ctggatctcc gtcaattctg gttcaaggat cgctcttacc tgcataagtc
    19381 cccctgctct acccgcaatt tcttcactga gcatatgaga atgacctgca ccgaacgtat
    19441 aaacaattcc cccatttatt aaaatatcgc ttaatttttt tgcgatggta ctgatcgact
    19501 cgctttgggt ttctctaatt ttatgtaaaa tttcctcaat cttatcgaaa aagatttcct
    19561 gcatcgttgt atcctccgaa ttcattcatc cataaaatca acgtttgata agcaagccgg
    19621 cttcatggca ttcacaaact tgcccgtgat cttttctggc atggtgatag cagctccaat
    19681 agttatcata tctgctccac attgcatggc tttcaccacc tggtttgcct ctgaaatcct
    19741 tccttcagca ttgatcttta ccctgacatt ttctctcaac gcttgaatca atccaaagtc
    19801 gggacccaag tcagatgcct tatccaatga agagctgctt tgcatcaaca aacgatattt
    19861 ctccgggtca accttcagac tctccggtgt atatccggaa agcgtggtag agacgatatc
    19921 gacacccatc tccgctgctt ttacaccttc ctccagcgta gataaatcgg ccataatgag
    19981 aacagcaggg tagtgtttcc tgatttgctc aactatttta tgaatatcgt catatgttct
    20041 acccctgggg gtacaatcca ttcccaagat atcggcacct gcttcgatca cctctgccgc
    20101 ggattcaaaa gtgggggtga tgtagacctt attgattgac aaaggctcat catccattaa
    20161 tttattgatt ccaacaacag gtaaatttgt aatctctcta acagctttga cattatctgc
    20221 ccaacacgcc cggatgccgc cagcaccacc ttgtatggca cacatagcca tcttttgcat
    20281 ataatcggct ccatataagg gggagctcgg gaaagcctga catgaaacaa tcaatttatt
    20341 ttgaagattt gatagaatag tattcatatg aactccaaag tttttccgta tttaaatctg
    20401 cggggttcat ctaccagtgt ctgagcccca tgaaggggct cagacatgac gcttaaactg
    20461 ccagcacttt cgtgaaatac cctacaatgg caacagcaat gaaaaccagg aacagccatc
    20521 cgatcttcat tttcttttct gccatcaggt aataggtagc cagacccaac aaaaccgtga
    20581 tcaggttcgg gaataatccg ttcaatgttt ggttgaggtc aatgaccata tcaccctgtg
    20641 taaaggttaa gccggttttc acattaacat acatggatgt gatcgcccct acaaccacca
    20701 aaccaatgat gttcatagcc tgtgtgatct tatctttgat gccaccagat aagaccaatt
    20761 gggctgattc catacccatg ctgtacccgc tcttgaacaa gaaccaggtc aatggaagca
    20821 tgatcgctaa atgggaaatg acgtagaaaa caggacccat cacattacca ctatccgttg
    20881 ataaaccgag ggcaatgctc aagagaattg ggatcaaagt tcctacataa agggaatcgc
    20941 caattcctgc gaatggtccc atcaaagctg ttttgatgcc gctgatgaga tctgcagaat
    21001 cttctttgtt caccgcgctc tgttcttcca tgcccagaac gatgccgggg acgatggatc
    21061 cgatgtacgg ttcggtgttg aagaaggctt catgtcgagc caaaagctct ttttgagctt
    21121 ccttgttccc aggatacaat ttttctttta cttttccgag catgcggatg atggcgggag
    21181 cctccattct ctcaaagttc tgcacagaaa ggttccagaa catccagttc caataagcac
    21241 tgtgcacatc tttctttgat aggagttttt tcttgtcgtc cgtcttttcg gttttggtct
    21301 tcttcaggac tgaaaaatcg gtctgcgtgt agcgcaggtc catatacgcg aaaagcaagc
    21361 cgaacaaaac gacagggatg atctgaatct tgaaaacagc caccaggata aaaccgatca
    21421 aatagtagat gaggtccagt ttctttttca ccacatattg caacaacaga gcgaacccaa
    21481 ccagcggcag tatgctgccc agcagagtca gaattccgcc caaccactcg ggaatcaggc
    21541 taaccatcgt aggaacgaat tgggctccca aatagtttgc aaggaagatc gggataaagc
    21601 ggcaaacgaa gttggtgatc tgccccacaa caggaatgtt catggctttt tccaacttgc
    21661 catccgtaat gtagcgatcc atcttgtgga caaacacaga attgaacgat gccgtcgcat
    21721 agactgccat aacccccatg aaactcagag caatcgataa gcttacagca taagatgcat
    21781 cagctccgct aatgatagcc agcggaattc caatccaggc agcaaagttg acatcagccg
    21841 gcatggaacc accaggagta accaaaccaa tatacattgc ctggatcgcc gcaccgatga
    21901 taatgccctg ttgaacatca cctaaaatca gcccgatgat caatcccgaa accagaggtc
    21961 ttcctatcgt ataccatcct cccagcaagc caataagcca gggagaatag atcgatgaga
    22021 agtagccaaa aattgaaatg agtaatgctt gccaaattgt cattttttac ctcgctttta
    22081 tatcttctga atttgatata accagcttta cttgcacaaa tcttttactt cgttccattc
    22141 aattattttt tgatcaattg tctgattaaa atatacccgc accctccttt cttcgatttt
    22201 attgacagca tcgatttcct cccttgaaag atacgtatac ggagttacct ttattgtttc
    22261 cggtcgatag ctgatagggc caatattcaa ttcttttttt agatttggat gaatttcaaa
    22321 taatttcaac gccaccgtgg gtgaacgcat gaggagcaaa acgttttcac tattttcaat
    22381 aaatggggga agacttgctc tggtttcttc gatcgaatag ataacggact ttgcttgagg
    22441 aggagtagcc aggttaaaaa tattcttcaa aatcggatta gaagccgtaa agtcatcaac
    22501 aataacaatt ttttcaattt taaaatcaac gattaccctt acaatacatt gaccgtggat
    22561 cagtcgatca tcaaccctat ttaacgtgat catacattct ttatccttcc gatatcattc
    22621 ttttagtaat aattcgaatg ctatctttgc cttttgcaac gatatcattc aataaatcgt
    22681 caaaatccag gtactggttt ctcactgaaa gtatttcagc aatcatcggt aaattcacac
    22741 ctgcaatgac cgccgcatcg atatcaacca atgtcattgc agccgcattg gttggcgagg
    22801 cgccaaacag atccgtaaag atcacgtttt tatggctccc catatttctt atttgctcga
    22861 taatcttctt gctcaaatcc ataatgtcgt ccccctcata aagcccgatg gtttcaaaac
    22921 actcttgcgg acccatcaac atttcaatcg cttgcttaaa gctatcggca agcgcagaat
    22981 gcgtacacac tacaatccct actgtttcgt tttgattcgc tgatgacata ttatttttaa
    23041 taccctagct acctaaaaat cacatcaaaa atatatttcg ttccatggta gatggaatcg
    23101 gcatacgcaa caggggtcat gttcgcatca aaatccacgc cattcatgcg cattcccacc
    23161 cctttatctt tctcgaaaag tatctttgct tcattccctt caaccatgac cgcatttatt
    23221 ttttcctgtt tcaagacaac ctcagcgttg tatttggttt ttaaaatttc atagagggaa
    23281 ttttcttcaa aagaatacat ctctaatccg gggaatcgct tttcaggaag gcagacattt
    23341 tcaatggcaa taggttccag atcagccaat cttactcggc tcaagtagta gacattttcc
    23401 gttggcagta tcttcaattt ttcagcgaca gatatcagcg gtgttttgat ctcgaaagat
    23461 aagactttac tggttggcgt atacccgagc tgcttcatat ggtaagaaaa accaagcacc
    23521 tccagggatt tccgatacac ccaatgggac acaaaagtgc ctctgccctg aatgcggtag
    23581 aggtatccat cgttcaccag ttcatcgata gcttttctga cggtcatccg gctggtattg
    23641 aactgattgc agaattcctg ttcagaggga atgatattgc ctttctggta gataccgttt
    23701 tcaatgtatt cttttatata agcgatgatc tgctgatata tgggtatagt ttctttcatt
    23761 acacctccgg tttcaagaaa cttcacaaga aagttacaat atgaacattg ccttgtttat
    23821 atatacaagt tgtatatatg tcttgtatat attataggac aaagaacgtg aaagtcaata
    23881 gggtcgttac agtacttttg ttagctctac tggcagttgg gaagtgcgtt gaggaagcaa
    23941 ctacatctgc cctcccacat acattgtaga agggaggaag taaatgctgt atgaaaaagg
    24001 tgtgcgggga acgcgcaccc agccgttttg gagcaggata tctttaaaat agggtaaaaa
    24061 tcctgagatt tccaacgcaa tatggagagg ttccccagtc gctacgctcc ttctgaatga
    24121 cgttgggggc aggaaatgga aaagcgatca ctagctggtg ctttcaataa ataaaataag
    24181 ggattcctcg gtcgcgatgc ttccgcggaa caactgaggt aaggtggatt gagaagcgta
    24241 ccgcaattga acacaaagaa gaacttgtct tctgtcaatc actataatag tttcccatca
    24301 gttctctgtc aaggctaagt agaaagatcc taaaggtgag ccaaatagaa agatcctaag
//
LOCUS       DAOSDS010000027        23994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_940_length_23994_cov_4.558002, whole genome shotgun sequence.
ACCESSION   DAOSDS010000027 DAOSDS010000000
VERSION     DAOSDS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 23994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23994
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_940_length_23994_cov_4.558002"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="PLL88_04875"
     CDS             <1..1199
                     /locus_tag="PLL88_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOJ00931.1"
                     /translation="LLPLEPNDSYQSESYKPSLTYEMLQSAGDAYPARVKRVYLQLPE
                     NFSERIQTLAEAITDRLDSDFEKTLAITEYLRTQMAYSTELGELPRNLDPVFWFLFEQ
                     KQGYCNYFASAEVLLLRSIGIPARLGVGYAQGVEIERGKVFEIRSRDSHAWSEVYFPE
                     IGWVIFEPTSAQPAIHFPYESVAAQEEVEGRTLQETQPNEIGLRENAATGSNMTAWFD
                     AIEERLAGQERLYEFGYAKPGKAWVTPAILTALAGGSVLFLFLGRVRYQGKRIPLQEY
                     MVTVFEKRGRTSPQWLKKWAAYRNATYLQRTFPQFDLMLAVFGVKNLPQRTPLEKATL
                     LAHFVPEAQKEIEHIVQAYQQEAFGKQAHGKGNLPVYFRHLRKAALGAWFTARAKRLK
                     KRWVKK"
     gene            complement(1153..2205)
                     /locus_tag="PLL88_04880"
     CDS             complement(1153..2205)
                     /locus_tag="PLL88_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOJ00932.1"
                     /translation="MPVQENINTIICGSLHQETIINALDQVSLNHPGGSLLYAASGYA
                     LCDRRIGLVGKTHAQFLTDFHQDLEEITTALEGITLSVHLPNDIRYYRIRSREKWETS
                     HFKRHFYELGQEVPKFLLALERPSSNTFSMSPPCNQPLTSSDFPPQYVGAKALLLTPM
                     DFLSHFSCIPHLRRMGIQKVLIRSSPSYMIPAKLCALPKLLNGIDFFFTTEQEVKTLF
                     GARFDRYQTMLEILCRFGAAHVVIKNSQDGYLLLHGDDQRATRVPDHFVFTVDPIGEY
                     DCFCGVFTACLATTSRSIEVCAAYASAAASICREGSGVRYILNTYPDILRLRAEMVAH
                     QIISSPIASLTASREL"
     gene            complement(2224..2430)
                     /locus_tag="PLL88_04885"
     CDS             complement(2224..2430)
                     /locus_tag="PLL88_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00933.1"
                     /translation="MEEKKNTNIIYLIGGVAGALLGVTIAHVLVRSSESKNKPIQLSA
                     QQGLQIGLQTAGFARALLNLLNKS"
     gene            complement(2432..2833)
                     /locus_tag="PLL88_04890"
     CDS             complement(2432..2833)
                     /locus_tag="PLL88_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00934.1"
                     /translation="MPDTISYERVVMNKRAILAQIWIPLVIGIILVGVMAYFLFDSAV
                     SGSTQLSQWSDISLIFLLVPIIGFSLLALIIGIFCVFFTHAAHKQLQVWLETAASYTT
                     NWNHKIRGLCQKGISLASGPSEWLKNSRRKE"
     gene            complement(2839..4884)
                     /locus_tag="PLL88_04895"
     CDS             complement(2839..4884)
                     /locus_tag="PLL88_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00935.1"
                     /translation="MLRKVIDRLSSLTTKKGFVPIILLLFTLLAFAPLIPFLGFYWDA
                     WPYLWQYHVFGPSGYPAFVASDRPYSAFIFQILTYLFGTRAIYYHVFTLLCRWLSGWA
                     FWWILQQVWSRNRTLNIYAAILFILYPGFLQQPIALPYCHHFSHMALFLFSVGAMLAA
                     LRDRKRSWLYTVLALIAQLNIFSLEYFASLEFIRPFLMWFMLRDEIPDTKQRLKKTMR
                     LWAPYLVLLAFFYYWRIFLFSFPTYEPKMLNELSQDFSTGIFNLMLQALKDLLTVTYG
                     AYKNIFLFPKVSEVGYFAAYGYWGILCISLIITSFILIAVEAKEDRGRTQRDPKQGIA
                     VFVTGLLSLLFAGVIFWITKLSVRIEFAWDRLTLAYMFGMALLIASLVFWLLRPKHLR
                     IAFTASLVSLTIAFHFLNAMAFKHDWDTFKDFFWQLSWRVPDLKENTIIMTTLFPLKY
                     YSDNSLTAPLNWMYDPESKDDTLSYMFYFSDVRLKVGRLTALEKDLPVEQWYRSFSFS
                     GNSTDTVVIRYDPSGCLQVLDVRYANAGIIPNLTDLEAAQIPLSNLDRIITSSIDEKH
                     PPIEIVGEEIEHGWCYYFEKADLARQTADWDVVLTLKDEADAAGFSPRNPSEWLPFFE
                     AYLQTEQWDKAVDLIHASLAVDDKYTPGILFTWDRVMSERHLEPPGEILALIASLRD"
     gene            5233..5898
                     /locus_tag="PLL88_04900"
     CDS             5233..5898
                     /locus_tag="PLL88_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOJ00936.1"
                     /translation="MNRRVITGIEQQKKNPYRVNIYLDDQFAFGLYKAVACYLKVGDM
                     LDEKDIQALEEKDTTENAYQKALKLLSYRPRTEHEMRTRLEGYGFLQSTVDAVMAMLF
                     EKGYLNDQQFAEEWIENRTTFRPRGKRLLRLELVKKHVGEEDIQSALAALPEEERLIR
                     ETARKYSARLKDLNEDGFKKRLYGFLVRRGFLYEDIKPVLTELWEEHARLNSEENEVL
                     ENE"
     gene            5891..7411
                     /gene="rny"
                     /locus_tag="PLL88_04905"
     CDS             5891..7411
                     /gene="rny"
                     /locus_tag="PLL88_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOJ00937.1"
                     /translation="MSDTVRIIIEICGAAILAVVLGIVIGKARTKEITQKSEQLIQES
                     KETARETELRARDAALKIKQEAEAEISRRRTELSHEEDRIQNRRTSLDQRFERIEQRE
                     LSLNKRQSAIDKRMNEIETLYQQEIEKLQQLSGITSEQAKQMLLQEVEKESRGDMARI
                     YRQIEAEARAEGEKRAREIVTESIQRVASDQVAQITTSTVSLPNEEMKGRIVGRNGRN
                     IRAFEQAAGVDVIVDDTPEAVTISCFDPTRREIARRSLQRLIDDGRIHPAHIEKILAE
                     EKKEVEKVIEEAGEEAAFDAGVAGLPPQVLTMLGRLKFRTSFGQNQLAHSVETARIAA
                     LIASELGGDVEIAKAAGLLHDIGKAMDHNVEGTHAQIGAEFCKRNRVNDAVVNAIASH
                     HHETEQQTLEAVICEAADAISGARPGARRENLEQYIKRLKALEEMANSYEGVSQAYAI
                     QAGREVRIIVEPEEIDDLEATRLARDIAKNIEETMQYPGQIRVTVIREMRAVDFAK"
     gene            complement(7466..8107)
                     /locus_tag="PLL88_04910"
     CDS             complement(7466..8107)
                     /locus_tag="PLL88_04910"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ00938.1"
                     /translation="MLPSVHIDTFVLGQIQNNTYLISDERNQQAVVIDPAAGISELVA
                     VLNQKRLQLSAIWITHAHFDHIAGVYQLLHTCGSDVPLYLHPDDLPLWRSGAGARDFG
                     FDFDPRVEPNAFFQHGQLIPFAGTNIAVRHTPGHAPGHVLLYWEEQQVAFCGDLIFYH
                     SVGRTDLPQGDETILMRSIQNEVLTLPDLTTLLSGHGPATTVGEERKNNPFLY"
     gene            complement(8115..8867)
                     /locus_tag="PLL88_04915"
     CDS             complement(8115..8867)
                     /locus_tag="PLL88_04915"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ00939.1"
                     /translation="METFLMHTAEPFLFKGDDLGFLLIHGFTGTPKEMRLLGEHLAAQ
                     GHTILGIRLPGHATRIEDMRRMRWQDWAAAVEDGVALLRSCCSKVISVGLSMGGALSM
                     YAAANFDLNAAVALSAPYVLDDKRLPLLRPLSLFKPYLAKGRSDMKDAEQAAVHANYT
                     AYPTKSIIELDQLLKATRAVLPYISIPVLLIHSKADQGVSFRNLERYYKKIGSNQKTK
                     LELEKSGHVVTEDIEREVAFQAISDFAQKVMK"
     gene            complement(8876..9976)
                     /locus_tag="PLL88_04920"
     CDS             complement(8876..9976)
                     /locus_tag="PLL88_04920"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOJ00940.1"
                     /translation="MTRIETLQKFMLEKKIPLVAVTAGPDLRYLTGLDFHVSERPAIL
                     LLAASGQSHLVHPQLESEKVKSCKFPLVSFPYGEAKDTWAEVCAQALNGLSSEKTKIG
                     VISTQMRYLEMELLHKALPELRFTAIDEGMQKMRTQKDISEISNIRKAIAIAEQAFLD
                     TIRTIEAGRTEKEIAATLVLNLFHSGSDPELPFSPIIASGPNSADPHAVPTERVVQAG
                     DLIVIDWGATHNHYISDLTRTLSIGAPLAEFEQITSVVKAANSAAREKAEKHILCSEV
                     DAAARAVISDAGYGDYFTHRTGHGIGMETHEAPYIAVDYKQPLEIGNTFTIEPGIYLP
                     GRGGVRIEDNMVITEQGAETLSSLPRDLFIIE"
     gene            complement(9963..12266)
                     /gene="metE"
                     /locus_tag="PLL88_04925"
     CDS             complement(9963..12266)
                     /gene="metE"
                     /locus_tag="PLL88_04925"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HOJ00941.1"
                     /translation="MVGTSNLGFPRIGFQRELKRGLERYWANAISLQELLEITASIRL
                     QNWQRQHDLGIEVLPSNDFSLYDHVLDTIAMVGAIPQRYRSITAEFDLDVYFAMARGV
                     QKNDVDIPAMEMTKWFNTNYHHIVPEIEAHTTFSFTYHKILDEYREALMAGFQTRPVL
                     LGPLTFLLLSKTSTPDFSPIQKLDELIPVYQLILSELFNAGAEWVQVDEPMLVKDLPQ
                     AIRIAYQSAYQTITASSHRPQILLASYFDELGENFELAFSLPVEGVHLDMVSGWEQWE
                     RLNADAVKSKLLSLGIVDGRNIWWADLERLLSYLEKKSASLQNEILIAPSCSLLHVPL
                     DVGLEAHIDQPVASWLAFAVQKLEELSALAGALNSGRRPHLFSRNRAALESRRDFLSQ
                     SNSKSGTDQMEQQPVQRKNPFSVRKKKQSSHLHLPLFPTTTIGSFPQTAEVRQMRSRS
                     TKAEISQEHYHDFLRQEIARTIQLQEQLGLDVLVHGEFERNDMVQYFAERLHGFVFTE
                     NGWVQSFGSRYVRPPILFGNVERPQPMTVEWSSYAQSLTSKPVKGMLTGPVTILQWSF
                     VRDDQPRSQTCFQIAAAINAEVLDLEKAGIRIIQIDEPALREGLPLKKNHWQEYLDWA
                     VKAFHLACANVADETQIHTHMCYSEFNEIIASIAALDADVISIEAARSSMELLNAFSN
                     FKYPNEIGPGIYDIHSPRVPMVTEMVALLEKATSVIPAEQLWVNPDCGLKTRNWQEVL
                     PALQNMISAAQEMRNKFSKRDHGNDQN"
     gene            complement(12288..12953)
                     /locus_tag="PLL88_04930"
     CDS             complement(12288..12953)
                     /locus_tag="PLL88_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOJ00942.1"
                     /translation="MNPTPTIEDYLGVMYTLDCDGESIIGAKLSELLEVSAPTVTTTL
                     KRMIRDQWITMDEKKEIHLTKKGTAAAVSIIRKHMLTEWMLSRILDVPWSQVHEEAHK
                     IEHSISMDVAEKMQERLDDPKLCPHGNPFPGFEKMTKDWVALSELEQGDRFIIRRIHE
                     FGEEDLELMTFLQKNEIKPAQMGTVKEKLPFNETITLQIARSTVSLGFKTAKMIFVEK
                     IPE"
     gene            13180..13947
                     /gene="map"
                     /locus_tag="PLL88_04935"
     CDS             13180..13947
                     /gene="map"
                     /locus_tag="PLL88_04935"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOJ00943.1"
                     /translation="MIELRTKHEVEALRAAAQITAQTHARLAEAIHVGVRLKDLDEIA
                     EAYIQAQGATTLYKGIRQVPNQRPFPGVICASVNNEICHGLPDGRTLKDGDIVGIDIG
                     LRYKGWCGDMCVTHMVGQVKPEVKKLVQTTYESLQRGIAACVPGNRLGAIGAAIQTYA
                     ESEGFSVVREYGGHGIGRELWEDPFVPHIGPADRGPILRAGMVINVEPMLNMGKPYTK
                     LMKDEWTVVTADGSLSAQFEHTIAITENGPEVLSVLS"
     gene            13971..15155
                     /locus_tag="PLL88_04940"
     CDS             13971..15155
                     /locus_tag="PLL88_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ00944.1"
                     /translation="MNKNIRYVLFATLYMAQGAVMGYFTSLNALYLQTFGLGMDKIGI
                     IGSIAMIPFVLKIFFGLLSDKVSFFRLGHRKPYILIGLLVQMLGLLAVILINPATHFG
                     LFALNAFLLMAGMALYDTCTDGLALDTTAPEDEGKIQGIMVAGRALGMILISGVLGLL
                     VDRISWFAGFLSLAVLTAIPIPLVLQMKEAAKPVDKQFNWSAFREFKKFSIIALGLLG
                     ALYSLIINGTSQLVNPYLEQKFTISYTTAGIISSVLGIGIVVGSLLGGKLVDRIGQRK
                     SVQLSLLVTLLAVGALCLISTPVVAWMLVFIFGVAFGLYETVYFAIAMRETNPSIAAT
                     MFSILMAVANLGTGIGLALSGSLAEGLGYKPTFLILALMNLLALPLLPAIFGGRKSEQ
                     AE"
     gene            15216..16301
                     /locus_tag="PLL88_04945"
     CDS             15216..16301
                     /locus_tag="PLL88_04945"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HOJ00945.1"
                     /translation="MMGANVVIGGFWGDEGKGVISAWEAFRTGSLGVFRGCGGANPEH
                     GMFIDDHYIKVNQLPLGFILQECMIGIGPGTAVDPAKLLHEVKRFHLTPEKVRVDRRC
                     PIITVENIASEKANANMQAIGSTFSGTGIAMAHAALRIASIAEDVKELRPYLDDVPAL
                     VNDLAKRGKVNLEGTQGTFLSRYHGEYPNVTSVDVTTSSVVAGTGLNFRDVEDVIVAV
                     KSLPTREGSGAMGDVEEFTSEEMEELGIVEYSSIVDLKSGEHQIRRKAKGIDYTFLNR
                     VIMLNGANKLALTFAEQYDPQVRGVTRWSELTRKVRELIDKIESMTNVEVTLVNTGKE
                     FDCLASKRDDLPPVTEAMRTRLRAYIE"
     gene            complement(16340..16978)
                     /locus_tag="PLL88_04950"
     CDS             complement(16340..16978)
                     /locus_tag="PLL88_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOJ00946.1"
                     /translation="MESIPADLLGEVIVSQDKIGLIVNRVAKNIFYSRDYIDNLPILL
                     CLREGALPFFNDLKKELDQLHFAYDSAELTAKSYKGAQSSGSVEITAYTGPLLKGRSV
                     IIVEDVIDSSNTIVAIFQYLLAQGVRKQDICTLLFKEKPENSEWRKTGLDGFKKYPEI
                     RYVGLFIPDQFIVGYGLDYRNFGRDCKDIRVLTPQGQAWVNAQHEDCDPLHS"
     gene            complement(17105..18583)
                     /locus_tag="PLL88_04955"
     CDS             complement(17105..18583)
                     /locus_tag="PLL88_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOJ00947.1"
                     /translation="MKLQRISLLRWIAIALLVLSAVILVLELVRFSRLRAGFPAGTVI
                     GGIPVGGLDQQQAADRVIEAYNFPIEVHHGGEIFYLKPSSLGFKVDIDTMIASADQQR
                     VNAPFWSSFWNYLWNRNPAAQETPLAYSIDEKQLKMFLYDEIAARYNNDPEQSQPVAG
                     STQFKIGTPGKVLDVDASLPFVEAALSSATNRVVNLVVSEIDPPRPSIDNLEVLLKQL
                     IDESAFDGLTEVYVLDLETGKEINFAYENGIDYNPGIAFTAASTMKIPIMVTVFQYLP
                     EPITETAATMMQLMIDKSSNDSSDDLMETYLSPTLGPLIVTDTMQQLGLESTFLAGHF
                     YIGAPLLQRFITPANSRTDYTTDPDPFNQTTTAEMGMLLEDIYQCAQTGGGTFAALFP
                     NEISQNECQTMVTYLTNNKIAVLLEAGLPDGTRIGHKHGWTSDATTGLMYSLIDTGIV
                     FSPAGDYVICVAMYHPVQLIFDKANLLTANISKAVYNYFNIE"
     gene            18660..20342
                     /locus_tag="PLL88_04960"
     CDS             18660..20342
                     /locus_tag="PLL88_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOJ00948.1"
                     /translation="MNISKEKLLKALKGGIIFRDIPDNFIQWVIERSHILQFQEGDLI
                     YEKGAAAERFFFVISGSAVLYVQEGEQQYLINGCQAGNYFGFEALSKRKMRLTNAKAE
                     SPLLTLAIPLRIVQKIAEQFPAFQVQFTLQLQSLEFLIKKPMNWLQNEEIVHYVNREH
                     PLILITRVLKPIILSLAALTLAGVLFNANVITPAMLLWIGSCLGAFGIGWAVWNAFDW
                     MNDYYVVTNQRVVFLQKIALIYDSRKETPLSAILSITKQIQLIGRFYQFGDVILRTFT
                     GLVKFRNVAYVEAVSAIVEEQWLNHKEKIAEENQEDPEIYLQKRLLGQNGASQNNQAK
                     QAEKPAEDILSSGYYPDLFSRILKLRIVEEDTIIYRTHWFVFIRKTILPFLGMVLSVI
                     FLLAPAYGWFGLVAEGLGTYQSILIATGISMALWWIYAAVDWRNDYFLITPEQIVDVN
                     RKPLGMEERRSAPVRNIQTIEYKRQSIFGLLFNFGTVFIRVGDVEFTFDYVPHPSYVQ
                     QEIYTRFQRIREGEKKENADLNNERLASWMDAYHRVIRTQMPTPEGEEGEQD"
     gene            20402..20917
                     /gene="def"
                     /locus_tag="PLL88_04965"
     CDS             20402..20917
                     /gene="def"
                     /locus_tag="PLL88_04965"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOJ00949.1"
                     /translation="MNGREVIKLPHPNLRKKAREVRDFGDDLQELVDDMIITMREEDG
                     VGLAAPQINQSLRLIVVEYPEDDSQEESEKKLYIMANPVIVKRSEECELGIEGCLSVP
                     DIVGEVERAVQVEVHGLNRFGKKTKIRAKGWLARVFQHEIDHLDGVLFVDRATRLFKT
                     SDEEAQQAEQV"
     gene            20932..22155
                     /locus_tag="PLL88_04970"
     CDS             20932..22155
                     /locus_tag="PLL88_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOJ00950.1"
                     /translation="MFLKHISLTNFRAFQRLEVDVPERVTVMVGNNAQGKTSFLEAIN
                     FFSTLTSVQAGKDRELINFMALGDEIPVARLVMNFSRGGSEHNMEARLILGNNGNKGS
                     RLRKEVLLDGIKRPLQQALGNFNSVIFLPQMTDIVDGAPDERRRYLDMAIAQVYPGYA
                     RELSEYNQAISQRNALLKTLAERGGDSAQLDFWDEHIAEKGSFIMRTRMQMLDEFEDR
                     VSEVHLALSEGISPLAINYLPGFNFRNGEREQETFAFPVGKDSHACLLSQEELQNTFL
                     KKLQSVRKDEIQRGMTTLGPHRDELRFFANQIDSGTYGSRGQIRTVLMALKIGEMHWL
                     HEKTGEWPVLLLDETLAELDLQHRNALLHALSNCEQAILTATDAGMFNPEFLKTCTIW
                     KIDAGRIDFEQSSFL"
     gene            22245..>23994
                     /locus_tag="PLL88_04975"
     CDS             22245..>23994
                     /locus_tag="PLL88_04975"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HOJ00951.1"
                     /translation="MDVITDRSIRVFISSTFRDMQGERNYLVKFIFPQLRKICEQRGV
                     AWSEVDLRWGITDEQSAEEQVLPICLEEIKRCRPYFIGILGERYGWIPEKVNSELIER
                     EPWLAEHLDHSVTELEILHGVLNNPEMADRALFYFRDPAYVQDLPTERQEGFLEDLAV
                     HEMEEYGREEAQKRALDRKTKLTALKERIRKSGLSLKEGFQDPQQLGTWVLEDMTAII
                     DRIYPEGSQPTPLKLEGQEHEAFAVNRAKVYIGGEKYFKKIDEHVAGTNLPLVVLGES
                     GFGKSALLANWGLKHRMAHPDKLVLMHFIGASAASGDWSAMLRRILSELQERFNIQGE
                     IPTDVTELRETLPDWLHIAAQKGRVLLILDALNQLEDKQGAQELTWLPMEFPENVQVI
                     LSTLPGKTLDSLNKREYPVLFIEPLTRQEREKFVSVFLAQYSKQLNSQQVGKIVEDPK
                     LSNPLGLRILLDELRQFGIHERLNEIIQHYLNANSVNEMLELVFERCEKDFETERPGL
                     VRDTLCNIWAARQGLSEGELLNLLGNDGQPLAQRVWSPLHLALEESLLEHSGLLNFSH
                     AYIRQAVEKHYLATESE"
BASE COUNT         6253 a         5421 c         6511 g         5809 t
ORIGIN      
        1 ctcttttacc gcttgaacca aacgattctt atcaatctga atcctataag ccttccttga
       61 cctatgaaat gctgcaatca gccggcgatg cctatcctgc ccgggtgaag cgggtgtact
      121 tgcaattgcc agaaaatttt tcagaacgga tccaaaccct ggcagaagcg atcaccgata
      181 ggctggattc ggatttcgag aaaacgctgg cgatcaccga gtatttacga actcagatgg
      241 cttattccac cgaattgggg gaactccccc gcaatctcga cccggtgttc tggttcttgt
      301 ttgaacaaaa acagggttac tgcaattact tcgcttctgc tgaagtgctg ctgttgcgtt
      361 ctatcggaat tcccgcacgg ctgggggtag ggtatgccca gggggtggaa attgaacggg
      421 gcaaagtgtt tgaaatccgt agcagagaca gccatgcctg gtcagaagtg tactttccag
      481 agatcggatg ggtaatcttt gaacctacca gtgcccagcc cgccattcat tttccatacg
      541 agagcgtcgc tgctcaggag gaagtggaag gcagaacgct gcaagagacc cagcccaatg
      601 agataggact gagagaaaat gctgcaaccg gtagcaatat gaccgcctgg tttgatgcga
      661 tcgaagaaag actggcgggg caggaaagat tatatgaatt cggatacgca aaaccgggca
      721 aagcctgggt cacccccgcg atcctgacag ccctggcagg aggaagtgtg ctttttctat
      781 tcctggggag ggtccgttat caaggaaagc ggataccgtt gcaggaatat atggtgacgg
      841 ttttcgaaaa gcgcggaaga acatctcccc agtggttgaa gaaatgggcg gcatatcgca
      901 acgccacgta tctccagcgc acctttccgc aatttgacct catgcttgct gtgtttggtg
      961 tgaaaaacct gcctcaacga accccactgg aaaaagcgac cttgctggcg catttcgtgc
     1021 cagaagctca aaaagagatc gagcacatcg ttcaagcgta tcagcaggaa gcgtttggaa
     1081 aacaagcgca tggtaaaggg aacctccctg tttatttccg ccatcttcgt aaggcagcgc
     1141 taggggcgtg gttcacagct cgcgcgaagc ggttaaagaa gcgatgggtg aagaaataat
     1201 ttggtgcgcg accatttcag ctcggaggcg caaaatatcc ggataggtgt tgagaatgta
     1261 acgaacgccg ctcccctcac gacagataga agctgcagca ctggcgtagg cagcacacac
     1321 ttcgatggaa cgtgaggtgg tggcaagaca agcagtgaag acgccacaaa agcaatcata
     1381 ttcaccaatg gggtccacgg tgaaaacaaa atgatcaggc actcgtgtag cccgctggtc
     1441 atcaccatga agaaggagat agccatcttg ggagttttta atgaccacgt gtgctgcccc
     1501 aaatctgcag agtatttcca gcatagtctg gtaacgatca aaacgtgcgc cgaacagagt
     1561 tttcacttcc tgttcggtgg tgaagaaaaa gtcaatacca ttcaacagtt tgggaagagc
     1621 gcacaatttc gcaggaatca tatacgaagg ggatgaacgt ataaggacct tctggattcc
     1681 cattctgcga agatggggga tacaggaaaa atgtgacaga aaatccatgg gggttagcag
     1741 cagcgctttc gcacccacgt attgtggggg aaagtcggaa gaggtcagag gctgattgca
     1801 aggcggtgac attgaaaatg tattggagga aggtctttcc aacgcaagga ggaatttggg
     1861 aacctcttgt cccaattcgt agaaatggcg tttgaaatgg gaggtttccc atttctctcg
     1921 acttctgatg cggtaatagc gtatgtcgtt ggggagatgg acggagaggg tgatgccttc
     1981 cagggctgtg gtgatctctt ctaaatcctg atgaaaatca gtcagaaatt gcgcatgggt
     2041 tttccccaca agaccaattc ttcggtcgca tagcgcatag ccgctggcag catataagag
     2101 acttccacct gggtgattca gactaacctg atccaacgcg ttgatgatgg tttcctggtg
     2161 gagagacccg caaatgatgg tgttgatatt ttcttgcacg ggcatgttgt gaaaacaatc
     2221 tttttacgat ttattcaaca aattcagcag agcacgggcg aaaccggctg tctggagacc
     2281 gatctgtagc ccctgttgag cagaaagctg gatgggtttg tttttggatt cggaggatct
     2341 gaccagcacg tgagcgatgg ttacacccag caacgctccc gccacaccgc cgatcaggta
     2401 aatgatattg gtattctttt tctcttccat attattcttt tctccgtgag ttttttagcc
     2461 attccgaagg tcctgaagcg agcgatattc ctttctgaca caacccccgt atcttatgat
     2521 tccagttggt ggtgtacgat gccgctgttt ccaaccaaac ctggagttgc ttatgcgctg
     2581 catgcgtaaa gaaaacgcag aagatgccaa tgatgagagc gaggagtgaa aatccaatga
     2641 tggggacgag gaggaagatc agggaaatat ctgaccattg ggaaagctgt gtgctgccac
     2701 tgacagcaga gtcaaagaga aaatatgcca tcacccctac caaaatgata ccaatgacca
     2761 aaggaatcca gatttgtgcc aagattgctc ttttattcat cacgaccctt tcataggata
     2821 ttgtatccgg catttctcct aatcgcgtaa cgaagcgatc agtgccagga tctccccagg
     2881 tggttcgagg tgtcgttcgc tcatgactct atcccaggtg aaaaggatgc ccggtgtata
     2941 tttgtcatcc actgccaggc tggcgtggat cagatcaaca gctttatccc attgttcggt
     3001 ttgaagatag gcttcaaaga aaggcaacca ttcactgggg ttgcgcggag aaaaccctgc
     3061 cgcatctgct tcgtccttga gcgtgagcac cacatcccaa tcagcggtct gacgagccag
     3121 atcagccttt tcaaaataat aacaccaccc atgctcgatt tcttccccca ctatctcgat
     3181 gggagggtgt ttctcatcaa ttgacgaggt gataatgcgg tcaaggttgg agagtggaat
     3241 ctgtgctgct tccagatcgg taaggttggg gatgatgccc gcattggcgt agcggacatc
     3301 cagaacttgc aaacagcccg acggatcgta acggatgacg accgtatcgg tgctgttgcc
     3361 gctgaaagag aatgagcgat accactgctc tacgggcagg tccttttcga gtgcggtcaa
     3421 tcgcccaact ttcaaacgga cgtcggaaaa gtagaacata taggacaatg tgtcgtcctt
     3481 tgattctgga tcatacatcc aattcagggg agcggttagc gagttgtcac tgtagtattt
     3541 caaaggaaag agcgtggtca taatgatggt attttctttg aggtcaggaa cacgccaact
     3601 caactgccag aagaaatcct tgaaagtgtc ccaatcgtgt ttgaaagcca tggcattgag
     3661 aaaatggaag gcgatggtca aacttaccag agaagcggtg aacgcaatgc gcagatgttt
     3721 ggggcgtagc aaccagaaca ccaggctggc aatcagcaac gccataccaa acatatatgc
     3781 cagggtcaga cgatcccagg cgaattcaat gcgcaccgag agcttggtaa tccagaagat
     3841 gacaccggca aaaaggagag aaagcagacc tgttacaaac accgcaatcc cctgctttgg
     3901 atcacgttgt gttcgccctc tatcctcttt cgcttcaacg gcgatcagaa tgaaggaggt
     3961 gatgatgagc gagatgcaga gaattcccca gtagccgtat gcggcgaagt atcccacctc
     4021 cgaaacctta ggaaagagaa agatgttctt gtacgccccg taggtgaccg tcagtaaatc
     4081 tttcaaagcc tgcagcatga gattgaaaat gcccgttgag aaatcctgcg atagttcatt
     4141 caacatcttg ggttcatacg ttgggaaaga gaacaggaaa attctccaat aatagaagaa
     4201 agccaacaaa accagatagg gagcccacaa tcgcattgtt tttttcaagc gttgttttgt
     4261 atctgggatt tcatcgcgca gcatgaacca catcaagaat gggcgaatga attcaagtga
     4321 agcaaaatat tccaatgaga agatattcaa ttgggctatc agcgccaaga cggtgtacag
     4381 ccagcttctc tttcgatcgc gcagcgcagc cagcatggca cctacagaaa acaggaacaa
     4441 tgccatgtgg ctgaagtggt ggcagtaagg aagggcaatg ggttgttgga gaaaaccggg
     4501 gtagagaatg aaaaggatcg ccgcgtagat gttcaatgta cgattgcgcg accatacctg
     4561 ttgtaggatc caccaaaaag cccatccaga tagccaacgg cagagcagcg tgaagacgtg
     4621 ataatagatg gcgcgagttc cgaagagata ggtcaagatc tggaaaatga aagcggaata
     4681 gggacggtca gaagctacaa aagcagggta tccggaagga ccgaagacgt gatattgcca
     4741 gaggtatggc caggcatccc aatagaagcc gaggaaggga atcaggggag caaatgccag
     4801 aagggtgaaa agtaacagga ttatagggac aaagcccttt tttgttgtaa gtgacgataa
     4861 gcggtcgatg actttcctga gcatactctt tattctactt gtttccgatg atatttcgcc
     4921 atgcaatatc gaatttcctg tgcccaacct taaaaactcc gcttttcgca aaataaaaaa
     4981 tattgtataa tgattgggta tttaaaagat attttcggga cgtacaagtc ctatttatca
     5041 ggtttaaggc aatgtttgga aattggaact actcctaagg gagtatatgg gaaaacagaa
     5101 cagtacaatt ttgggatatt aagtttgatc aagcgtgttt tgtttaagca gacagtatag
     5161 attttattaa acccattctc aacggaatca gttgccattt tctaccttaa cctggtgtgt
     5221 taaggtattt ttatgaatag acgagtaatt actgggatcg aacaacaaaa gaagaaccca
     5281 tatcgagtga atatctatct ggatgaccaa ttcgcctttg gtctctataa ggcggtggct
     5341 tgctatttaa aagtcgggga catgctggat gaaaaagata tccaggcact tgaagaaaaa
     5401 gacacaaccg aaaacgctta tcaaaaagct ttgaaactct tgagctaccg cccacgcact
     5461 gaacatgaga tgcgcacgcg gttggaagga tatggtttct tacaatctac ggtggatgcg
     5521 gttatggcta tgctttttga aaaggggtat ctcaatgatc aacagttcgc agaagaatgg
     5581 attgaaaacc gcaccacctt tcgtccaaga gggaaacgtc ttttacgtct ggagttggta
     5641 aagaaacatg tgggggagga agatatccaa tctgcactgg cggcgcttcc agaagaagag
     5701 cggctaattc gcgaaactgc cagaaaatat tctgcgcgat tgaaagattt gaacgaggac
     5761 ggttttaaga agagattgta tggtttttta gttcgacgcg gatttctcta tgaggatatc
     5821 aaaccggtat taactgaatt atgggaggaa catgcacgct taaattctga agaaaacgag
     5881 gtattggaaa atgagtgaca cagtacgaat aatcatcgaa atatgtggcg cggcgatcct
     5941 ggctgttgtt ttgggaattg tcattggaaa agccagaacg aaagaaatca ctcaaaagag
     6001 cgaacaattg atccaggaaa gcaaagaaac ggcgcgcgag actgaactgc gagcgcgtga
     6061 tgcggcatta aagataaagc aggaagcgga ggcggagatc agccgacggc ggactgaact
     6121 ttctcatgaa gaagatcgca tccagaacag gcgaaccagc ctggaccagc gctttgaacg
     6181 cattgagcag cgagaattga gcctcaacaa acgacagagc gccattgaca agcgcatgaa
     6241 cgaaatagaa acgctttacc agcaagaaat cgaaaaatta cagcaacttt caggcattac
     6301 cagcgaacag gcgaaacaaa tgctgttgca ggaagtggaa aaggaaagcc gcggggatat
     6361 ggcgcgcatc taccgccaaa tcgaagcaga agcgcgcgcc gaaggtgaaa aacgcgcgcg
     6421 ggagatcgtc acagaatcta tccagcgcgt ggcttctgat caggtggctc agatcaccac
     6481 ttccacggtg tcgttaccca atgaggaaat gaaggggcgc atcgtggggc gtaacgggcg
     6541 taatattcgc gcgttcgaac aggcagccgg ggtggatgtg attgtggatg acacgcccga
     6601 agcggtgacc atctcttgtt tcgatccgac ccgccgcgag atcgcgcgca ggtcactgca
     6661 acgcctgatc gatgatgggc gtatccatcc cgctcacatc gaaaagatcc tggcagaaga
     6721 aaagaaagag gtagaaaagg ttattgagga agccggtgaa gaggcagctt tcgacgcagg
     6781 tgtcgccggt ctgccgcccc aggtactcac tatgctggga aggttgaaat tccgcacatc
     6841 cttcgggcaa aaccagcttg ctcattctgt cgaaaccgcc cgcattgcag cgttgatcgc
     6901 atcagaattg gggggggatg ttgagattgc caaggcagcc ggtttactgc acgatatcgg
     6961 caaggcgatg gatcacaacg tggaaggaac gcatgcgcag attggcgctg aattctgcaa
     7021 acggaaccgg gtgaacgatg cagtggtgaa cgccattgcc agccatcacc atgaaaccga
     7081 acagcaaact ctggaggctg tcatctgcga agcagcagat gccatctcgg gcgcacgacc
     7141 tggcgcgcgc agagaaaacc tggagcagta tatcaagcgc ctgaaagcct tggaagaaat
     7201 ggcaaattcc tacgaaggcg tcagccaggc gtatgccatc caggcagggc gcgaagtgcg
     7261 catcatcgtt gagccggaag aaatcgatga tctggaagcg accagattgg cgcgggatat
     7321 tgccaagaac attgaagaaa ccatgcagta tcccgggcag atccgcgtga ccgtgatccg
     7381 cgaaatgcgc gcggtggatt tcgcaaaata ggcatgaaaa agcgcaggca ggtgaccaac
     7441 ttcgcggtta cctgtctgtt gttttttaat ataggaaagg attgtttttc cgttcttccc
     7501 cgactgttgt ggcaggaccg tgcccgctta acagggtggt gaggtcgggc agcgtcagca
     7561 cttcgttttg gatgctgcgc atcaatatcg tttcatctcc ctgcggcaga tcggttcgac
     7621 caacgctgtg gtaaaaaatc agatcaccgc aaaacgctac ctgctgctct tcccaataaa
     7681 gaagcacatg accgggagcg tgcccggggg tgtggcgcac tgcaatattc gttccggcaa
     7741 agggaatgag ctgaccatgc tggaagaaag cgtttggttc aaccctggga tcgaaatcaa
     7801 aaccgaaatc tcgtgcgcct gcccctgacc tccagagcgg cagatcatcg gggtggaggt
     7861 ataagggaac gtctgatccg caggtgtgaa gcaactgata tacaccggca atatggtcga
     7921 aatgagcgtg ggtgatccag atggctgaaa gctgaaggcg cttttgattc aacacagcta
     7981 ccagctcgct tatccccgca gccgggtcga tgacaaccgc ttgctggttg cgctcatcgc
     8041 tgatgagata ggtattgttt tggatttgac ccaagacgaa tgtgtcgata tgaacagaag
     8101 gaagcatgat cctgttattt catcactttc tgtgcaaaat cggagatggc ttggaatgcc
     8161 acttcgcgtt cgatgtcttc cgttaccaca tgcccgcttt tctcgagctc cagttttgtc
     8221 ttctggtttg agccgatttt tttgtagtag cgctcgaggt tgcggaagga taccccctgg
     8281 tcggcttttg aatgaatgag cagcaccgga atggatatat agggaagtac tgcgcgcgtg
     8341 gctttcagca gttgatccag ttcgatgatg gatttggttg gataagcggt atagttggcg
     8401 tgcacggctg cctgttcggc atctttcatg tcacttcgtc ccttggcaag gtagggctta
     8461 aagaggctca gtgggcgcag taaaggtaag cgtttgtcat caagaacgta gggggcagaa
     8521 agagccactg cagcgttcag gtcgaagttg gctgcggcat acatggataa cgcaccaccc
     8581 atcgatagcc ccacgctgat cactttggag caacaggagc gcaagagcgc taccccatct
     8641 tccaccgcgg cagcccaatc ttgccagcgc attcggcgca tgtcctcgat gcgggtggca
     8701 tgaccgggca ggcgaattcc caaaatggta tgtccttgag cggctaagtg ctctcccagc
     8761 aggcgcattt cttttggggt gcctgtaaaa ccatggatca gcaagaaccc aaggtcatca
     8821 cccttgaaaa ggaatggttc agcggtatgc attaaaaaag tttccatcac tcctcctatt
     8881 cgatgatgaa aagatcacgc ggtaacgaag ataatgtctc agctccttgt tcggtgatga
     8941 ccatattatc ctcaatcctc accccgccgc gaccgggcaa atagatgccg ggctcgatgg
     9001 taaaggtgtt cccaatctct agtggttgtt tgtaatcgac agcaatgtaa ggggcttcat
     9061 gcgtttccat tccgattcca tgaccggtgc ggtgagtgaa ataatcgcca taaccggcat
     9121 cgctaatcac cgcccgcgcg gcggcatcca cctcgctgca caggatatgt ttttccgctt
     9181 tttctcgcgc ggcgctgttg gcagctttta cgacgctggt gatctgttca aattcagcca
     9241 gcggcgcgcc aatggaaagg gtgcgggtga gatcggagat gtagtgattg tgcgttgcgc
     9301 cccaatcaat tacgatcaga tctcctgcct gtaccacccg ttcggttggg acagcgtgcg
     9361 gatcagccga attgggacca gaagcgatga tgggactgaa gggcagttcg gggtcagaac
     9421 ctgagtggaa aagattgaga acgagtgtgg cagcgatctc cttttctgtg cgcccagcct
     9481 caatggtgcg gatggtatcc agaaatgcct gttccgcgat cgcaattgct ttccgaatgt
     9541 tcgagatctc gctgatatct ttttgggtgc gcatcttctg catgccctca tcaatggctg
     9601 taaagcgaag ctcaggcagg gctttatgca gcagctccat ctccagatag cgcatttgcg
     9661 tggagatgac gccgattttc gttttttccg aggaaagtcc attcaatgcc tgggcgcaga
     9721 cttctgccca ggtatctttt gcttctccat aggggaaaga aaccagcgga aatttacacg
     9781 attttacttt ttcgctttcc aattgtgggt ggaccaggtg ggattgcccg ctggctgcca
     9841 gcagcaagat tgccggtctt tcagaaacat gaaagtcaag cccggtgagg taccgcaggt
     9901 cgggtccggc tgttactgcc accagcggga tctttttttc cagcatgaat ttttgcaagg
     9961 tttcaattct ggtcatttcc atggtccctt ttgctgaatt tgtttcgcat ctcttgagct
    10021 gcggatatca tgttttgcag tgcgggcagc acttcttgcc agttccgcgt tttcagaccg
    10081 cagtcagggt ttacccaaag ctgctcggct gggatcacgc tggtggcttt ttccagcagt
    10141 gccaccattt ctgtcaccat cggaacgcgc ggcgaatgaa tatcatagat gcccggtccg
    10201 atttcattcg gatacttgaa attggaaaag gcgttcaaca gttccatgga tgaacgggcg
    10261 gcttcgatgg agatgacatc tgcgtcaaga gcggctatcg aggcgatgat ctcgttgaac
    10321 tcggaataac acatatgggt gtggatctgc gtctcatcag ccacattcgc acacgccaga
    10381 tgaaaggctt tcacagccca atcgaggtat tcctgccagt ggtttttttt aagtggcaat
    10441 ccctcgcgca gagcaggttc gtcgatctgg atgatgcgaa tgccagcctt ttccaaatcc
    10501 aacacttcgg cattgatggc agcagcgatc tggaagcaag tttgcgacct gggctgatca
    10561 tcgcgcacga aagaccattg cagaatggtg actggtccgg tgagcatccc tttcaccggt
    10621 tttgaggtga gcgactgcgc atagctgctc cattccactg tcatgggttg cgggcgctcc
    10681 acgttgccaa acaggatggg agggcgtaca tagcgcgaac cgaaactctg cacccaacca
    10741 ttttccgtga agacaaaccc atgcaaccgt tcggcgaaat actgcaccat atcgttgcgc
    10801 tcaaattcac catgcacaag cacatccaac ccgagctgtt cctgtagctg gatggtcctt
    10861 gctatttcct gccgcaaaaa atcgtgataa tgctcctgag atatctctgc cttggtagaa
    10921 cgagagcgca tttggcgaac ctcggcggtc tgagggaagg atccgatggt ggtggtggga
    10981 aagagaggaa gatgcagatg ggagctttgt ttcttcttac gcacggagaa aggatttttt
    11041 ctctgcacgg gttgttgctc catctggtct gtacctgatt ttgaattgct ctgggatagg
    11101 aaatcccttc ggctttccag ggctgcccgg ttgcgggaga agaggtgagg gcgcctcccg
    11161 ctattgagag cgccagccaa tgcgctgagc tcttccagct tctggacggc gaatgccagc
    11221 caacttgcca ccggctgatc aatatgcgct tccagaccaa catccagggg aacgtgcaac
    11281 aaagaacagg agggggcgat caagatttca ttctgcaagg acgccgattt tttttccagg
    11341 taagacaaca aacgttcaag atcagcccac cagatattcc gcccatccac gattcccaaa
    11401 gaaagcagct tacttttgac ggcgtcagca ttcaggcgct cccattgttc ccaacctgaa
    11461 accatgtcca ggtgaacgcc ttcaacgggg agagagaagg caagctcaaa attctctccc
    11521 aactcatcaa agtatgaagc gagcaagatc tgggggcgat gggagcttgc cgtgatcgtt
    11581 tgatatgcgc tctggtaggc aattcggatc gcttgaggga ggtccttcac cagcatcggt
    11641 tcgtcgacct gcacccattc ggcgccagcg ttgaacaatt cggacaggat gagctgatat
    11701 acagggatga gctcatccag tttttggatg ggcgaaaaat caggggtgga ggtcttgctt
    11761 aacaacagaa aagtaagcgg tccgaggagc acaggacggg tttgaaaacc agccataagc
    11821 gcttcgcgat attcatcgag tattttgtgg taggtgaacg aaaaagtggt gtgcgcttca
    11881 atttcaggga cgatgtggtg gtagttggta ttgaaccact tggtcatttc catggcgggg
    11941 atgtccacat catttttctg aactccgcgc gccatggcaa aatagacgtc cagatcaaac
    12001 tcagcagtga tcgaacgata gcgttgcgga attgcgccga ccattgcgat ggtgtccaac
    12061 acgtgatcat acaacgagaa atcgttggag ggcagaacct cgatgccaag gtcatgttgg
    12121 cgctgccagt tctgcaggcg gatgcttgcg gtgatctcga gcaattcttg caatgagatt
    12181 gcatttgccc aatagcgttc aagtcccctt ttcaattcgc gctgaaaccc gatgcgtgga
    12241 aaacccaggt ttgaagtacc taccatctct atgctcctcc taccggctca ttctggaatc
    12301 ttctcaacga agatcatctt tgctgttttg aaccctagcg aaacagtgga acgggcaatc
    12361 tgaagggtga tcgtttcgtt gaagggaagc ttttctttaa ccgttcccat ctgcgcaggt
    12421 ttgatctcat tcttttgcag aaatgtcatg agctccaaat cttcttcacc aaactcatgg
    12481 atgcggcgga tgatgaaacg atcgccctgt tccagctctg acagcgcgac ccaatccttg
    12541 gtcatctttt cgaagccggg aaatggattg ccatgagggc acaatttggg atcatcgagg
    12601 cgttcctgca tcttttctgc cacatccata gaaattgaat gttcgatctt gtgcgcctct
    12661 tcgtgcacct gcgaccaggg aacatccaat atcctggaaa gcatccattc ggtcaacatg
    12721 tgcttgcgga tgatactgac tgccgctgcc gtgccttttt tggtaagatg gatctctttt
    12781 ttctcatcca tggtgatcca ctgatcgcgg atcattcttt taagcgttgt cgtaacggta
    12841 ggggcggaaa cctcgagcag ttcagaaagt tttgctccga taatgctttc tccgtcacaa
    12901 tccagcgtat acatcacgcc aaggtaatct tcaatggttg gggtgggatt catttcagtc
    12961 aacgcacacc tcacttagta tctgctaatc aaaattatat acacattcga ttatagccca
    13021 agaacgggta cccctcatca aagaaagata agaaatttag ccttttcttg cagagaacga
    13081 gcacgactat catttttcta ttaaaatgcg tagaatagaa agaattgaaa ggttgggttt
    13141 tgatctgcaa ccgaaaccga cctcaaattg gaggtttatt tgatcgaact aagaacaaag
    13201 cacgaggtcg aagcgttgcg ggcggcagcc cagatcaccg cacaaacaca cgcacgcttg
    13261 gctgaagcga tccatgtagg cgtgcggtta aaagacctgg atgagatcgc agaggcatac
    13321 atccaagcgc agggcgcgac cacattatat aaagggatcc gtcaggtgcc caaccaacgt
    13381 ccttttccag gggtaatctg cgcatccgtt aataatgaga tctgtcatgg tcttcccgat
    13441 ggaagaacgt taaaggatgg cgatatcgtt gggatcgaca ttggattgcg ctataaaggg
    13501 tggtgcggtg atatgtgcgt gacacatatg gtgggtcagg tgaaaccaga agtgaaaaaa
    13561 ctggtccaga ccacttatga aagcctgcaa cgtggaatcg cggcttgtgt tccgggcaac
    13621 cgcctggggg cgattggagc agcgattcag acctatgcgg aaagcgaagg cttttcggta
    13681 gtccgtgaat atggtggtca tgggatcgga agagaattgt gggaggatcc atttgtgccc
    13741 catatcggtc cggcagatcg gggaccgatc ttgagagccg gcatggtgat caacgttgaa
    13801 cccatgctga atatggggaa accgtatacc aaactaatga aagatgaatg gacggttgtt
    13861 actgccgatg gcagcttatc ggcacagttc gagcacacca tcgccattac cgagaacgga
    13921 ccagaagtat tatctgtctt atcgtaaatc gtcctaagga ggaatgcacc atgaacaaga
    13981 atataaggta tgttctgttc gccacactgt atatggcaca gggagccgtg atgggatatt
    14041 tcacctctct gaatgccctg tatttgcaaa catttggatt aggaatggat aagattggga
    14101 tcatcggttc tatcgccatg atcccttttg ttttaaaaat cttctttgga ttgttaagcg
    14161 ataaggtgag cttcttccgc ctggggcatc gcaagccata tatcctgatt gggttattgg
    14221 tgcagatgct tgggctgttg gcagtgatct tgatcaaccc ggctacccat ttcggtctgt
    14281 tcgccttgaa tgctttcctc ctgatggcgg ggatggcatt gtatgatacc tgcaccgatg
    14341 gattggcgct ggatactacc gctcccgaag atgagggaaa gattcagggc atcatggtgg
    14401 cggggcgcgc cctgggtatg attctgattt cgggagtgct gggattgctg gttgacagga
    14461 tctcctggtt tgccggcttt ttatcccttg ccgtcctgac cgctattcct atcccgctgg
    14521 tgctgcagat gaaagaagcg gcgaagccgg ttgacaaaca gttcaactgg agcgctttca
    14581 gggaattcaa gaaattttcc atcatcgcgc tgggtttgtt gggtgcactg tattcgctga
    14641 tcatcaacgg taccagccaa ctggttaacc cttacctgga acagaaattt accatcagct
    14701 atacaaccgc agggatcatc tcgtcggtgc tggggatcgg gattgtggtt ggcagtctgc
    14761 tgggaggcaa attggtggat cgcattgggc agagaaaatc ggtgcagcta tcgctgttgg
    14821 tgacgttgct ggctgtgggt gccctgtgtt tgatcagcac gccggttgtc gcctggatgc
    14881 tggtatttat ctttggggtg gcgtttggtt tgtacgagac ggtatacttc gcgatcgcta
    14941 tgcgtgaaac caaccccagc atcgctgcca ccatgttttc gatcttgatg gcagtggcga
    15001 accttggaac cggtattggg ttggcattaa gcggttctct ggctgaaggg ctggggtaca
    15061 aaccgacctt cttgattctg gcattgatga acctgctggc gcttcctctg ttgcctgcca
    15121 tttttggagg gaggaaatcg gagcaggcag agtagggtaa gcctttaata ttagggtcta
    15181 gttaagcata aaaatttctt accagaggat gatggatgat gggagcgaat gttgtcattg
    15241 gagggttttg gggagatgaa ggaaagggag tgatcagtgc gtgggaggcg tttcgcaccg
    15301 ggtcgctcgg agtatttcgc ggatgtggag gggcgaatcc ggaacatggt atgttcatcg
    15361 atgatcacta tataaaggtg aaccagttgc cattgggatt cattttgcag gaatgcatga
    15421 tcgggatcgg accgggcacg gcagtggatc cggcgaagct cttgcacgag gtcaaacgat
    15481 ttcatctcac acctgagaag gtaagggtcg atcggcgctg tccgatcatc accgtggaaa
    15541 atattgccag tgaaaaggcg aatgcgaaca tgcaagccat tggcagcacc ttcagcggga
    15601 cggggattgc tatggcacat gccgcgctgc ggatagcctc cattgccgaa gatgtgaaag
    15661 aactacgacc ttatttggat gatgtgcctg ctctggtaaa tgatctggca aagagaggca
    15721 aggtcaacct ggagggtacg cagggcacct ttctttccag atatcatggg gagtatccca
    15781 atgtcacctc cgttgatgtg actacctctt cggtggtagc tggcaccggt ttaaactttc
    15841 gcgatgtaga agatgtgatc gtagccgtca agagcctgcc cacgcgtgaa ggcagtgggg
    15901 cgatggggga tgtggaggaa tttacctctg aggaaatgga agagttaggc atcgtcgaat
    15961 acagttccat cgtggacctg aagagcgggg aacatcagat ccgcagaaaa gccaaaggga
    16021 tcgattacac ttttctcaac cgcgtcatca tgctcaacgg cgccaataaa ctggcattga
    16081 cttttgccga gcagtatgat ccgcaggtga ggggagtaac caggtggtcg gaattgacca
    16141 gaaaagtgcg cgagttgatc gataagatcg agagtatgac caatgtggag gtcaccctgg
    16201 ttaacaccgg caaagaattt gattgcctgg cgagcaaacg ggatgatctg ccccccgtca
    16261 cagaagcgat gagaaccagg ctgcgtgcct atatcgagtg atgaaagaac ctctgttgtg
    16321 ttgcccagca gaggttcttt catgaatgca gcgggtcgca atcctcatgc tgcgcattga
    16381 cccaggcttg cccctgggga gtgaggacac ggatatcctt gcagtcgcga ccaaaattgc
    16441 ggtaatcaag cccgtacccc acgatgaact gatcagggat gaacaacccc acataacgga
    16501 tttcgggata tttcttgaaa ccatccaaac cggtcttgcg ccattcggaa ttttcgggtt
    16561 tttccttgaa aagaagcgtg cagatatctt gcttgcgcac gccctgcgcg agcagatatt
    16621 gaaagatagc aacgatggtg ttggaagaat cgatcacgtc ttccacaatg atcaccgatc
    16681 tgcctttcaa aagaggtcct gtgtaggcgg tgatctcaac gctaccgctg ctctgcgcac
    16741 ctttataact cttggcagtt aactctgcag aatcgtatgc gaagtgtaac tgatccagtt
    16801 cttttttcag gtcattgaag aaaggcagcg cgccctctct caggcagagc aagatgggga
    16861 ggttgtcgat ataatcgcgg ctgtagaaaa tatttttagc gacacggttg acgatcagac
    16921 cgattttgtc ttgactgacg atgacctctc cgagcaaatc tgcgggaatc gactccataa
    16981 tgtgttcctg ttgtcagaaa atctgcttga atcatacccg ctttctaaaa atctgttctt
    17041 aaaaaaattc catcctcgcc aaagaatgga attttttatg atgggaagcg agccaaagcc
    17101 cgacctattc gatgttgaaa tagttataaa ctgctttgga aatgttggcg gtgagaaggt
    17161 tggctttatc gaagatcagt tgaaccgggt ggtacatggc aacgcagatc acgtaatccc
    17221 cggcggggct gaagacgatg ccggtatcga tcaacgaata catcagaccg gtggttgcgt
    17281 cggaggtcca accatgctta tgaccaatgc gggtgccatc gggcaaaccg gcttccagga
    17341 gaacagcaat cttgttattg gtcaggtagg tgaccatagt ctggcattcg ttctggctga
    17401 tctcgttggg aaacagggcg gcgaacgtcc ctccgccggt ctgggcgcat tggtagatat
    17461 cttccaaaag cattcccatt tcggcagtgg tggtttggtt aaaggggtcg ggatcggtgg
    17521 tgtaatcggt gcgagaattg gctggcgtga tgaagcgttg cagcaaaggg gcgccgatat
    17581 aaaaatgacc agccaggaac gtactttcca acccgagctg ctgcatcgta tcggtgacga
    17641 tcagcggacc cagggtaggg ctgaggtaag tttccatgag atcgtccgat gagtcgttgc
    17701 tagatttgtc gatcatcagt tgcatcatgg ttgcggcagt ttcggtgatc ggctcgggca
    17761 agtattggaa gactgtgacc atgatgggta ttttcatggt gctggcagcg gtgaaagcga
    17821 tgccgggatt gtaatcgata ccgttctcat aagcgaaatt gatctctttg ccagtttcca
    17881 aatccaagac ataaacttcg gtcaacccgt caaacgctga ctcatcgatc aattgtttca
    17941 gtaatacttc cagattgtct atagaagggc ggggtggatc gatctctgag accaccagat
    18001 tcacgacccg gttggtggca gatgaaagcg ctgcctccac gaaaggtagt gaagcatcca
    18061 catcgagcac tttgccgggt gtgccgatct tgaactgggt gcttcctgca accggctgtg
    18121 attgttctgg atcgttatta tagcgagcag cgatttcatc gtagaggaac atcttgagct
    18181 gtttttcatc aatggaatac gccagaggcg tttcctgcgc tgcagggttg cggttccaca
    18241 ggtaattcca gaaagaactc cagaaagggg cgttcacgcg ctgctggtcg gcagatgcga
    18301 tcatggtgtc tatatcgacc ttgaaaccca gcgaagaggg tttcagataa aaaatttcac
    18361 cgccatgatg aacttcaatg gggaagttat aagcctcgat gacgcgatcg gctgcttgtt
    18421 gttggtcgag cccacctacc ggaatgccac cgatgacggt gccagccgga aagccagctc
    18481 gcagccggct gaatcgcaca agttccagca cgaggatgac cgcagagagg accagcaagg
    18541 cgatcgcgat ccaacgcaac agagaaattc tctgtaattt cataagcatc tcctgattct
    18601 gaccttccat tttaccagaa gggatttgaa acatgaaata ttggatatac ttaaatcgta
    18661 tgaatatttc aaaagaaaag ctactcaaag cgttgaaggg tgggattatc tttcgggaca
    18721 tacccgataa tttcatccaa tgggtcatcg aacgctccca tatcctccaa ttccaggaag
    18781 gcgacctgat ctatgaaaag ggagccgctg ccgaacgctt ctttttcgtc atttccggca
    18841 gcgctgtact gtatgtacag gagggggagc aacaatatct gataaacggc tgccaggctg
    18901 ggaattattt tggtttcgag gcgctttcaa agcggaagat gcgtttgacc aatgccaaag
    18961 cggaatcccc gcttctgacg ctggcgatac cgttgcggat cgttcagaag attgcggaac
    19021 aatttcccgc ctttcaagtc cagttcactt tacaattgca gagcctggaa ttcttgatca
    19081 aaaaacccat gaattggttg caaaacgagg agatcgtaca ttatgtcaat cgcgaacatc
    19141 ctcttatcct gatcacgcgc gtgttgaaac ccatcatcct ctcactggcg gcgttgacgc
    19201 ttgccggcgt attgttcaat gccaatgtga tcacccctgc catgctgctg tggatcggat
    19261 cgtgccttgg ggcgttcggg attggatggg cggtatggaa tgcgtttgat tggatgaatg
    19321 attattatgt ggtgaccaac cagcgcgtgg ttttcctgca aaagatcgcg ctgatctacg
    19381 atagccgcaa ggaaacgcca ttgagcgcta ttctttccat aaccaaacag atccagttga
    19441 tcggcaggtt ttaccaattt ggcgatgtga ttttgcgcac tttcaccggc ttggtgaaat
    19501 tccggaatgt ggcttatgtt gaggcggttt cagctattgt ggaagaacaa tggctgaacc
    19561 ataaggaaaa gatcgcagaa gaaaaccagg aagaccccga gatatacctg caaaaacgcc
    19621 ttttgggtca aaacggagcc tcacaaaaca accaggcaaa acaagctgaa aaaccagcag
    19681 aagacatcct gtcgtctggt tattatccag atcttttttc gcgcattctc aaactgcgca
    19741 tcgtcgaaga agataccatc atctaccgta ctcattggtt cgtgttcatc cgcaagacca
    19801 ttctgccctt tctggggatg gtcttgagtg tgatcttctt gcttgcccca gcttatggat
    19861 ggttcggatt ggtggcggaa ggattgggga cctaccaatc catcctcatt gcaacaggta
    19921 tcagcatggc gttatggtgg atctatgctg cggtagattg gcgtaacgat tatttcctga
    19981 tcacacccga gcagattgtg gatgtgaacc ggaaaccgct tggcatggaa gaacgccgtt
    20041 ctgcgcctgt tcgcaacatc caaaccattg aatacaagcg ccagagcatc ttcgggttgc
    20101 ttttcaactt tggcacggta ttcatccgcg ttggggatgt ggaattcaca tttgattacg
    20161 tgccgcaccc ctcctatgta caacaggaga tctatacgcg ttttcaacga atcagagaag
    20221 gtgaaaagaa ggaaaatgct gatttgaata acgagcgctt agccagttgg atggatgcct
    20281 atcaccgggt tatacgcacg caaatgccaa cgccagaagg ggaagaaggg gaacaggatt
    20341 gagcgtgtta taatttggat aatttttata ataaaaataa tattatttgg atgtgcaagc
    20401 gatgaatggt agagaagtga tcaaattacc gcatcctaac ctgcgcaaga aggcgcgcga
    20461 agtgcgcgat ttcggggatg atcttcaaga gttggtggat gatatgatca tcaccatgcg
    20521 ggaagaggat ggcgtggggt tggctgcgcc gcagattaat caatcactgc gcctgatcgt
    20581 ggtcgagtac ccggaggatg acagccaaga agaatcggaa aagaaactgt atatcatggc
    20641 aaaccctgtg atcgtgaaaa gatccgaaga atgcgaatta gggatcgaag gttgcctttc
    20701 tgtaccggat attgtggggg aggtggaaag ggctgtgcag gtggaagtgc acggattgaa
    20761 ccgatttgga aagaagacaa agattcgcgc caagggctgg ctggcgcgcg tctttcaaca
    20821 tgagatcgat catctggatg gcgtactttt cgttgaccgc gccacaaggt tattcaagac
    20881 cagcgacgaa gaagcacagc aagcagaaca ggtgtaaaca acccgattcg catgttcctg
    20941 aagcatattt ctctgaccaa ttttcgagcg ttccagcgac tggaagtgga tgttccagag
    21001 cgtgtaacag tgatggtggg caacaacgca caagggaaaa ccagcttctt ggaagcgatc
    21061 aacttctttt cgacgctcac ttccgttcag gctgggaaag acagggagct gatcaatttc
    21121 atggcgctgg gggatgaaat tccggtggcg cgactggtga tgaacttcag ccgtgggggg
    21181 tcagagcaca acatggaggc gcgtttgatc ctgggcaaca atggtaacaa gggcagccgg
    21241 ttgcgcaaag aagtgctgct ggatggcatc aaacgccctc tccaacaggc gttgggaaat
    21301 ttcaattcgg tgatcttctt gccgcaaatg accgatatcg tggatggggc gccggatgag
    21361 aggcggcgct atctggatat ggccatcgcg caggtgtatc cgggttacgc gcgcgaactc
    21421 tcggaatata accaggctat cagccagcgc aacgcattgt tgaaaacgct ggctgaacga
    21481 ggaggggata gcgcacagct tgatttttgg gatgaacaca ttgcggaaaa gggcagcttc
    21541 atcatgcgca cgcgcatgca aatgctggat gaatttgaag atcgggtttc agaagtgcac
    21601 ctggcgcttt ctgagggaat atcgccactg gcgatcaatt atctgccggg gttcaatttc
    21661 agaaatggcg agcgcgagca agaaaccttc gcttttcctg tgggcaaaga ttcccacgcc
    21721 tgtctgctca gccaggaaga gttgcagaac acttttctca aaaaattgca atcggtccgt
    21781 aaagatgaga tccagcgcgg aatgaccacc ctgggaccgc accgcgatga actgcgtttt
    21841 ttcgctaacc agatcgattc aggcacctac ggttcgcgtg ggcagatccg cacggtgctc
    21901 atggcgctca aaattgggga aatgcattgg ctgcacgaaa agaccggaga atggccggtg
    21961 ctgctgctgg atgaaacgct ggcagaattg gacctgcagc atcgcaatgc gctgctacat
    22021 gctctatcca attgcgagca ggctattctt acggctactg atgcaggtat gtttaatccg
    22081 gagttcctta aaacctgtac gatttggaaa attgatgcgg ggcgaattga ttttgaacaa
    22141 agttcttttt tgtgaaacaa gtaaaatcaa atagaatata aataaagagc acctttttca
    22201 tcgaaggaac aaaaaaatca acttaatgat caagagaggg ttctatggat gttatcactg
    22261 atcgttctat tcgagtgttt atttcctcaa cttttcgtga tatgcaggga gaacgtaatt
    22321 atctggtcaa atttatattt ccccaattaa gaaagatttg cgaacagcgt ggtgttgcct
    22381 ggagtgaggt ggacctgcgc tgggggatca ccgatgaaca atcggcagaa gaacaggtgc
    22441 tgccgatctg ccttgaggag atcaagcgct gtcgcccata ttttattggt atcctcggtg
    22501 aacgttacgg atggatcccg gaaaaggtca attcagagtt gattgaacgt gaaccgtggt
    22561 tggcagaaca tctggaccat tccgttactg agttggaaat tctgcatgga gtgttaaaca
    22621 atccagagat ggcagatagg gcgctctttt attttcgtga tcctgcatat gtccaggatc
    22681 ttccaacgga acgccaggag ggttttttgg aagatctggc ggttcatgag atggaagaat
    22741 atgggcgcga agaagcgcag aaaagggcgc tagaccgcaa aaccaaactg accgcactaa
    22801 aagagcgcat ccgtaagagt ggactgtcat taaaggaagg gttccaggat cctcagcagc
    22861 ttggaacatg ggtgctggag gatatgactg ccattatcga tcggatttat cccgaaggtt
    22921 cacaacctac accgttgaag ctcgaaggac aggaacatga ggctttcgct gtgaaccggg
    22981 caaaagtgta cattggcggt gaaaaatatt ttaaaaagat cgatgagcat gtggcaggca
    23041 ccaatttgcc tctggtcgtt ttgggtgaat ctggatttgg aaagtctgcg cttttagcga
    23101 attggggatt gaagcatcgt atggcacacc ctgataagtt ggtgttaatg cacttcatcg
    23161 gagccagcgc ggcttcaggc gattggtctg ccatgctgcg caggatcttg agtgaactgc
    23221 aggaacgatt taatatccaa ggggaaatac ccacagatgt aactgaatta cgggaaacat
    23281 tgcctgattg gttgcatata gctgctcaaa aagggcgggt actgctcatt ctggatgcgc
    23341 taaaccaatt ggaagataaa caaggagcgc aagaattgac ctggcttcca atggaatttc
    23401 cggaaaatgt gcaggtgatc ctttcgacat tgccgggaaa gaccctggat tcattgaata
    23461 aacgagaata tcctgttctc tttattgagc cccttacccg gcaggaacgg gaaaaattcg
    23521 tgagcgtgtt tttggcacag tattctaaac aattgaactc acagcaagtg ggaaaaatcg
    23581 tagaagatcc aaaactctcc aatccattgg gattgcgcat tttgctcgat gaattgcgtc
    23641 aatttgggat acatgaaaga ttgaatgaga tcatccaaca ctacttgaat gcgaattctg
    23701 taaatgaaat gttggaattg gtatttgaac gttgcgaaaa agattttgaa accgaacgtc
    23761 cgggtttggt gcgcgataca ctgtgcaata tctgggcagc caggcaggga ttatcggaag
    23821 gggaattgtt gaatttgctg gggaatgatg ggcaaccatt agcccaacgc gtctggtctc
    23881 cgctccatct tgccctcgaa gaatctttgt tggagcacag cggtttgctt aatttttctc
    23941 atgcgtatat ccgccaggcg gttgaaaagc attatcttgc aacagaatca gaac
//
LOCUS       DAOSDS010000028        23851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_949_length_23851_cov_5.331568, whole genome shotgun sequence.
ACCESSION   DAOSDS010000028 DAOSDS010000000
VERSION     DAOSDS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 23851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23851
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_949_length_23851_cov_5.331568"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      114..201
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            330..2579
                     /locus_tag="PLL88_04980"
     CDS             330..2579
                     /locus_tag="PLL88_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ00952.1"
                     /translation="MNSKTKKPVGAQAMRLGERLVGAGYITREQCKAALAYQKIHGDL
                     FGHTLVEMGILTAQQLGDFFAGGGMALSGERLVHAGIITREQLVWALNYQEENGGRLG
                     NAIVALGFASRETIDEFFKNTAVADYRKLGDMLVVRGFLTAQQLEQALLFQRNSGGQL
                     GEILISMNFVAPEDLYRTLATQKQLGRIGKRFDFSASKKLPYHVALKHNAIIINNRKD
                     AYILAVHDELNDKATAEIQSFLDKPVEQVLAGMNEIEGLWEMVYQYEQTEDSVYKLYT
                     DQPGNSALLTLSTGQKVTGICLLGVLVLCLLFNYVNTLLVLNVVLQCLYAVFTVLKVI
                     ILIRGLKRHGQLRYTPEQLDAIDERDLPIYTILVPIYKEATVVENLMQRLDALDYPKH
                     KLDIRILLEEDDSETLKAFQKLGVPSHYTLIVVPSTEPHTKPKACNYGLIRARGEITV
                     IYDAEDMPEADQLKKVFLAFRELPESYVCIQSKLNYFNNDQNILTRWFTQEYSTWFDI
                     LLTGVMSIDMPIPLGGTSNHFKTRFLKEIGAWDPFNVTEDADLGIRLYKMNYHTAMLD
                     SYTWEEANSNVKNWIRQRSRWIKGYMQTWLVHMRKPAKLYRELGLKGFIGYQAMVLGT
                     PLLPILNPFFWVMLILWYVFKPPFIGALFPGALYYIAAVQFYLGNFIFVYSNILGTYT
                     VIRGSELTGKMHIRYSVLPSGILLPVYWVLMSVAGYKALVQLILKPSYWEKTHHGLTE
                     QTVLNTTST"
     gene            2615..4852
                     /locus_tag="PLL88_04985"
     CDS             2615..4852
                     /locus_tag="PLL88_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HOJ00953.1"
                     /translation="MKNIKKRICIILALLCMFGGTALTQAPSFVREQEATDVPAQEQP
                     SAEEQTVGTKLFLFREDRTLTMPKSTVSYWFVVPQGISVGQSAVLTLHISYSATLIEA
                     LSSVTVFINGTPVGTRRIVEQAGDEFIWTVEFDSSLIRMTGYNELKITTVHRSLEGDC
                     ADVDNPSNWVVVHSDSFLSLEESAAVDPLLSSYFALYYDTFAEPFTIAGDYVLSDTSD
                     ADIISAMLGLSNASGMYYSDKEYVRLNVYDRDGFSVDNKNKIFIESQEAPGEWSEDLL
                     PLPQQAPVQDGAYVSIAGFTEEYPFYKLFVSGSGRSGLKKATAFLSNRLLVEQAESGD
                     VLLNSDIPVDNVQSSVALNEDGVYSFADYGYADITMAGAFHQSAYLSFAQPGGVSGGE
                     GSYLELRFRHSAVLLSDRSVLNVYVNNVAVSSVKLSPGNTETGSLIVELSASALTQPV
                     INVTIETYHHLGQEVDCIKDYYDVAWTVIDAENSRIVFEKSENALRLSLKNFPYFSSG
                     NTSTVSVYVPGSAGKDALEAVAALAIRAGQNTRMAFEWEIVPSASGLSSVAGDIVIIA
                     EKSDAALPEEIAEKLAITSSGTGYVVRDATLPVIPETLAGKTIIQVIRSPWNFYSSIY
                     VIIYDGAEGLANLERFLGERTELNQLDGQLCVVGDETGVQTFTVSPASDGEDSVPVDR
                     LDHLIAQAEEFTGLPAWLIFSLLAVVAVLIVIVIVMAIKTSKRDEYQDAAEEHRKKQD
                     FFKDE"
     gene            4942..6525
                     /locus_tag="PLL88_04990"
     CDS             4942..6525
                     /locus_tag="PLL88_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00954.1"
                     /translation="MNTVQKKKSGYWGWTWVMFFVVLAAEIVGAYILLYKKGYILTDA
                     IARTANAYYVLYIDPPKLASIGFVWNPLPSFLQIPILLFKDLWPPLATHGVAGGIVTA
                     FFAASNAMILFRTFKHYSISTVLSVILVLLFAFNPFMFYYGCNGMSEMPFFTTIIACV
                     VGLCRWLDERDNHKLLVMMAFSLALGFLCRYEAFAITLGLGISLLIVVFGMKDPSSPF
                     VPKPLRMKAEYAMATGLVLFLPIVYSVVVWIILNWSIMGDPLYFLTSPYSNAAFTSYT
                     LSSDFITAMSSPVKALLFSLGRMLPFLPLFIVITIGRILSKRLFKPDYCVLVVMVGCI
                     SAFHFLMLLSGSSFGWLRFSSFSLPICAAWLPYELSRLKGTVKYLSVAAAVVAFLASA
                     FIVVSYFGSLTRAPEEYWAFVQTDVADATVQRAAAERINEAYSDDVMMMDDSAMSALV
                     VNLDHPERLIISTSDQFNDALEAPWRYGVRYIVVADPFGVGSRDVFNMIYPSLYEEGA
                     DWCVEREDVGGFKVFEVLY"
     gene            6677..7810
                     /gene="proB"
                     /locus_tag="PLL88_04995"
     CDS             6677..7810
                     /gene="proB"
                     /locus_tag="PLL88_04995"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOJ00955.1"
                     /translation="MQYRRIVVKIGTSTLTGGTRAIRHAHIVDLAKQIRSIREEKCQV
                     ILVSSGAIAAGREAMSEASLGKHMPAKQMLAAVGQPRLMAIYEQVFAMYDVRVGQVLL
                     TRTDITNRKSYINARGTMEALLNNTILPIVNENDTIATDEIGIGDNDNLSAMVATMLE
                     ADLLVLLTDTDGIYEGDPTRENAKLIREVRSVEIPQTVWKAVGGSTSGLGTGGMLTKV
                     QAAEIARRGGTTVAVVRGAEGILTRIVGGEKPGTWIYPTMDALENRKRYLLTSAVKGC
                     GVLIDSGAQKALHKGGSLLPVGVTGVRGEFQRGDVIYVFAFDGKPCALGRCAYSSDEI
                     LCIKGLHTEQIEPRLGYIYTDEMIHHDDLVLLQNHGAENENGA"
     gene            7794..9059
                     /locus_tag="PLL88_05000"
     CDS             7794..9059
                     /locus_tag="PLL88_05000"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOJ00956.1"
                     /translation="MKTVLELCRQAREVSFMLPQCAAQQRTQALLALADMLIEGSDAV
                     MRENKIDIQEACKQKVSNASLERLVLSRERIEEMAEGVRAVAGLPDPLGQIFDERDLP
                     NGLHIRKQRVPLGVLAVIYESRPNVTIDVAALTIKSGNAVILRGGREVVRTNAALVDI
                     THQALKRSMIAETSVQFLDDPERRLVDELLTMHAYVDMLIPRGGADLHTYCRENSHIP
                     VITGGIGICHLFVDESADLQKAIEVIVNAKVQRPTVCNALDTLLIHEKIAPVFLPVLV
                     ERMQVEGVVLHLPAELIERYAFTQNDLLVPAKEGDYDREWLSLHMNIDVVPDIGSAIS
                     FIHQHSTAHSDGILTEDQGNAALFLGKVDSAVVYVNASTRFTDGAQLGLGAEVAISTQ
                     RLHARGPMGLQELMTYKWVVQGDYHVRDK"
     gene            complement(9162..9332)
                     /locus_tag="PLL88_05005"
     CDS             complement(9162..9332)
                     /locus_tag="PLL88_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00957.1"
                     /translation="MKLSAPTKLVFWLATILAVLGIIGNFVALPVIGGFTFWLLAAGF
                     VLLWLGNVLKGF"
     gene            complement(9454..9606)
                     /locus_tag="PLL88_05010"
     CDS             complement(9454..9606)
                     /locus_tag="PLL88_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00958.1"
                     /translation="MMQSIILKVNGKEVPLTQFPAEIIIQTILGMLKALKGVEEIKEV
                     EIKITN"
     gene            complement(9614..10063)
                     /locus_tag="PLL88_05015"
     CDS             complement(9614..10063)
                     /locus_tag="PLL88_05015"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HOJ00959.1"
                     /translation="MKDIQPTPWILGFYGRSGIGKTYLVKKIVAQLVKEGFQVAVVKI
                     SDKAISIDTEGKDTFLYGQAGAGTVVFSSAIETAFLIKEPLSSRAVVDLLGQNKAYEY
                     IFIEGALEAWIPKVRLGDMPLRENTVLTYAGDYDTLFSQIKNKEIMK"
     gene            complement(10155..10901)
                     /locus_tag="PLL88_05020"
     CDS             complement(10155..10901)
                     /locus_tag="PLL88_05020"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ00960.1"
                     /translation="MSTLTHEYIQAVVSLLQKVDREEEGAIDQAARLMADAIQNGKRI
                     FGFGCTHSCLPIQEVVYRAGGLMLINPIYAPGIAHLDVHPTTMTSGIEKLSGYAEVIL
                     DNQPIQQGDVLIIISVSGRNAVPIEMAKIARERGITVIGITSRKYSEGVTSRHPSGKK
                     MHDFADVVIDNKAEKGDALLSADGLEPKFASASGITSSAVMHCLSAATIEELLARGIT
                     PPVFVAANVDGGAEHNAKLLKEYRDRIFYL"
     gene            complement(10917..12065)
                     /gene="nagA"
                     /locus_tag="PLL88_05025"
     CDS             complement(10917..12065)
                     /gene="nagA"
                     /locus_tag="PLL88_05025"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HOJ00961.1"
                     /translation="MIQKIIHANICTPDEVLPDHSIEIQQGRIKDIYPTQLSEKPSDA
                     ILDARRDWVIPGLIDIHTHGGVGADTMDASHDALNAMSKFLAAHGVTSFLATTVTNSQ
                     ESINRALLAIEQSQAELEGARLLGAHVEGPYINIAFKGAQNPAYFRPPLKSEYDPWLD
                     NEWVKLITIAPELPGMDEFIRACVERGIELAIGHSSASYEQVIHAADLGVRQATHLFN
                     GMQGLHHREPGTVGGVLTDERIFAQIIADGVHLHPAIVKLVLACKGVNRTILISDAMR
                     ATGLSDGKYDLGGQEVTVIDGTARIANGSLAGSTLTLDAAVRNVMQFTGADFPHVLPA
                     ATRVPAEAMHFARKNGYIRVGYDADLAIFDAGYQVVATIVGGNLVYTK"
     gene            complement(12127..13443)
                     /locus_tag="PLL88_05030"
     CDS             complement(12127..13443)
                     /locus_tag="PLL88_05030"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HOJ00962.1"
                     /translation="MAVFDAILSLITLPAIVLGFIALIGLLLQKKSAGDVFKGTLKTI
                     LGQLIISIGITALISALSPIQVFFEKGIPAGGFETFVTYDEAVVGAVQSANLANIGAE
                     IAWTMLFGYVVHILVSRFTKYRYIYLTGHMIWVHAGAFAILFHSFGLSTWAVVALASI
                     VDGFYMTLMPALAQPVMRKITGSDEIAFGHGQTLLNMLGAWAGKLVGKPEDSSENIKI
                     KDSFNFLRDMAVSISIIMLLVAFIGAGLALNQIGTAAFQEQISGGQNWIVYTLLIALN
                     FTAGVLVLLQGVRMLIAEITPAFKGIAEKLIPGAKPALDCPVIYPYAPNALIFGLISG
                     TIGQVVGMVLLALIGWPVPLPSMIAAFFASGAGAIFGNATGGRKGAWLGGFLWGFVGW
                     LLISFAYKFQVFGDLTSMGAVSLGFTVPDAIVPGIIIWGIAKLFGI"
     gene            complement(13522..13797)
                     /locus_tag="PLL88_05035"
     CDS             complement(13522..13797)
                     /locus_tag="PLL88_05035"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ00963.1"
                     /translation="MVIATLCGMGFGTSMMLKLFIDEILKAEGIKAETSPWDLGTFKG
                     NKADIVVAPVDMEPHLRDYPAKIVLIKNLVDKAELKEKILTAIRELS"
     gene            complement(13805..14266)
                     /locus_tag="PLL88_05040"
     CDS             complement(13805..14266)
                     /locus_tag="PLL88_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ00964.1"
                     /translation="MQLERLLIKSAIRAKLSLETREEVIQKAGDMLVEAGAIKSEYIQ
                     AMKKGIDELGPYCVIAPGIALLHARPEDGVERACLSLLTLRNPVKFGHSTNDPVDVVF
                     ALGAKDKNDHIEALAELAGFLAKEDFLQVLRNSEDSHTLQEKIFEYIKTRK"
     gene            complement(14279..15316)
                     /locus_tag="PLL88_05045"
     CDS             complement(14279..15316)
                     /locus_tag="PLL88_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ00965.1"
                     /translation="MQEDRGKFTQTEILSQPETWKETLRYLQSDWVGKLPHVQDYDQL
                     VITGCGSTYYLSIWAARFLHCTTGVPAMALPSSELWYAGDQWLKPFKKILFIAISRSG
                     QTTETLQALELFLKKHGQSTLAITCYKDSSLAKMSPLAITTTAGMEQSIAQTRSFSNM
                     LWPVILLGEKNISDDLITSIHRSAQKFLSENEPTAREIGRDLDIERFFFLGNGPLFGL
                     AQEIMLKMKEMSLSYSEAFHFLEFRHGPMSMVNHQSLIVGFTSQNTISFELPVFYDMQ
                     KLGGRTLAIGKGTGKPQGIDYLFDFGEKVSSPWVYPFYLPLIQLLAYERSMAKGLNPD
                     KPENLSAVVEL"
     gene            complement(15342..16109)
                     /locus_tag="PLL88_05050"
     CDS             complement(15342..16109)
                     /locus_tag="PLL88_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ00966.1"
                     /translation="MLKEERQQVIIQELGRNGSVAVSELAERFQVSRMTIRRDLFEIE
                     KSNQLKRTHGGAVLPSKSPNYSEPPTIKRMELLKDEKIRIAKAVAALIDEKEVIFLGS
                     GTTTLYVARELYERDDITVLSNAITIINELSTNGKMPVIGIGGFLRREEQSMIGHFAE
                     RVIEDLRVEKVIVGMRGVHPRFGLTSEDPQELMTDRKILGISDNVIIVADHTKIGYVS
                     TSRTAPITAAKTVITTTDAQDDLVRAIREQGVEVMQV"
     gene            16304..17290
                     /locus_tag="PLL88_05055"
                     /pseudo
     CDS             16304..17290
                     /locus_tag="PLL88_05055"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
     gene            17290..18609
                     /locus_tag="PLL88_05060"
     CDS             17290..18609
                     /locus_tag="PLL88_05060"
                     /inference="COORDINATES: protein motif:HMM:NF019625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HOJ00967.1"
                     /translation="MCDMHKNGFSSGEPLQKIIRNHKQGQPVGIYSICSAHREVLCSA
                     MQSQKSRDDLLLIESTCNQVNQEGGYSGMTPERFVVYVQELAREVDFPTGRILLGGDH
                     LGPNPWRREPASLAMGKAGKMVQAYVASGYLKIHLDASMPCGDEAALSDETVAERSAQ
                     LCLLAERAASESGLATLPLYVIGTEVPSPGGMAANQNEVCSVTRVDFAERTVETFQRV
                     FHRHGLEEAFQRIIALVISPGVEFNDKQIFAYDRSRTAGLREFIETIPHMVYEAHSTD
                     YQTPQSLKQMVQDHFAILKVGPALTFAFREAVFRLAAMEEELLGSDKESRSHIVDTLL
                     ETMQSDPFHWEKYYTGSESAIRFALKYSFSDRVRYYWGYPAVQKAYQKLITNLHRRTL
                     PLSLISQYFPHLFPSIRDDTVKADPAALILANVAQVLKHYQNACGSI"
     gene            complement(18619..19221)
                     /locus_tag="PLL88_05065"
     CDS             complement(18619..19221)
                     /locus_tag="PLL88_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HOJ00968.1"
                     /translation="MAEVLPDEGGFFEIQISNKDFSHTFIQNKDFDSCVFTNCQMKET
                     TFRHCQFRDCTFEQCDLSLAKLEQCHFQHTTFHKTRLMGIDWTKLEWRKKALKPVLQF
                     SQCNLRYANFFACPLKGSDFTDCLLQEADFAEADLSTCDFQGSDLARARFLHTNLSEA
                     NFCGAQNYAINASNNLLKQTRFSLPEAVALLHSLDIILEE"
     gene            complement(19276..19821)
                     /gene="msrA"
                     /locus_tag="PLL88_05070"
     CDS             complement(19276..19821)
                     /gene="msrA"
                     /locus_tag="PLL88_05070"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOJ00969.1"
                     /translation="MNKTNDLQVAVFGNGCFWCTEAIFSQLKGVVSVTSGYAGGHTLN
                     PTYQQVCSDTTGHAEVVRITFDPLLITYADLLEVFWQTHDPTTPNRQGNDIGSQYRSI
                     ILYGDEGQKQIAQQMLNDLEERKVFPAPVVTEIVPLKEFYPAEDYHQEYYFLNKAQPY
                     CQFVISPKLEKFKKKFSEKLT"
     gene            complement(19805..20179)
                     /locus_tag="PLL88_05075"
     CDS             complement(19805..20179)
                     /locus_tag="PLL88_05075"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HOJ00970.1"
                     /translation="MNKMEPLTPEEEQVILYKATEAPFTGEYDDFFERGIYICRQCGA
                     HLFNSSSKFHSGCGWPSFDDAVPGAVQELPDADGFRMEIQCAACGAHLGHVFRGERYT
                     EKDTRHCVNSISMRFIPDEQNK"
     gene            20415..20912
                     /locus_tag="PLL88_05080"
     CDS             20415..20912
                     /locus_tag="PLL88_05080"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOJ00971.1"
                     /translation="MKKQFDQLDHKIIQALHNDARLSASQIARNLDANERTIRKRIDR
                     LVRLGAIRLTAIIDPEAFGYLTAVDIFLDVDADKEDEVIKEFLNTPDVSYIAFGEGTK
                     DLSIECRFKTNEDLREFIRKKIPTIEGVQIRGYSLVPRIIRNIDEWMPRDSDFSIERE
                     EEDDF"
     gene            20973..21986
                     /gene="ptcA"
                     /locus_tag="PLL88_05085"
     CDS             20973..21986
                     /gene="ptcA"
                     /locus_tag="PLL88_05085"
                     /EC_number="2.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR04384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050231 - putrescine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009445 - putrescine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine carbamoyltransferase"
                     /protein_id="HOJ00972.1"
                     /translation="MKLKHFIDTQDFTKNEMLDLITLIRKIKQADKQGVTPKLLEGTS
                     LAMLFEEPSTRTRISFEVGMAELGGHALYLKPGEIHLGVRESLSDTAHVISRYVDAIM
                     ARTLKHETMLDLMKNATVPVINGLTDYNHPTQVVCDVLTMIEHAPEGKKMEDFNVTFV
                     GDATNVLSSLMFICTQLGMNFTHAAPKRYQAPEAWMKIARENCEKSGGSVHVTEDPIE
                     AVKNADFIYTDLWWWVGQEAEIPDRQKAFMPRYQVNMDLIKKAPAHVKFMHCLPASRG
                     VEATDEVMDSEHSIIFDQAENRLHTEKGILVWLVYPRLKHASAEQKAVHAGQIVDFLK
                     SHF"
     gene            22026..23426
                     /locus_tag="PLL88_05090"
     CDS             22026..23426
                     /locus_tag="PLL88_05090"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HOJ00973.1"
                     /translation="MNASKTVVKEKPTGFKKVLRSLDMTLFTVCAILVMDTLAPSAAI
                     GPSSISWWLITLVLFFIPYGLITAELGTTYPEQGGLYIWVKKAFGEKWAARTTWLYWI
                     NVALWMPSVYILFAGMFAQLFFPEMGLWWQILIGIIMTWITVWIGSITLETSKWVPNI
                     GAIIKALIMVVIGVGAFIYAGKNGVANDLSFGNILPEWGAGLAFLPVIVYNFMGFELM
                     SSASEEMKNPGKDIPRAIIISGALIAAFYLLGTVGILMALPLDGLGLISGIIDTLRVL
                     LGESGIGGALVMVLGVAALYSFLANMVTWTMGANRTANEAAKEGELPTVFGKEHPVYK
                     TPVGAFIITGIVSTAVMVIYGFMAGSAEDLFWTLFAFSSMVFLLPYLVMFPAFLKLRK
                     IDPDVERPYKVPGGKLFITIMSVICEIFILQAVVFFVYVPGEPIDWAYATPVLIGVIL
                     TVIVGEVLIAVGKKKA"
     gene            23458..>23851
                     /locus_tag="PLL88_05095"
     CDS             23458..>23851
                     /locus_tag="PLL88_05095"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOJ00974.1"
                     /translation="MSCRIQSTPRKDGFRMPGEFEPHTGTWMLWPERPDNWRDGAKPA
                     QKAFAAIATAISQFEPVTMGVSQSQFNNARQMLPAAVRVVEMANNDSWMRDCGPTFVV
                     NGTLVRVVDWDFNAWGGLLGGLYFPWDLD"
BASE COUNT         5885 a         5557 c         6294 g         6115 t
ORIGIN      
        1 cttaggatct ttctatttgg ctcaccttta ggatctttct acttagcctt gacagttttc
       61 ttaaattatt attgtttatt tacttaaata tagtataatt ttttctttgt caatcacgta
      121 atgaaagcca tgagctgaag ttcatatttg gtcgcttcat taagaacttc agggagcaag
      181 tagcgaaacc ggtcgtcatt aaccggtttt ttttattaca attaaagata aggaggaaaa
      241 atgaaaaacg gatttcgatt cgtcggttta ttgccggtca tcccgatgct cggaatgtcg
      301 gcgtggtgtt gcgacaaggg tgcctcctta tgaatagcaa gacaaaaaag ccagtcggtg
      361 cgcaggcgat gcggctggga gagaggctgg tcggcgccgg ttatatcaca cgggagcagt
      421 gcaaagcggc gctcgcatat cagaaaatac acggcgacct gttcgggcat acgctcgttg
      481 agatggggat tcttacggcg cagcaactcg gcgatttttt tgccggtggg ggcatggcat
      541 tgtcgggaga gcggctggtc cacgccggta tcataacgcg cgagcagctt gtgtgggcgc
      601 tgaactacca ggaggagaac ggcgggcgtc tgggaaatgc tattgttgca ctgggatttg
      661 cgtctcggga aaccatcgat gagtttttca aaaacactgc ggtggcagac tatcgaaaat
      721 tgggcgatat gctcgtggtg cgcggctttt taacggcgca gcagctggaa caggcgctgc
      781 tgttccagcg caacagtgga gggcaactcg gcgagatctt gatttccatg aactttgtcg
      841 cgcccgagga tttgtacagg acactggcta cgcaaaagca gcttggacgc atcggcaaac
      901 gttttgattt cagcgcctcc aaaaaacttc cgtatcatgt ggcactcaaa cataacgcga
      961 tcatcatcaa caaccggaaa gacgcgtata ttctggcagt acatgacgaa ctaaatgata
     1021 aagcaacggc agagattcaa tcctttttgg ataaacccgt cgagcaagtt ttggcaggca
     1081 tgaatgaaat cgaggggtta tgggaaatgg tgtaccaata cgaacagacg gaggacagcg
     1141 tttacaagct gtacaccgac cagccgggca attcggctct gctcacgctt tccacaggtc
     1201 agaaagtaac cggaatctgt cttttgggcg tgttggtgct ctgcctgctc tttaactatg
     1261 tgaacacgtt gcttgtcctg aacgttgtgc tgcagtgtct gtacgccgta tttactgtgc
     1321 tcaaggttat aatcctgatc agaggactga agaggcacgg acaacttcgc tacacaccgg
     1381 aacagttgga cgcaatcgac gagagagatc tgcctatata caccattctg gtgcctatat
     1441 ataaggaagc taccgttgta gaaaatctta tgcaaaggct cgacgcgctc gattatccca
     1501 aacacaagct cgatatccgc atactgctgg aagaggacga cagcgaaacg ctgaaagctt
     1561 tccaaaagct gggcgtcccc agccactata cgttgattgt cgtgccgtct accgagccgc
     1621 ataccaagcc aaaagcttgc aattatgggc tgatacgagc ccggggcgaa ataaccgtga
     1681 tctacgacgc cgaggacatg ccggaggcag atcagctcaa gaaggttttt ctggcgttcc
     1741 gagaattgcc cgaatcgtat gtctgcatac aatcaaagct taattatttc aacaacgacc
     1801 agaacatact gacccgctgg tttacgcagg aatatagcac atggttcgat atattgctta
     1861 ccggcgtcat gtccatagac atgcccatac cgctgggagg cacctccaat cattttaaaa
     1921 cccgcttttt gaaggaaatc ggcgcatggg atcctttcaa cgtcacagaa gacgcagatc
     1981 ttgggatcag gctgtacaag atgaattatc atacagctat gctggattcc tacacgtggg
     2041 aggaagccaa ctccaatgtg aaaaactgga tacgccaaag gtcgcggtgg atcaagggct
     2101 atatgcagac ctggctggtt catatgcgca agcctgccaa actgtaccgg gagcttggac
     2161 taaaaggttt tataggttat caggctatgg tgctcggaac tccgcttctg cctatcctca
     2221 atccgttctt ttgggtgatg ttaattctat ggtatgtgtt caaaccgccc tttatagggg
     2281 cgctgtttcc gggagcgttg tactacatag ccgctgtaca gttctatttg gggaatttta
     2341 tatttgtcta ttccaatata ttaggcacct acacggtgat acgcggcagt gagctgacgg
     2401 gcaagatgca catccgttat tcggtgctgc cgtctgggat cctgttgcct gtctattggg
     2461 ttttgatgag cgttgccggc tacaaggccc tggttcaatt gattttaaag ccctcttact
     2521 gggaaaagac gcaccatggt cttacggaac agaccgtgct gaacacgacc tcgacgtaaa
     2581 aaataagtat cacaggtaaa ggttggaagg aaacatgaaa aatataaaaa aacgtatctg
     2641 catcatattg gcgctgctgt gtatgtttgg gggaacagca ctgacgcagg caccgtcgtt
     2701 tgtccgggaa caggaagcaa ccgatgtgcc tgcgcaagag cagccatccg ctgaggaaca
     2761 gacggtgggc acaaagttgt tccttttcag ggaagatcga accctcacga tgcccaaaag
     2821 caccgtttcg tactggtttg tggttcctca gggcatttcg gtcgggcaat cggctgtatt
     2881 gacgttgcac atttcgtatt cggcgacact gatagaagcg ttgtcgtcgg tgactgtttt
     2941 tatcaacgga acgccggttg gcacgcggag gattgttgaa caggcgggcg atgagtttat
     3001 ctggacggtc gaattcgatt cgtcgctcat tcgcatgaca ggatataacg agctcaaaat
     3061 cacgacggta caccgctcgc ttgaaggcga ctgtgcggat gttgataatc cgtccaattg
     3121 ggtagtggtg cacagcgatt ccttcttgag cctggaggag tccgcagcag tggatccgct
     3181 tttatcctca tatttcgcgc tgtactacga tacgttcgct gagcctttta caattgcagg
     3241 tgactatgtg ctcagcgaca catccgatgc agatatcatc tctgcgatgc tcgggctgtc
     3301 gaacgccagc ggaatgtatt actctgataa agaatatgtg cggctgaacg tgtatgacag
     3361 ggacggtttt tcggtagaca acaagaacaa aatattcatt gaatcgcagg aagcacccgg
     3421 agaatggtcc gaagatctgc tgccgctacc gcagcaggcg cccgtgcagg atggtgccta
     3481 tgtatccatt gcgggattta ccgaggaata tccgttttat aagctattcg tgtcgggcag
     3541 tggacggagc ggactgaaaa aggctacagc gtttttaagc aaccgtttgc tcgtcgagca
     3601 ggcagaaagc ggcgacgtgc tccttaattc cgatatcccg gttgacaatg tgcaaagtag
     3661 cgtagcgttg aacgaagatg gggtttacag ttttgcagat tacggctatg cggacatcac
     3721 catggcaggg gcatttcatc agagtgctta tctgtcgttt gcgcagccgg gcggcgtaag
     3781 cggcggcgag ggctcgtatc tggaactgcg gttccggcac agcgccgtgc tgctgtctga
     3841 ccgttccgtg cttaacgttt acgtcaacaa cgtggctgtt tctagcgtca aactctcgcc
     3901 cggtaacacg gagactggca gtctgattgt agagttgtcc gcaagtgcgc tcacacagcc
     3961 cgttatcaac gtgaccatag aaacctatca ccatttgggg caggaggtag actgcataaa
     4021 ggactattac gacgtggcgt ggacggtgat tgatgctgaa aactcccgca tcgtttttga
     4081 aaaaagcgaa aatgcgctga ggctttccct gaaaaacttc ccgtattttt cgagcggcaa
     4141 tacatccaca gtgtcagtat atgtgccggg ttccgcggga aaggatgcgc ttgaggcggt
     4201 tgcagcgctt gcgataaggg cggggcaaaa cactcggatg gcattcgaat gggaaattgt
     4261 tcccagtgcc tccggtctca gcagcgttgc aggagatatc gtgattattg ccgaaaaaag
     4321 cgatgcggcg cttcccgaag aaattgctga aaaattagct attacttctt ctggcacagg
     4381 ctatgtcgta agggatgcta cgctgcctgt cataccggaa acgctggcag gcaaaacgat
     4441 cattcaggtg atacgctcgc cctggaattt ttacagtagc atttacgtta tcatctacga
     4501 cggcgctgag ggacttgcga acctcgaacg ttttcttggc gaacgcacag aacttaatca
     4561 gcttgacggt cagctttgtg tggtgggcga cgaaacaggc gtacaaacgt ttaccgtttc
     4621 tcctgcgtcc gacggtgagg atagcgtacc cgtggatcgg ttggatcatc tgatcgcgca
     4681 agccgaagag ttcaccggtc tgcccgcatg gctgatattc agcttgttgg cagttgttgc
     4741 ggttctcatc gtcattgtca tagtcatggc tattaaaacc agcaagcgcg acgagtatca
     4801 agatgccgcg gaagaacaca ggaaaaagca ggattttttc aaggatgagt agctaggagg
     4861 ctgtcaaaag acgtgggttg tgttggatgg ctgccgtcag gggcgggacg gcatgtcgtt
     4921 cgctgaagca atgaggagcg agtgaatact gttcaaaaaa agaaatccgg gtattggggc
     4981 tggacatggg taatgttttt tgtagttcta gccgcggaaa tagtgggcgc ttatatactg
     5041 ctgtacaaaa agggctatat tttgaccgac gcaatagcca gaacagccaa tgcctactat
     5101 gtgttgtata tcgatccgcc caagttggct tccatcgggt ttgtatggaa tccacttccg
     5161 agttttttgc agattcctat tctgctgttt aaggatctat ggcccccgct tgcgacccac
     5221 ggtgttgccg gcggtatcgt gaccgcgttt ttcgctgcca gtaatgccat gatcctgttt
     5281 cgcactttca aacactactc catcagcacg gttctttccg tgatattggt gttgttgttt
     5341 gctttcaacc cattcatgtt ctactacggc tgtaacggca tgagcgaaat gccgtttttc
     5401 acgaccataa tcgcatgcgt tgtgggtctt tgccggtggc tggatgagcg cgataaccac
     5461 aagctgctgg tcatgatggc gttctctctg gcgttggggt tcttgtgtcg ttatgaagca
     5521 tttgccatca cgctggggtt gggcatatcg ctgctcatcg tggtctttgg catgaaggac
     5581 ccttcttccc cttttgtacc caagcccttg cgcatgaagg cagaatacgc catggcgaca
     5641 gggctggtgc ttttcttgcc gattgtgtat tctgtagtgg tgtggatcat actcaattgg
     5701 tccatcatgg gtgacccgct ttactttctt acatctcctt attccaatgc tgcttttaca
     5761 tcctacactt taagctccga ttttataacc gctatgtcct cgcccgtaaa agcgcttctg
     5821 ttctcgctgg ggaggatgct tcccttcctg ccgctgttta ttgtgatcac gattggcaga
     5881 atactttcaa aacgactgtt caaacctgat tactgtgtgc tggtagtgat ggttggctgt
     5941 atttccgcgt tccattttct catgctgctc agcggaagct cgtttggctg gctgcgcttt
     6001 tcctcgtttt cattgcctat ctgcgcggca tggctgccgt atgagctttc gcggctgaag
     6061 ggaacggtca aatacctatc ggttgctgct gctgtcgttg cgttcctcgc atcagccttc
     6121 atcgtcgtgt cttactttgg ctccctgaca cgcgctccgg aggaatattg ggctttcgta
     6181 cagacagacg tggccgacgc gacagttcag cgcgctgcag cggagcgcat caacgaggcg
     6241 tatagcgacg acgtgatgat gatggacgat tctgcgatga gcgcgctggt cgtaaatctc
     6301 gatcatcccg aacgactgat tatcagcacc tcggatcagt tcaacgatgc gcttgaagca
     6361 ccatggaggt acggtgtccg ctatattgtg gttgccgacc cctttggtgt gggctctcgc
     6421 gatgtgttca atatgattta cccgagtttg tatgaagaag gcgccgattg gtgtgtggaa
     6481 cgtgaggatg tgggaggctt taaagtattt gaagtgctgt attgagaaaa gaagtgcaac
     6541 tgagctaatt gcgaataccg ccgccctaaa agccatccag aacataagat ggctttttgc
     6601 ttaaagaagc gtgaaatcca tgattccatg atgtgaccgc ttttgatata cttgggggaa
     6661 ttatcgggag gacggtatgc agtaccgacg aatagtagtg aagatcggca cttccacgct
     6721 taccggcggc acacgggcta ttcggcatgc gcacattgtc gacctggcaa agcagatccg
     6781 ctcgatccgg gaagaaaaat gccaggtcat ccttgtttca tcgggcgcaa ttgctgccgg
     6841 aagggaagcg atgagtgagg cgagtttggg gaaacacatg cctgccaagc agatgctggc
     6901 agcagtcggg cagccgcgct tgatggctat ctacgagcag gtgtttgcca tgtatgatgt
     6961 cagggtgggg caggttctac tgacccgtac cgacataacg aaccgcaaaa gttatattaa
     7021 tgcccgcggg accatggaag cgctgttgaa caacacaatc ttgccgattg tcaacgagaa
     7081 cgacaccatt gcaaccgatg agatcggcat tggagataat gataatctgt cagccatggt
     7141 ggcaaccatg ctcgaagccg acctgttggt tctgctcacg gatactgatg gcatttacga
     7201 gggggacccc acacgggaga atgcgaaatt gatccgcgaa gtccgatcgg ttgagatacc
     7261 tcagacggtg tggaaagcgg tgggcggttc cacttcaggg cttggaacgg gcggtatgct
     7321 gaccaaagtg caggcagccg agatcgcgcg aagaggcgga accaccgtgg ctgttgtgag
     7381 aggagcggag ggtatcttga cgcgaatcgt aggcggagaa aaacccggaa cctggatcta
     7441 cccaaccatg gatgcgctgg aaaaccgcaa acgatatctt ctgaccagcg ccgtaaaggg
     7501 ttgcggggtt ctgatcgata gcggggcaca aaaagccttg cacaaagggg ggagtctgct
     7561 gccggtgggt gtgaccggcg tgcgcggtga attccaacgc ggtgacgtca tttatgtttt
     7621 tgcttttgat ggaaaaccat gtgctttggg tcgttgcgcg tattcttccg atgaaatcct
     7681 gtgcatcaaa gggttgcaca ccgagcagat tgaaccgcgg cttggatata tctatacgga
     7741 tgaaatgatc catcacgatg atctggtctt attgcagaac catggagcgg aaaatgaaaa
     7801 cggtgcttga actatgcaga caggcgagag aagtttcttt catgttgccg caatgtgcgg
     7861 cgcaacaaag aacacaagcg cttctcgctt tggctgatat gctgatcgaa ggcagtgatg
     7921 cggtcatgcg agagaacaaa attgacatcc aagaagcgtg taagcaaaag gtatcgaatg
     7981 catcactgga aaggcttgtt ctttcgcggg aacggatcga agaaatggca gaaggggtga
     8041 gggctgttgc cggcttgccg gatccgctgg gacagatctt cgacgaacgt gatctgccga
     8101 atgggttgca cattcgtaaa caacgcgtcc ccctgggtgt gctggctgtg atctatgaat
     8161 cacgccccaa tgtgaccatt gatgtggcgg cactgaccat caagtcggga aatgcggtca
     8221 tcttgcgagg cggcagggaa gtggtgagaa ccaatgccgc actggtggat ataacccacc
     8281 aggcgttgaa aaggtcgatg atcgctgaaa cttccgtgca atttctggac gaccctgagc
     8341 ggagattggt ggatgagctg ctaaccatgc atgcatatgt agatatgctc ataccgcgtg
     8401 ggggagctga cctgcatacc tattgccgtg aaaacagcca tattccggtc atcaccggcg
     8461 gaatcggaat atgccatctg ttcgttgacg aatcggcaga tctgcaaaaa gccatagaag
     8521 tgatcgtcaa cgcaaaagtg caacgcccca cggtgtgcaa tgcgttggat acgctcttga
     8581 tccacgaaaa gatcgctccg gtttttctgc ctgtattggt cgaaaggatg caggtggaag
     8641 gggtggttct ccatcttccg gcagaactga tcgaacgata tgccttcacg caaaatgact
     8701 tactcgtgcc tgcgaaagag ggagactacg atcgcgaatg gttatctttg catatgaaca
     8761 ttgacgtggt gccagatatc gggtctgcta tttcttttat ccaccagcac agcacggcgc
     8821 attccgatgg cattctcacg gaagatcagg gaaacgccgc cctcttcttg ggcaaggtgg
     8881 actcggcagt ggtgtatgta aatgccagca cacgcttcac ggatggcgcc caattgggat
     8941 tgggggctga agtggctata tctacccagc ggctgcatgc gcgaggaccg atgggattgc
     9001 aagaattgat gacatataaa tgggtcgttc agggagatta ccatgttcgt gacaagtaac
     9061 ctggaaggac tgcgattggg aaaagacatt cacgtaaaag ccaccgcaag cgcggtggct
     9121 ttttctacat aataataaag aattgcatca atgagacggg cttagaaacc tttgagtacg
     9181 tttccgagcc atagcaggac gaatcctgcg gctaacaacc agaaggtaaa gccaccgatc
     9241 actggaagag caacgaagtt accaatgatc cccagaacgg ctaagatggt tgccagccaa
     9301 aaaactaatt tggttggagc gctgagtttc atttcaatct cctttctcta gttttcttat
     9361 gttaagtata acgaaaaatt ggcaaaatgg tttcgaaaaa ggagaattgt atttgttcag
     9421 ggaagacgaa tatcacgtac tggcaagggc aacctaattc gtgatcttga tctcaacttc
     9481 tttgatttcc tcaacgccct tgagcgcttt cagcattccc aggatggtct ggatgatgat
     9541 ctcggctgga aactgggtta ggggtacttc ctttccattc acttttaaga taatcgattg
     9601 catcatttgc tccttatttc atgatttctt tattcttgat ctgagagaaa agggtgtcat
     9661 aatctccggc gtacgtgagt accgtgttct cacgcaaagg catgtctccc aagcgaacct
     9721 tggggatcca tgcttccaga gcaccttcga tgaaaatgta ctcatacgct ttgttctgcc
     9781 caagcagatc aacgaccgct ctggaagaaa gcggttcttt gattaaaaat gcggtttcga
     9841 tagccgaaga gaaaaccacc gtaccagcac ccgcctgacc gtaaagaaaa gtgtcctttc
     9901 cttcggtgtc gatgctgatg gctttgtcgg aaattttcac cacagccacc tgaaagcctt
     9961 ctttaaccaa ttgggctacg atcttcttga ccaaataggt cttgcctatg ccagaacgac
    10021 cgtaaaaacc aagaatccat ggagttggct ggatgtcctt cataaggata ttgtaatgga
    10081 gaaaatgaaa gggagttccg tggaaaattg aagcagcgca taggagctct atacgctgct
    10141 tgttatgatg gtgtttatag ataaaatatt cgatcgcgat attctttgag cagttttgcg
    10201 ttgtgctctg cgcctccatc cacattggct gccacgaaaa ccggaggggt gatgccgcgt
    10261 gccagcagtt cttcgatggt ggcggcggag aggcagtgca tgacggcgct gctggtgatg
    10321 ccggatgcgg aggcgaattt gggttcgagc ccgtcagccg agagcaaagc gtctcccttc
    10381 tctgccttgt tatcgatgac cacatcggcg aaatcgtgca tctttttgcc ggaggggtgc
    10441 cttgaggtaa cgccttccga atatttccgc gaggtgatgc ctatgacggt gatgccccgt
    10501 tcgcgcgcga tctttgccat ttcgattggc accgcattcc gaccggaaac cgaaataatg
    10561 atgagaacat ctccctgttg gatgggctga ttatcgagga tcacttcagc atatccgctt
    10621 aacttctcga tccctgatgt catggtggtg gggtggacat ccagatgtgc aatgccggga
    10681 gcgtaaatgg ggttgatgag cataagcccg ccagcccgat ataccacttc ttggatgggc
    10741 aggcaagaat gggtgcaacc gaagccgaag atgcgtttgc cattttggat ggcatcagcc
    10801 atcaggcgtg cagcttgatc gatcgctccc tcttcttccc tatccacttt ttgaagaagg
    10861 gaaacgaccg cttgaatata ttcatgggtt aatgtagaca ttctttactc caaatgctat
    10921 tttgtataaa ccaaattacc acccacgatg gttgcgacaa cctggtatcc agcgtcgaag
    10981 atggcgaggt cggcatcata gccaacccgg atatatccgt ttttgcgtgc gaaatgcatc
    11041 gcttctgccg gcactctggt agccgcgggg agaacatgcg gaaagtccgc gccggtgaac
    11101 tgcatgacat tgcgcaccgc cgcatccagc gtgagggtag aaccggcaag actgccgttg
    11161 gctattctgg cggtgccgtc gatcacggtt acttcctgac ctcccaggtc gtattttcca
    11221 tccgataagc ccgtggcgcg catggcgtct gaaatcagaa tggtgcggtt gacacccttg
    11281 caggcgagca cgagtttgac aatcgcggga tgcagatgca cgccgtctgc gatgatctgc
    11341 gcgaagatgc gctcatcggt caaaacgccg ccaacggttc cgggttcgcg atgatgcaat
    11401 ccctgcattc cgttgaacag atgggttgcc tggcgcacgc ccaaatcggc tgcatgaatg
    11461 acctgttcat atgaggcgct gctgtgaccg attgccagtt caattccacg ctcaacgcaa
    11521 gccctgatga attcatccat tccgggtagt tcgggtgcga tggtgatcag cttcacccat
    11581 tcattatcca accatgggtc gtactcgctt ttcaagggag gtctgaaata cgcggggttt
    11641 tgcgcgcctt taaaagcgat gttgatgtat gggccttcca cgtgcgctcc cagcagcctg
    11701 gcaccttcca attctgcctg cgattgttcg atggcgagca gagcacggtt gatgctttct
    11761 tggctgtttg tgaccgtggt agccaaaaaa gatgtcacac catgggcggc aagaaacttg
    11821 ctcatggcgt tcaatgcatc gtgagatgca tccatggtgt cggcgcctac gccgccgtgg
    11881 gtatggatgt cgatcaagcc ggggatgacc cagtctctcc tggcatcgag gattgcatca
    11941 gaaggttttt cagataattg ggtggggtaa atatctttga tcctgccctg ttggatctca
    12001 atcgagtggt cgggtagaac ctcgtcaggt gtgcaaatat tagcgtggat gattttttgt
    12061 atcatggtgg tattataaaa gaaaagagag gagaatagcg ctctcctctc tttcttttgc
    12121 caggttttat ataccgaata atttggcgat accccaaatg ataatgccgg ggacaatcgc
    12181 atcgggaacc gtgaagccaa ggctcaccgc acccatgctg gtgaggtcgc cgaacacctg
    12241 gaacttataa gcaaagctga tcaacagcca accaacgaaa ccccacaaga aaccacccaa
    12301 ccaggcgccc ttgcgcccgc cggtggcatt tccaaagatc gctccggctc cgctggcgaa
    12361 gaaagctgcg atcatactgg gcagcggcac cggccaaccg atcagggcta aaaggaccat
    12421 gccaacgacc tgaccgatgg taccagagat caagccgaag atcagggcgt ttggagcgta
    12481 ggggtagata accgggcaat ccagagcggg ctttgcaccg gggatgagtt tttccgcgat
    12541 acctttgaaa gccggggtga tctcggcgat gagcatgcgc accccttgca gcagtaccaa
    12601 cacgccggcg gtgaaattca aggcaatgag aagcgtgtaa acgatccagt tctgcccacc
    12661 actgatctgt tcttggaatg cggcggtacc gatctgattg agggcaagac cggcgccgat
    12721 gaaggctacc agaagcatga taatggaaat cgaaacagcc atatcgcgca ggaagttgaa
    12781 ggaatctttg atcttgatgt tttcggatga gtcttccggt tttccaacca gtttgcctgc
    12841 ccaggctccc agcatattta aaagggtttg cccatgaccg aaggcgatct catcgctgcc
    12901 ggtgattttg cgcataacgg gttgagcgag ggcgggcatg agggtcatgt agaagccatc
    12961 gacgatggat gccagggcga cgactgccca ggtggaaagc ccgaaggagt ggaacaggat
    13021 ggcgaacgcc cctgcgtgca cccagatcat atgcccggtc agatagatat agcgatactt
    13081 ggtaaaacgg gataccagaa tatgcaccac ataaccgaac aacatcgtcc acgcgatctc
    13141 ggcgccaata ttggcgaggt ttgcgctttg caccgctcca acgaccgcct catcgtaggt
    13201 cacgaaagtt tcaaaaccac ccgccgggat ccctttttca aagaaaacct gaattggtga
    13261 aagggcactg atcaaggctg tgattccaat actgatgatt aactgtccaa gaattgtttt
    13321 tagcgttccc ttgaatacat ctcccgcaga ttttttctgg agaagaagac cgatcaatgc
    13381 gatgaaaccc agaacgatcg ctggtaatgt gatcaaagag agtattgcat caaatactgc
    13441 cattttatcc tcctagaatt ttttatattg ggtgaagata aaaaataact tatccaggaa
    13501 cctcctttct acgaaaaaaa attatgagag ctctcgaatt gcagtgagga ttttttcttt
    13561 aagttctgct ttgtccacca ggttcttgat gagaacgatc tttgcagggt aatcgcgcaa
    13621 atgaggttcc atgtccacgg gagccacaac gatatctgct ttgttgccct tgaatgttcc
    13681 aaggtcccag ggacttgttt cggctttgat cccttctgct ttcaatattt catcaatgaa
    13741 caatttcaac atcatactgg tgccaaaacc cattccgcat aacgttgcta tgaccatttt
    13801 ctctctactt tcttgttttg atatattcaa aaattttttc ctgcaaagtg tgtgaatctt
    13861 cactgtttcg caagacttga agaaaatcct cttttgccaa aaaaccagcc aactctgcca
    13921 gcgcttcaat atgatcgttc ttatctttgg cgcccaaggc aaacaccaca tccaccggat
    13981 cattggtgga gtgaccaaat ttcaccgggt tccgcagggt gagcagggaa agacaagccc
    14041 gttccacccc atcttccggt cgcgcatgga gcagggcaat accaggcgca atcacgcaat
    14101 atggtccaag ctcgtctatt ccttttttca tcgcttggat gtactctgat ttgatcgctc
    14161 ctgcctcaac cagcatgtca ccagctttct ggatgacctc ctctctggtt tccagtgaaa
    14221 gcttagctct gatggcactt ttgatcaaca gacgttctag ttgcatttct cattcatcct
    14281 ataactcaac gactgcagag agattctccg gtttatccgg atttaatcct tttgccatgc
    14341 ttctctcgta tgccaacaat tggatgagag ggagataaaa aggatatacc cacggtgatg
    14401 acaccttttc tccaaagtcg aagagataat cgatcccctg cggttttcca gttcctttgc
    14461 cgatcgccag cgttcgtccc cccagctttt gcatatcgta gaaaaccggg agctcaaagg
    14521 aaatggtatt ctgactggta aatccgacga tcagcgattg gtgattgacc atggacatgg
    14581 gaccatgcct gaactccaga aaatgaaaag cctcagagta gctcaaagac atttctttca
    14641 tcttcaacat gatctcttga gccagtccga atagcggccc gtttccaaga aaaaagaacc
    14701 gttcgatatc caggtctctg ccaatctcgc gggcggtggg ctcgttttct gacaggaact
    14761 tttgtgctga gcggtgtatg gaggtaatca gatcatctga gatgttcttc tcgcccaata
    14821 gaataacagg ccacagcatg ttgctgaacg aacgggtttg cgcgatgctc tgttccatgc
    14881 ctgccgtggt agtaattgcg agtggagaca tctttgccag agaactgtcc ttgtagcagg
    14941 tgatggcaag ggtgctctga ccgtgttttt tcaaaaagag ttccagggct tgcaatgttt
    15001 cggtggtttg ccccgagcgg gagatggcaa taaataagat ctttttaaag ggcttcaacc
    15061 attgatcccc cgcataccac aactcggaac tgggcagagc catggctgga acaccggtgg
    15121 tgcagtgtaa aaagcgggct gcccagatgg aaagatagta tgtcgatcca cacccggtga
    15181 tgacgagctg atcataatcc tgaacgtgcg gtagttttcc aacccaatca gattgtagat
    15241 agcgcagtgt ttctttccat gtttctggtt gcgaaaggat ctccgtttgt gtgaacttgc
    15301 ctctgtcttc ttgcatgaat cctgttcatc tccttgaact gttaaacttg cattacctcc
    15361 acgccctgtt cccgaatggc tcgcaccaga tcatcctgcg cgtcggtggt ggtgataacg
    15421 gtttttgctg ccgtgatcgg agcggtgcgg ctggtggaaa cgtagccaat cttggtgtgg
    15481 tcggcgacga tgatgacatt gtcgctgatg ccaaggatct tgcgatcggt cattaactcc
    15541 tgcggatcct cactggtgag cccgaagcgg ggatggaccc cgcgcattcc tacgatcacc
    15601 ttttcaacgc gcaagtcttc aataacccgc tcggcaaaat gtccgatcat ggattgctct
    15661 tcacgccgca gaaagccacc gatcccgatg acgggcatct tgccattcgt gctcagctcg
    15721 ttgatgatgg tgatggcgtt gctgaggacg gtgatatcgt ctctttcata aagttctctg
    15781 gctacgtaca gtgtcgtagt gcccgagccc aaaaagatga cttccttttc gtcgatcagt
    15841 gccgccacgg ctttggcaat gcggatcttt tcatctttta aaagttccat gcgcttgatg
    15901 gtagggggct cgctatagtt gggggattta gaggggagta cagccccacc atgcgttctt
    15961 tttaattgat tgcttttctc gatctcaaag agatctctcc gaatggtcat tcttgaaacc
    16021 tgaaagcgtt cagccagttc actgaccgct acgcttccat tccgacccag ttcctgaatg
    16081 atcacttgct gtctttcttc ttttaacatt tggctccttt tcataatatg ttacgttgtg
    16141 ttaataagtc aaataatagc acaaaaaaga acacaaaaaa tgttcgaatg tgagttatac
    16201 aatgagattt tggggaaatt aattggaaat atgcaaaaca aacaaaagcg aacatctcaa
    16261 aaggcgcgcc acgtgatatg atcttcaaag agaaagaaaa gaaagagttc aaatggacac
    16321 acgctcaata ggcaagatcc gtggattaca gcaatgtgcc actcagcatg gcaaatttgc
    16381 gatactggct cttgatcacc ggaacaacct gcgcaacctg ttgcatcttc aagagcaaag
    16441 ccctgacaca gatgtggaaa tgacggcttt caaattcgac gtggtgcgag cgcttgctaa
    16501 aaattgcagt gccattctcc tggatccggt gtatggcgct gcacagaatg tcttctctgc
    16561 ggctctgcca ggaaactgtg gattgctggt ggctgtggaa gaaaccggct ataccggcgc
    16621 tccggctgcc cgtgaaagcc agctattgcc agagtggggt gttgccaaag tcaagcgtat
    16681 gggtgccagc gcggtgaaac tgctggtgta ttaccatcct gattcttcca cccatacgca
    16741 cattgaggat ctggtgcgca gggtggcgca agattgccaa acgcaagata tcccgctttt
    16801 tttggaagtt ctttcctacc cgctggatgc cggtaagaaa aaactggagg gaaatgaaag
    16861 gagcgatgtg attttaagat ccgccgctat actcaccccc cttggggtgg atgttctaaa
    16921 agcagaattt ccgttggaca tctatgcaga aaaggatttc cagaaatggg aggttgcttg
    16981 ccaggaatta acccgcgcga gcgcgacacc ctggatcctg ctttccgctt ctgttgagtt
    17041 tgaggttttt atgcagcagg tcacggcggc ttgcagggca ggtgccgcag gggtagccgc
    17101 ggggcgcgca gtctggaaag aagcgactga cctgaaaggg atcgagcgta cgcggttctt
    17161 gcaagatgtt gcttgtcagc gcatgggaag gcttaccgca ctggtagatg ccctggcagc
    17221 cccctggttt gcggggatgg agccaacccg gatcaaccag gattggtata agaagtaccc
    17281 tgagaagtga tgtgtgacat gcacaaaaac gggttctcga gcggcgagcc acttcaaaag
    17341 ataatccgca atcacaaaca gggacagcct gtggggatct attccatctg ttcggcacat
    17401 cgtgaagttc tgtgttctgc catgcaatcg cagaaatcgc gggatgattt attgttgatc
    17461 gaatctacct gcaaccaggt caatcaagag gggggataca gcggcatgac cccagaaaga
    17521 ttcgttgttt atgttcaaga gcttgcccgt gaagtggatt ttccgaccgg gcgtattcta
    17581 ttgggtggag atcacctggg accgaaccca tggaggagag agccggcgtc attggcgatg
    17641 ggaaaagccg gaaaaatggt gcaagcttac gtggcaagcg gttacctcaa gatccatctt
    17701 gacgcctcca tgccctgtgg ggatgaggct gcactttctg atgaaaccgt tgcagagcgc
    17761 tctgctcaac tctgcctgct ggcggaaagg gcagccagcg agagtggact ggctaccctc
    17821 ccgctgtatg tgatcggtac ggaggtgcca tctccggggg gtatggctgc caaccagaac
    17881 gaagtgtgtt ctgtgacaag ggttgacttt gcagaacgca cggtagaaac atttcaaagg
    17941 gttttccatc gtcacggtct ggaagaggca ttccagcgca tcatcgcgct ggtcatttct
    18001 ccgggggttg aattcaatga taagcagatt ttcgcttacg accgttcgag aacagccggt
    18061 ctcagggaat tcatcgaaac gatcccccat atggtatatg aggctcattc tactgattat
    18121 caaaccccgc aatcgctcaa acaaatggtg caggatcatt ttgcgatcct gaaagtgggt
    18181 cctgcgctca ctttcgcctt tcgtgaagcg gtttttcgcc tggcggcaat ggaagaggaa
    18241 ctgcttggat cagataagga gagccggtct cacattgtag atactttgct ggaaacgatg
    18301 caaagtgacc cttttcattg ggaaaaatat tacacgggtt ccgaaagtgc gatccgtttt
    18361 gcattgaaat acagtttcag cgatcgtgta cgctactact ggggatatcc ggcagtgcag
    18421 aaggcgtatc aaaagttgat aacaaacctt caccgaagaa ccctgccgct ttccctgatc
    18481 agccaatatt ttccccatct gtttccatct atccgcgatg ataccgtaaa agcagatcct
    18541 gccgccctga tcctggcaaa tgttgctcag gtgctcaaac actaccaaaa cgcttgtggc
    18601 tcaatatagg aaaggaattt actcttccaa aatgatatcc aggctatgca acaatgccac
    18661 cgcttcggga agactgaacc tggtttgctt gagcaggtta ttggatgcat tgatagcata
    18721 attctgtgcc ccacagaaat tggcttcgct caagttggta tggagaaacc gtgctcgagc
    18781 gagatcggaa ccttggaaat cgcaggtgct taaatccgct tctgcgaaat ctgcttcctg
    18841 tagcagacaa tctgtgaaat cggacccttt taaaggacac gcaaaaaaat ttgcgtaacg
    18901 aagattgcat tgggaaaatt gaagtactgg cttcaatgct ttttttctcc attctaattt
    18961 cgtccaatcg atccccatca ggcgtgtttt gtgaaaggtt gtgtgctgaa aatggcattg
    19021 ttccaatttt gccagactca ggtcgcactg ctcaaaagta caatcacgaa actggcaatg
    19081 tcggaaggtg gtctccttca tctggcagtt ggtgaagacg cagctatcaa aatccttgtt
    19141 ttggatgaag gtgtggctga aatctttatt gcttatctgg atctcgaaaa aaccaccttc
    19201 atccggaaga acttctgcca ttgtacacct ctatgatgct actaattgta atggcagttg
    19261 tttgacccgc ttgagctatg tcaatttttc actgaacttc tttttgaatt tttccagctt
    19321 gggggagatc acaaactggc aatagggctg cgctttattc agaaaataat actcctggtg
    19381 gtagtcttca gccgggtaga actcttttag cggaacgatc tcagtaacga ccggtgcagg
    19441 aaaaactttc cgttcttcca gatcgttcaa catttgttgg gcgatctgtt tttgcccttc
    19501 gtcaccataa aggatgatag agcggtattg gctgccgatg tcgttccctt gccgattggg
    19561 cgtggtggga tcgtgggttt gccagaacac ctcaagcagg tcggcatagg tgatcagtaa
    19621 ggggtcaaaa gtgatgcgca caacctcggc atgtccggtg gtgtcgctgc atacctgttg
    19681 gtaggttggg ttgagcgtgt gcccgccggc gtaaccggac gtgacggaca ccacaccttt
    19741 cagttggcta aagattgctt ccgtgcacca aaaacatccg ttgccaaaaa cagctacctg
    19801 tagatcattt gttttgttca tcggggatga acctcatcga aatagaattc acacaatggc
    19861 gggtgtcttt ctccgtgtat ctttctccgc gaaacacatg accaaggtgc gcgccgcaag
    19921 ccgcacattg aatttccatg cggaagccgt cagcatcggg caactcctgc actgcgcccg
    19981 gaaccgcatc gtcaaagctg ggccaaccgc agcctgaatg gaacttgctg ctggaattga
    20041 ataaatgcgc cccgcactgg cggcagatgt agatccctct ctcgaagaaa tcatcgtatt
    20101 ctccggtgaa aggcgcttct gtggctttat acaggataac ctgctcctct tcaggggtta
    20161 atggttccat tttgttcata atcaactcac tttcactatg aatagagttt ataaaggaag
    20221 gagggcaaga ggtattagcg aaatgttaat ttacgacgta cttttttcct ggaattgatc
    20281 tcataaatca gtttcataga ggatgatgaa tgtcatgaat ttttcagaga tttgattaat
    20341 cccttgaaat acatttccaa tctcgctata ataattccga aagggaataa tgaagttccg
    20401 aaacggaatg cccgatgaag aagcaatttg accaactcga tcataaaatt atccaggctc
    20461 tgcataacga tgcgcgcctg tcggcttcac agatagcccg caatctggat gcaaatgaac
    20521 gcacgatccg caaacgcatc gatcggttgg tgcgcctggg cgcaatccgc ctgaccgcca
    20581 ttattgatcc ggaagctttc ggatatttga ctgcggtgga tattttcctc gatgtagatg
    20641 ctgacaaaga agatgaagtg atcaaggaat ttctaaatac cccggatgtt tcttatattg
    20701 cgtttggaga aggtaccaaa gatctctcca ttgaatgcag gtttaaaacg aatgaagatc
    20761 tgcgtgaatt catccgtaaa aaaataccca ccatcgaggg tgtgcagata agggggtact
    20821 cgctagtgcc acgaatcatc cgcaacattg atgagtggat gccgcgagat tctgatttca
    20881 gcattgagcg ggaggaagaa gatgatttct aaatatccgg cgacgagccg gatatctttt
    20941 ttaaaaatta agaattccga aaggagcata gcatgaaact aaaacatttt atcgatacgc
    21001 aggactttac caaaaatgaa atgctggatt tgatcacact gatccgcaaa atcaagcagg
    21061 cagataagca gggcgttacc cctaagctgc tggagggtac ttcgctggca atgttgtttg
    21121 aagaaccttc cacccgcacc cgtatttcct ttgaagttgg gatggctgaa ttgggcggac
    21181 atgccctgta cttgaaaccg ggagaaatcc atctgggtgt gcgtgagtca ttgagcgata
    21241 ccgctcacgt catttcccgc tatgtggatg cgatcatggc gcgcacgctg aaacatgaga
    21301 ccatgctgga cttgatgaaa aatgccacgg tacccgtgat caatggattg accgattaca
    21361 accatcccac ccaggtggtc tgtgatgtgc tgaccatgat cgagcatgcc cctgaaggga
    21421 aaaaaatgga agatttcaat gtaacgtttg tgggtgatgc cacgaatgtg ctcagttctc
    21481 tgatgttcat ctgcacgcag ttggggatga attttacgca cgctgctccc aagagatatc
    21541 aggcacccga agcctggatg aagatcgcgc gcgagaactg cgagaaatca ggtggaagcg
    21601 tgcatgtaac ggaagatccg attgaagctg ttaaaaatgc agattttatt tataccgatt
    21661 tgtggtggtg ggttggtcag gaagcagaaa tccccgacag acagaaagcc ttcatgccgc
    21721 gctatcaggt caatatggat ctgatcaaga aggctcccgc tcatgtcaaa ttcatgcact
    21781 gcttgccagc ttcgcgcgga gttgaagcca ccgatgaagt gatggattct gaacattcaa
    21841 ttatttttga tcaggctgaa aatcgcctgc atactgaaaa agggattttg gtgtggttgg
    21901 tctacccgcg cctgaaacat gccagcgccg agcaaaaagc agtccatgct ggtcaaattg
    21961 tagactttct caaaagtcat ttttagcaat cctgttcaaa acaggaaatt caaatggagg
    22021 taaaaatgaa cgcttcaaaa actgtagtaa aagagaaacc aaccggcttc aaaaaagtgc
    22081 tgcgcagcct tgatatgaca cttttcactg tgtgcgccat tctggtgatg gatacgctgg
    22141 cgccttcagc agctattggt ccatcctcaa tttcatggtg gttgatcacc ctggtgcttt
    22201 tctttattcc atacggattg atcaccgctg aattgggaac aacgtatcct gaacagggtg
    22261 ggttgtacat ctgggtgaaa aaagcattcg gcgaaaaatg ggctgcccga accacctggt
    22321 tgtactggat caatgttgcc ttatggatgc catctgttta tattcttttt gcgggcatgt
    22381 tcgcccaatt attcttcccc gaaatggggc tatggtggca gattcttatc ggtatcatta
    22441 tgacctggat caccgtttgg atcgggtcga tcacgctaga aaccagcaaa tgggtcccca
    22501 atatcggtgc cattatcaag gcattgatca tggttgtcat cggagtgggt gcatttattt
    22561 acgctggtaa aaatggcgtc gccaacgatc tttcatttgg taatattctg cctgaatggg
    22621 gcgccggctt ggcattcttg cccgtgatcg tctataactt tatgggcttt gaactgatgt
    22681 caagcgcttc ggaagaaatg aagaaccccg gtaaagatat tccgcgtgcc atcatcattt
    22741 ctggcgcact cattgctgct ttctacctgt tgggcacggt agggatctta atggcattac
    22801 cactggatgg actgggtttg atctctggca tcatcgatac actgagagta ctgctgggag
    22861 agtctggaat tggtggtgca ctggttatgg tgctgggtgt agcagctttg tattccttct
    22921 tggcgaatat ggtcacctgg accatgggtg ccaaccgcac tgcaaatgaa gcggcaaaag
    22981 aaggagaact gccgactgtt tttggcaaag aacaccctgt ttacaaaacc cccgttggcg
    23041 ctttcatcat cactggaatc gtttcaactg cggtcatggt gatttacggt tttatggctg
    23101 gttcggcaga agacctgttc tggacgttat ttgcttttag ctcgatggta tttttgctgc
    23161 cttatctggt catgttccca gcatttttga aattgcgcaa gatcgaccca gacgtggaac
    23221 gcccctacaa ggtgcccggt ggaaaactgt tcataaccat tatgtctgtg atctgtgaaa
    23281 tcttcatcct gcaggcagtg gtgttttttg tttatgtgcc gggtgaaccg atagattggg
    23341 catatgcaac gccagtgttg attggcgtga tcttgaccgt gattgtgggt gaggtattga
    23401 tcgcagtagg taaaaagaaa gcctgatcgc taaatcaaaa aagaaagaag gtccgtgatg
    23461 tcttgtcgaa ttcaaagtac tcctcgcaaa gatggttttc gcatgcccgg tgagtttgaa
    23521 ccgcatacgg gtacctggat gttgtggcct gaacgcccag ataactggcg ggatggagcg
    23581 aaacccgcac agaaggcatt tgcggcgata gcaactgcca tcagtcagtt tgagccggtg
    23641 accatggggg tttcccaatc ccaatttaat aacgctcgtc agatgctgcc ggctgcggtt
    23701 cgcgtggttg aaatggcaaa taatgattca tggatgagag attgcgggcc gacatttgta
    23761 gtgaatggaa ctttggtgcg tgtggtggat tgggatttca atgcctgggg tggactgctc
    23821 ggagggttgt atttcccatg ggacctggac g
//
LOCUS       DAOSDS010000029        23551 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_964_length_23551_cov_4.716888, whole genome shotgun sequence.
ACCESSION   DAOSDS010000029 DAOSDS010000000
VERSION     DAOSDS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 23551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23551
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_964_length_23551_cov_4.716888"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="PLL88_05100"
     CDS             complement(<1..332)
                     /locus_tag="PLL88_05100"
                     /inference="COORDINATES: protein motif:HMM:NF013205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOJ00975.1"
                     /translation="MFVDEAEIHVRSGKGGDGMMHFRKEKFVNRGGPDGGDGGRGGDI
                     VFAVNPNMNTLYRFQHNRTYAAEDGKNGGSTNKTGRSGAALIIEVPPGTIVHERETGR
                     ILADLTEKG"
     gene            complement(397..1716)
                     /locus_tag="PLL88_05105"
     CDS             complement(397..1716)
                     /locus_tag="PLL88_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOJ00976.1"
                     /translation="MRKTRTIIMGAAGRDFHNFNVYFRDNESYEVVAFTATQIPNIEG
                     RKYPQELAGSLYPEGIPIYPESDLKKLIVEQKIDLVIFAYSDVPHEYVMHKASLVNAC
                     GADFRLMGTTATQLKSSKPVISVCAVRTGCGKSQTTRRVSKIMTEMGYKVAAVRHPMP
                     YGDLVKQKVQRFATYADLDKHECTIEEREEYEPHIDNGVLVFAGVDYEAILREAEKEV
                     DVVLWDGGNNDISFYKSDLSIVVADPHRPGHEVTYHPGETNLHLADVFVINKVDTASP
                     ENVITVRENLRAINPEAVIIEGASPLFVDDPDGIRGKRVLVIEDGPTLTHGGMAYGAG
                     WVAAKRFGASEIVDPRPFAVGSILATYQKYPGTGAILPAMGYGNEMVKDLENTVNASD
                     VDMVISATPIDLTRIIEVNKPMQRVRYELQEIGVPTLEDILKKKFKK"
     gene            complement(1745..3505)
                     /gene="argS"
                     /locus_tag="PLL88_05110"
     CDS             complement(1745..3505)
                     /gene="argS"
                     /locus_tag="PLL88_05110"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOJ00977.1"
                     /translation="MFDTYKTPIEEQLTSILEEEGVQTAKFQWSTIPFSGHWGIATSF
                     FQIAAQEARAGKKINVSQRAGELATFCAERLVLPQDFVKCEAVKGYLNLYFQTQSFSR
                     MVIERVLAEGVRYGSLPSTHQKVMVEFSQPNTHKSFHVGHLRNMVLGAAVSNILEFSG
                     DEVIRANYLGDIGLHVITWLWNYEKYHAGEKPPLENKTSWIGNLYAEAVKRREESEEN
                     EEAVRRYFRSWDARDPEIIELWKETRQWSLDAFQEIYNVLGIRFDKLYFESEVEHEGK
                     DFVEGLVQKGLAKDERPDGAVIIDLDAILGTKEEYRVLVLLRSDGTSLYATKDIPLAI
                     KKFEQYHLDRSIYVVDVRQSLYLKQIFKVLELMGYNWAKHCYHLAYEVVNLPGNVTLA
                     SREGTVVLLEDLLREAEKRAMVIVEQKNPELAQTEKQKIARMVALGSIKYSLLSRDNT
                     RIVTFDWESALDVNGQAAPYIQYAAVRANSILRKEKFVVPESEVVLHELDTKEIELVD
                     LLSQFPREVEKASREFKPLQIANYAYDLAKAFNDFYATCPVLSAKDEVRTFRLRLVAA
                     TKQAIINSLGLLGIEVPEIM"
     gene            complement(3583..3849)
                     /gene="rpsT"
                     /locus_tag="PLL88_05115"
     CDS             complement(3583..3849)
                     /gene="rpsT"
                     /locus_tag="PLL88_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOJ00978.1"
                     /translation="MANIKSAIKRNRQNEKRRQKNRVFRGQARTSVRKARAAIESANK
                     EEAQAKVLEAVKALDKAASKGIIHKNNAARRKSRLMKQFVALEK"
     gene            complement(3948..5123)
                     /locus_tag="PLL88_05120"
     CDS             complement(3948..5123)
                     /locus_tag="PLL88_05120"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOJ00979.1"
                     /translation="MFAKKTIKDIDVQGKKVLVRVDFNVPLKDGAVSDDTRIRAALPT
                     IQYLLEGNAAVILCSHLGRPKNGVDPQYSLKPVAEYLARLIPQKVNFAEDCIGPAAQK
                     ASGELKSGEVLVLENTRFHAGETKNDPAMAKELASLADIFVNDAFGSAHRAHASTAGV
                     ADYLPAVAGFLMEKEIQYLGQAIDDPKRPFVAILGGAKVSDKIGVIRNLLKKADMVLI
                     GGGMANTFFKAQGYPVGDSLAEDEALDTARELVKEGANKLRLPVDVVIADKFDDGAEK
                     KTMPVGPVPDGWRILDIGAETVAAYSKIIAGAKTVVWNGPMGVFEFPNFAKGTFEIAK
                     AVADSDAISVIGGGDSVAAVNQTGLVDKISHISTGGGASLEMLEGLELPGLAVLQDK"
     gene            complement(5214..6251)
                     /gene="gap"
                     /locus_tag="PLL88_05125"
     CDS             complement(5214..6251)
                     /gene="gap"
                     /locus_tag="PLL88_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOJ00980.1"
                     /translation="MAVKVGINGFGRIGRQVLKAIKERHGSELEVVAVNDLFSAETNA
                     HLFKYDSNYGAYTGAVTVDGDSIVIDGKPVKVLAEKDPANLKWADLGVDIVIESTGVF
                     TDKLGDPAKGKAGAGVHIEKGGAKKVIISAPAKNEDLTIVLGVNDDMYDPAKHHVLSN
                     ASCTTNCLAPAVKIVHDRWPITRGMMTTIHSYTNDQKILDLAHKDLRRARAAALNIIP
                     TTSGAAKAMALVIPDLKGKFDGMALRVPTPTVSIVDFVATVEKSTSVEELNAAFKEAS
                     EGKLKDIIGFTTEPLVSMDFKGDPRSSIVDGLLTMVMGGTMIKIVTWYDNEWGYSCRT
                     ADLAAKLAKDL"
     gene            complement(6306..7616)
                     /gene="yvcK"
                     /locus_tag="PLL88_05130"
     CDS             complement(6306..7616)
                     /gene="yvcK"
                     /locus_tag="PLL88_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOJ00981.1"
                     /translation="MGKVSGDLQQKFHITLRKILRWLKPGINVKRWLLLMLVGLFCLV
                     SFLTLQINIRNEQSVTSLPFAFSNTPLLRVVLIVVGLLLTSYGFYRFYRSLMRPFIKP
                     GTTLLDTLSSYQKKEKGPRIVTVGGGNGLATLLRGLKQHSHNLTAVVTVADDGGSSGQ
                     LRQSLGILPPGDIRNCLTALSSDEELLTQVFQYRFGEKAGVNGHSLGNLLISALTDIT
                     GSFEEAVAESGRVLAIKGRVLPSTLHDVNLVAEVESREKQEIINVAGESQIPQTGGKI
                     RRLWIEPSNPLPYPPAVQAILAANLIIIGPGSLYTSILPNLLVPGVVEAINSSKALKF
                     FICNVANEHGETDGYRCIDHIRVIEEHLSDIHFDVIICNNNFEPRLPRGVEWVLPDAQ
                     LEEQYSIYYASLVDKEFPWRHDYQKLAEVVVDLLEERTGPLNKS"
     gene            7686..8618
                     /locus_tag="PLL88_05135"
     CDS             7686..8618
                     /locus_tag="PLL88_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOJ00982.1"
                     /translation="MKTILSFDIGGTNIRAGLFSDESGKPSFTKKVKTTTPNRSALEN
                     IFSLIEEILSQYPSLDAISMAVPGSLDNKNGIIIKAPNVDGWKNVALRQIIADKFGIV
                     PFIENDASMAAYGEWKHGAGKNHNYILYITISTGVGGAVIDNGHLLTGFRGLGTEKGH
                     ITIVEDGPPCSCGQKGHLEALASGPAIASYFRQRMVEGASSMLPLDPAPTAKKIAEAA
                     RQGDALAIEAFRQAGYYLGVGIASYLHIFNPSCIILGGGVSFSGNLLYEPFYESLKQH
                     VLSEEYMVGLEIKYAQLGDEAGLIGAYQWSLDNS"
     gene            complement(8770..9141)
                     /locus_tag="PLL88_05140"
     CDS             complement(8770..9141)
                     /locus_tag="PLL88_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00983.1"
                     /translation="MELTGKKPIAIIDASCSKFVSKLKKALKDEGLWVKQSFNLQSIQ
                     NFNDSNLNRSTINRCACNLVIYLVYQNKGDPITLILDERFKKTYIYLSNEDEFAHEAL
                     MLAAISNAIHKVENILRPDEP"
     gene            9675..10112
                     /locus_tag="PLL88_05145"
     CDS             9675..10112
                     /locus_tag="PLL88_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00984.1"
                     /translation="MKKDTKWEDEESALKLSVPLANPKSENLDAIQSALQKAYGITSV
                     KLEKRDSFIDIIYDINQVDFLDIYQSLLKLGLCIPTSKILIEVTKMNCISCIAHLEGE
                     LLDIPGVINVQASISSGTVEMTYVNGLVSGNEIQDAIGRLGYL"
     gene            complement(10174..10845)
                     /locus_tag="PLL88_05150"
     CDS             complement(10174..10845)
                     /locus_tag="PLL88_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ00985.1"
                     /translation="MKNVQKIRIILADDHAIVRAGIRQFLEMEDDFEIVAEADDGGKA
                     KLLLEQFKPDVAILDIQMPEASGIEVTRHIRANRWAIGVLILTAYDDDPYIAAVLKAG
                     ANGYVLKTALPKEICQAVRDVYQGKSFMDKTIVSKVMTYLSSPGGPSIFSNLSDREME
                     VLTLAAKGKTNRAIAHQLSISDRTVQGHFARIFEKLQATSRTEAVMKAVAMGLISPDI
                     AHSNE"
     gene            complement(10928..12253)
                     /locus_tag="PLL88_05155"
     CDS             complement(10928..12253)
                     /locus_tag="PLL88_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ00986.1"
                     /translation="MRSSEAGTAILFPNHPINGDLYTPIASHFDPGMVVIGLFSPEKI
                     LSTGLSILLTENHANFRVLDEQNNVLFQTNQGSNSESVLDYQELQNILDEKNGIDFLK
                     TSKGEVVITAAKIETVGWILVDEESWQDIASPLLRITQNAPLIIVPLLGFSILVLWFG
                     FRQIVQPLQELGSKAGDVAKGDFKSIKEPVGGVPEIVHLQNSMMGMAEKLKHAKNNLQ
                     NYLGAITDGIENERRNVARELHDDTLQSLIALGQNTQYAKHWNKDPKVEKSLDQISHL
                     TDQGIVNLRRLVQGLRPIYIEDLGLTTALSMLASGYPNPTGIKVHFHQNGNECRFSSN
                     VEMTLYRITQEALSNAFRHASAQNAWISIDFRERDVILEVRDDGIGFEVPQESSHYAS
                     GGHFGLLGMYERSELIGAKLNVQSVKGEGTRVTVQYFDSSSKKRLENIG"
     gene            12296..12586
                     /locus_tag="PLL88_05160"
     CDS             12296..12586
                     /locus_tag="PLL88_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00987.1"
                     /translation="MNRRLIKIKSWRAVRVNQCKTAIKDGQFVLKLSDGRLKILAAFC
                     ESGNQFDGFRPMVNISIQADRCRLNCMQITLCDQRSHCLMMQSQAAKGNDEH"
     gene            complement(12732..13952)
                     /locus_tag="PLL88_05165"
     CDS             complement(12732..13952)
                     /locus_tag="PLL88_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOJ00988.1"
                     /translation="MKRLLPIAIILIASLLKPLPVFANDISDQPSVEIFFSAGCSDCW
                     PYTQDVLIPALRSGGISAQYQVHDFTIPEERHRLSDMIDDIGLPRSIADSLYAFIPTS
                     EGMLVVLGHVPAQLIEAALQSPDLPQKLVIWQPKMHGDPTEYKLWAWKGEVQTFPIET
                     SFDEALTATINASGVLPVGLANLSELLPAVLVTGLVDSVNPCAFAVILLLIAFLFTLR
                     QSRARILQLGFAYIAMIFVVYFSIGLGLLRVIRLSDDPHFIARVGSWLLIFLGSINLI
                     EFFFPKFPFKLHMPAFVGDRTYELIKKATLPATLVAGFLVGLCTFPCSGGIYVSIITL
                     LNAKTTQSWGLVYLLLYNLMFVLPLVIILLALGNRLVAKKWAGWERQHSLKIRLWYGL
                     VMVAMGIIMLILVI"
     gene            14270..14758
                     /locus_tag="PLL88_05170"
     CDS             14270..14758
                     /locus_tag="PLL88_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00989.1"
                     /translation="MKSKRKQSKFYKNKKSFPIWGSVIIALILLFVGVSFLNTVLTPR
                     EKIEVTGSPKIKVDQDYYDYGNVKNGGVPIRTSIKVSNIGDKPLNFQEAPYIELIEGC
                     CPPVPSIGTLSLAPGESTQINVEFFMHGTMGGMHNFKLHLVTNDPAEPDKSITIISNW
                     VD"
     gene            15000..15824
                     /locus_tag="PLL88_05175"
     CDS             15000..15824
                     /locus_tag="PLL88_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00990.1"
                     /translation="MAKQTDSAKISGTTEIDVISRSTFPQDGYRIPVILGNIGSQLIE
                     MGVINRTQFAQAHAEMGRPLSAEQMKVFDGTYAGKVFINEENSYFLLNFFWALGLANK
                     NPILDSGLIQKYSNGEIEMFASTGGWTFASHPINEIFSSQEIIPLNDAQQKIVEEAAS
                     RIYRPCCNNPSDFPDCNHGMAMLGLLELMGSQDKNLDEMLEAAKYVAAYWFQEQTLAQ
                     AVYFKKAENKEFKDVDANRILSSLYSSASGFNELYTFILEKKWMPSSNIHLSNCGG"
     gene            16030..17343
                     /locus_tag="PLL88_05180"
     CDS             16030..17343
                     /locus_tag="PLL88_05180"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HOJ00991.1"
                     /translation="MHINPGPISEGLIERDKKVISSSYPRGYPFVMDHGKGVEVWDVD
                     GKRYLDFAAGIAVNSTGHSHPEVVAAITEQAQKFIHISSDFYHQKWIELAEKLDEISP
                     FHLPAISFMANSGSEAVESALKLAKYHTERSQFIGFLGGFHGRTTGSLSMTASKLIYH
                     RSFYPLMGGVTHVPYPNPYHPILHMEEGEDAGDAVIRYLEEIVFHKLLPPTEVAGILI
                     EPVQGEGGYVFPPNDFLPALREICDKYGILLIADEVQSGMGRTGKWWGVDHYGVEPDI
                     ICIAKGIASGMPMGVMLAKAELMTWPNGSHGNTFGGNPLACAAALKTIELIENQYLAN
                     TQKMGTIALEFLQQLQTGHACIGDVRGKGLMIGVEFVEDRKTKQPAVDLRNDVVDLCF
                     QKGLLTLGCGDSTIRITPPLCINEQEVREGLEIFAAAVKEVESHL"
     gene            17377..18930
                     /locus_tag="PLL88_05185"
     CDS             17377..18930
                     /locus_tag="PLL88_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ00992.1"
                     /translation="MFPDFRVRQRDYLLEISQALTQELDLDVLLNKILKYAIEMLSGH
                     AGLIALRSEESGWHIRVSEGISPDFLEHMKPFLKQIPDDQDPEKFEVPEVNRVLSEIT
                     FSATLGLLSGVGLPLITQQTVVGLIFIFRSYAGLFTANDRILLRSFATQAAIAVQNAQ
                     LYRSVIRNKQRLDALFDSAADGILLLTSNQVIEHCNPAFTRLAAQPSEEIIGKPHDQI
                     VRLENTTQEQTLEKAIAGGWPLTPHAQLYVEGDLVRPGLPSLPIGITYAPMLSPENKL
                     TNIIATFRDITNFRKADEAKTTFISIVSHELKTPVALIKGYVCTLNRKDVEWDSAFVD
                     ESLKIIEEEADHLNELIENLLDATRLQTGKFSIQKTDISIPDIAQRLVERFSTQTDKH
                     TFKIDFPKDFPSVLADDTRIEQVLSNLISNAIKYSPKGEVTISGETRRDKVIVCVSDE
                     GSGVSQQDLPFIFDRFYRSPDAARKTKGTGLGLYLTRQIIEAHKGRIWIDTERDKGAC
                     FCFSLPRNE"
     gene            18979..20331
                     /locus_tag="PLL88_05190"
     CDS             18979..20331
                     /locus_tag="PLL88_05190"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HOJ00993.1"
                     /translation="MPKTQVACPQSRQPILVEVQQIFDLAQDPLAKQKLLNGAVNVIH
                     CPACGYSGMLGTPIVYHDPEKELFLTFFPPESGVPLNEQERQLGPMINRVIDALPAEK
                     RKAYILQPKSMLTYQTLIESVLEADGITKEMLEEQQKKIHLIQRLLTAPETELETIIE
                     QEKELIDLSFFALYSRLLQSAVAQNDEESARQLDKVQKVLFEKTEVGKELYKSSQETQ
                     QAIKELQEAGKDGGLTREKLLDVVLNAKSDAALSAIVSLARNGMDYTFFQLLSQKIDA
                     AKDKEKESLVTLRENLLNLTSELDKRMQAEVDKTKELLESIVKAENIEAEAEKNLENI
                     TELFLHVVEDEIEKARKQGDLERIQKLERVMIVIRKAMEPPQEVKLIEEMLALVDKQD
                     ELEKFMDAHQESITPELVQLLNGILSQAGNAQDEGYQKFDQLYKAVLRYSMKKNLAAK
                     "
     gene            complement(20377..21024)
                     /locus_tag="PLL88_05195"
     CDS             complement(20377..21024)
                     /locus_tag="PLL88_05195"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOJ00994.1"
                     /translation="MEKKNTSLLIAQNGKLNGKQWLLDDEIVIGRDAECELVIDDRQV
                     SRKHARILQTPEGIFVEDLNSKNGTYINHRKVEGTAQVFDGDFIQIALIQEFLFVASD
                     ATMPLSEEQAYRIVFIDKGNGAVWVNDKKIDPPLSIQQYTLLLCLYEQSGNIVPRSEI
                     IARVWADSAGAGISNEAIDALVRRLRYRLTEYEPDVELIETVRGYGFRFQNPIKR"
     gene            21082..22065
                     /locus_tag="PLL88_05200"
     CDS             21082..22065
                     /locus_tag="PLL88_05200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOJ00995.1"
                     /translation="MAISRKPGEVLQNRYLIKKIIGRGGMGSVYLADDTLLEGRQCAL
                     KEVEYDRSLDSVLIKEAQEQFLREATILARLDHPNLPKVSDFFTIESCDYLVMDYVPG
                     EDLRTIILQAKQNNSFLPLSDIRNWADQLGDALSFLHSQEPPIVHRDIKPSNLKITPS
                     GILKLVDFGLVKILVPDDVTITVIQGQGTAIYTPLEQYGGDGIHTDVRSDIYSFGATL
                     YHLTTNTQPADARERFLQPGRLMPPGEINPAISSQMEKAILWALQLHPDDRPQTVKQF
                     TDALLGNTKKLVEKINPHIDHRRLSSNKNVKYLFWISVGLLFLSFLISFFA"
     gene            complement(22006..>23551)
                     /locus_tag="PLL88_05205"
     CDS             complement(22006..>23551)
                     /locus_tag="PLL88_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00996.1"
                     /translation="VFSLSFVLPAEQQLGIIGQGYDVLSKIARNAATLINPSSEELSS
                     ILPDPPTTNDRIVFITDTMSAAQCSTCEEEEIFTKNALEDTVLRLYGQNGTGQVLERR
                     LRSYSFEDLSEYLDDPTDRADVGNYISEANWVIFSMLDVNADRPGSDALKRLLAENPD
                     MLRDKNVVVFAFNVPFYLDATDLTFVDAYYCLYSKTSVFIDTAARLLFQEIVPKGHSP
                     VSIPVVGYDLIEMTYPDPNQTITLDIDVNNLSESKRNESETAGTENLIFRVGDALPVR
                     AGPILDRNGLPVPDGTVVYFMIDHLGENEQSTIIETQTKSGFASMSYPIQSYGRVEIK
                     AQSDPARNSDIFILDITSEAGSVISQITPTPVPTQPSASSVVNQGGFGDQTMTVENGG
                     RKSLPGTMQWFLATLTILGIAYGIYLLTQRFFSVTEGTIIALCAVIAGYGCFLWFQLN
                     LPGSFSRTNFLELVYLLLWVSLFTLLGAGFGMGQQSKIWKKFLTASGKEADQKTEKKQ
                     PNGNPK"
BASE COUNT         6213 a         5871 c         5319 g         6148 t
ORIGIN      
        1 cctttttccg tcaagtcagc caggatgcgc cccgtttctc gctcatgaac gatcgtgccg
       61 ggtggcactt cgatgattag cgctgcaccc gatctgccgg ttttgttggt tgaaccacca
      121 tttttcccat cctcggctgc ataggtgcgg ttgtgttgga accggtagag cgtgttcatg
      181 tttggattaa ctgcgaacac aatatccccc ccgcgaccac catctcctcc atctggaccg
      241 ccccggttga caaatttttc cttgcggaaa tgcatcatcc catctccccc tttaccggaa
      301 cgcacatgga tctctgcttc gtctacgaac atgctgttat tataatcaaa aagaggaggt
      361 caactctctt ggacctcctc ttttggtatt cggatttcac tttttaaatt ttttcttcaa
      421 gatgtcttcc agggtaggaa caccgatctc ttgcagttca tagcgcacgc gttgcattgg
      481 tttgttcacc tcgatgatcc tggtcaggtc gattggcgta gctgagatga ccatatccac
      541 atccgaggca ttgacagtat tctcaaggtc cttgaccatt tcatttccat agcccatggc
      601 tggcaagatg gcgcccgtgc cggggtattt ctgataagtt gccagaatgg aacctactgc
      661 gaagggtcgt gggtcaacga tctcactggc tccaaaacgc ttggcagcca cccatcccgc
      721 cccatacgcc ataccgccgt gcgtgagggt cggaccatct tcgatgacca gcacacgttt
      781 ccctctgatc ccgtctggat catccacaaa caagggtgaa gcaccttcga tgatcaccgc
      841 ttcaggattg atggcgcgca ggttctcgcg cactgtgatg acgttttcag gacttgccgt
      901 gtcgacctta ttgatcacaa aaacatccgc caagtgcagg ttcgtctcgc cgggatgata
      961 cgtaacctca tgccctggtc ggtgcgggtc agccacaacg atcgaaaggt cagatttgta
     1021 aaacgagatg tcattgttcc cgccatccca caacactaca tccacttctt tctcggcttc
     1081 gcgcaagatc gcttcgtaat ccacaccggc gaagaccagc acgccgttat cgatatgagg
     1141 ctcatattcc tcccgttcct cgattgtaca ttcatgttta tcgaggtcag catacgtagc
     1201 aaagcgctgc accttttgtt tcaccagatc gccatagggc atgggatgtc ggacggctgc
     1261 aactttatat cccatttcgg tcatgatctt ggaaacccta cgggtagttt ggctcttccc
     1321 gcagccagtg cgaaccgcac aaacggagat gaccggtttg gaggatttca attgtgtggc
     1381 agtcgttccc atcaacctga aatccgcacc acatgcattt acaagagatg ccttgtgcat
     1441 gacatattca tggggaacat cactgtaggc aaatatcacc agatcaattt tctgttcaac
     1501 gatcagcttt ttcagatcgc tctcagggta gatcgggatc ccttcggggt ataacgatcc
     1561 tgccaattcc tggggatact tgcgcccttc aatgttcgga atttgggtcg cagtaaaagc
     1621 gaccacttca taactttcat tatcgcgaaa atagacatta aaattgtgga agtctcttcc
     1681 cgctgctccc atgatgatgg ttcgtgtttt tcgcatgcaa ctctcctttg ttacgccatt
     1741 gttattacat gatttcaggg acttcgattc ccagcaagcc cagactgtta ataatagcct
     1801 gttttgttgc tgccacaagt cttaatcgga aggtacgaac ctcatctttc gcagaaagca
     1861 ccgggcaggt ggcatagaaa tcattgaatg cctttgccag gtcataagcg taattagcga
     1921 tttgtaacgg cttgaattcg cggctggctt tttcaacttc gcgcggaaac tgtgaaagca
     1981 aatctaccag ctctatttct ttggtatcca attcgtgcaa cacaacctca gattctggaa
     2041 caacgaattt ttcttttctt aaaatgctgt tggcgcgcac cgctgcatac tggatgtacg
     2101 gagctgcctg cccattaaca tccagagcgc tttcccaatc gaacgtgacg atgcgggtat
     2161 tatcgcgcga cagcagcgaa tacttaatgg agcccaatgc caccattctg gcgatctttt
     2221 gtttttccgt ttgagcaagc tcggggtttt tttgctccac aatgaccata gcgcgttttt
     2281 cagcctcgcg cagcaggtcc tcaagcagca ctacggttcc ctcgcgcgaa gccagggtta
     2341 catttcccgg caggttcacc acctcatacg ccaggtgata gcagtgcttc gcccagttgt
     2401 accccatcaa ttccaacacc ttgaatatct gcttcagata cagggattgt cttacatcta
     2461 ccacgtaaat ggaccgatcc agatggtact gttcgaactt tttgatcgca aggggaatgt
     2521 ctttggtggc atacagcgac gtgccgtctg aacgcaacaa aaccaacacg cggtattctt
     2581 ctttcgtccc caaaattgcg tccagatcga tgatcacagc tccatccggg cgctcatctt
     2641 ttgccagccc tttctgtaca agcccttcta caaagtcttt cccttcatgc tccacctcgc
     2701 tctcaaaata gagcttgtcg aaacggatac ccaggacatt atagatctct tgaaaagcgt
     2761 ccaatgacca ctgacgggtt tctttccaca attcaatgat ctccgggtca cgcgcgtccc
     2821 acgacctgaa atagcggcgc acggcttctt cattttcctc tgactcttcc cgccgcttaa
     2881 cagcctcagc gtacaggttt ccgatccaac tggttttgtt ttccaacgga ggtttttctc
     2941 cagcatggta tttttcataa ttccacaacc aggtgatgac atgcaatccg atatccccca
     3001 gatagttcgc acggatcact tcatcaccgc tgaactccag aatattggaa actgccgctc
     3061 ccagaaccat gtttcgtaga tgacccacgt gaaaagactt atgcgtattt ggctgcgaga
     3121 attccaccat caccttttga tgggtagatg gcaaactgcc atatctcacc ccttcagcca
     3181 gcacccgctc aatgaccatg cgcgagaaac tctgcgtttg aaaatacagg tttaaatagc
     3241 ccttaactgc ttcacatttc acaaaatcct ggggcaaaac caaccgttcg gcacaaaacg
     3301 ttgccagttc gcccgcccgt tgcgatacat tgatcttctt ccccgcccgg gcttcttgcg
     3361 ctgcaatttg aaagaaagag gtcgcgatgc cccaatgccc gctgaaaggg atggtgctcc
     3421 actgaaactt ggcagtctga accccctcct cctccaaaat ggatgtgagc tgttcttcga
     3481 ttggcgtttt atatgtatca aacataagac cccataaaat gaaaagcggg agaactgccc
     3541 tcccgcattc aactcgaaga aatagaaaag gaatgaatca gattatttct cgagggcaac
     3601 gaactgtttc atcaatctgc ttttgcgacg ggcagcgtta ttcttatgaa tgatcccttt
     3661 gctggctgcc ttgtctaaag cttttaccgc ctccaatact ttggcttgcg cttcttcctt
     3721 gtttgcggat tcgatagctg cacgtgcttt tctaaccgag gtgcgtgcct gccctcgaaa
     3781 aacgcgattc ttctgtcgcc gcttttcatt ctgcctgttt cgcttaatag cggatttgat
     3841 atttgccaaa ataacctcca aaaatttcac ttgtgcaagc caagatttta ctagacagta
     3901 ccgcttttgt ctagttgaaa gaggagctcg caggagctcc tctttggtta cttatcttgt
     3961 aacactgcca atcccggcag ttccaagccc tccaacattt ccagagatgc cccaccaccg
     4021 gtagaaatgt gcgatatctt atcaaccaga ccggtctgat tcactgccgc tacagagtcg
     4081 ccgccaccga tcacgctgat cgcatcgctg tcagccaccg ctttggcaat ttcgaaggtt
     4141 ccctttgcaa agttgggaaa ttcgaacacc cccataggac cgttccatac cacagttttt
     4201 gcgccggcga tgatcttgga ataggcagcc acagtttctg caccaatatc gaggatgcgc
     4261 cacccatccg gaacaggtcc aacgggcatg gtcttctttt ctgcaccatc atcgaatttg
     4321 tctgcaatca ccacatctac cggcaggcgt agtttattcg cgccttcctt gaccagttcg
     4381 cgggcggtgt caagcgcttc atcttctgcc agtgaatctc caaccggata cccttgcgct
     4441 ttaaagaagg tatttgccat tccgccgccg atcaaaacca tatcggcttt tttcaacaga
     4501 ttgcggatga cgccgatctt atcgctcact ttggcaccac ccagaattgc cacgaacggt
     4561 cgctttgggt cgtcgatcgc ctgaccgagg tactggatct ctttttccat caagaatccc
     4621 gccaccgccg gcaggtaatc tgccacgcct gccgtagagg catgggcgcg gtgggctgag
     4681 ccgaaggcat cgttgacgaa gatatcagcc aaactagcca gctctttcgc catggctgga
     4741 tcattctttg tttcaccggc gtgaaagcgg gtattctcca gaaccagcac ttctccgctt
     4801 ttcagctctc cagatgcttt ctgtgccgct ggtccaatgc aatcttcggc aaaattcacc
     4861 ttttgcggga tcagcctggc aaggtattcc gccaccggtt tcaggctgta ttgtgggtca
     4921 acgccatttt tcggtctgcc aaggtgcgag cacagaatga ccgctgcgtt cccctccagc
     4981 aaatattgga tggtcggcag cgctgcgcgg atgcgggtat catcactgac cgcaccatcc
     5041 ttcaaaggca cgttgaagtc aacacgaacc aacacttttt tgccctgaac atcgatgtct
     5101 ttgattgtct tttttgcgaa catgtttcct ctcatttcaa ggtatgaaaa aggcataatc
     5161 gcgctttcat ggtctgatta tgcctttcat tctacatgag gtcctatgaa aaattatagg
     5221 tcctttgcca actttgccgc caggtcagcg gtacggcatg aataacccca ttcattatcg
     5281 taccaggtta cgatcttgat catcgtgccg cccatcacca tggtcagcag accatcgacg
     5341 atggaagagc gtggatcgcc cttgaaatcc attgaaacga gcggttcggt ggtgaagcca
     5401 ataatgtcct tcaattttcc ttccgaagct tccttgaaag cagcattcag ttcttccaca
     5461 gaggtcgatt tttcaacggt tgccacaaaa tcgacgatgg atacggttgg ggtaggaacg
     5521 cgcaatgcca ttccatcgaa tttccctttc agatcaggga tgaccagtgc catggctttt
     5581 gccgcaccgc ttgtggtcgg gatgatattc aacgctgctg cgcgagctct gcgcagatct
     5641 ttgtgagcaa ggtcgaggat cttctgatca ttggtgtatg agtgaatggt ggtcatcata
     5701 ccgcgggtga tgggccatcg atcatggacg atcttcactg cgggtgccag acagttagtg
     5761 gtgcaggatg cgttggacag cacgtgatgt ttcgcaggat cgtacatatc atcattcacg
     5821 cccaaaacga tcgtgagatc ttcgtttttt gcgggtgccg aaatgatgac tttctttgcg
     5881 ccaccctttt caatgtgaac gccggctccg gctttgccct tggcgggatc gcccagttta
     5941 tcggtgaaca cgccggtcga ttcaatgacg atgtcaacgc ccaggtctgc ccatttaaga
     6001 ttcgcagggt ctttttcagc caaaactttt acgggtttcc catcgattac aatcgaatct
     6061 ccatccacag taacagcacc ggtataagca ccatagtttg aatcatattt aaataaatgc
     6121 gcgttggtct cagcgctgaa caaatcattt actgcaacaa cttccagctc gctgccatgt
     6181 cgttctttga ttgcttttag aacctgacga ccaatgcggc cgaatccatt aataccaact
     6241 ttaactgcca tgatcgttat cctccggatt tttttgtatt ttaccacgcc ttttttaaac
     6301 gtggtctatg atttgtttaa gggaccggtt cgctcttcaa gcaggtccac cacaacctca
     6361 gccagttttt gataatcatg ccgccatgga aattctttat ccaccagcga tgcataataa
     6421 atcgaatatt gctcttcaag ctgtgcgtca ggtagcaccc attccacgcc cctcggcagc
     6481 ctgggctcaa aattattgtt acagatgatc acgtcaaaat ggatatcgct cagatgctct
     6541 tcaatcacgc gaatgtgatc aatacagcga tacccatccg tttctccatg ttcattcgca
     6601 acgttgcaga taaagaattt caaagctttg ctcgaattga tggcttccac aacccccggc
     6661 accagcagat tgggtagaat gcttgtgtaa aggctgcccg gaccaatgat gatcaagttg
     6721 gcagccaaaa tcgcctgtac ggcaggaggg tatggcaagg ggttgcttgg ctcgatccat
     6781 aatcgtctga tcttcccccc tgtttgcggt atctggcttt ccccggcgac attgatgatc
     6841 tcctgttttt ccctcgactc tacttcggct accagattca cgtcgtgcag tgtggatggc
     6901 aacaccctcc ccttgatcgc caatacccgc cccgattctg ccaccgcttc ttcaaaactg
     6961 ccagtgatat cggtgagtgc gctgatcaag agattcccta acgaatgccc gttcacgccc
     7021 gccttctcac cgaagcgata ttgaaagacc tgcgtgagca gttcttcatc gctcgataat
     7081 gcggtcaggc agttacgaat gtcgcccggt ggcaatatgc ccagactttg cctcaattgt
     7141 cctgacgatc ccccgtcatc cgcaaccgtc acgactgcgg tcaggttgtg tgagtgctgt
     7201 ttcaaaccgc gcagcaaggt agccagccca tttccccctc ccacagtaac gatgcgcggt
     7261 cctttctcct tcttttggta actcgaaaga gtatccagca aagtcgtccc cggcttgatg
     7321 aaagggcgca tgagtgagcg gtagaatcga taaaaaccat aagaagtaag caaaagccct
     7381 accacaatca gaaccactcg tagcaagggg gtgttggaaa acgcgaatgg caatgaggta
     7441 acgctttgct cattcctgat gttgatctgc aaggtcagaa aggataccaa acagaataat
     7501 cccaccagca tcagcagcag ccagcgtttg acgttgatcc ctggtttcag ccagcgtaag
     7561 atcttacgga gggtaatgtg aaatttttgt tgcaggtcgc cagaaacttt gcccatcagt
     7621 cagcattata gcaggatttt tcgcctcgtt tctctatcct tcagaaaatt agtataatca
     7681 tatctatgaa aacaattctt tcattcgaca ttggcgggac aaacattcgc gcggggttgt
     7741 tttctgatga atcaggcaaa ccgtctttta caaaaaaggt caagaccacc actccaaaca
     7801 gatccgctct ggagaatatc ttctcactga tcgaagagat cttgtctcaa tatcccagcc
     7861 tggatgccat cagcatggcg gtccctggca gtcttgataa caagaatggc atcattatta
     7921 aagcccccaa cgtggatggc tggaagaatg ttgcgttgcg ccagatcatt gccgataagt
     7981 tcggcattgt acctttcatt gaaaacgatg ccagcatggc agcatacgga gaatggaaac
     8041 acggcgcagg aaaaaaccat aattacattc tctatatcac catcagcacc ggtgtggggg
     8101 gcgcagtaat cgataatgga catcttttga ccggctttcg tggtttaggc accgaaaaag
     8161 gacatatcac gatcgtggaa gatggtccgc catgttcttg cggtcaaaaa ggacacctcg
     8221 aagcactggc atccggtcca gccatcgcct cctatttccg tcagagaatg gtcgagggcg
     8281 cttccagcat gctgcctctc gatccagccc ccaccgccaa aaagatcgcc gaagctgcca
     8341 ggcagggtga cgctctggct attgaagctt ttcgtcaggc agggtattat ctgggcgtag
     8401 ggattgccag ctatctgcac attttcaatc cttcctgcat catcctgggc ggaggcgtat
     8461 ccttcagcgg caacctgctg tacgaaccat tctatgaatc actgaaacag cacgttctca
     8521 gcgaggagta catggtaggg ctagagatca agtatgccca gcttggtgac gaagccggct
     8581 tgatcggagc ataccagtgg agcctggata actcttaagg cagatggttc ttgctcatta
     8641 actcgagtat cccatcgcag gaatgatggg gggagggaaa aatcgcttta attcatcagg
     8701 atccaacccc catgctttaa ttttcttgaa ctcttttctc aatgagaaag tttgttgatc
     8761 cgaaagaaat catggctcat caggacggag gatgttttcc accttatgaa ttgcattcga
     8821 aatggctgcg agcatgagcg cttcatgggc aaattcatct tcattgctca aataaatata
     8881 ggtcttcttg aaccgttcgt ccaggattaa ggtgatagga tcgcctttat tttgataaac
     8941 cagataaatc accagattgc aggcacaacg attgatagtg ctccggttca agttgctatc
     9001 gttaaaattt tgaatgctct gcaagttgaa tgattgctta acccacagac cctcatcctt
     9061 caatgctttc ttcaatttcg agacaaattt cgaacatgaa gcgtctatga ttgctatggg
     9121 ttttttccca gttaattcca tgccggttcc aataacttcg tttcaataag tcttcaagaa
     9181 aatttcatct ttctatgaag aaaggaaatt caacggatgg agtgaggctt agaaaggtta
     9241 agcattgtta ttgcaaatgg atcatgattg attggtgttc tcatggtcat gatgattttc
     9301 tccggaatta tggctcatta aacgcatcat caaaatgtgg gaaagaggac aagccaggag
     9361 caaaatgaac aataatccgt ttcggagggg aatcttcaac aggagaacag ccgctattac
     9421 cccaacgaca accagaacac aaatcttcat taaattttga tgtttgtcca gcattcttac
     9481 ctcaactcgt tgaatttcac catactatat ccaagccttt tttctgtcat gcgtcatttt
     9541 gcttaaagtc gctcgcgtat ttttgctcga accattaaat ttcatcgaat aagcagaaaa
     9601 acctgttttg cataagcaat tttgcgctta tgaattactg atgcatcgat atactgaaaa
     9661 tgcatggagg ctaaatgaaa aaagatacga aatgggaaga tgaagaatcc gcattgaaac
     9721 tcagcgtacc tctggctaat ccaaagagtg aaaatttaga cgccatacaa agcgcattgc
     9781 aaaaagcata tggaataacc tcggtgaaat tagaaaaaag ggactcattc attgacatca
     9841 tatacgatat caatcaggtg gattttttgg atatttacca atcgttgttg aaactgggcc
     9901 tgtgcatccc aaccagcaaa attcttattg aagtgacgaa gatgaactgt ataagttgca
     9961 ttgcccatct ggaaggcgaa ctgttggata tccccggagt aatcaacgtt caagccagca
    10021 tttccagtgg gactgtggaa atgacgtacg tcaatggact ggtatcaggg aatgaaatac
    10081 aagatgcgat tggtagattg ggatatctct aagagaaaat gattgcggtc ttgccgatca
    10141 tgaaaaccaa aattgattcc atgatatctg ttgctattca ttcgaatgag cgatgtctgg
    10201 agatatcaag cccattgcta ctgctttcat gacagcctca gttcggctgg tagcctgcaa
    10261 tttttcaaat atgcgggcaa agtgaccctg aacagttcga tcgctgatgg ataattggtg
    10321 ggcaattgcc cggttagttt ttccttttgc tgccagggtt aatacttcca tctcacgatc
    10381 ggaaagattg gagaatatcg agggaccacc aggggatgat aagtatgtca tcacttttga
    10441 aacaattgtt ttatccataa aggatttccc ttgatacaca tcccgcacag cctggcatat
    10501 ttcttttgga agggctgttt ttaatacata gccattagct ccagctttaa gaacggcagc
    10561 tatgtaagga tcatcatcat aagctgttaa tatcaaaacg ccaatcgccc agcggttagc
    10621 tcgaatgtgt cgtgttacct ctattccgga tgcctccggc atttgaatat ccagtattgc
    10681 cacatcaggc ttgaactgct ctaaaagcaa cttggctttt ccaccatcat cggcttcagc
    10741 cacgatttca aaatcatctt ccatttccaa aaattggcgt atgcctgccc gaacaattgc
    10801 gtggtcatca gccagaatga tgcgaatttt ttggacattt ttcatgaggt ttttcctgtt
    10861 gaactattgc agttaaaaat atgaatcatt gggtgatact tttttgccaa gattgccatc
    10921 ttgctcatta tcctatgttt tctaatcttt tttttgaact cgaatcaaaa tattgaacgg
    10981 tcacccgggt tccttcgccc ttgaccgatt ggacgttgag tttggctccg atcaattcgc
    11041 tccgttcata catgcccaat agaccaaagt gaccaccaga tgcgtaatga gatgactctt
    11101 gaggaacttc aaaaccaatc ccatcatcgc gaacctcgag aatcacatct cgttcacgaa
    11161 aatctattga gatccatgca ttttgggctg aagcgtgacg aaatgcattg ctcaacgctt
    11221 cttgtgtgat gcggtacaaa gtcatctcca catttgagct aaatctgcac tcattcccat
    11281 tttggtgaaa atgaactttt atgccggtag gattcggata gccggaagcg agcatagata
    11341 aagcggtggt caagcccaag tcttcaatat agatcggtct tagaccctgc acaagtctgc
    11401 gaagattaac aattccttga tctgtcaaat gactgatctg gtcaagcgat ttttcaacct
    11461 tgggatcttt attccaatgt ttcgcatact gcgtattttg tccgagggca attaaagatt
    11521 gcaaggtgtc atcatgtaat tcgcgtgcca cattgcgacg ctcattttca atgccgtctg
    11581 ttatagcgcc tagataattt tgtaaattgt ttttggcatg cttaagcttt tctgccattc
    11641 ccatcatgga attttgaaga tgaacaattt ctggaacgcc gccaaccggt tcctttatgg
    11701 atttaaagtc accttttgcc acatcacctg ctttagagcc caactcctgc aggggttgaa
    11761 cgatctgtcg aaaaccaaac cataaaacca gaatcgaaaa cccgagaagg gggacgataa
    11821 ttaaaggtgc gttctgtgta atccttaaga gtggacttgc aatgtcttgc cacgattctt
    11881 catctaccaa aatccaacct accgtttcaa tctttgcggc tgtgatgaca acttcgcctt
    11941 tggaagtctt taaaaagtca atcccatttt tctcatccaa aatattctgt aattcctggt
    12001 agtcaagaac actttcacta ttgctgccct gattcgtttg aaaaagaacg ttgttttgtt
    12061 catccaagac tcgaaagttt gcatgatttt ccgttaacag gattgataat ccagtggata
    12121 aaattttttc cggtgaaaat aacccaatga caaccatacc agggtcgaag tgtgaagcga
    12181 tgggtgtgta caggtctcca tttattggat gattgggaaa aagaatagcg gtgcccgctt
    12241 cagaggagcg catagaactg acaatctttt gccaatcgaa agatgaggaa aattgttgaa
    12301 caggagattg atcaaaataa aaagttggcg tgccgttcgc gtcaatcaat gcaagaccgc
    12361 catcaaagat ggacaattcg tcttgaaact gagcgatggt agattgaaaa ttctcgctgc
    12421 cttctgcgaa tcgggaaatc agttcgatgg ttttcgtccg atggtcaata tctcgataca
    12481 ggctgatcgc tgcagattga attgcatgca aatcacgctc tgcgaccagc gatcgcattg
    12541 cctcatgatg caaagccagg ctgccaaagg taatgatgag cactaacaaa gacaagggca
    12601 aaacagcaat caagaacaac tgcatggtaa cccctcgaaa accatggctg gttcttttcc
    12661 tgttcgcctt ctttttggca tttttcaaat ttgtatgcct cacagaacat tgggtttcaa
    12721 tatttactat tttatatcac caaaattagc atgatgatcc ccatggcaac catgacgaga
    12781 ccgtaccaaa gacggatttt caatgagtgt tgcctttccc aaccagccca cttcttggct
    12841 accaatcgat ttccaagagc aagaaggatg atgaccaggg gaagcacaaa cattaaattg
    12901 tacagtaaca gataaaccag tccccacgat tgagtcgttt ttgcattaag taacgtgatg
    12961 attgatacgt aaatgccccc cgagcaaggg aaggtgcata aaccaaccaa gaaacctgcc
    13021 accagggtag cagggagggt cgcttttttt atcaattcat aggtgcgatc tccaacaaag
    13081 gcgggcatat gtaatttaaa gggaaacttt ggaaaaaaga actcaatcag attgatgctc
    13141 ccgaggaaaa tgagcaacca tgaacccacc cgggctatga agtgtggatc atccgatagc
    13201 cttattacgc gcaataatcc caacccgatg ctgaaataca caacgaatat catcgctata
    13261 taggcaaagc ctaactgaag tattctcgcg cgagactggc gtaaagtaaa cagaaaggcg
    13321 atcagaagca aaattacagc gaacgcacaa ggattaaccg aatcaaccaa ccctgttacc
    13381 aacactgccg gcaataattc cgaaaggttc gccaatccga cgggtaggac acccgatgca
    13441 ttgattgtcg ccgtcaaagc ttcatcaaat gatgtttcaa taggaaaggt ttgaacctcc
    13501 cctttccatg cccaaagctt atattccgtt gggtcaccat gcattttagg ttgccaaata
    13561 accagttttt gtggaagatc tggagactgg agggcagctt cgatcaactg cgcagggaca
    13621 tgacccaata caaccagcat gccctcgctt gtgggaatga aagcatacaa actatcagca
    13681 atggaacggg gcaatccaat gtcatcaatc atgtcagaca aacggtgccg ttcttccgga
    13741 atcgtaaaat cgtggacttg atattgagcc gaaattccgc cagatcgcag cgccggtatc
    13801 agcacatctt gtgtataggg ccagcaatca ctacagcccg ctgaaaagaa aatttccacg
    13861 ctgggctgat cggatatgtc atttgcaaat accgggagag gtttgagcaa cgacgcgatt
    13921 aaaataattg cgatggggag caatctcttc atgagttaag aatcctttat ttaacgattt
    13981 ttccagtcaa gattttttgc agatgccaat cattggcagg gctgatcttc tgactctttt
    14041 taatactaat tttctttacc aggcttgtaa tgcgctgtgt ggcttgaaca aaataagcaa
    14101 aaatgcctaa tgctccagtt agaacacatg gtctcatgaa tgagggttga aacggttaac
    14161 aacccaggca ggtcaatctg cctgtcagga aacgagcatt tttgccctac ccatacatgc
    14221 acagggaaat caaaattgaa gacaacaagc aaatcatgga gaagtggctt tgaaatccaa
    14281 aagaaaacaa tcaaaatttt ataaaaataa aaagagcttc ccaatttggg gatcagtaat
    14341 catcgcactg attctgcttt tcgtcggtgt ctctttttta aatactgtct tgactccacg
    14401 ggaaaaaatt gaagttaccg gaagtccaaa aattaaagtt gatcaagatt actatgatta
    14461 tggaaatgtc aaaaatggtg gagttccaat ccgaacatca ataaaagtat ccaatattgg
    14521 tgataaacct ttaaactttc aagaagcccc ttacatcgaa ttaatagagg gatgttgccc
    14581 acctgttccg tcaattggca ctttatccct ggctccgggc gagagtacgc aaatcaacgt
    14641 cgaatttttt atgcatggaa cgatgggtgg aatgcacaat ttcaaactgc atttggttac
    14701 aaatgatcca gcagaacccg ataagtcaat aaccatcatt tctaactggg ttgactgatt
    14761 ttcccaagcg atgatagagg gcaagctgat cttatcccaa acaacccatg tgtcttagag
    14821 ttaagcgaat gacatctctt aatgaaccat cgtccattaa tcagggtcgc caaaacggag
    14881 atgcaaaatt gaaagctctt aaagcgagcc tcaaaaagaa gctcagaaaa cggtggctcc
    14941 cggttttatt catctttgta tactccctgg gtatcctaag cggatatggc atttcaggaa
    15001 tggcaaagca aaccgattcc gcgaagatat cgggtacaac cgagatagat gtaatttctc
    15061 gcagcacatt tccccaagat ggttatcgaa ttcctgtgat acttggaaat atcgggtcgc
    15121 aattaattga aatgggggtg atcaaccgca cgcagtttgc tcaagctcac gcagagatgg
    15181 gacgtccatt aagcgctgag caaatgaaag ttttcgatgg cacctatgcg ggcaaagttt
    15241 ttatcaatga agaaaactcc tatttcttac tcaatttctt ttgggcacta ggactggcga
    15301 acaagaatcc cattcttgat tctggattga tccaaaaata ttcgaatggt gaaatagaaa
    15361 tgttcgcatc gacaggcggt tggacatttg caagccatcc catcaatgaa atattctcaa
    15421 gtcaagaaat aatcccctta aacgatgcgc agcagaagat tgtcgaagaa gctgcttcca
    15481 gaatctatcg tccatgctgc aacaacccat ccgattttcc ggattgtaac cacggcatgg
    15541 caatgctggg attgcttgag ctgatgggat cacaagataa aaatttggat gaaatgcttg
    15601 aggctgccaa gtatgtagcc gcatattggt ttcaagagca aactctggcg caggcagtat
    15661 attttaaaaa agcggaaaat aaagaattta aggacgttga tgcaaatagg attctcagtt
    15721 cattgtattc ctcggcatca ggatttaacg aactttatac attcatccta gagaaaaaat
    15781 ggatgccttc ctcaaatatt catctcagta actgcggggg ttagaaatct ctccgattct
    15841 ttctatgcaa tattcagccc acttttccca ttaagatggt ccccaaatgg ggttcataat
    15901 gagttccagc cgatcgccac agcttgagaa ttaggaaatc cgtaccgaaa ttgacgacag
    15961 aagataattc acaaaaatca tctccacaga tacaattacc tggtatctca ccagggaagg
    16021 agtattgtta tgcacatcaa tccaggaccg atttcagagg gtctcataga acgagataag
    16081 aaggtcatct catcttctta tccccgtggg tatccattcg tgatggatca cgggaagggc
    16141 gttgaagtat gggatgtgga tgggaagcgg tatctcgact ttgccgcagg gatcgcagtc
    16201 aattcgaccg gtcattccca cccagaagta gtagctgcca tcaccgaaca agcccaaaag
    16261 ttcattcata tttcatccga tttctaccat caaaaatgga tcgaattagc cgagaaattg
    16321 gatgagattt ccccattcca tctcccagcg atctctttta tggcgaattc cggctccgaa
    16381 gcggtagaga gcgctctaaa gctcgcgaaa tatcacaccg aacgctcaca gttcatcggc
    16441 tttttgggag gatttcacgg tcgtaccacg ggctcccttt cgatgaccgc ttcgaaactg
    16501 atctaccatc gctcatttta tccgctcatg ggcggggtaa cccatgtacc ttaccccaat
    16561 ccttaccatc cgatcttgca catggaagaa ggagaagacg ccggagatgc ggtcatccgt
    16621 tatttggaag aaatcgtgtt tcataagctg ttgcctccca ccgaggtagc cggcattctc
    16681 atcgaacctg ttcagggtga aggcggttat gttttcccgc ccaatgactt cttgcccgcg
    16741 ctgagagaga tctgtgataa atacggcatt ctgctcatcg ctgatgaagt acagtcaggc
    16801 atgggtcgga ccggaaaatg gtggggggtg gatcattatg gcgtggagcc tgatatcatc
    16861 tgtatcgcca aagggatcgc ctctggcatg cccatggggg tcatgctggc aaaagcggag
    16921 cttatgacat ggccgaacgg ttcccatggg aacacttttg gcggaaatcc gctggcttgt
    16981 gccgccgccc tcaaaaccat cgaactgatc gaaaaccaat accttgcgaa cacccagaaa
    17041 atgggcacga tcgcactgga atttttgcag caattgcaga ccgggcacgc ctgcatcgga
    17101 gacgtgcgcg ggaaagggct gatgatcggg gtggagttcg tcgaagaccg gaaaaccaag
    17161 cagcctgctg tcgacctgcg caacgatgtg gttgacctgt gcttccaaaa agggttactg
    17221 acgctgggtt gtggcgacag caccatccgc atcacgccac ctttatgcat taacgaacag
    17281 gaagtaagag aaggattaga aatctttgcg gcggctgtaa aagaggttga atctcattta
    17341 taatctttcc atattaagta aggaaatgga aagatcatgt tccctgattt tcgggttcgc
    17401 cagcgcgatt acttactaga gatctcacaa gcgctcaccc aggaattgga cctggatgtt
    17461 ctgctcaata agattttaaa atacgccatc gaaatgttga gcgggcacgc tggtttgatc
    17521 gccctgcgca gtgaggaaag tggctggcat atccgggttt ctgaaggtat ttctcccgac
    17581 ttcctggaac acatgaaacc ttttctgaaa caaattcctg atgaccagga tccagaaaaa
    17641 ttcgaagttc ctgaagtcaa tcgcgtactc agtgaaatca ctttttccgc caccctgggg
    17701 ctgttgagcg gcgtaggctt acccctcatc acacaacaga ccgtggtcgg cttgatcttc
    17761 atttttcgca gctacgccgg tcttttcacc gccaacgacc gaatccttct tcgcagtttt
    17821 gccacccagg ctgccatcgc tgtgcagaac gcccagcttt atcgcagcgt gatccgcaac
    17881 aagcagcgtc tggacgccct ttttgattct gccgctgatg gtatcttact tttgacctct
    17941 aaccaggtga tcgaacactg caatcccgcc ttcacccgct tagccgccca gccgtccgaa
    18001 gagatcatcg gcaaaccgca cgaccagatc gtccgcttgg agaacaccac tcaagagcaa
    18061 acgcttgaaa aagccattgc gggtggctgg ccgctgacac cccatgcgca attatatgta
    18121 gagggagact tggtgcgccc tgggctgcca tcactgccca ttggcatcac ctacgccccc
    18181 atgctttctc ctgagaacaa gctcaccaat attattgcca cctttcgtga catcaccaat
    18241 ttccgcaagg cggatgaagc caagaccacc ttcatctcca tcgtcagtca tgaattgaaa
    18301 acaccggtcg ccctgatcaa agggtatgtt tgtactttga atcgcaaaga tgttgagtgg
    18361 gacagcgctt tcgtagatga gagcttgaag atcatagaag aagaagccga tcatttgaat
    18421 gaattgatcg aaaatttgtt ggatgccacc cgtcttcaaa ccgggaaatt ttccatccaa
    18481 aaaaccgaca tatccattcc agatatcgct cagcgcctgg tggaacgttt ttcaacccaa
    18541 acggacaagc atacttttaa gatcgatttc ccaaaagatt tcccttcggt gttggcagac
    18601 gatacgcgca ttgaacaggt cctttccaat ctgatctcga atgccatcaa gtactcaccc
    18661 aaaggcgaag tgaccatcag cggagaaacg cgccgcgata aggtcatcgt gtgcgtgagc
    18721 gacgaaggtt ccggggtatc acagcaagat ctgcccttta tcttcgaccg attctatcgt
    18781 tcgcccgacg cagcccgcaa aacgaaaggt accggtcttg gattgtatct gaccaggcag
    18841 atcattgaag cgcacaaggg aagaatctgg atcgatacag agagagataa aggggcgtgt
    18901 ttttgttttt cccttccccg aaatgaataa tcggatggat aattagcatt gtttcttata
    18961 gaaaggaaat cttgaattat gcctaaaacc caagttgcct gcccgcagtc ccgccagccc
    19021 attttggtgg aagtacagca gatctttgat ctggcacagg acccgctcgc aaagcagaaa
    19081 ttgctcaacg gtgcggtgaa tgtgatccat tgcccagcct gcggctattc cggaatgctg
    19141 ggaacgccca ttgtgtacca cgatcctgag aaagaactgt tcctgacttt ctttccacca
    19201 gaaagtgggg ttccgctcaa cgaacaggaa aggcaattgg ggcccatgat caatcgtgtg
    19261 atcgatgcgc ttccagccga gaagagaaaa gcctacattc tgcagcccaa atccatgctc
    19321 acctatcaaa cgctcattga atccgtactt gaagcggatg ggatcaccaa agaaatgttg
    19381 gaagagcaac agaagaagat ccatttgatt cagcgtcttt taacggcgcc ggaaaccgaa
    19441 ctggaaacca tcatcgaaca ggaaaaagaa ttgattgacc tttccttctt cgcactctac
    19501 tctcgattgc tgcaatcagc cgttgcgcaa aatgacgaag aatcagccag gcaattggat
    19561 aaggtgcaga aagtgctttt tgaaaaaacc gaggttggaa aagaactata taagtcatcg
    19621 caagaaaccc agcaagccat caaggagctg caagaagccg gtaaggatgg cggtctaacc
    19681 cgcgaaaaat tgctggatgt agttttgaat gccaaaagtg atgccgcgct ttcagcgatt
    19741 gtcagcctgg ctagaaacgg catggactac acgttcttcc aacttctcag ccagaagatc
    19801 gacgcagcca aagataagga aaaagaaagt ctggtcacac tgcgcgaaaa tctgctcaac
    19861 cttacgtcag agcttgataa aaggatgcag gcagaggtgg ataagaccaa agaactgctg
    19921 gaaagcatcg taaaggcaga gaatatcgaa gccgaagcag aaaagaacct ggagaacatc
    19981 accgaactct tcctgcacgt cgtggaagac gagatcgaaa aagccagaaa acagggcgat
    20041 ctggagcgca tccagaaact ggagcgtgta atgatcgtga tccgtaaggc tatggagccg
    20101 ccccaagaag tgaagctgat cgaagagatg ctggcactgg ttgataagca ggacgaattg
    20161 gaaaaattca tggatgccca tcaggaatcg atcacacctg aactcgtcca gcttctgaat
    20221 ggtattctct cgcaggctgg gaatgcgcaa gatgaaggtt atcagaaatt cgaccaactc
    20281 tataaagctg tgctgcgcta ttctatgaaa aagaaccttg ctgcaaaatg atcgcagggc
    20341 ggctgccaca gccgcccttt catttactct attctttcat ctcttgatcg ggttttgaaa
    20401 acggaaccca taccctcgca ccgtctcgat gagttcgaca tcgggttcat attccgttaa
    20461 tcggtagcgt aagcgacgca ccagggcatc gatcgcctcg ttggatatgc cggcgcctgc
    20521 tgaatcagcc caaacccgtg cgatgatctc tgagcgcggc acgatgttgc cgctctgctc
    20581 atacaggcac agcaacagcg tgtattgttg aatggacaag ggtgggtcga ttttcttatc
    20641 gttcacccac actgcgccat ttcccttatc aatgaacact atacggtatg cctgttcttc
    20701 ggagagcggc atggtggcat ctgaagcgac aaacaagaat tcctggatga gggcaatttg
    20761 gatgaaatct ccgtcaaaga cctgcgcggt tccttcgacc tttctatggt tgatataggt
    20821 accgttctta ctgttcaggt cctctacaaa aatcccctct ggggtttgca agatcctggc
    20881 atgttttcgc gacacctggc ggtcatcgat caccagttcg cattcagcgt ctctcccaat
    20941 gactatctca tcatccaaca gccattgttt gccgttcaat ttcccgttct gggcaatcag
    21001 gagggaggtg ttcttttttt ccatctctct atatataata atacattcaa acctcagagt
    21061 ataattctct tcagaaaaaa gatggcaata tctagaaaac caggcgaggt tctgcaaaat
    21121 cgctatctca ttaaaaagat catcggacgc ggcggcatgg gcagcgtcta tcttgccgat
    21181 gacaccctcc ttgaagggcg acaatgcgca ctgaaagagg tcgaatatga ccgttcgctg
    21241 gatagcgtgc tcatcaaaga agcgcaggag cagttcctgc gcgaagctac catcctcgcg
    21301 cgccttgatc accccaatct tcctaaagtt tctgatttct tcacgatcga gtcctgcgat
    21361 tatctggtaa tggattacgt gcccggcgaa gacctgcgta ccatcatttt gcaagcaaaa
    21421 cagaacaact cttttttgcc gctcagtgat atacgcaatt gggctgacca gttgggagac
    21481 gcgctcagtt ttctacacag tcaggaaccg cccatcgtcc accgtgacat caagcccagt
    21541 aatctcaaga tcacgcccag cgggatttta aagttggttg attttggatt ggtcaagatt
    21601 ctggttccgg atgacgtgac catcacggtt attcaaggtc agggcacagc catctataca
    21661 ccattggaac agtatggcgg agatggcatc cacaccgacg tgcgcagcga catctattct
    21721 tttggtgcca ccctgtatca cctcaccacc aacacgcagc ctgccgatgc gcgcgaacgc
    21781 ttcttgcaac ccggcagact gatgccaccc ggtgagatca atccggcgat ctcttctcaa
    21841 atggaaaaag ccatcttatg ggcgcttcag ctccaccccg acgatcgccc gcaaacggtc
    21901 aagcagttca ccgatgcatt gttggggaat accaagaagt tggtagaaaa gatcaatccg
    21961 cacattgacc atcgcaggct ttcgtcgaat aaaaatgtca aatatttatt ttggatttcc
    22021 gttgggttgc tttttctcag ttttttgatc agcttctttg cctgatgccg taaggaactt
    22081 cttccatatc ttcgactgct gccccattcc aaaacccgcg ccaagcagcg tgaatagact
    22141 cacccataag agaaggtaaa ccaattcaag gaaattggtg cgcgaaaaac tgcccggcaa
    22201 gttcaattgg aaccacaaaa agcatccata ccctgcgatc accgcacaca gtgcaatgat
    22261 cgtcccctcc gttaccgaga agaagcgctg tgtgagcaga tagataccgt aagcaatgcc
    22321 gagaatcgtc aatgtcgcca agaaccactg catggtcccc ggcaagctct ttctgcctcc
    22381 attttcaacg gtcatggtct ggtcgccaaa accgccctgg ttcaccaccg agctggcgct
    22441 gggttgggtg gggacggggg ttggagtaat ctggctgatg acgctacccg cttcgctggt
    22501 aatgtccaga atgaatatgt cagaattcct cgccgggtcg ctctgcgcct tgatctccac
    22561 tctgccgtac gactggatgg gataagacat gctggcaaac ccgctttttg tttgggtttc
    22621 gatgatggtg gactgttcgt tttctcccag atgatcgatc atgaaataga ccaccgtgcc
    22681 atccggcacc ggcaaaccgt tgcggtccaa aatcggaccc gcccttaccg gcaatgcgtc
    22741 tcccactctg aagatcaaat tctcggtccc tgcggtctcg ctttcattcc gcttgctttc
    22801 cgataagtta ttcacgtcaa tatccagcgt gatggtctgg ttcggatcgg ggtaggtcat
    22861 ttcgatcagg tcatacccca ccaccggaat ggaaaccggg gaatgtccct tgggaacgat
    22921 ctcctggaac aacaggcgag cggctgtatc gatgaatacg gatgtcttac tgtataggca
    22981 atagtatgcg tccacgaatg tcaggtcggt tgcatccaaa taaaagggca cattgaacgc
    23041 aaataccacc acatttttgt ctcgcagcat atccgggttt tcagccagta gccgctttaa
    23101 agcgtctgat cctggtcgat ctgcattcac atccagcatg gaaaatatca cccagttcgc
    23161 ttcggaaatg taatttccca catctgctcg gtctgtgggg tcatccaggt attcgctgag
    23221 gtcttcgaac gaataggagc gcaagcgcct ttcaagaacc tgaccggtgc cattttgccc
    23281 gtacaggcgc agcacagtat cctccaatgc gttctttgta aaaatctctt cctcttcgca
    23341 tgtgctgcat tgtgctgccg acatggtatc ggtgatgaaa acgatgcgat cgttcgtggt
    23401 gggtgggtcg ggcagaatgg aagataattc ttcgctggat ggattgatca atgtcgcggc
    23461 atttctggcg atcttcgaca acacatcata cccctgcccg atgatgccca attgctgttc
    23521 tgcgggcagt acgaaagata gcgaaaacac c
//
LOCUS       DAOSDS010000030        21880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1039_length_21880_cov_4.397984, whole genome shotgun sequence.
ACCESSION   DAOSDS010000030 DAOSDS010000000
VERSION     DAOSDS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 21880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21880
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1039_length_21880_cov_4.397984"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLL88_05210"
     CDS             complement(<1..458)
                     /locus_tag="PLL88_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00997.1"
                     /translation="MKKVSAKNYFGLLYLLPLILSVIVLKDRSASIFRQLYIQLRYGF
                     TLSVILISSIVLVTFQIKKNQYRNLLLFTVILLFFSMSLAGVWASGRSEPTMINGLIP
                     IVDGENYYNDALRWLNGGYFSAYSAKRPIFVAFLTTLAKISGQSIQVMQA"
     gene            554..1111
                     /locus_tag="PLL88_05215"
     CDS             554..1111
                     /locus_tag="PLL88_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ00998.1"
                     /translation="MKNDLFSLLIKFSTKWVVITGFIIFFAFSFFFLPGQNKIVETYS
                     AGTGSPDTSFLYTPTELYQMAGAYGAEGRSAYIRARWTFDLAFPIIYTFFLTTSISWS
                     ANRVLKEGNDLHTGNLLPLFAMLFDLCENICTTWVFARYPAYNAFLSMLAPAFTLLKW
                     ICVGFSFLFLAAMLIWLLLDTQKKK"
     gene            complement(1149..2405)
                     /locus_tag="PLL88_05220"
     CDS             complement(1149..2405)
                     /locus_tag="PLL88_05220"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ00999.1"
                     /translation="MTKQRNLIVIGGGPAGLMAAGIAAQNGISTLLLEKMEQPGRKLR
                     ITGKGRCNLTNQTELRTFIAHFGKNGPFLYQPFSRFFIDDLLAFLHKIGVEIVVERGG
                     RIFPTSEDANEVTDALIGWVKAQGAEIRTHSPVREILVHDSVVQRVVLGDGTELPARA
                     VILATGGASYTGTGSSGDGYRIAEKLGHTIVPIRPALVPLKTVGNVARRMQGLSLKNV
                     RATLLVNGKKTTSAFGEMLFTHFGLSGPIILTLSGQVVDALRIGNKVEISIDLKPALD
                     EKKLDARLLRELDAHGKQEFGNMLKALLPQKMIPLCAEGVDIPEEKLCNQITARERDV
                     WLHWLKDVRFSVSESLPLEAAIITAGGVDLSEVDARTMQSKLVQGLFFAGEVLDLNAD
                     TGGYNLQAAFSTGWLAGNAAAEMMNS"
     gene            complement(2437..3732)
                     /locus_tag="PLL88_05225"
     CDS             complement(2437..3732)
                     /locus_tag="PLL88_05225"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ01000.1"
                     /translation="MEELKTENNWKKQFFPFWSSQMLSLLGSALVQFSLVWWLTKETG
                     SATVLAMATMFALIPEIVIQPFAGAITDRIDRKKVIIISDASIALATLVLGIFFFFDK
                     VEVWYIYAIMMIRGIGSAFHFPAEQASVSLMVPRDQLSRIAGLNQAAKGIINIVSAPL
                     GALVMELVGVEGSLLIDVVTAALAIGIVSFVIIPRQPELEKKDGSWFGTVMTDMKDGL
                     RYLIGWKGIMVITVMALIFKIALSPAFSLIPLLVSSYFGGDAAQYSMVEVAVGIGIIV
                     GGSILGVWGGFKRKTYTMFLGGLGIGLGMIALGSIPQDGFYYSLPTLFVIGFMVPMVD
                     GSLMAIMQEKIDNEYQGRVFTMFGSILYLSTPVGLAIAGPVSDALGVQFWFVLAGILV
                     LVTMCGGMAFKSLRNIETDPVINPNKTNGWLASEKNQVE"
     gene            complement(4080..5843)
                     /locus_tag="PLL88_05230"
     CDS             complement(4080..5843)
                     /locus_tag="PLL88_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ01001.1"
                     /translation="MMKKLFSKKFALLVLLIMIAGCIPMSEASVDRKAASVAERTIAQ
                     KEREKQKQEEARQKKREKTKRTYNLADEQVLNISGRSFDDLNPENADGEAMNLISLVF
                     PGLTRYDAESGEFLPVLAKSWEVSEDLLTWTFTLRDDVPWVTYDAETEQIEQVMGEDG
                     DPLYVTAEDVKARIMDVIDPNQYFNNYYLLLSITGAMDYVFNGAAADSVQIETPSDTE
                     VIIHLEEPDSGFLALTELPIFSATPSWNDPYDITMAVNFYGPYVPTDPISFNYNTVTI
                     IKNPFWAGTDGLEDPFLETINIDMQYGQDVLTAFKNGELDAVSLTYEEYLEASSDAEL
                     SDYLVTAPANTGYYLLFFNTGISPIDEATGRKEVASAIDKTSLVALPMEVTGTPLGGI
                     VPDFFRDESFEGIPFDEDFSGSEQENPSASVNYLNFLYIEDGSTFSLAEYLTDQLRSN
                     LGREIYTIPMETYYDLFTNIENNYTYDPGIYLLPLNMMTNDPQEIWDSLSSEIGIKQM
                     FQEDWTADDFANLYNEAKLETDSSKRFKLYEKMEEIMVNEDVMVIPLFWTEQHWLVRS
                     DVGAEILPLYQQLENWARIIP"
     gene            complement(5840..7489)
                     /locus_tag="PLL88_05235"
     CDS             complement(5840..7489)
                     /locus_tag="PLL88_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01002.1"
                     /translation="MESKKASMLKPILISICNLNFLGVGYLLVGQKKRWMIAFGASAA
                     ILLTAYFVNASKNPWLWGCIFLAIIIAMAVDLWLILKKDPTLCDGVLKGNLTLLILAA
                     IFLVVVFGGGFYFYRWLGTDLYWQGVLAYQNDDLDTAFKDLYSVSYLYRLSLNPVVLD
                     AQNLLGETSLIIDSQNRVQAGDYVIASDLVEKFEELYPDSPKMQKVKNIGIDAYIGAA
                     QQLTASGEYEASLEKIQTVREILPVQAEERKTEIDETLAANYLAWGEYEYQQKNYERS
                     IEKFETVTSEYLTSTSANDAYAGAALAHFDYATELEGSKQYDLAWEHYSSILEYYDDS
                     AQYQDAKTQTASMLFDWGKELVSQDHFLLAMEKYDLISEYSDESTLLAQADEEKQATI
                     LLLANDSGEDGQSVLNDAMFSACAGDVVADPAVGIFEDGMKKALPCSAYSVTLSEDLI
                     ADKPGNLFYVVDRYDDERLVQSCDYVTSYDRRTLERWQDYSEIIVIEVKTGKTIYDKK
                     FYGSAPESCPGTYWFGGMTEYLYGDYVDEEKITTWLDEVLR"
     gene            7751..9289
                     /locus_tag="PLL88_05240"
     CDS             7751..9289
                     /locus_tag="PLL88_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOJ01003.1"
                     /translation="MLARVFSCAVLGLDGVIVEVEVDLANGLPGVIIVGLPDAAVQES
                     RERVQSAIKNSGLLFPRKHVRVNLAPASVRKEGPSYDLPIALGILAASDQIPIEVLQD
                     ILVVGELSLDGSVRHVRGVLPMTALAVEKGFRRVLVPETDAAEAALIPNLEVIPIRNL
                     AQLCASFNGTATIEIHKFTEKESLPAWELTDFREIKGQEHVKRALEIAAAGGHNILMI
                     GPPGAGKTLLARALPAILPSMSVQESLDVTRIYSVADLLPKEVPLIHTRPFRAPHHTI
                     SHAGLVGGGNWPHPGEISLAHRGVLFLDELPEFSKQVLEVLRQPLEDKLVTISRARGS
                     LTFPANFQLVAAMNPCPCGYYGDASRPCTCSPSSITKYQKRISGPLLDRIDIHIQVPR
                     VEYQKLSEVRVGEDSSAIRKRVEAARARQQKRFQNTSESGQRAIFSNADMKNADTRKH
                     CSLDAEGQKLMESAMRQMQLTARAYYRMLKLTRTIADLAGEEKITSAHLAEALQYRPQ
                     MLFM"
     gene            complement(9308..10891)
                     /locus_tag="PLL88_05245"
     CDS             complement(9308..10891)
                     /locus_tag="PLL88_05245"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HOJ01004.1"
                     /translation="MMKKHLPVAALCVGLAVDILFWKKTPGISFALFIMLSLILGFWL
                     LHRQRVHPARNSIMLIVPILFFSVFTFIRKDPLTIFLDYALTLFCIIVLTGTFRSGNW
                     TTFSLRDYVINFFQFIGSLLVHPKDELEPVTSPKNKEDPVKNWKPFLAVMRGILIALP
                     ILLIFIALFSSADLVFAQKIDGFLRFFRGEYVGEYLFRGAYVLITAYFFMGLLRHAAS
                     RSQQERAVNPEGSRIKPFLGFTETSIILGSVILLFSIFVIIQFRYLFFGQSNITQTGF
                     TYSEYARRGFTELVVVAVFSLLLLQGISVVAKWENRGQKRIVSGLLIGLACTVLIILV
                     SAFRRLLLYEAAYGFSELRIYAHVFMIWLGILIVAILIFNLIERSRLFTNLVLLVVIG
                     FAASLNFLNVDGLIVRQNVERAVEGENLDIAYLARLSADSVPELVQAFTSDEIPSAVR
                     DEIGAALVCRSAYSGYEDSLKTPWQSFHLSTWRAKRALQLVQTELATYHLIENNAGLT
                     TAVTPTGMRYSCWDTGWVD"
     gene            complement(11058..11528)
                     /locus_tag="PLL88_05250"
     CDS             complement(11058..11528)
                     /locus_tag="PLL88_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01005.1"
                     /translation="MKTFIMYDSVFGNTEKLAQSIALTPSIKKNCVVKRVTETTIEEI
                     YGTELLLVGSPTRGFRPTEAIAAFLKKLPADALRGVKAAAFDTRIPLDSIKPAIFRAI
                     VKRGGYAAPMIAKELTARGATLVADPEGFFVESSEGPLAAGELERAVQWAVALK"
     gene            11635..12105
                     /locus_tag="PLL88_05255"
     CDS             11635..12105
                     /locus_tag="PLL88_05255"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOJ01006.1"
                     /translation="MDTTTLPSCIICQKHRGEITLPGGAIFENELIFVSHASRFRDEK
                     DHYLGHVFIETKRHVAELSQLTEPEAQMVGVYAQRISQALMDVLGVVHVYVFIIGDGV
                     PHFHEHLIGRYPNAPREYWGVKVDEWAEAPHGNDDEIAKVSGLLKKYLQDHSVD"
     gene            complement(12220..13179)
                     /locus_tag="PLL88_05260"
     CDS             complement(12220..13179)
                     /locus_tag="PLL88_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOJ01007.1"
                     /translation="MKKIWIDTDPGIDDTYAIAMLLTDPEIEIMGVSTVFGNVTVEKT
                     TQNARLLLEAAGKEHLTLARGASVPLYVPLDTSPFVHGANGLGDMPLPDAKMEESALR
                     APQAIIDAILNNPHEVTLLPIGPLTNIGMAIILEPKIVELAKEVVIMGGAVHCPGNIT
                     PAAEANFFHDPHAAQIVLSAGWKVVLAGLDVCSKGLIPSKLLEKIGNGRNPLAPYMKG
                     SLPFFQNFLKLFELGDSVTLPDTLASAYLLEPDIFTIKEEYLFVETEGSCMGQSLAVP
                     RGKWYEDPNSNARYAADNHIGLVDVLLDVEQDRFLKLLEQRLV"
     gene            13356..14243
                     /locus_tag="PLL88_05265"
     CDS             13356..14243
                     /locus_tag="PLL88_05265"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ01008.1"
                     /translation="MSKNTIAGLITPILTPFTNDNKLAESLVDPFLQFLSPHINGYFV
                     CGSFGSGMMMETPERKRILELVCASNATKEKMIIVHVGTTSTASTVELAAHAQEQGAQ
                     AIAAVVPYYYPHTDSAILAHFKTLLKATDLPVYLYDYPAYTNQKVGLELFEKLIALGV
                     RGVKDTTGNLDAMKERLTRIPFSQCDYVIGTESLLVPAYAMGIRGCISGLSNAFPELV
                     AGLFNALNLNDKAQILKYQDVIKGVRQLMSRYAKMEAIYAILALRGIDCGKARAPFGF
                     LDAESRESLRNALFSMNLL"
     gene            14265..14873
                     /locus_tag="PLL88_05270"
     CDS             14265..14873
                     /locus_tag="PLL88_05270"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HOJ01009.1"
                     /translation="MKTIEYFNSDGSLREVGSLVSDLHVAGYHCSEALIRALWQHLLP
                     EKNLDESILKMTATLHGGMAESMSSHCGGLTIGILIIGAVYGRSDANGDARYDAALAR
                     GFWQRFLNEFGTSHCSTLKRQEFTDGASSRCTYIMMRSARMLLEYLDELKKNPVQKEE
                     LYYWRVDRTKEPCHEQIALLKPAEEIKAELIKKQGQQQELNQ"
     gene            15036..15563
                     /locus_tag="PLL88_05275"
     CDS             15036..15563
                     /locus_tag="PLL88_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01010.1"
                     /translation="MRMPLKLAIISICCLAIISVACSFPFFQSGEEEIVIPTLAQQVI
                     TVLVTAAPEEDKAAETDTPAPTAVVVMDVDWDDQWTIWMGDSSKGYTINFLIQGNKFS
                     GSTVLTNKNSISFIGTIQESGGTVVGTWENTDGTHGDFTIYMNEGENFFTGHMSGSSA
                     LCGSRDNIKPSACFK"
     gene            15789..16232
                     /gene="mraZ"
                     /locus_tag="PLL88_05280"
     CDS             15789..16232
                     /gene="mraZ"
                     /locus_tag="PLL88_05280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOJ01011.1"
                     /translation="MFFGQYEHTIDEKGRLTIPARFREDLAEGTYITLGFDNNLMCMS
                     KHNLEVLSHKLRTHNIMDPFARKLRRMFYSYGVLIESDKMGRILIPQNLREMVNLDQN
                     VVITGSGDYFEIWSASQWNSESDQLQKSDLKDKKLEYLDLTFEEP"
     gene            16229..17167
                     /gene="rsmH"
                     /locus_tag="PLL88_05285"
     CDS             16229..17167
                     /gene="rsmH"
                     /locus_tag="PLL88_05285"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOJ01012.1"
                     /translation="MMPADQIYPHVPVLNREVLAYLNPATKRKYVDATVGAGGHAEAI
                     LIASRPDGLLVGLDIDHHAVAIATARLRKFSKRASIFNVSYIAMKECLKKMNWDCVDG
                     VLFDLGISSMQVDAAERGFSFNKEGPLDMRFDDHSNFSAYDLVNQWTEEEIASVLWNY
                     GEEPKARAIARAICAARPLNTTTQLAQVVLSAYHGKNSKIHPATKTFQAVRIAVNQEL
                     KALEKGIHLAIDQLCAGGKIAVITFHSLEDRIVKEIFKQESKDCLCPPEQPVCTCHHK
                     ATLSILTKKPVMPSDEEIKTNPRSRSAKLRVAQKIG"
     gene            17174..17641
                     /locus_tag="PLL88_05290"
     CDS             17174..17641
                     /locus_tag="PLL88_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01013.1"
                     /translation="MAKQFVQAYKQTPRRKQIQRLGLYVLPVITLGTIFALYLIFSAQ
                     AATAGLEIHAYLDAKEELQRTIANENTQLAWLLSNTNMIKRAEELGFSQISSSNIFYM
                     VLPSYPGRTVHLTSPAPDGDSEGDIVLNSAYQQSLGDFLRDLLFSNSNDEAEP"
     gene            17638..19452
                     /locus_tag="PLL88_05295"
     CDS             17638..19452
                     /locus_tag="PLL88_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOJ01014.1"
                     /translation="MKYKINARLNLIGAICSFIVIVIIFQLFRIQNSEQYIELSEKAD
                     QTYTYEKRIVYPERGNIYDRWGHLLAGNEEVYEVGVLLQYVKNAKAIADTLSIIPDVN
                     YEKAFETASLEYGNGKNIYGIIADFLPSGVIDALSKIKEEYETLNPEGEDPDLPSLRG
                     MVWYPHLQRSYPENTLAANVLGFYKFRDRKNGEGFYGVEEYYDDILSGPPVTVEIPLD
                     PYMIEETPTLPSGASLVLTIDREIQAMVEEKVDEAVEYYKAESATVIVLNPKNGEILA
                     MVSQPRINLNQYWIFEEELKDTTPFNRAISETYEPGSVFKILTMAAALDSEAIELDEK
                     FSVGQYFEYYGSYIYNWNRKSWGEQDVLGCLQHSLNVCLAKIAVEEMKADTFYSYMQA
                     FGIGRRTNIDLGGEEIWPLSVPGDDGWYPINLATNSFGQGVAVTPIQMATAASAIANG
                     GKMMKPHVVKAIIDDGEYYETSPQVLSVPISEETADLMNTLLTKSLEGGEGSLALVDG
                     YHIAGKTGTAEVPVESGYDSSLSNVSFVGWGPVDDPQFLVYVWIEKPDPEIGYWGSYV
                     AAPVFHDIVEELVVMLNIPPDAVRQSLYAAGSSHDQTD"
     gene            19436..20851
                     /gene="murF"
                     /locus_tag="PLL88_05300"
     CDS             19436..20851
                     /gene="murF"
                     /locus_tag="PLL88_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOJ01015.1"
                     /translation="MIKLTDLFNTFNMKVDERREGLLSEATIDSRRVIPGSLFFAMQG
                     ERVDGHAYISQAVSAGAQVVVMDKESNVDFPVIEIDKVDSSQTVSLPDFPFGIKVNNT
                     LTAMQQIATRQRKCRDIKVIGITGSVGKSSTKELVAEVLSQRFATHKNPGNYNNEIGL
                     PLTLMNCGYGQECVVLEMGFYYPGEIKFLCEIASPEVGIVTNVGTVHAERAGSQQAIT
                     DGKAELIQALPDDGVAILNYDDPFVRPMCKKTRSKVLFYGLSPSADLWADAIQSKGLQ
                     GITFDMHYQESQYHVQVPLLGRHSVQTILRAAAAGLAMGMNWDEIIRGLQKGHSQLRL
                     VAVRTETGALILDDTYNATPESMLAALDLLDELKGHKIAVLGDMLELGAYEAAGHHQV
                     GEKAAGVVNHLITVGKLGRIIADTARKAGLPENAITSVNDSSEAIEILKYNLSADDVV
                     LIKGSHGLRMDTISATLEKTQ"
     gene            20848..21843
                     /gene="mraY"
                     /locus_tag="PLL88_05305"
     CDS             20848..21843
                     /gene="mraY"
                     /locus_tag="PLL88_05305"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOJ01016.1"
                     /translation="MRETSVSIMLVILSFVLSLIWGGPLIKLMRHFKIGKIIRVDGPE
                     SHIKKLGTPTMGGFIIILPVVILTILLNAATVLGLDVSGLSVLVPLGVMMVFSMLGAI
                     DDWEGIRGPRKGLGMSAKTKFLMQTIFALITAFFLKYALHVPELYWPGYPDAIPLGVA
                     FIPIATFIIVGYSNAMNFTDGLDGLASLISITTFTAYGVISSIQGQTFLANFCFTIVG
                     ALLGFLWFNVHPAQLIMGDNGSLALGAAMATVALMTGHWLLLPVIAIIPTSEVLSVAL
                     QVIYFRFSGGKRLFKMAPLHHHFELSGWSETQVVQRFWIINLLFVLIGIAISTVR"
BASE COUNT         6030 a         5296 c         4894 g         5660 t
ORIGIN      
        1 aaagcttgca tcacttgaat gctttgacca gagatctttg ctagagtggt gagaaaagca
       61 acaaagattg gacgttttgc agaatatgca gaaaaatagc caccattcaa ccagcgtaag
      121 gcatcattgt aataattttc gccatcaacg ataggaatca gtccgttgat catggtcggt
      181 tcagacctac cgcttgccca tactcctgct aaagacatac tgaaaaaaag aaggattacc
      241 gtaaaaagca ataaattcct atattgattc ttttttattt gaaaagttac cagaacaata
      301 gaggagataa gaattacaga taaggtaaag ccatagcgca attggatata gagctgccgg
      361 aagatagaag ctgagcgatc ttttaaaaca atgaccgaaa ggattaatgg taaaagatac
      421 agcaatccaa agtaattttt agctgaaact tttttcatag tttcaaaacc accctcatca
      481 ggagaggttg tgtagagtaa gaattactta aaattatact aagataaata agatttccga
      541 ataaagagga tttatgaaaa atgatttatt ttcattactg atcaaattca gcacaaagtg
      601 ggtggtcatc actggattta ttattttctt tgctttctcc ttcttctttc tacccgggca
      661 aaacaaaatt gtcgaaacct actccgctgg aacgggctcg ccagacacct cgtttctcta
      721 tacccccaca gaactctatc aaatggctgg tgcctatggc gcagaaggac gttccgctta
      781 cattcgagca cgctggacct tcgacctcgc ttttccgatc atctacacgt tctttttgac
      841 aacgagcatc agttggtcgg cgaatcgtgt cctgaaggaa ggaaatgatc tccacactgg
      901 caatctgctc cctttgtttg ccatgctttt tgatctgtgc gagaatatct gcaccacatg
      961 ggtatttgca cgataccccg cttacaacgc ctttctgtca atgctggcac cggcattcac
     1021 tctgcttaag tggatttgcg tgggtttctc ttttttgttc ctggctgcca tgttaatatg
     1081 gctattgttg gatacgcaaa aaaagaaata acaaagattg ttcttggttt gacacacagg
     1141 gtgaaaagct agctgttcat catttcggct gccgcattgc cagccagcca gccggtagaa
     1201 aaagccgcct gcagattgta tccgcccgta tccgcattca aatccagcac ctcgccggca
     1261 aaaaataacc cttgaaccaa tttggattgc atggttcgtg catccacttc tgatagatcc
     1321 actcccccgg cggtaatgat ggcagcctct aacggcaaac tttcactgac ggaaaaacgc
     1381 acatctttca accaatgcag ccatacgtcg cgctccctgg cggtgatctg attgcataat
     1441 ttttcttcgg ggatatctac cccttctgca caaagcggga tcattttttg gggcagtaat
     1501 gctttcagca tgttcccgaa ctcctgtttc ccatgcgcat ccagttcacg caacaaacgg
     1561 gcgtccaatt ttttttcatc caaggcaggt ttcagatcga tagaaatttc caccttattg
     1621 cctatgcgca aggcatccac cacctgcccg ctcagggtaa ggatgatcgg tccgctcaac
     1681 ccgaaatggg taaacaacat ttcaccaaag gcggaagttg tctttttacc attcacaaga
     1741 agcgtagcac gcacattctt taagctcaac ccctgcatac ggcgagcaac atttccaaca
     1801 gtttttaacg gcaccagtgc cggccggata ggaacgatgg tatgcccaag tttctcagct
     1861 atgcggtacc catctccgct ggaaccggtg cccgtatacg atgccccgcc cgtagccagg
     1921 atcacagccc tggctggcaa ttccgtccca tctcccagca caaccctttg caccacacta
     1981 tcgtgaacaa ggatttcacg cacaggagaa tgtgtgcgaa tctctgcacc ctgcgccttg
     2041 acccatccga tcaaagcatc cgtaacctca ttggcatctt cactggtcgg gaagatcctg
     2101 ccaccacgtt ccaccacaat ctccacgcca attttatgga gaaacgcaag cagatcatca
     2161 atgaaaaatc gggaaaatgg ttggtataaa aagggtccgt tctttccaaa atgggcaatg
     2221 aaggtacgca attcggtctg gttggtgagg ttgcagcgac ctttgccggt gatgcgcaat
     2281 tttcgtcccg gctgttccat tttttccaaa agcaaagtgg aaatgccatt ttgtgcggca
     2341 attccggcag ccatcaaccc tgccggaccg ccaccgatca caataaggtt tctttgttta
     2401 gtcaaaacga gttcgtacct tcaggtcttt ttttctctat tctacctggt tcttttcaga
     2461 cgcaagccag ccatttgttt tattcggatt gatgaccggg tcagtctcaa tgttgcgcag
     2521 agatttgaat gccatcccgc cgcacatggt gacaagaacc aggatgcccg ccagaacaaa
     2581 ccaaaactgc acgcccagcg catctgatac cggacccgcg atcgccaacc ctacgggagt
     2641 agaaaggtag agaatgctgc cgaacatggt aaaaacccgt ccctgatatt cattatcgat
     2701 cttctcttgc atgatcgcca tcagcgatcc atctaccata ggaaccatga acccgatcac
     2761 aaacagggtg ggcaaactat agtaaaagcc atcttgcggg atagaaccta gtgcgatcat
     2821 tcctaatcca atcccaagcc cgcccaaaaa catggtgtac gtttttctct tgaatccacc
     2881 ccaaactccc aagatcgatc cacccacgat aatcccgata ccgactgcta cctccaccat
     2941 gctgtattga gccgcatccc cgccgaaata actgcttacc agcagtggga tcaaagaaaa
     3001 cgccggtgaa agcgcgatct tgaaaatgag cgccatgact gtgatcacca tgataccttt
     3061 ccaaccgatg agatagcgta agccgtcttt catgtcggtc atgacagtgc cgaaccagga
     3121 tccatctttc ttttcgagtt ccggctgacg tgggatgata acaaacgata caatgccgat
     3181 cgccagcgcc gcagtgacca catcgatcag caaagaacct tcaaccccca ccagttccat
     3241 caccaatgcc cccaggggag ccgaaacgat attgatgatc cctttcgctg cctggttgag
     3301 tccggcgatt ctgctcagtt ggtctctggg aaccatgaga gagaccgacg cctgttcagc
     3361 cgggaaatgg aaagcgctgc caataccgcg gatcatcata atggcataga tataccagac
     3421 ctccacctta tcaaaaaaga aaaaaatgcc taacaccagg gttgccaacg caatggaagc
     3481 atctgaaatg atgatcactt tcttgcgatc aatgcggtcc gtgatcgctc cggcaaaggg
     3541 ctggatcacg atctcgggga tcaaagcaaa catggtagcc atagccagca cggttgccga
     3601 tccggtctct ttggtcagcc accacaccag cgagaactga accaatgcgc ttcccaataa
     3661 ggagagcatc tggctggacc aaaatggaaa gaactgcttt ttccaattgt tttccgtttt
     3721 caattcttcc atttgctcac tcctgtacat caataatatt gattatatat tgagcaatat
     3781 tctatcatta tttaataata tgaatattat aagtacatta ttttactatt attcatattt
     3841 ttgtaaagga ttaatatttt tttagtgttg gtataaactg tcgttctcat gccaacttca
     3901 tcgctaccga cgggttatac agattgttca gaatatatac gtacgtactt ccctttcagg
     3961 tttggaagat gctttcaaga agcaatgaac ggcttcaatg ctgcatgatg gaatatttat
     4021 taaaaaagcc agagcatgaa caacatgctc tggcttttac ttttcatcag cgtaaaagat
     4081 cagggaatga tccgtgccca attttctagc tgctgataaa gtgggagtat ctctgctccc
     4141 acgtcagagc gcaccagcca atgctgttca gtccagaaaa gtgggataac cattacatcc
     4201 tcgttcacca tgatctcttc cattttttca tagagcttga aacgtttcga ggaatccgtc
     4261 tctaattttg cttcattata taaattagca aaatcatcag cggtccaatc ttcttggaac
     4321 atctgcttaa taccaatttc gctgctaaga gaatcccata tttcttgggg gtcatttgtc
     4381 atcatattca agggcagtag ataaattccc ggatcatatg tgtagttatt ttctatatta
     4441 gtaaaaagat cataataggt ttccatcgga atcgtataaa tttctcgacc aaggttgctc
     4501 ctcaattgat ccgttaaata ctctgcgaga gaaaaggttg aaccatcttc aatatataga
     4561 aaattcagat aattcacaga tgcagaagga ttttcttgtt cagaaccaga aaagtcttcg
     4621 tcaaacggaa ttccttcaaa ggattcatcg cggaaaaaat cgggaacgat ccctcccaaa
     4681 ggtgtccctg tgacctccat tggtagagcc accaaagaag ttttatcaat agctgaagcg
     4741 acctcttttc gaccggttgc ctcatcgatc ggactgatac cggtattgaa aaacaacagg
     4801 taataaccgg tgttcgccgg tgcggtgact aaataatcag agagctctgc gtcagacgaa
     4861 gcttccagat attcttcata ggttagcgat acggcatcca gttcaccatt tttaaatgca
     4921 gtgagaacat cctgaccata ctgcatatcg atgttgattg tttccaaaaa tggatcttca
     4981 agcccatcgg tacctgccca gaagggattt ttgatgatcg tcacagtatt atagttgaac
     5041 gaaattggat cagtcggtac gtaaggacca taaaaattca ctgccatggt aatatcgtat
     5101 ggatcattcc aagaaggcgt tgctgagaag attggcaatt cggtcaatgc cagaaaccca
     5161 gaatcaggtt cttcaagatg gataattact tcagtatccg aaggtgtttc gatttgtact
     5221 gaatctgcgg cagcaccgtt gaagacataa tccattgctc ctgtaataga tagaagcaaa
     5281 taataattat taaaatactg atttggatca ataacatcca tgatgcgagc tttcacatct
     5341 tctgcagtta cgtagagcgg atcaccatct tcacccatca cctgttcgat ctgttcggtt
     5401 tctgcatcat aggtcaccca tggcacatca tctcgcagag tgaaagtcca ggttaacagg
     5461 tcttctgata cttcccagct cttcgcaagc acgggcaaga attcacccga ttcggcatcg
     5521 tatctggtta aaccggggaa aacaaggctg attaaattca tcgcttcccc atccgcattc
     5581 tccgggttca gatcatcaaa agaacgtccg ctgatattca gaacctgctc atctgccaga
     5641 ttatatgttc tctttgtctt ttccctcttc ttttgccttg cctcttcctg tttttgtttt
     5701 tctctttctt tttgcgcaat cgttcgttca gctaccgatg ctgcttttct atcgacagaa
     5761 gcttcgctca ttgggatgca accggcgatc attatgagca gaacaagtaa agcaaatttc
     5821 tttgaaaata attttttcat catcgcagca cctcatctaa ccaggtagtt atcttttctt
     5881 catcaacata atcgccatag aggtattcgg tcataccgcc aaaccagtat gtcccagggc
     5941 atgattcagg agcagaacca tagaattttt tatcataaat tgttttcccg gttttcacct
     6001 ctatcacaat gatctcggaa taatcctgcc agcgctccaa cgtgcgcctg tcgtaggatg
     6061 taacataatc acagctctgc acaagtctct catcatcata ccgatcgaca acgtagaaaa
     6121 gatttcccgg tttatcggca atcaaatctt cggaaagcgt gacactgtag gcagaacacg
     6181 gcaaagcttt cttcatccca tcttcaaaaa tgccaacagc aggatctgca acaacatctc
     6241 ccgcacaagc tgaaaacatg gcatcattta gcacactttg tccatcttca ccgctgtcat
     6301 tggcaagcag cagaattgtt gcctgctttt cttcatcagc ttgagccaga agagtggatt
     6361 catcgctata ctcactgatc aggtcatact tctccattgc cagcaagaaa tggtcttgcg
     6421 atacaagctc ctttccccaa tcgaaaagca ttgaagctgt ttgtgttttt gcatcctggt
     6481 attgcgccga atcgtcatag tattccagga tactcgaata atgttcccag gcaagatcgt
     6541 attgtttcga tccctctaac tctgtagcgt aatcaaaatg cgccaaagct gctcctgcat
     6601 aggcatcatt cgcggaagtg cttgtcaggt attcagaagt gaccgtttca aatttttcaa
     6661 ttgatctctc ataattcttc tgttgatact catattctcc ccaagccaaa taattggcag
     6721 ccagcgtctc atcgatctcc gttttccgtt cttcagcttg aacaggtaaa atttcgcgaa
     6781 cagtctgtat cttttcgagg ctggcttcgt attctccaga tgctgtaagc tgctgtgcag
     6841 caccgatgta agcgtcaatc cctatatttt tcaccttttg cattttcggc gaatcaggat
     6901 agagttcttc aaatttttct accaagtcgg aagcgatcac ataatcacca gcttgtaccc
     6961 gattttggct atcgattatc aagcttgtct cgcccagcaa gttttgcgca tccaacacaa
     7021 caggattgag tgaaagtcga tacaaataag aaacggaata aagatctttg aaagctgtgt
     7081 ccagatcatc attttgataa gccaacactc cctgccagta aagatcagtt cccaaccagc
     7141 ggtaaaagta aaagcctccg ccgaacacaa cgaccaagaa tatggcagcg aggatcaaaa
     7201 gcgttaaatt acctttcaag accccatcac aaagtgttgg gtcttttttc aggatcaacc
     7261 acagatcgac agccatggca atgatgatcg ccaaaaagat gcaaccccac agccatgggt
     7321 tcttcgatgc gttgacaaag taagctgtga gtaaaatagc cgccgatgcg ccaaaggcga
     7381 tcatccagcg ttttttctga ccaaccaata ggtagccaac ccccaaaaaa ttgagattac
     7441 agatagaaat caaaatcggt tttaacattg acgctttttt tgattccatt ttccctccag
     7501 gttatttttg aaataagata aaaaaaaata gcgcattctg gatgcgagca tgagtcttct
     7561 gcgctattta aagatattat actaagggcg gaaagaaaat caagaatttg agaatttttg
     7621 accggcgtta cctccaaaat acggtgccat ccgacagaag ccaagtaaag tttctaaaaa
     7681 ggcacagcat tgcgcagatt tcatgcacaa acgttgactt tttcatccca tctcctgtac
     7741 acttaacccc atgctggcac gcgttttttc ctgtgccgtc ctggggttgg atggagtcat
     7801 tgtagaagtc gaggtagatc tggcgaatgg gttacccgga gtaatcatcg tcggcttgcc
     7861 tgatgctgcc gttcaggaaa gtcgcgaacg cgtgcaatct gcgatcaaaa attccggttt
     7921 gcttttcccc cgcaaacatg tgcgcgtcaa tctagcaccc gcttctgtgc gtaaagaagg
     7981 accatcctat gatctgccga tcgcgctggg aattctggca gcttcagacc agatccccat
     8041 cgaggttctg caggatattc tggttgtagg ggagctctct ctggatggtt cggtgcgtca
     8101 cgtgcgagga gtgttgccaa tgacagcact ggcagtggaa aagggttttc gccgcgtgct
     8161 agttcccgaa acagatgctg ccgaagcagc gctgatcccg aatttggaag taatccccat
     8221 ccgcaaccta gcacagcttt gtgcgtcttt caatggcaca gcaacgatcg aaatacacaa
     8281 atttacggaa aaagaatccc ttcctgcctg ggagctaacc gatttccgtg agatcaaagg
     8341 acaggaacac gtgaagcgcg ccctggagat cgcggctgcc ggaggtcaca acattctcat
     8401 gatcggtcca cccggagcgg ggaaaaccct gctcgcgcgc gccttgcctg ctatattgcc
     8461 ctccatgagc gttcaagagt cactggatgt cacccgcatc tattccgtgg ctgatctgct
     8521 gcccaaagag gttccgctca tccatacccg ccctttccgc gcgccgcatc acactatctc
     8581 gcacgccggg ctggttggcg gcggcaactg gccgcacccg ggcgagatct cccttgcgca
     8641 tcgcggagtg cttttcttag acgagttgcc cgaattcagc aagcaagtgc tggaggtgct
     8701 gcgccaaccg ttggaagaca agctggtcac catcagccgg gcgcgcggtt cgctcacctt
     8761 tcccgccaat ttccaactgg tagctgccat gaacccctgc ccctgcgggt attatggaga
     8821 tgccagtaga ccctgcactt gttctccttc cagcatcaca aaatatcaga aacgcatttc
     8881 cggaccgctg ctcgaccgca tcgacatcca tattcaggtg ccacgggtgg aataccaaaa
     8941 gttgagtgag gtgcgcgtgg gtgaagattc ttctgccatc cgcaaacggg tagaagcagc
     9001 acgtgcccgc cagcagaagc gttttcaaaa cacttctgaa tcaggtcaga gagccatctt
     9061 ctccaatgcg gatatgaaaa atgctgatac ccgaaagcat tgttctctcg atgcggaagg
     9121 gcaaaagttg atggaatccg ccatgcgtca aatgcagctc accgcccgcg cgtactaccg
     9181 catgctcaaa ctgacgcgca ccatcgccga tctggcaggg gaagaaaaaa tcaccagcgc
     9241 tcatctggct gaagcgctgc aatatcgacc acagatgttg ttcatgtgaa gcaggcttct
     9301 cttttaccta atcaacccag cctgtgtccc aacacgaata tcgcatgcca gtgggggtga
     9361 cagccgtggt cagcccagcg ttgttctcta tcaggtgata tgtcgccaac tccgtctgca
     9421 ccaattggag agcgcgtttc gcgcgccagg tggaaagatg aaaagattgc cagggtgttt
     9481 tcagagaatc ctcatatccg ctgtatgctg atcggcaaac caaagctgcg ccgatttcgt
     9541 cacgtactgc tgaaggaatc tcatccgatg tgaaagcttg caccagctcc ggtacactat
     9601 cagcggaaag cctcgccaga taagcgatat ccagattctc cccttctacc gccctttcaa
     9661 cgttctgacg aacgataagt ccatccacgt tcagaaaatt aagggatgca gcaaacccaa
     9721 tcacaacaag gagcaccagg ttggtaaaca aacgagatcg ttcgatcaga ttgaaaatga
     9781 ggatagccac tatcaaaatt cccaaccaga tcatgaagac atgggcatag attcgcagct
     9841 ccgaaaatcc gtaagccgct tcatataaca gcaaacgccg gaaagccgaa accagaataa
     9901 tgagcactgt acaagccagc ccgatcagca gcccggaaac aatacgtttt tgaccccgat
     9961 tttcccactt tgcgaccacg ctgatcccct gtaacaacag taaactgaac acagccacta
    10021 ccaccaattc tgtaaatccc cgcctggcat attcagaata cgtaaatccg gtctgggtga
    10081 tattgctttg tccgaagaac aggtagcgga attgaattat gacgaaaatg ctgaagagca
    10141 gaatgacgct gcccagaatt atggatgtct ccgtaaaacc gaggaaaggt tttattctgg
    10201 acccttctgg attaaccgct cgttcttgct ggctgcggct tgcagcatgg cgtaacagcc
    10261 ccataaaaaa atacgcagta atgagaacat aggcaccacg gaacagatat tcccccacat
    10321 actccccacg gaagaatctc aaaaatccat cgattttttg ggcaaatacc aggtcagccg
    10381 aagagaacag agctatgaag atcaacaaaa ttggcaatgc tatcagtatt ccgcgcatga
    10441 ccgccaaaaa tggtttccag ttcttcacag ggtcttcttt attcttaggg gaagtcactg
    10501 gctcaagttc atcttttgga tggacaagca aactgccaat aaattgaaag aaattgatga
    10561 catagtcacg cagactgaag gttgtccaat taccactacg aaatgtccct gttaaaacaa
    10621 ttatgcaaaa taacgtaagc gcataatcca gaaatatcgt gagggggtct ttacggataa
    10681 aggtaaatac actgaaaaac aggattggaa caatgagcat aatgctattc cgtgccgggt
    10741 gaacacgttg cctatgcaat agccaaaatc ccaatatcag gctaagcata atgaagaggg
    10801 cgaatgatat cccaggggtt ttcttccaaa agagaatatc aaccgcaagt ccgacacata
    10861 gcgctgcaac aggcaagtgt ttcttcatca tcagactcct tttgtataga tcaacttgaa
    10921 tcctacctga aaaaactatc caatactatc aagaaagact taccgtttgc agattcacaa
    10981 ggagcaaatg gtgaaaacag atcccagaaa tttcctctct gttatgccca aaatgttcac
    11041 gctttgattg cacagagcta tttcagtgca actgcccact gcacagcacg ctccagttcg
    11101 ccggctgcta atggaccttc tgagctctcc acaaagaacc cttcagggtc ggcaaccaac
    11161 gttgcccctc ttgcagttaa ttctttggcg atcattggag ctgcataccc tccccttttc
    11221 acaatggcgc ggaaaatagc gggtttgatg gaatcgagcg gaatgcgcgt atcaaaggct
    11281 gctgctttca ctccccttaa agcatcagca ggcagtttct tcaaaaaagc cgcaatagct
    11341 tctgtgggtc gaaaaccacg agtaggagaa ccaaccaata gcagttcagt accataaatc
    11401 tcttctatcg tcgtctccgt aacccgcttg accacacagt tcttcttgat ggaaggggtt
    11461 aaagcgatgg attgagcaag cttttctgta tttccaaata ccgaatcata catgatgaac
    11521 gttttcatga taatttctcc tcattgacgc actcaaatca tatacggttt tcattgaaaa
    11581 tagttttcct tcttctgctt atctccccaa aagattggtg tatagtatga aaagatggat
    11641 acgacaactc ttccttcttg cataatatgc caaaaacatc gcggtgagat cacgctccca
    11701 gggggtgcga tctttgaaaa tgagctgatc tttgtttcac atgcttctcg ctttcgcgat
    11761 gaaaaagatc attatcttgg acatgtcttc atcgagacga aaagacatgt tgcagaactg
    11821 tcgcaattga ccgaacccga agcgcaaatg gtcggggtgt atgcccaaag aatctcccag
    11881 gcattgatgg acgtcctagg agtggtgcac gtatacgtct ttataatcgg agatggggtt
    11941 cctcactttc atgagcacct catcggaagg taccccaacg ctccccgcga gtattgggga
    12001 gtgaaggttg atgagtgggc ggaagccccg cacggaaacg atgatgagat cgccaaggta
    12061 tccggtttat tgaaaaagta cctgcaggat cactctgtgg attaagtgtt taatcttcag
    12121 agtttgaccc gccaccaaat tctaaaacta ttgaccggat aagcatgcaa tgaacacaat
    12181 gcttgccggc ttgtttgcca tctcagaacg atctctcaat caaaccaagc gctgctctaa
    12241 taatttcaga aaacgatctt gctccacatc caaaaggaca tctaccaacc caatgtgatt
    12301 atccgcggca tagcgcgcgt tgctgtttgg atcttcgtac cattttccgc gtgggactgc
    12361 cagcgattgc cccatgcagc taccttcagt ttctacgaac agatattcct ctttaatagt
    12421 aaaaatgtct ggttccagca gatatgcgga agcgagggta tccggcaatg tgactgaatc
    12481 gcccagctca aagagtttta gaaagttctg gaaaaagggc agcgagcctt tcatataggg
    12541 agccaatgga ttccttccat ttccaatttt ctccaacagt tttgaaggaa taagcccttt
    12601 gctgcacaca tccaaaccag ccagaaccac tttccatcct gcgctgagca cgatctgagc
    12661 ggcgtgagga tcatggaaaa aattggcttc agccgcagga gtgatattac ccgggcaatg
    12721 caccgcacca cccatgatca cgacctcttt tgccaactcc acgatcttgg gctccagaat
    12781 gatcgccatc ccgatgttgg ttagcggtcc gatcggcagc agggtaacct cgtgcggatt
    12841 gttcaagatt gcatcaatga tggcttgcgg cgcgcgcaaa gcgctctctt ccattttcgc
    12901 atcgggcaat ggcatatctc ccaatccgtt tgcgccatgc acaaacggcg atgtgtctaa
    12961 cggcacatac agtggaacgc tcgcccctct ggctaacgtc aggtgttcct tcccggctgc
    13021 ttccagtaaa agcctagcat tttgggttgt tttttccacg gtaacatttc caaaaacagt
    13081 ggatacaccc atgatctcaa tttctggatc agtcagcagc attgcaatag cgtaagtgtc
    13141 atcaattcca gggtctgtat caatccaaat ctttttcatt cctgcctcgg tttataagaa
    13201 ttcttttcct tgattttaag aatgcgatca ttacctctac cgccctgaaa tcatatccca
    13261 gtttacgcca tgggagaagg gaaaacacgc acatttgtcc ataatgaatg agggaaaatg
    13321 ggtatactta attaacctta taaagatttc tatgcatgag caaaaatacc atagcaggtc
    13381 tcatcacacc cattcttact ccatttacca atgacaataa acttgcagaa agtcttgtgg
    13441 atccattcct gcaatttttg tcgcctcaca taaacggcta ctttgtttgt ggcagttttg
    13501 gttcaggaat gatgatggaa actcctgagc ggaaaaggat cttagaatta gtttgcgcgt
    13561 caaacgccac gaaagaaaaa atgatcattg tgcatgttgg aacaacaagc acagcttcaa
    13621 ccgtagaact ggcagcacat gcccaagaac agggcgcaca ggcgattgct gcggtggttc
    13681 catattatta tccccatacc gattctgcaa tcttagccca tttcaaaaca ctgttgaaag
    13741 ccactgacct tccggtttat ctttatgatt atcccgccta cacaaatcaa aaagtcggat
    13801 tggagctgtt cgaaaaattg atcgcacttg gtgtgcgcgg tgtcaaagac acaacgggga
    13861 atctagatgc aatgaaagag agattaacac gaattccttt ttcgcaatgc gactatgtca
    13921 tcggcacaga atcattgctc gttcccgcat atgccatggg aattcgaggg tgcatctctg
    13981 gcttatcgaa tgcgttccct gaactggtcg caggtttatt caatgccctc aacttgaatg
    14041 ataaggcaca gatcttgaaa taccaggacg tgataaaagg agttcgccaa ttgatgagcc
    14101 gttacgcaaa gatggaagcg atctatgcca ttctcgccct gcgcgggatt gactgcggaa
    14161 aagccagggc gccatttggt ttcctggatg cagaaagccg cgagtcatta cggaatgcat
    14221 tgttttcaat gaatttactg taagcaagaa aggaaaacga gaagatgaag acgattgaat
    14281 actttaacag cgacggttct ttacgcgagg tgggaagtct ggtgtctgac ttgcatgttg
    14341 ccggatatca ctgctccgaa gccctgatca gagcgttgtg gcagcacctg ttaccggaaa
    14401 agaatctcga tgagagcatt ttaaagatga cggcaacatt gcacggcggc atggcagaat
    14461 ccatgagcag ccattgtggc ggcttgacca ttgggatatt gataatcgga gccgtatatg
    14521 gtcgcagcga tgcaaacggg gatgcccgtt atgatgccgc tctagcaagg ggattctggc
    14581 agcgattctt gaacgaattc gggacctcac attgttccac gcttaaacga caagaattca
    14641 ccgacggtgc ctcatctcgc tgcacctaca tcatgatgcg ttccgcgcgc atgcttctgg
    14701 aatatctgga cgagcttaag aaaaatccag tgcaaaaaga agaactgtat tactggcggg
    14761 tggaccgcac caaagaaccc tgccatgaac agattgcgct tttaaaaccg gctgaagaga
    14821 tcaaagctga gctcatcaaa aaacaagggc agcaacaaga actcaaccaa taaggtgcgg
    14881 gtcctttctt aatatgttaa gggcaatatt tggcatgttt cctgcaatga aatagttgtc
    14941 cgtacgagtt acatctttat aaaacggact gtgagagaat attgtatttt taggtgtttt
    15001 caatacaatt atagtgaacg aataggagga acacaatgag aatgcctctc aagctggcga
    15061 tcatttcaat ttgttgttta gctatcatct cggtagcttg cagtttccca ttcttccaaa
    15121 gcggtgagga agaaatagtg atacccaccc ttgcacagca ggttatcaca gtgctggtaa
    15181 ccgcagcgcc ggaagaagat aaagcggcgg agacagatac acctgcaccc acagcagtgg
    15241 tggtcatgga tgtggattgg gacgatcaat ggaccatctg gatgggagat agcagcaaag
    15301 ggtacaccat caacttcctt attcagggaa acaagttcag cggttcaacc gtcctgacca
    15361 ataagaattc catttccttt atcggcacga tccaggaaag cggtggaacg gttgtcggaa
    15421 cctgggagaa taccgatgga acccatggcg atttcaccat ctatatgaat gaaggggaga
    15481 atttcttcac cgggcacatg agcggcagct ccgctctttg tggcagccgc gacaatatta
    15541 aaccatctgc atgttttaaa taatttcatt ccgtatcagc aaaaaaggga agcgatcaat
    15601 acatcgcttc cctttttcat tttcaaaagt cattgaaaat cttatttttc tcttataaaa
    15661 gaaatttaac tatacgggtt gaattactcc caaaaatcat atataatgtg ggagcaagtg
    15721 gagaaaaatg ggtgattatg ggacgccggt attagccggc gttctggtta tatcaagagg
    15781 tagaacagat gttcttcggg caatacgaac acacaattga cgaaaaggga agattgacca
    15841 ttcctgcccg cttccgtgaa gacctcgcag aaggcactta tatcaccctg ggttttgaca
    15901 ataacctgat gtgtatgagt aaacataatc tggaagtatt atctcataaa ttacgcacgc
    15961 ataacatcat ggatccattt gcgcgtaaat tacgccgcat gttctattcc tatggcgtgt
    16021 tgattgaatc cgacaagatg gggcgcatcc tgatccctca aaatctgcgc gagatggtca
    16081 atctggacca aaatgtggtg atcaccggct ctggcgacta tttcgagatc tggtcagcct
    16141 cacaatggaa ttcggaatcg gatcaattgc aaaaatctga tctgaaggac aagaagcttg
    16201 aatatctgga ccttaccttt gaggagccat gatgccagcc gatcagatct accctcacgt
    16261 tccagttctc aaccgggaag tcctcgcata ccttaatcct gccaccaaaa ggaaatatgt
    16321 ggatgccacg gtaggcgccg gaggtcatgc tgaagccatc ctcatagcca gccgaccgga
    16381 tggattattg gtcgggcttg acattgacca tcatgcagtg gcaattgcca ctgcgcgatt
    16441 gcgcaaattt tccaaacggg catcgatctt taacgtttct tacattgcaa tgaaagaatg
    16501 ccttaaaaaa atgaactggg attgcgtaga tggcgttcta ttcgacctgg gcatttcttc
    16561 catgcaggta gacgccgccg agcgtggttt ttctttcaac aaagaaggtc ccttagacat
    16621 gcgtttcgat gatcacagca actttagcgc ttatgatctt gtgaaccaat ggacggaaga
    16681 agagatcgct tctgtcctgt ggaattacgg agaagagccc aaagcacgtg ccattgccag
    16741 agctatttgc gcagctcgcc cactcaacac caccacccaa ttggcgcagg tggtcttatc
    16801 tgcataccat ggaaagaact caaagatcca cccagcaacc aaaacctttc aagcggttcg
    16861 cattgcggta aatcaggaat taaaagccct ggaaaaaggc atccatttgg caatcgacca
    16921 gttatgtgcc ggtgggaaga tcgctgtgat cacttttcat tcactcgaag atcgcattgt
    16981 gaaagaaatt ttcaaacaag aatccaaaga ttgcctgtgc ccaccagaac aacctgtttg
    17041 cacctgccat cataaagcta cgttgagcat ccttaccaaa aagccggtta tgccttcaga
    17101 tgaagagata aaaacaaacc cgcgttcacg cagcgcaaaa ttaagggttg cacagaaaat
    17161 cggatgaatg accatggcta agcaattcgt tcaggcatat aaacagaccc cgcgtcgaaa
    17221 gcaaattcaa agattgggtt tgtatgtgct acccgtgatt acactgggaa cgatcttcgc
    17281 actatatttg atctttagcg cccaggcagc cacagccggt ctggagatcc atgcttacct
    17341 tgacgcgaaa gaagaattgc aacgcaccat agccaacgag aacacccagt tagcctggct
    17401 gctttcaaac acgaacatga tcaaaagggc tgaagaattg gggttcagcc agatatcctc
    17461 ctcgaatatt ttctatatgg ttctgccttc ttatccgggc cgcacagtcc acttaacctc
    17521 tccggcacct gacggggatt ccgaaggcga tattgtactg aattccgcct atcagcaatc
    17581 gctgggggat tttctcaggg atctcctgtt ctccaattcc aacgatgagg ctgagccatg
    17641 aaatacaaga ttaacgctcg cctcaatttg atcggcgcga tttgctcatt cattgttatt
    17701 gtgatcattt ttcaactctt tcgcatccaa aacagcgagc aatatatcga attgagcgaa
    17761 aaggcggatc agacatatac ctacgaaaaa aggatcgtct atcccgaacg tggtaacatc
    17821 tatgaccgct ggggacattt gcttgctgga aatgaggaag tttatgaagt aggtgtgctt
    17881 ctccagtatg taaaaaatgc caaagccatc gctgacaccc tatcgataat tccggatgtt
    17941 aattatgaaa aagcattcga aaccgcttcg ttagaatatg gcaatgggaa gaatatctac
    18001 ggcatcatcg cagattttct gccttccggt gtgatcgacg ccttgtcaaa gatcaaagaa
    18061 gagtatgaaa ccctcaatcc tgaaggggag gatccagacc tgcccagctt gcggggcatg
    18121 gtctggtatc ctcatttgca gcgcagctac cccgaaaaca cccttgcagc caacgtcctg
    18181 ggtttctata aatttcgtga tcgaaaaaac ggagaaggct tttatggggt ggaagaatac
    18241 tacgatgata ttctttccgg tccaccggtt acggtagaaa taccgctcga tccctacatg
    18301 atcgaagaga cccccaccct tccctccggg gcaagcctgg tgctaaccat tgatcgcgag
    18361 atacaagcca tggttgaaga gaaagttgat gaagctgtgg aatactataa ggcagagtcc
    18421 gctaccgtca tcgtgttgaa tccgaagaac ggtgaaattc ttgccatggt ttctcagcct
    18481 cgtattaacc tcaatcagta ttggatcttc gaagaagaac tgaaagacac caccccgttc
    18541 aatcgagcga tctcagagac ttatgagcca ggctcggtat tcaagatctt gaccatggca
    18601 gccgctctgg attcagaagc gatagaactt gatgagaaat tctccgttgg acaatatttt
    18661 gagtattatg gcagttacat ctataactgg aacagaaagt cttggggaga gcaagacgtg
    18721 ctgggatgcc tgcaacactc tctaaatgtc tgcctggcaa aaatcgcggt tgaagaaatg
    18781 aaagctgata ccttttactc ttatatgcag gcattcggca tcggtcggcg caccaatatt
    18841 gatctcggtg gagaagaaat ctggcccttg agcgttcccg gagatgatgg ctggtatcct
    18901 atcaacctgg caaccaactc tttcgggcaa ggagtggcgg tcacgcctat ccagatggca
    18961 acagccgctt cagccattgc caatggtgga aaaatgatga agccccacgt ggtgaaagcc
    19021 atcattgatg atggtgaata ctatgagacc tctccgcagg tgctgagcgt gcccattagt
    19081 gaagaaaccg ctgatttgat gaatacgttg ttgacaaaat cgttggaagg cggagaaggg
    19141 tccctggcac ttgtcgacgg ataccacatc gctggtaaaa caggcacggc tgaagttccc
    19201 gttgaaagcg gatatgattc atctttatcg aacgtttcat ttgtagggtg gggtccggtg
    19261 gacgatcccc aatttctggt ttatgtctgg atcgagaaac ccgatcctga aattggttac
    19321 tggggttcct acgttgccgc tcccgttttt catgatattg tggaagaatt ggtcgtcatg
    19381 ctaaatattc cacccgatgc agtccggcaa tcactgtatg cagcagggag ttctcatgat
    19441 caaactgact gatctgttca ataccttcaa tatgaaggta gatgaaagaa gagaaggttt
    19501 gctttcagaa gcgaccatcg attcacgacg tgtgatccca ggctcgttat tctttgcgat
    19561 gcaaggtgaa cgcgttgacg ggcatgctta catttctcaa gcggtctctg caggggctca
    19621 ggttgttgtg atggacaagg agagcaatgt tgactttccg gtgatcgaaa tcgataaggt
    19681 tgatagctcc caaaccgtta gtctgcctga ctttcccttt gggatcaaag tgaataacac
    19741 attaacagct atgcagcaaa tcgccacccg ccagcggaag tgccgggata tcaaagttat
    19801 cggcattact ggcagcgttg ggaaatcttc gacgaaagaa ctggtcgcag aagtgctttc
    19861 tcaacgtttt gccacgcata agaacccggg gaactataat aatgagatcg gactcccact
    19921 caccttgatg aattgtgggt atgggcaaga atgcgttgtc ttggaaatgg gtttttatta
    19981 ccccggcgaa attaaatttc tatgcgaaat agcctcgccc gaagtcggga tcgtcaccaa
    20041 tgttgggacg gttcatgcag aaagagctgg ttctcagcag gcgatcactg acggcaaggc
    20101 agaattgatt caggcactgc cagatgatgg ggtggctatt cttaattatg atgacccatt
    20161 cgtcagacca atgtgtaaga agacaaggtc aaaagtgctt ttctacggtc tctctcccag
    20221 cgctgaccta tgggctgacg ccattcaaag caaaggcttg cagggcatta ccttcgatat
    20281 gcactaccag gagtctcagt accatgtgca ggttcccctc ctcggtcgcc attcggtgca
    20341 aaccattttg cgagcagccg ctgccggttt agctatgggg atgaattggg atgagatcat
    20401 tcgcgggctt caaaaaggac attcacaact tcggttggta gcagtgcgca cggaaaccgg
    20461 tgcattgatc ctcgatgata cctacaatgc cacacctgaa tccatgctgg ctgctctgga
    20521 cctgctggat gagctgaagg gacataagat tgccgtgctg ggtgacatgc tcgaactggg
    20581 tgcatacgaa gcagcgggtc atcatcaggt tggggaaaaa gccgccgggg tcgtgaatca
    20641 tttaatcaca gttggaaaat tgggtcgtat cattgccgac acagccagaa aagcaggtct
    20701 tccggaaaat gccattacct cggttaacga ttcatccgaa gccatagaga ttctgaaata
    20761 taacctgagc gcagatgacg tggttctgat caaaggttct cacggattgc gtatggatac
    20821 catctcagct acgctggaga aaacacaatg agagaaacaa gcgtatcgat catgctggtg
    20881 atattgagtt ttgtcctttc tttgatctgg ggaggaccgc tcattaaatt aatgcgccac
    20941 ttcaagatcg gaaagatcat ccgtgtggat ggtcctgaaa gccacataaa aaaattaggc
    21001 actcctacca tgggtggttt tatcatcatt ctcccagtag tgatattaac cattcttctt
    21061 aacgctgcca ccgtattggg tttggacgta tccgggttat ctgttttggt tcccctgggc
    21121 gtcatgatgg ttttcagcat gctgggcgcc attgatgatt gggaaggcat tcgcggaccc
    21181 cgtaaggggt tgggaatgtc agcaaagaca aagtttttga tgcaaaccat tttcgcattg
    21241 atcaccgcct tcttcctaaa atacgccctc catgtgcctg agttatattg gcccggttat
    21301 cccgacgcta ttcctcttgg agtcgctttc attccaattg caacctttat cattgtcggc
    21361 tattccaatg ccatgaactt tacggatgga ttggatggat tggctagttt gatctccatc
    21421 accacattta cggcgtatgg agtgatctca tcaatacaag gacagacctt tcttgccaat
    21481 ttttgtttta cgatcgtcgg ggcacttttg ggttttttat ggttcaacgt acaccctgcc
    21541 caactgatca tgggcgataa tgggtcgttg gctttagggg ctgccatggc taccgtagct
    21601 ctgatgacag ggcattggct tttgctgcct gtgatcgcca ttataccgac cagtgaagtc
    21661 ctgagcgttg ctttacaggt catttacttc cgtttctccg gaggaaaacg gttgtttaaa
    21721 atggcgcccc tgcaccatca ttttgaattg agtggttgga gcgaaacgca agtagttcag
    21781 cggttttgga tcatcaatct gctgtttgta ttaattggaa tcgctatcag cacggtgaga
    21841 tagagatgag agattttaga ggaatgcatg tgcttattct
//
LOCUS       DAOSDS010000031        21331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1070_length_21331_cov_5.569327, whole genome shotgun sequence.
ACCESSION   DAOSDS010000031 DAOSDS010000000
VERSION     DAOSDS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 21331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21331
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1070_length_21331_cov_5.569327"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(246..1280)
                     /locus_tag="PLL88_05310"
     CDS             complement(246..1280)
                     /locus_tag="PLL88_05310"
                     /inference="COORDINATES: protein motif:HMM:NF016894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008671 - 2-dehydro-3-deoxygalactonokinase
                     activity [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HOJ01017.1"
                     /translation="MNIITIDCGTTNSRISVVDSNARLLYHASARVGVRDTAISGSNK
                     ALKEALRSLFEQVLYETKFQISDIHCILSSGMISSEIGLQEVPHLWAPCSMDALAGNL
                     HPYVDSTIFPASIPVYFIRGIKNAFDPASVQLQDLDALDFMRGEETQIAGLLENSKVP
                     VPCCVIVLSSHTKFIAVDAQRTILGSATTLSGQLYDALLKETIIGKSLSAEMEEDGGF
                     FDTRIAEIAKSEIETSGFLRGLLLVRFMDTLLQAPLRERRLLIESLIAAEDLCTLPSF
                     ERLVGQTNQSFVLIGRPQRCRVYEYLLQQIPGHSHCSVQCVTEPDEIDMLSVRGALVI
                     AKKAGIIQTN"
     gene            complement(1351..2121)
                     /locus_tag="PLL88_05315"
     CDS             complement(1351..2121)
                     /locus_tag="PLL88_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ01018.1"
                     /translation="MKNSRTINRQDHILHLLEINDSISIRELSKELDVSEWTIRRDVA
                     ELHNRQIIDRYHGGIRITNHAKDDLTFKEDSDLFSERNRIGIKAAKFLKEGSNVIIGS
                     GSTVRFVAKALARYDKKLTIYTNSIEVCYELANKINYKVVCTGGNVHGDFFTLDGAIT
                     EKLLLSNYFDTGVIGVSGLTIEEGLTIKSQLSALTVSIMIEHSDNLIIVADHSKFGKC
                     YPNRIGDLRSINTIITDSMPPPEFCEFFSKNNIRLILA"
     gene            2276..2722
                     /locus_tag="PLL88_05320"
     CDS             2276..2722
                     /locus_tag="PLL88_05320"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ01019.1"
                     /translation="MDSLKNALPAEQINLNVSVNDWQEAVREAGQLLLEAGAVSPQYI
                     DAMIQTAKELGPYIVIAKGIAMPHAASEAGAKRAALSLVKLAHPIEFGNPDNDPVELV
                     IGLAAIDGETHMNTMRMLAELIIDRERVSRLFAAQTKEEILQVIAQ"
     gene            2735..4057
                     /locus_tag="PLL88_05325"
     CDS             2735..4057
                     /locus_tag="PLL88_05325"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ01020.1"
                     /translation="MLEIKTLTEVDVLVVGGGAAGCLAATAAARRGRKVLLVEAMSAL
                     GGSRTWMGVDSFNGIYSPGTKSYQLVGGISFEIAEKLLTQGGAFLRQNTFGSGPVVTY
                     EIEKLKILYEDLVIRSGGQVLFYTNAVDVECKDGKIDSVIICNKSGLSRVKAQIVIDT
                     SGDLDIGAKAGEDYELAGDHDNPVQSLTTIFYMANVDNEKAFSITQEERTRIMQQAHE
                     SGKYTLTRIGGSIHPTPNPGFVHANLTRIPNINAIDPFAISAAEIEGRKQVQEYVRFL
                     INEYPGFENAYLASTACGIGIRETRRLKGEYVLTGDDVRKGAKFEDAVACCSAPIEDH
                     VAGKSVRWQYLASDDYYQIPYRSLLPVKNKNLIVAGRCLSATHDGQASARNSAQCMAM
                     GEAAGLAAALCNESGKYPNELDSGLLKKELTEQGVLLAPQEVNLKGLL"
     gene            4057..5019
                     /locus_tag="PLL88_05330"
     CDS             4057..5019
                     /locus_tag="PLL88_05330"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01021.1"
                     /translation="MNVIRTVLGDIPADQLGFTDAHEHLIIDRDYVLTLNPDYRLDSV
                     EKTSKEVKSFLDAGGRSFVEMTCLAFGRSAKKLIEVSRITGAHIVASTGFHRPNFYMD
                     SHWRFLYSVDQIARILVEEIEMGMDENQYNGPIIDRTDAKAGVIKIATGYFNIKSEDL
                     VTMEAAAIAHNETGAPIITHTENGTLGLEQVETLGSYGVAPQNIIVGHYDRNPDFYLH
                     QELAQTGCYLEYDTPSRRKYFPEANFIDLVRKMVDAGFEKQILWGGDLSRRSYQTAYG
                     GQPGLAYILNKFIPRMRAEGFSDVTINNIFIHNPSHAFCIAKKK"
     gene            5036..5341
                     /locus_tag="PLL88_05335"
     CDS             5036..5341
                     /locus_tag="PLL88_05335"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ01022.1"
                     /translation="MAKKMKIVTVCGCGLGSSLMAKTSIEKIMKEFGVMASIEASDAG
                     TAKGYPSDLIVTTTMLKPRVGEVPGTPVVAVKSFMNVDELREVMQPHVEEFKKKNNM"
     gene            5487..6779
                     /locus_tag="PLL88_05340"
     CDS             5487..6779
                     /locus_tag="PLL88_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HOJ01023.1"
                     /translation="MTVINFLINEVFVEAPVFLGLVALIGLLLQKKTGAEVLEGTIKT
                     IVGMIILNSGIGVFLGSLSPLTTLLNSSFGITGVLPDCFGPYGVAMQTYGVEIPMAFV
                     LAFVLHLLLVRILPFKNTKNVFLTGHIMLFNASIMLLVGRSALGLTGGAQIAFSAVMT
                     ALFWTFMPAIARPITDPVTDKRFTLGHLQTLDLPVGKWFGGLAKSKRADELELPGIFN
                     LFSDYTVLLCVLMPVMYIVIGLTAGKAAVDSISDGGNWVIWLIMQGITFAAGIAIVLY
                     GVRMFLAAMIPAFEGISKKLLPGTQPALDSPVFFQYSPAGSMLGFVSCFVTMVIFTLV
                     QVAIGSPVVIFPGPIFFFFDGALAGVFGDIHGGWVGAIIAGAVIAIVINVAALVTYPF
                     QAEFFAGTGLMFGGGDYLIWSPIFWLLKFIGGALGLTA"
     gene            6929..7513
                     /locus_tag="PLL88_05345"
     CDS             6929..7513
                     /locus_tag="PLL88_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ01024.1"
                     /translation="MNMIVMNATPKNIAHQAIYDWVNNELGNGTIMDIGSELGFGLHR
                     LASSNRLVIGLDIQLDELTFSKTSKHFHNKNYCHICADGMVIPFSDNSCNGLCMINVL
                     HLVHDLVKVLEECWRILSPQGLLIATIPTDYNLPDEWRIPSEKEYFESILKRAFPSVE
                     FPQEINSLEKDSALFQKQTESGLLTAVCRKNLFS"
     gene            7546..8178
                     /locus_tag="PLL88_05350"
     CDS             7546..8178
                     /locus_tag="PLL88_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOJ01025.1"
                     /translation="MNRFIHSGIMAILRADDPKNIIPALEALVESGIDTVEISLVTPD
                     AVKIIQELHKSLGSKIVLGAGTVLDAESARAAILAGAEFVISPVLSLDVLKISKRYGK
                     MAFAGAFTPTEALTAWENGSDAVKIFPAMPAGPEYIKALHAPLPQIPLIPVGGVTLEN
                     MEAFMDAGCIAVAASSSLVSNDLIKEGKLDEIKHNAKLWISEMQRIREKK"
     gene            8195..9079
                     /locus_tag="PLL88_05355"
     CDS             8195..9079
                     /locus_tag="PLL88_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ01026.1"
                     /translation="MKLKGIIPPILTSFDEKGKIDFKRLEKFAEFLRPHVQGFYVNGT
                     YGSGVLMNVAERKSVFEALSSYSDGSFELIAHVGTTNLRDSIELAQHAEIHRALAVAA
                     VPPFYFRYSEESLFAFFKDLIQSVAVPVYLYDNPGATGNPLSADLINRLAAVGLAGVK
                     DSTFDISKTYMVMRTIKKEGFDVVIGSESLFLPAYMQGSRACISGLANTMPELMQKLF
                     CAAESNDFLQATTLQRQVLEMWDILHYGPSTATAYAMLKVRGMDAGFPRRPFLPIGKD
                     LYEKIESAMKANEAIWKI"
     gene            9057..9806
                     /locus_tag="PLL88_05360"
     CDS             9057..9806
                     /locus_tag="PLL88_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ01027.1"
                     /translation="MKRFGRFSFYKNASIAMIMKPDLTNHPIKAVLFDMDGVLVDSIP
                     IHIQSWNEVLLQKGLPEYSTDLYFSALGRTSLDMVRTYCRENQIMLTESQMQDILSQK
                     EDIFQKNIGEYASTTPGVIDWLDYCKCLGILCAVVSSSTMTNIIHVLHSLHIADFFSA
                     IVSGANFPASKPDPRVYQNAAASLGMPAQFCLVVEDTPTGIQAAKHAGMVCCAVTTSY
                     PKHFLENADLVLNFLSDADPKILFLNERRKK"
     gene            9803..10186
                     /locus_tag="PLL88_05365"
     CDS             9803..10186
                     /locus_tag="PLL88_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01028.1"
                     /translation="MNAILLRFMIVIFSLVLLIFLFARFAPKAWSKRIYGTIVWLIDT
                     AFIYFVNFFIFFKPWKEHKEKIGIYVWLFLVFIIVRYLCAEQPLTTEIKSPYLAFIGY
                     IYFISFLYMIKFIFEFVIKDIKTTD"
     gene            10215..11015
                     /gene="trpA"
                     /locus_tag="PLL88_05370"
     CDS             10215..11015
                     /gene="trpA"
                     /locus_tag="PLL88_05370"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOJ01029.1"
                     /translation="MKKIDKRIQDILEKGEKVLVSGVPVGYPNLEETRKLVEVYIRSG
                     MDIVEFSMPTRNPYIDTQIIADSNVKALDLEPDLNHYFETLHIIRKDFPNEPFYMMAY
                     ADLITQFGLKNFVDQLVELEIDGVELPDKDEMVPELASEMEALFDQSGIYRIYILHHP
                     YNEAFFNRIKEKTAGFLLLQSIADEEGNRPNVNPENKDLIAKIKKETDVPVVLGYGIK
                     NTDRVREAVSLGADGVLVGTSMVEYISQGDYQKFSQFIRSLKEATRNT"
     gene            11528..12436
                     /locus_tag="PLL88_05375"
     CDS             11528..12436
                     /locus_tag="PLL88_05375"
                     /inference="COORDINATES: protein motif:HMM:NF015159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOJ01030.1"
                     /translation="MRTTVSRKQSNKPIKFTVIVTALVLSIMLVSACTATAAEATEAV
                     TADVSTESTSAAEPAKEPIKIKIGVSPVPHGDIITFIKENLAESAGLDIEIIEFTDYV
                     QPNLALADEEIDANYFQHVPYLDDFNQEHSTDLVAVAAVHIEPLGIYSKTITSLDEIS
                     DGAVVAIPNDATNAGRALNLLAANGLLTLKEDVGYSATVNDIVENPKNLQIRELEAAQ
                     LVRSLDDTTISVINGNYALEGGLTPATDALALESGENNPYANVLVVKSGHENDEGIRI
                     LSELLTSAEVKQFIQDNYNGSVIPAF"
     gene            12547..13620
                     /locus_tag="PLL88_05380"
     CDS             12547..13620
                     /locus_tag="PLL88_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ01031.1"
                     /translation="MNSILQEGVNNELQERKTLSWSSINPAKAKRNMILIRNLSKIYE
                     QNGQSIQALDDVSLSIPRGEIFGVVGPSGAGKSTLIRCVNLLEKPTSGQIVIDGQDLT
                     KLSNSELRQARSQIGMIFQHFNLLSSRTVAGNVLFPLEIIGYEKSQRTARVEELLELV
                     GLSDKINAYPAQLSGGQKQRVGIARALATEPRILLSDEATSALDPQTTTSILNLLKDL
                     KRRLGLTILLITHEMEVVRQICDHVAVLQDARIVEQGSIEELVMKPHSLLSQAFFPPV
                     EKVISVPDKTIITILFIGKLADQPVLSKLVKAFDVEVNILGGSIQNIGGKRMGHLQIE
                     ISGKEVEQALNYLQELGLHVEVQ"
     gene            13624..14280
                     /locus_tag="PLL88_05385"
     CDS             13624..14280
                     /locus_tag="PLL88_05385"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HOJ01032.1"
                     /translation="MDWSIWLFNLSKGTVETIYMVGISTVFTALFGLPLGILLVITDR
                     NGLLPSPTVSDILGVIVNISRSLPFIILLVVVIPITRFVVGTTIGATAAIVPLTLAAI
                     PFSARVAESGLREVDRGLVEAAQAMGCSVWQIITKVLIPEALPSLILGITLTIISLIS
                     YSAMAGAIGGGGLGDLAIRYGYQRFETEIMIATVILLIILVQGIQVLGNHLAKKVLKR
                     "
     gene            14341..15507
                     /locus_tag="PLL88_05390"
     CDS             14341..15507
                     /locus_tag="PLL88_05390"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HOJ01033.1"
                     /translation="MAKSQKFETLAIHAGQPPDPTTAAVITPIYQTSTFAQEQVGVTK
                     GFDYGRTANPTRLALEQCLAALESQKRGQAAYGLAYSSGMAAIDNLLRLIKPGEQVIA
                     SNDIYGGTYRLFERVLKEYGIQFTYTDTCDLQKIANCIQPETRLIWLESPTNPMLKIA
                     DIAGISKLVKSHPNRIRIAVDNTFASPYLQQPLMLGADFVVYSTTKYLSGHSDVIGGA
                     IISQDKDDYDKLKFLQNAIGAIPGPFDCWLTLRGIKTLGIRMERHSENAKYIAEFLEK
                     HPKVERTLYPGLPNHAGYEVAKKQMRSFGGMLSIILKDGAEAARRMVEKTQIFTLAES
                     LGGVESLIEIPAAMTHASVAGSPLEVPAGLVRLSVGIEAIDDLLEDLDQALQYA"
     gene            complement(15576..17024)
                     /locus_tag="PLL88_05395"
     CDS             complement(15576..17024)
                     /locus_tag="PLL88_05395"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HOJ01034.1"
                     /translation="MNKQGMNQMTMLQEKRPEVVGNILETIGHTPLIHLNKVVSEKIE
                     AAVYAKAEYFNPAGSIKDRIGLSIIEDAEREGRLLPGGTIVEATSGNTGAGLALVASV
                     KGYRCVFVMPDKMSDEKVRFLRAFGARVVITPTAVSPEDPRSYYNVAARIVRETPNSI
                     LANQYHNPANPDAHYHFTGPEIWGQMDGKIDALVAGMGTGGTISGIARFLKEQNPDVK
                     IVAVDILGSLLYDTWKLGRVPTDPHPKTYKIEGIGEDFLPSTLDLSLIDEVVQVDDRE
                     SFQMTRRLVREEGIFAGGSSGSAVAGLLKSKIVRSFKTDQNVVVILPDSGNRYLSKIF
                     DDNWMSENGFMPVAKITDSIALLLETSNKIPLVVTRPDEEIRAVVDKMNRYDISQLPV
                     VDEDGRLVGLVAEADVLDHLLHASHERDPLESIAKIVNPKVPTASIDESIENILPIFK
                     KSEVVVVVNVTNQPVGMITKIDLIEYLTHKGD"
     gene            complement(17385..18119)
                     /gene="deoD"
                     /locus_tag="PLL88_05400"
     CDS             complement(17385..18119)
                     /gene="deoD"
                     /locus_tag="PLL88_05400"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOJ01035.1"
                     /translation="MPTIPTPHIEAVSTDEIAKVVLMPGDPLRAKKIAEDYLDDVKQF
                     NQVRGMLGFTGMYKGKRLSVMGSGMGFGSIGIYSYELYQFYDVQTIIRIGSCGAWQPE
                     LNIMDIILVDGSFSTETFARDAHGIEDKVIKGSPRISKIVRQVADQKGIKVHEGNVVS
                     DNVFYTTVDIDWQPVYKENKVIASEMESFALFANARAVKKEAACLLTVSDSPFRKGEN
                     LSAEARQNAFNDMMLLALETAVLSFR"
     gene            18420..19019
                     /locus_tag="PLL88_05405"
     CDS             18420..19019
                     /locus_tag="PLL88_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01036.1"
                     /translation="MKNKIGKIISIISVAPLIALYVLTALFLHDANLFGNNALWVGIS
                     IFFLTLMPLSAYLLEHVLPAFKTAGRKGERKLAFAMCIAGYVLGTIVSFVFHAPLAVK
                     MVFTSYMVSGGILALVNGLIKFKASGHACGVAGPCVVLLYFMGAKVWYIILTLAPVFW
                     ARITMGRHSLKELISGAMIGMGATGLTIAAFLFVSKLIA"
     gene            complement(19094..19972)
                     /locus_tag="PLL88_05410"
     CDS             complement(19094..19972)
                     /locus_tag="PLL88_05410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HOJ01037.1"
                     /translation="MSEAIERTQNRLDNLRAIEPLISSLRILSQSTMLMALNKKTMLQ
                     DYKQEYFKILASLSSTSSSVIELSVPLPVERDPNKSILVVLGSDRGLCGPYNKNLAAG
                     TIDWFNTKSGEKRILAFGTKIQQALQQLDLQFEHYSALGSGSLPRYDLAHRFIYSWLQ
                     AYQEGSLNAVDILSFRQTPKGNYQPLVTPLLPISKHFEEVHDYAPAWPPPIIEGDPVE
                     IARRTIEYLTAINFYDIILESIVAENVTRFRLLDNAKENTQDLIEELTMVVQTQRRLA
                     ITRQVQELAVGAGLLR"
     gene            complement(19975..20292)
                     /locus_tag="PLL88_05415"
     CDS             complement(19975..20292)
                     /locus_tag="PLL88_05415"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HOJ01038.1"
                     /translation="MKMIMAVIPHGSGKIVLDELVNAGYKVTYSETRGGLLKQSQLSL
                     YIGVEEKDIPAALKIIESNCRSHLSIRTERGAGKFHRSVDSLQGLGGTVVFIWNIDQI
                     ELF"
     gene            complement(20289..>21331)
                     /locus_tag="PLL88_05420"
     CDS             complement(20289..>21331)
                     /locus_tag="PLL88_05420"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012236.2"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOJ01039.1"
                     /translation="SSIIFTGPPGTGKTTLALDAILSQRNSDVKCVYVAVAQKSTSII
                     STLQTLRAGGVLSKTVVIVSGPDDPPALRYLAPYAGVTIAEFFLDQGMDTLIVYDDLT
                     KHAESYRELSLLLRRPPGREAYPGDIFYLHARLLERACKLDPRHGGGSITALPIVTTQ
                     KGNISGYIPTNLISITDGQIMLDIDLFNKGIKPAIDIGRSVSRVGGSAQEPALKSLVG
                     PLKLELSQYEEVNRFARFGTDVNESTRRQIERGRRLESLLMQKPHDPVPFIDQVILFF
                     ALTNNYMDAVKVEDVPHFADELLSFVNMHESRLVDSIKHKRVLDDQMIENLHEVLSEF
                     EKLWLEKNRGGE"
BASE COUNT         5935 a         4869 c         4934 g         5593 t
ORIGIN      
        1 attgttaccc atatggtatt tttttttgga caaattcgta ttgcatttgc aatgttcagc
       61 aacaaaaaga ggctggctct aaaatttgag acagcctcta aattcttaat cgaaatcttc
      121 gccatttgag gcgataattt tcttatacca gtaaaaactt ttcttcttat acctcttcaa
      181 tgtgtcttcg acaatctgtg aaccaaacac gtatttcatg gaaaaaccat ttagcaactt
      241 ctcgattaat ttgtctgaat gattcccgct tttttcgcaa taaccagcgc accacgaacg
      301 ctgagcatat caatctcgtc tggctccgtg acgcactgca cagagcagtg agaatgaccc
      361 ggtatttgct gaagcaggta ttcatacact ctgcagcgct gaggtcgacc gatcaaaaca
      421 aaagattgat tggtctgccc caccaatctt tcgaaggaag gcaatgtgca caggtcttcc
      481 gcagcaatca aggattcgat gaggagacgg cgttcacgaa gcggagcctg gagcaaagta
      541 tccatgaaac gcaccagcag caagccgcgc aagaaacccg atgtctctat ttcacttttt
      601 gcaatctcgg caattcgtgt atcaaagaaa cccccatcct cctccatttc tgccgaaagg
      661 gattttccga taatggtctc tttcaataat gcatcataaa gctgacccga aagagtggta
      721 gcactgccaa ggatggtacg ctgcgcatcc actgcaatga atttagtgtg agaagacaac
      781 acgatcacac agcacggcac gggaaccttc gaattttcca gaagcccagc aatctgcgtt
      841 tcttccccgc gcatgaaatc caatgcgtct agatcttgca gttgaacaga cgctgggtca
      901 aaggcattct taatgccacg gatgaaatac actggaatgg acgcggggaa aatggtggaa
      961 tcgacataag gatgcaagtt accggcaagg gcatccatgc tgcaaggtgc ccacaaatgg
     1021 gggacttcct gcagaccgat ctccgaagag atcatcccgg aggaaaggat gcaatggata
     1081 tccgagatct ggaatttggt ctcgtataaa acttgttcaa agagcgaacg taatgcctct
     1141 ttaagggctt tattgctgcc tgaaattgcc gtatcacgca ctcccacccg ggcactggca
     1201 tgatacagca agcgggcatt tgaatccaca acggaaatac gactgttggt agtgccacaa
     1261 tcaatagtga taatgttcat tcgataaata tccaggcatt tagaatatga gtcaagttct
     1321 tcgtacgaaa cagtctacca gattattcgt tcaagccaaa atcaatcgga tattgttctt
     1381 tgagaaaaat tcgcaaaatt caggaggagg cattgagtcg gtaatgatgg tgttgatcga
     1441 cctcagatcg ccgatcctat tgggataaca ctttccgaat tttgaatgat ccgctacgat
     1501 gatcaggtta tcggaatgct ctatcataat tgagaccgtt aaagcagata attggctttt
     1561 aatggtcaaa ccttcttcaa tggtaagtcc agagacgcca atcactccag tgtcaaagta
     1621 attagacagc aatagctttt cagttatcgc tccatccaac gtaaaaaagt caccgtgaac
     1681 gtttccccct gtacatacca ctttgtaatt gatcttattc gccaactcgt agcacacttc
     1741 gattgaattt gtataaatgg tcaatttttt gtcatatctg gcaagcgctt tggcaacgaa
     1801 ccgaaccgtg ctgcccgatc cgatgatgac attgctgcct tcttttaaaa acttggctgc
     1861 tttaatgcca atgcggtttc tttctgagaa cagatccgaa tcttctttga aagtaagatc
     1921 atccttggca tggttcgtta tccgaatacc tccatgatat cgatcaatga tctgtctgtt
     1981 gtggagctcc gcaacatccc ttctgatcgt ccattcagaa acatccagtt ctttcgataa
     2041 ttctcgaatg gaaatggaat cattgatctc aagcaagtgc aaaatatgat cctgacggtt
     2101 gatagtacgt gaattcttca tttcatttat gcggatttgt gcatgaagct aagaaattgc
     2161 tcatttgggc ataattataa ttgatttgct caactcattc gattattctt tatagcatgc
     2221 gaacgaagat tggaaaacga aatttatcaa tacaataccg gaaataaatg acattatgga
     2281 ttcactaaaa aatgcccttc ctgccgaaca gatcaatttg aacgttagcg tcaatgattg
     2341 gcaggaagcg gttcgcgaag ccgggcaact tctattggaa gcgggcgcag tttcacccca
     2401 atatatcgat gcaatgatac aaaccgccaa agagctgggt ccctatattg tgatagccaa
     2461 aggcattgcc atgccccatg ctgccagtga agcaggagca aaaagagcag ctctatcact
     2521 ggtcaagcta gctcatccca ttgagttcgg caatccagat aatgacccgg tggagttggt
     2581 gatcggattg gcagctatcg atggagaaac gcacatgaac accatgcgta tgcttgcaga
     2641 gttgatcatc gatcgagaac gggtttcacg gttatttgca gcgcagacaa aagaagaaat
     2701 tttacaagtt attgcacagt gacaggaacg atgaatgctt gaaataaaga cattgacaga
     2761 agtggacgta ctggtagtag gcggaggtgc agcgggctgt ctggctgcaa ccgcagcagc
     2821 tcgcagaggg agaaaagtgc tgctagtcga agccatgagc gccttgggtg gaagccgcac
     2881 ctggatggga gtagattcgt tcaacggtat ttacagcccc ggaacaaaga gctaccaatt
     2941 ggtgggcggc atcagctttg agatcgccga aaagctttta acacagggcg gagcgtttct
     3001 acgacaaaat acctttggtt ccggcccggt tgttacgtat gaaattgaga agctgaagat
     3061 tctctatgaa gatttggtca tccggtccgg tggacaggta ctattttata ccaatgccgt
     3121 ggatgttgaa tgcaaagatg gcaaaatcga tagtgtgatc atttgcaata aaagcggttt
     3181 gagccgggtg aaagcccaga tcgtgatcga taccagcggt gatctggata ttggcgcaaa
     3241 agcaggggag gattatgaat tagcgggaga tcatgataat ccggtgcagt cgctgaccac
     3301 gatcttctac atggcgaatg tggacaacga aaaagcattc tcgattactc aagaagaacg
     3361 cacccgtatc atgcagcagg cgcatgaaag tgggaaatat acattaaccc gcatcggagg
     3421 atccattcat cccacgccca atccgggttt cgtgcatgcc aatttgaccc gcattcccaa
     3481 cataaacgca attgatccat tcgctatcag cgcggctgaa atcgaggggc gaaaacaggt
     3541 tcaagaatac gtacggtttt tgatcaatga gtatccggga tttgaaaacg catacctggc
     3601 atccactgcc tgcggtattg gaataagaga gactcgccga ttgaagggag agtatgtttt
     3661 gaccggcgat gatgtgcgca agggagcgaa atttgaagat gccgtggcat gttgttcggc
     3721 accgatagag gatcatgtag cgggaaaaag tgtcagatgg caatacctcg catcagacga
     3781 ttattatcag atcccttatc gatcactcct cccggtcaag aacaaaaact tgatcgtcgc
     3841 agggcgctgc ctgagcgcaa cccatgatgg gcaggcatca gcacgtaatt ctgcgcaatg
     3901 catggcaatg ggcgaagcag ccggtttggc tgccgcgttg tgcaatgaaa gcgggaaata
     3961 tcccaacgaa ctagatagcg gtttgttaaa gaaagaactg accgagcagg gagtgctgct
     4021 tgctccacag gaagtgaatt tgaaaggatt attgtaatga acgtgatcag aacggtatta
     4081 ggtgatattc cagcagatca attgggtttt acagacgctc acgagcatct catcatcgac
     4141 cgcgattatg tgctgacctt aaatccggat tatcggttgg atagcgttga aaagacctcg
     4201 aaagaagtga agtcctttct ggatgcagga ggaaggtctt tcgttgagat gacctgcctg
     4261 gcattcgggc gcagcgcaaa aaaactgatc gaggtatcga gaattacggg ggcgcacatc
     4321 gttgcttcca ctggttttca tcgacctaat ttttacatgg acagtcattg gcgttttctt
     4381 tattccgttg atcagatcgc cagaatcttg gtggaagaaa tcgaaatggg catggatgag
     4441 aatcaataca atggcccgat catcgaccgc accgatgcaa aagctggggt aatcaaaatt
     4501 gccacaggtt atttcaatat caaatctgaa gacttagtga caatggaggc agcggcaatt
     4561 gcccacaatg aaactggcgc acctatcatc acccatactg aaaatggcac tttgggttta
     4621 gagcaggtag aaacgcttgg atcgtatggt gttgcaccgc agaatatcat cgtggggcat
     4681 tacgaccgca atccagattt ctacctgcac caagaattgg ctcaaaccgg ttgttacctg
     4741 gaatacgaca cgcccagccg gcggaaatat ttcccagaag caaacttcat cgacctggtc
     4801 aggaagatgg tggatgccgg atttgaaaaa cagatcctgt ggggcggtga cttatctcgc
     4861 cgcagttatc aaactgcgta tggagggcag cccggattgg catatatatt gaataagttc
     4921 attccacgaa tgcgcgcaga aggttttagt gatgtcacga tcaacaatat attcatccac
     4981 aatccttccc atgcattctg tatcgcaaag aagaaataga agaaggagaa aatgtatggc
     5041 aaaaaagatg aagattgtaa cggtatgcgg ttgcgggttg ggcagcagtt tgatggcaaa
     5101 aacgtctatc gaaaagatca tgaaagaatt tggcgtcatg gcttcgatcg aggcgagtga
     5161 cgcaggcacc gccaaaggat atccatccga tttgatcgta acgaccacca tgctgaagcc
     5221 acgtgtgggt gaagttcccg gtactcccgt tgtagcagtg aagtctttta tgaacgtgga
     5281 tgagttgcgt gaagtgatgc aacctcacgt agaagagttc aagaaaaaga acaacatgta
     5341 attttcgttt tctttataga aaattcaata gttcatttca gaaaacctag ttctttttcg
     5401 aaagaaagga ggtgcttatt gaagtattgc caattgcaca cccatcttat tgatcgattt
     5461 acaaaaaaat agaattggag ataaagatga ctgtaataaa ttttttgatc aatgaagtct
     5521 ttgtagaagc accagtgttc ctgggcttag tagctctgat tggtttattg cttcagaaaa
     5581 aaacaggagc agaagttttg gaagggacca tcaagaccat tgtcggaatg atcatcctga
     5641 attccggtat tggtgttttc ttgggttcac ttagcccgtt aactacgtta ttgaacagtt
     5701 ccttcggaat cacgggtgtg ctgccagact gttttggtcc ttatggcgta gccatgcaga
     5761 cctacggcgt tgaaatccca atggcgtttg tgctggcatt tgtgttgcat ctgttattgg
     5821 tccgcatcct ccccttcaaa aacaccaaga atgttttcct gaccggtcac atcatgctct
     5881 tcaatgcttc catcatgctg ctggtaggac gctccgcttt aggattaacc ggcggcgccc
     5941 aaatcgcatt ctctgccgtc atgaccgcat tgttctggac attcatgccc gccatcgcac
     6001 gtccaatcac cgatccggtg accgataaac gattcaccct ggggcacctt cagactttgg
     6061 acctgcccgt tggaaagtgg ttcggtggtc tggcaaagtc aaaacgagct gacgaattag
     6121 aattacccgg catcttcaac ctgttttccg actataccgt tctcctgtgc gtattgatgc
     6181 cggtcatgta catcgtaatt ggtctaacgg ctggtaaagc tgcagtggac agcatttcag
     6241 atggcggaaa ctgggtcatc tggttaatca tgcagggcat tacctttgca gccggcatcg
     6301 ccatcgtact gtacggcgtg cgcatgttcc tggcagccat gatcccagcc ttcgagggca
     6361 tcagcaaaaa actgcttcct ggcacacagc ccgctttgga ttcaccggta tttttccagt
     6421 actcacctgc tggaagtatg ctggggttcg tctcctgctt tgttaccatg gtgatcttca
     6481 ccctggtaca ggttgcgatt ggtagcccgg tcgttatctt ccctggtccc attttcttct
     6541 tcttcgatgg tgccctggct ggagttttcg gcgatattca tggtggttgg gtaggtgcaa
     6601 tcatcgctgg tgcggtcatc gccattgtca tcaatgtcgc cgcactggta acctatccat
     6661 tccaggctga attctttgcc ggaaccggct tgatgttcgg cggtggcgac tacctgattt
     6721 ggtcaccaat cttctggctg ctcaaattca ttggtggcgc attgggtttg actgcataat
     6781 cattccattg tgctgacctc cgttttttaa tcatatgatc cggaaaataa actgatggtt
     6841 tcatccattt tccggatctt acacactgaa acacaagaaa agtgccgtaa ttggaatgga
     6901 aaagaatttt aggtcttgta acatagccat gaacatgatt gtaatgaatg ccactccaaa
     6961 gaatatcgct catcaggcaa tctacgattg ggtgaataat gagttgggca acggcacaat
     7021 tatggacatt ggcagtgaat taggttttgg tttgcaccgt cttgcctcca gcaatcgcct
     7081 tgtgatcggt ctagatatcc aactagacga acttactttt tccaaaacga gcaaacattt
     7141 tcataataag aattattgtc atatctgcgc cgatggcatg gtgatcccat tttctgataa
     7201 ttcgtgcaat ggtctatgca tgatcaacgt actgcatttg gttcacgatc tcgtcaaagt
     7261 acttgaagaa tgctggcgaa ttctctcccc gcaggggttg ctgatcgcca ccatcccgac
     7321 cgactacaac ctgccggatg aatggcgtat tccttcagaa aaggaatatt ttgaaagcat
     7381 cttgaaaagg gcatttccgt cagtagaatt tccacaagaa atcaattctc tcgaaaaaga
     7441 ttcagccctc tttcaaaaac aaaccgaatc cggcttattg acagcagttt gcagaaaaaa
     7501 tcttttctca tgaaagggta caaaatggca tactcgcatg atttaatgaa tcgttttatc
     7561 catagtggaa tcatggcaat tttaagagcg gatgatccaa agaacatcat tcctgccctt
     7621 gaagcgctcg tcgaaagtgg aattgacaca gtagaaattt cgttggttac ccccgatgca
     7681 gtaaagatca tccaagagct acacaagtcg ctgggttcaa agatcgtgtt aggtgctggc
     7741 actgtactcg acgcagagtc agcgcgggca gccattctcg caggggctga atttgttatc
     7801 tcccctgttc tgtcgctgga tgttttgaag atctccaaac gatatggaaa gatggctttc
     7861 gcaggagcat ttaccccaac cgaagcattg accgcctggg aaaacggaag cgatgcggtc
     7921 aagatattcc ctgccatgcc tgccggtccg gaatacatca aagctttgca tgccccattg
     7981 ccgcagatcc cattgattcc ggtaggtggg gtaaccctgg agaacatgga agcgttcatg
     8041 gatgccggtt gtatcgccgt cgctgcaagc agcagtctgg tcagcaatga cttgatcaaa
     8101 gaaggcaagt tggatgagat caaacacaat gcaaaacttt ggatctcaga aatgcagcgc
     8161 atcagggaaa agaaatgatg cagaaaggaa cagcatgaaa ttaaaaggaa tcatccctcc
     8221 gattctgacg tcttttgatg aaaagggaaa aatcgatttt aaaagattag aaaaatttgc
     8281 cgaattcctg cgtccgcatg ttcaaggttt ttatgtgaat ggtacctacg gatcaggcgt
     8341 tttaatgaac gtagccgaaa gaaaaagcgt tttcgaagct ttgtcttcct attctgacgg
     8401 aagcttcgaa ttgatcgcac atgtcggaac caccaacctg cgcgatagca tcgaattagc
     8461 acaacatgca gagatacacc gggcattagc cgtagcggct gtacccccct tctatttccg
     8521 atattcagaa gaaagtttat tcgcattctt caaagatctg atccagtcgg ttgccgttcc
     8581 tgtctatcta tatgacaacc cgggtgcaac cggaaacccg ctttctgccg atttgatcaa
     8641 tcggttggca gcggtcggtt tggcgggtgt taaagacagt acatttgaca tcagcaaaac
     8701 ctatatggtc atgcgcacga tcaaaaagga aggatttgat gtggtgatcg gttctgaaag
     8761 tcttttcttg ccggcataca tgcaaggttc acgcgcatgc atctccggtc tggcaaacac
     8821 catgccagaa ctaatgcaga agttgttctg tgcagcagaa agcaatgatt tccttcaagc
     8881 aacgaccctt caacggcagg tactggaaat gtgggatatc ctgcactatg gtccttccac
     8941 ggcaactgca tacgccatgc tgaaagtcag ggggatggat gcaggttttc cacgcagacc
     9001 ctttttacca atcggaaagg atctatacga aaaaattgaa tctgccatga aagctaatga
     9061 agcgatttgg aagatttagc ttttacaaaa atgccagcat agccatgatc atgaaaccag
     9121 acctcacaaa tcacccaatc aaagctgttt tgttcgatat ggatggcgta ctggttgatt
     9181 cgatccctat acacattcaa tcctggaatg aggttctcct tcagaaagga ttaccagagt
     9241 attcaacgga tctctacttt tctgcgttgg gacgaacaag tttggatatg gtgcgcacct
     9301 attgcagaga aaaccagata atgctcacag aatcgcaaat gcaagatatc ttaagtcaaa
     9361 aagaagacat ctttcaaaag aacatcggtg agtatgcatc cacgacccct ggagtgatcg
     9421 actggttgga ttattgtaaa tgcctgggca tcctttgcgc agtggtctca tcatcaacaa
     9481 tgaccaatat cattcacgtc ctccattctc tgcacattgc tgattttttc agcgccatcg
     9541 tgtctggagc aaattttcca gccagcaagc ccgacccacg tgtttatcaa aatgcggcgg
     9601 cttccctggg gatgccagcg caattctgct tggtcgtgga agatacaccc accgggatcc
     9661 aggcagccaa gcatgcaggg atggtctgct gcgcagttac cacttcatat ccaaaacatt
     9721 tccttgagaa tgccgacctc gtgctcaatt ttttatccga tgcagaccca aaaatcttat
     9781 ttctgaatga gagaaggaaa aaatgaacgc tatcttattg aggtttatga tcgtcatctt
     9841 cagtctggtg ttgctgatct ttctatttgc ccgctttgca ccaaaggcat ggagcaaacg
     9901 aatttatgga acgatcgttt ggctgattga cactgcattt atctattttg tcaatttttt
     9961 catctttttt aaaccttgga aagaacacaa ggaaaagata gggatttatg tatggttgtt
    10021 tctggtattc ataatcgtcc gatatttatg cgcagaacaa ccactgacca ccgagatcaa
    10081 atccccctat ttagcattca ttggttacat ctatttcatc agtttccttt atatgatcaa
    10141 gttcattttt gagtttgtca taaaagacat caaaactacg gattgacact ttattaaaaa
    10201 gagaggattt tgcaatgaaa aaaatcgaca agagaatcca ggacatcttg gagaaaggtg
    10261 agaaggtatt agtttctggt gttccagtgg gttaccccaa cctcgaagaa actcgaaaac
    10321 ttgttgaagt gtacattcgc tcaggaatgg atatcgtaga attttcaatg cctacgcgaa
    10381 atccatatat tgacactcaa atcatcgcgg attcgaatgt gaaagcattg gatcttgaac
    10441 ctgatttaaa tcattatttt gaaaccttac acatcatccg taaagatttc cccaatgaac
    10501 cattctatat gatggcgtat gctgatctga tcacacaatt tggtctgaag aattttgtag
    10561 atcagttagt agaattggag atcgatggcg ttgaattacc tgataaagat gaaatggtac
    10621 cagaattggc ttctgaaatg gaagcattgt tcgatcaaag tggcatctac cgaatttata
    10681 tcctccatca tccctataat gaggcttttt tcaacagaat taaagaaaaa acggctggtt
    10741 ttttgctgct acagtccatt gctgatgaag aagggaatcg tccaaatgtc aatccagaaa
    10801 ataaggattt gattgcaaaa atcaagaaag aaacggatgt tcccgtagta ttaggttatg
    10861 gcataaaaaa taccgatcgg gtaagagagg ctgtttcact cggagcagat ggcgttttgg
    10921 tgggcacttc catggtggaa tatatcagtc agggcgacta tcaaaagttc tcgcaattca
    10981 tccgaagtct aaaagaggct actcgcaaca cctaaaaaca aatccgtatg ctcaaaaaac
    11041 tcaaggacaa tctggttgat ttcgattgtc cttgttttat aaatttcatc gatgtttatc
    11101 cagcacccat agaaaaacac acgcaataat ctgttcctcg gctatggggg atttttttga
    11161 aatctattgc tcaatggttt tccatctcac aaccataata aacatatacc atgagagaag
    11221 ggcagtttcc tcttgacaaa aatttttgaa tccatataat gtaacccatg cgtagccaaa
    11281 ttggtaaatc actgaattgt atgtgtacat gtatgatgat gcgtaggcga tggtgttcgt
    11341 catgcatgtt ggtgcacgtc tgtgactaac caatagtgca cgagcccatc gcttacccat
    11401 tttgaaaagg ataaacggtg ggcagacaag aaagtcaatt tcgctaataa aaatgccacc
    11461 aattatcctg gtggcatttt gttattaatt cagaattctg tgtataaaaa aatctttgga
    11521 ggaaaaaatg agaacaacag taagtcgtaa acagtcaaac aaacccataa aatttacggt
    11581 cattgtgacc gccttagttt tatcgattat gcttgtttca gcctgcacag ccacggccgc
    11641 agaggctacc gaagccgtta ccgccgacgt ttctacggaa agcacaagcg cggcagagcc
    11701 agccaaagag ccgatcaaga ttaaaatcgg cgtctcccct gttccgcacg gtgacatcat
    11761 caccttcatc aaagagaacc ttgccgaatc cgccggtttg gacatcgaga tcatcgaatt
    11821 cacagattat gtgcagccga atttggcact ggcagatgaa gaaattgacg caaactattt
    11881 ccaacacgtg ccctacctgg acgatttcaa ccaggagcat agcaccgatc ttgtagcggt
    11941 ggcagcagtc cacattgaac cccttggtat ctactccaag acgatcacct cgctggatga
    12001 gatttctgat ggagcggtgg ttgcgattcc taacgatgcc acaaacgcag gacgcgcctt
    12061 gaatttactg gcagccaatg gcttgctgac cctcaaggaa gatgtgggct attccgcgac
    12121 cgtaaatgac atcgtagaaa atcccaagaa ccttcaaatc agagaacttg aagctgcaca
    12181 attagtgcgc tcgctggatg acacgacgat ttcggtgatc aacggaaact atgcccttga
    12241 gggcggattg acacctgcaa ccgacgcttt ggcactggaa agtggagaga ataaccctta
    12301 tgctaatgtt ctagttgtaa aaagcggaca tgagaatgac gaagggattc gcatcttgag
    12361 cgagttgttg acttctgctg aagtcaagca attcatccaa gataactaca acggttccgt
    12421 catcccagca ttttaggata ctcacccgtt agaaaagtat gcattcgagg aagatgattt
    12481 tcgaatcatg tatcaaacac agaaactgac tggattagga tgaaaatccg gaaaaacctt
    12541 ttcaaaatga actccatctt acaagaagga gtgaataatg aactccagga gagaaaaacc
    12601 ctctcctgga gttccattaa ccctgcaaag gcaaagcgca atatgattct cattagaaat
    12661 ttaagcaaga tttatgaaca aaacggacag tccattcaag cattggatga cgtttcttta
    12721 tctatccccc ggggtgaaat attcggagtg gttggtccca gcggcgccgg aaaaagcaca
    12781 ctgatccgct gtgtaaattt acttgaaaag cccacgtctg ggcaaatcgt gatcgatggg
    12841 caagatttga caaaactctc gaattctgag ctgcgtcaag cacgtagcca gatcggtatg
    12901 atatttcaac acttcaacct gctcagttcc cgaaccgtgg ctgggaatgt cctttttcct
    12961 ctggaaatca taggatacga aaaaagccag cgcacagcca gggttgaaga attgctcgag
    13021 ctggtggggt tgagtgataa gatcaacgct tatcctgccc agctttccgg gggacaaaaa
    13081 caacgcgtgg gcatcgctcg cgctcttgcc acagaacctc gtattttact gtcagatgaa
    13141 gctacttctg ccctggaccc gcaaacgaca acttctatcc tgaacttatt gaaagatctg
    13201 aagcgccgtc ttggcttgac cattctattg atcacccatg aaatggaggt agtcaggcaa
    13261 atctgtgacc atgtggcggt tcttcaggat gccagaatcg ttgaacaggg gtccatcgaa
    13321 gaactcgtca tgaaaccgca cagtcttctc tcgcaggctt ttttcccacc cgttgaaaaa
    13381 gtcatttccg tgcccgacaa aaccattatc accattttgt tcatagggaa attggctgac
    13441 cagccagttt tatccaagct ggtaaaagct tttgacgtcg aagtgaatat tcttggaggc
    13501 agtattcaaa atatcggcgg aaaacgaatg ggacatctgc aaattgaaat ttccggcaaa
    13561 gaagtggaac aggcattgaa ttatctgcaa gaactgggat tgcatgtgga ggtacaataa
    13621 cgaatggatt ggtcaatctg gttattcaac ttatcaaagg ggacagttga aacgatctat
    13681 atggttggga tttcgacggt ctttaccgca ctgtttgggc ttcccttggg aattttattg
    13741 gtgatcaccg atcggaacgg tctcttgcca tcaccaactg tcagcgatat tcttggagtg
    13801 atcgtaaata ttagccgttc cttgccgttc atcattcttc tcgtggttgt cattccgatc
    13861 acccgctttg tggttggaac aaccatcggg gcgacagccg cgattgtgcc gctcacatta
    13921 gcagcgatac ctttttctgc cagggtggca gaaagcggtt tacgcgaggt cgatcgtggc
    13981 ttggtagaag cagcgcaggc gatgggatgt tccgtctggc aaattatcac caaggtgctg
    14041 attcccgaag cattaccctc tttgattcta gggatcactt taacgattat cagcctgatc
    14101 agttactctg ccatggctgg agccattggc ggcggtggtc tgggcgattt agccatccgg
    14161 tatggttatc agcgttttga gactgagatc atgatcgcta cagtaatatt gctgatcatc
    14221 cttgtgcaag gcatccaggt gctgggtaat cacctggcaa aaaaggtttt gaaaaggtaa
    14281 atgattctgg taagaaaatc aggaagaaat agaagagtta accaatggag gttatttaaa
    14341 atggcaaaat cccaaaaatt tgaaacgctg gcaattcatg cagggcagcc ccctgatcct
    14401 acaacggcag ccgtaatcac ccctatctac caaacttcca cttttgccca ggagcaggtg
    14461 ggagtcacca aagggtttga ttacggtcga actgcaaatc ccacccgact tgcattagag
    14521 caatgcctgg ctgctttgga aagccaaaaa cgcgggcagg cagcttacgg gctggcgtat
    14581 tcttcgggca tggctgcaat cgacaatctg ttacggttga tcaagcccgg cgagcaagta
    14641 atcgcttcga atgatattta cgggggaacc tatcgattat ttgaacgggt tctgaaggaa
    14701 tacggaattc aattcactta tacagacact tgcgatctgc aaaaaatagc caactgcatc
    14761 caacctgaaa cccgtttgat ctggttggaa tctcccacaa accccatgtt gaaaattgcc
    14821 gatatcgctg ggatctcaaa gcttgtgaag agccatccca atcgaatccg aattgcggtt
    14881 gataacacgt ttgcctctcc ctacctgcag cagcctttaa tgctaggagc cgattttgtc
    14941 gtgtattcga cgacaaaata tctcagcggt cattccgatg tgatcggtgg ggcgatcatt
    15001 tcccaagata aagatgatta cgacaaatta aaattcttgc agaatgccat tggcgcaatt
    15061 cccggtccgt tcgattgttg gttgactttg cgcggaataa aaacactggg aattcggatg
    15121 gagcggcatt ctgaaaacgc aaagtacatt gcagaatttt tagagaagca tcccaaagtt
    15181 gaacgcacct tgtaccctgg gttgcccaat catgcagggt atgaggtggc gaaaaaacaa
    15241 atgcgctcgt ttggcggaat gctttccatc atattgaagg atggtgccga agctgccaga
    15301 agaatggtgg aaaaaaccca aattttcaca ctggctgaga gccttggagg agttgaatca
    15361 ttgattgaaa tacccgctgc catgacgcac gcttcggtcg caggaagccc gcttgaagtt
    15421 cccgccgggt tggtacgttt atctgttgga attgaagcaa tcgacgacct gctcgaagac
    15481 ctggatcagg cgttgcagta tgcctgacca gctacaacgt agtgacgcaa aaaagcaggc
    15541 actttttaca acgtctgtct cgaagtactt gactcttaat cacccttatg ggtgagatat
    15601 tcgatcagat cgattttggt tatcattcca acaggttgat tggtaacatt aacgacaacc
    15661 acaacctcag actttttgaa aatcggaagg atgttctcga tactctcatc gatgcttgca
    15721 gtcggaactt tcggattgac aattttcgca atgctttcca acggatcacg ctcgtggctg
    15781 gcatgcagca ggtgatccag aacatcggct tcagcaacca gcccgaccaa acgaccatcc
    15841 tcgtcaacca ctggaagttg ggaaatatca taacgattca ttttatccac aaccgctctt
    15901 atctcttcat caggtcgtgt aaccaccagc ggtattttgt tgctggtttc cagcagcaag
    15961 gcaatggaat cagtaatctt cgccaccggc atgaagccgt tttcgctcat ccaattgtca
    16021 tcgaaaatct ttgacaggta acgattccca gaatccggga ggatcaccac aacattttga
    16081 tcagttttga atgaacggac gatcttcgac tttaacaacc cggctactgc agatcctgaa
    16141 gaaccgccgg caaagatccc ctcttcgcga accaaacgac gcgtcatctg gaacgactcg
    16201 cggtcatcca cctgtaccac ttcatcaatc agggaaaggt caagagtgga tggcagaaaa
    16261 tcctcaccga taccctcaat tttatacgtt ttgggatgcg gatcggtcgg cactctgccc
    16321 aatttccacg tgtcataaag caacgaaccc aaaatatcga cggcaacgat tttaacatcc
    16381 gggttctgtt cttttaaaaa tcgtgcaatc ccgctgatcg tccctccggt gcccatgcca
    16441 gcaacgagcg catcaatctt tccgtccatc tgtccccaga tttcaggtcc ggtgaaatga
    16501 taatgcgcat caggatttgc cggattatga tactgatttg ccaaaatact gttgggcgtc
    16561 tcgcgtacaa tgcgagcagc cacgttgtaa taactgcgag gatcttcagg agaaacagcc
    16621 gtgggggtaa tgaccactcg cgctccaaac gcgcgaagaa aacgtacttt ttcatcgctc
    16681 attttatcgg gcatgacaaa aacgcatcta taccccttca ccgaagctac aagagccaag
    16741 ccggcgcctg tattgccgga ggtcgcttcg acaatagttc caccaggcag caagcgacct
    16801 tccctttcgg catcctctat aatggataaa ccgattcgat ctttaatgga accagccggg
    16861 ttaaaatatt ctgctttggc atatacagcc gcttcgattt tctcagagac aaccttattt
    16921 aaatggatca gcggtgtatg cccgatggtt tccaaaatat ttccgacgac ctcaggtcgt
    16981 ttttcttgca gcatggtcat ttgattcatt ccctgtttgt tcattcttac tctcttttta
    17041 atatagaaaa gaccaaccaa aacaaaaaac ggcagacaaa tccaccacaa cataaatctg
    17101 atttatgcgg atgactgtct gccgtctgtg acggttggtc tttctttcag ttattcatca
    17161 catggcggaa gaagcagtct ccgccatcga cacatgcaac gcacagtttt ttcctctgaa
    17221 tttatattat tattttgaag tatatcatgg atattacgaa ttgggaatag ccaccatttg
    17281 ggaacagtca tcccgtatac ttcatcggta tagtacagta gaaattttcc cgaccctgta
    17341 gatagaggac tggtgcactt gacacacctg gcgtgcgatc aaggttaccg aaaactgaga
    17401 acggcggtct ccaatgcaag gagcatcata tcgttaaacg cattctggcg agcttcggct
    17461 gaaaggttct ctccctttcg gaaaggacta tccgataccg tcagcaggca ggcggcttct
    17521 ttctttactg cgcgcgcatt agcaaacagc gcaaaagatt ccatctcaga agcgatgact
    17581 ttgttttctt tataaaccgg ctgccaatcg atatcgaccg tggtataaaa aacattgtca
    17641 gaaacgacat tcccctcgtg caccttgatc cctttttgat ctgctacctg cctgacgatc
    17701 ttcgagatcc ttgggctgcc cttaatgacc ttgtcttcga tgccatgcgc gtctctggca
    17761 aaggtctcag ttgaaaatga cccatccacc aggatgatat ccatgatatt caattccggc
    17821 tgccaggcac cgcaagagcc aatgcggatg atggtttgga catcatagaa ctggtacagt
    17881 tcgtaggaat aaatgccaat actgccgaag cccataccgg aacccatgac gctgaggcgc
    17941 tttcctttgt acattccggt aaatccgagc atgccacgca cctggttgaa ctgcttcaca
    18001 tcgtccaaat aatcttccgc gatctttttc gcccgcaggg gatcgccggg catgagcact
    18061 actttggcaa tctcatctgt tgaaactgct tcaatgtggg gagtaggaat ggtcggcata
    18121 cctcgtcctc ttttaaaaaa tagtaaattt tgtatggata aacaataggt atattctata
    18181 acaagaaagt tccaacaagg catcatcttc catacaaaca tccattttta ccggacactc
    18241 gtaacaatca tccattaata aagtagggga aacaaagtcg aaggtaattg attcagcgtc
    18301 agttccaaac gactgtccta taatcgtgca cagtttatgc tctaaccact ctcaaggaag
    18361 gctgcaaaag caacccaaga tctttgaaat ttcatggcta gacaatggaa aaacagtcta
    18421 tgaaaaacaa gattggtaag atcatcagta ttatcagtgt tgctcctttg atcgctttgt
    18481 atgtgttaac cgctcttttt ttgcatgatg caaacttatt cggtaataat gcattgtggg
    18541 ttggaatttc catcttcttt ctcacattga tgcccttatc cgcttatctc ctcgaacacg
    18601 tactgcctgc ctttaagaca gcagggagaa aaggtgaaag aaagctggca tttgcgatgt
    18661 gcatcgccgg atatgttctg ggaacgatcg tgagttttgt gttccatgcc ccgctggcgg
    18721 tgaaaatggt gttcacttca tacatggttt caggcggcat tctcgcgctg gtgaacggtc
    18781 tgatcaagtt caaagccagc ggtcacgcct gcggcgtggc aggcccatgc gtggtgctgt
    18841 tatattttat gggagcaaag gtctggtata tcatcctcac cctggcgccg gttttctggg
    18901 cacgtattac catggggcgc cattccctta aagagttaat ctcaggcgca atgattggaa
    18961 tgggagctac aggactaacc atcgccgcat ttctgtttgt gagcaaactg atcgcttagg
    19021 cgatctattc aaataatttg acagaaaaaa ggtgcttcaa agcccgatag gatctcgtct
    19081 tcacgagaaa gttttaacga agcagacctg cccccaccgc cagctcctga acctggcggg
    19141 tgatcgccag gcgcctttgt gtttgaacaa ccatggtcaa ttcttcgatc aggtcctgcg
    19201 tgttttcttt ggcattgtcc aaaaggcgga agcgggtgac attttcagca acgatggatt
    19261 caaggataat gtcatagaag ttaattgccg tgaggtactc aatcgttcgc cgggctattt
    19321 ctacggggtc gccttcgata atggggggag gccatgcggg cgcataatca tgcacctctt
    19381 caaagtgctt ggagatgggg agtaaaggag tgacaagtgg ctgataattg cctttgggag
    19441 tctggcgaaa agacagaata tctaccgcat tcaaactccc ctcttgatac gcctggagcc
    19501 aggagtagat gaaccgatgg gcaagatcat agcgcgggag tgacccgctg ccaagggcag
    19561 aatagtgctc aaactggaga tcaagctgtt gaagcgcctg ctgaattttc gtgccgaatg
    19621 cgagaatgcg tttctctccg gatttcgtgt tgaaccaatc aatggtcccg gctgctaaat
    19681 tcttattgta aggaccacac aacccgcgat cgctgcccaa gaccaccagg atagacttgt
    19741 tcggatccct ctctacaggc aggggaacag aaagctcaat gacagacgac gaagtcgacg
    19801 acaaagatgc caggattttg aaatattcct gcttgtaatc ctgcaacatg gtcttcttat
    19861 tgagcgccat gagcatggtg ctctgggaaa gaatgcgcag ggaagatatc agcggttcaa
    19921 ttgccctgag attgtccaag cggttttggg ttctttcaat tgcttctgac attattagaa
    19981 caattcaatt tgatcgatat tccaaatgaa aacaaccgtg ccgcccaacc cctgcaagct
    20041 atcgacagat ctgtgaaatt tgccagcacc ccgctctgtt ctaatggaca aatgcgacct
    20101 gcaattcgat tcaatgatct tcaacgctgc ggggatatcc ttttcttcaa cgccaatgta
    20161 aagcgataac tggctctgct tgagcaaacc accacgggtt tcgctatagg taaccttata
    20221 ccccgcattc acaagctcat cgagaacaat cttgccactg ccatgaggga tgacagccat
    20281 aatcatcttc attcgccacc tctatttttc tccaaccaga gtttttcaaa ttcactcaga
    20341 acttcatgca aattctcaat catttgatca tccagcaccc gtttgtgttt aatgctatcc
    20401 accaggcggg attcgtgcat atttacaaaa gaaagcaatt catcggcaaa atgcggaacg
    20461 tcttcaacct tcaccgcatc catgtaattg tttgtcaagg caaaaaagag aatgacctgg
    20521 tctatgaatg gaacggggtc gtgtggtttt tgcatcaaaa ggctttcaag acgtctgccg
    20581 cgttcaattt gccgccgggt tgattcatta acatccgtac cgaagcgtgc aaagcgattc
    20641 acttcttcat attgcgacaa ttccagttta agcggtccaa ccagcgattt cagcgcaggc
    20701 tcctgtgctg aaccacctac gcgcgaaacc gaacgaccga tatcgatagc cggtttgatc
    20761 cctttgttaa agagatctat atccagcatg atctgtccat cggtgatcga aatcaaattg
    20821 gtaggaatat aaccagagat gttgcccttc tgggtagtga cgatgggtaa agcggtaatg
    20881 gaaccgccgc catgtcgcgg atccaatttg cacgcgcgtt caagaaggcg ggcgtgcagg
    20941 taaaagatat cacccggata cgcttcacga ccgggggggc ggcgcaagag caggctcaat
    21001 tcgcgatagg attcagcatg ttttgtaaga tcatcataca caatcaaagt atccatgcct
    21061 tgatcaagga agaattccgc aatcgtcaca cccgcgtagg gagcgagata gcgcaaggcg
    21121 ggcggatcat caggaccaga aacgataacg accgttttcg atagaacacc accagcacgc
    21181 agcgtctgaa gggttgaaat gatcgaggta cttttctggg caaccgcaac gtaaacgcat
    21241 ttaacatcgc tgttccgctg gctcaatatc gcatccagtg ccagggtggt cttgcccgtt
    21301 ccgggggggc cggtaaagat gatggaagaa a
//
LOCUS       DAOSDS010000032        21259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1073_length_21259_cov_4.712554, whole genome shotgun sequence.
ACCESSION   DAOSDS010000032 DAOSDS010000000
VERSION     DAOSDS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 21259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21259
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1073_length_21259_cov_4.712554"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(348..956)
                     /locus_tag="PLL88_05425"
     CDS             complement(348..956)
                     /locus_tag="PLL88_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01040.1"
                     /translation="MKKKSNPLRHLAERISEYNEIAQIGEIARRYFAMNAFDGVLTIM
                     GVLMGHYAAGVEDPMIVVTTGLTTSVAIGISGLWGSFLTESAERKREMDELSYSTLTD
                     LNGSKIGKASRFAAVAVSVVDGLSPFLASLVVLVPFFFASFFSDIRMVYYTAIGFALL
                     VLFGLGMFLGRVANQNLMKMGLKTLTAGLVSILISNLLGVSH"
     gene            complement(975..1265)
                     /locus_tag="PLL88_05430"
     CDS             complement(975..1265)
                     /locus_tag="PLL88_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF211 domain-containing protein"
                     /protein_id="HOJ01041.1"
                     /translation="MIGKIRRLVLDVLKPIDPSIVDLAKKISVLDGISAVNISIAEID
                     HKVENAKITIEGDDLDYDVVAAMIRDQGGSIHSVDEVVAGKMIIDDAATLQD"
     gene            complement(1357..2436)
                     /locus_tag="PLL88_05435"
     CDS             complement(1357..2436)
                     /locus_tag="PLL88_05435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ01042.1"
                     /translation="MQKEKQDITTYFNISFEMGLKQIVRFSLNNPRLAYSAAKLYLAY
                     RLAEKKRAAFLKQGVQVPPMVIFSITNDCNLDCIGCYAKILHQTSAEELTPQQFRSVI
                     QQARDLGVSVILLAGGEPLMRKELLDTIKEFPSLIFLLFTNGTLLDSAAIQNIKKQRN
                     ILPVISIEGDQQDTDLRRGGGIYERAMQIFSKLKQEKMLFGTSITQTSRNFDLVNDET
                     YLTDMMEKGCKVFFFINYLPINPQTHDLALTPQQVMSHLAILEELREKHSALFMAFPG
                     GEVELGGCLAGGKGLIHINPQGDLQPCPFSPFSDVNLREMSLLQALRSRHLQVIRNSG
                     NRLDESDGACALWKNKSWVEELYKN"
     gene            2557..2703
                     /locus_tag="PLL88_05440"
     CDS             2557..2703
                     /locus_tag="PLL88_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01043.1"
                     /translation="MESGRKLCPQVILEGARRGMLQTAAPMDCKPVRDDPRPFNVPSS
                     AKSF"
     gene            complement(2789..4102)
                     /locus_tag="PLL88_05445"
     CDS             complement(2789..4102)
                     /locus_tag="PLL88_05445"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOJ01044.1"
                     /translation="MSTICVIGTGYVGLVTGVCFADLGNTVHCLDINAERIANIKKGI
                     MPIYEPGLEEVVERNVAANRLFFTTEYTEALKDAEFAFIAVGTPSGVDGEADLTYVRD
                     AAEKIAEVIDHPIIIVNKSTVPVGTGDWVADIIRKKRGQNTLEYYVVSNPEFLREGSA
                     IHDFMQPDRVVLGSVHRKAAEAVAELYQPLRAPALITDLRTAEMIKYASNAFLATRIS
                     FINEIANICDELGADIREVARGMGLDKRIGHAFLDAGLGWGGSCFPKDVKALAHMAVL
                     HGTHPQLLQAVMDINRNQRRKVVMKLRNLVGGSLVDKNIGILGLSFKPNTDDIREAPA
                     IEIIHLLRNEGAHVRAYDPQAMENTAKAIPDLQLCKNPYETAENADGLILVTEWNEFK
                     QLDFRRLHSIMRTPVLIDGRNQWDSKFLRELGFIYFGIGQGNHVN"
     gene            4209..5084
                     /gene="folD"
                     /locus_tag="PLL88_05450"
     CDS             4209..5084
                     /gene="folD"
                     /locus_tag="PLL88_05450"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOJ01045.1"
                     /translation="MTAAMIDGKQIAKDMRSRLTEQVAERKRLGKAVPGLATVLVGED
                     PASQVYVRSKHKACEQLGITSFSHVLPASTPQEELVELVDSLNGDPKVHGILVQLPLP
                     AGFDEEEVLLKVSVKKDVDGFHPINIGKLAQKGRTPSFIPCTPAGVIELLRSTGISME
                     GKSAVVLGRSNIVGMPVALLLVKENATVTICHSRSHNLPDLTRGADILVACVGKAEMV
                     RGDWVKPGAVVIDVGINRVDDPSAEKGHRLVGDVAFDEVREVASAITPVPGGVGPMTI
                     AMLMQNTLRAAELAD"
     gene            5190..6578
                     /locus_tag="PLL88_05455"
     CDS             5190..6578
                     /locus_tag="PLL88_05455"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HOJ01046.1"
                     /translation="MATLLVKNIHTLVTMDPARREIRNGGMFIKDGFIQQVGGSDEVT
                     ADADEVYDLKGHIVFPGFINTHHHFYQTLTRVVPAAQDANLFNWLKTLYPIWARMTSE
                     DIYISTKTALAELALSGCTTASDHLYLFPNGSSLDDEIRAAQEMGMRIHASRGSMSLG
                     ESRGGLPPDSVVDDEGFILKDSQRLIEKYHDPKPGSRVQIVLAPCSPFSVTGDLMRES
                     ASLARQYGVHLHTHLAETEDEQQFTVQQFGLRPLAYMQSLEWVGEDVWFAHSVYIDEE
                     EQKVFAATGCGVAHCPTSNMRLASGIAPLREYIRLGVKVGLGVDGSASNDGSHMLEEV
                     RQAMLLARVRAGLEGASLSQSGTSPIMTARKALELATLGGARVLGRQDIGSLEVGKCA
                     DFTAIDLNHISYAGGLHDPVAAVVFCHPRNVDYTFVHGKAVVEKGQLLSMDLVEHVQK
                     HNKAAARLLMEE"
     gene            6595..7323
                     /locus_tag="PLL88_05460"
     CDS             6595..7323
                     /locus_tag="PLL88_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ01047.1"
                     /translation="MQNISPYQRLQTELEQIINAKGAGAKLPSEPLLAKNLGVSRSTL
                     REAMRTFEVQGLIRRRQGQGTFVVGKQKTFEGGLEVLESIETLSQRIGLNVSMGELQV
                     KRYRVEKENQSYVNFPLKAAVIQIDRTIIAEERPIAYLIDIIPVAVLDENDLQQGFDG
                     SVLDWLLKRGDPVLSQSHARIDSIAASSEIAHKLQVQRAAPLLLMESDVYDISGQLVT
                     HSYSYFIPGFFDFHIVRKVANKEF"
     gene            7341..8294
                     /gene="pyrB"
                     /locus_tag="PLL88_05465"
     CDS             7341..8294
                     /gene="pyrB"
                     /locus_tag="PLL88_05465"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOJ01048.1"
                     /translation="MRSFLGRDILSLKDFERAEFFRLFEVALEMDEIARNRRNSDILK
                     DKTLVTAFYQPSTRTRLAHEAAMHRLGGHVTGFSDAKMTRAGDWYQESIKDTVHMLEY
                     YGDAIVMRHFQQGAPAEAAKWASVPIINAGDGWGEHPTQVLTDLYTVWNELGTIDGLT
                     FLCIGDMRMRTMHSISYALTQFDTEVYYVCPPEMSLTPEFKKELDEKNLVYYEADHVA
                     DVIDKADVIYMEPVVQPDYTKARQEASEDKGLTPDNYKVTRQLLKEKAKPDSIILHSL
                     PRMDELPPDVDGTRHARYWEEAFNGVVMRMSILASVLGATE"
     gene            8351..9331
                     /locus_tag="PLL88_05470"
     CDS             8351..9331
                     /locus_tag="PLL88_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOJ01049.1"
                     /translation="MQTNLHGRDLIGDLDFTKEEVETVLDVAWDLKRKRALNEPHAYL
                     RDKVLAMLFFFSSTRTRGSFEAGMAQLGGHAAFIESKTTQIAHGDTEKEIGDIFGRYF
                     DGIAIRHVEWGTGNRYLNLVAQASRVPVLNMQCEIYHPFQCLADLMTIMEKKGRDLRK
                     KKMVVSWAYAESYSKPISVPQSLILQMPRFGLDVTLAHPPEFVLMPEIMEQAKAEAKK
                     YGTNFEIVHDMDEGFKDADIVYAKSWGPLVYTTDAEKGAEITKKYSGWITNESRMALA
                     QEDAIYMHPLPADRNIEVTDGVIDGPHSVVFDEAENRLHAQKAVMALTMG"
     gene            9339..10295
                     /gene="arcC"
                     /locus_tag="PLL88_05475"
     CDS             9339..10295
                     /gene="arcC"
                     /locus_tag="PLL88_05475"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOJ01050.1"
                     /translation="MKKQVAVVAIGGNSLIKDNKNVTVESQYEAAKETTYHIADMMEA
                     GWDVAIGHGNGPQVGFILRRSEIAHKTAGMHEVPLDVCGADSQGAIGYALQQTLQNEL
                     YRRGIKKPVATVITQVLVDKNDKAFVNPTKPIGGFMDEAEAKQKEKELGWNVVEDAGR
                     GWRRVVASPIPQEVVELDAVKDLIEAGVIVITVGGGGIPVVDAGNRQLVGTAAVIDKD
                     YASSLLAREIKADLFLISTAVEKVSLDFGKPNQRQVDHMTLSEVKQYLAEGTHFAKGS
                     MAPKIQAIIWYLEAGGKKAIVTNPENIGRALRGETGTVIEPD"
     gene            10472..11713
                     /locus_tag="PLL88_05480"
     CDS             10472..11713
                     /locus_tag="PLL88_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ01051.1"
                     /translation="MISNKMKAIFALLIAASLLFSACAKASEPEEVAEPVAAEESEPF
                     VFGILMVGAYNDQGWSQAHYEAGLYIQEMLPNTEMVYIEKVNTADRPGTTPAQLAEEL
                     VARGAKVIIFNSDDMKDGAHEFTAAHPEVIVIHASGDGAWEEGNAYQGFANLSNVMPR
                     MEYGKEIAGCAAALSSDSGNIGYLGPLINEETRRLAASAYLGAKYCWTEVAGKDVADL
                     NFEVKWIGFWFNIPGFTSDPTQVADDFFNRGFDVVISGIDTTEALTEAKKFTDEGSTV
                     YAIPYDFKGACDQAPDVCLGVPYYNWGPSYVNYIQSVIDGTYAPTWDYVGPDWADLNN
                     PDTTSLGFVKGAALSEENGTKLDEFIAALAGGLNLWTGPLNLQDGTPFLADGEVATDY
                     QVWYLPQLLEGMVGLSATAAE"
     gene            11777..13243
                     /locus_tag="PLL88_05485"
     CDS             11777..13243
                     /locus_tag="PLL88_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ01052.1"
                     /translation="MEIEIKNICKYFGKIHANDGISLTIHQGEILGILGENGAGKTTL
                     MKILAGLICCDSGEIWIDGKPVRLSSANAALKLGIGMLSQDPLDFPMLKVFDNLYLGS
                     PKGLFIQKKTFRKKIEEEEELLGFHFDQDDLVSNLSVGERQQLEILRLLLFGSELIIL
                     DEPTTGISAQQKKQLFDLLKRLTRLGKSIILVTHKLSEVNELCARTVVLRQGKLVGEV
                     ALPCDSSSLIELMFGKTLEFRNIDDVSVPQQHFRVKDLVIKDARFYSNPVNLDIRKGE
                     ILGLAGMEGSGQRTFLHTLAGIEKPVKGRILDDGDPILKITPYSFSERGIYFMPAARL
                     EEGLVAGMTVEEHFILSQREDDFLINHRRSLATTRARIREFAIKGFSYSAAEELSGGN
                     QQRLLLAMLKKNARLLLLENPTRGLDVESTNWIWTKLHERCQKGTSIIFASADIDELL
                     RFSNRIVIFFGGKISDPYESHELTSEKLGALIGGEGWK"
     gene            13234..14307
                     /locus_tag="PLL88_05490"
     CDS             13234..14307
                     /locus_tag="PLL88_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01053.1"
                     /translation="MEIKNKSHKLLKHFSFSLVAVAGALIFVSIILWIADAQPGLTFQ
                     KLIQGAFGDATKIANGFVVWVPLSLVAGAFIITFTAGLWNLGIEGQIMLGAIFTTGLM
                     RSMQTSALPPAVIIFLSCLMGMVGGALWASLAGILRLYGGVSEIFAGVGLNFVATAFS
                     IWLIFGPWKRPGVASMSGTVPFDEKLWLVTIPGYRLSVWSLILVAVVFVGLFVVLKKT
                     RFGIQIKAIGKNSKAASLMGIQTRKLMMSAMLLCGAIAGLAGALQVVGVYHRLIPSIS
                     SGYGFLGMLVAMLANNDFILTLLVAYLFSILNIGGIQLPIDLHLDSTLSGVLQTALVL
                     FFLIMNGVKQRFLEKAERKQDGE"
     gene            14297..15289
                     /locus_tag="PLL88_05495"
     CDS             14297..15289
                     /locus_tag="PLL88_05495"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01054.1"
                     /translation="MESDFLVGLASVIAFASPIVIAAIGETLTEKSGMLNLSVNGIIL
                     LSAMAGFVAAYQTQNLVAGYLAGALVGAMIGFVLAFCSITLNQSQVAVGLVLAFLCRD
                     LSYFLGNDFMGFSAPVIHSAPIPFLSDLPIVGMLFFDHTVMVYLSLISIPIATVWLNK
                     TRFGLLARSVGERPEAAFARGINVNGVRYLYVILGSAMVGLAGPIYSLGLKPGWKGSM
                     TGLDGIGWIVLAITIFGGWNPFKVSLGSYLFEFLRWIGLVYQTKFTNVPSQVLQVAPF
                     PLMILTLVLVNIKNAEWYEAVIRLLPPPLYKLLTLKGKRKKAAPSSLGIPFRNV"
     gene            15299..16540
                     /gene="thrC"
                     /locus_tag="PLL88_05500"
     CDS             15299..16540
                     /gene="thrC"
                     /locus_tag="PLL88_05500"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOJ01055.1"
                     /translation="MKKFHAYRCSICQTTFEPEDIFYACPHDGGNVDILLDYDSLRKL
                     PLVELVTQSRDFSIWRYLPLLPVSDPGFEKTPLHAVGWTPVYESIALSQQSGLERLWI
                     KDDGRNPTASYKDRASAVVVARAAEVNAEIVVTASTGNAGAALAGMAAACGQKAIIFA
                     PKTAPQAKIAQLLVFGARVILVDGNYDQAFDLSLQASQEFGWYCRNTGYNPFTAEGKK
                     TAAFEIWEQLIVSNKDVSEKLCVFVSVGDGNIISGIHKGFKDLKELGLIDSLPRIFGV
                     QSEGSAAIANAFEEKSEEIKPVHATTLADSISVDLPRDGLRAVRAATQTHGAFIKVSD
                     QEILSAIGKLGKAGVFSEPAGATAYAGVLKASSEGIIQADDPVVCVITGSGLKDVKAA
                     MQAVPEAPVIEPSMAALKKII"
     gene            16540..17511
                     /locus_tag="PLL88_05505"
     CDS             16540..17511
                     /locus_tag="PLL88_05505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ01056.1"
                     /translation="MKEGTAQVAKQKPIYSVVAPVFNEVEGLGEFYKVLKSVMDTVEG
                     AWELLLIDDGSTDGSREVIVDLAAQDSHVVPLIFTRNFGHQIAVTAGMDHAQGDAIII
                     IDSDLQDPPQVIPELIAKWKEGYEVVYAVRREREGETWFKRTSASAFYRLIDRITDVR
                     IPLDTGDFRLLDREVLTVLNTMREKQRFLRGMSSWVGFNQIGVEYDRKSRFAGKSKYP
                     FKKMMKLALNAITGFSTLPLQIAEYSGIVLLTFSMIYALVALVFAMLRKPFLPGQALT
                     VFLLLFLGGLLSLFMGIIGEYISRIYDETKGRPLYILRTNPKIKEKK"
     gene            17508..18974
                     /locus_tag="PLL88_05510"
     CDS             17508..18974
                     /locus_tag="PLL88_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOJ01057.1"
                     /translation="MTRIDLTVYEDRLQNAVKRARERNIIIPTFAQMRDPALVPTKIT
                     EDLSGLGLWDVNPRNLFRVTWKNEPKEKGGRYRGVNFIEFPKELTGVNARIVALIGKW
                     FPTGAHKVGASFGCLVPRLVTGQFDPINQKAVWPSTGNFCRGGAYNSTLLACRSIAIL
                     PEGMSKERFDWLKSVAGEIIATPGSESNVKEIYDKCWELRKSGEDLMIFNQFEEFGNY
                     LWHYEITGHAMQEVLEQILGPQDEFRGVTLTTGSAGTIACGDYLKQVFPGSKVAAGEA
                     VQCPTLLENGFGAHRIEGIGDKHVPWIHNIKSTDLVIAIDDNVVVNLARLFNEPEGRK
                     YLVEKGVSEATVSKLGYLGFSGIANVSMAIKMSKYYELDEHDVVLTVLTDSMDLYQSR
                     LKEMHAEYGEYNRTDAAVTFARDLHGLATDSMQELTYADRRRVHNLKYFTWVEQQGKT
                     YEEIIDQWENRNYWTELQGQIPEIDALITEFNQKVGIL"
     gene            18985..19578
                     /locus_tag="PLL88_05515"
     CDS             18985..19578
                     /locus_tag="PLL88_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01058.1"
                     /translation="MKDVPAQTDLVLVCLLPQPRDLEIARVLGWYRIPLRHAPKVLYT
                     DFLAFYQPASFGKEHQWMIEFVARVRGVELTRRKDLLNEEPFHPKAEDEYYKISIGEL
                     VQLDRPILADKWKRITFFYTTGEQLLHANFISDLVIDQSQRELFRNTVMEFHSKYTNT
                     FPPADDMTALQQEFLTYFLELSAKIKKENEIKEADFD"
     gene            19595..21013
                     /gene="hydA"
                     /locus_tag="PLL88_05520"
     CDS             19595..21013
                     /gene="hydA"
                     /locus_tag="PLL88_05520"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOJ01059.1"
                     /translation="MKTLLRSGMIITAAEQFVGDVLIDGEQIRAIGSALQPDADTALV
                     DVSGKILTPGGIDVHTHFDLPMAGTVSSDDHYTGHKAAACGGTTTVFDFVSQDEPSLQ
                     QCVDAWHTKADEKAAVDFGFHMNVTKFNDQVAREMPGLRALGINSVKVFTAYNGRLRL
                     QDGEIFQVMRVARNNQLLVMVHAENGDVIDLLVNEALKAGHFTPEWHALTRPAEGSIE
                     NVFRTICLAQQAKAPLYIVHMNTAEEVRLLQEARGKGLQVMGETCPQYLYFTIDDLRK
                     PDGAKWVCSPPMRSRVDNEGIQELLGKNALQVVATDHCPFFYDGTHPISYEGEQIAIP
                     GKELGKDDFTKIPNGLPGVGDRMIVLWNYGVRKQIITPQQFVALACTNPAKIFGIYPK
                     KGTLMPGSDADIVVWNQNTPLTYGVAKAQHRTDYNLYEGWEMRDTIHKVYLRGKLIVE
                     DGRWLGSRGQGKYLQCGDPQIV"
     gene            21026..>21259
                     /locus_tag="PLL88_05525"
     CDS             21026..>21259
                     /locus_tag="PLL88_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01060.1"
                     /translation="MLIWFALIIYGIAPVTVLFLKRQKREEGLIWLVLLACGLLGSIL
                     LYLSPITSTRSDSPVLWFSSGSMILSIDGYWNNP"
BASE COUNT         5309 a         4756 c         5635 g         5559 t
ORIGIN      
        1 gcgttcagat tcaccttgac cttccacaac cggtgccttc tgcctttcta acatagctgt
       61 ctttaaagat acaagggtgg gtttctaacc caccattact cctgcttgtg agaaaacagg
      121 aagtgaagtg ttgacctgcc aacaagtcag gtagagtgga tttccaaccc accattgctt
      181 tgcttgtgga aaaacaggag gtgaagtgct gacctgccaa caggtcgggt agggtgggtt
      241 tctaacccac cattacccct gcttgtgaga aaacaggaag tgaagtgctg acctgccaac
      301 aggtcgggta ggatgggttt ctagccctcc ctcgggtgat gttttttcta atgagatacg
      361 cccagtaaat tgctgatcaa aatggatacc agaccagccg tcagggtttt caatcccatc
      421 ttcatcagat tctggttggc tacccttccc aggaacatgc ccagtccaaa taacaccagc
      481 agcgcaaaac cgatggcagt gtaatacacc atacggatgt cgctgaaaaa actggcaaag
      541 aagaacggca ccagcaccac cagggatgca aggaaaggcg atagtccatc caccaccgat
      601 acggcaaccg cggcgaagcg cgaagccttt ccgatcttgc tgccattcaa gtctgtcagg
      661 gtgctgtagc tcaattcgtc catttcccgc ttccgttctg cggattcggt caagaatgac
      721 ccccacaatc cggaaatacc aatggcaacc gaggtggtta aacccgtggt gacaacgatc
      781 atagggtctt ccacgccggc agcgtagtgc cccatgagca cacccatgat ggtcagcacg
      841 ccgtcaaatg cgttcatggc aaaataacgc cgggctattt cgccgatttg cgcgatttcg
      901 ttatattccg atatcctctc agcaagatgc ctgagagggt tacttttctt tttcataatg
      961 acttacgggg gtttttagtc ctgcagggtt gccgcatcgt caatgatcat tttcccggcg
     1021 accacttcat caacggaatg gatggatcca ccctggtcgc gaatcatggc agcaacaaca
     1081 tcataatcaa ggtcatcacc ttcaatggtg atcttggcat tttcgacctt gtggtcaatc
     1141 tcagcgatac tgatattgac agcggaaatg ccatctaaca cgcttatttt ttttgcaagg
     1201 tccacaatac tggggtcgat cggctttaac acatccaata ccaatctacg tatttttcct
     1261 atcatctctg gttcttaaaa aagactcctt tccaatgttt agagatatga acgattcaat
     1321 ttataccata ctttcagggt ctcaatgacc ttttatctaa ttcttatata gctcctccac
     1381 ccaagacttg tttttccaca aggcgcaagc gccgtcactt tcgtccaacc ggttgccgct
     1441 gttgcggatg acctgtaaat gtctggaacg cagcgcctgc agcaggctca tttctctcag
     1501 gtttacatcc gagaatgggg agaaggggca gggctgcaga tcgccctggg ggttgatatg
     1561 gatcagccct ttcccacctg ccagacagcc gcccaattcc acctcaccgc ccggaaaagc
     1621 catgaaaagc gcagagtgct tttcacgcag ttcttccagg atcgccagat ggctcatcac
     1681 ctgctgaggt gtcaacgcca ggtcgtgggt ttgcgggtta ataggcaggt agttgatgaa
     1741 gaaaaacacc ttgcagccct tttccatcat atcggtcagg tatgtttcat cgttcaccag
     1801 atcgaaattc cggctggttt gggtgatgga tgtgccgaag agcatctttt cttgtttcaa
     1861 tttggagaag atctgcatgg cgcgttcata aattccaccg ccacgccgta gatcggtatc
     1921 ttgttgatcg ccttcaatgc tgataaccgg aagaatattg cgctgtttct tgatgttttg
     1981 gatggcggca ctatcgagca gggtgccatt ggtgaacagc aagaagatca gactgggaaa
     2041 ttccttgatc gtatccagca gttctttgcg catgagcggt tcgccgcctg ccagaaggat
     2101 cacggaaaca cccagatccc tggcttgttg aatgacagag cgaaactgtt ggggggtcaa
     2161 ttcctcggcg ctggtctgat gcaagatctt ggcgtagcat cctatacaat ccaaattgca
     2221 atcgttggtg atgctgaaga tgaccatggg cgggacctgc actccctgtt tgagaaatgc
     2281 agcgcgtttc ttttcagcca ggcgataggc aagatatagc tttgccgccg agtatgccag
     2341 ccgcgggttg ttcaaactga aacgtacgat ctgcttcaac cccatttcga aactgatgtt
     2401 gaaataagtg gtgatatctt gtttttcttt ttgcatcgct ctcccatttc tactgcgata
     2461 tatacgcaga aagcatgtca tggttttgta aacggggtcc aatgaccctg ttggtttcct
     2521 cataacgaga caaaaccatg ctattgtact ggatgaatgg aaagtgggag aaaactttgc
     2581 ccccaggtga tcctggaagg tgcgcggcgg ggtatgctgc agacggcagc gcccatggac
     2641 tgcaaaccag tgcgggatga cccacgaccg ttcaatgttc catcgtcagc aaagtcattt
     2701 taaagattcc ttaccccctt tcatcccttt ctgtgaaccc gcgtacgctc ccgcaagagg
     2761 ggctcctgga atgattctgc ttttcttttt aatttacgtg gtttccctgc ccgattccaa
     2821 aatagatgaa tcccagctca cgcaggaatt tgctatccca ttggttgcgc ccatcgatca
     2881 acaccggggt gcgcatgatg ctgtgcagac gtcgaaaatc gagttgtttg aattcattcc
     2941 attccgtaac caggatcaga ccatctgcat tttcggcagt ctcataaggg tttttgcaga
     3001 gctgcaggtc aggaatggct ttagcggtgt tttccatcgc ttgcggatcg tatgcgcgta
     3061 cgtgtgcacc ctcattgcgc agtagatgga tgatctcaat ggcgggcgct tcgcgaatat
     3121 catccgtatt gggtttgaat gatagaccta gaatgccgat atttttatcg accagtgatc
     3181 cacccaccag gttgcgcaat ttcatcacca cttttcggcg ctggttgcgg ttgatatcca
     3241 tcaccgcttg cagcagttgt gggtgagtgc catgcagcac agccatgtgg gccagcgctt
     3301 ttacatcttt tgggaaacaa ctgccacccc accccagccc tgcatccagg aaagcatgac
     3361 cgatgcgttt gtctaacccc attcctcggg cgacttcacg gatatccgct cctagctcat
     3421 cgcagatgtt ggcaatttca ttgatgaacg agatacgcgt ggctaaaaat gcgttggagg
     3481 catacttgat catttctgcg gtgcgcaggt cggtaatcaa agcgggagcg cgcagaggtt
     3541 ggtatagctc tgctactgct tcggctgctt ttcgatgtac cgatcccagc acaacccggt
     3601 cgggttgcat gaagtcgtgg atggcggaac cttcgcgcag gaactcagga ttggacacta
     3661 cgtagtattc cagcgtgttt tgaccgcgtt ttttgcggat gatatctgct acccaatcgc
     3721 ccgtccctac cggcacggtg gatttgttga cgatgatgat cgggtggtcg atcacttcag
     3781 cgatcttttc ggcagcgtcc cgcacatagg tcagatccgc ttcgccatct acccctgaag
     3841 gagtgcccac cgcgatgaaa gcaaattcgg cgtctttcaa tgcttcggta tactcggtgg
     3901 tgaaaaataa acggttggca gccacatttc tttcaaccac ctcttccagc cctggctcgt
     3961 agatcggcat gatgcctttc ttgatattgg caatccgttc tgcattgata tccagacaat
     4021 gaacagtgtt gcccaggtct gcaaaacata ccccagtcac caatccaaca tagccggttc
     4081 cgatcacgca aattgtactc atagcttttt ctccaaagca aagattatag cctaagaaac
     4141 gggctattca tgagtaaacg ccctgattat tgtataatcc ttttactctt ttgtgttggg
     4201 agttgaaaat gacagcagcg atgattgatg gaaaacaaat tgcaaaggat atgcgttccc
     4261 ggttgaccga acaggtggct gagagaaaac gcttgggaaa agctgtaccc ggtctggcta
     4321 cggttttggt tggtgaagac ccggcatctc aggtgtatgt gcgttccaag cataaggcgt
     4381 gtgaacaatt gggcatcaca tctttcagcc atgtgttgcc tgcgtctact ccccaggaag
     4441 agttggtaga actggtcgat tctctcaacg gcgatccaaa agtgcatgga attctggttc
     4501 aacttcccct gcctgccgga ttcgatgaag aagaagtgct gctcaaagtt tcagtaaaaa
     4561 aagatgtgga tggtttccat cccatcaata taggcaaact ggcacaaaaa gggcgtacac
     4621 caagttttat tccgtgtacg ccggcaggtg tgatcgaact gcttcgatca accggcatct
     4681 cgatggaagg aaagagcgcg gttgttttgg ggagatcgaa cattgtgggt atgccggtag
     4741 cccttttgct tgtcaaagaa aatgccacag ttaccatctg tcactcacgc tcccataacc
     4801 tgcctgacct cacccgcggg gcagatatcc tggtggcgtg tgttggtaaa gctgaaatgg
     4861 tgcgtggcga ctgggtgaaa cctggagcgg tggtcatcga tgtgggaatc aaccgtgtgg
     4921 atgacccttc tgctgaaaaa gggcatcggc tggttgggga tgtggctttt gacgaagtga
     4981 gggaggtcgc ttctgcgata acccccgtcc cgggtggtgt cggaccaatg accatcgcca
     5041 tgttgatgca aaacacactg cgtgctgcgg aattggcaga ctgattgaat aaactcatcg
     5101 ttgaaaataa tgaaatgacg ccagaaattg tccgacaaat gatggtatga ttaatgtgtt
     5161 aaaaaaccat actatcaaag ggcactcata tggctacttt gcttgtcaag aatatccaca
     5221 cgctggttac catggatcca gcgcgtcgcg agattcgcaa cggggggatg tttatcaagg
     5281 atggttttat tcagcaggta ggcggttctg acgaggttac agcagatgcg gatgaagttt
     5341 atgatctgaa aggtcatatt gttttcccgg gcttcatcaa tacccaccat catttttacc
     5401 aaacccttac ccgtgtagta cctgctgcac aagatgccaa cctgttcaat tggttgaaaa
     5461 ccctttaccc gatttgggcg cgcatgactt cagaagatat ctatatttcc accaaaaccg
     5521 cgctggcgga gctggcactt tcaggttgca caaccgcgtc cgatcatttg tatttattcc
     5581 ccaatggttc cagcctggat gatgagatcc gggcagctca agaaatgggc atgcgcatcc
     5641 atgcatcgcg tggttccatg agcttgggtg agagcagggg cgggttgccg cccgatagtg
     5701 tggtagatga tgagggtttt atcctcaaag acagtcaacg cttgatcgaa aaataccatg
     5761 atcccaagcc gggttcgcgg gtgcagatcg tgctggcgcc ctgttctccc ttcagcgtga
     5821 ccggtgatct gatgcgcgaa agcgctagcc tggctaggca atatggggtg catttgcata
     5881 cccacctggc tgaaacggag gatgaacagc aattcacggt acagcaattc gggctgcgtc
     5941 ccctggcgta tatgcagtca ttggaatggg taggggaaga cgtgtggttt gcccactctg
     6001 tttatatcga tgaagaagaa caaaaggtgt ttgctgcgac cggttgcggg gtggcgcact
     6061 gcccaacctc taacatgcgc ctggcttcgg ggatcgcccc ccttcgcgaa tatatccggt
     6121 tgggggtaaa agttgggctg ggcgtagatg gttcagccag taatgacggc tcccacatgc
     6181 tggaagaagt gcgccaggca atgctcttgg ctcgcgtgcg tgctggtttg gaaggggcgt
     6241 ctctttccca atccggaact tctcccataa tgacagcgcg gaaagccctg gaactggcaa
     6301 ctctgggcgg cgcccgtgtg ctggggcgcc aggatatcgg gtcgctggaa gtgggaaaat
     6361 gcgcagactt caccgccatt gatctcaacc acatctcgta tgctggcggg ttgcacgatc
     6421 ctgtcgccgc agttgtattt tgtcatccca gaaatgttga ttacaccttc gtgcatggaa
     6481 aagcagttgt tgaaaaagga caactcttat ctatggacct ggtggagcat gtgcaaaaac
     6541 acaacaaggc ggctgccagg ttattaatgg aagagtagat cagaggagtt tttcttgcag
     6601 aatatttcac catatcaacg gctacagacc gaactggaac agatcatcaa cgccaagggg
     6661 gcaggggcaa aactgccttc ggaaccatta ctggcgaaga atttaggagt ttcccgctcg
     6721 accctgcgtg aagccatgcg cacgtttgag gtgcaaggct tgatcaggcg caggcaaggg
     6781 caaggtactt ttgtggttgg aaaacaaaaa acgtttgaag gaggtttaga agtattagag
     6841 agtattgaga cattatcaca acggatcgga ttgaatgtta gcatgggtga attgcaggtc
     6901 aaacggtaca gagtggagaa ggagaatcaa tcgtatgtga atttcccttt gaaagcagca
     6961 gtcatccaaa tcgatcgcac catcattgca gaagaacgtc ctatcgccta tctgatcgac
     7021 atcattccgg ttgctgtact ggatgagaat gatctgcaac aaggttttga cgggtcagtg
     7081 cttgattggc tgctaaaacg tggagatccg gtgttgagcc agtctcacgc acgcatcgat
     7141 tccattgcgg cttccagcga gattgcccat aaattacagg ttcaacgcgc tgctccgctt
     7201 ttattgatgg aatcggacgt atatgatatt tctggtcaat tggtcactca ttcctatagc
     7261 tatttcattc ccggtttttt cgattttcat attgtaagaa aagttgcaaa caaagaattc
     7321 taaacaattg gaggtttacg atgagaagtt tcttaggacg cgacatttta tcgctaaaag
     7381 atttcgaacg agcggagttt ttccgcttat ttgaagtcgc tctcgaaatg gatgagattg
     7441 cccgcaaccg caggaactcg gatattttga aagacaaaac cctggtaacc gccttctacc
     7501 aacccagcac gcgcacccgc ctggcacacg aagctgccat gcaccgcctg ggggggcacg
     7561 ttaccgggtt ctctgatgcc aaaatgaccc gcgctgggga ctggtaccag gagtcgatca
     7621 aagataccgt gcatatgctg gagtattacg gtgatgccat cgtgatgcgc catttccagc
     7681 agggcgctcc agccgaggct gccaaatggg caagcgtgcc catcatcaac gctggcgatg
     7741 ggtggggcga acatccgacc caggttttga ccgatctcta tacggtctgg aatgaactgg
     7801 gcaccattga tggactgacc ttcctgtgca tcggtgatat gcgcatgcgc accatgcatt
     7861 ccatttctta cgctctgacg caatttgaca cggaggtcta ctatgtttgt ccacccgaaa
     7921 tgtcattgac cccggaattc aaaaaagaat tggatgagaa gaacctggtc tattatgagg
     7981 cagatcatgt agccgatgtg atcgataaag cggatgtgat ctatatggaa cctgtcgtgc
     8041 agcccgatta caccaaagcc cgccaggaag cctcagagga taagggtttg acccctgata
     8101 attacaaggt aacccgccaa ttgctgaaag aaaaagccaa acccgattcg atcattctgc
     8161 actcattgcc ccgcatggat gaacttccgc ccgatgtgga tggtacccgc catgcccgct
     8221 attgggaaga agcttttaat ggtgtcgtaa tgcgtatgtc catcctggct tctgttttgg
     8281 gcgcaaccga ataaatgaac ctgtggtggg cgggatgttc ttaccccgcc cattttgtta
     8341 aggagatatt atgcaaacga atttacatgg tagagatttg attggcgatc ttgatttcac
     8401 caaagaagaa gtggaaaccg tgttggatgt tgcctgggat ttgaaacgca agcgcgcatt
     8461 gaacgaaccg catgcctatc tgcgcgataa agtgttggcg atgctgttct tcttcagcag
     8521 cacccgcacc cgtggctctt ttgaagcagg tatggcgcag ttgggcggtc atgcagcgtt
     8581 tattgaaagc aagacaaccc agattgcaca tggtgatacg gaaaaagaga tcggtgatat
     8641 ctttggacgg tattttgacg gtattgccat ccgccatgtg gaatggggca ccggcaaccg
     8701 ctatttgaac ctggtagctc aggcttcgcg cgttcccgtc ctcaacatgc agtgcgagat
     8761 ctatcatcca ttccagtgcc tggcagactt gatgaccatt atggaaaaga aagggcgcga
     8821 cctacggaaa aagaaaatgg tcgtttcatg ggcgtatgcg gaatcgtatt ccaaaccgat
     8881 ctcggtaccg cagagcttga ttctgcaaat gccgcgcttt gggttggatg tcacactggc
     8941 gcacccgccc gagttcgtcc tgatgcctga aatcatggag caagccaaag ctgaagcgaa
     9001 aaaatatggc accaactttg agattgtgca tgatatggat gaaggattca aggatgcaga
     9061 tattgtgtat gccaaatcct ggggaccgct ggtgtatacc acggatgctg aaaaaggtgc
     9121 agagatcacc aaaaagtatt ccggttggat taccaatgag agccgcatgg cgctggctca
     9181 agaagatgcc atttacatgc acccattgcc tgccgaccgg aatatcgaag tgaccgatgg
     9241 cgtgatcgat ggtccgcatt ccgtggtgtt tgatgaagcc gaaaaccgtc tgcacgcaca
     9301 aaaagctgtc atggcattga caatgggata accgatctat gaaaaaacaa gtagcagttg
     9361 tagcgattgg cgggaattcg ctgatcaaag ataacaagaa cgtaacagta gaaagccagt
     9421 atgaggcagc caaagagacc acctatcata ttgctgatat gatggaagcc ggttgggatg
     9481 tggctatcgg gcacggcaat ggtccgcagg tgggttttat tcttcgtcgc tctgaaattg
     9541 cccacaaaac cgctggcatg catgaagtac cgctggatgt gtgtggcgcc gatagccagg
     9601 gtgctattgg atatgccctg cagcaaacgt tgcaaaatga gctctatcga cgcggaatta
     9661 aaaaacccgt ggcgacggtg atcacccagg tgctggttga taaaaacgat aaggcatttg
     9721 tgaatcccac aaaacccatc ggtggtttca tggacgaagc ggaagccaag caaaaggaaa
     9781 aggaactggg ttggaatgtt gtggaagatg ccggaagagg ttggcgccgc gtagttgctt
     9841 cgcccattcc gcaagaagtg gtcgaattgg atgcggtgaa agatctcatc gaagccggag
     9901 tcatcgtgat cactgttggc ggtggaggaa ttccggtcgt tgatgccggt aaccggcaat
     9961 tggttggcac tgctgcggtg atcgataagg attatgcgag cagcttgctg gcgcgtgaga
    10021 tcaaagcgga tttgttcctc atctccacag cagtagaaaa agtatcgctc gactttggaa
    10081 agcccaatca acgccaagtg gatcacatga ccctttctga ggttaagcaa tatctggcag
    10141 aaggcaccca tttcgccaaa ggctccatgg ctcccaaaat tcaggcgatc atctggtacc
    10201 tggaggctgg tggaaagaaa gcgatcgtta ccaatccaga aaatattggt cgtgctttaa
    10261 gaggtgaaac cggaacagtg atagaacccg attaatgttc caaggatgat ttaagcttaa
    10321 ataattgtct atacaattga ataaaaagac ctgttgatgt aaaatgccga attgtgtttc
    10381 gtgttttttt acgaaacaat acttttggag gagaggtgag tatcgaacaa tcaacaggtc
    10441 tttgactatt taaaatgcta tggaggagaa aatgatttct aataaaatga aagcgatttt
    10501 tgcattgctg atagcggcga gtttgctgtt ttcagcgtgt gcaaaggcaa gtgaacctga
    10561 ggaagtagcc gaaccggtcg ctgcggagga aagcgagcca tttgtttttg gcattctcat
    10621 ggttggtgct tacaacgatc agggttggag ccaggctcat tatgaagccg gtttatatat
    10681 tcaggaaatg ctcccgaata ccgagatggt ctatattgag aaggtgaata ccgctgatcg
    10741 cccgggaact accccggctc aattggctga agaacttgtg gcaagaggcg cgaaagtgat
    10801 catcttcaac tcggatgaca tgaaagacgg cgcccatgaa tttaccgcag cccatcctga
    10861 agtcatcgtc attcacgctt ctggtgatgg cgcctgggag gaggggaatg cctaccaggg
    10921 ttttgccaac ttgagcaatg tcatgccacg catggagtat ggcaaagaga tcgccggctg
    10981 tgctgccgct ctttcttctg acagcggcaa tattggctac ctgggtccgc tgattaacga
    11041 agaaacgcgc agattagccg cttcggcata cctgggcgca aaatattgct ggacggaagt
    11101 tgccggtaaa gatgttgccg acctgaactt tgaagtgaaa tggatcggtt tctggttcaa
    11161 catccccggt tttacctctg acccaactca ggtggcagat gatttcttta accgcgggtt
    11221 tgacgtagtg atttcaggca ttgatactac cgaagcattg accgaagcga agaaattcac
    11281 cgatgaaggt agtacggttt atgcgatccc ctatgatttc aagggtgcct gcgatcaagc
    11341 gcccgatgtc tgtctgggtg ttccctacta caactggggt ccaagctatg tgaactatat
    11401 ccaatcggtc atcgacggca cttatgctcc cacttgggat tatgttggtc ctgattgggc
    11461 ggatctcaat aatcccgata cgaccagttt aggatttgtg aaaggtgctg ctctttccga
    11521 agagaacggt acaaagttgg atgaattcat cgctgctttg gctggtggat tgaacttatg
    11581 gaccggtccc ctcaacctgc aagatggtac gccgttcctg gctgatggtg aagtcgcaac
    11641 cgattatcag gtttggtatt tgccgcagct tttggaaggc atggttgggc tgagcgcaac
    11701 tgctgctgaa tgaattgttc aaaataatag acgggaaaaa catcttgttt ttcccgtctt
    11761 ctataattta agaatcatgg aaattgaaat caaaaacatt tgtaaatatt ttggaaagat
    11821 tcatgcgaat gatggcatat cgctgacaat ccaccaggga gaaatattag ggattttggg
    11881 ggaaaacggg gcgggcaaaa cgaccttaat gaagatcctg gctgggttga tctgctgtga
    11941 ttctggagag atctggatcg acgggaaacc ggtgcggctc tcctccgcaa atgccgcgtt
    12001 gaaattaggg atcggaatgc tttctcagga tccgttggat tttcctatgc tgaaggtctt
    12061 tgataatctt taccttggca gcccaaaagg gcttttcata caaaaaaaaa cctttagaaa
    12121 aaaaattgaa gaagaagagg aattgcttgg atttcatttt gatcaggacg atctggtgag
    12181 taacctcagc gtcggtgaac ggcagcaact ggagatttta cgattgttgt tatttggatc
    12241 ggaacttatc attctggatg agccaacaac cggtatcagc gctcaacaaa aaaagcagtt
    12301 gttcgactta ttaaagcggc ttaccaggct tggaaaatct atcattttgg ttacacacaa
    12361 actgtcagaa gtgaatgaac tgtgtgcacg tactgtcgtg ttacgacagg gaaagttagt
    12421 gggtgaagtt gccttgccct gtgattcttc atcgttgata gagctgatgt ttggaaaaac
    12481 gcttgaattt cggaacattg atgatgtttc cgtaccccaa caacattttc gcgttaaaga
    12541 tctggtgata aaagatgctc gcttctattc caatccggtg aacctggata ttcgcaaagg
    12601 cgagatcctt gggttggcgg gcatggaagg gtcggggcag agaacatttc tccatacgct
    12661 ggcgggaatt gaaaagccgg taaaaggcag aattttggat gatggcgatc cgatcttgaa
    12721 aatcactccg tattcttttt cagaaagagg catctatttt atgcctgccg ctcgactgga
    12781 agaaggattg gtagcgggta tgaccgtaga agagcatttt attctttcac aaagggagga
    12841 tgactttttg atcaaccacc gcaggtcgct tgcgacaacc agagcgcgaa tcagagaatt
    12901 tgcgatcaaa gggttttcat attctgctgc cgaagaattg tctggtggaa atcagcagcg
    12961 tttgcttttg gcaatgttaa agaaaaatgc cagattgctg cttcttgaga acccaacccg
    13021 tggattggat gttgaatcca caaactggat ctggacaaag ctgcatgaac gttgtcagaa
    13081 aggaaccagc attatttttg cttctgctga tattgatgaa ttgctccgct tcagcaaccg
    13141 gatcgtgatc ttctttggtg gaaagatttc tgatccttat gaatctcatg aattgacttc
    13201 agagaaatta ggagcgttga tcggaggaga aggatggaaa taaagaacaa gagccataaa
    13261 ctgttgaaac atttttcttt ctctctggtt gccgttgcag gagctttgat ctttgtgtct
    13321 attatccttt ggattgccga cgctcagcca gggctgacgt ttcagaaact gatccagggc
    13381 gcttttggag atgccaccaa aattgctaat ggatttgttg tgtgggttcc gctctcgctg
    13441 gtggctggtg ccttcattat caccttcaca gccggattat ggaatttggg tatcgagggg
    13501 cagatcatgc ttggtgccat tttcactacc ggattgatga gatcgatgca gacttcggcg
    13561 ctgcccccag cggtgattat tttcctgtcc tgcttgatgg gaatggtggg aggggcgcta
    13621 tgggcttctc ttgcgggaat tttacgctta tacggtgggg tgagcgagat attcgcggga
    13681 gtggggctga actttgtcgc gacggcgttt tccatttggt tgatttttgg accatggaaa
    13741 cgccccgggg tggcttcgat gagcggcacc gtaccctttg acgaaaaatt atggttggtc
    13801 acgatccccg gataccggct gagcgtctgg tctctcatat tggttgcggt agtttttgtg
    13861 gggttattcg ttgtattgaa aaagacccgt tttggtatcc agatcaaggc aatcgggaaa
    13921 aattccaaag cagccagttt gatgggcatt cagacaagaa aattgatgat gtcggctatg
    13981 ctcctgtgtg gcgcaattgc cggtttggct ggtgcattgc aagtggtggg tgtatatcat
    14041 cggttgatac catcgatatc ttcaggatat gggtttttgg gaatgttggt cgccatgctg
    14101 gcaaataatg atttcatcct cacccttttg gttgcctatc ttttctcaat cttgaatatc
    14161 ggcggaattc aattaccaat tgatctccac ctggattcca ccctttcagg agtgttgcaa
    14221 actgctcttg tgcttttttt cttgatcatg aatggggtca aacagcgttt cttggaaaaa
    14281 gcggagagga agcaagatgg agagtgattt tctggttggc ttggcaagcg tgatcgcgtt
    14341 cgcttctccc atcgtgattg ccgcgatcgg cgaaacacta acggaaaaat caggaatgct
    14401 aaatctttcc gtgaacggaa tcattctgtt atctgctatg gcaggttttg ttgcagccta
    14461 tcagactcag aacctggttg cagggtatct ggcaggtgcg ctggttggag cgatgatcgg
    14521 ttttgtgctg gctttttgca gcatcacctt gaaccaatcg caggtagctg ttggattggt
    14581 tctggctttt ttgtgtcgcg atctatccta tttcctagga aatgatttca tgggattcag
    14641 cgctcccgtt attcattctg caccgatacc gttcttgagc gacttgccca ttgttggaat
    14701 gttatttttt gatcatacgg tcatggtgta tctcagctta ataagcatcc ctatcgctac
    14761 agtctggctt aataaaacgc gcttcggatt gcttgccagg tcggttgggg aaagaccgga
    14821 ggcggcattt gccagaggta tcaatgtaaa cggcgtgcga tacctttatg taatcttggg
    14881 aagtgcgatg gtcggactgg ctggaccgat ctattctctg gggttgaagc ctggttggaa
    14941 aggatccatg accggtctgg atggcattgg ctggattgtt cttgcgatca ccatttttgg
    15001 ggggtggaat ccttttaagg tatctttagg atcatatctt tttgagttct tgcgctggat
    15061 tgggctggtt taccagacaa aatttaccaa tgtcccttca caggtgttgc aggtggctcc
    15121 ttttccattg atgatcttga cccttgtatt ggtgaacatc aagaatgcgg aatggtatga
    15181 ggctgtcatt cgtctgttgc cgcctccatt atacaaactt ttgacattga aaggaaagcg
    15241 caagaaggct gccccctctt cactaggcat tccatttagg aacgtgtaga atataggcat
    15301 gaaaaaattt catgcctatc gttgctcaat ttgtcagaca acatttgaac cggaagatat
    15361 tttttatgcc tgccctcacg atgggggaaa tgtggatatt cttctggact atgactctct
    15421 aagaaagctg ccgcttgttg agctggtcac ccaatcgcgc gacttttcca tctggcgcta
    15481 tttacccttg ctgcctgttt cagaccccgg gtttgaaaaa acccccctgc atgcagtggg
    15541 ttggacgccg gtttatgaat ccatagcgtt gtcgcaacag agcggattag aacgattgtg
    15601 gatcaaggat gatgggcgga accccactgc gtcttacaaa gaccgtgcca gtgcggtggt
    15661 tgtggctcga gccgctgagg tgaatgcaga gatagtggtc actgcttcaa ccggaaatgc
    15721 cggggcagcc ctggcgggta tggcggctgc atgtggacaa aaagcgatca tctttgcacc
    15781 caaaacagca ccccaagcca agatcgccca gttgctcgtt tttggagcgc gggtcatcct
    15841 ggtggatggg aattatgacc aggcttttga cctgtcgctc caggcgtcgc aagaatttgg
    15901 ttggtattgc cgcaatactg gctataaccc tttcaccgct gaagggaaga aaaccgccgc
    15961 ttttgagatt tgggagcagc tcatagtatc caacaaagat gtatccgaaa agctctgcgt
    16021 ctttgtttcg gtgggagatg gcaacatcat ctctggcatt cacaagggat ttaaagattt
    16081 gaaagaactc ggtctcattg attcgttacc gagaatattt ggcgtccaat ctgaaggatc
    16141 ggcagccata gccaacgcct ttgaagaaaa aagcgaagag atcaagcctg tgcacgccac
    16201 gacgctggca gatagtattt ccgtagatct acctcgtgat ggactgcgcg ctgttcgagc
    16261 ggcaacgcaa acccatggcg cattcatcaa agtgagcgat caggaaatat tgagcgctat
    16321 tggcaagctg gggaaagctg gcgtcttctc cgaacccgcg ggggcgacag cctacgcagg
    16381 cgttttgaaa gcgtccagcg agggaatcat ccaggcggat gacccggtgg tttgtgtgat
    16441 cacgggcagt gggttgaaag atgtgaaagc cgccatgcag gctgttccgg aagctcctgt
    16501 tattgaaccg agtatggctg ctcttaaaaa gatcatttga tgaaagaagg aaccgcgcaa
    16561 gtggcaaaac agaaaccgat ctattccgtg gtcgctccgg ttttcaatga agtggaaggg
    16621 ttgggggagt tctacaaagt gctgaaatcg gtcatggaca cagtggaggg ggcatgggaa
    16681 ttgctgctca ttgatgatgg ctcaacggac ggttcgcgtg aggttattgt ggaccttgct
    16741 gcacaagata gccatgttgt cccgctgatt ttcaccagga atttcggaca ccagatcgca
    16801 gtgaccgctg ggatggatca tgctcaaggc gatgcaatta tcatcatcga ctctgatctg
    16861 caggatcctc cgcaggtgat ccccgaactg atcgcaaagt ggaaagaggg ttatgaggtg
    16921 gtctatgctg ttcgccgtga acgggaaggg gaaacatggt tcaagcgaac cagcgcttct
    16981 gcgttttatc gtttgatcga ccgcatcacg gatgtgcgta ttcctctgga taccggtgat
    17041 ttccgtctgc tcgatcgtga agttcttacc gtgctgaaca ccatgcggga aaagcaacgt
    17101 tttttaaggg gtatgtcgag ctgggtgggc ttcaatcaga tcggcgtgga gtatgaccgg
    17161 aaatcacgtt ttgccggaaa gtccaaatac cccttcaaaa aaatgatgaa actggcattg
    17221 aatgccatca cgggtttttc cactctaccg ctgcaaattg cggagtattc tgggattgta
    17281 ttgctcacct ttagcatgat ttatgcgctg gtcgcgttag tatttgccat gctccgcaaa
    17341 cctttccttc cgggtcaggc attgacggtg tttctattgc tgttcttggg aggattgttg
    17401 tcattgttta tgggcattat cggcgaatac atcagccgca tttatgatga aacgaaagga
    17461 agaccgcttt atattcttcg aacgaatcca aaaatcaagg agaaaaaatg accagaattg
    17521 acctgacagt atatgaagat cgcctgcaaa atgcagtcaa gcgtgccagg gaacgcaata
    17581 ttattatccc tacctttgca cagatgcgtg atcctgctct ggttccaacc aagatcacgg
    17641 aagatctatc gggattgggg ttatgggatg tcaaccctcg caatcttttt cgcgtcacct
    17701 ggaagaatga accaaaagaa aaaggtggca gatatcgtgg tgtgaatttc attgagtttc
    17761 ctaaagaatt gaccggcgtg aacgcgcgta tcgttgcctt gatcggcaaa tggttcccaa
    17821 ccggtgcgca taaggtcgga gcatcttttg gctgcctcgt tccgcgtttg gtaaccgggc
    17881 agtttgaccc cattaatcaa aaggctgtct ggccctccac tggaaatttt tgcagaggcg
    17941 gtgcatataa ttcgaccctg ttggcttgcc gttccatcgc gattctgccg gagggcatga
    18001 gcaaagagcg ttttgattgg ttgaaatcag tggctggcga gatcattgcc acccccggaa
    18061 gtgaatcaaa cgtcaaagag atctacgata aatgctggga attgcgcaaa tcaggggaag
    18121 acctgatgat cttcaatcag tttgaagagt ttggaaatta tttgtggcat tatgaaatta
    18181 ccggtcatgc catgcaagag gtgctcgaac agattttggg gccccaagat gaattccggg
    18241 gtgtcacttt gaccaccggt tctgcgggta ccattgcttg tggcgattat ttaaagcagg
    18301 ttttcccggg gagcaaggtt gcggcaggcg aggcagtgca atgccccacg ttgctggaga
    18361 atggttttgg agcgcatcgc atcgaaggga tcggggataa acatgtgccc tggatccata
    18421 acatcaagag caccgatttg gttatcgcga tcgatgataa tgtggtggtg aacctggcaa
    18481 ggttgttcaa tgagccagaa ggtcgcaaat atctggttga aaaaggtgtc tctgaagcga
    18541 cggtctccaa attgggatat cttggctttt cgggcattgc caatgtttcc atggcaatca
    18601 agatgtcgaa atattatgaa ttagacgaac atgatgtcgt cttgacggtg ttaaccgatt
    18661 ctatggacct ttatcagagt cgtttgaagg aaatgcatgc cgagtatggt gaatataacc
    18721 gtacggatgc tgcggtaact ttcgcgcgtg acctgcatgg tttggcgaca gattctatgc
    18781 aggagttaac ctatgcggat cgccgcaggg tgcataattt gaaatatttc acctgggttg
    18841 agcagcaggg taagacttat gaagagatca tcgatcaatg ggaaaaccgc aactactgga
    18901 ccgaattgca gggtcagatc cctgagatcg atgctcttat aacagaattc aatcagaaag
    18961 tcggtattct ataagcaaca tgaaatgaaa gatgttcctg ctcaaactga tcttgtgctg
    19021 gtttgcttgc tcccacagcc gcgcgacttg gagatagcca gagttcttgg atggtatcgc
    19081 atacctctca ggcatgctcc aaaagtgctg tatactgact tcctggcatt ttatcaaccg
    19141 gcatcttttg ggaaagaaca tcaatggatg atcgagtttg ttgctcgagt gcgtggtgtg
    19201 gaattgacca ggcgcaagga tttgctcaac gaagaaccgt tccatcccaa agcggaagat
    19261 gaatattata agatcagcat tggtgagttg gtgcagcttg atcgaccgat tttggctgac
    19321 aaatggaaaa gaatcacgtt cttttatacg accggcgaac agctattgca tgcaaatttc
    19381 atttctgatc ttgttattga tcagagtcag agagaattat ttcggaatac cgtgatggaa
    19441 tttcatagta aatatacgaa cacgtttcca ccggctgacg atatgactgc tctgcagcag
    19501 gagttcttga catatttcct ggagttgagt gctaagataa aaaaggaaaa tgaaatcaag
    19561 gaagcggatt ttgattgaag cgaaagagtg agcgatgaaa actttattac gttcgggaat
    19621 gattattact gccgcagagc aattcgtcgg cgatgttctt atcgatggcg agcagattcg
    19681 tgccattggg tccgccttac aacctgatgc tgatacagcg cttgtggatg tgtcaggaaa
    19741 aattctgact cccggcggaa tcgatgtgca cacccatttc gacctaccca tggctggcac
    19801 tgtttcatcc gatgatcatt acacaggtca caaggcggca gcttgcgggg ggacaaccac
    19861 ggtctttgat tttgtttctc aagacgaacc atctttgcaa caatgcgtgg atgcctggca
    19921 taccaaggca gacgaaaaag ctgcggttga ttttggcttc catatgaatg ttacaaagtt
    19981 caacgaccag gtagccaggg agatgccggg cttgcgtgcg cttggaatca atagcgtgaa
    20041 agtatttaca gcatacaatg ggcgcctgcg cttgcaggat ggggagattt tccaggttat
    20101 gcgggtggcg agaaacaacc agctcctggt tatggtgcat gcagaaaatg gggatgtgat
    20161 tgatctgttg gtcaacgaag cgttgaaagc ggggcatttc acgccagaat ggcatgctct
    20221 tacccgacca gcggagggtt ccattgagaa tgtcttccgt acaatctgct tggctcagca
    20281 agccaaagct ccgctgtata tcgtgcacat gaatactgcg gaagaggtac ggcttctcca
    20341 agaagcgcgc ggaaaaggtc ttcaagtgat gggggagacc tgtccgcagt atttatattt
    20401 cactatcgac gatctgcgca aaccggatgg ggcgaaatgg gtgtgttctc cgcccatgcg
    20461 aagccgcgtc gataatgagg gaatccagga actattaggg aagaacgcgc tgcaggtagt
    20521 tgcaaccgat cactgcccgt tcttttatga tggcacgcat ccgatctctt atgagggtga
    20581 acaaatcgcc attccgggga aagaattggg aaaagatgat tttacaaaaa ttcccaatgg
    20641 gctacctggt gttggtgatc ggatgatcgt attgtggaac tatggcgtgc gcaagcaaat
    20701 aatcaccccc cagcaatttg tggcgctggc ctgcaccaat ccagcaaaaa tatttggcat
    20761 ttatcctaag aaaggaacgc tcatgccggg ttctgacgcg gatattgtcg tttggaacca
    20821 gaatactccg ctcacctatg gtgtggcgaa agcacagcat cgcactgatt ataaccttta
    20881 tgagggttgg gagatgcgtg atacgatcca caaggtgtat ttacggggaa agctgatcgt
    20941 cgaggatgga cgctggttgg gtagcagagg tcagggaaaa tacctgcaat gcggcgatcc
    21001 gcagatcgta taggagccgg gatctgtgct catttggttc gcgttgatca tttatggaat
    21061 tgcccctgtc acagtgctct ttctaaagcg acagaaaagg gaagaaggac tgatctggct
    21121 ggttctgctg gcatgtggtt tgctgggttc cattttgctc tacttgagtc caattaccag
    21181 tacgcggagc gattctccag tgttgtggtt ttcatcaggc tcgatgatcc tatcgatcga
    21241 tggctactgg aataatccg
//
LOCUS       DAOSDS010000033        21191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1077_length_21191_cov_5.739023, whole genome shotgun sequence.
ACCESSION   DAOSDS010000033 DAOSDS010000000
VERSION     DAOSDS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 21191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21191
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1077_length_21191_cov_5.739023"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PLL88_05530"
     CDS             complement(<1..471)
                     /locus_tag="PLL88_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOJ01061.1"
                     /translation="MERSLYTFPKGFLWGSATAAHQVEGGNTNNNWYIWEQTPGKIIG
                     GARAGLACDWWNGRWKEDLTHAVEDGQNAHRLSIEWSRIEPLPGQWDEAAIQAYWEMI
                     KGIKILGLKPMVTLHHFTDPIWIYEKGGWENDETPAFFVRFVEKIVPVLQDLVDM"
     gene            648..2798
                     /gene="glgP"
                     /locus_tag="PLL88_05535"
     CDS             648..2798
                     /gene="glgP"
                     /locus_tag="PLL88_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOJ01062.1"
                     /translation="MTYKKAFPNHYHFPQRINRLGELAYNLWWAWNPAALRLFSELDR
                     PLWDSLNHNPIAFLHKVDQELLENAASDSLYLDEYDSVMQQFDTYMTTEDTWCNRQSP
                     GSLNGKIAYFSFEFGLHECMPFYAGGLGILSGDHLKEASDMGIPLVGVGFIYHQGYFV
                     QKLTEDGWQETSYYHFNFEEMPIIALTDADGKPLTISVQLPDRKVYARTWLIQVGRVP
                     LYLLDSNVERNSAHDRHMTARLYSNDLEIRISQEILLGCGGVRLLKKLGEKVTVWHMN
                     EGHSAFLTLERVHEMMKTGLSYEAAAAAVKKTNVFTTHTPVPAGNDQFPLWLIDKYFP
                     VLWGKLGLSREQFIELGKQSQTWGDTFSMPVLAIRLSERSNGVSELHGKVAREMWNFL
                     WPEKKVDEVPIGSITNGIHVNSWLARRMRLLFDRYLGVSWYDHMDDEELWKRIDLIPD
                     IEIWDIRRHLKRKLINYAIESARMHWSSGKHQPSQVIAGGVLLDQYSLTIGFGRRFAS
                     YKRANLILSDYERLLKIITREDMPVQLIFAGKAHPADEPGKLLIQQVYRAAKDPRSQG
                     RIVFLEDYDLNIAHLMVQGVDVWMNTPRRPNEASGTSGMKAALNGTLNFSVLDGWWCE
                     GYNEKNGWVIGSDQEYDDPNEQDKQDAESIYRILENEILPLYYQTRTMGDLSEQWVSK
                     IKESIRSCAPKFSTRRMLREYMQQMYLPAMQKEK"
     gene            2813..3349
                     /locus_tag="PLL88_05540"
     CDS             2813..3349
                     /locus_tag="PLL88_05540"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOJ01063.1"
                     /translation="MEVFLDPMAWLSAVGIFLVRVLSIALDTVRMIAVMRGKKAITWV
                     LGVATSLLFVLSIGWVMSDLTNPLKLAAYAVGFATGSVVGMNLEKRMAMGHTFLTVIS
                     SCRGAALAEALRSAGYAVTEIPARGKDGTVAVLELAVERKDLSRVRGMISAIDSEAFI
                     TSRDVESVHRGYWGSQRS"
     gene            3442..4674
                     /locus_tag="PLL88_05545"
     CDS             3442..4674
                     /locus_tag="PLL88_05545"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HOJ01064.1"
                     /translation="MDSTVLSIEEARKVIILSQILNTIPKNSGEKNSVTEVVEKLGYV
                     QIDTINVLNRAHHHTLWTRLPHYQSQDLHQAQAEEKRIFEYWGHAMSYLPMADYRYSL
                     PYKQHFSSPYGNWMKERLAKYGQVMESCLKRIQQEGALGSKDFESEESQPGGTWWDWH
                     PVKMALELLFWQGKLMVTERKNFQKVFDLPERVLPAWVDTSMPTQEELGRFLVQRALR
                     SYGLATEREIVDHLRVAERAVIVQALKEMRAANEVQAVLVKKDEAELPYYALPGELEE
                     KLATPETEHDVFIFSPFDNFCIQRARMKTFFHYDYALECYLKPEQRKFGYFALPLFWK
                     HAFVGKMDAKADRKEKTLLIHHLQFEDDFQADDGFQAAFQKCLMAFTEFQNCQRCVIK
                     RVTPATGTHLTGQAFTIE"
     gene            4720..6015
                     /locus_tag="PLL88_05550"
     CDS             4720..6015
                     /locus_tag="PLL88_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOJ01065.1"
                     /translation="MFKSKKTILILIACLILVAGLGLAFNTRVQEWVGWHASQFFSWL
                     KVQLNPPEQVEFHAAGDSTPDLPLLAVTTPTASSSQNDAQPQESAAPTTASVALPDFM
                     ELEGITYFSQHNRWNYCGPANLAMALSYWGWEGTHDDVARVVKPYQKDKSVMPYELVD
                     YVRSETNLDALVRVGGDLRLLKTLIASGYPVIVEKGPYFRDISYRITWMGHYQLLNGY
                     NDATGAFIAQDSYQHANFEQPYADLVDEWRSFNYTYVVVFPANERNDVLNLLGENSDV
                     DANYRLAAQKAVDEIYKLEGLSQFFAWYNYGTNLVYLRDYKGAADAYDQAFALYNALA
                     EDNTMRPYRILWYQTGPYYAYYFMARYTDVINLATKNSLEMVRDDEPALEESYYWRGR
                     AKVAVGDQGGAIDDFRTCLTYHPDFTPCVDELSALGVYP"
     gene            6235..7515
                     /locus_tag="PLL88_05555"
     CDS             6235..7515
                     /locus_tag="PLL88_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HOJ01066.1"
                     /translation="MVRVLHFSDAHIDMVRQGRRDAQSGLPLRTLDFLHALDTIIETA
                     IDQKVALVIFAGDAYRNQMPAPTFQREWGKRILRLSQAGIFTILVVGNHDISPAIGRA
                     HALQEFETLRIPNTHVVSEPCLLKPENLNGLPVQILGLPWISRSGLMAYMETQGKSLD
                     DVHTEIEDALADVLKNLLGQLDASLPAILTAHITVQGALYGNERSVMLGHDLVIPPGM
                     LKDERLDYVALGHIHKFQDLNKGHYPPMVYSGSIEKMDFGEISDTKGFIVADVERGKT
                     TYTFHPLQGRKFLSTEVEVSEQDAITETILKKMPAQELLKDAIYRLLLSYPSKWEALI
                     DEHAIRAYADGAFEFYLVRNPYSGIRVRLSKDMQLGQMAPTELLDLYWRSINMDEKTG
                     KELQRLAAAIIHSSERNESIETLLEALDKESERV"
     gene            7512..10079
                     /locus_tag="PLL88_05560"
     CDS             7512..10079
                     /locus_tag="PLL88_05560"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOJ01067.1"
                     /translation="MIPVRLQVHGFLSYKEQIELSFENFDLACISGDNGAGKSSLLDA
                     ITWVLFGKARRNDDGVINNTCTEAEVIFQFLYENELYRVQRLKARNRSVLLEFQIKDD
                     AGTWKALTECSVAETQEWINRTLHLDYDTFINASFFLQGKADEFTQKNSTQRKEILAG
                     ILGMDVWEHYKDITREARRTLESRMKMIEGSLHEIEEELVQEEQRRQALEQAKAELQE
                     AERQRREQQKLYDQAKSLAELIAEQQKHIDELKERRNVVQTSVQRFEQHMKERLDDEQ
                     NLVQVVDAAKEIEKTFQERVVLKEKLQELDALSSRFYTLQQKQAEAAQSIAVEESRLK
                     QELFSLENERSKIQQMIENSLELEKEKTRLSNELLQLQTEVEQLGAIKTQLDDCVVKK
                     SALQAENATLRAHMQKLKERMERLKAETGSTCPLCGQPLSEEHRRQVVDEIHAEGKQD
                     AENFRKNEELLSKLGDEIITCEKQIVALNDQTQRLRTKEREFGALESELKRLALEIAS
                     WKSDGAPKIKALEERLKERKFAQGVRDQETAISLEMEKLGYDAALHESYRKREQASRD
                     AEQKHQAALIAASRLEALRGEIAILRSQYQAGLDEIEMLNSKLETLENQHQQAASQAP
                     DLDEVEGHLKELIAMENQQRTVVGGAMQLLAVIETQKERKQKIHDDLADLSREQTNFL
                     LLEKAFGRDGVQTLIMEEALPQMQAQANDILRKLSSGSMSVRFETEREYKDKKRDDKM
                     QVLDILISDAEGVQRPYDLFSGGEAFRINFAIRLALSKVLAQRAGARLQMLVIDEGFG
                     SQDAEGRQKLIEAINLIRPDFKKILVITHLEELKDAFPARIEVSKTRKGSQVEVMVQ"
     gene            10076..11194
                     /locus_tag="PLL88_05565"
     CDS             10076..11194
                     /locus_tag="PLL88_05565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ01068.1"
                     /translation="MKDQPLHIGVQQRVLPVYRIPFFDKLAEQYRGKVSIFYGLPRSH
                     EMLEANAVPQLALHTRGRNLHLFSGQFYLCWQFGLLQWLQAWKPNVLIMEANPRYPNS
                     QCAMGWVKRYHRKVIGWGLGSPVAGGLLGGIRMRQRRRFVSQFDALITYSQQGAREYA
                     QLGFPEEYIFVAPNAATARPTIVPPERPLQYALNRPQVLFVGRLQERKRVNTLIKACA
                     KLPTELQPQLHIVGDGPSRGMLEQLAQQYFPETKFYGAMHGKELATLFQKADLFVLPG
                     TGGLAVQEAMSYALPVMVGEADGTQSDLVRPENGWMLERGDVEELAKKMTEAFQDIAA
                     LRKKGSASYAIVRNEVNLENMVAVFEKAIDAVLKGSWR"
     gene            11191..12399
                     /locus_tag="PLL88_05570"
     CDS             11191..12399
                     /locus_tag="PLL88_05570"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ01069.1"
                     /translation="MKIVFVADGRSNITQSWLAEVAGKDIDIFLISTFPCEKPKEAKE
                     MRILPVAFSFLAGGQVKLTGQEQKNSRFRKAFSAFRTWLQKIRYRVGPLTLHFYKRNY
                     LDFLDSIQPDEVHALRIPFEGMLASFTPEKYPLVVSTWGNDLTLHAGGSRRMAKWTRK
                     VLQRANALMADASRDLHLATDWGLRAGVPTLMVPGNGGLHLEYFKQFNQQMECDSFNP
                     QKYQIINPRGFRPGSVHQEIFFNAIPGILKEIPEVQFVCPAMGGQVEAEKRVRELGIE
                     KKVLLLPYLPQAELWHLYYASDIYLSLSSHDGTPNTFLEAIACGCFPIVGDILSMREW
                     IEDGKNGMLVDPTNVEQVVSAVVRALQDEGLRERAQALNWELVSEKADRMKIRSKIRA
                     FYSHLLTVQP"
     gene            complement(12401..13000)
                     /locus_tag="PLL88_05575"
     CDS             complement(12401..13000)
                     /locus_tag="PLL88_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ01070.1"
                     /translation="MSIKAVLWDFSGVLFIPIEGRGHNYYAQSLGLSSETLTKYYKDN
                     LNRKLNLGEIDHKTFYRTMFKEQGINEELLSLYEEVFYQSFQLNIPLVDRIKSIRKTK
                     KVGLLSNYSNRLRPMLENNLKIAGLFDELIISCEVRMVKPDEAIYRLALARLDVQPQE
                     TIFVDDLIENIEAAQKIGIHAIHFQNTAQVISEVGQMLG"
     gene            complement(12997..13635)
                     /locus_tag="PLL88_05580"
     CDS             complement(12997..13635)
                     /locus_tag="PLL88_05580"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ01071.1"
                     /translation="MVAKTIYLVRHGTTEWIEQGHVHGALDSPLSKFGEWEAQQAAGA
                     LLGHNITHIFSSPQGRAMQTAGFIAEKLPAASITPLEGLREMGFGFMEGKPDLYKKYS
                     RFPFLFIFIAPVWHAMLGFTGEKRADLHSRVLNAWQRILAHQDQGNIVVVSHALAINV
                     ILTTLPCGEGMKKRERYHLGTCSISEVRIDEEGKATLAVINNTTHLQEKEAV"
     gene            13912..15489
                     /locus_tag="PLL88_05585"
     CDS             13912..15489
                     /locus_tag="PLL88_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOJ01072.1"
                     /translation="MAFKLTYATMFNPPEEMHTRFEDALKAVRANLGKEHAMIINGKD
                     YFTAEKFEDINPADTSEVLALFQKGNEKDAAAAIEAAQKAFPLWSKTPWQERVKFMRR
                     VAELLDERIYEIGAIVALEVGKNRMEALGDVAETADLIRYACDQMEANDGFIKKLGTD
                     PLSGYTASNVSVLRPYGVWLVISPFNFPAALTGGPCGAALVSGNTLVFKPATDTAWTS
                     RKLAECMRDAGLPDGVFNFVSGPGHSMGQALIENPAVAGITFTGSYDVGMGIYRDFAK
                     GEYVRPTILELGGKNPAIVSRNADIEEAAAGIVRSAFGLQGQKCSACSRVYVEKPVYQ
                     KLLERMIEITKKMTIGDPTDRNTFLGPVVNSRSYQDYQDFLDKIYHSGGRFLTGGKVL
                     KDGEYAKGYFCTPTIVADLPVRNSIWKTEMFVPITTVAYVDSLEEAMQLANDCTYGLT
                     AGFYGSAEETSWFFDTIQAGVTYANRPQGATTGAWPGFQPFGGWKGSGSSGKNAGGLY
                     YVQLYMHEQIQTLIKKA"
     gene            15565..16467
                     /locus_tag="PLL88_05590"
     CDS             15565..16467
                     /locus_tag="PLL88_05590"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOJ01073.1"
                     /translation="MNKLCSLKEAVAEHVKDGDLVYAAGFTHLIPFAVGHEIIRQGRR
                     DLTLARATPDLIYDQMIAAGCARKVIFSYLGNPGVGSLRMVRAAIESGTLEWEEYSHF
                     SMITRLTAGASGLPFMPMNLTAAPDLEKVNPNFKRVRDPFTGNEVMVVPPLHPDVAIV
                     HVQRADAEGNAHIWGILGEQKEAAFAARKVILTAEEIVDEAVIRSDPNRTVIPNLIVD
                     AVCHVPYCAHPSYTQGYYDRDNDFYLQWDEISKSRENVDRWLQEFVFDVQDHSRYWQK
                     LGEATHARLRVKDQFSTPINYGAF"
     gene            16487..17254
                     /locus_tag="PLL88_05595"
     CDS             16487..17254
                     /locus_tag="PLL88_05595"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOJ01074.1"
                     /translation="MSERKYSKNELMIVNAARLLQDSDLVFVGVGTPNLACNLARRTH
                     APNLTMVYEAGVIGAQPARLPLSIGDPTLVSGASGVCSMYEIFSLYLQRGNVDVGFLG
                     GAQIDRFCNINATVIGDYYHPKVRLPGSGGSMEIAAWANRCYIMTPHDKRRFPEKVDF
                     CTSAGFLAGRAEREKSGVRGKGPQAVVTDLAIMMPDEKGELQVTALHPGVELEEVRAN
                     TGWDIQVAAAVERTAEPSVEELRILHEELDPSGIYLH"
     gene            complement(17341..18402)
                     /locus_tag="PLL88_05600"
     CDS             complement(17341..18402)
                     /locus_tag="PLL88_05600"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOJ01075.1"
                     /translation="MNKLAKLVDVIRHWFTEGKDAAIATLVRSSGTTPLPVGEMMAIS
                     SASEMQGAVSRGCVEGAILEKAQDVIRNNHATLQHFGYSDQSAWEVGLTCGGEIDVLI
                     EPFLRSQGSVDFFERIRAQVENEEPFFLLHFLDEKNSGEKIILGREKLLYTDLSAQFM
                     PSPTAIQELWNQPTSHIAEVALVDGKKQPAYIHYFQPASRIVIVGASEIAIYLTKMAK
                     LVGFSVIIVDPRSMFATQARFPDADIILPKWPQDCLPSLNLKKQDALVVISHDEKIDL
                     PALQCGLEKSIAYIGLLGSLKTRQNRFNALKEADWQAQDLARLHAPIGLDIGSKKAEE
                     IALSILCEIVKERNTGKLA"
     gene            complement(18440..19078)
                     /locus_tag="PLL88_05605"
     CDS             complement(18440..19078)
                     /locus_tag="PLL88_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ01076.1"
                     /translation="MKKIAGKINGWDKAYKKEPQQFVEPEKICSLADILFKKYGVKRI
                     LDLGCGNGRHLVYFGKAGYEVYGSDLSGWGLQAASTWLRKESIPSRLSLADMQVLPFK
                     DESFDAIISFRVIQHNLFADIQATFKELERILRKDGLFIGDVLKYDPESDRFKEWEMV
                     EPNTFVPRSGTEKGMPHHAFTKKEVFDLLSAWKIQFFNTPPGKHHFDIIAQK"
     gene            complement(19097..19537)
                     /locus_tag="PLL88_05610"
     CDS             complement(19097..19537)
                     /locus_tag="PLL88_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HOJ01077.1"
                     /translation="MTWFNPATLASLDHIDRAAWEALCDGCGICCLYKVRAPYDPKTS
                     LTCIACRYLDLQTCGCRVYEHRHDLMPTCLDLIPANVRQLTWLPETCAYRRIAEGKPL
                     PWWHHLRSRKRDLVHRLGISVHGIAISEKDMDLNDLQKYTMQPW"
     gene            complement(19537..19764)
                     /locus_tag="PLL88_05615"
     CDS             complement(19537..19764)
                     /locus_tag="PLL88_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01078.1"
                     /translation="MSITLLDGTLQVHIFYEKGDHEFDDNVCICFKEDCPEDEKIFYA
                     GETNIYVTSQQARELAQMLNTAADQSNHASR"
     gene            19908..20831
                     /locus_tag="PLL88_05620"
     CDS             19908..20831
                     /locus_tag="PLL88_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOJ01079.1"
                     /translation="MLLGMKSLGIAALVLMGLLAGCSMPTAQQSTATPLPAPTADLQG
                     KLVFAAKTSAKEDYELFLLDLQSGDIQQLTDNQFEDIAPDFSPDGTQIAYSANPNGSY
                     DLFLLDLDTLTIEALLEDATDKTEPVWSPDGKEIAYQSNGYGDNDIYIITLSDKVVQR
                     LTEEDAQDTQASWQADGKGLAFVSDRDGDFEVFAMDIISRQVSQLTNNEYYDASPVCS
                     PDGEKIAFLSKREGSFNLYVMNADGTEETTLVQDRETNHSPAWSPDGSKLAFISDSED
                     HSGIYMVKIESGEVYLVLDGYFHYAGLSWRQ"
     gene            complement(20884..>21191)
                     /locus_tag="PLL88_05625"
     CDS             complement(20884..>21191)
                     /locus_tag="PLL88_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOJ01080.1"
                     /translation="QLILHSDQGWQYQMNSYQKLLKDKGIRQSMSRKGNCYDNSVMEN
                     FFGILKSEFYHNQNFSSFQHFEKALSEYIDYYNKRRIKAKLKGLSPVQFRTQSFLST"
BASE COUNT         5322 a         5145 c         5756 g         4968 t
ORIGIN      
        1 catatctacc aggtcttgca gaaccggtac gatcttttcc acaaaacgga caaagaaggc
       61 aggggtctca tcgttctccc agccgccttt ttcataaatc cagatggggt cggtgaaatg
      121 gtgcagggtg accatgggtt tgagcccaag gattttgatc cctttgatca tttcccaata
      181 agcctgaatg gctgcttcat cccattgacc cggcagcggt tcgatgcggc tccattctat
      241 ggacaaacgg tgggcattct gcccatcttc cacagcgtgc gtaaggtcct ctttccagcg
      301 cccattccac caatcgcaag ccagcccggc tctggcaccc ccgatgatct tgccgggggt
      361 ctgctcccag atgtaccagt tgttgttggt gttgccgccc tccacctggt gagcggcggt
      421 ggctgagccc cagaggaacc ccttggggaa ggtatataat gatcgctcca ttttcttttc
      481 tcccaatatt ttttatcgca atgcagtgat aattataggt gatagaaaaa tttctgctat
      541 ctgaaaccat atcggaaatc gcttcactta agatagaatt gaggcgcagg tatgttataa
      601 tctcgccttg cttttcaggg caggaataca aaaaaaggaa aaaatcgatg acatataaga
      661 aggccttccc aaaccattat cattttcccc aacgcatcaa ccgcctgggg gaactggcat
      721 ataacctgtg gtgggcttgg aacccggctg cattgcgttt attctcggaa ctggacagac
      781 cgttatggga ttcgttgaat cacaacccga tcgctttttt gcataaggtc gatcaggaac
      841 tgttggaaaa tgctgccagc gacagcctgt atctggatga atacgactcc gtgatgcaac
      901 aattcgacac gtatatgacc acggaagaca cctggtgcaa ccggcagagc cccggttcat
      961 tgaatgggaa gatcgcctat ttctcttttg aattcggatt gcacgaatgc atgccgttct
     1021 acgcaggcgg tctgggcatc ttatcgggcg accatctgaa agaagccagc gatatgggca
     1081 tcccactggt tggggtagga ttcatctacc accagggtta ttttgtccaa aaactgactg
     1141 aggatgggtg gcaggaaacc tcgtattacc atttcaattt tgaagaaatg cccatcattg
     1201 ccttaaccga cgcggatgga aaaccgctta ctatttccgt gcagcttccg gaccgcaaag
     1261 tatatgcgcg cacctggttg atccaggtgg gcagggttcc tctttatttg ctggattcca
     1321 acgtggaaag aaactctgcg catgaccgac atatgacagc cagattgtac agcaatgatc
     1381 tggagatccg catttctcag gagattctgc tgggctgcgg aggggtacgg ctgttaaaga
     1441 aactgggtga aaaagttacc gtctggcata tgaatgaagg gcattcggcg tttttgacct
     1501 tggaacgtgt gcatgagatg atgaaaaccg gtttatcgta cgaggcagca gcagcggcag
     1561 taaagaagac caacgtcttc accacccaca cccctgttcc agccgggaac gatcagttcc
     1621 cgctatggct cattgacaaa tacttccccg tgctgtgggg caaactgggc ttgtcgcgcg
     1681 aacagttcat tgagctcggc aaacaaagcc agacgtgggg agataccttc agcatgcccg
     1741 ttctggcaat ccgcctgtcg gagcgcagca acggcgtgtc ggaactgcat ggaaaggtgg
     1801 cgcgcgaaat gtggaatttc ctgtggccgg aaaagaaagt ggacgaggtg cccatcggtt
     1861 cgatcaccaa tggcatccat gtgaattcat ggctggcgcg ccgtatgcgc ttgcttttcg
     1921 atcgctatct gggagtgagc tggtatgatc atatggatga tgaagaactg tggaaacgga
     1981 tcgacctgat cccggatatc gagatctggg acattcgccg gcacctcaag cgcaaactga
     2041 tcaattacgc catagaatct gcgcggatgc actggtcatc gggaaaacat caaccttcgc
     2101 aggtcattgc ggggggagtg ctgttggacc aatattccct gaccatcggt ttcggtcggc
     2161 gcttcgccag ctataaacgc gccaacctga tcttatcaga ttacgaacga ttgttgaaaa
     2221 tcatcacccg tgaggatatg cctgtgcaat tgatcttcgc aggaaaagcc cacccggcgg
     2281 atgaaccggg caagttgctc atccagcagg tttaccgcgc ggcgaaagat ccgcgctcgc
     2341 aggggcgcat cgtgtttttg gaagattatg acctgaatat cgctcacctc atggtgcagg
     2401 gtgtggatgt gtggatgaac accccccgcc gacccaacga agcgtcgggc acctcaggca
     2461 tgaaagctgc tttgaatgga actctgaact tctcagtgtt ggacggctgg tggtgcgaag
     2521 gatataacga gaagaacggc tgggtcatcg gcagcgacca agagtatgac gatccaaacg
     2581 aacaggacaa acaggacgct gaaagcattt accggatcct ggaaaacgaa atcctgccgc
     2641 tgtactatca aacgcgcacc atgggcgatc tatcggagca atgggtgtcg aagatcaaag
     2701 agagcattcg ttcttgtgct cctaaattca gcacacggcg catgctgcgg gaatacatgc
     2761 agcaaatgta cctgcccgct atgcagaaag agaaataaat caaggagttg tgatggaagt
     2821 gtttttagac ccaatggcat ggctatcagc cgtggggata tttttggtgc gtgttttgag
     2881 cattgcactg gacaccgtgc ggatgatagc ggtaatgcgc gggaagaaag caattacctg
     2941 ggtgctgggg gtggcgacat ccttgctgtt cgtactttcc attgggtggg tgatgtctga
     3001 tctcaccaat ccgctcaaac ttgcggcata tgcagtcggt tttgccaccg gttcggtagt
     3061 gggaatgaac cttgaaaaaa ggatggctat ggggcatacc tttttgactg tgatcagctc
     3121 ttgccggggg gctgcgttgg cagaagcgct gcgcagcgca ggttatgcag taaccgagat
     3181 cccggcgcga ggcaaggacg gcacggtagc ggtgctcgaa ctggcggtgg aacgcaagga
     3241 cctgagcaga gtgcggggta tgatcagcgc gattgattcg gaggcgttta ttacctcccg
     3301 cgatgtcgaa tccgttcacc gcggatactg gggcagccag agaagctgat catggagagg
     3361 ttgttgtaca cccaaccatc ttctttaatt gtcctctata atcaatccag aacggcatga
     3421 ttcctgctca aggagatagc ggtggatagc acagttctca gtatcgaaga agcccggaaa
     3481 gtcatcattc tctcccaaat cctcaacaca atcccgaaaa actcaggcga aaagaacagc
     3541 gtgacggagg tggtggaaaa gcttgggtat gtgcaaattg atacgatcaa cgtgctcaac
     3601 cgtgcgcacc accacacctt gtggacgcgt ctgccgcact atcaaagtca ggacctgcac
     3661 caggcgcagg cagaagagaa gcgcatcttc gaatactggg gacatgccat gagctatctg
     3721 cccatggcag attatcgcta cagtctgccc tataaacagc acttcagcag cccgtatgga
     3781 aactggatga aagagcggct tgcaaaatat gggcaagtta tggagtcttg tctgaaacgc
     3841 atccagcagg aaggggcgct gggctcgaaa gattttgagt cggaagaatc tcaacccggg
     3901 ggaacctggt gggattggca cccggtaaaa atggcgctgg aattgctttt ctggcaggga
     3961 aaactgatgg tgacggaacg gaagaacttt caaaaggtct tcgacctccc agaacgggtg
     4021 ctgcctgcct gggtagacac cagcatgcca acgcaggagg agctgggaag gttcctggtg
     4081 cagcgggcat tgcgatctta tggtctggca acggagcggg aaatcgtgga ccatctgcgc
     4141 gtcgcagaaa gagcggtgat tgtgcaggcg ctgaaggaaa tgcgcgctgc gaacgaagtg
     4201 caggctgtgc tggtgaagaa agatgaggcg gaattgcctt attatgcgct ccccggcgag
     4261 ctggaggaga agctggcaac accagaaacg gagcatgacg tgttcatctt ttcccccttc
     4321 gacaacttct gcatccagcg agcgcgcatg aagacattct ttcattacga ttacgccctg
     4381 gaatgttacc tgaagccgga acaacgaaaa ttcggatatt tcgcgttgcc cttgttttgg
     4441 aagcatgcct tcgttggaaa gatggacgca aaagccgatc gcaaagagaa gacgctgctc
     4501 atccaccatt tgcagttcga agatgatttt caagccgatg acggttttca agccgctttt
     4561 caaaaatgtt tgatggcttt tacagagttc cagaactgtc aacgttgtgt gatcaaaagg
     4621 gtcacccctg caacgggcac tcacctgacg gggcaagcgt ttactatcga ataagtctcc
     4681 atttccgacc aaaattggta tataggttat aatgccggca tgtttaaaag caaaaaaacc
     4741 attttgattc tcatcgcctg cctgattctc gttgccggac tcgggctggc gttcaatacg
     4801 cgcgtgcagg aatgggttgg ctggcatgct tctcagttct ttagttggtt gaaggtccaa
     4861 ttgaaccccc ctgagcaggt agaatttcac gcggctggcg acagcacgcc agatctgcca
     4921 ttgctggcgg tcaccacacc cactgcttcg tcgtctcaaa acgatgcgca gccgcaggaa
     4981 agtgctgctc ccaccactgc cagcgttgct ctccctgatt ttatggagct ggaaggcatc
     5041 acgtattttt ctcagcacaa ccgctggaat tactgcggac cggcaaatct ggcgatggcg
     5101 ctttcgtatt ggggctggga aggcacgcac gatgatgtgg cgcgcgttgt gaagccctat
     5161 caaaaagata aatcggtcat gccctatgaa ttggttgact atgtgcgcag cgaaaccaat
     5221 ctggacgcgc tcgtgcgcgt gggaggcgac ctgcgactgc taaagacgct gatcgcaagc
     5281 gggtatccgg tgatcgtcga aaaaggtcct tattttcgcg acatcagcta ccgcatcacc
     5341 tggatggggc attaccaatt gctgaacgga tacaacgacg caaccggcgc attcatcgca
     5401 caagattctt accaacacgc gaactttgag caaccttatg ccgatttggt ggatgagtgg
     5461 cgctctttca actacaccta cgtggtcgtt tttccggcga atgaacgcaa tgacgtactg
     5521 aatctgttgg gtgagaacag cgatgtagac gcgaactatc gcctggcggc tcaaaaggcg
     5581 gtggatgaga tctacaagct ggaggggctc agccagttct ttgcctggta caactacggg
     5641 acgaacctgg tctacctacg cgattacaaa ggcgcggctg atgcgtatga ccaggcgttt
     5701 gccctgtata atgccctggc tgaagataac accatgcgcc cgtatcgcat tttgtggtat
     5761 cagaccggac cgtattacgc atattacttc atggcgcgtt acaccgacgt gatcaatctg
     5821 gcgaccaaga attcccttga gatggtgcgc gatgacgaac cagccttgga agaaagctac
     5881 tactggcgag gacgagccaa agtggcggtc ggcgatcaag gcggagcgat cgatgacttc
     5941 agaacctgcc tgacctacca ccctgatttc actccctgcg tggatgaatt gagcgcgcta
     6001 ggcgtttatc cctaaaagcc gggcaatcag cgaagactga caacgttttt cgatctttgg
     6061 tttgaaggtg aacagcagcg tgtcgctcgc tcacgctcat cctgcacgcg gcgagggctg
     6121 actgggatgt ggagtgaacc tgacaggaaa gaaaccgaaa cgctcctgat gaacaatttg
     6181 cgcgaataaa gctgtgtgca ttcccgttac ccaggtacaa ttaggaaaga gaacatggtg
     6241 agggtactgc acttttcgga tgcgcacatc gacatggtgc gccagggtcg acgagatgcg
     6301 caaagcggat tgcctttgcg cacgctcgat tttctgcacg cgctggacac catcatcgaa
     6361 acagccattg accaaaaagt agcccttgtc atctttgcgg gtgatgcata ccgcaaccag
     6421 atgccggctc ccacctttca acgcgaatgg ggaaaacgca ttttgcggct ctcacaggct
     6481 ggaatcttta ccattctggt ggtcggcaac catgacattt ctccagcgat tggacgcgcc
     6541 catgccttgc aggagtttga aaccctgcgc attcccaaca cgcatgtcgt cagcgaaccc
     6601 tgtttgttga aacccgaaaa tctgaatggc ttgcccgtgc agatcctcgg gttaccctgg
     6661 atctcgcgct ccgggttgat ggcgtatatg gaaacgcagg gaaagtcgct ggatgatgtg
     6721 cataccgaga tcgaggatgc gctagcggat gttttgaaaa atcttctggg tcaattggat
     6781 gcttctctgc ccgccatcct caccgcgcat atcaccgtgc agggggcgct gtatggcaat
     6841 gaacgcagcg tcatgctggg gcacgatctg gtcatcccac ctggtatgtt gaaagacgaa
     6901 cggctggatt atgtcgccct ggggcacatt cacaaattcc aggatctgaa caagggtcac
     6961 tacccgccga tggtttacag cggctccatt gaaaagatgg atttcgggga gatcagcgat
     7021 acgaagggtt ttatcgtagc ggatgtggag aggggcaaaa ccacctatac tttccatccc
     7081 ttgcagggca gaaaatttct ttctacggag gtcgaggtca gcgagcagga tgccatcact
     7141 gaaacgattt tgaagaagat gcctgcccaa gaattattga aagacgccat ttaccggttg
     7201 ctcctgagct atcccagcaa atgggaagcg ttgatcgatg aacatgccat ccgcgcatat
     7261 gcggacggcg cttttgaatt ctatctggta cgcaacccgt acagcggcat tcgtgttcgg
     7321 ctttcaaaag atatgcagct tgggcagatg gctcctaccg aactgctgga cctttactgg
     7381 aggtccatta atatggatga aaaaaccgga aaagaattgc agcgtctggc ggctgccatc
     7441 atccacagca gcgaacgcaa cgaatcgatc gaaaccttat tggaagcgct ggataaggag
     7501 tctgaaaggg tatgatcccg gtcaggttgc aggtgcacgg attcctctcg tataaagaac
     7561 agatcgagct gtcttttgag aattttgatc tggcttgtat ttccggcgat aacggcgcag
     7621 gaaaatcttc tctgctggat gccatcacct gggtgttgtt cggaaaagcg cgccgcaatg
     7681 atgatggtgt gatcaacaat acctgcacgg aagcagaagt gatctttcaa tttctgtatg
     7741 aaaacgaact ctatcgcgtg cagcggctga aggcacgcaa ccgttcggtg ttgttggagt
     7801 tccagatcaa agacgacgcg ggaacctgga aagcgctgac cgaatgcagc gtggcggaaa
     7861 cgcaagaatg gatcaaccga accctgcatc tggattatga caccttcatc aacgcctctt
     7921 ttttcttgca gggaaaagcg gatgaattta cccaaaagaa ttccacgcaa cgcaaggaga
     7981 tcctggcggg tatcctggga atggacgtat gggagcatta caaagatatc acccgtgaag
     8041 cgcggcgaac ccttgaatct cgcatgaaaa tgatcgaagg ttcgctccac gagatcgaag
     8101 aagaacttgt tcaagaagaa cagcgcaggc aagcgttgga acaggcaaag gcggaattgc
     8161 aggaagcaga gagacagcgc agggagcaac aaaagctgta tgaccaggcg aaaagccttg
     8221 cagagctgat cgcagagcag caaaaacaca tcgatgaatt gaaagaaaga aggaatgttg
     8281 tacaaaccag cgtgcagcga tttgaacagc atatgaaaga acgcctggac gacgaacaaa
     8341 acttggtgca ggtagtggat gctgcgaagg agatcgaaaa aacttttcaa gaacgcgtgg
     8401 ttttgaaaga gaaattgcag gaactcgatg cactctccag ccgattctat acgcttcaac
     8461 agaagcaagc cgaagcagca cagtccattg cggttgagga aagccgcttg aagcaggaat
     8521 tgttctcttt ggaaaatgaa cgttccaaaa tccagcaaat gattgagaat tccctcgaat
     8581 tggagaagga gaaaacccgg ctttcaaacg aactcctgca attacagaca gaagtggaac
     8641 aacttggcgc aataaaaacc caattggacg attgcgttgt gaaaaaaagc gcactgcagg
     8701 cggaaaatgc caccctgcga gcgcatatgc aaaaattgaa agaacgcatg gagcgattga
     8761 aagccgaaac aggctccacc tgtccgttgt gcgggcaacc gctcagcgaa gaacaccgac
     8821 ggcaggtggt cgatgagatc catgcagaag gcaaacagga tgcggaaaat ttccgtaaga
     8881 acgaggagtt gcttagtaaa ttgggggatg agatcattac ctgcgaaaaa caaatcgttg
     8941 cgctgaatga tcaaacgcaa cgattgcgca cgaaggagcg cgaattcggc gcgcttgaaa
     9001 gcgagctgaa gcggctggcg ctggagatcg caagctggaa aagcgacggt gcccccaaaa
     9061 tcaaggcgtt ggaagaaagg ctgaaggaaa gaaaattcgc gcagggggtg cgtgatcaag
     9121 aaactgcgat ctcgttggaa atggaaaagc tgggttatga tgccgcattg catgaatcct
     9181 atcgcaagag ggaacaagcc agccgagatg ccgagcaaaa acatcaggcg gcgttgatcg
     9241 ctgccagccg gttggaagca ttgcgcggag agatcgccat cctgcgttcc caatatcaag
     9301 caggattgga tgaaattgaa atgctgaatt caaagcttga aactttggaa aaccagcatc
     9361 aacaagctgc cagccaggcg cctgatctgg acgaggtgga agggcatcta aaagagttga
     9421 ttgcgatgga gaaccagcag cgcactgtag tgggtggagc catgcagttg ctggcggtga
     9481 tcgagacaca gaaagagcgc aaacagaaaa tccacgatga tctggcagac ctttccaggg
     9541 aacaaacgaa ttttctattg ctggagaagg ctttcgggcg cgatggggta caaacgttga
     9601 tcatggaaga agcattgccg cagatgcagg cgcaagccaa tgacattctg cgaaagctct
     9661 cttcgggttc catgagcgtg cgttttgaaa ccgaacggga atataaagat aagaagcgtg
     9721 atgataaaat gcaggtgctg gatattctca tcagcgatgc cgaaggagtg cagcgcccgt
     9781 atgatctgtt ttcgggcggc gaggcgtttc gtatcaactt tgccatccgg ctggcgcttt
     9841 ccaaagtgct ggcacagcgg gcaggagcac ggctgcagat gctggtcatt gatgagggtt
     9901 ttggcagcca agacgcggaa gggcgccaga agttgatcga agccatcaac ctcatccgcc
     9961 ctgatttcaa aaaaattctg gttatcacgc acctggaaga attgaaggat gctttcccgg
    10021 cacgtattga ggtgagcaag actcggaaag gctcgcaggt agaggtgatg gtgcaatgaa
    10081 agaccaaccc ttgcatattg gagttcaaca gcgcgtgctc ccggtctatc gcatcccttt
    10141 ttttgataag ctggctgagc agtaccgggg caaggtgagc atcttctacg gactgccgcg
    10201 cagtcatgag atgcttgaag ccaatgcggt tcctcagctc gcactgcaca ctcgtgggcg
    10261 gaaccttcac cttttctcgg ggcagttcta cctgtgctgg caatttggtt tgctacagtg
    10321 gctgcaggca tggaagccaa acgtgttgat catggaagcc aacccgcggt atcccaactc
    10381 gcagtgcgca atgggctggg tgaagcgcta tcatcgaaaa gtgatcggat ggggtctcgg
    10441 ttcccccgta gcgggcggtc tgctgggcgg aattcggatg cgccagcgac gaaggttcgt
    10501 gagccaattc gacgctttga tcacctacag ccaacagggg gctcgtgagt atgcccagtt
    10561 gggattcccc gaggagtata tctttgtggc gcccaatgcc gcaacagcca gaccgaccat
    10621 cgtgcccccg gaacgtccgc tacaatatgc gctgaaccga ccgcaggtgc tgttcgtggg
    10681 tcggttgcag gagcgcaagc gggtgaacac cttgatcaaa gcgtgtgcaa aattacccac
    10741 agaactgcaa ccccagttgc acattgtggg ggatggcccg agcaggggca tgctggagca
    10801 attagcgcag caatatttcc cggagacaaa attctacgga gccatgcacg ggaaagaatt
    10861 ggcaacctta tttcaaaaag cagacctttt tgtgctgccc ggcacaggcg gtctggctgt
    10921 acaggaagcc atgagctatg cattgccggt gatggtgggt gaagcggatg gcacacaaag
    10981 cgacctggtt cgccccgaga acggatggat gctggagcgg ggggatgtgg aagaactggc
    11041 gaaaaagatg acggaggcat ttcaagatat tgccgccttg cgaaaaaaag gttctgcatc
    11101 ctacgctatc gtgcggaatg aagtgaacct ggaaaacatg gttgcagtat ttgagaaagc
    11161 gatcgatgca gtgttgaaag gatcgtggag atgaagatcg ttttcgtagc cgacgggcgc
    11221 agcaacatca cccagagctg gttggcagag gtggctggga aggacattga tattttcctg
    11281 atttccacat ttccctgtga aaaaccaaaa gaagcgaagg agatgcggat tctgccggtt
    11341 gcgttttctt ttttggcagg agggcaggta aaacttactg ggcaggagca aaaaaactct
    11401 cgctttcgca aggcattttc cgcatttcgc acgtggctac agaagattcg ctatcgggtt
    11461 ggaccgctca ctctccactt ctataaaagg aactatcttg attttctaga ttccatccaa
    11521 ccggatgagg tgcatgcctt gcgcattccg tttgagggca tgctggcgtc ctttactcct
    11581 gaaaagtatc cgctggtagt gtcgacctgg ggaaacgacc tgacgctgca tgcgggcggt
    11641 tcaaggcgca tggcaaagtg gacgcgcaag gtgctgcagc gcgccaatgc tctgatggcg
    11701 gatgccagcc gcgacctgca cctggctaca gattggggat tgcgcgccgg cgtcccgacc
    11761 ctgatggtgc cgggcaacgg cggattgcac ctggagtatt tcaagcagtt taaccagcag
    11821 atggaatgtg acagtttcaa cccccaaaaa tatcagatca tcaacccgcg cgggtttcgc
    11881 cctgggagcg tgcaccagga gatttttttc aatgccatcc ctggaatctt aaaagagatt
    11941 cctgaggttc aatttgtctg tcccgccatg ggcgggcagg tggaggctga aaaacgggtg
    12001 cgtgaactgg gaatcgaaaa aaaagtgctg ctgctgccct acctgccgca ggcagaatta
    12061 tggcatctgt actatgccag cgacatttac ctttcccttt cgagccatga tgggacgccc
    12121 aataccttcc tggaagcgat cgcctgtggc tgtttcccca ttgttggaga tatcctctcc
    12181 atgcgcgaat ggatcgagga tggaaaaaat ggcatgctgg tggacccaac caatgtggaa
    12241 caggtggtga gcgcggtggt gcgcgccctg caagatgaag ggctgcgcga acgcgcgcag
    12301 gcgctgaatt gggaactggt cagtgagaaa gcagatcgaa tgaaaatccg ctcaaaaatc
    12361 cgtgcttttt attcccatct gctcaccgtt caaccctaga tcaacccagc atctgaccga
    12421 cctctgagat cacttgagcg gtattttgaa agtggatggc gtgtattccg atcttttgtg
    12481 ccgcttcgat attttcgatg agatcatcca caaagatcgt ctcttgcggt tgaacatcca
    12541 aacgagccaa tgccaatcta taaatggctt catcaggttt caccattctc acttcgcatg
    12601 aaatgatcag ctcgtcaaaa agccccgcga tcttaaggtt attttccagc atggggcgca
    12661 agcggttgga atagttgctc aacaacccga cttttttcgt tttgcgaatg gatttgatcc
    12721 tatcaacgag ggggatattc aactggaagg attgataaaa tacctcttca tacagggaaa
    12781 ggagctcctc attgatgccc tgttccttaa acatcgtccg gtagaaagtt ttatggtcaa
    12841 tctctcccag gttgagcttt ctattcagat tgtccttgta gtattttgtc aacgtttcac
    12901 tggaaagacc aagcgattga gcatagtaat tatgacctcg accttcaatc gggatgaaca
    12961 gcaccccgct gaaatcccac aggactgctt taatgctcat accgcttctt tttcttggag
    13021 atgggttgta ttgttgatca cagccagggt ggctttccct tcttcgtcaa tacgtacttc
    13081 actgatgctg catgtgccca gatgataccg ctcgcgcttt ttcattcctt ccccgcaggg
    13141 cagagtagtt aaaatgacgt tgatagccag ggcatgagaa accaccacaa tattcccctg
    13201 gtcttgatgc gcaagaatcc gctgccatgc gttcaacacc ctgctatgca ggtccgctct
    13261 tttttctccg gtgaaaccaa gcatggcatg ccagacagga gcaatgaaaa tgaaaagaaa
    13321 tggaaagcgg gaatacttct tgtacaaatc cggtttcccc tccatgaacc caaatcccat
    13381 ttcgcgcagt ccctccagag gagtgatgga agcagcgggt aacttttcag caatgaagcc
    13441 tgcggtttgc atagctctac cctgcgggct ggaaaaaatg tgcgtgatat tatgcccaag
    13501 cagagcccct gccgcttgct gcgcttccca ttctccaaat ttgctcagag ggctgtcgag
    13561 cgcgccgtgc acatgacctt gctcgatcca ttcggtagtg ccatgccgta ccagataaat
    13621 tgttttcgca accatagcgc tcaattgtac tgtacatgaa agggtaattc aattctttca
    13681 tagttaaagg attgttaaaa aggtaaagag tccgtaagaa aacgaccagc agctcatatg
    13741 cccgtaaata tccgactcgt ttatcagtgc tgtggtgatc acgaggctgc gatcccatac
    13801 ccaattgatg agagaaacgg aaatttgtca tccctttcgg gaatgaaggt ttttgacttg
    13861 taccgttcac ccggctatac tttaaacgta aaaatcccca ggaggttttg tatggctttt
    13921 aaattaacct atgcgaccat gttcaaccca cccgaagaga tgcatacccg ctttgaagat
    13981 gctttgaaag cggtgcgtgc caatcttgga aaagaacatg cgatgatcat taacgggaaa
    14041 gattatttca ccgctgaaaa atttgaggac atcaaccctg ccgataccag cgaggtgctg
    14101 gcgcttttcc aaaaaggaaa tgaaaaagat gcagcggctg ccattgaagc agctcaaaaa
    14161 gcgtttcccc tctggtcgaa gaccccctgg caggagcgcg tgaagttcat gcggcgtgtg
    14221 gcggaactgc tggatgaacg catttatgaa attggcgcca tcgtagcgct ggaagtcggc
    14281 aagaaccgca tggaagcatt gggtgatgtg gcagaaaccg ctgatctgat ccgttatgcc
    14341 tgcgatcaaa tggaagccaa tgacggtttc attaagaaac tggggacgga tccattgagc
    14401 gggtataccg cttcgaatgt atcagtctta cgaccctatg gtgtctggct ggtgatcagc
    14461 cccttcaatt tcccggctgc tctcaccggc ggtccttgcg gcgcggcgtt ggtttctggc
    14521 aatacgttgg tcttcaaacc ggctaccgat actgcctgga cctcccgcaa actagcggaa
    14581 tgcatgcgcg atgcgggatt gccggacggt gttttcaatt ttgtctccgg acccgggcat
    14641 tcgatggggc aggcgctgat cgaaaacccg gcagtggctg gcatcacctt taccggttct
    14701 tatgatgtgg gtatgggcat ctaccgtgat ttcgccaaag gcgaatacgt gcgaccgacc
    14761 atcctggaac tgggcggaaa gaaccccgcc atcgtttcgc gaaatgcgga tattgaagaa
    14821 gcggctgccg gtatcgtgcg ctcggcattc ggacttcagg ggcaaaaatg ttctgcctgc
    14881 tcacgtgtgt atgttgaaaa accggtttac caaaaattgc tggaacgcat gatcgagatc
    14941 accaagaaga tgaccattgg cgacccgacc gaccgcaaca ccttcctggg accggtggtc
    15001 aacagccgtt cttatcaaga ttaccaggat tttttggata agatctatca ttccggtggg
    15061 cgcttcctga cgggtggaaa ggtgttgaaa gatggcgagt atgccaaggg gtacttctgc
    15121 accccgacca ttgtggctga cctgccggtc aggaacagca tctggaaaac cgagatgttc
    15181 gtacccatca ccacggtggc atatgtggac tctttggaag aagccatgca actggcaaat
    15241 gactgcacct atggcctcac cgctggtttc tatggttcag cggaagaaac gagctggttc
    15301 ttcgatacca tccaggcggg tgtcacctat gccaatcgcc cgcagggagc tacgaccggc
    15361 gcatggcccg ggttccaacc atttggtggc tggaaaggtt ctggttctag cggaaagaac
    15421 gcaggtggtt tatactatgt acagttgtac atgcacgaac agatccaaac cttgatcaaa
    15481 aaagcatagc gtcgcttaca tccatgaagc ggttctcata cacctgagga gatgggagcc
    15541 gcttcttttt acaggtggtc atccatgaac aaattatgca gcttaaaaga ggcggtcgct
    15601 gaacatgtaa aagacggtga tctcgtttac gcagccggct ttacccacct catccccttt
    15661 gcggtcgggc atgagatcat ccgccagggg cggcgtgatc tgacgctggc gcgcgccacg
    15721 cccgatctga tctacgatca aatgatcgcg gcggggtgtg ccagaaaggt cattttctcc
    15781 tatctcggca accctggtgt gggttcattg cgcatggtgc gcgcagctat tgaatcggga
    15841 actctggaat gggaagaata ctcccacttc agcatgatta cccggctgac agccggcgca
    15901 tcgggtttgc cttttatgcc tatgaacctc acggctgcac ctgatttgga aaaagtaaac
    15961 cccaatttca aacgcgtgcg ggatcctttt actggaaatg aagtgatggt cgtgcccccc
    16021 ctccatccgg atgtagccat cgtgcacgtg cagcgggcag atgcagaagg gaatgcccac
    16081 atttggggta ttctgggtga gcaaaaggaa gccgcgtttg ccgctcgcaa ggttattctc
    16141 accgcggaag agatcgtgga cgaagcggtg atccgctctg acccgaaccg cacggtcatc
    16201 ccgaatttga tcgtggatgc cgtgtgccat gtgccatact gtgcacatcc atcctatacg
    16261 cagggctatt atgaccgcga caatgatttt tatctgcagt gggatgagat cagcaaatcc
    16321 agagaaaacg ttgatcgctg gttgcaggaa ttcgttttcg acgttcaaga tcacagccgg
    16381 tattggcaga aattgggtga agcgacgcat gcccgtctga gggtcaaaga tcaattcagc
    16441 acccctatca actacggagc gttctagcca gaggaggaag acagcgatga gcgaaagaaa
    16501 gtattcgaaa aatgagctga tgattgtcaa cgccgcccga ctcttgcagg atagcgacct
    16561 ggtatttgtg ggggtgggca ctcccaatct ggcgtgcaac ctggcacgcc gcacgcatgc
    16621 cccaaatctg accatggtat acgaagcggg agtgatcggg gcacagcctg ctcgattgcc
    16681 gctttccatt ggcgatccga cgctggtgag cggagccagc ggcgtgtgca gcatgtatga
    16741 gatcttcagc ctgtatttgc aacgcggaaa tgtggatgtg ggctttctgg gcggagcgca
    16801 gatcgaccgc ttttgcaaca tcaatgctac cgtgatcggt gattattacc atcccaaagt
    16861 gcgcctgccg ggttccggcg gttcgatgga aatcgccgcc tgggcaaatc gctgctatat
    16921 catgaccccg cacgataaac ggcgttttcc tgaaaaagtg gatttctgca cttcagccgg
    16981 tttccttgca ggtcgggcgg aacgagaaaa aagcggggtg cgcggtaagg gtccgcaggc
    17041 agtggtgacc gatctggcta tcatgatgcc ggatgagaag ggtgagctgc aagtgaccgc
    17101 cctgcacccg ggcgtggagt tggaggaggt tcgtgccaac accggttggg atatacaggt
    17161 ggcggctgct gtggagagaa ccgctgaacc gagcgtggaa gaattgcgga tcctccatga
    17221 agaactggac cccagcggga tctatctaca ctaattttcc ggtatttctt tctttggcga
    17281 tctcgtatag atggagagcg agatctcttc cgcgaagaac tggatcccag cgggatctat
    17341 ctacgctaat tttccggtat ttctttcttt gacgatctcg cataagatgg aaagcgcgat
    17401 ctcttccgct ttcttggaac caatatccaa tccaatgggg gcgtgcaaac gagccaggtc
    17461 ttgcgcttgc cagtcagctt ctttcagcgc gttgaaacga ttttggcgtg ttttcaatga
    17521 tcctaaaagt ccaatgtaag cgatcgactt ttccaaaccg cattgcaacg cgggcaggtc
    17581 gatcttttca tcgtggctga tgaccaccag cgcatcctgt tttttcaggt tcaaggatgg
    17641 caggcaatct tgcggccatt tgggaagaat gatatcggca tccgggaagc gcgcttgtgt
    17701 ggcgaacatg gagcgtgggt caacgatgat cacgctgaaa cccaccagtt ttgccatttt
    17761 cgtcaggtag atggcgatct cgctggcacc gacaatgacg atgcggctgg cgggctgaaa
    17821 atagtgaatg tatgccggct gctttttccc atcgaccaat gcgacttcgg cgatatgcga
    17881 tgtgggttga ttccacagtt cttgtatcgc tgtaggggag ggcatgaact gggcggagag
    17941 gtccgtgtac aaaagctttt cccgtcccaa aatgatcttc tcacccgagt ttttttcatc
    18001 caagaagtgg agcaaaaaga acggttcttc gttttctacc tgtgcacgaa ttctctcaaa
    18061 gaaatcaacg gatccttgtg atcttaaaaa tggctcaatc agcacatcaa tctccccccc
    18121 gcaggtcaga cccacttccc aggcgctctg atcgctatag ccaaaatgtt gcaatgtggc
    18181 gtggttgttc ctgatgacat cctgtgcctt ttccaaaatg gcaccttcca cacaaccccg
    18241 gctgactgcc ccttgcattt cagaggcgga ggaaattgcc atcatttcac caaccggcag
    18301 gggggtggtg cctgaggaac gtaccagagt agcaattgcc gcatcctttc cctcagtaaa
    18361 ccagtgccgg atcacatcga ccagtttcgc taatttgttc acatcatcct tcctgttgaa
    18421 aagtcatttg gaaaacagct tatttttggg cgatgatgtc gaaatgatgt tttccaggtg
    18481 gagtattaaa gaattgtatt ttccacgcgc ttaaaagatc gaacacctcc ttttttgtaa
    18541 aagcgtgatg aggcattcct ttttcggtgc cagatcttgg tacaaatgta ttgggctcca
    18601 ccatttccca ttctttgaat ctatctgatt caggatcata ctttagaacg tcaccaatga
    18661 acaaaccatc ttttcgtaag atgcgctcca actctttaaa agtggcttgg atgtctgcga
    18721 agagattgtg ctggatcacc cgaaaagaaa tgatcgcatc aaagctttcg tccttgaaag
    18781 gaagaacctg catatccgcc agtgataacc gggaagggat gctttccttc ctcaaccagg
    18841 tgctggctgc ctgcaaaccc caaccggaaa ggtcagaacc gtatacctca tatccggctt
    18901 tgccaaaata caccagatgc ctgccatttc cacaccccag gtcgaggatg cgttttaccc
    18961 catacttttt gaaaagaata tcggcaagcg aacaaatctt ttcgggttca acaaattgtt
    19021 gcggttcttt tttataagct ttatcccatc cgttgatctt tccggcgatt tttttcattg
    19081 cattcctctt tcgaatctac catggttgca tggtatattt ttgcaggtcg ttgaggtcca
    19141 tatccttctc gctgatggca atcccgtgca cggagattcc caggcgatga acaagatccc
    19201 gctttctgct gcgcaggtgg tgccaccagg gcagcggttt tccttcggct atcctgcgat
    19261 aagcgcacgt ttcgggcagc catgttagct gcctgacgtt ggcggggatc agatcgaggc
    19321 aggtgggcat caggtcatgg cggtgttcat agacccggca gccacaggtt tgcaggtcca
    19381 gatagcggca agcgatgcag gtgaggctgg ttttcgggtc gtaaggagca cgaaccttgt
    19441 acaggcagca gatgccgcac ccgtcacaca acgcttccca ggctgctcga tcgatatggt
    19501 cgagagatgc gagcgtggcg gggttgaacc aggtcatcag cgtgaagcgt ggttgctctg
    19561 atcagccgct gtgttgagca tttgcgccag ttcgcgggct tgctgagagg tgacgtagat
    19621 gttggtttct ccggcataga agatcttctc atcttccggg caatcttctt tgaagcagat
    19681 gcagacatta tcgtcgaatt cgtgatcgcc tttttcgtag aaaatatgca cttgcagcgt
    19741 gccatctaat aatgtgatcg acatacctac ctcttctttt atttctcctt cgcattaatc
    19801 ataccgtaat gacatatttt ctgggtagat ttgtgccctg ataagttttg gaaagaaccc
    19861 aaaaatgctt tagaataaga cggtaagttc aattaaggag tataagaatg ttacttggaa
    19921 tgaagagttt gggaatcgcc gctctggtgt tgatgggatt gttggctggt tgcagcatgc
    19981 cgaccgcaca gcaaagcaca gctacaccat taccagctcc caccgccgat ttgcaaggaa
    20041 aacttgtttt tgcggcaaaa acttcagcaa aagaggatta cgaattgttc ttattggacc
    20101 tgcaaagtgg agatatccag caattaaccg ataatcaatt cgaagatatt gcccctgatt
    20161 tttctcccga tggaacacag atcgcttatt ccgccaaccc gaacggatct tacgatctgt
    20221 tcctgcttga cctggacacg ctgacgatcg aagcgctgct tgaggatgcc acagataaga
    20281 ccgagcccgt ctggtcacct gatggaaaag agatcgccta ccaatccaac ggctacggtg
    20341 ataacgacat ctatatcatc acgctgagcg ataaggttgt acagcgattg accgaagagg
    20401 acgcacaaga cacccaggct agctggcagg cggatggcaa aggcttggct tttgtgtcgg
    20461 atcgggacgg ggattttgag gtgtttgcca tggatatcat ttccaggcag gtaagtcagc
    20521 ttaccaacaa cgaatactat gacgcatcgc cggtttgctc gccagatggt gagaagatcg
    20581 cctttctttc caaacgcgaa ggctcgttca atctctatgt gatgaatgcg gatggaacag
    20641 aagagaccac gctggtgcaa gatcgcgaga ccaaccattc gcccgcttgg tcgcccgatg
    20701 gaagcaaact ggcatttatt tccgattctg aagatcacag cggcatctat atggtgaaga
    20761 ttgagagcgg ggaggtctac ctggtactgg atggatattt ccactacgct ggattatcct
    20821 ggagacagta aatgaaaaag agccgtgcgg tcgctgtagt gcaccccaac tcttggacaa
    20881 aaatcaggta gagaggaaag actgagtacg aaattgaaca gggctcagtc cttttagttt
    20941 agccttgatt ctgcgcttat tgtagtaatc aatgtactca gataacgctt tttcaaagtg
    21001 ctgaaaagag gaaaagtttt ggttgtggta gaactccgac ttgagaatac caaagaagtt
    21061 ttccatgaca ctgttgtcat agcagtttcc cttcctggac atgctctggc ggatgccctt
    21121 gtccttgagc agcttttgat agctgttcat ctgatactgc cagccctggt ctgaatgcag
    21181 gatcaactgg c
//
LOCUS       DAOSDS010000034        20667 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1104_length_20667_cov_4.942170, whole genome shotgun sequence.
ACCESSION   DAOSDS010000034 DAOSDS010000000
VERSION     DAOSDS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 20667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20667
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1104_length_20667_cov_4.942170"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..2019)
                     /locus_tag="PLL88_05630"
     CDS             complement(142..2019)
                     /locus_tag="PLL88_05630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ01081.1"
                     /translation="MTKQNRFTDLLPFLIAIIFLVIYLVQAVQFVKTIEPVMDEGTYL
                     LKGKWYWEGTYQPFEEHGPITNKPPLAFYTLGLSQILFEPGLLSGRYFAVFLSVLLLI
                     GQWLTVRRLAGNWWASFSIALYAISPAWIIYYSRAMTQVVTSLCIVWSLYFFLGRGRT
                     QLQLTLGAALAAMTVMVRQNMLPLFILTLLYILWENGWRKSMVPAISSLLLFIGMNAL
                     YWPATYQFIWQPVFPSFLNKFIAGILHFDLLTGDIGKPFLIRKYHLAYETQVFFDGVR
                     YFFLPFITSIFSLVVLFPRKVFSETEHRKSVYLAINFILLTILHYAYALYENNVLYSF
                     PAYFAFYLPIGVTLIPLIYKDFLKMSSKTRNWLVSIITIATTTGIGLSLYRDISSFFM
                     NWNLPSISQRTLSGPYELWDVLLNRFGISIHLQEFIIPTIVGFLAGLLLWVITIAMRS
                     LFHKKLGNFSFGSVLMLVVLTLGCLFSPTFILAAKGSIATYPSTDIPARYNEAADQMK
                     SIIPENALVYWDGYTPILFLYLPEIRTFPAQLNMEFYYRIGGNTAIIEKQGFWDEELA
                     TRWITEADLLVFSEETYQKRFAVLDASIQSNFRELPGSINPDPYQEGNAAIILERITT
                     K"
     gene            2155..2910
                     /locus_tag="PLL88_05635"
     CDS             2155..2910
                     /locus_tag="PLL88_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ01082.1"
                     /translation="MGMQKKELHSSILDVSAEQIGKYVLLPGDPARVPVLASYFENVH
                     KVSEKWTHVTCTGTLEGQKVSVVSTGMGCPAMAATLEELIRLGAHTFIRVGTSGTIQR
                     KTDPSHLIVTWAAARDERTSLRYQAMSFPAVGDIDVTTALMNAADELAYGYRVGISQS
                     KDAFYGEHDPLRMIAAHALLQKQKTFERSGMLCSEMEVSTLFVISSVRNVRAGGIMML
                     GGSKPQTLEKISKVCSHALELLIRWDKETQEVK"
     gene            2912..3706
                     /locus_tag="PLL88_05640"
     CDS             2912..3706
                     /locus_tag="PLL88_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01083.1"
                     /translation="MAAKNPFSNFAYKLYSFNLAYRKFVCWWKNDVLKKDQYHIGLRK
                     GAGGENVVMVETPAQVRQIAEFYGDFEVLADHREYLSVLVRTPRAKFLAISTGIGTPP
                     IAIGMEELSVIGIQRFLYIGSCKPISSDMKANDLLVVKAAVKEDGTSREYLPGSIPAV
                     ADVKMVNQVRSALSNAKIRFQVGISATVDLDPRHVPAAVPLQAHYDEQIEAFKAGHVL
                     AVDKASAGVYAVATKLHKAACCILRACESEFQLPLSLIASVLEIFS"
     gene            3768..4982
                     /locus_tag="PLL88_05645"
     CDS             3768..4982
                     /locus_tag="PLL88_05645"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOJ01084.1"
                     /translation="MKQIKKLAIPFILASIVMLLAACQTIEDYPAATGTSVPAASTAE
                     NESVGENPTATPITAEVVDWEIEVVAEGLYVPWSIVFTDENRILVSERNGLLREIVDG
                     DLKDRPLYQFEDVAAVDEAGLMGLALDPDYRNNAYIYACYTYREGNTLLNRVVRLVDD
                     PEQLIMDGIVIDQIPSAYYHAGCRLRFAADGTLFITAGDALQPSNAQDLDSLAGKILR
                     VNPDGSIPQDNPYPNSPVYSFGHRNPQGLDWDPRTGLLYATEHGPSGSDGPPGGDEIN
                     LIEAGGNYGWPLVSHEEEGEGLISPLIVFTPAEAPGSGMFYSGTIFPQYKNDFFVGAL
                     RGAGLLRVVISAEDPRLILTVEKIISTVGRVRDVVEGPDGFIYFSTSNLDGRGTASTG
                     DDKIYRLVPLNQ"
     gene            5123..6148
                     /locus_tag="PLL88_05650"
     CDS             5123..6148
                     /locus_tag="PLL88_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOJ01085.1"
                     /translation="MGSKSKNIFRVVGLVVFFALGITILNPYIAKIPESISAIQSLDF
                     GDLGLAVLCEFLRFLGYAVLINAVLGLFKDHVSIWLALIIILASASFGMIAGGMAGSI
                     AASIQWLHERKVKLQAATVASILQNLLSNIAIFLLSLLGVAYLRSMDVLTKPQWNAVL
                     IILCMLVVVLVFSGLFLTKREKSIGFLISVIQWVSKILKKEIDEGKIREQIGEVYEVW
                     DVVLRKKWWRPLLGLSMSFLFDILALYFFFLSAGQPLSFTTLIIGYGLPQLLGKAAFV
                     LPGGIGVVESSMIGLYQKMGISSSVSVIVVLAYRLIAFIIPTLLGFLFVFSLQRVFHK
                     KESVNLR"
     gene            6361..7374
                     /locus_tag="PLL88_05655"
     CDS             6361..7374
                     /locus_tag="PLL88_05655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HOJ01086.1"
                     /translation="MNKNLKKVLRSLLLLVFLGVGVYILLPQFGSIQRSMYVLKNLKY
                     WALFLAILCEFCSYVGAGYLVNSILGLFNERISLWRCVVIVLASSSVGMVAGGMLGSA
                     AASFQWIRNSKAKTQAAALASSLPLLMNNLAILIISLFGVIYLLFLHELTNLQIISFL
                     FILLLLLAVVLAIFLVLYKREKSFPFLLKIMKKVYGFIKRSFNEERIKGQLNEIFAAW
                     DLLLSKGWKKPVLGVLVSYGFDILALFFLFAAGNKWINLGVLLMGYGLPLLLGKATFV
                     IPGGVGVVETTMVALYEKLGMTNAVSVVAVLAYRLIAFWIPSILGFPFTFYLNHKTKE
                     LSS"
     gene            complement(7415..8773)
                     /locus_tag="PLL88_05660"
     CDS             complement(7415..8773)
                     /locus_tag="PLL88_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ01087.1"
                     /translation="MENWIDKSVFYHIYPLGCFGAPPQNDFTSRPVPRLLEVKKWLPH
                     LHDMHINALYLGPVFESTAHGYDTADYFQVDRRLGDSQTLREMINLFHENGIRVILDG
                     VFHHVGRNFFAFEDVQQHGVNSKYCDWFSGLDFSRSSPYGDAFAYDGWNGNFDLVKLN
                     LHNLAVTDHLLRAVEQWIIEFKIDGLRIDTADCVDLSFLQQLFKHCKTHDARFWIMGE
                     IIHGDYSHWVNAHALDSATNYECYKGLYSSHVDHNYFEIAYSLNRQFGDQGIYQHFLP
                     YSFADNHDVNRLISNLTNPAHAYPVYCLLFSMPGIPSIYYGSEWGLTGKRTATDDHML
                     RPHIDPQNVHCNPELKELPGYIAELAQMRSQSSALQTGSYRQLMVANEQFAFLRENEE
                     ESIIVVVNSSQQTVALEIPLEGNYPSALTDLLSGEKYQFQQGQVCIPHLAANSGKIMK
                     IG"
     gene            complement(8817..9383)
                     /locus_tag="PLL88_05665"
     CDS             complement(8817..9383)
                     /locus_tag="PLL88_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ01088.1"
                     /translation="MENQKKILQMGLELFAKRGFDAVGVQEIADAAEITKPTLYHYFG
                     SKEGLLESILRKNFSLLFAELEKSAGYHHDVKNTLEAVVRTYFSFASRHLNFYRFQLS
                     CWFSPPQSTAFNAIKQYGKKQQEIFENLFLQAAQDHGNMRGRHQRYAASFLGIINTYI
                     GLKTSADMELDEPVVSNAVHQFMHGIFS"
     gene            9530..9709
                     /locus_tag="PLL88_05670"
     CDS             9530..9709
                     /locus_tag="PLL88_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01089.1"
                     /translation="MSTVKFYSLKAKKHIVIDSSKVKIVTLKNGRQAAEAIDPETGSK
                     LFKFLSAAELKELKS"
     gene            complement(9859..9984)
                     /locus_tag="PLL88_05675"
     CDS             complement(9859..9984)
                     /locus_tag="PLL88_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01090.1"
                     /translation="MNASANEQVINSKCAWDDAAHSASRRRQGGRKVTDKGSLRS"
     gene            10372..11319
                     /locus_tag="PLL88_05680"
     CDS             10372..11319
                     /locus_tag="PLL88_05680"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOJ01091.1"
                     /translation="MKRYLAAISLILMVLVGCAPGVAASSLTVKDEISSNLQVVEALP
                     TATPAIDAYSIDALAARTYGEGELTVEYLWQRQEDFARYYITYTSDGLTIHGFVNVPD
                     GEGPFPVVIALHGYIPASEYQTLDYSTRYADSIARQGYIVLHPNMRNFPPSDSPGRVR
                     DSLTGYTVDVMNLLELVRQSAGKEGIFEHADMERLGIWGHSLGGGVALRVATLVDEID
                     AIVLYGAVTQRYNDSSIGLPSYDLRVIEAPFSIHHGLEDDTISPAWSESICNTLEELG
                     KEHTCYFYEGQPHTFLRSGSSDALFISRTVDFFGQYLKD"
     gene            11505..12356
                     /locus_tag="PLL88_05685"
     CDS             11505..12356
                     /locus_tag="PLL88_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOJ01092.1"
                     /translation="MISIITDSDSSLPRKLARKYNIKQVPITIQFGEDVYKTDVEIND
                     EQVFARIDKEGKLPTTAAPSPGSFADAYREIFEKEKADAIICFCVSSEISATYSAAKL
                     AAEEFEAKKRIEVVDTRSLTMGQGYMAIVAAETAQAGATVEKIIETAHALRERTHVYG
                     ALATLKYLSMSGRVSHLAAGMAGMLNIKPILAVQNGKLDLIEKVRTRKKAWARVIELV
                     KRDVGSSHIERLCFLHVTAEEDAQEFKQLLQKSVECPKETPFVPLTPGLSVHTGAGLV
                     GLAFVVA"
     gene            12494..12907
                     /locus_tag="PLL88_05690"
     CDS             12494..12907
                     /locus_tag="PLL88_05690"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOJ01093.1"
                     /translation="MFPCICRIHKKNVDLQLGQSALFHGQARLLMILANENGLTHSEV
                     AGELRISPAAATKVIKRMEQDGYVSREADSADERISRVFLQPKGRTVVNEIHETFQNI
                     DRVMFAGFSDQDLQLLRDFLGRVLENLQNNLTKAS"
     gene            13082..14845
                     /locus_tag="PLL88_05695"
     CDS             13082..14845
                     /locus_tag="PLL88_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01094.1"
                     /translation="MVKALAKYIGQYKKHLFMAPILVLLDVVAELSIPILMSRIVDVG
                     IPAGDTRYIMRTGIAMILLALGAIILGVANMKVSATAGQGFGANLRDALFAKVQEFSF
                     ANIDHFSTGSLVTRLTIDVNNLQNTLTMGVRLLLRSPMMLIVAMVLAYRINARLSIII
                     ALAMPVLAIAIALIMTNARKRFTVMQARVDALNSTIQENLIGIRVVKSFVRQNYEKKK
                     FKGANDALTSAAIHAASLVVISMPMMMLVVNAATLAIIKIGGGLVIEGAMGTGELISF
                     ISYVMQILVSIMMLSMIFLMLARAEASGKRIVEVLKTQVDIADPGEMQEGAEKTSREN
                     PQLQFGKIEFRDVSFKYATSGTGENVLSDIRFVVQPGEVVGIVGGTATGKTSLVNLIP
                     RLYDVTAGSVLVDDVDVRDYKLNDLRKDIGMVLQQNTLFSGTIRENLLWGNENASQED
                     LEQVCKDAQAHEFIMSFPDGYDTFLGQGGVNISGGQKQRLYIARAMLKSPKILILDDS
                     TSAVDSATEEKIRRSFYSNFKDTTVLIIAQRISSVSKADKILVLDDGKLVGMGTHTEL
                     MQNNKIYQEINASQQEGVLNA"
     gene            14838..16778
                     /locus_tag="PLL88_05700"
     CDS             14838..16778
                     /locus_tag="PLL88_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01095.1"
                     /translation="MPELKQNNPPERYSQARAERPQGGPGGPARGFEKPKNAGKTLGR
                     ILQYLGKSKGPLLVVVFLLLTNTAASLAGSYFIKPIINTYILPGDVAGLGVALLELAG
                     IYLVGILSAFLQARLMVRIAQKTTNTLRRDLFEKLQTLPLRFFDANTHGELMSRFTND
                     VDNVHMALEQSLPQFLANTLTFFGSMVMMIVLSPTLFLVTGVVLVLMFFITKEITSKS
                     TRYYRDQQKALGTVNGYIEELVGGLKVVKVFTFEQNAIDAFAQYNEEYRRASTNANFY
                     AGSVMPILNNLNNVSYAATAMIGGLLAVAGRFDLGSLAAFLQYSRQFGQPVIQITNQL
                     NNVLAALAGAERVFEVMDQEAEDDNGVVTLVGIRKKEDGLFEVIDGKERTGHWAWKYP
                     QPDGSVQYVELKGDVRFHDVTFGYEPDQVVLKNISLYAKPGQKIAFVGSTGAGKTTVT
                     NLINRFYNTHEGKITFDGIDIKLIKKADVRRSLGMVLQDTHLFTGTVMENIRYGRLDA
                     SDEECIAAAKQVSAHSFIKRLPQGYQTELTGDGANLSQGQRQLLAIARAAVADPPVMV
                     LDEATSSIDTRTERLIEKGMDQLMKGRTVFVIAHRLSTVRNSNAILVIENGEIIERGD
                     HDDLLKQRGRYYQLYTGQYQLT"
     gene            complement(16857..17090)
                     /locus_tag="PLL88_05705"
     CDS             complement(16857..17090)
                     /locus_tag="PLL88_05705"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOJ01096.1"
                     /translation="MTEEFRVNGEEILTKVKELIREGNIRNIIIKNEEGKVLINIPLT
                     VGVVGTVLAPQLAAIGAIAALLARGTIVIEKEE"
     gene            17390..>17839
                     /locus_tag="PLL88_05710"
     CDS             17390..>17839
                     /locus_tag="PLL88_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ01097.1"
                     /translation="MLAVSHLTKSFNLQTLFDNVTFSINPNERVGLIGSNGCGKTTLL
                     RILAGLEEADGGQVHKPANLRIGYLPQGFELPTECAIAQIVNNAVGDVEALNAELVAL
                     SGQLAEQPANQNLAERYDDLLQRIQAAEPRRAARIVAGLGLENIPQDL"
     assembly_gap    17840..17849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17850..19076
                     /locus_tag="PLL88_05715"
     CDS             <17850..19076
                     /locus_tag="PLL88_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOJ01098.1"
                     /translation="AGNLSGGQKTRLSLALLLLQDPELLLLDEPTNHLDIAMLEWLEE
                     WLKNTRCGALIVSHDRMFLDRTVARILELDASSQRLRSYEGNYSAYLEQKEAEIEAQW
                     SAYNDQKEEIRRVQADIARVKEQAKYTERQASANHTGDPEKKFSKDFKKRLAKKVARK
                     AAAREKRLERYKDSDERVEKPKGPRSMRVEFENAPHLGRSVIQMERLSVGFAGFPPLL
                     EHINLQVHASQRIAFTGPNGCGKTTLLRTLAGELAPLAGRVLLGSSVHVGWVSQDLSA
                     LILEKSALEHVWEYFPNQTEARRFLGYYLITGDEVLKPSSQLSFGQRARLMLALLVVR
                     GCNVLLLDEPINHLDIPSRTRFEQALRSFSGAILMVVHDRYFIERFAQEIWQVQDKSI
                     QVGSEVSDTLMAGTVL"
     gene            complement(19205..20248)
                     /locus_tag="PLL88_05720"
     CDS             complement(19205..20248)
                     /locus_tag="PLL88_05720"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOJ01099.1"
                     /translation="MRKPHKLQAGDIVAAVSLSRGTAATFPHRYEAGKKQLEAAFGVQ
                     VVEMPNTLKDADWLAKNPAARAEDLMRAFSDPSVKAIISTIGGDDSIRLLPHLGLEIM
                     RANPKIFMGYSDTTISHMACYRAGLTSFYGPSIMAGFAENGGLFPYMVDSVRRTLFSS
                     EPIGVINPNEEGWTSQRLDWADPSNQAKKRALHACSGWKVLQGTGIHQGHLLGGCFEV
                     LDWLRGTSFFPELQDWQDAILFLDTSEEAPPPVAVQRGLRTYASMGILEKLSGILLGR
                     PGGDTSVQDFELYDAAILQVVRDEQGLTALPIITHMDFGHTDPMFILPYGVKAEIDCN
                     MHTFSILENAVVG"
     gene            complement(20472..>20667)
                     /locus_tag="PLL88_05725"
     CDS             complement(20472..>20667)
                     /locus_tag="PLL88_05725"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOJ01100.1"
                     /translation="LDADINKRVLGLGLYLQKGIPYMIAVAGGENKAKSIHAALSGKY
                     MNILVTDDSAARKILDLSNL"
BASE COUNT         5171 a         4661 c         5423 g         5402 t
ORIGIN      
        1 cccaaatggt caaagccgaa tatctggttc ttgaggaacc atattccagc ctcgattctt
       61 acccgcctga tttgaccctg attttttcac gaggcggata tgccctgttt gaagttcagg
      121 gcgtgagata aattccaacc gctattttgt ggtgatcctt tcgagaatga tggctgcatt
      181 gccttcttga taagggtctg ggttgatact gcctggcaat tctctgaagt tggattggat
      241 cgaggcatcc aggacggcaa accgtttttg gtatgtctct tcggagaaga ccagcaggtc
      301 agcctcagtg atccagcgcg ttgccagttc ctcatcccaa aaaccttgtt tttcaataat
      361 agccgtgtta cccccgatgc gataatagaa ttccatgttc aattgtgcgg ggaatgtgcg
      421 gatttctggc aaatacaaga acaggatggg agtgtagcca tcccaataga ccagcgcatt
      481 ttccggaatg atgcttttca tttgatccgc cgcttcgtta tacctggcgg ggatgtcggt
      541 gcttgggtat gtggcgatgg aacccttggc tgctaagata aatgtgggag aaaacagaca
      601 acccaacgtg agaacaacca gcatcaacac tgagccaaag gagaaattgc ccagcttttt
      661 gtgaaataag ctccgcattg ctatcgtaat cacccataga agcaaaccag ccagaaaacc
      721 aactattgtt gggatgatga attcctggag gtgtatggag atgccgaatc tgttcagcag
      781 cacatcccat aattcatacg gtcccgagag ggtgcgctgg ctgatgctcg gtaaattcca
      841 attcatgaag aacgaggaga tgtctcgata taaactcaag ccgataccgg ttgtggttgc
      901 gatggtaatg atgcttacca gccagttccg agtttttgag gacatcttta aaaaatcctt
      961 ataaatcagg ggaatcaatg tgacaccaat gggcaaatag aaggcgaaat atgccgggaa
     1021 actgtataaa acattgtttt cgtacagggc gtaggcataa tgcagaatgg tgagcaagat
     1081 gaaatttatc gccaggtaga cgctttttct gtgctctgtt tctgagaaaa cttttcttgg
     1141 gaacaaaacg accaaagaaa atatggacgt gataaacgga agaaaaaaat accgcacccc
     1201 atcaaaaaag acctgtgttt catatgccag gtggtacttt cgtatgagaa agggttttcc
     1261 gatgtcgccc gtcaggaggt caaagtgtag aattccggcg atgaacttgt tgaggaaaga
     1321 gggaaaaact ggttgccaga tgaattgata ggtggctggc cagtagagcg cgttcattcc
     1381 gatgaagagc aaaaggctgc tgattgccgg caccatgctc ttccgccatc cattttccca
     1441 cagaatatac aggagtgtaa gaatgaagag aggtagcatg ttctgtctga ccatcacggt
     1501 catggcagct agagcagctc caagggtaag ctgaagttgc gttctgcctc tgccgagaaa
     1561 gaaatacagg ctccacacga tgcataacga cgtaaccacc tgggtcatag cccgtgagta
     1621 atagatgatc caagcgggtg agatggcata aagcgcaatg gaaaaagaag cccaccagtt
     1681 tccagccagg cgccgaacgg tgagccattg accgatcaat aacagaacgc tgaggaaaac
     1741 tgcaaaatat cttcctgaca gaagacctgg ttcaaaaagg atctgcgata gaccgagcgt
     1801 gtaaaaagcc agaggaggtt tattggtgat cggtccatgc tcttcaaagg gttggtaagt
     1861 accttcccaa taccattttc ccttcaacag atatgtgccc tcatccatta caggttcgat
     1921 cgttttcacg aattgaactg cctgtacgag ataaattacc agaaatatga tggcgataag
     1981 aaaagggagg aggtctgtaa atctgttttg ttttgtcatg gtcaacctgc gatcgctctg
     2041 ccgggcatct ttgtcgcatg cgatcgatgt gtatcatcca tgcgttgaag tgtttctttc
     2101 gacgctttcc atatataatg ataattcatt tttcttcagg agtgatttgt gcttatgggt
     2161 atgcagaaaa aagaactaca ttcatcaatc ttggatgttt cagcagagca aatcgggaag
     2221 tatgtgctcc ttcccggaga cccggcgcgc gtgcctgttc tggcgagcta ctttgagaat
     2281 gttcacaaag tatcggaaaa atggacgcat gttacttgca cgggtaccct ggagggtcaa
     2341 aaagtgtcgg ttgtatccac gggaatgggc tgcccggcga tggctgccac tctggaagaa
     2401 ctgattcggt tgggcgcgca cactttcatt cgcgttggca cctcaggcac catacaacga
     2461 aaaaccgatc cttcgcatct cattgttaca tgggctgctg cgagggatga acgcacatcg
     2521 ttgcgttatc aagccatgtc ttttcctgcg gtaggggata ttgatgtaac aaccgcattg
     2581 atgaatgcgg ctgatgagct tgcttatggg taccgggttg gcatctctca atccaaagat
     2641 gccttctatg gagaacatga tcccttgagg atgatcgcag cgcacgcgtt gctgcaaaag
     2701 caaaaaacgt tcgagcgctc gggtatgctt tgcagcgaga tggaagtttc taccctcttt
     2761 gtcatatcct cagtccgcaa tgtgcgggct ggggggatca tgatgctcgg agggagcaaa
     2821 cctcaaaccc tggagaagat ctcaaaagtt tgttcacatg cattggaatt attgattcgc
     2881 tgggataagg aaacacaaga ggttaagtaa aatggctgcg aagaatcctt tttcaaactt
     2941 tgcctataaa ttgtattcgt tcaatcttgc atatcgaaaa ttcgtctgct ggtggaagaa
     3001 tgatgttctc aagaaagacc agtaccacat cggtttacgt aagggcgcag gcggggagaa
     3061 tgtggtgatg gttgaaacgc ccgcccaggt tcgccagatc gctgaatttt atggtgattt
     3121 tgaggtgctg gctgatcatc gtgaatacct gagcgtactc gttagaactc cccgagcaaa
     3181 atttcttgcg atttcaaccg gcattggcac cccgcccatc gcgatcggga tggaagaatt
     3241 gtccgtcatt ggaattcagc gattcctcta tatcggttcc tgtaaaccga tatcttcaga
     3301 catgaaagcg aacgacctgt tggtcgtgaa ggctgctgtt aaggaagatg gcacgagtcg
     3361 cgagtattta ccgggcagca tccctgccgt ggcggatgtg aaaatggtca atcaggtgcg
     3421 gtctgcgttg tcgaatgcca aaatccgatt tcaggttgga atcagcgcca ctgttgatct
     3481 tgacccccgg catgtccccg ctgccgtccc cctgcaggct cactatgacg aacaaattga
     3541 agcgttcaaa gccggtcatg ttctagccgt tgataaggct tccgcaggcg tgtatgcggt
     3601 ggctacgaag ctccacaaag ctgcgtgctg tattttgcga gcgtgcgagt ccgaatttca
     3661 attgcccctc tcattgatag ccagcgtgct ggaaattttt tcataagagt cgaaatcact
     3721 tcattgctct ggttttattt tttgtactac cttgatatac tgagaatatg aaacagataa
     3781 aaaaactggc gatccctttt attctggctt cgattgttat gcttctggcg gcatgccaaa
     3841 cgattgaaga ttatccggca gccaccggaa cctctgtgcc ggcagcgtca acggcagaga
     3901 atgagagcgt cggtgaaaat cctaccgcca cacccattac cgcagaagtg gttgattggg
     3961 agatcgaggt tgtggctgag ggtctctatg tgccgtggag cattgtattt acggatgaaa
     4021 accgcattct cgtatctgag cggaatggct tgctgcgaga gatcgtagat ggtgatctga
     4081 aagatcgccc gctatatcaa tttgaggatg tggctgcagt ggatgaggcg gggctgatgg
     4141 gtctggcact ggacccggat taccgcaaca atgcctatat ttacgcttgc tatacctatc
     4201 gggaagggaa cacgttgctc aatcgcgttg tgcggctggt tgacgaccct gaacaactaa
     4261 taatggatgg aatcgtcatt gatcagatac cctccgcgta ttaccatgct ggctgccgtc
     4321 tgcgttttgc ggcggatgga accctgttca tcaccgccgg cgatgctctg cagccgagca
     4381 atgcacaaga tttggattct ctggctggaa agatcctgcg agtaaatccg gatggaagca
     4441 tcccgcagga taatccctat cccaattcgc cggtctatag tttcggtcat cgcaatccac
     4501 aggggttgga ttgggatcct cgaacgggtt tgttgtatgc cactgagcac ggaccgtcag
     4561 gatcagatgg accccccggc ggggatgaga ttaacctcat tgaagcagga ggcaattatg
     4621 gttggccgct tgtttcacat gaagaagaag gtgaagggct catctctccg ctgatcgtct
     4681 tcacaccggc tgaagcccct ggatcgggca tgttctatag tggaacgatt tttccgcagt
     4741 acaaaaatga tttcttcgtc ggtgctttgc gcggggcggg attgttgcgg gtcgtcattt
     4801 cagcggagga cccgcgtttg atccttacgg tcgaaaaaat catatccact gttggtcggg
     4861 tgagggatgt ggttgaggga ccggatgggt tcatctattt ctctaccagc aacctggatg
     4921 gtcgagggac tgcatcaacg ggagacgata agatttatcg actcgttccc ctgaaccagt
     4981 gagtgttcac atgaggcaat gacccttcat aaccagctca tttttctgtg ccggtgtatt
     5041 tcccaaaaac tttgctgaat tgtaccaatt ccattccgca ggggtcgatc ccatataatt
     5101 gtcgctgtca gggaggttga caatgggttc aaaatcgaaa aacatctttc gcgtggtcgg
     5161 attggtcgta ttcttcgccc ttgggataac catattgaat ccttacattg cgaagatacc
     5221 tgaatcgatc agcgctatcc agagcctgga ttttggcgac ctggggctgg cagttctctg
     5281 cgaattctta cgattcttgg gttatgccgt tttgatcaat gcggttctgg gattgttcaa
     5341 agaccatgtc tcaatctggc tggcgctgat aatcattttg gcatcggcga gttttggaat
     5401 gatcgctggg gggatggccg gttccattgc tgccagtatc caatggctgc acgaaagaaa
     5461 agtgaagttg caagcagcca ccgttgccag catccttcaa aaccttttga gtaatatcgc
     5521 gatatttttg ctctctttat tgggtgttgc ctatctcagg tccatggatg tcttgacaaa
     5581 accgcaatgg aatgcagtgc tgatcatcct gtgcatgctt gttgtggtgt tggtcttttc
     5641 aggcttgttt ctcaccaaac gggaaaaatc gatcgggttc ctgatttcgg tgatccaatg
     5701 ggtcagcaag attttgaaaa aagagattga tgaaggaaag atacgggaac agatcggtga
     5761 agtatatgag gtttgggatg tggtcttgcg caaaaaatgg tggcgccccc tgctggggct
     5821 ttctatgagc tttctgttcg acatcctagc tctctatttc ttttttctct ccgcggggca
     5881 acccctttca ttcacaaccc tcatcatcgg atatggcttg ccgcagcttc tagggaaagc
     5941 ggcgtttgtt ttaccaggtg gtattggtgt ggtggaaagc tcgatgatcg gtctctatca
     6001 aaaaatgggg atttcaagtt cggtatcagt gattgtggtt ttggcatatc gcttgatcgc
     6061 ttttataatt ccaacattgc ttggatttct tttcgttttt tccttacaaa gggtatttca
     6121 taaaaaagaa agcgtgaatt tgaggtagga aaaactgata aaatgccgtt ggagttgaaa
     6181 tttcctgtgg atctgccgtt tgatgctcgg agcaagatgc gtgacaaaaa ggtaattttt
     6241 cacttaaaat aaggtagttt ggagcggcaa gcaagagatg gaagaaaaac agacattatt
     6301 ttggtatggg cttaattgca gtttgttatc tctgcaagta agttttcgaa ggagtgttgt
     6361 ttgaacaaaa atttaaagaa ggttcttcgc tctctgcttc tccttgtttt tcttggggtt
     6421 ggcgtgtaca ttttgcttcc ccaattcggt tcgatccaga gatcgatgta tgttctgaaa
     6481 aatttaaaat attgggcttt atttctggcg attctgtgtg aattttgtag ttatgtgggt
     6541 gctggatatt tggtcaattc catactgggg ttgttcaatg agcgcatttc gctctggcgc
     6601 tgcgtggtca ttgtgcttgc ttcttccagc gttgggatgg ttgccggggg aatgctgggg
     6661 tctgcggctg ccagctttca gtggatacgg aacagcaagg cgaaaacgca agctgctgcc
     6721 ctagccagca gcctgccgtt attgatgaat aacttggcaa ttctgatcat ctcacttttt
     6781 ggtgtcattt atctcctttt tttgcatgag cttaccaatc tgcagatcat cagtttcttg
     6841 tttattctgt tgcttctcct tgccgttgtt ttggcgattt ttctcgtgct gtacaagcgt
     6901 gaaaaatcct ttcccttttt gttgaaaatc atgaagaagg tctatggttt catcaaacgt
     6961 tcattcaatg aggagaggat aaagggtcaa ctaaacgaaa tatttgctgc ctgggatctg
     7021 ctgttgagta aagggtggaa aaaacccgtg ttgggcgtgt tagtgagtta cgggtttgac
     7081 atcctggcgc tctttttcct gtttgcggcg ggaaataaat ggattaatct gggagtgctg
     7141 ctgatgggtt atgggctgcc attgctcctg gggaaagcga cctttgtcat tcccgggggg
     7201 gtaggggtcg ttgagacaac catggtggca ttatatgaaa aactggggat gaccaatgcg
     7261 gtttcggtgg ttgcagtgtt ggcatatcgc ttgatcgctt tctggatccc cagcattttg
     7321 gggttcccgt tcacctttta tttgaaccac aagaccaaag agctctcctc ttgagcaacg
     7381 gtgcgcagca attttccgat tatgctctgc tcgcttaacc gattttcatg atcttgccag
     7441 aatttgcagc aaggtgaggg atgcacacct gcccctgttg gaactgatat ttctccccgc
     7501 tcaacaggtc tgttaaagcg gaagggtaat ttccttcgag cgggatttca agcgccacgg
     7561 tctgctggct ggaattgacg accacgatga tgctttcttc ttcgttttct ctcaaaaacg
     7621 caaattgctc attcgcaacc attaactggc ggtatgaacc agtttgcaat gcggaggatt
     7681 ggctccgcat ctgcgccagt tccgcgatat aacccggaag ctcttttagc tcaggattgc
     7741 agtgaacatt ttggggatcg atgtgcggtc gaagcatgtg gtcgtcggta gctgttcttt
     7801 ttccggtcag cccccactcg cttccgtaat agattgatgg aatgccgggc atgctgaata
     7861 gcaggcaata taccggatac gcatgagcgg gattggttaa attgcttatt agccggttca
     7921 catcatgatt gtctgcgaat gaatagggca gaaaatgctg gtaaatgccc tgatcgccaa
     7981 attggcgatt caacgaatac gcgatctcaa agtaattgtg gtccacgtga ctggaataca
     8041 accctttgta acattcatag ttcgtcgccg aatccagggc atgtgcgttg acccaatgag
     8101 aataatcccc atgaatgatc tcgcccatga tccagaaacg cgcgtcatga gttttgcagt
     8161 gcttgaaaag ctgttgaaga aatgaaagat cgacacaatc tgcggtgtcg atgcgcaagc
     8221 catcgatctt gaattcgatt atccattgct caactgcgcg caagagatga tcggtgacag
     8281 ccaggttgtg caaatttagt ttgaccaggt caaaattccc attccaacca tcgtacgcga
     8341 aggcgtctcc gtaggggctt gatcttgaaa aatcgagccc tgaaaaccaa tcgcaatatt
     8401 tcgaatttac tccatgctgc tgtacgtctt cgaatgcgaa gaaatttcga ccgacgtgat
     8461 ggaaaacccc atccagaata acccggatgc cattctcatg gaacaggttg atcatttcgc
     8521 gcagggtttg actatctccc agccggcggt cgacctggaa gtaatcggct gtgtcgtagc
     8581 cgtgggcggt ggattcaaaa accggaccaa ggtacagcgc gttgatgtgc atatcgtgaa
     8641 gatggggcaa ccactttttc acttccaaca gccggggaac cgggcgcgag gtgaaatcat
     8701 tttggggagg tgcgccaaaa caacccagag ggtaaatgtg ataaaaaaca cttttatcga
     8761 tccaattctc catatcgttc cttaagtcca tattggtagt gggttacgtg gaaaaattat
     8821 gaaaaaatgc catgcatgaa ttgatgaacg gcattgctga caactggttc gtcgagctcc
     8881 atatccgcgc tggttttcaa gccgatatag gtattgatga ttcccaaaaa tgatgcggca
     8941 tatcgctgat gcctgccacg catatttccg tgatcttgag ctgcctgtag gaagagattt
     9001 tcgaagattt cctgttgttt ctttccgtat tgcttgatcg cgttgaacgc agtgctttgc
     9061 gggggagaga accagcagga aagctgaaag cgataaaaat tgaggtgtct gctggcaaaa
     9121 gaaaagtatg ttcgcacaac cgcttcgagc gtgtttttga catcgtgatg atagccagcg
     9181 cttttttcca actcggcaaa aaggagtgaa aagttcttgc gcagaatgct ttccagcaga
     9241 ccttccttgc tgccgaaata gtgatagagg gtcggtttgg tgatctccgc ggcgtctgcg
     9301 atctcctgga cgcccactgc atcaaacccg cgtttggcga aaagctctaa ccccatctgt
     9361 aggatctttt tttggttttc catgcgtgag agtataccgt tcggtatacc caatgtcaag
     9421 aatactgtgt ataatcaaaa tattgatctg cccattcgtt ggggttatca tttcttgatt
     9481 gatttgtcgc gccgtcaccg ccgtcgcaga aaaaattcag gaggatgaaa tgtcaacagt
     9541 gaaattctat tcattaaagg cgaaaaagca tatagttatc gacagcagca aagtcaagat
     9601 cgtgacattg aaaaacgggc gacaggctgc tgaagcgatc gatccggaaa ctggcagtaa
     9661 gttgttcaaa ttcctatcag ccgcagagtt gaaagaattg aaatcataag cttacttctt
     9721 atcatttctt tataaaaagt tcgagttact ttagcgctca cttctggaga tagcttttat
     9781 ctcgtagaga aagagggcgt ctatttatcc tgcaaagcgt gaatttccag ccctgcatgg
     9841 attttctcca tgctgcgctc atgagcgtag cgatcctttg tcggtcactt tcctgccgcc
     9901 ctgccgtcga cgcgatgcgg aatgtgctgc atcatcccag gcgcactttg aattgatgac
     9961 ctgctcattt gccgaggcgt tcatttttcc cggcgctgcg gaaacgtatt gataaaaaca
    10021 tcgctttgga tccgtttggt ggttatggac atttgacgat ttttcgagcg gggatgtgaa
    10081 attgtcgctc ctggttccga atggaaagaa atatcatcaa aatacatgag tattcttgat
    10141 agaagaagaa aaagatcgtt gcacgactgg catgctgtaa gcagtgcggc gacctgcaac
    10201 catggcttct tgatcccccg accgtagaca attgcgcttg tctgaagctg cgtatacaga
    10261 gcagctatct aatggattgt taataggaac agggaaggac aattcatata atcatgctta
    10321 ataaacagaa aaaagaaaag ttaattttac tgttcgatag gaaaagaaaa aatgaaacgc
    10381 tatttagctg caatcagctt gatcttgatg gtgctggtgg gctgtgcgcc cggcgtggct
    10441 gcaagcagcc ttaccgtgaa agatgaaatc tcctccaatt tgcaggttgt agaagcgctt
    10501 cctactgcca ccccggcgat tgacgcgtat tctattgacg ccctggctgc tcgaacctac
    10561 ggagagggtg aactaacggt cgagtatctg tggcaaaggc aggaagattt tgcccgctac
    10621 tatatcacct ataccagcga tggtttgacc attcacggat ttgtcaatgt ccctgatgga
    10681 gagggcccgt tcccggtggt gatcgctctg catggttaca taccagcctc tgagtatcag
    10741 accctggatt actccacccg ctatgccgat tccatagccc gccagggtta tatcgtgctg
    10801 catccaaaca tgcgcaactt tccgccttct gatagtcccg gacgcgtgcg cgattcgctc
    10861 accggctata cggtggatgt catgaatctg ttggagcttg tgcgccagag tgcaggcaaa
    10921 gagggtatct tcgagcatgc ggatatggaa cgcctgggga tttgggggca cagcctggga
    10981 ggaggggttg cgctgcgcgt agcgaccctg gtggatgaga tcgatgcgat cgtgctgtat
    11041 ggcgcggtaa cacaacgtta caacgactct tctatcgggt tgccctctta cgacctgcgc
    11101 gtgatcgaag cgccgttcag cattcaccat ggtctggaag acgatacgat ctcgcctgcc
    11161 tggtctgagt ccatttgcaa tacactagaa gaactgggta aagagcatac gtgctatttc
    11221 tacgaagggc aaccgcatac cttcctccgc tcggggtcca gtgatgccct gttcatctca
    11281 agaacggtgg acttttttgg gcagtatttg aaagattgat tgggagatat cgcgtttctg
    11341 aagaaccgct gtttctgata aaaatgggtg taaagacgca cccattttta ttttggcagc
    11401 gcttgtcctt ctggttcgaa aaatagttgt gaaacttcca taaatatgct agcataactc
    11461 attgaggaat ttttgttaat tcattataaa agggagtacc cgtcatgatt tcgattatta
    11521 cagattctga ttccagcttg ccccgcaaac tagcgcgaaa atataacata aagcaggttc
    11581 ccatcacgat ccaatttggt gaagatgtct ataaaacaga cgttgaaatt aacgacgagc
    11641 aggtttttgc aaggattgat aaagaaggaa aactgcctac caccgccgct ccgtcaccag
    11701 gcagctttgc cgatgcatat cgagaaatat ttgagaaaga gaaagctgat gccatcattt
    11761 gcttttgcgt aagcagtgag atcagtgcaa cctatagcgc agcgaaactg gcagcagaag
    11821 aatttgaggc aaagaaacgc atagaagttg tggacacccg cagtctgacc atggggcagg
    11881 ggtatatggc aatcgttgcg gctgaaacag cccaggcggg cgcaactgtt gaaaaaatca
    11941 tcgaaaccgc gcatgccttg cgtgagcgga cgcacgtata tggcgctctc gcaacgttga
    12001 aatacctctc gatgagcggg cgggtaagcc acctcgcagc gggaatggca ggcatgctga
    12061 acattaaacc gatcctcgcg gttcagaatg gaaagttgga tctgatcgag aaggtgcgca
    12121 cccgaaaaaa agcctgggcg cgcgtcattg aattggtgaa gcgcgatgtc ggttccagcc
    12181 atattgaacg cctgtgtttt ctgcatgtca ccgccgaaga ggacgctcag gaattcaagc
    12241 aactcttgca gaaaagcgtg gagtgcccga aagaaacgcc cttcgtccct ttaacccccg
    12301 ggctttctgt tcacaccgga gcaggcttgg tgggtctggc atttgtggtt gcgtaaaatc
    12361 atcccgattt ttctattgcc gtaatgcctc aatgaaaaag ttactttggt agtaacgttg
    12421 cgcataaata ctaaactagt ttataattaa ctgtattatg aataagctca cgaactatga
    12481 atccgtagga aggatgttcc cctgtatttg ccgcattcat aaaaagaatg tcgatctgca
    12541 gcttgggcaa agcgcccttt tccatgggca ggcgagattg ttgatgatcc ttgcgaacga
    12601 aaatggtctc acccattcag aggtggctgg cgagctgcgc atctcgcctg ccgccgccac
    12661 caaggttatc aaacgcatgg agcaagatgg ttacgtttcg cgcgaggcgg atagcgcgga
    12721 tgaacgaatt tcaagagtct ttctccagcc caaggggcgg accgttgtca atgagatcca
    12781 tgaaacgttt caaaatattg accgggtcat gttcgctggt ttttccgatc aggatttgca
    12841 attgctccgt gattttcttg gcagggtgct ggaaaatctt caaaacaacc tgaccaaagc
    12901 ttcttgatca agaccattga ttttcttttc gaaaatattt ttctattgac aaaagaacgt
    12961 ttcccatgga taatgcgtaa ccagctaata ataaacaagt taaataatat tccgaacgat
    13021 agattcgata gtccgtcttt caacgttttg ttcatggcag gacatttcat ggaggtagta
    13081 tttggttaaa gcattagcaa aatacatcgg gcagtataaa aagcatttgt tcatggcgcc
    13141 aatccttgtg ctgttggatg tggtggctga attgagcatt cctatactca tgtccagaat
    13201 agttgatgtg ggaatccctg caggcgatac ccgttacata atgcgaacgg gcatagccat
    13261 gatcctcctt gcgctgggtg caattattct gggggtagca aatatgaaag tttctgccac
    13321 tgccgggcag ggattcggag ccaacctgcg cgatgcgctg tttgccaaag tccaggaatt
    13381 ttcatttgct aatatcgacc atttcagcac cggctcgctg gtgacgcgat taaccattga
    13441 tgtgaacaac ctgcaaaaca cgctcaccat gggagtccgt ctcttgctgc gttctcctat
    13501 gatgctgatc gttgcgatgg tgttggcgta tcgtatcaat gcgcgcttgt ccatcattat
    13561 cgctttagct atgcctgttc ttgctatcgc gattgctttg atcatgacca atgccagaaa
    13621 gcgattcacg gttatgcaag cccgtgtgga tgctctgaac agtaccatcc aggagaacct
    13681 gatcggtatt cgtgtggtga aatcgttcgt tcgacagaat tatgagaaaa agaaattcaa
    13741 gggggcgaac gatgcgctga ccagtgctgc catccatgct gccagtctgg ttgtgatcag
    13801 catgccgatg atgatgctgg tggtgaacgc agccacgttg gcgatcatca agatcggtgg
    13861 tggattggtc atagaaggag ccatggggac gggcgaactg atcagtttca tcagctatgt
    13921 gatgcagatt ctcgtttcga tcatgatgct ttccatgatt ttcttgatgc ttgccagggc
    13981 tgaagccagc ggcaagcgca tcgtcgaagt tctcaaaacg caggtggata tagccgatcc
    14041 gggtgaaatg caagaaggcg ctgaaaagac ctcaagagaa aatccgcagc tacaattcgg
    14101 aaagatcgag ttcagggatg tgtcatttaa atatgccaca tcgggtacgg gtgagaacgt
    14161 tctgtcggat attcgttttg tcgtacagcc tggggaggtg gttggaattg tgggaggcac
    14221 cgcgaccgga aaaacctctc tggtgaatct cattccgcgc ctgtacgacg tgactgcggg
    14281 gtcggtgttg gtggatgatg tggatgtgcg cgattataaa ttgaatgatc tgcgaaaaga
    14341 tattgggatg gttctacagc agaacaccct tttcagtggc accattcgcg agaatttatt
    14401 atggggaaat gaaaatgcgt cacaagaaga tttggaacag gtgtgcaaag atgctcaggc
    14461 gcacgaattc attatgtctt tccctgatgg atacgacaca tttttggggc agggtggggt
    14521 caatatttcc ggtgggcaaa aacagcgcct gtatattgcg cgcgccatgt taaaaagccc
    14581 caagatcctg attttggatg acagcaccag tgcggtggat tctgccactg aagaaaagat
    14641 caggagatcc ttttattcca attttaaaga tacaacggtg ttgatcatcg cccaaaggat
    14701 cagctcggtg agcaaagcgg ataagatcct ggtgctggat gatggaaaac tggtgggaat
    14761 gggcacgcat acagaattga tgcagaacaa caagatctac caggagatca acgcttcgca
    14821 acaggaaggg gtgctcaatg cctgagttga aacaaaataa cccgccagaa cgctattccc
    14881 aggcaagagc ggagcgaccg cagggaggtc cggggggacc tgccagaggt tttgaaaaac
    14941 cgaagaacgc gggaaaaaca ttgggcagga tcttgcagta tttgggaaag agcaaagggc
    15001 cgctgctggt ggtggtcttt cttcttctga cgaataccgc ggcgtctttg gctggcagct
    15061 acttcatcaa gcccatcatc aatacctata ttttgcctgg tgatgtggct ggtttgggtg
    15121 tggcattgtt ggaattggct ggcatttacc tggttggtat tctgtccgct ttcctgcaag
    15181 cccgtttgat ggtgcgcata gcccaaaaaa cgacgaacac cttgcggcgc gacctgttcg
    15241 aaaaattgca aaccttaccg ctgcgcttct ttgatgccaa tacacacgga gagttgatga
    15301 gccgtttcac caacgatgtg gataatgtgc atatggcgct tgaacagagc cttccccaat
    15361 ttctggcgaa cacgctgacc tttttcggct ccatggtcat gatgatcgtt ctaagcccca
    15421 cgttgttcct tgtcactggt gtcgtgttgg tgctgatgtt ctttattacg aaagagataa
    15481 catccaagag tacccgctat tatcgtgacc aacaaaaagc gctgggtacg gtgaatggat
    15541 atatcgaaga actggtgggg ggattgaaag tggtgaaggt gttcacgttc gaacaaaatg
    15601 cgattgacgc gttcgcccaa tataatgagg agtatcgcag ggcatccacc aacgccaatt
    15661 tttatgctgg ctcggtcatg cccattctga acaatctgaa caatgtttct tacgccgcta
    15721 ctgcaatgat tggtggtttg ttggcagtgg ctggcagatt tgacctcggt tctctggcgg
    15781 catttctgca atattcacgg cagttcggtc agccggtgat ccagatcacc aatcagttga
    15841 acaacgtgtt ggcagctttg gctggggcgg agcgcgtctt cgaggtgatg gatcaggaag
    15901 ctgaagatga caatggggtg gttaccctgg ttgggatccg gaaaaaagag gatggacttt
    15961 tcgaggtcat cgatggaaaa gagcggaccg gacattgggc atggaaatat cctcaaccag
    16021 acgggagcgt gcaatatgtt gaactgaagg gcgatgtgcg ctttcatgat gtgacctttg
    16081 gttatgaacc tgaccaggtg gttttgaaga acatatccct gtatgccaag cccggtcaaa
    16141 agatcgcttt tgtgggctcc accggagccg gcaagaccac ggtcaccaac ctgatcaacc
    16201 gcttctataa cactcatgaa ggaaagatca ccttcgacgg catcgatatt aagctcatta
    16261 aaaaggcgga tgtgcgccgc tcgctgggca tggtgttgca agacacgcat ttgttcacag
    16321 gcaccgtgat ggaaaatatt cgctacggtc ggttggacgc cagcgatgag gaatgtatcg
    16381 cagccgccaa acaggtcagc gcgcactcct tcatcaagcg cctgccgcaa ggctaccaga
    16441 cggagttgac cggtgatggc gccaaccttt cgcaggggca gcgccagttg ctagccattg
    16501 cccgtgccgc cgtggctgac ccgcctgtca tggtgctgga tgaagccact agttccatcg
    16561 atacccgcac cgaacgattg atcgagaagg gcatggatca gctcatgaag ggacgcaccg
    16621 tgtttgtgat tgcccatcgc ctttccaccg tgcgcaactc gaacgctatc ctggtcatcg
    16681 agaatggcga gatcatcgag cgcggtgacc atgatgatct gctcaagcaa agaggcaggt
    16741 attaccagct ttacaccggg caatatcaat taacctgaca aaaaacgagg aacaggtact
    16801 gtgttcctcg tttttcttgt catccgattc acgaagttct gcgggaatga cgaacgctat
    16861 tcttcttttt caatcacgat cgtgccacgc gccagcagtg cggcgatggc accgatggca
    16921 gccaactgtg gggcgagcac ggtgcccacc acccccaccg taagtggaat attgatcaga
    16981 acctttcctt cttcgttctt gatgatgata ttgcggatgt tgccctcgcg aatcagttcc
    17041 ttgaccttgg tcaggatctc ttcgccgttc actcgaaatt cctcagtcat tttattgctc
    17101 ctgttaaatg taaatggctt tttcactatg catacggatt tggataccaa tccgttgctt
    17161 tctacaatga aagaaaccga agcggaattg acgaattttg catgcttttc ttgacacccc
    17221 gcacatttgt cagtatgatg gattgtgtct tagaaaaaga gaacgcaaaa aatgattgac
    17281 ggcaagtagt ttatcacgca cctcccgcac aactctccac agggcagagc gggcgcccga
    17341 tttatgatcg ggtgattcgg tatttttctc accggaatag gaggcattca tgcttgccgt
    17401 ttctcattta acaaaaagtt ttaacctaca aacattattt gacaacgtca cattttcgat
    17461 caatcccaat gagcgggttg gtcttatagg atcgaacggc tgtggcaaga ccacgctgct
    17521 gcgcattctt gccgggctgg aagaagcgga tggcggtcag gtgcacaaac cagccaattt
    17581 gcgcatcggc tacctgccgc agggttttga acttcccaca gaatgtgcca ttgcacagat
    17641 tgtgaacaat gcggttgggg atgtggaggc tctcaacgct gagttggtcg cgctatccgg
    17701 gcaactggcg gagcagcctg cgaaccaaaa tctggcagag agatatgacg atctgctgca
    17761 gcgcattcaa gctgcagaac cgaggcgggc tgccaggatc gttgccggct tgggattgga
    17821 gaatattccc caggatctgn nnnnnnnnng ccggaaatct gagcgggggt caaaaaacgc
    17881 gtctttcgct ggcgctgctg ttgcttcaag accctgaact gttgctgctg gatgaaccca
    17941 ccaaccatct ggatatcgcc atgctggaat ggctggagga atggttgaaa aatacccgct
    18001 gtggagcgct gatcgtttct catgaccgta tgtttctcga ccgcactgtg gcgcgcattc
    18061 tggaattgga tgcatcttca cagaggctgc gttcttatga aggaaattac agcgcatatc
    18121 tcgaacagaa agaagcggaa attgaagctc agtggtctgc ctacaacgat cagaaagagg
    18181 aaattcgccg tgtgcaagcg gatatcgcac gggtgaagga gcaggcaaaa tacactgaac
    18241 ggcaagccag tgccaaccat accggcgatc cagagaagaa gttttcaaag gattttaaga
    18301 aaagattggc gaaaaaagtg gcaagaaaag ccgcagcgcg cgaaaaacgc ctggagcggt
    18361 ataaagattc cgatgagcgg gtggagaaac ccaagggacc tcgcagcatg cgggtcgaat
    18421 ttgaaaatgc tccccacctg gggcgctccg tcatccaaat ggaacggttg agcgtgggtt
    18481 ttgcaggctt tccgcccctg ctggagcaca tcaaccttca agtgcacgct tcccagcgca
    18541 tcgcttttac cggacccaac ggatgcggca agaccactct gctgcgcacg ctggcaggag
    18601 agctcgcgcc gttggcaggc agggttctgt tgggcagcag cgtgcatgtg ggatgggtgt
    18661 cgcaagatct gagtgctctg atccttgaaa aaagcgcact ggagcatgta tgggaatatt
    18721 ttcccaacca gaccgaagcg cgccgctttt tgggatatta cctgatcacg ggggatgagg
    18781 tgctcaagcc ctcttcccag ctcagttttg ggcagcgggc acgcctgatg ttggcattgc
    18841 tggtggtgcg cggctgcaac gtgctgttgc tggatgagcc catcaaccat ctggatattc
    18901 ccagccgcac ccggttcgag caggctttgc ggtcattttc cggggcgatt ctgatggtgg
    18961 tgcacgaccg ctatttcatt gaacgttttg ctcaggagat ctggcaggtg caggataaat
    19021 caatccaggt tggtagcgag gtatctgata cacttatggc aggtaccgta ctatgacctt
    19081 tgggaagaat atcctcatgc tgtccactcc tatacgctgg cttgcatcta tggaggcttg
    19141 cattccatcg ctgaatgcat caacctcacg aaggggtttg agaaaaagca gcagggtaaa
    19201 aaagttagcc gaccaccgcg ttctctaaaa ttgagaacgt gtgcatattg cagtcgattt
    19261 cggctttgac gccgtatggc aagatgaaca tggggtcagt gtgcccaaaa tccatgtgcg
    19321 tgatgatggg caaagcggtc aatccctgtt catcccgcac cacttgcaaa atggctgcat
    19381 cgtacagctc aaaatcttgc actgaggtat ctcctccagg tcgccccaag aggatgccag
    19441 aaagtttctc caggataccc atgcttgcat aggtgcgcag cccgcgttgc acggcaacag
    19501 gcggaggagc ctcttcagaa gtatccagga acagaatggc atcctgccaa tcttggagtt
    19561 ctggaaaaaa ggaagtgcct cgcaaccaat ccagcacttc aaaacaaccg cccagcaggt
    19621 gcccttgatg aatgcctgtt ccttgtagaa ctttccatcc gctgcacgca tgaagcgcgc
    19681 gtttctttgc ttgattggac ggatccgccc aatccagacg ctggcttgtc catccttctt
    19741 cattgggatt gatcacgcca attggttcac tggagaataa tgttctgcga acggaatcga
    19801 ccatgtaagg aaaaagcccg ccattttccg cgaagccagc catgatggat ggaccgtaaa
    19861 aggaggtgag ccctgcccgg taacatgcca tatggctgat ggttgtgtcg gaataaccca
    19921 tgaagatctt gggattggct cgcatgatct caaggccgag gtggggcaac aggcggatgg
    19981 aatcgtcgcc accaatggtg gaaatgatgg ctttgacgga tgggtcggaa aaagcccgca
    20041 tcagatcctc cgccctggct gcggggttct tcgccagcca atctgcatct ttcaaagtgt
    20101 tgggcatttc gaccacctgc actccgaaag ctgcctcgag ttgcttcttg ccggcttcat
    20161 aacggtgtgg aaaagtcgcc gctgttcctc gggagaggga aactgctgcc acgatgtcac
    20221 cggcttgaag tttgtgtggt ttgcgcatgg tccattgatg aattcatttt acattgccag
    20281 atcgcaaaga aacagcaggc gatcaggaat tggcttatat ggatgaaatt ttaccgtttt
    20341 ttccttcctt cgaagtattt ttatgaaacg agcgacgcaa caggcgatcg agcgaataga
    20401 agatagacag caaaaaaata agccccaatg taaaatcggt caaggaaatt tgaagtcact
    20461 gattgatatt ttcatagatt ggagaggtcg agtatctttc gtgccgccga gtcatcggtt
    20521 accaggatat tcatgtactt tccgctcaaa gctgcgtgaa tcgacttcgc tttgttttct
    20581 ccaccagcga ccgcgatcat ataaggaatt cctttttgaa ggtacaatcc gagccccagc
    20641 actcttttat taatgtcagc gtcgaga
//
LOCUS       DAOSDS010000035        20484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1114_length_20484_cov_5.025601, whole genome shotgun sequence.
ACCESSION   DAOSDS010000035 DAOSDS010000000
VERSION     DAOSDS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 20484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20484
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1114_length_20484_cov_5.025601"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /gene="tnpA"
                     /locus_tag="PLL88_05730"
     CDS             complement(<1..222)
                     /gene="tnpA"
                     /locus_tag="PLL88_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOJ01101.1"
                     /translation="MTSVDKSLAHTKWMSKYHIVFTPKYRRKMIYNKMKRDIVEIIKE
                     LCKWKGIEIIEWKAMVDHIHLLVSIPPKMS"
     gene            complement(626..1453)
                     /locus_tag="PLL88_05735"
     CDS             complement(626..1453)
                     /locus_tag="PLL88_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HOJ01102.1"
                     /translation="MKRILQRQLPDGSWRYRGNRPGQEFGENYELIETWKMLRLLVGK
                     YSFHRDHPATRQAAEYVFSCQSEEGDIRGILGNQYAPYYTGALLEVLIKAGYADDQRV
                     LRALDWLLTMQQKDGGWIIPLNMYKQSYYYEIALKAPIPPDKELPSSHMTTGMVLRGL
                     CAHPDYRNKQAVQRAGELLAERLFKEDAFTFRQAASYWFKLQYPYWWTNLLSALESLA
                     ALYFKSYDPRIRKGLEWFRENQNPGGTWTSSYEAKDPDADGWITYAVCRMVKSFLGE"
     gene            1783..2367
                     /locus_tag="PLL88_05740"
     CDS             1783..2367
                     /locus_tag="PLL88_05740"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ01103.1"
                     /translation="MEAEYIQQVLDGDEDAFTDIVEHFQSPVYNLCYRMLGTPQEAED
                     AAQESFIRAYRYIHRYDPKRPFATWLLSIAAHYCIDQQRKRHLLSVDLDEIVEFGIMD
                     DSPDPEKMVVDGEQSELIQKHLAALPVKDRMVLILRYWYDFSEDEISQALEISRSAVK
                     SRLHRARQHMAEQWTSEGSNFPEKKGRQHEPQTI"
     gene            2348..2899
                     /locus_tag="PLL88_05745"
     CDS             2348..2899
                     /locus_tag="PLL88_05745"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOJ01104.1"
                     /translation="MSHKQYKYWILDDSPLNAEEKRLLQAHLAACGDCRKLKKGWEKS
                     LHAIRATERKNPTPDFSVRWQAKLQQEKKRNRIVRQRVIIFSSLLLIGLALIAYVILS
                     GSLTAFLAHMITSSTQILLFLTKGFSELASLINGVPSIVRWALGVFFIGVANMFVILL
                     SYLVWKARKNHKEWQRAGVYAEE"
     gene            2886..3887
                     /locus_tag="PLL88_05750"
     CDS             2886..3887
                     /locus_tag="PLL88_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOJ01105.1"
                     /translation="MLKNKNFLLILLFSFLFITGCTSINTIQETRDGTLVIGEVYTLA
                     SDQVLFGDVVVIGSSFTMEEGATIHGDITLIGSTTAISGKVIGDIFILGGSTTLTSSA
                     AINGNIDQLFHEIDIEPGADILGTIDTYSNPFAFRQTWKGISTIFPYLANPERVLTSR
                     MISSIAFCLLACLITYLLPKPTQNSIRTIRNQPGTSWGIGFLALLLVPLIILILAITI
                     CLAPIALVLAVLFMIAMLFGWIALATIIGEKFNQWFYLRMAPVLQTLVGSMLISVGIS
                     LLTLIPCLGIAISMLMGCYGLGGVIISRFGKIEDQGDKRGKRSSSLPTPGDAPQIEK"
     gene            4004..6151
                     /locus_tag="PLL88_05755"
     CDS             4004..6151
                     /locus_tag="PLL88_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HOJ01106.1"
                     /translation="MKQVLQYMSTGETKIVEVPVPQVQRKTALVQTATSLVSAGTERS
                     LVEFAEKNLVQKASSRPDLVKQVTEKAKREGILTTLDSALNRLDQPLVLGYSSAGTIL
                     EIGEGLEGFQVGDRVVCGGGNHAVHAEYAVVPQNLMAHLPDEVPFEPACFGTLGAIAL
                     NGIRLAHLEIGNRVAVVGLGLLGLLTARLAEANGCLVTGIDTNSDRVQFANKAGMHSF
                     SRDKFPAITSSLTNGYGFDAVLICAATSSDDTVQLAGEIARDRGHVISLGVVGLHIPR
                     KLYYEKELHFQVSRSSGPGRYDLHYEEEGHDYPLGYVRWTEGRNLEAFVSLLHSGKIK
                     VSDLITHQFPIQDAGKAYELITGKAKEQYLGVLLTYPQTKEKISARVSFQNTIPAADT
                     VKVGVIGAGNYASNTFLPAMRKNKDTHFIGIASINGVNAQHAASRFGFSYATSSAQEI
                     IKDKTINSVVIMTRHDEHAALCSQALKANKHVYCEKPLALTIEDLAAIETLLAKPESP
                     LLMVGFNRRFAPLSVHLKDFFSHRSEPLFAHYTVNAGFLPSNHWLHDPVQGGGRIIGE
                     GCHFIDYLTFLIDQPMVAVQAVALPDQGKYHQDNMHITLEYADGSIGSIAYLANGAKT
                     YPKERVEVFCGGRIAVLDDFRALDLISSARKQSYRSHFRQDKGHRASWAAFVDAVQGG
                     KAAPIPYDQLIAVSKAAILAGKAAAEKTRLEFA"
     gene            6156..8075
                     /locus_tag="PLL88_05760"
     CDS             6156..8075
                     /locus_tag="PLL88_05760"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOJ01107.1"
                     /translation="MRWIERLQNILKSSSLLGGKTVAQYALYQIGLRTGYLKARTRQP
                     DFSILRTAISPASVKILPDLSVAELKELIGNRLPQLIQEADDVAEGKVILFDALPAQL
                     DLSPHSPLLHWSDIERGKYQLEGDIKLIWEPARLGWAFALSRAFFLSNDARYLNAFRN
                     YLDAFKQLNPPYLGSNWTSGQEAALRILALSFAHHLFSQSDAFPNDLLHEIRMVVAVH
                     AQRISPTLCYARSQRNNHLISEALGLYTAGVLLEGYKAARKWRRLGWKIFNQAIVDQI
                     APDGTYIQHSANYHRLMLDEALWMAALSDFAGEPLPDRVRQKLTASTESLFSMLDPVS
                     GCSSNLGHQDGSNVLPLSKADHLDYRPTLQAAGRLFLKNDLFKPGPWDEKACWLGIET
                     QKIQAHPPTRALRLGNEEDWASMRAVRYRSRPAHADQLHVEIWHQGQNLAMDAGTYMY
                     NAPEPWCNALRSTRVHNTLTVDGLDQMLPTSRFMWLDWANAEVSTAGTNSISARHDGY
                     RKLGVIHQRTLRSPSNSSWEIRDEVLASPAKTHAITLHWLLPDLPFNIDDHCIHFSQP
                     AFVLKFSNDRALPADMLVIRAGALIHGKSDINTTIYGWFSPTYGVKHAALSVLYTLHT
                     DLPIAVFSHWNFLKK"
     gene            8100..9311
                     /locus_tag="PLL88_05765"
     CDS             8100..9311
                     /locus_tag="PLL88_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ01108.1"
                     /translation="MHILLIHQAFATIDEAGGTRHHELARALVQEGQRVTIIASPISY
                     LTGKADREYKKIVQENGITIFRVSTYPALHRSFFHRLISFFSFMNSSFFQAMRVPEVD
                     LVWGTTPPIFQGLTAWAVARLKGVPFVFEVRDLWPAFAIAVGVLKNKTLIRLSQGLEK
                     FLYHRADALIVNSPGFIEHVRQRGATAVHLVPNSVDPAMFPPHEDGNAFRQQHGWKNK
                     FLVLYTGAHGMSNDLPVVIEAAELLKYSEDIHFVLLGDGKEKPALMDLVQQKGLQNIT
                     FLPSVEKKEIGAVMAAANCCIAILKPIDLYKTTYPNKVFDYMAAGKAIVLAIDGVIRE
                     VVEDARCGIFVRPGDAAAMAQAIRSLYGAPKKCEEMGQRGRVYVEQHFNRQSTAQQLL
                     QVLQKVVKKNG"
     gene            9304..10005
                     /locus_tag="PLL88_05770"
     CDS             9304..10005
                     /locus_tag="PLL88_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ01109.1"
                     /translation="MAEKILVVEDDLTLQETLVYNLKHQGYTVVTAADGNAAVVMAKT
                     ENPDLIMLDIMLPGMDGFEVCRIIRKDLTTPILMLTARDDEIDRVVGLEVGADDYLTK
                     PFSMRELIARVKALLRRSRIIQEHAPSAPDEKKSYVFDNLSIDQVRREVKINDQIVEL
                     KPKEYDLLLYFAENKGHALSREQILEQVWGWDYFGDSRTVDVHVRWLREKIEINPADP
                     KRIITVRSAGYRFDG"
     gene            10011..11375
                     /locus_tag="PLL88_05775"
     CDS             10011..11375
                     /locus_tag="PLL88_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ01110.1"
                     /translation="MLTLFWQLFLSFTLITVLSFLSLARGAFTTNTPLELLRSSYPYL
                     ILLFIVEAILAFAISKICQNPLKEIRRYLSNPHESEKRVRSFINRADEVGALASAVIK
                     STTQSNAMIADLAAQQRYLASILENLNDGICIVDKDGFVRILNQAAQRMFAIPQNFVS
                     GQPLIETIRHFRVQELWQNCKEKGLQQTVQLETSPERNFLQCIATPLDPAEEGNILIL
                     FQDITRLHQLEVIRRDFVSNVSHELRTPLTSLKLITETLSDGAIDDQAVQKRFLEQMV
                     KEIDNLTQLVQELLELSRIESSLVPLQKKYVKSCALIENATKRMQMQAERAGLTFTWD
                     CSPELPDILIDIDRLEQVLINLIHNAIKFTPPGGKIHVSAEQKDTAIVFSVQDSGVGI
                     PPKDRDRIFERFYKADRSRSQSGTGLGLSIARHLVEAHRGKIWVDSAVDKGSIFYFSI
                     PINQ"
     gene            11473..12378
                     /locus_tag="PLL88_05780"
     CDS             11473..12378
                     /locus_tag="PLL88_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ01111.1"
                     /translation="MSRKFFVLLSTMLVSALILGACSTAAEAAPTAEEVTAPAEETAP
                     TAEPLSGTLQIAGSTTVQPLAEVLAETFMEANPDVVIEIQGGGSSVGVTSAGDGTVAI
                     GSVSREIKSSELETYPDLAIYTIAYDGIAVIVNPELDIPSLSVEQVRGIFSGEITNFS
                     EVGGPDAEIVVVSREEGSGTRAAFEELVMVYKDENGETVETSIVENALLQQSNGQIST
                     VVSTTPNTIGYLSFGYLSDAVKGIAIDNVDPTVENVKNGTYSIYRPLSLLTKGEAQGL
                     AKAFIDFILSDAGQEIVAEDYITVQ"
     gene            complement(12360..12860)
                     /locus_tag="PLL88_05785"
     CDS             complement(12360..12860)
                     /locus_tag="PLL88_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01112.1"
                     /translation="MPGAVACRIVFNKNFPKFENISPKERTSFPAKTALEKSKIWRIR
                     IPIRITISMDSKQVRAQFCSVKNPLLRRKNPKLRIIPANKATHLFTGKVTMEKNRRIP
                     NTRKAMDNSSVNNVKREIKMGLFLPFGEVNVFRSMIALAVEQENGAQKNVCPILPMIE
                     SYCTVM"
     gene            12823..13818
                     /gene="pstC"
                     /locus_tag="PLL88_05790"
     CDS             12823..13818
                     /gene="pstC"
                     /locus_tag="PLL88_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOJ01113.1"
                     /translation="MLKTILHATAPGIIFFFLYKFDDVFSETPKQRHDFDRIIRNFLM
                     GTALSAILIVILIFFFTFQESELAITEIGLKKLLTGTIWRPGAIIGTENAQFGLVPMV
                     IGSIYSTLGAILIGLPLSLGTAILLAEIAPDFVRNIFRTSIELLSGIPSVVYGLFGMV
                     VVAPMIRQIPVEGNTTGFGILNASIILSIMILPTITNITEDAIRAVPNSYREASYALG
                     ATHWQTIKDVVLPAANSGILAAVILGISRALGETMALIMVIGNSIAIPKPINENPLSV
                     IFATARTLTGNIAVEINYAAGVHRSALFFTGVLLFVFLLLINRLANRIMRGNKTK"
     gene            13815..14747
                     /gene="pstA"
                     /locus_tag="PLL88_05795"
     CDS             13815..14747
                     /gene="pstA"
                     /locus_tag="PLL88_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOJ01114.1"
                     /translation="MNQPLTKQRELIRLKKTSREFKNKRRRFSQTIAFAIIWTLGITA
                     IALLIWIIGYVFIKGFPTINLEFLTTRPAGGFDGEGGISTTIVTTIYLVVFTIILAAP
                     LGIGAAIYFIEYANEIKNRNRFAAWLIKTARTGVEILAGVPSIIFGLFGFAVFVSYMK
                     LGFSLLSAGLSGACLVLPVIIRTTEEALLAVPESYREGSLGLGATKWQTVTGVVLPAA
                     LPGIVTGIVLSVGRIISETAIFWVTLGGSYRLPQNLMSSGRSLALHVYMLATETRAFD
                     KAMGTSAILIVIIMILNLAINVSSKYFQKRLIGK"
     gene            14753..15538
                     /gene="pstB"
                     /locus_tag="PLL88_05800"
     CDS             14753..15538
                     /gene="pstB"
                     /locus_tag="PLL88_05800"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOJ01115.1"
                     /translation="MRSTKLNGNSKIIAKSFNLFYGEFQALKNINVEIPQNAITSIIG
                     PSGCGKSTFLRSINRMNELIPGVRVDGDILLDNEDIFAQDVVELRKRVGMVFQRPNPF
                     PKSIFDNVAYGPRAHGLKNKVLLEEIVERSLTAAALWDEVKDILSRSGLALSGGQQQR
                     LCIARALAVEPQVILMDEPASALDPIATLKIEELIQNLKKEYTIVIVTHNMQQAARAS
                     DYTMVFNMNPDRSGYIIEYGKTTQVFTNPREKITEDYISGRFG"
     gene            15563..16246
                     /gene="phoU"
                     /locus_tag="PLL88_05805"
     CDS             15563..16246
                     /gene="phoU"
                     /locus_tag="PLL88_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOJ01116.1"
                     /translation="MPRETLDSKLTQIQNEIFLLGSMVEQAILDATNTLKTRDLKEAH
                     RIYEEDAIINEKRYALENAIIILLATQQPFARDLRLLAAMLLVVNELERMADYAKGIA
                     KININLGNSDIDIPVRDFQKMAEQSVSMLHRGLTAFIEQNPNLAAAIPKEDDLVDEMY
                     TKIYHNTIQYAIANPSHMDEANDILWIAHNLERFADRITNICERTIFITTGELMEMSS
                     NDDEEYLAE"
     gene            16395..17159
                     /gene="tmk"
                     /locus_tag="PLL88_05810"
     CDS             16395..17159
                     /gene="tmk"
                     /locus_tag="PLL88_05810"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOJ01117.1"
                     /translation="MLRPNPTQGKLIIVEGIDGSGKSTQIDLIYKWLEDKGKSVYFSE
                     WNSSSLVKSTTKLGKKERLFTPTTFSLIQAIDFADRWENHLFPLLKAGVIVIADRYAF
                     TAFARDVARGVDPKWVRNLYSFAIQPDLALYFQVDIETAVERILTSRAKIKYYEAGMD
                     LKLSDKRTESFKLFQGKVLEEYKKMITEFNFKVIDAKKPVENQQKEVRNLVNGMLRGW
                     EGLPNPTLSAKRYEKKQREAETVQGGADYGKNEILR"
     gene            17134..17844
                     /locus_tag="PLL88_05815"
     CDS             17134..17844
                     /locus_tag="PLL88_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HOJ01118.1"
                     /translation="MAKMKFYGNGFPYMPKEHIKGKLIVLEGTDGVGRSTQVTMLKKW
                     LEKNNYAVSDTGLRRSPLTQPGLDRAKEGNTLTPTTLSLFYATDFADRLENQIIPALR
                     AGFYVLSDRYFYSIIARDVVRGLNPQWAREVYGFALVPDLVIYLKADISTLVTRIVHG
                     RGFNFWESGMDIIKEQDLYDSFVQYQKSMIEQFDQMAEEYKFRVIDANRSVKAIFEDV
                     KSLVEELIIPNSESKTKK"
     gene            17857..18882
                     /locus_tag="PLL88_05820"
     CDS             17857..18882
                     /locus_tag="PLL88_05820"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOJ01119.1"
                     /translation="MKVNRDIRIQKYASANILGNTQNLLAILEKIQNRYKIEDIHDLR
                     VASRRIRTALSIFAPFFPKSKIQGWGSTIRSITSDFGKTRDLDVQIEFLENQLNAISD
                     RKVRAGINRLQLRLIQNRKKRDRKVKKHTTSLLKSKDLNSLLNTMQRISQTPGEENYP
                     VELYQLAFHTIYNALDQFLYYEVFIQHPENIHELHLMRIAAKKVRYTLEVFLPLYQGK
                     MDAFLDIMKNIQQKLGAIHDCDVWITFIPEFMEAEKQRTLKYYGRSSAFHRFIPGLEF
                     IKKDRQQERDRIYTEFMNEWQKWRSAETWLRMRELILQASVSVMDNTPINTDGSRGNE
                     KNANISG"
     gene            18863..19669
                     /locus_tag="PLL88_05825"
     CDS             18863..19669
                     /locus_tag="PLL88_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOJ01120.1"
                     /translation="MRTFQDRKISNPIQKIALVSDIHANLPALEAVYRHAIQHDVQLF
                     LNAGDTVGFGPFPNECLDFIRLKKMVSVKGDYDQKVLEYPKRKNSLEKKKNPLQLFSF
                     KWAYEQLTEHNFRFLADLPEFQCIPIGMKKVYIAHGSPMSIKAHLGSTTTHSELVANQ
                     KIAQSDFIICGNSHVYWSSQVKDTLFINPGSVGRQDDGDPRASYATLSLSDPIKVDHY
                     RVPYDISRVLQAIEVYNLPVEFSYMFQRGVNLERAIAELSNAKNDQISTA"
     gene            complement(19775..>20484)
                     /locus_tag="PLL88_05830"
     CDS             complement(19775..>20484)
                     /locus_tag="PLL88_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HOJ01121.1"
                     /translation="DAELLMQYDSMADLRVAEMRNALQILGVHEYYFLGYRDSGMPGS
                     PDNNHPQAFIQAPLEQTGMDIAYYIRKIRPAVVLTSDPMGGYGHPDHIHAYRSTERAL
                     ELAADERIHIEGMSPFHPAGVYLHTFPRKFMRLVVKLMPLVGKDPTKFGKNGDINFVE
                     VMEADFPINVRIHYGKYAVLREKASACYRSQGGDRQSGFFIAWLERLISSSEVYMQYF
                     PQPSARRIKKDLFDLET"
BASE COUNT         5614 a         4921 c         4656 g         5293 t
ORIGIN      
        1 gctcatcttt ggcggtatgc tcaccagcag gtggatgtga tctaccatcg ctttccactc
       61 tatgatctct atccctttcc acttgcataa ctccttgatt atctctacta tatccctctt
      121 catcttgtta tagatcatct tccgcctgta cttcggtgtg aatacaatgt gatacttact
      181 catccatttc gtgtgtgcta aacttttatc cacggacgtc actccttctt attttgatgg
      241 cttgaacttc catcatttta tcgggagtga ctcccttttt ctataagtcc tttccctcca
      301 cccgcttagc gggtggtttt tgtctcggac gtcccgtcct caactcgcta aagcgttaat
      361 aagattaaca agcaaattta aaagtagtcc tgttctatac tatccattcc aggtttgtat
      421 cttaagtatt ggcatcataa ttaaaactga tattgataaa gttttaacga taatttccta
      481 agttaaaaat taaatcagaa cactacaatt tatcatagcg ataaggcaag gtagccttaa
      541 tgccattgat tagttaataa ttagtgaacc tgctttattg aacacactct ttaaagcagg
      601 ctcatatata tacgctgatg agagatcatt ctcccagaaa gctttttacc atacggcata
      661 ccgcgtaggt gatccaacca tccgcatccg gatccttcgc ctcatagctg gaagtccagg
      721 ttccaccagg gttttggttt tcacgaaacc actcaagccc tttgcggatg cgcgggtcat
      781 atgatttaaa gtacaaagca gccagcgatt ccaaagccga gagcaggttt gtccaccagt
      841 agggatactg cagtttgaac cagtagctcg cggcttgtct gaaagtaaag gcatcttctt
      901 tgaaaagtcg ctctgccaat aattcaccag cgcgctgtac tgcctgttta ttgcggtaat
      961 ccggatgagc acacaaccct cgcaaaacca tgccggtggt catgtgagaa gacggtagtt
     1021 ccttatcggg tggaatgggt gctttgagag ctatttcata ataatatgat tgtttgtaca
     1081 tgttgagcgg gatgatccac ccaccatcct tttgctgcat ggtaagcagc caatccagag
     1141 cgcgcaacac ccgttgatcg tcagcataac cggctttgat gagaacttcc agcaacgcgc
     1201 ctgtataata tggcgcatat tgatttccga gaatgccacg gatatcccct tcttcgctct
     1261 ggcatgaaaa gacatactct gctgcctgcc gtgtggctgg atgatcgcga tgaaatgaat
     1321 attttccaac cagcaaacgc aacattttcc aggtttctat cagttcataa ttctccccga
     1381 attcttgacc cgggcgattg ccacgatatc gccaactgcc atcaggtagc tgacgctgta
     1441 aaatacgttt cacgggtcta ctgctccaaa cttctgaaac ctgctgccct ttctgatgga
     1501 gtaaatccct tgcaatcaaa aaatgcaatc caggatcgtt gaccgataaa agggcaggca
     1561 tcggattatg aacaaaataa tccttccaca tggttatgct ctcaatcctt tgaaataatc
     1621 tataatggat aataatggaa gattgggcaa ttttcaatcg tctgataata cccgttcatt
     1681 ggaatattat gcggtcttca gaacggttga aacctttttt atctagaatc gtatatgggt
     1741 ataaataaac aatctttgga gccacgcttg gaaaccagct tcatggaagc agaatacata
     1801 caacaggtgc tcgatggaga cgaggatgcc tttacggaca tcgtcgaaca tttccaatcg
     1861 cctgtttata atctctgcta tcgcatgctg ggcacaccac aagaagcaga ggatgccgca
     1921 caagaatcat tcatcagagc ataccgctat atccatcgct atgaccccaa gcgtcccttc
     1981 gcgacatggt tgctatccat tgcagcccat tattgtatcg atcaacaacg aaaaaggcat
     2041 ttgctctcgg ttgatctgga tgagattgta gaatttggta ttatggacga ttcaccggac
     2101 cctgaaaaga tggttgttga tggggaacag agcgaactca tccagaaaca cctggcagct
     2161 ttgccggtaa aggatagaat ggtattgatt ttgagatact ggtatgattt ttcggaagat
     2221 gagatcagtc aggcattaga aatttccagg agtgccgtaa aaagcagatt acatcgcgcc
     2281 agacaacaca tggcagaaca atggacttca gaaggatcaa attttcccga gaaaaaaggg
     2341 aggcagcatg agccacaaac aatataaata ttggatccta gatgattcac ctttaaacgc
     2401 agaagaaaaa aggctgctgc aagctcacct ggcagcctgc ggtgactgcc gaaaattaaa
     2461 aaagggttgg gaaaagagtc tgcatgccat ccgtgccacc gaacggaaga accccacccc
     2521 ggatttttct gtgcgctggc aggcgaaatt acagcaggag aaaaaacgaa acaggatcgt
     2581 aaggcagcga gtgatcatat tcagcagcct ccttttaata gggcttgccc tgatcgctta
     2641 cgtgatctta tcaggctcct tgacggcttt tttggcacac atgatcacct ccagcacgca
     2701 gatcctcctg ttccttacca aaggattttc tgaactggcg tcactcatta acggtgtacc
     2761 ttccattgtt cggtgggcat tgggagtctt tttcatcggt gttgccaaca tgttcgtcat
     2821 tttgctttct tatctggttt ggaaagccag aaagaaccac aaggaatggc aaagagcggg
     2881 ggtttatgct gaagaataaa aacttcctgc ttatccttct cttctctttc cttttcatta
     2941 cagggtgcac ttcaataaac accattcaag aaacacgtga tggcacattg gtcatcgggg
     3001 aagtatatac ccttgccagt gatcaggtct tatttggtga cgtggtggtc attggatcaa
     3061 gtttcacaat ggaagaaggt gctaccatcc acggcgatat caccctcatc gggagcacaa
     3121 cagcaatctc aggaaaagtc ataggggata tcttcatcct cggcggatca accaccctta
     3181 catcgagcgc agctatcaat ggcaatatcg accaattatt ccatgaaatc gatattgaac
     3241 ctggcgcaga tattttagga acgatcgata catattccaa tccattcgct ttccgccaga
     3301 cgtggaaagg gattagcacc atcttccctt atctagccaa tccggaacgg gtgctgacaa
     3361 gccgcatgat aagttccatt gctttctgcc tgttagcatg cctgatcact tacttattgc
     3421 caaaaccaac acaaaacagc attcgcacta tacgcaatca acccggcaca agctggggca
     3481 tcgggtttct ggcattgctg cttgtacccc tcattattct gatcctggca atcacgatct
     3541 gtctcgcccc tatcgcactg gtgcttgcag tgctgttcat gatagcaatg ctctttggat
     3601 ggatcgcgct ggctaccatc attggtgaga aattcaatca atggttttat ttaagaatgg
     3661 cgcctgttct ccaaacattg gttggatcca tgctaatctc agtcgggatc agtttactca
     3721 cccttattcc ctgcctgggt atcgccatca gcatgctcat ggggtgttat ggattgggcg
     3781 gtgtgatcat cagtcgcttt ggcaaaatag aggatcaagg cgataagaga ggcaaacgct
     3841 cttcaagtct tccaactcca ggcgatgcac ctcaaattga aaagtagatc aataatctct
     3901 tgcgttcttc atcaatcaaa gaagctgggc gtcgatccag cttctttttg atattcggga
     3961 ttaaatagtt ataatttcat cattcaacat acacgaggta ggaatgaaac aggttctgca
     4021 gtacatgagc acaggagaaa ccaagattgt agaagtccct gttccgcaag tgcagagaaa
     4081 aacagcgctg gtgcaaaccg caacatcatt ggtttccgca ggcaccgaac gttccctggt
     4141 ggaatttgct gaaaagaatc tggtccaaaa ggcttcatcc cgtccagacc tggtaaaaca
     4201 ggtcacggag aaagccaaac gtgaaggcat cctcaccacg ctcgattccg ccctcaaccg
     4261 gcttgatcaa ccattggtgc ttgggtactc ttcggctggt actattcttg aaattggaga
     4321 agggttggaa ggcttccagg ttggtgaccg cgttgtgtgc ggtggtggga atcatgccgt
     4381 gcatgccgaa tacgcagttg tcccgcagaa cctcatggct cacctgcctg atgaggttcc
     4441 ttttgaacct gcctgttttg gtactttggg tgccattgcc cttaacggaa tccgccttgc
     4501 ccatttggaa atcggaaacc gtgtggcagt ggtaggtctt ggtttattgg gtttattgac
     4561 cgcccgcctg gcagaagcaa acggctgtct ggtaaccggc attgatacaa actctgatcg
     4621 ggtccaattt gcaaataaag cggggatgca ttccttcagc cgcgataagt tccccgccat
     4681 aacctcctct ctgacaaacg ggtatggttt tgacgccgtg ctcatctgtg ctgccaccag
     4741 cagtgatgat accgttcaat tggctggcga gatcgcccgc gaccgcggtc atgtcatttc
     4801 attgggtgtg gtcggattgc atatcccccg caaactttat tatgaaaaag aactccactt
     4861 tcaggtttcc cgctcctctg gtccaggtcg ctatgatctg cactatgaag aagagggtca
     4921 cgattacccg ctggggtatg tgcgctggac ggaaggtcgc aacctggaag catttgtcag
     4981 tttgctgcat agcgggaaaa taaaggtaag cgatctcatc acccatcaat ttccaattca
     5041 ggatgccgga aaggcatatg agctcattac cggtaaagcg aaagaacagt acctgggtgt
     5101 gcttctcacc tatccgcaaa ccaaggagaa aatttctgca cgggtctctt tccaaaacac
     5161 cattccagcg gcagatacag taaaagtggg ggttattggc gcagggaact atgccagcaa
     5221 cacttttctt ccggctatgc gcaagaacaa ggacacccac ttcatcggca tcgcttccat
     5281 caacggcgtc aacgcacagc atgcagcttc caggttcggt ttttcttatg ccacgtcttc
     5341 cgcacaggag atcatcaaag acaagaccat taacagcgtt gtgatcatga cccgtcatga
     5401 cgaacatgca gcgttatgct cgcaagcctt aaaagcgaat aaacatgttt actgcgaaaa
     5461 gccgctggcg ctcacgatag aggatctggc agccattgaa acactattag ccaagccaga
     5521 atcgcccctg ttgatggtgg gtttcaatcg tcgctttgcc cctctgtctg tccatttgaa
     5581 ggatttcttt tctcaccgtt ccgaaccgct tttcgcgcat tacactgtaa atgccggctt
     5641 tcttccttcg aatcattggt tgcacgatcc tgtgcagggc ggcgggagga tcattggaga
     5701 gggctgtcac tttattgatt acctcacctt tttaatagat cagccaatgg tcgccgtaca
     5761 ggcagttgct ctgccggatc aagggaaata tcaccaggat aatatgcaca tcaccctgga
     5821 atatgctgat ggttcaatcg gcagcatcgc ctatctggct aatggcgcca aaacatatcc
     5881 aaaagaacgg gtggaggtct tttgcggtgg aagaatcgcc gtattggacg acttccgcgc
     5941 cctggatttg atttccagcg cacggaaaca aagctatcgc tcgcacttcc ggcaagataa
     6001 agggcaccgg gcgtcttggg ctgctttcgt cgacgccgtc cagggaggaa aggcagcacc
     6061 catcccgtac gaccagctca tcgcggtcag caaagctgcc atcctggcag gtaaagctgc
     6121 cgccgaaaag actcgattgg aattcgcgta aaagaatgcg ttggatcgaa cggctgcaaa
     6181 atatcttaaa gagctcctcc ctgcttgggg gaaaaacggt agcgcagtat gctctctatc
     6241 aaattggact gcgcacgggg tacttgaaag cccgcaccag gcaacctgat ttttccatct
     6301 tgcgcaccgc tatcagcccc gccagcgtaa agattttgcc ggatctttct gttgccgaat
     6361 tgaaagagct gatcggcaat cgactcccgc aactgatcca agaagctgac gatgtggcag
     6421 aggggaaggt tattttattc gatgcattgc cagcccaatt agacctgtcg cctcatagcc
     6481 ccttactgca ttggagtgac atcgaacgag gaaaatatca gcttgaaggc gatatcaaac
     6541 tcatctggga acccgcccgt ttgggctggg cattcgcctt atccagggcg tttttcttga
     6601 gcaacgatgc ccgctacctg aacgctttca ggaattacct ggacgcgttc aaacagctca
     6661 acccaccata cctgggaagc aattggactt caggtcaaga agccgcttta cgcatcctgg
     6721 cattgagctt tgctcatcac ctcttttcac aatccgatgc ttttcccaat gatctcctgc
     6781 acgaaatacg aatggttgtg gctgtacatg cccagcgcat ctctccaacg ttgtgctacg
     6841 cgcgatccca acgcaacaat cacctcatta gcgaagcgtt ggggttatat accgctggcg
     6901 tattgctaga agggtataaa gccgcaagaa agtggagacg gttgggttgg aaaattttta
     6961 accaagccat tgtagaccaa attgcccccg atggaaccta tatccaacac agtgcgaatt
     7021 accatcgttt gatgcttgat gaagcacttt ggatggcagc gctttcagat tttgccgggg
     7081 aaccattgcc cgatagggtc aggcaaaaac tgactgcttc caccgaatct ctattctcta
     7141 tgcttgaccc cgtctcagga tgttcctcca acctggggca ccaggatggt tcgaacgtgc
     7201 ttccattgag caaagctgat catctggatt atcgccccac cctgcaggca gccggaagat
     7261 tgttcttgaa aaacgatcta tttaaacctg gtccgtggga cgaaaaagcg tgctggctgg
     7321 ggattgaaac acaaaaaata caggctcatc caccaacccg tgcattgcgg ttgggaaacg
     7381 aggaggattg ggctagcatg cgggcagtgc gttatcgttc ccgccccgct catgctgatc
     7441 aattgcatgt agaaatctgg caccagggtc aaaacctggc gatggacgcg ggaacctata
     7501 tgtacaacgc ccccgaaccg tggtgtaatg ccttgcgttc tacgcgggtt cacaacacac
     7561 taacagtgga tggattggat cagatgctgc caaccagccg tttcatgtgg ttggattggg
     7621 cgaacgctga agtatcgacc gccggcacga attccatttc agcccggcat gatggttatc
     7681 gcaagcttgg tgtgattcac caacgcacgc tgcgatcacc ttccaattcg agttgggaaa
     7741 tcagggatga agtgttggct tcacctgcaa agacacacgc gatcacactg cattggttgc
     7801 tgcctgacct tcccttcaat attgatgacc attgcattca tttttcccaa cccgcgttcg
     7861 ttctgaaatt cagcaatgac cgggcgcttc ctgcagatat gctggtcatc cgtgctggtg
     7921 ccttaataca cggaaaatca gacataaaca cgaccattta tggatggttt tctcccacct
     7981 acggggtaaa acatgcagcc ctgtctgtac tgtacaccct ccacaccgat ttacccattg
     8041 cagtgttttc tcattggaac tttctaaaga aataaatcag aacagtgtac aatcggaata
     8101 tgcacattct tctcatccac caggcatttg caaccatcga cgaagctggc ggcacccgcc
     8161 accatgaatt agcgcgtgcc ctggtgcagg agggtcagcg ggttacaatc atcgccagcc
     8221 ccatcagcta tctgacaggc aaagcggatc gtgaatataa aaaaatagta caggaaaatg
     8281 ggataacgat tttccgcgtt tccacctatc ctgctctaca tcgcagcttt ttccaccggt
     8341 tgataagctt tttctccttt atgaattctt ctttttttca agccatgcgc gtgcccgagg
     8401 tggacttggt ttggggcacc acgcccccaa ttttccaggg attgaccgcc tgggcggtag
     8461 cgcgattaaa aggcgtgccc ttcgtattcg aagtgcgcga cctgtggcca gcttttgcga
     8521 tcgcggtggg agttctcaag aataagaccc tcatccgctt atcacaaggg ctcgagaagt
     8581 tcctctacca tcgtgccgat gcactcattg tgaattcccc cggattcatt gaacatgttc
     8641 gccagcgcgg cgcaacagcg gtccaccttg tgcccaatag tgtagatcca gccatgtttc
     8701 ctccacatga agatggaaat gcttttcgcc aacagcatgg ttggaaaaac aagttcctgg
     8761 ttctttatac aggtgcacac ggcatgtcga atgatcttcc ggtggttatt gaagctgcgg
     8821 aactgttaaa gtactctgaa gatattcatt tcgtgttact aggtgacggc aaagaaaaac
     8881 ccgcccttat ggacctggtt caacagaaag ggctgcaaaa tatcaccttt cttccctctg
     8941 tcgagaaaaa agaaatcgga gctgtcatgg cagccgcgaa ttgttgtatt gccatcttga
     9001 aaccgataga cttgtataaa acaacctatc ccaataaagt gtttgattac atggcagccg
     9061 gcaaggcgat cgtgttggct atcgatgggg tgattcgtga ggtggtggag gatgccaggt
     9121 gcggtatctt cgtccgacct ggcgatgcgg cggcgatggc acaagccatc cgttcacttt
     9181 acggagcacc aaaaaagtgt gaagaaatgg gacaacgtgg tagagtatat gtagagcaac
     9241 acttcaaccg gcaaagtact gcccaacaat tgctgcaagt actgcaaaag gtggtaaaga
     9301 agaatggctg aaaagatatt ggttgtggaa gatgacctta ccctacagga gacgctggtt
     9361 tataacctga agcatcaggg atatacggtg gttacggctg ctgacggcaa cgcggcggtc
     9421 gtaatggcta aaaccgagaa tccggatctg atcatgctgg atattatgct gcccggaatg
     9481 gatggcttcg aagtgtgccg cattatccgt aaagatctga ccactcccat cctcatgtta
     9541 acggcacgag atgacgaaat cgaccgggtg gttggtctgg aggtcggggc agatgattat
     9601 cttaccaagc ccttcagtat gcgtgaattg atcgctcgcg tgaaagcact tttgcgccgt
     9661 tcaagaatca tacaagagca tgcgccaagc gcgccagatg aaaagaaaag ttacgtcttc
     9721 gacaaccttt ccattgatca ggtgcggcgc gaggtaaaga tcaatgatca gatcgtggag
     9781 ctgaaaccca aggagtatga tctgttactt tattttgcgg aaaacaaagg gcatgctttg
     9841 agccgtgaac aaatcctgga acaagtttgg ggctgggatt attttggcga cagccgcact
     9901 gtagatgtgc acgtacgctg gttgcgtgaa aagattgaga tcaatcctgc ggatccaaaa
     9961 cggatcatca ccgtacgcag tgctggctac cgctttgatg gataacaggc atgttaacct
    10021 tattttggca gctctttctc agcttcacat tgataaccgt tctctcattt ttatcgcttg
    10081 ccaggggtgc tttcaccaca aacaccccac ttgagctttt gcgctcatcc tatccctatc
    10141 tgatcctgtt gttcattgtt gaagccattc tggcatttgc catatccaaa atttgtcaaa
    10201 atccgttaaa agaaattcgg cgctacctgt ccaatcctca cgaatctgaa aagagggtcc
    10261 gtagttttat caatcgtgct gacgaggtag gcgctctcgc cagtgcagtc ataaaatcta
    10321 ctacacaatc caacgccatg atcgctgatt tagctgcgca acaacggtac ctggcgagca
    10381 ttctagagaa tctcaacgat ggaatctgca ttgtagataa agatggcttt gtacgaattc
    10441 tgaatcaagc tgcccaacgc atgttcgcta ttccacagaa ttttgtaagc ggtcagccac
    10501 ttatcgaaac catccggcac ttccgcgttc aagaattgtg gcaaaattgc aaggaaaagg
    10561 gattgcagca aactgtgcag cttgaaacca gccctgaacg gaatttcttg cagtgcatcg
    10621 ccaccccgct ggatcccgcc gaagaaggga atatactcat cctctttcag gatatcacca
    10681 ggttgcacca actggaagtg atacggcgcg attttgtcag taatgtttcc catgaattac
    10741 gcactccact tacctctcta aagctcatca ctgaaaccct aagtgacgga gcaatagatg
    10801 atcaggctgt ccaaaaaaga tttttggaac aaatggtaaa ggaaatcgac aacctgaccc
    10861 aattggtaca ggaactgttg gaattatcgc gcattgagtc cagtttggtg cccttgcaaa
    10921 agaagtatgt aaaaagttgt gcactcatag agaacgccac gaaacggatg caaatgcaag
    10981 cggaaagggc cggtttaacc tttacctggg attgttcccc agagctgccc gatattctta
    11041 ttgatatcga ccgccttgaa caggtgttga tcaaccttat ccataatgca atcaaattca
    11101 cgccgcctgg cggaaaaatc cacgtttctg cagaacaaaa ggacaccgct atcgtctttt
    11161 cagtgcaaga tagcggtgtg gggattccac ccaaagatcg tgaccgaatt tttgaacggt
    11221 tttataaagc cgaccgttcc cgatcgcaaa gcggaacagg gttgggacta tccattgcgc
    11281 gccatctggt tgaagcgcac cgggggaaaa tatgggtcga cagtgcagtc gacaagggta
    11341 gtattttcta tttttccatt ccaatcaatc agtaaatctt agtaaattta atcaaacctt
    11401 aacctttttc tcatttcctg ttaaaagtcc ttcagtatca ttcaggcata tttcaaaaag
    11461 gagaaaagaa aaatgtcgcg taagtttttt gttttgttaa gcaccatgtt ggtaagtgcc
    11521 cttatactcg gcgcatgcag cactgctgcc gaagcagctc ccaccgctga agaggtcact
    11581 gctccagccg aagaaacagc ccccactgca gagccattgt cagggacgct gcagatagcc
    11641 ggctccacaa ccgtccaacc tctggcagaa gttttggcag aaacatttat ggaagccaat
    11701 ccagacgtgg tcattgagat tcagggcggt ggttccagcg ttggtgtcac atcagccggt
    11761 gatggaaccg tagcgatcgg ctctgtttcc cgagagatca agagcagcga actggaaacc
    11821 tatcctgatc tggctatcta caccattgcc tatgatggaa tcgctgttat cgtgaatccc
    11881 gaacttgaca tccccagcct atctgtggaa caggtgcgcg gaatcttttc tggtgagatc
    11941 accaacttct ccgaagtagg tggtccagat gcagagatcg tcgttgtttc ccgtgaagaa
    12001 ggttctggta cgcgtgcagc atttgaagag cttgtaatgg tttacaaaga tgaaaacggc
    12061 gaaaccgtcg aaacttccat tgtagaaaac gctctcttgc aacagtcaaa cgggcagatc
    12121 tcgaccgtgg tatcaaccac tcccaacact atcggttatc tctcattcgg gtatctcagt
    12181 gatgcggtca aaggcattgc aattgataat gttgacccaa ccgtagagaa tgtgaagaat
    12241 ggaacataca gcatctatcg tccattgagc cttctcacca aaggtgaagc acaaggtctg
    12301 gctaaggcat tcatcgattt catcctctcg gatgccgggc aggaaattgt tgctgaagat
    12361 tacatcacag tacaataact ttcgatcatt ggcaaaatgg ggcacacatt cttttgtgcc
    12421 ccattttcct gttctactgc tagggcgatc atggatctaa aaacattcac ttctccaaat
    12481 ggtaaaaaaa gacccatttt tatctctctt ttgacattat tcacgctact attatccatt
    12541 gctttcctcg tattcggaat ccttctattt ttttccatag tcactttccc cgtgaacaaa
    12601 tgggtagctt tgttcgctgg aatgatcctg agtttcgggt ttttccttct tagcaaaggg
    12661 ttcttcacac tgcaaaactg ggcacgcact tgcttgctat ccatactgat agtgatcctg
    12721 atcggaattc ttattctcca aatcttcgat ttttccagcg cggtttttgc aggaaaagaa
    12781 gttctttcct taggagaaat attctcaaac ttcgggaaat tcttgttaaa aacaatcctg
    12841 cacgcaaccg ctccgggcat tatctttttt ttcctataca agttcgacga cgtattttcc
    12901 gaaacaccca aacagcgcca tgacttcgac cggattataa gaaatttcct aatgggaacc
    12961 gctcttagtg ccattctgat cgtcattctg attttcttct ttacattcca agaatcggaa
    13021 ttagccataa cagaaatcgg gttaaaaaaa ttattgaccg gcactatctg gcgtcccggt
    13081 gccatcatcg gaaccgaaaa tgcacagttc ggattggtcc ctatggtgat tggatcaata
    13141 tacagcaccc tgggggcaat tctgatcggt ctcccacttt cgcttggcac agccattctt
    13201 ttggcagaaa ttgcgcccga ttttgtacgc aacatattcc gtacctcaat cgaactacta
    13261 tccggcattc cttcagtggt gtacgggctg tttgggatgg tcgtcgtggc acccatgata
    13321 cggcagatac ccgtcgaagg taatacgacc ggttttggca ttctcaatgc ctccatcatt
    13381 ctttcgatca tgatccttcc aaccataacc aatattaccg aagatgctat tcgtgctgtc
    13441 cccaacagct atcgcgaagc atcgtatgcg ttaggagcca cccattggca aactatcaaa
    13501 gatgtggtgc ttcctgcagc caattcaggc atcttagctg ctgtgatctt gggaatcagc
    13561 agagcccttg gagagacaat ggcactgatt atggtgatcg gtaactccat cgcgatccct
    13621 aaacccatta atgagaatcc gctttctgtc atttttgcta ccgcgcgaac cctaaccggg
    13681 aacatcgctg tagagatcaa ttatgccgcc ggagtacatc gatcagccct atttttcaca
    13741 ggagtgctct tattcgtttt tctattgctc atcaaccgtc tggcgaacag gatcatgaga
    13801 gggaataaaa caaaatgaac cagccattaa ccaagcagag agaattaata cgcctcaaga
    13861 aaaccagccg tgagtttaaa aacaaacgac gaagattctc tcaaacgatc gcgtttgcga
    13921 ttatctggac attgggaatt acagcaatag ccctgctgat atggatcatt ggatatgttt
    13981 ttatcaaggg gtttcctacg atcaacctcg aatttctgac cacacgccca gccgggggat
    14041 ttgacggaga aggtggtatt tccacaacca tcgtgaccac tatctacctg gttgtgttca
    14101 ccattatcct tgccgctcca ttgggaatcg gtgctgctat ttattttata gaatatgcga
    14161 atgaaataaa aaatcgcaat cgattcgcag cctggttgat caaaaccgcc cgcaccgggg
    14221 tggaaatatt ggctggcgtt ccttcaataa tcttcgggtt atttggattt gcagttttcg
    14281 tatcgtacat gaaattgggg ttttcattgt tatccgctgg cttgagcggt gcctgcctgg
    14341 ttttgccagt gattatcaga actaccgaag aagcgctgct ggctgtgcca gaaagttacc
    14401 gcgaaggttc attgggtctg ggagctacca aatggcaaac ggtaaccgga gtggttttac
    14461 ccgccgcatt accaggaatt gtcaccggaa ttgttctaag tgttggtaga atcatatcgg
    14521 aaacggcaat cttttgggtt acacttggcg gcagctatcg ccttccgcaa aacctcatgt
    14581 cttcaggaag gtcactggcg ctgcacgtgt acatgttagc caccgaaacg agagcctttg
    14641 acaaagccat gggcacctcg gctatcttga ttgtgattat tatgatactg aacctggcaa
    14701 taaacgtttc atcaaaatat tttcaaaaac ggttgattgg aaagtaggca acatgcgatc
    14761 aacaaaacta aatggaaatt caaaaatcat cgcaaagagc ttcaatctct tttatggcga
    14821 attccaggcg ttaaagaaca tcaatgtcga aattcctcag aatgcaatca cttccatcat
    14881 tggtccctct gggtgtggca aaagtacttt cctgcgttcc attaatcgta tgaacgagtt
    14941 gatccccggc gtgagggtgg atggggatat acttctggac aacgaggaca ttttcgccca
    15001 agatgtagtt gaactgcgca aaagggtcgg tatggtgttc caacgtccca atccatttcc
    15061 gaaatccatt ttcgataacg tagcatatgg tccacgtgcg cacggattaa agaataaagt
    15121 gctcctggaa gaaatcgttg aaaggagcct cacggcagca gcgctatggg atgaagtgaa
    15181 agatattctt agcagatcag ggctggcact ctcaggcggt caacagcaac gcctgtgcat
    15241 agcacgcgct ctggcggtag aacctcaggt cattttgatg gatgaaccag cttctgcact
    15301 cgacccgatc gcaacgctca agatcgaaga attaatccaa aatctgaaaa aagaatatac
    15361 aattgtcata gttacacaca acatgcaaca ggcagctcgt gcttctgatt acaccatggt
    15421 attcaacatg aaccccgacc gctcgggtta tattattgaa tatggaaaaa cgacccaggt
    15481 tttcaccaac ccacgagaaa aaatcactga agactacatt tctggtcggt ttggatagag
    15541 agagataata tagaggtgga atatgccgcg agaaactctg gattcaaaat tgacgcaaat
    15601 tcaaaacgag atattccttt taggtagcat ggtcgaacaa gccattctgg atgcgaccaa
    15661 tactttgaaa accagggatc tgaaagaagc gcatcgaatt tacgaggaag atgcaattat
    15721 caacgaaaaa cggtacgcat tggagaatgc cattatcatt ctgctggcta cccagcaacc
    15781 atttgcccgt gatctgcggc tgctggctgc catgttactg gtggtcaatg agttagaaag
    15841 gatggcagat tatgcaaagg gaattgccaa gatcaacatc aaccttggca attcagatat
    15901 cgatattcca gttcgcgatt ttcaaaaaat ggcagaacaa agcgtcagta tgctgcatcg
    15961 cgggctgacc gccttcattg aacaaaatcc caatctggca gccgccatcc caaaggaaga
    16021 tgatctcgtg gatgagatgt acaccaagat ctatcacaac accattcagt acgccattgc
    16081 aaacccctcc catatggatg aagccaacga catcctgtgg atcgcccaca acctggaacg
    16141 ctttgccgac cgcatcacca atatttgtga gcgcacgatc ttcatcacca cgggagaatt
    16201 gatggaaatg agttcaaatg acgatgagga atatcttgcc gaataattaa ttcgttttct
    16261 gagcttccta ccaagtctcg aaaagtcata ccgttcgact ccagaagtac ctttttaagt
    16321 ttgccatagc aattctggaa ataaaaatat acaataggta taaattaagg tgttgatatg
    16381 gaaatggaca tggtatgtta aggcccaatc cgacccaggg gaagttgatc attgtggaag
    16441 gcattgatgg aagcggaaaa tccactcaaa tcgatttgat ctataaatgg ctagaagaca
    16501 aggggaaatc agtgtatttc agtgaatgga actcttcttc cctcgttaaa agcacaacca
    16561 aacttggcaa aaaagaacgc ttattcactc ctacgacctt cagtttgatt caggcgattg
    16621 attttgcaga tcgttgggaa aaccatttgt tccctctatt aaaagccggc gtgattgtca
    16681 ttgccgaccg ttatgccttt acggcatttg cgagggacgt ggcacgtgga gtggatccta
    16741 aatgggtacg taatctctac tcttttgcaa tccaacctga ccttgcatta tattttcagg
    16801 tagatatcga gaccgctgtc gaacgcatcc ttacatcaag ggcaaagatc aaatattatg
    16861 aagcaggtat ggacctgaaa ctctcagaca aaagaaccga aagtttcaag cttttccagg
    16921 ggaaagtgct cgaggaatac aaaaaaatga tcacggagtt caatttcaag gtcattgatg
    16981 caaaaaaacc ggtggagaac caacaaaaag aggtccgcaa tttggtaaat ggcatgttgc
    17041 gaggttggga aggtcttccc aaccccactt tgtctgcaaa aagatatgaa aaaaagcagc
    17101 gtgaagctga aaccgtacaa gggggagccg attatggcaa aaatgaaatt ttacggtaat
    17161 ggcttcccct atatgccaaa agaacacata aaaggaaaac tgatcgttct ggaaggaacc
    17221 gatggtgtgg gcaggtctac tcaggtaacc atgctaaaaa aatggctcga gaagaacaac
    17281 tatgcagtgt cagataccgg attacgccgt tcccccctca cccaacccgg tctggatagg
    17341 gcaaaagaag gcaacaccct cacgccaacc acgctcagct tgttctatgc cactgatttt
    17401 gccgaccgcc ttgaaaatca gatcattcca gctttacgcg caggttttta cgtcctttcc
    17461 gaccgatatt tctattccat tatcgcgcgc gatgtcgtgc gtgggttgaa tccacaatgg
    17521 gcgcgagaag tttacggatt tgccctggtg cccgatctcg tgatttatct caaagcagac
    17581 atttctacac tggtaacacg catcgtacac ggtcgcggct tcaatttttg ggaatctggg
    17641 atggacatca ttaaagagca agatctttat gatagtttcg ttcaatacca gaaaagtatg
    17701 attgagcaat tcgaccagat ggctgaagaa tataaattca gggtgatcga tgccaaccgc
    17761 tcggttaaag cgatttttga agacgtgaaa tctttggttg aagagctgat cattccaaac
    17821 agcgaatcca aaactaaaaa gtaaagggta tttcttatga aggtgaacag ggatatacgc
    17881 atacagaaat atgcatctgc caatatcctg ggaaacacac aaaacctgct agcaatcctt
    17941 gaaaaaatac aaaaccgata caaaattgaa gatatccatg atttgcgcgt agcttctcgc
    18001 agaatccgta ctgccctttc gattttcgcc ccattctttc cgaaatcaaa aatacaagga
    18061 tggggaagta ccatccgttc cattaccagc gattttggga agacccgcga cctggatgtt
    18121 cagatcgagt tcctggaaaa ccagctcaat gcgatcagcg acagaaaggt gcgggcaggc
    18181 atcaatcggt tgcagctacg tttaatacaa aaccggaaga agagagaccg taaggtcaag
    18241 aaacacacta cctctcttct taaaagcaaa gatcttaact ctttgttgaa caccatgcag
    18301 cggatatcac aaacacccgg cgaggagaat tatccggttg aactttacca attagccttt
    18361 cacactattt acaacgcact ggatcaattt ttatattatg aagtattcat ccagcatcca
    18421 gaaaatatac acgagttgca tctcatgcga attgctgcaa aaaaagtgcg gtacacgtta
    18481 gaagtgttct tgcctctcta ccagggaaaa atggatgcat ttctggatat catgaaaaac
    18541 atccaacaaa aattgggggc gattcacgat tgcgatgtat ggatcacgtt cattcctgag
    18601 ttcatggaag cggaaaaaca acgcaccttg aaatattacg gtcgatcttc tgccttccat
    18661 cgcttcattc caggacttga attcattaaa aaggatcggc agcaagaacg tgaccgcatc
    18721 tacaccgaat tcatgaacga atggcaaaaa tggcgctctg ctgaaacatg gttgcggatg
    18781 cgagaattaa tcctgcaagc ctcagtttct gttatggata acacacccat caataccgat
    18841 ggcagcaggg gaaacgaaaa aaatgcgaac atttcaggat aggaagattt ccaatcccat
    18901 tcaaaagatc gcattagtca gtgatatcca tgctaaccta cccgcccttg aagccgtata
    18961 tcggcatgcc atccagcatg acgtacagct ctttctcaat gccggagata ccgtagggtt
    19021 cggaccgttc cccaatgaat gtttggattt cattcgcctt aagaagatgg ttagcgtaaa
    19081 gggcgattac gatcaaaaag tgttggaata ccccaaaaga aaaaattctc tggaaaagaa
    19141 gaagaatcct ctgcaattgt tttcattcaa atgggcgtat gagcagttga ccgagcacaa
    19201 cttccgtttc ctggctgatc tgcctgaatt tcaatgcatt cccattggaa tgaaaaaggt
    19261 ctatatcgcc cacggttcac cgatgagcat aaaggctcac cttggttcaa ccacaaccca
    19321 tagcgaattg gtagcgaatc aaaaaattgc ccaaagcgac ttcatcattt gcgggaattc
    19381 acacgtctac tggagttcac aagtgaaaga taccctcttc atcaaccctg gcagcgtcgg
    19441 cagacaggat gatggcgacc cacgggcatc gtatgccaca ctctctctaa gcgaccccat
    19501 caaggtggac cattatcgtg tgccttatga tatttcaaga gtccttcaag cgattgaggt
    19561 ttacaacctg ccggtcgaat tctcttatat gttccaacgg ggggttaatt tggagagagc
    19621 aattgcagaa ctctccaacg caaaaaatga ccagatcagc acagcttgat accaacgatc
    19681 caaagccatc aaaggaatca atcaggctgg cacaaatata atggaacaac ttgtcggaaa
    19741 atgtgcctgg cgcaggtttt tcagtgaggc gctgtcatgt ctcaagatcg aacaggtctt
    19801 ttttaattcg cctggctgat ggttgaggga aatactgcat atatacttca ctggatgaga
    19861 taagacgttc cagccacgca ataaaaaaac cgctctgccg atctcccccc tggctgcgat
    19921 aacaggcaga agctttttct cgcagtacag catattttcc ataatgaata cgcacattga
    19981 tgggaaaatc ggcttccatc acctctacaa aattgatatc tccatttttt ccaaacttcg
    20041 tggggtcttt tcctaccaat ggcatgagct tcaccaccag ccgcatgaac ttgcgaggaa
    20101 aagtgtgcag ataaacgccc gccgggtgaa atggactcat cccctcgata tggattcgct
    20161 catcagccgc taactccaac gctctctctg ttgaacggta tgcatgaatg tgatccggat
    20221 gaccataacc gcccataggg tccgaagtga gcacaacagc aggtctgatt tttcttatgt
    20281 aatatgctat atccatccct gtctgttcca agggtgcctg gataaaagct tgcggatgat
    20341 tgttgtcagg gcttcccggc atgcccgaat cgcggtatcc cagaaagtaa tattcgtgca
    20401 ctcccagaat ctgcagtgca ttgcgcattt cagctactcg cagatcagcc attgaatcat
    20461 actgcatcaa cagctcagca tcag
//
LOCUS       DAOSDS010000036        19988 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1130_length_19988_cov_4.871520, whole genome shotgun sequence.
ACCESSION   DAOSDS010000036 DAOSDS010000000
VERSION     DAOSDS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19988
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1130_length_19988_cov_4.871520"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="PLL88_05835"
     CDS             complement(<1..1689)
                     /locus_tag="PLL88_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01122.1"
                     /translation="MSGIIQSVSSGSIGFLIWCMLWGLGGIFLIRGVFKLAKNEFLMI
                     GLGAGIIIESWLANILGRFLEPLLAFWLAGLIVFFCGMLLCLPLFRKKTDVKVQFPVI
                     LWQWIVFFVLFYVFYMVARGMSIWDEFKTFPLVSMIAAGDIPPHFPYDIRVVLDEHYL
                     RYLIAAQFMRVGELYPWTALAFVQSFTLSLSVVLVITWVYRITKNAVAGMIGGVFHLF
                     AGGTRWLLLLFPPSIIDKIDTVIHRVGSGLNSGSDLRTALISPWAAQGMAPISIPFAF
                     TNGLIKPSVLGVGWDNISFLILILILLLYGKAKDTKTIAIVGVLLAALGLMDEMTFAF
                     LSGLLIVIHLFQLKNKDDEIKKGARQHLTMLIFVWIVVFLQGGFFTAAANNILHPDYT
                     AYHDFRIEFVFPPSFINPHLGELKIINPIHLLVLLVECGPLVLVFPLVLIWGWKSFRN
                     KKYIDAAYIGTAILGLAFCFVQITSKGINTAVNRVQNDFLEVSLLYAVPILYYCIQRR
                     TLKTKLAVGLLALVAVFGGMVVLGTELSGIQEPELGIFITEMDVRMMDKYWNKLD"
     gene            complement(1743..2951)
                     /locus_tag="PLL88_05840"
     CDS             complement(1743..2951)
                     /locus_tag="PLL88_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HOJ01123.1"
                     /translation="MIPRLKPYFNSSEIGAFLHHGRLAVTEFEKRFAEKFQCADAVLF
                     PYGRSALWAFFRSQGIKGKHIVQPAYTCSVVAHATVLSENTPLFVDNNLHDYNMQLDG
                     LEAAISPDTYAVIPTHLFGYPMNVHAVQDIVKTAQERYGHKIWIIQDCAHSFGASFQG
                     TMVVNEGDCALFGLGISKVISSIFGGMMTFQDLRLAEQVRRYRDEHFRVPVWFKTIKR
                     LAYLLATYPAFNDGMYGLVYWMQEKTAVLNSLTRAYHLDEEIRFPPDAKERSLSGEAA
                     VGLVQLEKYDAIIQRRREIAAHYHDQLKVPEDWVLPPLVDGATYSHYAVRVPQREETI
                     QKIAKKGIQAGRVIEYSIPHLASYQPYTGGNKFPNAKLCSQSMLNLPIHPSLRDEQVE
                     QIITCVNTVE"
     gene            complement(2941..3888)
                     /locus_tag="PLL88_05845"
     CDS             complement(2941..3888)
                     /locus_tag="PLL88_05845"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ01124.1"
                     /translation="MSQAAFVSIDVDTLASIYKGIGCRRKGGYSYAEMQIGIENILRF
                     FASYQCSLTLFMVGEDLDFEQNIPPAKELLQAGHEIANHTMHHAQGFRFLSPEQKRAE
                     IYEMEAICQKKLGTKPLGFRSPGWNISDDAGVILKEMNYLYDSSVFPTSFMPLLKFSH
                     WLSMRSKPRIERSTMGQNSYMRAPHQPYCTSLEKFNVAGDDGIMEFPITVSPKLHLPF
                     TATFYLLFGWKFYSKILDGLIKRDVPIHFQFHLSDFVDYTTGEFAEQLPADRQGVYIP
                     QALKMPYAQKEKAFRKIMDVLASNYSFRRMDQWSREHDS"
     gene            complement(3885..5525)
                     /locus_tag="PLL88_05850"
     CDS             complement(3885..5525)
                     /locus_tag="PLL88_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HOJ01125.1"
                     /translation="MKVLSLHDQLTLVCRRVINLQNQDPLSKTRGCFDRRFWAWKLCD
                     YPEATYQRLILPLCWYMHEEKDPFLKQKAYVAVRLGLEYALKIQHSDGSFDQAYPYER
                     SYGATAFLLHPLLKAYRSISAQYEPFERQSFEERLYQSAAYLVKYKEFHSFIANHLAG
                     SALALLEAGLFFNDSRFSHSAQELVDMLISNQSSEGWYLEYDGADPGYQTLCVHYLAQ
                     FYDLNQDPGLLLSLQNAIHFLSHFAHPDGSFGGEYGSRRTAIYYPGGVALLAKTIPEA
                     ASLHDFMLRSIEAGKTVSALEIDDGNLAPLLSSAILTEQENGELASPLEALPCQLAQI
                     DRDFPQAGISIRGNGRYYAVVGISNGGVCKFFAQKDKQLIYNDCGLVLQKKNGKMLTS
                     QHTHLKNSSVSLNKNTISFSVLCSPYARLEQTPATFLLLRIANLTFMRIRFLNELIKK
                     MLVNVLISPSSGGKITRTVKIDFKENAIQIHESLSGRELHNILSITRASAFTAIHMAS
                     ARYPLSTSWIGGQEELPFHETGGVLSREEEFSFSGKKK"
     gene            5653..6717
                     /locus_tag="PLL88_05855"
     CDS             5653..6717
                     /locus_tag="PLL88_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOJ01126.1"
                     /translation="MSRMFVPGPVDVAPEVFAAMSQPVLPHRSKEYEAIYQRAAKKCE
                     DLFYTKYRVFIGTHSGSGMQETGIRNLVRKDVLCCVNGAFSARWYDIAIGNGKQADKL
                     EVEWGKAIEPDQLRDALKKKHYEAVTIVHNETSTGCESPVKDLCAVVREVSPDTLVMV
                     DAVSSLAGTKIEMDAWGIDFLLTSSQKCIALPPGLSLAGTNDRAMEKAKTVENRGWYF
                     DLLIMEEYRTKNSTAMTSAVTLVYGLDFQLDRIFAEGLDKRFARHAAMSKRVYDWSLE
                     RGMEPFADAKYLSKTVATIKNTRELDISKLNAFLLERDMRIANGYGSLKNKTFRIATM
                     GETHMEDVEKLLVSIDDFMK"
     gene            complement(6785..8608)
                     /locus_tag="PLL88_05860"
     CDS             complement(6785..8608)
                     /locus_tag="PLL88_05860"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOJ01127.1"
                     /translation="MSATKMDVEMLLKKSHLQNVQFGEEDLLLWTESDDGVGKAFIRN
                     ANSETRSIDLPDSIKSGLFYGGGEFCTGSGKAIFCVNRNRLVAVDVHSGEKKELIPAW
                     GKVAAPSISPDGKWVLYTFTDGETDLIGIAPVHGLDWPRQMVKGADFYLQPTWHPQGG
                     LIAWAEWDHPCMPWQGSRVKIGAVEGMQIRLFEETWIDGKLGMAAHQPQFSPNGRWLS
                     YLIQNGEWEDLVLFDLKKKEKRVLVRGSGFMLSTPDWVQGLRSYAWESDARNIYYIRY
                     ANAQATLWRVNVRSGVSVQVPVSPYTWLSQLTVSSQSGTLACIASSPYIPKRIVFLRE
                     GKLDEWNPAQKKESLDYFKEPEHIEWKGVDGGSVFGILYLPENERNVPLPLIVDVHGG
                     PTMQRTFSETAEVGFFVSRGYTYLQVNYRGSSGYGLRYQDALEGKWGELEVDDIYSGV
                     KHLIRMGIAAEGRIALMGNSSGGTTVLNVLRKYPGVFRCAVCSFGIGDLITDARQTHK
                     FERFYHRYLIGDLEQKLDVFRQRSPLFHSDEIQDPLLLFHGSEDPVVHPAQTQALYDS
                     LSKRGIPCCLKIFDQEGHGFKREETLREYYTLVLEFLEKYL"
     gene            complement(8672..9160)
                     /locus_tag="PLL88_05865"
     CDS             complement(8672..9160)
                     /locus_tag="PLL88_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ01128.1"
                     /translation="MKKVFLMRHAKSSWKDANIPDHDRPVKKKGEKDVKEMAKMLRHK
                     KHLPDYILCSSAERAKQTAIIFKKASSCEGEIEYSDKLYMAEVPDLIAALKDAPKKTK
                     TVMVIGHNPGLEALLQTLTGKVETLPTSSIAYISLPIEKWSELNSEVEGKLKKLWRPK
                     DL"
     gene            9228..9599
                     /locus_tag="PLL88_05870"
     CDS             9228..9599
                     /locus_tag="PLL88_05870"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HOJ01129.1"
                     /translation="MNKQSLAGRFIGEPIEAIFYQEQIKTKNPGCPDAIRWRSNDYVI
                     SKVINQWTDFSRKGNMRRNMREAHRTRAEEKGSWGVGRIYFKVQVHTGQIFEIYFDRA
                     PQKASDREGNWFLFQEYPCTP"
     gene            9696..10076
                     /locus_tag="PLL88_05875"
     CDS             9696..10076
                     /locus_tag="PLL88_05875"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HOJ01130.1"
                     /translation="MNKVIDKCPICNHELIVTALHCNNCRTNFQGVFELTHTPLSNLS
                     ADQLQFVLTFIRCEGKFNRMEEELDLSYPTLRSRFNEILRVMGFESQEEDDKPSSEAR
                     KEILTKLNEGKISIDEAQELLRGN"
     gene            10127..10483
                     /locus_tag="PLL88_05880"
     CDS             10127..10483
                     /locus_tag="PLL88_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01131.1"
                     /translation="MMILKMLQEGKINADQAAKLLEALEAGKPASSKEENEEKTGKWF
                     RVRVTDAITGIQKTNIRIPIGLVNAGFSIGAQFSPEFAQLNSNDLMNFIKSGKTGLIL
                     DVIDDDDDEHVEIFIE"
     gene            10508..11776
                     /locus_tag="PLL88_05885"
     CDS             10508..11776
                     /locus_tag="PLL88_05885"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ01132.1"
                     /translation="MQNQFNKNWKTKFFSVWLGQAFSLLGSQIVQFTLIWYLTKETGS
                     AAVLAISTFVSLLPNVLLAPFIGALVDRWDRKQIMIISDLLIALVTFLLIILFKTEQV
                     KIGYIYLALFLRELGSTFHWPSMRSSTSLLVPQEQMARVEGMNQTIRGLLNVVGPLLA
                     ALLLEVSPIYELLFIDIITALLAVTPIAFVNIPQPSKKTSEKITSVSDLWSDVKFGYR
                     YLWNWKGMFLLTLTATLLNFLINPGFTLTPLLVTQHFSGGAIQLSLVESIFGIGVIAG
                     GLLLSTWGGFTKKILTSLSGIIGMGTGMLLIAIAPSDQFKLALAGMFITGFMNPIANG
                     SLIAIMQANVKAEMQGRVFTTLNSTASAMTPIAMLIAAPVANTVGIQGWFYIGGLGCV
                     IMGLLGISNPSMMKIEEEGQRRLKEDIQPS"
     gene            complement(11801..12367)
                     /gene="gmk"
                     /locus_tag="PLL88_05890"
     CDS             complement(11801..12367)
                     /gene="gmk"
                     /locus_tag="PLL88_05890"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOJ01133.1"
                     /translation="MFVISGPSGVGKDSAVKALKKRPTKFHFVITANTRAPRADEVEG
                     VDYFFVSKAHFEEMIKNNELVEWAHVYGDYKGVPKSQIEGALASGKDVLMRLDVQGAK
                     KIKSLYPSAILIFLLPSSEEEWRRRLEERAREQGTDVDMDIRIEKMKEELATLGIFDY
                     AVINADGQLNQTVETILAIITAEHHKLV"
     gene            12454..12861
                     /locus_tag="PLL88_05895"
     CDS             12454..12861
                     /locus_tag="PLL88_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ01134.1"
                     /translation="MKKSSKRSLKMPVYQFICLACRVGFEKSISFKEYGTIPISCPHC
                     QSHHVQRKIGKVRINRDAAHADDFSVDPAAFSELEEDPRSMGKMMRKMEEQTGEKFEP
                     EFNEVIDRLEKGQTFEQIEKELPDINPPSSTLS"
     gene            13019..14437
                     /locus_tag="PLL88_05900"
     CDS             13019..14437
                     /locus_tag="PLL88_05900"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ01135.1"
                     /translation="MPAEKKTKIGKKYYYIGGVILALIGILVVAQIRKNAANNDGLLT
                     AVVERGELIATVGGTGKVEANKSATLTWKTSGNVSAIHVKNGDDVSAEDILAELSPAS
                     LPQAVLLAQADLVEAKRNLESVLESNTLRSQTYLDLLKTEETLKEAQKDIDRWNYKNA
                     DQKVIDQARSEFIQAEEALKQAESELQALSTNAREEEIQKTQAKTDEAQATRDKALRN
                     LSYVLGKTYNTEVAEDFANYDLAKAQLEDAQREWDRVKNGQNEDDTLAAQAKVTAAEA
                     VAGMANLRAPFDGTITAIMTKEGDEVSAGVNAFRIDDLSKFYIEVDIPEIDINRIKTG
                     QEAAFTFDSLLDKTYHGTVVEVAGAGTESQGETAFTIKLLMNDADENILPGMTASVSI
                     TVLKLDDVLIVPSRAIRLENDKTVVYVQRNGAIEKVVVAIGSSSDSYTQILSGDINEN
                     DVLVLNPPEDIFSTNQQPVFTR"
     gene            14440..15123
                     /locus_tag="PLL88_05905"
     CDS             14440..15123
                     /locus_tag="PLL88_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01136.1"
                     /translation="MKENVIKIKDLTKVYKMGEVEVHALRGVTLNIQQGEIISIMGPS
                     GSGKSTLMNILGCLDRPTSGKYILDGKNVSNLDDDELANIRNQKVGFVFQNFNLLSRS
                     TALANVELPLRYAGIHEDRMALAQKALENVGLGDRVNHKPPELSGGQQQRVAIARALI
                     NNPAIILADEPTGNLDSKSGKEIMELLLGLNKEYGTTLIIVTHDPKIAKFTARTVHIF
                     DGKIKESRS"
     gene            15120..16349
                     /locus_tag="PLL88_05910"
     CDS             15120..16349
                     /locus_tag="PLL88_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01137.1"
                     /translation="MNFLQIVDEAWSSLNANKMRSILTTLGIVIGVAAVIAMIAIGTG
                     AQDTILNQISGIGTNLLFVFEGNEGSNGSTNVSPLTMQDANAIGEVFAAPHVELVAPV
                     IQGSGIAKYSGETKSTQIFGVTPEFQNIRNYELTEGEFISEENVNGRASVALLGVDVA
                     EKLFGQTENLVGRTITIEGQPFRIIGVLAPKGGGQFGSHDDVILVPISTARSRLFTRE
                     QGKVDIIYIKAISAESVPQATSEVQEVLRKRHRTSLGEDDFTIYSQQDFLTLASSITN
                     IFRIFLSGIAGISLLVGGIGIMNIMLVSVTERTKEIGLRKAIGAKKKDILIQFLAESS
                     LLSMLGGILGIIFGWIISALIGSFAATQGIAFKPQVDLGAIFLATLFSTAVGLFFGIY
                     PANKAANLEPVEALRHE"
     gene            16482..17432
                     /locus_tag="PLL88_05915"
     CDS             16482..17432
                     /locus_tag="PLL88_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HOJ01138.1"
                     /translation="MTARRILLTDGLADTGKAILTSQNIAFDDKKGIDADALLKCIAD
                     YDALIVRGRTKVTPEVFAKANKLKVIGRCGVGVDNINLDAAKEKGVIVVNAPVSTTIA
                     VAELTMALMLAMLREIVKADAKMKADIWCKNEVKGSELHGKTLGIIGFGHIGSTVGIY
                     AKAFGMKVIVYDHNPVTSETQTEKHDNKAVSFDELLQKADIITIHVPLTDCTRHMINA
                     QTIAKMKDGVRIISTARGGVIDEEALLQALESGKVTSVALDVFEKEPPANSPLVKHPK
                     LIATPHIGGETKEAQQRASADIVNEVVAALNGEPLHWRVA"
     gene            17466..18968
                     /locus_tag="PLL88_05920"
     CDS             17466..18968
                     /locus_tag="PLL88_05920"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HOJ01139.1"
                     /translation="MKGKLEQLKNLLHEIHDLAAASAVLDWDQQVNMPRGAAEDRGEQ
                     SATLSRIVHDKSTSDELGDLLNELTAMTQNVDADSDDARLVKVAKRKYEKNTKVSGDW
                     VATFAKETAVAQSTWEQARAKDDFELFRPHLEKLVDLRRQYAEFFKPYTHVYDPLLDD
                     FEPGMKTAEVQEIFTALKKEQVDLIHQISQQPQVDNRFLFLHYDGDGQIAFGKKVITD
                     FGYDWNRGRQDISVHPFTTGFGLNDIRITTKIVPDFLNPCIFGTFHECGHALYEQGIA
                     QKYNRTALADGASMAIHESQSRMWENLVGRSMAFWKRYYKDLQEIFPSQLGNIDVETF
                     YKGINKVESSLIRIEADEATYNLHIMLRLEMEIGLMEGSIQAKDAAEVWKEKFKEYLG
                     IVPPNNKEGVLQDVHWSSGLFGYFPTYALGNLVSSQLWETMLKDNPDVEKQIETGNFS
                     QLLGWYREHIHQYGAKYEPQELVLKVTGQKINAASFMRYLKSKFGKIYNL"
     gene            complement(19202..>19988)
                     /locus_tag="PLL88_05925"
     CDS             complement(19202..>19988)
                     /locus_tag="PLL88_05925"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HOJ01140.1"
                     /translation="LYITYTYQSDVGGKHFVQVESGEWVRASPATYSPFQGLQFTKTP
                     SHSFGWILDQANPRRGPGYQAEVINETLTREQVVQVYDAVEANKTTWYMIGLGKWVEQ
                     RYIGVVNPMSAAPDEKITNGRWIEINLYQQTLAVYDNNQLVFAALIATGADPYFTQPG
                     TFQIYEKKELETMTGSFAADKSDYYYLEDVPWTMYFDQKRALHGAYWRALFGYTQSHG
                     CVNLSMGDSHWLYNWANVGDWVYVWDPSGATPTDPTFYTQGGA"
BASE COUNT         5692 a         4800 c         4543 g         4953 t
ORIGIN      
        1 gtctaattta ttccaatatt tatccatcat gcgcacatcc atttcggtaa taaagatgcc
       61 aagttcgggt tcctggatgc ctgataactc agtgcccaag acgaccattc ccccaaacac
      121 tgcaaccaac gccagcagac cgaccgcaag cttcgttttc agcgtgcgtc tctgaatgca
      181 ataatagaga attggcaccg catacaacag gcttacttca agaaaatcgt tctgcactcg
      241 attgacagca gtgtttatcc cttttgaggt gatttgcaca aaacaaaacg cgagccccag
      301 tattgcggtg ccgatataag ccgcgtcgat atattttttg tttcgaaacg atttccaacc
      361 ccagatcaac accagaggaa ataccaaaac cagcggtcca cattctacga gcaggacaag
      421 caaatggatt ggatttatga tcttcagttc tcccaagtga gggttgatga agctgggggg
      481 aaagacgaac tcgattcgaa aatcatgata ggcggtgtaa tcggggtgca agatattatt
      541 cgctgcggcg gtgaaaaatc ctccctgtaa aaacaccacg atccatacga aaatcagcat
      601 ggttaaatgc tgccttgcac cttttttgat ctcgtcatct ttattcttta actggaaaag
      661 gtggatgacg atcagtaaac cactcaaaaa agcaaatgtc atctcatcca tcaaacccag
      721 cgcagccaac agcacaccaa cgattgcgat cgtctttgtg tctttcgctt ttccataaag
      781 caataaaatg aggatcaaga tcagaaaact gatattatcc caccccactc ccaaaacgga
      841 tggttttatt aaaccgttgg taaatgcgaa tggaatcgat atgggagcca ttccctgtgc
      901 tgcccaggga ctgatcaaag cagtgcgcag atcagatccg ctgttcagac cactgcccac
      961 tcggtgaatc acagtatcaa tcttatcgat aatcgatggt ggaaacagca atagcaacca
     1021 gcgcgttccc cccgcgaaca aatgaaagac tccccctatc attcctgcaa cggcattttt
     1081 cgtgatcctg taaacccatg tgatcaccag gacaacgctc aagctaagcg tgaaggattg
     1141 tacgaatgcc agggctgtcc aggggtagag ttctcccacg cgcataaact gagctgcgat
     1201 gagatagcga aggtaatgtt catcgagcac aacacggata tcataaggaa aatggggtgg
     1261 aatatcccct gcagcgatca tggaaaccaa cggaaaggtt ttgaactcgt cccaaatgga
     1321 catgccgcgc gccaccatgt agaaaacata gaatagcacg aaaaatacta tccattgcca
     1381 gaggataacg ggaaattgaa ctttgacatc ggtcttcttg cggaatagag gcaaacaaag
     1441 cagcatcccg cagaagaaaa cgatcaatcc cgccagccaa aaagccagca aaggttcaag
     1501 aaatcttccc aggatgttcg ccagccaact ctcaataatg atccccgctc ccaacccaat
     1561 catgagaaat tcgtttttcg cgagtttaaa gacgcctctg atcaggaaaa tgccacccaa
     1621 tccccataac atgcaccata ttaaaaatcc tatcgaaccg ctggatacgc tctgaatgat
     1681 tccactcacg cccgctctcc ttttatcaag acgtacactt tattgcttat caacgttccg
     1741 cctcattcta ctgtattgac acaggtaatg atctgctcca cctgctcgtc gcgcaaagat
     1801 ggatggatgg gtaaattgag catactttga ctgcacaatt ttgcgttggg gaatttgtta
     1861 ccgccagtat atggctgata actcgccaga tggggaatgc tgtattcgat gacccgacct
     1921 gcctggattc ccttcttcgc aattttttgt atggtttctt cccgttgcgg aacccgaacg
     1981 gcataatggg aataggtcgc accgtccacc agtggtggaa gcacccaatc ttccgggact
     2041 ttaagctggt catggtaatg agcagcaatc tctctgcgcc tttggataat ggcatcatac
     2101 ttctcaagct gcacaagacc taccgccgcc tcacctgaca acgatcgctc tttggcatca
     2161 gggggaaaac gtatctcttc atccaggtga tatgccctgg taagagaatt caaaacggcg
     2221 gtcttttcct gcatccagta taccaacccg tacattccat cattgaaagc gggataggtt
     2281 gccagcaaat aagccagacg ttttatggtc ttgaaccaca caggaacgcg aaagtgttca
     2341 tccctatatc tgcgcacctg ttcagccaga cgaagatcct gaaatgtcat catcccgccg
     2401 aagatggagg aaatgacctt gctaatcccc agaccaaaaa gagcgcaatc gccttcattc
     2461 accaccatcg tcccctgaaa acttgcacca aaggaatgag cgcaatcctg gatgatccag
     2521 atcttatgac cataacgctc ctgtgccgtt ttgacgatgt cttgcacagc gtgcacattc
     2581 atcggataac caaacaaatg agtcggaatc accgcatagg tgtcaggcga aatagccgct
     2641 tccagaccat caagctgcat attgtaatcg tgaaggttat tatccacgaa cagtggagta
     2701 ttttcgctga gcacggtggc atgcgcaaca acagaacagg tataggcagg ttgaacaata
     2761 tgttttcctt tgatcccttg tgaacggaaa aaagcccaca gcgcgctgcg cccgtagggg
     2821 aacagcactg catcggcaca ttgaaatttt tcagcaaaac gcttttcaaa ttcagtcacc
     2881 gccaggcgtc cgtgatgtag aaaagcgccg atctcgctgc tgttgaaata gggtttcaat
     2941 ctaggaatca tgttccctgc tccattgatc catccgtcgg aaagaataat tgcttgcgag
     3001 cacatccatg atcttgcgaa acgctttttc tttttgtgca tacggcattt ttaaggcttg
     3061 agggatgtat accccttgac gatctgcggg aagctgctca gcaaattccc cagtcgtgta
     3121 atctacaaaa tccgaaaggt gaaattgaaa atggatcggg acatcccgtt tgatcaaccc
     3181 atcaaggatc tttgaataaa atttccagcc aaacagcaaa taaaatgtgg ctgtgaacgg
     3241 aagatgaagt ttcggtgaga cggtaatggg aaattccatg attccatcat cacccgctac
     3301 gttaaatttt tccagggatg tgcagtaggg ttgatgggga gctctcatat aggagttctg
     3361 ccccatggtc gaacgctcga tacggggttt ggagcgcata gaaagccagt ggctgaactt
     3421 cagcaatggc atgaagctgg tcgggaaaac cgaggaatcg tacagataat tcatttcctt
     3481 aaggattacg ccggcatcat ccgaaatatt ccagcccggt gaacgaaacc ctagcggttt
     3541 tgttcctaac tttttctggc agatggcttc catttcatag atctctgccc gtttttgttc
     3601 aggcgagaga aagcggaacc cttgagcgtg atgcatggtg tggtttgcaa tttcatgtcc
     3661 tgcttgaagc agttctttgg caggtggaat attttgctca aagtcgagat cttcgccgac
     3721 catgaacaag gtcaaggaac actgatacga tgcaaagaac cgaagaatgt tttcaatgcc
     3781 aatctgcatt tctgcatagg agtacccgcc cttccgtctg cagccaatac ctttgtaaat
     3841 ggaagccagg gtatctacat cgatcgaaac aaatgccgcc tggctcattt cttcttcccc
     3901 gaaaaagaga actcttcttc tctggaaaga acgcctccgg tttcatgaaa aggcagttct
     3961 tcttgccccc caatccagga agtgctcaaa gggtagcgtg ctgacgccat gtggatggcg
     4021 gtaaaagcgg atgctctggt gatagacagg atgttgtgca actctctccc gcttagcgat
     4081 tcatgtattt gaatggcatt ctctttaaaa tcaattttca cggtccttgt aatttttcca
     4141 ccagaagaag ggctgatcaa tacgttcacc aacattttct ttatcagttc attcaaaaaa
     4201 cggatgcgca tgaaagtaag atttgcaatg cgcaacaaaa gaaacgtagc cggtgtttgt
     4261 tccaaccttg cataggggct acagagtact gagaatgaaa tcgtattctt gttgagcgat
     4321 acggaagaat tcttcaaatg ggtatgctgc gatgtcaaca tttttccgtt tttcttttgg
     4381 agcaccaatc cacaatcgtt atagatcagt tgtttgtcct tttgagcgaa gaatttacag
     4441 actcccccat tggatatgcc gaccacagcg tagtagcgac catttccccg aatagaaata
     4501 ccggcttgcg gaaaatcacg gtcgatctga gcaagttgac agggcaaagc ttcgagcgga
     4561 gatgccagct ctccgttttc ctgttctgtc aggatggcac ttgaaagaag gggagccaga
     4621 ttgccgtcat cgatctcaag agcactgacc gtttttcctg cttcgatcga gcgcagcatg
     4681 aaatcatgca aacttgctgc ctctgggatg gtctttgcca gcagagccac gccacccgga
     4741 taatatatgg cagttcgcct gcttccatac tctcctccaa aacttccatc agggtgagcg
     4801 aaatggctca gaaaatggat ggcgttctgc aaagataaaa gcaatccagg atcctggttc
     4861 aaatcataga attgagccag ataatgaaca cagagggtct gataccctgg atcagccccg
     4921 tcatattcca gataccatcc ttcagaagat tgatttgaaa tgagcatatc aaccagttcc
     4981 tgcgcagaat gcgagaacct ggaatcgttg aaaaataaac cggcttccaa caaagccaga
     5041 gcactaccgg ctaaatgatt ggcaataaaa ctatggaatt ccttgtactt taccagatac
     5101 gcggcgctct gatacaggcg ctcttcaaaa gattgtcttt cgaatggctc atactgtgcg
     5161 gaaatagatc ggtatgcttt caataagggg tggagcaaaa acgccgtcgc tccataactg
     5221 cgctcataag gataagcctg atcaaaactg ccatcgctgt gctgtatctt caacgcatat
     5281 tccaacccta accgaacggc tacatacgct ttttgtttga gaaatggatc tttttcctca
     5341 tgcatgtacc aacataacgg cagtatcaac cgctgatagg tggcttcggg gtaatcgcat
     5401 aatttccacg cccaaaaacg ccggtcaaaa caccctctgg ttttcgaaag cggatcctga
     5461 ttttgtaaat tgatcaccct gcggcaaaca agggtcagtt ggtcatgaag agagaggact
     5521 ttcatagtcg tatgattttt attttcaccg gataggcaat tgcatactag ctaaagcatt
     5581 actatcatat tatcattatt ttgtttcgtc atcagatatc cctcgcaggg aaaaaagaat
     5641 taggagagaa aaatgtcacg aatgttcgtt cctggacccg tagatgttgc acccgaagtg
     5701 ttcgctgcaa tgtctcagcc tgttcttccc caccgcagca aggagtatga agcaatttat
     5761 caacgtgcag cgaaaaaatg tgaagatctt ttttatacaa aataccgcgt tttcattggc
     5821 acccattccg gcagcggcat gcaggagacc ggaattcgca acctcgtacg gaaggatgtt
     5881 ctttgctgtg tgaatggtgc tttttctgct cgctggtatg atatagcgat cggcaatgga
     5941 aaacaggcag ataaactaga agttgagtgg ggaaaagcaa tagagcccga ccagctacgc
     6001 gatgcgctca agaaaaaaca ttacgaagca gtcaccatcg ttcacaatga aacatccaca
     6061 ggctgtgaaa gccccgtgaa agacctgtgc gcggtggtca gagaagtgag ccctgatacg
     6121 ctggtgatgg tcgatgcagt ctcgtcattg gctggcacga agattgaaat ggacgcctgg
     6181 ggcattgatt tcttattgac ctcctcgcaa aagtgtatcg ccctgcctcc aggtctctct
     6241 ctggcgggca ccaacgatcg agccatggaa aaagccaaga cagtcgaaaa ccgcggttgg
     6301 tattttgacc tgttgatcat ggaagaatac cgcaccaaaa actccacagc catgacgtca
     6361 gcggtaacat tggtctatgg tcttgacttc cagcttgatc gcatttttgc agaaggttta
     6421 gataagcgct ttgcccgtca cgcagccatg tcgaaacgcg tgtacgactg gtcattggaa
     6481 cgcggtatgg aacccttcgc agatgcaaaa tatctctcca aaaccgtcgc aaccatcaag
     6541 aatacccgcg aacttgacat ttccaaattg aacgcgttcc ttcttgagcg agatatgcgc
     6601 attgccaatg gatatggcag tttgaagaat aaaacattcc gcatcgccac catgggtgaa
     6661 acacacatgg aagacgtaga aaaattatta gtgagcatcg atgacttcat gaaatagggg
     6721 aacaacaaaa taacaaagag cccaccagaa gtgtgaactg caccccaaaa gtgggctttt
     6781 tgctttaaag atacttttct agaaattcaa ggaccagcgt gtaatactct cgcaaggtct
     6841 cttctcgttt gaaaccgtga ccttcctggt caaaaatttt cagacaacag ggtatccctc
     6901 gcttggacaa tgaatcatac agcgcttggg tttgggctgg gtgcaccacg gggtcttcgc
     6961 tcccatgaaa aagcaagagc ggatcttgaa tttcgtcact gtgaaacagc ggagaacgct
     7021 gccggaagac atccagtttc tgttcgaggt caccaatcag ataccggtga taaaaacgtt
     7081 caaatttatg agtctgcctg gcatccgtta tcaggtcgcc aattccaaaa ctgcaaaccg
     7141 cgcaacgaaa cacacctgga tattttcgca atacgttcaa aacggttgtg ccgcccgagc
     7201 tgttacccat caacgcaatt ctgccttcgg cggcaatgcc catacggatc aagtgcttga
     7261 cgcccgaata gatatcatcc acctccaatt caccccactt tccttccaac gcgtcctgat
     7321 agcgcaagcc gtagccactg ctgccacgat aattcacctg aaggtaagta tatccacgcg
     7381 agacgaaaaa ccccacctca gccgtttcgg aaaaagtgcg ctgcatagtg ggtcctccat
     7441 gcacatccac aatgagcggc aggggcacgt ttcgttcatt ctcgggcaga tacaggatgc
     7501 cgaatacgct ccccccatcc acccccttcc attctatatg ttccggttcc ttgaagtaat
     7561 caaggctttc cttcttctgg gcagggttcc attcatccag tttgccctcc cgcagaaaaa
     7621 cgatgcgctt ggggatatat ggcgaagatg cgatgcaagc caatgtgccc gactgggatg
     7681 aaacggtaag ctgcgacaac caggtatatg ggctcacggg cacctgcaca gacacaccgc
     7741 tgcgcacatt cacccgccag agtgtggctt gtgcatttgc atatcgaatg taatagatat
     7801 tgcgcgcatc actttcccag gcataggatc gcaacccttg cacccaatca ggggtggaga
     7861 gcatgaatcc acttccacgc acaagcacac gtttttcttt tttcttcaag tcgaacagaa
     7921 caagatcttc ccactctcca ttttgtatca ggtaactcaa ccagcgccca ttgggtgaga
     7981 actgtggttg atgagctgcc ataccaagtt ttccatcaat ccaggtttct tcaaacaaac
     8041 gaatctgcat cccttctact gcaccaatct tgacacggct gccctgccag ggcatgcagg
     8101 ggtgatccca ttcagcccag gcaatcaacc ctccctgcgg atgccaggtt ggttgtaaat
     8161 agaaatctgc acctttgacc atctgccttg gccaatccag cccatgaaca ggcgcaattc
     8221 cgatcaaatc cgtctcacca tcggtaaacg tatagagcac ccattttcca tccggagaaa
     8281 tggaaggtgc ggctaccttg ccccatgccg gaatgagttc tttcttctca ccgctgtgca
     8341 catcgactgc cacgaggcgg ttgcggttca cacaaaaaat agcttttcca cttccggtac
     8401 aaaattcccc tcccccatag aacagaccac tttttatcga atctggcagg tcaatggatc
     8461 gggtttcact gtttgcattc cgtatgaacg ccttccccac cccatcgtcg ctttcggtcc
     8521 acaggagcag gtcttcctcc ccaaactgaa cgttttgcaa atggcttttc ttcaaaagca
     8581 tttccacatc cattttcgtt gcactcatgc tgttttcctt atttgataat taaaaaatgc
     8641 tcacggttcg tgagcatctg ttggataagt actagagatc tttcgggcgc cagagcttct
     8701 ttagcttccc ttctacttcg gaattgagtt ctgaccattt ctctatgggt agggaaatat
     8761 acgcaatcga ggaagtgggc agagtttcta ccttcccagt aagggtttgt agcaacgctt
     8821 ccaaacccgg attatgccca atgaccatga ccgtcttggt ttttttggga gcatctttca
     8881 aggcagcaat caggtcgggc acttcagcca tatacaattt atcggaatat tcaatctccc
     8941 cttcacaaga ggaagccttt ttaaaaatga tggcggtctg ttttgctcgc tctgccgatg
     9001 aacagagaat ataatctggc aaatgctttt tatgtctcag cattttcgcc atttctttca
     9061 catctttctc accttttttc ttgacaggtc tgtcatgatc cggaatattg gcatctttcc
     9121 agctcgattt cgcatgacgc atgagaaaca cttttttcat acggacctcc agaacgaaat
     9181 gataaaatat gaaatcgagg atagtataac atatttgatt ttttcctatg aataaacagt
     9241 cgctggcagg gcgcttcatt ggggaaccta ttgaagcgat cttttatcaa gaacagatca
     9301 agaccaagaa tccggggtgt ccagatgcga ttcgttggcg ttcgaacgac tatgtgataa
     9361 gcaaggtcat caatcaatgg accgattttt cacgcaaagg caatatgcgc aggaatatgc
     9421 gtgaagctca tcgcaccagg gctgaagaaa aaggttcttg gggagtcgga aggatttatt
     9481 ttaaagtcca ggttcacacc ggacaaattt tcgaaattta cttcgatcgg gcaccccaga
     9541 aagcctctga tcgggaggga aactggttct tattccagga atacccttgc accccatagg
     9601 aacaaaataa atattattca tcagcacatc gatattacgc aaataatgat atgattacat
     9661 caattatatt gatcacaata taaagcaggt gaaaaatgaa caaagttatt gataaatgtc
     9721 ctatctgtaa tcatgaactt atcgtcaccg cactacattg caataattgc agaaccaact
     9781 tccaaggggt gtttgaactt acccacactc cactgagcaa tctctctgct gatcaactcc
     9841 aattcgtact gaccttcatc cgatgcgaag gaaaattcaa tcgcatggaa gaagaactag
     9901 acctttccta cccgactttg cgcagccgat ttaacgagat tctgcgcgtg atgggtttcg
     9961 aatcacaaga agaagatgac aaaccttcca gcgaagccag aaaagagata ttgaccaaat
    10021 tgaacgaagg gaagatctcc atcgatgaag cccaggaact attacgcggc aactagaaaa
    10081 acggattaga attagacagg agcaggatac catggaagaa gatcgcatga tgattctaaa
    10141 aatgctgcaa gagggaaaga tcaacgccga ccaggcagca aaacttcttg aggcactaga
    10201 agccggaaaa cccgcaagtt ccaaagaaga gaatgaagaa aagacaggca aatggtttcg
    10261 tgtgcgtgtt actgatgcaa taaccggcat ccaaaaaacg aacatccgca ttcccatagg
    10321 tcttgtcaat gcagggttta gcattggcgc ccaattctca ccagagttcg cgcaactgaa
    10381 ttcaaatgat ttaatgaact tcattaaatc aggaaagacc ggtttgatcc tcgatgtgat
    10441 tgatgatgac gatgatgaac atgtagaaat cttcatcgaa tagcggcagt cagcataggg
    10501 gatattcatg cagaatcaat tcaacaaaaa ctggaaaaca aaatttttct ccgtctggct
    10561 cgggcaagcg ttcagtttgc tcggttctca gatcgtccaa ttcactttga tctggtatct
    10621 caccaaagag acagggtcgg cagccgtgct ggctatctcc acctttgtaa gtctattacc
    10681 gaatgtgctc ctggctcctt tcatcggcgc attggtagac cggtgggatc gcaagcaaat
    10741 catgatcatt tctgatctct tgatagccct ggttactttt ttactgatca tcctcttcaa
    10801 aacagaacag gtcaagatcg gatatatata ccttgccctc ttcttacgcg aattgggaag
    10861 cactttccat tggccctcca tgcgctcttc cacttcactg ctggttccgc aagaacaaat
    10921 ggcaagggtt gaagggatga atcagaccat tagaggtttg ttgaatgtgg taggtccact
    10981 cctggctgcc ctgctcttgg aagtctctcc gatctatgaa ctgcttttca ttgacattat
    11041 tactgctctg ctcgcagtga cccccatcgc tttcgtcaat atcccccaac cttccaaaaa
    11101 gaccagcgag aaaatcactt ccgtcagcga tttatggagc gacgtgaaat ttggttatcg
    11161 ctatctctgg aattggaaag gcatgttcct gttgaccctc accgcaaccc tgctcaattt
    11221 tctgatcaat cctggtttta cgttaactcc cctgctcgta acccaacact tctcaggagg
    11281 agccatccaa cttagcttgg tcgaatctat ttttggaatt ggggtaattg ccggcggact
    11341 tcttctaagc acctggggcg gttttacgaa gaagatcctg acctccctct caggaattat
    11401 cggaatggga acgggcatgc tgcttatcgc cattgcgcct tccgaccaat tcaaactggc
    11461 gctggcgggc atgttcatca ccgggttcat gaaccccata gccaatggtt cccttatcgc
    11521 aattatgcaa gcaaacgtga aggcagagat gcaaggcagg gtttttacca ccttgaacag
    11581 cacagccagc gccatgaccc ccatcgccat gctgattgca gcgccggttg caaataccgt
    11641 cgggattcag ggttggttct atatcggtgg tttgggctgc gtgattatgg gattgcttgg
    11701 aatctccaat ccttctatga tgaaaatcga agaagaggga cagcgccggt taaaagaaga
    11761 tattcaacct tcataatctc ataaagacga agagggaata ttaaaccagt ttgtgatgtt
    11821 cagcagtgat gatcgccagg atcgtctcaa cggtctgatt taactgacca tccgcattga
    11881 taaccgcgta atcgaaaatg ccgagggtcg ccaactcttc tttcatcttc tcgatgcgaa
    11941 tgtccatatc tacatccgtg ccttgctctc tcgccctctc ttccagtcgt cttctccatt
    12001 cttcctcgct ggacggcaaa agaaaaatga gaatggcaga gggatagaga cttttgatct
    12061 ttttcgcccc ttgcacgtcc aaacgcatca atacatcttt tccgcttgcc agggctcctt
    12121 cgatctgact cttgggcacg cctttataat ctccgtatac atgcgcccat tccaccaatt
    12181 cattattctt gatcatctct tcgaaatgag ctttgctgac gaaaaagtaa tccacccctt
    12241 ccacttcatc agcgcgtgga gctctggtgt tcgccgtgat cacaaaatga aatttcgtgg
    12301 gtcgtttttt taacgctttt accgctgaat ctttcccgac ccctgacgga ccggaaataa
    12361 caaacacgag agccccctca cggagaatat gaaaatttac tttttctgca agagtcatgg
    12421 ataaaatcca atggaagttt aaagtgatta taattgaaaa aatcttcaaa acgcagtttg
    12481 aaaatgcccg tttatcaatt tatctgcctc gcttgccgtg ttggctttga aaagtccatc
    12541 tcattcaaag aatatggaac cattccgatc tcttgtccac actgccaatc acatcatgtg
    12601 caaagaaaga tcggaaaggt ccgcatcaac cgtgacgccg cccatgccga tgacttctct
    12661 gttgatcccg cagcgttctc agaattagaa gaagaccccc gttcgatggg gaagatgatg
    12721 cgcaaaatgg aagagcaaac cggtgaaaaa ttcgagccgg agttcaacga agtaatagac
    12781 cgattggaaa aagggcagac tttcgaacag atcgaaaaag aattgcccga tatcaatccg
    12841 ccctcaagca ctctcagcta acttttctgc caagaaattc ttttcccgtt tcccctttct
    12901 aataaacttt ttacgcattt ccgcttgaca aaagggacag cgaaacttat gtttataccg
    12961 ttccgtaagt attcaataga aaaacggctg ttttatgttg acacaaggaa agggaaaaat
    13021 gcctgctgaa aaaaagacaa aaattggcaa gaaatattac tatatagggg gagtgatcct
    13081 tgctctgatt gggatattgg ttgttgcgca aatacgcaag aatgcagcaa acaatgatgg
    13141 tttgcttacc gcggtggtcg aacggggcga gttgatagcg acggtaggtg gaaccggtaa
    13201 agtggaagcg aacaaatccg ctaccctgac ctggaaaacc agcggtaacg taagcgcaat
    13261 ccacgtaaaa aacggtgatg atgtttcggc agaggatatt ctggctgaat tatctccagc
    13321 ctctttgcct caagcggtcc tattagccca ggcagatctg gtggaggcga aaaggaatct
    13381 cgaaagtgtt ctcgaatcga atactttgcg ctcccaaacc tacctggatc ttctgaaaac
    13441 tgaagagaca ttgaaggaag cacaaaaaga tatcgaccgc tggaactata aaaatgctga
    13501 tcagaaagtc attgatcaag ccagatctga attcatccag gctgaagaag ctttgaaaca
    13561 ggctgaatca gaactgcagg cattgagcac caatgcccgt gaagaagaaa tccaaaaaac
    13621 ccaggcgaaa actgatgaag cgcaagcaac gcgggataaa gccctgcgca atttaagcta
    13681 cgtcttagga aaaacataca atacggaggt cgcagaggat ttcgctaatt atgacctggc
    13741 gaaagctcaa ctggaggatg cgcagagaga atgggataga gtcaaaaatg ggcaaaacga
    13801 ggatgatact ctggcggcac aggcaaaggt gaccgctgcc gaagcggtgg cagggatggc
    13861 gaacctgcgc gcgcccttcg atggcaccat caccgcaatc atgacaaaag aaggggatga
    13921 ggtttccgca ggcgtcaatg catttcgcat cgatgatctt tcaaaatttt acattgaggt
    13981 agatataccc gagatcgata tcaaccgcat caagaccggt caggaagctg cgttcacttt
    14041 tgattccctg ctcgataaaa catatcatgg gacagtggtt gaagtggctg gtgcagggac
    14101 agagagccag ggagagacag ccttcaccat caaattactc atgaacgacg ccgatgaaaa
    14161 catcttgcct ggcatgaccg catctgtgag catcaccgtt ctgaaactgg atgatgtgct
    14221 gattgtgccc agccgggcta tccgactgga aaatgataaa actgttgtat atgttcaacg
    14281 caatggggca atcgagaaag tggttgttgc gatcggttct tcctcagata gctataccca
    14341 gatcctttct ggcgatatca atgaaaacga tgtgctagtg ctcaatcccc ctgaagatat
    14401 tttcagcacc aatcaacaac ctgtttttac gaggtaagcg tgaaggaaaa cgtaatcaag
    14461 atcaaggatc taaccaaggt ctacaaaatg ggagaagtgg aggtgcacgc tttgcgcggc
    14521 gtcacgctaa acatacagca aggtgaaatc atctccatca tgggaccctc cggttcaggg
    14581 aaatccacgc tcatgaacat cctgggttgt ttagaccgtc caaccagtgg caaatacatt
    14641 ttggatggta aaaacgtttc caaccttgat gatgacgagc tggctaacat ccgcaaccaa
    14701 aaagtaggtt ttgtcttcca gaatttcaat ctcctctccc gttcaactgc ccttgcaaat
    14761 gtggagctac ctttaaggta tgcaggaatt catgaagatc gcatggcgtt agcccagaaa
    14821 gcgctggaaa acgtcggatt gggcgaccga gtcaaccata aaccccctga actttcagga
    14881 gggcagcaac aacgggttgc tatcgccaga gcgcttatta ataacccagc catcatcctg
    14941 gctgatgaac caaccggaaa ccttgattcg aaatctggca aggagatcat ggaattgttg
    15001 ttgggattga acaaagaata tggaacaaca ctcatcattg ttacccacga tccaaagatc
    15061 gccaagttca cagccagaac ggttcacatc ttcgatggca agatcaagga gtcaagatca
    15121 tgaatttcct gcagatcgtc gatgaagcat ggagcagcct gaatgcaaat aaaatgcgtt
    15181 ccattctgac caccttggga attgtcattg gggtggcagc agtcatcgcg atgattgcca
    15241 ttggaaccgg agcacaggac acaatattga atcagatcag cggcattggc acaaatctgc
    15301 tctttgtctt cgaaggaaac gaaggaagta atggatcaac gaatgtaagt ccactgacca
    15361 tgcaggatgc aaacgcgatc ggtgaagtat ttgctgcgcc gcatgtagaa ctggttgcgc
    15421 cggtaattca gggaagtggc atcgcgaaat atagcggaga aacgaaatca acccagatct
    15481 tcggagtaac ccccgaattt caaaacatac ggaactatga attaaccgaa ggggaattta
    15541 tcagcgaaga gaacgtcaac ggtcgcgcct ccgtagcttt actcggggta gacgttgcag
    15601 aaaaactgtt cggtcagaca gaaaatctgg ttggaagaac catcaccatt gaaggtcaac
    15661 cctttcgcat catcggcgta ctcgctccaa aaggaggggg gcaattcggc agccatgatg
    15721 atgttattct ggtgcccatc agtaccgccc gcagccgcct gttcacccgc gaacagggaa
    15781 aagtagatat tatttacata aaagcgatca gcgctgaatc tgtgccccag gcaaccagcg
    15841 aagttcagga agttttaaga aaaagacatc gtacgagtct gggtgaagat gatttcacaa
    15901 tctactcgca acaagatttt ctgactctgg cgagcagcat tacgaacata tttcgaattt
    15961 tcctgagcgg catcgccggc atttctttgc ttgtgggtgg cataggaatc atgaatatca
    16021 tgcttgtgtc cgtcactgaa cgaacaaagg aaatcggttt gcgcaaggct atcggtgcaa
    16081 agaaaaaaga tatcctgatt caatttcttg cagaatcttc tttgctcagc atgttgggag
    16141 ggattctggg gatcattttc ggatggatca tctcagcgct gatcggctcc tttgctgcaa
    16201 cccaagggat cgccttcaaa ccgcaggttg atcttggtgc aatcttcctc gccactcttt
    16261 tttccacagc ggtggggttg ttcttcggga tttatccggc aaacaaagcc gccaacctgg
    16321 aaccggtcga agccctcaga catgaatgac gaaaagcagc ggatgtaccg ctgctttttt
    16381 tatatggatg cctgtatgaa gaaaatcggg caactagaca ctattgcagg ggatgggaat
    16441 gcgttacaat ttcccatcat atctcaatta aggagtggag catgaccgcg cggagaattc
    16501 ttctaacaga cgggttagct gatacaggta aagcaatctt aacttcccag aacatcgcat
    16561 ttgacgacaa aaaaggcatt gatgccgatg ctttattgaa atgcatcgca gattacgatg
    16621 cattgatcgt tcgaggaagg acaaaagtta cacctgaagt cttcgcaaaa gcaaacaagc
    16681 tgaaagtaat tgggcgctgt ggtgttggcg tcgataacat caatcttgac gcagcaaaag
    16741 aaaaaggagt gattgtcgta aatgcgcctg tttccacaac cattgcggta gcagagctta
    16801 ccatggcatt gatgctggct atgttgcgcg aaattgtaaa agctgatgcg aaaatgaagg
    16861 cggatatctg gtgcaagaac gaggtcaaag gcagcgaatt acatggaaaa acactgggta
    16921 ttattggctt tggtcacatc ggttcaaccg ttggaatata tgcaaaggca ttcggcatga
    16981 aagtgatcgt atatgaccat aacccggtca catcagaaac acagaccgaa aaacacgaca
    17041 ataaagctgt cagttttgat gaactcctgc aaaaagcaga tatcatcacc atccacgttc
    17101 ctttaaccga ctgcacccgg catatgatca atgcgcaaac gatagcaaaa atgaaagatg
    17161 gtgttcgcat catttcgacc gcgcgtggcg gtgtgatcga tgaagaagct ctgctacagg
    17221 cattagaaag cggaaaagtc accagcgtag cgctggatgt ttttgaaaaa gaacctccag
    17281 cgaattctcc attagtaaaa caccctaaat taattgccac acctcatata ggcggagaaa
    17341 caaaagaagc ccagcagaga gcatctgcgg atattgtcaa tgaagttgtg gcagcgctga
    17401 atggtgaacc cctccattgg agagtagcat aagatcacac tatatagtac ctataaggag
    17461 aaaacatgaa aggcaaatta gaacaattga aaaacctgct gcatgagatc cacgacctgg
    17521 ctgctgcgag cgcggtactg gactgggacc agcaggtcaa catgccgcgt ggggcagccg
    17581 aagaccgagg cgaacaatcg gcaacattga gtcgcatcgt ccatgacaaa tcaacgtccg
    17641 atgagcttgg cgaccttctg aatgaactaa ccgcaatgac ccaaaacgtc gacgccgatt
    17701 ctgacgatgc ccgcctggta aaagtggcaa agagaaagta cgaaaaaaac accaaagtga
    17761 gcggtgattg ggtcgcaact ttcgccaaag agaccgcggt cgcccaaagc acatgggaac
    17821 aggcaagagc caaggatgat tttgaactct tccgtcccca cctcgagaaa ttggtagatc
    17881 tgcgacggca gtatgctgaa ttcttcaagc cgtataccca tgtgtacgat ccactgttgg
    17941 atgattttga accaggtatg aaaaccgctg aagtgcaaga gattttcaca gcactgaaga
    18001 aagagcaggt cgatttgatc catcagatca gccagcagcc acaagttgac aatcgtttct
    18061 tattcctgca ttacgatggc gatggacaaa tcgcctttgg taaaaaggtt atcacggact
    18121 tcggttatga ttggaatcgc ggtcgccagg atatttcggt ccatcctttt acaaccggat
    18181 tcggtttgaa cgatattcgc atcaccacca agatcgtacc tgacttttta aacccctgta
    18241 tatttggcac attccatgag tgcgggcacg ccttatacga acagggaatt gcccaaaaat
    18301 acaaccgcac tgcgctggct gatggcgctt ctatggcaat tcacgaatcg cagtcacgca
    18361 tgtgggaaaa cctggttgga cgttccatgg cattctggaa aagatattat aaagacctgc
    18421 aagagatctt ccccagtcag ttgggaaaca tagatgtgga aactttttat aagggcatca
    18481 ataaggtcga atcctcactc atccgcattg aagctgacga agcaacctac aacctgcata
    18541 tcatgttgcg ccttgaaatg gaaattgggc ttatggaagg gagcattcaa gccaaagatg
    18601 ctgccgaagt ctggaaagaa aaattcaagg aatatctggg catcgtgccg ccgaacaaca
    18661 aagagggtgt cttacaggat gtgcattggt catctggatt gtttggatat ttcccgacgt
    18721 acgctttagg gaatctggtt tcttctcagt tgtgggaaac aatgctgaaa gataacccgg
    18781 atgtcgaaaa gcagatcgag acaggcaatt tttctcaact gcttggatgg taccgcgaac
    18841 atattcatca atatggcgca aaatatgagc cgcaggaact ggtattgaaa gtgaccgggc
    18901 aaaaaatcaa tgccgcctcc tttatgcgct accttaagag caagtttgga aaaatctata
    18961 atttgtagag tttccccata agtaacatca aggagctgtc taaaaagtag acagctcctt
    19021 ttattttttc cactttagcc cgaaaatagg gaaattttgg ggcagaaggg taaaattctc
    19081 ttcttttttg agtattgttt cccactttgc gctgtctaaa aagtaaaaat cagccttttg
    19141 ggacagcctc ttgatgtttt ttcaactcca gcagagcatt cgcttcaaat tgatcgcctg
    19201 cctaggcgcc accctgcgtg tagaaagtcg gatcagtagg agttgcccca ctgggatccc
    19261 acacgtagac ccaatccccc acgttcgccc aattatagag ccagtgagaa tcacccatcg
    19321 atagattgac acacccatgt gattgggtat acccaaacaa cgcccgccag taagccccat
    19381 gtaatgcccg tttttggtca aaatacatgg tccagggaac gtcttccagg taataataat
    19441 ctgatttgtc tgcggcaaag gaaccggtca tggtctccag ttcttttttc tcatagatct
    19501 ggaaggtgcc tggctgcgta aagtaagggt cagcgccggt ggcaattaat gcggcaaaca
    19561 caagttgatt attatcataa accgccagtg tttgttggta gaggttgatt tctatccagc
    19621 gtccattggt gatcttctca tctggcgcag cgctcatggg attgaccaca ccgatatagc
    19681 gttgttccac ccactttccc aaaccgatca tataccaggt agttttgttc gcttccacag
    19741 catcatagac ctgcaccacc tgctcacgtg tgagggtttc gttgattact tcagcctgat
    19801 aaccaggtcc tcggcgcgga ttggcttgat caagaatcca cccaaacgaa tgggatggcg
    19861 tcttggtaaa ctgcaaccct tgaaaagggg aataggtagc aggtgaagcc ctgacccatt
    19921 caccactctc aacctgcaca aaatgctttc cgcccacatc tgactgataa gtatacgtaa
    19981 tatacagc
//
LOCUS       DAOSDS010000037        19500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1163_length_19500_cov_4.157058, whole genome shotgun sequence.
ACCESSION   DAOSDS010000037 DAOSDS010000000
VERSION     DAOSDS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19500
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1163_length_19500_cov_4.157058"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PLL88_05930"
     CDS             <1..257
                     /locus_tag="PLL88_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ01141.1"
                     /translation="WVFLLGLIPQVIGHTLFNVAVRQMDAVYASIALLGEPIGSIILA
                     WIFLSEKPGRYDLLGGLFVIAGILLAFLYKPVSDTDHTTG"
     gene            complement(229..1722)
                     /gene="malQ"
                     /locus_tag="PLL88_05935"
     CDS             complement(229..1722)
                     /gene="malQ"
                     /locus_tag="PLL88_05935"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOJ01142.1"
                     /translation="MKFDRSSGVLLHVSSLPGADGIGDLGKPAFDFIDFLAASRTRLW
                     QVLPLNPTGFGNSPYQGLSSTAGNPLFIALDDLVTMGLLTRHDLEKRPRFPRTKVDYG
                     MVIPWKSAKLQQAFVTFKQNQPVALVNQFKSFCRKHKSWLDDFALFMTIKNQHELHPW
                     NQWSKELRFRDPLGLKNFQKENRDQIEEQKFQQFLFFTQWFKVKAYANKKGVQIIGDI
                     PIFVGYDCADTWTNPSLFFFDKDLKPTVVAGVPPDFFSKTGQLWGNPLYDWKKHKISG
                     YAWWIERIRQTLNLVDVIRLDHFRGFAGYYEIPAGSKTAEHGQWVQGPGKDLFDALYK
                     HFGDLPFIAEDLGVMTPDVIALRERYHLPGMKIVQFGFNPNPEKGFIPHNFDRNAVAY
                     TGTHDNSTALGWFRSVPPTSREHFRAYVGDSRESVPHAMIRMLWQSVALFAIAPMQDL
                     LELGDEARMNYPGSLGNNWEWKLDKKWNAKKLQNWLLGINQLYDRCR"
     gene            complement(1712..2968)
                     /locus_tag="PLL88_05940"
     CDS             complement(1712..2968)
                     /locus_tag="PLL88_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ01143.1"
                     /translation="MSTRAVILAGGEGTRLGVLTAKRAKPAVPFAGKYRIIDFPLSNC
                     VNSSIFDVTIITQYLPQSLIHHIGSGGPWDLNREFTGGIKIFTPFKAKGINWFSGTAD
                     AVLQNLNYILSNDPEYVLVLSGDQVYSMDFRALLRFHQEMDAEVSVCVHSVPLEQASR
                     FGVIETDDQMRIQKFTEKPEKPTSVLANMGIYLFNAKVLKKYLLLDEEDQTSQHDFGR
                     DVLPAIVQEHARIFAFPYRDYWVDVGTIQTYWKAHMDLLSDPPLYDIRKRSWVIHTRS
                     EERPPAYIHPGAYVNASLICDGCIIEPDVEIKNSVISPGVIVQRGTKINESIILTDTM
                     ISPNASIQKAIIDKHVFIGKHCVIGSALKKKNHISMVGKNAILPEGVQLEGGGMVGCD
                     VAPRDFSSLTIHFGEVILPKGNKDEI"
     gene            complement(2972..4396)
                     /locus_tag="PLL88_05945"
     CDS             complement(2972..4396)
                     /locus_tag="PLL88_05945"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOJ01144.1"
                     /translation="MAAEAAPLVKIGGLGDYVGSLPKALLETAQNNNADVSFDIRIAV
                     PYTKMMDSRFLDKTASCSFTSRYARKINHYQVYTTTLDSLIYYVLDNTGLEDQQDIVY
                     TTNPAEDGEKFIAFSLACLDLMEALDWKADIIHANDWQTAAVCSAVKHSQDAAWKRTK
                     SIFVIHNLPYMGAGAEPFIQKYEVPAAHTNAVPAWGKDLPLVIGIEHADRVITVSPRY
                     AQELLTPQFGNDLQEYLSTRKQKISGILNGIDEKFWDPQTDCFIQKNFNAHQLEARKE
                     NKVFIQNALGLAVEPDVPLLVMVSRLDRQKGIRLLCDTLRNFKSEKWQAVILGTGNHD
                     QEELVHALEQAHPQTIRFLNRFDAAFSHLLPAGGDMFLMPSLYEPCGISQMIAMHYGC
                     IPIAHAVGGLIDSIDENETTRTGFLFHNPQRADFLEALNQALQHYHNKIEWGKIQKRA
                     MQRDFSWKKSAEEYLKVYTDLIRK"
     gene            complement(4437..6416)
                     /locus_tag="PLL88_05950"
     CDS             complement(4437..6416)
                     /locus_tag="PLL88_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ01145.1"
                     /translation="MGKFLILKNAVVLTMDKTLTKYDPGAVAIDDSTITAVGRQDEII
                     ERYPAAELVDCGGKVLMPGFVNTHAHIAMNLVRGFSDDLRLDVWLNGYIMPVEREFVT
                     PDFVRLGTQLGCAELIKTGVTCFLDMYYFEDVVAEATAAIGLRAVLSQTVMKYPTPDA
                     HSYEESLEYCRNFIKKWKGHELITPSVAPHAPYTTGEEILKATKDLALEYDVPLHIHI
                     SETAQEVEVMRKEKGMPVVPFVKKQGVFEAKTIAAHCVHIDEGEMRTLEHSRVGVAHN
                     PSSNLKLASGIAPITRMLELGLKVGIGTDGTASNNDLDFFEEMRLASFLAKGSSGNPT
                     VVPAQQTLEMATRIGAEAIHMADQIGSLEPGKKADLILVELRTLHNSPSFKHSQYGIY
                     AQLVYAGKSTDVTDVMVDGKWLMRNRELLAIDERKLIDQSQVYAEKMDAFIIAREKSV
                     LSKLIAIGGASQEESFEVQAKVVIPDRNKVIEKLETIPFIIKRKRHYREYDTYFKFNA
                     SDEGTVRFREDHFVEADGKVSHVRSRLTLIGPSREHHYPQDVLLSRSRYLAPATQSLR
                     FYREYFKPDSEVEIEKDRLRFLVAFEDEEFFINLDQVNKPDMGSFLEIKSTTWSMRDA
                     EEKSKKIVKLMEALGIAQEEKICKDYLELVEGHLK"
     gene            complement(6423..7553)
                     /locus_tag="PLL88_05955"
     CDS             complement(6423..7553)
                     /locus_tag="PLL88_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOJ01146.1"
                     /translation="MSTAEIITIGTELLLGAITDTNAQYLAKMLNHEGFDVYRITTIG
                     DNCARITQVMAEAMQRSDLVITTGGLGPTVDDPTREAAAQACGAKLVFHEELWQQIED
                     RFRELGRAPTQNNERQAYLPENAVTIRNAVGTAPAFYIKGSHSLLCCLPGVPSEMDFL
                     MKNEVIPLIKRSFKPEALLLTTIVHTIGIGESQIDQLIGHLEKLKNPTVGLAAHAGSV
                     DIRITAKATSHYAAADLISPVLEEVNNLVGQYVFGYDDTTLVSAVCQELVRKGFGQVH
                     FNQQNLGFLNELLTKMMEKVHAGYAENHFKNQYNKSEPRLLTVNVLSRDIKKGKELVV
                     SVEDEKTTTTESYRFFNSPMDFEEWVVNRTMGLVFKHVDQKG"
     gene            7780..8130
                     /locus_tag="PLL88_05960"
     CDS             7780..8130
                     /locus_tag="PLL88_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOJ01147.1"
                     /translation="MQTGRKGEKEAAIFLQKNGVEILGMNYHTPYGEIDIIGKQNDQL
                     IFCEVKTRTSRQFGYPEESITPRKKMALINSSLHYLQTHNQLERVWRIDVVTILKMEG
                     RTQYGWIENAVTGE"
     gene            8096..9043
                     /gene="miaA"
                     /locus_tag="PLL88_05965"
     CDS             8096..9043
                     /gene="miaA"
                     /locus_tag="PLL88_05965"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOJ01148.1"
                     /translation="MVGSRTRLPVNSPVIVILGPSAVGKTALAMKLAKEFSGEIVSAD
                     SRYFYRGMNIGTAKPSAEEMQEIPHYLIDIADPDETISLPQFQAAVYAKIDDILGRNK
                     VPFLVGGTGQYVWGVVEGWLPPRLSPDARLRAVLEDMATHTGAGRIYEMVVMLDPEAA
                     RLIDQKNLRRSIRALEVIFSTGELFSKQKLKDSPPYQFKIIGLQRPRAELYGRVDARI
                     DQMISGGLIEETKKLMEAGYDSSLPSMSAIGYKEIYQYLQHAITLEEAIQSTKRRTRV
                     FIRHQANWFKKDDPRIHWFDMHPGVEKEIATYIRKEGCV"
     gene            9048..10727
                     /locus_tag="PLL88_05970"
     CDS             9048..10727
                     /locus_tag="PLL88_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ01149.1"
                     /translation="MKNFYAQKPWLDHYDPFVSRSLQYPDANIPALFERTVHEFAERV
                     CLSFHGQEITYRELSEYSDNLARWLLFFGVGRGERVGVIFPNSPQFVITFFAVQKIGA
                     VVVAMNPHYKKAEFSRIIEECDIHCVLCMEDHLEILNEILGTTPIEVMISSTNADFKG
                     LNKQIERGVTEGRDVQKIAFPVPFLRCIRQKIQSITVDSSFAADLPAIYQYSGGTTGI
                     PKAAIGLHRNLVANTIQFMSWCDLQVAEEVFLAAIPFYHVYGMVLGMLLGIRVAARIV
                     LIEDARDMDALLSSIMEQRVTFFPGVPSMYATIIRNAAVRAGKYALHSVKACISGSAP
                     LHPQIKEEFEKLTGGKLVEGYGLSEAPTATHCNPVFGDNKPGSFGLPLPDVECKIIDT
                     EDDTRIMEVGEAGELLIKGPQVMAGYFKNAQETTATLRRGWLHTGDIVRMDTDGFFYF
                     IDRKKDVIKVGGFQVWPKEIEEVLLTMRGVRGAAVAGVRSKMGEEKVISWLVLEEDQH
                     ISKAAIRSHCRQFLTAYKIPQEIYFIDEIPRSGVGKVLRRELVRAYNEKRL"
     gene            complement(10751..12610)
                     /gene="ftsH"
                     /locus_tag="PLL88_05975"
     CDS             complement(10751..12610)
                     /gene="ftsH"
                     /locus_tag="PLL88_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOJ01150.1"
                     /translation="MKPSFQTNFVYLLIFIAIFAMIFLNFGQTTNQNTLSINELASRI
                     QAGEIQKITEDENSLTIITRDEQEFTSIKESGSTLVEQLVQYGLTAEDLQSSNVTIEI
                     SQPSAWVDILSIISYLLPFLIIGASFFFIFRQAQGSNNSAMAFGKSRARMFTGDKPKV
                     TFQDVAGVDEAKEELAEVVEFLREPQKFISLGARIPKGVLLVGPPGGGKTLLAKAVSG
                     EAGVPFFSISGSEFVEMFVGVGASRVRDLFEQAKKHSPCIIFIDEIDAVGRHRGAGLG
                     GSHDEREQTLNQMLVEMDGFDTDTNVIVMAATNRPDILDPALMRPGRFDRRVVLDRPD
                     VRGREEILKVHARGKPIAKDIDLSVLAKSTPGFVGADIESLVNEAAILAARHNKKIIE
                     QVDFEEAIERVIAGPQRKSRLISEAEKRIIAYHEAGHAIVISALPEADQVQKISIISR
                     GMAAGYTIALPDDDHMLTSKKKFLADMTGLLGGRAAEEIVFNDITSGAANDIENVTKL
                     ARKMVTRLGMSQELGPMVYGQKDELVFLGREIGEQRDYSDAVAERIDAEVQKLVEIAY
                     QDAKKILLKYKAKLDQVANKLLESETLSREEFEAIFSPPIKKNSGGIPQPNHA"
     gene            complement(12765..13331)
                     /gene="efp"
                     /locus_tag="PLL88_05980"
     CDS             complement(12765..13331)
                     /gene="efp"
                     /locus_tag="PLL88_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOJ01151.1"
                     /translation="MIDVNELRKGVTFQLDNGIYKVLDYHHNKPGRGAATIRVKARNL
                     RTGATIEKTFGSGERVEDIRLEYHMVQFLYPDGNLYYFMDTETFEQPAISKETLGDAV
                     KFLKEGIEVKLTYYGTEPIDVELPTTVDFKVVQSEVAVRGDTATGVSKKVKVETGEEF
                     SVPAFIGTGDVIRIDTRTGEYVTRVQNV"
     gene            complement(13408..13971)
                     /gene="raiA"
                     /locus_tag="PLL88_05985"
     CDS             complement(13408..13971)
                     /gene="raiA"
                     /locus_tag="PLL88_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOJ01152.1"
                     /translation="MSKKVDVFVKNMTLSEDQQKYVNKKAARLDKYLKTIDEIRVDLS
                     YAKTAREMTNRFIAQITLRGKGFILRAEERADEITTAVDKVMDKIERQIERYKGKKYL
                     KRPENLSAVTLSAENPSEQVEEGRPVIARRKTFTLIPMDELEAMEQMTLLGHEDFFIF
                     YNANTNKINVLYKRRDGTYGLIDPVIG"
     gene            complement(13964..14725)
                     /locus_tag="PLL88_05990"
     CDS             complement(13964..14725)
                     /locus_tag="PLL88_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOJ01153.1"
                     /translation="MQIAHHTHTNWLKAYSLAWKILDLIYPPVCIRCGKLGFRCCPDC
                     WATKATYDGNICTICGQPLTHPSLCPDCTAVPPLLEKIRSLGEYVGVLSDLILGLKYH
                     RNIGLAEFVIPDLVRLMAASQFQFDILVPVPLSKTRQRERGYNQVTVWGKLLSATIGT
                     PMLTSALYRHQTTISQVDLPVEKRWENVRGVFSAVEEWVIGKNILLLDDVITTGATLA
                     ECAKVLKEAGANRVSALTIARSSPKKDKNQGGMYV"
     gene            complement(14788..14943)
                     /locus_tag="PLL88_05995"
     CDS             complement(14788..14943)
                     /locus_tag="PLL88_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01154.1"
                     /translation="MKTSSDFEAKGIIEYLLIAVLIFLIIRVLYNLLGPAVKVFIENL
                     LESVATQ"
     gene            15088..15642
                     /locus_tag="PLL88_06000"
     CDS             15088..15642
                     /locus_tag="PLL88_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01155.1"
                     /translation="MTDFYQRVNDEQDFLKKLVAKIPGFSGYVERADRRSADKILRET
                     VAAHFEGLWQQISELQRAFIADGEIELVDDLEASAIKLRQFIDRVKNAGYGYAGFFDA
                     VKVKQEELAAIYEYDLRFLDLESEIASAIENIHASVGTDGLPAAIRNLVALSQNCITA
                     FEKRKEVILSLESENVGDGSSQQE"
     gene            15675..16712
                     /locus_tag="PLL88_06005"
     CDS             15675..16712
                     /locus_tag="PLL88_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOJ01156.1"
                     /translation="MARIFDVVEYPNEMKDEIVHRFPEQGIADLRIGSQVIVRESQEV
                     VFFRDGRALDVLGPGRHTITTANIPLLIDLIGKAFNNRTPFTAEVYYVSKREFADQKW
                     GTPQPIIVRNAGVGLGVALIQGFGTYSFQVKDAQQFVTQVVGAQGIYRMDAIENRLKS
                     MLLSKLQDLLGETTGKKSVLDLIGLTDELSSGVRAKTQDDFNAIGLTLKSFYISSLKP
                     SDKSAEELRAMGMLDMATYTQLQAADALRDAAKNPSGGAGLTAGIGAGMGLGNVISES
                     LKNTGKPNGNASEGPVNVPSVMTPAEAAAVLKVSEEDIVAAINDKSLKARKIGNAYRI
                     SKEALEEFLKG"
     gene            16820..17962
                     /gene="sucC"
                     /locus_tag="PLL88_06010"
     CDS             16820..17962
                     /gene="sucC"
                     /locus_tag="PLL88_06010"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOJ01157.1"
                     /translation="MKIQEYQAKQIFQKYEIPIPRGQVALNASFVRQIAEDLGEKVVV
                     KAQVLVGGRGKAGGIRLADNLDEAEEIATDMMGMEINGLPVHKVLVEEAIDIKKELYL
                     SIGTDRMNASPVIIASEYGGIDIEEAAEETPEKIARILIDPLVGLCDFQMRGLAVSID
                     LPRKHWKKFAQIASALWQIYKNDASLVEINPLVISEQNGLVALDGKMTIDDNAIFRHP
                     ELADLRDLDMENSFEIEAQKCGVSYVHMDGNIGCLVNGAGLAMATMDILKLKGGEPAN
                     FLDIGGGASAEKVSSALNILLSDPQVKAILINIFGGITRCDEIARGITNALSEQKKSK
                     VPVVIRLVGTNAEKGYKILEKQALSIAHSLSEAAETAISLSGVNKE"
     gene            17959..18819
                     /gene="sucD"
                     /locus_tag="PLL88_06015"
     CDS             17959..18819
                     /gene="sucD"
                     /locus_tag="PLL88_06015"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOJ01158.1"
                     /translation="MSILVNRDTRLLVQGITGKEGLFHSELMRAYGTNIVAGVTPGKG
                     GEWVLNEKIPIFDTVQAAVEATEANTSVIFVPAKFAFDSIYEAIDARIPLIICITEGI
                     PAMDMLKIKSVLKTAKSILIGPNSPGVLTPDECKAGIIPGNITFPGNVGVVSRSGTLT
                     YEVLYAMKEVNIGVSTCVGMGGDAITGSGFIDILSMFEQDPGTEKVVLIGEIGGIEEE
                     MAADYIATEMTKPVVAYIAGLSAPKGRRMGHAGAIIEGRAGSAVQKIELFRKAGVKIA
                     EYPEDIPALL"
     gene            complement(18836..19069)
                     /gene="acpP"
                     /locus_tag="PLL88_06020"
     CDS             complement(18836..19069)
                     /gene="acpP"
                     /locus_tag="PLL88_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOJ01159.1"
                     /translation="MSDVLKGVKEVIVDVLKVDEKDISLETSFVEDLKADSMDQFFLI
                     DGFCEKFDLSISDEDARNIKTVKDVVDYIEEKK"
     gene            19151..>19500
                     /locus_tag="PLL88_06025"
     CDS             19151..>19500
                     /locus_tag="PLL88_06025"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the isomerization of isopentenyl
                     pyrophosphate to dimethylallyl diphosphate; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOJ01160.1"
                     /translation="MDNQKIQTRKKDHMRINAEEDVRSSVPTGLERYYFDHQALPEID
                     LEQIDISTTIFMKKQNAPLFISSMVGGTRESAELNTRFARVAQALGIGMGIGSLRIGL
                     QNPEQMKFFDLRSVA"
BASE COUNT         5011 a         4590 c         4859 g         5040 t
ORIGIN      
        1 cgtgggtatt cttattgggc ttgatcccgc aggttatcgg gcacacgttg ttcaacgtgg
       61 cagtgcggca aatggatgca gtatatgcct ccattgcttt gttgggagaa ccgatcggct
      121 ccattattct tgcctggatc tttctcagtg agaagcccgg gcgctacgat ctcttgggcg
      181 gtctctttgt gatcgcggga atcttgctag cctttctgta taagccggtc agcgacaccg
      241 atcatacaac tggttgatgc ccagcagcca attctgcaat ttttttgcat tccacttttt
      301 atccaatttc cactcccagt tattccccaa agaaccaggg taattcattc tggcctcatc
      361 tcccaattcc aggagatctt gcatgggggc gatggcaaaa agggcgactg attgccagag
      421 catgcggatc atggcgtgtg gaacagattc cctgctgtca ccgacatagg cgcggaaatg
      481 ctcgcgggaa gtcggcggga ccgatcggaa ccatcccaag gcggtggaat tatcgtgggt
      541 gccggtgtag gcaacggcat tgcgatcaaa gttgtgggga atgaatcctt tttcagggtt
      601 gggattgaaa ccgaactgca cgatcttcat gcctggcagg tggtatcgtt cgcgcaaggc
      661 gatcacatcg ggcgtcatca cccccaagtc ttcagcaata aagggcaggt cgccaaaatg
      721 cttatagagc gcgtcgaaaa ggtctttccc cggaccttgc acccactgcc cgtgttcggc
      781 ggtcttggaa ccagccggga tctcgtaata ccccgcaaat ccgcggaaat ggtccaggcg
      841 gatgacatct accaggttca aggtctgcct gatccgttca atccaccagg catatccgct
      901 gattttatgc tttttccaat cgtacaatgg attaccccac aattgcccag ttttcgagaa
      961 gaaatctggc ggaacgccag ccacgacggt tggcttgagg tctttgtcga agaagaagag
     1021 ggacggattg gtccaggtat ctgcacagtc gtaaccaacg aagatcggaa tgtctccgat
     1081 gatctgcaca cccttcttgt tggcataggc ttttacttta aaccactggg tgaaaaacag
     1141 gaactgttgg aacttttgct cttcaatctg atccctgttc tctttctgga aatttttcaa
     1201 ccccaacgga tcacggaagc gcaattcctt tgaccattga ttccatgggt gcagttcgtg
     1261 ttgattcttg atggtcataa agagggcaaa atcatccaac caactcttgt gcttgcgaca
     1321 aaagcttttg aactggttga ccaacgcaac gggttggttt tgtttaaaag tcacaaaagc
     1381 ttgctgtaat tttgcgcttt tccagggaat gaccattcca tagtcgacct ttgtgcgggg
     1441 gaagcgcggg cgtttctcga gatcgtgcct ggtaagcaaa cccattgtga cgagatcatc
     1501 cagagcaatg aacaatggat tccctgctgt ggaagaaagc ccctgatagg gcgaattacc
     1561 aaacccggtg ggatttaatg gcagcacctg ccacaatctt gttctggacg ctgcgaggaa
     1621 gtcgataaaa tcgaaagctg gctttcccag atcgccaatt ccatcagcac cgggcaggct
     1681 actgacatgc aacaaaactc cagaagagcg gtcaaatttc atctttgttt cctttcggca
     1741 aaatgacttc tccaaaatga atggtgaggc tgctaaaatc gcgtggcgcc acgtcacatc
     1801 ccaccatgcc acccccttca agctgaacac cttcagggag gatggcattc tttccgacca
     1861 tagatatatg atttttcttt ttcagggctg aaccgatcac gcaatgcttt ccgatgaaaa
     1921 catgcttatc gatgatggct ttctggatgc tggcgtttgg gctgatcatg gtgtcggtta
     1981 gaatgatgga ttcgttaatt ttggttcccc tttgcacaat gaccccggga gagatgacgc
     2041 tgttcttgat ttcaacatca ggttcaatta tacaaccgtc acagatcagc gaagcgttga
     2101 catacgcgcc agggtggata taagcagggg ggcgttcctc agaacgggta tggatgaccc
     2161 aactgcgttt gcgaatatca tagaggggcg gatcgctcaa aaggtccata tgcgctttcc
     2221 aataggtttg gatggttccc acatcgaccc agtaatcacg gtatggaaat gcaaagatac
     2281 gagcatgctc ctgaacgatg gctggcaaca cgtctctccc aaagtcgtgc tgggaggttt
     2341 ggtcctcttc gtcaagaagc agatattttt tcaacacttt tgcattgaaa agatagatgc
     2401 ccatatttgc caggacggag gtgggctttt cgggcttttc cgtgaacttt tgaatgcgca
     2461 tttgatcatc cgtctcgatc acaccaaagc ggctggcttg ctccaacgga acggagtgta
     2521 cgcaaacgct aacctcagca tccatttctt gatgaaagcg cagcaacgct ctgaaatcca
     2581 tagaataaac ctgatcgcca gaaagaacga ggacatattc aggatcgttg ctgagaatgt
     2641 aattcagatt ctgcagaacc gcatcggctg ttccggaaaa ccaattgata cctttcgctt
     2701 tgaaaggagt gaagatcttt attccgcccg taaattctct attcaaatcc catggaccac
     2761 cggatccgat gtggtggatc aaggactgcg ggagatactg agtgatgatg gtcacatcga
     2821 agatactgga attgacacaa ttggataatg gaaaatctat gatgcggtat ttccccgcaa
     2881 atggaacggc tggctttgct cgttttgcag tcaatacacc caagcgggtt ccttctcctc
     2941 ctgcaagaat cactgcacga gttgacattt tttacttcct tatcaagtcg gtataaacct
     3001 tcagatactc ctccgctgat ttcttccaag aaaaatctcg ctgcatggct cttttctgta
     3061 tcttacccca ttctattttg ttgtggtaat gctgcaatgc ttgattcagc gcctccaaga
     3121 aatcagcacg ctgaggatta tgaaacaaga aaccggtacg cgttgtttca ttctcatcaa
     3181 tggaatcgat caacccgcct acagcgtggg cgattggtat gcaaccgtaa tgcatggcga
     3241 tcatctgtga aatgccacac ggttcgtaca gcgagggcat aaggaacata tcaccacctg
     3301 cgggcaagag atgagaaaaa gcggcatcaa aacgattcag gaaccgaata gtttgcgggt
     3361 gagcctgttc gagagcgtgc acgagttctt cctggtcgtg gttgcctgtt cccaggatga
     3421 ccgcctgcca cttttcactt ttaaagttac gcaaggtatc gcagagcagg cgaatccctt
     3481 tttgacgatc caggcggctg accattacta gcagaggcac atccggttct accgcaagcc
     3541 ccagcgcatt ttggataaaa accttatttt ctttgcgggc ttccaactgg tgcgcattga
     3601 aattcttctg aataaaacaa tcggtttgcg gatcccagaa tttttcatcg ataccattga
     3661 ggataccgct gatcttttgc tttctggtag aaaggtactc ttgcaggtca tttccgaatt
     3721 gcggcgtcaa tagctcctgc gcataacgag gggagacggt gatgacccga tcggcgtgtt
     3781 cgatgccaat caccaatgga agatcctttc cccacgctgg aacggcattg gtgtgcgcag
     3841 caggaacctc atacttctgg atgaacggtt ctgcgcccgc tcccatataa ggaaggttgt
     3901 gaatgacaaa tatactcttg gtccttttcc acgccgcatc ctggctgtgt ttcactgcgc
     3961 tgcaaactgc cgcggtttgc cagtcgttgg cgtggatgat atccgctttc caatccaacg
     4021 cttccatcaa gtcgaggcag gcaagagaaa atgcgatgaa cttctcacca tcttcggcag
     4081 gattggtggt gtacacgata tcctgctgat cctccaaacc tgtattatca aggacataat
     4141 agatgagcga gtcaagcgtg gtggtatata cctggtaatg gtttatcttt ctggcatagc
     4201 ggctggtgaa tgagcacgac gccgttttat ccagaaatcg tgaatccatc atctttgtgt
     4261 aaggaacagc aatgcggata tcgaaagaga catccgcatt gttattttgg gcggtttcga
     4321 gcagcgcttt gggcaaagag ccaacatagt ctcccagacc gcctatcttg accaaaggcg
     4381 ccgcttcagc ggccatgaat agtaccttta atcttccctg agaatccgct ggcatgttat
     4441 ttcaggtgtc cctcgaccaa ctccaaatag tccttacaga ttttttcttc ctgagcaatc
     4501 cctagcgctt ccatcaattt cacgatcttt tttgattttt cttccgcgtc gcgcatactc
     4561 caggtggtgg acttgatttc aagaaaactg cccatatccg gtttattgac ctggtcaagg
     4621 ttgataaaga attcttcatc ttcaaatgca accagaaaac gcaagcgatc cttttcgatt
     4681 tccacctcag aatctggttt gaagtactcg cggtaaaagc gcagactttg ggtggcagga
     4741 gccagatagc ggctgcgaga aagcaaaaca tcttgtggat agtgatgctc tcgcgaagga
     4801 ccgatgagcg tcaatcgaga gcgcacgtgc gaaaccttgc catcggcttc aacaaaatgg
     4861 tcttctctga agcgcacggt gccttcgtca ctggcattga acttgaagta ggtgtcgtat
     4921 tcgcggtagt gccgtttgcg tttgatgatg aaggggatgg tttcgagttt ttcgatcacc
     4981 ttattccgat cgggaatgac cactttcgcc tgaacctcaa aactttcttc ctggctggca
     5041 ccaccgatcg cgatcaattt ggaaagaacc gacttttctc tggcaatgat gaacgcgtcc
     5101 attttctcag catatacctg gctttgatcg atcaatttcc tctcatcgat agccagcagt
     5161 tctcgattgc gcatcaacca tttgccatca accatcacat cggtgacatc ggttgatttg
     5221 ccagcataaa ccaactgcgc atagattcca tattggctat gcttgaagct gggactgtta
     5281 tgcagcgttc tcagttcgac caagataagg tccgctttct tgccaggttc caggctgccg
     5341 atttggtcag ccatgtgaat ggcttcagct ccgatgcggg tagccatttc taacgtttgc
     5401 tgggcaggca ccacggtagg attcccactg gaacctttgg caaggaagga tgccaggcgc
     5461 atctcttcaa aaaagtcgag atcattgttg gaggctgtcc catcggtgcc aatgcccacc
     5521 ttcaaaccca attccagcat gcgggtaatg ggagctatgc ctgatgccag tttaagattg
     5581 gaagaggggt tgtgcgctac ccctacgcgg gaatgttcca acgtgcgcat ttcgccttca
     5641 tcgatgtgca cgcagtgagc agcaatggtt ttggcttcaa agacgccctg tttcttgaca
     5701 aagggaacta ccggcatgcc tttctctttc cgcatgactt ccacttcttg ggcggtctcg
     5761 gagatatgga tatggagggg aacatcatat tccaatgcca gatctttggt agctttgagt
     5821 atttcttcgc cagtcgtata aggcgcgtgc ggggcaacag aaggggtgat gagttcgtga
     5881 cctttccatt ttttaatgaa gttgcggcaa tattccagcg actcttcgta cgaatgggca
     5941 tctggagtgg ggtatttcat cacggtttgg gagagcaccg ctcgcagccc gatggcagcg
     6001 gtggcttcag ccaccacatc ttcaaaataa tacatatcca aaaaacaggt gacaccagtt
     6061 tttatcaatt cagcgcagcc aagctgggtg cccaaccgca caaaatctgg agtgacaaat
     6121 tcccgctcga ccggcatgat atatccattc aaccaaacgt ccaggcgcaa gtcgtcggaa
     6181 aagccgcgca caaggttcat ggcgatgtgc gcatgcgtat tcacgaatcc gggcatgagc
     6241 acttttcccc cgcagtcgac cagctcggca gcgggatatc gttcgatgat ctcatcttgc
     6301 ctgccaaccg cagtaatcgt cgagtcatcg atcgccactg cacccgggtc atattttgtg
     6361 agggttttgt ccattgtcag gacaacggca ttcttcaaaa tcaggaactt tcccattttc
     6421 acctatcctt tttgatctac gtgcttaaaa accagcccca tggtcctgtt gacaacccat
     6481 tcctcaaaat ccattgggct attaaaaaac cgatagcttt ctgttgttgt ggttttttca
     6541 tcttcaacac ttactaccaa ttcttttccc tttttgatat ctcttgacaa gacattgacg
     6601 gtgaggagcc gcggttcact cttattgtac tgatttttga aatgattttc tgcatagccg
     6661 gcgtgaacct tttccatcat tttcgtcaag agttcgttca aaaatcccag attctgttga
     6721 ttgaaatgca cctgtccaaa acctttgcga accagctcct ggcacaccgc gctcacaagg
     6781 gtcgtatcgt catacccaaa aacgtattga ccgaccaagt tgttgacctc ttccaaaacc
     6841 gggctgatca ggtcagccgc agcatagtgg ctggtagctt ttgcggtgat acgaatatcg
     6901 acactgcccg catgggcagc aagaccgacg gttgggtttt tcaatttttc caaatgcccg
     6961 atcaactggt cgatttgtga ttcaccgatg ccaatggtgt gcacgatggt ggtgagcaag
     7021 agcgcctcgg gtttgaacga acgtttgatg agaggaatga cttcattttt catcaaaaaa
     7081 tccatttcag aaggaacacc gggcaaacag cacagaagcg aatgtgaacc tttgatgtag
     7141 aacgctggcg ccgtgccgac agcattcctg atggtaacgg cattttcagg caaataggct
     7201 tgccgttcat tgttttgagt aggggcgcga cccagttcac ggaaccgatc ttcaatctgt
     7261 tgccacagct cttcatgaaa aacaagcttc gcaccgcatg cctgtgctgc agcttcgcgg
     7321 gtaggatcat ccacggttgg acccaagcct ccggttgtga tcaccagatc actacgttgc
     7381 atggcttccg ccatcacctg cgtaatgcgg gcgcagttat caccaatcgt ggtgatgcgg
     7441 tacacatcga aaccttcatg gttcagcatc tttgccaaat actgtgcatt ggtatctgta
     7501 atggcaccca acaacaattc cgtgccaatg gtgatgattt ctgcggtaga catcgagttt
     7561 tccttaaaat ttcaccaatt ttagcagaac tttccctgga gagatatagg aatattcagc
     7621 gtgatcattg cggaagaaac ctgcttccag gtgaagccat tttcggttcc gaccagcgga
     7681 gagtcgagat atgcataacc aggacatggt ttcattgttc cttttttaat ctcccatttg
     7741 gaaaaattcc attatgataa taacgtgaca aagcaaagca tgcaaacagg tagaaaaggt
     7801 gaaaaagaag ctgccatttt tttgcagaaa aatggcgtgg aaattctggg tatgaactat
     7861 catacaccct acggagagat cgatatcata ggcaaacaga acgaccaatt gattttctgt
     7921 gaagtaaaaa cgcgcacatc cagacaattc ggctatcctg aagaaagcat cacaccgaga
     7981 aagaaaatgg ctttgatcaa ttccagtttg cactatttac agactcataa ccaactggag
     8041 cgtgtgtgga ggatcgatgt ggtgacgatt ttgaagatgg agggacggac acaatatggt
     8101 tggatcgaga acgcggttac cggtgaatag ccccgttatt gtcatcctcg gtcccagcgc
     8161 ggtgggcaag accgcacttg cgatgaagct ggcaaaggag ttttcgggtg aaattgtttc
     8221 agccgactcc cgttattttt atcgggggat gaatatcggc acagcaaagc ccagcgcaga
     8281 agaaatgcag gaaattccgc actatcttat tgatattgcc gacccagatg agaccatcag
     8341 cttgccccaa ttccaggcgg cagtatatgc gaagatcgat gatatcttag gacggaacaa
     8401 agttcccttt ctggtaggcg gaacggggca atatgtgtgg ggggtggtgg aggggtggct
     8461 gccgcctcgc ttaagccctg acgctcgcct gagagcggta ttggaagaca tggcaacgca
     8521 cacgggcgcc gggcggatct atgagatggt ggtaatgctt gaccccgaag ctgccaggct
     8581 gatcgatcag aaaaatctca ggcgctccat ccgcgcgctt gaggtcattt tttctacggg
     8641 agaattattc tcgaaacaga agttgaaaga ctccccacca taccaattca aaataatcgg
     8701 tttacaacga ccgcgagctg aattatatgg gcgcgtggat gcgcgcattg accagatgat
     8761 aagcggcggg ctgatcgagg aaaccaaaaa gttaatggag gcggggtatg attcaagctt
     8821 gccttccatg tctgcaatcg gatacaagga aatttaccag tatctgcagc atgcaatcac
     8881 cttagaagaa gcgatccaat cgactaagag gcgcacccgt gtctttatcc gccatcaggc
     8941 gaactggttc aaaaaagatg atccgcgcat tcattggttc gacatgcacc caggtgtgga
     9001 gaaagaaatc gcgacttata ttcggaagga aggttgtgta taagtaaatg aagaatttct
     9061 acgctcaaaa accatggctt gatcattacg atccgttcgt atcccgctcg ttacaatatc
     9121 cagatgcgaa cattccggct cttttcgagc gaactgttca cgaatttgcg gaacgggttt
     9181 gcctttcatt tcatgggcaa gagataacgt atcgggagct atctgaatac tccgacaatc
     9241 tcgcccgttg gctactcttt ttcggcgtcg gaagagggga gcgggttgga gtgatcttcc
     9301 cgaactctcc tcaatttgtc atcacattct ttgctgttca aaaaatcggc gccgtagtgg
     9361 ttgccatgaa cccccattac aaaaaagcag agttttcccg catcatcgaa gaatgcgaca
     9421 tacactgtgt gctttgcatg gaggaccatc ttgaaatatt gaatgaaatt ttaggaacaa
     9481 ctccaatcga ggtcatgatc agttcgacaa atgcggattt taaagggttg aacaagcaga
     9541 tcgaacgcgg cgtaacagaa ggcagagacg ttcaaaagat agcgtttcct gttcctttct
     9601 tgcgctgcat acgacaaaaa attcagagta tcacggtgga ttcctccttc gctgcggacc
     9661 ttcccgcaat ttatcagtat tccggcggga cgaccggcat tcccaaagcc gctatcgggt
     9721 tgcacaggaa cctggtcgca aacacgatcc aattcatgag ctggtgtgac ctgcaggtgg
     9781 ctgaagaggt ctttttggca gccattccct tctatcacgt gtatggcatg gtgctgggca
     9841 tgctgttggg cattcgcgtg gcggctcgca tcgttctgat cgaagacgcg cgggatatgg
     9901 atgcgctgtt aagttccatc atggagcaac gggtcacatt tttccctggc gtacccagta
     9961 tgtatgccac catcattcgc aatgcggcag tccgcgcagg aaagtatgcc ttgcactccg
    10021 tgaaggcatg catttctggc tcagccccgc tgcaccccca aataaaagag gagttcgaaa
    10081 agttgaccgg gggaaaattg gtagaagggt acggtctgtc tgaagcgccc accgctaccc
    10141 actgcaatcc ggtgttcgga gacaataaac caggttcttt tgggttgcca ctgccggatg
    10201 tggagtgcaa aattatcgat acggaggatg atacgcggat catggaggtg ggagaagcgg
    10261 gtgaactgct cattaaggga ccgcaagtta tggcaggata tttcaagaat gcacaggaaa
    10321 ccacggcaac attacgcagg ggttggctgc acacagggga tatcgtgcgc atggataccg
    10381 atggtttttt ctatttcatc gaccgaaaaa aagacgtgat caaggttggt ggttttcagg
    10441 tttggccgaa ggagatcgaa gaggtgctat tgaccatgcg gggggttcgt ggggctgctg
    10501 tggctggagt gcggagcaag atgggagagg agaaagtgat ctcgtggctg gtgttggagg
    10561 aagatcagca catcagcaag gcggctatcc gcagccattg ccggcaattc cttacggcgt
    10621 ataaaattcc gcaagagatc tatttcatcg acgagatccc acgatcgggg gtagggaagg
    10681 tgttgagacg ggagctggta agagcctata acgaaaaacg gctttgagat cctcaaaacc
    10741 gtttttactc ctatgcatgg ttgggttggg gaataccacc gctgttcttt ttaatcggcg
    10801 gggagaagat cgcttcgaat tcctcccttg aaagggtttc cgattcaagg agtttgttgg
    10861 caacctgatc caacttggct ttgtatttca gcaagatctt cttggcatct tggtaggcga
    10921 tttcgaccaa cttttgaact tcagcatcga tcctttcggc gactgcgtcg gagtaatcgc
    10981 gctgttcgcc gatctcacga ccgaggaaca ccaattcgtc tttctgacca tacaccatcg
    11041 gtccaagttc ttgactcatg ccaagcctgg tgaccatctt gcgcgccagt ttcgtcacgt
    11101 tctcaatgtc gttcgcagcg ccggatgtga tgtcattgaa gacgatctct tcagccgcac
    11161 gtccacccag caaaccggtc atatcagcga ggaacttctt cttcgaagtg agcatatggt
    11221 catcatcggg cagggcaatg gtgtagccgg ctgccattcc acgcgaaatg atggaaatct
    11281 tttggacctg atctgcttca ggaagcgctg agatcacaat ggcatgcccg gcttcgtggt
    11341 aagcgatgat gcgtttttcc gcttcgctga tcagccggct cttgcgctgc ggaccagcga
    11401 tgaccctttc gatcgcttct tcaaaatcaa cctgttctat gatcttcttg ttgtggcgcg
    11461 cagccaaaat tgcggcttcg tttaccaggc tttcaatatc ggctccgaca aagccaggtg
    11521 tgcttttggc aagcaccgac agatcgatgt cctttgcgat cggttttccg cgcgcatgga
    11581 ccttgaggat ctcttcacgc ccgcgcacat ctgggcgatc taacacaact cgacggtcaa
    11641 aacgaccagg gcgcatcaaa gccggatcaa gaatatccgg acggttggtg gctgccatta
    11701 caatgacgtt ggtatcagta tcgaaaccat ccatttcaac caacatctgg ttgagggtct
    11761 gctctcgttc atcgtgactc ccgcccaatc cggcgccgcg gtggcgccca acagcatcga
    11821 tttcatcgat gaaaatgatg cagggggagt gcttcttggc ttgctcaaac aggtcgcgca
    11881 cccggctggc gccaacaccc acgaacatct ccacgaactc tgaaccggaa atggagaaga
    11941 atggcacgcc cgcctcaccg gagactgctt tagccagcaa ggtttttcca cctccgggtg
    12001 gaccaaccag cagcacgcct ttgggaattc gagcaccgag ggaaatgaat ttctgcggtt
    12061 cgcgcaagaa ttcaaccact tctgccaatt cttctttggc ttcatccacg ccagctacgt
    12121 cttgaaatgt gactttgggt ttatcaccgg taaacatgcg cgcccgggat ttcccaaatg
    12181 ccatggcgga attattactg ccttgcgctt gccgaaaaat gaagaagaaa gaagcgccga
    12241 ttatgagaaa aggcaagagg tagctaatga tagagaggat atcaacccac gcactgggct
    12301 ggctgatttc gatggtcacg ttggaggatt gcaaatcttc agcggttagc ccatattgga
    12361 ccaactgttc caccagggtg gatccgcttt cttttatcga ggtaaattct tgttcatctc
    12421 tggtgataat ggtcaggctg ttttcatctt ctgtaatctt ttgaatctcc cctgcttgga
    12481 tcctggaagc caactcattg atcgagagcg tattttgatt ggtggtttga ccaaaattca
    12541 gaaaaatcat tgcaaagatc gcaatgaata tcagcaaata gacaaagttc gtttgaaaac
    12601 taggtttcac ataaacctcc agctcatgtt agttcgaacg tgattatacc aatcattcat
    12661 taacataacc taaatatatg gaagcaaaga attattaaga gatttcatat aaaaaagaat
    12721 cccgcatttt atggcgggat tcttttttcg tttttgagct tctgttatac gttttgaacg
    12781 cgggtgacat actcaccagt gcgcgtatcg atgcgaatta catcaccagt gccaatgaac
    12841 gctgggacgc tgaactcttc accggtttcc actttgactt tcttactcac gccggttgct
    12901 gtatcaccac ggacagccac ttcggactgg acaactttga aatcgaccgt ggttggcaat
    12961 tcaacatcaa tcggttctgt gccgtagtag gtgagtttca cctcaatgcc ctctttcagg
    13021 aacttcacgg cgtcgcccaa cgtttctttt gaaatagcag gctgctcaaa ggtttccgta
    13081 tccatgaaat aatacaggtt gccatcggga tagaggaatt gaaccatatg gtattccaga
    13141 cgaatatctt ctaccctttc tccagaacca aaggtctttt cgatggtggc acccgtgcga
    13201 agatttctgg cttttacacg gatcgtggct gcaccgcgac ctggtttatt atggtggtaa
    13261 tcgagcacct tgtagatgcc attatcaagt tggaatgtta cgccttttcg cagttcattt
    13321 acatcaatca tagctacacc ttctcaggga tattcaaaaa accatgagat tatagcatgc
    13381 aaatgcaatt tccttgaagg gtctgaatca tccaattacg ggatcgatca gaccataggt
    13441 gccatcgcgg cgtttgtaca agacgttgat cttattggtg ttcgcattgt agaaaatgaa
    13501 gaaatcttcg tgtccgagca aggtcatctg ctccatcgct tccagttcgt ccatggggat
    13561 gagggtgaag gttttgcggc gagcaatcac cggacgacct tcttcgacct gctcagaagg
    13621 gttttctgct gaaagggtta cagcactcaa gttttcagga cgtttgagat attttttccc
    13681 tttatatcgt tcgatctgcc gttcgatctt atccataacc ttatcgacgg cagtggtgat
    13741 ctcatcagcg cgttcttctg cacgaaggat gaacccttta ccgcgcaagg tgatttgagc
    13801 aataaaacgg ttggtcattt ctcttgctgt tttggcatag gaaagatcta cccgaatttc
    13861 atcaattgtt ttgaggtatt tatcgagcct ggctgctttt ttgttaacat atttctgctg
    13921 gtcctcagat agggtcatat tctttacaaa aacgtcaact ttcttagaca tacatacctc
    13981 cttgattttt atctttcttt ggtgatgatc ttgcaatggt taaagcggat acacgatttg
    14041 cccctgcttc tttcagtacc ttcgcgcatt cagccaatgt cgcgccggtg gttatcacat
    14101 cgtccaatag taaaatgttt ttcccgatga cccattcttc aaccgccgaa aaaaccccac
    14161 gcacattttc ccaccgtttt tctaccggca ggtcaacctg ggatatggtg gtctggtggc
    14221 gatagagagc cgaagtgagc attggggttc caatggttgc gctcagcagt tttccccaca
    14281 cagtaacctg gttatatccc cgctcgcgct ggcgggtttt actcaaggga accggaacga
    14341 ggatatcaaa ttggaattgc gatgcagcca tgagtcgcac caggtcggga atgacgaact
    14401 cggcaaggcc aatattgcga tggtatttca agccaaggat taaatcgctg agcactccaa
    14461 catattcccc caaagaacgg atcttttcca ggagaggggg gacggcggtg cagtcgggac
    14521 aaagggaagg gtgggttagg ggttgcccac aaatggtgca aatgttacca tcatacgtgg
    14581 cttttgttgc ccaacagtcg gggcaacagc gaaaaccgag ctttccacaa cggatacata
    14641 cggggggata gatgagatca aggattttcc aggcaagaga atatgctttt agccaattgg
    14701 tatgtgtatg gtgggctatc tgcatctcgc tttcttccgt cgatccagat tcttctacac
    14761 atcaatcata caacatttta aaaatttcta ctgggttgcc accgattcta ataaattttc
    14821 gataaatacc ttcacggcgg gaccgagcaa gttatataga acacgaatga tcaagaaaat
    14881 cagcactgca ataagaaggt attcaatgat ccccttggct tcaaaatcag atgatgtttt
    14941 cataccacat tctctgtttt cagtataccg ttattttctt cgagccgcaa ctttttctta
    15001 tagaatgcgt attgtaaaag gaaatatgta tatataataa agatgaattc aaacatgaaa
    15061 tgaatgatca agttcgtgga ggataaaatg actgattttt accagcgtgt gaatgatgaa
    15121 caagactttt taaagaaact tgttgcaaaa atccccggtt ttagtgggta tgttgaacgt
    15181 gccgaccgga ggtctgccga taagatcctc cgcgagacag ttgcggctca ttttgagggt
    15241 ttatggcagc agatttcaga actgcaacga gcattcatcg ccgacggcga gatcgagttg
    15301 gtagacgatc ttgaagcatc agccatcaaa ctgcgccaat tcatcgatcg cgtgaaaaac
    15361 gcgggctatg gctatgcagg tttctttgat gccgtgaagg taaagcagga agaattagca
    15421 gccatctacg aatacgacct gcgttttctg gatctggaaa gcgagatagc cagcgcaatc
    15481 gagaatatcc acgcttctgt cggcactgac ggactgcccg cagccatccg aaatctggtt
    15541 gccctatcgc aaaactgcat caccgctttc gaaaaacgga aagaagtgat tctctcgctg
    15601 gaaagcgaga acgtgggtga tggttcatca caacaggaat gaaataatct atctaatgca
    15661 tatggagaaa agaaatggct agaatttttg atgtagttga atatcctaat gagatgaagg
    15721 atgagatcgt tcatcgtttt ccagagcaag gaattgctga cctgcgcatt gggtcacagg
    15781 tgatcgtaag agaatctcaa gaagtggtct ttttcagaga tggtagggcg ctggatgtgc
    15841 tgggacccgg acgacacacc atcacaactg ccaatatccc gttgctgatt gacctcatcg
    15901 gcaaagcgtt caacaaccgc acacctttta ccgccgaagt ttattatgtt tccaagagag
    15961 aattcgcaga tcagaaatgg ggaaccccac aaccgatcat cgtacggaat gcgggcgttg
    16021 gattgggtgt tgctctcatc caggggtttg gaacgtacag tttccaggtc aaggatgccc
    16081 agcagttcgt gacgcaggtg gtcggcgccc agggcatcta tcgtatggat gccatcgaga
    16141 accgcttgaa atccatgctg ctttctaaac tgcaggatct gcttggcgaa acaaccggga
    16201 agaaatcagt gcttgacctc atcggtctga ccgacgaatt gagctccgga gtgcgtgcca
    16261 aaacgcaaga tgacttcaac gcaatcggct tgacgctgaa atctttctac atctccagct
    16321 tgaaaccctc agacaaatct gcagaagaac tgcgcgccat gggtatgctg gatatggcaa
    16381 cctatacaca attgcaggct gcagatgcac tacgcgatgc ggctaaaaac ccaagcggcg
    16441 gtgcgggttt gaccgctggc attggcgccg gtatgggctt aggaaatgtc atctctgaat
    16501 ccttgaagaa cactggaaaa cccaacggaa atgcgagtga gggacctgta aacgtcccat
    16561 cggtgatgac acctgctgaa gcagcagcgg tcttgaaggt gtctgaagag gatattgtgg
    16621 cagccatcaa tgataaatcg ctcaaagcga ggaagattgg caatgcttac cgcatcagca
    16681 aagaggcgtt ggaagaattc ttgaaaggtt aaaaagtatc tagttactgc aagcccggtg
    16741 attttcatcg ggctttttct ttttatccgc tatgttataa tcgcttgcgg tgggcaacaa
    16801 ttttatataa ttggatgcaa tgaaaataca agaatatcaa gccaaacaga tatttcaaaa
    16861 gtacgaaatt ccaattcccc gcggacaagt agctcttaat gcaagttttg tcagacagat
    16921 cgctgaagat ctgggtgaaa aagttgtagt taaagcgcag gttctggtgg gcggacgtgg
    16981 aaaagccggg ggaattcgat tagcggataa tctggatgag gcagaagaga tcgcgaccga
    17041 catgatgggg atggaaatca atggattacc agtccataag gtgttggttg aagaagcgat
    17101 cgatatcaaa aaggagttat acctgagcat cggaactgat cggatgaacg cttctccggt
    17161 gatcatcgct tcagaatatg gcgggatcga tatagaggag gctgcagaag aaacgcctga
    17221 aaagatcgca cggattctca ttgaccccct ggttgggttg tgcgatttcc agatgcgtgg
    17281 tctggcggtg tccattgacc tgccacgaaa gcactggaaa aaattcgcgc aaatagcaag
    17341 cgctttatgg cagatctaca aaaatgacgc ctcactggtg gagatcaatc cgctggtcat
    17401 ctcggaacaa aacggtttgg tggctctgga tgggaaaatg accatcgatg ataatgccat
    17461 cttccgccat ccagagctgg cagatctgcg cgatctggac atggaaaact cctttgagat
    17521 cgaagcacag aaatgtggcg tctcctatgt tcacatggat gggaatatag gctgtctggt
    17581 caacggagcg ggtctggcaa tggcgaccat ggatattttg aaattgaaag gcggggaacc
    17641 ggctaatttt ctggatatcg gtgggggagc cagcgcagaa aaagtatcct cagcactcaa
    17701 catcttgctt tcagaccctc aggtgaaggc gatcttgatc aatatctttg gagggataac
    17761 ccgttgtgat gaaattgcac gcggcatcac caacgcgctt tcggagcaaa agaaaagcaa
    17821 agttccggtg gtgatccggc tggtaggaac caatgccgaa aaggggtata agatactgga
    17881 aaaacaggct ctgtccattg cacattcgct ttcagaggct gctgaaacgg caatctctct
    17941 ttcgggagta aataaggaat gagcattctc gtcaatcgcg acacacggtt gctggttcaa
    18001 ggcatcaccg ggaaagaagg tctgttccac agtgaactga tgcgagcata tgggacgaat
    18061 atcgtggcag gtgtgacgcc cggcaagggt ggggaatggg tattgaatga gaagatacca
    18121 atttttgata cggttcaggc ggcggtcgag gcgaccgaag cgaacacgag cgtgatcttt
    18181 gtgcccgcta aatttgcgtt cgattccatt tacgaggcaa tcgatgcaag gatccctttg
    18241 atcatctgta tcaccgaggg cattcccgcc atggatatgc ttaaaatcaa atcggtcttg
    18301 aagactgcaa agtcgatctt gattggacca aactcacccg gggttttgac ccctgacgag
    18361 tgtaaagctg gcatcattcc cggaaacatc acctttccag gaaacgtcgg ggtggtatcc
    18421 cgttccggca cattaaccta tgaagtgttg tacgctatga aagaggtcaa tattggcgtt
    18481 tccacctgtg tggggatggg gggagatgcc ataaccggat caggtttcat cgacatactt
    18541 tccatgttcg agcaagatcc tggcacggag aaggtggtgt taatagggga aattggggga
    18601 atagaagagg agatggcagc cgattacatt gctacagaaa tgaccaagcc ggtggtggct
    18661 tatattgcag gactttccgc tcccaagggc agacgcatgg gacatgcagg tgcgatcatt
    18721 gaaggaagag caggttctgc cgtgcaaaaa atcgagttgt tcaggaaagc tggtgtaaaa
    18781 attgcagaat atccagaaga tattcctgct cttctctaga cccgctcaat gcgggttact
    18841 ttttctcttc aatataatcg acaacgtctt tcactgtttt gatgtttctg gcgtcttcat
    18901 cggaaatgga aaggtcgaat ttttcacaaa acccatcgat caggaagaac tgatccatgg
    18961 agtcggcttt caaatcttca acaaagctgg tttcaagcga gatgtctttt tcgtcgactt
    19021 tcaaaacatc cacgatcact tcttttacgc ctttcagtac atcactcatt gagatacctc
    19081 caaatgctgt tttaaatgta acatcaatta tagtattaaa cacaaaaaaa caagaagtgt
    19141 tgcagagtac atggacaatc aaaagatcca aacgcgaaag aaagatcaca tgcgcatcaa
    19201 cgctgaagaa gatgtgcggt cttccgtccc tacggggttg gaaaggtatt atttcgatca
    19261 tcaagcattg cctgagatcg accttgagca gattgacatt tccacaacga tcttcatgaa
    19321 aaagcagaac gcccccctct ttatttcctc catggtgggt ggcacccggg aatcagcaga
    19381 gttgaacacc cgctttgccc gggtggcaca ggcattaggg atcggaatgg gcatcggttc
    19441 gctgcggatt gggttgcaaa accccgagca aatgaaattc ttcgacctgc gatccgtcgc
//
LOCUS       DAOSDS010000038        19168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1189_length_19168_cov_4.882227, whole genome shotgun sequence.
ACCESSION   DAOSDS010000038 DAOSDS010000000
VERSION     DAOSDS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 19168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19168
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1189_length_19168_cov_4.882227"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="PLL88_06030"
     CDS             complement(<1..779)
                     /locus_tag="PLL88_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOJ01161.1"
                     /translation="MENEFNSVIDTISVRQQTFQFYSLPKLQEQGFGTIEKLPYCMRI
                     LLEGMLRNCGKKGFTRQHILTFLQSEHTDQDTVLLLPNRIILQDFTGIPVLNDLAALR
                     AAAAKRNIDPRSINPVIRTDLVVDHSLQVDHTGCERAQELNEELEYRRNRERYEFLRW
                     CEQAFQNLHIIPPGNGIIHQINLEYLAEVASIRQSEGNLTIIPDCVLGTDSHTPMVNG
                     LGVVGWGVGGIEALAAMLGYPMEFTFPRVVGLHLNGCLPEN"
     gene            complement(795..2039)
                     /locus_tag="PLL88_06035"
     CDS             complement(795..2039)
                     /locus_tag="PLL88_06035"
                     /inference="COORDINATES: protein motif:HMM:NF005425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOJ01162.1"
                     /translation="MAQDKAEHITIPYIIGDGIGSDIWNATRRVLERAIESAYSGTRA
                     VDWLEIAAGEKAFRESGTYLPPESIEQLKKHKVGIKGPLTTPVGGGYTSLNVTLRKEL
                     DLYSCVRPVRWFPGLPSPLSHPENVNVIVFRENTEDLYAGIEFPAETEENSIILDLLK
                     TRFPNDFAKLRYAQEVGIDLKPISKQASQRIMRAAIQYAIKNKRHKISIVHKGNILKY
                     TEGAFRNWAYEIAESEFSDVCYSKRAWKRTFDKKGKGKADSEKAEALRRGMIFVDDLI
                     ADNAFAKAIVSPADFDVVVTTNLNGDYISDAFAALVGGIGVSPGANINSELGWGLFEA
                     NHGSAEDIAGMDKANPSSLILSAAMLFDFLGWHEVAALLRDGIEKTIQAGHVTFDLHT
                     QLPGSTLVSTSQFSALVMKNMA"
     gene            2168..2935
                     /locus_tag="PLL88_06040"
     CDS             2168..2935
                     /locus_tag="PLL88_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOJ01163.1"
                     /translation="MTTEKVQILQRVIDILDCFTLERPSLGVREVARMVNLSSSTTGR
                     LMVAMKDLRILNQDPVNKTYSIGSRVLTWAGIYTATSDLRAVALPVMVNLLERTRETI
                     SLYVLEGKERVCIERLESPEPVRITARVGRRIPLYAGSAGKCMLAFMPEEKCEEILNQ
                     IVLTPLTQATIRDKDALRRDLANIREEGCAVSYGEWILEASGVAAPIFNEFGTLVAAL
                     TISGPGQRFVEEKIQDYKELVKEKAREISAKLGYMPR"
     gene            2960..4303
                     /locus_tag="PLL88_06045"
     CDS             2960..4303
                     /locus_tag="PLL88_06045"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF007128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HOJ01164.1"
                     /translation="MELAKKLEGKIREVYEQVRYMLAVSGETIISQVSLQQFFSGNRG
                     VDLLMSDVSFVDPFDGLHYRGIPIDQVLNELPKPDGNAFPYAGGLYAFLLTGSFPTLA
                     EALEVEDVWRARASIPAYVVEILKAMPSDSHPMTMFSQAILALQGQSKFAKTCSDGLR
                     REDHWKVMLEDSLDLTAKTPALAAAIYNIKYGNGSPREIDPALDWSANFAHLIYKEWD
                     VQYQDLCRLFFCLHADQGVGNVSAHAACLVNSALSDVYYSCSAAMNGLAGPLHGKANQ
                     GCLEWLLKIREKFNNALPTMDELESFVWDTLQEGKVIPGYGHAILRCTDPRFTAQYEF
                     GKQYLPDDDLFKLVDMLYQVVPAALKKQGKAKGIWPNVDAISGILQYHCGLKQFDFYT
                     VLFGVGRMLGITANLVWNRALMLPLERPQSITLEMVKNRLHRNLDFPAMNHDFRY"
     gene            4367..5482
                     /locus_tag="PLL88_06050"
     CDS             4367..5482
                     /locus_tag="PLL88_06050"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOJ01165.1"
                     /translation="MQVQCEQCGRKEDYRPAEWRCACGGAWILRASQPLVPELIAKDT
                     NSLWRYSAFFPPEIQKMRNTLAAGFTPLLPCRLQDRDVLLKMDYLNPTASFKDRGVEL
                     MMNAMAAAGIERVVEDSSGNAGASVAAYSACLGMKASIFAPALASPMKLAQISLYGAE
                     VRLIPGARQNASHAALEELEKGAIYASHAYNPVYLLGQQTAAWEVWEQSGYHAPDWVA
                     VPVGQGGNLLGYYEGFRALLVAGLIEKIPGIIAVQPERLAPLCDAYHEGWEEWHTVPP
                     PQDGSVAEGLAITQPVRWKQIIAAIKESHGFCVKVAEEDILPAQRRLAGKGFYVEPSS
                     AVIVAALNEIYENVERKQTIVLSLTGSGLKSGPLTSQ"
     gene            5505..6578
                     /locus_tag="PLL88_06055"
     CDS             5505..6578
                     /locus_tag="PLL88_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOJ01166.1"
                     /translation="MNPIFFTRGVPPRESIPSSVLAECAKTAIAEEGADILQYSSSAG
                     YPPLREVIARAKGISMERVILGQGSLQILDILLHCLLYPGDAVAVEQPTYDRVLTLMR
                     RVGLRIFPMNLEQDGLDLDNLENKLKNGEQIRLIYTIPDFQNPSGSLMALAKRERLID
                     LAIRFNVSIVEDAPYRDLRYEGEALPSLFELAPDTTIQLSSYSKLISPGLRVGYAVLP
                     QNLTKTVAHYAEDTYINPSYLNHAVVYRFLQNGYMETHLRFLKTLYQKRLKCMLTTMR
                     QHMPAGNAWTEPQGGFFVGLWLASHNGPALLEKAEEQGLRLTDGRGFFVNAGENFIRL
                     PFCALTEEEIEQGIITLARLMHS"
     gene            complement(6633..7067)
                     /gene="mscL"
                     /locus_tag="PLL88_06060"
     CDS             complement(6633..7067)
                     /gene="mscL"
                     /locus_tag="PLL88_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOJ01167.1"
                     /translation="MIREFKEFIQRGNVIDLAVGVIMGGTFGKIISSLVNDIIMPPIG
                     LLFGKIDFSNLFVTLHGGSYKTLQEARNAGAVTINYGVFLNQVVEFLIVAVSLFIVIR
                     QLNRLSKAKPAPAEPTTKPCPYCFTEIPIQATRCPHCTSELP"
     gene            7534..9585
                     /locus_tag="PLL88_06065"
     CDS             7534..9585
                     /locus_tag="PLL88_06065"
                     /inference="COORDINATES: protein motif:HMM:NF011305.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HOJ01168.1"
                     /translation="MNEIIYTLARFAVNLYPYLHALALAVGSVFAISGFTDIIFDIYF
                     IFRSIRRFFLSRNWSKLTLERLEAREQQKIAIIVACWHEEAVIAKTLRNACESIHYRN
                     YDIFVGTYPNDPETQWEVDKVSREYPQVHKVVTADDGPTNKAANLNQVFEAIKDYERK
                     TGLQYEIIIMHDSEDVIHPYELLVDNYLIPRMDIVQVPVFPMALPLRHATHWTYADEF
                     AENHTKLLLVRELTGGFVPSAGVGTAFTKRAFELFALENEKKIFTPGSLTEDYELGLR
                     MNLRGYKAAFVLVHLPPEDANKRAQRRTTDWVATRAMFPLDFKRAVRQKTRWNIGIVL
                     QSWQKIGWKGNVKVRWNLFQDRKALFTTPANFLGYFVFLYFILYQILKTYFAQYMPPL
                     IVKGTLLWYLTLIATFFMIWRSLNRAYAVNKIYGLWPAITSIPRSIWGNVINFFAIMR
                     AVRIYFTTRLKKQRMQWDKTEHEFPSEVIEEERASVQGFSTLMEKQHQIVAVNEETVI
                     NEFVKAMQNGTVKEKVAALQKVDRSTGSILFPYMVKYADDPAWQVRGEFCRTCSFLRY
                     AQAVPYLRKLAFDPDWTVRSNAVRALGKLGELGERELLNILKGKDSYASEAAQVILEH
                     QEFFKQNVQRLFSEDREEIKRGLSFLEDLNCHGESKLAGQLLNLYLEGNEKEIRLFLG
                     S"
     gene            complement(9593..12100)
                     /locus_tag="PLL88_06070"
     CDS             complement(9593..12100)
                     /locus_tag="PLL88_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ01169.1"
                     /translation="MQKNVWLNEMDNPIIKIFDKLELLVVILDRNQQIIYANPHFLNL
                     TNSSPESILGKNWFNAFVHKKASETREVLSKITCEEPPVQYLDELQAKSGGFLSIRWN
                     VSGIESDDKSSHSTLCIGEDITQHMNNEASLKRQLSELAILHGITLSATTSQSMDELV
                     SGATSLLTSIFSLNNYGIYLADPTNDNLIMHDSYQGIPDKKQRDRIIPSTEGILGYVF
                     HSGEPYYSKDVLKDDTYFNFDPRTRSELAVPIKLHHQTIGVINAESEKLDAFSDEDKR
                     LLTTFSETLSTAIEKIRLFESTQQQAKEISLLYETAVATSSVLDSEKLFSTIYEKVKK
                     ILPLDSFVIVKNNKPNNEYIVVSAIENDEKLGEWEGIPFPIEPKSPFSWVILERSPLL
                     IQDLQSETTYCPPFHGKKPCRSWLGVPLVVHEKTIGAMAVQSYDPKKFSNNDLHLLES
                     LGSQIAITMENTRLLEQTQKQLERLSALHDIDLVINSSLDLRVTLNILLDQVVEKLNI
                     DAAAVLLLNQQTQLLNFAAGRGFRTRTIESYRLRLGEGISGQTTMERHLVQALDLFET
                     DEGMAYLPLLQEEGFLSFYSVPLIAKGQVKGVLDIFNRKLITPDQDWIYFLETLAGQA
                     AIAIDNTTLLENLQRSNIELSLAYDTTLEGWTRALDLRDQETEGHTQRVVEMTIRLAQ
                     LLGVPDEDLVHIRRGALLHDIGKMGIPDSILLKPGPLTEEEWVIMRKHPVFAYNLLYP
                     IAHLRQALDIPYCHHEWWNGSGYPRHLKGEEIPLSARIFSVVDVWDALTSDRPYRKAW
                     NAKKTLDYIEAQKNIQFDPQIVDLFQKLIKNELFKKL"
     gene            12328..13809
                     /locus_tag="PLL88_06075"
     CDS             12328..13809
                     /locus_tag="PLL88_06075"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOJ01170.1"
                     /translation="MVEGIKQSILLLGDQADFSEQIFTIVRDNAWCGLNLIRIEKRID
                     FGRILKNNNICLILFCVQDALYFQEALHEVQKYGYAGPLIPITRINNAEITSLAREFG
                     YQEPIPAETVNDCVLLQAIFYSIEKKRIENEINVRDAILRSVNFAAEKFLNNPDWKTY
                     IQDVLQKIGEASRVDRVYLFENVYSEEKEISHVRLIERWIAAGISAEFIPRPGEERTI
                     EEVGIASLFEEIRRTRYVKTHVRELPKKYQPLMQMKNIKSVLLVSIYTNGDWWGFIGF
                     DQCRYEREWKTLEIQELRTAGSLISAAISRQKADARMKYLATHDYLTDLPNRLLFEDH
                     FRGAMARSQRSKKWTGLYVIDLDHFKKVNDTFGHPFGDKVLIEVGKRLQKVTRASDTV
                     ARIGGDEFVILGEDLSSSNDVNLVGKKILSAFSEPIVYDHNTVQITPSIGVSIYPVDS
                     GEMEELMHFADVSLYRAKEQGNTFNVYQDGTGKQLWLDNLQKY"
     gene            14146..14349
                     /locus_tag="PLL88_06080"
     CDS             14146..14349
                     /locus_tag="PLL88_06080"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HOJ01171.1"
                     /translation="MDCNKEKNQKRCNCSFESCSRKGICCDCIRYHLQMRELPACCFP
                     DVVEGTYDRSFERFAQLVTSGEV"
     gene            14389..15660
                     /locus_tag="PLL88_06085"
     CDS             14389..15660
                     /locus_tag="PLL88_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01172.1"
                     /translation="MLFKKLSRQQIITLLILLVLSVAYYCVFIPVNSSSSEGGSAFSI
                     FSGDEYITYPYVEKMLRGDSDIHQLWGNLIIYGDYHYGYPFYFLSMLVLLPRRLVLGD
                     AFFAARTTNIFLLRQLVNVLPMILAAGVLVYYKTRFRNTVQSTLLFLLFLMVPSVVRS
                     NIWWWHPDSITFLFIALTFLFLQKDDYRLGRNFILAAVTCGLAAGTKLLGFFFFLTIP
                     VYLVITHSKRGAYWTRIIKKGLLFVFVMTVVIVLANPFLWYEGPRNEMIAIQQFKQTE
                     LEEGYTHGDPSSYQKGPSFWQWTIENWFGAVLFIVFLVFSTVWGVFQKKDFNENIYLI
                     TWSFPFTLYVLFFVAPKPDHYIFPALMPLFLAALDIPVLVREKWKNLPLSTKLLALLA
                     LVGILVILVQQIRFSIVKDLLVYQEYLIENM"
     gene            15763..16779
                     /locus_tag="PLL88_06090"
     CDS             15763..16779
                     /locus_tag="PLL88_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ01173.1"
                     /translation="MRVIIQIPCFNEEDCLLTTLHDLPRKLNGVDEVFWLVINDGSKD
                     RTVEVAIQNGADCVVSHTRNLGLSRAFQTGLEACLWLGADIIVNTDADNQYNAEDIQK
                     LIDPILQGKADYVVGARPIKEIEHFSPIKKFLQKAGSKVVSRLSQIDIEDTTSGFRAM
                     NRRTALKLNVFNEYTYTLETLIQSGQMKIATVFIPIRVNAKTRPSRLISSTMNYLRRS
                     AIIIFRSFLTYKPLKIFSTIGSIAILLAFVIMVRYLHYYFKGEGTGHIQSLLLAIILF
                     IFGTSAWMFGMQADLISVNRRLIEDVSQRVKRLESKILNESAIDLPDLTDARWDFLKK
                     KNKT"
     gene            17037..>19168
                     /locus_tag="PLL88_06095"
     CDS             17037..>19168
                     /locus_tag="PLL88_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01174.1"
                     /translation="MLLFSIILLFFLLIYFIFYYEPTPFFTTHTLVVSNISENSDDWE
                     PVEITSLSYLDGVPVATDDLSFDGEFWIADGNILLKPSSSILLKKTLIGALKLSFRSD
                     DQQGSVLVIWDGQEKTLDLGEQDDSQVLVSLSPHSWGSPNTLWLGLAVLSVISDFFVI
                     FAVVIIIALLFLTNYLDQSDTFQEKAFPFRLDWVDLLIAVALLGISIVLSMNLFDGHF
                     GEYTELSGDAGNYASFAAAKTYPTLFQNDPLLGNADNYDLYSTYHISLTQILFPVVGN
                     FGTAFMILQMPLTFMQLFGFYLLGKQMYKSRIFGLLLSALTFIFIQMNLSEFWGYVTS
                     PIPRFSFQAVLPFLLFFVLRMGGNVKRWPLLLAITGATIYVHSVSAPVWSVAIILCLW
                     FMAPRSVPFSLKCKYMLIALLVFLFVLAPFILKYFQKTAFGEASAYSIEEVYSIIDYR
                     LAQGMVDFQTAIGDFFAVLTGNGLRVFFFAMSLLSLAWMNFMARDPDEKKKVIAVTMW
                     LAGVLSVSVLFPMVDFAIAEVLDRNPLEIQFLRAMRNFFPLFYILFLWPFASMYNTVK
                     SKDPASTLKKVSCVLLCIGFLALWCNDVQFLATPLISRTLGCWNDGKVVCPENEELAV
                     RSEFFRLIKEETPEGASILSDDLAIRYYSLRPLAFAKKDGASFSFSNHNALLAWYGYS
                     RAYDELWKYRDDPGAYVRAYKDFAQMVKA"
BASE COUNT         4773 a         4207 c         4715 g         5473 t
ORIGIN      
        1 cagttttctg gcaggcatcc gtttagatgc agcccgacca ctctggggaa ggtgaattcc
       61 atcggatacc ccagcatggc agccagcgcc tcgatgcctc ccacgcccca gccgaccacg
      121 cccagcccgt tgaccatagg ggtgtgcgaa tctgttccca acacgcaatc cgggataatg
      181 gtcagattcc cttcgctctg tctgatggat gccacttcag ccagatactc caggttgatc
      241 tggtgaatga taccgttccc cggtgggatg atatggagat tctgaaaagc ttgttcgcac
      301 cagcgcagaa attcgtaccg ttcgcgattg cgtcgatatt ccagctcttc gttcaattcc
      361 tgtgctcgtt cgcagccggt atggtcaacc tgcaaggaat ggtcaaccac caggtcagta
      421 cggatcactg gattgatgga gcgggggtcg atatttcgtt ttgcagccgc tgcccgcaat
      481 gctgccaggt cattcagcac tggtatgccc gtgaaatcct gcaggatgat gcggttgggc
      541 aataaaagca ccgtatcctg gtcggtgtgc tcgctttgta aaaatgtcag aatatgctgt
      601 cgggtgaaac ccttttttcc gcaatttcgc agcatgcctt ccagaaggat gcgcatgcag
      661 tagggtagtt tttcgatggt gccgaatccc tgctcctgga gtttgggcag gctatagaac
      721 tgaaatgttt gctggcgcac actgatggta tcgatgacac tgttgaattc attttccata
      781 ttgggatctt ctctctatgc catatttttc attacgagcg cagaaaattg cgaggtgcta
      841 acaagggtgc tgccgggcaa ctgcgtatgc agatcaaagg tgacatggcc tgcctggatg
      901 gttttctcaa taccatcacg aagcaacgca gccacctcgt gccatccaag aaaatcgaac
      961 agcatcgctg ctgaaaggat cagcgaactg ggattggctt tgtccattcc ggcaatatct
     1021 tcggctgacc cgtggtttgc ttcgaacagg ccccaaccca attcggaatt gatattggct
     1081 cccggagaaa ctccgattcc tccgaccaat gcagcaaaag cgtctgaaat gtagtcgccg
     1141 tttagattgg tggttaccac cacatcaaaa tcagcagggc tcacaatggc tttcgcaaag
     1201 gcattgtccg caatcaggtc atccacaaag atcatcccgc gccgtaatgc ttcagctttt
     1261 tcactatccg ctttcccttt tcctttttta tcaaaagtcc tcttccaggc gcgcttcgag
     1321 taacagacat cggaaaattc agattcggct atctcatatg cccaattgcg gaatgcccct
     1381 tcggtgtatt tcaaaatatt tcctttgtgg acgatgctga tcttatgcct tttgtttttg
     1441 atggcatact gaatggcagc gcgcatgatg cgctgcgagg cttgctttga aatcggcttg
     1501 agatcgatgc ccacttcctg tgcataacgc aatttggcga aatcattcgg aaatctcgtt
     1561 tttaataagt caaggattat cgaattctcc tcagtttcag ccggaaattc gatgcccgcg
     1621 tacaaatctt cggtgttctc gcgaaagacg atcacattca cgttttccgg atgagatagt
     1681 ggagaaggca agcccgggaa ccacctcacc ggtctgacgc aactgtagag gtccagttct
     1741 ttgcgcagcg tcacgttcaa cgaggtatat cctcctccca cgggcgtcgt taatggacct
     1801 ttgatgccta ccttgtgttt ttttaactgt tctatgcttt caggtgggag atatgtgccg
     1861 ctctctcgga acgctttttc tccggcggct atttctagcc agtcaacagc acgtgtgcct
     1921 gaataggctg attcgatagc gcgttccagc acgcgccggg ttgcattcca gatatcgctg
     1981 ccgatgccat ctccgatgat gtaggggatc gtgatgtgct ctgctttgtc ttgtgccatg
     2041 ctgtaccttt ctagaatcct gtgggtaatt ttaacattcg aaaaatgctt gtaaaaaatt
     2101 gcaaggatag gtacaatata ccatattatt tgggacactg tcctataaaa cggcacaaag
     2161 aggaaagatg actaccgaaa aagttcaaat tttgcaacga gtgattgata tattggattg
     2221 tttcacgtta gagagacctt ctttgggggt gcgggaagtt gcccgtatgg tcaacctttc
     2281 gagcagcacc actggcagat tgatggttgc tatgaaagat ctgcgtattc tcaatcagga
     2341 tcccgtcaac aaaacctatt ctatcggttc gcgggtgctg acctgggcag gcatttacac
     2401 cgcaacttct gatctgcgcg cggtcgctct gccggtgatg gtcaatctgt tggaacgcac
     2461 tcgcgagacg atcagtttgt atgtgttgga agggaaggaa cgggtgtgca tcgagcggct
     2521 ggaaagccct gaaccggtgc gtattacagc ccgtgtaggg aggcgcatcc cgctctatgc
     2581 cgggtcagcc ggaaaatgca tgctggcttt catgccggag gaaaaatgtg aggagatcct
     2641 caatcaaatc gtgttgacgc ccctcacgca ggctactatt cgcgataaag atgctttgcg
     2701 cagagatctg gcgaatatac gggaagaggg ttgtgcggtt agctatgggg aatggattct
     2761 ggaagcgtct ggagtggctg cgccgatttt caacgaattc ggcacgctgg ttgccgcact
     2821 taccatttct ggtcccgggc agcgctttgt ggaggagaag atacaagatt ataaagagct
     2881 tgtgaaagaa aaagcacggg agatctctgc caaattgggt tacatgccac gctaaaggag
     2941 tgatgggaga attgatgcga tggagcttgc aaagaaatta gaagggaaga tcagggaggt
     3001 gtatgaacag gtacgttata tgctggcggt ctctggtgaa accattatca gtcaggtctc
     3061 gctccagcag tttttcagcg gcaatcgtgg cgttgatcta ttaatgagcg atgtctcatt
     3121 cgtagacccg tttgatggtt tgcattaccg gggaattccg atcgatcagg tgttgaatga
     3181 gttgccaaaa cctgatggca acgcattccc ctacgccggc ggattatacg cttttctctt
     3241 gaccggaagt ttcccaacgc ttgccgaagc tttagaagtt gaggacgtgt ggagagcaag
     3301 agcatccatt ccagcctatg ttgtagaaat tttaaaagcc atgccctcag attcgcaccc
     3361 catgaccatg ttttcacaag ccattctcgc tctgcaaggg caatcgaaat ttgcaaagac
     3421 ctgttctgat ggattgcgca gggaggatca ttggaaggtc atgctggaag atagtctgga
     3481 cctgacagcc aagacgcctg ctctggcagc agccatttat aacatcaagt atggaaacgg
     3541 ctctcccaga gagatcgacc ctgccctgga ttggagcgct aattttgcgc acttgatcta
     3601 taaggaatgg gatgtgcaat atcaggatct gtgccggctt ttcttttgtt tgcacgctga
     3661 ccagggggtg ggtaacgttt cagcgcatgc ggcttgcctg gttaattcag ccctttcgga
     3721 tgtttattat tcctgttcag ccgccatgaa cggtctggct ggtcctttgc atgggaaggc
     3781 gaaccagggt tgcctggaat ggttgctgaa gatcagggag aagttcaata acgctcttcc
     3841 gacaatggat gagttggaaa gttttgtctg ggataccttg caggagggga aagtgattcc
     3901 gggatatggg catgccattc tgcgttgcac cgatccgcgt ttcactgccc agtatgagtt
     3961 cggcaagcaa tatctgcctg atgatgacct ctttaaattg gttgatatgt tataccaggt
     4021 tgttccggcg gctcttaaaa aacagggaaa agccaagggg atctggccca acgtggatgc
     4081 cattagcggg atcctgcagt accattgcgg cttaaaacag tttgattttt acactgtcct
     4141 ttttggagtg ggtcgcatgc tgggaatcac tgccaacctg gtttggaatc gggctttgat
     4201 gctaccgctt gaacgcccgc aatcgatcac tctcgaaatg gtgaagaatc gcctgcatag
     4261 aaatctggat tttcctgcca tgaaccatga ttttcgttat taaatcgtag aaaataaaga
     4321 atatcaggtt ccctctctct gggtataatc acgcatgcgg gtgaacatgc aggtacagtg
     4381 cgagcaatgt ggtagaaaag aagattaccg ccctgccgag tggcgttgtg cgtgtggtgg
     4441 ggcgtggatt ctgcgcgcat cccaaccgct tgtgccagaa ttgattgcaa aagacaccaa
     4501 ctcactctgg cgctacagcg ctttctttcc acctgaaatt cagaaaatgc gaaacacact
     4561 cgcagcgggg ttcaccccct tgctgccatg caggttgcag gatagagatg ttctgctaaa
     4621 aatggactat ctcaacccta ccgcttcgtt caaggatcgt ggtgtggaat tgatgatgaa
     4681 tgccatggcg gcagcgggta tagagcgcgt ggtcgaagat tcgtcgggca acgcaggggc
     4741 ttcagtggcg gcttattcgg cttgcctcgg aatgaaagct tccatttttg ctcccgctct
     4801 tgcttcaccc atgaagctgg ctcaaatttc tctgtacgga gctgaggtgc gcctcatccc
     4861 cggcgcacgc cagaatgcca gccatgccgc cctggaagag ttggaaaagg gagccatcta
     4921 cgcatcgcac gcttacaacc ctgtgtatct gttggggcag cagacggcag cctgggaggt
     4981 gtgggagcag agtggctacc atgctcctga ttgggtggcg gtgccggtgg gtcagggtgg
     5041 aaatctgctt gggtactatg aaggctttcg agcgctgctt gttgcaggct tgatcgaaaa
     5101 aataccaggc atcattgcgg tacagcctga acggttggct ccgttatgtg acgcatatca
     5161 tgagggatgg gaggaatggc atactgttcc cccgccgcag gatggaagcg ttgcagaagg
     5221 tttggcgatt acccaaccgg tgcggtggaa acagatcatc gctgcgatca aggagagcca
     5281 tggcttttgt gtgaaagtag ccgaagagga tattttgccc gctcagcgca ggctggcggg
     5341 aaaaggcttc tatgttgaac caagcagtgc ggtgattgtt gcagcgttga atgaaatcta
     5401 tgagaatgtc gaaaggaaac aaacgatcgt tctttcattg acgggcagtg ggttgaagtc
     5461 tggtccgcta acctcgcagt gaaggagatt gaagaagcat atccatgaac ccaatctttt
     5521 ttacccgtgg agttccccct cgcgaaagta tcccaagcag tgtactggct gagtgtgcca
     5581 aaaccgccat cgctgaagaa ggagcggata ttctccagta ttcctcttca gcaggatacc
     5641 cgcctttgcg agaggtgatc gcaagggcaa aaggtataag catggagcgc gtgattctcg
     5701 gacagggttc cttgcagatt ttggatatcc tgctgcattg tttgctgtac cccggtgatg
     5761 ctgttgcggt ggaacagccc acctatgacc gtgtgctcac tttgatgcgg cgtgttggtt
     5821 tgcgcatctt tcccatgaat cttgaacagg atgggttgga cctggataac ctcgaaaaca
     5881 aattgaaaaa tggagaacag atcagactga tctataccat tcccgatttt caaaatccca
     5941 gtggatcgct tatggctttg gcaaaacgcg agcggttgat tgatctggcg attcgtttta
     6001 atgtttccat tgtggaagat gctccctatc gtgatctgcg ctatgagggg gaagcgcttc
     6061 catcgttgtt tgaacttgcc cccgatacca ccattcagct ttcgtcttac agtaaattga
     6121 tcagcccggg tttgcgggtc ggctacgcgg ttctgccgca aaacttgacg aaaacagtgg
     6181 cgcattatgc cgaagatacc tatatcaatc caagttatct aaaccatgcc gttgtttatc
     6241 gtttcctgca aaatggctat atggaaacac atctgcgatt cttgaaaacc ctgtaccaga
     6301 agcgcttgaa gtgcatgtta accacaatgc ggcaacatat gcccgcagga aacgcctgga
     6361 cggaacctca aggcggattt ttcgttggtt tgtggctggc gtcgcataat ggaccggcat
     6421 tattggaaaa agcggaagaa caagggctgc gtctgacgga tggtcgtggt ttctttgtca
     6481 acgctggaga gaactttatc cgcctcccgt tctgcgcact gactgaagaa gagattgaac
     6541 agggaatcat cacattagcc cggctgatgc attcttaacc gtcttcagca tgccttttcc
     6601 ttactattaa ttaatttacc cacagtcaga tgttacggaa gttccgaggt gcagtggggg
     6661 cagcgagttg cctgaatggg tatttctgtg aaacagtacg ggcatggttt ggtggttggt
     6721 tccgcagggg caggttttgc ttttgacaac cggtttagct ggcgaatcac aataaataag
     6781 gaaacagcga cgatgagaaa ttcgacgacc tgatttagaa aaaccccata attgatggta
     6841 actgcgccgg cgttccgggc ttcttgaagg gttttatagg atccaccatg cagggtcacg
     6901 aatagattgc taaagtctat tttcccgaac agtaatccaa tgggtggcat gatgatgtcg
     6961 ttgaccagcg aactgatgat cttgccgaac gttcccccca tgatcacgcc cactgccagg
     7021 tcaatcacat ttcctcgttg aatgaactct ttgaattctc tgatcatttt tgttctcctt
     7081 atgattactt cttcattgta tacgtatcga tacccagatg atatcatctt gaacgatatg
     7141 agggacaaaa tacgtatctt cctgttcatc cagggtggat tcccggcaaa ttgtgccatg
     7201 attgattgcg ctttttctcc atcgtcaccc tccaaaatga tcgaaagatc gcaaatgttt
     7261 catctgaagg tacgatgggg aaaattccaa tagccgcatc gttgggtcga tgcctgttca
     7321 aaacttcttt tgctttggaa caggttgata atgaaaccag aaataaaagt agaatatata
     7381 ttaacgaagt tctgataatc cacatgtcca tcttcggtat aattgtgcgg ttgttcccaa
     7441 ttcaggggag aagcaaaaat tttattcatt ctttgttgtt ttttgagctt ctataaaaag
     7501 ttgtgaaatt aaacatatta tccatgagat gaaatgaacg aaataattta taccttagcc
     7561 cgttttgctg tgaatttata cccatacttg cacgctttag cgcttgcggt tgggtctgtg
     7621 tttgccatta gtgggtttac cgatattatt tttgacattt actttatttt tcgctccatc
     7681 cgtcgttttt tcttatctcg caactggtcc aaactaacgc tggaacggct ggaagcccga
     7741 gagcagcaaa agatcgctat tattgtggca tgctggcatg aagaggcggt gattgcaaaa
     7801 acgttgagaa atgcctgcga atcaatccat taccgcaact atgatatctt cgttggcacc
     7861 tatccgaacg atccggaaac acaatgggaa gttgataagg tcagtcgcga atatccgcag
     7921 gtgcataagg tcgtgaccgc agatgatgga ccaaccaaca aggccgcaaa tctaaaccag
     7981 gtgttcgagg ctattaagga ttacgagcgg aagactggct tacagtatga gatcatcatc
     8041 atgcatgact ctgaagatgt gatccatccc tatgaactgc tcgtcgataa ttacctgatc
     8101 ccacgcatgg acattgtgca ggtcccggta ttcccgatgg ctttgccttt gcgccatgcc
     8161 acgcattgga cttatgctga tgagtttgcc gaaaatcaca ccaaattgtt gctggtgcgt
     8221 gaattgacgg gtggctttgt gccttcggcg ggtgtaggaa cagccttcac gaaacgtgct
     8281 tttgagttgt ttgcattgga aaacgaaaag aagatattca ctcccggttc attgactgaa
     8341 gattatgaac ttgggctgcg aatgaacctg cgcggttata aagcggcgtt cgtgcttgtc
     8401 catcttcccc cagaagacgc aaataagaga gcgcaaagac gaacgactga ttgggttgca
     8461 accagggcga tgtttcctct ggactttaaa agagccgtga ggcaaaaaac acgctggaac
     8521 attggcatag tgctgcaaag ctggcaaaaa atcggatgga agggaaacgt gaaagtgcgt
     8581 tggaatcttt ttcaagatcg taaagcgctg ttcaccaccc ccgcaaactt cctgggatac
     8641 ttcgtttttc tctacttcat cctttatcag attcttaaaa cgtactttgc gcaatatatg
     8701 cctcctttga tcgtgaaggg cacgctctta tggtatttaa cccttattgc aacctttttt
     8761 atgatctggc gatccctcaa ccgtgcctat gcggtgaaca agatctacgg attatggcca
     8821 gcgatcactt ccattccccg ttctatttgg ggcaacgtta tcaacttttt tgcgatcatg
     8881 cgggctgtgc gcatctattt caccacacgt ttgaagaaac agcgcatgca gtgggataaa
     8941 acggaacatg aattcccatc ggaagtcatt gaggaagaac gcgcgtcagt gcaaggcttt
     9001 tccacgttaa tggagaaaca gcatcagatt gttgctgtca atgaagaaac tgtgatcaat
     9061 gaattcgtga aggctatgca aaatggcacg gtgaaagaaa aggtggcagc acttcaaaaa
     9121 gtagatcgtt ccacaggatc cattctgttt ccctatatgg tgaaatatgc cgatgatccg
     9181 gcgtggcagg ttcggggaga attctgccgc acatgcagtt tcctgcgcta tgctcaggcg
     9241 gttccctatc ttcgtaaatt agcgttcgac cctgattgga ccgtgcgtag caatgcggtt
     9301 cgggctctgg gaaaacttgg tgaattgggc gagcgcgaat tgttgaatat tctcaaggga
     9361 aaggacagtt acgccagtga agccgctcag gttatcctgg aacatcagga attctttaag
     9421 caaaatgtac aacgattgtt ctcggaagat cgagaagaga tcaaacgcgg gctgtcattt
     9481 ctggaagatc tgaactgtca tggcgagtcg aaactggctg ggcagctatt aaatctctac
     9541 ctcgagggca acgaaaaaga gatccggctt ttcctgggta gttgaacagt ttttataatt
     9601 ttttaaacag ctcgttcttg attaatttct gaaacagatc gacgatctgg gggtcaaact
     9661 ggatgttttt ttgcgcctca atgtaatcca gggtcttttt ggcgttccac gcttttcggt
     9721 aaggtcgatc cgaggttagt gcgtcccata cgtccaccac tgagaagatc ctggctgaaa
     9781 gcggtatttc ttcccctttc aaatgtcttg ggtaaccgct tccattccac cattcatggt
     9841 ggcaataagg aatgtcaagc gcctgacgca gatgcgcgat aggatagagc agattgtatg
     9901 caaaaaccgg atgcttgcgc atgattaccc attcttcttc tgtgagcggt ccaggtttca
     9961 gtaatatgga gtctggtatg cccattttcc cgatatcatg cagcagcgca cctcgacgga
    10021 tgtgtaccag gtcctcatct ggaactccga gcagttgtgc caagcgtatg gtcatttcga
    10081 ccacacgttg cgtatgcccc tcggtttcct ggtcgcgcag atccagtgcg cgcgtccagc
    10141 cctccagggt tgtgtcgtat gccagagaca gttcgatgtt ggaacgttgt aagttctcaa
    10201 gcaaggtggt gttgtcaatg gcaatagcgg cttgccccgc cagcgtttcc agaaaataga
    10261 tccaatcttg atcgggggtg atcaattttc tgttgaaaat atccaacacg cctttaacct
    10321 gccctttggc aatgaggggc acgctataga aactgaggaa cccctcttct tgcagcaaag
    10381 gaaggtaagc catcccctca tcggtttcaa agagatctag tgcctgaacg agatgccttt
    10441 ccatggtggt ctgtccagaa attccctctc ccagtcgcaa tcggtaagac tcgatggttc
    10501 tcgttctgaa tcctctgccg gcagcaaagt tcaacaattg cgtttgctga tttaacaata
    10561 gaaccgccgc tgcgtcgatg ttcaattttt caaccacctg atcgagcagg atgtttaatg
    10621 tgacgcgaag gtcaaggctt gaattgatca ccaggtcgat atcgtgcagg gctgatagtc
    10681 gttccaattg cttctgtgtt tgctccaaaa ggcgggtgtt ctccatggtt atcgctatct
    10741 gggatcccaa cgattcaaga agatgaagat cgttattgct gaattttttc gggtcgtagg
    10801 attgcacggc cattgcacca atggtctttt catgcaccac caggggaacc cccaaccagg
    10861 aacggcatgg cttttttcca tgaaagggtg ggcaataggt agtttcgctt tggaggtcct
    10921 ggatcaatag ggggctccgt tccaatatca cccagctgaa ggggctcttc ggttcgatcg
    10981 gaaatggaat gccttcccat tcacccaact tttcatcgtt ttcgattgct gaaaccacaa
    11041 tgtactcgtt gtttggtttg ttatttttta ctatgacaaa ggaatcgagg ggtaatattt
    11101 tctttacctt ctcgtagatg gtgctgaaca gcttttctga atccaaaacg cttgatgttg
    11161 ccacggcagt ttcatacagc agactgattt cttttgcttg ttgttgggtg gattcgaaga
    11221 gtctgatctt ttcgatggca gtggagaggg tttccgaaaa agtggtgagc aatcgtttgt
    11281 cttcatcaga aaatgcgtcc aatttttcgc tttcggcgtt gatcactcca atggtttgat
    11341 ggtgaagttt tatcggaaca gccagttccg atcttgttct ggggtcaaaa ttgaaatacg
    11401 tatcgtcttt cagaacatct ttgctgtaat acggctcacc gctgtggaat acgtatccca
    11461 gaatcccttc tgttgaaggg ataatgcgat ccctttgttt cttgtcagga ataccttgat
    11521 aagaatcgtg cataatgagg ttatcatttg tcgggtcagc cagatagatg ccatagttgt
    11581 ttagtgaaaa gatgctggtc aacaagctgg ttgcaccgga gacgagctca tccatcgatt
    11641 gagaggttgt ggctgacaaa gtaatgccgt gcaatattgc caattcagat aattgccgct
    11701 tcagagatgc ttcattgttc atgtgttgtg taatatcttc tccaatgcaa agtgtgctgt
    11761 gggaggactt gtcatcgctt tcaatcccag aaacgttcca tcgaatcgag aggaagcccc
    11821 cactttttgc ttgaagctca tcgagatact gaaccggggg ctcttcacag gttatcttcg
    11881 aaagaacctc acgggtctct gatgcttttt tatggacgaa agcgttgaac caattctttc
    11941 ccaagattga ttcaggggaa gaattggtca ggttgagaaa atgtgggttg gcatagatga
    12001 tttgctgatt tctatccagg atgaccacca ggagttcgag cttgtcaaaa attttaatga
    12061 ttggattatc catttcattc aaccagacat ttttctgcat aaaaaaacgc ctgcgcatgc
    12121 tgctgtaaac cattgttgta gcaaagcatt gcgtagaggc gccctccacc gagtattgtt
    12181 atcattatat atgaaaaaaa ttctaatagt ttttcctttt tattttctgt ataatagcga
    12241 tgtgtatggt ccatgaatgt tcatcgaaga gaaaatcagt ggggatgaga gcatatacaa
    12301 caaatagaga taagtcgata gtgaggtatg gtggagggca taaaacagtc tattttattg
    12361 cttggtgacc aggcagattt cagcgaacaa atatttacta ttgttcgtga taatgcctgg
    12421 tgtggtctca atttgattcg aatagagaaa aggatcgatt ttggtaggat cttaaagaat
    12481 aataatattt gcctgatctt attttgtgtg caggatgctc tgtattttca agaagcattg
    12541 catgaggtcc aaaagtatgg ttatgcagga ccattgattc ctattacccg aataaacaac
    12601 gcagagatca ccagccttgc gcgcgaattt ggttatcagg agccgattcc ggcggaaacc
    12661 gtgaacgatt gtgttctttt gcaggcaatt ttttattcca tcgaaaagaa aagaattgag
    12721 aatgaaatta acgtgcgcga cgcgattctg cgctcggtca attttgcggc agagaagttt
    12781 ctcaataatc cggattggaa aacgtacatc caggacgttc tccaaaagat tggtgaagcc
    12841 agccgggtgg atcgcgtata tctttttgag aacgtgtatt cagaagaaaa agaaatctcc
    12901 cacgtgcgtt tgatcgagcg ctggatcgct gcggggattt ctgccgaatt tattccaaga
    12961 cctggagaag agcggacgat cgaagaagta ggaattgctt cactatttga ggagatacga
    13021 cgaacgcgct atgtgaaaac ccatgtccgc gaactgccta agaaatatca accgttgatg
    13081 caaatgaaaa acattaaatc tgttctcctg gtctctattt atacgaacgg agattggtgg
    13141 ggttttatcg gatttgatca atgcaggtat gagcgcgaat ggaaaacgct tgagattcaa
    13201 gaactgagaa ccgccggaag tctgatttca gccgccattt cacgccaaaa agcggatgcg
    13261 cggatgaaat atctggcaac ccatgattat ctgaccgatc tgcccaacag actgctgttc
    13321 gaagatcatt tccgcggagc aatggcacgc tcacagcgtt cgaaaaaatg gaccggacta
    13381 tacgtcatcg acctggatca ttttaaaaag gtgaatgaca cgtttgggca tccgttcggt
    13441 gataaagtgc tgatcgaggt gggaaagcgc ttgcaaaagg ttacccgtgc cagtgatacg
    13501 gttgcccgaa ttggagggga tgagtttgtt attctgggcg aagacctttc atcgagcaac
    13561 gacgtgaatc ttgttggaaa gaagatcctg tctgcattca gtgagccaat tgtatacgat
    13621 cataatacgg ttcagattac tccaagcatc ggtgtgagca tctaccccgt tgatagcggt
    13681 gaaatggaag aactgatgca tttcgctgat gttagcctgt acagagcaaa agaacagggt
    13741 aatacgttta atgtttatca ggatggtacc ggaaagcaat tatggctcga caatctacaa
    13801 aagtattgat cggcatgacc ggcacgatcc tttttttata aaaaatcgct gcaaaaacgg
    13861 atctgattgc tgttatttta agtgactggt tggagaaaac agacagtgct ccgatggtcc
    13921 accttttgtt catcctgaac aaagggagag actttatacg aagttgaaaa gtcgctattg
    13981 aagatcatag ctggcaggat tttcctctgg ttgcaaaaag aaagtcgcag ggagcgatcg
    14041 aggtatattc tttgaaagaa tgcggaacat ccaatacaat gaacgggtcg tttacaatag
    14101 aagatccttt cttgatgaga taaaccggat cttccaggag atgccatgga ttgcaataaa
    14161 gaaaaaaacc aaaaacggtg caattgcagc ttcgaatctt gttcccgaaa aggcatttgt
    14221 tgtgattgca tccgttacca tctacaaatg cgcgaactgc ctgcctgctg tttcccggat
    14281 gtcgttgagg gcacctatga tcgttccttc gaacggtttg cccagctcgt cacgtcgggg
    14341 gaagtataat catgaaatgg tggtaacagt ctggaaagag gaattcattt gttattcaaa
    14401 aaactttcac gacaacagat tatcacactg ttgattttgc tggtgctttc tgtagcgtac
    14461 tattgcgttt tcattccggt caattcctct agtagcgaag gagggagcgc tttttcgatc
    14521 ttttcgggtg atgaatacat tacgtaccct tatgttgaaa aaatgttaag aggcgatagt
    14581 gatattcacc agctctgggg aaatttgatc atctatggcg attatcatta tgggtacccg
    14641 ttttacttcc tttctatgct ggtgctgtta ccgcgtcgtc ttgtccttgg ggatgctttc
    14701 tttgcagcca gaacgaccaa tattttcctt ctgcggcagt tggtgaatgt actgccgatg
    14761 atcttggctg ccggcgtcct ggtctattac aaaacgcgtt tccgcaatac tgttcaatct
    14821 accctgcttt ttcttctttt tcttatggtg ccaagcgtgg tgcgcagtaa catctggtgg
    14881 tggcatcctg attcgatcac atttctattc atcgcattga ctttcctttt tctgcagaag
    14941 gatgattatc gactgggcag aaactttatt ctggcagcgg tcacttgcgg tttagccgcg
    15001 ggaacaaaat tgctcggatt tttctttttt ctcactatcc cggtttattt ggtgatcacc
    15061 cattccaagc gtggagccta ttggaccagg attataaaaa aaggactgct ctttgttttc
    15121 gtgatgactg tcgttatcgt cctggcgaat ccgtttctgt ggtatgaagg tccccgaaac
    15181 gagatgatcg cgattcaaca gttcaaacaa actgagctgg aagaagggta tacccacgga
    15241 gacccctctt cctaccagaa gggaccttct ttctggcagt ggaccataga aaattggttt
    15301 ggagcggtgc tcttcatcgt ttttctggtt ttttcaaccg tatggggcgt ttttcaaaag
    15361 aaagatttca atgagaatat ctatttgatc acctggtctt tcccgtttac gttgtacgtg
    15421 ctcttctttg tggcacccaa accggatcac tacatttttc cagcgttgat gcctctgttt
    15481 cttgccgccc ttgacattcc cgttttggtc agagaaaaat ggaagaacct gccgctatcc
    15541 acgaaactct tggcgctcct ggctcttgta ggcattcttg ttattctggt gcaacagatc
    15601 cgcttctcga tcgtgaagga tctccttgtg tatcaagaat atttgataga aaatatgtag
    15661 cgattgcctg aatgcccaac catgtctttt tcatggatcg tggcgaacgg acaatttaat
    15721 atagtatagt aataagaatc ataaatccaa acaggaataa tcatgagggt tattattcaa
    15781 attccatgct tcaatgaaga agattgttta ttgaccacat tgcatgattt acccagaaaa
    15841 ctaaatgggg tggatgaagt gttctggctt gtcatcaatg atggaagcaa agatcgcacc
    15901 gtggaggtcg ccattcagaa tggggcagat tgtgtggtct cgcacacgcg caatctggga
    15961 ttatcacggg cttttcaaac ggggttggag gcttgcctgt ggttgggcgc tgatattatt
    16021 gtcaacacag acgctgacaa ccaatacaac gcagaagata ttcagaaact catcgatcca
    16081 attctccagg gaaaagccga ttacgtggtt ggagcgagac cgatcaagga aattgaacat
    16141 ttttctccca taaaaaagtt tcttcagaaa gccggcagta aagttgtgag ccgattaagc
    16201 cagatcgata ttgaagacac gaccagcggc ttccgtgcca tgaatcgaag aacagccctg
    16261 aagttgaacg ttttcaacga atatacctac actctcgaaa cgctcatcca atctggtcaa
    16321 atgaagatcg ccacggtctt tattccaatc agggtaaatg cgaaaactcg cccttcgcgg
    16381 ctcatatcat cgaccatgaa ttatctgaga cggtcagcga tcatcatttt tcgcagtttc
    16441 ttgacctata aaccgctcaa aatatttagt accatcggtt ctatagcaat tcttttggca
    16501 tttgttatca tggttcgcta tcttcattac tatttcaagg gggaagggac aggtcatatt
    16561 caatctcttt tattggctat catcttattt attttcggaa catctgcatg gatgtttgga
    16621 atgcaagctg atctcatatc ggtcaaccgg cgattgatcg aagatgtttc tcaaagggtg
    16681 aaaagacttg aatctaagat tctaaatgaa tccgcaatag acctgcctga cctgacagat
    16741 gcccggtggg atttcctaaa gaaaaaaaat aaaacatgac gaaaactcac aagaataaca
    16801 gggatcttcc taagaacgtt tcgtttttga tcattgctct gatcattgct ctgatcgttg
    16861 cattcttttt ctcttatagt ttatctgttc actttctgga ttggtatgca caacccgtta
    16921 ttgcaaaagt agttcttcta attgggttaa ccgcaatact tggtagcatt ttttatttta
    16981 ttcaaaataa aattctaaaa aaggttcaac ccgtttccgg aaatttatca cggtatttgt
    17041 tgttattctc aatcatcctg ctttttttcc tccttatcta tttcattttt tattatgagc
    17101 caaccccatt tttcactact cacacgctgg ttgtttccaa catcagcgag aattctgatg
    17161 actgggagcc agtggagatc acctcgctat cctacctgga tggggttcct gttgcgacag
    17221 acgatttgtc gttcgatggc gaattttgga tcgcagatgg gaacattcta ttaaaaccct
    17281 cgtcttcgat tctgcttaaa aaaacgttga ttggagcttt gaaactttct ttccgctcgg
    17341 atgatcaaca gggcagcgtg ttggtgattt gggatggtca ggagaaaacc cttgatctgg
    17401 gtgaacaaga tgactcacag gtccttgttt cgctgtcgcc gcattcatgg gggagtccaa
    17461 atacgctgtg gcttggtttg gcagtcctat cggtaatttc agattttttt gtgatttttg
    17521 cggtggtcat catcattgcg cttcttttct tgacgaatta tttagaccaa agcgatactt
    17581 tccaagaaaa ggcttttccc tttcgattgg attgggttga tcttttaatc gctgttgcct
    17641 tattagggat ctcgatcgtt ctatccatga atctttttga tgggcatttt ggagaataca
    17701 cagaactgtc cggcgatgct ggtaattatg cgtcgtttgc ggctgccaaa acatacccaa
    17761 cattgtttca gaatgaccca ttattgggca atgcagataa ttatgatctg tattccactt
    17821 accatatttc cctaacgcaa atcttgttcc ccgtggtagg gaattttggc acggcattta
    17881 tgattttaca gatgccattg accttcatgc agttgtttgg gttttatttg ttggggaagc
    17941 aaatgtataa aagccgtata ttcggtttgc tgctgtctgc gttgaccttt atttttattc
    18001 aaatgaatct tagtgaattc tggggctatg taacttcccc cattccacgt ttttcatttc
    18061 aagcagttct cccctttttg ttgtttttcg ttctaagaat gggcggcaat gtcaaacgtt
    18121 ggcctcttct cttggcgatc acaggggcga cgatctatgt tcattctgtc agcgcaccgg
    18181 tttggagcgt agcgatcatt ttatgcctgt ggttcatggc tccccgctct gtcccttttt
    18241 ctctgaaatg caaatatatg ttgatcgctt tattggtatt cctgttcgtt ttagcgccat
    18301 ttatcttgaa atatttccaa aagacggcat tcggagaagc aagcgcctat tctattgaag
    18361 aggtttactc gatcatcgat tatcgcctgg cgcaggggat ggtggacttt cagactgcta
    18421 ttggcgattt tttcgctgtt ctcaccggca atggactccg cgtattcttc tttgctatga
    18481 gtttgctttc attagcctgg atgaatttca tggcgcgcga tcctgacgag aaaaagaagg
    18541 tgattgcagt caccatgtgg ctggcaggcg ttctcagcgt cagcgtcctt tttccaatgg
    18601 tcgatttcgc gattgctgaa gttctcgatc gaaatccgtt ggaaatccaa tttttaaggg
    18661 caatgaggaa tttctttccg cttttttata ttcttttcct gtggccattt gccagcatgt
    18721 acaatactgt gaaatcgaag gatccggctt caacattaaa aaaggtcagt tgtgtgcttc
    18781 tctgcattgg ttttcttgcc ttgtggtgta acgatgtcca atttctggcg acccctttaa
    18841 tttcacgcac cctgggctgc tggaacgatg gaaaagtcgt atgccctgaa aatgaagagc
    18901 tcgccgtccg tagtgagttc ttcaggttga tcaaggaaga gacgccggag ggcgcatcga
    18961 tattgagtga tgacctggcg atccggtact actccttacg accgctggct tttgcgaaaa
    19021 aagatggggc atcattttca ttctcaaacc acaatgccct gcttgcatgg tatgggtatt
    19081 cgcgcgcata tgatgaactg tggaagtatc gcgatgaccc cggtgcttat gttcgagctt
    19141 acaaggattt tgcccaaatg gtcaaagc
//
LOCUS       DAOSDS010000039        18524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1229_length_18524_cov_4.640695, whole genome shotgun sequence.
ACCESSION   DAOSDS010000039 DAOSDS010000000
VERSION     DAOSDS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18524
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1229_length_18524_cov_4.640695"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PLL88_06100"
     CDS             <1..1288
                     /locus_tag="PLL88_06100"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOJ01175.1"
                     /translation="FVYAQDFTVKGGALGEMQSSKICRVMDLAYENGAPIIGLIDSGG
                     ARIQEGVYSLGGYGEIFRRNSQYSGIIPQISIILGPCAGGAAYSPALTDLIIMVEKQS
                     YMFLTGPNVIKTVTGEEVDFEELGGANVHLSTSGTASLTAKTESDALRLCRTILNYLP
                     ANYAENPPFVAPSDDANRVDRELNAIVPLDSSVPYSMHDVINRIVDRSSFLELQATFA
                     PNAIIGFARMDGGAVGIVAQEPSIIAGVIDINASDKICRFVQFCDCFNLPIITFVDSP
                     GFLPGVNQEHGGIIRHGAKVLFAYSTSSVPKISVVTRKAYGGAYIVMSSKYLGTDFTF
                     AWPTAEIAVMGPEGAANILYANQIKNAEDPAKERLAVTELYKEKFLNPYAAAHAGHID
                     DVIEPSQTRPIIISALKTLHQKQVQHPMRKHGNAPV"
     gene            1303..1638
                     /locus_tag="PLL88_06105"
     CDS             1303..1638
                     /locus_tag="PLL88_06105"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOJ01176.1"
                     /translation="MANTNLTTALWITLIGIILVFVGILLLWGMMELLVYLTRKRKKE
                     NEPQDSTFSANDERTLKQKAAAAAVATAIRLQNSTTIPTPLCQQEALTPWQSVQRSHR
                     SLPYSKHTK"
     gene            1646..2077
                     /locus_tag="PLL88_06110"
     CDS             1646..2077
                     /locus_tag="PLL88_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOJ01177.1"
                     /translation="MKIHVKIQNTTYEVVVGDIHARPIQASVNGELFEVWPEESILST
                     LPQKIPYEIGATVQQVKIEAPAQDPAKASGEISAPIPGVIVDILVKAGDAVQYGQELC
                     ILEAMKMKNSIRANREGVIGEILVAGGDQVGHGQTLIRFKD"
     gene            2094..3221
                     /locus_tag="PLL88_06115"
     CDS             2094..3221
                     /locus_tag="PLL88_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOJ01178.1"
                     /translation="MDLQKILSIFTFDIGHFSWKNIVMILVGLILIYLAIKKEYEPVL
                     LLPIGFGCILANLGMAATADGTGFLSALYRAGIQTELFPLLIFIGVGGMIDFSPLLAQ
                     PKMALLGAAGQFGIFATLILAILLGFDLKQAASIGSIGAIDGPTAIFVSGKLAPELLA
                     PIAVAAYSYMSLVPIIQRPVMKLLTTRKERLIRMEYAPRTVSKQALIIFPIAVTVVVS
                     LIAPEAAPLIAALMLGNLLKESGVVDRLNKSAQNEIINIATLFLGLTIGGTMQAESFL
                     QVTTLKVLGLGLIAFILDTVMGLLFGKIMAFFSGGKINPLIGACGISAFPMAGRLSAK
                     MAQDEDFDNFILMHAMGANTAGQLASVIAGGLLLTLVNLLG"
     gene            complement(3258..3944)
                     /locus_tag="PLL88_06120"
     CDS             complement(3258..3944)
                     /locus_tag="PLL88_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ01179.1"
                     /translation="MKLSKPLILIVEGKRSDRSSFSSGLIKKDYDVHSVPSGNDAISF
                     LEQEKPQVVIVDASSLRTAGKRICSALRKEIPEIPVILIVDAGTDIARIKDANEVLVF
                     PFTLQKLLNRIKPFVQRDDLNILEVGPIKLDLTQRWVYCNGKNERLTPRLFIIMKTLM
                     QKPGKLIKREELFKTLWETEYVGDMRSLDVHISWLRKAVEKDPRKPEFIKTRRGVGYY
                     LDLSEKPKKK"
     gene            4144..4770
                     /locus_tag="PLL88_06125"
     CDS             4144..4770
                     /locus_tag="PLL88_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOJ01180.1"
                     /translation="MVDIDLIEATIDSIRMSLTSQQRIIILKDANNERYLPIWIGPYE
                     SESITIALQEIEISRPQTHDLIKSAIDELGGSINRIIISELKDDIFYGIVEIVQKGKK
                     ILLDTRPSDALALAVRTHVPIFISEQIMKSAGITPVEEEYVSTFRQDIHAPSSEPPTS
                     SEKGEKDVDRLSVFEEYLQKLDQNGEKDDNEDDEKDEDDDSSENMSAI"
     gene            4772..6166
                     /gene="dnaB"
                     /locus_tag="PLL88_06130"
     CDS             4772..6166
                     /gene="dnaB"
                     /locus_tag="PLL88_06130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOJ01181.1"
                     /translation="MNEFSDLQTNEEQTIHSQPHNKQAEEAVLGAVLINPDSFFDVAQ
                     VLEADNFYIIRNRWIWEVFLYLHENQIPIDILTVSEELEKRNQLGEIGGQAYLLSLIN
                     QTPSSLNAEAYAHIVEETSIRRRLLGTANEMAKLAYDQEKGINNVLDEAEREVFNLSE
                     RRIRRDLQPIQIVLSQYYDRVTELSERNGEMLGVPTGLVDLDKVLGGMQKSDLLIIAG
                     RPATGKTGFLLTVAKNAAQKHNKHVAFFSLEMSNEQLVQRLISQETRINAQRLRVGGL
                     EEDEWPLFTHAIEVLGNTKIFLDDTPALTPVQMRTKCRRLHLEYHLDLIVIDYLQLMS
                     GDTRNDNRVQEVSYISRNLKTLARELNVPVLAAAQLSRAVEQRTDKRPMLSDLRESGS
                     LEQDADIVMFIHRPDITDKDISKQNSAEIIVAKHRNGPTHAGIQIVFLNEIARFENAA
                     QVEKEPRRKVVPKE"
     gene            6163..6828
                     /locus_tag="PLL88_06135"
     CDS             6163..6828
                     /locus_tag="PLL88_06135"
                     /inference="COORDINATES: protein motif:HMM:NF018914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HOJ01182.1"
                     /translation="MSKHVLDLKDVHLSARFLLDTVGEIQDAPQLKVLLFAIWLAQQS
                     GDEANPLTLQDFYSYPEFINQLGDYPEQIQEGIISAIDANLKTGTFIGNTLEEIQKGK
                     TFYLNTVRGLQASQLQNQNAPSRSVNLVHTDVFNLYEENIGPLTPLIADALKDAEKEY
                     PREWLEEAIRIAVVNNVRKWRYIQAILTNWKEEGRNGRDQRHAEEDYKQYTKGKYGKF
                     IKN"
     gene            6806..>7958
                     /locus_tag="PLL88_06140"
     CDS             6806..>7958
                     /locus_tag="PLL88_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ01183.1"
                     /translation="MANSSKIEKKAALPGDPNCPICHGIGFVRYDLPVNDPNFGKMEI
                     CVCRQEQVRKLKEEHLYNVSNVESFRSMTFDSFNIHGRTNEKDANKSLEVAFKSSKAY
                     ASQPNGWLFLTGKFGCGKTHLAAAIANEIISNGMQTLFLTVPDLLDWLRYAFSGEEAS
                     FETRFSEIRDIPFLVLDDLGTQNSTPWAEEKLFQIINHRYVNRLPTVITSNLDIAEIN
                     ERISSRINDRELVMKISIIAPDYRDPFSEHKDSPISTLAYLSDHRTFNNFSARKNEKL
                     PANEQKSLDQAFLAAQRFAENPTGWLVFLGAYGTGKTHLAAAIANYRQTAGDEPIFTV
                     VPDLLDHLRATFNPNSPISYDSVFNQIRNAKLLILDDLGIQNTTPWAKEK"
     assembly_gap    7959..7968
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8155..8844
                     /locus_tag="PLL88_06145"
     CDS             8155..8844
                     /locus_tag="PLL88_06145"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOJ01184.1"
                     /translation="MLSINNELFTRLLLIRHGQTIHNTQLLISGINDIPLTEKGHQEA
                     QLLADRVKSQYKVAALYTSPLQRTVQTAEYLSRALNIPAIPNQDLIEYNFGILEGIKT
                     SSLRQTNPELFRKIDEWCESDFSDCPIRPEVPGSESLESLKKRIRSFSEMILEKHPGE
                     VVAAVSHGGFIKSCLYYHTGGDFSRYIPYWVDNVSISIIDFYKGNPVIRLFNDNSHIK
                     EDLVLGSPRWL"
     gene            complement(8862..>9914)
                     /locus_tag="PLL88_06150"
     CDS             complement(8862..>9914)
                     /locus_tag="PLL88_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOJ01185.1"
                     /translation="GLGAIDEFELNLGLKNPDSGGKTKSGHFSTSASVLEEMRGQHKV
                     VDLILEYRELSKLVSTYLEALPKQVNPRTNKIHTSFSQTGSVTGRLASSEPNLQNIPI
                     RTDLGHQVRKGFIADKGHVLVSVDYSQIELRVVAHMSKDQAMLEAFRKGMDIHAATAA
                     AIYNVPIDKVTKDQRRHAKAINFGLIYGMSAFGLSRTTDLTLAEAENFVKTYFERFPN
                     VKTYLDGIRKQAASAGYVETMLGRRRYFPNLKNPINAQVKAREEREAINAPIQGTAAD
                     ILKIAMIKLEPAIQKAGLHAKMLIQVHDELVLECPENEKQDTIAVVKEIMENACKLDI
                     PLSTDARYGNNWAELK"
     assembly_gap    9915..9924
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9925..11693)
                     /locus_tag="PLL88_06155"
     CDS             complement(<9925..11693)
                     /locus_tag="PLL88_06155"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOJ01186.1"
                     /translation="MPKTIYLLDGHALAYRAFFALMATGSRFQTRTGEPTAGTYGFIN
                     ILLRIIEQDDPEYLAVAFDTGKTFRHDLYKEYKATREKMPDELIPQIERIRTLVDTFN
                     IPRLEMQDYEADDVLGSVANLAVKEGFGVKIITGDRDLLQLVNDRIIVNLTGSKMSEA
                     QDYFREDVKKALGVFPDQVVDYKALLGDTSDNIPGVKGIGEKTAGRLLEEYPHLDDIY
                     ANLNKVTGKTRTLLEEGKEDAYLSFKLAQIKTDLPVAIDFEKARTQEVDIEKVLALLK
                     DLEFRSTIERVKKIFHVVEPISTESTQPDLFSVPAQPINITVDPQYDLHVEMVNSEDS
                     LAELVRNLNKAEYLSLDTETTSTDPMQADLVGISLATEAGKGYYIPVGHKTGEKQLSL
                     DQVRNALGPYFMRESLKKVGHHLKYDCIVLINHGFTVKPLAFDTMIAAWLLEPSSHRL
                     GLKALAEIELGISMTHIEELIGSGKHQLSMAEIPAEKVAPYAAADAEVPLKLMKIFTD
                     RLEKNQLFAIFEKIEMPLVPVLIDMERNGISIDKQFFKEYSQELSARMREIEKEIYQI
                     LGYPININSTQQLSKALFIDLGL"
     gene            11821..13092
                     /locus_tag="PLL88_06160"
     CDS             11821..13092
                     /locus_tag="PLL88_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOJ01187.1"
                     /translation="MTYNKICVLGMGYIGLPTACTFATHGIHVIGVDINQEIVHGLQN
                     GHLHIYEPGLRTLAKSAMDSGNLSISDEPEPADAFIIAVPTPITEEKKADLEAVKSAA
                     RSIIPHVQKGNLVVLESTSPPRTTTDIVAPILEKSGLKAGADFMLAYSPERVLPGKIL
                     EELIQNTRVIGGIDERSAAAGKELYSTFVKGDILVTNATTAEMVKLMENTYRDINIAI
                     ANEFAQLADRFGVDAWEAIQIANRHPRVNILNPGPGVGGHCISVDPWFLVEAAPDISP
                     LVHTAREVNDNQPRFVIENMKKKLGALAHRKVTTLGLSYKANIDDLRESPAVVIVNLL
                     LKEQAAVTVYEPYKPDGFHGAAKTANSMQEALQDCEILLLLVAHQEFTYMNPREIAKL
                     TNARIAVDMCGGWQREPWEEAGFRFYKLGQP"
     gene            13110..14249
                     /gene="wecB"
                     /locus_tag="PLL88_06165"
     CDS             13110..14249
                     /gene="wecB"
                     /locus_tag="PLL88_06165"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOJ01188.1"
                     /translation="MNNKKIKVLSIFGTRPEAVKMAPVVQELENKAGIESIVCVTAQH
                     REMLDQVLNLFRIEPHIDLNLMRQNQTLAELTANIFQNLDPVLERVNPDWILVQGDTT
                     TVMAAALNAFYRRIHIGHVEAGLRTHDKWAPFPEEINRKIAGVVADLHFAPTTFSKKN
                     LLNEGVPERFIKVTGNTVIDALRQIINEPMPPEIQEFLEKKQILSKKKQLIVITAHRR
                     ENFGEPIREICRAIKTIAEENADSLELVYPVHLNPSIQQPAHEILDGIPNVTLFQPIE
                     YLPLLHLMKNAKLVLTDSGGIQEEATGLGLPTLVLREVTERPEGVEAGVLKLVGTNYE
                     TIVRETNLLLSDQKYYNAMAHAANPFGDGHAAERIVNEILNFPLP"
     gene            14336..15274
                     /locus_tag="PLL88_06170"
     CDS             14336..15274
                     /locus_tag="PLL88_06170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ01189.1"
                     /translation="MQQKGIEVHFDPQRQDVAAILVVGGSSHLAQLAFAKKRNIRIVQ
                     RLDGMNWLHKKTRTGIKHYLRAEWNNHVLSTIRRTYADAIVYQSDFTRNWWDQVYDKA
                     PVEDTVIHNGADLTIFSPIGNGTPPADRMRIMVVEGSFYGGHERDLWNAVGFAKGMAE
                     ISGKHIELVIASRAPQRMLAALPAYENVSIRWLGIVPLTDIPELDRSAHIFFPAEINA
                     ACPNTVIEAMGCGLPVVSFATGSLPELVQGDAGRVVPYGADYWSLEPPDFKPLCEAGL
                     EVYQAQSKFRSAARKRAEARFDMHAMAEKYLKILIP"
     gene            complement(15276..15791)
                     /locus_tag="PLL88_06175"
     CDS             complement(15276..15791)
                     /locus_tag="PLL88_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOJ01190.1"
                     /translation="MRQEVLTWNDIDLLIDHLVPQFDVIFDAMLLITRGGIIPGGLIA
                     EALDIEEILTASVDFPAETRDGQQQDVQHKFLALPQFIQFPDASLLCEKQVLIVDDVW
                     GSGRTISVVKNRVRGAYGIPYTCVLHFNPHRNLFGTEKPDYYAAITDAYITYPWEIDM
                     GKSRMLPFNCK"
     gene            15907..17199
                     /locus_tag="PLL88_06180"
     CDS             15907..17199
                     /locus_tag="PLL88_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOJ01191.1"
                     /translation="MFDLTLKNLARIQEHKIHSLDGAPIGILPFYHGLNLANIPASIC
                     RWFQIPALQDSALDLPDPPISLDSIKNIMLLVIDGLAFEQMQNWLEKGIQKKRSTPCW
                     QNIREMGIFSPLTSVVPSTTANALTSLWTGKMPAEHGVIGYELFLKEFGITVNMILQT
                     QVHPVPANESESIPWDLSKFLPVPQFGAHLKKFGIHPFAFQHEMICDSGLSRMLLPEV
                     EKIPFTTPQDMLQKVNDLLECQHASRKYLYLYWSDIDTLSHHYGPNSRKTRHAWQWFA
                     HVLENFLCERSRRSTYDTLFLITSDHGQIPTHIHPLYEVRNDQEFMSHLEIPPSGESR
                     LPYLFIKAGMEKSFREYVEQRWRGEFSLIPAQQLMKWGVFGKEIMTPDIQNRLGNFVA
                     FPTGNAYWWWAHKENQLLGRHGGFSPQEMITPLLILPA"
     gene            complement(17246..>18524)
                     /locus_tag="PLL88_06185"
     CDS             complement(17246..>18524)
                     /locus_tag="PLL88_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01192.1"
                     /translation="DDDVFLSADQCEMNGSVNGNLVAACRHVIVNGSVAGDAFLFAET
                     VSISDTSVIDGNLFAFGANVEINGNVSGSTASAAASVLLGSDAVVGRNLYFAGFQGKL
                     ERGASVGMDIFGGANQIVMNGTVARDLNADVNAFELRGGIGRNASIQLGTGNEDIDVT
                     LYSSPWANYYPASLPTGIRFYEGASVGNNLSYTSVTNIDSQLQDFVQGKIVFNQVVST
                     DSTSKNTRAFSWNRWFADDYSRFRIGTAFSGLISYFALGALALWLFKKHMAKVQATGK
                     AFPGKAFGWGFLVILVGMLSILLVPVTFILLGVFLGVISLGGLLFTWYGVIGLTIALA
                     FALFFLVVFTLSKVVASYVMGAWLMQDVFKTRAQNHWVDLLVGVVFYVILRAIPYVGW
                     LVGLAASLYGTGTLFIAFTKVEKEKESKIVDSK"
BASE COUNT         5000 a         4664 c         4307 g         4533 t
ORIGIN      
        1 atttgtctat gcgcaagatt tcaccgtgaa ggggggagcg ctgggtgaaa tgcaaagttc
       61 caagatctgc cgcgtcatgg atctggcata tgaaaacggc gccccgatca tcgggctgat
      121 cgattctggc ggcgcgcgca ttcaggaagg tgtgtatagc ctgggtggct atggcgagat
      181 tttccgcagg aattcacaat actccggcat catcccacaa ataagcatca ttctgggtcc
      241 atgcgcgggc ggcgccgcgt attcccccgc cctgactgat ctcatcatca tggtcgagaa
      301 acaatcttat atgttcctca ccggtcccaa tgtgattaag acagtgaccg gcgaagaagt
      361 tgattttgaa gagttgggcg gcgcaaatgt tcatctttcc accagcggta cagccagcct
      421 gaccgctaaa acggaaagcg atgcattgcg gttatgccgg accatcctta actacctgcc
      481 agccaactat gcggaaaacc ctccttttgt cgccccttca gatgatgcga atcgcgtaga
      541 tagggaattg aatgccatcg tgccgcttga ttcatccgtg ccctattcca tgcacgacgt
      601 gatcaatcgc attgtggacc gcagctcatt tcttgaactg caagccactt ttgcccccaa
      661 cgcgattatc gggttcgcca gaatggatgg aggtgcggtg ggtatcgtgg cgcaagaacc
      721 cagcatcatc gctggcgtca tagacatcaa cgcctccgac aagatctgcc gcttcgtgca
      781 gttctgtgat tgttttaacc tgccgatcat cacttttgtt gattcacccg gttttttacc
      841 aggcgtgaat caagaacacg gagggatcat acgccacggc gcaaaagttc tattcgccta
      901 ttccacttcc tcggttccca agatcagcgt ggtaacccgc aaggcatatg gaggggcgta
      961 tattgtgatg agcagcaaat atctgggcac cgatttcact tttgcctggc caactgcgga
     1021 aatagccgtg atgggtccag aaggcgccgc taacatcctc tacgcgaacc agatcaagaa
     1081 cgcagaagat ccagctaaag agagactcgc ggtgaccgaa ttgtataaag agaaatttct
     1141 gaacccctat gcagccgccc atgccggaca tattgatgac gtgatcgaac caagccagac
     1201 cagaccaatc atcatttctg ccttgaagac actgcaccag aaacaggtgc aacatcccat
     1261 gcgcaaacat gggaacgctc cggtataaga ggagaagtgt tcatggcaaa tacaaattta
     1321 acaacagcgc tatggatcac cctcatcggg attattctgg tctttgtggg cattcttttg
     1381 ttgtggggaa tgatggaatt actggtatat ctcacacgaa agaggaagaa agaaaatgaa
     1441 ccacaagatt ccaccttctc tgccaacgat gaaagaacat tgaaacagaa agcggcggca
     1501 gcggcagtgg cgactgccat tcgcttgcaa aattccacca ccatcccaac ccccctctgc
     1561 cagcaagaag cgctcactcc ctggcaaagc gtacagcgca gtcaccgcag tctgccatat
     1621 tcaaaacaca caaagtgagg acggaatgaa aatccatgtg aaaatccaga acactactta
     1681 tgaagttgtt gtgggcgata tccatgccag accgattcaa gccagcgtga acggcgaact
     1741 attcgaggtt tggcccgaag agagcattct gtccaccctg ccacaaaaaa ttccctatga
     1801 aattggagca acggttcagc aagtcaagat cgaagcacct gctcaggacc ccgctaaagc
     1861 tagtggtgaa atatcagcac ccatccctgg cgtgatcgtg gatattctgg tcaaagcggg
     1921 tgacgcggtt caatacggtc aggaactgtg cattctggaa gccatgaaaa tgaaaaattc
     1981 catccgcgct aaccgagagg gagttatcgg tgagatcctg gttgcaggtg gtgatcaggt
     2041 aggtcatgga caaacgttga tacgcttcaa agactgagga tgatgaggaa accatggact
     2101 tgcaaaaaat cctgagcatt ttcacctttg atattggaca ttttagctgg aagaatattg
     2161 tcatgatcct ggtgggattg atccttatct atcttgccat taaaaaggag tacgagccag
     2221 ttctgttgtt gccgatcggg tttggttgca ttctggcaaa cctgggcatg gcagccacag
     2281 ccgatggaac agggtttctc tcggccttat atagagccgg gatacaaaca gaattattcc
     2341 ctttgctcat tttcatcggc gtgggtggga tgatcgattt cagcccgctg ctggcacagc
     2401 ccaagatggc attgcttggg gcagccggtc aatttggcat ttttgccaca cttatcctgg
     2461 cgatactgct gggttttgat ctgaaacagg cggcatccat tggctccatc ggcgccatcg
     2521 atggtccaac ggcaatattt gtttcaggga agctcgcccc cgaacttctg gcgcccattg
     2581 ctgtggctgc ctattcttat atgagcctgg ttcccatcat ccaacgcccg gtgatgaagc
     2641 tgctcaccac tcgcaaagaa cgcctgatcc gaatggagta tgcaccccgc acagtatcca
     2701 aacaagccct gatcattttc ccgatcgcgg tcacggtggt ggtcagtttg atcgcgccag
     2761 aagctgcacc actgatcgca gccttgatgc tggggaatct gttaaaggaa tcaggcgttg
     2821 tagatcgcct gaacaaatca gcgcaaaatg agatcatcaa catcgccacg ctttttctgg
     2881 gattaaccat tggcggtacc atgcaggcag aaagttttct gcaggtaacc actttgaaag
     2941 tactgggatt gggtttgatc gccttcatcc tggataccgt tatgggattg ctttttggaa
     3001 agatcatggc tttcttttcc ggaggaaaga tcaaccccct gatcggtgcc tgcgggatca
     3061 gtgccttccc tatggcagga cggctctcgg caaagatggc tcaggacgag gattttgata
     3121 actttatcct gatgcatgcc atgggtgcca acacagccgg gcaactggca agcgtgatcg
     3181 ccggtggact gctgctgact ttggtaaatc tacttgggta gagtggatac taaaaagagg
     3241 ttttgaacct tcttttttta tttctttttt ggtttttccg atagatccag gtaatatccc
     3301 accccccgcc gggtcttaat gaattcgggt ttgcgcggat ctttctccac ggctttcctc
     3361 aaccaactga tgtgaacatc cagggaacgc atatccccta catattctgt ttcccagagc
     3421 gttttaaaca gttcttctcg tttgataagt tttccgggtt tttgcatgag tgttttcatg
     3481 atgataaaca ggcggggggt caagcgctcg tttttcccat tgcaataaac ccatctctgc
     3541 gtcagatcca gtttaatggg accaacctca aggatattaa gatcatcgcg ctgtacaaaa
     3601 ggcttaatcc gattcaacag cttctgcaat gtaaaaggaa aaaccaaaac ttcattcgca
     3661 tctttgatcc tggcaatatc cgtgcccgca tcaacgatca agattaccgg aatttccggg
     3721 atttctttgc gcaacgccga gcaaatgcgc tttcctgcgg tgcgcaaaga agacgcatca
     3781 acaatcacca cctgcggctt ttcttgctcg agaaaactta tcgcatcatt accgctagga
     3841 accgaatgca catcataatc tttcttgatc agcccactcg aaaaagaaga cctatccgaa
     3901 cgttttcctt cgacaatcag tataagtggt tttgataatt tcatagagtg tgttgtcgcc
     3961 ttgtttatgt gctcataaaa gtctctggaa ccaatttgcc agttccctag cggcggcggt
     4021 gatggtggta atcattacaa aaaattatac aacatcctta aaataaatac atggttttgt
     4081 tgattataca ttttttgacg gcttacatgt taaaatagca gaaagatttc ccaggagtat
     4141 tgtatggttg atatagattt aattgaggca accattgaca gcatacgcat gagcttgacc
     4201 agccaacaac gaatcattat tctgaaggat gcgaataatg aacgatacct gccgatctgg
     4261 atcggaccct acgagtcaga atccattacc attgcgttac aggaaattga aatctcacgc
     4321 cctcagaccc atgatcttat taaaagtgcc attgatgaac tgggagggtc gatcaaccgt
     4381 atcatcatca gcgaattgaa agatgacatt ttctacggga tcgtggagat cgttcaaaag
     4441 ggaaaaaaga tcctgctcga taccagaccc tctgacgcgc tggcgctggc agtgaggacc
     4501 cacgtgccca ttttcatttc agaacagatt atgaaatcag ccggcatcac acccgtagaa
     4561 gaagaatatg tatcgacttt caggcaagac atccatgcgc cttcatccga acctcctact
     4621 tcttctgaaa aaggagaaaa ggacgttgat cggctatccg tcttcgaaga atatttacaa
     4681 aaactcgatc agaatggcga aaaggacgat aatgaggatg atgaaaagga tgaagatgat
     4741 gattcttcag aaaatatgag tgccatttga tatgaacgag ttcagcgatc tacaaacgaa
     4801 cgaagagcag accatccatt cgcaaccgca caacaagcag gcagaagaag cagtgctggg
     4861 tgccgtgctt atcaatcccg attcattctt tgacgtggca caggttttgg aagcggataa
     4921 cttttacatc attcgcaacc ggtggatatg ggaagttttt ctctacctgc atgaaaacca
     4981 gatcccgatc gacatcctga cagtcagtga ggaactagaa aaacgcaatc aattagggga
     5041 gatcggtgga caagcctatt tattgtccct tatcaaccag acccccagtt cgttgaacgc
     5101 agaagcgtat gcacacatcg tcgaagagac atccatccga cgccgcttgt tgggaactgc
     5161 caacgaaatg gcaaaactgg cttacgacca ggagaaaggg atcaacaacg ttctggacga
     5221 ggctgaacga gaagtcttca atctgagcga acgacgtatc cggcgcgatc tgcaacccat
     5281 tcagattgtg cttagccaat attatgaccg ggttactgaa ctttcagaac ggaatggaga
     5341 aatgctgggc gttcccaccg gactggtgga tctggataaa gtgctgggtg gcatgcaaaa
     5401 atcagatcta ctcatcatcg ccggtcgccc cgccaccggg aagaccggct ttcttctgac
     5461 ggttgccaag aacgccgcgc aaaagcacaa caaacatgtg gcgttctttt ccctcgagat
     5521 gtcaaatgag caattagttc agcgcttgat ctctcaggaa actcgcatca acgcacaacg
     5581 gctcagggtc ggagggttgg aagaagacga gtggccgctg ttcacccacg ctattgaagt
     5641 gctgggcaat acaaaaatat tcctggacga cacgccagcc cttaccccgg tgcagatgcg
     5701 cacaaaatgc cgcagattgc atcttgaata tcatctcgat ctgatcgtca tagattattt
     5761 gcagttgatg tcaggcgata cgcgcaatga taaccgggtt caggaagttt cgtatatttc
     5821 ccgcaatctg aaaacccttg cccgtgaatt gaatgtgcct gtgctggcag ccgcgcagct
     5881 ttcacgcgcc gtggagcaac gcaccgataa gcggccgatg ctctccgatc tgcgcgaatc
     5941 tggcagtttg gagcaggatg cagatatcgt catgttcatc catcgacccg atatcaccga
     6001 taaagacatc agcaaacaaa actcggctga gatcatcgtc gccaaacaca gaaacggtcc
     6061 cacccacgcc ggtatccaga tcgtattctt gaatgagatc gcccgctttg aaaatgcagc
     6121 gcaagttgaa aaagaacccc gcagaaaagt ggttcccaaa gaatgagcaa acatgttttg
     6181 gatctcaagg acgttcatct ctccgctcgt tttcttctcg acacggtcgg agaaattcag
     6241 gatgccccgc aacttaaagt ccttctattt gccatctggt tagcgcaaca aagcggagat
     6301 gaagccaacc cgcttacctt gcaagatttt tattcgtatc ctgaattcat caatcaactg
     6361 ggcgactatc ccgaacagat ccaggaagga ataatttctg caatagatgc taacctgaaa
     6421 actggcactt tcatcggaaa tacgcttgaa gaaattcaaa aagggaaaac tttttacctg
     6481 aacaccgttc gcggattgca ggcatcgcaa ctgcagaacc agaacgctcc gtctcgttct
     6541 gtcaatctag tccatacgga tgttttcaat ctttatgaag aaaacattgg tccgctcaca
     6601 ccgcttatcg cagacgcctt gaaagatgct gaaaaagagt accccagaga atggctggaa
     6661 gaagccatac gcatcgccgt ggtgaacaat gtgcgcaaat ggcgctatat tcaagccatc
     6721 ttgaccaatt ggaaagaaga aggtcgcaat ggaagagatc aaagacacgc tgaagaggat
     6781 tacaaacaat ataccaaagg caaatatggc aaattcatca aaaattgaaa agaaagctgc
     6841 tttacccggt gaccccaatt gtcccatctg ccacggtatt ggttttgtgc gttatgacct
     6901 gccagtcaat gacccgaact tcgggaagat ggagatctgc gtctgtcgcc aggagcaggt
     6961 gagaaagctc aaagaagaac acctgtacaa tgtcagcaat gtcgaatcat ttcgctctat
     7021 gactttcgat tccttcaata ttcatgggag gacaaatgaa aaggacgcta acaaaagttt
     7081 agaagtagct tttaaaagct cgaaagccta cgcatcacaa ccaaacggat ggttgttctt
     7141 gaccgggaaa ttcggctgcg ggaaaaccca tttggcggct gccattgcca acgaaatcat
     7201 cagcaatggc atgcaaaccc tgttcctcac agtgcccgac ctgctggact ggttacgcta
     7261 cgccttcagc ggcgaagagg cgtcatttga aacacgtttt tcagagatca gagatattcc
     7321 cttcctggta ttggacgatc tgggaacgca aaattccact ccctgggctg aagaaaaact
     7381 gttccagatc atcaaccatc gttatgtgaa ccgcttgccc accgtcatca cttccaatct
     7441 ggatatcgca gaaatcaatg aacgcatcag ttcacgcatc aatgaccgcg agttggtcat
     7501 gaagattagc atcatcgccc ctgattaccg cgatcctttt tctgagcata aagattctcc
     7561 catttccacc ctcgcttatc tcagcgatca ccgcacattc aacaattttt ctgcgcggaa
     7621 gaatgaaaag ttgcccgcca atgaacaaaa gagtttggat caagctttcc tggcagcgca
     7681 gcgttttgcg gaaaacccga ctggatggct ggtattcctg ggcgcatacg gaacagggaa
     7741 aacccacctg gcggctgcca ttgccaatta ccggcagacg gctggcgatg aaccgatatt
     7801 taccgtagta cccgatctgc ttgaccactt aagggctacc tttaatccca attctccgat
     7861 atcctatgat tccgtcttca accagatccg caatgccaaa ttgttaattc tggatgatct
     7921 gggaatacaa aataccactc cctgggcaaa agaaaagcnn nnnnnnnntg aattttcgct
     7981 atgaaacaaa agctccgacc gtcattacca cttcagtcaa attggaagat atggaccccc
     8041 gcatcagaag tcgtatgctg gatacgaggg tctgtgaaat tttttcactc gaggttcgac
     8101 cttttgagaa tcttcaataa ttcactccac tacaaaagga attagcttaa aactatgtta
     8161 tcgatcaaca atgaactctt cacgcgcctg ctgcttatcc gtcatggaca aaccatccat
     8221 aatacccagc ttctcatctc aggaatcaac gatatccctc tcaccgaaaa ggggcatcaa
     8281 gaagcacaat tgttagctga cagagtcaaa tcccaatata aggtagccgc tctgtatacc
     8341 agccctctcc agcgaaccgt gcaaaccgca gaatatcttt cacgggcatt gaatattccg
     8401 gcaatcccca accaggactt gatcgaatac aattttggca ttcttgaagg aatcaaaacc
     8461 tcttcactgc gccaaaccaa cccagagtta ttcagaaaaa ttgacgaatg gtgtgaatcg
     8521 gacttttccg attgccccat tagacccgaa gttccgggaa gcgagtccct tgaaagtttg
     8581 aaaaaaagaa ttcgatcttt cagcgagatg atcttagaaa agcacccggg agaagtcgtg
     8641 gctgcggtgt cacatggcgg ttttattaag agctgcctct actaccacac gggtggtgat
     8701 ttctcgcgct atatccccta ctgggtagac aacgtgtcaa tctcaatcat cgatttttac
     8761 aagggaaatc cggttatccg tctgttcaac gataacagtc acatcaagga agacctagtg
     8821 ttaggaagcc cgcgctggct ctgatgcaaa gaaccaagtt cttacttcaa ctcagcccaa
     8881 ttgttgccat agcgcgcatc ggtgcttagc ggaatgtcca gtttacaggc attttccatg
     8941 atttctttga ccacggcaat ggtgtcctgt ttttcatttt ccggacattc caacaccagt
     9001 tcatcatgca cctgaatcag cattttggca tgcagaccgg ctttctgaat agccggctct
     9061 aatttgatca tggcaatctt gagaatatcc gctgcggttc cttgaatggg ggcgttgatg
     9121 gcttctcgtt cttcacgcgc tttcacctgc gcattgatgg gattctttaa attcggaaaa
     9181 tatctgcgcc tgcccagcat ggtttccaca taccctgcac tggcagcctg tttgcggatg
     9241 ccatccagat aggtttttac attcggaaac cgttcaaaat aggttttcac gaaattctcc
     9301 gcctccgcca gggtgagatc ggtggttcgg cttaacccga atgcgctcat accgtagatc
     9361 aatccaaaat tgatggcttt ggcgtgccgg cgctggtcct tggtcacctt atcaattggc
     9421 acattataga tcgccgccgc agtcgcagca tggatgtcca tgcctttgcg gaacgcttcc
     9481 agcatggctt gatcttttga catatgcgct accacacgca gttcgatctg ggaataatcc
     9541 accgaaacca gcacatgccc tttatccgca atgaagccct tgcgcacctg atgacccaaa
     9601 tctgtgcgaa tagggatatt ctgcaaattc ggttcggagg atgccagcct gcccgtgact
     9661 gaaccggtct ggctgaatga ggtgtgaatt ttgttagtgc gggggtttac ctgtttaggc
     9721 agtgcttcca gataggtaga gaccagtttg gaaagctcac gatattccag gatcaggtcc
     9781 acaaccttat gctgcccgcg catttcctcc aggacggatg cagaggtgga aaaatgtcct
     9841 gacttggtct tgccgcccga atcggggttt ttcaagccca ggttcagttc aaactcatca
     9901 atggcaccga gtccnnnnnn nnnnttcaag cccaggtcaa tgaacaaggc tttggaaagc
     9961 tgctgggtgg aattgatatt gatcggataa cccagaatct gatagatctc tttttctatc
    10021 tcgcgcattc tggcgctcaa ctcctgagaa tattccttga aaaactgttt gtcgatcgaa
    10081 ataccgttcc tttccatatc gatcaatacc ggcaccagag gcatttcgat cttttcaaag
    10141 atagcgaaca attggttctt ctcaagccgg tccgtgaaga ttttcatcag ctttaaaggg
    10201 acttcagcgt cagcggctgc atacggagcc actttttccg cggggatttc cgccatagaa
    10261 agttgatgtt ttccactgcc aatcaattcc tcaatgtggg tcatgcttat ccctaactca
    10321 atctctgcca gagctttcaa tcccagacga tgtgaggaag gctcaagcaa ccaggctgcg
    10381 atcatggtgt cgaaggcaag cggtttgacg gtgaacccat ggttgatgag aacaatgcag
    10441 tcgtatttca gatggtgacc tacttttttc aaagattcac gcatgaaata aggacccagt
    10501 gcgtttcgca cctgatcaag ggacagttgt ttttcacccg tcttatgacc gaccggtatg
    10561 taatatcctt tgcccgcttc agttgccagc gaaattccca ccagatctgc ctgcattgga
    10621 tccgttgaag ttgtctctgt gtccaacgag agatattcgg ctttattcag gttccgcacg
    10681 agttcagcca gtgaatcttc tgaattcacc atctctacgt gcagatcata ctgcggatca
    10741 acagtgatat taatgggctg cgccggaacg ctgaataggt ctggttgtgt gctttcagtg
    10801 ctaattggtt caacaacatg gaaaattttt ttaacgcgtt ctatggtaga tcgaaattca
    10861 aggtctttca acaatgccag aactttctca atatccactt cctgggtgcg cgctttttca
    10921 aaatcaatag ccacaggaag gtcggtcttg atttgggcaa gtttaaagct gaggtaagca
    10981 tcttctttcc cttcttcaag aagggttctg gttttgccgg tgaccttatt gagattggca
    11041 tagatatcat ccaggtgcgg gtactcctcg agaagccgac cggcggtttt ttctccgatt
    11101 ccctttacac cggggatatt atctgaagtg tctcccagaa gcgctttgta atccaccacc
    11161 tgatctggaa agacacccaa tgctttcttc acatcttcac gaaaataatc ttgcgcttcc
    11221 gacattttcg aacctgtcag gttcacaata atgcgatcat tcacaagctg cagcagatcg
    11281 cggtcgccgg tgatgatctt cacaccaaat ccttctttga ctgccagatt ggcgacagat
    11341 cctagcacat catccgcttc atagtcttgc atttcgagtc gggggatgtt gaaagtatca
    11401 accaaagtac gaatgcgttc gatctgcgga atcaactcat ccggcatttt ctcacgggtc
    11461 gccttatatt ctttatacag gtcatgccgg aaagtttttc cggtgtcgaa agccactgcc
    11521 aggtattccg ggtcatcttg ctcaatgatg cgaagcagaa tattgatgaa accgtaggtg
    11581 ccggcagtgg gttcccctgt gcgggtttga aagcgcgatc cggtagccat cagagcaaag
    11641 aacgcccgat aagccagcgc atgtccatcc aacagataga tcgttttcgg catactgtcc
    11701 tcttttctga gagttaatga agtttatcat gccagaaaat ccttttcaac aattcccgca
    11761 aaagccaatt tttttgaatt atgggataat aggggcattg tccacgtcga ggagctgact
    11821 ttgacatata acaagatctg tgttttaggg atgggatata tcgggctgcc cactgcctgt
    11881 acttttgcca cacatggcat ccatgtcatc ggtgtcgaca tcaaccagga gatcgtgcac
    11941 ggcttgcaga atgggcattt gcacatctat gagcccggat tgcgcacgct ggcaaaatct
    12001 gccatggatt caggaaacct ctccatcagc gacgaacctg aaccggctga cgcctttatc
    12061 atcgccgtcc ctacgcccat aaccgaagaa aaaaaagctg atctggaagc ggtgaagtcc
    12121 gctgctcgct cgatcattcc tcatgtacaa aaaggcaatt tggtcgtcct ggaatcaaca
    12181 tccccgcccc gtacaacaac ggatattgtc gcccccattc ttgaaaagag cgggttgaaa
    12241 gcgggtgccg atttcatgct ggcttattca cccgaacgtg tgctgcccgg gaagattctc
    12301 gaagagctca tccagaacac ccgtgtgatc ggtggaatcg acgaacgatc ggctgccgcc
    12361 ggaaaagagc tttacagcac atttgtcaaa ggtgatattc tcgtaaccaa tgccaccacc
    12421 gcggaaatgg tcaaattgat ggaaaacacc taccgcgata tcaacatcgc gattgccaat
    12481 gaattcgcgc agttggcaga tcgctttggc gtggatgcct gggaagccat ccagatcgcc
    12541 aaccgccacc ccagggtgaa cattctgaac cctggtccgg gagtgggcgg tcattgcatc
    12601 agtgtggatc cctggtttct ggtggaagcg gcgcccgata tctcacccct tgttcatacg
    12661 gcacgggagg tcaatgacaa ccaaccccgt tttgtcattg agaacatgaa gaaaaaacta
    12721 ggcgcgctcg cccatcgcaa ggtcaccacg ctggggcttt cttacaaagc caacatcgat
    12781 gacctgcgtg aatcgcccgc tgttgtgatc gtcaatttac tcctgaaaga gcaagccgca
    12841 gttaccgttt atgaaccata caaaccagac ggatttcatg gggcagccaa aacagccaat
    12901 tctatgcaag aagccttgca agattgcgaa attttgcttc tgctggtagc tcaccaggaa
    12961 ttcacctata tgaatcccag ggaaattgcc aaactgacaa atgcccgcat cgcagtggat
    13021 atgtgcggag gctggcagcg ggagccatgg gaagaagccg gttttcggtt ttataaactg
    13081 ggtcaaccat aaactactat aatcaaagta tgaacaataa aaagatcaaa gtgctgtcca
    13141 tttttggaac gcgacctgaa gcggtcaaga tggctccggt agtacaggag ctggagaaca
    13201 aagcgggcat cgaatccatc gtatgcgtta cagcgcagca tcgcgaaatg cttgaccagg
    13261 ttctcaatct tttccgcata gaaccacaca tcgatttgaa tctcatgcgc cagaaccaga
    13321 ccctggctga actcactgcc aatatctttc aaaacctcga tccggtcctg gaacgtgtca
    13381 atcctgattg gatactggtg caaggggata ccaccactgt catggcagca gctttgaacg
    13441 ctttttaccg gcgcatccat atcgggcatg tggaagccgg gctgcgcacg catgataaat
    13501 gggcaccttt tccagaagag atcaatcgca agatcgctgg tgtggtggca gatctgcact
    13561 tcgcgccaac cacgttcagc aaaaagaact tgctgaacga aggcgtacca gagcgcttta
    13621 tcaaagtgac cggcaatacg gtcatcgatg cgctgcggca gatcatcaac gagcctatgc
    13681 cacctgaaat tcaggaattc cttgaaaaaa agcaaatttt gagcaaaaag aaacaactca
    13741 tcgtcattac tgcacatcgc agagagaatt ttggtgaacc aatccgtgag atttgccggg
    13801 ctatcaaaac cattgcagag gaaaatgccg attcgctgga attggtctac cccgttcatt
    13861 tgaatccaag cattcagcaa ccggctcatg agattctgga tggaattccg aacgttacgc
    13921 tttttcaacc gatcgaatat ctgccactgc tacacctcat gaaaaatgcc aaactggtgt
    13981 taaccgattc gggaggaata caggaagaag cgactggctt gggattacca accctggtgc
    14041 tgcgggaagt gaccgagcga ccagaaggcg tggaagccgg cgtgttaaag ctggtcggga
    14101 caaattacga aacgatcgtt cgtgaaacca acctgttgct gtctgatcaa aaatattaca
    14161 atgccatggc acatgccgcc aatccgtttg gtgatggtca tgctgccgag cgtattgtga
    14221 atgaaatcct gaatttcccc ttaccttgac catgaaaact ggcagcattt gcctatttcc
    14281 acgattagag cataccggcg gtccttcttc gtttcagagg aaactgatca tctgcttgca
    14341 gcagaaagga attgaagttc atttcgaccc gcagcggcaa gatgtggcag ccattctggt
    14401 ggttgggggc agcagccatc tcgctcaact tgccttcgca aagaagagaa acatccgtat
    14461 cgttcagcgc ctggatggca tgaactggct gcataaaaag accagaactg gcataaagca
    14521 ctacctgcgc gccgagtgga acaatcacgt cctctccacc atccgccgaa cctatgccga
    14581 tgcaatcgtc taccaaagcg atttcacgcg caactggtgg gatcaggtat atgacaaagc
    14641 gcccgttgaa gataccgtga ttcataacgg cgcagacctg acaatattct cacccatagg
    14701 gaatggcacc ccaccagccg atcggatgcg tatcatggtc gtggaaggca gcttctatgg
    14761 aggtcatgaa cgcgatctat ggaacgcagt gggatttgcc aaagggatgg cagagataag
    14821 tggaaagcac atcgaactgg tcatcgccag cagagccccc caaagaatgc ttgcagcgct
    14881 tcctgcgtat gaaaacgtgt caatccgctg gttgggaatt gtgccgttga ccgatattcc
    14941 ggaattggat cgcagcgcgc acatcttctt cccggctgag atcaacgcag cctgtcccaa
    15001 cacggtcatc gaagccatgg gctgtggttt acctgtggtc agctttgcaa ccggttctct
    15061 gcctgaattg gtacagggtg atgctggcag ggtcgtaccg tacggtgccg attattggtc
    15121 actggaacca cccgatttca aacccctctg cgaggcgggg cttgaagtgt atcaagccca
    15181 atcaaagttt cgttcggctg ccagaaaacg cgccgaagcc cgttttgaca tgcacgccat
    15241 ggctgagaaa tatctaaaaa tcttgatccc atagatcatt tgcaattgaa aggcagcata
    15301 cgtgatttcc ccatatctat ctcccaggga tacgtgatat aagcatcggt gatcgctgcg
    15361 taataatctg gcttttccgt cccgaaaaga ttgcggtgag ggttgaaatg cagcacacag
    15421 gtataaggaa tgccataagc tcccctgacg cggtttttga caacactgat cgtccgccct
    15481 gaaccccaca cgtcgtctac aatcaagacc tgtttttcac acaacagaga ggcatcaggg
    15541 aattggataa attgtgggag tgccaggaat ttatgctgta cgtcttgctg ttgcccatcc
    15601 cgagtttcag caggaaagtc aaccgatgca gttaatatct cttcgatatc caacgcttct
    15661 gcaatcaatc cccccggaat gatccccccg cgggtgatca gaagcatggc atcaaagatc
    15721 acatcaaatt gtggaaccag gtgatcgatc aacaggtcaa tatcattcca tgtcagaact
    15781 tcttggcgca tacagtgtca tcccgaagag agataattca ttatatcaat aatcccaagc
    15841 agcaaaaata ccgcgcatgc agtatagtta tatccaacag ttgcccaatc ataggatgct
    15901 tctattttgt tcgatcttac tttaaagaat ttagcccgta ttcaagaaca caagatccat
    15961 agtctggatg gagcgccaat agggatcctt cctttttacc atgggttgaa cttggcgaac
    16021 attcctgcca gcatctgccg ttggtttcag atcccggcgt tgcaagactc cgcattggat
    16081 ctccctgacc cgcccatctc gctggattcc ataaagaata tcatgctgtt agtgatcgat
    16141 ggcttggcat tcgaacaaat gcagaactgg ctggaaaaag ggatccaaaa aaaacggtca
    16201 acaccctgtt ggcaaaacat aagggaaatg ggaatttttt cacctctcac cagcgtggtg
    16261 cccagcacca ctgccaatgc actgaccagt ttatggaccg gaaaaatgcc cgcagagcac
    16321 ggcgtgatcg gttatgaact atttctcaaa gaattcggca tcacagtgaa catgatcctg
    16381 caaacacaag ttcatcctgt gccggcgaat gaatccgaat ccatcccctg ggacctcagc
    16441 aaattcctac cggttcccca atttggtgct catttaaaga aattcggaat tcaccctttc
    16501 gcatttcaac acgaaatgat ctgtgattcg ggtctatcac gcatgctcct gcctgaggtt
    16561 gaaaaaattc cctttaccac tccgcaggat atgttgcaaa aagtgaatga cctcttagag
    16621 tgtcagcacg catctcgaaa gtatctctat ctgtattgga gtgatattga taccctctct
    16681 catcactatg gacccaatag ccgcaagacc cgtcatgcat ggcagtggtt tgcgcatgta
    16741 ctggaaaatt ttctatgcga gcgatcacgc cgctctacgt acgatacgct atttttaatc
    16801 acctccgatc acggacagat tccaacgcac atacacccgc tctatgaagt gagaaacgac
    16861 caggaattca tgagccatct ggaaattcca cctagcggtg aaagccgtct cccctacctg
    16921 ttcataaaag cgggaatgga gaagtcattc agggaatacg tcgagcagcg ctggagaggg
    16981 gaattcagcc tgatccctgc gcagcaattg atgaaatggg gcgtctttgg aaaagaaatc
    17041 atgacacctg atatccagaa ccgcttgggg aacttcgtcg cttttccgac aggaaatgca
    17101 tattggtggt gggcgcataa agagaaccag ctattgggca ggcatggcgg attctcgcca
    17161 caggaaatga tcacgcctct tctcatccta cctgcatagc ctatagaaaa aagaacccgc
    17221 tcggctcgag cgggttcttt cgtaactatt tactgtcaac tattttggat tctttttctt
    17281 tttctacttt ggtgaacgcg ataaagagcg tgcctgttcc atataaactg gctgccagac
    17341 cgaccagcca gcccacgtag ggtatcgctc gcaagatcac gtagaatacc acaccaacca
    17401 gaagatcaac ccaatggttc tgtgcccggg tcttgaaaac atcttgcatc agccaggcgc
    17461 ccatcacata actggccaca actttgctga gggtaaagac caccaggaag aacagagcga
    17521 aagccaacgc gatagttaat ccaatcacgc cataccatgt gaacagcaac ccacccaatg
    17581 agatcacacc caggaaaaca cccaatagga tgaaagtcac cggtacaagg agaatactca
    17641 acattccaac caggatgacc aaaaagcccc aaccaaatgc ttttccaggg aatgccttgc
    17701 cggttgcctg cacctttgcc atgtgctttt tgaacagcca taaagccaaa gcacccaagg
    17761 caaaataact gatcaacccg ctgaatgcag tgccgattcg aaaacgagag tagtcatcag
    17821 caaaccatct attccatgaa aaggctctgg tatttttaga ggtactatcc gttgaaacga
    17881 cttggttaaa aacgatcttg ccctgcacaa aatcctgaag ctgcgaatcg atatttgtaa
    17941 cagatgtata ggagagattg ttaccaacgg aagcaccttc atagaagcgt atacccgttg
    18001 gcagactcgc aggataataa tttgcccagg gtgatgaata caacgtaaca tcgatgtctt
    18061 cgtttccagt gcccagttga atggatgcat tccttccaat gccaccgcgc agttcaaacg
    18121 cgtttacgtc tgcattgagg tcgcgcgcga cagtgccgtt catgacaatc tgattagccc
    18181 cgccaaagat atccatgccc accgaggcgc ctctttccag ttttccctgg aatccagcaa
    18241 agtagaggtt ccttcctacc actgcatcgg aaccaagcag aacagatgca gccgcactgg
    18301 ctgtggaccc tgaaacattg ccgtttattt caacattagc accgaaggca aacaggtttc
    18361 catcaatgac agatgtatcg ctgattgata cggtttcagc gaagaggaac gcatcgcctg
    18421 ccacgctacc gttaacaatg acatgtcggc aggcagcaac caggtttcca ttgaccgacc
    18481 cgttcatttc acactggtca gcggatagaa atacatcatc atcg
//
LOCUS       DAOSDS010000040        18502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1230_length_18502_cov_5.312354, whole genome shotgun sequence.
ACCESSION   DAOSDS010000040 DAOSDS010000000
VERSION     DAOSDS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18502
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1230_length_18502_cov_5.312354"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLL88_06190"
     CDS             <1..513
                     /locus_tag="PLL88_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ01193.1"
                     /translation="MVVLGAAYGLGGDLTNVQPGLDFFAQLIDKGILNGVKPSTAAIQ
                     KGECPISLFWDFDGLSAKEENPEMNLEVIIPSEGTVAGMYIQFVTAGAPHANVAKLMI
                     ELEFSDEGQLAYANGFVHPMRSSVVLPDELKAKFPPDEAYEVVTFPTDYEALDAAGIA
                     ISEGWELIAQ"
     gene            643..1461
                     /locus_tag="PLL88_06195"
     CDS             643..1461
                     /locus_tag="PLL88_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01194.1"
                     /translation="MSSTGISVRLNRLFSDGKNAVVVAVDHGLYFGPLAGMIDLPAVI
                     QKVARADAVLMSPGMPAHCQNVFSKRGSPACIIRLNWGSNYAAMWNYKHSHSVPMIPV
                     ADAVLEGADLVIGSLTLRNPDEMEDAHNVEVFSQCVAQKRALGIPLIGELYPTGGDDR
                     QPEDLQEDIYIGCRIIAELGADLVKTFYTGEGFGKIVDATPVPVLALGAKKLPKASDA
                     LRLAAQAVEAGGRGVVFGRNVIQAADPDRFLDALEEVVKDFQAPDKVAEKYKLD"
     gene            1463..2986
                     /locus_tag="PLL88_06200"
     CDS             1463..2986
                     /locus_tag="PLL88_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ01195.1"
                     /translation="MKFLLGIDVGTTSLKAGLFSLDGFCLGIQREEYSLDTPSVERVQ
                     LNPIFYWEACVRTIHALLENVGCDPKEIASIAVSSQGETLITLNVDGEPIYPAIIWLD
                     NRASKQAERLSEKFQARVYQKTGIPEIIPTWTACKVLWLKENKPIVFQSAAKFLLVQD
                     YIIYRLTGDFISDGSISCTTLYFDIVQKTWWEEMLNEIGITKDQLPNLCEAGSVVGHL
                     QESAARELGLPIHTKIVNGGMDQAAGAIGAGNIQTGIISETTGSALAMQVTVEHSDID
                     AQKLIPVYIHSVPNHYLLVPVCPTAGMALKWFRDQFASEEIHKAETQQKDSYDLLTDL
                     IASVPAGCDGLLMLPHLMGAFSPEINSVARGSFTGFTLSHTRAHFARAVMEGVAFMLK
                     RNVEYVEKTGIPIHEIISTGGGSRSHLWNQIKANVCDKSVITLQNEETALLGDIILAG
                     TAINVFSSIQEGCQRMITFKETYSPNNESGSYIKPYKNYCILERTLFEFFSNVYPKE"
     gene            3018..4406
                     /locus_tag="PLL88_06205"
     CDS             3018..4406
                     /locus_tag="PLL88_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01196.1"
                     /translation="MKNKPKVGVLVMALLEDDYNKTAHVRPMAQKVADHIGEIINKYA
                     DAVCPPLVEEEHQAEAAAQLFNSQDVDLIIAIEIAYTKGIVPTRCFLNTTAPILVWNT
                     QQIESLPEDADFDLIMVNSGMAGVPEMTSALLRIGRPFWMITSMMDDPSGHQKIADYI
                     QAAGVIHDLKRARIAIFGHAFEGMTDLMIDQLSLRQHVGPVCWPVEPEKVAVAMGEIS
                     DSDVKALMAKESGRYRINIAEKQFERSCRLALALEQVTREGKFDAAASFDQVWLTDPR
                     VGIIPSYGTGRLCELGIPCSTEADITTLTAMLILQGFAGKATFLENYVIDFKRDAIIL
                     SHDGHGNPAMAAAPSDVTLKHSIYYEGVQGFGAGFEFAYAAGPVTNLSLVPVQGKWRL
                     IVSEGESIAITPRPVAAPQMLFKPNHLPITDWCTRWCEAGAPHHQALAFGHLGEKIKI
                     YAQMQGLQLIEV"
     gene            4439..5221
                     /locus_tag="PLL88_06210"
     CDS             4439..5221
                     /locus_tag="PLL88_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ01197.1"
                     /translation="MNEINTAERRSQIAQMVIEKGQVYVSDLVEKFKVSEVSIRRDLT
                     LLEKARRLKRVHGGAIPIPGNFRTDSFEEKEKLHNEAKERIGQFAANLITQGNIILLD
                     SGTTTLQIVRTIPSFLRVSNMITMVTNSMPLTQEVLTWPSPNLTILGGIYLPDYQATV
                     GPQTLNQLQELTADIAFLGTDGLTLEGGATTANVLMAEVDRMMADQAQKTVLVTDSSK
                     FGRKGFVPVKKVSSYHMIITDVDAPEDMVKAIRDMGVEVVLV"
     gene            5265..5978
                     /locus_tag="PLL88_06215"
     CDS             5265..5978
                     /locus_tag="PLL88_06215"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003302.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOJ01198.1"
                     /translation="MDTGITLEPPFFEIGPKAYIYGAKALQLAMHADQMSEKYNVRII
                     FTPQYVDIPLIASNTKRILVFAQHMDSLEVGRGIGSVLPEALKEAGADGFLLNHAEKR
                     VSLDEIAKTISRGNKSGLLSMVCADSPIQAIEIAKLNPDMILAEPPDLIGVGARQEAD
                     QEEINRINHKVWQINPDIKILHGAGIKNGKDIYNIIKRGAQGSGSTSGILLADNPFQM
                     LEEMISATRKAWNETHSSN"
     gene            5993..6487
                     /locus_tag="PLL88_06220"
     CDS             5993..6487
                     /locus_tag="PLL88_06220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ01199.1"
                     /translation="MSVYHQSIQVQSKMVPTFHDVTEGAKNALKNSGIKNGIMVVYSQ
                     HTTCSVMIQEESHDENFFGTKSIMQDLLNIFEEIVPTCRSEGQYLHPGPAHIADATRR
                     KELPCWSLNTDAHLRSIIMGRSESIPIIDGKLELGEFGRIYFADFDQVRARERTVRFQ
                     IIGE"
     gene            7119..7445
                     /locus_tag="PLL88_06225"
     CDS             7119..7445
                     /locus_tag="PLL88_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01200.1"
                     /translation="MTKRRCRDLFLYSGETDIILDLAKLAAQIGVITIAITSSRNDNQ
                     IAKISDLVIYFSAYSGIDIPLAYTDTRIAMLNVVDILYTFSIQKYENIEDVQKLNIIA
                     NSIPKK"
     gene            complement(7487..7996)
                     /locus_tag="PLL88_06230"
     CDS             complement(7487..7996)
                     /locus_tag="PLL88_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HOJ01201.1"
                     /translation="MSDLTDYRKMKDNFYLSDARSPLTEEQKQTFQGLQYYPENPALR
                     FMLEIEEFSNKELVSIQTSKGDVRTYERYGKINFAVDGKPVALTVYKADVDFFLPFID
                     SMAGKETYPAGRYLEIEPVENGKFLVDFNMAYNPYCAYNDRWSCPLTPAENRLKVAIR
                     AGEKLYEAH"
     gene            8161..8622
                     /locus_tag="PLL88_06235"
     CDS             8161..8622
                     /locus_tag="PLL88_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HOJ01202.1"
                     /translation="MKKKVFVFLVVVIFMLSAACNSKVNAADTTATTSSPAAVVTNFY
                     SEYLSYFEDPETNAFRSPLASRIYANRNYFTQESIDKIDGIMASFSGGGAYDPILCAQ
                     NIPDYITVDTPYVSAGRVMFMIGDNFEDHHFSVHLTKESDGWKIDAINCDF"
     gene            8686..8973
                     /locus_tag="PLL88_06240"
     CDS             8686..8973
                     /locus_tag="PLL88_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01203.1"
                     /translation="MKVLALEVEKQSAALSDFDPLLIEEAQKVWEFQQRDIIREVYFN
                     AVSHCAVIILEAKSQDQAKEILAQLPLVKEGLITFNLIPLTPYDGYARLFQ"
     gene            complement(8977..10470)
                     /gene="glpK"
                     /locus_tag="PLL88_06245"
     CDS             complement(8977..10470)
                     /gene="glpK"
                     /locus_tag="PLL88_06245"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOJ01204.1"
                     /translation="MADYIASIDQGTTSTRCMIFNGKGEVMGSHQLEHTQIYPQAGWV
                     EHNPNEIWARTQDVIVEAMRKAGARPEDIAAIGVTNQRETTVVWDKTTGKIYYNAIVW
                     QDTRTDKIIQELGKAGGQNRFQQKVGLPLATYFSGPKIKWILDSVEGARQAAEEGRAI
                     FGNMDTWLIWNLTGGVHVTDVTNASRTMLMDLKTLQWDVEVCRVMDIPMQMLPQICPS
                     SQIYGYTKPDGPFKGAIPVTGDLGDQQAATVGQACFDVGEAKNTYGTGCFMILNTGHE
                     LVFSKNGLLTTLCYQFKDEKPVYALEGSIAITGALVQWLRDNLKMIGSATEIEDLAKT
                     VDDNGGMYLVPAFSGLFAPYWRSDARGVIVGLTRYINRGHFARAALEATAFQTREVLE
                     AMKADSNVDLLVLKVDGGMVQNDLLMQIQADVLQVPVIRPKVAETTALGAAYAAGYAV
                     DFWKDLNEMRNNWKKDKVWTPKPLSPLYSEKFKEWKKAVTRTFDWVS"
     gene            10681..11667
                     /locus_tag="PLL88_06250"
     CDS             10681..11667
                     /locus_tag="PLL88_06250"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOJ01205.1"
                     /translation="MEKTEKINNAIRAARMYYYQGMTSGAIAREMNLSRSSISRLISF
                     AREKGYVDIRILDPIEQPLVLEKEILDYYPVQKVHVVPVPEIVGEAEWLERSAQFAAK
                     YLNTLFDSNMILGVAWGTTLSAISRYLQPKATYNSQIVQLNGAGNTKSMGIEYASEII
                     MRFATSFQARAHLFPVPTFFDYASTKNALWKERSIQRILQLQQEADLLLFSIGAVNAG
                     VPSHVYAGGYLEESDLQAIRHNQIVGDIATVFFREDGSFENIALNQRTSGPDLTLFRE
                     KHSLCVVSGLAKVRGLHAALQGQFITELIVDEPTARALSEHRKVKGKSFEES"
     gene            11704..13200
                     /locus_tag="PLL88_06255"
     CDS             11704..13200
                     /locus_tag="PLL88_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ01206.1"
                     /translation="MNNNRNYDVIIIGGGVIGCMLARFLSRYRLDILVIEKETDIGSV
                     TSAANTAIVHPGYDAVPGSLKAQLNVAANPLWDQLANELHFAFERRGDYVVAIGKEEL
                     PKLDELLQRGKQNGVIGMRTVSAEEMREREPNINPAVSGALWATSGGICDPFGVTIAA
                     AENAIANGVVILRETSFEDFMWDGKRITGVKTNRGDFTCRWAVNAAGLFSDTVMHKAG
                     IRPAFTITPRKGEYFVLDRTEFTVNNVLFPVPTNISKGILVTTTVHGNTILGPNAQNI
                     EDKENKENTKDGMNEVFEGAKKLVPGVNPRHIIAVFAGLRAAGNAPCTTPGVDYNHDF
                     IVEIPGEVQGFFNLGGIESPGLTAAPAIAQYVVELLKDAGEKLQERKDWNPIRPARPQ
                     FRHLTHQQRDVLIKKDARYGRVVCRCENVTEGEIVAEIHAPVPALTYDAIKRRTWLGT
                     GRCQGGFDMPRVTEILARELGISPLEVSKKNGASKFLYRPTKEEGGAQ"
     gene            13197..14474
                     /locus_tag="PLL88_06260"
     CDS             13197..14474
                     /locus_tag="PLL88_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ01207.1"
                     /translation="MKIENCDIAVIGSGPGGLAAAMAARQNGAKKVLLIERDREPGGI
                     LLQCIHNGFGVEIFKKDLPGPAYAQHFINEAQSAGVEFLLDTMVLEITKDKRIFASNK
                     KEGMFEIQAKAIILAMGCRERTRAQVRLPGARPAGVYTAGTAQRWVNVEGFMPGKQVV
                     ILGSGDIGMIMARRLTFEGAKVERVLEIMPYLSGLMRNYVQCLQDFGIPLLMQHTVKR
                     IIGNERVEAVETIAVDDRWQEVAGSEEIIPCDTLLLSVGLIPENELSRQVGIQLDPLT
                     SGPFVDNHFQTSIEGFFAAGNVVHVYDLVDWVTGAGLRAGESAALYAHGKLHGNKKVT
                     IQHGQNVHHVIPHSLDISSLAFHEEQLQMRVTRPLEHPVKVQVMNGNEVIASKALPYA
                     RPGEMVTISLKDKHMEPLQNAGQLVVRVVEREE"
     gene            14477..14869
                     /locus_tag="PLL88_06265"
     CDS             14477..14869
                     /locus_tag="PLL88_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HOJ01208.1"
                     /translation="MNTNLPTQQIICVACPKGCRLNIQRDGDQVLVSNQGCKRGVEYA
                     HGEVKDPRRMVATTVRIEGARHPLLPVYTASPFPKGRIFELLAELRNLQIQAPVKEGQ
                     IILENALDTGIPVLASRDMEPLIPDGSR"
     gene            15010..15336
                     /locus_tag="PLL88_06270"
     CDS             15010..15336
                     /locus_tag="PLL88_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01209.1"
                     /translation="MGNGVEGERTANSGNAVISASSAGACATVQPDDDQALVLNQVAS
                     AVQAVRKGGRKVPHRMPASTVGIERAKHLSACLHRNALKKVFSFLPAKTDRKRKRVFQ
                     SFRRVK"
     gene            15616..16833
                     /locus_tag="PLL88_06275"
     CDS             15616..16833
                     /locus_tag="PLL88_06275"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ01210.1"
                     /translation="MKTFRIFVVFTILALAFAGCKAQVEVPTGDVTIQLWTQEGESDN
                     AYQYVVSLTDAFTALYPNITFEVVQKDTETLREDFQTASLAGAAPDLLWTVSDHAGPF
                     TTAGLIQPVDEYFDMDLYVPSVVMNDSTWAVPISSGNHLMLIYNKDLVAEAPADTDEL
                     IAKAEELTQGDTYGLVYNATEPFWLVPWLGGFGGSVFAEDGVTPTLNTDTMVNTLQFL
                     YDLKFTYQVTPESIDYDTADTLFKEGKAAMIINGDWSLGAYKELFGDKLGVAPLPKVT
                     ATGIYPAAYTSGKYFMLPKDLAGDKLDAAIKFIEFATNEENQLNQLSTLSRLPGLKSV
                     LSNEAITSDPILAGSALAMSYGTPMPTVTEMRCSWDAMKPEMNLVLAGSETPADAAAA
                     MQAATEACILQLE"
     gene            16932..17936
                     /locus_tag="PLL88_06280"
     CDS             16932..17936
                     /locus_tag="PLL88_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ01211.1"
                     /translation="MFNFDAIIKSLSEVGTTILAVLLIVVLLEVLLYLILVKWLKVKN
                     SLVYMLISPAILGLLVLQVYPLLWEGYISMTNMSLSHFFNFDFIGLKNYVRVFTEPVL
                     KQVRFFPLFWRTILWTAINVVFHVTGGMILALLLNREMKLRGFYRMLIIIPWALPQVV
                     AAMAWRGEFNFEYGWINIMLKNIGLQPVQWLTSPIPNFAAMCIVNIWLGIPFMTVILL
                     GGLQSISGEYYEAAEMDGASNWQKFWKITMPLMQPIMSPAVILGTVWTFNTFNISFFI
                     NQYELESSDILVTALFRAAFQYNRYGFSAAFAFIIFFILLIYSTIYARSTGALKGVKD
                     "
     gene            17936..>18502
                     /locus_tag="PLL88_06285"
     CDS             17936..>18502
                     /locus_tag="PLL88_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ01212.1"
                     /translation="MTTQHATINPSTLEQKQPKKRKSNFFRANRGDSKFKRLIIHIIL
                     ILYCLISVYPLLRIFSVSLRPGNRVLSTDLSIIPPDATFENYEKVIFEQGLFQWIWNS
                     LAVTITTAIIGVIIAATSAYAFSRYKFKGRTTLLIYLLTTQMIPTAMLMVPLYILAIN
                     LNLIGTYRGLVIAYSVTSVPFSIWILKGY"
BASE COUNT         5034 a         4438 c         4413 g         4617 t
ORIGIN      
        1 atggtggttt taggtgccgc atacggtctg ggcggtgatt tgactaatgt acaacctggt
       61 ttggactttt ttgcacaact aatcgataag ggcattttaa atggagtaaa accctccact
      121 gcagctattc aaaaaggcga atgtccgatc tcgttgtttt gggatttcga tgggttgagc
      181 gcgaaagaag aaaatccaga gatgaacttg gaagtcatta tccccagcga aggaactgtg
      241 gctggcatgt atatccaatt cgtgacggct ggagcccctc atgcgaatgt tgccaaacta
      301 atgatcgaat tagaattttc cgatgagggt cagttagctt acgcgaacgg atttgtgcat
      361 cctatgcgat caagcgtcgt tctccccgat gagctgaaag caaagttccc gcctgatgaa
      421 gcctacgagg ttgtaacttt cccaactgat tatgaagctt tagatgctgc tggcattgcc
      481 atttctgagg gttgggagtt gatcgctcaa taaatatcaa ggcaaggatg gggcagggaa
      541 accctgctcc atcccttcaa gataagattg tatcttaaaa gcgcgtgaag aaatcatcta
      601 atcattttat gaaagaaact tttgttgtat aaggagaaaa gaatgtcatc cacaggaatt
      661 tcagtaagat tgaacaggtt gttttcagat ggcaagaacg cagtagtagt tgcagtagac
      721 catggtttat attttggacc actggcaggc atgattgatc ttcccgcagt aatccaaaaa
      781 gtcgcgaggg cggatgctgt tttgatgtca cccggaatgc ccgcccactg ccaaaatgtt
      841 ttttcaaaac gtgggtcacc cgcttgcatc attcgcctta attgggggtc gaattacgca
      901 gccatgtgga actataaaca ttctcacagc gttcccatga tccccgttgc agatgcagta
      961 ttagaaggag ccgaccttgt aatcggtagc ttaactttgc gaaacccgga tgaaatggaa
     1021 gacgcacaca atgtggaagt tttttcacaa tgtgtagctc aaaaacgcgc attgggcatt
     1081 cctttaattg gagagcttta tcctactggg ggagatgatc ggcaacctga agatctacag
     1141 gaagatattt atattggatg tcgtatcatc gcggaattgg gtgctgatct ggtgaaaact
     1201 ttctatacag gtgaaggttt tggaaagata gtcgatgcta ccccagtgcc agtgttggct
     1261 ttaggagcga agaagttacc gaaagccagt gacgccctca ggttggcagc acaggctgtt
     1321 gaagcggggg gacgcggagt cgtattcggt cgaaatgtga tccaagcagc agatcccgat
     1381 cgcttcctag acgcgttgga agaggttgta aaagactttc aggcacctga taaagtagca
     1441 gaaaaatata aattggattg agatgaaatt tttattggga attgacgttg gaactacttc
     1501 tctaaaggcc ggactttttt ctctggatgg gttttgctta ggcattcaac gcgaggagta
     1561 ttccctcgat accccctcag tggaaagagt tcaacttaat cccatttttt attgggaagc
     1621 ttgtgtacga acaatacatg ctttactaga gaacgttggt tgtgatccaa aggaaattgc
     1681 atcaattgca gtctccagcc agggcgaaac gctcatcacg ctcaacgttg atggagaacc
     1741 aatctacccg gcaataatat ggttggataa ccgggctagc aaacaagcag aaaggttatc
     1801 agaaaagttt caggctcggg tttaccaaaa gaccggtata cccgaaatca tccctacctg
     1861 gactgcctgc aaagtattgt ggttaaaaga aaataaacca attgtctttc aaagtgctgc
     1921 gaaatttttg ctggttcagg attatataat ttatcgttta accggggatt tcattagtga
     1981 tggatccatc tcctgcacca cactatattt tgacattgta cagaaaacgt ggtgggagga
     2041 gatgctcaac gaaattggga tcacgaaaga ccaactgcca aatctgtgcg aggcaggatc
     2101 agttgttggt catcttcaag aaagcgccgc tcgggagttg gggcttccca tccataccaa
     2161 aattgtaaat ggaggaatgg accaagcagc cggcgctatt ggcgcgggga atattcagac
     2221 aggtatcatt tccgaaacaa ccggatctgc tttagcaatg caagtgacag tagaacattc
     2281 agatatcgat gcgcagaaat taattcccgt atatatccat agtgttccaa atcactatct
     2341 acttgtccct gtttgcccta cagcaggaat ggctttgaaa tggttcagag atcaatttgc
     2401 cagtgaagaa atccacaaag cagagaccca acaaaaagat agctatgatc tactcacaga
     2461 ccttattgca tcagtccccg ctggttgtga tggcttactc atgttacccc atttgatggg
     2521 cgctttcagc ccagagatca attctgtcgc acgcggttct tttacaggtt ttacactaag
     2581 ccatacacga gcacattttg cgcgggcagt tatggaaggt gtggctttta tgttaaaacg
     2641 taatgttgaa tacgttgaaa agacaggtat tcctatccat gaaataattt ctacaggagg
     2701 aggatcacgc agtcacttat ggaatcaaat caaagctaat gtttgtgaca aatccgtgat
     2761 aactttgcaa aatgaagaga ctgccctgct aggagacatt attttagcag gcaccgcgat
     2821 aaatgttttc tcttcaattc aagaaggttg ccaaagaatg atcactttta aggaaactta
     2881 cagccccaat aatgaaagcg gatcatatat caaaccttac aagaattatt gcattctgga
     2941 aagaacatta tttgagtttt tttcgaatgt ttaccccaaa gagtaagaaa tataagagaa
     3001 aaggagaaat aagaatcatg aaaaataaac caaaagtcgg cgttctggtg atggctttgc
     3061 tcgaggatga ctacaacaaa accgctcatg ttcgtcctat ggcacaaaaa gtagctgatc
     3121 atattggtga gataatcaat aaatatgctg acgcagtttg tccaccattg gtagaggaag
     3181 agcatcaagc agaagcagcc gcccaacttt tcaactcaca agacgtggat cttatcattg
     3241 caattgaaat cgcctacacc aaggggattg taccaacccg ttgttttctc aatactaccg
     3301 ctccaatttt agtttggaat acacagcaaa ttgagtcgtt gcccgaggat gctgattttg
     3361 atttgatcat ggtcaattct ggcatggctg gagtgcctga gatgacctcc gcccttctac
     3421 gcatcggcag accattttgg atgattacct ctatgatgga tgatccatca ggacatcaaa
     3481 aaatagccga ttacattcaa gccgcaggag ttatacacga cttaaaaaga gcacggattg
     3541 ctatctttgg gcatgcattt gaaggaatga ctgacctgat gattgatcag ctaagtctgc
     3601 ggcaacatgt aggaccagtt tgttggcctg tagagccaga aaaagtggca gtagcaatgg
     3661 gcgaaatttc tgatagcgac gtaaaggcat tgatggcgaa ggaatctggt cggtatcgta
     3721 tcaatattgc cgaaaaacaa tttgaacgat cctgtcgact tgctttagcg ctagagcaag
     3781 ttactcgtga aggcaaattc gatgcagcgg catctttcga ccaagtctgg ctgactgatc
     3841 cccgcgtcgg aattatccca tcttatggaa ctggacgcct atgcgaatta ggaattccat
     3901 gcagcactga agcggatatc accactttaa ctgccatgct gatcctgcag ggttttgccg
     3961 ggaaggcaac ctttctagag aattatgtta tagactttaa acgagatgcc atcattctct
     4021 cacatgatgg gcatggaaac cctgcgatgg ctgcagcccc atctgatgtg acactgaaac
     4081 acagtattta ctacgagggc gttcaaggat ttggagcagg ttttgaattt gcttatgccg
     4141 ccggaccggt caccaatctg tcacttgttc ctgtacaagg gaagtggcgt ctgatcgtct
     4201 cagaaggaga atcaattgca attacgcccc gccccgttgc agcgccacag atgcttttca
     4261 agccaaatca tctgccgatt actgattggt gcacaagatg gtgcgaagct ggtgcaccac
     4321 accaccaagc attagcattt ggacacctcg gagagaaaat aaaaatttat gcacagatgc
     4381 aagggttaca attaatcgaa gtctagattt gtgaaaatta ggatctatgg tgatggttat
     4441 gaatgagata aataccgccg aacgcaggtc acaaattgcc caaatggtaa ttgaaaaggg
     4501 acaagtttat gtctcagatc tggtagaaaa gtttaaagta tctgaagttt ccattcgccg
     4561 cgatttaaca cttctggaga aagccagacg tctaaaacgc gtgcatggag gtgccattcc
     4621 aatcccaggc aattttcgca cggattcttt tgaagaaaaa gaaaaattgc ataatgaagc
     4681 aaaggaacgc attggtcaat tcgccgccaa cctcattacc caaggaaata tcatcttgct
     4741 agattcgggt acaaccaccc ttcagatcgt gcgcacgatc ccgtcatttc tacgagtgag
     4801 caatatgatc accatggtaa ccaactcaat gccgttaacg caagaggtgc tcacctggcc
     4861 atcaccaaat ttaaccatct taggcggtat ttatctccca gactatcagg ccacggttgg
     4921 tccgcaaaca ttgaaccaat tgcaggaatt aacagctgat atcgcatttt taggaacaga
     4981 tggtttaaca ctagagggtg gagcaactac tgccaatgtg cttatggcag aggtcgatcg
     5041 aatgatggca gatcaggcac aaaaaaccgt gctagttaca gatagctcca agtttgggcg
     5101 caaggggttt gtacccgtga aaaaagtctc ctcttatcac atgatcataa ctgatgtgga
     5161 tgctccagaa gacatggtaa aagctatccg tgatatgggg gtagaagtgg tcttagtcta
     5221 aaaaggaacg cttttcatac cacatagaaa gaataatcaa taagatggat acaggaatca
     5281 ccctcgaacc tccctttttt gaaattggtc caaaagcata catttatgga gcaaaagcac
     5341 ttcagcttgc catgcatgca gatcaaatga gtgaaaaata taatgtgcga attattttca
     5401 cgccccaata cgttgatatc ccattgatcg ccagtaatac aaagcgcatc cttgtattcg
     5461 cacaacatat ggattcattg gaggtaggaa gaggaatcgg ttcagtactt ccagaagccc
     5521 ttaaagaagc tggggcagat ggtttcttat taaatcatgc agaaaaaaga gtatccctcg
     5581 atgagatcgc gaaaacgatc agtcgcggaa ataagtcagg attgctttct atggtctgcg
     5641 cagattcacc catccaagca attgaaatag ccaaactcaa tcctgacatg attcttgcag
     5701 aaccgcccga tctcattgga gtcggagcac gacaagaagc agatcaagaa gaaattaatc
     5761 gaataaatca taaagtttgg cagataaatc cagacatcaa gattttacat ggtgctggaa
     5821 ttaaaaatgg aaaagatatc tacaacatca tcaagcgtgg agcacaaggt agcggatcca
     5881 caagtggtat tttactggca gataatccat tccaaatgct agaagaaatg attagcgcaa
     5941 cacgcaaagc atggaatgag acccattcat caaactaaaa gaggtaattc ctatgtctgt
     6001 ttaccatcaa tctatacaag tgcaatcaaa aatggttcca acttttcacg atgtgacaga
     6061 gggtgcaaaa aacgcactca aaaattccgg aatcaaaaat ggcattatgg ttgtttattc
     6121 acaacacaca acttgcagtg ttatgatcca agaagaatca cacgatgaaa atttctttgg
     6181 aacaaaatcc atcatgcaag acctgctaaa tatttttgag gagattgtac ctacttgtcg
     6241 aagcgaaggg caatatttgc atcctggacc agcacatatc gctgatgcta cccgtagaaa
     6301 agaattaccc tgttggagtt taaacaccga tgcccatctt cgctcaatca tcatgggacg
     6361 atcagaatca atcccgataa ttgatgggaa gttagaatta ggagaatttg ggcgcatata
     6421 ttttgccgat tttgaccaag tgcgcgctcg agagagaacg gttcggttcc aaattattgg
     6481 tgaataattt gctctaactt taataagata aaatcaagtg attttgaaaa ttcaaacgaa
     6541 ttcatgaccc ttcttttcga tctcgtcatg aacatggaac ctcgcaaagt catatctcca
     6601 gggtttttat gaaataagtt cttaaccaaa tgcatttatt gtatcctcac tcataaactt
     6661 cggctactcg taaaactatt ctctgtgcag ttaaaaacct aaagcaactg tcatctggcg
     6721 gactaaaatc atctctccag gctcatactc ggcgaggtgg atcgtgggtc aacacgtgga
     6781 agaacagtta caaagtcatc aggtacattg attccgcgga ctagcaggtt gaaatattac
     6841 atcacgccta aataccaacc gtctttatct caagttattt tttacatttt gatatcactc
     6901 ctgttttatc aaactcatca agaagcatga aaaattcaag agatgatgga agaacatccc
     6961 cgctcatcac agacaagaaa tgatttcatt cctcaacgat cacttatttt cacgccagat
     7021 caatagacta agtagctggt gaaatttcaa ccattaatta ccccaaggca tccgcaatca
     7081 gcagacatgt taatcagtta caaaatgtgt aggaattaat gacaaaaagg agatgtcgcg
     7141 atcttttttt atattcgggt gaaaccgata tcatcctcga cctcgccaag ttagccgccc
     7201 agatcggcgt gatcaccatc gccatcacct cttctcgtaa cgacaaccaa atcgctaaga
     7261 tatccgacct ggtcatctat ttttccgctt attcaggtat cgatatcccg ctggcttata
     7321 ctgacacgcg cattgccatg ttgaatgtgg tggatattct ctacaccttc tccatccaaa
     7381 aatatgaaaa catcgaggat gtgcagaaac tgaatatcat cgccaacagc atccccaaaa
     7441 aatagggatt ccactgattc gaaaactttt caataacagg gagcaatcaa tgcgcctcat
     7501 agagcttttc accagctcga atggctactt tcaagcggtt ctctgccggg gtgagcgggc
     7561 aggaccagcg gtcattatag gcgcaatagg ggttataagc catattgaaa tcgaccagaa
     7621 atttgccatt ctcgactggc tcaatttcca aatagcgacc agccgggtag gtttccttgc
     7681 cagccatgct atctataaaa gggagaaaaa agtcgacatc ggctttgtag actgtcagcg
     7741 cgaccggttt tccgtccacc gcaaaattga tcttgccgta ccgctcgtag gttcgcacat
     7801 ctcctttgga agtctggatg gaaaccagct ctttgttcga gaactcttcg atctccagca
     7861 taaagcgaag ggctggattt tccgggtaat actgcaaccc ctggaaggtt tgtttctgtt
     7921 cctcggtgag cggacttcgc gcatcggaga ggtaaaaatt atctttcatc ttgcgataat
     7981 cggtcaaatc actcattttc aacctcttgt cctttatcgg taatcctagc gatcattgta
     8041 gcggttatgt agaagaaaat ccatctgcag atcattaaca ttaaactttc tgaaaatctc
     8101 ttttttttgt ctattaaggt ttatcataca cacctgcaaa tcatctcgta ggagtaattc
     8161 atgaaaaaga aagtttttgt ttttttggtc gttgtaattt ttatgctatc ggctgcatgc
     8221 aattcgaagg tgaacgccgc agacaccaca gccaccacca gttctccggc tgccgtggtg
     8281 acgaattttt attcagaata tttaagctat tttgaagatc ccgaaaccaa cgcctttcgt
     8341 agtccgcttg ccagccgcat ctacgccaac cgcaattatt ttacgcaaga atcgattgat
     8401 aagatcgatg gcatcatggc atccttcagc gggggcggtg cttatgatcc cattctgtgt
     8461 gcacaaaaca tccccgatta catcacggtc gacaccccgt atgtgagtgc cggcagggtc
     8521 atgttcatga tcggagacaa tttcgaagat catcactttt ccgtccatct gaccaaagaa
     8581 agcgacggct ggaagatcga tgcgatcaac tgtgatttct agctcatcta acaggtccct
     8641 acaggaacct gtttttattt agaatagaaa gagcatggag gaaccatgaa agtgctggcg
     8701 ctcgaagttg aaaaacaaag tgccgctctc agcgattttg atccattgct catagaagaa
     8761 gcgcagaaag tgtgggaatt ccagcagagg gatatcatcc gcgaggtcta tttcaatgca
     8821 gtttcgcatt gtgcggtgat cattctggag gcaaaatcgc aagatcaagc caaagagatc
     8881 ctcgctcaac ttccactggt gaaagaaggc ttgatcacct tcaacctgat cccgctaacg
     8941 ccctatgacg ggtatgccag attgttccag tgagattcat gatacccaat caaaggtgcg
     9001 cgtaaccgct ttcttccact ctttgaattt ttctgaatag agcgggctga gcggtttggg
     9061 ggtccaaacc ttgtctttct tccagttatt gcgcatttca ttgagatctt tccagaaatc
     9121 tactgcgtaa ccagcggcat aggcagcgcc caaagcggtt gtttctgcca ctttggggcg
     9181 aatgaccggc acctgcagca cgtctgcctg gatctgcatc agcaagtcat tctgcaccat
     9241 gcctccatcc accttcaata ccagcaggtc cacattggaa tccgccttca tggcttccag
     9301 cacttcgcgg gtttgaaaag cggtggcttc cagcgctgcg cgtgcgaaat gcccgcggtt
     9361 gatatagcgc gtcagcccaa cgatgacacc gcgtgcgtcc gaacgccagt aaggggcaaa
     9421 caacccggaa aaagccggca ccaggtacat gccgccgtta tcatccaccg tttttgccag
     9481 gtcttcgatt tcagtggcac tgccgatcat tttcaggtta tcgcgcagcc actgcaccag
     9541 agcgccggtg atggcgatgg agccctccag ggcatagacc ggtttttcgt ctttgaactg
     9601 gtagcataag gtggtcagca gtccattctt ggaaaacacc agttcatgcc cggtgttgag
     9661 aatcatgaaa cagccggttc cataggtgtt cttggcttct cccacgtcaa aacaagcctg
     9721 ccccacggta gcggcttgtt gatcacccag atctcccgtt acggggatgg cgcctttgaa
     9781 gggaccatca ggtttggtgt atccatagat ttgagaggat gggcaaattt gagggagcat
     9841 ctgcatgggg atatccatga cgcggcaaac ttcgacatcc cactgcaacg tcttgaggtc
     9901 catcagcata gtgcggctgg cattggtcac gtcggtgaca tgcacgccac cggtcaggtt
     9961 ccagattaac caggtatcca tgttgccaaa aatggcacgt ccttcttcgg ctgcctggcg
    10021 cgccccttcc acgctatcca agatccattt gatcttgggt ccggagaaat aggtcgccag
    10081 aggcaacccc actttctgct gaaagcgatt ttgcccgcct gcttttccca attcctgaat
    10141 gattttatcg gtacgggtat cctgccagac gatggcattg tagtagatct tgccggtagt
    10201 cttatcccac accacagtgg tttcgcgctg gttggtcaca ccgatagctg caatatcttc
    10261 cgggcgcgca ccggctttgc gcatggcttc cacgatcaca tcctgggtgc gcgcccagat
    10321 ctcattaggg ttgtgctcta cccaacccgc ctggggatag atttgggtat gctccaactg
    10381 gtggctgccc atcacctccc ctttcccatt gaagatcata cagcgcgtac tggtagtacc
    10441 ctgatcgatg gatgcaatat agtctgccat gtcatcacct catttttcca atggatctgc
    10501 tcgtcccaat cttatcctgt tcaatttccc tgacatcatt gtagaagatt ttttcgtcgt
    10561 tctcaattgt atagcacaaa ggtgcaaaat tgcacgacac ccggcgccat tgaattgaat
    10621 tattttgcat agatgttgca atattgtgca aaatggagta tttttaacca ggagtgtgga
    10681 atggaaaaga cggagaagat caacaacgcc attcgggcag cccgcatgta ttactatcaa
    10741 ggtatgacca gcggcgcgat tgcacgagag atgaacctct cacgctcatc catttcacga
    10801 ttgatcagtt ttgcccgaga gaaggggtat gtggatattc gcatccttga ccccatcgaa
    10861 caacctctgg tgctcgaaaa agaaatcctg gattattatc ccgtccagaa ggtgcatgtg
    10921 gtgcccgtcc cagagatcgt cggagaagcg gaatggttgg agcgttccgc acagttcgcc
    10981 gccaaatacc tgaacacgtt gtttgattcg aatatgatcc tgggggtagc ctggggcacc
    11041 acattgagcg cgatttcccg ctatctgcag ccaaaagcca cctacaacag ccagatcgtg
    11101 cagttgaacg gagcgggcaa caccaagagc atgggcattg aatatgccag tgagatcatc
    11161 atgcgctttg ccaccagttt tcaggcgcgc gctcacctct tccccgtgcc cacctttttt
    11221 gattatgcca gcaccaagaa tgcattgtgg aaagaacgca gcatccagcg catcctgcaa
    11281 ctgcagcagg aggctgacct tctgctgttc agcatcggcg ctgtcaacgc tggggtaccc
    11341 agccacgtgt acgccggagg atacctggaa gaaagcgacc tgcaagccat caggcataac
    11401 cagatcgttg gggatattgc cacggtattt ttccgcgaag acggcagttt tgagaacatc
    11461 gcgctcaatc agcgcaccag cggaccggat ctgacccttt tccgagaaaa gcacagccta
    11521 tgcgtcgttt ccggcctggc gaaggtcagg ggtctgcacg ccgccctgca agggcaattc
    11581 atcacagagc tgatcgtaga tgaacccacc gccagagctt tgagcgaaca ccgcaaagtc
    11641 aaaggaaaaa gttttgaaga atcataaaag aaaggaccca atcgcagcaa aggtgagcct
    11701 tgcatgaaca acaacagaaa ttatgatgtc attatcatcg gaggaggtgt gatcggctgc
    11761 atgttggcgc gctttctctc gcgctacaga ctggatattt tggtcatcga aaaggaaacg
    11821 gatatcggtt cggtgaccag cgctgccaac accgccatcg tgcatccggg gtacgacgcc
    11881 gtccccggtt cgttgaaagc ccaattgaac gtggctgcca accccctgtg ggaccaattg
    11941 gcaaatgaac tacatttcgc tttcgagcgg cgcggagatt acgtggttgc cataggcaaa
    12001 gaagaactgc caaaactgga tgaactcttg cagcgcggaa aacaaaatgg ggtgatcggc
    12061 atgcgcacgg tcagcgcgga agaaatgcgc gagcgcgaac ccaatatcaa tccggctgtg
    12121 agcggagcgc tctgggcaac cagcggcggg atctgtgatc cgttcggggt gaccatcgct
    12181 gccgccgaaa acgcaattgc gaacggcgtg gtcattctga gagagaccag ctttgaggat
    12241 ttcatgtggg atggaaaacg catcacgggc gtcaaaacca atcgcggaga tttcacctgc
    12301 cgctgggcgg tcaacgctgc cggtttgttc tccgacacgg tcatgcacaa agcaggaata
    12361 cgccctgctt ttacgatcac tccccgcaag ggggaatatt tcgtgctgga tcgcacagaa
    12421 ttcacggtga acaacgtgct gttcccggtt cccaccaaca tcagcaaagg cattctggtc
    12481 actaccaccg tacacggcaa caccatcttg ggcccgaacg cacagaacat cgaagataaa
    12541 gaaaacaagg agaacaccaa agacggcatg aacgaagttt tcgaaggcgc aaagaagctg
    12601 gtccccggtg tgaatccgcg ccatatcatc gcagtctttg ccgggctgcg cgccgccggg
    12661 aacgcgccct gcacaacccc cggcgttgat tacaaccatg atttcattgt cgagatcccg
    12721 ggcgaggtgc agggtttctt caatctgggc ggcatcgaat cgcccggctt gaccgctgcg
    12781 cctgccattg cgcagtacgt ggtggaactg ctgaaagacg ccggagaaaa gctgcaagag
    12841 aggaaagatt ggaacccaat acgcccggct cgcccgcagt tcagacacct cacccatcag
    12901 caacgcgatg tgttgatcaa aaaagatgcc cgatacgggc gcgtggtttg tcgttgcgaa
    12961 aatgttactg aaggcgagat cgtagcagag attcacgcac ctgtgcctgc actcacctac
    13021 gatgccatca aacggcgcac ctggttggga accggacgct gtcagggtgg tttcgatatg
    13081 ccgcgggtca ccgagatctt agcccgcgaa ttaggaattt ccccactgga ggtcagcaaa
    13141 aagaatggcg cctcaaagtt cctctatcgc cctaccaaag aagagggagg cgcccaatga
    13201 agattgagaa ttgcgacatt gctgtgatcg gcagcggacc gggaggtttg gcagcagcta
    13261 tggctgccag acaaaacggc gcaaagaagg tgctgcttat cgaacgcgac cgggaaccag
    13321 gcggcattct gttgcagtgc atccataatg gttttggcgt cgagatcttc aaaaaggatt
    13381 tgcccggacc cgcctacgcg caacatttca tcaacgaagc gcagtcagcc ggcgttgagt
    13441 ttctgctcga caccatggtg ttggagatca ccaaagacaa acgcattttc gcgagcaata
    13501 agaaggaagg catgttcgag atccaggcaa aagccatcat cttggctatg ggttgtcgcg
    13561 aacgcacccg cgcccaggtg cgcctgcccg gggcacgccc cgcaggggtg tacacagcgg
    13621 gcaccgcgca acgctgggta aatgtggaag ggttcatgcc cggcaaacag gttgtcatcc
    13681 tcggctctgg agatatcggc atgattatgg cgcgtcgatt gacctttgaa ggggctaaag
    13741 tggaaagggt gttggagatc atgccgtatc tttcaggtct gatgcgcaac tacgtccaat
    13801 gcctgcaaga ctttggcatc ccgcttctga tgcagcacac cgtgaagcgc atcatcggca
    13861 atgagcgtgt ggaagccgtg gagaccatcg ctgtggatga ccgttggcag gaagtggcgg
    13921 gcagcgagga gattattcct tgcgataccc tgctgctctc ggtggggctg atccccgaaa
    13981 atgaactgtc acgccaggtc ggcattcagc ttgacccgct cacttcagga ccattcgtcg
    14041 ataatcattt tcaaaccagc atagaaggct tctttgcagc cggcaatgtg gtgcatgtat
    14101 atgacctggt ggattgggta acgggagccg gtctgcgcgc cggcgagagc gctgcattgt
    14161 acgcccatgg caaattgcat ggaaacaaga aggtcaccat ccagcatggg caaaacgtgc
    14221 accacgtcat tccccattct ctcgacatca gcagcctggc tttccacgaa gagcaattgc
    14281 agatgcgcgt cacccgacct ctggaacacc cggtgaaagt acaggtcatg aatggcaacg
    14341 aagtgatcgc cagcaaagcg ctaccctatg cgcgccctgg cgagatggtg accatttcat
    14401 tgaaggacaa acatatggaa ccacttcaaa atgctggtca actggtggtt cgggtagtgg
    14461 aacgcgagga ataaggatga acacaaattt gccaacccaa cagatcatct gcgtggcttg
    14521 cccaaaaggc tgccgcttga atatccagcg tgacggagat caggtgctgg tgtcaaatca
    14581 gggctgcaag cgtggggtgg aatatgccca tggcgaagta aaagatccgc gccgcatggt
    14641 cgccaccacg gttcgtatcg agggagcaag gcaccccctt ctgcctgttt acactgcctc
    14701 cccttttccc aagggacgca tcttcgaact gctggctgaa ctgcgcaatc tacagattca
    14761 ggcacctgta aaagaaggac agatcattct ggagaatgcg ctggataccg gcatccctgt
    14821 tttagccagc cgcgatatgg aaccgttaat tccagatggg tcaagataag agcggtttcc
    14881 attagccgct cttatccact ctttatgcca actgcattgc cggtctgcac accctgtggt
    14941 tgatatctga caagagtatc aggggttcgc gaaaaattca ttttcgttct acaaaatttt
    15001 catcactgca tgggtaatgg tgttgagggt gaaagaacgg caaactctgg aaatgccgta
    15061 atcagtgctt ctagtgccgg cgcctgcgcc actgttcagc ccgatgacga ccaggcactg
    15121 gttctcaacc aggttgcaag tgcagtgcaa gctgtgcgca agggaggaag gaaagttccg
    15181 caccgcatgc cagccagcac ggtcggcatt gagagagcaa agcacctctc tgcctgttta
    15241 caccggaacg cgctgaaaaa agtgttttca ttcctgcctg caaaaacaga tagaaagaga
    15301 aagagggtct ttcagtcttt tcgaagagtc aaataaatct ttcatagata atgtggaaga
    15361 atgtcataaa tgctaactgt cattcttcca aaaaccgatg tatactgaat tatggtatgg
    15421 ttcgattttg tgcagtattg gcaaataaaa atcgataatt cgctatggtt taacaacccc
    15481 ccacttaaca gtacacagta tatgcattag gttttgttca gtttatatga gcaatattaa
    15541 tgatctttat aaggaggtga gatagggagc aattggtgca cactttaaat caatcaattt
    15601 cttgaggaga aaaatatgaa aacgtttagg atttttgtgg ttttcaccat ccttgcatta
    15661 gccttcgccg gctgcaaggc acaagtagaa gtacccaccg gtgatgtcac catccagcta
    15721 tggacccagg aaggcgaatc tgacaatgcc tatcagtatg tggtcagcct gactgatgct
    15781 ttcaccgcgc tgtatccaaa catcaccttc gaggtagtgc agaaagatac cgaaacactg
    15841 cgcgaagact tccagaccgc cagcctggct ggtgcagctc ctgacttgct gtggacggtc
    15901 agcgaccatg ccggtccttt cacgaccgcc ggcttgatcc agcccgtgga cgaatatttc
    15961 gatatggatc tgtatgttcc cagcgttgtc atgaacgatt caacctgggc agtgccgatc
    16021 tctagcggca accacctgat gctgatctac aacaaggacc ttgtcgcaga agctcccgcc
    16081 gataccgatg aattgatcgc aaaggcagag gagctcaccc agggtgatac ttatggtttg
    16141 gtctacaatg cgaccgaacc cttctggttg gtaccgtggc tgggcggttt tggcggcagc
    16201 gtcttcgcgg aagatggcgt gaccccaacc ctgaacaccg acacgatggt caacaccctg
    16261 caattcctgt atgacctgaa gttcacctac caggtcaccc ccgaatccat tgattatgac
    16321 acggccgaca ccctcttcaa ggaaggaaaa gctgccatga taatcaacgg tgactggtcg
    16381 ctgggtgcat ataaagagct gttcggtgac aaactcggtg tggctcccct gccgaaagta
    16441 accgcaaccg gcatctatcc tgccgcttac acctctggta aatatttcat gctccccaag
    16501 gacctggcag gtgacaaact ggatgcagcc atcaaattca tcgaatttgc aaccaacgaa
    16561 gagaatcaac tgaatcagtt gagcacgctt agcagactgc ccggtttgaa atccgtgtta
    16621 tccaacgaag caatcaccag cgatccgatc ctggctggtt ctgcgttagc gatgagctac
    16681 ggcaccccca tgcccacggt aacggaaatg cgctgcagtt gggacgctat gaaaccagag
    16741 atgaacctgg tgctggctgg cagtgaaaca cccgcagatg cagccgcagc catgcaagcc
    16801 gctactgaag catgcatcct gcaattagag taacgtcttt acagcccaaa gcgatggaac
    16861 cggagatctc cggttccatc cttctcatca cacaagacca ttctttgtaa tcatcaaagg
    16921 aggccttagc aatgtttaat tttgatgcaa taatcaaaag cttatccgaa gtaggcacca
    16981 ccattctggc tgtcctcctc atcgtggtgc ttctagaagt tctcttgtat ctcattcttg
    17041 tcaagtggct aaaagtgaaa aattcactgg tgtatatgct catctcacca gccatcctcg
    17101 gtctgctcgt gctgcaagta tacccgctgc tatgggaagg gtatatttcc atgacgaaca
    17161 tgagtctgag ccatttcttt aactttgatt ttattggtct gaagaattat gtgcgcgtgt
    17221 tcacagaacc ggtcctgaaa caggttaggt tcttcccgct gttctggcgc accatccttt
    17281 ggaccgctat taacgtggtc ttccacgtca caggcggaat gattctggca cttcttttaa
    17341 atcgcgaaat gaagttgcgt ggtttctatc gcatgctgat catcattcct tgggctttgc
    17401 cacaggttgt ggctgccatg gcatggcgtg gtgaattcaa ttttgaatac ggctggatca
    17461 atatcatgtt gaagaacata ggattacagc ccgttcagtg gttaaccagc ccaatcccaa
    17521 acttcgcagc catgtgcatt gtcaatatct ggttgggtat tccatttatg accgtcatct
    17581 tgttgggtgg tttgcagagc atctcgggtg aatactacga ggcggctgaa atggatggtg
    17641 ccagcaactg gcaaaagttc tggaagatca ctatgccgct catgcagcct attatgtctc
    17701 cagccgtcat tctgggcacc gtgtggacgt ttaacacctt caatatttca ttctttatta
    17761 accagtatga attggaaagc agtgatattc tggtcacagc tctgttccgc gcggcgttcc
    17821 agtacaaccg ttatggattc tcggcagcct ttgcattcat catcttcttt atccttctga
    17881 tctactcaac catctatgcc agatcaaccg gcgcattgaa aggagtgaag gactaatgac
    17941 cactcagcat gcaaccataa acccctccac actcgagcag aaacagccga aaaaaagaaa
    18001 gtcgaatttc tttcgagcaa accgtggaga cagcaaattc aaacgactga tcatccacat
    18061 tatcctcatc ctgtactgcc tgatttcggt ataccccttg ctgcgcatct ttagcgtttc
    18121 tctgcgccca ggcaaccgtg tgctttctac agatctttct atcatccctc ctgatgcaac
    18181 gtttgaaaac tatgaaaaag tcatcttcga gcaaggtctt tttcaatgga tctggaattc
    18241 actggcagtt accatcacca ccgccattat cggtgtgatc atcgctgcaa cttcggcata
    18301 tgccttttcc cgctataaat tcaaggggag gacaaccctg ctgatctacc tgttgacaac
    18361 gcagatgatc ccgactgcca tgctgatggt gcccttgtac atactagcca tcaatttgaa
    18421 cctcattggc acctatcgcg gtctggtcat cgcctattcc gtcacgtcag tacctttcag
    18481 catctggata ttgaagggct ac
//
LOCUS       DAOSDS010000041        18455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1234_length_18455_cov_4.288804, whole genome shotgun sequence.
ACCESSION   DAOSDS010000041 DAOSDS010000000
VERSION     DAOSDS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18455
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1234_length_18455_cov_4.288804"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1432
                     /locus_tag="PLL88_06290"
     CDS             104..1432
                     /locus_tag="PLL88_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ01213.1"
                     /translation="MTNLLLKNCDVLSFEGGKAHVVAQQDIAIQGNSIAAIQEKDTLA
                     EANYEQCIDASGLLAVPGFINTHAHVPMVLFRNLAEDVTVTEWFNDYIWPMESNLTEE
                     DVYWGTQLGLAEMIENGITCVADHYFFMDEVAKAVELAGTRANLAWAVFAHQGEAMLD
                     KTIDFAKRWQGKANGRITTWLGPHAPYTTTPEFLQLCSQKAKKNDLGIHIHVSETAAQ
                     VDLSLKEYGVTPVQQIRDCGILDVPSILAHVIDPRGEDFDILAKANTGIAQAPKTYLK
                     AGMGLAPIHQYMEYGIPVGLATDGAVSNNTLDILEQMRLFGLFAKYLKSDPTNMKLEE
                     IAEITFRGSAKVMQLPGKIGELKPGMLADIALIRQDGLHATPKANPLAFLTYCAKSTD
                     VDSVLCDGKVLMQGRKLLTLDKARIQQEVQRRLQRLSKRLPAERIAFYPA"
     gene            complement(1433..2020)
                     /gene="plsY"
                     /locus_tag="PLL88_06295"
     CDS             complement(1433..2020)
                     /gene="plsY"
                     /locus_tag="PLL88_06295"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOJ01214.1"
                     /translation="MIAFLPGCLGAYLIGSIPFGLLVARARGVDIRQVGSGNIGSTNI
                     TRALGFRWGLVVAMLDAAKAYFPALLARQFSPHTWQMLVISLMPVIGHIFSVWMGFKG
                     GKGVAATFGLLAFYFGLPLFAAFMVIWYLSVKCVKLMSLVNLVVGLLLPLVFWWKYPT
                     TPYVLFGIVLCAIIWWSHRENIKRLLKGTELPTNY"
     gene            2155..2517
                     /locus_tag="PLL88_06300"
     CDS             2155..2517
                     /locus_tag="PLL88_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOJ01215.1"
                     /translation="MPKKPFTRELPKMNIEADMPSLAEGIVRMNQFINEAQHSGRRVI
                     KIIHGYGSSGVGGRLRGGLRQELTKKRQSGKIKDFIPGERFGVNEDTILYLHQYTQLK
                     IDPDFSKNNPGITVIILY"
     gene            complement(2532..6863)
                     /locus_tag="PLL88_06305"
     CDS             complement(2532..6863)
                     /locus_tag="PLL88_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOJ01216.1"
                     /translation="MKQSHTHSFLDNRIWSAILPAALLNSLVLVIFFSALSTQQVVVD
                     RTLNDYQRTTYDILVRAPENVTEIEKEYGLVEANHLNGTAGGITMQQYEQIKAIPGVE
                     VAAPIAMLGYMNRTSMGIAIQEDLPAGIYRVSASDGIMASEEYVTEASLTPYYALHIP
                     ESSGSWGLFSSRYEGLMDEMLRLHLSITLGGASLITDAFLVSLPSAHDRMLIAAIDPE
                     QEARLVHLDDMLVSGNYLPQDPLLIIDHDLLYVPVLFNIHDYIQQVLSIQLEKTDFEL
                     NVSENYAQQTSAIPDRETVESMPLETVYKADLSVQREWHKNAVQLIVKNNEVVDVYQQ
                     NASITGLLYAPSAVQYREMETVPAALPEDTLVLEAIPQGLTQEEPSLFYNFIPEETQN
                     WLDIVQWQVEPEATYRSLSPRSSYLFNFSPYAEGNGQFEIDTATALAGSSLNQVPLET
                     YLPPSATLKYDENGQELENPVTLTPTLNNEGYLISPPDLLISLSSAQQILENACYESV
                     ADEAAGSGFRLKKMDCGLGDDFISAIRVRVGGVDELDANAQARIEAVANAIVQRTGLH
                     VDVMVGSSPQPVLVHIPGYEGIGELGYVEEYWVKKNVNTLIRSGMNLADGLLFTAMLG
                     AGVLLLFNLNLFLMLGRVSEVALYNALGWRRWDIVKRLLGQMLPIALFSILLAALVSL
                     PVIHILSLEIVVGQFLLIVALELLAFMLSALLPAWQISRASPVEYLQKGEAISQPSHG
                     KKTHMRAIVMENILRKPARSLTILLSILLTSALLTLLFLVQTGLEGQLYGTLLGRWIH
                     SSIRPYHLLMGAGALLVTVINIFLSTRVNVNERKDEIGLLSAFGWRRRDVAGIFCREQ
                     VGISLMGAVIGFGIALGVYGSAYQEPPAHTLAWLKSIALGILLPVGLSALASFLPAYR
                     GASLLPLEALREKAGKVRSHLLRCTVVYMGLGLVGLFAVVFLLQSTLFSAVSQDISIA
                     SETPSTSTVAAAATITPAPTPTPVSEKEVPYYHVDVVVDPDQLRMTGQETITYTNTTG
                     ELLKEIVLRLYANLPFGFEELEKDPERGSLYTNRIQLRDVKMDGQPVEVVLSGHNSIA
                     TLRLEEPLAIGQSASIDLSFTLQGISTLNYVGLGSVWSEDSLFPMLAVYDQNGWRRDI
                     TSTPDIVYSQSARFDYSVTLPPDWQIVATGEQVAQETLGSGQIRYSYQTGLVRDVSIS
                     LSQQFEILQRTVGDTTLYLALANPEPDAEFLMDTAESVFTFYENLFGEYPFASLKMVA
                     WRSVRSSGKEYPALVYFGYNISEGTKMQEVIAHEIAHQWWYSTVGNDIFNEAWLDEGF
                     AEYSALLYLRETQGEEVFQDYLKEFQNSAALADAETDGKFKVGSSVEELSATHYYTNF
                     VYAKGVLFLHTLCTQMGEEAFFDGLRAYYRQYQFGVADRQGFLEVMQRFSPLDLRPLF
                     DTWIGMD"
     gene            complement(6892..7164)
                     /locus_tag="PLL88_06310"
     CDS             complement(6892..7164)
                     /locus_tag="PLL88_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01217.1"
                     /translation="MKAKNKIQKVILLGLVAISLFLIAFSIDTPKPVYAEAKYPCYGY
                     VCLDLMSDPRVIACLAIEAGNAWSCTNYCYSGQWFCAGYAQCQVGC"
     gene            complement(7234..7920)
                     /locus_tag="PLL88_06315"
     CDS             complement(7234..7920)
                     /locus_tag="PLL88_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOJ01218.1"
                     /translation="MKSNKKLMTKPESQKNTLSLIIIILLALQVVLSGIILMRLLNLE
                     NILGETETNAPVPVYVEGVSEGDNPSLGPQDAPITIIIFSDFQCPYCASANQLIKELM
                     QEYPDQIRYVHRDFPLEEIHPDAFNTALAARCAGDQGKYWEMNDALFSNQEDLSIPGI
                     NKLVEMLGLDQVQFDTCFESGKFEEKILNDKQTGEEYGVSGTPTLFVNGYQVNGADRD
                     AILNIIEDIL"
     gene            complement(7994..>8887)
                     /locus_tag="PLL88_06320"
     CDS             complement(7994..>8887)
                     /locus_tag="PLL88_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01219.1"
                     /translation="KGEGQANQQLSSPSPDVALQTDKNPPVLHAEHWETPQPLPAPID
                     MEGDELAPFITLAGDELYFFYNTDLSLSEAKRVLDGISGIYYSRKVDRVWSEPEKVVL
                     SEENALDSCVYTDGQTLWFCSKRDGNYKEEGDLYTASRVDGIWQNWQNAGEQLNIDYD
                     VSEVHLSADQNTMIFSAFRYGGFALTDLWQTRRVGEGWSEPANLGDVINSTEIEDYPF
                     LSEDGLELWFTSPSRLGYPGFAIFRSIWQDGAWGEPQEIISNNVSEVSIDRAGNLYFA
                     HFFFNEEEELLGSKIYVAVKK"
     assembly_gap    8888..8897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9051..9941)
                     /locus_tag="PLL88_06325"
     CDS             complement(9051..9941)
                     /locus_tag="PLL88_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01220.1"
                     /translation="MKQALPSVQNPTSEIDPTPPIMHGTGWEVSEALPAPIDGEGNEL
                     EPFITLAGDELYFFYTPDFSVSEAKRALDGVSGIYYSKKVNGAWSIPERLELGGDNAL
                     NGCVYTDGQTLWFCSIREGNYNPKGDYYTAYFLNGSWSNWQNAGELLNRILDIEEIHL
                     STDQSTMIFSAFLPGSYGYADLWQIHREGESWSQPENLGNIINSTEEETFPYLSEDGT
                     QVWFTSSSRLGYPGYAIFRSIWQNGAWGEPQEIISNYVSEVSIDLQGNLYFTHFLYDE
                     NGELLGSKIYVAYKETNSVP"
     gene            complement(10026..10703)
                     /locus_tag="PLL88_06330"
     CDS             complement(10026..10703)
                     /locus_tag="PLL88_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01221.1"
                     /translation="MQVIVENVSKKYQVGDEEITVLDAVSFQIEAGDFLVITGPSGAG
                     KTTLLNLLGTLDAPSAGKILYDGVEAGALHPNQIADFRRDHIGFVFQFYNLIPQLSAK
                     ENVMLPLLPSMKQLDFDLQDRAVALLAEVGLDGKGEKYPYQLSGGEQQRVAIARALVN
                     TPSLVLADEPTGNLDMEIGEQILHILQEVNRKQGVTVVLVTHNAGFTHFGTRVIRIQD
                     QKLHEVR"
     gene            complement(10721..13447)
                     /locus_tag="PLL88_06335"
     CDS             complement(10721..13447)
                     /locus_tag="PLL88_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01222.1"
                     /translation="MYGKKSLVLRRLRTYNKLFLCGLLLVSMGVSLFFILNEINRQQA
                     LEAVQDYWRTDYDILVHPAGSTFLYDAAGNRLVEPNFLSGQQGGITDEQLELINSIDG
                     IEVTAPVAFLGYFPLGMLIEGEKVNDDPANAEAPWLVYKDVRTFTMSDGWQEAAASDT
                     LYTVENHSDDFSLDPYTGALSFTGENGERYLMPSSITSIFANPSSGEKKVWLSGKETW
                     ESALAYYERENMQPIYKYQYNGLFNLYLPVAAIDPQAEQALVGLEDALLEGRYLSSTD
                     TYRGPNDTYSYLIPVLINESSFQNISINIKTYRLTDPAQQNLSQSLSSGGITYLEGMQ
                     SELLGEKITTLHDYFLQYIKNSMARGGVVGATMKTYLRPSPVQYTQIEGQKAALSISP
                     SGTSGYGPIPGVSADPAQGAYRKALVDDFVLVEKHRGYTFEFAPVGIYDLTGLGGSIL
                     NQVPQELYSAPTALLREDGDGNVLQQEVTIIPTNNPLGYLSQPPVVLTTLPAAKFLAQ
                     RDDYISAVRVRVAGVETAGEASQHKIEKVAHEIEKLTGLQVDITLGSSPQTVLVDVQG
                     SEKVEALGKVEELWVRQLVGITLQRDFTRFDALLFAAMFFSFAVFIYTSAALNMTGRQ
                     GEIGVLKTIGWKDRRILGYLLSEALLLALIIGCITYLGTLGVTAWLGQPIALDRAVWI
                     FPLMGGLFALGTGLPFAQAARRSPLSLLGLGELHEEKGKVSDFDMRSLSSKNISRQRS
                     RFTTATLGLIPAFLALILFFFITLIMAGELSGSLLGQHIQILIQPYHYLVMALILLVC
                     QLILLNITTLNISKRQNEIGVLLTAGWKPATILFTFLKETLYSALISALLAALLALGL
                     LSLLQGVFQLQFLWAIPLGLLLAGVMSLIAMLYPRHLVRKTQSADLLQKRSA"
     gene            13659..14732
                     /gene="recA"
                     /locus_tag="PLL88_06340"
     CDS             13659..14732
                     /gene="recA"
                     /locus_tag="PLL88_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOJ01223.1"
                     /translation="MAAKNNEPQVDQAKQAVLDKALDDIKKRYGDGSILRLGEATHME
                     VASIPTGSLSLDIALGVGGIPRGRVSEIFGPEASGKTTICLHLAAEAQKMGGTVAFVD
                     MEHALDPAYAAKVGVNVNDLLISQPDFGEQALEIVQTLVRSGALDLVIVDSVAALVPR
                     SEIEGDMGDATMGMQARLMSQALRKLSGAINQTRTAVVFTNQLRQKIGVMFGNPETTT
                     GGMALKFYSSVRLDVRRIQSIKRQAEIVGNRVRVRVVKNKVAPPFRTAEFDIMYNEGI
                     SKAGDILDLGSELDVITKRGAFYSFGDTRLGQGRENAKDFLQQNPEMCAEVEKAVREK
                     AMGAQLPSFADEEFQDQEDAEAL"
     gene            14810..15673
                     /locus_tag="PLL88_06345"
     CDS             14810..15673
                     /locus_tag="PLL88_06345"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HOJ01224.1"
                     /translation="MKTRERKTQNLTIPVVFAVIVFCILAGIAAARWLDFNLASLLHP
                     GKKQTILTLAEYQSRQATPTPFLPVAEIDLTPTDPAPTEATAAIPTGTTSPSPAEEEI
                     PASYYINGVYGMAQMTTLDCEMRSAVDWARYFGTTIDETEFISKLPRSDDPEVGFVGD
                     IDGAMGQLPPEGYGVYPPPIASILRQYGLNAQAVKNMTYEELQREISHDRPVIVWIVN
                     LPFEIDQSTYTASTGNTVPIARFEHTWIVSGYNLSTVTVVDSAWTYNVVLETFLERWA
                     VLENRAIILAE"
     gene            complement(15737..16996)
                     /gene="ahcY"
                     /locus_tag="PLL88_06350"
     CDS             complement(15737..16996)
                     /gene="ahcY"
                     /locus_tag="PLL88_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOJ01225.1"
                     /translation="MSEYDVKDLKLAEGGSLRMEWAEREMPVLRLIRERFEKEKPLQG
                     VRVSACLHVTTETANLMTTLQTGGADVMLTASNPLSTQDDVAAALVSRYEIPVFAIKG
                     ESNQVYYKHIHAALDHKPHYTMDDGADLVSTLHKERRDLLESIQGGTEETTTGVIRLR
                     AMAVDGALEYPIIAVNDAMTKHFFDNRYGTGQSTLDGIIRATNVLLSGKTFVVAGYGW
                     CARGLAMRARGMGANVIVTEVDPLPALEAVMDGYRVMPMQEAAQLGDFFCTLTGDLNV
                     LDKHHFEQMKDGAIISNSGHFNVEINIPALEEMAVSKRLVRPFVEEYVLKDGRYIYLL
                     GEGRLINLAAAEGHPASVMDMSFANQALSLEYMIKHHAEMEKKVYGVPEEIDRGIAKL
                     KLEAMGVDIDVLTPEQEKYLKSWEHGT"
     gene            complement(17013..17981)
                     /locus_tag="PLL88_06355"
     CDS             complement(17013..17981)
                     /locus_tag="PLL88_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ01226.1"
                     /translation="MAIICTGSIAYDYIMSFPGYFKDHIIPDKLDSISLSFLVDSMVR
                     QKGGTAPNIAYNLALLGESPKLMGTVGIDFDDYRKWLEEKGVDTSLVKVISGEYTASF
                     FANTDLGNAQIASFYTGAMAYSKDLSIQDLNRKEIDLVMISPNDPTAMRRYVRECSEL
                     GIPYLFDPGQQVVRVDPQDIVEGVNGAQSVFINEYEFELIQKHTRMTAGEIINMVEYL
                     VVTCGECGSDVYVKDEQYRIPIVLPVKVADPTGVGDAFRGGFLRGRRLGYDWQTAGQM
                     GALAATYCLEERGTQNHAYTLQEFVARYRENFDDQGKLDSLIKSTN"
     gene            complement(17992..>18455)
                     /locus_tag="PLL88_06360"
     CDS             complement(17992..>18455)
                     /locus_tag="PLL88_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOJ01227.1"
                     /translation="RLTAWELKQMGIPFEIISDNMAGYFLSTGQAQKVFFGADRVAAN
                     GDVANKIGSYMLALAAADNHVPVYSVFPTSTVDLNLGDGKQIPIEERDQDEVLDISLK
                     GQRVVPEGATARNPSFDVTPARLITAYVTDLGVVEQPFSTRYPELLKMKKD"
BASE COUNT         4805 a         5071 c         4347 g         4222 t
ORIGIN      
        1 cgtgccatac ttcctgaaat tgttgcatcg ttcttttctc aggtatagca taccgcgaag
       61 aaacgtgcat gaaaacaacg gttgcgaaaa gaggtaaata tccatgacca atttactgtt
      121 gaagaattgc gatgttctca gctttgaggg tggaaaagcc catgtagttg cccaacaaga
      181 tatcgccatc cagggaaatt ccatcgccgc catccaggaa aaagacaccc tggcggaggc
      241 aaattacgaa cagtgcatag acgccagcgg gttgctggct gtgccaggtt tcatcaacac
      301 gcacgcgcat gtgcccatgg tgctgttccg caacctggct gaagatgtca cggtgaccga
      361 atggttcaac gattacatct ggccgatgga atctaacctg acggaagaag atgtgtattg
      421 gggaacgcag cttgggctgg cggaaatgat cgaaaatggc atcacctgtg tggctgacca
      481 ttatttcttt atggatgaag tagccaaagc agtggaactg gcgggcacac gcgccaatct
      541 ggcgtgggca gtctttgccc accagggcga ggcgatgctc gacaagacca ttgatttcgc
      601 caaacgctgg caggggaaag ccaacgggcg catcaccacc tggcttggac cgcacgcccc
      661 gtacacaacg accccagaat tcttgcaatt atgctcgcaa aaagcaaaaa agaacgacct
      721 gggaattcat atccacgttt ctgaaacagc agcacaggtg gatctgagct tgaaggaata
      781 cggcgtcacc cccgttcaac agatccgcga ttgcggtatt ctggacgtgc cttccattct
      841 ggcacacgtc atcgatccac gcggtgaaga tttcgacatc ctggcgaaag ccaataccgg
      901 catcgcgcag gcgcccaaga cctacctgaa agcaggcatg gggctggctc ccatccacca
      961 atacatggaa tacggcattc cagtgggtct ggcaaccgat ggggcggtaa gcaacaatac
     1021 gctggatatc ctggaacaga tgcgactatt cggcttattc gccaaatacc tgaaatctga
     1081 cccgaccaac atgaagctgg aggagatcgc agaaatcacc ttccgcggca gcgcaaaagt
     1141 aatgcaattg cccggaaaaa tcggcgaatt gaaacccggc atgctggcag atatcgccct
     1201 catccggcaa gatggtttgc acgccacgcc caaagccaac cccctggcat ttttgaccta
     1261 ctgcgcaaaa tcaacggatg tggatagcgt gttatgcgat ggaaaagtgt tgatgcaggg
     1321 gcgcaaattg cttacccttg ataaagcgcg catccaacaa gaagtacaga gacgcctgca
     1381 gcgtttgagc aaacggctgc ccgctgagcg cattgccttt tatccggcat gatcagtaat
     1441 tcgtggggag ttctgtccct ttcaacagcc gcttgatatt ctcgcggtga ctccaccaga
     1501 taatggcgca caaaacgatc ccgaacagca cgtacggggt cgttggatac ttccaccaaa
     1561 acaccaacgg cagcagcaac cccaccacaa gattgaccag gctcatcaat ttcacacatt
     1621 tcacgctcag ataccagatc accatgaagg ctgcaaagag cgggagacca aagtaaaaag
     1681 ccagcaagcc aaaggtggca gccaccccct tgccgccttt gaagcccatc cataccgaaa
     1741 aaatatgccc gatgaccggc atcagcgaaa tgaccagcat ctgccaggta tgtggagaga
     1801 attgccgggc aagcaaagcg ggaaaatagg ctttggcagc atccagcatc gccaccacca
     1861 atccccaacg aaatccgagc gcccgggtga tattggtgct gccgatgttt ccgctgccca
     1921 cctgacggat atcaacgccg cgcgccctgg caaccagcaa cccaaaaggg atcgagccaa
     1981 tcaaataggc tcccaaacag ccaggcagaa aagcgatcat atccatctct tttaataact
     2041 accctgatcg aaaaaagttg cgcccgtctg ttttcgcctt gttttcgcca aattctgcaa
     2101 tgtttgataa gaaaaccctt ttttgttatc atttttcaac ggaaggagac acgcatgcca
     2161 aagaaaccat ttacccggga actccccaaa atgaacatcg aagccgacat gccctctttg
     2221 gctgaaggca ttgtgcgcat gaaccagttc atcaatgaag cgcaacattc cggcaggcgg
     2281 gtgatcaaga tcatccatgg gtatggatcg agcggcgtgg gcggaagatt gcgcggcgga
     2341 ttgcgccagg aattgaccaa aaagagacaa agcggcaaga tcaaagattt cattcccgga
     2401 gaacgctttg gggtcaacga agacacgatc ctctacctgc accaatatac gcagctcaaa
     2461 attgaccccg atttcagcaa gaacaacccg ggcatcaccg tgatcatcct gtattagtct
     2521 tttggatcag ctcaatccat tccgatccat gtatcaaaga gggggcgaag gtcaagcgga
     2581 ctgaagcgct gcatcacttc caggaatccc tgccgatccg ccaccccaaa ctgatactgc
     2641 cggtaatagg cacgcaaccc gtcgaagaaa gcttcttccc ccatttgggt acacaaagta
     2701 tgcagaaaaa gcacaccctt tgcgtaaacg aagttggtgt agtagtgggt tgccgataac
     2761 tcctccaccg agctgccaac cttgaacttt ccatctgttt cagcatccgc cagagcagcg
     2821 ctattttgaa attccttcaa gtaatcctga aacacctctt cgccctgggt ttcccgcaga
     2881 tacagcaagg cagagtattc tgcaaaacct tcatccaacc aggcttcgtt gaaaatatca
     2941 ttccctaccg tgctgtacca ccactgatgc gcgatttcgt gcgcaatcac ttcctgcatt
     3001 ttggttcctt cgctgatgtt gtacccaaaa tagaccagag cggggtattc cttgccgctg
     3061 gaacgaacgc ttcgccaggc aaccattttc aaggaagcga agggatattc gccaaaaagg
     3121 ttttcataga aagtaaagac actttccgcg gtatccatga gaaactcggc atccggttcc
     3181 ggattggcaa gagccaggta cagggtggta tcgccaacgg tgcgctgtag aatctcaaac
     3241 tgctggctga gtgagatgct cacatcgcgc accaaaccag tctggtagga atagcgtatc
     3301 tgtccggagc ccaacgtttc ctgtgcaacc tgctctcccg tcgctacgat ctgccaatcg
     3361 gggggaaggg tgaccgaata atcgaagcgg gcgctctggc tgtagacaat atccggcgtg
     3421 gaagtgatat cccggcgcca gccgttttga tcgtagaccg ccagcatggg aaagagcgag
     3481 tcttccgacc atacggagcc caagcccaca taattcagag tagaaatgcc ctgcaaagta
     3541 aatgacagat caatggaggc agactgcccg attgccaacg gctcttccaa tcgcagggtg
     3601 gcgatactgt tatgtccgct cagcaccact tccaccggct ggccatccat tttcacatcc
     3661 cttaattgaa tacggttggt gtacaaactg ccgcgttcag gatccttttc cagttcttcg
     3721 aacccaaaag gcaggttggc atacagccgc agcactattt ccttcagcag ctcaccggta
     3781 gtgtttgtat aggtaatggt ctcctgccct gtcatgcgca actggtccgg atcaaccacc
     3841 acatccacgt gataatacgg aacctccttc tccgaaacag gggttggcgt gggagcaggc
     3901 gtgatggtgg ctgctgcagc gaccgtactg gtggaaggcg tctccgaagc aatggaaata
     3961 tcttgagaaa cagcggagaa aagggtactt tgaagcagaa agaccactgc gaacaatccc
     4021 accaaaccca gccccatata taccacggtg cagcgcagca ggtggctgcg cactttaccc
     4081 gctttttcgc gcagcgcttc caacggcagc aagctggcgc cgcggtaagc cggcaggaat
     4141 gaagcaaggg ctgaaagccc caccggcagc agaattccca gcgcgatgct tttcagccag
     4201 gcaagggtgt gcgccggagg ttcctggtag gcgcttccat aaacgcccag cgcaatacca
     4261 aaaccgatca cagcgcccat caggctgata ccgacctgct cgcggcagaa gatgcccgcc
     4321 acatcccggc ggcgccagcc aaacgcgctg agcagcccga tctcatcttt gcgttcattg
     4381 acattgacgc gcgtggaaag aaagatattg atcacggtca ccaacaacgc acccgccccc
     4441 atcaacaggt gatagggccg gatggaactg tggatccaac gacccagcag cgtaccgtac
     4501 aactgcccct ccaatccggt ttgcaccaag aacagcaggg tcaacaacgc actggtcagg
     4561 agaatgctga gcaagatggt cagggaacgc gctggcttgc gcaggatgtt ttccatgaca
     4621 atggcacgca tgtgcgtttt tttaccatgg gaaggttgcg aaatggcttc ccctttctgc
     4681 agatattcaa ccggagaagc tctggaaatc tgccaggcgg gcagtaaggc actgagcatg
     4741 aacgccagca attccagtgc cacgatcaac aggaactgcc ccaccacgat ttccaatgac
     4801 agaatatgaa tgaccggcag ggaaaccagt gctgccagca ggatgctgaa caaagcaatc
     4861 ggcagcatct gccccagtag ccgtttgacg atatcccagc gccgccatcc cagcgcatta
     4921 tacagcgcca cctcggaaac cctgcccagc atcaaaaaca aattgaggtt gaaaagcagc
     4981 agcactcctg cgcccagcat agcagtgaac agcaagccat ccgccagatt catgccgctg
     5041 cgaatgaggg tattgacatt cttcttaacc cagtattcct caacatagcc caattcacca
     5101 atcccctcat aaccggggat atgcaccagc accggctgcg gagaagaacc gaccatcaca
     5161 tccacatgca gcccggtgcg ctgtacgatc gcattcgcca ccgcctcaat gcgcgcctgg
     5221 gcatttgcat ccagctcatc gacgccgccc acccgcacgc ggatggcgga gatgaagtca
     5281 tcccccaagc cacaatccat ctttttcaag cgaaagcccg agccagccgc ctcgtcagcc
     5341 accgattcat agcaagcatt ttccagaatc tgttgggcag agctgagaga aatgagcaga
     5401 tcggggggag aaatcaggta cccctcattg ttgagggtgg gggtaagggt aacaggattt
     5461 tccagctcct gcccattttc atcatatttc aaagtcgcgg aggggggtaa ataggtctcc
     5521 aacggtacct gattgagcga ggaaccagcc agagcagtag ccgtatcgat ctcgaattgc
     5581 ccgttgcctt ctgcataggg actgaagtta aataaataag aagagcgggg tgaaagcgag
     5641 cggtatgtcg cttcaggttc cacttgccat tggacgatat caagccaatt ctgtgtttct
     5701 tcgggtatga aattatagaa taaactcggc tcctcctgcg taagtccctg cggaatggct
     5761 tccagcacca gcgtatcctc tggcagcgca gccggcacag tctccatttc gcggtactgc
     5821 accgcgctgg gggcatacag caaccctgtt atcgaagcat tttgctgata aacatccacc
     5881 acttcattat ttttgacaat caattggacg gcattcttat gccactcgcg ctgcacggaa
     5941 aggtctgcct tatataccgt ctccagcggc atgctttcca ctgtctcccg gtcggggatt
     6001 gcgctggtct gctgagcata attttcgctc acgttcagct cgaaatccgt cttctccaac
     6061 tggatagaaa gaacctgctg aatatagtca tggatgttga acagcaccgg aacgtagagc
     6121 aaatcatggt caattattaa cagtggatct tgcggcaaat aattgccgga taccagcatg
     6181 tcatccagat gcacgagcct ggcttcctgc tcaggatcga tggcagcgat cagcatgcga
     6241 tcatgggcac tggggaggga caccaaaaac gcatccgtta tcaggctagc gccacccagc
     6301 gtgatcgata aatgcaaccg cagcatttca tccatcaatc cttcatacct ggatgaaaaa
     6361 agcccccaac tgccggacga ttcagggatg tgtagggcat aataaggcgt aaggctggct
     6421 tctgtcacat actcctcact cgccatgatg ccatcgctgg cgctcacccg gtagatgccg
     6481 gcgggcaggt cttcttgaat ggcgatcccc atagaggtgc gattcatata acccagcatg
     6541 gcgatggggg cagccacctc caccccaggg atggctttga tctgttcgta ctgctgcatg
     6601 gtgatgccgc cggctgtgcc gttcagatgg ttggcttcca ccaagccata ttctttttcg
     6661 atctcggtca cattttcagg cgctcgcacc aaaatatcgt aggtggtgcg ctgataatcg
     6721 ttaagagtgc gatcgaccac cacttgctgg gtggaaagcg ctgagaagaa gatcaccagc
     6781 accagactgt tcagcagcgc cgccgggaga atggcgctcc agatacggtt atccaagaag
     6841 gagtgggtat gggattgttt catatgaagt ctatggtttc acaatatctt tttaacaccc
     6901 aacttggcat tgtgcatagc ccgcgcaaaa ccattgccca ctataacaat agtttgtaca
     6961 ggaccatgca tttcctgctt caatagctaa acaagctata acccgtgggt cactcattaa
     7021 atcaagacaa acatatccat aacacggata ctttgcttca gcatatacag gtttgggtgt
     7081 atcaattgaa aacgcaatca ggaacagtga tatcgccact aatcctaata aaataacctt
     7141 ctgtatttta ttttttgctt tcatttcatc ctcccaaatt atttatgttt ttgcctcata
     7201 tcttttccct atgcccaccc ttcctgggaa actttataaa atatcttcaa taatattaag
     7261 aatcgcgtct ctatctgcgc cattcacctg atacccattg acaaataagg taggagtgcc
     7321 agacacccca tattcttctc cagtttgctt atcattaaga atcttttcct caaattttcc
     7381 tgattcaaag caagtgtcga attgaacttg atccagaccc aacatttcaa ccaatttatt
     7441 gatacccgga atactcaaat cttcctgatt ggaaaaaagg gcatcgttca tctcccaata
     7501 tttaccctga tcaccagcac agcgagcagc aagagcagta ttgaaagcat cagggtgaat
     7561 ctcttcaagc ggaaaatcgc gatgaacata acgaatctga tcaggatact cctgcatgag
     7621 ctcttttatc aattgattcg ctgacgcaca ataaggacac tgaaaatcac taaaaatgat
     7681 gatcgttatg ggagcatctt gtggccccaa tgaagggtta tccccttcac tgaccccttc
     7741 aacatacact ggaaccggtg cattcgtttc tgtttctcct aatatatttt ccaggtttag
     7801 cagccgcata aggataattc ctgataagac aacctgcaat gctaaaagga tgataataat
     7861 caagcttagg gtattctttt gtgattctgg tttcgtcatc agttttttgt tgcttttcat
     7921 tattcctctc tagttttcta aaccttattg acacatcttc ttgtgaaata gcattgaaga
     7981 ttcctcactg aatctatttt tttacagcca cataaatctt tgaccccagc aactcttctt
     8041 cttcattaaa gaaaaaatga gcaaaataca gattccccgc cctatcaatg gaaacctcgc
     8101 taacattgtt ggaaatgatc tcctgcggtt ctccccacgc accatcctgc cagatcgagc
     8161 ggaaaatggc aaatcctgga tagcccaaac ggctgggaga agtaaaccat aattccaacc
     8221 catcctccga gaggaaagga tagtcttcta tttcagtgct gttaataaca tcccccaaat
     8281 tcgccggttc agaccagccc tccccaacac gccgggtttg ccatagatct gtcagagcga
     8341 atcctccata acgaaaagcg gagaagatca tggtgttttg atccgcggag agatgtactt
     8401 cgctcacatc gtaatctata ttcaattgct ctcctgcatt ctgccaattc tgccagatgc
     8461 catccacccg ggaagcggta taaagatcac cttcttcctt ataatttcca tcacgcttcg
     8521 aacaaaacca gagcgtttgc ccatcggtat agacacagct atccaaagca ttttcttctg
     8581 acagcaccac cttttcaggt tctgaccaca ctcgatctac ctttcttgaa taataaatac
     8641 ctgaaatacc atctaatact ctcttggctt cggaaaggga aagatcggta ttatagaaaa
     8701 aatacaactc atcgcctgcc agcgtgatga aaggcgccaa ttcatctccc tccatatcaa
     8761 taggagccgg cagcggctgc ggagtttccc aatgctctgc atgcaaaaca ggaggattct
     8821 tatccgtttg aagagcgacg tccggtgaag gagatgaaag ttgctggtta gcttgcccct
     8881 ctcctttnnn nnnnnnntta tcgttactac aagcggcact gaaaagaaga agcactaggc
     8941 aaaggtatct gagcaatctc gaaaatttcc attgcatgct tcatctcact ttctgtattt
     9001 catttctgtt acgaagaaat atatacagga aacagtttcg tcttaccaga ttaaggaact
     9061 gaattggtct ctttatacgc cacatagatt ttagatccca acaactcacc attctcatca
     9121 tagagaaaat gggtaaaata cagattcccc tgcagatcaa tcgatacctc actaacgtaa
     9181 ttggaaatga tctcctgcgg ttctccccac gcgccattct gccagatgga acggaaaata
     9241 gcataaccag ggtaacccaa gcgactggaa gaagtgaacc atacctgtgt accatcctcc
     9301 gaaagatagg gaaaagtctc ttcctctgta ctgttgataa tattccccag attttcaggt
     9361 tgtgaccagc tttctccttc gcggtggatc tgccagagat ccgcataacc ataactaccc
     9421 gggagaaacg cagagaagat catggtactc tgatctgttg agagatggat ttcttctata
     9481 tcaagaatac gattcaataa ctcccctgca ttttgccaat tgctccaact tccattcaga
     9541 aaataagcag tatagtagtc accctttgga ttgtaatttc cttcgcgtat ggaacagaac
     9601 caaagcgttt gcccatcagt gtaaacgcaa ccattcaagg cattatcccc acccagctcc
     9661 agcctttcgg ggatagacca cgcgccattc actttttttg aataataaat acccgaaaca
     9721 ccatccaaag cccgttttgc ttccgaaaca gaaaagtccg gtgtatagaa aaaatacaac
     9781 tcatctcctg ccagcgtgat aaagggttct aattcattcc cttctccatc gatgggagcc
     9841 ggcagcgctt cagaaacttc ccaaccagtc ccatgcatga tgggaggagt ggggtcaatt
     9901 tctgaggtgg ggttctgtac agatgggagc gcctgtttca cggcaggttc ctccttacca
     9961 acgcaagcag aactgacaat gatcaccatg cagcaaaacc caacacatat accgtgtcgc
    10021 atagctcatc gaacctcgtg caatttctga tcttgtatcc tgatcacccg tgttccaaag
    10081 tgggtgaaac ctgcgttatg cgtcaccagc accaccgtca cgccttgctt tcggttgacc
    10141 tcctgcaaaa tgtgcaggat ctgttcaccg atctccatat ccagattgcc ggtgggctca
    10201 tccgccagca ccaggctggg agtgttgacc agcgcgcgcg caatagccac gcgctgctgt
    10261 tcgccgcccg aaagctggta gggatatttc tcgcccttgc catccagccc cacctccgcc
    10321 aatagagcta ccgccctatc ttgcaggtcg aaatctaact gtttcatgga aggcagcaaa
    10381 ggcagcatga cgttttcttt ggcggaaagc tgcgggatga ggttatagaa ctggaagaca
    10441 aagccaatat gatcacggcg gaaatccgcg atctggttgg ggtgcagcgc gcccgcttct
    10501 accccgtcat acaaaatctt gcccgcgctg ggtgcatcca gcgtgcccag cagatttagc
    10561 aaggtggtct ttccggcgcc ggaaggtccg gtgatcacca gaaaatcccc cgcctcgatc
    10621 tgaaagctca ccgcatccaa aacggtgatc tcttcatctc ccacttgata tttcttgctc
    10681 acgttttcaa caatcacctg catagatcaa tcccttctct ttatgccgat cttttctgca
    10741 acaaatcagc gctctgtgtt ttacggacca aatggcgcgg gtaaagcatg gcgatcaaac
    10801 tcatcacccc cgccaacagc agtcccagcg ggatagccca taaaaattga agctgaaaaa
    10861 ccccttgcag caacgaaagc aatccaagcg ccagcaaggc agccagcaaa gcgctgatca
    10921 gcgcgctgta cagggtttct ttcaagaaag tgaataaaat tgtagctggt ttccagccag
    10981 cggtgagcag cactccgatc tcgttctggc gcttgctgat attgagggta gtaatattca
    11041 acaggatcaa ctggcacacc agcaggatga gcgccatgac cagataatga taaggctgga
    11101 tgagaatttg aatatgctgc ccgagcaaac tgccgctcaa ttcgcccgcc atgatgaggg
    11161 tgatgaagaa gaataggatg agcgccagga aagctggaat gagccccagt gtggcggtgg
    11221 tgaagcgcga acgctgcctg gaaatattct ttgaagataa cgagcgcata tcgaaatcag
    11281 atacttttcc tttttcctcg tgcagttccc ccaagccaag caaagacagc gggctgcgcc
    11341 gggctgcctg cgcaaagggg agtcccgtgc ccaaggcaaa caagcccccc atgagcggga
    11401 agatccatac tgcccgatcc aaggcaatgg gctgccccag ccatgcagta acacccaacg
    11461 tgcccaggta tgtgatacaa ccgatgatta aagccagcag cagcgcctcc gataacaaat
    11521 accccagaat acgccggtcc ttccagccga tggttttcaa caccccgatc tccccctgcc
    11581 tgccggtcat attgagcgca gcgctggtat agatgaaaac tgcgaaagag aaaaacatgg
    11641 ctgcaaacag cagcgcgtca aaacgggtga aatcgcgctg cagggtgatg cccaccaact
    11701 ggcgcaccca cagttcttcc accttgccca gcgcttccac tttttccgaa ccctgcacgt
    11761 ccaccaacac cgtctgcgga gaagagccga gggtgatatc cacctgcaag ccggtgagtt
    11821 tctcgatctc gtgcgccact ttttcaatct tatgttgcga ggcttctcca gccgtctcca
    11881 cccccgccac cctgacgcgc accgcgctga tgtaatcgtc acgctgtgcc aagaatttag
    11941 ccgccggcag agtggtcagc accaccggcg gctgcgacag ataacccagc ggattgttgg
    12001 tcggaatgat ggtcacctct tgctgcagca catttccatc gccgtcctcg cgcagcagcg
    12061 cggttggggc agaatagagt tcctgcggaa cctgattgag gatggagcct ccaagaccgg
    12121 taagatcata aatacccacc ggtgcaaatt caaacgtata tcctctatgc ttttcaacca
    12181 gaacaaaatc atctaccagc gcttttcgat acgctccctg agcaggatca gcggacacgc
    12241 cgggaatagg accatacccg ctggttccag acggagaaat gctcaaagca gccttttgcc
    12301 cctctatttg cgtatattgg acagggctgg gacgcagata agtcttcatc gttgcaccaa
    12361 caacccctcc gcgtgccatt gaattcttta tatactgtaa aaagtaatca tgcagcgtgg
    12421 tgattttctc ccccagcagc tcgctctgca tgccttccag ataagttatt cctccgctgc
    12481 tcagagattg cgagaggttc tgctgcgcgg gatccgtcag gcgataggtt ttgatattga
    12541 tggagatatt ttgaaaggaa ctctcattga tcagcaccgg gatgagatag gaatacgtat
    12601 cgttaggtcc cctataggtg tcggtggagg atagatatct gccttccagc agcgcatctt
    12661 ccaaacccac cagcgcctgc tccgcctgcg gatcgatggc agccacgggt agatagagat
    12721 tgaacaaccc gttgtattga tatttataga ttggctgcat attctcgcgc tcataatatg
    12781 ccagagcact ttcccaggtc tctttcccgc tgagccagac cttcttctcc ccactcgaag
    12841 gattggcaaa gatggaagtg atgcttgacg gcattaagta tctctccccg ttttcaccgg
    12901 taaagctcaa agcccccgta taagggtcca gagaaaaatc atcgctgtga ttttcaacag
    12961 tatataaggt atccgaagcg gcagcctcct gccagccatc gctcatggtg aaggtgcgca
    13021 cgtccttgta aaccagccag ggcgcttccg cgttggccgg atcatcattc actttttcgc
    13081 cttcgatcaa catgcccagc gggaaatacc ccaggaacgc caccggagcc gttacctcga
    13141 tgccatcaat ggaattgatc aattccaatt gttcatcggt gatgccgccc tgctgaccgg
    13201 ataagaaatt cggctcgacc aggcgattgc cggctgcatc gtacaaaaaa gtactgcccg
    13261 ccgggtgtac taaaatatcg taatcggtgc gccagtaatc ctgcacagct tccagcgcct
    13321 gctggcggtt gatctcattc aagatgaaga acaaacttac ccccatggaa accagcagca
    13381 aaccgcacag gaacagcttg ttgtacgttc gtagccttct caaaacaaga cttttctttc
    13441 catacattcg tacccctttt tcaaaccccg ctacctgtaa gaacacttat aagaacagtc
    13501 aagcccatta tagcactgtc cctattcgca ccgttcgaaa tatgacaaag gtatggtatt
    13561 ttcttgccat taagcaaatt tagaacatcc ttgctattat tggattgatg gagatataat
    13621 atactcatca aagaattcat ttggtggagg agaaagccat ggctgcgaag aacaatgaac
    13681 cccaggtaga tcaagccaaa caagcagtgt tggataaagc gttggacgat atcaaaaaac
    13741 gctacggaga tggctccatc ctgcggctgg gagaagccac ccatatggaa gtggcttcca
    13801 ttccaaccgg ttccttatcc ctggatatcg cgctgggcgt gggcggcatt ccacgcggca
    13861 gggtgagcga aatcttcgga ccggaagcat ccggcaagac caccatttgt ctgcacctgg
    13921 cagccgaagc ccaaaaaatg ggcggcacgg tagcttttgt ggacatggag cacgcgcttg
    13981 acccggcgta tgccgccaag gtgggcgtga acgtgaatga cctgctcatc tcgcaacctg
    14041 atttcggcga acaggcgctg gagatcgtgc aaacgctggt acgctccggc gcgctggacc
    14101 tggtgatcgt ggactcggtg gcagcgctgg tgccccgctc ggagatcgaa ggcgacatgg
    14161 gcgacgccac catgggcatg caggcacggt tgatgtcgca ggcattgcgc aaactttccg
    14221 gtgccatcaa ccaaacccgc acggctgtgg tcttcaccaa tcagctgcgc caaaagatcg
    14281 gcgtcatgtt cggcaacccc gaaaccacca ccggtggaat ggcgctcaaa ttctattcct
    14341 ccgtgcggct ggacgtgcgc cgcatccaat ccatcaagcg ccaggcggag atcgtcggca
    14401 accgcgtgcg cgtgcgcgtg gtcaaaaaca aggtcgcgcc acccttccgc acggcagagt
    14461 tcgacatcat gtacaacgaa ggcatctcca aggcgggaga catcctcgat ttgggctccg
    14521 aactggatgt gatcaccaaa cgcggcgcat tctactcctt cggagacacc cggctcggac
    14581 aggggcgcga aaacgccaag gatttcttgc aacagaatcc cgagatgtgc gccgaggtgg
    14641 agaaagccgt gcgcgaaaaa gccatgggcg cgcaattgcc cagctttgca gacgaagagt
    14701 tccaggacca agaggacgca gaggctcttt agcaaaagac caccatcagg agaaaaacaa
    14761 gatgctgcta taattggcag cattcttgca ttaaagacca tgaacaatca tgaaaactcg
    14821 cgaaagaaaa actcaaaatc ttactattcc ggttgtcttt gcggtgatcg ttttttgcat
    14881 tctggctgga attgcagcgg cgcgctggct ggatttcaat cttgccagtc ttctccatcc
    14941 tgggaagaaa caaaccatcc tcacgctggc tgaataccaa tccaggcagg caacacctac
    15001 cccatttctt cccgttgcag agatcgatct caccccaacc gatccagctc caactgaagc
    15061 aacggcggca atacccaccg gcacaaccag cccctcacct gcggaagagg agatccctgc
    15121 atcctattac atcaacgggg tttatggaat ggcgcagatg accacgctgg attgcgagat
    15181 gcgttcggcg gtcgattggg cacgctattt tggcacgacc atcgacgaaa ccgaattcat
    15241 ttccaagctt ccccgttccg acgatcccga agtggggttc gtgggcgata tcgatggagc
    15301 gatgggacaa ctccctcccg aaggatacgg cgtttacccg ccacccatcg ccagcatcct
    15361 gcgccaatat gggttgaacg ctcaggcagt gaagaacatg acctacgaag agctgcaaag
    15421 ggaaatctcc cacgaccgcc cggtgatcgt ctggatcgtc aacttaccct ttgagatcga
    15481 tcaatccaca tacaccgcct ctacggggaa caccgtaccc attgcccgtt ttgagcatac
    15541 ctggatcgtt agcggctata acttatccac ggtaacggta gtggacagcg cctggactta
    15601 taatgtggtg ctggagacat ttttggaacg gtgggcggtt ttggaaaacc gtgcaatcat
    15661 ccttgcggaa taaaaaagga aacagggaaa atttctatca cctgtttcct ctcttttacg
    15721 acttttctga cgatctctat gtgccgtgtt cccaggattt caaatatttt tcctgttcgg
    15781 gggtaagcac atcaatatcc acgcccatgg cttccagttt cagcttggca atgccccgat
    15841 caatctcttc gggcacgccg taaacttttt tctccatttc ggcatggtgt ttgatcatgt
    15901 attccaaact gagcgcctga ttggcaaaag acatatccat cacgctggct gggtgccctt
    15961 cggcagcagc caggttgatc agacgacctt cgcccagcaa atagatatag cgtccatctt
    16021 tcaacacata ttcctctacg aaggggcgaa ccaggcgctt ggaaacagcc atttcttcca
    16081 gcgcagggat attgatctca acattaaagt gcccagaatt cgaaatgatg gcgccatctt
    16141 tcatttgttc gaagtggtgt ttatcgagca cgttcaggtc accggttaaa gtgcagaaga
    16201 aatcgcccaa ttgcgccgct tcttgcatgg gcatcactct gtaaccgtcc atgaccgctt
    16261 ccagtgctgg caaggggtcg acttccgtga cgatcacatt ggcacccatg cctctggcgc
    16321 gcatagccag accgcgggca caccaaccat acccggcaac cacaaatgtc tttcccgaaa
    16381 ggagcacatt ggtagcgcgg ataataccat ccaacgtaga ttgccctgtg ccgtagcgat
    16441 tatcgaagaa gtgcttggtc atggcatcat tgactgcaat gatgggatac tccaacgccc
    16501 cgtcaaccgc catggcgcgc aaccgaatga caccggtggt ggtctcttcg gtgccaccct
    16561 gaatactttc taacaggtca cggcgttctt tgtgcaatgt gctgaccaga tcagccccgt
    16621 catccatggt gtaatggggt ttatgatcga gcgctgcatg gatatgcttg taatagacct
    16681 gattgctctc acccttgatg gcgaaaaccg gtatttcata gcgcgatacc agggcggctg
    16741 ccacgtcatc ctgggtggaa agcgggttgg acgcggtgag catcacatcg gcgccacctg
    16801 tttgcagagt ggtcatgaga ttagccgttt ccgtggtcac atgcaaacaa gcagaaacgc
    16861 gcaccccctg taagggtttt tccttttcga aacgttcgcg gatcaaacgc aaaaccggca
    16921 tttcgcgttc agcccattcc atacgcagag atccaccctc tgctaatttc agatctttta
    16981 cgtcgtattc agacatggat gtattcctct ctttagttgg ttgattttat cagggaatcc
    17041 agttttccct ggtcatcaaa gttttcacga tagcgtgcca caaattcctg caaagtgtag
    17101 gcatgatttt gtgttccccg ttcttcaaga cagtaggtgg cagccaacgc gcccatctgc
    17161 ccggcggttt gccaatcata acccagccgg cgaccgcgca agaaaccacc gcggaaggca
    17221 tccccgaccc cggtaggatc ggcaacttta accggcagaa caatgggaat gcgatactgt
    17281 tcatctttca catacacatc cgagccacat tcaccgcagg tcaccaccag atattcaacc
    17341 atgttaatga tctcgcctgc ggtcattctg gtatgtttct ggatcaattc aaattcatat
    17401 tcgttgatga acacactctg cgccccgttc accccttcca caatatcctg ggggtccacc
    17461 cgaaccacct gctgacccgg atcgaacaaa taagggatgc ccaattcgct acattctctc
    17521 acgtagcggc gcatggcagt ggggtcattg ggagaaatca tgaccaggtc aatttctttt
    17581 ctgttcagat cctgaatgga taaatctttt gaataagcca tcgccccggt gtagaagctg
    17641 gcgatctgcg catttcccag atcggtattg gcaaagaacg atgccgtata ttcccctgag
    17701 atcaccttta ccagcgaagt atcaacaccc ttctcttcca gccattttct ataatcgtca
    17761 aaatcgatcc ctaccgtgcc catcaatttg ggcgattctc ccaacaatgc caggttataa
    17821 gcaatgttgg gggcggtgcc gcctttttgg cgcaccatcg aatcgaccag gaaagacagg
    17881 ctgatggaat ccagcttatc cggaatgatg tgatctttga aatagccggg aaaagacatg
    17941 atgtaatcat acgcgatgga accagtacat ataattgcca taattccctc tttagtcctt
    18001 cttcattttc aggagctctg gatagcgggt cgaaaaaggt tgttcgacca cacccaggtc
    18061 ggtaacataa gcggtgatca aacgtgccgg cgttacatca aaagacggat ttcgagcagt
    18121 cgccccttcg ggtaccacac gctgcccttt taatgagata tccagcacct catcctgatc
    18181 acgctcttcg atcgggattt gctttccgtc tccaaggttg agatctaccg tagaggtcgg
    18241 aaaaacacta tagaccggga catgattatc cgcggcagcc agcgccagca tataggaacc
    18301 gatcttgttc gccacatccc cattggcagc cacgcggtcc gctccaaaaa agaccttttg
    18361 ggcttgcccg gtgcttaaaa aataacccgc catattatcg ctgataatct caaaaggaat
    18421 gcccatctgt ttcagttccc aggcagtcaa cctgg
//
LOCUS       DAOSDS010000042        18274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1245_length_18274_cov_5.189582, whole genome shotgun sequence.
ACCESSION   DAOSDS010000042 DAOSDS010000000
VERSION     DAOSDS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18274
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1245_length_18274_cov_5.189582"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2725
                     /gene="uvrA"
                     /locus_tag="PLL88_06365"
     CDS             <1..2725
                     /gene="uvrA"
                     /locus_tag="PLL88_06365"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOJ01228.1"
                     /translation="SLSAYARQFLGQMSKPDVDYIEGLSPAVSIDQKATSHNPRSTVG
                     TVTEIYDYLRLLYARIGIPHCPVCGKEVVKQSAQEIVDIVAALPADTKFFVLAPVVRG
                     RKGTYQAVFEEIQKAGFVRARVDGQVVNLEEDIVLDRYKIHDIEAVVDRLVIRKAESE
                     DDRSTFLSRLSDSIETALKVGEGYLVVQVLSDEGSRDMFFSEHLACSEHGVSLPAIEP
                     RSFSFNTPHGACPVCQGLGVQQEIDVNLLIPDRELSLREGALVPSEWNGPREEGGYYW
                     QMLEAAAREYKIDLDKPVGELPEEKLNVILYGTQGKEVRMNFSRENRIYEVDSAYEGV
                     IPNLMRRFKETQSDYIRNKIATYMSDKPCTECNGARLRKESLAITVDGVNILQVTEWP
                     VIRTLEWINRLTSENSPLSNKEKIISDRILKEINDRLGFLVNVGLDYLTLQRSAATLS
                     GGEAQRIRLATQIGSSLMGVLYVLDEPSIGLHPRDNARLLKTLMDLRDIGNTVLIVEH
                     DEETIRSADWIIDLGPAAGENGGELVAEGTVKDILAHPSSLTGAYLSRRKVVPLPKQR
                     RNGNGHALTVVGARQNNLKNIDVSIPLGRLVCITGVSGSGKSTLMVDILYNALARKLN
                     RAQTNPGLYERIDGTEYLDKIINIDQSPIGRTPRSNPGTYTGVFDDIRNLFAELPESK
                     MRGYRASRFSFNVKGGRCEACGGNGQIRIEMQFLPDVFVPCEVCHGNRFNRETLQVKY
                     KGKNIAEVMDMTIDSAIDFFSNIPRIVGRLKTLQEVGLGYVRIGQSATTLSGGEAQRV
                     KLSRELSRRATGKTLYVLDEPSVGLHAADVHRLVDVLQALVNAGNSVLIIEHNLDIIK
                     IADYIIDMGPEGGDRGGEVIAQGTPEEVCASAHSYTGEVLKEYIQDFYAQK"
     gene            complement(2793..3005)
                     /locus_tag="PLL88_06370"
     CDS             complement(2793..3005)
                     /locus_tag="PLL88_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOJ01229.1"
                     /translation="MSEKEQGTVKWFNGSKGYGFIERESGEDVFVHYSAIVGNGFRNL
                     EEGQRVQFVVTQGQKGAQAEEVEILK"
     gene            3281..3607
                     /locus_tag="PLL88_06375"
     CDS             3281..3607
                     /locus_tag="PLL88_06375"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ01230.1"
                     /translation="MTRAKEIENILPLTETSFYILLSLIQPLHGYGIIKKIEEMTAGR
                     ILMGAGTLYGALKNLLENGLIVEVESSSDRRRDYQITLKGKDLVLQELIRYEELIKNA
                     RSVVAG"
     gene            3621..4139
                     /locus_tag="PLL88_06380"
     CDS             3621..4139
                     /locus_tag="PLL88_06380"
                     /inference="COORDINATES: protein motif:HMM:NF022641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HOJ01231.1"
                     /translation="MEKKKITKLFFAWQDENEQTWLEQMAAHGWILEEVGFIRYTFRK
                     EKPRHINYRLDYQELKESELQSYLELFADAGWEYIGRLNNWFYFASSGPDTREIYTDV
                     HSKIQKYKRVLRSLIFACIPISYLLIFFPRFTGQATFSDILLFMVFLMALMVVFNIIR
                     LALRVNKMEKGL"
     gene            4282..5820
                     /locus_tag="PLL88_06385"
     CDS             4282..5820
                     /locus_tag="PLL88_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOJ01232.1"
                     /translation="MTSLKKVFVGIKWKLTITYMLATLLMLFLVEVLIAISGNRYAFT
                     SLNFVYSSAQYLSESTASLAQALREPVDADFLNAWITDNEETFLRVSDKDGRFDRKND
                     PQGNTPPLDDGSIVDLTPKTVAFSTSKSTLTIVDANGIILVTNNPLIFPPGQSISAYL
                     SADENTMLATVASAVIPAPQFSIMEQDATTLLLPIRQGDQYVGAILAQYYAPTFWEQV
                     KAAVSGFLPELPVFFIISSAIGILFGFIIASSFTRRLDHMEETTLQWGLGDFSKKAEV
                     RGSDEISTLAVSLNHMADQFQQLLEKEQMLATMEERNRLARDLHDSVKQQIFAISMNL
                     GAIRSLLKTNLKKATQQVEISSKLAQQALDELTSLINTLRPVQLEDQSLQDALHDYLK
                     VWEKQSGVSVVFRTEGAHQPLAREAEQSLFRIVQEALSNITRHSRASAASISLTFEAD
                     HLTLQISDNGVGFDKNLVKQGLGLHSMEERVKEMQGIIKLNSSTAGTTITIVIPRVRE
                     EQEK"
     gene            5817..6479
                     /locus_tag="PLL88_06390"
     CDS             5817..6479
                     /locus_tag="PLL88_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ01233.1"
                     /translation="MTNDKITIMLVDDHAIVRSGIRAYLEVLPDIQIIAEAESGTQAV
                     KLAEQQAPDVVLMDLKMPEMDGVEATWRVRQVSPRSQIIILTSYHEDSHIFPAIKAGA
                     LSYILKTVGPEELANAIRSAARGEVTLSPKVASRLIGEWRDDRSDGMQDYMQLTEREQ
                     EVLALIAEGLSNAEIANRLVISEKTVKSHVSNILSKLHLADRTQAAVFAWREGIIKKE
                     NT"
     gene            complement(6520..7245)
                     /locus_tag="PLL88_06395"
     CDS             complement(6520..7245)
                     /locus_tag="PLL88_06395"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ01234.1"
                     /translation="MNWKKRLLLSIGRTILNIFARLFLEFNVHYEAPLKRGAKIFAAN
                     HPTTTDPFLMSLVTEEPLTVLVNKRIMQISIIGRLIEQAGTIPVDKENKNSQEIIQRS
                     LQVLEQDLPIAVFPEGCLTPSVNMLSELHTGVTRIALAAHATIVPVGIYVNPTYIRRH
                     FFHRKYGLEESRWLLHGKYYITIGKPLRFEGDVENRELVKEFTNQVGLEIKRLMLESE
                     LRAVQKGIAWNPIIPLAREMPRK"
     gene            7420..8640
                     /locus_tag="PLL88_06400"
     CDS             7420..8640
                     /locus_tag="PLL88_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ01235.1"
                     /translation="MKSKRMLTSRFPYALSLFAVLTFACISALDYFIFQGELTKADPH
                     WKKWVLFSSYFLLAAFDLFDMLFYSKTRLFMHTLFFVLRIIVIGFMNSIDITHFSRIL
                     FGLIPYHAYLIFGLVISLLSMIVVLYYFYSISAFIPTPETLPALASVLFFQLTAIFAE
                     RDKKATFENQEIIEELSRSNIELRTYIEDLSRLSIMEERVQLSNDLHDSIGHYLVAIN
                     IQLEKALAYRSISSETVDEAIINAKFSVEQALREVRRFVDGLHDHEDIYSLKEKAQEL
                     ITNFKDVFTTLDFQYEGNEFAFAKIIRRNLFYILQESLTNIQKHAHAQNVVVTINFKA
                     REVVMLVKDDGVGFKAKKSADQAGHYGLSSIHQRVELLNGSVNIASNKKKGTSIEVRI
                     PLKQENPQRGKDHE"
     gene            8633..9295
                     /locus_tag="PLL88_06405"
     CDS             8633..9295
                     /locus_tag="PLL88_06405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ01236.1"
                     /translation="MNDKIRVLIVDDQQLVRDGIKALLEIKEDFSIIGTAADGQEAMD
                     VLQQTPADVVLMDIRMPGLGGIEATRQIRQLNKEIRIIMLTTFNNDDYIVKAIKAGAN
                     GYLLKDIPKDDLAEAIRLAYRGVSQFSSDTIGMLVERNLLRDTDVSPEPEQELSLLNE
                     KEYEILCMIAEGYSNREIAEELFFTEGTVKNYVSKILNTLHLRDRVQAAIYAVQHHLQ
                     RK"
     gene            complement(9438..10022)
                     /locus_tag="PLL88_06410"
     CDS             complement(9438..10022)
                     /locus_tag="PLL88_06410"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HOJ01237.1"
                     /translation="MATYLQYILHFLFNFLTAFVIVRFIYYPQQRSQKFVLTFLGFNT
                     IIFILFNYMASIELSMGVGFGLFAIFSLLRYRTDPMPSREMTYLFILIALPMFNYIMF
                     SQGAYIEVGLTTLLISAVLVFLEKGWGFHFLASKSITYEKIDLVNANNQKELIADLEE
                     RTGLIIHYLDINKINFLNDTAEIKIYYEDVKNER"
     gene            complement(9982..10842)
                     /locus_tag="PLL88_06415"
     CDS             complement(9982..10842)
                     /locus_tag="PLL88_06415"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HOJ01238.1"
                     /translation="MFTTQNQTYSLLLPSLFNRTMAAIEEIVPTYSTVSLHEIEEYAL
                     QDRVDVKFLVRIVDVPSLLSILPGSYSILEVENKRISAYRTLYFDTPCLTFYFDHHNG
                     KRPRYKIRMREYHSTNDTFLEVKKRENELRTVKNRMPIARIRPSFSAEMCAFLAQHYP
                     EDRRNLKAVLRNNFKRITLVNLNACERATIDTGLSFSNRTNSLQLSHLAVVEVKQDNY
                     SRESVILQALREKGYQPMSFSKYCTGVAFLNPDIKRNRFKQNFLQFKASTKRIYKNGN
                     ISPVYTSLSF"
     gene            10906..11028
                     /locus_tag="PLL88_06420"
     CDS             10906..11028
                     /locus_tag="PLL88_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01239.1"
                     /translation="MEFDSACGGICCIGSMVKCFVRSALAAIAVLSMSGGGRLA"
     gene            11103..11342
                     /locus_tag="PLL88_06425"
     CDS             11103..11342
                     /locus_tag="PLL88_06425"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HOJ01240.1"
                     /translation="MTSLDIVAPGSTCVVQAIEGEGAVRTRMMDMGLLKGTVVKVIRV
                     APLGDPIEFEVKGYNLSLRKKEARMVAVQVMDDDA"
     gene            11329..11652
                     /locus_tag="PLL88_06430"
     CDS             11329..11652
                     /locus_tag="PLL88_06430"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOJ01241.1"
                     /translation="MMTLSIAPEGQLLTLVSIKGGLGVQKRLNALGLTPGTEVMIVSQ
                     NEGGPIILAVKDSRLAIGRGLLHHIQVEAKESSLHQTEPLDPQISSYRHVHDRRHRRF
                     GGRHG"
     gene            11645..13888
                     /gene="feoB"
                     /locus_tag="PLL88_06435"
     CDS             11645..13888
                     /gene="feoB"
                     /locus_tag="PLL88_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOJ01242.1"
                     /translation="MDDRENKEEIMEKELIIAVAGNPNCGKSTVFNQLTGTRQHVGNW
                     PGKTVEKKEGSFQYKGQHITVVDLPGTYSLTAYSLEEVIARDYVIKEKPDLVVSIIDA
                     ANIERNLYLTMQLIELGVPVIAALNMMDVVAEREIQIDAEKIEKALRVPVVRMVARDG
                     KGLDELKEKILLIAKTKQEYKKEVPARFNKELEEAIQKLQVKIESMPKDKRSFDSRWL
                     AIKLLEGDKGVLNDLKEEKNGYKSLIEEARLFSQTYEERSDFGIDTEIADTYYSTIHT
                     IVNASVKKPKIQKASFSDKLDRIVTHRVWGIPIFLGMMWIVFKLTSDVAAPFLDWVDG
                     VIGGPLTRWVVSLLTLLRLDGTWVESMLVDGVIAGVGGVLVFVPVMMMLYLALAILED
                     SGYMARAAFVMDRLMHGLGLHGKSFLPMIVGFGCTVPAIYATRTLENEKDRILTSLLV
                     PFMSCGARLPVYVLFATIFFPQNTGLVIFSLYVIGIVMAILVGLLLKNTVFKERDVSP
                     FVMELPPYRRPTLKSIWAQMAERTNAFVKKARGLILVTSICIWALLALPVKGEGRFAD
                     APVSDSAFAAVSSAVAPIFTPAGFGTWEASGSLLTGFVAKEVVVSTMSQIYEAQEAEE
                     EAVATTFLEDIWEIVSSFFVAAWDTIKSIPLIVGINLFNEGEEAEPTVLMAAVHDGFE
                     ASSGGRGALASFAFMVFVLLYTPCMVAIAAERHEIGTKWMWVSVIGQFALAWLVSTLV
                     FQIGSLL"
     gene            13954..14220
                     /locus_tag="PLL88_06440"
     CDS             13954..14220
                     /locus_tag="PLL88_06440"
                     /inference="COORDINATES: protein motif:HMM:NF020581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HOJ01243.1"
                     /translation="MIQRILEEINKNRGFVSLNELAERLELDPNVLSGIMQTLNRNRD
                     LKVETGMPDCSHASCPACSVKNMPSEMICGCQVKPKRSQSDRLS"
     gene            14327..15529
                     /locus_tag="PLL88_06445"
     CDS             14327..15529
                     /locus_tag="PLL88_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HOJ01244.1"
                     /translation="MWHWFNRNHRWITALVGVVVVVVLILLWQTSANNQNVVGYSSTT
                     VKARVTGILEEGTVTMGEKTQTYQVLSVKLLESDQEGQVYELQYGKYQLLSDDYRLQV
                     GDKILVNLESMQDGTYAVYFIDHVRTDSLLVLLCIFIVTGIAMSGWKGLNSMISMAFS
                     ILVMAVYVVPQIIAGKDPMLVSLLGAFFFLAVSQYLVFGWTLKTHIALAGITFAIFVT
                     GLLSLLFVNWARLNGAGDESAMYLVQMGNGLDIKKLLVAGIFIGTLGVMDDLVIGQTS
                     AVIELYRGNPHMGFLQRFKSAMQIGQDHVSATVNTLILAYLGATLSMIVLYTMSSVDV
                     SVLVNINYVAEEIVRSLVGTIGLFLAIPFTTLVACWVVADESRLEKLVHIFGPLININ
                     ASTNDHVH"
     gene            15862..17778
                     /locus_tag="PLL88_06450"
     CDS             15862..17778
                     /locus_tag="PLL88_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOJ01245.1"
                     /translation="MKKITYFFLLLGLLLVLPACAGNIVVNSTSDEESAGAWVVEQSA
                     TAAAENRATTEAGTALGEYDADDLDASLDDPAMALIRLNGDTISFEGSGATVNGSTVT
                     ITSPGVYSVSGTLNDGQIVVDSAEDGTVRLILNGVNISNSSSAPIYVANAGKVVITLA
                     DGMHSYVSDASTYVYASADVTEPNAAIFSNDDLTMNGNGTLTVNANYNNGIATDDDLK
                     IVSGTILVKAVHDGIKGKDSIAIRDGTITVNAGSDGLQADNAEDVEKGTISIEGGTLN
                     IIAGLDGIQAETTLAVYAGNLTISSGGGSANGISKNGSQNDRGLQNNQNAVSSTESMK
                     GLKAGIAIHILGGVINIDSADDSLNANGSITVDDGVLNLTSGDDGVHANTALTINAGT
                     LTINQSYEGIESAVITINGGNLRITSSDDAINVAGGNDQSALGGRPGQNDFTANANNY
                     LYINDGYIYLDAGGDGLDSNGSIDMNGGTVIVNGPTSDGNGALDYMGSFTLDGGFLVA
                     VGSAGMAMTPTADSTQHSLLVNFTSQVAAGTMLHFESSDGTAIVTFIPTKNYQSILIS
                     SPLIQDGMTVDLYYGGTHTGTLTDGLYSGGAYSGGTLVTSLDITSVITGSGMGGGMLP
                     GGGGMPGGAGGRGR"
     gene            complement(17891..18067)
                     /locus_tag="PLL88_06455"
     CDS             complement(17891..18067)
                     /locus_tag="PLL88_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01246.1"
                     /translation="MESSFTGAQDDVGKRVALLQYVPEEEWKWRDSSVALLSRNDTFP
                     ALSIGKEAAMWCID"
BASE COUNT         4444 a         4209 c         4814 g         4807 t
ORIGIN      
        1 gtctctttct gcttatgccc gtcaattcct ggggcagatg agcaaacccg atgtggatta
       61 tatcgaaggg ctttcaccag cggtctccat tgaccagaaa gccacatccc acaatccccg
      121 ctccactgtt ggcactgtca ccgagatcta tgattatttg cgcttgcttt atgcgcgcat
      181 tggcattccc cattgccctg tttgcgggaa ggaagtggtg aagcaatctg ctcaggagat
      241 agtggatatc gttgcggcat tgccagccga taccaaattt ttcgtgttgg cgcctgtggt
      301 gagggggaga aaaggaacct atcaggcagt cttcgaggaa atccaaaaag ctggttttgt
      361 gcgtgcccgg gtcgatggac aggttgtcaa tcttgaagaa gatattgtac tggaccgcta
      421 caagattcat gatatcgaag cggtggtgga tcgattggtc attcgaaaag ctgagtcgga
      481 ggatgaccgg agcacttttc tttcccgcct gtccgattcc attgaaactg ccctcaaggt
      541 gggggagggc tatctggtgg ttcaggtgct ttctgatgag gggtcgcgcg atatgttctt
      601 ctctgaacac ctggcttgct ccgagcatgg cgtatctctc cctgccatcg aaccgcgcag
      661 cttttcgttc aatacccccc acggcgcttg tccggtctgt cagggtttgg gcgtgcagca
      721 ggagatcgat gtcaacttgc tcattcccga tcgcgaactt tccctccgcg aaggtgcgct
      781 ggtgcccagc gaatggaacg gaccgcgtga agagggcggg tattactggc agatgcttga
      841 ggctgctgcc agggaatata aaatcgatct cgataaaccg gtcggtgaac ttcctgaaga
      901 aaaactgaat gtgatcttgt atggaaccca gggcaaagaa gtgcgcatga atttcagccg
      961 cgaaaaccgc atctacgaag tagattctgc ttatgagggt gtgatcccca acctgatgcg
     1021 ccgcttcaag gaaacccagt ctgactacat ccgcaataag attgccacct acatgagcga
     1081 caaaccctgc accgagtgca atggtgcccg cctgcgcaag gaatcgctgg caatcaccgt
     1141 ggatggggtc aatattctgc aagtgaccga atggccggtc attcgcacgc ttgagtggat
     1201 caaccgcctg acttctgaga attctccctt atccaacaag gagaagatca tctctgatcg
     1261 tatcttgaaa gaaatcaacg atcgcctggg atttctggtg aatgtgggtc tggattacct
     1321 taccttgcag cgttcggctg ccaccctttc gggtggtgaa gcccagcgca tccgcctggc
     1381 aacccagatc ggttccagtt tgatgggtgt tttgtatgtt ctggatgaac catccatcgg
     1441 tctgcacccg cgggataatg ccaggttatt gaagaccctc atggacctgc gtgatatcgg
     1501 caatactgtg ctcatcgtgg aacatgacga agagacgatc cgttcggctg attggatcat
     1561 tgatcttggt cctgccgccg gagaaaatgg tggcgagttg gtggctgaag ggacggtgaa
     1621 ggacattctc gctcacccca gttcgctaac gggcgcctat ctttcccgcc gcaaggtggt
     1681 cccccttccc aaacaacgtc gcaacggcaa tgggcatgcg ttgaccgtgg tcggtgccag
     1741 gcaaaataat ttgaagaaca ttgatgtcag cattcctctc ggcaggctgg tgtgcatcac
     1801 gggcgtttcc ggttcgggta agtccacttt gatggtggat atcctctata acgccctggc
     1861 gcgcaagctg aaccgcgcgc agaccaatcc tggtctgtac gagcgcattg atggcacaga
     1921 atatcttgat aagatcatca acatcgatca atctcccatc gggcgcaccc cacgctctaa
     1981 cccgggcacc tacaccggcg tgttcgatga tatccgcaac ctgttcgccg aactgcccga
     2041 gagcaagatg cgcggctacc gcgcaagcag attttcattt aacgtgaaag gtgggcgttg
     2101 cgaggcgtgt ggtgggaatg gacagatccg catcgagatg caattcttgc ctgacgtgtt
     2161 cgttccttgc gaggtctgcc atggtaaccg cttcaaccgt gaaacgttgc aggtgaaata
     2221 caagggcaag aatattgctg aagtgatgga tatgaccatc gattcagcca tcgatttttt
     2281 tagcaatatt cctcgtatcg ttggaagatt gaagaccctt caagaggttg gcttgggtta
     2341 tgtgcgcatc gggcaatctg ccactacgct ttccggtgga gaagcccaac gcgtgaagct
     2401 ctcgcgcgag ctttcccgcc gcgccaccgg aaagaccctc tatgtgctgg atgaaccatc
     2461 cgttggtttg catgcggcgg atgtgcaccg gctggtggat gtgctgcaag cgttggtgaa
     2521 tgcggggaat tctgtgctga ttattgaaca caatcttgat atcatcaaga tcgctgatta
     2581 catcatcgat atgggaccgg aaggtggaga ccggggtggg gaggtgatcg cccagggcac
     2641 tcctgaagaa gtttgcgctt ccgctcattc ctataccggc gaagtgctga aagaatatat
     2701 tcaggatttt tacgcacaga aatagaacgt ccgggggttg gttttaataa aaaaaggcag
     2761 tgatcatctc actgcctttt tgggtatctg aattatttca gaatttcgac ttcttcagct
     2821 tgtgcacctt tttgaccttg tgtcacaacg aattgcacgc gctgtccttc ttccagattg
     2881 cggaaaccat ttccaacaat cgcactgtaa tggacgaaaa catcttcgcc cgattctcgt
     2941 tcaatgaagc catacccttt ggatccgttg aaccatttga cggtgccttg ctctttttcg
     3001 gacatgattt ggaactcctt tctataatat tgattgcttg ggttactcag gatttccaaa
     3061 tcaaaagagg atctttctat ttagaacctt actataactt acacaggggt taatcctatc
     3121 acaatttcat caaaatgggt tagcagttgg gtcgcagaga agcccccaaa agttcaaaaa
     3181 acccgattgt ccccttgaca aaaatcgccc aggaatgcta ttatatcgtg caacgataca
     3241 tagagaaacg atataacgaa gaatgatata aggaggcgcg atgacgagag caaaagagat
     3301 tgagaatatc ttgccgttga ctgagacctc tttctacatc ttgctgtctt tgatccaacc
     3361 attgcatggt tacggcatta tcaagaagat cgaagaaatg accgctgggc gtatccttat
     3421 gggggcgggt accctgtatg gggcgcttaa aaatttgctg gaaaatggct tgatcgtgga
     3481 agtagaaagc agttctgatc gccgccgtga ttaccagatc acgctcaaag ggaaagacct
     3541 ggtgctccag gaactgattc gctatgagga attgatcaag aatgcccgca gtgttgtggc
     3601 aggttgatgg gagaatctat atggaaaaaa agaagatcac gaagctgttt tttgcctggc
     3661 aggatgaaaa cgaacaaacc tggttagagc agatggctgc ccacggttgg atactggaag
     3721 aggttggctt cattcggtac accttccgca aagaaaaacc ccgtcatatc aactaccggc
     3781 tcgattacca ggaattaaaa gaaagtgaac ttcagagtta tctggagctg ttcgccgatg
     3841 cgggttggga atatatcggt cgtttgaata actggtttta ttttgcttca agtggtccag
     3901 atactcgaga aatctacacc gatgtgcact cgaagatcca aaaatataag agagtgcttc
     3961 gctctctgat ctttgcctgt atccccataa gctatcttct catcttcttt cctcgtttta
     4021 ctggacaagc cacgttctcc gatatcctgt tgttcatggt tttcttgatg gctctgatgg
     4081 tggttttcaa tataattcgc ctggcgctca gggtgaataa aatggaaaaa ggcttgtgaa
     4141 cgtctttccg gttgaacgcc gcacaggttt tggaacccca ccttaagacg gaggaaagtt
     4201 ccttccagag aagagtcgca gaactgatcg gtttggtata tagtattacc aatggattct
     4261 aagcaggatg gtaacgctac gatgacttct ctgaaaaaag ttttcgtggg tatcaaatgg
     4321 aagctgacca tcacctacat gctcgccacg cttctgatgt tatttttagt ggaggtgctg
     4381 attgccattt cgggcaaccg ttatgcgttc acgtcgctca attttgttta cagttccgca
     4441 caatatctca gcgaatcaac cgcttcgctt gcgcaggctt tgcgggaacc ggtggatgcc
     4501 gattttctga acgcttggat cacagacaat gaagagacct tcttacgagt gtctgataag
     4561 gatggtcgct ttgatcggaa aaatgatcct cagggaaaca cccctccgct tgacgacggt
     4621 tccattgtcg acttgacgcc caaaactgtc gctttcagca cctcaaagag cacattgacc
     4681 atcgtggatg ctaatggcat tatcctggtt accaacaacc ccctcatctt cccgccgggt
     4741 caatctatct ctgcctacct ctcggcagat gagaatacca tgcttgctac cgtggcatct
     4801 gccgttattc ccgctccgca attttcgatc atggagcaag acgcgactac cttgctgctt
     4861 cccatccgcc agggcgatca atacgtggga gccattcttg ctcaatatta tgcgcccact
     4921 ttttgggaac aggtgaaagc tgctgtttcc ggtttcttgc ctgaattgcc cgtgttcttc
     4981 atcatctctt cggcgatcgg tatcttgttt gggtttatca tcgccagcag tttcacccgg
     5041 cgccttgatc acatggaaga gacgaccctt cagtgggggc tgggtgattt ctctaagaaa
     5101 gccgaggtgc gcggaagcga tgagatcagc accctggctg tcagtttgaa tcacatggca
     5161 gaccagttcc agcagttgtt ggagaaggag cagatgttgg ctactatgga agagcgtaac
     5221 cgcctggctc gcgatctgca tgacagtgtg aagcagcaaa tttttgccat ttccatgaat
     5281 ttgggagcga tacgatcgct tttgaagacc aatctgaaaa aagccaccca acaggtagag
     5341 atctcttcaa agcttgccca gcaggctctg gacgaactga cctctcttat caacaccctc
     5401 cgcccggttc agttggaaga tcaatcgctg caagatgcct tgcacgatta tttgaaggtc
     5461 tgggaaaaac aaagcggcgt ctcggttgtc ttccgcaccg aaggtgccca ccagcctctc
     5521 gcccgcgaag ccgagcagtc ccttttccgc atcgttcagg aagcgctttc caatatcacc
     5581 cgtcacagcc gggcttctgc cgccagcatt tccttgactt tcgaagctga tcatttgacc
     5641 ttgcagatta gcgataatgg ggtcggtttt gataaaaacc tggtgaaaca gggtttggga
     5701 ttgcactcca tggaagaacg ggtaaaagaa atgcagggaa tcattaaatt gaattcttca
     5761 accgctggga cgaccatcac cattgttatc ccacgtgtga gggaggagca ggaaaaatga
     5821 cgaacgataa gatcacgatc atgctcgtgg acgaccatgc cattgtgcgt agtggcattc
     5881 gagcttattt ggaagtgttg ccagatatcc agatcatcgc cgaagccgaa tcgggcaccc
     5941 aggcggtcaa gcttgccgaa cagcaggcac ccgatgtggt gctcatggat ctgaaaatgc
     6001 ctgagatgga tggtgtggag gcgacctggc gcgtacggca ggtgagtccg cgctcacaga
     6061 tcatcattct cacttcctat catgaagatt cgcatatttt ccccgctatc aaagccggcg
     6121 ctttgtctta tattctcaag actgtcggac cggaggaatt ggcgaatgcc atacgttcag
     6181 cggcgcgtgg cgaagtcacc ctttccccca aggttgcctc gcggctcatc ggtgagtggc
     6241 gtgatgatcg cagtgatggc atgcaagatt atatgcagct cacggaacgc gaacaggaag
     6301 tgctggctct tatcgccgag ggcttgtcga atgctgagat tgccaacagg ttggtgatca
     6361 gcgaaaagac cgtcaaaagc catgtcagca acatcttgag caaattgcat cttgccgatc
     6421 gcacccaggc ggcggtgttc gcctggcgcg aagggatcat caaaaaagag aacacataaa
     6481 cgctgtgtcc tcctctttgt tctgttgatg cactggcttc tacttgcgcg gcatctctcg
     6541 cgcaagggga atgattggat tccaggcgat ccctttttgg actgcacgca gttcgctttc
     6601 taacattaag cgtttgatct ccagcccgac ctggttggtg aattccttca ccagttctct
     6661 gttttctaca tctccttcga accgtagcgg tttgccgatg gtgatgtaat atttcccatg
     6721 cagcagccag cgcgattcct ccaacccata ttttcgatgg aaaaagtgcc gccggatgta
     6781 tgtcgggttg acgtaaatgc ctaccggcac gatggtagcg tgcgctgcga gcgcgatgcg
     6841 ggtgacaccc gtatgcagct cggagagcat gttcaccgaa ggtgtcaggc acccttcggg
     6901 aaataccgcg atcggcaggt cttgttcgag cacctgcagg gagcgctgaa tgatctcttg
     6961 gctgttttta ttttctttat caactggtat tgtgccggct tgttcaatta atctcccgat
     7021 aatggatatc tgcatgatcc ttttgttgac cagcaccgtt agaggttctt cggtcaccag
     7081 gctcatcaga aaaggatcgg ttgtggtggg gtggttggca gcgaagatct ttgctcctcg
     7141 cttcagaggg gcttcataat ggacgttaaa ttccaggaat aatctggcaa agatattcag
     7201 gatagttcgt ccgatgctca ggagcagtct ttttttccag ttcattgcgt tccctttgca
     7261 gtgtaatttc cccaaattct attggtggtt tttcctttgt tccagtcctg atggtaaggg
     7321 aaaccaggtg aactggtgta ttatttatat tactcatgac tggatagaaa tgactcaagc
     7381 aatatggtag aaaggtgatt gagaatataa tctacaacaa tgaaaagtaa acgtatgctc
     7441 accagccgtt ttccatacgc cctctcgctt tttgcggtgc ttacctttgc ctgcatcagc
     7501 gctttggatt attttatttt tcaaggggaa ctgaccaaag ccgacccgca ttggaagaaa
     7561 tgggtgctct tttccagcta tttcttatta gctgcttttg atctcttcga tatgctcttc
     7621 tattcgaaga ctcgtttgtt catgcatacc ctcttctttg tattgcgcat cattgtgatt
     7681 ggtttcatga acagcatcga tatcacccat ttttcgcgca tactctttgg tctcatccct
     7741 tatcatgctt acctgatctt tggactggtg atcagcctgt tatccatgat tgtcgtgttg
     7801 tattactttt attccatttc cgcctttatc ccaactcctg aaacgcttcc ggctttagcc
     7861 agcgttttgt ttttccagct tacagccatc tttgccgaaa gggataagaa ggctaccttt
     7921 gagaaccagg agatcatcga agagctttcg cgctccaata tcgaattgcg tacttatatc
     7981 gaggatttat cgcgcctgtc catcatggaa gagcgggtgc agctatcgaa tgatctgcac
     8041 gattccattg ggcattatct ggtggcgatc aatattcaac tcgaaaaagc gctcgcctat
     8101 cgttccattt cctccgagac tgtagatgaa gccatcatca acgccaagtt ttcggtcgag
     8161 caggctttgc gggaggtgcg ccgctttgtg gatggcttgc acgaccacga ggatatttat
     8221 agtctgaaag agaaggcaca ggaattgatc accaatttca aggatgtgtt cacgactctt
     8281 gatttccaat atgaggggaa cgaatttgct ttcgccaaga tcatccgacg caacctgttc
     8341 tacattctcc aggagtcgct caccaatatc caaaaacatg cccacgcgca aaatgtggtg
     8401 gtgaccatca atttcaaggc tcgagaggtc gtcatgctgg tgaaggatga tggtgttggt
     8461 ttcaaagcga agaaatcggc tgaccaggca gggcattatg gtttatccag cattcaccag
     8521 cgggttgaat tgttgaatgg gtcggtcaat attgccagca ataagaagaa gggtaccagc
     8581 atcgaggttc ggataccctt aaaacaggaa aacccgcaga gagggaaaga ccatgaatga
     8641 taagatcagg gttttgattg tggatgacca gcagttggtg cgcgatggca tcaaagcctt
     8701 gctggaaata aaagaagatt tctccatcat cggcaccgcc gccgatgggc aggaagctat
     8761 ggatgttctc caacagaccc cggctgatgt ggttctcatg gatatccgta tgcccggttt
     8821 gggaggcatc gaagccaccc gccagattcg ccaactgaat aaggaaatcc gtatcatcat
     8881 gctgaccact ttcaacaatg atgattacat cgtaaaagcc atcaaggcgg gggcgaatgg
     8941 ctatctgttg aaggatattc ccaaagatga cctggctgaa gccattcgtc tggcttaccg
     9001 aggggtttcc caattctctt ctgacaccat cggcatgctg gtagaacgca atctgctgcg
     9061 cgataccgat gtttctccag aaccggagca ggaactatcg ttgctcaatg aaaaggaata
     9121 tgagatcctg tgtatgatcg cagaagggta ttccaatcgg gagatagcag aagagctctt
     9181 ttttacggag ggcacggtca agaactatgt ttccaagatc ttgaacaccc ttcatctgcg
     9241 cgaccgcgtg caggcagcca tttacgccgt tcaacatcat ctgcagagga aatgaaaagg
     9301 caggcagccc tccagttgga agaactgccc ctggcaaggg tttttcggac gctcgtgaaa
     9361 ttttccatag ttttcctgcg tgcggtgcgt ccatctgtgt gctcaagtct ttctcactgt
     9421 aaaaggaaaa agcctgttta tcgctcattc ttcacatctt cgtagtagat cttgatctcg
     9481 gcggtatcgt tgaggaagtt gatcttgttg atgtccaggt agtggatgat caaccccgtc
     9541 cgttcttcca ggtctgcgat cagttccttt tggttgttgg cgttgaccag atcgattttc
     9601 tcataggtga ttgatttgga agccagaaag tggaagcccc atcccttttc caaaaagacc
     9661 agcaccgcgc tgatcaacag agtggttaat cccacttcta tgtaagcgcc ctggctgaac
     9721 atgatgtaat tgaacattgg cagcgcgatc aggatgaata aataggtcat ttcgcgcgat
     9781 ggcatgggat cagtgcggta gcgcaacagc gagaagatcg cgaacagtcc gaaccctacc
     9841 cccatgctga gttcgatgct tgccatatag ttgaacagga tgaaaatgat ggtattgaac
     9901 cccagaaagg tcagcacgaa tttctgagaa cgctgctgcg ggtaatagat gaaccgcacg
     9961 atcacgaagg cggttaggaa attaaaaaga aagtgaagta tatactggag atatgttgcc
    10021 atttttgtag atcctttttg ttgaagcttt gaattgcaaa aaattttgtt tgaacctgtt
    10081 acgtttgatg tcggggttca gaaaagccac gccggtgcaa tatttgctga acgacatggg
    10141 ttggtatcct ttttctctca atgcttgcag gatgacggat tctcttgaat agttgtcttg
    10201 tttcacttcc accacagcca ggtgtgataa ttgcaggctg ttggttctgt ttgaaaaact
    10261 caaccccgtg tcaatggttg cccgctcgca agcattgagg ttgaccaggg tgatcctctt
    10321 aaagttgttc cgcaggaccg ctttcagatt ccgcctgtct tctgggtaat gctgtgccag
    10381 gaatgcgcac atctctgcgc tgaacgatgg tctgatgcgc gcgatcggca tgcggttctt
    10441 caccgtgcgc aactcgttct ctcttttctt gacctccaga aacgtgtcgt ttgtggagtg
    10501 gtattctcgc atgcggatct tgtagcgcgg gcgttttcca ttgtgatggt caaagtagaa
    10561 ggtcaagcat ggggtgtcaa aatacaacgt gcggtacgcg ctgattcgtt tgttctccac
    10621 ttctaagatc gaataactgc ctggcaaaat ggacagcagg gagggcacat ccactattct
    10681 caccaggaac ttgacgtcaa cgcggtcttg cagcgcgtat tcctctatct catgcaatga
    10741 cacggtggaa taggtcggca cgatctcttc gatggctgcc atggtgcggt tgaatagcga
    10801 aggcagcaac agggaatagg tttgattttg ggtggtaaac atggtgcaac ctccattcat
    10861 gcccttatca tagaatgtcg cctgcggaat gatatccacc atgggatgga attcgattca
    10921 gcctgtggag gtatttgctg tataggtagc atggtgaaat gtttcgtgcg aagtgcgctg
    10981 gcagccattg ccgtcctgtc catgagcggc gggggaagat tggcttgaca gataaaaaga
    11041 attagtatat gctaacctca atatttagat atatataata gatatataaa aaaggaggat
    11101 gcatgaccag tttagacatc gtggctccgg gtagtacctg tgtggttcaa gccatcgagg
    11161 gggagggggc agtgcgtacc agaatgatgg atatgggttt gctgaaagga acggtagtaa
    11221 aagtgatacg ggttgctccc ctgggagacc caatcgaatt cgaagtgaaa ggatacaacc
    11281 tttcattgcg aaaaaaagaa gcgcgcatgg tggctgttca ggtaatggat gatgacgctt
    11341 agtattgctc ctgaagggca gcttctcacg ctcgtttcta tcaaaggtgg attgggggtg
    11401 caaaaacgcc tcaatgcgct tgggctcacc cccggcacag aagtgatgat cgtctcacag
    11461 aatgagggtg gaccgatcat tcttgcagtg aaagattctc gattggcaat cgggaggggt
    11521 ttgctacacc atattcaggt agaagcaaaa gaatcttcgc tgcatcaaac cgaaccgctt
    11581 gatcctcaga tctcatcata tcggcatgtt cacgatagga ggcaccgccg ttttggaggt
    11641 cgtcatggat gatcgagaaa ataaggaaga aatcatggaa aaggaattaa tcatcgcagt
    11701 agctggaaat ccaaattgtg gaaaatcaac ggtttttaat cagcttaccg gtacgcggca
    11761 gcatgtgggc aactggcccg gaaaaaccgt tgaaaagaag gagggtagtt ttcagtataa
    11821 agggcagcac attaccgtcg tagatctgcc aggcacctac agtttgaccg catactcact
    11881 ggaagaagtg attgccagag attatgtcat taaagaaaaa cctgacctgg tggtttcgat
    11941 tattgacgct gccaatatcg aacggaatct ttatcttacc atgcagctca ttgaactcgg
    12001 ggttccggtg attgctgcac tgaatatgat ggatgtggtg gcagaacgcg agattcagat
    12061 tgatgccgag aagattgaaa aggctctgcg cgttcctgtg gtccgcatgg ttgctcgcga
    12121 tggaaaggga ttggatgagc tgaaggaaaa gatccttctg attgcaaaaa cgaaacagga
    12181 atataaaaaa gaggtcccag ctcgcttcaa caaagaatta gaagaagcga ttcaaaaact
    12241 tcaggtgaag atcgagagta tgccaaaaga caagcgctct ttcgattctc gctggttggc
    12301 gattaagttg ctggagggcg ataaaggagt tctgaacgac ctgaaagaag aaaagaatgg
    12361 ctacaagagt ttaatagaag aagctcgcct cttttctcag acctatgaag aaaggtcaga
    12421 ttttggaatt gatacggaaa ttgccgatac ctattacagt acgattcata ctatcgtcaa
    12481 tgcgtctgtg aaaaagccca agatacagaa agccagtttt tctgataagc tggatcgtat
    12541 cgtcacccac cgtgtttggg gcatccccat cttcttaggg atgatgtgga ttgtgttcaa
    12601 gttgaccagt gatgtggcag ctccttttct ggattgggtg gatggcgtga tcggcggtcc
    12661 gcttacccgt tgggtggtct ctctgctcac attgctgagg ttggacggca cctgggttga
    12721 atcgatgttg gtcgatggcg tgattgcagg tgtgggaggg gtgttggtat ttgtgcctgt
    12781 gatgatgatg ctgtacctgg ctctggcaat cctggaagat tcggggtata tggcaagggc
    12841 tgcttttgtc atggatcgcc tgatgcatgg tcttggcttg catggaaaaa gctttcttcc
    12901 catgattgtt ggctttggct gcacggtgcc ggctatctat gccacgcgca cgttggaaaa
    12961 tgaaaaagat cggattctga ccagtttgtt ggttccgttt atgagttgtg gcgcgcggct
    13021 gccggtgtat gtgctctttg ccaccatttt cttccctcaa aatactgggc tggtcatttt
    13081 ctctctgtat gtgatcggga tcgtgatggc tatactggtt ggattgctgt tgaaaaatac
    13141 ggttttcaaa gaacgggatg tatctccttt tgtgatggaa ctgcctccct accggcgacc
    13201 taccctgaag agcatttggg cgcagatggc tgaacgcacc aatgctttcg tgaaaaaagc
    13261 caggggattg atcctggtga ccagtatctg tatttgggcg ctgctggctc tgccggtaaa
    13321 aggagaaggt cgctttgcgg atgctccggt tagcgatagc gccttcgctg cggtctcttc
    13381 ggcagttgcg cccatcttta ccccagccgg ttttggaaca tgggaagcat ccggttcgct
    13441 gttgaccggc tttgtcgcaa aagaggtggt tgtcagcacc atgtcacaga tctatgaggc
    13501 gcaagaagcg gaagaagaag ccgtagcgac cacatttttg gaagatattt gggagattgt
    13561 ttcatcattc tttgtggctg cctgggatac cataaaatcc atcccgttga tcgttggaat
    13621 caatctcttt aatgaggggg aagaagctga accaacggtt ttgatggcgg ctgttcatga
    13681 tggctttgaa gcctcaagcg gagggagagg agcgctggca tcctttgctt tcatggtctt
    13741 tgtcttgctg tatacgcctt gtatggtggc aattgcggca gagaggcatg aaattggaac
    13801 gaaatggatg tgggtcagtg tgatcggtca attcgctttg gcgtggcttg tatcgacatt
    13861 ggtcttccag atcggctctc tcctgtgacc tttgatgacc aggcaggtcc tcgttcaaac
    13921 ctgcctggtc tctggtaatc tgttaggtga aaaatgattc aaagaatcct tgaagaaatc
    13981 aataaaaatc gtggttttgt cagtttgaat gaacttgctg aaaggttgga gttggacccc
    14041 aacgtgttat caggcattat gcaaaccctc aatagaaacc gggatctgaa agtggaaact
    14101 ggcatgcctg attgttcgca cgcttcctgc cccgcttgct cggtgaagaa catgccttcc
    14161 gagatgattt gcggttgtca ggtcaaaccc aagcgttctc agagtgatcg cctttcctga
    14221 aagaaaaata cattaacctt ttctgttcag tggtcaatgc ttcgattgcc cgctggaagg
    14281 atataattct ctgttcatga attgaaaaaa acgacggaga atgaaaatgt ggcattggtt
    14341 caatcgaaat catagatgga tcacggcgct ggtgggtgtg gtcgtggtgg tcgttctgat
    14401 tctcttgtgg cagacgagtg caaataatca aaatgtggtc ggttactcct ctactactgt
    14461 gaaggcgcgc gttaccggca tacttgagga aggcacggtg accatggggg agaagacgca
    14521 aacctaccag gtgctttccg tgaaattgtt ggaaagcgac caggaggggc aggtgtatga
    14581 actgcagtat ggaaagtacc agttgctgtc ggatgattat cgtttacagg tgggcgataa
    14641 gattttggtc aatttggaaa gcatgcaaga tggtacctat gctgtatatt tcatcgatca
    14701 cgtacgtact gactcattgc tggttttgct ttgtatcttt attgtcaccg gcattgctat
    14761 gagcggttgg aaaggcttga acagcatgat cagtatggca ttcagcatcc tggttatggc
    14821 tgtttatgtc gtcccgcaga tcatcgcagg aaaagaccct atgctcgtca gcctgctggg
    14881 agctttcttc tttttggcgg tatctcagta tctggttttt ggttggacgc tgaaaaccca
    14941 cattgccttg gcaggcatta ccttcgccat ttttgtcacg gggctgctct cgttgttgtt
    15001 cgtgaattgg gccaggctga atggagccgg agatgaaagc gccatgtatc tggtccagat
    15061 ggggaatggt ctcgatatca aaaaattgtt ggtggcgggt attttcattg gtacgctggg
    15121 tgtcatggat gaccttgtga tcggtcagac ttcagcggtg attgaattgt atcgagggaa
    15181 tcctcacatg ggctttcttc agcgtttcaa gagtgccatg caaatcggtc aggaccatgt
    15241 aagcgctacg gtgaatacgc tcattctggc ttacctcggc gccacgttga gcatgatcgt
    15301 gctgtatacc atgagcagtg tcgatgtgag cgtgctggtg aacatcaatt acgtggctga
    15361 agagatcgtt cgctccctgg tgggcactat cgggcttttt ctggcaattc ctttcaccac
    15421 tcttgtggct tgttgggtcg ttgcagatga aagcaggctg gagaagctgg tgcacatttt
    15481 cggacctctc atcaatatca atgcttccac gaatgatcat gtacactgat tcgggcattc
    15541 attgaaggcg ataccattcg ggtggaaccc ggtcaggatg ggctgaagct cagggcgcat
    15601 catgaaaaga agaaggaata gttttaccga tcgagaggat tcgtttcaga aatacggatc
    15661 ctcttttgtt ttaaaggagt cgtaatagat accccgccag attgaaaaca tttccgcctt
    15721 ttgatggatg gtagaatcac cactgccctt tatcatgggt tcacactttc atccgatgca
    15781 tggttgattt tctttgcgtc gaaaaagaaa cagatagaaa cacagatgaa ggaaacattg
    15841 aaaaacgata gaggttgaca agtgaaaaag atcacatatt tcttcctgct gctggggctc
    15901 ctgcttgttc tgcccgcttg tgcgggaaac atcgtcgtga atagcacgtc tgatgaagaa
    15961 tccgcaggtg cttgggttgt tgaacaatca gcaactgccg cggcagaaaa tcgggcaacg
    16021 acagaagcgg gtaccgctct gggggagtac gatgccgatg acctggatgc cagcttggat
    16081 gatcctgcca tggcgttgat cagattgaac ggtgacacca tttcctttga aggcagtggc
    16141 gcgaccgtga atggcagtac cgtcaccatt acctcccccg gtgtctacag cgtcagtggg
    16201 accctgaatg acgggcagat cgtggtggat tcggcggaag atggcacggt tcggcttatt
    16261 ttaaacggag tcaacatcag caattccagc agcgcgccca tctatgttgc caatgccggc
    16321 aaggtggtta tcaccctggc ggatggcatg catagctatg tgagcgatgc cagcacgtat
    16381 gtctatgcca gtgcagatgt caccgagccc aacgcggcta ttttcagcaa tgatgatctg
    16441 accatgaatg gcaatggcac gcttacggtg aatgccaatt acaataatgg gatcgccacc
    16501 gacgatgacc tcaagatcgt cagcggtacc attttggtga aagcggttca cgatggcata
    16561 aaaggcaaag attccattgc gatccgcgat ggcaccatca cggtcaatgc aggtagcgat
    16621 gggcttcaag ctgataatgc cgaagatgtg gaaaaaggca ccatctccat cgagggcggt
    16681 acgctcaata ttattgccgg gttggacggc attcaggcgg aaactaccct tgccgtctat
    16741 gccggaaatc tcaccatttc ctcgggcggt ggcagcgcga atggtatcag caaaaacggt
    16801 agccagaatg atcgtggtct gcagaataac cagaacgctg tcagcagtac ggaaagcatg
    16861 aaagggctca aggccggtat cgccatccat atcctgggtg gtgtgatcaa tatcgattca
    16921 gccgatgatt ccctcaacgc gaatggcagt atcaccgtcg atgatggtgt attgaacctg
    16981 acctccggag atgatggcgt gcatgccaat actgccctga ccatcaacgc gggaacgctt
    17041 accatcaacc aatcctacga aggtatcgag agtgcggtca ttaccatcaa cggcggaaac
    17101 ctgcgcatta cctctagcga tgacgccatt aatgtggcgg gtggaaatga ccaatcagca
    17161 ctgggggggc gcccggggca gaacgatttc accgccaatg ccaataacta tctgtatatc
    17221 aatgatgggt acatatatct ggatgcaggg ggtgatggtc tcgattctaa cggctcaatc
    17281 gatatgaacg gcggtacggt catcgtcaat ggtcccacca gtgacggcaa cggtgcgctg
    17341 gattacatgg gatccttcac cctggatggc ggttttctgg tggcggtggg tagtgccggc
    17401 atggcaatga cccccaccgc tgattccacc cagcactctc tgctggtcaa tttcacctct
    17461 caggttgcgg cggggactat gctgcacttt gaaagctcgg atggaacggc gatcgttacg
    17521 ttcatcccta ccaagaacta ccagtccatt ctcatttcct ctccgctgat ccaggatggc
    17581 atgaccgtgg atctctatta tggcggcacg cacaccggca cattaaccga tggcttatat
    17641 agtggtggcg cttacagcgg cggcactttg gtcaccagcc tggatatcac cagcgtgatc
    17701 acgggttctg gcatgggcgg tggcatgctg cccggtgggg gaggtatgcc gggcggcgct
    17761 ggcggcagag gtcgctaagc attgaaaaga aaggggcgcg gaatttttcc gagcccttta
    17821 aagagtattg ctagaacaga aataagaatt tatgaagtgt gtgatatgaa tgattgggat
    17881 gaaaggggga ttaatcaatg caccacatgg ctgcttcctt ccctattgat agggcaggaa
    17941 aggtgtcatt ccgagagagc agcgcgaccg aggaatctct ccatttccat tcttcttccg
    18001 gaacatattg tagcaacgca accctctttc caacgtcatc ctgagcgcca gtgaaagaac
    18061 tctccatttc tttatcaaac caaccaacct gctttatccc attttctctc cccaacgtca
    18121 ttcagaggta gcacagcgac cgaggaatct ctccatttcc attcttcttc tggagcatat
    18181 cgtagcaacg cagccctctt tccaacgtca tcctgagtct cgggtagata ccgtcttgaa
    18241 agtcaagaag caattgcaca tttgtgagtg aaat
//
LOCUS       DAOSDS010000043        18090 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1259_length_18090_cov_4.207375, whole genome shotgun sequence.
ACCESSION   DAOSDS010000043 DAOSDS010000000
VERSION     DAOSDS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18090
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1259_length_18090_cov_4.207375"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..471
                     /gene="rplQ"
                     /locus_tag="PLL88_06460"
     CDS             85..471
                     /gene="rplQ"
                     /locus_tag="PLL88_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOJ01247.1"
                     /translation="MRHQVAGKKLNRNSGNRKALRSTLIKQLFTYNKIKTTRAKAQAV
                     RSEAERLITVAKNSSKSTDAEKVHARRLVAAKLNNAATVKQLFDNIAPRFENRNGGYT
                     RVTKLGPRKGDDAEMVLLELVEDEKK"
     gene            496..1248
                     /gene="truA"
                     /locus_tag="PLL88_06465"
     CDS             496..1248
                     /gene="truA"
                     /locus_tag="PLL88_06465"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOJ01248.1"
                     /translation="MEHYQLIIAYDGTDYRGFQRQNKSGTIQGELESALRKIGWQGDT
                     IQAAGRTDRGVHATGQVVTFFFDWEHSLKDLQAAINSNLPPDIAIQSIQLVKDGFHPR
                     YDAVSREYTYAVTISDVRQPLKERTVWRIDRELNWSAMQATAKLFIGKHDFSQFGRAL
                     KDDGSTVREVLASNWEQKTAADYEYTICANAFLYHMVRRIVYVMVKVGQGKMAAQDIQ
                     RALGGEAVKIAPGIAPARGLTLTKVRFRDNSE"
     gene            1282..1707
                     /gene="rplM"
                     /locus_tag="PLL88_06470"
     CDS             1282..1707
                     /gene="rplM"
                     /locus_tag="PLL88_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOJ01249.1"
                     /translation="MEKTFVIKGKPETEWVLIDASGQSLGRLAGDIANILLGKNKATF
                     TPGVLMGDNVVVINAEKIVINPKRMEEKVFYTHSNYPGGLKAVNMPDMLKKHPERVLS
                     KAVWGMIPHNKFGRQVIKRLHVYAGNKHPHSGQDPKPVK"
     gene            1721..2116
                     /gene="rpsI"
                     /locus_tag="PLL88_06475"
     CDS             1721..2116
                     /gene="rpsI"
                     /locus_tag="PLL88_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOJ01250.1"
                     /translation="MSAQYYEGIGRRKTATARVRLSAGSGNFMINQKNLEEFFTRYGD
                     TETILAPFKATGENQAQYDVSVFISGGGVNGQTTAVQLGLARAFAKMNPDYVSVLRKN
                     RLLTRDPREKERKKPGLKRARKAPTYTKR"
     gene            2224..3315
                     /gene="mazG"
                     /locus_tag="PLL88_06480"
     CDS             2224..3315
                     /gene="mazG"
                     /locus_tag="PLL88_06480"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOJ01251.1"
                     /translation="MNTDLLNTVLESTKHHVTIIPASLLNGRHFPPFFSNQNALLTGF
                     VKKEDLVFAFELLAKNYPADHALLFFKQGADRKESFIKFEMTLQDVRSRGPQDGVIAV
                     LVPAKTDKNSFLDFLELIAHLRAPEGCPWDREQTHQSLRPNLLEETYEVLQTLDSHDM
                     IGLQEELGDLLLQIVLHAQIASENRDFTIEDVITGIERKLIFRHPHIFGDKAVSDANE
                     VIKNWEVLKAQERRANNKAQGLLRSVPKGMSALSLAQAYQKRAARVGFDWESIEPVKQ
                     KIDEELQEVEKADRDEERAKELGDVLFAMVNLIRWYGQDAESVLREAAEKFASRFEYI
                     EDCVQKSGKTFADFSLAELDAFWEDAKKR"
     gene            complement(3322..4701)
                     /locus_tag="PLL88_06485"
     CDS             complement(3322..4701)
                     /locus_tag="PLL88_06485"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOJ01252.1"
                     /translation="MQPHPPEPFRIKSVEPIRLISSEQRTKAMQEAGYNLFTLKAEDV
                     YIDLLTDSGTGAMSQNQWAAIMQGDESYAGAKSYYHLKQTMQEIFGFDYFVPTHQGRA
                     AENILSTILVKAGAIIPSNMHFDTTDANIRARGGDPTNCVIGDAFIVQSTNPFKGNMD
                     IVKLEELIRKVGPQRIPFGCITITNNAGGGQPVSLKNIRETSQLYHQYGIPFFIDCCR
                     YAENCYFIKKREPGYEEKSLMEIANEIFATADGAFMSAKKDAIVNIGGFLAVRDADLF
                     QKISNELILREGFITYGGLAGRDLDAMAVGLREGLDEAYLAYRIGQTAYLGSRLIENG
                     IPIIEPPGGHAIYVDAGSFLPHIPHAEFPGQALAAAFYLEGGIRGVEIGSVMFAHPDP
                     QTGEVIFPKLELVRLAIPRRVYTQSHLDYVVEILKKVGQHRQSIRGYRITYEPKLLRH
                     FTAKFAPLS"
     gene            4798..5439
                     /locus_tag="PLL88_06490"
     CDS             4798..5439
                     /locus_tag="PLL88_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01253.1"
                     /translation="MKILKHNTFLRFISGVIFFVSSMCLIVTSFEGPVVEKMYQFPES
                     FIENEVDPAWQESVTNALASYQINLRHPEWVWYGESFFIQVSLIERAGLPTSENNALP
                     SFVLEAYLEMNNVGTTPGEHILLPIQLPQTGLLQWEVDPQAEEIEQSRVWLSILPQEG
                     GDSSIPVLVLPLEIKIRPLLGLKLGGWQMIWIGLGVISIGFYLSDHLKRNRRV"
     gene            5735..7318
                     /locus_tag="PLL88_06495"
     CDS             5735..7318
                     /locus_tag="PLL88_06495"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOJ01254.1"
                     /translation="MKSSVTVREIFSSCPGILAAPTTLPQLPVAGVEFDSREVTKGSV
                     FVAIEGEYQDGHAYIRSALDRGAVMIAGTQQKWLEMLPDQYVWVDDGRKALAYLAAAY
                     YGFPARRMTVLGVTGTDGKTTTTNLIYQILKQAGFKTGMISTVNAVIGDETVDTGFHV
                     TTPEAPMVQKLLARMVNCGLTHVVLETTSHGLAQQRVAACEYDIGVFTNITHEHLDYH
                     GTYENYVLTKARLISFLAETKSKELGAIRLAVLNKDDQSYPFLQKILKGIAGDAVHSI
                     SYSRENSADICLLNEAHFPNGLSIELAIRDQKVSIHSNLIGSYNVSNIMAAVGATSIG
                     LDIAPEVISAGIAALPDIPGRMERIDLGQNFTAMVDFAHTPNAIKVTLETARKITKGR
                     IIAIFGSAGLRDREKRKLMAVEGVKQADLCIFTAEDPRTEKLEDILAEMKLAAEKVGG
                     ALGKDFFIEPDRGNAIRLGLQLAETDDLVVACGKGHEQSMCFEKTEYLWDDRIAMRAA
                     LAELLKIEGPPMPYLPTAK"
     gene            complement(7368..9563)
                     /locus_tag="PLL88_06500"
     CDS             complement(7368..9563)
                     /locus_tag="PLL88_06500"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOJ01255.1"
                     /translation="MQQKAFSYSGTIGDHEFTFETGKLAGQAGGAVTLRVGNSVLFAS
                     ATMSNTVIEGTDFFPLSVEYEEKMYAGGRIPGGFFRREGRPGDDAVLVARLTDRPLRP
                     LFDKNMRNEVQIMMFPISSDGVNPLDIMAINAASAALVISDVPWAGPVGAVRVGHIDG
                     KFVVDPTFEQMQISDLDLRIAGTKDAILMVECGANEVKENDMIAAISFGHTSIQSIIA
                     VQEKMAAEIGKTKRDVPLTNIDQALIEQVYEKGATKINDALDKPHSKAELDAAIDAIQ
                     EELLEEYANETDEDHAKLLHDVKEAFHDAYQKVVRTRILTKGIRPDGRDPKQIRPIWC
                     EVNVSPRAHGSGLFTRGETQVMTLATLGTPKEAQEIDDLTPTESKRYIHHYNFPPFST
                     GEVQRPHRSRREIGHGALAERALVPVLPAQSEFPYTMRLVSEVFSSNGSTSMASVCGS
                     TLALMDTGVPIKAPVAGIAMGLIKENEQYAVLTDIQGLEDHLGDMDFKVAGTTAGITA
                     LQMDIKIKGITPEIMSEALAQAREARLAILDKITEVIAKPRPELKEFVPRITTVKIPV
                     DKIGALIGPGGKNIRNLQEETRTQIDIAEDGTVFIAAVDKQDEMNARSQIEKMIEVPT
                     IGHIYTGKVTKVVDFGAFVEILPGTEGLVHISQLSVDRVKSVQDVVVEGDELSVMITE
                     IDPRSGKIRLSHVAVLEGWSVEEAREHDNHNNKSGERKSGSHSGKRTKR"
     gene            complement(9709..9981)
                     /gene="rpsO"
                     /locus_tag="PLL88_06505"
     CDS             complement(9709..9981)
                     /gene="rpsO"
                     /locus_tag="PLL88_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOJ01256.1"
                     /translation="MTMTKEEKQKVIESYKQHENDTGSTEVQVALLTNRINQLTEHLK
                     IHKHDESSRRGLLKLVGQRRSFLTYLKKKDYKKYVAITEKLNIRTK"
     gene            complement(10110..10898)
                     /locus_tag="PLL88_06510"
     CDS             complement(10110..10898)
                     /locus_tag="PLL88_06510"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOJ01257.1"
                     /translation="MPAVDLTVLNSRLQSLQLFRSDARIYLQKLEDLLSMYSSEKTKL
                     GETTPFQTLLRRLYVPDLVLEKIIETFPALTHDFLENAIDLLDTLWDREELEYKILAV
                     NLLLCLPVEKREEISKRLLQWVSSTDEELLHSVVLAQLPFSHKENSTSIQTFIEHLMH
                     MDDPEMRKLAFHILARVIEQKHFSDLPWVFNILAPFIEAASLQTYGQLNPVMTALIRK
                     SEIETIAFLYEICTHANSTKARKYVRKIIHFFTPENQKYLIEVL"
     gene            complement(10908..12341)
                     /gene="proS"
                     /locus_tag="PLL88_06515"
     CDS             complement(10908..12341)
                     /gene="proS"
                     /locus_tag="PLL88_06515"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOJ01258.1"
                     /translation="MANEKLTPRDDNFSDWYNEIIQKAEMADYAPVRGCMVIKPYGWA
                     LWENIQKALDKRFKDTGHQNAAFPLFIPMSFLEKEKEHVEGFSPELAVVTHGGGQDLE
                     EPLVVRPTSETIIGHMYAKWIKSYRDLPVLINQWANVVRWEMRTRLFLRTLEFYWQEG
                     HTAHATAQEAEEETLRMLHVYEDFAVNEAAIPVICGMKSETETFAGALHSYTIEAMMG
                     DTKALQSGTSHNLGQNFARAFDIQYLDDQNQLQYCWTTSWGLSTRFIGAIIMTHGDDQ
                     GLMLPPRLAPIQIVIVPIFHNLEEREAVMQYAQEIMTELSDFRVKLDDRFEQTPGFKF
                     NDWEMRGVPLRIEIGPRDVANNKLVIARRDIPGKAGKEVMDLDALHPRTAYLLDKIHS
                     SLLQRATSFRNSHIHEAATYDDLKKSVENGWAFSYWCEGKDCEVKIKEDTKATTRCIP
                     FHQDHTQGTCVVCGKETATKAYFAKAY"
     gene            complement(12316..12894)
                     /locus_tag="PLL88_06520"
     CDS             complement(12316..12894)
                     /locus_tag="PLL88_06520"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HOJ01259.1"
                     /translation="MKVSVFGSAHPKPGDTEYLEAYRLGKCLAERGYIVLNGGYVGTM
                     EAVSKGTAEAGGHVIGVTCDDIERWRNDKPNPWVHEVMHHTDLFGRISGLVRNCDAAI
                     ALPGGVGTLAEIALTWNMIILESIPVPYLLLVGEGWKRVMTTFFDEMPCFVPTSVREY
                     IRFCLNIEEAVAQLDNFYVEKMRKHGERKTNP"
     gene            complement(13031..15100)
                     /gene="fusA"
                     /locus_tag="PLL88_06525"
     CDS             complement(13031..15100)
                     /gene="fusA"
                     /locus_tag="PLL88_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ01260.1"
                     /translation="MKEYSTSNIRNIALVSHSSAGKTVLVEAFLHTTGATTRMGSINE
                     GTTVSDFDEEEVRRSISLYSSVIPVEFKDTKINFIDTPGYTDFVGEMISALSVVEGAV
                     IVVDAVSGYEVGTEVAWKNCDKMHLPRFVLINKMDRENADFNKVVTSIEEHSATRLVK
                     VQLPWGEKQDFKGIIDLLSMKAFKAEGKTPEDIPAELQSAAEEAHMTLVEAAAEGDDS
                     LLEKYLESGKLAPEEIIKGLTAIVRKGDFIPVLVSAGEKEIGVLPLLNVMIDIFPSPD
                     QVAGKVADGAAGEETLTAIDSEPLAAYIWKTTADPFVGKQTYFRVFSGMISADSRVWN
                     SSKETEERLGNLYAPRGKENMQVKVIHSGDIGVCPKLTVTATGNTLCDKGHPVKIRIP
                     EYPTSLYQVAIFPKTMADSTKISPTLTRLCEEDMTLSWHQEPATNQTILKGMGDQHID
                     VAIRRAESKFQVNLETSIPKVPYKETITKKATAMYRHKKQSGGSGQFGEVHLNVEPLE
                     GDAFEFVNAVFGGAISSSYMPAIEKGIRNVMKEGILAGYTVTGIKVEVFDGKEHPVDS
                     KPIAFEIAGREAFKLAFKDASPVLMEPIMLVKVIVPEEHMGDILGDMNTRRARVQGME
                     TEKGQSTVTANVPLSEMQRYTTDLRSITGGRGVFDMEFSHYEVVPAHITNVIVEKKAK
                     EDAEKEE"
     gene            complement(15253..17916)
                     /locus_tag="PLL88_06530"
     CDS             complement(15253..17916)
                     /locus_tag="PLL88_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ01261.1"
                     /translation="MDIIMTHEQTDFDGFASMLGAYLLNDRAHPLLPSTLNRNVQQFF
                     NTYHSELPFISPQALPKQNIRSVTLVDTQSMITLKGITKDTQVSVIDHHKKKEDFNPT
                     WNFTPVNCGACSTYFVEQLIEHNGNLSIIHATLLLLGIYEDTGSLTYANTTANDAQAV
                     AFLLSQGASLKIASEYLNLSLSNGQQKVFDALIKNHQIVERKKQRIMVSYADAEFLEE
                     EVSSIAHKLCDLYDPDALFIFVHTKEGVRFVARSTSDQINVAAIAARYEGGGHPRAAA
                     ALIRDAKKGLIALKQEFIDTLDEFVHPSITVKQIMSTNPLLLDPGITAQEALQFMQRF
                     GYEGFPVVENEKVIGLLTRRAVDRAIAHQMNLPAKSLMEAGNYYVYPETSLEELHHLM
                     ARANWGQVPVVHRQTGKIIGIVTRTDLLQAYSGFQVPLSNQLNLAAQLEENMDAGQLE
                     LMKIIASAAHELKMHVYLVGGVVRDILLNSPIYDLDFVVEGNAIELAHRLTELFGGKV
                     TSHRQFGTAKWFLENTKMPTAAAGAQTAELPKSVDLISARTEFYKNPSALPTVKLSSI
                     KLDLLRRDFTINTLAVQLDGKHYGVLSDYWGGLDDLKNGIIRVLHSLSFVDDPTRMLR
                     AVRFEQRFGFQIEERTLELLTEAKPLLKQVSGDRIRHEIDLILEERLSVDIFQRLGQL
                     GLLRSIHPDFSLSEKVTQLLQMALQAEPSQNWQLPIKIANYPVERIVQYCILFGSFPD
                     EVRDKISARLKLPALFQKSILQAHTLIERLPVLCNQKPSQIYSNLREIPHYVLYAVHF
                     FFHERDDILQILEQYLAVWSKIKPHSTGKSLQDRGISPGPVYKQIFQQLVDAWVDGKI
                     RSSAEEEQLLDSILTTLTRTGKVPDESSSQQ"
BASE COUNT         4684 a         4069 c         4355 g         4982 t
ORIGIN      
        1 aagagtttaa ccgaactgcg tgacaagatg atcgaaaaag gctttttacc cgcagaaaat
       61 gaaacggaat cggagagtta agaaatgcga catcaggttg ctggaaaaaa acttaatcga
      121 aattcaggga atcgcaaagc attgcgcagt accttgatta agcaattatt tacttataat
      181 aagatcaaaa caacccgtgc aaaagcacag gctgttcgta gtgaagccga aagattgatc
      241 acggtggcga agaacagcag caaaagcacc gatgctgaaa aggttcacgc acgccgcttg
      301 gttgcagcaa aattgaacaa cgctgctaca gtgaaacaat tgtttgacaa cattgccccg
      361 cgctttgaaa atcgaaatgg cggttacacc cgtgtaacca aattgggtcc gcgcaaaggc
      421 gatgatgccg aaatggtact tttagaactc gtcgaggacg agaagaagta atcattggat
      481 ttgtcccgta agggaatgga acattaccaa cttattatcg cttacgatgg tacggactat
      541 cgtggatttc agcgacaaaa caaaagcggt accatccagg gtgagcttga atctgcgctg
      601 cgtaagattg gctggcaggg agataccatt caagctgccg gaagaacgga cagaggagtg
      661 cacgctaccg gtcaagttgt tacctttttc ttcgattggg aacactcgct gaaagatttg
      721 caggcagcca taaattcaaa cctgccgccc gatattgcaa ttcagtccat acaactggtc
      781 aaagatggtt ttcatccccg ttacgatgcg gttagccggg aatatacgta tgcagtcact
      841 atctcggatg tccggcaacc cctgaaagag agaaccgtgt ggcggattga ccgggagttg
      901 aattggtcag caatgcaagc aacagccaaa ctgttcatcg gaaaacatga tttttcgcag
      961 ttcggcagag cgctgaaaga cgatggcagt acggttagag aggtgctggc ttccaattgg
     1021 gagcagaaaa cagccgccga ttatgaatat acgatttgtg cgaatgcgtt tctttaccac
     1081 atggttcgca ggattgtata tgtaatggtc aaggttgggc agggcaagat ggctgcacag
     1141 gatattcaaa gagcgttagg cggagaagcc gttaaaatag cccccggcat tgcccccgct
     1201 cgtgggttga cgctaacgaa agtccgcttt cgcgataata gcgaataatg acaaaagtga
     1261 aattattagg agaaagaaaa agtggaaaag acctttgtta taaaaggaaa accagaaact
     1321 gagtgggttt tgatcgacgc aagcggtcaa agcttaggac ggttggctgg tgatattgcc
     1381 aatatacttc tgggaaagaa taaggctacc tttactcccg gtgtgcttat gggagataat
     1441 gtggttgtga tcaatgcgga aaaaatcgtt attaacccga aacgcatgga agagaaggta
     1501 ttctacactc actcgaatta ccccggtgga ttaaaagctg tcaacatgcc tgatatgtta
     1561 aaaaaacatc cggagcgggt gttgagcaaa gcagtctggg ggatgatccc gcataataaa
     1621 tttggacgac aggttataaa acgtctgcat gtttacgcag gaaataagca tccgcacagt
     1681 gggcaagatc caaaaccagt gaagtgaagg agagataaga atgtcggctc agtactatga
     1741 aggaattgga cgtcgaaaaa ccgcaaccgc acgcgttcgc cttagtgcag gttcgggtaa
     1801 ttttatgatc aaccaaaaga atttagaaga atttttcacc cgttatggtg atacagaaac
     1861 catactggcg ccatttaaag cgaccggaga aaaccaggcg caatatgatg tgagcgtgtt
     1921 tatctctggt ggcggggtga acgggcagac cacagcggta caattgggtt tagctcgtgc
     1981 atttgcgaaa atgaaccctg attacgtatc ggtgttaaga aaaaaccgcc ttctcacccg
     2041 cgatccgcgc gaaaaggaac gcaagaaacc tggtctgaag cgcgcacgca aggcacctac
     2101 ttatacaaaa cgttaaaacc acttttttct tatctttcat aaaaatacta cccgccatca
     2161 gcaagtacaa ggcgggtagt ataattttcg aacaataaaa tcaaacaatt aaggaaagct
     2221 gatttgaata ctgatctgct caatacggtt ttggaatcga caaaacatca tgtaaccatc
     2281 atccctgcat cccttttaaa cgggagacat ttcccaccat ttttcagcaa tcagaatgca
     2341 ttgctcaccg gttttgtaaa aaaagaggat ctggttttcg cttttgaact tttagcgaaa
     2401 aactatcctg cagatcacgc gttgctgttt ttcaaacagg gggcggatag aaaggaaagt
     2461 tttattaaat ttgaaatgac ccttcaagac gtacggagca ggggacccca agacggggtt
     2521 attgccgtct tggtccccgc gaaaacagac aagaactcat ttctcgattt tttggaactt
     2581 attgctcact tgcgtgctcc tgagggatgt ccgtgggata gagagcaaac gcatcaaagc
     2641 ttgcgcccca atttgctgga agaaacgtat gaagtccttc aaaccctcga ttcacacgat
     2701 atgatcgggt tgcaggaaga gttgggtgat ttgcttttac aaatcgtgct gcatgcacaa
     2761 atagcgagtg aaaaccgtga ctttaccatc gaagacgtga ttacgggaat tgaacgaaag
     2821 ttgatcttcc gtcatccgca tatttttgga gataaagcgg tttctgatgc gaatgaggtt
     2881 atcaagaatt gggaggtcct caaagcacag gaaagaagag cgaataacaa agcccagggg
     2941 cttttgcgtt cagtgccaaa aggcatgtct gctctctcat tggcgcaagc gtatcaaaaa
     3001 agagcggcaa gagtggggtt tgattgggag tcgatcgaac cggtaaaaca aaaaattgat
     3061 gaagaattgc aagaagtgga aaaagctgat cgtgatgaag aacgtgccaa ggaattaggt
     3121 gatgtgttgt tcgcgatggt caatttaatc cgatggtatg ggcaagatgc agaatcagtt
     3181 ttgcgcgaag cagcggaaaa attcgcgagc cgttttgaat acattgaaga ttgcgtacaa
     3241 aaaagcggga agacctttgc agatttttcc cttgctgagt tggacgcttt ttgggaagat
     3301 gcgaaaaaac gataatcttc tttatgaaag aggggcgaac ttcgccgtaa aatgccgcaa
     3361 taactttggc tcgtaggtaa ttcgataccc tcgaatactc tgacggtgtt gaccgacctt
     3421 ctttaatatt tccaccacat agtccaagtg actttgggta tatacacggc ggggaatcgc
     3481 aagccgcacc aattccagtt tcgggaaaat cacttcacct gtctgcggat cagggtgggc
     3541 aaacatgaca gatccaatct cgacaccacg aatccctcct tccaggtaaa aggcggcagc
     3601 caacgcctga ccagggaatt cagcatgggg gatgtgtggc aagaatgaac ctgcatcaac
     3661 gtagatggca tgcccgccgg gcggttcaat gatcggaatg ccgttttcga ttaatcggct
     3721 gcctaaatat gctgtctgcc caatgcgata agccagataa gcttcgtcca gaccttcgcg
     3781 caatccaacc gccatcgcat ctagatcgcg acctgccaga ccgccataag tgatgaagcc
     3841 ttcacgcaag atcaattcat tgctgatctt ttggaaaaga tcggcatcgc gaactgccag
     3901 gaaaccgcca atattgacaa tggcgtcttt ctttgcgctc ataaaggcac catcggctgt
     3961 tgcaaagatc tcattggcga tctccatcag tgatttttct tcataaccgg gttcgcgttt
     4021 tttgatgaaa taacagttct cggcgtaccg gcagcaatcg atgaaaaagg gaataccgta
     4081 ttgatgatag agttggctgg tctctcggat attctttaat gataccggtt gccctccgcc
     4141 ggcattattc gtaattgtga tgcacccgaa ggggattctt tggggtccaa ctttgcggat
     4201 caattcttcc aatttgacga tgtccatatt ccctttaaat ggattggtgg attggacgat
     4261 aaaagcatca ccgattacgc aatttgttgg atctccaccc cgagcgcgta tgtttgcgtc
     4321 ggtggtatca aagtgcatgt tcgatgggat gattgctccg gcttttacca gtatggtcga
     4381 caagatgttc tcggctgccc gcccttgatg ggttggaacg aaataatcaa aaccgaatat
     4441 ctcttgcata gtttgtttga ggtgatagta agacttggca cctgcataag attcgtcacc
     4501 ctgcatgatg gctgcccatt gattctggct cattgcgccg gtgcctgaat cggtaagcag
     4561 atcgatatag acatcttcag ctttcaaggt gaaaaggttg taccctgctt cctgcatggc
     4621 ttttgttcgt tgttcagagg agatcaagcg tattggttca acggatttga ttctaaaagg
     4681 ctctggtgga tgtggctgca tttctattct cctattacaa taattctatg gtttagtgta
     4741 tcaacatttt tgctagaccg cgtgtgtaat aaatactctt tctttttatg gtaaataatg
     4801 aaaattctaa aacacaatac ctttttacga tttatatcag gggtgatctt ttttgtctcc
     4861 agtatgtgtc tgatcgtcac cagttttgaa ggacctgtcg ttgagaaaat gtatcaattc
     4921 cccgagagtt ttattgaaaa tgaggttgac cctgcctggc aggaatcggt taccaatgcg
     4981 cttgcctcct atcaaataaa tcttcgtcac cctgaatggg tgtggtatgg ggaatctttt
     5041 tttatccagg tttcgctcat cgagcgcgca ggattaccca cttcagaaaa caatgctctt
     5101 ccatcttttg ttttggaagc atatttggaa atgaacaatg tgggtaccac cccaggagaa
     5161 catatcctgc tgccaatcca attaccgcaa actggcttgc ttcaatggga ggtagatcct
     5221 caggctgaag aaattgagca aagtagagta tggctttcta tcttgcctca agaagggggc
     5281 gactcttcca taccggtact tgttttaccg cttgaaataa aaattcgtcc ccttttgggg
     5341 ttaaagcttg gaggttggca gatgatctgg attggcttgg gggtgatcag catcggcttt
     5401 tatctttcag accacctcaa gcgcaacaga cgtgtttgaa tgttataatc tgcctttgtg
     5461 aaagaccttg acgaggatat ccctgctatt aacttaacca aaaagtcatg cgaaaacatt
     5521 acctgatatc tgtcgtgatt tctgttgcta tcactgcgac ggtcattgga attggctatc
     5581 tgattgacca atggcaaggg agcacgccaa cctatatgct gattggattc ataatttctg
     5641 gtccactttc ggtatgggca aattacagtt tgatcaagaa aaaattcaca agttcaccag
     5701 aaacaatgaa aggacctgac caaaaatcaa agccatgaaa tcatccgtta ctgttcgcga
     5761 gattttttcc tcttgtccgg gaatactggc agctccgact accttgccac aattaccggt
     5821 tgccggtgtg gagtttgatt cgcgggaggt gacaaaaggg agtgtatttg tggctatcga
     5881 aggggaatat caggatgggc acgcatacat tcgatcagcg ctcgatcggg gagccgtgat
     5941 gatagcgggc acgcaacaga aatggttgga gatgctacct gaccagtatg tatgggtgga
     6001 tgatggacgg aaagcattag cctatctggc tgctgcatat tacggtttcc cggctcgcag
     6061 aatgacagtg cttggggtga ccggcaccga tggaaaaacc accaccacca acctgatcta
     6121 tcaaatcctg aagcaggctg gattcaagac gggaatgatc tcaaccgtga atgccgttat
     6181 tggagatgaa accgttgaca ctggttttca tgtaaccaca cccgaagctc ctatggttca
     6241 gaagctcctg gctcgtatgg tgaattgtgg cttaacgcac gtggtgctcg aaaccacctc
     6301 gcacgggtta gcccaacaac gagtggctgc ctgcgaatat gatattggtg tgttcaccaa
     6361 tattacacac gaacatcttg attatcacgg tacgtatgag aattatgtgc ttaccaaagc
     6421 ccgcctgatc tcattcctgg cggaaacgaa gtccaaagag cttggagcca tacggttggc
     6481 ggtgttaaat aaggatgatc agtcgtaccc ctttttgcaa aagattttga agggaattgc
     6541 cggcgatgca gttcatagta tttcgtatag ccgagaaaat agtgcggata tatgcctctt
     6601 aaatgaagcg catttcccca atgggttatc gattgagctt gcgatcaggg atcagaaggt
     6661 ctccatacac agtaatctca ttggctccta taatgtttcg aatatcatgg cagccgtggg
     6721 cgcaacttcg atcggtctgg atattgcgcc cgaggtgatc agtgctggca tagctgcttt
     6781 gccagacatt ccgggaagga tggaaaggat tgacctgggg cagaatttta cagccatggt
     6841 ggattttgcg cacacaccca atgcaataaa ggtaaccctt gaaacagcga gaaagattac
     6901 aaaaggacgg attattgcga tattcggatc ggcaggtctg cgtgatcgtg aaaaaagaaa
     6961 attgatggct gtggaaggtg taaagcaggc ggatttatgc atcttcaccg cagaagaccc
     7021 gcgcacggaa aagctggagg atattcttgc agaaatgaaa ctggctgctg aaaaggtggg
     7081 tggagcgctg ggaaaagact tttttatcga acctgatcgg gggaatgcta tccggctggg
     7141 gctacaactg gcagagacag atgacctggt ggtcgcatgc gggaaggggc atgaacaatc
     7201 catgtgtttt gaaaagacag agtatttgtg ggatgaccgt atcgccatgc gggcagccct
     7261 ggcggaactg ctcaaaatag aaggtccccc aatgccttat ctgccaaccg caaaataaaa
     7321 aaagagctgg atcaaatcca gctcttttca ctcatctttt cttaatctta tcttttagtg
     7381 cgttttccgc tatggctgcc actttttctc tcaccgcttt tattgttatg gttgtcatgc
     7441 tcgcgcgctt cttccacgct ccaaccttca agtacagcca catgagaaag gcggatcttg
     7501 ccggagcggg ggtcgatctc ggtgatcatg acggagagtt catcgccttc aacgaccaca
     7561 tcctgaacgg acttcacacg gtctacactg agctgtgaaa tatggaccag cccttctgtt
     7621 cctggcaaga tctcgacgaa tgcaccgaaa tcgacgacct ttgtgacttt gccggtgtaa
     7681 atatgaccaa tagtaggcac ttcgatcatc ttctcgatct gagaacgtgc gttcatttca
     7741 tcttgtttat ctacggcagc aatgaacact gtgccatctt ccgcgatatc gatctgggtt
     7801 ctggtctctt cctgtaaatt gcggatgttc ttgccgccag gaccgatcaa tgcaccgatc
     7861 ttatcaaccg gaatcttgac tgtggtaatg cgtggcacaa actctttcaa ctcaggacgc
     7921 ggttttgcga tcacttcagt gatcttatca agaatagcca atcgagcttc gcgtgcctgg
     7981 gcaagcgctt cagacatgat ctcaggggtg atgcccttga tcttgatgtc catctgcaat
     8041 gcggtgatgc ccgcggtggt accagcgact ttgaaatcca tatcacccag gtgatcttcc
     8101 agaccttgaa tgtcggttaa aacggcgtac tgctcatttt ctttgatcaa gcccatagcg
     8161 atgccggcga ccggagcttt gatcggcaca ccggtgtcca tcaatgccag ggtggaaccg
     8221 cacacggatg ccatggaggt ggaaccgttg gaggaaaaaa cctcggagac aagccgcatg
     8281 gtatagggaa attcggattg agcaggaagt accggtacca gtgctctttc agccagcgcg
     8341 ccgtgtccga tttctcggcg agaacgatgc gggcgctgca cttctccggt agagaagggc
     8401 gggaaattgt aatgatggat ataacgcttt gactcagttg gggtcagatc atcgatctcc
     8461 tgggcttctt tgggtgttcc gagggtagcc agggtcatta cctgtgtttc cccacgcgta
     8521 aacagacctg aaccgtgagc gcgtgggctg acattcactt cacaccagat gggtcggatc
     8581 tgtttcggat ctcttccatc cgggcgaatt cctttggtca aaatgcgcgt tcggacgacc
     8641 ttttgatagg catcatgaaa cgcttctttg acgtcatgca gaagttttgc atggtcttca
     8701 tcggtttcgt ttgcatattc ctccagcaat tcttcttgga tggcatcgat ggcagcgtcc
     8761 agttcagctt tgctgtgtgg tttgtcaagc gcatcgttga tcttggtggc gcccttctca
     8821 tagacctgtt cgatcaaagc ctggtcaatg ttggtcagtg gcacatcccg tttcgttttt
     8881 ccaatttccg cagccatttt ttcttgaact gcaatgatcg attgaatgga ggtatgacca
     8941 aaactgatgg cggcgatcat gtcgttttcc ttgacctcat ttgccccgca ttccaccatt
     9001 aagatggcat cttttgttcc tgcaatacgc aggtcaagat ctgatatctg catctgttca
     9061 aaagttgggt cgacaacgaa ctttccatca atgtgcccaa cgcgcactgc accaactggt
     9121 ccagcccagg gaacgtcaga aatgaccagg gcggctgatg cggcgttgat agccatgata
     9181 tccagtgggt ttacaccatc tgaagagatc ggaaacatca tgatctgcac ttcattgcgc
     9241 atgttcttgt caaaaagagg gcgcaggggg cgatcagtca ggcgagcgac cagtactgca
     9301 tcatcgcctg gtcgtccctc acggcggaag aacccaccgg ggatgcgacc acccgcatac
     9361 attttctctt catattccac gctaagtggg aagaaatcgg tcccttcaat aactgtgttc
     9421 gacattgtgg cggaggcgaa aagaacagaa ttgccaaccc tcagtgttac tgcgccgcct
     9481 gcctgccctg ccaattttcc ggtttcgaac gtaaattcgt ggtcaccgat agtgccactg
     9541 taactaaaag ctttttgttg catttttact ctctttcccc cgcatagtta ggaactgagg
     9601 gttaaagaaa ataccttttc gtcaactctc aatgcccaac tcgcgggatt ttcccataaa
     9661 aagagtagat ggcttttctt caaccatcta ctccagatgg gttttttcct actttgttcg
     9721 tatatttagc ttttcggtta tcgcaacgta ttttttgtaa tctttcttct ttaagtatgt
     9781 aagaaaactt ctccgttgac ctacaagctt gagcaaccct cgccgggaag actcgtcatg
     9841 tttatgtatt tttaagtgtt ccgtaagttg gttgatacgg ttcgtgagga gggcaacttg
     9901 cacctctgtc gagccggtat cattttcatg ttgcttgtaa ctctcaataa ccttttgttt
     9961 ttcttctttt gtcattgtca tgacattgct atctcctttt tctcagcaag tctcctcttg
    10021 ggcagtgctt atctgaccgc cgaagggact agtcatgtgc attattatac cagatagtca
    10081 atgctgaaag agtttgtgcc ggttctcttc tataagactt ctataagata tttttgattt
    10141 tcaggggtga aaaaatggat gatttttcgg acgtatttcc ttgctttcgt actgttagcg
    10201 tgcgtgcata tttcatacaa gaaagcgatc gtctcaattt ctgactttct aatcaacgct
    10261 gtcatgaccg gatttaactg cccataggtt tggagagaag cggcttcaat gaatggagcc
    10321 aatatattaa agacccaggg aagatcagaa aaatgtttct gctctataac ccttgccagg
    10381 atgtgaaaag ccaattttct catttcaggg tcatccatat gcatcaggtg ctcaatgaag
    10441 gtttgtatac tggttgaatt ttctttgtgt gagaagggaa gctgtgcaag gactacactg
    10501 tgaagcaact cctcatcggt agagctgacc cattgcagaa gccttttact gatctcttct
    10561 cttttttcta caggcaggca taataataag ttaaccgcaa gtattttata ttccaactct
    10621 tctcgatccc ataaggtatc taatagatca atcgcattct cgaggaaatc atgggtgagc
    10681 gccggaaatg tctcgatgat tttttccagt accaggtcgg gcacatagag gcgacgaaga
    10741 agggtttgga agggtgtggt ttctcctaac tttgtcttct cggaggaata catactcaac
    10801 agatcttcca gtttttgcag atagatgcgc gcgtcacttc tgaaaagctg aagggattga
    10861 agtcggctat ttaagacggt caaatcaaca gcgggcattg gttccgatca atatgccttg
    10921 gcgaaatagg ctttcgttgc agtctctttg ccgcaaacca cgcatgtccc ttgtgtgtga
    10981 tcttgatgga aggggatgca gcgggtagtc gcctttgtat cttccttaat tttgacttcg
    11041 caatcttttc cttcgcacca atagctgaaa gcccagccat tttcaacgct cttcttcaga
    11101 tcatcgtatg tcgctgcttc gtggatgtgg gaattgcgga aggatgtggc tcgttgcaac
    11161 aaggagctat ggatcttatc cagcaagtac gctgttctgg gatggagtgc gtcaagatcc
    11221 ataacctctt tcccggcttt tcccggaatg tcacgccggg cgatcaccag tttgttgttg
    11281 gcaacatcgc gaggtcctat ttcgatacga agtggcaccc cccgcatttc ccaatcattg
    11341 aacttgaaac cgggggtttg ctcgaaacga tcatccaact taacccgaaa atcagatagt
    11401 tcagtcatga tctcttgcgc atattgcatg accgcttcgc gttcttcgag gttgtggaag
    11461 atgggaacga tcacgatctg gatgggagcc aacctgggcg gtagcatgag accttgatca
    11521 tcgccatgtg tcatgataat agcaccaata aagcgtgtag aaagacccca agaggttgtc
    11581 cagcaatatt ggagttgatt ttgatcatca aggtattgga tatcgaatgc cctggcaaaa
    11641 ttttgcccaa gattatgcga agtgcctgat tgcagcgctt tggtatcccc catcatggct
    11701 tcaatcgtat aggaatgaag tgccccagca aatgtctccg tttcgctctt catgccgcat
    11761 atcaccggaa tcgcggcttc gttgacggca aaatcttcat agacatgcaa catgcgcagg
    11821 gtttcctctt ccgcttcctg tgctgtggcg tgtgcggtat gcccttcttg ccagtaaaac
    11881 tctaaagtgc gcaggaagag ccgcgtgcgc atttcccaac gaaccacatt agcccattgg
    11941 ttgatgagca caggcaggtc tcgatacgat ttgatccatt ttgcatacat gtgtccaatg
    12001 atcgtttcag aggttgggcg taccaccaga ggctcttcca gatcctgacc gccaccatgg
    12061 gtgacgaccg caagttctgg cgagaaccct tcaacatgtt ccttttcttt ttcaagaaat
    12121 gacatcggta taaagagggg aaaagcggcg ttttgatgcc cggtgtcctt gaagcgttta
    12181 tcaagcgctt tttgaatgtt ttcccataat gcccagccat acggtttgat caccatacaa
    12241 ccacgaacag gtgcataatc agccatttct gctttttgga tgatttcatt gtaccaatct
    12301 gagaaattat cgtctctagg ggttagtttt tcgttcgcca tgcttcctca ttttctctac
    12361 gtaaaaatta tccagttgtg caactgcctc ttcgatattg aggcagaagc ggatgtattc
    12421 tctgaccgaa gttggaacaa agcagggcat ttcatcaaaa aacgttgtca tcactctttt
    12481 ccaaccttcg ccgaccaaca gcagataggg aacggggatg gattctaaga tgatcatatt
    12541 ccaggttaaa gcgatttcag ccaatgtgcc aacgccgccc ggtaatgcaa tagctgcatc
    12601 gcaattcctg accaagccac tgatcctgcc gaacagatcg gtatggtgca ttacttcatg
    12661 cacccaaggg tttggcttat cgttacgcca ccgttcaata tcatcacagg ttactccgat
    12721 cacatgccca ccggcttccg cagttccttt ggaaaccgct tccatggttc ctacataccc
    12781 gccgttcaag acaatgtagc cacgctcagc gaggcatttg cccagtcgat atgcttccag
    12841 atattctgtg tccccaggtt tgggatgtgc gctgccaaaa actgatactt tcattaaaaa
    12901 ttaatcctgc ctgcttgata agtgcgctct cgtaatcaca tgacactaat tgaattattt
    12961 tacttgattt ctatttttta aataaaagag gagttgcctt caactcctct ttattgccat
    13021 tgcctgagcg ttattcttct ttttcagcgt cttctttggc tttcttctcg acgatcacat
    13081 tggtgatgtg cgccggtaca acttcataat gggagaattc catgtcaaaa acaccgcgtc
    13141 caccagtaat ggatcgcaga tcagtggtat aacgctgcat ttcagataga ggaacattgg
    13201 ctgtgacagt ggattgtcct ttttcggttt ccatgccctg cactcgtgcc cgacgggtgt
    13261 tcatatcacc gagaatatcg cccatatgct cttctggaac aatcacttta acaagcatga
    13321 tcggttccat gagaacgggg ctggcatctt tgaacgctag tttgaatgct tcacgaccgg
    13381 cgatttcaaa ggcgatcggt ttggaatcaa ccggatgttc tttcccatca aaaacttcga
    13441 ctttgatgcc tgtaaccgta taaccagcca gaatgccttc tttcatcaca ttgcggatgc
    13501 ctttttcgat agcaggcatg tagctggaag agatcgcacc accgaataca gcattgacga
    13561 attcaaaagc atcgccttct aatggctcaa cgttcaggtg aacttcgccg aactgaccgg
    13621 aaccgcctga ctgttttttg tggcgataca tggcagtggc tttcttggtg atagtttcct
    13681 tgtagggcac cttaggaatg gacgtttcca ggtttacctg gaactttgat tcagcgcggc
    13741 gaattgccac atcaatatgc tggtcaccca tacctttcag aatggtctga ttggttgcag
    13801 gttcttggtg ccatgacagg gtcatatcct cttcgcatag gcgagtgagg gtagggctga
    13861 tcttggtcga atcagccata gttttcggga aaattgctac ctgatataaa gaggtgggat
    13921 attcaggaat gcgtatcttt accggatgcc ccttatcgca gagggtattt ccggttgcag
    13981 taacggtcaa ttttggacag acaccaatat cgccactatg aatgaccttg acctgcatgt
    14041 tctctttccc acgcggcgca tataaatttc ctaaacgttc ttcggtttct tttgatgagt
    14101 tccaaactcg tgaatcggca ctgatcatgc ctgaaaatac tcgaaaatag gtttgcttgc
    14161 caacaaaggg atctgcagta gttttccaga tataggctgc cagcggttca ctgtcgatgg
    14221 ctgtgagggt ttcttctcct gctgcgccat ctgccacctt gcctgccacc tggtcgggag
    14281 aggggaagat gtcaatcatg acattgagga gagggagaac gccgatctct ttctcgcctg
    14341 cgcttaccaa tactggaatg aaatcccctt tacgtacaat agccgtaagt cccttgatga
    14401 tctcttcggg ggcgagtttt ccagattcga gatatttctc caagagagag tcatctcctt
    14461 cagcggctgc ttcaaccagg gtcatgtggg cttcttcggc tgcgctttgg agttcagcag
    14521 ggatgtcttc gggtgtcttt ccttccgctt tgaaagcttt catggaaagt agatcaataa
    14581 tgcccttaaa atcctgcttt tctccccacg gtaattggac tttgaccaaa cgggtcgccg
    14641 aatgttcttc gatggacgtg accactttat tgaaatcggc attttcacga tccattttat
    14701 tgatgagaac aaagcggggc aggtgcattt tatcgcaatt cttccaggca acctcagtgc
    14761 caacttcgta tcccgacact gcgtccacga cgatcactgc gccttccacc acacttaatg
    14821 cagagatcat ctcgccgaca aaatcggtgt agccgggtgt atcgataaaa ttgattttgg
    14881 tatctttgaa ttcgacggga atgacggagg aatataagga tatgctgcgt cgaacttctt
    14941 cttcatcaaa atcactgacc gtcgttcctt cattaatact acccatgcgg gtggtggcac
    15001 ctgtagtgtg aagaaatgct tcaacgagaa ctgtctttcc ggcactactg tgtgaaacaa
    15061 gagcgatatt tcttatgttg cttgtgctgt attctttcat taatattacc ctttaaaaga
    15121 gatatgataa caacccgatt attttaaggt gaatcgccct aaatgtcaaa gatgtgctgt
    15181 gcatgagcga agagataaaa aagtgcgcaa ttccaggcag atattatggc gcagagaccc
    15241 ttgaaccatt tcttattgtt gagaagatga ttcatctggt actttgcctg ttcttgtaag
    15301 cgtagtcagg atggaatcaa gcaattgttc ttcttctgcg gatgatcgaa tttttccatc
    15361 cacccaggca tctaccagtt gttggaatat ctgtttgtac acaggaccgg gggagatacc
    15421 acgatcttgc agggactttc cggtactatg cggctttatt ttgctccata cagctaaata
    15481 ttgttcgaga atttggagga tatcatcacg ctcatgaaaa aagaaatgga cagcatagag
    15541 cacgtaatgc gggatttctc gtagattgga ataaatctgg ctgggtttct gattgcagag
    15601 cactggtaat cgctcgatca gtgtatgggc ttgtaggatg cttttttgaa acagagctgg
    15661 caattttaag cgagcgctta tcttgtcgcg tacctcatcc ggaaacgatc caaaaagaat
    15721 acaatattgc acgatgcgct caacggggta attggctatt tttatgggaa gttgccaatt
    15781 ctgcgatggt tctgcctgca atgccatttg aagcaattgc gtgaccttct ctgaaagaga
    15841 gaaatcaggg tgaatggatc gaagcaatcc caattgaccc agacgttgaa agatatccac
    15901 cgacaatctc tcctctaaaa tcagatcaat ttcgtggcgt attcgatccc ctgatacctg
    15961 ttttaaaagt ggtttggctt ctgtcagtaa ttccaatgtg cgttcttcga tctgaaatcc
    16021 aaagcgctgt tcgaagcgca cagcacgtag catgcgggtg gggtcatcga caaaagaaag
    16081 tgagtggagc acacgtatga ttccattttt cagatcatcc aatccacccc aataatctga
    16141 tagcaccccg taatgtttac cgtctaattg gacggcaagc gtgttgatcg taaaatcgcg
    16201 gcgcagcaga tcgagcttga tactgctcag tttgactgtt ggcagcgcac ttggattttt
    16261 ataaaattcg gtgcgtgcac tgatcaaatc tacagacttc ggcaattccg ctgtttgtgc
    16321 ccccgcggct gctgtgggca tcttggtgtt ctcaaggaac cattttgcgg tgccaaattg
    16381 gcggtggcta gtcactttgc caccgaaaag ttctgtcagc cggtgtgcca gctcgatggc
    16441 gtttccttct acaacaaaat caaggtcgta gatgggcgaa tttagcaaga tatcgcgcac
    16501 aaccccgccc accaggtaaa cgtgcatttt taactcatgc gctgcgctgg caatgatctt
    16561 cataagttcc aattgacctg catccatgtt ctcttccaat tgagccgcaa ggttcagttg
    16621 attgctcaaa ggaacttgaa atccagaata cgcttgcagc aggtcggttc gtgtaacgat
    16681 gccaatgatc ttgccagttt gccgatggac cacaggcacc tgcccccaat ttgctcttgc
    16741 cattaaatga tgaagttctt ctagcgacgt ttcgggataa acgtaataat ttccagcttc
    16801 catgagactt ttggcgggaa ggttcatttg atgcgcgatc gcacgatcta cggcacgccg
    16861 ggtcaacaat ccgatcactt tctcattttc gaccacgggg aaaccttcat aaccaaagcg
    16921 ttgcatgaat tggagcgctt cttgtgctgt gatgcctgga tctaacagga gcggatttgt
    16981 tgacatgatc tgtttgacgg tgatggaagg atgaacaaat tcatcgagtg tgtcgatgaa
    17041 ctcttgtttt aatgctatga ggcctttctt tgcgtctcgg atcaatgcgg ctgctgcgcg
    17101 tggatgtccc cctccttcat atcttgctgc aatggctgcg acattgattt gatcgctggt
    17161 cgatcgagct acaaaacgga ccccttcttt ggtgtgcaca aaaatgaaga gagcatccgg
    17221 gtcgtagaga tcacatagtt tatgggcgat gctagaaacc tcctcttcca aaaattctgc
    17281 gtcggcatac gaaaccatga tgcgctgctt tttccgttcc actatttgat ggttcttgat
    17341 cagcgcatcg aaaacctttt gctgcccgtt ggatagggaa aggttcaagt attcgctggc
    17401 tatcttcaaa ctggcgccct gcgagagcaa aaaagccaca gcctgcgcgt cattggcggt
    17461 ggtgttggcg taagtcagag atcctgtatc ttcataaatc cccagcagca gcaatgtggc
    17521 atggataatt gatagattcc cattgtgttc tattagctgc tcgacaaaat aagtggaaca
    17581 tgcgccacaa tttactggag tgaaattcca ggttggattg aaatcttctt ttttcttatg
    17641 gtggtcaatg acactgacct gggtatcttt ggtgatccct tttaaagtga tcattgattg
    17701 ggtgtcaacc aatgtgaccg agcggatgtt ttgcttgggc aatgcttgag gagatataaa
    17761 aggcaattcg gaatgatacg tgttgaaaaa ttgctggacg tttcgattca atgtggatgg
    17821 caaaaggggg tgggcacgat cattgagcag ataggcaccc agcatggatg caaacccatc
    17881 gaaatctgtc tgttcgtgcg tcatgatgat atccatactg ttattctaat ctttcttttc
    17941 taaaaaagca ttgcgcttct tttacgctca cactacaata gctatgagaa aaatttgagg
    18001 tttacatggc agagacaaaa aagattcttc tggttaccgc tcatcctgac gatgagacgt
    18061 ttggtatggg cggaacgatc gcaaaatatc
//
LOCUS       DAOSDS010000044        17316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1341_length_17316_cov_5.886623, whole genome shotgun sequence.
ACCESSION   DAOSDS010000044 DAOSDS010000000
VERSION     DAOSDS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 17316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17316
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1341_length_17316_cov_5.886623"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLL88_06535"
     CDS             <1..387
                     /locus_tag="PLL88_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HOJ01262.1"
                     /translation="DVLRMRNELGISMREMNLTPHKLAALVNLTDAGKINITTAKNLL
                     SKIQESGKNPQVLVEELGLAVVSDDAAIRAVCEKVVAQNPNEVQSYKNGKESLIGWFV
                     GQVMREMRGKADPRLTQQILKEILEK"
     gene            complement(424..894)
                     /gene="bfr"
                     /locus_tag="PLL88_06540"
     CDS             complement(424..894)
                     /gene="bfr"
                     /locus_tag="PLL88_06540"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HOJ01263.1"
                     /translation="MKGNEKVIETLNHLLADELTAINQYMVHSEMAADWGYEALHEIS
                     EKRAIDEMKHAETLIGRILFLEGVPVVEKLNPIHIGKEVKGQIDNDRIAEQDAIKAYN
                     AAIKLTGEFDDFGTREILEKILKDEEAHIDTLESQLSQIEQMGIQVFLAEQLED"
     gene            1070..2140
                     /locus_tag="PLL88_06545"
     CDS             1070..2140
                     /locus_tag="PLL88_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ01264.1"
                     /translation="MRTFASGSKQVKRVTIKEVAAKADVSYQTVSKVINHQVEVLPET
                     AERIWAAVEELGYRPNYTARSLRSKRSYTIGYTWSPTPHGLTNPILDQFLQSMFHTAE
                     QAGYYLLCFPYHQENERQLNVYSELIDTGRVDGFILSSIEYEDPRIAFLFDRNFPFVG
                     FGRSDDDMVFPYIDVDGGMGIYQSVLHLAEGGRCRIAALAWPEDSRVGNNRMAGYFHA
                     MEECGLPIDISLIARGAGNYEFGYQACDELMQLPLSMRPDAIVALNDYMAMGAMAAIQ
                     KQGLIPGEEVAVTGFDDNPIAQYLRPSLTTVQQPIWDIGQKVIEMLVYQLEEGKTPDP
                     VCSLIPPRLIVRQSSQPKGEKR"
     gene            2392..3405
                     /locus_tag="PLL88_06550"
     CDS             2392..3405
                     /locus_tag="PLL88_06550"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ01265.1"
                     /translation="MDLKQIAKQIGVSTATVSRVLNDQPGVSAEVREKVLKAAAEANY
                     HPNHIARSLATSRSQTIGFVVHKEKYAFSDDPFYPLILSGAEEYLSQHGYNILITLLD
                     EKEKQPQNLPIIHQRLVDGLILAGPFIQASSVMSIASTGIPFVLVDNSLSQYPVNCVL
                     TDDEGGSYSGVRHLIEEHQQKKIAFLSGPDEWISNRERLRGYERAIQEFDLEPRVIHA
                     KETTIASGEEMMRQALERWPDLTAVYGVNDSVAIGAIRAAAKRGRSSPRDVAVMGFDD
                     ISWAAANQPSLSTVKIFTQRIGYLAAQCLLNATQQPGLPPTKTLVTTELVIRESCGCQ
                     ENT"
     gene            3489..4358
                     /locus_tag="PLL88_06555"
     CDS             3489..4358
                     /locus_tag="PLL88_06555"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOJ01266.1"
                     /translation="MSVNSVFNAGIVKWNDQYVLLTRVEDLSGSSFLWAARSQDGLNF
                     TPDPRPALLPAKEGVYAPVEDCSIEDPRITPLEGSFYVTYVGYSCHDCVSILARTDDF
                     LQFERVSILGLPENKDVVLFPEKIGGRYAKLDRPMTQTSQRGDMWISFSPDLLYWGDP
                     RPVMQIRQRKWDCLKIGAGAPPIKTSEGWLVIYHGVRPTSAGSIYRLGAALLDLDQPW
                     RVVGRSEQAILSPIAAEDYLGNVGNVVFTCGAILEENGELKVYYGAADQVLCLASAPV
                     SDVIALCLEGKTE"
     gene            4369..5250
                     /locus_tag="PLL88_06560"
     CDS             4369..5250
                     /locus_tag="PLL88_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HOJ01267.1"
                     /translation="MVLKRYAATPILEPDPQHAWEALNVFNCAVVVANDLFHMLYRAQ
                     GVARVSSIGYAVSSDGVHFNRLQQPVLAPQDEWDERGVEDPRVTFLPDERRYIMAYTA
                     YSSTGSTPFFAESRNLISWQRIAPLVVREDNKDHVLFPCKIKGRYVTFTRRIPNIGLA
                     FSRDLKSWEDFQPLITPRPGKWDSKRVGAGGPPIQTEQGWLCIYHGYNEENSYRLGSF
                     LLDLDDPSRVLARAEGFLIEPQEPWELRGDVPNVIFSTANPVVNETVYVYYGGADRVI
                     GLATCALKDLLDFTLHG"
     gene            5283..6938
                     /locus_tag="PLL88_06565"
     CDS             5283..6938
                     /locus_tag="PLL88_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ01268.1"
                     /translation="MMRSTAANKDGNWWRDGVIYQIYPRSFLDADGDGVGDLQGVIEK
                     LDYLNDGTERSLGIDAIWLNPIFPSPMADFGYDVSDYCDIHPLFGDLSTFDRLITESH
                     RRAIKVILDFVPNHTSDAHPWFLESRSSRENPKRDWYIWRAAKADGSPPNNWGSAFGG
                     PAWEWDETTGQYYLHSFLKEQPDLNWRNPQVKKVMLDVLRFWLERGVDGFRMDVIGML
                     LKDAELRDNPLSEEASSSDSASLYARQQHIYDQDQDEVHEIIREMRKVLDEYPERCGI
                     GEVWYELPRYVQYYGSDGDELHLPFNFRLMKIPWSAQAVKQSVDEMEGILPDFAQPNY
                     VLNNHDSPRLISKIGAEQARVAAMLLLTLRGTPTLYFGEELGMENGVIPPDRMQDPQG
                     FRLGAKYSRDLSRTPMQWTAESNAGFTSGQPWLPLTPSYQNRNVQAEDNDPTSMLTLY
                     RKLIHLRKKRTALHGCYVPVSVESVDAFVYERCQENERYLIALNFSGEKITMRIPDFS
                     EGKVLLSTYFDQEGCTVTNDLELRENEGVIIAVKNTVARPQDE"
     gene            6942..9827
                     /gene="pgmB"
                     /locus_tag="PLL88_06570"
     CDS             6942..9827
                     /gene="pgmB"
                     /locus_tag="PLL88_06570"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HOJ01269.1"
                     /translation="MNNWIISEDSFAVSNLNSQETVFTIGNGYLGTRGTFEEGFSGES
                     AATLLHGVFDDTPIGFTELANTPNWMDLRVYVNEQVFRLDEGEVVSYQRTLDLHNATL
                     KREITWLSPVGNTLRFEFERFSSLADEHVLAVRCRITSVDYNGPLEIRARVAGHVENS
                     GWTHWNWLGQGMTENGMAYLCLQTRKTEIELCEAFTLKLVKDTPQYEGYWDSLNAPEK
                     VLKTSLQAGRSICVEKLVAVYTSRDTSDPQKAAIEKLHSAEGKGYEKLWEEHCAQWEE
                     EWKSSNVLIEGDDKADRALRYGLFQLLIAAPRHDDRVSIAAKSLSGFGYRGHTFWDTE
                     IFILPFFTYTRPEIASNLLRYRYHTLEGARKKARAKGFEGACYAWESAATGEETTPRW
                     ALLPDGGLVHIWCGDIELHITVDVVFAIYQYWQATGDDGFMLDYGAEIILDTAVFWAS
                     RVEWNEEQRHYEITDVIGPDENHEHVNNNAYTNNLVRWNLQKGIEVLRWIQNAAPEKA
                     AELEKRLGITSAHLSAWKSIIEKIYSGFDQQTGLFEQFTGYYQLKPLDLTSLEPRTRS
                     VQSILGIEETQKVQVIKQPDVLMMLYLLNHEYDESLLKANWDYYAPQTDLTYGSSLGP
                     AIQSIMATRVGDIEEAYRLFMLAAGTDLEDKRGNTAEGIHAATHGGLWQTCVFGFGGV
                     RFTADGPVASPHLPTGWKRLQFEIQYRGVGYTFDLHAGSEQPVLPGKKVPSDEKQKSA
                     MGISGAIFDLDGVITDTSEFHYLAWKRLAEEEGIPFDRSQNDALRGVSRRESLQKILG
                     DRTVSEEQMQAMMERKNGYYLDYLNSLTEADLLPGVLDFMKDAKRQEVKLAVGSASKN
                     TQLVLEKLGIRDLFDAIADGFSVVHVKPAPDLFLHAADQLQVEPEHCIVFEDAEAGIK
                     AALDGNFWAVGVGPQERLHAAHLVISDFKGLNWGTFMDQMEKTQKNI"
     gene            9957..11273
                     /locus_tag="PLL88_06575"
     CDS             9957..11273
                     /locus_tag="PLL88_06575"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOJ01270.1"
                     /translation="MKSKMLIMLSCLIVSAMVLTACGSQEATAEPVSEPEAETAAQPV
                     EEGTCPLPVEEGATIVFSGWGDDSEQQIYRDSIERFKTVCPSVTVEYQPVPDEFQNKL
                     KAQMAGGTAPDVFYVDDQLMTAFASSGQLMALDDVMAQAGVSRDDFIPALLTIFTLDG
                     KTYALPKDWGTLGLVYLPAAFADAGIEEPTSDWTWDDMREAAQIIAEKGTYAGYCMAT
                     DWARFAPFAFSNGGKYASDDYTTAALDTPAIKEMATFVTDMFNEGSLVRPADISASWC
                     GEAIGKELVAMTLEGGWMVNSMNLTYPDVEWKTALIPNGPVTRADVIFTNGLGVNAAT
                     EYPMAAAAFAIYVTGSENQGEIVKTGFAYSTHPEQIDLISNVNDKTIAEGGLLPDTLV
                     AYWGPYTSKVNDAVSKALERIWLGEQTVDEAFAQAQAEADTALSGQ"
     gene            11370..12608
                     /locus_tag="PLL88_06580"
     CDS             11370..12608
                     /locus_tag="PLL88_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ01271.1"
                     /translation="MRSPVETPKKGKRILAGILTFLCIFLVIMIVAILNAPTKGGPRV
                     METMQTYLAEVKRTAVPFLIIVGGLAAVMGVIAAITVYRTWRIEKNRKTLFTAYSFLA
                     PYLIITAIFTVGVILFALYISFMKYDIFTEPVWIGFDNYKKAFAGFTDSTQKDFLQAL
                     YNVGWYSLIVVPTQTALAIALAVLLNSRIRMQNFFRTILYAPSVTSSVVITLIFVWLY
                     LKGGFLNGLISAILTPLGVTYDPINWLGDPRGLFQLFGFEIKSAQWYLRGPSIAWMAI
                     MVQNIFTTAPTFMIMFLAALQDINPVLYEAASIDGASSWQQFTKITLPLLRPIILLVV
                     VLGTIGTLQVFDQVYLATQGGPLGTSLTPVYLIYREAMGSQGPINMGYAAALAFILAV
                     IVFLLTFIQRRVLEHGTERY"
     gene            12618..13496
                     /locus_tag="PLL88_06585"
     CDS             12618..13496
                     /locus_tag="PLL88_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ01272.1"
                     /translation="MAHETVKKRKAQRTPGYYLSSILKYLILLLIAAIAVMPFFLATM
                     GTFKTNAEIIAFPPKVFPEKWLWENWSKVWNTDIGSGGTFPRWLFNTAFLSVTVASLQ
                     VICCSMAAYAFARLDFPGKNATFNFMLATMMIPGAVTLIPAYVLMSKLHLINTYWALI
                     IPGAVGASGIFMLTQFFKSIPVDLEEAAVIDGASHMKVFSSVILPLARPSLLTVFILQ
                     FQGMWNAFLQPLLYENSPDMYVLNVALSIFKQQYKAQWNLTLVGAMFNAIPVLILFFI
                     FSRYYIEGVAYTGVKG"
     gene            13504..14169
                     /locus_tag="PLL88_06590"
     CDS             13504..14169
                     /locus_tag="PLL88_06590"
                     /inference="COORDINATES: protein motif:HMM:NF016726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HOJ01273.1"
                     /translation="MQSASADRPSLLQEFHAVFCIIKVSLRDIWEDIWSVLVINLVWT
                     LCVLTIIPGPPATLALFQYANRMAHGETVDVKDFFKGFKGSWGTAWRWGAVNLLVCAV
                     LVGDVVLSNQKNMGASAYPYVQSFYIALLAVWLLLQPYVLTFLLEQEQPLLKTAFRNS
                     FLMLAKNLPFSIAYTLELILTVLVGVVLFMSIFAIGGVLLADVGNRAVLFNLERNKQK
                     LSQ"
     gene            14266..15243
                     /locus_tag="PLL88_06595"
     CDS             14266..15243
                     /locus_tag="PLL88_06595"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOJ01274.1"
                     /translation="MPLTFDFPFEQLKSYLGINPRPDDFDQFWDKGLAEMRGMDAQVA
                     LTPAAFQVPGVECSHLYFSGVDGARVHAKFLRPRQISSPCPAILLFHGYSNNSSDWST
                     KLNYVSMGYVVAALDCRGQGGLSEDRGGVHGNTLHGHIIRGLEDALLGRPEKLLFRNI
                     FLDTAQLAKIVLDMPEVDAERVFATGASQGGALSVACAALEPRVKRIAPIYPFLSDYK
                     RVWMMDYAKDAYVELQEYFRRFDPQHAREDAIFEQLGYIDIQNLAPRIRADVLWGIGL
                     MDTVCPPSSQFAAYNKILSQKRMAIFPDFGHEELPGLSDQIMQFMMNKS"
     gene            15399..15470
                     /locus_tag="PLL88_06600"
     tRNA            15399..15470
                     /locus_tag="PLL88_06600"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15431..15433,aa:Met,seq:cat)
     gene            complement(16105..16611)
                     /locus_tag="PLL88_06605"
     CDS             complement(16105..16611)
                     /locus_tag="PLL88_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01275.1"
                     /translation="MNKRIFIILFTLGVISGCTTSSQLSQVAVQTAQVTQFAPTQHHA
                     VAELQSASASSDKLAFVISISGLDLVKNYSDLDNVICQPYLRTDEHIQFSSYYQESSI
                     PTDPDDPILITYEYGMQHSEVEQLHVHVDLTIGPCGPDLQEMLVTPSPKIDLIANYSF
                     SFVVAVDK"
     gene            complement(16680..17087)
                     /locus_tag="PLL88_06610"
     CDS             complement(16680..17087)
                     /locus_tag="PLL88_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01276.1"
                     /translation="MKNIKFLFVLLLGMLFVGSSPIPVKADSSIPADNEPKVPLDCGV
                     WAYDPVRSGSYVNGTGEISCATVHSSLKVVVGLKDWTNRYTSTTKTCYSTSYCSTTAS
                     LSYSPGRQWITDVSGYMGSYWQAYDVSSWVSIP"
BASE COUNT         4289 a         3947 c         4523 g         4557 t
ORIGIN      
        1 gatgtgctgc gcatgcgcaa cgagctgggt atttccatgc gcgagatgaa cctgaccccg
       61 cataaactgg cagcgttggt gaacctgacg gatgcgggaa aaattaatat caccaccgcc
      121 aagaacctgt tatccaaaat tcaggaaagc ggcaagaacc cccaggtgct ggtggaagaa
      181 ttgggactgg cggtggtaag cgatgatgcg gcgattcgcg cggtttgcga aaaagtcgtt
      241 gcgcagaacc ccaatgaagt gcagtcttat aaaaatggaa aagaaagcct gatcggctgg
      301 ttcgtggggc aggtgatgcg cgaaatgcgc gggaaagcgg accccaggct gacccaacag
      361 atattgaaag aaatactgga aaaataaaaa agctggagag ttttctccag ctttttcgtc
      421 gggttaatct tccaactgtt ccgccaggaa aacctggatt cccatctgtt cgatctggct
      481 taattgtgat tcaagcgtat cgatatgggc ttcttcgtct tttaatattt tttctaaaat
      541 ttcgcgagtg ccgaaatcgt cgaattcgcc ggtgagttta atggcagcat tataggcttt
      601 gatcgcgtct tgctcagcaa tgcgatcatt gtcgatctgt cctttcactt cctttccgat
      661 atgaatgggg ttcagcttct ccacaacggg aacgccctca aggaacaaga tgcgaccgat
      721 gagcgtttca gcgtgcttca tttcatcaat ggcgcgcttt tcagaaattt cgtgcagagc
      781 ttcatacccc caatcggcag ccatttcgct gtgaaccatg tactggttga tggcggtcaa
      841 ttcatcagcc aacaaatgat tgagtgtttc aattactttt tcattgcctt tcatattcga
      901 tctccttcat catgaatttt cattatcaac gaacctatac ctcattatag cgaaaaaacg
      961 ctcgggaaga aaagtgatat caaaagatta acaaattttc gactgtgagg aatgatggca
     1021 aggatatctt gacttttgct tcggaatccg atatagtata gaagaattca tgcgaacgtt
     1081 cgcatcaggg agtaagcagg tgaagcgggt aaccatcaaa gaagttgcag cgaaagccga
     1141 tgtgtcttat cagacggttt ccaaggttat caatcatcag gtggaagtgc tgcctgaaac
     1201 tgctgagcgc atctgggcgg cggtggaaga actgggttac agaccaaatt acacggctcg
     1261 ttccctgcgc tcgaaacgtt cttatactat tggctatacc tggagcccaa ccccgcacgg
     1321 gctgaccaat ccaatccttg accaatttct gcaatccatg ttccacaccg ctgaacaggc
     1381 tgggtactat ttgttatgtt tcccctatca ccaagaaaac gaaaggcaat taaacgttta
     1441 ttccgaattg attgatacag ggagagtaga cggattcatt ttatcttcta ttgaatatga
     1501 ggatcctcgc atcgcctttc tgtttgaccg caacttcccc tttgtggggt ttgggcggtc
     1561 agatgatgac atggtcttcc cctatattga tgtggatggt ggaatgggga tctatcaatc
     1621 tgtgctgcat ctggctgagg gagggcgttg caggatcgca gctcttgcct ggccagagga
     1681 ctcgcgggtg ggcaataacc gtatggcagg ctattttcat gcaatggagg aatgcgggct
     1741 tcccattgat ataagtttga tcgcgcgcgg cgccgggaat tatgaatttg gttaccaggc
     1801 ttgtgacgag ctcatgcaac taccgctctc aatgcgaccc gatgccatcg ttgcgctgaa
     1861 tgattatatg gctatgggcg cgatggctgc catccagaag cagggtctca ttcctgggga
     1921 agaagtagcc gtgaccggtt tcgatgataa cccgatcgca cagtacctgc gcccgtctct
     1981 caccacggtt caacagccca tctgggatat tgggcaaaag gtgattgaaa tgttggtgta
     2041 ccaattggaa gaggggaaaa cgccggatcc tgtttgttcc ctgattcctc ccaggctgat
     2101 cgttcgtcaa tcgagccagc cgaaaggaga aaaaagatag gagaacatag catggcataa
     2161 aggataaatg aataagccac ctctgtggtc ggcaaaccag gtgacttatt ctcaagggat
     2221 tgcgcccttt cgactcagta taacataccg aaactgaaag agaatccccg agtgttgatc
     2281 cataaaattt gcaggcgctg gagggtgcct gggcgcaggg tcttgacttt tcgatacaaa
     2341 actgtattat taacgtaagc gcttacaata aaattacgta aggtaaagag gatggactta
     2401 aaacagatcg ccaaacaaat cggagtgtca actgcaaccg tttcacgcgt gttgaacgat
     2461 caaccgggtg tgagcgcaga ggtgcgcgag aaggtgttga aagcggctgc tgaagcaaat
     2521 taccatccca atcatatagc gcgcagcctg gcaacctcca gatcgcagac gattggtttt
     2581 gtggtccata aagagaaata tgcattttcg gatgaccctt tctaccccct cattctctct
     2641 ggggcagaag agtatctttc gcaacacggc tataacattc ttatcacctt gctcgatgag
     2701 aaagaaaagc agccgcaaaa tttgcccatc attcatcagc gcctggtgga tggactcatt
     2761 ttggcagggc cttttattca agcatcttcc gtcatgagca tcgccagcac aggcattccc
     2821 tttgtgctgg ttgacaattc tctttcgcaa tatccggtca attgcgtgct gaccgatgat
     2881 gaggggggat cttattccgg cgtccggcac ctgattgaag aacatcagca gaaaaaaatt
     2941 gcgtttcttt cgggaccaga tgagtggatc agcaaccgcg agcgattgcg cggttatgaa
     3001 agagccatcc aggaatttga tctggaaccg cgcgtgatcc atgcgaaaga gacaaccatt
     3061 gccagtggag aagagatgat gcggcaggca ttagaacgat ggcccgatct gactgctgta
     3121 tatggggtca acgattccgt ggcgatcggc gctatccggg ctgccgcaaa acggggacgt
     3181 tcaagcccgc gtgatgtggc tgttatggga tttgatgata taagctgggc ggctgccaat
     3241 caaccatcct tgagcacggt caagattttc acgcagcgca tagggtatct ggcagcgcag
     3301 tgcctgctga acgcaaccca gcaaccgggg cttcccccaa ccaagaccct cgttaccacc
     3361 gaactggtca tccgcgaatc ttgtggttgt caggaaaata cttgatgaat aatggaaacc
     3421 gaatgatcga atcgcactta ctccatagat atgcaaagaa ccctgtattg acggcagagg
     3481 cttttccgat gtctgtaaac tccgtgttca acgcaggaat tgtcaaatgg aatgatcaat
     3541 atgtcctctt aacgcgggtt gaggatctca gcggttcttc ttttttgtgg gcagccagaa
     3601 gccaggatgg cttgaacttc acccctgacc ctcgacctgc tttgctgccg gcaaaagaag
     3661 gcgtgtatgc tcctgttgaa gattgcagca tagaagatcc gcgcatcact ccgttggagg
     3721 gatcttttta cgtcacctac gtggggtatt cctgccacga ttgtgtcagc atcctggctc
     3781 gtactgacga ttttttgcaa ttcgaacgtg tctccatcct tggattgccg gaaaacaaag
     3841 atgtggtcct ctttcctgaa aagatcggtg ggcggtatgc aaagttggat cgcccgatga
     3901 cacaaacctc gcagcgcgga gatatgtgga tcagtttttc ccctgatctg ctctattggg
     3961 gtgatcctcg cccggtgatg cagatacgac agcgcaaatg ggattgtttg aagatcggtg
     4021 caggcgctcc tccaatcaaa acatccgagg gctggctggt gatctatcat ggggtgcgcc
     4081 cgacctcagc tggcagcatt taccgcctgg gtgcagcttt gctcgatctg gatcaaccct
     4141 ggcgggtggt ggggcgttct gaacaggcta tcctttctcc catcgcagca gaagattacc
     4201 tgggcaatgt cggcaacgtg gtatttacgt gcggagcgat cctggaagaa aatggtgagc
     4261 taaaggttta ctacggagcg gctgatcagg tgctttgcct tgcttctgct cctgtttcag
     4321 atgtgatcgc tctgtgcctt gaaggaaaaa cagagtgaga atgtgaaaat ggttttgaag
     4381 cggtacgctg caactcctat tcttgaacca gatccccagc atgcctggga ggcgttgaat
     4441 gtgtttaatt gcgctgtggt ggttgcgaac gacctttttc atatgcttta cagggcgcag
     4501 ggggtagccc gtgtgagttc tattggctat gcggtcagca gtgatggcgt tcatttcaac
     4561 cgcttgcagc agccggtcct tgcgccccaa gatgagtggg atgaacgtgg cgtggaagac
     4621 ccgcgggtca cctttttacc cgacgaacgg cgctacatca tggcatatac ggcttactcc
     4681 tctaccggta gcacgccttt ttttgccgag agccgaaatc tgatctcctg gcagcgcatc
     4741 gccccgctgg tggtgagaga agacaacaaa gatcacgtcc ttttcccatg caagataaag
     4801 gggcgttatg ttaccttcac ccgccggatt cccaatatcg ggctggcttt tagcagggat
     4861 ttgaagagtt gggaagattt tcagccgttg atcactcccc gacccggcaa atgggattct
     4921 aaacgagttg gcgcgggggg tccccccatt cagactgaac agggttggct gtgtatctat
     4981 cacggctata acgaggaaaa cagctatcga ttgggaagtt tcttgctgga tcttgacgat
     5041 ccttcccgcg ttctggcgcg ggcagaagga ttcctgatcg aacctcagga accatgggaa
     5101 ttaagaggag atgttccaaa cgtgatcttt tcgactgcca acccggtggt gaatgaaaca
     5161 gtctatgtgt attacggcgg tgcagatcgg gtgatcggtc tggctacctg tgctttgaaa
     5221 gatttgctgg attttacact acacggatga cctgcgatgc atagcgattt aagcaagagg
     5281 agatgatgcg aagtactgct gcaaacaagg atggaaattg gtggagggac ggggtgatct
     5341 atcagatcta tccgcgcagt ttcctggatg ctgatgggga tggggtaggt gacttgcagg
     5401 gcgtgatcga aaaattggac tacctcaacg atggaaccga acgctccctg gggattgatg
     5461 cgatttggct caaccccatt tttccatccc ccatggcaga ttttggctat gacgtatctg
     5521 actactgcga tattcatccc ttgttcggag atctgtctac ctttgatcgc ttgataaccg
     5581 aatctcaccg ccgtgccatc aaagtcatcc ttgattttgt tcccaatcac acttccgatg
     5641 cccatccctg gttccttgaa tcgcgctcga gtcgcgaaaa cccgaagcgc gattggtata
     5701 tctggcgcgc tgccaaagca gatggttcgc cgccaaataa ttggggatct gcttttggcg
     5761 gacctgcctg ggaatgggat gaaacgactg gtcagtatta cctgcattcc tttctcaaag
     5821 aacagcccga tctgaactgg cgcaaccccc aggtgaaaaa agttatgctg gatgttttgc
     5881 gcttttggct ggagcgtggg gtggatggat tccgcatgga tgtgattggt atgctcctga
     5941 aagatgcgga gctgcgcgac aacccccttt cggaagaagc ctcttcttcc gactcagcca
     6001 gtctgtacgc tcgtcagcag catatttatg accaggatca ggatgaggtg catgagatca
     6061 tccgggaaat gcgaaaggtt ttggatgagt accccgagcg ctgtggtatt ggagaggtct
     6121 ggtatgaact cccgcgctat gtacaatact acggcagtga tggggatgaa ttgcatttgc
     6181 ctttcaattt tcgtttgatg aagattccct ggagcgcaca ggcagtcaaa caatcggtgg
     6241 atgagatgga agggatattg cccgatttcg cccagccgaa ttacgtgctg aacaaccatg
     6301 attctccgcg gttgatttca aaaatcggag ctgagcaggc gcgggtggca gccatgctgc
     6361 tattaaccct acgcggaacc cccaccctgt attttggcga agaattggga atggaaaacg
     6421 gtgtcattcc tcccgatcga atgcaggatc cgcagggttt tcgtttgggg gcgaaataca
     6481 gccgagacct gagtcgaact cctatgcagt ggacggctga atccaacgct ggctttacct
     6541 ctggtcagcc ctggctgccg ttgaccccca gttatcagaa caggaatgtg caggcggaag
     6601 ataacgatcc aacctctatg ctcacgctct atcgaaaact gatccatctg cgaaaaaagc
     6661 gcacagcctt gcacggctgc tacgttcccg tttcggtcga gtcggtggat gctttcgttt
     6721 acgaacgctg ccaggagaat gaacgctatc tgatcgcgct gaatttttcc ggagagaaga
     6781 tcacgatgcg cattcctgat ttttctgaag gaaaggttct gctgtcaact tacttcgatc
     6841 aagaaggatg tacggtgacg aacgatctgg aattgagaga gaacgaaggc gtcattatag
     6901 cggtgaaaaa cactgttgca cgacctcagg atgaatgaat catgaataac tggattattt
     6961 ctgaagacag ttttgcagta agtaatttga actcccaaga gacggttttt accattggaa
     7021 atgggtacct gggaacgcgc ggaacgtttg aagaaggctt ttcgggcgag agcgcagcca
     7081 cactcttgca tggggttttc gacgataccc cgatcggttt cactgaactg gcaaatacac
     7141 ccaattggat ggatcttcgt gtgtatgtga atgagcaggt cttcagattg gatgaagggg
     7201 aagtcgtttc gtatcagcgt acattggatc tgcacaacgc aactttaaag cgagagatca
     7261 cctggctttc acctgtcggg aataccttgc gcttcgaatt cgaacggttt tccagcttag
     7321 ccgacgaaca tgtgctggct gtgcgttgcc gcatcacatc ggtagattac aacggtccgc
     7381 ttgagatacg agccagagtg gcaggacatg tagagaatag cggatggaca cattggaatt
     7441 ggcttgggca ggggatgacg gagaacggga tggcatacct ctgcttgcaa accagaaaaa
     7501 ccgaaatcga attgtgcgaa gcgtttaccc tgaaacttgt gaaggataca ccgcagtatg
     7561 aggggtattg ggatagcctg aatgcccctg aaaaagtgct caagacgagc ctacaagccg
     7621 gtcgctcgat ctgtgtagag aagctggttg ctgtatatac cagtcgcgat acgtcagacc
     7681 ctcaaaaagc tgccattgag aagttgcatt ctgctgaggg gaaaggctat gagaaacttt
     7741 gggaagaaca ctgtgcgcaa tgggaagagg aatggaaatc cagcaacgtt ctgatcgagg
     7801 gggacgacaa agctgatcgg gctttacgat atggactttt ccaattgttg atcgcagctc
     7861 cccgccatga tgatcgcgtc agtattgctg cgaaatcgct gagcggtttt ggatatcgcg
     7921 gtcacacttt ttgggatacc gagatcttca tcttgccatt ttttacctat acccgtcctg
     7981 aaattgccag caatttgctg cgctaccgct accataccct ggagggtgcg cgcaagaaag
     8041 ccagagccaa agggttcgaa ggcgcctgtt atgcatggga gagcgcggca accggcgaag
     8101 aaaccacacc ccgctgggca ctgctgcctg atggagggtt ggtgcacatc tggtgcggtg
     8161 acattgaact gcatatcact gtcgatgttg tgttcgctat ctatcagtac tggcaggcta
     8221 ccggcgacga cggtttcatg ctcgattatg gcgctgagat cattctggac accgccgtgt
     8281 tttgggcaag tcgggttgaa tggaacgagg agcaacggca ctatgagatc accgatgtga
     8341 tcggtcccga tgaaaaccat gaacatgtga acaataatgc ctacaccaac aacctggtgc
     8401 gctggaacct gcaaaaaggc atagaagttc ttcgatggat ccaaaacgct gcacctgaaa
     8461 aagccgctga gctggagaag cgattgggga taacttctgc acacttaagc gcatggaaaa
     8521 gcattatcga gaagatctac agcggatttg accagcagac aggtttgttt gaacaattta
     8581 cgggctatta tcagcttaaa cctctggatt taacgtcgct tgaaccgcgc acgagatcgg
     8641 ttcaatccat tttgggaatc gaggaaaccc agaaggtgca ggtgatcaaa caacccgatg
     8701 tgctgatgat gctgtatctc cttaaccacg agtatgatga gagccttcta aaggcaaact
     8761 gggattacta tgcccctcaa accgacctca cgtatggatc ttccctcgga cctgccattc
     8821 aatccatcat ggctacgcgg gtgggggata ttgaagaagc ataccgtctg ttcatgctgg
     8881 cagccgggac cgacctggaa gataagcggg gcaatacagc cgaaggtatt catgctgcta
     8941 cccacggggg gctctggcaa acctgcgtct ttggttttgg cggtgtgagg ttcactgcgg
     9001 atggtcctgt ggcttcccct catcttccta ctggttggaa gcgccttcag tttgagattc
     9061 aataccgtgg ggtaggctat acgttcgacc tgcacgcggg gtcagaacag ccagtgctgc
     9121 cgggtaaaaa agtgccctct gatgagaaac aaaaatcagc catgggtatt tctggggcga
     9181 tatttgatct ggatggggtg atcacagata cttccgaatt ccactacctt gcctggaagc
     9241 ggctggcaga agaagaggga attccttttg accggagcca gaacgatgcg ctgcggggcg
     9301 tttcaaggcg agaatcgctg cagaagatct tgggtgatcg tacggtatct gaagaacaaa
     9361 tgcaagccat gatggaacgg aaaaatggct actatctgga ttatctgaat agcctcactg
     9421 aagcagatct gctgcccggc gttctggatt tcatgaaaga tgcaaagcgg caagaggtca
     9481 aactggcagt gggttcagcg agcaaaaaca cgcaacttgt gcttgaaaag ttgggaatac
     9541 gcgacctttt tgatgcgatt gcagacggat tcagtgtggt gcatgtgaag cctgctcccg
     9601 atttgttctt gcatgctgct gaccaactcc aggttgaacc tgaacattgt attgtatttg
     9661 aagatgcaga agccgggatc aaggcagccc tggatggaaa tttttgggca gtgggcgttg
     9721 gtccgcagga gcggctacat gctgcgcatc tggtgatttc tgatttcaaa ggtttgaatt
     9781 ggggaacctt tatggatcaa atggaaaaaa cgcaaaagaa catatagcga acctgtataa
     9841 gcgggtgaat aaaaaggatg aaggaagtga aagaaaggag gtggatatat agaaaggacg
     9901 aaaagaaagt cttgacaaac tatgtggttt aaaaattgtt aaaaaaggag agatacatga
     9961 aaagcaagat gctcattatg cttagctgct tgatcgtgag cgccatggtt ctaactgcat
    10021 gtggttccca agaagcaaca gcggaacccg tcagcgaacc agaagcagaa actgcagcac
    10081 aacctgtaga agaaggcacg tgcccattac cagtagaaga aggagcgacc atcgtcttct
    10141 ccgggtgggg cgatgattct gaacagcaga tctacaggga ttccatcgag cggttcaaaa
    10201 ccgtttgccc atccgttact gtggagtatc aaccggttcc ggatgaattc cagaacaaat
    10261 tgaaagcgca gatggctggt ggcacggctc cggatgtgtt ctatgtggat gaccagttga
    10321 tgaccgcttt tgcctcttct ggtcaattga tggcattgga tgatgtgatg gcgcaagccg
    10381 gtgtcagtcg cgatgatttc atccctgcct tgttgaccat cttcaccctg gatgggaaaa
    10441 cctatgccct accgaaagat tggggcacat taggtttggt ctacctgcct gcggcatttg
    10501 ctgatgcagg cattgaagag ccaacttctg actggacatg ggacgacatg cgcgaagcgg
    10561 cacaaatcat cgctgaaaag ggcacctatg ccggatactg catggcaact gactgggcgc
    10621 gctttgcacc gtttgccttc tcgaatgggg ggaaatatgc ttcggatgat tacaccactg
    10681 ccgccttgga tacgccagcc atcaaggaaa tggctacctt tgtaacggat atgttcaatg
    10741 aaggaagcct ggttcgccct gccgatatca gcgcaagctg gtgcggtgaa gcgattggga
    10801 aggaattggt tgcgatgact ttggaaggcg gttggatggt gaattccatg aatctgacct
    10861 atcctgacgt agaatggaaa acagccttga ttccgaacgg acccgtaacc cgtgcggatg
    10921 tgatcttcac caatggactt ggcgtgaacg ctgccacaga ataccctatg gctgctgccg
    10981 cttttgcaat ttacgttacg ggcagcgaaa accagggtga gattgtaaag acgggctttg
    11041 cctacagcac ccatcctgaa cagattgacc tgatcagcaa cgtgaatgat aaaaccatcg
    11101 ccgagggtgg tcttttgccc gacacactcg ttgcctactg gggtccttat accagcaagg
    11161 tgaatgacgc tgtatcaaag gctcttgaac ggatttggtt aggtgaacag actgtggatg
    11221 aagcttttgc gcaagcgcaa gctgaagccg acactgccct gagcggacag taaccccatt
    11281 tttctcccgg gtctggcggg taccctagag tacccgccag atcacctttc ttgattataa
    11341 cgtatctgtg tgaggcaagg aggtgtacca tgcgatctcc tgttgaaacc cctaaaaagg
    11401 ggaaaagaat tctggctgga attctcactt ttttgtgcat ttttttggtg atcatgattg
    11461 tggctattct caatgcccca accaaaggtg gtccaagggt tatggaaacc atgcaaacct
    11521 atttggctga ggtaaagcga acagccgtac cttttcttat cattgtgggt ggactggcgg
    11581 cggttatggg agtgatagct gccataacgg tgtatcgtac ctggcgaata gaaaaaaaca
    11641 gaaaaacttt atttactgcc tattcgtttt tggctccata tctgatcatt accgcgattt
    11701 ttacggtggg tgtgattctc tttgctttgt atatttcgtt catgaaatat gacattttca
    11761 ccgaaccggt ctggatcggt tttgacaatt acaaaaaagc ttttgcaggt tttacggatt
    11821 caacccagaa agattttttg caggcattgt acaatgtggg ttggtattcg ctcattgttg
    11881 ttcccacaca aacggctctg gcaatcgcac tggctgttct gttgaattcg cgcattcgca
    11941 tgcagaattt tttcaggacg atcttgtatg cgcccagcgt aacttcttct gtcgtgatta
    12001 cgttgatctt tgtctggctt tatctaaagg gaggcttcct caatggcttg atcagtgcta
    12061 ttctaacccc tcttggcgtt acctatgatc cgatcaactg gctgggggat ccgcgagggc
    12121 tttttcaatt gtttgggttt gagatcaaat ctgcccaatg gtatctcaga ggacccagca
    12181 tcgcctggat ggctattatg gttcaaaata ttttcaccac cgcgcccacc ttcatgatca
    12241 tgttcctggc tgcgctgcaa gatattaacc ctgttttgta tgaggctgct tcgatcgatg
    12301 gggcaagcag ttggcagcag ttcacaaaga tcacgctacc gctgttgcga ccgatcattc
    12361 tgctggttgt tgtactgggg accatcggta ccttgcaggt gttcgatcag gtttacctgg
    12421 caacccaagg aggtccgctg ggcacatccc tcaccccggt ctatttgatc taccgtgaag
    12481 cgatgggttc gcagggaccg atcaatatgg ggtatgccgc ggcgctggcg ttcatcctgg
    12541 cggtcatcgt ttttctgctt actttcattc agcgacgcgt tctggaacat ggcacggagc
    12601 ggtactagga ggtagaaatg gctcatgaaa cagttaaaaa gagaaaagca cagagaactc
    12661 ccgggtatta tctttcgtcg atcttgaaat atttaatctt gttgttgatc gccgctatag
    12721 ctgtcatgcc attctttctg gcaactatgg ggacattcaa aaccaatgct gagatcatcg
    12781 catttccacc gaaggtcttt ccagaaaaat ggctgtggga gaactggtcg aaagtgtgga
    12841 atacggacat tggcagtggc ggaaccttcc cccgctggtt gttcaacact gcttttctgt
    12901 cggttacggt ggcatctttg caagtgatct gctgttccat ggctgcctat gccttcgcca
    12961 gactggattt tccggggaag aacgcaacat tcaatttcat gctggctacc atgatgatcc
    13021 ccggagcggt gaccctgatt ccggcatatg ttctcatgtc taaattacat ctcatcaata
    13081 catactgggc gttgatcatt cctggcgctg tgggagcgag cggtattttt atgctgacgc
    13141 agttctttaa atccatcccg gtagatctgg aggaagctgc ggtgatcgat ggtgccagcc
    13201 acatgaaagt gttctcgagc gtgatcctgc cgctggcaag accgtcactg ctgaccgttt
    13261 tcatccttca attccagggt atgtggaatg ctttcttgca acctctcttg tatgaaaact
    13321 ctcctgatat gtatgtgttg aatgtggctc tcagcatttt caagcaacag tataaagccc
    13381 aatggaatct gacgctggtg ggcgccatgt tcaatgccat tccggttctg atcctcttct
    13441 ttatcttcag ccgctactat attgagggtg ttgcgtatac aggcgtgaaa ggttaagtta
    13501 tccatgcagt cagccagcgc agaccgccct tcgttgctgc aggaatttca tgcggtattc
    13561 tgcatcatta aggtatcctt gcgcgatatc tgggaagata tctggtctgt gctggtcatc
    13621 aacctggtgt ggactctgtg tgttctcacc atcatccccg gccctcccgc cacgttggct
    13681 ctttttcagt acgccaatcg tatggcgcat ggcgagactg tggatgtgaa agattttttt
    13741 aaaggattca aaggatcatg gggaactgcc tggcgatggg gggcggtaaa tctgctggta
    13801 tgcgctgttc ttgtagggga tgttgtattg agcaaccaga agaacatggg tgcctctgca
    13861 tacccttacg tgcagagctt ttatatcgcc ttgctggcgg tgtggctgct gctgcagccc
    13921 tatgtgttga cgtttctgct cgagcaggaa cagccgttgt tgaaaaccgc ctttcgcaat
    13981 tccttcctca tgctggctaa aaatttgccc ttttccattg catatactct cgaactgatc
    14041 cttaccgttt tggtgggtgt ggtattgttc atgtccattt ttgccattgg cggggtgttg
    14101 ctggcggatg tgggaaatcg ggcggtgctt ttcaatctgg aacgcaataa acaaaaatta
    14161 tcccagtgaa ttttaaactg tgctgcattg tttcagcaga acatcgatat tcattctgtg
    14221 tgtttttccg ttctgatatt aaaatactgt caaggaaggg tagccatgcc gctcacattt
    14281 gattttccat ttgaacagct taagtcatac ctggggatca accctcgccc tgacgatttt
    14341 gatcaattct gggataaagg gttggcagaa atgcggggga tggatgcgca agttgcgctc
    14401 accccggcag catttcaggt gccgggcgtt gaatgctcac acctgtattt cagcggggta
    14461 gatggggcac gggtgcatgc caaatttctc cgcccgcgac agatttcctc accttgccct
    14521 gccattcttt tattccatgg ctattccaac aattcgagtg actggtcgac gaaactgaac
    14581 tatgtatcta tgggatacgt ggtggctgcg ctcgactgcc gcgggcaggg agggttgtcg
    14641 gaagatcgcg gaggagtgca tggaaatacc ctgcacgggc acattatccg cggtctggag
    14701 gatgcgctgc tgggcagacc cgaaaaattg ctttttcgga acatctttct ggatacggcg
    14761 caactggcga agatcgtcct ggacatgccc gaagtagatg ctgagcgggt ctttgccaca
    14821 ggagcgagcc aggggggcgc cttgagcgtg gcatgcgctg cgttggaacc acgcgttaag
    14881 cgcattgctc ccatctatcc atttttgagt gattacaaac gggtgtggat gatggattat
    14941 gccaaagatg cgtatgttga gctgcaggaa tattttcgcc gtttcgatcc gcagcatgct
    15001 cgcgaagatg ccatcttcga gcagttggga tatatcgata tccagaactt ggcgccgcgt
    15061 attcgagccg atgtgctgtg gggcattggt ctcatggata cggtatgtcc tccatccagc
    15121 cagtttgctg cctacaataa gatcctttct caaaaaagga tggcgatctt ccctgatttt
    15181 gggcatgagg aacttcccgg gttgagcgat cagatcatgc aattcatgat gaacaaaagc
    15241 tgaatcggat tgttgacgat aagggcgaaa acatgcgacc atccaacgtg tcaggaaggg
    15301 attcattcga aagttcgtgc cacaattctg ccagaaagac catccctgag agctcgcttg
    15361 actcataacg ccgcccgtgg taaactagct tctcacgttg cggggtagag cagtggcagc
    15421 tcgtcaggct cataacctgg aggtcgttgg ttcgaatcca acccccgcta ctaagcatga
    15481 accagagtcc gtcgggctct ggtttttcgt tataattatg tagtatagag gtgagtgatg
    15541 aacgagatgg cgcacgaaaa gaaattactt aagaagattg atgtactatt ggtaattttc
    15601 ctcttactct tgcctggaat acggggttac agtgactggc atttttatga atatgcctta
    15661 tttattgttc ttccattgct ggggttgttc ttccagcgag taaatgtgaa gaaaatttat
    15721 taaatatcac ttcatcgaat tttccacagt ttttaaagca atgttggcaa tgctctctga
    15781 gcacactgct tggcatatga gagtgtttgg ttttccaatt gtatgcgttt ctaaaagcac
    15841 caaaaacttt tgtatttgtg ctttcacttt tttcatttcc tgcgagactg gtttatcgat
    15901 tggttcaccc catggcgatt ttcccaatcg tatgaactaa tacatggagt gatgcttgta
    15961 tcttcgagcg ctgagctccc tccgaatgac cgtctactga cctcgacttc tacatagggt
    16021 tgcatccatt tctgattctt attaatacca ggacgacagg aggtttctgg atttttgaaa
    16081 atcgcccgat cgaccaagac ggatttattt gtctaccgcc acaacgaagg aaaaactgta
    16141 atttgcgatc aggtcaatct ttggggaagg agtgaccaac atctcctgca gatcgggtcc
    16201 gcaaggtcct atcgtcagat ccacatgcac gtgtaattgt tctacctcag aatgttgcat
    16261 tccatattca taagtgatca aaataggatc gtctgggtca gtcggaatgc tgctttcctg
    16321 ataataggat gaaaactgga tatgctcatc ggttctcaag tagggttggc aaatgacgtt
    16381 atcaaggtca gaataatttt taaccaaatc caaacctgaa attgagataa caaaggcgag
    16441 tttatcagag ctggcacttg ctgactgcaa ttcagctacc gcgtggtgtt gcgtgggagc
    16501 gaactgcgta acctgtgcag tttgtaccgc tacttgcgat agttgacttg aagttgtaca
    16561 accagaaatg accccaagag taaataggat tataaaaatt cttttattca tcgtttcacc
    16621 attacatgtg gtagcgacaa tgttttttta ttttatatcg ctaccacagt tgtcaacttt
    16681 cacggaattg aaacccagct agaaacatca tatgcctgcc agtatgaccc catatatccc
    16741 gaaacatcag ttatccattg tctacctgga gaatatgata gtgaagcagt tgtcgaacag
    16801 tagcttgtac tatagcaggt tttcgtagta cttgtgtaac gattggtcca atctttcagt
    16861 cctacaacca ctttcagact cgaatgtact gtcgcgcaag atatttctcc tgttccattg
    16921 acatatgaac cggagcgcac aggatcataa gcccaaactc cgcaatccaa tggaactttc
    16981 ggttcattat cagcaggtat tgatgaatcc gcttttacag gtatcggtga tgaacctaca
    17041 aaaagcatgc ccagaagcaa gacaaaaaga aatttaatgt ttttcatttc cctctccttg
    17101 tttatttagt cttcctattt gttgaaatga taggaagaca ccatacaaaa cacggtattg
    17161 ttgaccttca cctataattt gttgtacgaa taaaggggaa gttaaattat aatacttgct
    17221 tttatatttt caagtgacat tttttcactg ttatttgaaa ctgaaaagtg aaccgttagc
    17281 atatcaaaag tgaacttctg atccatgata gatttc
//
LOCUS       DAOSDS010000045        15941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1487_length_15941_cov_4.637794, whole genome shotgun sequence.
ACCESSION   DAOSDS010000045 DAOSDS010000000
VERSION     DAOSDS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15941
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1487_length_15941_cov_4.637794"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLL88_06615"
     CDS             <1..202
                     /locus_tag="PLL88_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01277.1"
                     /translation="MYGFNRCERERGHPWVMSLNILGRGEIGKGAMRTDMVVNPLPLD
                     QGQIEGGEVIIELIDSISLEKK"
     gene            199..1128
                     /locus_tag="PLL88_06620"
     CDS             199..1128
                     /locus_tag="PLL88_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ01278.1"
                     /translation="MNEEKKVLSEERGIIRVCSLLDWANGFILDRKARGLAQGTIRFY
                     QKKLSKFIDYCFLNEAHDMESLNSSLVRDYFLWLQERENHTQGGIHAYYRTVKTFLRW
                     YERENDLPGWANSVLKIKIKQPQLIPLEPADVGAIKKMIACAGSRDKTILMVMMDTGI
                     RASELLALNLENVNAITGVVQVLHGKGGKFRTVYIGRKTRIALRRYLNGRTDENPALF
                     INRHKERLLPSGLRQILCRLSERAKVEYQPPHSFRRLFAITMLRNGVDIYTLQLLMGH
                     EDIQVLKRYLRVTQDDALRAYNSGSPVDKLLSR"
     gene            complement(1190..1483)
                     /locus_tag="PLL88_06625"
     CDS             complement(1190..1483)
                     /locus_tag="PLL88_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01279.1"
                     /translation="MGERTLVKDLVPVLDDHGKRIEKLEKSKDCFDDHLQKHADVYDE
                     KLKNHQEVLFGVKGDDGLVFDVKQIIQMKKNLNNLTWAVITAIVIEVALRLLK"
     gene            complement(1488..2480)
                     /locus_tag="PLL88_06630"
     CDS             complement(1488..2480)
                     /locus_tag="PLL88_06630"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HOJ01280.1"
                     /translation="MILGTDISHWQTNDASNPQHFFDPVIAKEKGVEFAFLRASDGAT
                     NDKAFQTFARTFKQAELPFGVYHYARPAGTIYGTALQQADRFWNLIKDTGFTLSPALD
                     LECQGVGLSFTKVFLERIKELSGRTPIIYTSPAFWTGLKDSGNALWAADYPLWIAHYF
                     SSLIFPQYGIPEKVINSTTLPSVPNPWKKKDKMWSIWQFCATGDGEYYGGDYAKHSEN
                     KVGLDLDVYHGTLEELLDELGNGTESGEVIQPSEPFPKRVKIISAFLRGRSQPRYFEG
                     ISAVIFEKGQILYRTDEEPVYEPASGITWIQVQVPDCPCCGMWISSNPKYIKEI"
     gene            complement(2490..3194)
                     /locus_tag="PLL88_06635"
     CDS             complement(2490..3194)
                     /locus_tag="PLL88_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01281.1"
                     /translation="MNFESDPGDGGIYGFSASYAIARLTSSAYFGGEFFTVGQAQTGG
                     AVSYVYRSYLKFDTSPISPTATILQVNLRMVAILVNVYTGTDFDIQIIKCDWSAFDPV
                     DDTNRENVYDLILSENQDNSIWRNTSGMAIDTQYTSGNLDTDWINKDGYTYYGLRSSR
                     DFNSVGPSLTRNELVRIGSGDNATEDYRPALIVSVEEPGAFVPRTMIFFDRSFDWGWR
                     KKDGLWQPQNPGLVTI"
     gene            complement(3198..4127)
                     /locus_tag="PLL88_06640"
     CDS             complement(3198..4127)
                     /locus_tag="PLL88_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01282.1"
                     /translation="MGKKYTKNTWTDEELVDDPLYNILQSDDTPINEEVKIELSTEVI
                     AAGSPVNASRMNNIENGIDALDDALDLENAPEKEEPIAGDLLLLADSEDDYAHKSVDV
                     SALGGGNAPETTAENDFQVGNGLGAWVKKTLAEVLTILGKAAANGLASLDASSKVIQD
                     PANATSTPTASKIPIADAGGKLDGWVKRIVEWRVIGKDAALTTGDGKDYFIVPADLDG
                     YKLVDFDVAVDTVSSSGAPTVQLHNATDNQDVLSTPATIDASEYTSYTAATPPVIDTD
                     HDDVATGDRLRCDVDAAGTDTKGLTLIMVFQEN"
     gene            complement(4138..4821)
                     /locus_tag="PLL88_06645"
     CDS             complement(4138..4821)
                     /locus_tag="PLL88_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01283.1"
                     /translation="MSKKFSDFESNPDTVLTTPAPGDLCLIYDQSEPLEVNKIKVISY
                     QNLVNLAAQAALQALVSGQAAGDLFYASGPASLARLAKGTAGQSLKMNGGGTAPVWGS
                     SLSCFGSVYKSSTDIATTTYADIPDVKIDLTVPCTSTLVAILGFHGLKYGTSGRLFVR
                     WSIDDTTLNEVSLESHSASWSHPVTVIGFKQSVAVGSRVCKVQAYAAAAPNYGYMESI
                     TGIVLGFAE"
     gene            complement(4830..6203)
                     /locus_tag="PLL88_06650"
     CDS             complement(4830..6203)
                     /locus_tag="PLL88_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01284.1"
                     /translation="MITKPLIRLTVGSETLEFSGEEVTSATLVEETNIIGMELPISAL
                     EAKIMNYDESFSMFSGSYFSLLSERLPVVAYENVDGELQFLGKFYLSEWKNTSEHEFE
                     FRAVDIIGILDATDYDGNFWSADTPLGTVLAQTFVPINVLYTLDESLQSVPIRGWIPP
                     GNYREALQQICLAAGAAAISVRSDRLQIRPVVIPTQHFDYRVRDTDKLMEQSIELQTL
                     VTSIELVSHNYTQGSELEEIFNKELEAGSHKIIFEKPYYNIVVDGPGYDPSYLVTEDE
                     EYIITEAGDQIEVGGEYVFGPNVLYLEMTQSGIITITGYPWLDSKRAYIFRETGITEY
                     ANKNALVVDSATLVNMDNAQIILDRVRDYYRQRYRKTITLLPSEITLGDIVLTRTLYQ
                     KNILATVQKMSTDLAGGYLAQTELLGIEPAYIEPVDHPARRARSGVATCGADLTRQNM
                     FREYDHE"
     gene            complement(6200..6568)
                     /locus_tag="PLL88_06655"
     CDS             complement(6200..6568)
                     /locus_tag="PLL88_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01285.1"
                     /translation="MIIIDGVEYDIPVISIERTADFLDKFAERTVDGVLHRELIGVYF
                     NYRLQLGSITDTAVYAALWAKLTEPQEFHTVTVPDESGNYTFTAYFSNVGDKLRKSQG
                     IQNYWKGLTVNFIARSPART"
     gene            complement(6565..8193)
                     /locus_tag="PLL88_06660"
     CDS             complement(6565..8193)
                     /locus_tag="PLL88_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01286.1"
                     /translation="MAAGYDGTIRIDTRVDEKGFNEGVKRLGGTLKNIATAILSLLKT
                     AAIGIVVLSVAFVGLIAVVGKWSQELTETLHKNLSVTTAMRDRVVQLKGAFDGLKGAV
                     MATGATLLNALAPVLLKIIDWLVKAINWVTMFVAALTGQKTVMQYVSGAVDSAAGSTA
                     KLAKATKDTEKAAKGALAAFDEINVLQMESADEADTGGGAGGNVIMREVAVPEDFIKD
                     SWIGFKQWFKEDIIDVMSGWLADVWKNPFAEGTNPIQNLLETLGLEENPIKLFWEWGI
                     SEGTNPIQNLLTLLGINASNPLRLPWKLAEPGTNPIQNLLETLGLEENPIKLLWELGT
                     KDGSNPIKNLLDETKRRFDEFDWNALWTDLQIGAANAVLRVIESVSNLKARINETFDG
                     IKNFAKGTINSIIDFINGMIRGITTGINAVIRALNTIKIDIPATFLTPAYSFGLNIPT
                     VTAPQIPRLATGAVIPPNAEFAAILGDQRSGRNLEAPEGLIRQIIQEELGNIQTDIEI
                     KFAGSLGALVRELKPYIDKENTRIGNSLVRRSAA"
     gene            8262..8525
                     /locus_tag="PLL88_06665"
     CDS             8262..8525
                     /locus_tag="PLL88_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01287.1"
                     /translation="MSKTTTAGAKTEVRVEIYKNKHDLKKGMKKEQDLGWTVLSVQNV
                     PQGYGCFKTGCLGLVFLPLALLGRKPEKYQVTYQRQIAQNPNH"
     gene            complement(8515..9144)
                     /locus_tag="PLL88_06670"
     CDS             complement(8515..9144)
                     /locus_tag="PLL88_06670"
                     /inference="COORDINATES: protein motif:HMM:NF018553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage Gp15 family protein"
                     /protein_id="HOJ01288.1"
                     /translation="MNILTESLPLAIEIDGIEYEINSGFRSCLRIILAFEDNELADAE
                     KRMILLNNLYKSMPHDIPQAIKKGIRFLDGGKDSGNGESNGESELRLYSFEQDASYIM
                     AAFKQTHGIDLDAVEMHWWKFLALFMDLGSETTFSSLVSLRKRVKTGKATKEERAVAR
                     EIKEIFDLPDIDRRTLEEREAEAHFMELVATGKKQHKQDIEYPEEAIND"
     gene            complement(9141..9575)
                     /locus_tag="PLL88_06675"
     CDS             complement(9141..9575)
                     /locus_tag="PLL88_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01289.1"
                     /translation="MNTLKISGIKRIAINDDPNNVIEFNPTDVLFVERFYAMCQEFQV
                     RLEEFDQRYAKLNEVGLDENGMLTDANDNFALQKEFCTYVRRKIDDLFGNGTSQKVFG
                     DALSVDMITDFFEGITPFIWDARNERVKKYSNSVTDKRRSMK"
     gene            complement(9584..10075)
                     /locus_tag="PLL88_06680"
     CDS             complement(9584..10075)
                     /locus_tag="PLL88_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01290.1"
                     /translation="MARIDRTQFVTFLNVGTQSMPDYALVGQGITSAPVNYQPQTEEE
                     QYIHQKSGTTSIVGYRPTMPIEMKMDTDDEVCMFVDGLRRERATLSDAETDIIMVYLY
                     ETPVGGEYPAEKQTVQVQIDEFSGEPGANPLNFTFLFQGDAVQGMFDPTDATFTSESE
                     GSS"
     gene            complement(10090..10539)
                     /locus_tag="PLL88_06685"
     CDS             complement(10090..10539)
                     /locus_tag="PLL88_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01291.1"
                     /translation="MSIISAIQDYIKTYTSLKHGAPVWVDFLGPEPTQYAITPLPGTR
                     VLEEDIVGNKIMSYPFAFQTMESTADDLERLETQGFYEAFAEWLELQSESGNPPVLGE
                     RQIAESIEATGWGYLYEQGDSQTGVYQVQCRLVYTQKPREEESEESS"
     gene            complement(10536..10934)
                     /locus_tag="PLL88_06690"
     CDS             complement(10536..10934)
                     /locus_tag="PLL88_06690"
                     /inference="COORDINATES: protein motif:HMM:NF022564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HOJ01292.1"
                     /translation="MAKGPVIKTPRGRIFINKNGKAELVWNTNFQPKWQKRYSEAQKF
                     VDSEVLRLCEPYVPLRTGMLIKSGILGTEIGSGEVKYIAPYARRQYYSRRKPGSQTGP
                     LRGPYWFQRMKEVHGRRIIEGARRIAGGGK"
     gene            complement(10934..11281)
                     /locus_tag="PLL88_06695"
     CDS             complement(10934..11281)
                     /locus_tag="PLL88_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01293.1"
                     /translation="MRTNTAATLYSRSVVSGSEVWARSVIPAVFWEQRKAANVIKSGL
                     LEADKAAVYIPDISIAIKIGDMLVKGTADKVISPSYTTSNLRADYEVIQVRSVDTMDY
                     GSSHMQHIQVGGS"
     gene            complement(11271..11681)
                     /locus_tag="PLL88_06700"
     CDS             complement(11271..11681)
                     /locus_tag="PLL88_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01294.1"
                     /translation="MTAYANWEFYSTTYLGTAIAQAAFPALALRASAKIDQITFDRAA
                     AIYEAAEDTETITKIKLATCAVADEMQRQISQPREVASERVGNFSQNYIVTKDMELTS
                     DQRLANAAKLHLGSTGLMFAGFAAGEYGGAANAN"
     gene            complement(11678..12307)
                     /locus_tag="PLL88_06705"
     CDS             complement(11678..12307)
                     /locus_tag="PLL88_06705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ01295.1"
                     /translation="MISLIIVANNKWGDFATPYIESVLRYEPNTEIILVDNGSPEPYP
                     SSCDYHLIRIDPTEHYNYMAALNAGARAAHGDWLMFSNDDVLCKGAFAKTIESQSFSW
                     LYGMEVREKPAKWGAGKDFCYVYGWLLIMHRAVWEIVGEFDEYYLHAGFDDLDYSWRC
                     QEKGVPLKILDLPFVHLADQPGNFHRRMTVEGYKENMKRSKAHFLEKVL"
     gene            complement(12304..12879)
                     /locus_tag="PLL88_06710"
     CDS             complement(12304..12879)
                     /locus_tag="PLL88_06710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ01296.1"
                     /translation="MSVNFTKRVIDLPNLKNINKAIPGGRVGVKTIEGCRLAKLAHDR
                     ETIVEIGAATGRSAAFMASGLRYARTKGTIYSVDIWDDEKWLADYKANLKKLGLLDYV
                     VPIRKSSAEAAAEWDKSIDLLFIDANHSYAHVKQDFELWFPLVVKGGLIAFHDYGSEA
                     WEGVTRFVDSIPNNKLRCIGVHRTLWAGEVQ"
     gene            complement(12876..13721)
                     /locus_tag="PLL88_06715"
     CDS             complement(12876..13721)
                     /locus_tag="PLL88_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01297.1"
                     /translation="MIDHEWDGLFAGYIPTKFRSQIAECEQLGIPVFVYPHSVRPNIP
                     FDLTREFHPNIRALFTIAEGHKVVLKKLGYPNPIEVVGWPYTEICSFRCNSSIEKVRV
                     LFAPLHPVGGSFLPDIDRELNVKTYKLLLGLLDRIDLTVRHIGSLKYNNIEPDQRVKF
                     IAGQMDGSTDDMDNADVVIGAYTYAYMAVALGHPLVMLGEGVRPHNSPRSGGQLLWAK
                     NWEKYRDYMAYPFNVEDCQTSEDLYDMLKLAMGGDQGIDNWKQRFIGEPFNGQSFVKT
                     LESYL"
     gene            complement(13837..14199)
                     /locus_tag="PLL88_06720"
     CDS             complement(13837..14199)
                     /locus_tag="PLL88_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01298.1"
                     /translation="MKFENLADFVDLFGKVLIFAGLIEAVVQNLKLVYDNKTFRPNPD
                     VLVSLAVSVFACVFFKIDIFKLAAFNSLWEPLGSIFTGILISRGAGALHDLLELVFYK
                     SYQALHEKEVARKTSAKS"
     gene            complement(14254..15219)
                     /locus_tag="PLL88_06725"
     CDS             complement(14254..15219)
                     /locus_tag="PLL88_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01299.1"
                     /translation="MALTLAEAKQLSQDKLTNIVIDEFRKSALLDVMIFDDTVKPQGG
                     ESLTYAYNRITTQATAAGRAINSEYGAQETKTTRYTVDLKPFGGSFNLDRVIINHETQ
                     VVNHIQFQIEQKIKATRALFADWFINGNSATPTQFDGINKAVTGSSTELEPTTTIDLS
                     TSSNIDTNWKVFLDHLRKLRAKLDGAPSLYMMNTDMFGIFQSVMDRAGINLANKENYG
                     DEVAVWGPSLVMALGDKPGTANPIIGVDAYGETSIYCARIALDGVHGVSPEGSPLVQI
                     YLPDMTVPGAVKLGEVEMVAAVAVKATKSAGVLRGVQIVANGASS"
     gene            complement(15228..15809)
                     /locus_tag="PLL88_06730"
     CDS             complement(15228..15809)
                     /locus_tag="PLL88_06730"
                     /inference="COORDINATES: protein motif:HMM:NF018515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage scaffolding protein"
                     /protein_id="HOJ01300.1"
                     /translation="MNKKDLEKLGLTAEAIEKAGLGSDVLDQIIILHGKDIEKHKTDL
                     SAAQSELDGAKKQLEEANKTIESFKAMKPEELQKAADDWKAKAEVAKAEADKTIAGLK
                     FDHALDGALTTAKAKNAKAVKALLKTDDLKLADDGSIVGLNEQLEKIKSENDYLFESD
                     TPDPKIITGGKQTTDAPTGSFAEAIQERLSKKK"
BASE COUNT         4007 a         4001 c         3425 g         4508 t
ORIGIN      
        1 aatgtatggg ttcaatagat gtgagaggga gcggggacat ccctgggtaa tgagtctgaa
       61 tatactcggc aggggagaga ttggcaaggg cgcgatgcgg acggacatgg ttgtaaatcc
      121 gctcccactc gatcagggtc agattgaggg tggggaggtg ataatcgagc tcatagacag
      181 catttctctt gaaaaaaaat gaacgaggaa aagaaagttc tgtctgaaga gcgagggatt
      241 attcgagttt gttctttgct ggactgggct aacgggttca ttttggaccg caaagcacgg
      301 gggctagcgc agggcacaat tcgtttttac caaaagaaat tatcgaaatt cattgattat
      361 tgtttcttga atgaagcaca tgatatggaa tcgctgaatt caagccttgt cagggattat
      421 tttctgtggc tccaagaaag agaaaaccat acacaaggag gaatccacgc atattacagg
      481 acagtcaaga catttttgcg ttggtatgaa agagagaacg atctgcctgg ttgggctaat
      541 tccgttttga agataaagat aaagcaaccg caattgatcc ctcttgaacc ggcagacgtt
      601 ggagcaataa agaaaatgat cgcgtgtgct ggctcgagag acaaaacgat attgatggtt
      661 atgatggata ccggtatacg agcttccgaa ttattggcat tgaaccttga gaatgtaaat
      721 gcgataacgg gagttgtgca agtattacat ggcaaaggcg gtaaatttcg aacagtatac
      781 atagggcgta aaacaaggat cgcgttgaga agatatctaa acggtagaac ggatgagaat
      841 ccggcattat ttatcaatcg gcataaagaa cgattattac catcgggact gaggcaaata
      901 ctgtgcaggt tatccgaaag ggcaaaggtt gagtatcagc ctccccactc tttccgaagg
      961 ttgttcgcaa ttactatgct ccgaaacgga gtggatatat atactttgca attactcatg
     1021 gggcatgaag atattcaggt gctgaaaagg tatttgagag tcacccagga tgatgcatta
     1081 agggcatata acagcggtag cccagtggac aaattgctgt cccgttagtt cagaatcact
     1141 tttaataaaa ttacatgaaa tatttcaagc gaattggtgt acttcgtttt catttcagca
     1201 aacgtagtgc aacctcaatc acgatagctg tgatcaccgc ccatgtcaga ttgttcagat
     1261 tcttcttcat ttggatgatt tgtttcacat caaatactag cccatcatca cccttcacac
     1321 caaataaaac ttcctgatgg tttttgagct tctcatcata aacatcagcg tgtttttgca
     1381 gatggtcatc aaagcagtct ttggactttt ctagcttctc tatgcgcttg ccgtgatcgt
     1441 ccagcaccgg aaccaggtct tttacgagtg ttcgttctcc catgtgctta tatctccttg
     1501 atgtattttg gattggagct gatccacata ccacagcatg gacaatcggg tacctgcacc
     1561 tgtatccaag tgataccact ggctggctcg tacaccggct cttcatctgt tcgatagaga
     1621 atttgacctt tttcaaagat cactgctgat ataccttcaa agtagcgcgg ttgagagcgt
     1681 ccccgcaaga aagccgaaat gatctttacg cgctttggaa atggctcgga cggctgtatc
     1741 acttccccgc tttctgttcc atttcccaat tcatccagta attcttctaa cgtgccatga
     1801 tagacatcta gatcaagacc aactttgttc tcgctatgct ttgcataatc gccgccataa
     1861 tactcgccgt ctccggtcgc gcaaaattgc caaatagacc acatcttatc tttctttttc
     1921 cacggattag gaacactcgg caatgtggtc gaattaatca ctttttctgg aattccgtat
     1981 tgggggaata tcagcgagga aaaatagtga gcaatccaca gaggataatc agccgcccaa
     2041 agtgcattgc cagaatcttt taagccagtc cagaacgccg gcgatgtata aatgattggc
     2101 gttctgccag aaagctcttt tattctttct aaaaaaactt tagtaaacga tagcccaact
     2161 ccctgacatt ccaaatccaa agcaggggat aaggtaaatc cggtatcctt tatcaggttc
     2221 cagaatcggt ctgcctgctg caaagctgtt ccgtatatag tgcctgccgg tctggcatag
     2281 tgatacactc caaacggcaa ctcagcttgt ttgaatgtac gtgcgaatgt ttggaacgct
     2341 ttatcgttcg tagctccatc tgacgcgcgc aggaaggcaa actctacgcc tttctctttt
     2401 gcaataaccg gatcaaaaaa gtgttgcgga ttgcttgcat cgtttgtttg ccagtgtgaa
     2461 atgtcagttc ctaatatcat ctttcacctc tatattgtta ccagtcctgg gttctgtggc
     2521 tgccatagac cgtctttttt tcgccatccc caatcgaaag agcggtcgaa aaatatcatg
     2581 gtgcgtggaa caaaagctcc tggttcttca acggaaacga tcaaagctgg acgataatcc
     2641 tcggtagcat tatcaccgct gccaattctc acaagttcat tccttgtaag actaggacca
     2701 acggagttaa aatctctgct gcttcgcaaa ccataatagg tatatccatc cttatttatc
     2761 caatccgtat caaggtttcc actggtgtac tgggtatcta tagccattcc ggatgtattt
     2821 ctccaaatgg agttgtcttg attctcagaa aggattagat cgtaaacatt ttctcggttg
     2881 gtatcatcta ctggatcgaa agcggaccaa tcgcatttaa taatttgaat atcaaaatct
     2941 gttcccgtgt aaacattgac taaaatcgca accattctta gattaacttg taagatggtt
     3001 gccgtaggtg agataggaga tgtgtcaaac tttaaataag atcggtatac ataagatact
     3061 gcccctccag tttgagcctg tccaaccgta aagaactcgc cgccgaaata cgcgctggac
     3121 gttaaacgcg caatagcata agatgcgcta aacccataaa ttccaccatc accaggatca
     3181 gattcaaagt tcatatttta attctcctgg aacaccatga tcagcgttaa tccctttgtg
     3241 tcagtaccag ctgcatctac atcgcagcgg agtcgatcac cggtggcaac gtcatcatgg
     3301 tcggtatcaa tgaccggtgg agttgccgct gtgtagctgg tatactcgct ggcatcaatc
     3361 gtggccgggg ttgataatac atcctggtta tccgtggcat tgtgcagctg cactgtcgga
     3421 gcgccagaac tggagacggt atccactgcg acatcaaaat ccaccagctt atagccatcc
     3481 agatctgctg gaacaatgaa ataatccttg ccatcaccag ttgtcaatgc agcgtctttt
     3541 ccaattacgc gccactcaac gatcctttta acccacccat caagcttccc gccggcatcc
     3601 gctattggta tttttgatgc ggtcggggta gacgtggcgt tagcagggtc ttgtatcacc
     3661 ttgctcgagg cgtcgaggga cgccagacca ttggcagcag cttttcccaa gattgtcaag
     3721 acctctgcca gtgtcttctt tacccatgct cctaatccat ttcctacctg gaaatcgttc
     3781 tctgcggtag tctctggggc atttccccca cccaaagcgc ttacatctac gctcttatga
     3841 gcataatcat cttccgagtc agccagcaac aataaatctc ctgcaattgg ctcttctttt
     3901 tctggcgcat tttcaagatc gagcgcatca tccaacgcat cgatgccgtt ttctatgttg
     3961 ttcattctgg acgcgttgac cggcgatcca gcggcaatga cctctgttga aagctcgatc
     4021 tttacttctt cattgatagg agtatcatca gattgcagga tgttgtacag aggatcatct
     4081 actagttctt catctgtcca cgtgtttttg gtatatttct ttcccatcgt tactccatca
     4141 ttctgcaaat cccagaacaa ttccagttat gctttccatg tatccgtagt taggtgctgc
     4201 ggcagcatag gcttgaacct tacaaaccct gctacctacc gcaacactct gtttgaatcc
     4261 gataaccgta acagggtgac tccaactcgc actatggctt tccagcgaaa cctcattaag
     4321 ggttgtgtca tcaattgacc atcgcacaaa caggcgccca ctggtgccat acttgagacc
     4381 gtgaaatccc aatatggcaa ctagtgtact agtgcatgga accgtcaggt caatttttac
     4441 atctggtatg tccgcatatg ttgtggtagc aatatcggta ctcgatttat atacacttcc
     4501 aaaacacgat aatgacgatc cccacacagg cgctgttccc cctccattca tttttaagga
     4561 ctgtcctgct gttcctttcg ctaaacgtgc aagcgacgcg ggtccggaag cataaaataa
     4621 atcgccagct gcttgaccag ataccaatgc ttgtaatgct gcttgggcag caagatttac
     4681 taggttctga tatgaaatga ccttgatttt atttacttct agcggctcac tctggtcgta
     4741 gatcaggcat agatcaccag gtgccggcgt ggttaacacg gtatccggat tactctcaaa
     4801 atctgaaaat ttcttgctca tatttcctct cattcatgat cgtattccct gaacatgttt
     4861 tgtctggtca gatcagctcc gcatgtagct acgcctgacc tggcgcgtct ggctggatga
     4921 tcaaccggtt caatgtatgc tggctcaatt ccgagtagtt ccgtttgtgc caaatatcca
     4981 cctgccaaat ccgtgctcat cttttgcacg gtagccagga tattcttctg ataaagtgtt
     5041 ctcgttagta cgatatctcc caacgtgatt tcagatggta ataacgtaat tgtctttctg
     5101 taccgctgcc tgtaatagtc gcgcacgcgg tctagtatta tttgcgcatt atccatattc
     5161 acaagcgtgg cgctatccac caccaaagca tttttgttcg catattctgt gattcccgtt
     5221 tcacgaaaaa tatacgcacg cttgctatcc aaccagggat atccagttat ggttatgatc
     5281 ccactctgtg tcatttcaag gtaaaggaca tttggtccaa aaacatactc tccgcccact
     5341 tcgatttgat cgccggcttc agtaatgatg tattcttcat cttcagtaac tagataactt
     5401 ggatcatatc caggaccgtc aaccacaatg ttgtaatagg gtttttcaaa aatgatcttg
     5461 tgcgatccgg cttccaattc cttattaaat atttcctcca gctcactacc ttgtgtgtaa
     5521 ttatgtgaga ccagctctat actggtcacc agagtctgca gctcgatgga ttgctccatg
     5581 agtttgtctg tatctcgcac cctgtaatca aagtgctgcg taggtataac gaccggacga
     5641 atttgcagtc tgtcgcttct gacactgatg gcggcggcgc cggcagccag gcagatctgc
     5701 tgcaaagctt ctcggtagtt gcccggcgga atccagcccc tgattggcac gctttgaaga
     5761 gactcatcca gggtgtacag tacattaatt ggtacaaacg tttgcgccaa cactgttccg
     5821 agcggagtat ccgcgctcca aaaattgccg tcgtaatctg tggcatccaa tataccaatg
     5881 atgtctaccg cccggaactc aaactcgtgc tcgctggtgt ttttccactc tgataaatag
     5941 aactttccca aaaactgaag ttcaccatct acattctcgt atgccacaac gggcaagcgt
     6001 tcggacaata agctgaaata cgatccggaa aacatggaga acgattcatc gtagttcatg
     6061 attttcgctt ctagcgcgct gataggaagt tccatgccga tgatattggt ctcttcaacg
     6121 agcgtggcgc tggtaacctc ttcaccggaa aattccagtg tttcgcttcc cacagtcagg
     6181 cgaatgagcg gtttggtgat catgttctcg cagggctcct ggcgatgaaa ttaacggtca
     6241 accctttcca gtaattctgt atcccttgtg atttgcgcag cttgtctcct acattcgaaa
     6301 agtaagccgt gaacgtataa ttgcccgatt catcaggaac agttacagtg tgaaattcct
     6361 gcggctctgt cagttttgcc catagcgcgg catacaccgc tgtatctgta atagagccga
     6421 gctgcaagcg gtagttgaag tagacaccga tcagctcgcg gtgcagaacg ccatcaacag
     6481 tgcgctctgc aaatttatcc aaaaagtcag ccgtgcgttc tatgctgatg actggaatat
     6541 catactcaac accgtctatg atgatcacgc agcactcctt ctaaccaggc tattgccaat
     6601 gcgtgtattc tctttgtcga tgtatggctt gagttcgcgc accagcgccc ccaacgaccc
     6661 cgcaaatttt atctcgatat cggtctggat atttccaagt tcttcttgga tgatctggcg
     6721 tataagtcct tcaggggctt cgaggttgcg acctgatcgt tgatctccga gtattgcagc
     6781 gaattcggca ttgggcggga tgactgcacc agtggcaaga cgcggtatct ggggggcggt
     6841 tacggtcggg atatttaaac cgaaagaata cgccggcgta aggaatgttg ctggaatgtc
     6901 tattttgatg gtgttaagcg ctcggatcac tgcgttgatt ccggtggtta tccctcgaat
     6961 cattccgttg atgaaatcga tgattgaatt tatcgtgcct tttgcaaagt tcttgattcc
     7021 atcaaatgtt tcgtttatgc gtgcttttaa attagagacg ctttcaataa cgcgtagaac
     7081 tgcatttgct gcaccgattt gcaagtccgt ccacagagca ttccagtcaa actcgtcaaa
     7141 tcggcgcttt gtctcgtcca acaaattctt tatcggatta gaaccatctt ttgtaccaag
     7201 ttcccagagt agcttaatgg gattttcttc cagtcctagt gtttctaata gattctggat
     7261 aggattagtg ccaggctctg cgagcttcca gggaagcctt agcgggtttg atgcattaat
     7321 tcccagtaaa gttaaaaggt tttgaatagg atttgtaccc tcgctgatcc cccattccca
     7381 aaacaattta atgggatttt cttccagtcc tagtgtttct aatagattct ggataggatt
     7441 agtaccctct gcgaatggat ttttccatac atcggcaagc caaccactca ttacatcaat
     7501 tatgtcttcc ttgaaccact gcttaaaccc tatccacgaa tctttgataa aatcctcagg
     7561 aaccgcaact tctcgcatga taacatttcc acctgctcca cctcccgtat cggcttcatc
     7621 ggcggattcc atttgcagga cgttgatctc atcgaaggca gccaaagcac cctttgcagc
     7681 cttctccgtg tcttttgtgg cttttgccag tttggcagtc gatccggcag cagaatccac
     7741 cgcacctgac acatactgca taactgtctt ttgaccggtt agtgcagcta caaacatggt
     7801 tacccagttg atagccttta ccagccaatc gataattttc aacaagactg gggcaagagc
     7861 atttaataaa gtcgcgccag tagccataac cgcgcctttt aatccatcaa acgcaccttt
     7921 gagctgaacg acccgatcgc gcatggcagt ggtaacggac aggtttttat gcagcgtttc
     7981 ggttagttct tgcgaccatt tgccaaccac agcaattaat ccaacaaacg cgacagaaag
     8041 aactacaatg ccaattgcgg cagtttttag taatgacaat attgcggtcg caatattttt
     8101 aagtgtgccg cccaaccgtt tgacaccttc attgaatccc ttttcatcaa cgcgcgtatc
     8161 tattctgatc gttccatcat atcctgctgc caaaatacac ctcgtataat aaaatctgta
     8221 tcataattaa caaatcatcg tttcgtacat aaggagaaaa catgtccaaa acaaccactg
     8281 ctggtgctaa aactgaagtg cgagttgaaa tctacaaaaa taagcacgac ctgaaaaaag
     8341 gaatgaaaaa agaacaggat cttggctgga cggttctttc cgttcaaaat gttcctcaag
     8401 gatatggttg tttcaagacc ggctgtctcg gtttggtctt tttgcctctc gcgcttctgg
     8461 ggcgtaagcc ggaaaagtat caggtcacat atcaaaggca aatagcacaa aatcctaatc
     8521 attaattgcc tcttcgggat actcaatatc ctgtttgtgt tgtttctttc ctgttgctac
     8581 cagttccata aagtgtgctt ctgcttcgcg ttcttctagt gtgcggcgat caatatctgg
     8641 caagtcaaag atttccttta tttccctggc gactgcgcgt tcttctttgg ttgctttgcc
     8701 agtttttaca cgctttctca aactgaccaa actgctgaag gtagtttcgc tgcctaaatc
     8761 catgaagaga gccagaaatt tccaccaatg catctcaacc gcgtcaagat caataccgtg
     8821 cgtctgtttg aatgctgcca tgatgtacga cgcatcctgc tcaaacgaat acaaacgcag
     8881 ttccgattca ccattgcttt cgccattgcc gctatccttg ccgccgtcaa gaaacctgat
     8941 acctttttta atcgcctgcg ggatatcgtg tggcatggac ttgtacaggt tatttagcaa
     9001 gatcattcgt ttttcagcat ccgccaattc attatcttca aacgccagaa taatccgcag
     9061 gcaggagcga aagcctgaat tgatctcata ttcaattcca tcaatttcga tagcaagggg
     9121 aagactttca gtcagaatgt tcacttcata gaccgccttt tatccgtaac agaattcgaa
     9181 tactttttca ctctctcatt cctggcatcc cagataaatg gagtaattcc ctcaaaaaag
     9241 tctgtaatca tatccacact gagcgcatct ccgaaaactt tctgacttgt tccattacca
     9301 aacagatcat ctatttttct acggacatat gtgcagaatt ctttctgcaa tgcgaaatta
     9361 tcattcgcat cagtcaacat accattttca tcaagaccga cctcattcaa tttcgcatat
     9421 cgctgatcga attcttccag cctgacctga aactcctgac acattgcgta gaaacgctca
     9481 acaaaaagaa cgtctgttgg attgaactct atgacattgt ttggatcgtc gttgatagca
     9541 atccgtttaa tgccagatat tttcagagta ttcatattgc tcattagctg ctgccttcgc
     9601 tttcagacgt gaaggtggcg tcagttggat caaacatacc ctgcactgca tctccctgga
     9661 aaaggaatgt gaaattcagt ggattggcgc ctggctctcc agagaattcg tctatctgaa
     9721 cctgtacggt ctgtttttcg gcaggatact caccgccgac cggtgtttcg tacagataca
     9781 ccataatgat gtcggtttca gcatcgctta gagttgctcg ctctctgcgc agcccatcaa
     9841 caaacatgca aacctcatca tcggtgtcca ttttcatctc tattggcatg gtcggacgat
     9901 atccgactat ggatgttgtg cccgattttt gatggatata ctgctcttcc tcggtttgcg
     9961 gctggtaatt cacaggcgcc gaggtgatac cctgcccaac cagagcataa tccggcatag
    10021 attgggttcc aacatttaaa aatgtgacaa actgggttcg gtctatcctt gccattttct
    10081 acctctttct tatgaacttt cttctgattc ttcttcgcgc ggcttttgtg tgtatacaag
    10141 ccggcattgc acttgataaa ctccggtctg gctgtcgcct tgttcataca agtagcccca
    10201 accggtcgct tcgatgctct cggcaatctg cctttctccc aacactggcg ggttgccgct
    10261 ctcagactgc aactccagcc attccgcaaa tgcttcataa aatccctgcg tttccagcct
    10321 ctctagatca tccgctgtgc tttccatcgt ctggaaggca aacgggtagc tcatgatctt
    10381 gtttccgaca atatcttctt ccagcacgcg cgtaccaggt aagggcgtga tggcgtactg
    10441 cgtaggttct ggacctaaaa aatctaccca aaccggcgca ccatgtttca agctggtata
    10501 ggttttgata tagtcctgga ttgcgctgat aatactcact tgccacctcc ggctatcctt
    10561 cgagcgcctt caataatgcg ccgcccatga acctctttca tacgttggaa ccagtatgga
    10621 ccgcggagcg gacccgtttg actacctggt ttgcgccggc tgtaatattg ccggcgagcg
    10681 tatggtgcaa tatatttcac ttcaccacta ccgatctctg ttcctaaaat tccagatttg
    10741 atcagcatac cggttctcaa tggaacatat ggctcacaca accgcagtac ttcggaatct
    10801 acaaactttt gcgcctccga atagcgtttc tgccatttcg gctgaaagtt ggtattccac
    10861 accaattcag cctttccatt tttgttgata aatatccttc cacgcggggt cttaataacg
    10921 ggtccttttg ccattaacta cctcccacct ggatatgctg catatgcgat gatccgtagt
    10981 ccatcgtatc cacactccgg acctgaatga cctcgtaatc agcccttaga ttgctggtgg
    11041 tgtaactggg tgaaataact ttatccgccg tccctttgac cagcatatcg ccaatcttga
    11101 tggcaatgct gatgtcaggg atatacacag cggctttatc ggcctccagc agcccggact
    11161 tgatcacatt agccgcctta cgttgctccc aaaacacggc agggataaca gagcgcgccc
    11221 agacttccga accgcttacg actgaccttg aataaagcgt tgcggcggta ttagttcgca
    11281 tttgctgctc ctccatactc accagccgca aaaccagcaa acatcagacc cgtgctgccc
    11341 aaatgcaatt ttgccgcgtt cgccaggcgc tgatcgctgg tcaattccat gtctttggta
    11401 acgatatagt tctgtgagaa gtttccaacg cgttcactag ccacttcacg cggctggcta
    11461 atctgtctct gcatttcatc tgctaccgca caggtggcaa gcttgatctt ggtgatggtt
    11521 tcggtgtcct ctgccgcctc ataaattgca gccgcgcggt cgaaggtgat ctggtctatc
    11581 ttcgcgctgg cacgcaaagc caatgctgga aacgcagctt gtgctatggc tgttccgaga
    11641 taagtagtgc tgtaaaattc ccaatttgcg taagcggtca tagtaccttc tccagaaagt
    11701 gtgctttgga acgcttcata ttttccttgt atccctcaac agtcattctt cgatgaaaat
    11761 taccaggttg atcagctagg tgaacgaatg gcaaatctaa aatttttaat gggacgccct
    11821 tctcttggca acgccaggaa taatcaagat cgtcaaagcc ggcgtgcaga taatattcgt
    11881 caaactcgcc cacaatctcc cacacggcgc ggtgcatgat cagcagccag ccatagacgt
    11941 agcaaaaatc ttttcctgca ccccacttgg caggtttctc cctgacctcc atgccataca
    12001 gccagctaaa agattggctc tcaattgttt ttgcaaacgc acccttgcac aggacatcat
    12061 cattggaaaa cattagccaa tcgccgtgtg ccgcacgtgc accagcgttg agtgccgcca
    12121 tatagttata gtgttcagtc ggatcaatcc ggatcaggtg gtaatcacaa ctggacggat
    12181 aaggctccgg agaaccattg tccaccagga tgatttcggt attcggctca tacctcaata
    12241 ccgattcgat atagggagtc gcaaagtcgc cccatttgtt attagccacg ataatcaagc
    12301 tgatcattgc acctcccctg cccacagcgt ccgatgaact ccgatgcacc gtaatttatt
    12361 attggggatt gaatccacga agcgtgtaac cccctcccat gcctcgctgc cgtaatcgtg
    12421 gaaagcgatc aagccgcctt tgacaacgag gggaaaccat agttcaaagt cctgtttgac
    12481 atgcgcatat gagtgattag catcaatgaa cagtagatca atggatttat cccattccgc
    12541 ggcggcttct gcggatgact tccttatcgg cacaacataa tcaagcagtc caagtttctt
    12601 gagattcgcc ttataatctg ccagccattt ttcatcatcc caaatatcta cgctgtaaat
    12661 agtgcctttg gtcctggcat agcgtagacc ggacgccatg aacgccgcag atctgcccgt
    12721 tgccgcgcca atctctacga tggtttctct atcatgcgca agttttgcca gccggcagcc
    12781 ctcgatggtt ttgacgccca cacgaccgcc aggaatggct ttgttgatat ttttgagatt
    12841 tggcaggtcg ataacgcgtt tagtgaagtt cacgctcata aataactctc caaagtcttt
    12901 acaaacgact gtccattgaa cggttcgcca atgaagcgct gtttccaatt atcaataccc
    12961 tgatcaccgc ccattgccag tttcaacatg tcgtacaaat cctccgatgt ctggcagtcc
    13021 tcaacgttaa acgggtaagc catgtaatcc ctatatttct cccagttctt tgcccataac
    13081 aactggccgc cagagcgtgg actgttgtgg ggtctcacac cctcgccgag cattaccagt
    13141 ggatgaccta acgccactgc catgtaagcg tatgtgtatg ccccaatcac aacgtcagcg
    13201 ttatccatgt cgtccgttga accatccatt tgcccagcga taaacttaac gcgctgatca
    13261 ggctcaatgt tgttgtattt cagcgaaccg atatgccgca cagtgagatc aattctgtcc
    13321 aacagtccga gtagcaattt ataggttttt acgttcagct cacgatcaat atccggcaaa
    13381 aacgaaccgc ctactggatg gagcggggca aacagcacgc gcactttttc aatcgagcta
    13441 ttacacctga acgaacaaat ctctgtatat ggccaaccga ccacctcgat tggatttgga
    13501 taccccaatt ttttcagtac cactttatga ccttctgcta tagtgaacaa agcacgaata
    13561 ttcgggtgaa attccctggt caagtcaaac ggtatgttgg gacggacact gtgtggataa
    13621 acaaaaacag gtatgcccaa ctgctcacac tcggcaattt gactgcggaa tttagttggt
    13681 atatagccgg cgaatagccc gtcccactca tggtcaatca taacaaaacc gataccctcc
    13741 gctattctgt gcttaactcg ataccctgcg tgcatcagcg cgtcaacgta tggtttggct
    13801 ttgtgctggt gctcgtaaac gtagaatcgc atttacttac gatttggcag atgtcttgcg
    13861 tgcaacttct ttttcatgaa gcgcttggta tgacttataa aataccaact caagcagatc
    13921 atgaagagcg ccagcgccgc gactgatgag gataccggta aaaatactgc ctagtggttc
    13981 ccacagtgaa ttgaacgcag ccagtttgaa gatgtcaatt ttgaagaaaa cgcaggcgaa
    14041 caccgataca gccagactta ccagcacgtc gggattaggt ctgaaggttt tgttgtcgta
    14101 gaccaatttc aggttctgaa cgaccgcctc aatcaaaccg gcaaagatca acaccttacc
    14161 aaacaaatca acaaaatcag cgagattttc aaatttcatg tttactccaa tgaggggggc
    14221 gattcagccc ccctcttgaa tttaaaattc tgcttaactt gaagccccat tagccacaat
    14281 ttgaacgccg cgcaaaacgc cagcggattt cgtggctttg acggccacgg ccgcaaccat
    14341 ctcaacttca cccaacttca ccgcaccagg taccgtcatg tcgggtagat agatctgaac
    14401 aaggggagat ccttcgggtg atacgccatg aacgccgtcc agcgcgatcc ttgcgcagta
    14461 aatactggtt tctccgtatg catccacccc gatgatcggg ttcgcagtgc caggtttgtc
    14521 gcccagtgcc atgaccagcg acggacccca aacagcaact tcatcgccgt agttttcttt
    14581 attggcaaga ttaattccgg cgcgatccat gactgactga aatatgccaa acatatcagt
    14641 attcatcatg tacaagctag gtgcaccatc tagcttggcg cgcaatttcc gcaggtggtc
    14701 taaaaagacc ttccaattgg tgtcaatgtt tgacgatgtt gaaagatcaa tcgttgtggt
    14761 cggctcaagc tcagtcgaac tgcctgtaac cgccttgtta ataccatcaa actgggtggg
    14821 ggtagctgaa ttgccattaa tgaaccaatc ggcaaacaac gcgcgggttg ccttgatctt
    14881 ctgctcgatc tgaaattgga tgtgattgac gacctgtgtt tcatggttaa taatcacgcg
    14941 atcaagattg aagctaccgc caaaaggctt tagatcgacc gtgtagcgcg tggttttggt
    15001 ctcctgagcg ccatattcac tgttgatcgc tcgtccagca gcggtcgctt gcgtggtgat
    15061 gcgattgtac gcataggtta gggattcacc gccctgcggc ttgaccgtgt catcgaagat
    15121 catcacgtca agcaaggcgg attttctaaa ctcatcaata acaatattgg tgagtttatc
    15181 ctgtgaaagt tgttttgcct ctgctaatgt aagagccata acttacctca tttctttttg
    15241 gacagccttt cttggatagc ctctgcaaag gatcctgtag gcgcgtccgt agtttgtttc
    15301 ccgcccgtta tgatcttcgg atcgggggtg tccgattcga acagataatc gttttcggat
    15361 ttgatttttt ctaactgttc attcagccca acgattgagc catcatcagc cagcttcaga
    15421 tcgtctgttt tcagcagtgc cttgaccgcc ttcgcgttct tcgctttcgc ggttgtaagc
    15481 gcaccatcca gggcatgatc aaacttcaac cccgcgatgg tcttgtcagc ttctgcctta
    15541 gctacctcgg ctttcgcttt ccaatcgtca gccgcttttt gcagctcttc cggcttcata
    15601 gccttgaagc tctcgatagt cttattggct tcctcgagct gcttcttcgc gccatccaat
    15661 tcggactgag ctgctgacag gtcggttttg tgcttttcga tgtctttgcc gtgcaggata
    15721 ataatctgat ccaaaacatc agatccaaga cctgcttttt cgattgcttc ggctgtcaag
    15781 ccgagttttt ccaaatcttt cttgttcatt atctgttccc tttcatttca ccttatcttt
    15841 tttgagttgg tctgttccaa ccggatgccc acatttttac gtttgtggtt aacgaaaaac
    15901 ccgaagtgct ctaaaagcca cttcgggatc atgcctcgtc a
//
LOCUS       DAOSDS010000046        15871 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1491_length_15871_cov_5.412620, whole genome shotgun sequence.
ACCESSION   DAOSDS010000046 DAOSDS010000000
VERSION     DAOSDS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15871
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1491_length_15871_cov_5.412620"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PLL88_06735"
     CDS             complement(<1..930)
                     /locus_tag="PLL88_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOJ01301.1"
                     /translation="MWSQDAIVYQIFPDRFYNGNQTNDPDGTVNWQAKPTVDNFFGGD
                     LEGILVKIDHLLDLGINTLYLNPIFKASTNHRYDTVDFYQIDPALGDTDFFRQFVQEC
                     HQRDIRIILDGVFNHCGSDHPFFQDVIKNGPNSPYADWFCFQGFPISSDPLNYKTCGG
                     CSYLPKFNLENLAVQCYLLEAARYWTSDYQIDGWRLDSAQRVPHHFWNTFQREVRKIN
                     PQIYLCAELWHEPTGWFQQGLFDGASNYLLRQLLLDYFVEETIDAEDFLYELTSLYNR
                     LGENANTMLNLVGCHDTPRINSLFAHSPEALSAL"
     gene            complement(941..2071)
                     /locus_tag="PLL88_06740"
     CDS             complement(941..2071)
                     /locus_tag="PLL88_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HOJ01302.1"
                     /translation="MNSIFYEKSIAVILENQHEHGGYIACPNFKQYQYYWFRDGSFIA
                     YAMDIAGQLNSSQRFHQWAVQTILRNKAKIMAAAEKVKGGEMASITDFLHCRFNANGS
                     DAEGDWAYHQLDGIGSWLWSFSQHFRLSGRTHLTAPEDEAISLACLYLQALWNTPCYD
                     CWEENGNAIHTYTLAAIYGGFHEINKIKPLENGIASSQQIQEFIFQNQFQDGVFRKSS
                     KDHGVDANLLGLVFPFKLIEPNSTLFEKTLQAIEVDLLTSQYGLHRYSDDSYYGGGEW
                     ILLSAWLGCVYMIQHKVAQAIEILAWVESQITPKGYLPEQSGAGFSNARCFHFWMKKW
                     GTIASPLLWSHAMYIILYQSIKQYHDTMGLRLENKPHIINRR"
     gene            complement(2075..2695)
                     /locus_tag="PLL88_06745"
     CDS             complement(2075..2695)
                     /locus_tag="PLL88_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ01303.1"
                     /translation="MNSVFVTLLTTFSVVLMSSMMAFAFSRFTFKRKKLLSGLIRLFM
                     MMPSMTLIVPQFILASKMNLLNNLWGVIMVYIAQNLSLSVFLLTGFFKQIPVEIEEAA
                     KIDGASPWQVFARIILPLSTPALATSAIFTSLGAWDEYVWAMTILNKPEMRTLPVGIA
                     SFHGQFVTDWGLVFAASVIAITPIIILFIFFQKYFVKGITAGSVKG"
     gene            complement(2928..3812)
                     /locus_tag="PLL88_06750"
     CDS             complement(2928..3812)
                     /locus_tag="PLL88_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ01304.1"
                     /translation="MKNKKHLSAPYLFLFPGFVFLTLWLIYPMFKALQISFYDWNFLA
                     TIKNTFIGFDNYIRAFKDTIFLLSLKNTIVYAIVTVSGQLILGLSVALAIDRITCGKI
                     FFRTAYYLPVVTSWVVCSLLFKFLFNASPAGLVNYALIDILHIIENGIPWLNEAGTAF
                     LALFTLGIWKGVGWTMVIFLAALQSISKEVLEAAQIDGANEWQIIWYVIIPMLLPTIF
                     LVIVMLTIGAFQTYIPINLITNGGPVHRTEVLLSYMYDQAFKNWDFGYASALSYILII
                     LVFSISQIQIKLSNQIEK"
     gene            complement(3869..5170)
                     /locus_tag="PLL88_06755"
     CDS             complement(3869..5170)
                     /locus_tag="PLL88_06755"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ01305.1"
                     /translation="MMKKPFMILTICSIFFSLVISGCTQAKTGEETAQVETIAEKSPA
                     EEVITLSFWHTYSENSPENDTLVNTLIPIFEEEHPNIKVETLIVPWEDFRSKLYTSIA
                     GGVAPDLARIDIIWVPEFAEMGALVALDENIEDFQTIADQVFSGPLSTNAWNGHYYGL
                     PLDTNTKAWFYNKAMYEAADILPPTSFDEMLEQCEAIKAVYPDSYYFATDGTFAWVTL
                     PWIWSFGGSITDDAITTATGYLNGPGTVAAYESLLQLYNNGCIAPVILGSGIDVFTGY
                     AQDNYASLDNGPWTFSIIESQFPDKEVSVSAFPAGPGGSINVVGGEDVVLFEKSKNKE
                     AALEFMRFLLSEEYQLKMAEVGQIPVLKDAVNSDYMQNHPYYTVFLEQLTTSKSRTSH
                     PKWEQMDQIITDAGQLILRGEKSVQEALDEAAAEIDILLQE"
     gene            complement(5283..6302)
                     /locus_tag="PLL88_06760"
     CDS             complement(5283..6302)
                     /locus_tag="PLL88_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ01306.1"
                     /translation="MATIKDIAQKAGFSIGTISNYFNNPDLLNDQTYASIKQVVEQLN
                     YHPHYAARALKSRQTHKIGLIPVISVEENKQQVSSDYVFFDFLSAVNTAAAERGYGVL
                     LETVLVENDETEKYVQLIGEKQIDGFVLLGTQVHDQRVNLLLDLNFPFVTFGRTSRQN
                     EHAWVDVDGAKAISKAVEYLASIGHKRIGLITPPSGLFCSKERLESFHLCMRDLNLEI
                     DDSMIIQGGFSEESGIQVMNTLLNSSQVPTALIAPNDICAFGLMKAMQMNSLKPGRDI
                     AIVGFDNIPLAYYWQPSLTTIAQPIKKIGDMVTNILLDVLEGKSAPVHKLIEPELFVR
                     ESTSA"
     gene            6453..8450
                     /locus_tag="PLL88_06765"
     CDS             6453..8450
                     /locus_tag="PLL88_06765"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ01307.1"
                     /translation="MKNKIFLTVIILALGITACVNKPQASTGSSNLPETALAIEGPVL
                     LTGSFDYTNEFVLETYYVEHAVMLTDLTSFILRDLEWELPVESQVLGYLDVDEDANRA
                     TYRLALPVLPEGEMQDVDQDGRQDSGVQVFAVAYSPNLTGDTFSVGDDRTWGWPSYLT
                     SVKTDTENDDEIIGGKLVIWAPDDSQQFPSGFGADGLLFTADDPVMDVPAGYSVIDLD
                     TDPFEIYRDRIVDVELYEPQDVAIKDYSDLSYSEAFQTMFDTISVEYAFNGVEGKEPD
                     WQALYDTVMPMVEQAQRDRDPHAFFQALHMFTLGFDDGHVYFDGNDYYWNYLYEQVFP
                     GFGFSAKETDAGEVVVVYVTADSEAERAGMQVGAQVLSVNGAEVEDWIAQSIPFFPTS
                     SAHLYRYDQVTSAFRAPEGEQMTITYRNPDGRNRQVTLTSFYDPYSFINVDSGWAWYF
                     ASVMPVEFDLLSDDIGYIKISSNSDDIGLIIRLFQHALQTFTDYGATNLIIDMRVNGG
                     GTNLGLAGFLYDQNIPIGQLEYYSEKTGRFEAEGLMDEVYPNEKQFRFEQIILLVDQY
                     CASACELEAYGFSQVPGVVVMGENPTAGMEAEVARGEFVLPENITLTIPTGRFIKQDG
                     SILLEGVGVVPQVTIPTTIESLTADSDMTLQAAIQYIYGNY"
     gene            complement(8500..9237)
                     /locus_tag="PLL88_06770"
     CDS             complement(8500..9237)
                     /locus_tag="PLL88_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01308.1"
                     /translation="MKTKHPVLKKVSWSFWYGVGMALLEAVVVIYLRELYYPQGFRFP
                     LAPMDWNIYKIEVLREVGTLLMLVCVSALAAENFTTWLAYFLFNFAMWDIFYYGWLKV
                     LLNWPASLMEWDILFLIPITWDGPVLSPLLCTVAMLVLAGVILFHHQRGASVKINAGE
                     WALLLGGALLVFISFIWNYTSFMLQGGYLKDLATLGSNIAFQQASAAFVPPGFQWGIF
                     FSGYGLIWSAIVLLARRSRQHSNGPQK"
     gene            9353..9982
                     /locus_tag="PLL88_06775"
     CDS             9353..9982
                     /locus_tag="PLL88_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01309.1"
                     /translation="MKDKIGQIMRKTYSYWWVDGMAEMGIGLFFGILAAYNYLIETLP
                     LSATASLFMAIAQPLVFLAGWFGYGRLVRWVKERFTYRRTGYVAYQPKNRKNRAVRAL
                     VGFVMGFGMALTVTFIGPEVLEIDSLIMVGGLLALVTLFLAIWHGVNRLFVVAVAEFV
                     LGLWSSRLALEADLRSILLMACIGGVWFLSGLITFALYLARTKPGQEEA"
     gene            9979..10296
                     /locus_tag="PLL88_06780"
     CDS             9979..10296
                     /locus_tag="PLL88_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOJ01310.1"
                     /translation="MNDGALRDLAEVDRLIHEPARLVICTILYTVKEADFLFLLNESG
                     LTKGNLSSHLTKLEEAGYIDIRKIFNEKKPQTLCSMTPAGRSAFEEYRKYFKKIIERM
                     NGE"
     gene            10297..11235
                     /locus_tag="PLL88_06785"
     CDS             10297..11235
                     /locus_tag="PLL88_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01311.1"
                     /translation="MTNENISISTHDLRCQFGEVRAVDGITFEVQTGTLFGFLGPNGA
                     GKTTTIRLLLGLLEPDGGSAQVLGYDVRTQADQIRLNCGALLEQSGLYERLSAEDNLD
                     LFGRIWHIPTEQRAQRIKELLEKMGLYERRKETVNTWSRGMKQKLAVVRTLMHNPRLV
                     FLDEPTAGLDPVAAASLRDDLVDLARQHSVTVFLTTHNLAEAEKICDRVAVINKGKLL
                     TVGSPDELRLQKSTHSLTVVGRGFSPEVVAALRAMPEVEAVELQDRHLVVHFKQETDS
                     GNLVQFLAGKGVMIDEVRKGKASLEEAFLALLEEKE"
     gene            11232..11975
                     /locus_tag="PLL88_06790"
     CDS             11232..11975
                     /locus_tag="PLL88_06790"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ01312.1"
                     /translation="MIQDILILIRKEWKEVIFQRAGGRQSWISILILLALLGVYLPYM
                     NGVDWLYNPTALFAWAWVPLFMTIGLVVDAFAGERERHTLETLLATRLSDTSILLGKI
                     AASVLYAWSISVICALLGAITINVLFPSGTFQFYNAGMFFGGIAAVLLIAILISSIGV
                     FVSLKAPTVRQAHQKLSFSIMVVWFVPFILLQAFPDKVQDFFVSIAPLLENNLPLIIG
                     GLFLLLVVADAAVLLLAKKRFQRTRLILE"
     gene            12089..13186
                     /locus_tag="PLL88_06795"
     CDS             12089..13186
                     /locus_tag="PLL88_06795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ01313.1"
                     /translation="MKIAYVTLHWPRFNTGGVSNKILHQIHLWESQGNSIIAYMHLHT
                     VENPQDLLPARYFFYSRKKFPLLIPYQEISRSIALLKLINALKKDQPDVIYLRWGMYT
                     FPLQFLPKNMPIIIEINTNDLHQHQLLGRILSTYNKITRSITLSLADGMVFITNELAD
                     DPAFARFTPQRVVISNGIDLDEYPFFKAPKTDIPHLAFIGTPGLPWQGVEKLVDLARL
                     LPDIHVDIIGYDTIPEVDKMPSNLTLHGYLNQDAYRQVLVKATAAIGTLSLYKKEMQE
                     AAPLKIRECAAYGIPLILPYKDTDLHGQKCDAILELPNTEDNITENAQRIHDFLYRMQ
                     GRRLERKIIYPLLDIKQKELARLNFFRSFIK"
     gene            complement(13283..13780)
                     /locus_tag="PLL88_06800"
     CDS             complement(13283..13780)
                     /locus_tag="PLL88_06800"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ01314.1"
                     /translation="MDIGCGKNNIFIKQFLNNHGIGLDIFPYEGLTEDNLVETFCELP
                     YPDNSFGTVTFIANFNHIPRDLRSQEIREAYRIVERGGRIIVTMGSPIAELLAHLNVW
                     LQDKFFGTNIDMDSERGMAEGESFFVLDEEIKGCLENSGFSNLERHYFPTQWWLNHLW
                     IGFKG"
     gene            14182..>15871
                     /locus_tag="PLL88_06805"
     CDS             14182..>15871
                     /locus_tag="PLL88_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01315.1"
                     /translation="MLKEYLSQKSTERGILTVAGVLIAACFTHHVFSIWDRKALWILL
                     AFLLLSPLFTLVLSFIYAQSKSAFQQIQRKRSLFLISALVLGSLTTWATYRAPVSYQT
                     ITLEPQLYAHQRIELLEIKVGGDIYSFEEKALEAGWNLEDGMFTATQDSDPLILTFPV
                     PLRSKVNILFNSSVQSGRVSISYGSMRRAVDLSTSNPQQKLITLHSQYRTVPNWLFVP
                     FLVIADVFTFSFLFLLIFILQEKGQRKLIEDHRPEKFFSHQQCVLILVSVAVILHLLN
                     ALAVPLIVDSDSPAYLQGAVHLLQYGNLKGVSVFCGPGTTFLFAPVLYVFGRNPWGMK
                     ILLHLIAIASVLVSYRIGWQLSHKRWVAFCIGFLTILIPEFYYYSNFVMSEIPNIFLI
                     LLSSSLFLDALVDFSFQRILLAFGVVSLATLLRAENILVFLIGAVCLGSKPAWDWFNG
                     FFDKTKKQQKHSSHPPLAMLFWAALIALIPVIWLSLFNYQAHGFLGLNSSSGVVLYGG
                     WVYYPEASGFDFRDESSPAVQEIDRWIEKYPIDITDGSGIATPGEIYPSLIKAGF"
BASE COUNT         4098 a         3688 c         3829 g         4256 t
ORIGIN      
        1 taatgcggat aatgcttcag ggctatgggc aaaaagactg ttgattctcg gtgtgtcatg
       61 gcatccgacg aggttgagca tagtattggc gttctcgccc agccgattgt ataaggaagt
      121 caattcatat aagaagtctt ccgcatcgat tgtttcctcg acaaaataat ccagtaacaa
      181 ttggcgcaat aaatagtttg atgctccgtc aaataatcct tgttgaaacc agccagtcgg
      241 ttcgtgccat agttcggcgc aaaggtatat ttgggggttt atttttcgaa cctctctctg
      301 aaatgtgttc caaaaatgat ggggaactct ttgtgcgcta tcaagtctcc aaccatcgat
      361 ttgataatcg ctagtccagt aacgcgctgc ttccaataaa tagcattgca ctgccaaatt
      421 ctcaagattg aacttcggta aataggagca tccgccgcag gttttgtaat tcaatggatc
      481 gcttgagatg ggaaagcctt gaaaacagaa ccaatctgcg taaggtgaat tgggaccatt
      541 tttgatgaca tcttgaaaaa agggatggtc gcttccacaa tgattgaata ccccatccag
      601 tatgattcgt atatcacgct ggtggcattc ttggacaaat tgacgaaaga aatcggtatc
      661 tccgagtgcg gggtcgatct gatagaaatc tactgtatcg taacggtgat tggtggatgc
      721 cttaaaaatt ggattcagat agagggtatt gatgcccaaa tcaagtaaat gatcgatttt
      781 tactaaaata ccttcgagat ccccgccaaa aaaattgtca acggtcggct tggcttgcca
      841 gttcacggtg ccgtcagggt cgttggtttg attgccattg tagaagcgat ctgggaaaat
      901 ttgatagacg attgcatctt gtgaccacat gattttatcc ttaccggcgg ttaataatat
      961 gaggcttatt ttcaaggcgt aaacccattg tatcgtgata ctgtttgatg gattggtaaa
     1021 ggatgatgta catcgcatgc gaccataaaa gaggcgatgc aattgttccc cattttttca
     1081 tccaaaaatg gaaacagcgc gcattggaaa aacccgcacc gctttgttcg ggcaaatatc
     1141 cttttggtgt aatttgcgat tcgacccatg ccaatatttc aatggcttga gctactttgt
     1201 gctgaatcat ataaacacat cccaaccagg cgctcaataa aatccactca ccaccaccat
     1261 aataagaatc atcggaataa cgatgcaaac catattgcga tgtcaataaa tcaacctcga
     1321 ttgcctgcaa ggttttttcg aaaagcgtgg agtttggttc gatgagctta aaagggaaaa
     1381 ccagtcctaa taaattcgcg tcaactccat gatcttttga tgattttctg aagactccat
     1441 cttgaaactg attctgaaaa atgaactctt gaatctgctg actggaagca atcccattct
     1501 caaggggttt gattttattg atttcatgga atcctccata tattgctgcc agtgtgtatg
     1561 tgtggattgc gtttccattc tcttcccagc aatcatagca gggtgtgttc cataatgctt
     1621 gaaggtaaag acatgccaga ctgatcgcct catcttctgg ggcggtcaaa tgcgttcttc
     1681 cggaaagtct gaaatgttgg ctgaatgacc ataaccatga gccgatgcca tccagttgat
     1741 gataagccca gtctccttct gcatcgcttc cgtttgcgtt gaaacggcag tggagaaaat
     1801 cggtgatgga cgccatctct ccacctttta ctttttccgc tgcggccatg attttggctt
     1861 tgttgcgcag gatcgtttga actgcccatt gatgaaatcg ctgcgagctg ttcagttgcc
     1921 ctgcgatatc catggcatag gcgatgaatg aaccatcccg aaaccaataa tactggtatt
     1981 gtttgaagtt agggcaggca atgtaaccgc catgctcgtg ttgattttca agtatgacgg
     2041 cgatgctttt ttcatagaat atagaattca tggattaacc cttgacggat ccggcagtaa
     2101 tccctttcac gaaatatttt tgaaagaaaa tgaataaaat gatgatcggc gttatcgcaa
     2161 tgacggatgc tgcaaaaacc aatccccagt cggttacgaa ttgcccatga aaagaagcaa
     2221 tcccgactgg tagagtgcgc atctcaggtt tgtttaaaat ggtcatagcc catacatatt
     2281 catcccaggc gcctagagag gtgaagattg ctgacgttgc cagcgcagga gtggaaaggg
     2341 gcaatatgat tcgtgcaaat acctgccatg gagaagcccc gtcaatcttg gctgcctctt
     2401 cgatctcaac aggaatttgt ttgaaaaaac cggtcaggag gaaaacgctt aacgatagat
     2461 tttgggctat ataaaccatg atcactcccc acaagttgtt taacagattc atcttgcttg
     2521 ccagtatgaa ttgtgggacg atcagcgtca tggaaggcat catcataaaa agacgaataa
     2581 gaccggatag caactttttt cgtttgaacg tgaaacggga aaaagcgaat gccatcatgg
     2641 aactcataag gaccacagag aaggttgtca gaagtgtaac aaagacgcta ttcataaaat
     2701 atctgtcgaa ttgccgtgtt gtgagtacgg tcagatagtt ctgtagtgtg ggatttgagg
     2761 ggataaattt tggaggaaat tcaaaaacga aaatattttc ttttagtgat gtggaaagca
     2821 tgtaaaggaa tggagtgact gtaaagaaaa gaccgattaa tagaagaaaa taaagaaaca
     2881 ttttctttcc gattgttttt attggctggt tcatattctt tctcatttta tttttcaatt
     2941 tggttgctta gttttatttg tatttgactg atcgaaaaga caaggatgat gaggatatat
     3001 gaaagcgctg atgcataacc gaagtcccaa tttttaaatg cctgatcgta catataactt
     3061 agtagcactt cggtacgatg aactggaccg ccattggtga tcagattgat agggatatat
     3121 gtttgaaatg ccccaatggt cagcatgacg ataacgagaa aaattgtggg aaggagcatt
     3181 gggatgatga cgtaccaaat aatttgccat tcattggctc catcaatttg tgccgcttca
     3241 agaacctctt ttgagatgga ttgtagcgca gccaaaaaaa tgaccatcgt ccagccgacc
     3301 cccttccaga tccccagggt gaacagtgcc aaaaatgcgg tccctgcctc gtttaaccag
     3361 gggattccgt tttcaatgat atgcagaatg tctatcaggg cataattgac caaccccgct
     3421 ggacttgcat tgaataaaaa tttaaacagc agggagcaaa caacccaaga tgtcacaacc
     3481 ggcaaataat aggctgttcg gaaaaagatc ttgccacagg taatgcgatc aatcgctaat
     3541 gccacggata atcccaaaat taactgaccg cttaccgtaa caatcgcgta cacaatcgtg
     3601 ttctttaatg acaggaggaa aatagtatct ttgaatgccc tgatgtaatt atcaaaacca
     3661 ataaacgtgt tctttatggt ggcaagaaag ttccaatcgt aaaaacttat ttgtagcgct
     3721 ttgaacatcg ggtagatcaa ccaaagggtt aggaatacga acccgggaaa taagaagaga
     3781 tagggcgcac taagatgttt tttgtttttc atttttcttt tttagaagga ggagttggga
     3841 actgacttcc caactcctgt atggagcttt attcctgcaa gagaatatcg atttcagccg
     3901 ctgcttcgtc cagagcttct tgaacggatt tttcacctcg taatattaat tgacctgcgt
     3961 cggttatgat ttgatccatt tgctcccatt tgggatgcga tgttcgggat ttcgatgtgg
     4021 tcagttgctc aagaaagacg gtgtagtaag gatgattttg catgtaatct gagttgacgg
     4081 catcttttaa gacgggaatt tgaccaactt ctgccatttt cagttgatat tcttctgaca
     4141 gcagaaagcg catgaactct agagcagctt ctttgttctt cgatttttcg aaaaggacca
     4201 catcttctcc accaacaaca ttgatggatc cgcccggtcc ggcaggaaat gcgctgacgg
     4261 atacttcttt gtcggggaat tgtgattcga tgatcgagaa tgtccatggt ccattatcga
     4321 ggcttgcata gttgtcttga gcataaccgg taaaaacgtc gattccactt ccgagtatca
     4381 cgggggcgat gcatccgtta ttataaagtt ggagcagtga ttcgtaggct gctaccgtac
     4441 ccggaccgtt caaataaccg gtggcggtgg tgattgcgtc gtctgtaatg gatccaccaa
     4501 agctccatat ccatgggagg gtgacccagg caaatgtccc atcagttgcg aaatagtaac
     4561 tatctggata cactgccttg atcgcttcac attgctccaa catttcatcg aatgaggtgg
     4621 ggggcaggat atcggctgct tcatacatcg ctttattata gaaccatgct ttggtattgg
     4681 tatctagcgg taatccatag tagtgcccat tccaggcgtt ggtggaaagc gggccggaga
     4741 aaacctgatc ggcgattgtc tgaaagtctt ctatattttc atccaatgca accaatgcgc
     4801 ccatctctgc aaattccgga acccaaatga tatcgatgcg cgcaagatca ggggcaacgc
     4861 caccggcaat agaagtatat aacttgctcc ggaaatcttc ccacggtacg atcagagtct
     4921 cgaccttgat gtttgggtgc tcttcttcaa aaattggaat gagcgtattt accaatgtgt
     4981 cattttctgg ggaattctcg ctgtaggtgt gccagaatga tagggtgatc acctcttcag
     5041 ccggactttt ttcagcaatc gtttctacct gcgcagtttc ttcccccgtc ttggcttggg
     5101 tgcatccgct gatgacaagg ctgaaaaaaa ttgaacaaat tgtgagaatc atgaatggtt
     5161 ttttcatcat tattaactcc tattcttcgt ttatgtatta tggtagattc tttagtgaat
     5221 atccatcaac cacctccatt tccatacgtg gattgaaacg tttcaattcc ataataaaaa
     5281 aattatgccg aggtcgactc tcgcacgaac agttctggtt ctatcaactt gtgcacaggg
     5341 gcgctctttc cttctaatac gtcaagcagg atattggtga ccatatcccc gattttcttg
     5401 atgggctgtg caatggttgt caaggaaggc tgccagtaat acgcgagcgg aatattatca
     5461 aaaccaacga ttgcgatgtc cctccctggt tttaaagaat tcatctgcat cgctttcatt
     5521 aaaccgaaag cgcatatatc gtttggcgcg atcagtgcgg tgggcacctg cgaggaattc
     5581 aataaagtgt tcatcacttg aatgcctgac tcctcagaaa aacccccttg aatgatcatg
     5641 gaatcatcaa tttctaagtt aagatcgcgc atgcatagat gaaaactttc aagccgttcc
     5701 tttgagcaga ataatccaga ggggggggtg atcaatccta ttcttttatg cccaatggaa
     5761 gccagatatt ccacagcttt cgaaatggct tttgcaccgt ccacatccac ccacgcatgt
     5821 tcattttgcc ttgaggtcct gccaaacgtg acaaaaggaa agttcagatc cagcaagaga
     5881 ttaacgcgct gatcatgtac ttgcgtgcct aacaatacga aaccatcgat ctgtttttct
     5941 ccgatgagtt gtacatattt ttcggtttcg tcgttttcaa ccagaacggt ttcaagcaat
     6001 acgccgtatc ctctctccgc tgcggcagtg ttcactgccg aaagaaaatc aaaaaagaca
     6061 taatcgctgg acacttgttg cttgttttct tcgaccgata tcactggaat gagaccgatt
     6121 ttatgagttt gccgcgattt caatgcccgg gcagcgtagt ggggatggta attcaattgc
     6181 tcgaccactt gtttgatgct tgcgtaagtt tggtcattca gcagatcggg attattgaaa
     6241 tagttagaga tcgtcccgat ggaaaaccct gctttttgcg ctatgtcttt gatcgtagcc
     6301 atgatagaat tgaaacgttt caattattat acagaattct gctgattttg caagaggtgg
     6361 ttgtgcattt taacctggcg cgttcataaa acagcgtcga ggggaatttc gtgtacaatc
     6421 atcctaacca agcgtttaag gagaccacgc acatgaaaaa taaaatcttc ctgactgtta
     6481 tcatccttgc cctggggatc accgcctgcg ttaacaagcc tcaggctagc acaggaagtt
     6541 ccaacctgcc ggaaacggcg ctggcgatcg aaggaccggt gttgttgacc ggctcgttcg
     6601 attacaccaa tgaattcgtt ctggagacct actacgttga acacgcggtg atgctgaccg
     6661 acctgaccag cttcatcctg cgcgatctgg aatgggaatt gccggtggaa tcgcaggtgc
     6721 tgggttatct ggatgtggac gaggatgcca acagagccac ctaccgcctg gcactgccgg
     6781 tgctgcctga aggagaaatg caggatgtgg atcaggatgg caggcaggat agcggagtgc
     6841 aggttttcgc cgtggcatat tcgcccaacc tcaccggtga taccttctcg gtgggggatg
     6901 accgcacctg gggctggcct tcgtacctga cttctgtgaa gaccgatacg gagaacgacg
     6961 atgagatcat cggcggcaag ctggtgattt gggctccgga tgatagccag caattcccca
     7021 gcgggttcgg tgcggatggt ctgctcttta ccgccgatga cccggtgatg gacgtgccgg
     7081 ctggctattc ggtgattgac ctggatacgg atccgttcga gatttaccgt gaccggatcg
     7141 tcgatgtgga attgtacgag ccgcaagatg tcgccatcaa ggattacagc gatctttcct
     7201 acagcgaggc attccagacc atgttcgaca ccatcagcgt ggagtatgcg ttcaatggcg
     7261 tcgagggaaa ggaacctgac tggcaggcgc tgtatgacac ggtgatgccg atggtagaac
     7321 aggcacaaag ggatcgcgac ccccatgcct tcttccaggc gttgcacatg ttcacgctcg
     7381 gctttgatga tggacatgtc tattttgacg gcaacgatta ttattggaat tacctgtacg
     7441 aacaagtttt tcccggcttt ggattttcag ccaaagaaac cgatgctgga gaggtggtgg
     7501 tagtgtatgt taccgccgat agtgaggcgg aacgggcagg catgcaggtt ggcgcgcagg
     7561 tgttgagcgt gaacggcgca gaggtagaag attggatcgc gcagagcata ccgttcttcc
     7621 ccacttcttc cgcccatttg tatcgctatg accaggtcac cagcgctttt cgtgcacctg
     7681 agggggagca gatgaccatc acctaccgca atccggatgg caggaacagg caggtgacgc
     7741 tcacctcgtt ctatgaccca tacagtttca ttaacgtcga ttctggctgg gcgtggtatt
     7801 ttgcatcggt gatgccggtg gagtttgacc tgctctcgga tgatatcgga tatatcaaga
     7861 tcagttccaa cagcgacgac atcgggttga tcatccgcct tttccagcac gcactgcaaa
     7921 ccttcactga ttatggtgcg accaatttga tcattgacat gcgcgtcaac ggaggcggca
     7981 ccaacctggg gctggcggga ttcctgtacg atcagaatat ccccatcggg caactggaat
     8041 actacagcga aaagactggt cgcttcgaag cggaagggct gatggatgaa gtgtatccga
     8101 acgaaaaaca gttccgtttc gagcagatca tcctgctggt ggatcaatac tgcgccagcg
     8161 cctgtgagct ggaagcgtac ggcttcagcc aggtgccagg cgtggtggtg atgggagaga
     8221 atcctaccgc cggcatggaa gcggaggtgg cgcgcggcga attcgttctg ccggagaata
     8281 tcaccctcac cattcccacc gggcgcttca tcaaacaaga tggatcgatc ttgctggaag
     8341 gggtaggggt cgtgccgcag gtgaccatcc ccaccaccat cgaaagcctc accgctgata
     8401 gcgacatgac cttgcaagcc gccattcagt atatctatgg caactattga tcctttcgag
     8461 gtcatgggac agcaaggggc agggagttcc tgccccttgc tatttctggg gaccgttgga
     8521 gtgctggcgc gaacgccgcg ccagcaacac aatggcgctc cagatcaacc cgtagccgct
     8581 gaagaagatg ccccattgaa agccaggggg gacgaaggca gcggaagctt gttgaaaagc
     8641 gatattgctg ccaagcgtgg cgagatcttt gaggtacccg ccttgcagca taaaactggt
     8701 gtaattccag ataaaactga tgaagaccag cagtgcacca cccaacagga gagcccattc
     8761 tccggcattg atcttcactg atgctcctct ctggtggtga aaaagaatga cgcctgccag
     8821 aacgagcatt gccaccgtgc agagcaatgg tgatagaaca ggtccatccc aggtgatggg
     8881 gatgaggaaa aggatatccc attccataag gctggcaggc cagttcagca ggaccttcag
     8941 ccagccatag tagaagatat cccacattgc gaagttgaaa aggaaatatg ccagccaggt
     9001 ggtgaaattt tcggctgcca gtgcgctcac gcataccagc atgagcaggg taccaacttc
     9061 gcgcaacact tcgatcttgt agatgttcca atccatcggt gccagcggaa agcggaagcc
     9121 ctgcgggtaa tacagttcac gcagataaat caccaccact gcttccagca gcgccatgcc
     9181 gaccccatac cagaaagacc aggacacttt tttcagtact ggatgtttcg ttttcatttt
     9241 ctccctttct tgtgcgcaat tataaacgca tccctgcgac attccttgac aggaagacta
     9301 aaacagatat actacaaact agtttgcaat acaaaccata aggtgagaaa gaatgaaaga
     9361 caagatcggg cagatcatgc gcaaaacgta cagctactgg tgggtggatg gcatggcaga
     9421 gatggggata ggcttgttct ttggcatcct ggctgcttac aattacctca tcgagacctt
     9481 gccgctcagt gcaaccgcca gcctgttcat ggcgattgcc cagccgctgg tcttcctggc
     9541 aggttggttt gggtatggtc ggctggtgcg atgggtaaag gaacggttta cataccgtcg
     9601 cacggggtac gtggcttacc agccgaagaa ccgcaaaaat cgcgcagtgc gtgcgctcgt
     9661 cggttttgtg atgggtttcg gcatggcatt gaccgtgaca ttcattggtc ctgaagtgct
     9721 cgagatcgat tcgctgatca tggtcggcgg tttgcttgca ctggtcaccc tctttctggc
     9781 gatctggcat ggggtcaatc gcttgttcgt ggtggctgtg gcggagtttg tgttggggtt
     9841 gtggagttca cgcctggcgc tggaagccga cctgcgctcc atcctgctca tggcatgcat
     9901 tgggggagtt tggttccttt ccggtctgat tacttttgcg ctctatctgg cgcgtacgaa
     9961 gccaggtcag gaggaagcat gaatgatggt gccctgcgcg acctggctga agtagatcgg
    10021 ctcatccacg agcccgcccg gctggtgatc tgcaccatcc tgtacacggt gaaagaggcg
    10081 gatttcctct tcttgctcaa tgaaagcggg ctgaccaagg gcaacctttc ttcgcacctc
    10141 acgaagttgg aggaagcggg ttatattgac atccgtaaga tcttcaacga aaagaagcct
    10201 cagacgctgt gcagcatgac ccccgcaggg cgtagcgcat ttgaagaata ccgcaaatat
    10261 ttcaaaaaga ttatagaaag aatgaatgga gaataaatga caaacgagaa tatcagcatc
    10321 agcacccacg acctgcgctg tcagttcggc gaagtgcgag ccgtggatgg catcaccttc
    10381 gaggtgcaga ccggcacgct cttcggcttc ttgggtccga acggtgccgg caaaaccacc
    10441 actatccgct tgttgttggg cttgcttgag ccagacggcg gaagcgccca ggtgctgggg
    10501 tacgacgtac gcacccaggc agatcagatc agactgaatt gcggtgcgct gctcgaacag
    10561 agcgggctgt acgaacgcct ttcggctgaa gacaacctgg atctcttcgg acgcatctgg
    10621 cacatcccga cggagcagcg cgcccagcgc ataaaagaat tgctggaaaa gatgggatta
    10681 tacgagcggc gcaaggaaac cgtgaacacc tggagccgcg gcatgaagca aaagctggcg
    10741 gtggtacgca cgctcatgca caatccccgc ttggtcttct tggatgaacc gaccgccgga
    10801 ctcgaccctg tggcggctgc cagcctgcgt gatgacctgg tggatctggc gcgccagcat
    10861 agcgtgacgg tctttctgac cacccataac ctggcggagg cggagaaaat ctgcgaccgg
    10921 gtggcagtga tcaacaaagg caaactgctc acggtgggtt cgcccgatga gctgcgcctg
    10981 cagaagagca cccattccct gaccgtggtc gggcgcgggt tcagcccaga ggtggtggct
    11041 gctttgcgtg ccatgcccga ggtagaagcg gtggagctgc aagaccgtca cctggtggtg
    11101 catttcaagc aagagaccga ctcaggcaac ctggtgcagt ttctggcggg caagggcgtg
    11161 atgatcgacg aggtgcgcaa aggcaaagca tccttggaag aagcgtttct ggcattattg
    11221 gaggaaaagg aatgatacag gatatcttga ttttgatccg caaggaatgg aaagaagtga
    11281 tctttcagcg cgcagggggg aggcagagct ggatcagcat cctcattttg ctggcattgc
    11341 tgggagtcta tttaccttac atgaacgggg ttgactggct gtacaacccc accgcgctgt
    11401 ttgcctgggc ttgggtgccg ctgtttatga ccattgggct ggtggtggac gcttttgctg
    11461 gggaacgcga gcgccacacc ctggaaacgc tgctggcgac ccgcctctcg gacaccagta
    11521 ttttgctggg caagattgcc gcctcggtgc tgtatgcctg gagcatttca gtcatttgcg
    11581 cactgctcgg cgccatcacc atcaatgtgc tcttccccag tggaacgttc cagttttata
    11641 acgcgggtat gttcttcggg ggaattgcgg cggtgctgtt gatcgccatt ctcatcagca
    11701 gcattggcgt gttcgtttcg ctcaaagctc ccaccgtacg ccaggcacat cagaagttga
    11761 gcttttccat catggtggtg tggtttgtcc ccttcatcct gctgcaagca tttcctgaca
    11821 aggtgcagga tttctttgtg tcgatcgcac cgctgttgga gaacaacctg ccgctcatca
    11881 tcggcgggtt gttcctgctg ctggtggtgg cagacgctgc cgtgctgctg ctggcgaaaa
    11941 agcgcttcca gcgcacccgg ctgatcttgg aatgagcaag cgaagcggaa gggaagaaat
    12001 ccttccgctt tgaaatgtca ctttacagtg cgtgcttttc aaaatacaat agaaatttgt
    12061 aaatagactt tttggatgct ttttaataat gaaaatcgcc tatgtaaccc tccattggcc
    12121 ccgcttcaat accggtggag taagcaataa aatactccat cagatccatc tttgggaatc
    12181 tcaaggaaat tccatcattg cctacatgca cctgcacacc gttgagaatc cgcaggacct
    12241 gcttcctgcc aggtatttct tttattctag aaaaaaattt cctttgctaa ttccctatca
    12301 agagatcagt cgttccattg ctttattgaa attgatcaat gccttaaaga aggatcaacc
    12361 cgatgtgatc tatttgcgct ggggaatgta cacatttccg ttacaattct tgccgaagaa
    12421 tatgcctatc attatcgaaa tcaataccaa tgacctccat caacatcaat tattgggtcg
    12481 catcttgagt acgtacaaca agatcacacg atcaattaca ttatcgctgg cagatggtat
    12541 ggtatttatt acaaacgaac ttgcagacga tcccgctttc gcgcgtttta ctcctcaacg
    12601 cgtcgtgatt tccaacggaa ttgatcttga tgagtatccg tttttcaaag cccctaaaac
    12661 agatattcct cacctggcgt tcatcggcac ccccggtctg ccatggcaag gtgtggaaaa
    12721 gttggtcgat cttgcgagat tacttcctga tatccatgtc gatatcattg gatacgacac
    12781 gatcccagag gttgacaaga tgccatctaa tcttacctta catggttatc ttaaccaaga
    12841 cgcttaccgt caggtccttg tcaaagccac cgctgccatt ggcactcttt cgttgtataa
    12901 aaaagaaatg caagaagctg ctccacttaa aattcgagaa tgcgccgcct atgggatacc
    12961 attgatcctt ccatacaaag acaccgacct gcatggacaa aagtgtgatg caattttaga
    13021 attgccaaat accgaagaca atatcactga gaatgcacag cgcattcacg attttctcta
    13081 tcgcatgcaa gggaggcgat tggaacgaaa gatcatctat cccttgcttg atattaaaca
    13141 gaaggaatta gccagattga acttcttcag atcattcata aaatagtaaa taaaaatctt
    13201 tctcgtttga acttactagt taaactgtta caaatttatg agattgttct tccattgctt
    13261 aaattttttg ttgagataag tgttaccctt taaaaccgat ccataaatga ttgagccacc
    13321 actgtgtcgg aaaataatgc cgctccagat tcgaaaaccc tgaattttcc aggcatccct
    13381 tgatctcttc atccaaaaca aaaaagctct cgccttcagc cattccgcgc tctgaatcca
    13441 tatcaatgtt cgttccgaaa aacttgtctt gcaaccacac attcaaatga gcaagcaatt
    13501 ctgctatcgg actccccata gtaacaatga ttctaccgcc ccgttctaca atgcggtacg
    13561 cttctctgat ctcctggctg cgcagatctc tgggaatgtg gttgaaattc gcaataaaag
    13621 taaccgtgcc aaaactatta tcggggtatg gcagttcgca gaaagtttca acaagattgt
    13681 cctcggttaa tccctcataa ggaaaaatat ctaaaccgat cccgtgattg ttgaggaatt
    13741 gtttgatgaa gatattgttc tttccacaac caatgtccaa gcaccttcca accacatatt
    13801 tttttacata ccggaaccgc tcatcgcgca gtgatgtcag tccgaactta ctctcttcaa
    13861 ctaaaaatat ggctctcagt ggaaacaata aaaaatcttt gattggggaa tattctttcg
    13921 gatttcgttt aatcattata tatgcctact cattatcaaa gtcttgttat gcttaccatt
    13981 tttcgtaatc gatatatcgc acaaaataat ctcctaaaag tgaaaattga tttaaagaca
    14041 ttcgctgtta cgacaggctg gtaaactcaa ttaattatat tctccaaact attccggtaa
    14101 agatttcgcc tatcggcaaa gcaagatttg ttaaagtata attgctcacg acctttttca
    14161 cacaagagtg acacaaatca aatgcttaaa gaatatttat ctcaaaaatc cacagagaga
    14221 ggcatattaa ccgtagctgg agtattgatc gccgcttgtt tcacgcatca cgtgttctcg
    14281 atttgggaca gaaaagcgct ctggattctt cttgcttttt tactcctttc tcctttgttc
    14341 acgctcgttc tatcctttat ctatgcccaa tctaaatcag ctttccagca aatacaacgc
    14401 aagcgctctc tcttcctcat ttctgcactg gttcttggat cgctcacaac ctgggcaacc
    14461 tatcgggctc ccgtttcata ccagacgatt acgctcgaac cccaactgta cgctcatcaa
    14521 agaatcgagt tgctggaaat caaggtggga ggagatatct attcctttga agaaaaagcc
    14581 cttgaagccg gctggaatct cgaagatgga atgttcaccg ctacacaaga ttctgatcca
    14641 ttaatattaa cctttccggt tcccttgcga tcaaaggtaa acatcctctt caattccagc
    14701 gtgcaaagcg gaagggtttc catatcctat ggcagcatga gaagagcggt tgatttatca
    14761 acctccaacc ctcaacagaa actcataacc ttgcacagcc aatatcgcac tgttccgaat
    14821 tggttgttcg ttcccttctt ggttattgca gacgtgttta cctttagctt tctatttttg
    14881 ttgatcttta ttctccagga aaaagggcag cgtaaactta tcgaggatca tcgtccagaa
    14941 aaatttttca gccaccagca atgtgttctc attctggtct cagttgcggt catccttcac
    15001 ctgctcaatg cgctggcagt gcccttgatc gttgacagcg attctccggc atatcttcaa
    15061 ggagccgtcc atttattgca atatggaaat ctaaagggtg tttctgtgtt ctgtggaccc
    15121 ggaaccactt tccttttcgc tccggtgttg tatgtgtttg gaagaaatcc atgggggatg
    15181 aaaattctat tgcacctgat tgcaatagcc agcgtcttgg tcagctaccg catcggctgg
    15241 cagttaagcc acaagcgctg ggtagctttt tgcatcggct ttttgacaat actgataccc
    15301 gaattctatt attattccaa ttttgttatg agcgaaattc ccaatatctt tctcatcctg
    15361 ctatcttcca gcctgtttct ggatgccctg gttgatttca gctttcagcg aattttgctt
    15421 gcctttgggg tggtaagctt agccacgctc ttgcgtgcgg agaacatttt ggtcttcctc
    15481 atcggcgcag tctgcctggg atccaaaccc gcctgggatt ggttcaacgg ttttttcgat
    15541 aaaacaaaga aacaacaaaa acatagcagt cacccaccgt tggctatgct gttttgggct
    15601 gccttgatcg cgctcatccc ggtgatatgg ttgtcgctct tcaattatca ggcgcatggt
    15661 tttctggggt tgaacagttc ttcgggggta gtgctttatg gcggttgggt gtattatccc
    15721 gaagccagcg gctttgattt tcgtgatgaa agctcccctg cagttcaaga aattgaccgc
    15781 tggatagaga aatatcccat cgacatcacc gatggcagcg gtatcgccac gccgggagaa
    15841 atttacccca gcttgataaa agcgggcttt t
//
LOCUS       DAOSDS010000047        15764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1499_length_15764_cov_4.520084, whole genome shotgun sequence.
ACCESSION   DAOSDS010000047 DAOSDS010000000
VERSION     DAOSDS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15764
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1499_length_15764_cov_4.520084"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLL88_06810"
     CDS             complement(<1..757)
                     /locus_tag="PLL88_06810"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOJ01316.1"
                     /translation="MRAVDVIIRKRDKQELTKDEIEFFIQGVTSASIPDYQISAWAMA
                     VLLNGMTPQETTYLTLVMAHSGDILDLSSVAPLVVDKHSSGGVGDKTTLVVAPIVASF
                     GLPVGKMSGRGLGFSGGTLDKMESIPGYRVNLSEAEFMRQLKEIGIVLTGQTSDLAPA
                     DGILYGLRDVTGTVPSTPLIASSIMSKKIAAGAQAIVLDVKLGYGAFMKDLKHAQELA
                     RLMVEIGNLSGRKVRALLSDMNQPLGEAVGNAIE"
     gene            complement(797..1336)
                     /locus_tag="PLL88_06815"
     CDS             complement(797..1336)
                     /locus_tag="PLL88_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HOJ01317.1"
                     /translation="MTEANLSNQIPSYPDDIADKFEKAQQVAHETLTALLEKMSVDAN
                     VVTRIIDPEDDKDQSIILADISGKDLSILIGRKSETLNSLQYITSLILCNEIGEWVPL
                     MVDVQGYRYRRERQLRQLARRIADQVVQTGRRQVLEPMPANERRIIHLELRYHPFVTT
                     ESTGEEPNRRTTIFLKPRE"
     gene            complement(1537..2349)
                     /locus_tag="PLL88_06820"
     CDS             complement(1537..2349)
                     /locus_tag="PLL88_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOJ01318.1"
                     /translation="MWDSLIIKPFINSLLLIYNLTGESFGIAIIVFTVLIKAITHPLI
                     ARQIKSSAALQDLQKDEEYLEIQKKYKNDKDRLGQAQMELYKKKGIKPFASCLPTLLQ
                     LPIIIGLYQSIIQTMGTAPLQVFNLVSKIEMGLSNLFSFIPAAKNPYTLIPLNNHFLW
                     MDLSQPDRVYIPGISFGIPVLAIFVVITSFIQNKLISPSGGDQQANQATGMMNIYMPL
                     LMGWFAYSLSSGLALYFAISNVISLVQYAVLGRVKLKDIFKKKTAPEKKPAK"
     gene            complement(2368..3489)
                     /locus_tag="PLL88_06825"
     CDS             complement(2368..3489)
                     /locus_tag="PLL88_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOJ01319.1"
                     /translation="MKKNITIAALLILIISAMLAGCTGNVGTSSSWPGFSVDNDLAVV
                     SYGYQLYALNVQNGSKTWVFPAEQNSKIIFYAPVEFAENLVIAGDYTKNLYALNSSNG
                     ILQWQFDGAESRYVASAFYKNGYIYAPNADQKLYVLDQNGVLQWIFETAGANWSKPVA
                     DEQYLYLASMDHNIYALNLTYASSDLVAGKDGTRNRVEEPVWKTDLGTAVVSDPLLAD
                     GFLYAGTIDGKMFKVSASTGEIVWKFEGEAKIKSIWTKPVILDNVVFFATEDGSIYAL
                     NAETGAPVWDAPMPTGSQIVAGGVVINDTVGFGTLGGNLVIVDKDQVTSPSLSREGAV
                     YSTPVYKDGKLYLVMVNGEKLVYALDENGREYWTFSTEE"
     gene            complement(3515..3796)
                     /gene="yidD"
                     /locus_tag="PLL88_06830"
     CDS             complement(3515..3796)
                     /gene="yidD"
                     /locus_tag="PLL88_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOJ01320.1"
                     /translation="MIAKENQYQEPSLREVKVTVWTFPRYIILFLIRVYQKTLSPALP
                     ANTCRFYPTCSHYAYQAIFRYGIWKGGYLSIHRLIRCNPFNEGGYDPVP"
     gene            complement(3786..4145)
                     /gene="rnpA"
                     /locus_tag="PLL88_06835"
     CDS             complement(3786..4145)
                     /gene="rnpA"
                     /locus_tag="PLL88_06835"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOJ01321.1"
                     /translation="MNRKFRLTRSADFKKIKDSGNVYFHPIVKMAVRRNELPHSRFAV
                     ITSKIIGSAVERNRCKRRVRAVLNLLRNNCEAGWDIVFIIRKDFNHSSASEVQTAVEN
                     LFSQAGLLTTKEKIYDR"
     gene            complement(4188..4358)
                     /gene="rpmH"
                     /locus_tag="PLL88_06840"
     CDS             complement(4188..4358)
                     /gene="rpmH"
                     /locus_tag="PLL88_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOJ01322.1"
                     /translation="MPKRTYQPRIRKRVRVHGFRQRMATAAGRKVLKRRMARGRHNLA
                     VTKNNHVKNVRW"
     gene            complement(4411..5727)
                     /locus_tag="PLL88_06845"
     CDS             complement(4411..5727)
                     /locus_tag="PLL88_06845"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOJ01323.1"
                     /translation="MKVFLGSIGCRLNQSELEILARELRAAGHEIVAAPEHARAAIIN
                     SCTVTAAAEADSRQLVHRLQRLGVGEIYLTGCWVSLDVVGEEPLPEGVFVVSNRDKMN
                     IVQKYFDATVDRKPDASGRVPLPGKAKRTRAFIKVQEGCDYHCTFCITRVARGKSVSR
                     PLHEIMKDIEAATIADVKEVVLTGTQLGGWGRDLPEKQTIAFLVNTILETTNIPRVRL
                     SSIEPWDIHPDFFPLLTNPRFCAHLHLPLQSGATNTLHRMRRQMLPVAFQTLVNKIRS
                     FNPHIALTTDIVVGFPGENEEEFMESLQFVQKMNFAGGHVFRYSQRAGTPAAEYPDQV
                     PGLEKRARNEQMRAVLQRSSDQYRDRFLHCTVPVLWERAKKIGDAEWMMEGLSANYLR
                     VQAQSNKDMWNMISLVELTANAGKSMLGEIVSVEGEQSYRENINMV"
     gene            complement(5730..6374)
                     /locus_tag="PLL88_06850"
     CDS             complement(5730..6374)
                     /locus_tag="PLL88_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01324.1"
                     /translation="MQSRFLVFFLLTLCVTMAGCASFFPSVYQSGDLLIVDDFSSPNL
                     EWNVWKRADGSTVGYYQEGLVFTINSQNTDYVSVLNSTYSDVRIETVARNLNGLLDNG
                     FGVVCRYLDDSNYYAFLVSSDGYYGILRVLYGGYAVLNGGELQYSSVIAPGEATNHIR
                     ADCIGNQLTLYVNDVMLARVQDDVFSEGQIGLTASSFRETGVAVLFENFVAVYP"
     gene            complement(6452..7081)
                     /locus_tag="PLL88_06855"
     CDS             complement(6452..7081)
                     /locus_tag="PLL88_06855"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOJ01325.1"
                     /translation="MNTKVDQETTNKKKKSGLFKFVILLVVLIFGSLLLRTMGSYLIV
                     SNELEYANAIVVLSGGDESRMQEALELYNQNYSNLIILTETGNVVEGYDHLHSFDMRI
                     ELLNNGIPSGNILLTDQVATSTQEEAEAVKRLLITRQSISCIVVTDPYHTRRAYLIFR
                     DVFADTGIKVMIHPTSPHWFSANTWFLKINGWRFVVLEYLKLFAYQLGK"
     gene            complement(7113..8270)
                     /locus_tag="PLL88_06860"
     CDS             complement(7113..8270)
                     /locus_tag="PLL88_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOJ01326.1"
                     /translation="MAQNFDASIDRLHSDSVKWNTFSKDVLPMWVADMDFRSPPAVIE
                     TLTERVKHGVFGYAAAPQDLDEIIVRWIADQYQMQVEPEQILYVRGVITGLNLASRAF
                     LSPGDGLMFQPPIYPPFFGVGASSGLENHEVNLTQKSDGTYGIDFDELEKGLKKNTKM
                     FLLCNPHNPVGRVFTKEELTRLADFCLRHDLLLCSDEIHCDLVYHGNTHIPIMSLNGE
                     IAQRTVMLIAPSKTFNIAGLGFSVMVIKNKNMMQQVKKNFQGILPHPDLLAAQAARAA
                     YLSGLPWLRELITYLQGNRDYLHDFVRKELGDISMVSPEGTFLAWLDCRKMPFRDDPF
                     SFFLTHAKVGLNNGVDFGNAGKGFVRLNFACPRATLAEGLSRMRNAIHNQT"
     gene            8590..9480
                     /locus_tag="PLL88_06865"
     CDS             8590..9480
                     /locus_tag="PLL88_06865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ01327.1"
                     /translation="MDKIIKDIPLTITRGWENYALLDSGNGMKLERYGRILLSRPEPE
                     AFWTKSLPEQEWKKADAVYFSPSQDENGTWKFNKQFPNPWDIRVGNVRCKLELTSSRH
                     IGLFPEQFPQWQWLHAHLTNSPHPLKMLNLFGYTGMATLFAAQAGAQVTHVDASKKAV
                     SWAKTNQEISNLQEKPIRWIVDDALKFIKREARRQNFYDAIVLDPPKFGRGPNGEVWE
                     FYKYLPNLLQGCEEILSPQPSFLLLTAYAVKASALTLLRALQEQMQAHGGSITAGELV
                     QQEASSQRYLSRAIYALWEK"
     gene            9498..10418
                     /locus_tag="PLL88_06870"
     CDS             9498..10418
                     /locus_tag="PLL88_06870"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HOJ01328.1"
                     /translation="MDPILHTYSIIARDERSGQFGAAVQSHWFNVGSLCPWVEAGVGA
                     VATQSSVKVSYGPQGLKLMREGKSAPEALQKLLARDEDRENRQVAMVDALGNVAVHTG
                     TRCIAQAGHVAGGGFSVQANMMKTDTVWDAMAEAFQKTKGDLAERMLASLQAAQAEGG
                     DIRGKQSAAMLVADGVRSEEPYEHILLDLRVDDHPEPLVELERLLHIQRAYEHMNKGD
                     DFLTKGEMVEAMKEYSLGANMAPAIEELPFWQAVTMAANGYLSEALPIFHSVFKTNKD
                     WAETLRRLPAAGFFPDDKEMLDTILQELKR"
     gene            10430..11257
                     /locus_tag="PLL88_06875"
     CDS             10430..11257
                     /locus_tag="PLL88_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HOJ01329.1"
                     /translation="MQQYRVDPEKPIKIKKLDADDLGEWEGKKKAGQKAFADLQKELA
                     KLQEVLYAEHKHKVLIILQAMDTGGKDGTIRSLLKEVNPQGVKIASFKVPTPVELAHD
                     YLWRVHSMVPAQGEMVLFNRSHYEDVLVVRVHELVSKEVWKKRFEQINAFEKMLTEEG
                     TTILKFYLHIDKEEQKQRFIERLTNPKKQWKFNPNDVEERKNWDVYQEAYQDVLNKTS
                     TSWAPWYAIPANRNWYRDLCVATIVTDALRSLKAKYPEPIPDPETYIQALENEDMEK"
     gene            11330..11530
                     /locus_tag="PLL88_06880"
     CDS             11330..11530
                     /locus_tag="PLL88_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01330.1"
                     /translation="MIEPISTTGIPSFAKGVIIGFADVTSKVTELISSIGMNQLKMKN
                     GSGRAGQIHKPRFGANQPARAH"
     gene            complement(11556..11921)
                     /locus_tag="PLL88_06885"
     CDS             complement(11556..11921)
                     /locus_tag="PLL88_06885"
                     /inference="COORDINATES: protein motif:HMM:NF027272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4751 family protein"
                     /protein_id="HOJ01331.1"
                     /translation="MSKYTKPVFVNQLLSDRSVKTVSKFIAYKTWNDPSLWFAEVIDN
                     GNAFFHWHQGKRNEGHKDSIINYITADGTQWQATIKDYVFFHSPGGDTSKGHSDTVIA
                     YMSGDNRAYEGSFAEWFDE"
     gene            complement(12300..14504)
                     /gene="selD"
                     /locus_tag="PLL88_06890"
     CDS             complement(12300..14504)
                     /gene="selD"
                     /locus_tag="PLL88_06890"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOJ01332.1"
                     /translation="MQKPVYLDYNATTPIAPQVAEAMRPFIYENFGNPSSSHAYGSAA
                     KKGVEKARRQVAEMLGCQPDEIVFTSGGSEANNLALKGVAYAYRDKGNHIITCAVEHP
                     AVLDVVAYLQKQGFQVTIFPVDEFGLVNPHDVESSITPDTILISIMHANNEVGTVEPV
                     REIAMIAHAHGVLMHTDAAQSVGKIAVNVGELGVDLLSIAGHKLYAPKGIGALFIRRG
                     VRVEKIMHGAEHEMNKRAGTENVIEIAGLGQACELIKENLPQYQAHMLAMRTHLENGL
                     RAAFSNVRFNGHPEKRLPNTTNVSFYGLEANTILAELDQVAASAGAACHADQVDVSPV
                     IAAMGIPVEYAMGTIRLSVGRNTTQEEIDFALEELKRVIGNLQAQEQVVFKSGSTEAI
                     KLTHYTHGLGCACKLRPQLLEEILAEIKPSKDKRILVGTETGDDAAVYRINSETAIVQ
                     TVDFFTPIVDDPYLFGAISAANSLSDIYAMGATPLFALNIVGFPSNRLPVSVLREILR
                     GAQEKANEAGIAIIGGHTVDDTEPKYGMAVTGVVHPEKLITNAKAQAGDHLLLTKPIG
                     TGILTTALKQGLLEKEQTDQLIQTMALLNQKAGAALQQFHAHACTDVTGFGLLGHLLG
                     MMKAANLTAVLDFGSIPILEGTYDLAAAGIIPGGSHNNHAYTKPYVRYAERLTEIQQI
                     ILNDAQTSGGLLVAVEHSNAFGMTQYLNDEGVPAVDIGQIAERQGDRYIVLH"
     gene            complement(14513..15313)
                     /locus_tag="PLL88_06895"
     CDS             complement(14513..15313)
                     /locus_tag="PLL88_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01333.1"
                     /translation="MEKIKLNVKIFFEGAYLSYVGLFRWLSPSTYLATLIFSPLTQML
                     FFVLIGTYAQGAGRAYFYVIGNALMNAAMSGIYGVTMSIGGDRWDGTLPYLFGAPANR
                     MTVFLGRAFMHILNGMIGVITGLFWGWLLFHIDYSATNPWALLLTILVTTFSTAGLGL
                     LMGCIGLITRNVMFVNNTVYFVLLVFSGANLELTSLPKWMQIVSNYLPLTRGIASARL
                     IIGGHALGEVLPLLVEEVGIGLFFALLGYSLFRWFEVTAKKRGTLEVF"
     gene            complement(15319..>15764)
                     /locus_tag="PLL88_06900"
     CDS             complement(15319..>15764)
                     /locus_tag="PLL88_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01334.1"
                     /translation="IVCCYAFISLLMGLPLNIAKPWLFAGSIVLTVVAFIAFGLIIGP
                     LFILNPDVQRFQNGLEFVVYIFSGFLFPIALLPMWTTPFSYILTPYWAARALHAASGG
                     TGSDAEIYFCWAMLVVFSLIYAIISKFLFKSILRKAREDATLNMQ"
BASE COUNT         4350 a         3942 c         3459 g         4013 t
ORIGIN      
        1 gttcgatggc attcccaacc gcttccccca gcggttgatt catatccgaa agtagtgcac
       61 gaacttttcg tccactcaaa ttcccgatct ccaccatcaa acgtgccagt tcctgggcat
      121 gtttcaggtc tttcatgaac gccccataac cgagctttac atccagcaca atggcttgcg
      181 cacctgctgc gatcttctta ctcataatag aagaagcaat cagcggagtg gaagggaccg
      241 ttccggttac atctcttaat ccgtacagta ttccatcagc cggcgcaaga tcactggttt
      301 gtccggtcaa gacaatgcca atttctttta gttggcgcat gaattctgct tcactcagat
      361 tcacccgata gccggggatg gattccattt tatccagcgt tccaccgctg aatcccagcc
      421 ctctgcccga catcttgcca accggtaatc caaaagaagc cacaatgggt gcaacaacca
      481 gagtggtttt gtcgcccacg ccgcctgaag agtgtttatc caccaccaac ggagcgaccg
      541 aggaaaggtc aaggatatcc cctgagtgag ccataaccag tgtcagatag gtggtctcct
      601 ggggggtcat accattcaac aaaacagcca tcgcccaggc ggagatttga taatccggga
      661 tggaagcaga cgttacgccc tggatgaaga attcaatctc atctttggtg agctcctgtt
      721 tatctctctt tcggatgatg acatcaactg cacgcatacc actgcctcct ctattttccc
      781 gaacgcaaca gaatggttat tctcgaggtt tcaagaaaat ggtagtacga cggttgggct
      841 cttcaccggt gctttcggta gttacaaatg gatggtaacg gagttcaagg tggatgattc
      901 tgcgctcatt ggctggcatc ggttcaagca cctgcctgcg tccggtttga accacctgat
      961 cagcgatacg tctggcaagc tgacgtaact ggcgttcgcg gcgataacga tatccctgca
     1021 catccaccat caaaggcacc cattctccga tttcattaca cagaatcaag cttgtaatat
     1081 actggagaga attcaaagtc tctgacttgc gaccgattaa tatgctcaga tccttgcctg
     1141 aaatatcagc caggataatg gattgatcct tgtcatcttc aggatcaata atacgggtga
     1201 ccacatttgc atcaacactc attttttcca gcaatgcggt cagcgtttca tgcgctacct
     1261 gctgggcttt ttcaaattta tccgcgatgt catcagggta tgaggggatt tggtttgaca
     1321 ggtttgcttc agtcatttgt cctttcatgg atacgatctt cctttccctt ttcagcgctt
     1381 caggggtttc ttcttaatca ttctcaactt ccaacgcatt cagaatctag aaatacaaaa
     1441 agttccacta ccccttcttg caaaggggta gaaacctcta aagcattttt gattgaattc
     1501 ccaatagcat tgacaacgtc ataattttca agccgcctat ttggctggct ttttttctgg
     1561 cgctgttttc ttcttgaaga tatcttttaa tttcacgcgt cccagtaccg catattgcac
     1621 caaactgatc acgttggaga tggcgaaata caatgccaga ccggaagaaa gcgaataggc
     1681 aaaccaaccc attaacaacg gcatgtagat attcatcata ccagtcgcct ggttggcttg
     1741 ctgatcaccc ccagagggac tgatcagttt gttttgaatg aaactagtga ttacaacgaa
     1801 aatcgccaac acaggaattc caaatgaaat acccggaata taaacgcggt caggctgact
     1861 gaggtccatc caaagaaaat gattgttcaa tgggatcaat gtgtaaggat tcttcgctgc
     1921 tggaatgaaa gagaacaagt ttgataatcc catttctatt ttggatacca ggttgaagac
     1981 ctgtaatggt gctgttccca tagtttgaat gatgctctga taaagaccaa tgatgatcgg
     2041 aagttgaagc aaggtcggca ggcaagaagc aaaaggtttg atgcccttct tcttgtacaa
     2101 ttccatttgc gcctgtccca gacggtcctt gtcgttcttg tacttttttt gaatttcaag
     2161 atattcttcg tctttttgaa ggtcctgaag agccgcagag cttttgatct ggcgtgcgat
     2221 cagtggatgc gtgattgctt tgatcaaaac ggtaaaaaca atgatcgcga taccaaaact
     2281 ttctccggtc aggttataga tcaacaatag agaattaata aagggtttga tgataagtga
     2341 atcccacatg gttttttcct tttctattta ttcttctgta gaaaatgtcc agtattcgcg
     2401 accattttca tccagtgcat aaaccaattt ttctccgtta accattacaa ggtaaagttt
     2461 tccatccttg taaaccggag ttgaataaac tgcaccttcc cggctcagcg aaggtgaggt
     2521 tacctgatct ttatctacga tcaccagatt tccaccgagc gtaccaaaac caactgtgtc
     2581 gttgataaca acccctccag ccacgatctg tgaaccggtg ggcatgggag catcccaaac
     2641 aggcgccccg gtttcggcat tcaaagcgta aatacttcca tcttcggttg cgaagaacac
     2701 tacattgtca agaataacgg gctttgtcca aatacttttg atcttggctt caccttcaaa
     2761 tttccaaacg atctcaccag tgcttgcact gaccttgaac atttttccat caatggtacc
     2821 ggcatagaga aaaccgtctg ctaataatgg atcggaaaca accgccgttc ccaggtcggt
     2881 tttccagacc ggttcttcaa cacgatttct tgttccgtcc tttccggcta ccaaatcgga
     2941 agatgcatag gtcaagttca atgcgtagat gttgtggtcc atcgaagcca gataaagata
     3001 ttgttcgtct gccacaggtt ttgaccagtt ggcgccagcg gtttcaaaga tccattgcaa
     3061 gactccattt tggtcgagca catacaattt ctgatcagca ttcggggcat aaatatagcc
     3121 attcttatag aaagcactgg caacatagcg gctttctgct ccatcgaact gccactgcaa
     3181 tatcccgttg ctggaattca acgcatataa gttctttgta taatcaccag ctatgaccag
     3241 attttctgcg aattcaacag gagcatagaa aatgatttta gaattctgtt ctgcaggaaa
     3301 aacccatgtt ttacttccat tttgaacatt taaagcatag agttgatatc catacgagac
     3361 aactgccaga tcattatcaa ccgaaaaacc gggccagcta gacgatgtgc caacattgcc
     3421 agtgcacccg gcaagcatag cagagatgat caagattaaa agtgctgcaa tagtaatgtt
     3481 ttttttcatt ttttcaccta gttttttctt tctcctatgg gacaggatca tatccgccct
     3541 cattgaaagg attacaacgg attaaacgat gaattgataa ataaccgcct ttccaaattc
     3601 catagcggaa aatcgcttga tatgcataat gagaacaagt gggatagaaa cggcaagtat
     3661 tagccggcag tgccggggat agcgttttct gataaacacg gatcaagaac aaaatgatat
     3721 agcggggaaa tgtccaaacc gtaaccttaa cctcacgtaa agatggttct tgatattgat
     3781 tctctttagc gatcataaat tttttctttt gttgtcaaca gacctgcttg agaaaacaga
     3841 ttttccacag ctgtctgtac ttcgctcgca cttgaatgat taaaatcttt acggataata
     3901 aagacaatat cccatccagc ttcacaatta tttctcagaa ggttcaatac agctcttacc
     3961 cttctcttac aacgatttct ttctaccgca ctcccgataa ttttactggt tatcaccgca
     4021 aatcgcgaat gtggcagttc atttcgacga actgccattt tcacgatcgg atgaaaataa
     4081 acattcccag aatcctttat ctttttaaag tccgcactcc tagtaagacg gaattttcgg
     4141 ttcactcaat gctcaagcga ttattaaatc aattcttatt ctgggatcta ccagcgaacg
     4201 ttttttacgt gattattctt ggtgacagct aaattatgac gcccacgagc cattcttctc
     4261 ttcagaactt ttctgccagc agcagttgcc atgcgttgcc tgaaaccatg gacacgaacg
     4321 cgttttcgta tacgtggttg gtatgtcctt tttggcatgc tttaattcct cttcttctcg
     4381 caataaattt cttttggtat agcggaagaa ttataccata ttgatatttt cacggtacga
     4441 ttgttctcct tccactgata cgatttcccc aagcatagat tttcctgcat tcgctgtcaa
     4501 ctccaccaat gagatcatgt tccacatgtc tttattggat tgcgcttgca cgcgtaaata
     4561 atttgccgat aacccttcca tcatccactc agcatcacct atcttttttg cacgttccca
     4621 aagcacagga acagtgcaat ggagaaatcg atcgcgatat tgatcggatg atctttgcag
     4681 aaccgcgcgc atctgttcgt tgcgtgctct tttttcaagg ccagggactt gatcggggta
     4741 ttctgccgct ggcgttccag cgcgttggga ataccgaaag acatgccctc ctgcaaaatt
     4801 catcttctgc acaaattgca ggctttccat aaattcctct tcattttcac ccggaaaacc
     4861 caccacaata tcggttgtaa gtgcaatgtg gggattaaac gatcgaatct tattcaccag
     4921 agtttgaaac gcaaccggaa gcatttgcct tctcattcta tgcaacgtgt tggttgctcc
     4981 agattgcaaa gggagatgca agtgagcgca aaaacgtgga tttgtaagca gcggaaaaaa
     5041 atcagggtga atatcccatg gctcaatgga actcagccgc acacggggaa tgttcgttgt
     5101 ctcaagaatg gtgttaacca gaaaggcgat cgtctgtttc tcaggcaggt ccctgcccca
     5161 tccgcccaac tgcgtacccg tcaacaccac ttccttcaca tcagcaatcg ttgctgcttc
     5221 aatatctttc atgatttcat gcagtgggcg gctgacactc ttgccacgag caacccgcgt
     5281 gatgcagaat gtgcagtggt agtcacaacc ttcctgcact tttataaacg ccctcgtacg
     5341 ttttgccttt ccaggcaagg gaacgcgtcc agatgcgtca ggcttcctat ctacggtagc
     5401 gtcaaaatat ttctgaacga tgttcatttt atcccgattg gacaccacga acacaccttc
     5461 aggcagcggt tcttcaccca caacgtccaa tgaaacccaa caaccagtca ggtagatctc
     5521 gcccaccccc aaccgttgaa ggcggtgaac caactgacgg gaatccgctt ctgcggcagc
     5581 ggtcactgtg catgaattga tgatagccgc tctggcatgc tcgggtgcag ctacaatttc
     5641 atgaccagcg gcgcgcagct ctctcgctaa tatctccaat tcgctttgat taaggcgaca
     5701 accgattgac ccaagaaaga ctttcatgct tatggataaa ccgccacaaa gttctcgaac
     5761 aaaaccgcca cccctgtttc tctaaaagag ctggcggtta gaccgatttg cccctcagaa
     5821 aagacatcat cttgcacgcg tgccaacatg acatcgttca catacagggt caactggttt
     5881 ccaatgcaat cagcgcgaat atgattggtg gcttcccctg gtgcaattac gctggaatac
     5941 tgcaattccc cgccatttaa caccgcatac ccaccataca gcacacgcag aattccataa
     6001 taaccatctg aagataccag gaaggcgtaa taatttgaat cgtccagata gcggcagaca
     6061 accccgaacc cattatccag caaaccattc agattccttg ccacggtttc aatacgtaca
     6121 tcggaatagg tgctgttcaa cacgctgaca taatcggtat tttgcgaatt gatggtgaac
     6181 accaatccct cctgataata acctacagta gaaccatcag cacgcttcca cacattccac
     6241 tctaaattgg gggatgaaaa atcgtcaaca atcagcaagt cccccgattg ataaaccgat
     6301 gggaaaaagc ttgcgcaacc tgccatagtt acgcagaggg tcagcaggaa aaacacaaga
     6361 aatctacttt gcatgtggaa ggacttcctc aaatcatcca ttatacccat caaccattct
     6421 ctcatctctg aacgaatctt cacggaaaag ttcactttcc caattgataa gcaaagagtt
     6481 tcaggtattc cagaacaaca aaccgccatc cgttaatttt caagaaccag gtatttgcac
     6541 taaaccaatg cggtgaggtg gggtggatca tgactttaat tccagtatca gcaaaaacat
     6601 cccggaagat caaatatgcc ctgcgggtgt gatatggatc ggtaaccaca atgcaggata
     6661 ttgattggcg ggtaatcagc aagcgtttca cagcttcagc ttcctcttgt gtgctggtgg
     6721 caacttgatc ggtcaacagg atatttccac tggggatgcc gttgttgagc aattctatac
     6781 gcatatcgaa actatgcaaa tgatcgtacc cctcaaccac attgcctgtt tcggtgagga
     6841 tgatcagatt ggaatagttc tgattataca gttccagcgc ttcttgcata cggctttcat
     6901 ctccaccgct caagaccaca atggcattgg catattccag ctcattggag acaatgagat
     6961 aggaacccat ggttctcaaa agcaggctac cgaaaataag aaccacaagc aggataacaa
     7021 atttaaacaa cccgcttttt ttcttcttgt ttgtagtttc ttgatctact tttgtattca
     7081 tagcaatgac aatcctacca gaaatagcaa aactatgtct gattgtgaat agcgtttctc
     7141 atccgcgata gcccttctgc caacgttgca cgagggcagg cgaaattcaa ccgcacaaat
     7201 ccctttccgg catttccaaa atcgactcca ttattcaatc ccaccttcgc atgagtcaga
     7261 aaaaaggaga acgggtcgtc gcgaaatggc atttttcggc aatccaacca agccagaaat
     7321 gtgccctccg gactgaccat ggaaatatca cccaattctt ttctgacaaa atcatgcagg
     7381 taatcgcgat ttccctgtaa ataggtgatc aattcccgta accagggcaa tcctgaaagg
     7441 taagcagcac gggcagcctg tgccgccaga agatctggat ggggtaatat tccttgaaag
     7501 ttttttttca cctgttgcat catattttta ttcttaataa ccatcaccga aaaacccaaa
     7561 ccagcgatgt tgaaagtttt gctgggcgcg atcaacatga cagtacgctg cgcgatctct
     7621 ccatttaagg acataatcgg gatatgggtg ttgccgtgat agaccaaatc acaatggatc
     7681 tcatcagaac ataataacag atcgtgccgc aaacaaaaat ccgcaagccg cgtcaattct
     7741 tctttggtga aaactcgacc aaccggatta tgcggattgc acaacaggaa cattttcgta
     7801 tttttcttca atcccttttc caactcatcg aaatcaatgc cataggtacc atccgatttt
     7861 tgggtcaggt ttacttcatg attctccaaa ccgctagacg ctcccacgcc aaagaaaggt
     7921 gggtagatag ggggttggaa catcaatcca tcaccaggag agaggaaggc acggctcgcc
     7981 aaattcaacc cggtaatcac accccgcaca tacaggattt gttccggctc gacctgcatc
     8041 tgatattgat cagcgatcca gcgtactata atttcatcca ggtcttgcgg tgcagcggca
     8101 tagccgaaaa ctccatgctt gaccctttct gtcaacgttt cgatcacagc gggtggtgaa
     8161 cgaaaatcca tatccgctac ccacataggc agcacatctt tggaaaaggt attccatttt
     8221 acagaatcgc tgtgcagtcg atcaattgaa gcatcaaagt tttgcgccat gttccctcga
     8281 tttgttaaca accctattct actttaatct ctctgattta ttttcccacg gatttgcttc
     8341 tgacaggagc atctgcttcc ggagaaaaaa gcaagcgata aaacaaaccg ttttagattg
     8401 atacccctga ttcatttgcg aaaaatttga tcgttatgaa taactggata tcatccctca
     8461 taacgtcttt tctcccgcaa tgcctttcaa gaggttccct tcatatccta tgcaatcgtc
     8521 caatcccttt gaaatggttc attggtatac taaacaaaag gactcaattc ggcaatacaa
     8581 aacaacacta tggataaaat catcaaggat attcccctta caatcacccg cggttgggaa
     8641 aattatgcat tgctggatag cggtaatggg atgaaactgg aacgttatgg aagaatcctc
     8701 ctttcccgtc ctgaacctga agcattctgg acgaaaagct tgccagaaca ggagtggaaa
     8761 aaggcggatg ccgtttactt ctccccttcc caagatgaaa acggtacctg gaagttcaac
     8821 aagcaattcc caaatccttg ggacatccgt gtgggaaacg tgcgctgcaa attagagctt
     8881 accagttccc gccatatcgg gctttttccc gagcaatttc cacaatggca atggctgcac
     8941 gctcatctaa caaacagccc tcaccccttg aagatgctca acctgtttgg atacaccggc
     9001 atggcaaccc tctttgcagc ccaggcagga gcgcaggtga cgcacgtgga tgcctcgaaa
     9061 aaagcggtca gttgggcaaa gaccaatcaa gaaatctcca atttgcaaga aaagcccatc
     9121 cgctggatcg tggatgacgc tctgaaattt atcaaacgcg aggcacgccg gcaaaatttt
     9181 tatgatgcca tcgtgctaga tccgcccaaa ttcgggcgcg gtccaaatgg cgaggtatgg
     9241 gaattctaca agtatttacc caatctgctg caaggctgtg aagagattct cagcccccaa
     9301 ccttcttttc tcttgctgac cgcatatgca gtgaaagctt ctgctctcac cctgctgcgc
     9361 gccctgcaag aacaaatgca agcgcacgga ggcagcatta ccgcaggtga actggtgcag
     9421 caagaggcaa gcagtcaaag atatttatcg cgagccatct atgctctttg ggaaaaataa
     9481 tctttgcgga gaaaaatatg gatccgatct tacacaccta ttctattatt gccagagatg
     9541 aaaggagcgg acaattcggc gcagctgtgc aatcacactg gttcaacgtc ggttcgctct
     9601 gcccctgggt cgaagcgggt gtgggagctg tggctacgca atcgtcggta aaggtcagct
     9661 atggtccaca ggggctgaag ctgatgcgtg aagggaaaag cgcaccagaa gcactgcaaa
     9721 agttactggc aagagatgaa gatagagaaa atcgtcaggt tgccatggtg gatgcgcttg
     9781 gaaatgtggc agtgcacacc ggaacccgct gtatcgccca agcagggcat gtagctggcg
     9841 gtggtttttc tgtacaagcc aatatgatga aaactgatac ggtctgggac gcaatggctg
     9901 aagcatttca aaaaacaaag ggggatctgg cagaacgcat gctggcttcc ctacaggctg
     9961 ctcaagccga aggcggtgat atccgcggca agcagagcgc tgccatgctc gtggctgacg
    10021 gcgttcgcag cgaagagcct tacgaacaca tcctccttga cctgcgtgtg gatgatcacc
    10081 ccgagcctct ggtggaactg gaaaggcttc ttcacatcca gcgtgcctat gagcatatga
    10141 acaaagggga tgactttttg acaaaaggtg aaatggtcga agcgatgaag gagtacagcc
    10201 tgggtgccaa catggcgcct gccattgaag aactcccctt ctggcaagcc gttaccatgg
    10261 cagccaatgg gtatttgtca gaagcgctcc ccatctttca ttcggttttc aagaccaata
    10321 aagattgggc tgaaacacta cggcgtttgc cagccgccgg attcttccca gacgacaaag
    10381 aaatgctgga tacaatcctg caggaactga aaagataaaa ggaggaaata tgcagcagta
    10441 cagagtggat cccgaaaaac cgattaagat caagaaactg gatgccgatg atctgggcga
    10501 atgggaagga aaaaagaaag ccggtcaaaa agcattcgca gatctgcaaa aggaactagc
    10561 aaaacttcag gaagtgctct acgccgaaca caaacacaag gtcctcatta ttctgcaagc
    10621 catggacacc ggcgggaaag atggcaccat ccgctcgctg ttgaaggaag tcaatccgca
    10681 gggtgtgaaa atagccagct tcaaagtccc cactccagtt gaacttgccc atgattacct
    10741 gtggcgagtg cattctatgg ttcccgcgca gggtgaaatg gtgctcttca accgatcaca
    10801 ttacgaagat gtgctggtag tgcgagtgca cgaactggtg tcaaaagaag tttggaaaaa
    10861 acgctttgaa cagatcaacg cttttgaaaa aatgctcaca gaagagggca caaccatact
    10921 caagttttat ttgcatatcg ataaagagga gcaaaagcag cgttttatag aacgattgac
    10981 caatccaaag aagcagtgga aatttaatcc caatgacgtg gaggaacgca agaattggga
    11041 cgtctatcag gaagcctatc aggatgtcct taacaagacc agcacgtcct gggcgccctg
    11101 gtatgccatt cccgccaacc gcaactggta ccgtgacctg tgcgtggcta caatcgtcac
    11161 tgatgcgcta cgatcgttga aagcaaaata tcccgagcca atccctgatc cagagaccta
    11221 cattcaagcg ctggaaaatg aggatatgga aaaataacgc cattgaattc gaaagcgcaa
    11281 cgaagcattt cagtacttta ccgaaagggt ggtttcatcc ttcttctcta tgattgagcc
    11341 gatctcgacg actgggatcc cttccttcgc caaaggcgtt atcattggat tcgcagatgt
    11401 cacatccaaa gtcaccgagt tgatatcatc gattgggatg aaccaactaa agatgaagaa
    11461 tggcagcggc agggcaggtc aaatacacaa accgcgcttt ggagcaaacc agcccgccag
    11521 agcgcactga ctccacgccc ttttacattc agcctttact cgtcaaacca ttctgcgaag
    11581 ctgccttcat aggctctgtt gtctccgctc atatatgcaa tcacagtatc cgagtggccc
    11641 tttgaagtat ctcctccggg agagtgaaaa aatacataat ctttaatcgt tgcctgccac
    11701 tgtgtaccat ctgcggtaat atagttgata atggaatcct tatgtccttc gtttctcttt
    11761 ccctgatgcc aatggaaaaa ggcattcccg ttgtcgatta cttctgcaaa ccacagactg
    11821 ggatcgttcc aagtcttgta cgctatgaat ttacttaccg ttttaacaga acgatctgat
    11881 agaagctggt ttacgaagac aggtttagtg tacttgctca ttatttttcc tccttttatt
    11941 gaacaaatac agaagcttga attccctctg tttaatacag tatatactat tatgaacaat
    12001 tgtacaactg catggatagg ttcaacacat atgaaacagt tgggggcaaa ctgagggtag
    12061 tgccattgaa tatattcttg cagggttaga ttgtccagag aagcatgcag acgtgtgatc
    12121 caaacctgaa aaatgaagcc tgacatgcta tttttcatga tggagcatta ataactacct
    12181 acgatatcaa cagaattcca tcaggtaaga attcgttgtt catctctaga ggtcgagata
    12241 aatttgatcc ttctacacgc ttattagagc catctaataa ttaaacagaa tcgcctaaat
    12301 taatgtagaa caatatatcg gtcgccctgt ctttctgcga tttgaccaat atccactgcg
    12361 gggacgcctt catcatttaa gtattgtgtc attccgaaag cattggaatg ttcaaccgcc
    12421 accaacaacc caccggaggt ttgtgcatcg ttcaggatga tctgctgaat ttccgtcaat
    12481 ctttcagcat agcgcacata gggttttgtg taagcatgat tattgtgact gccacccggg
    12541 atgatccccg ccgctgccag gtcataagta ccttccaaaa ttggaatgga accaaaatcc
    12601 aataccgcag tcaggttagc cgctttcatc attcccagta aatgtcccag caacccgaaa
    12661 ccggtcacat cggtacaggc atgggcatgg aattgctgca gcgctgctcc ggctttttga
    12721 ttgagcagcg ccatagtttg tatcaattgg tccgtttgtt ctttttccag taatccctgt
    12781 ttcaaagcgg tggtcagaat gcctgtgccg atcggtttgg tcagcagcag atggtcgccc
    12841 gcttgtgctt tagcattggt gattagtttt tcaggatgca ccacaccggt aaccgccatg
    12901 ccatacttcg gttccgtatc atccaccgta tgtcctccga tgatggctat gcccgcctcg
    12961 ttagcttttt cctgtgcgcc gcgcagtatt tccctcagaa cagaaactgg caatctgtta
    13021 ctgggaaaac ccacgatgtt caaagcgaac aaaggcgtgg cgcccatggc gtaaatatca
    13081 ctcaatgaat tagccgctga aatggctccg aaaagatagg ggtcatccac aatgggagtg
    13141 aagaaatcca ctgtctgtac aatcgccgtt tcagagttga tccgataaac cgcagcgtca
    13201 tcgccggtct ctgttcccac cagaatgcgt ttatcctttg atggcttgat ctccgccaga
    13261 atctcttcta acaactgcgg gcgcaactta caggcacaac ctaagccatg cgtgtaatgg
    13321 gtcagcttga tcgcttccgt ggagcctgac ttgaagacca cctgctcctg tgcttgcaag
    13381 ttgccgatca cgcgcttgag ctcttccaac gcaaaatcga tctcctcttg ggtcgtattg
    13441 cgcccaacgg aaaggcgaat agttcccatg gcgtactcca caggaatgcc catagctgca
    13501 atgaccggcg agacatccac ctggtcggca tggcaggcag ccccagcaga agctgccacc
    13561 tgatctagtt cagccagaat ggtgttcgct tctaagccgt agaaactcac attggttgtg
    13621 ttaggaagac gtttttcagg gtgcccgttg aatcttacgt tcgagaaggc tgctcgtaat
    13681 ccgttttcca gatgggtgcg catggcaagc atatgcgctt gatattgtgg gagattctct
    13741 ttgatcagtt cacaggcttg ccccaacccg gcgatctcga taacattctc ggtgccagcg
    13801 cgcttgttca tctcgtgctc cgcaccatgc atgatcttct ctacccgcac tccgcgtctg
    13861 ataaacaagg cgccgatccc tttgggagca tacaatttgt gcccggcaat ggaaagcaga
    13921 tcgacaccca actcacccac attcacagcg atcttgccca ccgattgggc agcatcggta
    13981 tgcataagca ccccgtgcgc gtgagcgatc attgcaattt cacgcaccgg ttcaacggtg
    14041 cccacctcat tattggcgtg catgatgctg atcagaatgg tatcgggggt gatagaactt
    14101 tcaacatcat gcgggttcac caaaccaaat tcatccaccg gaaaaatggt gacctggaaa
    14161 ccctgttttt gcaaatacgc caccacatcc agcaccgccg gatgttccac cgcgcaggtg
    14221 atgatatggt tgcctttatc acggtaggca tatgcgaccc ctttgagcgc caggttgttc
    14281 gcctcagaac ccccgctggt aaaaacgatc tcatccggct ggcagcccag catctccgct
    14341 acctggcggc gcgccttctc cacacctttt tttgcagcgc tgccataggc atggctgctg
    14401 gatggattgc caaaattttc gtaaatgaat gggcgcatgg cttctgcgac ctgcggagcg
    14461 ataggagtgg tcgcgttata gtccagataa accggttttt gcatgttcca cctcaaaaaa
    14521 cttccagcgt tcctcgcttc ttggcagtca cctcaaacca gcggaacaac gagtaaccca
    14581 gcaaggcaaa gaacaaaccg atacccacct cttccaccag caaaggaaga acttcaccca
    14641 gcgcatgccc gccgatgatc aagcgggctg aggcaatccc gcgggtgaga ggcagataat
    14701 tggaaacgat ttgcatccac tttggcagag atgtcaattc cagattggcg ccggaaaata
    14761 ccagcaaaac aaaatacacc gtgttattga cgaacatcac attgcgcgta atcaaaccga
    14821 tgcagcccat cagcaacccc agacccgccg tgctgaaggt ggtcaccaag atcgtgagca
    14881 gcagtgccca gggattggtg gcagaataat cgatgtggaa aagcagccat ccccagaaca
    14941 gaccagttat cacaccgatc ataccattca aaatatgcat gaacgctcta cccaagaaaa
    15001 ccgtcatgcg gttggcagga gcgccaaaga gatagggcag cgtaccatcc cagcggtcgc
    15061 caccgatgct catggtaacc ccatagatac cgctcatggc ggcgttcatt aaggcattcc
    15121 caatcacata gaaatacgct ctgcccgcgc cctgggcata ggtgccgatc aacacaaaaa
    15181 agagcatctg ggtgagaggc gaaaaaatca aggtagccag ataggtggag gggcttaacc
    15241 agcggaacaa acctacatat gataggtaag ccccctcaaa aaagatcttg acgttcagtt
    15301 ttattttttc catagtgatt attgcatgtt taacgttgca tcttctctgg ctttgcgcag
    15361 gatagatttg aaaaggaact tggaaatgat tgcatatatg agactaaaga caaccagcat
    15421 tgcccagcaa aagtagattt cggcatcgct gcccgttcca ccggacgccg cgtgcaaagc
    15481 tctggctgcc cagtagggag tgagaatata gctgaaaggc gtggtccaca tgggcagcag
    15541 tgcaataggg aacaggaatc cggaaaaaat atacaccacg aattccagac cattctgaaa
    15601 acgctgcaca tcagggttca agataaacag cggtccgatg atcaatccga aggcaatgaa
    15661 agctacaacg gtaagaacga tcgaaccagc gaaaagccac ggcttggcaa tgttgagagg
    15721 caaacccatc agcagagaaa tgaaggcata acagcaaacg atcg
//
LOCUS       DAOSDS010000048        15122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1572_length_15122_cov_4.108383, whole genome shotgun sequence.
ACCESSION   DAOSDS010000048 DAOSDS010000000
VERSION     DAOSDS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15122
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1572_length_15122_cov_4.108383"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLL88_06905"
     CDS             complement(<1..582)
                     /locus_tag="PLL88_06905"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ01335.1"
                     /translation="MKADALWIVEPNKMEIRPYEVEEPKYDEVMFKTMAGGVCCWDSY
                     LYQGISAQSPMPYVIGHESVGIVEKVGSGVKNLKPGDKVFCASGSNQMMCKYVTVKSD
                     CVVKLADDTSDWVAAVTEPTCCVVNVLYKTFIEPGDHVVLIGAGYMGTLTLQGLTRGS
                     QAGRITVFEIREDRRELAKAYQPAEILDPESPEG"
     gene            complement(844..1770)
                     /gene="rbsK"
                     /locus_tag="PLL88_06910"
     CDS             complement(844..1770)
                     /gene="rbsK"
                     /locus_tag="PLL88_06910"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOJ01336.1"
                     /translation="MKKIIVLGSINMDLVVGAERAPEGGETLFGSSFQTIPGGKGANQ
                     AVATARLGGEVAMIARVGDDGFGDELITKLVENSIDVQQVRRDASRPTGVALIVVETS
                     GQNRILVVAGANGQVSKADVDDAGTLFDRSDFIVLQLETPLETVAYAISVAKSRHVQT
                     VLNAAPARPLPDDLLRQVDYLLVNESEAEAIAGKSVKSVDSAFEVASALQKKGTGKVI
                     LTLGDKGAVAADGEATWHTPAFAVKAVDTTAAGDAFTGGLVTALQQGFALKDAIIRAS
                     AAGALAACTFGAQPSLPTSDELQAFLAAQKVL"
     gene            complement(1772..2806)
                     /locus_tag="PLL88_06915"
     CDS             complement(1772..2806)
                     /locus_tag="PLL88_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ01337.1"
                     /translation="MANIKDVAKLANVSITTVSHIINQTRYVSDELTDRVKKAMEELD
                     YHPNSLARGLRSGKTKTIGLVIPDISNQFFAEISRKIEDKGFEHGYSVILCNTDDDPQ
                     KEKAYIDVLLAKKVEGIIFISTVVESNSLEKPLEFGIPIVVVDRDIKDINSDIVLVDN
                     SVGGYEATKYLIELGHRRIGCIGGPSPITPSAQRVAGYKQALQEAHIPVDTDLIISGD
                     FRYESGNKAMRALLSLPQPPTAVFASNDMMALGAIQAVNDKGMRIPDDISVIGFDNIP
                     FSQTISPTLTTMAQPIHEMAGLVVDLLIEKIKLQRQRGRIEESKPTYKRTVLETKLVK
                     RNSCREIGKR"
     gene            complement(3082..3246)
                     /locus_tag="PLL88_06920"
     CDS             complement(3082..3246)
                     /locus_tag="PLL88_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01338.1"
                     /translation="MPVTSILFFIYIAAIAISAVGIVKKIQVLKIAGAILFFIGICAT
                     ALLVLAVRNM"
     gene            complement(3311..4000)
                     /locus_tag="PLL88_06925"
     CDS             complement(3311..4000)
                     /locus_tag="PLL88_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOJ01339.1"
                     /translation="MIVGGFQKFSLMDYPGKTCAIIFTRGCTFRCRYCHNPELVIPEQ
                     YASRIPLRTIFEFLETRRGKLDAVEITGGEPTQHADLIVVIRSIKDMGFLVKLDSNGS
                     RPEVLKEIINQKLVDYLAMDIKAPLGSYSRIMGWQVPASALRQSIHLIMNSGVDYEFR
                     TTLIKSLISRNDLREIAKTIHGAKKYFLQKFIPLNLVDNHCAHEVSYSDKELKEFAQE
                     LMQYVEFCGVR"
     gene            complement(4009..6093)
                     /locus_tag="PLL88_06930"
     CDS             complement(4009..6093)
                     /locus_tag="PLL88_06930"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOJ01340.1"
                     /translation="MKITQIKKRNGKIIAFEQEKITVAIGKAGKETGEFGFSQAQKLS
                     QQVIEKLQQATTDIPTVEQVQDIVENILLGSCYKKTAKAYILYRNQHKQLRDIANAVN
                     NGLIDDYLNKSDWQVRENSNMSYSLQGLHNYISSEVSKNYWLHKIYPEPIRMAHTTGD
                     FHIHDLNILSVYCVGWDLFDLLLEGFTGVAGKISSKPPKHFRSALGQVVNFFYTLQGE
                     AAGAQAFSNFDTLLAPFIRHDKLGYREVKQALQEFLFNVNVPTRVGFQTPFTNVTLDL
                     AVPQYYADQPVVIGGRPQKETYGEFQEEMALFNKAFLEVMAEGDASGRVFTFPIPTYN
                     MTKDFDWDSEICKLLWRVTGKYGLPYFSNFINSAMNPEDARSMCCRLRIDNRQLEKRG
                     GGLFGANPLTGSIGVVTINLPRIGYEVKTQGQFLARLKELMVLAKDSLEIKRKVLEKF
                     TEANLYPYTRFYLRGIKARHECYWKNHFSTIGIVGMNEALLNLFGEGIATDKGRKFAE
                     EVLDFMRDTLLMFQMETGNNYNLEATPAEGTSYRLALCDQKRFRDSNFANGKGKEPAS
                     PFYTNSTHLPVNYTNDLFEALDLQDALQTRYTGGTVVHLYLGERIEDGEGVKNLVRKV
                     CNSYHLPYFTISPTFSVCPNHGYLAGEVHVCDQCGTQCEIYSRVVGYLRPIDQWNDGK
                     QAEFHIRKTYGV"
     gene            complement(6359..6559)
                     /locus_tag="PLL88_06935"
     CDS             complement(6359..6559)
                     /locus_tag="PLL88_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ01341.1"
                     /translation="MKNEVKRLRKEKGLRQEDVANLVGVTRQTIIAVENDKYNPTLEL
                     AMRLAHLLGVTVEDIFFLDDDQ"
     gene            complement(6549..6791)
                     /locus_tag="PLL88_06940"
     CDS             complement(6549..6791)
                     /locus_tag="PLL88_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01342.1"
                     /translation="MMQRKKWKADEMEMAINFRSIRVAYVFSIGTLLGYCVYDFALHK
                     ELPFVPFIVVCGQLFLFYFSKLWLTKKMTKGNQDEE"
     gene            complement(7068..7139)
                     /locus_tag="PLL88_06945"
     tRNA            complement(7068..7139)
                     /locus_tag="PLL88_06945"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7105..7107),aa:His,seq:gtg)
     gene            complement(7158..8441)
                     /gene="hisS"
                     /locus_tag="PLL88_06950"
     CDS             complement(7158..8441)
                     /gene="hisS"
                     /locus_tag="PLL88_06950"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOJ01343.1"
                     /translation="MKNIIPSVKGTREFYPEDMVLRQWLYKLIREVSESFGYQEYDGP
                     FLETLELYAAKSGEELVKEQSFVFQDRGGNEITLRPELTLSLARMVAQRQNALAFPLR
                     WWSFGPFWRYEKPQKGRAREFFQWNADLIGVDSTQADAEVIALCAAFFKKAGLTNTEV
                     RIYINDRRLMESELIKLGFPLEQHKLIFKLIDRKDKLTERDWDAYVLEAGLSSAQLEA
                     LRKLINNQELWHASETLTTVFNILGLYGLSDYVGYDPRIIRGLDYYTGVVFEAKDLGE
                     NHRAILGGGHYANLVADVGGESLPGVGFAMGDVVFPLVLESHGKLPQLTTMPASILVT
                     VFDETSLPTSIALCNRLRAIDIPVVVYPEVDKLGKQFKYADRCGFRGALVVGPDELQK
                     GEVVLKDLASREQVAFSNDALEKKIVEWLAAKNGS"
     gene            8517..8882
                     /locus_tag="PLL88_06955"
     CDS             8517..8882
                     /locus_tag="PLL88_06955"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOJ01344.1"
                     /translation="MKISELINQTQGKLLTKESSLDPDEIINGGCSADLMSDVLAYIQ
                     PHAVLLTGLVNPQVVRTAQMSDVAAIVFVRGKIPPEETIQLAEEVPIPLISSPLGMYE
                     LSGRLYQMGLPSLENAAKE"
     gene            8890..9903
                     /locus_tag="PLL88_06960"
     CDS             8890..9903
                     /locus_tag="PLL88_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOJ01345.1"
                     /translation="MRDLNFSDQPPRFKGKVITDQLAGSITRVEELAYIIRIEEVMTK
                     TLITIPPDASMQVVADVMREKHISGVLITKNEDLVGLVSTEDLIKCLINGELSFSVSK
                     YMSTELITLNSFDFLIEALKTFTRTGLGRLPVLDENKKLIGIITKGDITYGFLKALEH
                     DYHEEEVRRYRASHLFEDIESDRTSLILRYVIKHYDFLHGGAASSNIKKALLRLGANP
                     QLARRVGIAVYEAEMNLIIHTHHGGSIHVEIEPHQISIYAWDDGPGIADTAEAIKPGY
                     STASEEIRELGFGAGMGLYNISRCVDEMKLESELGKGTQLTMKVFLKEDESVGEGYQT
                     EKE"
     gene            9907..10248
                     /locus_tag="PLL88_06965"
     CDS             9907..10248
                     /locus_tag="PLL88_06965"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOJ01346.1"
                     /translation="MTLEQIIQSLDLEVLTAPEEASTITPTSGYISDMLSCVMTGAKN
                     QSIWVTLQSHNNIVAVACLLDVAAVIITEGAKPDDETIQKAKEEGVTLLSTPHNSFHV
                     VGKLWEMGIRD"
     gene            10286..11026
                     /locus_tag="PLL88_06970"
     CDS             10286..11026
                     /locus_tag="PLL88_06970"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOJ01347.1"
                     /translation="MKTYRAELHIHSVLSPCAAVEMIPPIIVSEALRHGIDLIAITDH
                     NHTANIEAVQKAASGTGLVVLPGMELQTKEEVHSLCLFDTLEQSQAFQKLVDAHLPAI
                     KNNPDFFGEQFVVDASGDYIRSEEQLLIVSCGLSLKEAYQAVADLAGLLIPAHVDRMK
                     FGLLPVLGLVPTDIPLTALEISRHITPSQAVQQYPQLKNFSLLQGGDVHHPDEYLGAN
                     LVSIEKPTIRELKLALQGADGRKVAFLA"
     gene            11134..11667
                     /locus_tag="PLL88_06975"
     CDS             11134..11667
                     /locus_tag="PLL88_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOJ01348.1"
                     /translation="MMETEKTNPVLFNPLNDEKAKAEIDAILEKNKDKPGSVMVILNE
                     LQNAVGFVSEDLQQYVANYLRTPVSKIHGVISFYSFFTTQPRGKHTIKICLGTACYVG
                     GAEQIIEKVKQLIGIGPGETNADWTITVEECRCVGACSQAPVVVIDDEIHGRVKPSTI
                     AKLLRTITVEEKTASVE"
     gene            11671..12234
                     /locus_tag="PLL88_06980"
     CDS             11671..12234
                     /locus_tag="PLL88_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ01349.1"
                     /translation="MKELSLHILDIAENSVSAKAKTISIEVEEDSQKNLLTIIITDDG
                     KGMDAEMLVRVTDPFITTRTTRKVGLGIPFLKEVAEACNGHFLITSTPGTGTRVEATF
                     QRDHIDRMPMGSIQTTFLDLLVGYPDVHWKFKYTMDGEEFFLDSEPIQQALGGVPYSD
                     PSVLKYLKHEINGGIQKIQNVHKQEMP"
     gene            12231..12599
                     /locus_tag="PLL88_06985"
     CDS             12231..12599
                     /locus_tag="PLL88_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOJ01350.1"
                     /translation="MNKIKSLDDLKRVREEALEKRSKASVTGKAHVIVGMGTCGIAAG
                     ARETMKAVLDYIESEKMEGILVTQTGCIGLCEQEPILQVERGGEEKVTYGKVNPDVAK
                     RILKEHVAEGKIVKDFVVNL"
     gene            12631..14412
                     /locus_tag="PLL88_06990"
     CDS             12631..14412
                     /locus_tag="PLL88_06990"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOJ01351.1"
                     /translation="MAYFRSHVLFSTDPTILNQGGSEVKETLLKEIGKHNLTDEVKVI
                     DTPRIGNGKQGPEIMVYPEGYHYIGLTAADIPFLVEEQFLKGRVVEKFLAPAPKEKAQ
                     ELRAPTPKEVRVVLRNCGKINPENIEDYIAEDGYSALAKVLSEYTPDDVLAIIKTSGL
                     RGRGGAGFPAWLKWDLTKKATGSPKYLICNADEGDPGAFMNRSVLESDPHSVIEGMVI
                     AAYTIGATQGFVYCRAEYPIAVETLNIAIEQARNYGLLGNNIFGTDFCFDMEVRKGAG
                     AFVCGEETALISSIEGKRGEPYPKPPFPATQGVFGKPTNINNVETYANIPQIIFRGAE
                     WFASMGTEKSKGTKTFALAGDVKQTGLIEVPFGITLREIVYDVGGGIKDDKGFKAIQT
                     GGPMGGCLPAPFLDLPVDYESLTSAGSIMGSGGMVVMDEDTCMVDIARFFMEFTQAES
                     CGKCVPCRVGTKRILEILTRICEGKGKDGDIEMLEMLCSQTIENSLCGLGKGAANPIV
                     STLKHFRNEYEAHIYDKKCPAKTCKALIHYEIDAENCVGCTMCARNCPVNAISGSPKQ
                     THVIDPDICIRCGICKQVCNYEAVVVT"
     gene            14436..14912
                     /gene="nuoE"
                     /locus_tag="PLL88_06995"
     CDS             14436..14912
                     /gene="nuoE"
                     /locus_tag="PLL88_06995"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOJ01352.1"
                     /translation="MTGQIEIADMTESVQNALELYERKEEELIPILSKVNRELGFLPQ
                     KALELISEELKVPKSIVKSVASFYHMLSTKPLGRHVIKFCENAPCHVAGGREVWAALK
                     EATDLDAGETSSDGKWSLLTTSCLGLCAVGPVMVIDGEVYGNLTVEKVPEILGRYE"
     gene            14924..>15122
                     /locus_tag="PLL88_07000"
     CDS             14924..>15122
                     /locus_tag="PLL88_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOJ01353.1"
                     /translation="MKTYRSMVLISNDPHSMLQGAQEIFDCVKEEVKKYQLEDEISIA
                     MASDISRTDISPVVIVYPEAVA"
BASE COUNT         4015 a         3818 c         3474 g         3815 t
ORIGIN      
        1 tccctctggg ctttcggggt ccagaatttc agccggctga tatgctttcg ccagttctcg
       61 tcggtcctcc ctgatctcaa aaacggtgat ccgacccgcc tgcgaaccgc gcgtcaaccc
      121 ttgcagggtc agcgttccca tgtaccctgc cccaatcaaa accacatgat caccgggctc
      181 gatgaaggtc ttgtagagga cgtttaccac acagcaggta ggttcagtga cagcagccac
      241 ccaatctgag gtatcatccg ccagcttcac cacacaatcg gatttcacgg taacgtattt
      301 gcacatcatt tgattggatc cggaagcgca aaaaactttg tcgccaggtt tgagattttt
      361 cactccgctg cccacctttt cgacgatccc caccgattca tgcccgatca cgtaaggcat
      421 ggggctttga gcgctaattc cctggtagag ataagaatcc cagcagcata caccccctgc
      481 catggtcttg aacattacct catcatattt cggttcttcc acttcataag gacgaatctc
      541 cattttgttg ggttcaacaa tccaaagtgc atctgctttc ataatttgtt ctttatcctc
      601 catttcgttt tttatgcaac cgataaaaag caaatgtttg cgcaatcatt tgcttatcat
      661 actcattttt aatggggatg tcaagttgct gcatcctaaa aagccaggaa aagtacgatc
      721 acaacgaaca agaatgcgat ttcattgggg agatttggta ataaagaagg tactgtggtc
      781 taacccgagc caatttaact cctaacaaat attgataaac aacataatcc ctattccagg
      841 atctcatagt accttttgcg cagccaggaa agcttgcagt tcatcagatg tcggcagtga
      901 cggttgcgct ccaaatgtac aggcagccag tgccccggct gcgctggcgc gaattatagc
      961 atctttaagg gcaaatccct gctgaagagc ggtcaccaaa ccgccggtaa atgcatcgcc
     1021 ggcagccgta gtatccacag ctttcactgc gaacgccggg gtatgccagg tagcttcccc
     1081 atccgcagcc accgcccctt tatcacccag cgtcaggatc acctttccag tgcccttctt
     1141 ttgcagagcg ctggcgactt cgaaagccga atccacgctc ttgaccgact ttccagcgat
     1201 ggcttccgct tccgattcgt tgacaagcag ataatcgacc tgcctgagca gatcatcagg
     1261 gagcggacgt gccggagccg cgttcaacac agtttgcaca tggcgggatt tcgccacgct
     1321 gatggcgtac gcaacagttt ctaagggagt ttccaactgc aatacgatga agtcactgcg
     1381 atcgaataaa gtgccggcat catccacatc tgctttgctc acctgcccat tcgccccggc
     1441 aaccaccagg atacggttct gccctgacgt ttctacaaca atcaatgcca cccccgtggg
     1501 gcggctcgca tctcgcctta cctgttgcac atcgatcgaa ttctcaacca gcttggtgat
     1561 cagttcatcg ccgaatccgt catcccccac cctggcaatc atggcaacct ctccgcccag
     1621 ccgcgcagtg gcaactgcct gattggcacc tttgccaccc gggattgttt gaaaactcga
     1681 cccaaataag gtttcacccc cttccggcgc ccgttcagcg cccaccacca ggtccatatt
     1741 gatgcttccc agaacgatga tcttcttcat gctatctttt tcctatctct ctgcaggaat
     1801 tccttttcac aagttttgtt tccaagacag tgcgtttata cgtgggcttc gactcttcaa
     1861 tcctgcccct ctgtctctga agcttgatct tttcaatgag caggtcaacc accagacctg
     1921 ccatttcatg gattggttgt gccatcgtgg tcagggttgg tgatatggtt tgagaaaatg
     1981 ggatattgtc aaagccaatc acagaaatat catccggaat tctcattccc ttatcgttca
     2041 cggcttgaat agcgcccaaa gccatcatat cattgctggc aaacacggca gtgggtggct
     2101 gcggcagcga taacagagca cgcatagcct tattgccgct ctcataccgg aaatctccag
     2161 aaatgatcag gtcggtatcc accgggatat gcgcttcttg cagcgcctgc ttgtaaccag
     2221 ccactcgctg tgcgctgggc gtgatgggcg aaggtccacc gatgcaaccg atgcggcggt
     2281 gacccagctc gatcagatac ttggtggctt catatccgcc cacgctgttg tcaaccagca
     2341 cgatatcaga attgatatct tttatatcgc gatcaaccac aacaataggg attccgaact
     2401 ccagcggttt ttccaggctg ttcgattcca ccaccgtaga aataaagatg ataccctcta
     2461 ctttcttcgc taagagaaca tcaatataag ccttttcctt ctgtggatca tcatcggtgt
     2521 tgcacaagat gacgctatac ccatgctcaa aacccttgtc ttcgattttt cgcgagattt
     2581 cggcaaagaa ctggttggaa atatcgggga tgaccagccc gatggtcttc gtctttccgc
     2641 tgcgcagacc gcgcgccagc gaattaggat ggtaatccag ttcttccatc gctttcttca
     2701 cgcggtcggt gagctcatcg ctcacataac gggtttgatt gatgatatgg gaaacggttg
     2761 tgatggacac gttagccagt ttggcgacat ccttgatatt tgccataaat gtgtataatc
     2821 cctgtctttg cgcaaacatt tgcgcaaatg tttacaacat ggtacacgct attatttgcg
     2881 ctgtcaagtt attgtataag ttcacccgcc cacagtcaat tggacgatcg gcatcaattc
     2941 ttctggatat cacctggcaa tttcacgctc aaactacccg cgctcttcat caaagaagca
     3001 gccttcaaag ggtgtgttgt ctttgagggt gaaaacggtc ttcccccatc tgaggatagc
     3061 ctgtaagcca tcacctcacg tctacatatt tcttacagcc agaacaagaa gggcagttgc
     3121 acaaatccct atgaagaata aaattgcccc cgctattttc agaacttgta ttttttttac
     3181 gatgccaact gcactgatag cgatcgccgc aatgtagatg aaaaatagaa tagaagtaac
     3241 cggcatgtgt cctccacttt tctatattta tcctggcaca ttctcaggtg caagccttct
     3301 ctggttgatt ttaccgcact ccacaaaatt caacatactg catgagttct tgtgcgaatt
     3361 cttttaactc tttatcgctg taagaaacct catgggcgca gtgattatcg accagattaa
     3421 gagggataaa cttttgtaaa aaatacttct tggcaccatg gatggttttt gctatctctc
     3481 tcaaatcgtt tctactgatc aaggatttga tcagggttgt tcgaaattcg tagtccacgc
     3541 ctgaattcat gatcagatgg atgctctgtc ttaatgcgct tgctggcacc tgccaaccca
     3601 tgattctgga ataactgccc agcggagcct taatgtccat cgccagatag tcaaccagct
     3661 tttgattgat aatctccttc agaacttctg gtctgctgcc attgctatca agtttaacca
     3721 ggaaccccat atctttgatg cttctgatca caacgatcag gtcggcatgc tgggtcggct
     3781 cgccgcccgt gatctccacc gcatcaagct tacccctccg cgtctccagg aattcaaaga
     3841 ttgttctcaa tggaattctt gaagcatact gttcaggaat caccagctcc gggttatggc
     3901 agtagcgaca acggaaggta caccctctgg taaaaatgat ggcgcaagtt ttaccagggt
     3961 aatccattaa cgaaaacttc tgaaatcctc ctactatcac gatcgcggtt aaactccata
     4021 ggtcttccgt atgtgaaact ctgcctgttt cccatcattc cactgatcaa tgggtcgcaa
     4081 atacccaaca acccttgagt aaatttcaca ctgcgttccg cattgatcgc aaacatgcac
     4141 ttccccggca agatagccat ggtttgggca gacgctgaaa gttggagaga tagtgaaata
     4201 cggcaggtga taagagttgc aaaccttcct caccaggttc ttgacaccct cgccatcctc
     4261 aattctttct cccaggtaca aatgcactac cgtgcctccc gtatacctgg tctgcaaggc
     4321 atcttgtaaa tccagcgctt caaacagatc gttggtgtaa ttcacaggca aatgggtaga
     4381 gttggtatag aaaggactgg caggttcttt gcccttacca ttggcaaaat tgctatcgcg
     4441 gaatcgtttt tgatcacaaa gagccaagcg atacgaggtc ccttcagccg gtgttgcttc
     4501 aaggttatag ttattccctg tttccatttg aaacataagg agcgtatccc gcataaaatc
     4561 caacacctct tccgcaaact tcctgccttt atccgtggcg ataccttctc cgaacaggtt
     4621 cagcaaagct tcgttcattc caacgatgcc gatggtggaa aaatgatttt tccaatagca
     4681 ttcgtggcgg gcttttatgc ctctcaaata aaacctggta taggggtaga gatttgcctc
     4741 ggtaaacttt tccaatacct tccgtttgat ctccaaacta tcctttgcca ggaccatcag
     4801 ttccttcagc ctggctaaaa actgcccttg cgtcttcacc tcatacccaa tgcgcggaag
     4861 gttaatggta actaccccaa tagaccctgt taacgggttg gctccaaaaa gcccaccgcc
     4921 ccgcttttcc aattgacggt tgtcaatgcg caaccggcag cacatagagc gggcgtcttc
     4981 cgggttcatg gcggagttga tgaaatttga aaaatacggc aggccatatt tgcccgtcac
     5041 ccgccacagc aacttgcaga tctcactatc ccaatcaaaa tcttttgtca tgttataggt
     5101 cggaatggga aaggtaaaca cccgaccgct ggcatctccc tccgccatga cctccaaaaa
     5161 agctttatta aacagtgcca tctcttcctg gaattccccg taggtttcct tttggggtct
     5221 tccgccgata acgacaggtt ggtcagcgta atattgggga acggctaaat cgagagtgac
     5281 attggtaaag ggggtctgaa acccaacacg agtgggaaca ttgacattga acaaaaattc
     5341 ctgcaatgcc tgctttacct ctctataacc aagcttgtcg tgccggataa agggggcaag
     5401 aagggtatcg aagttgctaa atgcctgcgc tccggcagcc tctccttgaa gagtatagaa
     5461 aaagttgacg acctgtccca gggctgagcg aaaatgtttg gggggtttac tggaaatctt
     5521 tccagccact cccgtaaatc cttccagcaa caaatcgaac aaatcccacc ccacgcaata
     5581 aacggagaga atgttcaggt cgtggatgtg gaagtcgcct gtagtgtgcg ccatgcggat
     5641 cggttcagga tagatcttgt gcaaccagta gttcttgctc acctcgctgg aaatatagtt
     5701 atgtaaaccc tgcaaggaat aggacatgtt gctgttttct ctaacctgcc agtcagattt
     5761 gttgaggtaa tcgtcaatca agccgttatt gacagcgttg gctatatcgc gaagctgctt
     5821 atgctggttg cgatataaaa tatacgcctt agcggttttt ttgtagcagg aacccagcag
     5881 aatattctcg acaatgtcct ggacttgttc caccgtaggg atatcggttg tggcttgttg
     5941 taatttttcg ataacttgct gcgaaagttt ttgggcttga gaaaaaccga actcgcccgt
     6001 ttccttgcca gccttgccaa tggcaacagt aattttctcc tgctcgaaag caataatttt
     6061 gccattccgt tttttgattt gtgtaatttt cattggtgtt tgcatagccc taaaaacaaa
     6121 gaacttggct tttcgagcca agttcttcaa tagtaaagcg gaatggcttg gcgcgagcct
     6181 aaagcaaatc cttgcgaatt tttacacagg atcggactat accgttgcgc gacagtgcca
     6241 gattctcact ggacttcact gtgtaaactg ccttatgaaa acattttaca ataaaccaat
     6301 ttttattaca agagatccac aaagttgcca tgcattaaac gtattggttt agcagatctt
     6361 actggtcatc gtcaaggaaa aagatgtctt ccacagtcac acccagcaaa tgagccagtc
     6421 tcatcgccag ttctaaggtg ggattatatt tgtcattttc aacggcaatg atcgtttgcc
     6481 gggtaacccc taccagatta gccacatctt cctgacgaag acccttctcc ttgcgtaaac
     6541 gtttgacctc attcttcatc ctgattgcct tttgtcattt tttttgtcaa ccaaagcttt
     6601 gagaaataaa acaaaaacaa ctgtccgcag accacgataa aagggacaaa cgggagctct
     6661 ttgtggagag caaaatcata cacgcaatag cctaaaagcg taccgataga aaaaacgtac
     6721 gcaacacgga ttgatctgaa attaatggcc atctccatct cgtccgcttt ccatttcttt
     6781 ctctgcatca tctacctcct cacagaatgt ataatgttct ttacatttca tcataccctg
     6841 ctattgttga aatgtcaaga ggtttttaca ttttcgtggg tcgttttcaa gcaatggaat
     6901 ataacgaaca gagcgcgcta tttatggaac aaattggata gatatgccgt ttaggctgaa
     6961 gaaaataaaa ataagatgta tgaatataat agggcaatac gtgtagcgaa agacgatccc
     7021 cgatacaaaa aagtaggact tttgctgaga aaagtcctac ccatcagggt ggctgatggg
     7081 ggttgaaccc acaaccactt gatccacagt caagtgctct gccattgagc tacagccacc
     7141 acgtgtgctc gattgtatca tgagccattt ttagcagcaa gccattccac aatcttcttt
     7201 tccagtgcgt cgttggagaa ggcaacctgc tcgcgcgaag cgagatcttt cagcaccacc
     7261 tccccttttt gcaactcgtc cggaccaaca accagcgccc cccgaaagcc gcagcgatca
     7321 gcgtacttga attgtttgcc cagtttatca acctccggat agaccaccac cgggatgtca
     7381 atcgcccgca agcgattgca caacgcgatt gaagtgggca aactagtttc atcaaaaact
     7441 gtaaccagga tactggcggg catggtcgtc aattggggta attttccatg actttccaat
     7501 accagtggaa aaaccacatc gcccattgca aaccccaccc cgggcaggct ttctccgccc
     7561 acatccgcca ccaggttggc gtaatgcccg ccgcccagga tagcgcggtg attttcaccc
     7621 aggtctttcg cctcaaaaac cactcccgtg tagtaatcca atccacgaat gatgcgcgga
     7681 tcataaccga cataatcgct caacccatac aacccaagga tgttgaaaac ggtcgtgagc
     7741 gtctcagacg catgccacaa ctcctgatta ttgatcaatt ttcgcaaagc ctccagttgt
     7801 gcactggata agccggcttc cagcacatag gcatcccaat cgcgctccgt tagtttatcc
     7861 ttgcggtcga tgagtttgaa gatcagctta tgctgttcca acggaaagcc aagctttatc
     7921 aattcacttt ccatgagccg ccggtcattg atgtagatac gcacttcagt gtttgtcaag
     7981 ccagctttct tgaaaaaagc tgcacacagt gcgatcactt ccgcatccgc ctgggtcgaa
     8041 tccaccccaa tcaggtcggc gttccactga aagaattcac gcgcccgccc tttttgcggt
     8101 ttttcataac gccaaaacgg tccaaaagac caccagcgca gcggaaatgc cagcgcgttt
     8161 tggcgctgtg ccaccatgcg cgccaggctc aaggtcaatt caggtcgcag ggtgatctca
     8221 ttccctcctc tatcttgaaa aacaaaggat tgttctttaa ccaattcttc gcccgatttg
     8281 gcagcataca attccaatgt ctccagaaac ggaccgtcat attcctgata cccgaatgat
     8341 tccgaaacct cgcggatcag cttgtacagc cactggcgca aaaccatatc ttcagggtag
     8401 aattcgcgtg tgcctttgac agaagggatg atgtttttca ttttttacct gcatcaatga
     8461 cttatgccaa ggattttaac atataattac gctgaaccta tgatactgtg agatttatga
     8521 aaatatcaga attaattaac caaacccagg gcaagctgct caccaaggaa agcagtttgg
     8581 accctgatga gatcatcaat ggcggctgca gcgccgatct gatgagcgac gtgctggctt
     8641 atattcaacc ccatgcggtc ttgttgaccg gtctggtcaa tccacaggtg gtgcgcacag
     8701 cgcaaatgtc cgacgtggca gccatcgttt tcgtgcgcgg caagatcccc cccgaagaga
     8761 ctattcaact tgccgaagag gtgccgatcc cgttgatcag ttctccgctt ggcatgtatg
     8821 aattatcagg tcgcctgtac cagatgggct tgccaagcct ggagaacgca gctaaagaat
     8881 aaaacctcca tgagagattt aaatttcagc gaccagcctc cccgcttcaa aggaaaagtg
     8941 atcaccgatc agcttgccgg aagcatcacc cgggtggagg aattggcata catcattcgc
     9001 atcgaagaag tgatgaccaa gacactcatc accatcccgc cggatgcgag tatgcaggtc
     9061 gttgcagacg tgatgcgcga aaagcatatc tctggagttc tgataaccaa aaatgaagac
     9121 ctggttggtc tggtcagcac agaagatctc atcaagtgct taatcaatgg agagctttcc
     9181 ttttcggtca gcaagtacat gtcgacggaa ctcatcaccc tgaattcctt cgattttctg
     9241 atcgaagcat tgaaaacctt cacccgcacc gggctcggtc gcttgccggt tttggatgag
     9301 aataagaaac tgatcggcat tatcacaaag ggtgatatca cctatggctt tctcaaagcg
     9361 ttagaacatg attatcatga ggaagaagtg cgccgatatc gcgcatcgca tctgttcgaa
     9421 gatatcgaat cggaccgcac cagtttgatc ttgcgctatg tgatcaagca ctatgatttt
     9481 ctgcacggcg gcgcagcctc cagcaatata aagaaagcct tgctccgcct gggagccaac
     9541 cctcagttgg cgcgtcgggt cggcattgcg gtgtatgagg cagaaatgaa cctcatcatc
     9601 catacccacc atggtggttc tattcatgtc gagatcgaac cccaccagat cagcatttac
     9661 gcctgggacg atggtccggg catcgccgat accgctgaag ccataaaacc gggctattct
     9721 accgcttctg aagagatacg cgaattaggc tttggggctg gcatgggttt gtacaatatt
     9781 tcgcgctgcg tggatgagat gaaattggaa tcggaattgg gaaaaggcac ccagttaacc
     9841 atgaaggttt ttctcaagga agatgaatca gttggcgaag ggtatcaaac agaaaaggag
     9901 taacacatga cacttgagca gatcatacaa tctctggatc tcgaagttct aaccgctccc
     9961 gaagaagcat ccaccatcac tcccaccagc ggctacattt ccgacatgct gagctgcgta
    10021 atgaccggag caaagaatca aagcatttgg gtcacgctcc agtcgcacaa taatatcgtt
    10081 gctgtggctt gtttgctgga tgtagctgcg gtcatcatca ccgaaggcgc caagccggat
    10141 gatgagacca ttcaaaaagc taaagaggag ggcgtgaccc tgctttccac cccacacaat
    10201 tcctttcatg tggtcggaaa attatgggaa atgggcatcc gcgattgaat cgcggatgtt
    10261 ttttatgctt tttaaataga tcacattgaa aacatatcga gccgaacttc atattcatag
    10321 cgtgctttca ccgtgcgctg cggtagagat gatccctcct atcatcgtat cagaagcgct
    10381 acggcacggg attgatctga tcgcaatcac cgatcacaac catacggcga atatcgaagc
    10441 ggtacaaaaa gcagccagcg gcaccggctt ggtcgtactg cccggcatgg agttgcagac
    10501 aaaagaagaa gtgcactcgc tttgcctctt tgacacgtta gaacaaagcc aggcgtttca
    10561 aaaacttgtt gatgcgcatt tgcccgccat caaaaataac cctgacttct ttggcgaaca
    10621 atttgtggtc gatgcgagcg gtgattacat ccgcagtgaa gaacaactct tgatcgtttc
    10681 ctgtggtctt tcgctcaaag aagcttatca agccgtagct gacctggctg gtttgctcat
    10741 cccagcccat gtcgatcgga tgaaatttgg gttattgcca gtgctggggc tggttcccac
    10801 agatatccct ctgaccgcgt tggagatatc gcgccatatc acaccctcgc aagccgtgca
    10861 gcaatacccg cagttgaaga atttctcact gcttcagggg ggagacgttc atcatccgga
    10921 tgaatacctg ggagccaacc ttgtttctat cgaaaaaccg accatccgcg agcttaaatt
    10981 ggctcttcaa ggcgcggatg ggcgaaaagt tgcattttta gcctgatcct ttcccttctt
    11041 ttattcacac tttttccgtt atgatcgttc catagaaata ccatcacaaa catgataaaa
    11101 tcacttggtt taagaaaaaa tagatcggtg agaatgatgg aaactgaaaa aacgaacccc
    11161 gtattattca accccctgaa tgacgaaaaa gctaaagccg agatcgatgc gattctcgag
    11221 aagaacaaag ataagccggg cagcgtcatg gtgatattga acgaattgca gaacgctgtc
    11281 ggcttcgtat cagaagattt acaacaatat gtggcaaatt atttgcgtac accggtcagc
    11341 aagattcacg gtgtcatttc tttctactcc ttctttacca cccaaccgcg cggaaagcac
    11401 accatcaaga tctgcctggg cactgcctgt tatgtgggtg gcgccgaaca gattatcgaa
    11461 aaggtcaaac agcttatcgg catcggtccc ggcgaaacca acgccgattg gaccatcacg
    11521 gtggaagaat gccgctgcgt tggcgcctgc agccaggcac cggtggtggt aatcgacgat
    11581 gaaatacacg ggcgcgtcaa accaagcact attgcaaagc tgctgagaac aattacggtc
    11641 gaagaaaaga cagcgtcagt ggagtgagcg atgaaagaac tttcacttca tatattggat
    11701 attgctgaga acagtgtttc agcaaaagcc aaaacaatat ccattgaagt ggaagaagac
    11761 agccaaaaga atttactcac cataatcatt acagacgatg gaaaaggcat ggatgcggaa
    11821 atgcttgtcc gggtaacaga tccttttata accacgcgca caacgcgcaa agtaggcttg
    11881 ggcattcctt ttttaaagga agttgcggaa gcgtgcaatg ggcatttctt gattacatcc
    11941 accccaggta cagggacccg cgtagaagcc accttccagc gcgatcatat cgatcgcatg
    12001 cctatggggt ccatccaaac tacatttctc gatctcctgg tgggttatcc tgatgtacat
    12061 tggaaattca agtatacaat ggatggagag gaattctttc tggatagcga gcccattcag
    12121 caagcgttag gaggggttcc ctattcggat ccaagcgtat taaagtatct aaagcatgag
    12181 attaatggtg gaattcaaaa aatccaaaat gttcataaac aggagatgcc atgaacaaaa
    12241 ttaaaagtct tgacgatctg aagagagttc gtgaagaagc tcttgaaaag cgctccaaag
    12301 caagcgttac cggaaaagcc catgtgattg tcggcatggg aacctgcggc atcgccgccg
    12361 gagcgcgcga aaccatgaaa gccgtccttg attacattga atctgaaaaa atggaaggca
    12421 tcctggtaac gcaaaccggc tgcatcggtc tctgcgaaca ggagcccatc ctgcaggttg
    12481 agcgaggagg cgaagaaaag gtcacgtatg gaaaggtcaa tcccgacgtg gcgaaacgga
    12541 tccttaaaga gcatgtagct gaaggtaaga tcgtcaaaga tttcgtcgtt aatctttagt
    12601 ttttttgatt ggaggaaaac gttttttact atggcttatt ttcgttcgca tgttctcttc
    12661 agcactgatc ccaccatcct caatcagggt ggttcagagg taaaagaaac actgctgaaa
    12721 gagatcggaa aacataattt gacagatgag gtcaaagtca ttgatacccc tcgcatcgga
    12781 aatggcaaac agggacccga gatcatggtc tacccggagg gctatcatta cattggctta
    12841 accgcagcag atatcccttt cctggtcgaa gaacagttct taaaaggcag agttgttgag
    12901 aagtttctgg ctcctgcgcc caaagaaaaa gcgcaggaat tgagggcacc cacgcccaaa
    12961 gaagtgcgcg tggtattgcg caattgtggc aagatcaacc ccgaaaatat tgaagactat
    13021 atcgctgaag atggttattc agcgttggca aaagtgttat ctgagtacac gcccgatgat
    13081 gtgttggcga tcatcaaaac ttccgggtta cggggtcgtg gtggcgccgg attccccgcc
    13141 tggttgaaat gggatctgac caagaaagcc accggctcac ccaaatattt aatctgcaac
    13201 gccgatgaag gcgaccccgg cgcgttcatg aaccgctcgg tactcgaaag cgatccccat
    13261 tccgtcatcg aagggatggt catcgctgct tatactattg gagccactca gggcttcgtg
    13321 tattgccgtg ccgaataccc gatcgcagta gaaacgttga atattgccat tgaacaagcc
    13381 cgcaattatg gcttgttggg aaacaacatc tttgggaccg atttctgctt cgacatggaa
    13441 gttcgtaaag gtgccggcgc gttcgtgtgc ggtgaagaga ccgccttgat cagttccatc
    13501 gaaggaaaac gcggcgagcc ataccccaaa ccccccttcc cggcaaccca gggtgtcttc
    13561 ggtaaaccga ccaacatcaa taatgttgaa acctatgcca acattccgca gatcatcttc
    13621 cgcggcgcag aatggttcgc atccatggga acagaaaaaa gcaaaggcac caaaacattt
    13681 gccttggctg gtgatgtgaa gcaaaccggt ttgatcgaag tgcctttcgg catcaccttg
    13741 cgcgagatcg tttatgacgt cggtggcggc atcaaggatg acaaagggtt caaagccatc
    13801 cagacaggcg gtcctatggg cggttgtttg cctgcgcctt tcctggatct gccggttgat
    13861 tatgaatctc tcacctcggc tggttccata atgggctctg gcggcatggt ggtcatggac
    13921 gaagatacct gcatggtgga tatcgcccgc ttcttcatgg aattcaccca agcggaaagc
    13981 tgtggcaaat gtgtgccatg ccgtgttgga accaaacgca tcctcgaaat cctgactcga
    14041 atttgcgaag ggaaaggaaa agacggcgat atcgaaatgc tggagatgtt atgcagccag
    14101 accatcgaga acagcctgtg cggtctcggc aaaggcgctg caaatcctat tgtcagcaca
    14161 ttgaagcatt tccgtaacga atatgaagct catatctacg ataagaaatg tccggcaaaa
    14221 acctgcaaag cactcattca ttatgagatc gacgctgaaa attgcgtcgg ttgcaccatg
    14281 tgcgcaagga attgcccggt gaatgcaatt agcggctctc ccaaacaaac gcatgtcatt
    14341 gatccagata tttgcatccg ttgtggtatt tgtaaacagg tctgcaacta cgaggcggtc
    14401 gtagtgacat aatacagtac aacaggaggt tctttatgac aggtcagatt gagattgccg
    14461 atatgaccga atcagttcag aatgcgctgg aactctatga gaggaaagaa gaggaactca
    14521 tcccgatctt atcaaaagtg aatcgggaat tgggcttcct cccccagaaa gcgctggaac
    14581 tgatcagcga agagttgaag gtaccaaaaa gcatcgtgaa atccgttgcc agtttctacc
    14641 acatgctctc caccaaaccc ctgggcaggc atgtgatcaa attctgtgaa aacgccccct
    14701 gccatgtcgc gggagggaga gaagtatggg cagccctgaa agaggcaacc gatctcgatg
    14761 ctggagaaac cagctccgac ggaaaatggt cccttctcac aacgagctgc ctgggtcttt
    14821 gtgcggtagg tccggtaatg gtgatcgacg gggaagttta tggcaatttg actgtcgaaa
    14881 aagtgcctga gatcttggga cggtatgaat aggagatggc atcatgaaaa cttatcgatc
    14941 gatggtgttg atttctaacg acccccacag catgcttcag ggagcgcaag aaatctttga
    15001 ttgcgttaag gaagaagtga aaaagtatca attggaagac gaaatttcca ttgcaatggc
    15061 atccgatatc agtcgaaccg atatttctcc tgttgtgatt gtgtatcccg aagcagttgc
    15121 tt
//
LOCUS       DAOSDS010000049        15097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1576_length_15097_cov_4.694987, whole genome shotgun sequence.
ACCESSION   DAOSDS010000049 DAOSDS010000000
VERSION     DAOSDS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15097
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1576_length_15097_cov_4.694987"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PLL88_07005"
     CDS             complement(<1..923)
                     /locus_tag="PLL88_07005"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOJ01354.1"
                     /translation="MKLLFLDLIQRPALLVDLQKADANIASMSAKAVRNKVAFRPHFK
                     THQSSRIAELFRKRGVSAITVSSVAMARYFADAGWQDILIAFPLNWREMDALNALAER
                     VRLGVLVDCQESVEYLAFHVRHAVDVWIKIDTGLHRSGIWWQDQTHIETLTRVIMRSA
                     RLNFRGILTHAGQAYHAKSPQNVKELAVTSQQAMLAVKDGLLAAGFEDVLVSIGDTPG
                     CRLIDDFSGVDEIRPGNFIFFDLEQWQLGACSQEEIAVAMACPIVSKNMTRNELVVYG
                     GAIHFSKETMPYQGEPIYGLVAGEFNQPFGML"
     gene            953..1399
                     /locus_tag="PLL88_07010"
     CDS             953..1399
                     /locus_tag="PLL88_07010"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ01355.1"
                     /translation="MDNDTIARIFLRIARLLEIKGESNFKITAYERAADSIRNAPEDV
                     SAMDEQTLQTIPGIGEAIAKKITEFNQTGELNFLKKLETEIPPSLLEWLELPGVGPKT
                     TTLIWKTLGITTLPELLAAAKSGKLHALPGIGEKLETKILQAIEEQ"
     gene            complement(1419..3752)
                     /locus_tag="PLL88_07015"
     CDS             complement(1419..3752)
                     /locus_tag="PLL88_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01356.1"
                     /translation="MGINFHTYPLRIADGNVSATAKGSLVVASSFKSHRHRKGEVLAL
                     QLDFSEPALIAEEEIKEIIAGAGKKFFETPGSVTNALQTAVASVNTALFERNLAPESV
                     EPARATGCFIVSHNGYLFTAQTGASHIFLIGADSFVHLQNQDDGERGLGSARRFLLNF
                     QQLPLTSGDLIMLSTRAPLTWDENHLKNSQQLSMEQVYRRLLDQVNIDLNAMVIKCKE
                     GKGLVQEEQWSVETPALSSEAGNVEQIEEQAAVQEKTEQDYIPAKVEEQVSPTVQHRT
                     SKADHDLTLGQFFPEMEEETLNVEPGRPERIDFEKRKTPLEGETRKGKKSEYVKSRIP
                     SKQGTEKKRSSNALQTLFLRISNWFIADSTPLESNAPTRLLSTAVIIVPLVLITMAVL
                     VYVYSGRREQHQVFLDEAQSYITQATTIEDAAQQREYWTRAYETVLKAMEFGDSDLAN
                     TLLVQTQTVIDDMDLVTRLDFRPATTSQFSQNVVLTKIKSNESGIYLLDATAGSVYRA
                     IANSRGFYEIDSTFRCSPGTYGVITLGKIVDFTTLPTNTRSFEILAVDAGGNVLYCLS
                     GSDPVPSSLIPPASEWGKIGAISLDEYTLYVMDVEKDGIWSYSGSDFEIAAMAGIVFN
                     SHPVDFLGAEEVDLGGAMDLIVNKEDVFILHQDSHMTTCQYNPYREGNATECQDPTAY
                     GDSRIGYEKNPLVYFDAQFNAIQETTYPTSAFYILDSANRAVLQYSYQLNLERTLKPQ
                     PSKTYPLPETEMSGVGVSMGKELFLAFGNQLYVGKLP"
     gene            3867..6209
                     /locus_tag="PLL88_07020"
     CDS             3867..6209
                     /locus_tag="PLL88_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ01357.1"
                     /translation="MVNRKNRELYLGKLFDLSDKKLLTDQPLMYDLDDLTTHAIVTGM
                     TGSGKTGLCIGLLEEAAMQQIPAIIIDPKGDLTNLFLHFPELAPADFEPWINPDLARG
                     EGKTIPEMAAITAENWQKGLKEWEIGKEDILKLQSSVERAIYTPGSSRGIPVNVMTSF
                     EAPMMDWEENEDVLREMISSTVSALLTLVGFKDIDPLRSREHILIANIIEDSWKKNNS
                     LDLTELILLIQEPPITKLGAFPMDKFFPKKERDELALRLNNFLASPSFQSWIEGQSLD
                     VAQLLFAPSGKPRHSVFYLAHLNDTERMFFVTLLFAAIDAWMRRQSGTTGIRVLLYFD
                     EILGYLPPVANPPSKPLIIRLLKQARAFGLGLILTTQNPIDVDYKALSNAGTWIIGRL
                     QTDQDKQRLLDGLESAAGGLSRSVLDKTISSLGKRVFMLQNVNEKQPIIFQTRWAIDY
                     LTGPLTIQQIPALNRLAGAQMPEPAIGGVEKIRSETYSTTPSASLSSTSAAIPYIRKG
                     SGASGKNASLLAQAEVNFYSRTPAINASHVITVVIPNPPQRALDWEKSRIENLTKDSF
                     IQATDLKTGQKDVSEWLLDSKWWMERQKEFEQWVYESDSLSVFRNKDTGLQSQAGEDR
                     ESFVQRLTTATQEKLKTEQAVLERSFQSKLLSAQNKVERQQLRVEKIESEVNNRKIEL
                     GLKGGQAVLTLLTKKKITGLSSSLTKNRMLQESQAKLQEAQQVLASYQEELANLQEQL
                     TEQKTALIQKWDPTLLNVDEIRLTPTKQYIRIEKFGILWE"
     gene            complement(6275..6880)
                     /gene="recR"
                     /locus_tag="PLL88_07025"
     CDS             complement(6275..6880)
                     /gene="recR"
                     /locus_tag="PLL88_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOJ01358.1"
                     /translation="MPLLPEPLQRLTQAFERLPGIGPKTASRLAFFILKAPEQVSTEL
                     AEALNGLRLSIGTCPVCFNITLANQLRCDICGDEHRDGSILCVVEDPMDVLAIERAAG
                     FQGKYHVLHGAISPIEGIGPDDLRIRELEERVKAGGIQEIIIATNPSMEGDATALYLK
                     KQLAAYPVQITRLARGLPVGGDLEYTDQTTILRALAGRQDM"
     gene            complement(6893..7210)
                     /locus_tag="PLL88_07030"
     CDS             complement(6893..7210)
                     /locus_tag="PLL88_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOJ01359.1"
                     /translation="MAKGPKGMFNSENLLGQMRQMQEQLKIAQKELEKEEFSADIRGG
                     AIKVTFTGGQVCKAVEIDSDLCKNIDSQTMGVLLAEVINKALDKVRKQTMKKLGPISG
                     MGR"
     gene            complement(7213..8889)
                     /gene="dnaX"
                     /locus_tag="PLL88_07035"
     CDS             complement(7213..8889)
                     /gene="dnaX"
                     /locus_tag="PLL88_07035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOJ01360.1"
                     /translation="MTQAYYRKWRPAVWDEVIGQEAIVHTLRNAVAQEKQVHAYLFSG
                     PRGTGKTSTARILAKAVNCTHPDKSARPCEECENCKAVNAGRFLDLIEIDAASNTSVD
                     DVRDLREKINFAPSQGTFKVYIIDEVHMLSTAAFNALLKTLEEPPAHAMFILATTEIH
                     KIPATVLSRCQRYEFRQIPLNHITAQLKTIAEAEKLDYEMDALTMIARHATGSMRDAI
                     SLMDQIASSGDKITLALTEEVLGATSNENVIRLIESLIHEEINQAVSVIHTALDSGTD
                     GRQFSNQIVDYLRALVLMKIGNSKDVEATQELKRVMQAHSDEVSLEKLVTWVRIFNQA
                     QHNLGSRWQPSLALELAVVDCLEGPASLQQAVVERTPGNAPAMKTAPKPTARPTHEKK
                     GEEQVKPAPVPEKGREEQPITAAEKRAEQKTQETAPQSEPVDQAASQSGGSAENAVRD
                     HWKEIRALVRQEKPHTEALLNSCKSIRVEGKKLKLGFESDLLKTKMDTDINREITFKA
                     IHKLLGFDLQVECEVVKNKNNNHSTQSSEQHGHLVNAAISLGGEIIDKEQ"
     gene            9216..9288
                     /locus_tag="PLL88_07040"
     tRNA            9216..9288
                     /locus_tag="PLL88_07040"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9250..9252,aa:Glu,seq:ctc)
     gene            9610..10605
                     /locus_tag="PLL88_07045"
     CDS             9610..10605
                     /locus_tag="PLL88_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ01361.1"
                     /translation="MLDLKITMRRIARIVTFFLTLGLLCFPSAAVFSAGGATAADLIN
                     LIQTWRAEAGNPALIVDPLLNVTAYDTAYTMAAQGLHTHIGNASGRIAAYGYGDGSTV
                     RCTENFAMSYGEADISKIYGFWDDPDHRLPATYSYYKHVGAGVATTGNGWYYYVLHAC
                     YTSAGTYTPSGSGTSITTGDGTVVTATSAVSQIVVPVTTATPQPDGSVIHIVQSGQSL
                     WSIATAYGTKIDTIKQLNGLTSDSIYNNQELVVYPAGSMPTPLPTATLTPTSAPTLTP
                     TMTPTAMESTPQATPTPSRSRNAIPARTMAGYGIVLISVVGLLAMLVKLSIKKIK"
     gene            10728..12149
                     /locus_tag="PLL88_07050"
     CDS             10728..12149
                     /locus_tag="PLL88_07050"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOJ01362.1"
                     /translation="MPEQTISKTDFLLFLDCPLHFWALKHHYEPVILPTPMDEFRMQQ
                     GRAVEALALEHLRQWMKGQYPNAALLWQQTYLHGFFLARTDALIHDENEECFDLYEIK
                     SSTNMNKKDLFDVAFQAAVCQHTIPLRNLYLVHLNKNYRRQGSLDLNTLFLIEDITAA
                     VADLLPQTNAYMQAARTVLLQEEHANVEHCRKPDTCPFPDLCHPNIPEHSIYEISRLH
                     RSKALQLEAMGIRAIPEIPDDFPLSDQQREYVEVVKSGQPRVDPDAIQEELTNLHYPL
                     HFLDYETCNPAVPLFEGYKPNQHIVFQFSLHILSAPDAEAEHHQFLHAELSNPAPKLL
                     EKLSHTIQPQGSILVWNKGFECSRHNELAQLHPAYGKFLENINSRIFDLMDTFSHNFY
                     IHPAFHGSASIKKVLPVLVPDLSYEGMEISQGDEASTAWWQMVNGTFSEEEKENTIAH
                     LMKYCQLDTKALLEIFIRLQSIS"
     gene            12217..13224
                     /locus_tag="PLL88_07055"
     CDS             12217..13224
                     /locus_tag="PLL88_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ01363.1"
                     /translation="MDSFIPNLFSKPPTYRNLVFKGGGIRGIAYIGALEVLEERGILP
                     SIERVAGTSAGAISAFLVSFRIPVSEILKCFATLDFRKVPQASTHHRKLPIPLPVNPL
                     TEEEKLARIVRNYGLYSSSYFYQWLRSVVAEHCNGNPNATFEDFHERGFRDLYIVVAN
                     LNKHQGEIMSYHDTPQASVADAVRMSMSIPLYFEALRFDGKNFGKGDFYVDGGVYNNY
                     PIKLFDQPPFIKSTDLKRSKGNSETLGMFLYPEGGLKIIKKEDPRNLMEYLGVVISNL
                     YTAFETIQYENSFIDQHRTIAIPDRGILSTDFSIVKGDEKYNKLREAGRTAARAFLEN
                     L"
     gene            complement(13275..13664)
                     /locus_tag="PLL88_07060"
     CDS             complement(13275..13664)
                     /locus_tag="PLL88_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01364.1"
                     /translation="MTKNNLEDKITIIEGPPPVFEPIEDGWALGLTEGPLLFDTSLTQ
                     LRTFNGPELVERCHRVWKENGIMQLHYRNEMGVEEKVPIMAARSIETDEGQVLLLWTR
                     HTPEDFEEFDDLDDLGNDEDDEDDLEY"
     gene            13809..14231
                     /locus_tag="PLL88_07065"
     CDS             13809..14231
                     /locus_tag="PLL88_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ01365.1"
                     /translation="MSYFVYTPFGRAAHMRRMREFFDEDMPFSEREVMVPIDVRAEND
                     DFIVTALVPGLKPEDLNIQVINETVTLSGELKLYADEKANYIHKECPYGKFHRTITLP
                     STLDALKAEANVENGVLTLRVPKAEEAKPKTIKVSIAK"
     gene            14305..15096
                     /locus_tag="PLL88_07070"
     CDS             14305..15096
                     /locus_tag="PLL88_07070"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOJ01366.1"
                     /translation="MNKDELAALLHDLPLGELRWYESIPSTNACALEWLREFPKEYSL
                     VATNHQTAGYGRNQRPWHSTASASLTFSFILYPNQSEQGAVPLFSALAALSICDAVAS
                     FSPSAKIAIKWPNDVLLDGKKGAGILAEAAWQGRSLMGLVVGIGVNVRPAFLSSAAQE
                     LLFPATSLEECIGKLVERWELLHRIMAAFIARRDNFQAHAFMNAWREKLAFLGEEVTL
                     SRTGKELQHGTFLDVNEQGYLLLKEENGRISTFPLGDVSLRPRLS"
BASE COUNT         3817 a         4050 c         3445 g         3785 t
ORIGIN      
        1 agcatgccaa acggttggtt gaattctccc gcaaccaatc catagatagg ttctccttga
       61 tagggcatgg tctctttaga aaaatggatc gccccaccat aaactaccaa ctcgttccgc
      121 gtcatattct tagaaacaat ggggcacgcc atcgccaccg caatctcttc ttgggagcaa
      181 gcgcctagct gccattgctc aagatcgaaa aatataaaat tccccgggcg gatctcatcc
      241 actcctgaga aatcgtctat caatcggcat ccgggcgtat cgccaatgga taccagcaca
      301 tcctcaaaac cagccgccag cagaccatcc ttgactgcaa gcatagcctg ttggctggtt
      361 acagcaagtt ccttcacatt ctggggggat ttcgcatgat aagcttgccc ggcatgtgta
      421 agaattccac gaaaattcaa cctggcagag cgcatgatga ccctcgtcag cgtttcgata
      481 tgcgtttgat cttgccacca gatgccgctg cggtgcagac ctgtatcaat cttgatccac
      541 acatcgactg catgcctgac atgaaaagca agatattcaa cactttcctg acaatctacc
      601 aaaaccccca atcgaactct ttctgccaac gcgttcaaag cgtccatctc acgccaattc
      661 aaaggaaagg cgatcaagat atcttgccag cctgcgtcag cgaaatagcg cgccattgcc
      721 acagatgaca ccgtgatcgc actaacccct ctctttctaa acaattccgc tatacgactt
      781 gattggtgtg ttttgaagtg aggacggaaa gccactttat tcctgaccgc cttagcactc
      841 atggaggcaa tatttgcatc tgccttttgc agatcgacca gcaacgccgg cctctgaatc
      901 aggtccagaa agagcaattt caccaatttc cttttttcgc tataatcatg gtatggataa
      961 tgataccatc gctcgtatct tccttcgcat cgcgcgattg ctggagatta aaggagagag
     1021 caatttcaag atcactgcct atgagcgggc agcggatagc atccgcaatg ctcctgaaga
     1081 tgtatcagca atggatgaac aaaccctgca aaccatcccg ggcattggcg aagcgatcgc
     1141 aaaaaagatc acggaattca accaaaccgg agaactcaat tttctgaaaa aattagagac
     1201 cgaaattccc ccctctctct tagaatggct tgaacttccg ggagttggtc caaaaacaac
     1261 gactttgatc tggaaaacat tgggcattac aacgctcccc gagttgctag cggcagccaa
     1321 aagcgggaaa ttgcacgcct taccaggcat cggggaaaag ctggaaacaa agattttaca
     1381 agctatcgaa gaacaataat tgccatggtt aagaggggtt atggaagttt tccgacgtac
     1441 agttggttgc caaaagccag aaataattct tttcccatag acacccccac cccgctcatc
     1501 tccgtttcag gcaaaggata cgttttgctc ggttgcggtt ttagcgttcg ttctaaattt
     1561 aattggtaac tgtactggag caccgcccgg tttgccgaat ctaaaatgta aaaagcggat
     1621 gtgggataag tggtctcctg aatcgcgttg aactgtgcat caaaatacac caatggattc
     1681 ttttcatagc caatacggct atctccatac gctgtgggat cctggcattc tgtcgcgttc
     1741 ccttctcgat aggggttata ttgacacgtt gtcatgtggc tatcttgatg caaaatgaac
     1801 acatcctctt tgttgactat cagatccatt gccccaccca gatccacttc ttctgcccct
     1861 agaaaatcta cggggtgact gttgaaaaca atgcctgcca tagcagcaat ttcgaaatcg
     1921 ctccccgagt aactccaaat gccatctttt tctacatcca tcacatacag tgtgtattca
     1981 tccaagctta tcgcaccgat cttgccccat tcactcgccg gtgggatgag cgaactgggt
     2041 acagggtcac ttcctgataa gcaatacagc acattccccc ccgcatcaac cgcgagtatt
     2101 tcgaaggaac gcgtattggt tggcaacgtt gtaaaatcta caatctttcc caatgtgatc
     2161 actccgtagg tacctgggct gcaccgaaag gttgaatcga tctcataaaa accgcgggaa
     2221 ttggcaatcg cccgatacac actgccggcg gtggcatcca acagataaat acccgattca
     2281 ttgcttttga tcttggtaag caccacattt tgtgaaaatt gcgaggttgt ggcaggtctg
     2341 aaatccaaac gggtaaccag gtccatgtca tcgatgacgg tctgagtctg aacgagtaac
     2401 gtattcgcaa ggtcgctatc gccgaattcc atggccttta acaccgtttc gtatgctctc
     2461 gtccaatatt ctcgttgctg tgcggcatct tcgatggtgg tcgcctgggt gatataggat
     2521 tgcgcttcat ccaagaagac ctggtgttgt tcccggcgac cgctgtacac ataaaccagc
     2581 acagccatgg ttattaaaac gagaggaaca ataattacag ccgtactcaa caaacgggtt
     2641 ggagcgttac tttccaatgg ggtggaatca gcaataaacc agttgcttat tctgaggaac
     2701 agggtctgca atgcattgga tgatcgcttt ttttcggttc cttgtttgga cggtatccgg
     2761 cttttgacat actcactttt cttcccctta cgcgtctcac cttccaatgg agtctttctc
     2821 ttctcaaagt ctattctttc cggtcgccct ggttctacat tgagcgtttc ttcttccatt
     2881 tctggaaaga attgtcccag ggtcagatca tgatcggcct tggatgtgcg atgctgtacg
     2941 gtggggctta cctgctcttc aaccttggca gggatgtagt cttgttctgt cttttcttgt
     3001 actgcggctt gttcttcgat ttgctccaca ttccccgctt cggatgaaag agcaggggtt
     3061 tcaactgacc attgctcttc ttgtaccaag ccctttcctt ccttacattt aattaccata
     3121 gcattaagat caatgttcac ctggtccaat aacctgcgat aaacctgctc catgcttaat
     3181 tgctggctgt ttttgagatg gttttcatcc cacgttaggg gagcccgcgt tgagagcatt
     3241 atcaggtcac cggaggtcag gggcaactgt tgaaaattca agagaaagcg acgggcgcta
     3301 cccaacccac gttcaccgtc atcctggttc tgcaggtgaa caaaagagtc agcgccaatg
     3361 agaaaaatat gcgaagcccc ggtttgagct gtaaacagat atccattgtg agaaacaata
     3421 aaacatccag tcgctcgggc aggttcaacg gattccggtg caagattccg ttcaaataag
     3481 gcggtgttaa cggacgccac cgcagtttgc aaggcattcg tcaccgaacc tggggtttca
     3541 aaaaattttt ttcccgcgcc ggcgataatc tctttgatct cctcttcagc gatcaaagca
     3601 ggctcactga aatcgagctg caaagccaga acttctccct tacggtggcg atggctttta
     3661 aatgaactcg ccacaacaag agatcctttg gcagtagcac tcacgttgcc atctgctatt
     3721 ctcaatggat aggtatgaaa gttaatgccc atgttgtgct ctcatgtatg gtctcattat
     3781 attcacacca caaagtttgt aaaatatcta ctcattgggt gtttatccgt atacaataag
     3841 gatatcaaat aatggaatgg tgaggaatgg ttaatagaaa aaatagagag ctctatctgg
     3901 gaaagctttt cgatctttct gataaaaaat tgctaactga tcaaccatta atgtacgatc
     3961 tggatgatct cacaacccat gcaatcgtaa ccggtatgac cggatcggga aaaaccggtc
     4021 tatgcatcgg cttgctggaa gaagcagcca tgcagcaaat tccagccatc atcatcgatc
     4081 caaaaggtga tctgacgaac ctctttctgc actttccgga gttggcaccg gctgatttcg
     4141 agccctggat caatcctgat cttgccagag gggaaggaaa aaccatcccc gaaatggctg
     4201 ccatcactgc tgaaaattgg caaaaaggat tgaaggagtg ggaaattggg aaagaagaca
     4261 tcctgaaact tcaaagcagc gtagaacgcg ctatttatac tcccggctct tcccgaggta
     4321 tcccggtcaa tgtcatgaca tctttcgaag cgcccatgat ggactgggaa gaaaatgagg
     4381 atgtcttgcg cgaaatgatc tcaagcaccg tctctgcgtt gttgacattg gtcggtttca
     4441 aagacattga tccgctgcgg tcccgtgagc atattctgat cgccaacatc attgaggaca
     4501 gttggaaaaa gaacaactct ctcgacctga ccgaactcat tctgctcatc caggagccgc
     4561 ccattaccaa gctgggcgct ttccccatgg ataaattctt ccccaaaaaa gagcgggatg
     4621 agctggcgtt acgattgaac aacttcctgg cttctccatc cttccaatcc tggatcgaag
     4681 gacaatcgct ggatgtggca caactcctct tcgcgccttc cggcaagccg cgccacagtg
     4741 tcttttacct ggctcatctc aatgatacgg agcgcatgtt cttcgtcacc ctgcttttcg
     4801 cagccatcga cgcatggatg cgcaggcaaa gcggcactac cggcatccgc gtcttactat
     4861 actttgacga aattctcggt taccttcccc cggttgccaa ccctccttcc aaaccactga
     4921 ttatccgatt gctgaaacag gcacgcgcgt ttgggctcgg tttgatcctc accacccaga
     4981 atcccattga tgtggactac aaagcgcttt ccaacgcggg cacctggatc atcggacgcc
     5041 tgcagaccga ccaggataaa caacgtttgc tggacgggtt ggaaagcgcg gcaggcggtt
     5101 tgagccgatc tgtactggac aaaacaattt cttcgcttgg caagcgggtt ttcatgcttc
     5161 agaacgtcaa tgaaaaacaa cccatcattt tccagacgcg ttgggcaatc gattacctga
     5221 ccggtccgct taccatccag caaatccccg ccttgaacag gctggcaggc gcccaaatgc
     5281 cagagccggc gataggtgga gtggaaaaaa taagatcaga aacgtattcc acaacccctt
     5341 ccgccagttt atcctccact tctgctgcaa ttccatatat ccgcaaaggc agcggcgcca
     5401 gcggaaagaa tgcatcgctg ctggctcagg cagaagtcaa tttctattcg cgcacacccg
     5461 ccatcaacgc cagtcatgtg atcaccgtcg tcatccccaa tccaccccag cgcgcattgg
     5521 actgggagaa aagccgcatc gagaatctaa ccaaagatag cttcatccag gcgacagacc
     5581 tcaaaacagg gcaaaaggat gtgagcgaat ggttgctcga ttcaaaatgg tggatggaaa
     5641 ggcaaaaaga attcgaacag tgggtctatg aaagcgatag cctttctgtg tttcgcaaca
     5701 aagacaccgg attacaatcg caggcaggag aagatcggga atccttcgtc cagcgcctga
     5761 ccacagccac tcaggagaaa ttgaagaccg agcaagcagt gctggaacgc agctttcaat
     5821 ctaagctcct tagtgcgcaa aacaaggttg aacgccaaca attgcgtgtt gaaaaaatcg
     5881 aaagcgaagt caacaaccgc aagatagaat tgggactgaa aggcggtcag gcagtgctca
     5941 ctttgttaac caaaaagaag atcaccggtt tatccagttc cctcacaaaa aatcgtatgc
     6001 tccaagaatc acaggctaaa ctgcaagaag cccagcaagt tcttgccagt taccaagaag
     6061 aactggctaa tctgcaagag caattaacgg agcaaaagac agcgctcatc cagaaatggg
     6121 atcccacatt attgaacgtg gatgagatac gcctcacccc caccaagcaa tatatccgta
     6181 tcgaaaaatt tggcattctt tgggagtaga gaaagctgcg caatcaaaaa aggaatcagg
     6241 cacctgattt cttttttgat tttaacaatg cgggctacat atcttgcctg cctgccagag
     6301 cgcgcaggat cgtggtttga tcggtatatt ccagatctcc acctacgggc aaaccgcgcg
     6361 ccaggcgggt aatctggacc ggataagctg ccagttgttt tttaagatac aaagccgttg
     6421 catccccttc catgctgggg ttggtggcaa tgatgatctc ctgaatgcct ccggctttca
     6481 cgcgctcttc cagttcacga atgcgcagat catccggacc gatcccttca atgggggata
     6541 tagctccatg gagaacatga tacttacctt gaaaaccggc ggcacgttca atcgccaaca
     6601 catccatggg gtcctcgacc acacagagta tgcttccatc gcggtgttca tccccacaga
     6661 tatcgcagcg aagttgattg gcaagggtaa tattgaaaca gaccgggcac gtcccgatgg
     6721 ataagcgcaa tccgttcaaa gcctccgcca gctctgtgga aacctgttcg ggcgctttca
     6781 agatgaaaaa cgccaaacga gaagctgtct tgggtccgat tccaggaagt cgttcaaaag
     6841 cctgcgtcag gcgctgaagt ggttctggga gcagtggcat agacgagtac ccctaccgcc
     6901 ccattccact aataggacct aattttttca tggtctgttt gcggacctta tcgagcgctt
     6961 tattgatcac ttctgctaat aaaactccca tggtttgaga atctatgttc ttacaaagat
     7021 cgctatcaat ctccactgcc ttgcatactt gtccaccggt gaaagttacc ttgattgccc
     7081 ctccacggat atccgcactg aactcttcct tttccaactc cttttgcgcg atctttaact
     7141 gctcctgcat ctggcgcatt tgaccaagta aattctctga attgaacata ccctttggac
     7201 ctttagccat tgtcattgct ccttatcaat aatttcacca cccagagaga tggcagcgtt
     7261 cacgagatgc ccatgctgtt ctgacgactg tgtggaatga ttgttgtttt tattcttcac
     7321 cacttcgcat tcaacctgta ggtcaaaccc taaaagttta tggattgctt taaaggtaat
     7381 ttcccgattg atatccgtat ccatcttggt tttcagcaga tcgctttcga atcccaattt
     7441 cagcttcttc ccctcaacac ggatggattt acaggaattg agcagcgctt ccgtgtgcgg
     7501 tttttcctgc cgaaccaaag cgcggatttc tttccagtga tcgcgcacgg cattctccgc
     7561 tgatccgccg gactggcttg cagcttggtc aactggttcg ctttgtggcg ctgtttcttg
     7621 cgttttttgt tctgcgcgct tttctgctgc ggttatgggt tgctcttctc tgcccttttc
     7681 cggaacaggc gctggtttaa cctgttcttc accctttttt tcatgggtcg gacgggcagt
     7741 cggttttggg gcagttttca tcgctggggc attgccaggg gtcctctcca ccaccgcttg
     7801 ttgaagcgat gccggtccct ccaggcaatc caccactgcc agttccagcg ccaggctggg
     7861 ctgccagcgc gaaccgagat tgtgctgcgc ctggttaaag atgcggaccc aggtcaccaa
     7921 cttctctaaa ctcacttcat cgctgtgcgc ttgcatcacc cgtttcaatt cctgggtagc
     7981 ttcaacatct ttgctgttgc cgatcttcat cagcaccaat gcgcgcaggt aatcaacgat
     8041 ttgattcgaa aactgcctcc catccgtccc actatccaaa gccgtatgga ttacggaaac
     8101 tgcctgattg atttcttcat ggattaagga ctcaatcagc cggatcacgt tttcattgga
     8161 ggtcgctcca agcacttctt cggttaaagc cagggtaatt ttatccccac tggaggcgat
     8221 ctggtccatt aatgagatcg catcgcgcat actacctgtg gcatggcgcg cgatcatggt
     8281 gagcgcatcc atctcataat ccagtttctc cgcttcagca atggttttga gctgggcagt
     8341 tatgtggtta agcggtatct gtctgaactc ataacgctgg caacgtgaaa gcaccgtagc
     8401 cgggatctta tggatctcag tggtagcgag aatgaacatg gcatgagcag gaggttcttc
     8461 gagagttttc aacaaagcgt tgaatgcagc cgtagagagc atgtgcactt catcgatgat
     8521 gtacaccttg aaagtgccct gcgaaggggc gaaattgatc ttttctcgca agtctcgcac
     8581 atcatccacg ctggtgttgg aagccgcatc gatctcgatc agatcaagaa aacgaccggc
     8641 gttgactgct ttacagtttt cgcattcttc acaagggcga gcggatttat cggggtgcgt
     8701 acaattgacc gctttcgcca gaatgcgggc tgtcgaagtc ttgcctgttc cgcgtggtcc
     8761 ggagaacaaa taggcgtgta cctgcttttc ttgtgctaca gcgttgcgca aagtatgaac
     8821 aatagcttcc tgcccaatca cttcatccca aaccgcaggg cgccattttc gatagtatgc
     8881 ctgtgtcatg tctatccttt atgtagaaat tttccttaca ggaattatag ccacaaatat
     8941 ctctcttgag cgttaaaaca tccgaatcct gcgttctttt ctaacatttc gttaattatt
     9001 aatcggataa tttggatata ctatatccgc ggataccaag acctttacga gtagtggtat
     9061 cgccaccgat gggtagcgta tccctcctta tgctacgtga ctccgcgagg agtatcggtg
     9121 gcaaatcctc ctttggactg ccggtgaaac tccggcagtt tctttttagc gcatttgaca
     9181 accaaaatcc cacatgctac aattcaaaat tgcctgcccc cattgtctag cggtctagga
     9241 cgtggccctc tcaaggccaa agcaggggtt cgagtcccct tgggggcatt tttattttcc
     9301 aaagtttctt gctcttcccc acgtccgtga atgttttctt tattcacaaa ctgttcagcc
     9361 aacgcggatg gtataaagaa cgcgcggctg ctttggtgtg ccggtcattt ccttcagcct
     9421 cattcgtttt atggctgtaa gcatgtgagg tggtgatatc gtccccaaag gggaacgcga
     9481 caaaaccgta aaattttcac ttgcaaaacg agatccctct aaacagtgat ttttcaaagt
     9541 gtcctctcat gcgtatgaac aaataccttt tcctctttcc tatatatccc attggtttgt
     9601 agaaaaatca tgttagactt gaaaattact atgagaagaa tagccagaat tgtgaccttc
     9661 tttttgaccc tggggcttct ttgctttcca tctgctgcgg tcttctctgc cggcggcgcc
     9721 acggctgccg atctcatcaa cctcatccag acctggcgcg cagaggcagg caacccagcc
     9781 ctgatcgtgg acccactctt gaacgtcacc gcctacgaca ctgcctacac catggctgcg
     9841 cagggcttgc acacccatat cggtaacgcc tcgggcagaa tcgccgctta cggctacggg
     9901 gatggcagca cggtacgctg caccgaaaat tttgccatga gctatggcga agcggacatc
     9961 agcaagatct atggattctg ggatgatccc gaccatcgcc tgcccgccac atactcctac
    10021 tacaaacacg tcggcgcagg ggtggctacc accgggaatg gctggtacta ctatgttctg
    10081 cacgcctgtt acaccagcgc cggcacctac actccttccg gttcaggaac cagcatcacc
    10141 accggcgatg ggacggtcgt caccgccacc agcgcagtct cacagattgt tgtgcccgtt
    10201 accacagcaa cgccccagcc cgatggcagt gtgatccaca ttgtccagag cggacaatct
    10261 ctgtggtcca tcgccaccgc ttatggcacc aagatcgaca ccatcaaaca actgaacggt
    10321 cttacttcag attcaattta caacaaccag gaactggtgg tgtatccggc aggcagcatg
    10381 cccaccccgc tgcccacagc cactctgacc cccaccagcg ctccaacgct cacccccacc
    10441 atgacaccca cggcgatgga gtctaccccg caagccactc ccaccccatc ccgcagcaga
    10501 aacgccatcc ctgcccggac tatggcggga tatggcatcg tgctgatctc tgtggtaggg
    10561 ttgctagcca tgctcgtcaa gctttcgatc aagaagataa aataaaaccc atggtttccc
    10621 tcgagggaag attccataac aaaagctttg gaatcttccc gtagatttaa gcttcgccaa
    10681 cttgagatgg cgttcctcta ctataatcat tgcagagggt aaatatcatg cctgaacaga
    10741 ccatcagcaa gaccgatttc ctcctttttc tggattgtcc gctgcacttc tgggcgctca
    10801 aacaccatta tgagcccgtc atcctcccca cccccatgga tgaattccgc atgcagcagg
    10861 ggcgggcagt ggaagcgctc gccctggagc atctccggca atggatgaaa ggtcagtatc
    10921 ccaacgcagc tcttctatgg caacagacct atctgcacgg cttcttcctg gcgcgcaccg
    10981 atgcgcttat tcatgatgaa aacgaggaat gctttgacct ttacgagatc aaaagcagca
    11041 caaacatgaa taagaaagat cttttcgacg tcgcctttca ggcagcggtt tgccagcaca
    11101 ccatcccgct gcgaaatctg tacctggtgc atctgaacaa aaattatcgc agacagggca
    11161 gcctcgatct gaacaccctg tttctgatcg aagatatcac cgccgcagtt gcagacctgc
    11221 ttccacagac caacgcctat atgcaagccg cgcgcacggt tctattgcag gaagagcacg
    11281 cgaacgtcga acactgccgc aaaccggaca catgtccctt ccctgacctg tgccatccca
    11341 atatacccga acattccata tacgaaatca gtcgcctgca ccgcagcaaa gcgctccagt
    11401 tggaagcaat gggcatccga gcgatcccag agatacccga tgacttcccg ctctcggacc
    11461 aacaaagaga atacgtggag gttgtaaaaa gcggtcagcc ccgcgtcgac ccggacgcca
    11521 ttcaagaaga attgaccaac ctgcactatc cgttgcactt tctggattat gaaacctgca
    11581 accctgccgt tcccctcttt gaggggtaca agcccaacca gcacatcgtt ttccaatttt
    11641 ccctccatat cttgtctgct ccggacgcag aagcggaaca ccaccagttc ctgcacgctg
    11701 aattgagcaa cccggctccc aagctgctcg aaaagttatc gcacaccatc cagccgcagg
    11761 gcagcattct ggtgtggaac aagggttttg aatgttcgcg gcataatgag ctggcacaac
    11821 tgcaccccgc atatggaaag tttctggaaa acatcaacag ccgcatcttt gacctgatgg
    11881 acaccttcag ccataacttc tacattcacc ctgcttttca tggtagcgca tccatcaaga
    11941 aagtgctgcc cgtgctggta ccggacctct cgtatgaggg gatggaaatt tctcaaggcg
    12001 atgaagcctc cactgcctgg tggcaaatgg tgaatggcac attcagtgag gaggagaagg
    12061 aaaacacaat cgcccatctc atgaaatatt gccagctcga tacgaaagct ttgttggaaa
    12121 tcttcattcg cttgcaatca atttcttgat tgccattttt tacatgtttt tttgtttcgt
    12181 gtatcattca gacattgatt attccaagag gtgtatatgg attcatttat acccaatctg
    12241 ttttccaaac ctcccaccta ccgcaacctg gtcttcaaag gcggcgggat tcgcggcatc
    12301 gcctatattg gggcgctgga agtgctggaa gaacgcggca ttctgcccag catcgaaagg
    12361 gtagccggca cttctgcggg cgccatttcc gcttttctgg tcagttttcg catcccggtc
    12421 agcgagatcc tgaaatgttt cgccaccctc gatttccgca aggtaccgca ggcttccacc
    12481 caccatagaa aacttcccat accgctgccg gtaaatccgc ttaccgaaga agaaaagctc
    12541 gcccgcatcg tcagaaatta cggactttac tccagttcct acttctatca atggttgcgc
    12601 tcggtggtag ccgagcactg caacggcaac ccaaatgcca ccttcgaaga tttccacgaa
    12661 cgaggttttc gcgatctgta catcgtggtc gccaatctaa acaagcacca gggagagatc
    12721 atgtcttacc atgacacgcc gcaggcatca gtggcagatg ccgtgcgcat gtccatgtcc
    12781 atcccgctct atttcgaagc tctgcgcttc gacggaaaga attttggaaa gggcgacttt
    12841 tatgtggatg gcggggtgta taacaattac cccatcaaat tgttcgacca acctcccttc
    12901 attaaatcca ccgatctcaa acgcagcaaa ggcaattcgg aaacgctggg gatgttcctt
    12961 taccctgaag gtggcttgaa gatcataaaa aaggaggatc cacgcaacct gatggaatat
    13021 ctgggcgtcg tgatcagcaa tctgtacacg gctttcgaga ccatccaata tgagaacagc
    13081 ttcatcgacc agcaccgcac catcgctatc cctgaccgcg gcatcctatc tactgatttc
    13141 tcaattgtga aaggcgacga aaaatacaac aagttgcggg aagccggtcg caccgctgcc
    13201 cgcgcttttc tggaaaacct gtaaaataca aaaaagcgaa ccgcgtgggt tcgctttttt
    13261 gagcttacga gatcctagta ctcaagatcg tcttcatcat cctcgtcatt gcccaggtca
    13321 tccagatcat caaattcctc aaaatcttct ggcgtgtggc gagtccaaag caataacacc
    13381 tgaccctcat cagtttcaat agaacgggca gccatgatgg gtaccttttc ttctacaccc
    13441 atttcattgc gataatgcag ttgcataatg ccgttctctt tccatacacg gtgacagcgc
    13501 tctaccagtt cgggaccgtt aaaagttcgc aattgggtaa ggctcgtatc aaacaataaa
    13561 ggtccttctg taagaccgag cgcccagcca tcttcgatcg gctcaaatac cggcggagga
    13621 ccttcgatga tggtgatctt gtcttccaga ttgttctttg tcattcttat tctccgaagc
    13681 cacaatcata ccacttttca aaccacgtca tccataattt taataaaaga attagatttt
    13741 gcttattctt tttttatctt tgccttttat gattacaacg taagcattaa gaaaatggag
    13801 gtttgaacat gagctacttt gtttatacac ctttcggcag ggctgcccac atgcgcagga
    13861 tgcgcgagtt ctttgatgag gatatgccct tctcagagcg ggaagtgatg gtgcccatcg
    13921 acgtgcgtgc tgaaaatgac gatttcatcg tcaccgcgct ggtgccagga ttaaaacccg
    13981 aagatctgaa cattcaggtg atcaatgaga cagtcacttt aagcggtgaa ttgaagctgt
    14041 acgccgatga gaaagccaat tacatccaca aggaatgccc ttacggaaag ttccatcgca
    14101 ccatcacttt acccagcacg ctggatgcac tgaaggcaga agccaatgtg gaaaatggtg
    14161 tgctaaccct gcgcgttcca aaagccgaag aagccaaacc aaagactatc aaagtcagca
    14221 ttgccaaata aaataaggta gtaaccaact cccaaaaaga cagccaatga gctgtctttt
    14281 ttggttccag actataatgg aaaagtgaat aaggatgaac ttgctgctct gttgcatgac
    14341 ctgccgctgg gagagttgcg ctggtatgaa tctatccctt ctaccaatgc ctgcgccctg
    14401 gaatggctgc gcgaatttcc caaagaatac tccctggtag ccaccaatca ccaaactgcc
    14461 ggttacgggc gcaaccagcg cccctggcat tctacagcca gcgcttccct taccttcagt
    14521 ttcattctgt acccgaacca aagcgagcag ggcgctgtgc cgctgttttc agcactggct
    14581 gccctttcga tttgcgacgc ggttgcctcg ttttctcctt ctgccaaaat tgccatcaaa
    14641 tggccgaatg acgtcttgtt ggatgggaag aaaggagcag gcatcctggc tgaagcagcc
    14701 tggcagggac gctctttgat ggggctggtt gtgggaatcg gcgtcaatgt tcgaccggca
    14761 ttcctatcat ccgctgcgca ggagctgctc tttccggcaa ccagcctgga agaatgcata
    14821 ggcaaactgg tggaacgctg ggaattgctg caccgcatca tggcggcatt catagccagg
    14881 cgcgataact ttcaggcgca tgcgttcatg aatgcctggc gcgagaagct ggcttttctg
    14941 ggcgaagagg tcaccctatc cagaacagga aaagaattgc agcatggcac ctttctggat
    15001 gtgaatgagc agggatatct gttactgaaa gaagaaaacg ggcgcatctc gacatttccg
    15061 ttaggggatg tgagcctgcg cccgcgtttg agctgat
//
LOCUS       DAOSDS010000050        15003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1586_length_15003_cov_4.966417, whole genome shotgun sequence.
ACCESSION   DAOSDS010000050 DAOSDS010000000
VERSION     DAOSDS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15003
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1586_length_15003_cov_4.966417"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..321
                     /locus_tag="PLL88_07075"
                     /pseudo
     CDS             133..321
                     /locus_tag="PLL88_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
     gene            347..1303
                     /locus_tag="PLL88_07080"
     CDS             347..1303
                     /locus_tag="PLL88_07080"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOJ01367.1"
                     /translation="MPKREEINRLVMIAREYYVNRKTQAEIAEEFDLSRPTISKLLQE
                     AEDANIVQISIIDPLYRDSELASRLQDALQLQNVVVVHGDPKNQGISQKNIALNAAWY
                     FCKMVDDTVHKIGIGWGRTMYQVSLAMETAVRNDLQFIPLLGGVGQINPSFQVHQITQ
                     RVADVCGGTWLQLHVPGIIENKEIRKNLLNSRDVQNVISNWENLDLALVGIGEAPPFK
                     RNIIFESYVDDEEKTQLLEKKAVGDICMRFFDIHGKIINYLKQEIISIPLNVLKNTQR
                     VIAAAGGSRKARSIIGASRGGFITDLVTDEDTGNEILQILNQ"
     gene            complement(1308..1928)
                     /gene="cyaB"
                     /locus_tag="PLL88_07085"
     CDS             complement(1308..1928)
                     /gene="cyaB"
                     /locus_tag="PLL88_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HOJ01368.1"
                     /translation="MHTNDDHEREVKFFIADLKEMFSRLSAAGAVKRKERVHEYNLRF
                     DTPHQSLRSQHQVLRLRQDDQTHLTFKGQTDLSQGIADRRELEITVSDFDSARKILEG
                     LGFVVFLIYEKFRTTYQLNACEIVLDELPFGYFMEIEGETAQVIQTVAQKLNLDWEKR
                     ITLSYLELFNNLKTNKNLHMENIVFESFQNRKFIESDFFIEPIASP"
     gene            2054..3052
                     /locus_tag="PLL88_07090"
     CDS             2054..3052
                     /locus_tag="PLL88_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ01369.1"
                     /translation="MTSIYDIAKAAGTSIATVSYVINKSGRVSPETAARVEKAIHDLQ
                     YEPKSFARALASGQTFTISVVSPLTAYKNQISFSKLLNGVGNVLQKSDYRLFIHPTLG
                     RADSWMELEAAVRGHQMDGVILMHVQMQDKRVAVLKKESMPFVLIGRTEDNTGLDYVD
                     ADISGAVRLAVEHLIERGHRKLCMVGEKGNAGISIRLIEQFKIMISEKDLPTQPSWCS
                     NMAESSDEMIHDLAEILSAKNRPSAIFAVSDLAVLNTLKVARSLHLRIPEDIAVIGYA
                     ESSIYKHLDPPISAVFNGVEKLGSLAAQILLNKLTDPEKPIEQVLVPPELVQRHSV"
     gene            3127..4398
                     /locus_tag="PLL88_07095"
     CDS             3127..4398
                     /locus_tag="PLL88_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOJ01370.1"
                     /translation="MFTKSQWLRLAIILTLIMGLIAGCTNKEEVKATEEAQVEQKEPV
                     TLNVWILDRVGTDAMAAVSAVIQAWAAETGNTVNITEGNQFEMLNNIPVAIPAGEGPD
                     VFMTINNYVGGHYLGGLIVPIQDSFADADSYIPGALDAFTVDGQLLGVPVAADVNALV
                     YNKAKVAEAPATMQELISVSKELTEGDEYGLLYQIDSFWYSYPFFSAHGGYIFKWTGS
                     GWDTSDLGINTSEGVAGLQYIRDMVTVENLMPADTTWEVMNAYFTEGKSAMIITSPTM
                     IPSFKEAGIEVGVAPLPTIEGVGTPKPFATYTGFSVSAYSEHQEEAINLVQYIGANSG
                     KPLYEANSGNIPVYSSVLNDPALSSDVELAAWMSQLANSDPLPNIDAMNYVWTPVTTA
                     FQTVVHGEADAATALAEAQELILQSLQGVTQ"
     gene            4462..5394
                     /locus_tag="PLL88_07100"
     CDS             4462..5394
                     /locus_tag="PLL88_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ01371.1"
                     /translation="MPAKIQKNQKPIYPYFYLLPGLMAILFSIVIPGVMTIYYSFTNY
                     SLRHLQDWGWIGWENFEKIFVGSNQGEFLGVFSWNMQWALISTIGSALVGLILAMLLN
                     NKRVVERNLYRVLLILPWALPSTITILTWKGLFNSSFGPINRMLGSIGMAAIPWLSNS
                     TWARVTCLLVNIWIGYPFMMSAFLGGLQSIPEELYDACQVDGANGWQTFRHITLPMLR
                     SIVLPLLVSGFAMNFGNFGVIYLLTEGGPFKSPSVLAGATDLLSTYMYKVAFGATNFD
                     YGLASANGLLIFLIVGSLTLINLSMTGAFREVKE"
     gene            5391..6248
                     /locus_tag="PLL88_07105"
     CDS             5391..6248
                     /locus_tag="PLL88_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ01372.1"
                     /translation="MSHSPLSRSKKKTRIFLSTNLTRLLLIISCIVVLFPIVWMATAS
                     ISKGSSLFSSTIIPKNITVKNYVNLFEKTKFIKWIRNSAIVTLCGSLLALLETTTMAY
                     AFSRFKFKGRKNGLMILILLQMLPATVTIVALYKLAVFIQLQNTLLGLILIYAGTTIP
                     FNAWLMRGYFDSIPFEIEESAYLDGANHWQAFTKIAFPLCLPMTVVVFLFNMIGFYND
                     YYLASIILSGNNMYTVALGMRFFTQPYAQNWPMFAAASILACLPIVIIFYSLQRYLVQ
                     GLTKGALKG"
     gene            6251..7357
                     /locus_tag="PLL88_07110"
     CDS             6251..7357
                     /locus_tag="PLL88_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ01373.1"
                     /translation="MQNSHSFTGLEKPCPDVIRFIDHLLGKVQLPTPPLMEFLVDDSI
                     MRRVNTEVLGRTWVVPGMDRESQIAYLDNVIAFWKELGYDYVRMEMALPFPARANSTQ
                     NTAADNPGMRTWVDEKKGAITNWQDFEAYPFPDVSKFDFFALEYLNLHVPEGMGLILS
                     HGGGIFERTSWIFSMEGLCYSLADDPALVQAVSERVGNLQLQFYRQILDLDRVAAIFP
                     GDDMGFKTATLISPRDLRRYFLPWHKKYAGLAHEKSLPYFLHSCGNILKIMDDLIEDV
                     KIDGKHSYEDAIIPVQQFQQMYKGKIAILGGMDMHFLSTASVGQVKVRTRNLIELCGQ
                     AGRYAVGSGNSIADYIPMENYLAMVREVNLLKNG"
     gene            7370..8437
                     /locus_tag="PLL88_07115"
     CDS             7370..8437
                     /locus_tag="PLL88_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ01374.1"
                     /translation="MIFHDILHYKPDLQHVIRRYRDLYEHPEKKQMLVYAMLPFENKI
                     PVPALNSLNLQKNIPSYLDICLKNYEHSLEGSRTIEDDLIPTFGINFGTGEYSAFVAG
                     EVVFTQDTSWATSVIQEWNDLKKLKVDEGNRWVVFMEETLRYVLQFTQEGPIPVVRGF
                     YSPLDLAHALRGEMIFTDLYDAPKEVEALLDFCADAIIWLAKRIHGMIGTYWDGSVAG
                     GWLKPGTICMSEDIACLISPKTYARQIRPYTQKVINAFGNGQIHTHSLGLRTLPEITK
                     LDHLLGVQIAEDPNTPRTMSNLEKVLETANDVPLTITCTFEEMKEKIDVVAGRYNIVF
                     GSSANNVEDCEAIVRWIRERG"
     gene            8440..10212
                     /locus_tag="PLL88_07120"
     CDS             8440..10212
                     /locus_tag="PLL88_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ01375.1"
                     /translation="MLDFIFGDMVSEEERIEKYRRTVRGVSHLSRRNPLQARPGKEVR
                     LALSIGPAVVTQEAFVYYTTDGSDPIGSKGRATRGFALAMQKDIVEWNMPEWGYVQIY
                     TAVIPAQKSGTVVRYRLSSLAPAGEEIFADDGKYYAFSVDEYQTPQWAKEAILYHIMV
                     DRFSPGSGKSWNSIQDVEDIYGGTLRGIIEHLDYIQELGANTLYLSPLFPCNSHHGYD
                     STDYFEIEPRFGSKEDFKELLDALHQRGMKFIMDFIPNHWSNRHATFQDAIANPNSPY
                     RDWYVFRNYPDDYECFFEVKEMPKINLRNTDARRHILNAACYWLKFGVDGFRIDYAIG
                     PAPDFWADFRSAAKRTKADCWIFGEVVDPPDSQKALVGLMDGCLDFHLLEFIRKCLGS
                     REWDFEKFSAFLEKHFSFFPDDFLLPSMLDNHDMDRFSWIAGKDGRLLKLAALTQCFL
                     PNPPVLYYGTEVGLSQNTGIRSEYGGFGHLGEARLPMIWNEEQDRDLYRFFKTLYQLR
                     KDLAFHERREISFEKVMPTSLLLSFKSDSPIWLLLNIGEKEEQFEIESRTAPIAFCTA
                     HCQVSEDHGKYQLTLPAFSGAILY"
     gene            10222..11304
                     /locus_tag="PLL88_07125"
     CDS             10222..11304
                     /locus_tag="PLL88_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOJ01376.1"
                     /translation="MTRILQQITNPRINENGVTWFYRGKQSPRLIGDFNFWNAEKALL
                     FAKNQDGSWETHLELPEDAYLEYALWINGKRKLDPLNKLKVHNGLGEWNNYFYMPRFV
                     ETPYLQERSGKSLFRTESHILRDPLRLTNGKRCVHFYIPTDKGSFPLLVIFDGQEYLE
                     NGKVIQIIEALIDQGKIQPIAVALVDNIYQNRFVEYACNDGTVSFITRNVISAARSIM
                     PLLDTGEQKGAFGIMGASMGGLMALYSALREPGIFGKVLCQAGAFKMYEDDFSIYEFV
                     DGVITRPLKIWMNVGCFDFLCEENLRMKDLLLKKGYSTDFVMNNGGHNYTTWRNSLHL
                     GLMKMFPPKKKIKKDSPCQKIFYSKQ"
     gene            11274..11906
                     /locus_tag="PLL88_07130"
     CDS             11274..11906
                     /locus_tag="PLL88_07130"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOJ01377.1"
                     /translation="MSENILFKAVVDGERDRVIKAIREAIERGEKADGILQEGIVKGM
                     AEVGRLFEEGEYFVPEMLIAARAVKDGLQVLKPYLQLADIQPRGKVLIGTVKGDLHDI
                     GKNLVSIMLEGAGYQIIDLGVDVDVALFLEKTKEEKPDILALSALLTTTMVNMAEVVK
                     TIRNEKQIQNVKIIIGGAPVTQNFADEVGADGYSIDASQAVRLVDHLMQH"
     gene            11989..12318
                     /locus_tag="PLL88_07135"
     CDS             11989..12318
                     /locus_tag="PLL88_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HOJ01378.1"
                     /translation="MKNKDDLISRDVFEHLVDLAAFALAEDEKNYLHEQMNHQLAAIR
                     ALEAIPLPGDLVATTHGVEYGKDGKPTLRNDEWKAYENTKEILAQAPDLQDGYIVVPD
                     IPTTELQ"
     gene            12318..13916
                     /locus_tag="PLL88_07140"
     CDS             12318..13916
                     /locus_tag="PLL88_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HOJ01379.1"
                     /translation="MADLTELSALELTALVRSRQVSAAEILDANLVRIHEVDGRPGAL
                     DGSLTTEDEKKVHAFITHTNEAALQQARAVDARIAQGEDAGLLAGVPFTVKDIFCVRG
                     TPSTAASRILANFTAPYTATPVARLTAAGGLMLGKVNLDEFTYGSSSESSAFQPSPRN
                     PWDLSKVPGGSSGGSAAAVAAGEGALSLGTDTAGSIRQPATFCGVVGLKPTYGRVSRY
                     GLIAFGSSLDCPGPIARNVKDAALMLQVIAGGDSADSTASTRAVPDYLQDIEKGVKGM
                     RIGLSPDYFRITFPDPESGDYIEQPLPQEMQEAVQKAAEWYRVMGAEVIEDVPMPNTR
                     FGIPVYFVISRVEAASNLHRFDGVKYGYRTQKAVKDLNEMYRRSRGEGFGLQPKLRIL
                     MGMYVSAAQYSEQYYQRALKVRTLIRRDFENIFDPNGKYQLDALLTPTTPTPAFGMAA
                     VYGDSVLMQYADQLTVPANHAGIPGISIPAGFAHDGLPIGIQLLGADFSEAKLLRIAR
                     AYETATEKEAWRSRKPAVLEGKRA"
     gene            13913..>15003
                     /gene="gatB"
                     /locus_tag="PLL88_07145"
     CDS             13913..>15003
                     /gene="gatB"
                     /locus_tag="PLL88_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOJ01380.1"
                     /translation="MSIEYEAVIGLETHIQLNTHTKIFCACKADSWDDAPNSNICPVC
                     TGLPGVLPVLNEKVVEKAVLLAAAMQAGIQPLSFFDRKNYFYPDLPKGYQISQYDQSL
                     ARGGYLDLPIPQTADPEGKGYVRRVRIHKLHIEEDAGKTKKERNRRYVDFNRCGVPLV
                     EMVTEPDLRSAEEAGQYLIRLRQLLRWLGISEGDMEKGNLRCDANVSIRKKGSSALNT
                     KTEIKNVNSIDAVKDAIRKEIERQIREVKKGNAIKPWTLDWDDNAGVLRMMRSKESEA
                     DYRYFHEPDLLPLEISNSWKEEILKDVPELPLERRTRFMRDFQLPEYDADILTTDREL
                     ADYFEESVRFYQGDVKKISNWLMNDVLRMR"
BASE COUNT         3947 a         3319 c         3860 g         3877 t
ORIGIN      
        1 acatatgggc tcataaagct cctgctgcca tgacgcgtgc cattgcagcg gtggtgcacg
       61 aaggttcatc ggtcggcgaa gccgtgaaat acctgagctg agccaaaata ccgaaaagga
      121 gtcgatgata gacttccgcg aaagcttttc caaccgttgg tgggtggtac aaccgatggg
      181 gtaaaattaa agatggacga ttttgagcac gcattggatg agtattatgc gcacatgggg
      241 ttgcagaaag aaaccggaag accaacgctg gagatgttga ggacgctcga tctggaagag
      301 ctgagtgcag gattagaata aaaaaagcca gtatgtggag aagagaatgc ctaagcgcga
      361 agagatcaat cgactggtaa tgatcgccag ggaatattat gtgaacagga aaactcaagc
      421 ggagattgct gaagagtttg atctatcacg tcctacaatc tctaaattgc ttcaggaagc
      481 ggaggatgcg aacatcgttc agatcagcat catcgatcct ttatatcgcg attcggaact
      541 ggcgagtcgt ttgcaggatg cactgcaatt gcaaaatgtt gttgtggtac atggcgaccc
      601 caagaaccag ggtatatcac aaaagaatat cgccctcaat gccgcctggt atttctgcaa
      661 aatggtcgat gataccgtac ataaaattgg cattgggtgg ggcaggacga tgtatcaggt
      721 atcccttgcc atggaaacag ccgtgcgcaa tgaccttcaa ttcattccgc ttttgggtgg
      781 ggtgggtcag atcaacccga gttttcaagt tcatcagatc acgcagcgcg tcgctgatgt
      841 ttgtggagga acctggttgc aattgcatgt cccgggcatc attgaaaaca aggaaatcag
      901 aaagaatctc ctcaattccc gcgatgtgca aaatgtgatc tccaattggg aaaacctgga
      961 tctggctctg gtgggtattg gcgaagcgcc accgttcaaa cgcaatatca tttttgaaag
     1021 ttatgtggat gatgaagaaa agacccaact gctggaaaag aaagcagtgg gtgatatttg
     1081 tatgcgcttt tttgatatcc atggaaagat catcaattat ttgaaacagg agatcatcag
     1141 catacccttg aacgtcttga agaacacgca gcgggtgatc gcagcagcgg gcgggtcacg
     1201 caaagcgcgt tccatcatcg gcgccagccg gggaggattt atcaccgatc tggtcaccga
     1261 tgaagatacc ggaaatgaaa tcttgcaaat attaaatcaa tagcacgcta tggtgaggcg
     1321 attggttcaa taaagaagtc cgattcgatg aattttctgt tttgaaagct ttcgaaaaca
     1381 atgttctcca tgtggagatt tttgtttgtt ttcaggttgt tgaataattc caaataactc
     1441 aaggtgatgc gtttttccca gtccagattt aatttttggg ctacggtttg gatgacctgt
     1501 gcggtttccc cttcgatttc cataaagtac ccgaagggca gctcgtcaag gacaatttca
     1561 cacgcattca actggtaagt ggtgcggaat ttctcataaa taagaaaaac aacgaacccc
     1621 aagccctcca gaatttttct ggcagaatca aaatcactga cggttatttc caattcccgg
     1681 cgatcagcga tcccctgcga taaatctgtt tgacccttga acgtaaggtg cgtctgatca
     1741 tcctggcgca agcgcagcac ctgatgctgg gagcgcaagc tttgatgggg ggtgtcaaaa
     1801 cgcagattgt attcgtgcac gcgctctttt cgcttaactg ctccagcggc tgacaggcgt
     1861 gaaaacatct ccttcagatc ggcaatgaaa aatttcactt ctcgttcgtg gtcatcattg
     1921 gtatgcatgg tttttattat cctataattt cgagaactga ggaagaggga cttgaatttc
     1981 atgcactgat acgctataat ttaagcgctt aagcaattat ttttttacct tgttgcttaa
     2041 gcgcttaaac cttatgacct cgatttatga tattgctaaa gctgccggaa cgtcgatcgc
     2101 aaccgtttct tatgtgatca ataagagcgg gcgggtaagc cctgaaactg cagcgcgtgt
     2161 tgaaaaagcc attcatgacc tgcagtatga accgaaaagc tttgcacggg cactcgccag
     2221 cgggcaaacg tttaccatct ccgtcgtttc cccattgacc gcatataaaa accaaatatc
     2281 tttctccaaa ttgctcaatg gcgtaggcaa tgttcttcag aaaagcgatt accgtttgtt
     2341 catccatccc acattgggca gggctgattc atggatggaa ttggaagcgg cggtgcgcgg
     2401 tcaccagatg gatggcgtga ttctcatgca tgttcaaatg caggataagc gtgttgctgt
     2461 tctgaaaaaa gaatccatgc cttttgtttt gatcggacgc acggaagata ataccggcct
     2521 ggattatgtg gatgcagata tcagcggtgc tgttcgcctg gcggttgaac atttgataga
     2581 acgaggtcat cgaaagttat gcatggtggg agaaaaagga aacgcgggaa tttccatccg
     2641 cttaatcgag cagttcaaaa taatgatctc agagaaagac cttcccactc aaccatcctg
     2701 gtgttcaaac atggctgaat catccgatga aatgatccat gaccttgccg aaattctctc
     2761 cgcaaaaaat cgcccttctg ctattttcgc agtttccgat ctggctgtgt tgaatacctt
     2821 aaaagtcgcc cggtctttgc atttgcgcat accggaagac attgccgtca ttgggtatgc
     2881 tgagagttcg atctataaac accttgaccc ccctatcagc gccgtgttca atggggtgga
     2941 aaaactgggc agtctggcgg ctcaaatatt attaaataaa ttgacggacc ctgaaaaacc
     3001 aatagagcag gtccttgttc cccccgaact tgtccaaaga cattcggtat agaagaaaag
     3061 gaggtggaag agatcagatt cttttatctt ctgttaaaaa aataaacaat aatcttggag
     3121 gattgaatgt ttacaaagtc acaatggctg cgtctggcta tcattttgac cctgattatg
     3181 ggtttgatcg caggatgcac caacaaagaa gaagtcaagg caacagaaga agcgcaggtt
     3241 gaacaaaagg aaccggtcac cctgaatgtt tggatcttgg atcgcgtggg gacagatgcc
     3301 atggcagctg tcagcgctgt cattcaagca tgggctgccg agaccgggaa tactgtcaat
     3361 atcaccgagg gaaaccaatt tgaaatgctc aataatattc ctgttgccat ccccgcaggt
     3421 gaaggaccag acgtgttcat gaccattaat aattacgttg gcggtcacta tttgggtgga
     3481 ctgattgtcc ccattcagga ttcatttgcc gatgctgatt cctatattcc tggtgcgctc
     3541 gatgctttta ccgtggatgg tcaattattg ggtgttccgg tggcagcgga tgtcaatgcc
     3601 ctggtataca acaaagccaa agtagcggaa gctccagcaa caatgcagga attgatcagt
     3661 gtttccaaag aactcaccga gggtgatgag tatggattgc tttatcaaat tgatagtttc
     3721 tggtatagct atccattctt ctccgcacac ggtggatata tcttcaagtg gaccggcagc
     3781 ggttgggaca cttcagacct tggcatcaat acctctgaag gtgttgccgg acttcaatac
     3841 attcgtgaca tggtaactgt ggaaaatttg atgccagcag atacaacctg ggaagtgatg
     3901 aacgcctatt tcactgaagg caaatctgcc atgatcatca ctagcccgac catgatcccc
     3961 tcattcaaag aagcgggcat tgaggttggc gttgcacctc ttcccaccat tgaaggggtt
     4021 ggcactccca aaccatttgc aacctatacc ggtttttctg ttagcgcata ctccgaacat
     4081 caagaagaag cgatcaatct cgttcaatat atcggcgcga atagcggaaa acccttgtat
     4141 gaggcaaatt ctggcaacat cccggtctat tccagcgtgt tgaacgaccc cgcgctttca
     4201 tcggatgtag aattggctgc ctggatgagc caattggcga acagtgatcc attacccaat
     4261 atcgacgcca tgaactatgt ttggacgccc gtcacaaccg cattccagac cgttgttcat
     4321 ggtgaagcgg atgccgctac ggcattggca gaagcgcaag aattgatcct tcagtctctt
     4381 cagggagtta cacaatagtt tttaaggaaa atagagttgg gatttttccc aactctattt
     4441 taatttggag cgtccatggg aatgccagca aagatccaaa aaaaccagaa accgatctat
     4501 ccatattttt acctgttgcc cggacttatg gcgatcctct tttccatcgt cattccgggt
     4561 gtgatgacta tttactattc attcaccaat tattctctgc gccatcttca agattgggga
     4621 tggatcggat gggaaaactt tgaaaagata tttgtgggaa gtaatcaggg agagtttttg
     4681 ggagtctttt catggaacat gcaatgggcg ttaatctcca ccatcggttc tgcactggtt
     4741 ggtctgattt tagccatgct gctcaataac aaaagggtgg ttgaaagaaa cctttaccga
     4801 gtgctgttaa tattaccctg ggcgctaccc tcgacgatca cgattttaac ctggaagggg
     4861 cttttcaact cttcttttgg accgatcaac cgcatgttgg gcagtattgg gatggcagca
     4921 ataccttggt tatccaattc aacctgggca agggtaacct gtttactggt caatatttgg
     4981 atcgggtatc cattcatgat gtctgctttc ttgggtggat tgcagagcat ccccgaggaa
     5041 ctttatgatg cctgccaggt agatggggca aatggatggc aaaccttcag gcatatcaca
     5101 ttacccatgc tgaggtcgat cgttttaccg ctgctggttt ctggctttgc catgaacttt
     5161 ggcaattttg gtgtcattta tttgctgacc gaaggcggtc ctttcaaaag tccatcggtg
     5221 ttggcggggg cgactgacct tttatcaacg tatatgtata aggtggcgtt tggagcaacc
     5281 aattttgact atgggctggc ttctgccaat ggtttgctga tcttcttgat cgtgggcagt
     5341 ttgacgttga tcaacctatc tatgacaggt gcatttcgtg aggtgaaaga atgagccatt
     5401 cccccttatc ccgttcaaaa aagaaaaccc gcatttttct ttctaccaat ttgacacgtc
     5461 tcctattgat cattagctgc atcgtagtac tttttccaat cgtgtggatg gcgaccgcgt
     5521 ccatttcaaa gggttcttca ctattctctt caaccatcat tcctaagaac attacagtga
     5581 agaactatgt caatctgttc gaaaagacga aattcatcaa atggatacgc aacagtgcga
     5641 tcgtcacttt gtgtggcagt ctgttggcgc tgttggagac caccaccatg gcgtacgcct
     5701 tttcacgatt taagttcaaa ggcaggaaga atggattgat gatcttgatc ttgctgcaaa
     5761 tgttgcccgc cacagtcacg atcgtggccc tgtataaact ggcagtgttc atccaattac
     5821 aaaacacatt gctggggttg atcttgattt atgcaggaac caccatccct ttcaatgcct
     5881 ggctgatgcg cggatatttc gattccatcc cgttcgagat tgaagaatcg gcatacctgg
     5941 atggagcaaa tcactggcag gcttttacca agatcgcttt tccgctgtgt ttgccgatga
     6001 ccgtggttgt ttttcttttc aatatgatcg gtttttataa cgattattat cttgccagca
     6061 tcatcttgag cgggaataac atgtatacgg tggctctggg gatgcgtttc tttacacaac
     6121 cctatgctca gaattggccc atgttcgcag ccgcttctat ccttgcctgc ctgcccattg
     6181 tcattatctt ttactcgctt cagcgatatc tggtacaagg tttgaccaaa ggcgcactca
     6241 aaggataagt atgcaaaaca gtcattcgtt taccggactg gagaaacctt gtccggatgt
     6301 aatacgattc atcgaccatc tcttagggaa agtgcagctc cccacgcccc ctttgatgga
     6361 atttctggta gatgacagta tcatgcgcag ggtcaacacg gaggtacttg gacggacctg
     6421 ggtggtgccg ggtatggacc gggaatcgca aattgcttat ctggataacg tgatcgcatt
     6481 ttggaaagag ctcggttacg attatgtgcg catggagatg gctcttcctt tccccgcaag
     6541 agcaaacagt acgcaaaata cagcggcgga taatccggga atgcgcacgt gggtggatga
     6601 aaaaaaaggt gccattacga attggcagga ttttgaagca tatccatttc cggatgtctc
     6661 aaaattcgat ttctttgcct tggaatattt gaaccttcat gtgccagaag gaatgggatt
     6721 gatcctatca catggaggag gaatctttga gcgaacctcc tggatatttt ccatggaagg
     6781 gctttgttat tctctggcag atgaccctgc tctggtgcag gcggtctctg aacgggtggg
     6841 aaatctgcaa ttgcagttct accgccagat ccttgatctt gatagggttg cggcgatttt
     6901 tccgggtgat gacatggggt tcaagactgc cacattgatc tcgccacggg acttgcgccg
     6961 gtatttttta ccctggcata aaaaatatgc cggtctggca catgagaaga gcctcccata
     7021 tttcttacat agttgcggca atatcttgaa gatcatggat gatctgatcg aggatgtaaa
     7081 aatcgacgga aaacattcct atgaagatgc cattatccct gtgcaacaat ttcagcagat
     7141 gtacaaagga aagattgcca ttctgggtgg catggatatg cattttcttt ccaccgcatc
     7201 cgtggggcag gtgaaagtac gcacaagaaa tctcatcgag ctttgcggac aggccggtcg
     7261 ctatgcggtc gggtcaggaa attccattgc cgattacata ccgatggaaa attatctggc
     7321 gatggtgcga gaagtaaatc ttttaaaaaa cggataaagg aaaagaatca tgatctttca
     7381 cgatattctg cattacaaac ctgatcttca gcatgtaatc cggcgctacc gagacctgta
     7441 tgagcaccct gagaagaaac aaatgctcgt ttacgccatg ctgccttttg aaaataagat
     7501 cccggtgcct gctttgaact cattgaacct gcaaaaaaat atcccatctt atctggacat
     7561 ttgcttgaaa aattatgaac attcccttga gggaagccgt acgattgaag atgaccttat
     7621 cccaactttt gggatcaatt ttggaacagg ggagtacagc gcttttgtgg ctggagaggt
     7681 cgtcttcacg caagatacat catgggcaac ttccgttatt caagaatgga atgacctgaa
     7741 aaaactgaaa gttgacgaag gcaatcggtg ggtcgttttt atggaagaga ccttgcgcta
     7801 tgtattgcaa ttcacccaag aaggtccaat tccggtggtg cgcggtttct attctccact
     7861 tgaccttgcc catgctttgc gcggagagat gattttcacc gatctgtatg acgctccgaa
     7921 ggaagtggaa gcgttgttgg atttctgcgc tgatgccatc atctggttgg caaaaaggat
     7981 ccatggaatg atcggaactt attgggatgg cagtgtggct ggaggatggt tgaaaccggg
     8041 cacgatctgt atgtcggaag atatcgcttg tttgatctct cccaaaacgt atgcaaggca
     8101 aattcgccct tacacacaaa aagtgatcaa tgcctttggc aatggtcaga tccatactca
     8161 ttctctgggg ttgcgcactt tgcctgagat cacaaaactg gaccacctgc tgggtgtgca
     8221 gatcgcggaa gatccgaaca cgccgcgtac gatgagcaat ctggaaaaag tgttggaaac
     8281 cgccaacgat gttcctttga caatcacctg taccttcgag gaaatgaagg aaaaaattga
     8341 tgtggttgct ggaagataca acattgtttt tggctcctca gcgaacaatg ttgaagactg
     8401 cgaagcgatt gtgcgttgga tacgggagag aggataagca tgcttgattt tatttttggc
     8461 gacatggttt cagaagaaga gcgcattgaa aaataccgca ggacggtgcg cggtgtatcc
     8521 catctttccc gccgcaatcc tcttcaagcc cgtccgggaa aggaagtacg ccttgctctc
     8581 tccattggtc ctgccgttgt aacacaagaa gcttttgtgt attacaccac cgatggatcg
     8641 gaccccattg gatccaaagg gagagcaacc cggggctttg cgcttgcaat gcaaaaggat
     8701 attgtggaat ggaacatgcc tgaatggggg tatgtgcaga tctataccgc ggtcatccct
     8761 gcacagaaat cgggaaccgt tgtgcgctac cgcttgagct cgctcgcccc tgccggcgaa
     8821 gagatctttg cggatgacgg aaaatactat gcgttcagcg tggatgagta ccagacccca
     8881 caatgggcga aagaagccat cctgtaccac atcatggttg accgcttctc tccgggttct
     8941 ggaaagtcct ggaattccat ccaggatgtt gaagacattt acggcggcac gctgcgcgga
     9001 atcattgagc atttggacta catccaggaa ttgggagcga acacactgta tctcagcccg
     9061 ctctttccct gtaattccca tcacggctat gattccaccg attattttga gatcgagcca
     9121 cgttttggat caaaagaaga ttttaaagag ctgctcgatg cacttcatca gcggggcatg
     9181 aaatttatca tggattttat tcccaaccac tggtccaatc gccatgctac ttttcaagat
     9241 gccattgcca atccgaacag cccctatcgc gattggtacg ttttccgcaa ttaccccgat
     9301 gattatgagt gtttctttga agtgaaagaa atgccgaaga tcaatcttcg caatacggat
     9361 gcacgtcggc atattctgaa tgcggcttgc tactggctga aatttggcgt ggacggtttt
     9421 cgtattgatt atgccattgg acccgcccct gatttttggg ctgacttccg ctctgctgca
     9481 aagagaacga aagcggattg ttggatcttc ggagaggttg ttgacccacc cgactcacaa
     9541 aaagcattgg tggggttgat ggacggttgt cttgactttc atcttctgga attcattcgc
     9601 aagtgtttgg gttccagaga atgggatttt gagaaatttt ccgcttttct tgaaaagcat
     9661 ttctctttct tcccagatga tttcttgctg ccatccatgc ttgataacca cgatatggat
     9721 cgtttttcat ggattgcagg gaaagatggg cggttgctga aattggctgc tctgacccag
     9781 tgttttctac caaatccacc ggtgttgtat tacggaacag aagtgggtct ttcgcagaac
     9841 actggaattc gcagcgaata tggtggtttt gggcatttgg gtgaagcgcg gctgccgatg
     9901 atctggaatg aggagcagga tcgagatctg tatcgatttt tcaaaacatt gtatcagtta
     9961 aggaaggatc ttgctttcca tgagcggcga gaaatttcct ttgaaaaagt aatgccaact
    10021 tccttgctcc tctcgttcaa aagcgattct cctatctggc ttcttctcaa catcggtgag
    10081 aaagaagaac agtttgaaat agaaagtcga acggctccca ttgctttctg cactgcacat
    10141 tgccaagtga gcgaagacca cggtaagtac caattgacct tgccggcttt ttcgggtgcg
    10201 attctgtatt aggaaatgcc gatgacaagg atacttcaac aaataacgaa tccacgaatc
    10261 aatgagaatg gggtcacctg gttttatcga ggaaagcaaa gtccgcgcct gattggcgat
    10321 ttcaattttt ggaatgctga aaaagcactg ctgttcgcaa aaaatcaaga tggatcgtgg
    10381 gaaacccatt tggaattgcc agaagatgcc tatctggaat atgctctttg gattaatggg
    10441 aaaagaaaac tggaccctct gaataaattg aaagtgcata atggtctggg tgaatggaat
    10501 aattattttt acatgccgag gtttgtggaa actccctacc tgcaagagcg gtcagggaaa
    10561 tctctttttc gaactgaatc gcacatcttg cgtgacccat tgaggttgac caatggaaaa
    10621 cgatgtgtcc acttctacat ccctaccgac aagggatcgt tcccactgct ggtcattttc
    10681 gatgggcagg aataccttga aaatggaaaa gtgatccaga tcatcgaggc actcattgac
    10741 caagggaaga ttcaacccat tgcggtggca ctggtggata atatttacca aaatcgattt
    10801 gttgaatatg cctgtaatga cggaacagta tcctttatta caagaaatgt gatttctgct
    10861 gctaggtcga tcatgccgtt gttggatacg ggtgaacaaa aaggtgcgtt tggaattatg
    10921 ggcgcatcca tgggaggatt gatggctctt tacagtgcat taagggagcc gggcattttt
    10981 ggaaaggttc tttgccaggc aggagctttc aaaatgtatg aggatgactt ttcaatctat
    11041 gaatttgtgg atggtgtgat taccagaccg ttgaagatct ggatgaatgt gggctgcttt
    11101 gattttttgt gtgaagagaa tttacgcatg aaagaccttt tgctgaaaaa gggatattca
    11161 acagactttg ttatgaacaa cggtgggcat aactacacaa cctggcgcaa ttctcttcac
    11221 ttggggttga tgaagatgtt tccaccgaaa aagaaaatta aaaaggattc accatgtcag
    11281 aaaatattct attcaaagca gtagtagatg gagaacgtga tcgggtgatc aaggcgatcc
    11341 gggaagccat tgagagaggg gaaaaagctg atggtattct gcaggaaggg attgtaaaag
    11401 gaatggcaga ggtaggtcgg ctttttgaag aaggggaata ctttgtgcct gaaatgttga
    11461 tcgctgcacg agccgtaaag gatggtttgc aggtgctgaa gccctatttg caattggcgg
    11521 atattcaacc tcgcgggaag gttttgatcg gaaccgtgaa aggtgacctg catgatattg
    11581 gcaagaatct ggtttctatt atgctggaag gagcgggata ccagatcatt gatctggggg
    11641 tggatgtgga tgttgccctg ttcctggaaa aaaccaaaga agaaaaaccg gatatcctgg
    11701 cactttctgc gctgttgacc accaccatgg taaacatggc tgaagtggta aaaacgatcc
    11761 gcaatgaaaa acaaatccaa aatgtgaaga tcatcattgg gggcgcgcca gtgacccaga
    11821 attttgcgga tgaggtcggg gcggatggct attcgataga cgccagccaa gcggttcgat
    11881 tggtagacca tttgatgcag cactgattcg catgaccgcc gacaatgtct tgacatgact
    11941 ttattcttga ttatactcac ctcagatcga agatacttga gagagataat gaagaacaaa
    12001 gatgacctga tctcgcgtga tgtgtttgaa catttggtcg acctggcagc ttttgcgctg
    12061 gcagaagatg aaaaaaatta tttgcatgaa caaatgaatc atcagcttgc ggcgatccga
    12121 gcactggagg cgatcccgct accgggcgat ctggtggcaa ccacgcatgg tgtggaatac
    12181 ggcaaagatg gaaagccaac cctgcgcaac gatgagtgga aagcgtacga aaatacgaag
    12241 gagatcctgg cacaggcgcc agacctgcaa gacggttata tcgttgttcc agatatcccg
    12301 accacggagt tgcaataatg gcggatctga ccgagttatc tgcgctggag ctgacagcgt
    12361 tagtccgatc gcgccaggtg tcggctgcgg aaatattgga tgccaatctg gtgcgcatcc
    12421 atgaagtgga tgggcgcccg ggtgcgttgg atggctccct tacaactgaa gatgaaaaaa
    12481 aggtgcacgc atttatcacc cataccaatg aagcggctct gcaacaggcg cgggcggtgg
    12541 atgccaggat cgcgcaagga gaagatgccg gtcttttggc aggtgtgcca ttcacggtga
    12601 aggatatctt ttgcgtgcgg ggaacgcctt ctacggctgc ctcgcgaatt ctggcgaatt
    12661 tcacggcacc ctacacagcc accccggtgg cgcgcctgac ggctgccgga gggctaatgc
    12721 tcggaaaggt gaacctggat gaattcacct acggttcttc cagcgaatct tccgcttttc
    12781 agccgagccc acgcaatccg tgggacctta gcaaagtgcc cggcggttcc tctggcggca
    12841 gtgcggctgc ggtagcggca ggggagggcg cgctttcctt aggaacagac acggcaggat
    12901 ccatccgcca accggcaact ttctgtgggg tggtgggatt gaagccaacg tatgggcgcg
    12961 tatcgcgtta tggattgatc gcttttggtt catcgcttga ttgccccgga ccgatcgcac
    13021 gcaatgtgaa ggatgcagca ctcatgctgc aggtcattgc cggaggagac agtgcagact
    13081 ccaccgcttc caccagagcg gtgcctgatt atttgcaaga tatagagaag ggtgtcaaag
    13141 gaatgcgtat tggcttgtcg cccgattatt tccgcatcac ctttcctgat ccagaaagcg
    13201 gtgactatat agaacaacct cttcctcaag agatgcagga agctgtgcag aaggcagcgg
    13261 aatggtatcg ggtcatgggg gcagaggtca tcgaggatgt gcccatgccc aacacgcgct
    13321 tcggcatccc ggtctatttt gtgatcagcc gggtagaggc ggcttccaat ttgcatcgct
    13381 ttgatggcgt caaatacggg tatcgcacgc aaaaagcggt gaaggatttg aacgaaatgt
    13441 atcgcagatc gcgcggcgaa gggttcggct tacaaccaaa actgcgcatc ttgatgggca
    13501 tgtatgtcag cgctgcccaa tactcggagc agtactacca acgagcattg aaagtgcgca
    13561 ctttgatcag acgcgacttc gaaaacattt ttgacccaaa tggaaaatat caacttgatg
    13621 cgctgctcac ccccaccacg cccaccccgg catttggaat ggcagcggta tatggtgatt
    13681 cggtgttgat gcaatatgcc gatcagctca ccgtgccggc gaatcacgcg gggattccgg
    13741 gaatctccat cccggcgggt tttgcgcacg atggcttgcc gatcggcatc caattgctag
    13801 gggcagattt cagcgaagcg aaactgctgc gcatcgcccg cgcttatgaa acggcgacgg
    13861 aaaaagaagc atggcgcagt agaaaacctg ctgtactgga ggggaagcgg gcatgagcat
    13921 cgaatacgaa gcggtgatcg ggctggaaac ccatatccag ttaaacacac acaccaagat
    13981 cttctgtgcg tgcaaagcgg attcctggga tgatgccccc aacagcaaca tctgcccggt
    14041 gtgcacgggt ttacccggag tgctgccagt gcttaacgaa aaggtggtgg agaaagcggt
    14101 gctgctggca gccgccatgc aagctgggat tcaacccttg tctttctttg accggaagaa
    14161 ttatttctat cctgatttgc ccaaaggata ccagatctct caatatgacc aatcgttagc
    14221 caggggcgga tatcttgacc tgcccatccc acaaactgcc gaccctgagg gcaaaggata
    14281 tgtgcgccgg gtgcgcatcc ataaactgca cattgaagag gatgccggaa agaccaaaaa
    14341 ggaaaggaac cgccggtatg tggacttcaa ccgctgtggc gttccgctgg tggaaatggt
    14401 taccgagccg gacctgcgtt cggcggaaga agccgggcaa tacctcatcc ggttgcgcca
    14461 gttgctgcgc tggctgggta tttcggaagg ggatatggaa aaaggaaatc tgcgctgcga
    14521 tgccaatgtc tcaattcgaa aaaagggcag cagcgcgttg aacaccaaga ccgagatcaa
    14581 gaacgtcaac tctatcgatg cggtcaagga tgccatccgc aaagagattg aacgccagat
    14641 cagagaggtg aaaaaaggaa acgccatcaa accctggacg ctggattggg atgataatgc
    14701 gggtgtgctg cgcatgatgc gctccaaaga aagcgaagcg gattaccgct attttcacga
    14761 acccgatctg ctgccgttgg agatcagcaa ctcctggaaa gaagaaattc taaaggatgt
    14821 gccggaattg ccgctggaac ggcgcacccg attcatgcga gattttcaac tgccggaata
    14881 tgatgccgat attctcacca ccgaccgcga actggcggat tatttcgaag aaagcgtcag
    14941 gttctatcaa ggtgatgtga agaagatttc caactggttg atgaacgatg tgctgcgcat
    15001 gcg
//
LOCUS       DAOSDS010000051        14858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1604_length_14858_cov_4.563872, whole genome shotgun sequence.
ACCESSION   DAOSDS010000051 DAOSDS010000000
VERSION     DAOSDS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14858
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1604_length_14858_cov_4.563872"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="PLL88_07150"
     CDS             <1..427
                     /locus_tag="PLL88_07150"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOJ01381.1"
                     /translation="HLGYSWSEAHEDAELLEHITPLRLTSRLDKFLNYPAYCPHGSAI
                     PHTDGQIDSVPLQTLNLLSAGETSHIRKVTEERELLDYLQNLEIEIGSAITVVSVGAY
                     EGPLTLALDGGKRVQISYKAACQIYVDQAAQHNTTITSQ"
     gene            439..1377
                     /locus_tag="PLL88_07155"
     CDS             439..1377
                     /locus_tag="PLL88_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOJ01382.1"
                     /translation="MNKKSQHFSGIALSCVLLLAACAQPAANPEPTEDTLNVVATTTM
                     LADLSRVIGGEYVTVSGLMGPGIDPHLYQASAGDVTRMQEADVVVYNGLHLEGKMGEI
                     FENLSGQGHAVICIEDGLDESKLLAWEGGGSVHDPHIWFDVAIWKQAAQIVAEGFANA
                     DPAHSDAYKGNLEQYLKELDELDTYVRDRVSEVAEGQRVLVTAHDAFNYFGKAYGFEV
                     RGLQGISTEAEAGTADVSALADFIVERQIKAIFVESSVPPKTIEALQAAVKAKGFDVS
                     IGGELYSDSLGGEGSGAESYILTVKANIDTIVEALK"
     gene            1374..2147
                     /locus_tag="PLL88_07160"
     CDS             1374..2147
                     /locus_tag="PLL88_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOJ01383.1"
                     /translation="MTQVVDMERSAQGYAVEVEDLTVAYDTKPVLWDIDLKIPKGKLM
                     AVVGPNGAGKTTLIKAMLGLLTPVSGCIRFINGSDNVRSLKNRIGYVPQSGSVDWDFP
                     ATVQDVVLMGRYGKLGWIKRPRKADYELTLQMLKKVGMDAYAYRQINQLSGGQQQRVF
                     LARALVQEADVYFMDEPFKGVDAQTEKAIVLLLKELKGQGKTVVVVHHDLQTVADYFD
                     WVTLINLRVIASGPVEEVFYEENLKKTYRSTGTLLRSAT"
     gene            2149..3072
                     /locus_tag="PLL88_07165"
     CDS             2149..3072
                     /locus_tag="PLL88_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOJ01384.1"
                     /translation="MNAWISLFNDYTFQTVALGSALLGLISGVLGSFAVLRKQSLLGD
                     GISHSALPGVVMAFILLGSKNTETLLLGALISGLTATLFIVGIVRHTRIKFDSALALV
                     MSVFFGLGLVLLTYVQKIPNSNQAGLSRFIFGQASTLLQRDIILMAVCGAVLLALVIL
                     FWKEFKLSTFDSDFAQSLDFSPKKLNLLLSFMIVLSIIIGLQTVGVILMSAMLIAPAV
                     AARQWTNKLWGMVLLSAIFGVVSGIVGTAISSLVAKLPTGPVIVVCISVIVMVSVLFA
                     PGRGILHRLYQRRKNRLLLKMEGGEPNVPTT"
     gene            3056..4174
                     /locus_tag="PLL88_07170"
     CDS             3056..4174
                     /locus_tag="PLL88_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOJ01385.1"
                     /translation="MSPQLEIQLIAVIVAVTCALPGAFLVLRKMSMMSDSITHTILLG
                     IVLAFFMTHDLSSPLLILGAALMGVVTVWLTELLGRTRLLAEDAAIGIVFPLLFSIAI
                     ILITRYAGSVHLDTDSVLLGELAFAPFDRMVVAGMDIGAKAIYTTGALFLINLAVIII
                     FFKELKVATFDPMLAAVLGFAPALVHYGLMTLVSLTAVSAFQAVGSILVVAFMIGPPV
                     TAYLLTDDLKWMLILSGLIGATNGILGYQAAALLDVSIAGSMAVMTGIVFLLVFIFAP
                     HRGLVSALRRRRNQKIQFAKMTLLFHLYNHEGSENEASEAGIDDIHTHLRWDEAFTKS
                     ILALLKKEDCIKFTDGVIKLTDQGRSNSLRNYEALFSE"
     gene            4541..5551
                     /locus_tag="PLL88_07175"
     CDS             4541..5551
                     /locus_tag="PLL88_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01386.1"
                     /translation="MSDWKTVLKADPTNWLLEEENPSVRYFTLKDILGRAEDDKAVCT
                     ARQDVMQTGMVPMILDKQREAAYLQSYSRFYTYKYKGLVWSLIVLTELGAEANAQIKE
                     HCEYLFENSQERQDGGFSQNTAARAGGGRITEVIPCLSGNMVWSLIRFGYLDDTRLQK
                     GIDWITRYMRFNDGVADDPQVPPYDRYEICWGAHTCHMGVVKALKALSAVPVERRTKE
                     INDTIQKAAEFLLIHHIYKRSHNLNRTSKPGWLKFGFPLMYQTDVLEILDILTELGIE
                     DSRMDEAINIVIAKQDDTGRWKTENTYNSDRLLIPMGQKDEQSKWITLRAMRVLKRYQ
                     GR"
     gene            5883..6725
                     /locus_tag="PLL88_07180"
     CDS             5883..6725
                     /locus_tag="PLL88_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01387.1"
                     /translation="MTVAQPYQSDVDAILAKRYDNGADYWTTPDRRLLKGGPFSTLEC
                     AFMLLELGMEPSDLLLKETADLFFSAWREDGRFKLSPQGAIYPCYTINATNVLCHLGY
                     AADSRLQRTIDHLLEIQHSDGGWRCNKFSFGRGPETEFSNPGPTLAALNAFRFTDLLN
                     KEPALDRAVEFLLDHWTTRLPLGPCHYGIGTLFMQVEYPFANYNLFVYVYVLSFYDSA
                     RRDKRFLDAFGALESKVVDGKIVVERVNRKLAGLTFCKKGEPSDLGTMRYHEILKNLS
                     CVKT"
     gene            7048..7728
                     /locus_tag="PLL88_07185"
     CDS             7048..7728
                     /locus_tag="PLL88_07185"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ01388.1"
                     /translation="MLKKFFLNTRSPEGMGGVIMLWLMNVGHSSMSQWGLKQLAVKPD
                     DNILDIGCGGGANIARMLDMTPHGKVCGVDYAALSVEKSIQRNRKAIAAKRAEIKQGD
                     VSQIPYADSTFDIVTAFETVYFWQDFPNDLKEVARVLKPGGTFFICNEAVRLEGEDPP
                     HKGFIKMLDLNMYSANDFRQTLLAAGFESVNVILAKHKNMICVMAKKPGARKTSPTED
                     DLFSETSL"
     gene            complement(7842..8246)
                     /locus_tag="PLL88_07190"
     CDS             complement(7842..8246)
                     /locus_tag="PLL88_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOJ01389.1"
                     /translation="MQNNWPAGVKRTRQRESVLSILENSEKPLSAPDIFSQVEEGGDD
                     AWLSTVYRILELFVRKGVVIKTNVMNNEMAVYELNRFKHKHYAVCMSCHKIIPMDNCP
                     MEKFVPKLEDEDFHVMGHNLEVFGYCKDCNPT"
     gene            complement(8494..9309)
                     /locus_tag="PLL88_07195"
     CDS             complement(8494..9309)
                     /locus_tag="PLL88_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOJ01390.1"
                     /translation="MLNYGFMQNAIFVSMFIAILCPCIGIFLVLRRYSMIGDTLSHAS
                     LAGITIGLMANQNPILGAFIFTSICGALIEFLRSYFKKYTDLILTIVLSLSVGIAITI
                     ISSGKLRANADSFMFGSILTVTRLDVVLVLILSAISVLTLILLYHQMIYIAYDEDAAK
                     VAGVKVGLINYVFFVLVASAISVSIRIVGVLVLSSMIALPVATALQLGKGFKLTLIFS
                     IVFSVIDIMLGLFVSYYLNVAPGGFTALVSVAVLVLVLIAKRLQSSISAARSK"
     gene            complement(9302..9979)
                     /locus_tag="PLL88_07200"
     CDS             complement(9302..9979)
                     /locus_tag="PLL88_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOJ01391.1"
                     /translation="MIKAEKVFFSYTGSPPYVLDGINLKIRTGDYMSVVGENGSGKST
                     LLRLILRFIKPTSGSILSQAKRVGYVPQKNDFSNANFPITVYEALNSYRKLLKIKNRG
                     VITESLERVGMTNFTGVLMGALSAGQNQRILIARALMGDPDLLILDEPLNGVDTNSQK
                     EIYDFLKKINRENGITIVSVEHNLDAVISNSTLIYHLMGGQGHLCTPRRYADEFLKYR
                     GKDDTHA"
     gene            10129..11043
                     /locus_tag="PLL88_07205"
     CDS             10129..11043
                     /locus_tag="PLL88_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOJ01392.1"
                     /translation="MLKKWTAFLLCLIVIVSFSACSSNDHPADETEESKLNVVVTFNA
                     MKEFVAAVGKDKVEILTIIPDGVEPHDFEPKAQDLVALSTAEVFVYSGLGMETWVEEA
                     INAANNANLIIVEAAKGADAIENTEPEEVEEHGQYDPHIWLSLKGAELEAKAIKDALV
                     QADPANKDYYEQNCADFVAQLEDLFNEYNEKFQSVEKKSFVTGHAAFAYLCRDFGLEQ
                     NSVEDVFAEGEPSARQLAELVEYCREHNVTTIFAEEMASPDVSQTLANEVGATVETIY
                     TIESNEDDMTYLERMRDNLSKIYESLTK"
     gene            11307..11870
                     /locus_tag="PLL88_07210"
     CDS             11307..11870
                     /locus_tag="PLL88_07210"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ01393.1"
                     /translation="MSLEHAILGFLNYQPYTGYDLKKIFDASVQHFWSADQSQIYRTL
                     GKLEEQELVKMEKVAQEDRPDRKVYHITPKGKEELLRWLAGPPLMGAPHSSPLVQVFF
                     GGLLPDEELLIKFEGFAQMMRAVLAQYEQVPESIETFKKEIPSRREHYFWMLTLDNGI
                     RNMRANLEWVENIIQQIKNGEVPQEQE"
     gene            11874..12638
                     /locus_tag="PLL88_07215"
     CDS             11874..12638
                     /locus_tag="PLL88_07215"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ01394.1"
                     /translation="MIKQIPLRQRVRKFILIAVFFSFPITMNYLSPYLILEGASQGIV
                     SGSLITFTFLFISSLFLGRAWCGWVCPGGTIQDIVEPVNNKLIHGKRIDWVKWLVWIP
                     WVSLIGWLFIRSGGIRQINPLFGTEGGISVAGSAERPIMFAYFIYYMVIVLFSGLALI
                     VGRRAGCHSICWMAPFMMIGRWLRNRIGWIALRLQATPSACVDCKICNGHCPMSLPVN
                     QMVLDNKMENAECILCGSCVDFCPKDAIRYTFSANR"
     gene            12652..13923
                     /locus_tag="PLL88_07220"
     CDS             12652..13923
                     /locus_tag="PLL88_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01395.1"
                     /translation="MKITILNSSQTSAALDGYINTLVNLLESKGHEVQNLLLREMNLR
                     SCRGCFHCWVKTPGYCVLKDDSDLLDRTVISSDFVLWASPMVLGFPNEFFKRALDKHL
                     PLLLPNMEIAEGEMHHVARYPHYPRIGMLVEKERATDEEDVRILTDIFARTALNFKTR
                     LEFLATTQEAPEQISEQILHPQEGSHLYQKSLKGMPFTTIQPPHSITVFNGSPRGEKG
                     SNTLILLNEFTRSFEGNIQLLHLWNGKDMENHVKAFQSAECVWLGFPLYTDCMPSIVT
                     RFIDALQPLLKRSINPPIGFLVQSGFAESLHSRYVERYLEKLARRLGSPYLGTIVKGG
                     GEGIHVMPPQANRKLFQNLQHLGASFKSNGKLDERVLSQIAKPERIPTLIIPPVKKAM
                     RIPLFNSYFDGQLQKNGAYEKRFDRPFLEER"
     gene            14139..14573
                     /locus_tag="PLL88_07225"
     CDS             14139..14573
                     /locus_tag="PLL88_07225"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOJ01396.1"
                     /translation="MTTRVLFLCTGNSARSQMAEAFLRKYGGEQFEAFSAGLEPKEIH
                     PLTIQVMNEKGIDISTQRSKGIETYLGKAHFQYLITVCDEADKNCPTIFPGVNQRMHW
                     SFEDPAAYQGTEEEKLEKFRAIRDQIEKRVKDWVAGLDQSEA"
BASE COUNT         3898 a         3636 c         3802 g         3522 t
ORIGIN      
        1 ccatctcggc tattcttgga gcgaggcgca cgaggatgcg gagcttctgg aacacattac
       61 tccgctacgc ttgacctccc ggctcgataa attcttgaat tacccggctt actgtcccca
      121 tggcagcgcc atcccgcaca ccgacgggca aatcgacagc gttcctctgc aaaccctgaa
      181 tctgctttca gcgggagaaa cctcgcatat ccgcaaggta acggaggaaa gggagctgct
      241 cgattatctt caaaacctgg agatagaaat cggaagcgcc atcacggttg tttcagtcgg
      301 cgcatacgaa ggtccgctga cgcttgccct ggacggcggc aagcgcgttc aaatcagtta
      361 taaagccgcc tgccagatct acgtcgatca ggcagcgcag cataacacca caatcacatc
      421 acaataagga gattcatcat gaataaaaaa agtcaacatt tttctggaat cgcgctgagt
      481 tgcgtgcttt tgcttgccgc ttgcgcacag cccgcggcga atccggagcc cacggaagat
      541 acgttaaacg tggtagcaac cacgaccatg cttgcagatt tgtccagagt catcggtgga
      601 gagtacgtca ccgtgagcgg attgatggga cccggcatcg atccccattt atatcaagcc
      661 agtgcgggcg atgtaacacg gatgcaggag gcggatgttg tggtctataa cgggctgcac
      721 cttgagggaa aaatgggcga gatttttgaa aacctgtccg gtcaggggca cgcggtgatc
      781 tgcatcgagg acggattaga cgaatccaag ctcctcgcat gggagggcgg cggctcggtg
      841 cacgatccgc atatctggtt tgatgttgca atctggaaac aagccgcgca aattgtcgca
      901 gagggctttg ccaacgccga cccggctcat tcggatgcgt acaagggaaa cctggagcaa
      961 tatctcaagg agcttgatga actggataca tacgttcgcg acagagtttc agaggtagcg
     1021 gaagggcagc gggtgctggt cacagcgcac gacgcgttca attactttgg caaagcctat
     1081 ggctttgagg tgcggggact gcagggcatc agtacggagg ctgaggcggg caccgccgac
     1141 gtaagtgcac tggctgattt catcgtggag cggcagatca aagcgatctt cgtggaatcc
     1201 tccgtgccgc ccaagaccat tgaggcgctt caagcagccg taaaggcaaa gggttttgat
     1261 gtgtccatcg gcggagaact atactccgat tcattgggtg gagaaggttc cggtgctgaa
     1321 agctacatcc tcaccgtcaa agccaacatc gacaccatcg tggaggctct aaaatgacgc
     1381 aggtggtcga catggagaga agcgcacaag gttatgcagt ggaggtggag gacctcaccg
     1441 tcgcttacga cacaaaaccc gtcttatggg atattgacct gaagatcccc aagggtaagc
     1501 tgatggcggt ggtcggaccg aatggtgcag gcaagacaac attgatcaaa gcgatgctgg
     1561 ggctgctgac gccggtctcc ggatgcatcc gttttatcaa cggcagcgac aatgtgcgtt
     1621 cgctgaaaaa ccgtattggc tacgttccac aaagcggcag cgtggactgg gattttcccg
     1681 caaccgtgca ggatgtcgtt ctcatgggac gctacggcaa actcggttgg atcaaacgcc
     1741 cccgcaaggc agattatgaa ttaacgctac aaatgctcaa gaaggtcggt atggacgcat
     1801 atgcttaccg ccagatcaac caactttccg gagggcaaca acaacgggtt tttctggctc
     1861 gtgcgttggt gcaggaagcg gatgtctact tcatggatga accgttcaaa ggggtagacg
     1921 cacagaccga aaaagccatc gtcctgctct taaaagagct gaaagggcag ggaaaaaccg
     1981 tcgtggtcgt acaccacgat cttcagacgg tggcagatta tttcgactgg gtgacgctga
     2041 tcaatctgag ggtgatcgcc agcggaccgg tggaggaggt tttctatgag gaaaacctga
     2101 agaaaacata ccggagcacc ggcacacttc taaggagcgc gacgtgatat gaacgcgtgg
     2161 atttctttgt tcaacgacta caccttccag acggtggcac taggctcggc tcttttgggg
     2221 cttatcagcg gcgtgttagg gagctttgcc gtactgcgca agcaaagcct tttgggtgat
     2281 ggcatttctc attccgcact acccggcgtc gtcatggcgt tcatcctgct cggcagcaag
     2341 aacaccgaaa cgttgctgtt gggggcgctc atttccggtt tgacggctac acttttcatc
     2401 gtcggcattg tcaggcacac gcgcatcaaa ttcgacagcg ccctggctct cgtcatgtcg
     2461 gtgttttttg ggttggggct ggtacttctg acctatgtac agaagattcc caactctaac
     2521 caggcagggc ttagccgctt tatttttggg caggcgtcga cactgctgca acgggacatt
     2581 attctgatgg ctgtatgcgg cgcggtactg ctggcacttg tcattctgtt ctggaaggag
     2641 ttcaagctct ctacctttga cagcgacttt gcacaaagcc ttgacttctc gcctaaaaag
     2701 ctgaacctgc tgctatcgtt catgatcgtg ctgagcatca tcatcgggtt gcagacggta
     2761 ggggtcatcc tgatgagcgc catgctgatt gccccggctg tcgcagcgcg ccagtggacc
     2821 aataagttgt gggggatggt cctgctttct gccattttcg gcgtggtgtc cggcattgtc
     2881 ggaacagcga tcagttccct ggtggcaaag ctgccgacag gaccggtgat cgtagtgtgc
     2941 atcagcgtga tcgttatggt cagcgttttg tttgcgccgg gaaggggtat tctgcacaga
     3001 ctgtatcaac gccggaaaaa tagacttcta ttgaagatgg aaggaggtga gcctaatgtc
     3061 cccacaactt gaaatacaac ttattgccgt gattgtggcg gtgacctgcg cacttcctgg
     3121 tgcgttcctg gtgctgcgca aaatgtccat gatgtccgat tcgatcaccc atacaatcct
     3181 gctcggcatc gtactggcgt tttttatgac gcatgaccta tcctctccgt tgctgatact
     3241 cggtgcagcg ttgatgggcg ttgtaaccgt ttggttgacc gaattgttag gtcgcacgcg
     3301 acttttggct gaagatgcgg ctatcggcat tgtgttccca ctgctttttt ccatcgcgat
     3361 cattcttatc acgcgctatg cgggctcggt gcacctggac accgattcgg tgctgctggg
     3421 cgagcttgcc tttgcgccat tcgatcggat ggttgtagcg ggcatggata tcggcgcaaa
     3481 agccatctac acaacaggcg cgcttttcct catcaatctg gcggtcatca ttattttctt
     3541 caaggagttg aaggtcgcaa ccttcgatcc catgcttgcc gccgtactgg gctttgcgcc
     3601 tgcgctggtg cattacggac tgatgacact ggtttcgctc accgcggtga gcgcgttcca
     3661 ggcggtgggt tccatattgg tggtagcgtt catgatcggt ccgccggtca cagcgtatct
     3721 gctcaccgac gatttgaagt ggatgctgat cttaagcggg ctgatcggcg caacaaacgg
     3781 gatcttgggt taccaagcag ccgctttgct ggacgtgtcc atcgcgggca gcatggcagt
     3841 gatgacaggg atcgttttcc tgttggtgtt catttttgcc ccacacagag ggctcgtcag
     3901 cgccctgcgt cgacgaagga atcagaaaat acaattcgca aaaatgacgc tcctgttcca
     3961 cctttacaat catgaaggca gcgagaacga agcgagtgaa gccggcattg acgacataca
     4021 cactcatttg cgctgggatg aagcttttac aaagagtatc ctggcgctct tgaaaaaaga
     4081 ggattgcata aagtttacag atggcgtgat caaactgacc gatcaagggc gttcaaacag
     4141 cctccgcaac tatgaggcac tgttttcaga ataaagctgc ttcaggcagg ttcaaaatac
     4201 cgacctttga ttcataaagg tcggtatttt cttgcagagc ctgttttcca tgcaatacta
     4261 tcctctttca cttcgtgcaa aatgtacatc cctagaaaaa accttccgcc catggttcct
     4321 cagcagatgg cagggctgcc tgcactttta tcacgaggcg tttctgtaca tacagacaac
     4381 gccgtatcac gaatatccca cctttataaa aaggcgcgca cgttttgacc agcatacttt
     4441 ctgtgatatt ctatatgcag gatatcacag agaaaataaa ctttaacgta tagggaaatc
     4501 gactcgcctt ttcttgcttt actgaaacgg agggattgag atgagcgatt ggaaaacggt
     4561 actcaaagcg gatccgacaa actggctgct ggaggaagag aacccatctg tgcgctattt
     4621 cacgttaaaa gatattttag gcagggcgga ggatgacaag gcagtttgca ctgccagaca
     4681 agatgtgatg caaaccggca tggtacctat gattcttgat aagcaacgtg aagccgcgta
     4741 ccttcagagc tattcccgat tttataccta caaatataaa gggcttgtct ggtcactgat
     4801 cgtgctcaca gaattggggg ctgaagcaaa tgcgcaaatc aaagagcact gcgagtacct
     4861 gttcgaaaat tcgcaagaga ggcaagacgg cggtttttcg caaaatacag ccgcgagggc
     4921 tggcggcggc aggattaccg aagtgatccc gtgcctgagc ggaaatatgg tatggagcct
     4981 gatccgcttc ggctatctcg acgatacgag gctgcaaaag ggcatcgatt ggatcacccg
     5041 atatatgcgg tttaacgacg gcgtagcgga tgacccacaa gtgccgccgt atgatcgtta
     5101 tgaaatatgt tggggggcgc acacctgtca tatgggtgta gtcaaggcgc tcaaagcatt
     5161 gagtgccgtg ccggtggaaa gaagaaccaa agaaataaac gatacgatac aaaaggcagc
     5221 cgagtttcta ttaattcacc acatctataa acgcagccac aatttaaaca ggacgtccaa
     5281 gcccggctgg ctcaaattcg gctttccttt aatgtatcag acggacgtgc tggaaatatt
     5341 ggatatcctg accgaactcg gcattgaaga cagccgcatg gatgaagcaa tcaacatcgt
     5401 cattgcaaag caagacgata cgggtagatg gaaaacggag aacacctaca acagcgaccg
     5461 actgcttatt ccaatggggc aaaaagacga gcagagcaaa tggatcacac tcagagctat
     5521 gcgggtgttg aaaagatatc aaggcagata ggaatgatca ttcttcatca tgattgatgg
     5581 ctgaaaaagc tcaacgccgt caaattgtga aaaaagtacg cccttttgga aattgcacgc
     5641 gctaaaaaac gaaacggatt gcatggcaga atatcatctt tgcactgggc gtgaaggtgt
     5701 tgcggtgaaa aaggacaata tgatccttag gcttttttct caatagcagg caagtttttt
     5761 cttattatga aaaagcatgc ggtttgcgtg cagcgggaga aaagtgccgc ttttctggca
     5821 catttttacc catataatca agttatcaag tattttcaac ccaaggaggt cagtagacag
     5881 caatgaccgt agcgcaaccg tatcaatctg atgttgatgc gattctggca aagcgatatg
     5941 acaacggcgc cgattattgg accacacccg acaggcgatt gctcaaagga ggaccgtttt
     6001 ctaccctcga atgcgcgttt atgctgctgg aattgggaat ggaaccctcc gatcttttat
     6061 tgaaagagac tgccgacctg ttcttcagtg cgtggcggga agatggaagg ttcaagctgt
     6121 cgccgcaagg cgcaatatat ccctgctata caattaacgc caccaacgtg ctttgccatt
     6181 taggatatgc tgctgacagc aggctccaga gaactattga ccacctttta gaaatccagc
     6241 acagcgacgg cggctggcgt tgcaacaaat tcagtttcgg cagaggtccg gaaacagagt
     6301 tctcaaatcc cggtccaacg ttggcggctc tcaacgcttt tcgattcaca gatctgctca
     6361 ataaggaacc agcccttgac agagccgtag aattcttgct cgaccattgg acaacgcgcc
     6421 tgcctctcgg tccctgccat tatgggatcg gcactttgtt catgcaggtt gaatacccct
     6481 tcgccaatta caaccttttc gtctatgtct acgttctctc cttctacgac agcgccagga
     6541 gagataagcg atttctggat gcgtttgggg ctttggagtc caaggtcgtt gacggcaaga
     6601 tcgtcgtgga gcgggtcaac cggaaattag cagggctgac cttctgtaaa aaaggggaac
     6661 caagcgactt gggaaccatg cgataccatg agatactgaa aaatcttagc tgcgtcaaaa
     6721 cgtgaagcag cgctcacctt cttggacgta cacccgaaaa aaatgaccgc gatgtttccc
     6781 cgcctttcgt aaaacagtgc cacccctgta agaagcccag gcggcgctgt tttctatcag
     6841 cagacgcgat ttttgcgttc acggcttaca ccttgcttat ggtggacttg ttgttgaaaa
     6901 gggagtctgc ttttcctcat tttgtataat cctcttcaac atccactggc agtggaggag
     6961 aacgaaccaa catcgtattc ttccaatagt tagtgtatac taactcaaac cggctccacg
     7021 ttgccgtaga gaggaaatgg atagaaagtg ctcaaaaaat tctttttgaa tacgcgaagc
     7081 cctgagggca tgggtggcgt gatcatgttg tggttgatga atgtggggca tagttccatg
     7141 tcgcaatggg ggttgaaaca gcttgctgta aagcccgatg acaatatttt agacatcggg
     7201 tgcggcggcg gcgcgaacat cgctcgcatg ctcgacatga ctccgcatgg aaaggtgtgc
     7261 ggggtggatt atgctgctct aagcgtagaa aaatccatac agcgcaaccg aaaagccatc
     7321 gctgccaagc gtgcggaaat aaaacagggg gatgtgtcgc aaatacccta tgcggatagc
     7381 acatttgata tcgttaccgc ttttgaaacc gtctatttct ggcaagattt tcccaacgac
     7441 ctgaaagaag tggcgcgggt gttaaagccc ggcggaacct tttttatttg caacgaagcg
     7501 gtacgtttgg agggggaaga tccgccgcac aagggattca tcaaaatgtt ggacctgaac
     7561 atgtattccg caaacgattt caggcaaacg ctgctggcgg caggatttga aagcgtgaac
     7621 gtcattctag cgaagcacaa gaacatgata tgcgtcatgg cgaaaaaacc gggagcacga
     7681 aaaacatctc caaccgaaga cgaccttttc tctgaaacgt ctctttaagc agtttttttg
     7741 ggtcagcgcg gtttttcctg ccctcaagaa gccgatagag cgcttctggt accatgaaac
     7801 accccctccc gacacaaaca tcgacgcggg ataatgcccg atcaggtggg gttacaatct
     7861 ttgcagtacc caaacacctc caaattatga cccatcacat gaaaatcttc atcttccagt
     7921 tttggcacaa atttctccat cgggcaatta tccatcggga tgatcttatg acagctcatg
     7981 cagaccgcat aatgcttatg cttgaaacgg ttgagctcat acactgccat ttcattattc
     8041 atcacgttgg ttttaatcac cacgcctttc ctaacaaaaa gttccaggat gcgatagacg
     8101 gtagacagcc aagcgtcatc tccgccttct tccacctggg aaaatatgtc gggtgcgctc
     8161 aagggcttct cagaattttc gagtatcgag agcacacttt cacgctgcct ggttcttttg
     8221 acccctgcag gccagttatt ttgcacttct ttcaatctgt tcaccttatc cactggctca
     8281 aaaagagcct gctaaccctt tcttgcacac ggcacatcct gccatatcta ccaaccgcgc
     8341 cattttgtgg aatctgcacc tttacatatt ccccgcagtg caccgcggtt ctttttccgt
     8401 gacgcgcaaa tgcgtattat tcgcatttga tttcattata gaatggcggt tccaagaagt
     8461 caagcattgt gcgcggatgt cggcacagac gcatcatttg ctgcgggcgg cgctgatgct
     8521 cgattgcagc cttttggcga tcaaaaccaa aacaaggacg gcaacggaaa caagggcggt
     8581 aaagcccccg ggagcgacat taagataata cgaaacaaac agccccaaca tgatatcgat
     8641 tacgctgaaa acaattgaaa agatcaaagt gagtttaaat cctttcccaa gctgcagtgc
     8701 cgtggcaacg ggaagggcaa tcattgaact aagcaccagc acacccacaa ttcttatgga
     8761 tacggatatg gcggaggcaa ccagaacgaa aaaaacatag ttaatcagcc ccacttttac
     8821 gcccgccact ttcgcggcat cttcatcata ggcgatataa atcatttgat ggtatagaag
     8881 aataagggtt aaaacggaaa tcgcactgag gatcaacacc agcaccacat ccaagcgggt
     8941 taccgtcaga atactaccaa acataaagga atctgcattg gcacgaagct tcccggaact
     9001 gatgatggta atcgcgatac ctacactcag tgaaagcaca atggtgagaa tcaaatcggt
     9061 atattttttg aaatagctac gcagaaactc aatcagcgcg ccacaaatgg aagtgaagat
     9121 aaatgcgccg agtatgggat tctggttagc catcagcccg attgtgatgc cagccagcga
     9181 ggcatgcgat agtgtgtcac ctatcataga atagcggcga agaaccaaaa agatgccgat
     9241 gcaggggcat agaatcgcaa taaacatgga aacgaaaatc gcattttgca taaaaccgta
     9301 attaagcatg ggtatcgtcc ttcccgcggt acttcaagaa ctcatcggca tagcgccggg
     9361 gggtgcagag atgcccctgc ccgcccatca gatggtaaat caatgttgaa tttgaaatga
     9421 cggcatccag attgtgttca acggaaacaa tggtgatacc gttttctctg ttgatctttt
     9481 tcaaaaaatc ataaatttct ttttggctgt tggtatcgac cccgttcaag ggttcatcca
     9541 gaatcagcaa atcggggtct cccatcaatg ctcgcgcaat cagtattctc tgattctgcc
     9601 ctgcggagag ggcgcccatc aacactcctg taaagttggt cattccaacc cgctcaagac
     9661 tttcggtaat tacgcccctg tttttgatct tcaacaattt gcggtaagaa ttcagtgcct
     9721 cataaaccgt gatgggaaaa ttagcattag agaaatcgtt tttttgcggg acatacccga
     9781 ctctttttgc ttgagacagg atgctcccgc tggtgggttt tatgaacctc aatatcaagc
     9841 gcagaagcgt actctttccg ctgccgtttt cgcccaccac cgacatatag tcgccagtgc
     9901 gtattttcag attgatccca tccaacacat aaggcggcga acctgtatag gaaaagaata
     9961 ctttctctgc tttgatcatg aacaaaatcc tttctgactg caaactataa accatttttg
    10021 ttgcaaatgc catacattcg cacttgacaa aaatatccct gcacactatc atgtattata
    10081 aatgcaaatg cttctcattt gcaaatagat tttcggaggt tggagaagat gttaaaaaaa
    10141 tggaccgctt ttttgctatg cttgattgtc attgtgagct tttcggcgtg cagcagcaat
    10201 gatcatccgg cagatgaaac ggaagaaagc aaactcaacg ttgtcgtaac ctttaacgcg
    10261 atgaaagagt tcgtcgcagc cgtgggaaag gacaaggtag agattttaac gatcatccct
    10321 gatggcgtgg aaccgcacga ttttgaacca aaggcacagg acctggttgc ccttagcaca
    10381 gccgaggttt ttgtgtacag cggactggga atggaaacat gggtggagga agcgatcaac
    10441 gccgccaaca atgcaaatct gattatcgtg gaagcagcga agggtgccga cgcaattgag
    10501 aacacggagc ctgaggaagt tgaagaacac gggcaatatg acccccacat ctggctcagc
    10561 ctgaaagggg ctgaattgga agcgaaagcc atcaaagacg cactagttca ggcagaccca
    10621 gccaacaaag actactatga acagaactgc gctgattttg tggcacagct cgaagacctt
    10681 ttcaacgaat acaacgagaa attccagtct gtggagaaaa agagctttgt aacgggacat
    10741 gcagcttttg cctacctatg ccgagacttc ggcttggaac aaaacagtgt ggaagacgtt
    10801 ttcgctgagg gagaaccgag cgcgcggcaa ctggctgagc ttgttgaata ttgcagagag
    10861 cacaacgtga cgacaatttt tgccgaagaa atggcaagcc cggatgtttc ccaaacgctg
    10921 gcgaacgaag tcggcgcaac ggtagaaacc atctatacga ttgagagcaa cgaggacgat
    10981 atgacctacc tggaacgcat gagggacaac ctgtctaaga tttatgagag tctgacaaaa
    11041 taaagactat acatttcgtt gagaaagcgg agatttgtca tcagtgcgtg gagatgagcg
    11101 cgatggagtc tgaaagacca taataatctg cgcttctcat aacatccaca gcgatgagtt
    11161 cgagccgttc cgtacgcctg aaggacccgg gaatccatgc cgggtctttt tttatctccc
    11221 tatcgataaa aatagcaatt tctcttgtat tttaatatag tagatgtata atatatagga
    11281 tatatcttat ataggagttg catagtatgt ctctcgaaca cgccattctg ggttttctca
    11341 actaccagcc atacacgggg tatgacctga agaagatctt cgacgcctcc gtgcaacatt
    11401 tctggtctgc agaccagagc cagatctacc gcacgttggg caaactggaa gaacaggagc
    11461 tggtaaaaat ggaaaaagta gcacaggagg accgcccgga tcgcaaggtg tatcacatca
    11521 ccccaaaggg aaaagaggaa ttgttgcgct ggctggctgg accgccactc atgggagcac
    11581 cacacagttc gcccctggtg caagtatttt ttggcgggct attgccagac gaagaactgc
    11641 tgatcaagtt cgaagggttc gcgcaaatga tgcgtgctgt gctggcacag tatgagcagg
    11701 tgccagaaag catcgagacc ttcaaaaaag aaatcccttc cagacgcgag cattacttct
    11761 ggatgctgac attggacaac ggcatccgca atatgcgcgc caacctggaa tgggtcgaga
    11821 atatcattca gcagataaaa aatggcgaag taccacaaga acaggaataa aaaatgatca
    11881 aacaaatccc tttgcggcaa cgtgtgcgga aatttatcct gatcgcggtc tttttctctt
    11941 ttccgatcac catgaactac ctctccccct acctcatcct tgaaggggct tcacaaggta
    12001 tcgtcagcgg tagtttgatc accttcacgt tccttttcat ctcttccctt ttcctgggtc
    12061 gcgcatggtg cggctgggtg tgcccgggtg ggacaatcca ggacatcgtg gaaccggtca
    12121 acaacaagct gatccatggg aaaagaatcg attgggtcaa atggctagtc tggatcccct
    12181 gggtgagcct gatcggctgg ctattcatcc ggtcgggcgg catcagacag atcaatccac
    12241 tgttcggaac agaaggcggc atttcggtgg ctggctcagc ggagcgccct atcatgttcg
    12301 cctatttcat ttactacatg gtgatcgtgc tgttcagtgg actggcgctg atcgttggca
    12361 ggcgcgccgg atgccacagc atctgctgga tggcaccttt catgatgatc gggcgctggc
    12421 tccgcaaccg gattggttgg attgctttgc gcttgcaagc cacacccagc gcctgcgtag
    12481 attgcaagat ctgcaacggg cactgcccca tgagtttacc cgttaaccaa atggtactgg
    12541 acaataaaat ggaaaacgca gagtgcattt tgtgtggttc ctgcgtagat ttctgcccca
    12601 aggacgccat ccgatacaca ttcagcgcta acagataaga gaaagcgaac catgaagatc
    12661 accatattaa acagttcaca aaccagcgct gcgctggatg gctatatcaa cacattggtc
    12721 aatctactgg aaagcaaagg gcacgaggtc cagaaccttt tgctgcgcga gatgaatctg
    12781 cgatcctgca ggggctgttt ccattgctgg gtgaaaacgc cgggatactg cgtgctgaag
    12841 gatgattccg acttgctcga ccgtacggtc atcagctccg attttgtgct atgggcttcg
    12901 cccatggtgc tgggttttcc caatgagttc ttcaaacgcg ccctcgataa gcatctgccg
    12961 ctgttgctgc ccaacatgga gatcgcggag ggtgagatgc accatgtggc gcgctaccca
    13021 cactacccgc gcattggcat gttggtagaa aaggaacgcg caacggatga ggaggatgtg
    13081 cgtattctga ccgatatttt cgcgcggaca gccttgaatt tcaaaacccg tctggaattc
    13141 ctcgcgacca cccaggaagc gccagagcag atcagcgagc agatcttgca cccgcaggaa
    13201 ggatcccatc tctatcagaa atctctgaaa ggcatgcctt tcaccaccat ccagccaccc
    13261 cacagcatca cagtgttcaa cggctctcca cgcggcgaaa aaggcagcaa cacgctgatc
    13321 ctgttgaacg aattcacccg cagtttcgag gggaatatcc agctcctcca cctgtggaac
    13381 gggaaggata tggagaacca tgtgaaagct ttccagtccg cggaatgcgt gtggctggga
    13441 ttcccactct acacagactg catgccaagc atcgtcacac gcttcatcga cgctctgcag
    13501 cccctgctca agcgatcgat caacccaccc atcggttttc tggtgcaatc aggctttgcg
    13561 gaaagtctgc attcgcgcta cgtggaacgc tacctggaga aactagccag gcgcttgggt
    13621 tccccctacc tgggcaccat cgtcaagggc ggtggggagg gcattcacgt catgccgccc
    13681 caggcgaatc gcaaactgtt tcaaaatctg cagcacctgg gggcatcgtt caagagcaac
    13741 ggcaagctgg atgaaagagt attgagccag atcgccaagc cggagcgcat tcctaccctg
    13801 atcatccctc ccgtgaagaa agctatgcgc atacccttgt tcaattccta cttcgatggg
    13861 caactgcaaa aaaatggcgc ttatgaaaaa cgtttcgaca gacctttcct ggaggaacgt
    13921 tgatattctt aagaactgtt tattaccata cgctgttctt aactcgccct atttcgaaga
    13981 tcgcgacttt ggatatacac acgacggaaa gcatagcggt tgccatgctt ttttatgcac
    14041 aaatagcatt tgcgctatcc taactgactt ttaatatgac gttaatctca caaccataca
    14101 atatcatcca tactatggat agtatttcag gagataagat gaccacgcgc gttctctttt
    14161 tatgcacagg caattccgcc cgcagccaga tggcagaagc atttttgcgc aaatacggcg
    14221 gagagcagtt cgaggcgttc agcgccgggt tggaaccgaa agagatccac cccttaacca
    14281 tacaggtgat gaacgagaag gggattgaca tctctaccca acgctcgaag ggaatagaaa
    14341 cctacttggg caaagcgcat ttccagtacc tgatcacggt atgcgacgag gcggacaaga
    14401 actgccccac catttttccg ggagtgaatc agcgcatgca ctggtcattc gaggacccgg
    14461 cagcttacca gggaacagaa gaagaaaagc tggaaaaatt ccgtgccatt cgcgaccaga
    14521 tcgaaaaacg ggtaaaggat tgggtagcag gtctcgatca atctgaagca taaaaagaag
    14581 aatcacccag ttctatatct tatttatggt tgttctttgc gatgtgtatg gtatttcaac
    14641 acgaaatgga gagattctct ggtctatgca ctgcgcaaag ggagtttcaa tggtgcgttg
    14701 agaacgcatc cgtctctttc actcctcaat agatttggcg cgtcaggaac gcgccttgca
    14761 aactgttttc ccgcaagcag ggagtcgtgg tgggttagaa acccaccctt gtatctttaa
    14821 agacagatat gttagaaagg cagaaggcac cggttgtg
//
LOCUS       DAOSDS010000052        14566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1641_length_14566_cov_6.096203, whole genome shotgun sequence.
ACCESSION   DAOSDS010000052 DAOSDS010000000
VERSION     DAOSDS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1641_length_14566_cov_6.096203"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1261)
                     /locus_tag="PLL88_07230"
     CDS             complement(107..1261)
                     /locus_tag="PLL88_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOJ01397.1"
                     /translation="MQANKQEFEEREQLRKARIRVGSNGVTTPFILDYLGRSLPAMLD
                     FLGFSDAELYLKKEKSPVLMRVLHYGESKEFFWKDEQYELGKNTLGKIAQMVEPCVLD
                     LSDETPAELNKRIRQIGFSMLACFPIRAESGALGVLSLGDKKIRKLDEKVEDVLMENA
                     AMIGTVVFHERKSLEAKRSIVMEERERIGMDLHDGIIQSLYGVGLSLQNAVLQFDNNE
                     QEGKQHIDDALNAINEAIRDIRAYILDLRPRQLRNQDLYMGIESLIREFRANTLVDVE
                     FEKDIAYIERVSDENSEILFHVCQEALANVAKHARATKVNVRLWETPQRIMLKVNDDG
                     VGFDLSKINQRLGHGLSNMNTRVETVGGGIEIISIRHQGTTVLAWVPNNL"
     gene            1377..2003
                     /locus_tag="PLL88_07235"
     CDS             1377..2003
                     /locus_tag="PLL88_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOJ01398.1"
                     /translation="MPDRKEFEEKMQQEQRLPPGQSLTLKFPVLHYGAVPLFVPQAWT
                     FRVFGEVKEEKTWSWEEFQQLPRTQVCYDLHCVTRWSKFDTHWEGVQVKTLLEEKIII
                     PNRGAKYVIQHVENGFTANLPLEVIASELFLMATHFEGQPISPEHGYPLRGLVGAIPG
                     KRSDRDVYLWKGAKWLRGLEFSSVDKPGFWEQAGYHNEASIWKEQRFG"
     gene            complement(2029..2766)
                     /locus_tag="PLL88_07240"
     CDS             complement(2029..2766)
                     /locus_tag="PLL88_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01399.1"
                     /translation="MKFLLFASRNRKEILRDPLNLAFGIGFPLIVLLLLSVIQANIPV
                     SLFEIGKLAPGIAVFGLSFIALFSGTLIAKDRSTSFLMRLFASPLSASDFILGYTLPF
                     LPIAMAQSALCFGVSFFLGLQMNANILLTLLVLFPVASLFIGIGLLAGSVFTDKQVGG
                     VCGALLTNLSAWLSGTWFDLNLVGGAFKTIAYVLPFVHAVDAAKAAMAGDTASIFPSL
                     WWVIGYAVVILTMAIFVFKKKMHSDQI"
     gene            complement(2763..3497)
                     /locus_tag="PLL88_07245"
     CDS             complement(2763..3497)
                     /locus_tag="PLL88_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01400.1"
                     /translation="MIAIQTKALTKRYKDRLAVNALDLSIEQGEFFALLGVNGAGKTT
                     TIKMLSCLTRPTSGDALLLGDSVLSKPDAVKKKINISPQETAVAGNLSVKENLELVAG
                     IYGSDAKTAQSKTNEMLSTFGLSEIAQHKAKTISGGMQRRLSIAMALISHPQILFLDE
                     PTLGLDVFARRELWSAIEKLKGEITIILTTHYMEEAEALSDRIGIMTRGRLQDVGTSA
                     ELIAKTGTKTFEDAFIALASESEVDR"
     gene            complement(3494..3721)
                     /locus_tag="PLL88_07250"
     CDS             complement(3494..3721)
                     /locus_tag="PLL88_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOJ01401.1"
                     /translation="MTVPKGKHIFGIVKVGEKGQVVIPKEARKIFDIKPGDNLLVFGD
                     EAQGIALAKSEEFLQFAEEIFNAKRIDEDGE"
     gene            complement(3928..4701)
                     /locus_tag="PLL88_07255"
     CDS             complement(3928..4701)
                     /locus_tag="PLL88_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ01402.1"
                     /translation="MIKKQIRVIGVFFICLLVMIAFVTTTGSYPLPQAYYYTPTPQAD
                     GRILYTVKENDTCISIALLNGISEGELRNLNNLQSDDCNYLQVGQQLLIGVLEEPTAT
                     TEALQTTLEPTPTPIKGNGTICIYLFNDENGNAMAEDAEPPLAGGAVSVRDRLSTINL
                     TGITDDSGEPICFEDIPEGDYDLSVAIPEGYNPTTVMNYTLNLLPGDVSIVDFGAQPS
                     SRALPIFGDEKPSIFMGLLGILFIGAGMGLWFYVRKLPK"
     gene            4832..5254
                     /gene="ssb"
                     /locus_tag="PLL88_07260"
     CDS             4832..5254
                     /gene="ssb"
                     /locus_tag="PLL88_07260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ01403.1"
                     /translation="MSFHTLVLVGRLGQDPEMRYMPNGQAVTNFSVATDRTYKNTNGE
                     QVRVTTWFRVSVWGKQAESCNQYLNKGSMVLIEGTLNPDKETGRPRIWTSADGRSGTS
                     YEVNAQTVRFLSTRGSSSEAPVESDGGMPDLDENDIPF"
     gene            5263..5910
                     /locus_tag="PLL88_07265"
     CDS             5263..5910
                     /locus_tag="PLL88_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HOJ01404.1"
                     /translation="MNSDFSRLSDQCHQVLEACDKAREGALEQTRLLTRNAANAIRAV
                     HRQEFTLAEQLLTEARAISDALKANLEPYPELYFSGYTQDSIKEYVEAQLTLDLIQGN
                     PLPLPAELNVEVSTYLRGLSETIGELRRKCLDILRQGYSEEAERLLDLMDEIYALLVT
                     VDYPDAVTHGLRRQTDLARGIIERTRADLTTSLRQECLQKAIVSLENKLEENGKE"
     gene            5897..6148
                     /locus_tag="PLL88_07270"
     CDS             5897..6148
                     /locus_tag="PLL88_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01405.1"
                     /translation="MARSKRTVRASEIGDYLFCERAWWYVRQGLKNRNQAELAGGLSF
                     HEEHAGEARRIFLSQIAGYLLLAVSVVIFIIVLISGSLS"
     gene            6155..6679
                     /gene="cas4"
                     /locus_tag="PLL88_07275"
     CDS             6155..6679
                     /gene="cas4"
                     /locus_tag="PLL88_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOJ01406.1"
                     /translation="MSNTLLISIASAVLVIAILILVSSANRRKSLGLPSGTIVYSDPG
                     ILGKVDKPLYDAELGLTGKPDYVVERRGRLIPVEVKSGWAPEKPYDSHIMQLASYCWL
                     LERNFRKRPPYGILKYKNRTFAIRYTDELRQNVIDLVDEIRSQKEYGEPDRSHENPNR
                     CERCGFRFDCDQRL"
     gene            6732..7589
                     /locus_tag="PLL88_07280"
     CDS             6732..7589
                     /locus_tag="PLL88_07280"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOJ01407.1"
                     /translation="MNTPELKKIGVAYHPHIAEAVATAQSVESFLLEQGAESVLCCSL
                     KDPQLEEAITSHSIDALTVLGGDGSMLRASQLCAPAGVPITGINLGTFGFLFELERHN
                     WQNHLAHLMQGKYRIEKRMMLQASHYRSNELLGTWQVVNEIVVCRGQQVKPIRMHASV
                     DEYPMASYVADGLIAATATGSTAYALAVNGPIMPPETRNILVVPVAPHLSFDRAVILN
                     EGSYVTITVDTNYDTVFSADGHEPVKMMNHDSVKISASNMDVHFIRFHNAGFFYRNLN
                     RYMKQNPTV"
     gene            7637..8194
                     /locus_tag="PLL88_07285"
     CDS             7637..8194
                     /locus_tag="PLL88_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01408.1"
                     /translation="MNQQNIEPNQSDTLTCYRHPERETLLRCNQCDRPICSSCAIRTP
                     TGYRCPECVRSQEKKFDTAQLPDYILACLLSAGIAFFGSYVARFLGFFTLLIAPVVSM
                     IISEGILKLTRGRSSTLLTRLVLVSAILGSLPLLLVGLYNMFTYLSLYGLSAIGSFLS
                     TIWQAGYTIIMATTLRARTKGLYIG"
     gene            8200..9951
                     /gene="recN"
                     /locus_tag="PLL88_07290"
     CDS             8200..9951
                     /gene="recN"
                     /locus_tag="PLL88_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOJ01409.1"
                     /translation="MLKELSIRNFAIIPELHLRFKPGLTIFTGETGAGKSIMLDALEV
                     LTGGKTDNSMIRSGADSAYLEAVLSIPTLNRETIVQFLKEQELNENEEEDEIVLSREI
                     RKEGRTSARINGRGVSGTILRALGSFLVDIHGQSEHLSLLKVKEHIHLLDRYCSNQNL
                     LVDYQQTYRAWQQNHRELQELRKAEADAARQQDLLNYQIQEIEAATLQEGEDEELNAE
                     LNRLANAEKLATLCQKANSLLESSEVEASGAIEQLGEVSQALHRIAEIDPSVSELAAQ
                     SDSVLALVDEISRGLQDYVDTVEYNPKRLEQVENRISLINSLKRKYGTSVADIVAFGE
                     KSKKQLDLITHAEERIVELETQEKAILDQLAHKALLLSRNRQNFAQELGKKMEQELAD
                     LHMAGAKFKADLQHEPDENGLLMDGQSRVRFDENGIDQVEFLIAPNPGEGLKPLVKIA
                     SGGETSRLMLALKNVLAEADTIPTLIFDEIDQGIGGRIGAIVGEKLWQLGKNHQVLCV
                     THLPQLAAYGIQHYHVYKHIQDGRTITMVDDIRGERRIHELAAMFGGDTPANCDAAQE
                     ALQSASQRTTALSGAQK"
     gene            10114..10440
                     /locus_tag="PLL88_07295"
     CDS             10114..10440
                     /locus_tag="PLL88_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01410.1"
                     /translation="MKKRFKFLRTLASVFKILGIFLAAISLVCGIILVVLALSNGNFW
                     SLFGLDAATGFTTGIASGILTLVGGLLYGLVVYGFGEMIYVFISIEENTYRTSTFLEE
                     MQKEEQ"
     gene            10519..10595
                     /locus_tag="PLL88_07300"
     tRNA            10519..10595
                     /locus_tag="PLL88_07300"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10553..10555,aa:Arg,seq:gcg)
     gene            10646..11449
                     /gene="surE"
                     /locus_tag="PLL88_07305"
     CDS             10646..11449
                     /gene="surE"
                     /locus_tag="PLL88_07305"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOJ01411.1"
                     /translation="MNHSKKQILLTNDDGIDSPGLWAAAAALSTIGFVTVVAPREKCS
                     AMGRGFGRHEDHKIEVRKMNVNDQEWDVHAVGGTPSETVLHGILEVMPKKPDLVVSGI
                     NYGENVSTDITYSGTVGAAIEAASLGVPALATSYQVNEGDWNSYAQVDFSVAAHFTRF
                     FAERLLSTKLPPDVHLLNLVIPQSATPSTPWQVTRLAPGRYFNPYINRQGGWDDEGHI
                     HARIIIHDDLPEDCDVEVAIKKKMVSVTPVSLDLTSRVDFKALDSLFRI"
     gene            complement(11490..12554)
                     /gene="ychF"
                     /locus_tag="PLL88_07310"
     CDS             complement(11490..12554)
                     /gene="ychF"
                     /locus_tag="PLL88_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOJ01412.1"
                     /translation="MKLGIIGLPQSGKTTIFNALTRASQPTDMSAGKMEIHTGVVDVP
                     DIRVDKLVEFFSARKTVRAKVTYVDIAGLGSGNKGELSGSLLNQLSQMDGFIQVVRCF
                     DDENIPHVLSSIDPARDIASMDAELLLNDQIKIEAKLARLHEDGHKGGRDKGEVERET
                     RLFEKLNESLQQEKPLRTLVLDDEEERMIQGFGFLTRKPMLIVLNLAEGQPVPPLTAS
                     AAAYKLIPLQGKIEMEIAQLPEEEAQLFLQEYGIEEPGLNRMIRESYELLGLLSFFTA
                     GEKEVHAWTVRKGATAPEAAGTIHSDMQQGFIRAEIMAYDDLMVAGSVAELRNRGKLR
                     VEGKNYVIQDGDIVEIRFNI"
     gene            12672..12753
                     /locus_tag="PLL88_07315"
     tRNA            12672..12753
                     /locus_tag="PLL88_07315"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12706..12708,aa:Leu,seq:caa)
     gene            12914..13927
                     /locus_tag="PLL88_07320"
     CDS             12914..13927
                     /locus_tag="PLL88_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ01413.1"
                     /translation="MNKQFKIAIPMAGLGTRMRPHTWSKPKPLIHVAEKTVLDYVLDQ
                     FSSVPDRKNAEYVLIIGPNQREQIEPYIHEKHPEKKVHFVVQEQMRGQSDALYLAREY
                     LHGAVLMAFSDTLIETDLSFLADEKMDGIAWVKPIPDPRRFGVAEVNETGRVARLIEK
                     PRDRKNNLAVVGFYYFKEGRELVSAIQEQFKRDISLKGEYFLVDAINIMIEKGAAFKT
                     NSVEVWLDAGKPDALLETNKYLLDHGSANSEQALSRKGITIVPPVYIHPSATISSCVI
                     GPYASISSACEISNSIIRNSIVEEGAVIKEMILENSLIGRNASIEGRAEQMNIGDDSW
                     IEI"
     gene            14048..>14566
                     /locus_tag="PLL88_07325"
     CDS             14048..>14566
                     /locus_tag="PLL88_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ01414.1"
                     /translation="MQKDYLCRKVRGLHPSIIRQFFDIAATMKDIISLGIGEPDFTTP
                     EPIVNAGMEALRQGQTHYTSNIGMVKLREAIGAHLQQLYGVTYQPDEIIITVGGSEAL
                     YLATTALLENEDEVIIPTPCFVSYQPEVTLAGGIPVEVPTCMEEGFNLNIPAIEKAIT
                     PRTRAVIVNSPNN"
BASE COUNT         3651 a         3791 c         3613 g         3511 t
ORIGIN      
        1 taagaaggtt ccccgcgtaa ggcagccttg tcaagttgga aactgggttc agtgctcaat
       61 aactcttcct gctttcttaa attcagattt tggcgtgcac aatccgtcac aggttattcg
      121 gcacccaggc taacaccgtg gtgccctggt gccggatgga aatgatctcg atgccgccgc
      181 ccaccgtctc tacgcgtgtg ttcatgttcg acaaaccatg ccccagccgc tgattgatct
      241 tggaaagatc aaaacccacc ccgtcatcgt tcaccttgag catgatgcgc tgaggggttt
      301 cccacaggcg cacgttgacc ttggtggctc gggcatgctt ggcaacgttg gctaacgctt
      361 cctggcagac gtggaagagg atttcggagt tctcgtcaga aacacgttca atataagcga
      421 tatccttttc gaattccaca tccaccagcg tgttggcgcg aaattcgcgg atcaggctct
      481 caatgcccat gtacaggtcc tggttgcgca actggcgtgg gcgcaggtcg agaatatagg
      541 cgcggatgtc gcgaatagct tcattgatag cgttcagcgc atcatcgata tgctgtttgc
      601 cctcctgctc attattatcg aattgaagga cagcattctg cagcgaaagt cccaccccat
      661 acagcgattg gatgatacca tcgtgcaggt ccataccgat gcgttcgcgc tcttccatga
      721 cgatcgaacg ctttgcctcc aacgacttgc gctcatgaaa gaccaccgtg ccaatcatgg
      781 ctgcgttttc catgagaaca tcttccacct tctcatcgag cttacggatc ttcttatccc
      841 ccaaactgag cacccccaat gcaccgctct cagcgcgaat gggaaaacaa gccaacatgc
      901 tgaaaccgat ctgcctgatg cgcttgttca attcagcggg ggtttcgtcg cttaaatcaa
      961 gcacgcaggg ttcgaccatt tgagcgatct tgccgagcgt gttttttccc aattcatact
     1021 gctcatcctt ccaaaagaac tccttggatt cgccataatg cagcacgcgc ataagaacgg
     1081 gagacttctc ttttttcagg tagagctccg catccgaaaa gcccagaaaa tccagcatgg
     1141 ctggcagaga acgcccaaga taatccagaa tgaaaggtgt ggtaacacca ttgctgccaa
     1201 ccctgatgcg ggctttgcgc agttgttctc gttcttcaaa ttcttgttta ttggcttgca
     1261 taattctctt tcgaaaggag cttgccccta tcattttact cactttttca gacaaccgtc
     1321 aatttgctgc cggtgcccgc aagcagggga ttggtataat ttggggagag ttttctatgc
     1381 cagatagaaa agaatttgaa gagaagatgc agcaggaaca gcgcctgccg cccgggcagt
     1441 cactgaccct gaaatttccc gtcttgcatt atggggcggt tcctttgttc gtcccacaag
     1501 cgtggacctt tcgcgttttt ggagaggtga aagaagaaaa gacctggagc tgggaagaat
     1561 tccagcagct tccgcgcacg caggtttgtt acgatctgca ctgcgttaca cgctggagca
     1621 agttcgatac tcattgggag ggcgttcagg tcaaaactct gctcgaggaa aaaatcatca
     1681 ttccaaatcg cggagcgaaa tatgtgatcc agcacgtcga gaacggtttc accgccaatc
     1741 tgccgttaga ggtgatcgcc tcggaattat ttttgatggc aacccatttc gaagggcagc
     1801 ccatctcgcc agagcatggc tatccgctgc gcggtctggt gggcgccatc ccgggcaaac
     1861 gcagcgacag ggatgtgtat ctatggaagg gcgccaaatg gctgcgcggg ctggaattca
     1921 gcagcgtcga taagcccggc ttttgggaac aggcaggtta tcacaacgaa gcgagcattt
     1981 ggaaagaaca gcgcttcgga tgatccaatt ccacagaaaa acgattgatc agatctgatc
     2041 gctgtgcatt tttttcttga aaacaaagat tgccatggtc agtataacga ccgcatatcc
     2101 gatcacccac cagagagaag ggaagatgga tgctgtatct cctgccatgg ctgctttggc
     2161 tgcatcgaca gcatggacaa acggaagtac ataagcaatc gttttgaacg cgccacctac
     2221 cagattcagg tcaaaccacg taccgcttaa ccatgcgctc aggttggtca ataaagctcc
     2281 gcatacccct cccacctgtt tgtcggtaaa gacactcccc gccagtaaac cgatgccgat
     2341 aaaaagagat gcgacgggaa acagcacaag caaggttaac aaaatattgg cattcatctg
     2401 caagccaaga aaaaaagata ccccaaagca taacgcgctt tgcgccatcg ctatcggcaa
     2461 gaacggtagt gtatatccaa ggatgaaatc ggacgctgaa agcggcgaag caaacaagcg
     2521 catcagaaac gaagtgcttc tgtctttggc aatcaacgtc cctgaaaaca atgcaataaa
     2581 cgaaagccca aataccgcaa ttccaggagc cagctttccg atttcgaata agctaaccgg
     2641 gatattcgcc tgaatgacgg aaagcaaaag cagcacaatc aatggaaacc cgatgccaaa
     2701 tgccaaattc agagggtctc tcagaatttc tttgcgattg cgggaggcga ataacaagaa
     2761 tttcatcgat ccacctctga ttctgatgca agtgcaataa aagcatcttc aaatgttttg
     2821 gttcctgttt ttgcgatcaa ttctgctgag gtcccaacat cttgcaatct gccccttgtc
     2881 atgatcccga tccgatcaga aagagcttcg gcttcctcca tatagtgtgt tgtaaggatg
     2941 atggtaatct ctcccttcag tttttcgatg gcagaccaca actcacgccg cgcgaagaca
     3001 tcgagcccca gagtgggttc atccaggaac aatatttggg gatgtgagat cagcgccatt
     3061 gcgatgctta atctgcgctg catgccgccg gatattgttt tcgctttatg ttgcgcaatt
     3121 tcactcagcc caaacgttga gagcatttca tttgtcttgc tctgcgcggt tttcgcatca
     3181 gagccataga tacctgcaac cagttcgaga ttttctttaa cggataaatt gccagccaca
     3241 gcggtttcct gcggggatat attgatcttt ttctttaccg catccggttt tgaaagaaca
     3301 ctgtcaccga gcaaaagtgc atcgccactg gtaggtcttg taagacagga aagcatttta
     3361 atggttgtcg tttttcctgc accgttcacg cccaacaatg caaaaaattc gccctgctca
     3421 atagaaagat ccagcgcatt gacagccagt ctgtctttat aacgtttggt cagcgctttg
     3481 gtttgaatgg cgatcattcc ccatcctcat cgattcgctt ggcattgaag atttcctcgg
     3541 cgaattgcag aaattcttca ctttttgcaa gtgcaatacc ttgtgcttca tcaccaaaca
     3601 cgagtaaatt atcacctggc tttatatcga atattttccg tgcttctttg ggaatcacga
     3661 cctgaccttt ttctcccact tttacaatgc caaagatatg ctttcctttt ggaacagtca
     3721 tctttttgtt ctccctttgt attccttgta tgaatagttt aacttattgg aataagtata
     3781 cttctggtaa aatcattaat caaggggaat atatggaata tcaattcaga aacataaaca
     3841 gttttaaaag cttcaccagc gaagcacata aacaaaatgc acctcttgtc ggtgcatttt
     3901 gtttaaccat tggcttgtag tgattgctta tttgggcagt ttgcggacgt agaaccacag
     3961 ccccataccg gctccaatga acaggatgcc cagcaatccc ataaatatgg atggtttctc
     4021 atccccaaaa atgggcagag ctctcgagct gggctgcgcg ccgaaatcga cgatcgaaac
     4081 atcgcccggc agcagattga gggtatagtt catcaccgtg gtcgggttat agccttcggg
     4141 gattgcaaca ctgaggtcgt agtcgccctc cggaatatct tcaaaacaga tcggttcccc
     4201 gctatcatcc gtgattcccg tcagattgat cgtgctcagc cggtcacgca cgctgactgc
     4261 accgcccgcc aggggtggct cggcatcttc cgccatggca ttcccgttct catcattgaa
     4321 aaggtaaatg caaatggtac cgttgccctt gatgggggtg ggagtgggtt ccagggtggt
     4381 ctgcaacgcc tcagtcgtag cggttggttc ttccaaaact ccgatcaaaa gctgctgccc
     4441 gacctgtaaa taattgcaat catcgctctg caggttattg aggttgcgca gctctccttc
     4501 tgagatgccg ttcaacaacg cgatggaaat gcaggtatca ttttctttca ccgtgtagag
     4561 aatacgtcca tccgcctgag gggtaggggt gtagtaatac gcctgtggta gcggatagct
     4621 gccggtggtg gtaacgaaag ctatcatcac cagcaggcag atgaagaata ctccaatcac
     4681 tcgtatttgt tttttgatca tattttcacc tgtgcggtat tataacatcc ctgttttcca
     4741 tcctgctcaa agattttcct gtcaatattc gcatataatt aacgaaagtg aaacgatgaa
     4801 atatcatcaa aaatagattg gaaggaagaa catgtcattt catactctgg tacttgtagg
     4861 tcggctgggg caagatcccg aaatgcgcta catgcccaat ggtcaggcag taacgaattt
     4921 ttcggtggct accgaccgca cctataaaaa cacaaacggc gagcaggtac gtgttaccac
     4981 ctggttccgc gtgtctgtgt ggggaaaaca ggcagaaagc tgcaaccagt atctgaacaa
     5041 gggtagcatg gtactgatcg agggaaccct gaatccggat aaggagaccg gtcgtccgcg
     5101 catctggacc agtgcagatg ggcgctcggg gacttcatat gaggtcaacg cgcaaaccgt
     5161 gcgcttcctt tccacccggg gtagttcttc ggaggcgccc gtggagtccg atggcggaat
     5221 gccagacctg gacgagaacg acatcccgtt ctagagtttt gtatgaattc cgatttttca
     5281 cgattatctg atcagtgcca tcaggttctg gaagcgtgtg acaaagccag ggaaggggcg
     5341 ttagaacaga cacgtctgct cacgcgcaac gctgccaacg caatcagggc tgttcatcgt
     5401 caggagttca cacttgctga acaactgctt accgaagcca gagcgatcag cgacgcattg
     5461 aaagccaacc tggaaccgta tcctgagctg tatttttctg gctacactca ggattccatc
     5521 aaggaatacg tggaagccca attgacgctg gatctcattc agggcaatcc tctcccgttg
     5581 cccgcagaac tcaatgtgga agtatccacc tacctgcgcg gactgtctga aaccatcggg
     5641 gagctgcgtc gcaaatgcct ggacatctta cgccagggtt attctgaaga agcggagcga
     5701 ttgctcgacc tgatggatga aatttacgcg ctgctggtaa cggtggatta tcccgacgcg
     5761 gtcacgcatg ggctgcgccg ccagaccgac ctggcacgcg gcatcatcga acgcacccgc
     5821 gccgatctca ccaccagcct gcgccaagag tgtttgcaga aagctattgt aagtctcgag
     5881 aataagctgg aagagaatgg caaggagtaa aaggaccgtt cgcgcttctg agatcgggga
     5941 ttatctcttc tgcgaacgag cctggtggta cgtgcgccag gggttgaaaa atcgcaacca
     6001 ggcggaactg gcaggtggct tgtctttcca cgaagagcat gccggtgaag ctcgccgcat
     6061 tttcctcagc cagatagccg gataccttct attggcagta tctgtggtga tcttcatcat
     6121 tgtgctcatt tccggttccc tttcctaaga tacgatgtcg aacacactgc tcatttccat
     6181 cgcttccgcc gtcctggtga tcgccatcct tattctcgta agttctgcca acaggagaaa
     6241 gtcccttggt ttgccttcgg gcaccatcgt ctattccgat ccgggcattc tgggaaaggt
     6301 agacaagccg ctctacgacg ccgaactggg tttgaccggg aagccagatt atgtggtcga
     6361 gcgacgcggt cgcctgatac cggtggaggt gaaaagcggc tgggcgcctg aaaaacctta
     6421 tgacagccat atcatgcagt tagccagtta ttgctggctg ctggaacgga atttcagaaa
     6481 acgtcctcct tatggcattt tgaaatacaa gaatcgcacc ttcgccatcc gttataccga
     6541 tgaactgcgc cagaacgtca ttgacctggt tgatgagatc cgttcgcaaa aggaatatgg
     6601 ggaacctgac cgttcacacg aaaacccgaa tcgctgcgaa cgttgcggct ttcgcttcga
     6661 ttgtgaccag cgcctgtaac gcagcccacc tggtccaatt tcgccacatg tcgctataat
     6721 taattcaaat catgaacaca ccagaattga aaaaaatagg ggtggcatat caccctcata
     6781 tcgctgaagc ggtggcaaca gcgcaatcag tggaatcttt tctgctggag cagggtgcag
     6841 agtcggttct gtgctgctct ctcaaagacc ctcagttgga agaagcgatt acatcccata
     6901 gcattgatgc gctgaccgtt ctgggtggag atggcagcat gctgcgcgcc agccagttat
     6961 gtgcaccggc aggcgtcccc atcacaggca tcaacctggg cacctttggc ttcctatttg
     7021 aactggagag gcacaactgg cagaatcacc ttgcgcacct catgcaggga aaataccgca
     7081 tcgaaaagcg catgatgctg caggcttctc attacagaag caatgaattg ttggggacct
     7141 ggcaggtggt gaatgagatc gtggtgtgcc gcgggcagca ggtgaaaccc attcgcatgc
     7201 atgccagtgt ggatgaatac cccatggcaa gttacgtggc agacggtctg atcgccgcca
     7261 ccgccaccgg ctcgacggcg tatgcactgg ctgtgaatgg tcccatcatg ccccccgaaa
     7321 ccaggaacat tctggttgtg ccggtggcac cgcacctttc ttttgaccga gccgtcattc
     7381 ttaacgaagg cagctacgtt accatcacgg tcgacacgaa ttacgatacc gtgttcagcg
     7441 ccgacgggca cgaaccggtc aagatgatga accatgattc ggtgaagatt tctgccagca
     7501 atatggatgt tcacttcatt cgtttccata acgccggttt cttttacagg aacctcaacc
     7561 gctatatgaa acaaaaccca acggtatagc ccatttttca gtattcataa agcgtgcgtt
     7621 tataatgagc agtattatga accaacaaaa tatcgaacca aaccaatccg ataccctcac
     7681 ctgctatcgg caccctgagc gcgaaacgct gctgcgctgt aaccagtgcg accgccccat
     7741 ctgttcttcg tgcgctatcc gcacccccac cggctatcgc tgcccggaat gtgtccgcag
     7801 ccaggagaaa aaatttgata ccgcccagtt gccagattac attctggctt gcctgcttag
     7861 cgcgggcatc gctttcttcg gcagttatgt tgcccgtttt ttgggatttt tcaccctgtt
     7921 gatcgctccg gtggttagca tgatcatcag cgaagggatt ctcaaactca ccagggggcg
     7981 aagctccacc ttattgaccc gcctggtgct ggtcagcgcc atcctcggca gtttgccgct
     8041 gctgttggtt ggtctgtata atatgttcac ttacttgtcg ctttacggct tatctgccat
     8101 cggcagtttt ctttccacca tctggcaagc cgggtatacc atcatcatgg caaccacctt
     8161 gcgcgcccgt accaaaggac tgtacatcgg ttaaaagaaa tgctgaaaga attatcgatc
     8221 cgaaatttcg ccatcatccc ggagctgcat ctgcggttca aacccgggct aaccattttt
     8281 accggtgaaa ccggcgcagg aaagtccatc atgttggatg cgttagaagt gttgaccggc
     8341 ggcaagaccg ataattccat gatccgcagc ggggcagata gcgcgtatct tgaagcggtg
     8401 ctgtctatcc cgaccctgaa tcgagaaact atcgttcaat tcctcaaaga acaggagtta
     8461 aacgagaacg aggaggagga tgagatcgtc ctgagccggg agatccgcaa agaagggcgc
     8521 accagcgcac gcatcaatgg gcggggagtt tcaggcacga tcctgcgcgc actgggcagt
     8581 tttctggtag atattcacgg tcaatcagaa cacttatcat tgctcaaggt aaaagagcat
     8641 atccatcttc tggatcgcta ttgttccaac cagaacctgc tcgtcgatta ccagcaaacc
     8701 taccgcgcct ggcagcaaaa tcatcgcgaa ctgcaggaac tgcgaaaggc tgaagcagat
     8761 gccgcccgcc agcaggacct gttgaactat caaattcagg agatcgaagc cgccaccctg
     8821 caagagggtg aagatgaaga attgaacgcc gaactgaacc ggctggcgaa cgccgaaaaa
     8881 ttagccacgc tctgtcagaa agccaacagc ttactcgaaa gcagcgaagt agaagccagc
     8941 ggggcaatcg aacaactggg tgaggtcagc caggcactgc accgcatcgc cgagatcgat
     9001 ccttcggtat ctgagctagc cgcgcaaagc gattccgtgc tggcgctggt ggatgagatc
     9061 agccgcgggc tgcaagatta cgtcgatacg gtggaataca atccaaaacg cctggaacag
     9121 gttgagaacc gcatttcact catcaattca ttgaaacgga aatatggcac ttctgttgcg
     9181 gacattgtcg cctttggtga gaaatccaaa aaacagctag atctgatcac gcacgccgaa
     9241 gagcgtattg tcgaattgga aacgcaagaa aaagccattc ttgaccagct tgcccataaa
     9301 gccctgctcc tctcccgaaa ccggcagaac tttgcgcagg agctgggaaa aaagatggaa
     9361 caagagctgg cagatctgca catggcgggc gccaagttca aagctgactt gcagcacgaa
     9421 cccgacgaga acggcttgct gatggatggg caatccaggg tgcgtttcga tgagaacggc
     9481 atcgaccagg tggaattcct gatcgccccc aaccccgggg aagggttgaa accgctggtc
     9541 aagatcgctt cgggcggtga aacttcccgt ttgatgctgg cgttgaagaa cgtgctggct
     9601 gaagccgata ccatccccac cctcatcttt gatgagatcg accagggcat tggcggaagg
     9661 atcggcgcga tcgtgggaga aaaactgtgg cagttgggca aaaatcacca agtgttgtgc
     9721 gtgacccatc tgccgcaact ggctgcctat ggcatccagc attatcacgt ttacaaacac
     9781 atccaagatg gcaggaccat caccatggtg gatgacatcc ggggagaacg gcgcattcat
     9841 gaactggctg cgatgttcgg tggtgacaca cccgccaatt gcgacgctgc gcaggaagcc
     9901 ctgcagagcg ccagccagcg aaccaccgca ttatcgggtg cacaaaagta agagcaattt
     9961 cacaaaattg cttgacaatt ttcgtaaaac tgtgatataa aagaatcacc aaacgctagg
    10021 gtgcccccct caccctagcg tttggctttt aaacagccga atcatagatt ccagctataa
    10081 tggagtaaga atttctacaa cgctggagga attatgaaaa aaagatttaa attcttgcgc
    10141 acgctggcat ctgttttcaa gatcctaggc atctttcttg ccgctatctc cctggtatgc
    10201 gggatcattt tggtcgttct tgctctcagc aatgggaatt tctggagctt gttcggtttg
    10261 gatgccgcca ccggtttcac taccggcata gcttccggga ttctcaccct ggtgggagga
    10321 ttgctgtacg gattggtggt atatgggttc ggcgaaatga tctatgtgtt catctcgatt
    10381 gaggaaaaca cctacagaac cagcaccttt cttgaagaga tgcaaaaaga agagcagtga
    10441 tagcttgcac cccttaaaat gcagccacct tgacgattca agacggctgg ggtatagtat
    10501 gcgcactgaa ttctcaatgc ccccgtagcg caattggaca gagcgtttgc ttgcggagca
    10561 aaaggttgca gattcgaatt ctgccggggg caccaccttg gagcggatac accgctccgc
    10621 tcattttaat aactgcaagg ttcacatgaa tcactcaaaa aaacaaattc tcctcaccaa
    10681 tgacgatggc attgattcgc ccgggttgtg ggcggcggcg gcggcacttt ccaccattgg
    10741 gttcgtcacc gtggtcgcac cgcgcgaaaa atgctctgcc atggggcgcg gtttcggcag
    10801 gcatgaagac cacaagatcg aagtgaggaa aatgaacgtc aatgatcagg aatgggacgt
    10861 gcatgcggtg ggtggaaccc cttctgaaac ggtactgcac ggaattctgg aagtaatgcc
    10921 aaaaaaaccc gacttggtgg tttccggtat taattacgga gaaaacgtca gcaccgatat
    10981 aacctattcc ggaacagtag gcgccgccat cgaagccgcc tcgctaggcg tacctgcact
    11041 tgcaacttcc tatcaggtga acgaagggga ttggaattca tacgcacagg tagatttttc
    11101 ggtagccgca catttcacca ggttcttcgc agagcgcctt ctttctacaa aattgccacc
    11161 cgatgtccac ctgctcaatc tggtcattcc gcaaagcgcc actccttcca ccccctggca
    11221 ggtcacccgc ctggctcccg gtcgttactt caatccttat atcaatcgac agggcggatg
    11281 ggacgacgaa ggtcatatcc atgcgcgcat catcattcac gacgatcttc ccgaagattg
    11341 cgatgtggag gtagcgatca agaaaaaaat ggtgtcggtc acaccggtca gtttagattt
    11401 gacctcacgg gttgacttta aggctttgga ttcgttgttt cgaatctgat ggtctttttt
    11461 gcagcagatc catcaaagtt ctgatgaacc tagatgttga agcgaatttc gacaatatcg
    11521 ccatcctgaa tcacatagtt tttgccttcc acgcgcaact tgccgcggtt gcgtaattct
    11581 gcaacgctgc ctgcaaccat caggtcgtca tacgccatga tctcggcgcg gatgaagcct
    11641 tgctgcatat cgctgtggat ggtgccggct gcttcagggg cggtggcgcc tttgcggacg
    11701 gtccaggcgt gcacttcttt ttctccagcg gtaaagaatg agagcaatcc caacaattca
    11761 tacgattcgc ggatcatgcg gttgagccct ggttcttcga tgccatactc ctgcagaaaa
    11821 agctgggctt cctcttctgg taattgcgca atctccatct cgatctttcc ttgcagcgga
    11881 atcaatttgt acgcagcagc cgatgcggtt aatgggggaa ccggctgccc ttccgccagg
    11941 ttcaatacaa tcagcatggg tttgcgcgtt aaaaaaccga atccctggat catgcgctct
    12001 tcttcatcgt caagcaccag ggtacgcagc ggtttttctt gctgcaagct ttcgttgagc
    12061 ttctcaaata gcctggtttc gcgttcgact tcgcctttgt cgcgcccgcc cttgtgccca
    12121 tcttcgtgca ggcgcgccag tttggcttcg attttgatct ggtcgttgag caacagttcc
    12181 gcatccatac tcgcgatgtc acgcgctgga tcaatactgc tcagcacatg agggatattt
    12241 tcatcgtcaa aacagcgcac cacctggatg aaaccatcca tttgcgatag ctggttgagc
    12301 agagaaccgg aaagttcgcc tttgttcccg cttcccaagc cggcgatgtc cacataggta
    12361 accttggcgc gcacggtctt gcgagcggaa aagaattcca ccagtttatc cacgcgaata
    12421 tccggcacat ctaccacacc ggtatggatc tccatctttc ccgctgacat atcggtgggt
    12481 tgggaggcac gggtaagagc gttgaagatg gtagtcttgc cagattgcgg cagtccaatg
    12541 atccctaatt tcatagaaaa atcctcatca acgatttctt ttatattgca cattccattt
    12601 taacccgaaa cagggaaact cgcctttgcc ttgaccggac tttcaaattt ggatatactt
    12661 tcttcatgcc tgccggagtg gcggaattgg cagacgcgca cgactcaaaa tcgtgttccc
    12721 tcgggattgt gggttcgatt cccaccttcg gcatcacaaa accagctttt tgctggtttt
    12781 tgttttaccc acaggctttt cttttcttgc tttccacgtc cctctcatcc tgttctgctc
    12841 gtataattgg gatagttcaa ggtcttaaaa atttgcacga tacaatcaaa tcactatctt
    12901 tctggagggt gctttgaata aacagttcaa gattgcgatc cccatggctg gtttgggaac
    12961 cagaatgcgc ccgcacacct ggagcaaacc caaaccgctc atccatgtcg ccgaaaaaac
    13021 ggttctcgat tacgtccttg accaattcag cagcgttccg gatcgaaaga acgccgaata
    13081 cgtcctgatc atcggaccca accagcgcga acagatcgaa ccctatatcc atgaaaagca
    13141 tcctgaaaag aaagtgcatt ttgtggtgca agagcagatg cgcggtcaat cagacgcgct
    13201 gtacctggca cgggaatatt tgcacggtgc ggttctcatg gctttttccg acacgctcat
    13261 cgaaaccgac ctttccttcc tggcagatga aaagatggat ggcattgcct gggtaaagcc
    13321 catccccgac ccgcgtcggt ttggcgtggc agaggtgaac gaaaccgggc gggttgcgcg
    13381 gctgatcgag aaaccgcgtg ataggaaaaa caacctggcg gtggtcggtt tttattattt
    13441 caaagaaggg cgagagctgg tcagcgctat tcaagaacag tttaaacgcg atatcagtct
    13501 aaagggagaa tactttctgg tagatgccat caacatcatg atcgagaaag gggcagcttt
    13561 taagacgaac agcgtggagg tctggttgga cgcgggcaaa ccagatgccc ttctcgagac
    13621 gaataaatat ctgctcgatc acggcagcgc caacagcgag caggcactga gccgtaaagg
    13681 gataacgatc gttccgccag tgtacatcca tcccagcgca acgatcagct cttgtgtgat
    13741 cggtccatac gcgtctatct cctctgcttg cgagatctcc aactccatca tccgcaattc
    13801 cattgtggaa gaaggggcgg tgatcaaaga gatgattctt gagaattctt tgatcggacg
    13861 caatgcatcc attgaagggc gcgccgaaca gatgaacatc ggggatgact cctggatcga
    13921 gatctagatt tccattttct gatttaaaac aaaaaaggga tgatttttct atctggattt
    13981 tctcaccatc ctcacgtata ctacgatgaa atttttccca cacatatcca gacaacaagg
    14041 agcagcaatg caaaaagatt acctgtgccg aaaagtgagg ggattacacc catccatcat
    14101 ccgacaattc tttgacattg ctgctaccat gaaagatatt atttcgctgg gtattggcga
    14161 acccgatttc accacccccg aacccatcgt caacgccggc atggaagctc tgcgccaggg
    14221 gcaaacccat tacacgtcca acatcggtat ggtcaaatta cgcgaagcga tcggtgcgca
    14281 tctgcagcaa ctgtacggcg taacctacca gcctgacgag atcatcatca cggtgggggg
    14341 ctcagaagct ttatatctgg cgaccactgc gttgctcgaa aacgaggatg aggtgatcat
    14401 ccccacacct tgctttgttt cctaccagcc cgaagtaacg ctggctggcg gcatcccggt
    14461 ggaagtgccc acctgtatgg aagagggttt caacctgaac atcccggcta tcgaaaaagc
    14521 catcaccccg cgcaccagag ctgtgatcgt caactctccc aacaac
//
LOCUS       DAOSDS010000053        13274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1814_length_13274_cov_4.823058, whole genome shotgun sequence.
ACCESSION   DAOSDS010000053 DAOSDS010000000
VERSION     DAOSDS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13274
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1814_length_13274_cov_4.823058"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PLL88_07330"
     CDS             complement(<1..614)
                     /locus_tag="PLL88_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HOJ01415.1"
                     /translation="MTTPIVAIVGRPNVGKSTLFNRLTGEPLAIVDDIAGTTRDRLFS
                     ESIWNGVHFQIIDTGGIDPSEGGKKPLSIASAEYIAQIRDQAMLAVEDADAVIFVTDA
                     AAGITPADQEVADLLRKHQKNRDGVRFPPIHLAVNKAENETLRISAMEFYALGLGTPI
                     PISALHGTGTGDLLDAVVASFPPEEVQEEDDSIKIAIVGKPNVG"
     gene            complement(617..1867)
                     /locus_tag="PLL88_07335"
     CDS             complement(617..1867)
                     /locus_tag="PLL88_07335"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOJ01416.1"
                     /translation="MMQLLKKLAKDMPTFLTALALAITIWILAVNANDPVEKRTYPVG
                     IPVEVVGLDSDLVITNEIPAEVTVSLSAPFSLWQTLIDSPQLVHARIDLEGLEEGTYE
                     ENVLVEVDAKPVKVETVGPASVHVIIEPLYSKYLPIQLVQPSQPAVGYEVGEPVLSHS
                     YATVSGPASLIAEVSEIRAILDVSQANQNIDKNITLAAFDENGIAVERVTIDPVQIHV
                     SLPITQRGGYRNVIVIPVVTGQPASGYQLTNKSVSPLTVTLYSADPSIVNNLPGYVET
                     QPLNINGASADITESLPLNLPAGVSIVGESTIRISITVSPIQGTITLNNSVIELVGLN
                     PEYSVNISPEVVEVIISGPLPVLDGLNASDVRVILDLTDLQPGIYQLNPRVEVNISDL
                     LIESIQPETIEVIITIPITATSEN"
     gene            complement(1864..2754)
                     /gene="cdaA"
                     /locus_tag="PLL88_07340"
     CDS             complement(1864..2754)
                     /gene="cdaA"
                     /locus_tag="PLL88_07340"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOJ01417.1"
                     /translation="MKDIISEIVFLFQRFSWLTIFDLLIVSLIFYAILRLLHNTQART
                     LFRGMIFVVIIIMLLTTLVDLPAFSYLVQNTLPAMFLAIPVIFAPEIRRALERVGRAG
                     PEKLFARKTTLPQEQIKNTISAVAVAVKRLSSLQHGALIVFQVADHLQEYIETGVAMS
                     SKVSAELILQIFYPNTPLHDGAVIITADTISAASCVLPLSSMGILNKTPDNQMGLRHR
                     AALGITESTDAVAVVVSEETGDISIAHLGKMIREVKSENLETALFPFLLEEERLVQST
                     NLEDLLRTWLGLEKKERKKE"
     gene            complement(2777..3886)
                     /gene="coaE"
                     /locus_tag="PLL88_07345"
     CDS             complement(2777..3886)
                     /gene="coaE"
                     /locus_tag="PLL88_07345"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOJ01418.1"
                     /translation="MKTPFVIGISGSIGSGKSLVRHLLALRGVLTIDADELTHLLMLK
                     GKAGYMAVLQIFGDRILTSDGAVERGKLGQIVFHDPQELQKLEEAIHPLVAKTVRAIL
                     QQTTCPLVAIEAIKLYSSELLSFVNSRWFITASREVQFDRLAQTRGMSREMILERVNQ
                     QPFPQRLQIDHVIENNQQIEDSWERVEQVWLKMLENQAAFRSAVNKLAEKIPAVFLDI
                     PQIARMESDKYWFDKSFMPVENGDVLQQHLLQNRIFLTPLHEDDAYLFWRFDHFNVII
                     EDISHPSSEKAIIAGISAIEAIAACWGANCVIVQTSKERAHCEVLSGQGYRRVSLKDK
                     GAFPFLQFGVSFEGTMETLFIKQLPAGIWRLIPER"
     gene            4013..4085
                     /locus_tag="PLL88_07350"
     tRNA            4013..4085
                     /locus_tag="PLL88_07350"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4046..4048,aa:Phe,seq:gaa)
     gene            4152..5348
                     /locus_tag="PLL88_07355"
     CDS             4152..5348
                     /locus_tag="PLL88_07355"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HOJ01419.1"
                     /translation="MRSRTFKVGLCLFLTVFLITNLTACSAPLAYLTFSDDSTIHQIS
                     IRGKTLSEILDNAGYPLGENDRTLVNGVTTDTTAEISLKNGDTIQVQRRYPITIVDDG
                     AKHTILSSAHTLAQALWENQIFVSASDFISIPLDTIIDQSLSLEIQRSNTLTIQADGQ
                     EITSSASAQTVGDALAQAGISLQNLDYSIPAEDDPLPSDGIIRVVRVSEEIQIIEEIL
                     PYETEYVADAEMNLDELKVIQAGQSGVSMSRVHIRTENGEEVARETEESWIAVAPLAQ
                     QTAYGTKITIQTASTENGVIEYWRAVTVTANSYLDTGSKTTSGKWPTYGMVAVSSSWW
                     SYMKGTSVYVPGYGVATVEDKCGACEGKMLIDVFIPTADYVPWHKTVTLYFLTPIPAS
                     IKYVLP"
     gene            5356..6195
                     /gene="rsmA"
                     /locus_tag="PLL88_07360"
     CDS             5356..6195
                     /gene="rsmA"
                     /locus_tag="PLL88_07360"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOJ01420.1"
                     /translation="MLYQNPIALLKEHHLAPKKKLGQNFLVDGNVLNRIVALADVNEN
                     STVLEIGAGLGSLTFFLAQAAKHVIAVEVDEELLPIARQLLQGCSNVTLVAGDILALD
                     LNSLRLPNGYTVVANIPYYITSAIIRKLLTATEKPKHMLLTVQEEVAERICAQNGQMS
                     LLALSVQVFGKPEIKMTIPAGAFHPAPAVDSAVLRIDLFQEPLIATEKLEAFFTLIKA
                     GFSQKRKMLRNTLSAGLHLPHEQVEEFLIRAGVNPQRRAQTLSMEEWQSATDNFLLQS
                     AKG"
     gene            complement(6197..6925)
                     /locus_tag="PLL88_07365"
     CDS             complement(6197..6925)
                     /locus_tag="PLL88_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOJ01421.1"
                     /translation="MKKTTIVIPTFNERENLPKLFAEMFALPVNDLHILVVDDNSPDG
                     TGQLVEELKKSYQGKLDILHRAGKLGLGTAYIQGFQQAMQEGAQVIGQMDADFSHPIP
                     KIPEMLAALDEVDLAIGSRYIPGGKVDETWSFWRKGLSRFGNFYARTILFLPMNDVTG
                     GFRFWKAQALQRLPLERVRSSGYVFQVEMAFLAYRLGLTFREVPIYFADRRWGTSKMS
                     FRIQLEAAYRVWLLKSMYSDLWEK"
     gene            7005..8093
                     /locus_tag="PLL88_07370"
     CDS             7005..8093
                     /locus_tag="PLL88_07370"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOJ01422.1"
                     /translation="MDIKKVPAIIIVIFSVMLAACNIRISTITDEDKVNTAVAQTIVV
                     QQQATVTPLILPTITGLPTITQQSNEPTGTPQPCNKAAAVSESYPDDSEVTIGTDFDK
                     SWRLQNIGTCTWNSNYRIVFYDGDQMGGPNSQKLNTTVAPGEVTDIIIDQKAPNAAGT
                     YKGTWKLQDDSGANFATVWVQIKAVNQVVQIQPPLHLLFNIKTVSQASGEGGSVRSDG
                     TVMSGLYNVGDLPTNGSSQVFVSFDISGIPADATITEVRVDFTDFDMLDDPFGDLGCL
                     RAYVQNYGNLDAGDYFGGVPIGAIGRWCSAGELQTVAVDDDFKSALQSAVGNSRFRIR
                     LQFSDKLSDNDNTGDMVRFGTIKLRVIY"
     gene            8186..9091
                     /locus_tag="PLL88_07375"
     CDS             8186..9091
                     /locus_tag="PLL88_07375"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOJ01423.1"
                     /translation="MKRKIKALFLFAVLIGLLAGCNFPLASSRSGEDAVATSVAQTVA
                     AVDAQNQPAATVVILPTNTLPSLPTITPQATSTSISTIPTSTPQPCNQAWLASESPLD
                     DAVYSPGAAFTKTWNLKNIGTCTWNTSYKAVFISGSSMGGPASKALTANVAPGETLAL
                     SLSLTAPTTAGTYKGIWNLQDDTGKNFANFWVQIKVQSAPFAVTSVSLSAEDADITGS
                     CPQTFNYEAVVTTNGAGTVTYYFTYSDGTTGATKSMVYNAAGSKTLSGSWQLSADGSY
                     WIKLYIDNPNHQLFGPLNLTLDCTP"
     gene            9261..10487
                     /locus_tag="PLL88_07380"
     CDS             9261..10487
                     /locus_tag="PLL88_07380"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HOJ01424.1"
                     /translation="MKKAFTILALTIIFATLISACGSRSARNAEVATAIAQTVEAMNA
                     QAPLVTYVVVTPDSTSTPLPTNTAVPIPTATSAPTSVPRSCNAISFISENPLDGVTYA
                     PGETFTKSWRFKNVGTCTWNPHYKVVYASGNSISGAGTKQLNTYVVPGETIDIVYTFK
                     APSTAGTYKTVFYLKDDEGNTFAQFWVQFKVKSTSATSVPTPGFAVTSVTFYSSDAVK
                     TGTCPQTFDYNAVITANKAGTVTYHWEFSDGSAGSTQSVTFNAAGSKTVSGTWNLNAS
                     GAYWVKLYIDNPNHQLFGQLNLSLTCTPAFAVTGVTFPAPTTPYTASTCSPLALDYQI
                     DVAANGAGTVTYSITFSDSTSTNGSLPFSSAGTQSITGTWNVGTATSGTYWFNIYIDN
                     PNHQLFGPHTIVVTCP"
     gene            10712..11479
                     /locus_tag="PLL88_07385"
     CDS             10712..11479
                     /locus_tag="PLL88_07385"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HOJ01425.1"
                     /translation="MFTFWLLIALLALVLFVLLPMGIRMLYQYERGVVFTLGKFSNVR
                     NPGLTFLVPFIQTMRKVDMRLKTAEVPRQEVITKDNIPILVNAVVYFKVINPEKVIIE
                     IEDYIFAIRQYTQAALRDVIGNSDLDFVLTEREKVAGSIKEIVDSETSNWGVDVESIK
                     IQEIELPAEMKRAMAVQAEAERNRRAVIIASQGELSASENLRQAAENLAKSSGALHLR
                     TLQTLRDIAADPSEKIVMFMPWEIGEAVTQMVSKRKE"
     gene            11476..12150
                     /locus_tag="PLL88_07390"
     CDS             11476..12150
                     /locus_tag="PLL88_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01426.1"
                     /translation="MIKHLDNKTRYLQIAFNYDARMAQYILPQIPQHPRILIEAGTPY
                     IKREGIRGIMLIRRMWSGLVVADIKVVDGALGEVEMVHRAGADAITVLGNAPRETLDL
                     VIATCQKLNMISMVDMVGVHDPLKVIMALKKPPEVVVLHRGRDEESTRNKVIQYRHVN
                     RLRSKYSMLISAAGGLDVKGSRSAIFNGANIVVANLVQPGDRWTGISTQENVGEMAQA
                     FLTTIE"
     gene            12230..13210
                     /locus_tag="PLL88_07395"
     CDS             12230..13210
                     /locus_tag="PLL88_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01427.1"
                     /translation="MAENKKKFLFPTLTLFLTAPIFGEVLSTSSPPTEFFQPATFLIL
                     VGLYGSGALLVRECVRRWQKGWISVLLLGMAYGIYEEGLVVRSFFDPNWVDIGVLGSY
                     GRMGGINWIWSIALTIFHAVVSIGLPILVVELLFPQHRAESWLGKTGLVITGVVFASF
                     FFFAPLLKLYAIPAGKFLLCLACMAGLILLAYHWKDGWLAVKQKKTAKPGKIGLSGFV
                     WMLLFALEMWLLPALNLPAWLDFCLLAALPWLGVWQMKRWDCTEWSERQQWALAMGLL
                     TPWFITASIAELQNAARADDTRGLIAASLLVLASMLLLRLQIGKREKERE"
BASE COUNT         3418 a         3527 c         3308 g         3021 t
ORIGIN      
        1 ttcccgacat tgggtttccc gacaatggcg atcttgattg aatcatcctc ttcctgtact
       61 tcttccgggg ggaaggaagc caccacggca tccagcagat cccctgtgcc ggttccatgc
      121 aaagcagaaa tggggatggg cgttccaagc cccaatgcat agaattccat ggctgaaatg
      181 cgcaaagttt cattttcggc tttattgaca gccaggtgaa tggggggaaa tcgaacgccg
      241 tcacgatttt tttgatgctt gcgcaacaga tcagccactt cttggtctgc tggggtaata
      301 cccgcggcag cgtcggttac gaatatgacc gcatccgcat cttccacagc gagcatcgcc
      361 tgatcacgga tctgggctat atattccgct gatgcgatgc tgagcggttt ttttcccccc
      421 tcgctgggat caatgccccc ggtatcgatg atctgaaaat gcacgccgtt ccagatcgat
      481 tcagaaaaaa gccggtcacg ggtagttcca gcgatatcat ccacaatagc caggggttcc
      541 cctgtcagac gattaaaaag ggtgctcttc cccacattgg gtctgccaac aatagcaacg
      601 atcggtgtgg tcatgattag ttttcagacg tcgcggtgat gggaatggtg atgatcacct
      661 cgatcgtttc cggttggatc gattcaatca aaagatcgga gatattcacc tcaacgcgag
      721 ggttcaattg gtagatgccc ggttgaaggt ccgttaaatc aaggatcact cgcacatcgc
      781 tcgcattcaa accatccaaa acaggcaatg gtcctgagat aatcacttca acaacttcag
      841 gggaaatatt caccgagtat tcagggttca gtcccaccaa ctcgatcacc gaattgttca
      901 aggtgatcgt cccctggatt ggggaaactg tgatcgatat tctgatggtt gattcgccaa
      961 caatacttac ccctgcaggc agattcaaag gaagagattc tgtgatatca gcactcgcac
     1021 cgttgatgtt caaaggttgg gtctcaacat aaccgggcaa attattcaca atagaaggat
     1081 cggctgaata caatgttaca gtgagcggtg agaccgattt gttggtcaat tgatagccgc
     1141 tggctggttg accggttacc acaggtatca caatgacgtt acgataccca ccacgctgcg
     1201 taatcggcag ggatacgtgg atctgcaccg gatcaatggt gacacgttcc accgcaattc
     1261 cgttttcatc gaaagcagcc agtgtgatat ttttgtcgat gttctgattt gcctgactta
     1321 catccaggat agctctgatc tcggacacct cggcaatcag tgaagccggt ccactgacgg
     1381 tggcatagct atgactcaga accggttctc ccacttcata ccccaccgct ggttgagaag
     1441 gctgcaccaa ttggatgggc aggtatttgg aataaagggg ttcgatgatc acatgcacac
     1501 tggcaggacc aaccgtttca accttaaccg gttttgcatc cacttctacc aagacgttct
     1561 cttcatacgt tccctcttcc aacccctcca aatctatcct ggcatggacc aattgagggc
     1621 tgtcaatcag cgtctgccac aaggaaaagg gagcactcaa actgacggtc acttctgcag
     1681 ggatttcatt ggtgatcacc aggtcggaat ccagccccac cacttccacc ggtatcccaa
     1741 ccggatatgt gcgtttttca accggatcat tggcattcac tgccaggatc cagatggtaa
     1801 tagcgagtgc caatgcggtc aggaaggtag gcatatcttt tgccagcttc ttgagtagct
     1861 gcatcattct ttcttccttt cctttttttc aagccccagc caggtgcgta aaaggtcctc
     1921 aagattggtg gactgcacca gccgctcttc ctctaacaaa aaaggaaaca gtgcggtttc
     1981 aaggttttct gattttacct cacggatcat ttttccaaga tgagcgatgg aaatatcacc
     2041 cgtctcttca gaaaccacca ccgctaccgc atcggtagac tcggtgattc ccaaggctgc
     2101 ccggtgacgc agccccattt ggttatcagg cgttttattc aagatgccca tcgaagacag
     2161 cggcagcacg caggaagcag cgctgatggt gtcggcagtg atgatcaccg ctccatcatg
     2221 caagggcgta ttggggtaaa agatctgcaa aatcaattcc gctgaaacct tggaactcat
     2281 ggcaacgccc gtttcgatat actcctgcaa atgatcggca acctgaaaaa cgatcaatgc
     2341 gccatgttgc aacgaagata accgttttac tgcaactgcc acggcgctga tcgtgttttt
     2401 gatctgttcc tgtggcaggg ttgtcttacg agcaaacagc ttttctggtc cagcgcgccc
     2461 caccctctcc aacgcccgcc gtatttcagg agcaaaaata accgggatgg ctaaaaacat
     2521 tgccggcaga gtgttctgca ccagatagga aaacgccggc aggtcgacca gcgtggtcag
     2581 cagcatgata atgatgacca caaagatcat gccgcgaaac agcgtgcgag cctgggtatt
     2641 gtgaagcaag cgcaatatcg cataaaagat caagctaacg atcaacagat cgaagatcgt
     2701 caaccaacta aagcgttgaa agaggaatac gatttctgaa ataatgtctt tcatcttttt
     2761 aagtttacat caatccctat ctttcgggaa tcagcctcca aattccggcg ggcaattgtt
     2821 tgatgaaaag ggtttccatt gtaccttcaa atgacacccc aaactggaga aatgggaagg
     2881 cacctttatc tttcaaagat acccttcgat aaccctgacc agaaagtact tcacaatgag
     2941 cacgttcttt tgatgtctga acgatcacgc agttggcacc ccaacaggca gcgatcgctt
     3001 cgatcgccga aatgcctgca atgatcgctt tttcactgct ggggtgcgaa atatcttcaa
     3061 tgattacgtt gaaatggtca aacctccaga aaagataagc atcatcctca tgcaaaggtg
     3121 taaggaaaat gcgattctgc aacaggtgct gctgtaagac atcaccattt tcaaccggca
     3181 tgaaggattt gtcaaaccaa tacttgtcac tttccatcct ggctatttgc ggaatatcca
     3241 gaaacacagc cgggattttc tccgccagtt tgttcaccgc cgaccgaaag gcagcctgat
     3301 tttcaagcat ttttaaccac acttgctcca cccgttccca ggaatcttct atctgctggt
     3361 tattttcaat gacgtgatcg atttgcagcc tctgcggaaa gggctgctga ttcacacgct
     3421 ccaagatcat ctctcgactc attccgcgag tttgcgcaag acgatcgaat tgaacctctc
     3481 tactggcagt gatgaaccag cgcgaattca cgaaagacaa cagctcagaa gaatacagct
     3541 taatggcttc aatggcaacc agcgggcagg ttgtctgttg taaaatggcg cgcacagtct
     3601 tcgccacaag cggatgaatc gcctcttcca gcttctgcaa ttcctgtggg tcatggaaga
     3661 cgatctgtcc caacttaccg cgctccactg caccatcgct cgtcaagatg cgatcaccaa
     3721 aaatctggag cactgccata tagccggctt tccctttgag catcagcaaa tgggtgagct
     3781 catctgcatc gatggtcaat acaccgcgca aagccagcag atgccgcacc agactcttgc
     3841 cgctgccgat acttccagag atgccgatca caaaaggtgt tttcacgttg atttatcaat
     3901 ccttgctcac cttcatttta atagacacgc agtttctgtc aaacattcca tcaaatattc
     3961 ctgatttgct tgacaatgcc ttcacaagca tgtagaattc catcacgatt tcgccgagat
     4021 agctcagttg gtagagcacg cgactgaaaa tcgcggtgtc gccagttcga ttctggctct
     4081 cggcatcaaa accccgcaaa acggggtttt atttttaagt tacaatgttt tccatgttct
     4141 accagcttaa tttgagatca cgcactttca aagtgggtct gtgcttattt ttaacggtct
     4201 tcttgatcac caatctgacc gcctgcagcg ccccgctggc ctacctgact ttcagtgacg
     4261 attccaccat tcaccagata tccatacggg gaaaaaccct gagcgaaatt ttggacaatg
     4321 ctggctatcc actaggagaa aatgatcgga cgttggtcaa tggggtcacc accgatacta
     4381 cagcagaaat cagtctcaaa aatggtgaca cgattcaggt gcagcgcaga tacccaatca
     4441 ccatcgtaga tgacggggcg aagcacacca ttctcagcag tgcgcacacc ctcgcccagg
     4501 cgctgtggga aaatcagatc ttcgtttcag ccagcgactt catttccatc ccccttgata
     4561 cgatcatcga tcaatccctt tcccttgaga tccaacgcag caacacgcta acaattcagg
     4621 cggacgggca ggagatcacc agcagcgcat ccgctcaaac cgttggtgac gcgctggcac
     4681 aagccggaat ttccctgcag aatctggatt acagcatccc tgcagaagac gatccgctgc
     4741 cctcagatgg catcatccga gtagtacgcg tttccgaaga gatccaaatc atcgaagaga
     4801 tcctccctta tgaaaccgaa tatgtggcgg atgcggaaat gaacctggat gaactgaagg
     4861 tcattcaagc cgggcagagt ggtgtcagca tgtcgcgggt tcacatccgc acagaaaatg
     4921 gcgaggaggt ggctcgcgaa accgaagagt catggatcgc tgtagccccg cttgcccagc
     4981 aaaccgcata tggaaccaag atcaccattc aaaccgccag cactgaaaac ggggtgatcg
     5041 agtactggcg cgcggtaact gttacagcaa actcttacct tgacacgggt tccaaaacca
     5101 ccagcggtaa atggccaacc tacggaatgg ttgcagtttc cagctcatgg tggtcataca
     5161 tgaaaggcac gtctgtatat gttccgggat atggcgtcgc gactgtggag gataaatgcg
     5221 gcgcctgcga gggaaaaatg ctgatcgacg ttttcatccc tacggctgat tatgttccct
     5281 ggcataagac ggttaccctg tatttcctca cccccatacc ggcatcaatc aagtatgtcc
     5341 ttccataaat aaagaatgct ctatcaaaac ccaatcgcgc tcctgaaaga acatcacctg
     5401 gctcccaaga agaagctggg acaaaatttt cttgtggatg gcaatgtcct caaccgcatc
     5461 gttgcgctgg cagacgttaa tgagaattcc accgtgctgg agataggggc tggattgggc
     5521 agcttgacct tctttctggc acaggctgcc aaacatgtta ttgccgtaga ggtggatgaa
     5581 gagctgctgc ccatcgccag acagttgttg caagggtgtt ccaacgtcac cctggtggcg
     5641 ggggatattc tggcactcga tctgaatagc ttgcgtctcc caaatggtta caccgtggta
     5701 gccaacatcc cctactatat cacctccgcc atcatccgca aattgctaac agccacagaa
     5761 aaaccaaagc acatgctatt gacggttcag gaagaggtgg ctgaacgcat ttgcgcacag
     5821 aatggtcaaa tgagcttgct ggcgctcagc gtacaggttt tcggaaagcc cgaaataaaa
     5881 atgactatcc ccgctggtgc atttcatcct gcgccggctg tggattcggc agttttgcgc
     5941 atcgatttgt ttcaggaacc cctcatcgcg acagagaagc tggaggcatt cttcacgctg
     6001 atcaaagccg gtttcagcca gaagcgcaag atgctgcgca acaccctttc ggcagggctg
     6061 cacctcccgc acgaacaggt agaagaattc ctcatccggg caggcgtcaa ccctcaacgc
     6121 cgcgcacaga ccctttctat ggaagagtgg caatcagcga cggataactt tcttttgcaa
     6181 agcgcaaaag gatagcttac ttctcccaca aatcggaata catgctcttc aatagccata
     6241 cgcgataggc tgcttctaat tggatgcgga acgacatttt ggaggtgccc cagcgacggt
     6301 cagcaaaata gatggggacc tctcgaaagg tcagccctaa cctgtatgcc aggaaagcca
     6361 tttctacctg gaaaacatag ccgctcgaac gcaccctttc cagcggcaaa cgctgcaatg
     6421 cctgggcttt ccagaagcga aaaccgccag tgacatcgtt cataggcaag aaaagaatgg
     6481 tgcgggcata aaaattcccg aacctggaga gccctttccg ccagaacgac caggtttcat
     6541 ctacttttcc tccggggatg tagcgcgaac cgatggcgag atctacctcg tccagcgccg
     6601 ccagcatttc ggggattttt gggatgggat gggaaaaatc cgcatccatt tgcccgatca
     6661 cctgcgcacc ttcttgcata gcctgctgaa aaccctgaat atacgctgtc cccagaccaa
     6721 gctttccagc acgatgcagg atatccagtt taccctggta ggatttcttt aattcttcaa
     6781 ccaattgccc ggtgccatct gggctgttat catccacaac caggatatgc aaatcattca
     6841 ctggaagggc aaacatctcc gcgaacaatt ttggtaaatt ttcgcgttca ttaaaggttg
     6901 gaataacaat ggttgttttt ttcaatgaaa acctctacca tgggatgatg acataatctt
     6961 accttaatat agtaacacct gcttacagga agaaggagag aaaaatggat atcaaaaaag
     7021 ttccggcaat aattatcgtt atattttccg tgatgctggc agcctgcaat atccgcatca
     7081 gcaccataac agatgaagac aaggtaaata cggcggtggc gcagacgatc gtcgtccagc
     7141 agcaagccac cgttaccccg ctgatattgc ccaccattac cggtctgcca accattacgc
     7201 agcaatccaa tgaaccgacg ggcaccccac aaccttgcaa taaggcggct gcggtgagtg
     7261 aaagctaccc ggatgatagc gaggtcacaa tagggacaga tttcgataaa tcctggcgtc
     7321 tgcagaacat cggcacctgc acctggaact ccaactaccg catcgtcttt tacgatggag
     7381 accaaatggg gggtccgaat tcgcaaaaac tgaacaccac cgtagctccc ggggaagtga
     7441 ccgacatcat catcgaccag aaagcgccca atgctgccgg tacctataaa ggaacgtgga
     7501 aattacagga cgacagcggc gcgaattttg ccaccgtgtg ggtgcagatc aaggcagtga
     7561 accaggtagt gcagatccag cctccattgc atctgctttt caatataaag actgtcagcc
     7621 aagccagcgg agaaggtggg tctgttcgtt cggatggaac cgtgatgtcg ggtctctata
     7681 atgttggaga cctgccgacc aacggcagct cccaggtatt tgtcagtttc gatatcagcg
     7741 gcattccggc agatgccacg attacagaag tgagagttga ctttaccgat tttgatatgc
     7801 tggacgatcc ttttggcgat ctgggctgcc tgcgcgcgta tgtccagaat tatgggaatt
     7861 tggatgccgg agattatttt ggcggcgtcc cgataggtgc aatcgggcgc tggtgctcgg
     7921 caggggaact gcaaaccgtt gctgtggatg acgatttcaa gagcgcgctg cagagtgcgg
     7981 taggaaattc ccgcttcagg atacgtttgc aattcagcga caagctcagc gacaacgaca
     8041 atacgggaga tatggttcgc tttggaacca tcaaactgcg tgtaatttac taacaatgac
     8101 cataaccctg atttgacata ctattcaccc gctatcgact ataatagcgg gtgaactctt
     8161 taataaagaa gtggaggagg tttccatgaa aaggaaaata aaagcattgt ttttgtttgc
     8221 cgtcctgatc ggacttttgg cggggtgcaa cttcccgctg gcgagtagca gatcaggcga
     8281 agacgccgtt gcaacctccg tcgcgcagac ggtggcggca gtggatgcgc aaaaccagcc
     8341 cgcagcaacg gtcgtgatcc tgccaaccaa taccctgccc tctctgccaa ccatcactcc
     8401 tcaagccaca agcacgtcca tctcaacgat acccaccagc acaccgcagc catgcaacca
     8461 ggcctggttg gcatctgaaa gcccgctgga tgatgcggtc tacagccccg gagccgcctt
     8521 caccaaaaca tggaatttga agaacattgg cacctgcacc tggaacacca gctacaaggc
     8581 agtgttcatc agtggctcat cgatgggcgg acccgccagc aaagcgctga cagccaatgt
     8641 ggcacccggc gaaaccctgg cgctctcact ttccctcacc gcacccacca ctgccgggac
     8701 atataaggga atatggaatt tgcaggatga cacgggaaag aactttgcca atttctgggt
     8761 gcaaatcaaa gtacaatcag ccccattcgc ggtcaccagc gtgagccttt cagcggaaga
     8821 cgcagatatt acaggcagtt gtcctcaaac tttcaactac gaagctgtgg tcacaaccaa
     8881 tggagctgga acggtgacct attatttcac ctacagcgat ggcaccaccg gcgcgacaaa
     8941 gtcaatggta tacaacgctg ctggatcaaa gacccttagc gggtcatggc aattgagcgc
     9001 cgacggcagc tattggataa agctgtacat tgacaatccc aaccaccagc tattcggacc
     9061 actcaatctg acattagact gcacgccata aaacccatcc tgccggtcct cataggcagt
     9121 gcgaaatgca ctgcctatgt ttccaggaca gcggtatgca acaaccagaa ttcgagcggc
     9181 gggaacaaaa tagaattcag gacgttaagt taatagaata agcgaaccag cgcttaatgc
     9241 gtttttcgcg gaggtcaaat atgaaaaaag cattcaccat attggcactg acgatcatct
     9301 tcgccacact catttcagct tgcggaagtc gcagtgctcg caatgcagag gtagcaaccg
     9361 ccatcgcgca aaccgtagaa gccatgaacg cgcaagctcc tctggtcacc tacgtggtgg
     9421 tcacgccaga cagcacttca acacccttgc caaccaacac agccgtccct atccctactg
     9481 ccacgtcagc gcctaccagt gttccgcgct cctgcaatgc catcagcttc atcagtgaga
     9541 acccactgga tggggtaacc tatgccccgg gtgaaacatt caccaagagc tggcgtttca
     9601 agaacgtggg cacttgcacc tggaacccgc actataaagt agtttatgcc agcggcaaca
     9661 gcatcagcgg agcgggtacc aaacagctca acacgtatgt cgtgcctgga gagacgatcg
     9721 atatcgtgta cacgtttaaa gcgcccagca ccgccggcac atacaaaacc gttttttatc
     9781 tcaaggatga cgaggggaac actttcgccc aattttgggt gcagtttaaa gtgaaatcca
     9841 cttccgctac cagcgtaccc actccgggat ttgcagtgac cagcgtaact ttttattcga
     9901 gtgacgccgt gaagaccggc acctgtccgc agacttttga ctacaacgcg gtgatcacag
     9961 ccaacaaagc aggaacggta acctaccact gggagttcag cgacggctct gccggctcaa
    10021 cccaatccgt caccttcaac gcggcaggat ccaagactgt ttccggcacc tggaatctga
    10081 acgccagcgg agcgtactgg gtcaaactat acattgacaa tcccaaccac cagttgttcg
    10141 gtcagcttaa cctgagcctg acctgcacac cggctttcgc cgtgaccggg gtgacttttc
    10201 cagctcctac aacaccttac acagcctcaa cctgttcgcc gctagcactt gattatcaaa
    10261 ttgatgtcgc cgcaaatggc gcaggaacgg tcacctactc gatcactttc agtgacagca
    10321 cctctacaaa tggttcactc ccattcagtt cggcaggaac gcaatctatc accggcacct
    10381 ggaatgttgg cacagccacc agtggcacct actggttcaa tatttatatc gacaatccta
    10441 accaccagtt gttcggaccg cataccattg tggtaacttg cccttagaac caagttgatt
    10501 gatcagcatg cttgacaata gctgacaaaa cctgacagcg gcacaataca acgtgattcg
    10561 ttcctgagga acgaatcacg ttttgttcga gaacgtatat atataatgag gaggagaccc
    10621 ataagagaag aggctcacac tgttaactat ccattaatac aattttcaat ttccatccat
    10681 acaatgtaag atagagaaga aaggagtttt tatgttcaca ttctggcttt tgatagcgtt
    10741 attggcattg gtattgtttg tgctgctccc tatgggcatc cgcatgctct atcagtatga
    10801 acggggcgtg gtgttcacat tgggcaagtt cagcaacgtg cgcaaccccg gattgacctt
    10861 cctggtgccc ttcatccaga ccatgcgcaa ggtggatatg cgcttgaaga ccgcagaagt
    10921 gccgcggcaa gaggtgatca ccaaagacaa catccccatt ctggtgaacg cggtggtgta
    10981 cttcaaggtg atcaacccgg aaaaagtgat catcgagatc gaagattaca tcttcgccat
    11041 ccggcagtac acgcaggcag ccctgcgcga tgtgatcggc aacagcgacc tggatttcgt
    11101 gctgaccgag cgcgaaaagg tggcgggcag catcaaggag atcgtggatt ctgagacgag
    11161 caattggggg gtggacgtgg aatccatcaa gattcaggag atcgaactgc ccgcggagat
    11221 gaaacgcgcc atggcagtgc aggcggaagc cgaacgcaac cggcgtgcgg tgatcatcgc
    11281 ctcgcaaggc gagctttcgg catcggagaa cctgcgtcag gcggctgaaa acctggcaaa
    11341 gagcagcggc gcgctgcacc tgcgcaccct gcagaccctg cgcgacatcg ctgccgaccc
    11401 ttcggagaag atcgtgatgt tcatgccgtg ggagatcggt gaagccgtga cgcagatggt
    11461 cagcaaacga aaagaatgat caagcatctc gataataaaa cgcgctacct gcagatcgcg
    11521 ttcaattatg acgcccgcat ggcgcagtac attctgccgc agattccgca acacccgcgc
    11581 attctcattg aagcggggac accctacatc aaacgggagg ggatcagggg gatcatgctc
    11641 atccggcgca tgtggagcgg gctggtggtg gcagacatca aggtggtaga cggcgcactg
    11701 ggcgaggtgg aaatggtgca ccgcgcggga gcggatgcca tcacagtgct gggaaacgcc
    11761 ccgcgcgaaa cgcttgacct ggtcatcgcc acctgccaga agctgaacat gatctcgatg
    11821 gtggatatgg tgggggtgca tgacccattg aaggtgatca tggcgctcaa gaaaccgccc
    11881 gaggtggtgg tgctgcaccg cggcagggat gaggaaagca cgcgtaacaa ggtcatccag
    11941 taccggcatg tgaaccggct gcgcagtaaa tatagcatgc tcatctcagc ggccggcgga
    12001 ctggacgtga agggctcgcg cagcgccatc ttcaacggcg ccaacatcgt agtagcgaac
    12061 ctggtgcagc ccggggaccg ctggacgggc atctccaccc aggagaacgt gggcgagatg
    12121 gcacaagcgt tcctgaccac catcgagtaa agcagcggtg aattcatagg ttcacacaga
    12181 tgatgccgat gatattctta cggcattatt tgtgtaatag atgaatggta tggcagagaa
    12241 taagaagaaa tttctctttc ccacgcttac cctgtttctg actgcgccga ttttcgggga
    12301 agtattatcc acctcctccc cgcccacaga atttttccag ccagccacct ttctgatcct
    12361 ggtcggactg tatggaagcg gcgcgctgct tgtgcgggaa tgcgtgcgca ggtggcagaa
    12421 aggttggata tccgttctgc tgctgggaat ggcatacggc atctatgaag aaggactggt
    12481 ggtgcgtagc ttcttcgatc caaactgggt ggatatcggt gtcttgggca gctatggcag
    12541 aatgggcggt atcaactgga tctggagcat cgccctgacc atctttcacg cagtggtgag
    12601 cattggatta cccatcctgg tggtggaact gctgttcccg caacaccgcg cagaatcctg
    12661 gcttggaaaa acgggattgg tgatcacggg agtggttttt gcatctttct ttttcttcgc
    12721 ccctctacta aagctatacg caatccctgc cggtaaattt ctgctgtgcc tggcttgcat
    12781 ggcaggcttg atcttgctcg cgtaccattg gaaagatgga tggctcgcag tgaagcaaaa
    12841 gaaaactgcc aaaccgggca agatcggcct cagcggtttc gtctggatgc tgttgtttgc
    12901 gcttgaaatg tggttgctgc ctgccctgaa cctgcccgcc tggctggact tctgccttct
    12961 ggctgccctg ccctggctgg gagtatggca gatgaagcgc tgggactgca ctgaatggag
    13021 cgaacgccag caatgggcgt tggcaatggg actgctgaca ccctggttca tcacggcatc
    13081 catcgcggag ttgcagaatg ccgcccgggc agatgacacg cgcggtttga tcgctgcatc
    13141 cctgctggtg ctggcgagta tgctcctgct gcgcctgcag atcggaaaac gggagaaaga
    13201 gcgggaatag gactgttcat gttgggagaa gttgaaaagt gaacaccatt gaaattcaaa
    13261 agtgaacttt gata
//
LOCUS       DAOSDS010000054        12630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1925_length_12630_cov_5.188628, whole genome shotgun sequence.
ACCESSION   DAOSDS010000054 DAOSDS010000000
VERSION     DAOSDS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12630
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1925_length_12630_cov_5.188628"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLL88_07400"
     CDS             <1..487
                     /locus_tag="PLL88_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01428.1"
                     /translation="PITPFISTSYPEINRAITQKAWDHVQDLLRKITTISGAWTIGAS
                     LGLAIFGKWLLSLYGADFVPAYPTMMIFLIGLGFANIFFWNRPLLLSLKLPMVPYRAS
                     LYCGIAKVLLSFLLVPTLGLNTEAWLLSIFFLISISVIVLQGVKEIKKRAQPEQTGAS
                     L"
     gene            484..1653
                     /locus_tag="PLL88_07405"
     CDS             484..1653
                     /locus_tag="PLL88_07405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ01429.1"
                     /translation="MRIACITTSQIPSRTANSIQAMKVCHALKQCGQEIKLWVPFFKS
                     ADWTELKELYGLQEEFPIQWLPFKKSLRQYDFCWHAVQQARQWNADVIYTWALQSAVF
                     SQRQQIPSVMEFHDFPMGTFGPHLFKIYIRHHQRCLSLTTTAALAQGLEEKYGFTFPS
                     GSLQIAPNGTDPQCYLHLPSPVQARKKLGLPEGVTVGFTGHFYKGRGIELLLEIALAL
                     PDIHFLWVGGKEEHIQPWREELKARQINNVTLVGFIPNQELPLYQAACNILVMPYGKK
                     ISGSSGGDISQVINPMKMFDYMASGRAIVASDIPVFHEILSEQTAVFCPPEDSQGWSA
                     AIRRLADDQDERERLANNAKARSAQYSWTARAAKTLALINDLEGFRHEARLDQKS"
     gene            1625..3154
                     /locus_tag="PLL88_07410"
     CDS             1625..3154
                     /locus_tag="PLL88_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01430.1"
                     /translation="MKQGLTKNLSVSVPIAIGLGGLVCLVNPGEKPLVGWLGSAVLSF
                     VFLFGIVYAIQKLQSPKKVRLISLINFSVRVIAGIALFLMLPIFGYEEEPPQAGYLYL
                     DAYRRDANAWQIASDAEPIFTGFQDEFYTDQYGGMLSLSAVVYRAFSPDAHRPILILL
                     LTSFISTLGIPFYWQALQERFGNLTATIACWILALYPEAVILGGSQMREPLIIGLSGI
                     ILWGLEHRKINKRAGMIALLSAFLALMVISSRYAIAVSLLIAIWVWSDLTSQDTAKQK
                     KIWQILPLVVIILAGLLVSWEWLVDTSRWDLYLMESSSGRVQFELDNIGEQFRIPFLV
                     LYGILQPVLPAAIAYPGIAIMRVIAIFRSAGWYALAPTLLLAPFIMPKETNKRNRKIL
                     ILTTCFLFFWTLLSSLRAGGDQWDNPRYRTALLPWFALVAGWVFIYFKEHKNPWLKRA
                     YSILTVFCLFFLQWYFSRYYKLWERLPFVQMVLVLSVTIVVMLGGFLIHDRLRGGAGK
                     K"
     gene            3194..5035
                     /locus_tag="PLL88_07415"
     CDS             3194..5035
                     /locus_tag="PLL88_07415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ01431.1"
                     /translation="MKTESFRRTYLPRILTGLSGIFLLVRSFRYANLLPARMDESLFL
                     YKGLLFANGTYTPFESYGLWTNKNPLSFLIPGWFQLIFGAGLRSGRIFSIMLGLLTIL
                     AIWIVVRKFTNEWLAFFAAAAIALNPFYAWTFAQFISQSLIAALLAWTLVFALGKEKP
                     IWQIILGTILAVLVVQVRQNMLPVIPFLILYIFWENGKKAGTTSLFTALLVFGGLTAV
                     YWPEIIRNYLAVIPPFMQSFALKFINLPDSTLVVANTIQPIERIMSLTQGFRTHFPEI
                     LGAFSACVWLLLPARKKEPTYKVIIFLLILFITLFLAHIWASILKNFCVYCFSNYLAF
                     FGILGIILFALCIDKQEDKTPVFLQICGIIILLILCASAGFSIYQDTGHLLMDLEIPR
                     IKDLSIEPGSVALWTMLSNKFGLAFDELEMIIPTAFGLFFGLLFLILTALYRKIKKVR
                     SSFLASAILLFAILGCLITFPLTELQYSAGLRSCSGDSISAYEAIGEYLDESIEPGAR
                     VWWWGISGQIILSYMDDIHIYPPQLNYMFNYLAGSASDKFYQLGYWNEDLAREWLAEA
                     DYAIVENAVFSGEVVQWIDAKQFDELPPTITINQCSTSDYFRVFRRK"
     gene            5032..6003
                     /locus_tag="PLL88_07420"
     CDS             5032..6003
                     /locus_tag="PLL88_07420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ01432.1"
                     /translation="MMDLSIVVPVYNERENIHLVYEAVHKAIVPLQKKWELILVDDGS
                     KDGSLEEMKAIAKKDAGHVRVIVLRRNFGQTAAIAAGIDNAVGEIIVLCDADMQNDPA
                     DIPMMLEKLSEGYDVVSGWRYDRQDKFLTRVLPSRMANWLISVVTGVHLHDYGCTLKA
                     YRKEILSGFHLYGEMHRFIPVYADAGGARITEVRVHHHARKYGKAKYGLERTFKVLLD
                     LFTVKFLTSYSKKPIYLFGGMGVALILSGALVLLYLFIRRVWFQVGVTTSPWFLISVT
                     SVILGFQAILMGFIAELLVRTYHESQNKPTYTIRTIINGKANSTGKE"
     gene            6010..7155
                     /locus_tag="PLL88_07425"
     CDS             6010..7155
                     /locus_tag="PLL88_07425"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ01433.1"
                     /translation="MRILVLNHEYPPVGGGGGRVSQDLNLGFCKRGHEVKIITPFLQG
                     LPKKEYYPNYEIIRVPSLRKFAFRATLIDMGAYVVSSYLTGSRLIRTWRPDIIHGHFA
                     VPAGAAARMLSKKFRIPYFLTAHLGDIPGGVPEKTGRWFRLFYPFTKKIWKGAQRIIT
                     VSSFTRELAERSYPGIEAELIFNGVDTEIIQPDHLEVHAPPQIVFAGRFMEQKNPLLI
                     IEVLNRLKELPWHCVMMGDGPLKAAVEQKIGECGLQERFTLTGWIDPEQVIAIYRASD
                     ILFMPSRSEGLPVAGVQAIAMGLVPVLSDVGGCADLVIPNESGFLFQPGEADAFTFAL
                     KTLLSDHEKLYRFKKKSRERSLNFDLKRIVTEYEKIMNEILHEQTFA"
     gene            7287..8054
                     /locus_tag="PLL88_07430"
     CDS             7287..8054
                     /locus_tag="PLL88_07430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ01434.1"
                     /translation="MKNKKVDTEVQESYDNLWKHEWRDLHKVGPGVRTRNRILLKYFK
                     KYITSGSVLDAGCGDGSLLIKLHQVYQDSLEYDAGDISEEVLQIIRAQPFIGEIFLLD
                     LEKSSTIPSKTYTAVICSEVLEHLKDWQLAVGYISTLLEKDGFLFITVPAQMKYWGPH
                     DEFALHYRRFEIDEIENYLRQNGYQIKESKYWGWPFYWLYYTLILNRTPPKIVMREVT
                     SPIKRFAANIFFSLFLIDDLFSTRWGRRLFLVAQKMH"
     gene            complement(8104..9021)
                     /locus_tag="PLL88_07435"
     CDS             complement(8104..9021)
                     /locus_tag="PLL88_07435"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOJ01435.1"
                     /translation="MKKPDWKQILKWGGTLVSVGLFIWLLTRLDWQEAWAIARNMPIY
                     TYGAAIFLLVLGQCLNSVRWYTLLRAQDVRITPLETFKIHMGGTFASNFLPSTIGGDS
                     LRLLAVAKITHDQPLSLASVILDRLVNLIATFTLVPFSIGIFRSSGVRFNGDIFGFLI
                     TAVFQGKLWNWIKHTWEKYVEIVRRWLQKPASLGAAFVISWISRIVILGAVWVLSRGV
                     GMHLTLWQVVGVNTVSYFVTLLPITISGYGLRELTLTTLYTMLGATLEQATALTIITR
                     LFSTMITLPGVIWMQRIISETRDIKKETA"
     gene            complement(9032..10249)
                     /locus_tag="PLL88_07440"
     CDS             complement(9032..10249)
                     /locus_tag="PLL88_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOJ01436.1"
                     /translation="MAEHSKERILLPRVIYGNRGDLLSRWGLLNGLRVIGQEAMHVFA
                     HRSADLPEHLRMNYDPYGKLHNVLLSPGAKRALRTADRILWGGGLDMTDESSMAKLMY
                     LLATFSHYRMLGKEIDCVFQGAGPLLTRTGRLIARQILQRVSKFIVRDEYTYDLLSEL
                     NPDPAYFMARDAIFFPGFEQAMLARPRSQVVEGYRGKADTPLIAINIRRWFHFSSDVI
                     PFQLARKRYESRGLDKMQQLIEIYIDLVRKLRLDFNARILLVSAYNPGVYSWEDDMYW
                     LQMVKSAFSTDEAVCVVDYPLDMMDYTVLMASVDLALSMRLHSSLTALRFGKPAVNIS
                     YAPKGVHAFHDLGLEGNAFDINSFMQDPSPVWSRITAILQGGKEQKEFISRKVSESID
                     MNMKALRALFEGK"
     gene            10376..12310
                     /locus_tag="PLL88_07445"
     CDS             10376..12310
                     /locus_tag="PLL88_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01437.1"
                     /translation="MIAVFAFIEIKEIYWGGLDRNVYFSAASLILLALYFVMILLAFG
                     LALCHFLFHWGPYPEKHARWKNLFFFLLGIFPSVFLQFSRWGLVFSSAFLRILLLSSV
                     LFLMVYVLKEKEVLTFRMHLFFPALIFTTACLLLCSRLRMIVDYPFSLSWSEGNRFWD
                     YSLLFGGERYTIAQGSHVAAFLDLGRQSIWGIVFLCKHLSIAGMRAWNVALYFFPPFT
                     LGFLLFKHKGMRLSTWVFYALWTYAFLSEGPIYAPLIFCAILVFLASKLENYPLSLLL
                     IACAGFIANITRFTWILAPVVWAFLLFYFREKGSGTMRRNLKSYGAALAGLIGGVVLP
                     NFIPLEPSSIILGENASVDILYSIKTILQNQDLLWYRLLPSKTFPLGILPALFLIVAP
                     LFIVVGVYLNKEKVQLEKAEKFYFLASLIGFFLIGCVVSVKVGGGSNLHNMDMFLLTI
                     LLVISIFWKNGMAEWFQTEMRKTNLVSLLIFFLLGYPGIRFISSVRPLSLPSSEIVAN
                     AVHAIQSTVDDRKEEGDILFIDQRQLLTFGAIQDVELVGEYEKKLLMNEALSNDQAYF
                     DLFYQDLQSRRFALIVAEPLNIEYQSDENNFGEENNAYVKWVSEPLLCYYEVLKTLQE
                     VGVELLVPRSSPLPENINCP"
     gene            12320..>12630
                     /locus_tag="PLL88_07450"
     CDS             12320..>12630
                     /locus_tag="PLL88_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01438.1"
                     /translation="MNLAYLIPRVIRHFMPEQLADWMKKRHFVIKAGIETMDPRVAVD
                     HYLDYFAQAGIDFQKKSIMIFGYGGNVQIGCEFLRRGAGRVILCERQGLPYSKLTLEL
                     A"
BASE COUNT         3050 a         3227 c         3018 g         3335 t
ORIGIN      
        1 ccccatcaca cctttcatct cgacctccta tcccgaaatc aaccgggcta tcacccaaaa
       61 agcctgggac catgttcaag atttgttgcg caagatcacc accatttctg gagcgtggac
      121 aatcggcgcg tcgctgggac tggcgatctt tggtaaatgg ctcctttctt tatacggagc
      181 cgattttgtg ccggcatatc caaccatgat gatctttttg atcgggctgg ggtttgccaa
      241 catcttcttc tggaatcgcc cgctcctgct ttcgctcaaa cttcccatgg tgccctaccg
      301 cgcaagttta tattgcggca ttgccaaagt tctgctctct ttcttgctgg ttccaacgct
      361 tggtctgaac accgaagcct ggctgctgtc gatctttttc ctgatcagca tcagcgtcat
      421 cgtgctgcaa ggggtgaaag agatcaaaaa acgagcacag ccagaacaaa caggggcttc
      481 attatgagaa tcgcatgcat cacaacctcc cagataccct cccgaacagc caacagcatc
      541 caggctatga aggtctgcca cgcattaaag caatgcgggc aagaaatcaa gctatgggtt
      601 ccattcttca agtcagccga ttggactgaa ctgaaggagc tgtatggact gcaggaggaa
      661 tttcccatcc aatggctccc ttttaaaaaa tcgctcaggc aatatgattt ctgctggcac
      721 gccgtgcagc aagccagaca atggaacgcc gatgtgatct atacctgggc tttgcagagc
      781 gccgtttttt ctcagagaca gcagatacca tctgtcatgg aattccacga ttttcccatg
      841 ggtacttttg gaccccatct tttcaaaatc tacattcgcc accaccagag atgcctttcc
      901 ctcaccacca ccgccgcact tgcccaaggg cttgaagaaa agtacggctt cacttttcca
      961 agcggctctt tgcaaatcgc gccgaacggc accgacccgc agtgttacct gcatctgccc
     1021 agccctgtgc aggcgcgcaa aaaactagga ctgcctgagg gagtcacggt gggattcacc
     1081 gggcactttt acaagggtag aggaattgag ctcttgcttg agatcgcgct cgccttgcca
     1141 gacatccact ttctgtgggt cggcggaaaa gaagagcata tccagccatg gcgagaagag
     1201 ctgaaagcca ggcaaatcaa caacgttacc ctggtggggt tcatacccaa ccaggaactt
     1261 cccttatatc aggcagcctg caacattctc gtgatgcctt acggcaagaa gatctccgga
     1321 agttcaggag gggatatttc gcaggtcatc aaccccatga aaatgtttga ttatatggca
     1381 agcgggcggg ctatcgtagc cagcgatatc cccgtattcc atgaaatcct atcggaacaa
     1441 actgcggtgt tctgcccgcc cgaagatagc cagggctggt cagcagctat tcgcagattg
     1501 gcggatgatc aagacgaacg ggaaaggctg gcgaacaatg ccaaagcgcg ctcagctcaa
     1561 tacagttgga cagcgcgggc agcaaaaaca ttggcattga tcaacgactt ggagggtttc
     1621 aggcatgaag caaggcttga ccaaaaatct tagcgtatcc gttccaatcg cgatcgggtt
     1681 agggggactg gtatgcctgg taaacccggg agaaaaacca ttggtgggtt ggctgggcag
     1741 cgccgttctc tcttttgtct ttcttttcgg gatcgtttac gccattcaaa aattacaaag
     1801 tccgaagaag gtccgcctca tttctttgat caatttttca gtgagggtta tcgccggcat
     1861 cgcgcttttt ctcatgcttc ccatattcgg atatgaggag gaaccccctc aagccggata
     1921 tttatatctg gatgcctacc gcagggatgc gaacgcctgg cagattgcaa gtgacgctga
     1981 acccatcttc accggttttc aggacgagtt ctacacggat caatacggcg gcatgctttc
     2041 gctgagcgcg gtcgtgtatc gtgcattcag ccctgatgcg caccgcccaa tcctgatcct
     2101 tctattgacc tctttcataa gcaccttggg tatccccttt tactggcaag ccctgcaaga
     2161 acgttttggc aacctgacag ccaccatcgc ttgttggatc ctggctttat atcccgaggc
     2221 ggtcatactg ggcggttcgc agatgcgcga accgttgatc atcggtctga gcggcatcat
     2281 tttatgggga ttggaacatc gtaagataaa caaacgcgca ggaatgattg ccctgctcag
     2341 cgcttttctg gcgctcatgg taatatccag ccgatatgcc atcgcagttt cgctgttaat
     2401 cgccatctgg gtatggtctg acctcaccag ccaggacacc gcaaaacaga aaaagatctg
     2461 gcagatttta ccgttggtcg tcatcatcct ggcgggtctc ctggtcagtt gggagtggct
     2521 ggttgacact tcacgctggg acctgtatct catggaaagc agttcgggga gagtgcaatt
     2581 cgaattggat aacatcggag agcaattccg catcccattc ttggttcttt acggcatcct
     2641 gcagccggtt ctgccggcgg caatcgccta cccgggtatt gccatcatgc gcgtcattgc
     2701 cattttccgc tccgccggtt ggtatgccct ggctcctacc ctgctgctgg cgcccttcat
     2761 catgcccaaa gaaacgaaca aaaggaaccg gaaaattttg attctgacca cctgttttct
     2821 tttcttctgg actctgcttt cttctctgcg cgccggaggg gaccaatggg acaacccacg
     2881 ctatcgcaca gccctccttc cctggttcgc cctggttgcc ggctgggttt tcatatattt
     2941 caaagaacat aagaatccct ggctgaaaag agcctatagc atcctcaccg ttttttgtct
     3001 attctttctc caatggtatt tcagccgtta ttacaagctg tgggagcgtc ttccttttgt
     3061 ccagatggtg ctggtgctga gcgtcaccat cgtggtcatg ctcggcggat ttttgatcca
     3121 tgaccgctta cggggcgggg cgggcaagaa ataaagcgcc gatgttataa tcacagatgt
     3181 ctacttggaa cgcatgaaaa cggaatcatt tcgaagaacc tacctcccac gcatcctgac
     3241 aggtctttcc ggcatttttc tgctggtgag aagttttcgt tacgcgaacc tcttgccagc
     3301 gcgcatggat gaaagcctct ttttgtacaa ggggctcctg ttcgccaatg gaacctatac
     3361 cccttttgag tcctatggat tatggaccaa caagaaccct ctcagctttc tcattccggg
     3421 ctggttccag ttgatcttcg gcgccggtct ccgctccggc agaatttttt ccatcatgct
     3481 gggattgctc accatcctgg caatatggat cgttgtgagg aaattcacga acgaatggct
     3541 ggcttttttt gcagcagctg ccattgcttt gaaccctttt tacgcctgga ccttcgcaca
     3601 attcatttcg cagagtttga tcgccgcgct tctggcatgg acgcttgtct tcgccctggg
     3661 taaagaaaaa cccatctggc agatcatact cggcacaatt ctggctgtgc tggttgtgca
     3721 ggtcagacag aacatgctgc ccgtcattcc atttctaatt ctgtacatct tctgggaaaa
     3781 tggaaagaaa gccggaacca ccagcctgtt caccgcattg ttggtgttcg gggggctgac
     3841 cgccgtctac tggccggaga tcatccgcaa ttacctggct gtcattcctc ccttcatgca
     3901 atcctttgct ttgaagttta tcaacctgcc agattcgacc ttggtggtag ccaacacgat
     3961 tcaacccatc gaaagaatca tgtcgctcac ccagggtttc cgcacgcact ttccggaaat
     4021 actgggcgcg ttctctgcat gtgtttggct acttctacca gccaggaaaa aggaacccac
     4081 gtacaaggtc atcatatttc tgttaattct ttttattacc ctcttcctcg ctcatatttg
     4141 ggcttccatt ctaaaaaatt tctgtgtgta ctgcttttcc aactatctgg cattttttgg
     4201 catccttggc atcattttat tcgccctctg tatcgataaa caggaagaca aaacacctgt
     4261 ctttttacaa atatgcggca tcatcatctt gttgatcctc tgcgcaagcg ccgggtttag
     4321 catttaccag gatacagggc atcttctcat ggatttagaa attcctcgca tcaaggacct
     4381 atctattgaa cctggttcgg ttgcgttatg gaccatgctc tccaataagt ttggattagc
     4441 atttgatgaa ttggagatga tcattccgac agcgttcggg cttttcttcg gactcctttt
     4501 tctcatcctc actgcgttgt atagaaaaat caagaaagtc agatcttctt tcctcgcttc
     4561 agccatcctc ctctttgcaa tcttgggatg tctgataacc tttccgctga ccgagttgca
     4621 gtacagcgcc ggattgcgaa gctgttcagg tgactccatc agcgcatacg aagccattgg
     4681 cgagtacctg gatgaatcca ttgaaccggg tgccagggtt tggtggtggg gcatttcagg
     4741 gcaaatcatt ttaagttaca tggatgatat ccacatctac ccgccccagc ttaactacat
     4801 gtttaattat ctggcgggaa gtgccagcga taaattttat caacttggat actggaatga
     4861 agatctcgcc cgggagtggt tggctgaagc ggattatgcc attgttgaaa atgctgtgtt
     4921 cagcggcgaa gtcgtacaat ggatagatgc aaaacagttc gatgaactgc cccctacgat
     4981 cacaatcaac cagtgcagca caagcgatta ttttcgcgtt ttcaggagaa aatgatggat
     5041 ctttcaatcg tggtaccggt ctataacgaa cgggaaaata tccatctcgt ttacgaagca
     5101 gtacacaaag cgatcgtccc cttgcaaaag aagtgggaat tgatcctggt ggatgatggc
     5161 agcaaagacg gcagcctcga agagatgaaa gccatcgcaa aaaaggatgc cgggcatgtg
     5221 cgcgtcatcg tcttacggcg caacttcggt caaaccgctg ccatcgccgc cggcattgac
     5281 aacgcggtgg gagagatcat cgttttatgc gatgcagata tgcagaacga cccggcggat
     5341 attcccatga tgctggaaaa actatccgaa gggtacgacg tggtcagcgg gtggcgctac
     5401 gaccgccagg ataaattttt gacccgcgtt ctgccttcca gaatggcaaa ctggctcatc
     5461 tccgtggtga ccggcgttca tctgcacgat tacgggtgca cgttgaaagc ttaccgcaag
     5521 gagatcctat ctggattcca tctttatgga gaaatgcacc gtttcatccc ggtctatgcc
     5581 gatgcaggcg gcgccaggat cacagaagtg cgcgtgcacc accacgcccg caagtacgga
     5641 aaagccaaat atggcctgga gcgaacgttc aaggtcctct tggatctttt caccgtaaaa
     5701 ttcctcacct cctattccaa aaaacccatc tatctctttg gcggcatggg ggtcgccttg
     5761 atcttgtcag gggcgctggt cttgctgtat ctcttcattc gcagggtctg gttccaggtg
     5821 ggggtaacaa catctccctg gttcttgatc agcgtgacca gcgtgatctt gggttttcaa
     5881 gccatattga tggggttcat cgccgagctg ttggtaagga cctaccacga atcccagaac
     5941 aaacccacct acaccatccg caccatcatc aacggcaaag cgaattccac cgggaaagaa
     6001 taaattttta tgcgcatttt ggtactcaat catgaatacc cgccggtggg tggcggtggc
     6061 ggcagagttt cacaagacct caacctgggt ttttgcaaac gtgggcacga agtcaaaatc
     6121 atcacgcctt ttttacaagg tttgcccaaa aaagaatact atcccaatta tgagattatt
     6181 cgcgtaccat ctctaagaaa attcgctttt cgggcgaccc tgatcgacat gggagcttat
     6241 gtcgtaagca gctacctcac aggcagccgg ttgatccgca cctggcgacc ggatatcatc
     6301 cacggtcatt ttgccgtccc ggcgggcgca gccgccagaa tgttgtcgaa aaagttccgc
     6361 attccgtatt ttctcaccgc ccatctgggt gatatccccg gcggtgtccc ggaaaagacc
     6421 ggaagatggt tccgtctctt ttacccattc acgaaaaaaa tctggaaggg cgcgcaacgg
     6481 atcatcaccg taagcagttt cacccgagag ctggctgaac gcagctaccc gggcattgaa
     6541 gcggagctga tcttcaacgg ggtggacaca gaaatcatcc agccagatca cctggaagtt
     6601 cacgcgcccc ctcagatcgt gtttgccggg cgcttcatgg aacaaaagaa tccgctcctg
     6661 atcatcgagg tgctcaacag gctcaaagaa ttgccctggc attgcgtcat gatgggtgat
     6721 ggccctctca aagcggctgt tgagcaaaag atcggtgagt gcggtttgca ggagcgcttc
     6781 acattgacag gttggatcga tcccgagcag gtcattgcga tctatcgagc cagcgatatc
     6841 ttgttcatgc cctcccgctc tgaagggttg ccggtggcgg gggttcaagc cattgcgatg
     6901 gggttggtgc cggtattatc ggatgtgggt ggctgcgctg acctggtgat accaaatgaa
     6961 agcgggttcc tatttcaacc gggagaggcg gatgcgttca cctttgcatt gaagaccttg
     7021 ctttccgatc atgaaaaatt atatcgattt aaaaagaaaa gtcgcgaacg gtcgttgaat
     7081 tttgacctca agcgtattgt aacggaatac gaaaagatca tgaacgaaat cctccatgag
     7141 cagacgttcg cctgaattct tatgatcccg ctcgtgtaaa aagagaaaga aacctgcaat
     7201 gaatcgtgcg gttttttcag tttgttgcca tcgatcatgg ttcagtcagt aatacattgg
     7261 gctcgttcat gataaaattt cccacagtga aaaataaaaa agtcgacacc gaagtccaag
     7321 agagctacga caatttatgg aaacacgagt ggcgtgatct tcataaagtc ggaccagggg
     7381 tccgcacgcg taaccgtatc ctgttaaagt attttaaaaa atatataacg tccggttcgg
     7441 tcctcgatgc aggctgcggc gatggcagtt tactcatcaa attacatcaa gtctaccagg
     7501 actcgcttga atatgacgcc ggggacattt cggaagaagt gttgcagatc ataagagcgc
     7561 aaccgttcat tggcgaaatt tttttgctgg acctggaaaa aagttccacc atcccttcaa
     7621 aaacttatac agcggtgata tgttccgagg tcttagaaca tctaaaagat tggcaacttg
     7681 ccgttggtta catctcaacg ctgcttgaaa aagatggttt tctgttcatc accgtcccgg
     7741 cgcaaatgaa gtattgggga cctcacgatg aatttgcctt acattaccgg cgttttgaaa
     7801 tcgacgagat cgaaaattac ttgaggcaga atggttatca gataaaggaa agtaaatact
     7861 ggggttggcc tttttattgg ctctactata ccttaatcct caaccgcact cccccaaaaa
     7921 tagttatgcg cgaggtaaca tctccgatca aacggttcgc agccaatatt tttttctcgc
     7981 tttttctcat tgacgacctt ttctccacaa gatggggaag aaggctattc cttgttgccc
     8041 aaaaaatgca ttgattcatt tctggggaaa ctcgcttgtg actgttttag gaacgctggg
     8101 gtatcatgct gtctcttttt tgatatccct tgtctcggaa ataatgcgct gcatccagat
     8161 aacgcccggt agggtgatca ttgttgaaaa aagacgggtg ataatggtaa gagccgtagc
     8221 ctgttcaagc gtagcgccca gcatagtgta gagggtcgtc agggtcaact cccttaaccc
     8281 atagcccgat atggtgattg gcagaagagt aacaaaatac gaaactgtgt tcactcctac
     8341 cacctgccag agagtgagat gcattccaac gcctctcgaa agaacccaca ccgcacccaa
     8401 gatcacgatc cttgagatcc aggagatgac gaacgccgca cccaaggaag ctggtttttg
     8461 caaccagcgc cgaacaattt ccacatattt ttcccatgta tgctttatcc agttccacaa
     8521 cttgccctgg aagactgccg tgatcaaaaa cccaaagata tcgccattaa atcgcacgcc
     8581 gctcgatcga aagatgccga tgctgaatgg caccagagtg aacgtggcta tcaggttgac
     8641 cagtcgatcc aagatcaccg atgcaaggct caatggctgg tcgtgggtga ttttggcaac
     8701 agccaaaagc cgcaagctat ctccgccaat ggtcgagggg aggaaattgg acgcaaacgt
     8761 cccacccata tggattttga aggtttccag gggtgtgatt ctcacatcct gggcgcgcag
     8821 cagggtatac cagcgaaccg aattgaggca ctgccccaaa acgagcaaga atattgccgc
     8881 tccataggta tagataggca tatttctggc tatcgcccag gcttcttgcc aatccaggcg
     8941 ggtaagaagc cagatgaaca acccaaccga aacaagcgtc cccccccatt ttaaaatttg
     9001 cttccaatcc ggttttttca ttgggtatgc atcatttccc ttcgaagaga gcgcgcaatg
     9061 ccttcatatt catgtcgatg ctctcactca cctttcgaga gataaattct ttttgctctt
     9121 ttccaccctg caagatcgct gtgattctgc tccagacagg agaaggatct tgcatgaagc
     9181 tgttaatgtc aaaagcgttt ccttccaaac cgaggtcatg gaaagcatgc acgcctttgg
     9241 gcgcgtaact tatattcacg gcgggcttgc caaagcgcag cgcagtgagg ctggaatgta
     9301 agcgcatgct aagcgccaga tctacgcttg ccatcagaac ggtgtaatcc atcatatcca
     9361 aaggataatc caccacacaa actgcctcat ccgttgaaaa tgcgctcttc accatttgca
     9421 gccagtacat gtcatcttcc cagctataca cgcccgggtt ataggcagac accaacagaa
     9481 tccgcgcgtt gaaatccaat cgcagtttac ggaccaggtc gatataaatt tcgatcagtt
     9541 gctgcatctt atcaagaccc ctgctttcat agcgctttct tgccagttgg aacgggatca
     9601 catctgaaga aaaatggaac cagcgcctga tgttgattgc aatcagtggg gtatccgcct
     9661 ttccacgata tccttccacc acctgggatc tcggacgggc gagcatggct tgttcaaaac
     9721 cgggaaagaa aatggcatcg cgcgccataa aatacgcagg atcagggttc aattcagaca
     9781 acaaatcgta tgtatactca tcgcggacga tgaacttcga tacccgttgc aggatctggc
     9841 gcgctatgag cctgccggtt cgcgtcagca gcggaccagc cccttgaaag acgcaatcga
     9901 tttcctttcc cagcatgcgg tagtgagaaa aagtcgccaa aagatacatg agcttagcca
     9961 tgcttgattc atcggtcata tccagtcccc cgccccagag aatgcggtcc gcagtgcgca
    10021 atgcccgttt cgcgcctgga gataacagca cgttatgcag ctttccatag gggtcgtaat
    10081 tcattcgtag gtgttctggc agatcggcgc tgcgatgggc gaaaacatgc atcgcttcct
    10141 gccctatcac acgcaagcca ttgagcaatc cccatcggct gagaagatca ccccgattgc
    10201 catagataac ccttggaagt aatatccgtt ccttcgaatg ctcagccatt ttctacccag
    10261 gcgacacgtt tttccatcga gtttataatt gtatcaaatt tacactgaaa gaatgatgat
    10321 gacagccgac aaatcctcta ctttgcagaa catagttctc ctactgttga catttatgat
    10381 tgccgttttc gccttcatag aaattaaaga gatctattgg ggtggcttag acaggaatgt
    10441 ctattttagt gccgcttctc tcattctttt agcgctgtat tttgtcatga tcttgcttgc
    10501 ctttggattg gcattgtgcc atttcttgtt tcattgggga ccttatcctg agaaacatgc
    10561 tcgctggaaa aatcttttct tcttcctcct gggaattttt ccatcagttt ttcttcaatt
    10621 ctcaagatgg gggctcgttt tcagcagtgc ctttctcagg atcttgttgt tatccagcgt
    10681 tctctttctc atggtatatg tactgaaaga gaaagaagtt ttaactttta ggatgcatct
    10741 ctttttcccc gctctcatct tcaccacagc ctgcctgtta ttatgcagcc gcttacgcat
    10801 gatcgtggat tatcccttct ctctctcctg gtcagaagga aatcgttttt gggattattc
    10861 tctccttttt gggggagagc gctacaccat cgcacaaggt agccatgtgg cagcttttct
    10921 ggatcttggc aggcaaagta tctggggtat tgttttctta tgcaagcatc tctccatcgc
    10981 cggaatgcgc gcgtggaacg tggcgcttta ttttttcccg ccgttcaccc tgggattcct
    11041 cttgttcaaa cacaaaggga tgcgactttc tacctgggta ttttatgcgt tatggacata
    11101 tgcatttctc agcgaaggtc cgatctatgc cccattgatc ttctgcgcca ttcttgtatt
    11161 ccttgctagc aagcttgaga actacccgct ttcattactt ctgatcgcct gcgccgggtt
    11221 catcgcgaac atcacccgtt tcacctggat cctcgctcct gttgtctggg cattccttct
    11281 cttttacttt cgcgagaagg ggtcaggcac catgcgcaga aatctcaaaa gttatggagc
    11341 tgccctggct ggattgatcg gcggggtggt gttgcctaac ttcatcccgt tagaaccatc
    11401 ttcgataatt ctgggagaga acgcctctgt agatatcctg tattcgatca agaccattct
    11461 tcaaaatcag gatctgcttt ggtatcgctt gcttccctcc aaaaccttcc cgcttggaat
    11521 tctcccggca ctttttctga tcgtagcccc attattcatt gttgtaggtg tgtacttaaa
    11581 taaagagaaa gtccaattgg agaaagcgga aaaattctat ttcctcgcct ccctcatcgg
    11641 atttttcttg atcggttgcg tggtaagcgt gaaggtcggc ggggggagta acctgcacaa
    11701 tatggacatg ttccttctga ccattctatt ggttatcagc atcttttgga aaaacggaat
    11761 ggctgaatgg ttccagacgg aaatgcgtaa aaccaacctg gtgagcctgc tgattttttt
    11821 cctgctcggc tatcccggca tccgtttcat ctccagcgtg agaccgcttt ctctgccttc
    11881 cagcgagatc gtcgcaaacg ctgtccatgc catccagagc actgtggatg atcgaaagga
    11941 agaaggggat atcttgttca tcgaccagag gcagttgtta actttcggcg cgatacagga
    12001 tgtggaactg gtcggcgagt atgagaagaa gctgctgatg aacgaggcat tgagcaacga
    12061 ccaagcttac tttgaccttt tctaccaaga tctgcaaagc cggcgtttcg ctctgatcgt
    12121 tgcagaaccc ctgaatatcg aataccaaag cgatgaaaac aattttggag aagagaacaa
    12181 tgcctatgtg aaatgggttt ccgaaccttt gctgtgttat tatgaagtat tgaaaaccct
    12241 gcaagaggtg ggggttgaac tgcttgttcc gcgatcatca cccctccccg aaaatattaa
    12301 ttgcccctag gagatagcca tgaatcttgc gtatctcatt ccgcgcgtta tccggcattt
    12361 catgcccgaa cagcttgccg attggatgaa gaagagacat ttcgtcatca aggctgggat
    12421 cgagaccatg gatccgcggg ttgctgtgga tcattatctg gactacttcg ctcaagctgg
    12481 tattgacttc cagaaaaaaa gcatcatgat ctttggttac gggggaaatg tgcagatagg
    12541 ctgcgaattt ctacggcgcg gggcggggcg ggttattctc tgcgagcgcc agggtcttcc
    12601 ctactccaaa ttgaccttgg aacttgccag
//
LOCUS       DAOSDS010000055        12622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1926_length_12622_cov_4.234742, whole genome shotgun sequence.
ACCESSION   DAOSDS010000055 DAOSDS010000000
VERSION     DAOSDS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12622
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1926_length_12622_cov_4.234742"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4471
                     /locus_tag="PLL88_07455"
     CDS             <1..4471
                     /locus_tag="PLL88_07455"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ01439.1"
                     /translation="LEVGKGTFVQSLVADELKRASGDAQVGSHQHLSDALEQLQEFNY
                     LADVYYLAEQETNSVNELDQLLQQLDVISQSVLTKKEAVLSERETKQKVLPGYNDVAC
                     AYNWKTMPQLGKTLDLKKPEIKEIEAYLKDIEEQISNNPKDAMVLRQVADFYGSLGDH
                     HRTLHYLRLAKILDADDQDLNQKLFELAMADHRWQDALIQVKEKKQAADEQDLTLTQF
                     YLECKALLENKERDTVRQRLEGMPTDLSSMDAVSSLRMGELWMDVEGWERAQLCFKHA
                     ILNGSTDYAAWINSYRCLLKSGHRDEAQQILSQAFELFHERKDFYETSILAMLECGEE
                     DRGLALLEKIDLEHGCPDAIAGIIQYVEKKGCGNHAYELAMKAISHFPLNAKLGMVTA
                     QVLMENGESEQAGKYLKLVKDEKEKDHDYILLDAIALLKSSPSIFPLGTQRVSIDELD
                     SIAHKIDPLPEGDYWKELVKAEVHYLEDNTQGAISKYKELILKNSLNANRENLWRAQA
                     GLAKAMMKINQTETAITLLNEALRSNPENLAIYDLLVAAYGNKNLNQEAVELIRKAYK
                     TCSSNKRITGWYVDQMLKFGQTEEVRKYFSKEAAQNHSSIEFLIEKMRFENQFGSQDE
                     TKASIHALLLLERIPTQDLHTMLKVAQDADFKDLSLKIIQRLQKMNEDDPETCLMKAS
                     VYWSQGEHATAVDCLDQLGKDKTWNLLKNAVVSLADDHTENPEALLQTLKNRQDVNAI
                     VQNLPEHVHSVLPSEWISAFSSDEIWVKKLLLSFLTANNKDGNLAFPDSSGDLQIHDV
                     QTQAYLAIWHWYAKGNADNYDWIDILERLKRSVDQQKIIPLIGIVLNILLENGNEIAA
                     AALIDALPDIYSSIEPNTTFAKARVLQKNHNHSDAQIVYQRALGMIQEVQNDSCDLDA
                     SILAVFTTLPIWKANCAFEMGDWETAVRAYFYCLGNANNLPALKEMCVNRLLVMGFKE
                     WSYGKTGANRNIPKIFAQNEFHSLSEQITLLPQEKQSAYLAGHNFLINGTCSVFEREQ
                     DGLIGSVCKLLDACQRGQYEEAIQILETNPKETDLPLVALGMFPKEKYRDLIPAFHAA
                     MIADKKNAFLSAGLAQIFIHENETDVAIDALETAVGLIDREATWHRQLAMLYEEKKEL
                     QKATVCWERAVSLDPENEDAKKRYLQSLLLNKEFSNGIGVFEKDKELFTGDSAILREI
                     VNAYYETGELRKASGYLSALKNAGKEDLELLLIQARIAEKLENIPKAMELIRDAYRLD
                     PKSPEVIVELAKIKSLEEDEGFGLEIIEKALESDIINDQLILEKTAYLERIRGKKRAI
                     DFLEGYLEKAANPDTSLLNRYADLLFGTGNKDESLKIYESSIQKDENQSEIHRIIGML
                     SMKNGNLDNALFHLNKAIQQDPHDMEAYLALTDVLINRRETKRAEKIIQTAIERCKEH
                     YLIYEKASKVYNQLNDSEKAEIFLRKATALNPMDDDLREKLGIILEKRIFNKE"
     gene            4486..6903
                     /locus_tag="PLL88_07460"
     CDS             4486..6903
                     /locus_tag="PLL88_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOJ01440.1"
                     /translation="MKITRKDLLKWESTASTAEEQSLLLDCLKTWNETFPGDLEIKCE
                     YGRSLIAAGDTVAGEEKLKEVLAVHPEYERAYEALYKNSKDEEERNLYHEFLSFFQGG
                     TSQVAPNGWADRLTEIKSALSEGELGAVEKDVLELMQKFPDNVLFPIYYLGVVLKTKD
                     DYSTLQFAKLYHEKWRKCVFFMLIYAEALLKFGSEIEAVSLLREAVYQDHLGLVANAM
                     WGSNHEYKHLWPDTAEFSIEKTDNEVLSNLLVKEPQVSEQKKTIKRRVEENPDVPPKA
                     FFAKPVYVILASRKGIEKKYGENTFNVILNLVQELTAAINLHKNWSALYFLPDDGNNV
                     LQYGLTTIESNDPWQIKLALHDLSAYLHDHGKKIGAVLIIGGDDVVPFHHLPNPTDDS
                     DSEVLSDNPYGSDDNNYFIPEWAVGRFPDEKGSDPNLLLKQLRQTIQWHKNNTKKRSF
                     AEKVADSLKVWDSIAGFFQEITETKSNYGYSTAIWQRSSISVFRPVGKANLLRVSPPY
                     NANNLDAISMKEAEYAYFNLHGLDDSPNWYGQKDISNTDSTPDYPVALQPGLIDLAAK
                     NPTVVVSEACYGAYILGKTNEDAISLRFLTSGCRAFIGSMCIAYGSVYPPLIGADQFT
                     YLFWNLIEKNYSVGDAFVRAKLNLVKSILRKQGYLDGEDQKTLLSFVLYGDPLYYSDS
                     QAEDHLIQDQEKIVDSYQVIRDQDLDTVAVPKVSSDILMNIKEVVKEYLPGIESAELS
                     IREKQMKVINMVRDAKGAVTMERQTAGRVFLTYKKSFQIQNKTLKQFTRVTLDDRGKM
                     IKFSVSK"
     gene            6991..9720
                     /gene="alaS"
                     /locus_tag="PLL88_07465"
     CDS             6991..9720
                     /gene="alaS"
                     /locus_tag="PLL88_07465"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOJ01441.1"
                     /translation="MQNKSDGNTIRKTFIEFFEKRGHTFVPSSSLVPGGDQTLLFTNA
                     GMVQFKDVFLGLDDRAYTRAVNSQKCMRVAGKHNDLEDVGRDDIHHTFFEMLGNWSFG
                     DYYKQEAITWAWELLTEVWKLDKTTLWVTCFKDESGEIPSDDEAISAWEMQPGIDQSH
                     ILQFGRKQNFWEMADMGPCGPCSEIHIDRGEEFCNKRNDPDHVCAVNGSCTRFVELWN
                     LVFMQYNRISPKKLVPLPKKHVDTGMGFERIVSVLQGVDSNYKTDLLLPLMDVVLRLT
                     GASVEEREKNVTPYRVIADHARAATFLISDGVVPGNIGRNYICRMIIRRAARFGTKLG
                     LHEPFLAGVAHEVIRIYRDAYPDLVKNQETILDNLTREEQRFKKTLDTGLSYLSQIME
                     ENKKTKLIAGETAFELYATHGLPFEITRDIAGEEGYLVDEEAYREAMERHRVESGAGK
                     EFGPLGGEDAERYASFFNEIYDAGWLPKEGVTYDPYDIFPTEQPVSALIKDGKLVESV
                     SEGDTVEVVLAKTNFYIQSGGQVADKGKLVSKDGKWEISIEDVKKPTAGLIVHAGKVI
                     AGSPQTGDVAVLEVDARRRQDIMRNHTATHLLHAALRKILGVHVRQAGSLVAPDRLRF
                     DFTHPEALTREEIQKVQDFVNQAILDNEPLHKEEKDLQQAIEEGAMALFGEKYGERVR
                     TVVIGENAGTSYELCGGTHVDTTGEIGNFIIVSEGSVAAGIRRVEAVTGREAYALIQK
                     RLLRLENTAEKLQVGVDQLEKKIESVLDHEKQLEKESSALQKNAVFQKYRDQKNSGEI
                     IGASRILSLAFGPTNGSILLELSDLFRNENNSGIAIFSGINEKDEPFFMVTVTDDLVK
                     KGIHAGEIVNTAASVVGGRGGGRPNLAQAGGKDASKINEALQKAKDYARGKLE"
     gene            complement(9751..11214)
                     /gene="gcvPB"
                     /locus_tag="PLL88_07470"
     CDS             complement(9751..11214)
                     /gene="gcvPB"
                     /locus_tag="PLL88_07470"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOJ01442.1"
                     /translation="MAEPTIYELSSSARKGFQFHDPDVPVSVIPAELKRDDLPFPEVS
                     EVDVIRHFTHLSHLNYSIDSGFYPLGSCTMKYNPRINEELARLPGFAMTHPLQPIETI
                     QGNLALIYQLQEWLKEISGFAGITLQPAAGAQGEFVGGLIIRAYHLSRNDLKRTKILV
                     PDSAHGTNPATSSMSGFDVMNVPSDSRGNIDLEKLKEVCDDCVAGLMLTNPNTLGLFE
                     EHLKEVIDIIHQRGGLVYGDGANFNALLGIIKPGEIGFDIMHFNLHKTFSTPHGGGGP
                     GSGPVAVAPHLVDFLPSPIVGIVEEGDEKTPPLYGFIRPAQSIGQMKSFYGHFGVLIR
                     AFTYIAMHGSDGLRKISENAVLNANYLKKLLENIYTVPFDRKCMHELVVEGFWEDAPG
                     IHALDIAKRMMDYGVHPPTNYFPLIVKEALMIEPTETESKQEVERFAEIMKKIAEEAH
                     KEPELLHSAPHGTPIGRLDEVKAAKDLVLCCWFPQNE"
     gene            complement(11207..12559)
                     /gene="gcvPA"
                     /locus_tag="PLL88_07475"
     CDS             complement(11207..12559)
                     /gene="gcvPA"
                     /locus_tag="PLL88_07475"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOJ01443.1"
                     /translation="MFTPHTDAERKEMLTALGMKSIEELFSDVPERYRFPTLDLPASM
                     SEMFAREYIEGFADANASTKEFVCFLGAGAYDHYIPAAVDSILRKGEFFTAYTPYQSE
                     ISQGTLQAIFEFQSLICNLTALEVSNASQYDGATAAAEACITAYSHFREKRKKIILSH
                     ALNPQYREVIRTYLSGIQNIDIVISGNPDEPFSDPMAMKDLIDEDTALVIVQYPDFFG
                     KIGDPAELITIAHHHGALVCMAVNPIALGLLKTPGEMGADIAVGEGQPLGIPLSFGGP
                     YLGFIAARKELVRKLSGRLVGETVDEHGQRGYVLTLTAREQHIRREKATSNICSNQGL
                     MALAATVYMSLLGKSGMQQVSNLCYQNAHYAAKKIAALEGFKVLTPEPFFHEIVVQCP
                     SSVDHLNHHLLEEGILGGFALENLYPALKDCMLLAFTEKTNKEIIDVLCDILKENNHG
                     "
BASE COUNT         3670 a         2676 c         3151 g         3125 t
ORIGIN      
        1 attagaggtg ggaaaaggca ctttcgttca atctttggtc gctgatgagc ttaagagagc
       61 aagcggggac gcccaggtgg gatcccatca acacttgagc gatgcactgg aacagttgca
      121 ggaattcaat tatcttgcag atgtgtatta tcttgccgag caggaaacaa attctgtcaa
      181 cgaattggac cagttattac aacaattgga tgttatcagc cagagcgttt tgacaaagaa
      241 agaggctgtg ctttccgaaa gagaaacaaa acaaaaagtt ctccctggct acaacgatgt
      301 tgcatgtgcc tataactgga aaactatgcc gcaattgggc aaaacgttgg acttgaagaa
      361 accagaaatc aaagagattg aagcgtattt gaaagacatc gaagagcaaa tttcaaacaa
      421 tcccaaggat gcgatggttc ttcgacaagt ggcggatttt tacggttcac tgggtgatca
      481 tcatcgcacg cttcattatt tacggttagc gaagatcctg gatgccgacg atcaagacct
      541 gaaccaaaag ttatttgaac tagccatggc agatcatcgc tggcaagatg ctttaataca
      601 ggtgaaagaa aaaaaacaag cagcagacga gcaggattta acccttaccc agttctatct
      661 tgaatgcaaa gcactgttgg aaaacaagga gagagatacg gtgcggcaac gtttggaagg
      721 gatgccaact gatctttcga gcatggatgc cgtatcatcc ttgcgcatgg gggagttgtg
      781 gatggatgtg gaggggtggg aaagagccca gctctgtttc aaacatgcga ttttaaatgg
      841 cagcaccgat tatgcagcct ggatcaattc atatcgttgt cttcttaaaa gcggtcacag
      901 ggatgaagca caacaaatac tttctcaggc gtttgaactc ttccatgagc gaaaagattt
      961 ttatgaaacg tcaattctag ctatgctgga gtgtggtgaa gaagatcgtg gcttggcgtt
     1021 gcttgagaaa atcgatctgg agcatggttg tccggatgca atagccggta ttatccaata
     1081 tgtagaaaaa aagggatgtg gcaatcatgc ttacgaactc gccatgaaag caatctccca
     1141 ttttcccctt aatgcaaaat tgggaatggt gactgcgcag gttcttatgg aaaacggaga
     1201 gagtgagcag gctggcaagt acctgaaatt ggtgaaagat gaaaaagaaa aagatcatga
     1261 ttatattctg ctcgatgcta ttgcactgtt gaaaagcagc ccctcaattt ttcccctggg
     1321 aactcagcgg gtaagcattg atgaattgga ttccattgct cacaaaattg atccccttcc
     1381 tgaaggtgac tattggaaag agttggtaaa agccgaggtc cattatttgg aggataacac
     1441 ccagggagcg atctcaaaat acaaagaatt gattctaaag aactcgctta atgcgaatcg
     1501 agaaaacctg tggcgtgcac aagccggatt ggcgaaagcc atgatgaaga tcaatcaaac
     1561 tgaaactgcc ataacgcttt tgaatgaagc gctgcgctcc aatccggaaa acctggcgat
     1621 ttacgatcta ctcgtggctg catatggcaa taagaacctc aaccaggaag cagtggagct
     1681 gattagaaaa gcatacaaaa cctgctcttc aaataaaagg atcactggct ggtatgtcga
     1741 tcaaatgctc aagttcgggc agactgaaga ggtgcgcaaa tatttttcaa aagaagcagc
     1801 ccagaaccat tcctcgatag aattcctgat cgagaaaatg cgtttcgaga accagtttgg
     1861 ttcgcaagat gaaaccaaag cgagcatcca tgcgctactt ttattggaaa gaatacccac
     1921 acaagacctg cacacgatgt taaaggttgc ccaggatgcg gatttcaaag acttgagctt
     1981 gaagattatc caacgactgc aaaaaatgaa cgaggatgat cctgaaacct gcctcatgaa
     2041 agcaagtgtt tattggagtc agggagagca tgccacagcg gttgattgtt tggaccaatt
     2101 gggcaaggat aaaacttgga atcttctcaa gaacgcagtc gtgagcctgg cagatgacca
     2161 taccgaaaat ccagaggcat tgctccaaac actgaagaac aggcaggatg taaacgccat
     2221 tgtacagaac ttgcctgaac atgttcacag cgttttgccc tctgaatgga tcagcgcatt
     2281 ttcatctgat gaaatctggg tcaaaaaatt gctgctcagt tttcttaccg caaacaataa
     2341 ggatggaaat ttagcgttcc cggattcttc tggagatctt cagatccatg atgttcaaac
     2401 ccaggcgtac cttgcaatct ggcattggta cgcgaaaggg aatgccgata actatgattg
     2461 gattgacatt cttgaaaggc tgaagagatc ggtcgatcag caaaaaatca tcccattgat
     2521 aggaatcgtt ttaaacatac tcctcgaaaa cgggaatgaa atcgctgcag cagcgctgat
     2581 cgacgcttta cctgatatat attcatcaat tgaaccaaat acaacgtttg caaaagcgcg
     2641 agtgctccag aaaaatcata atcatagcga tgcacaaatt gtgtatcagc gagcactagg
     2701 aatgattcaa gaggtacaaa atgactcatg cgatcttgat gcgagcattc tggctgtttt
     2761 tacgaccctc ccaatttgga aagcaaattg cgcttttgaa atgggcgatt gggagactgc
     2821 tgttcgggca tatttctatt gcttgggcaa cgcaaataac cttcctgcac tgaaagaaat
     2881 gtgtgtcaac aggctgctcg taatgggttt taaggaatgg agttatggca agaccggagc
     2941 aaatcggaat attccaaaaa tattcgctca aaacgagttt cacagcctgt cagaacagat
     3001 aacacttctg ccgcaagaaa agcaaagcgc ctatctggct ggacacaatt ttctgatcaa
     3061 tggaacttgc tcagtatttg aaagggaaca ggatgggcta ataggttccg tttgcaaatt
     3121 gctggatgcc tgccagcgcg gacagtatga agaggcgatc caaatactgg aaacgaatcc
     3181 gaaagaaaca gacctgccac ttgttgctct gggtatgttt cccaaggaaa aatatcgtga
     3241 tctaatcccc gctttccatg cagccatgat agctgataag aaaaatgcat ttctatccgc
     3301 tggtcttgcc cagatattca ttcatgaaaa tgagacagat gtggctatcg atgcgcttga
     3361 gacggctgtg gggttgatag accgcgaagc gacctggcac aggcaactgg ctatgttata
     3421 tgaggagaaa aaagaactac agaaggcgac cgtctgctgg gagcgagcgg tgagcttgga
     3481 tccagagaat gaagatgcaa aaaaaagata cctgcaaagt ctgctcttaa acaaggagtt
     3541 ttctaacgga attggcgtct ttgaaaaaga caaagaatta tttacaggcg atagtgcaat
     3601 cttgagagaa attgtcaatg cctattatga gaccggagag ctccgaaaag cttcagggta
     3661 cctatctgcg ttgaagaatg ccggcaaaga agacctcgaa ctgcttctca tccaggcgcg
     3721 catcgctgaa aaattggaga acattccgaa agcaatggaa ttgattcgcg atgcttatcg
     3781 gcttgatcct aaatctccag aagtgattgt tgagctggcg aagatcaaat cgctggaaga
     3841 agatgagggc tttggtttag agatcattga aaaagcgctg gaatcagata tcatcaacga
     3901 tcaattgatt cttgaaaaaa cagcttatct ggaacgaata cgcggaaaaa aaagggcgat
     3961 agatttcctg gaagggtatc tggaaaaggc cgctaaccct gacacatcat tgttaaaccg
     4021 ctatgcggac ttgctatttg gaactggaaa caaggacgaa tctctcaaga tctatgaaag
     4081 ttccatccag aaggatgaga atcaatccga aatccacagg ataatcggaa tgctctccat
     4141 gaaaaatggg aatctggaca acgcgttatt tcatctaaac aaggctatcc agcaagaccc
     4201 acatgatatg gaagcctacc tggcattaac cgatgtgctg ataaaccggc gtgagacaaa
     4261 acgggcagaa aagatcatcc aaacagctat tgagcgatgt aaagagcatt atttaatata
     4321 tgaaaaagca tctaaagtat ataatcaact aaatgactcg gaaaaagcgg aaatctttct
     4381 gagaaaagcg actgctttga atccgatgga tgatgatttg cgtgaaaagc ttggtatcat
     4441 cctggaaaaa cgaattttca acaaagaata aggactcatg cttccatgaa gatcaccaga
     4501 aaagaccttc tgaaatggga atcaacggct tccacggcag aagagcaatc attattgttg
     4561 gattgtctaa aaacatggaa tgagactttt cctggtgacc ttgaaattaa atgtgaatat
     4621 gggcgttctc tgattgctgc gggcgatact gttgcgggtg aagaaaaatt gaaagaagtc
     4681 cttgcggttc atccggagta tgagcgtgct tatgaagcac tgtataaaaa cagcaaagac
     4741 gaagaagaac gcaatttgta tcatgaattt ctatccttct ttcaaggagg gacatctcag
     4801 gtagccccaa atggctgggc tgacaggttg accgagatta aatctgcact ttccgaaggt
     4861 gaattgggag cggtggaaaa ggatgtgctg gagctgatgc agaaatttcc ggataacgtt
     4921 ctctttccca tttactatct gggcgttgtg ctgaaaacga aagatgatta ttccaccttg
     4981 cagtttgcca aactgtatca tgagaaatgg cgcaagtgcg tttttttcat gctgatttat
     5041 gccgaagcgc ttttaaaatt cggttcagag attgaggcgg tcagtctgtt gcgcgaagcg
     5101 gtatatcagg atcatcttgg attggttgcc aatgccatgt gggggtccaa tcatgagtat
     5161 aagcacctct ggccggatac cgcagaattt tccatcgaaa aaacagacaa tgaggtgctt
     5221 tcaaaccttc tggtaaaaga gccgcaggta agcgaacaaa agaaaacgat aaaaagaagg
     5281 gtagaagaaa atcctgatgt gccgcccaaa gctttttttg ccaaacctgt gtatgtcatt
     5341 cttgcctcgc ggaaaggcat agaaaaaaaa tatggggaga ataccttcaa tgtcatattg
     5401 aatcttgtgc aggaactgac agctgcaatc aatcttcaca aaaattggag cgcattgtat
     5461 ttcctgccag atgacggaaa taacgtgctg caatatgggt tgacaaccat cgaaagcaat
     5521 gatccctggc agatcaaact ggcgctgcat gacctctcgg catacctgca cgatcatggc
     5581 aagaagatcg gtgcagtttt gatcatcgga ggagatgatg tggtaccttt tcatcatctg
     5641 cccaatccca ccgacgattc tgatagcgaa gttttgtctg ataaccctta tggcagcgat
     5701 gacaacaatt atttcattcc agagtgggcg gtcggtcgct ttccagacga aaaaggcagt
     5761 gatcccaatc tgttgttaaa acaattgcgc caaacgatcc aatggcacaa gaacaataca
     5821 aagaagagga gctttgcaga aaaagttgcc gattctttga aggtctggga ttcgattgct
     5881 ggttttttcc aggaaatcac agaaaccaag agcaattatg gatattccac agccatctgg
     5941 caacggtcgt ccatttcagt atttcgacct gttggaaagg caaatctgtt aagagtctca
     6001 ccaccttata atgccaacaa tcttgatgcg atcagcatga aagaagcgga atatgcgtat
     6061 ttcaacctgc acggtttgga tgattctccc aactggtatg ggcaaaagga tatttccaac
     6121 acagattcaa cgccagatta tccggttgcg ttgcaaccag gtttgattga tctggcggca
     6181 aagaacccga ccgtcgtagt ttcagaagcc tgctatggcg catatatcct tggaaagacg
     6241 aatgaagacg ccatcagttt gcggtttctt acgtcaggat gccgcgcttt tatcggctcc
     6301 atgtgtatcg cttacggttc ggtttatcct cctctgatcg gtgcggatca atttacttac
     6361 ctgttctgga atctgatcga aaagaattat tctgtgggag acgcgttcgt tagggcgaaa
     6421 ctcaatctgg tgaaatccat cttgcggaag caaggatatc tggatggaga agaccaaaag
     6481 acattgctct catttgtcct gtatggcgat ccgctgtatt acagtgattc gcaggcggaa
     6541 gatcatttga tccaggatca ggaaaagatt gtcgattcct atcaggtaat ccgcgatcaa
     6601 gacctggata ctgtagccgt tcccaaagtg tcctcggata tcttgatgaa cataaaggaa
     6661 gtggtaaaag aatatttacc ggggatcgag agcgctgagc tgagcattcg tgaaaagcaa
     6721 atgaaagtga tcaatatggt gcgcgatgca aaaggggcgg taaccatgga gaggcagact
     6781 gccggacggg tgtttctaac ctataagaaa agcttccaaa ttcaaaataa aacgctgaaa
     6841 caatttaccc gcgttacact ggatgaccgt ggaaagatga tcaagttctc cgtttcgaaa
     6901 taaaccttgc ctatttttaa ttgaattgtc cgttttcctc atttataatc aagagaatca
     6961 ctatcgaaaa tttttattta agagaccttt atgcaaaata aatcagacgg caacaccatc
     7021 cgcaagactt tcattgaatt tttcgaaaaa agagggcata cgtttgtccc ttcctcttca
     7081 ctggtaccgg ggggtgacca aacgctgctg ttcaccaatg ctggcatggt gcaattcaaa
     7141 gacgtctttt taggattgga tgaccgcgcg tatacccgag cagtaaactc acagaaatgc
     7201 atgcgtgtag ccggcaagca caatgacctg gaggatgtcg gtcgtgatga tatccaccac
     7261 acgtttttcg agatgctcgg gaactggtca tttggcgatt actacaagca agaagcgatc
     7321 acctgggcat gggaattgct tacagaagtt tggaagttgg ataaaaccac gttgtgggtt
     7381 acctgtttta aagatgaatc cggagagatc ccttcggatg acgaagccat tagcgcatgg
     7441 gaaatgcagc ccgggatcga tcaatctcac atactgcaat ttggaagaaa acaaaatttc
     7501 tgggaaatgg cagatatggg accatgcggt ccatgcagtg agatccatat tgaccgcggc
     7561 gaggaatttt gcaacaaaag aaacgatccc gaccatgtgt gcgcggtgaa tggaagctgt
     7621 acccgttttg tggagctctg gaaccttgtt tttatgcaat acaatcgcat cagccccaaa
     7681 aagttggttc cactgcctaa aaaacatgtg gacacgggaa tggggtttga gcggatcgta
     7741 tcggttttac agggtgtgga ctcgaattat aagacggatc tcttgctgcc gctcatggat
     7801 gtggtcttgc gattgacggg ggcaagcgta gaggaacgtg aaaagaacgt gaccccttat
     7861 cgagtgattg ccgaccatgc ccgggctgct actttcttga tcagcgatgg tgtggtgccg
     7921 gggaacattg gaagaaatta catatgtcgc atgattatcc gcagagcagc ccgcttcggt
     7981 acaaaactgg gactccatga gcctttcctt gccggggtcg cccatgaagt gatacgaatt
     8041 tatagagatg cctaccctga cctggtgaaa aaccaggaaa ccattctgga taatcttacc
     8101 cgcgaagaac aacgcttcaa aaaaacgttg gataccggtt tgtcgtattt gagtcagata
     8161 atggaagaga acaagaaaac gaaattgatc gctggcgaaa ctgcgtttga actgtatgcc
     8221 acccatggtt tgccattcga aattacgcgt gatatcgccg gagaagaggg atatttggtt
     8281 gatgaagaag cttatcgcga agccatggaa cggcatcggg tggaatcggg cgctggaaaa
     8341 gagttcggtc ccttaggtgg ggaagatgct gaaagatatg ccagtttctt caatgagatc
     8401 tatgatgcag ggtggctgcc caaagagggt gtcacatatg atccctatga tattttcccc
     8461 accgaacaac ccgtttctgc tctgatcaag gacgggaaat tggtggaatc ggtttctgaa
     8521 ggtgatactg ttgaggtggt tcttgcgaaa accaatttct acatacaatc cggtgggcag
     8581 gtggcggata aggggaagct cgtctccaaa gatgggaaat gggaaatttc tatcgaagat
     8641 gtcaaaaagc ccaccgcggg gttgatcgta catgccggaa aagttatcgc ggggagtccg
     8701 caaacgggag atgtggcggt tctggaggtt gatgccaggc gcaggcagga tatcatgcgc
     8761 aatcataccg caacgcattt gctgcacgcc gctttacgca aaatcctcgg cgtgcatgta
     8821 cgccaggcag gatcgttggt ggcgcccgac cggctgcgct tcgattttac ccatccagaa
     8881 gctttgacca gagaggaaat tcaaaaggtc caagattttg tgaatcaggc gattctggat
     8941 aacgaaccgc tccacaaaga agaaaaggac ctccagcagg ctattgaaga aggtgccatg
     9001 gctctctttg gtgagaaata tggggagcgg gttagaacag tggtcatcgg agagaatgca
     9061 gggacttcgt acgaattgtg cggcggtact cacgtggata ctaccgggga gataggaaat
     9121 ttcatcatcg tcagcgaagg tagcgtggct gcaggcattc gcagggtcga agcagtgacg
     9181 ggcagagagg cgtacgctct tattcaaaaa cggttactgc ggttggagaa tactgcggaa
     9241 aaattgcagg tgggcgtgga ccaactggaa aaaaagatag aaagcgttct cgaccacgaa
     9301 aagcagcttg aaaaagaaag ctccgcatta cagaaaaatg ctgtttttca aaaatatcgt
     9361 gatcagaaaa actcaggaga aatcattgga gcttcaagaa ttctctcact agcctttgga
     9421 ccaacaaacg gttccattct tcttgagttg tctgacctgt ttcgcaatga aaacaattct
     9481 ggaatcgcga ttttttcagg catcaatgag aaggacgagc ctttcttcat ggttaccgta
     9541 acggatgatt tagtgaagaa aggcatacat gcaggagaga tcgtaaatac ggcagcttcg
     9601 gtcgtggggg ggcgaggtgg gggaagacct aatttggcgc aggctggtgg gaaagatgcg
     9661 tcaaagatca atgaagcgct ccaaaaagcg aaagattatg ctcgaggcaa actggagtaa
     9721 gaaaaaccgc ccgcaaaggc ggttttcgat tcattcattc tgcgggaacc aacagcataa
     9781 aaccaggtct ttggctgctt ttacctcatc caatctcccg ataggcgtac catgcggcgc
     9841 cgaatgcaac aattcaggtt ccttatgagc ttcttcagcg atcttcttca tgatctccgc
     9901 gaaccgttca acctcctgct tcgattcggt ttcggttggc tcaatcatta atgcttcttt
     9961 gacgatcagc gggaagtagt tggttggggg atggacccca taatccatca tgcgtttggc
    10021 tatatccaaa gcatggatac cgggagcgtc ttcccaaaag ccttcaacga caagttcatg
    10081 catgcatttc ctatcgaatg gaacagtata gatgttctcg agcagttttt ttagataatt
    10141 tgcattgagc actgcatttt ctgaaatctt gcgcagtcca tctgatccat gcatggctat
    10201 gtaggtgaac gctcgtatga gaacgccaaa atgcccatag aatgatttca tctgtccaat
    10261 gctctgagcg ggacgaatga agccatacag tggaggtgtc ttttcgtccc cttcctctac
    10321 gataccaacg atgggggagg gcaagaaatc aaccagatgt ggcgctactg caaccggtcc
    10381 tgaaccgggg cctccgccgc catgaggggt ggaaaaggtt ttgtgaaggt taaaatgcat
    10441 gatgtcaaac ccgatctcgc ctggtttgat aatgccgagc agagcgttga aattggcgcc
    10501 gtccccatac acgagtccgc ccctctgatg gatgatatcg ataacctctt tgagatgttc
    10561 ttcaaaaaga cccaatgtgt tcggattggt gagcataagc cctgcaacac aatcgtcgca
    10621 aacttccttt aatttctcaa ggtcgatatt gccgcgacta tcagagggaa cgttcatcac
    10681 atcaaatcca ctcatcgatg atgtggcggg gtttgtgcca tgtgcggaat caggaaccag
    10741 gatcttggtt cgtttgagat cgttgcggct caaatgatag gctcggatga tcagaccgcc
    10801 cacaaattct ccctgtgcgc cggcagccgg ttgcaaggtg atacctgcaa acccgctgat
    10861 ctcttttaac cattcttgca gttgataaat gagtgccaga tttccctgga tcgtttcgat
    10921 gggttgaagc ggatgggtca tggcgaaacc cgggagtcga gccagctctt cattgatccg
    10981 cggattatat ttcatcgtgc aggagccgag aggataaaat ccactgtcaa tgctgtaatt
    11041 caagtgggaa aggtgcgtga aatgacggat gacgtcgact tcagatactt cagggaatgg
    11101 gaggtcatct cttttcaatt cggcaggtat cacgctaacc ggcacatctg gatcgtgaaa
    11161 ctggaatcct tttctagcgg atgaggagag ctcataaatg gtcggttcag ccatggttgt
    11221 tctccttcag aatatcacaa agaacgtcga tgatctcctt attggtcttt tcggtgaatg
    11281 ccagcagcat gcaatctttc agagcgggat atagattttc cagggcaaat ccacccagaa
    11341 taccttcttc cagaaggtga tggttgagat ggtcgacaga gctagggcat tggacgacta
    11401 tctcatgaaa gaatggttcg ggagtgagca ctttgaaccc ttccaacgct gctatctttt
    11461 tcgctgcgta atgcgcgttc tgataacaaa gattgctgac ctgctgcatt ccagatttcc
    11521 cgagcaaact catatatacg gttgcagcca atgccatcaa accttgattg gagcagatgt
    11581 tggaagtggc tttttcgcgg cgaatgtgtt gctcgcgtgc tgtcaacgtg agcacatatc
    11641 cgcgttgacc atgctcatca accgtttcac ccaccaaccg accggagagc ttgcgcacca
    11701 gttctttacg agctgcaatg aatcccagat aaggaccgcc aaaagaaagc gggattccca
    11761 aaggttgccc ctctccaaca gcgatatcag cccccatctc ccccggcgtt tttaataacc
    11821 ccagcgcgat agggttgact gccatgcaaa cgagagcgcc atggtgatga gcgatagtga
    11881 tgagctcggc aggatcaccg attttgccga agaaatcggg gtactgcaca atgaccagag
    11941 cagtatcttc atcgataaga tctttcatcg ccatcggatc agaaaaaggt tcgtcggggt
    12001 ttccagaaat cacgatgtcg atattctgaa taccgctgag gtatgttcga atcacctctc
    12061 tgtattgcgg atttagagcg tgcgagagaa tgatcttttt tcttttttcg cggaagtggg
    12121 agtaagcggt aatacaggct tcagccgcgg ccgttgcccc atcatactgt gatgcattgc
    12181 tcacttcaag agcggtgaga ttacagatca aagattggaa ttcaaaaatt gcctgcaagg
    12241 tgccttgaga aatctctgat tggtagggcg tataggctgt gaagaactct cccttgcgaa
    12301 gaatgctatc gaccgcagcc ggaatgtaat gatcgtaggc tcccgcgccc aggaaacaca
    12361 caaattcttt cgtgcttgcg ttggcgtctg caaagccttc gatatattcg cgagcaaaca
    12421 tttcactcat gcttgcaggc agatccaacg tcggaaagcg atatcgttcc ggaacgtcag
    12481 aaaacagctc ctcgatagat ttcatcccga gagctgttaa catttctttt cgttccgcat
    12541 cagtgtgcgg cgtgaacatt agtcgcctcg atcttcgcag aatctatcgt atatctccgc
    12601 atccatcaac gcatccactt cg
//
LOCUS       DAOSDS010000056        12501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1946_length_12501_cov_5.665997, whole genome shotgun sequence.
ACCESSION   DAOSDS010000056 DAOSDS010000000
VERSION     DAOSDS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12501
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1946_length_12501_cov_5.665997"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /gene="dnaA"
                     /locus_tag="PLL88_07480"
     CDS             <1..812
                     /gene="dnaA"
                     /locus_tag="PLL88_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOJ01444.1"
                     /translation="SEEFTNDLINAIRAHNTEDFRKKYRYMDVLLIDDIQFIAGKEST
                     QEEFFHTFNTLHSQNKQIVISSDRPPKAMTTLEERLRSRFEWGLTVDIQPPELETRIA
                     ILRAKAESANRSVAYEILEYIAQKIQWNIRELEGALTRVLAYSELSGRPLTMELTASA
                     LVDFFPDESPVAPDRIIRMVASAFNISEDDLLGKVRTKEIALPRQVAMYLMREESDAS
                     LPQIGNSLGGRDHTTVMYACDKIADMIERDDRFRRQVFQIRDKLHAEQSVL"
     gene            988..2631
                     /locus_tag="PLL88_07485"
     CDS             988..2631
                     /locus_tag="PLL88_07485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOJ01445.1"
                     /translation="MKTKSLKIIPIGGLGEVGRNMMVYEYDDDIIIVDCGLMFPNNDM
                     LGVDYIIPDFQYLRNNRHKIRGLIFTHGHEDHIGAVNYFLEEFNDVPVYATPLTMGLV
                     KVKLGKASLTQNVALHTVQAGSQIKIGKFKVDFFHVCHSIPDSVGLGIETPEGLIVQS
                     GDYKFDHTPVDNWPTDYAKLVEFSKRGVLALIADSTNSTRPGWTPSEQVVGEALDKVF
                     SQAKGRIIVASFASLVSRMQQVVNAAKKFNRKIAFVGTSMVENAKIAQKLGFLSIEDD
                     QKISIEEALHLQDGQVTIMCTGSQGEPTSILGRLSRGTQRQFDIKEGDTVVLSSKTIP
                     GNEEAVFSTINDLFRRGANVIYEQIAPVHVSGHGSQEDIKLLLHLTKPKYFIPAYGEL
                     RQLKQSAILAQQTGIPERHVIVVEDGQVITAKNNKLRLGDMEPISMVFVDGSGVGDIG
                     PDEMHDREMLSKDGVILVHVILNKKDGALITDPEIISRGFTSTREADELFPQARRVIL
                     DIIKRSPSSLDKEITKILSGFIYRETQRRPTILVTISKI"
     gene            complement(2628..3317)
                     /locus_tag="PLL88_07490"
     CDS             complement(2628..3317)
                     /locus_tag="PLL88_07490"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOJ01446.1"
                     /translation="MHALEDREAAFSEALAQMVEVQIHQRGITDPAVLAAFREVPRHR
                     FAPEEEWEEAYADHPISIGFGQTISQPYIVALMTEAARVKKGDHVLEIGTGSGYQAAI
                     LSRLGKTVDTVERIPALGAQAQRTLNDLGFENVRVHIADGTAGYVEASPYDVILVAAG
                     APSVPPPMQEQLKAGGRLVIPVGSSWHQELQLWTKTGDDFEMETILPVVFVPLLGQWG
                     WAEDRYRNDRS"
     gene            complement(3336..4418)
                     /gene="mutY"
                     /locus_tag="PLL88_07495"
     CDS             complement(3336..4418)
                     /gene="mutY"
                     /locus_tag="PLL88_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOJ01447.1"
                     /translation="MNVQTLSSSLIAWYQKNKRDLPWRETKDAYSVWVSEIMLQQTRV
                     ETVIPYYQNWMQKFPTLQTLAQASEEDVLLQWEGLGYYSRVRNLHHTAQILVQQNSGA
                     VPESIDELKKLPGIGDYTASAIASISFRAKVVALDANGKRIFARLLDVDMPVSSGQAK
                     KQILRFAADQIEQVDAGDFNQAIMDLGSMICLPRRPRCRICPIRNFCIAYQRDTQNIR
                     PVLEKKKAIPLYHVVAAVITDGDDRFLLAKRPRGGMLPGLWEFPGGKIEQGEDDQSAL
                     QREIREELDAAISVGELVGSYKHAYTHFRVIVRAYYCSVNGKLPRAVEAQELAWVKCE
                     DIAGFAMGRVDRWISHDICRGVSHEI"
     gene            complement(4430..4741)
                     /locus_tag="PLL88_07500"
     CDS             complement(4430..4741)
                     /locus_tag="PLL88_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01448.1"
                     /translation="METEPGKIILTWDVTPQYEQKYFEFLVREFLPGMQKLRFQLTDA
                     WVTVYGNRPQILVGAVMTSVEEIRTVLDSAEWFTLTGKLKEYVINFKQKITVANGGFQ
                     F"
     gene            complement(4741..5415)
                     /locus_tag="PLL88_07505"
     CDS             complement(4741..5415)
                     /locus_tag="PLL88_07505"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ01449.1"
                     /translation="MEIVWYGHSCFRITERGKASVVCDPFDSEVVGYSSLHLKAEIVT
                     ISHDSPGHNYTKCVKGNPYLITAPGEYEVGGVFITGLNTVRKVKKNGDADSNTAIAAD
                     NTLYLIDYSGISIAHLGDMSQVPSQAEVESLGPVNIALVPVGDGNSLNASKAAEVISL
                     LEPNIVIPMHYATADSKVKIDPIAKFLKEMGLTEIETQESYKISRIADLPEDTQVVIL
                     DYAMKS"
     gene            5502..5864
                     /locus_tag="PLL88_07510"
     CDS             5502..5864
                     /locus_tag="PLL88_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01450.1"
                     /translation="MPKKSRTIRSFQFKEALLPIVKKWAEDQRFTVEVDGNTITCEKG
                     IGLMTSPITVTLEKTKETVQFEAYLKVDAITMFSSLLMAPEEMHLESGDGELTMERKY
                     GRDKVNKLLEMLGQPLIE"
     gene            complement(5872..9633)
                     /locus_tag="PLL88_07515"
     CDS             complement(5872..9633)
                     /locus_tag="PLL88_07515"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ01451.1"
                     /translation="MNVPPQSGESWEWQDILDFSGKLLDEENIQSQVKLIKQHLSTRL
                     QAKVSIWLDNSFLPLPDETGTTIFKTDGCSPLMKRALASRQIVKKQDGAALSIALPLY
                     FQERVIGALEISDLAPAKISGQKAKLSAFAQQVSSGLFHTHQMRIKEWRSKQLELVRS
                     VSTKIIQQHDVHKIFRFVTDLVAETFHYYFVAIYMLDERREKLTFQASSGKEITRDPD
                     FQDIFANGIPLGKGLIGCCAAKKKEVLSNDVQDDERFHFVTGLPDTRAEVCFPLLAEK
                     QVIGVLDVQSNFLNGFHQNDLLVLQILADAIAGALHNARLLQNVRMQNERIQIVSEIT
                     RALSSILDLEKLLDEIVTIISHKFNYPYVHIFTIDRVMNRVVFRTGTGKSTEVLKANG
                     LWFDLESEKGIVAYAARKKKPYLSADVLDDDLYLPTDLPPHDPRSELAIPIIYSGEVL
                     GVLDIQSPQSDAFSEKDIDLFHTLSAGIAIALQNALIYRSERWRRKVAESFHEIANIF
                     SRDYSLEEIYKSVLKIASQNLPCTAGVIWQLTDATELTIADSIGVEKEDLSVSILEED
                     LEFIRYAIEQGEPLIRYGYDETEPLGRFLKLKKNYSAIVAPIINANQKLGAIEIVYEN
                     PNRYGEEARAVLKAFAGYTAVAIQNLGLFSASQEQALMATTLLLVSEATDQVETSEAL
                     FATIARLIQFVVGVQKTLMYKRDYSGQFMLAASIGISEGKEALYREYPTTFDGILTFS
                     RMLGEQVMEYQSVSISADETGEWLVIPMRIQDKVLGALFVQIDEGSADHDFLGGSGAK
                     QREVLSAVARQSAIALENLNLKRAEQTEAYVTAVLLQVAEAVVSSANIHETLENIVNI
                     LPLLVGTDCVMFLIWDKNRNIFQLADSFCGKWEEEIQNFDEYVSPHDYEPLELLIQQK
                     TPVYLAIDDLNPRLWFEQKRYKILADRDAVLEAKQDVQFLFPLQDREDFYGAMLVNDS
                     CEQFEYREKRVDILNGVAQQLALAFQNEHLREQMVGRERLQKEFQLARDIQKAFLPEI
                     VPQIPGWEIGVRWRPARQVGGDFYDFIWLPDGRLGFVIADVSDKGIPAALYMTVTRTL
                     IHATAKEELNPAQTLSKVNRLLVESSPQGLFITTFYGILDPRTGIFEYTNAGHNQPIL
                     INKKTDSVTLLEKGGMPLGVSDDFTIFNKTVEINHKETLILYTDGVTEAQSPDGKLYG
                     MNRFEKFMLESSITNMNDLLDALDKNILIFQKGFPPTDDLTVIALHRTK"
     gene            complement(9630..10049)
                     /locus_tag="PLL88_07520"
     CDS             complement(9630..10049)
                     /locus_tag="PLL88_07520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ01452.1"
                     /translation="MPLEIFPANFKNLEKIRNFYARAARAAGLDEDAVYDVQMAVDEA
                     AANIIDHAYGGEDQGVIECSYEITTNGIRIILHDHGKAFDPDLIAPPDISSDPCSRKP
                     RGLGLYFMQKLMDHVSFTFNGDGGNILTMEKNKEHCC"
     gene            complement(10058..10393)
                     /locus_tag="PLL88_07525"
     CDS             complement(10058..10393)
                     /locus_tag="PLL88_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOJ01453.1"
                     /translation="MEIRTTPLKRCTLLEVNGRIDSSTAPQLANVFNEITEGGEFHIV
                     FDMSNVEFISSAGLWVMVTAQKKCKRFNRGEVYLAGVPKRVYEALDLAGFIPYYKVFD
                     SAAEAVGSF"
     gene            10488..12005
                     /locus_tag="PLL88_07530"
     CDS             10488..12005
                     /locus_tag="PLL88_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01454.1"
                     /translation="MRKNIIIILILCVLSFIASAVVNTKGIKDAHMLSLLSADESIQY
                     PYLTHMLSGGNNLFETVKNFVSYQHYFYGYPFYVISAASILPVRLMSGTAFAEQVQLN
                     LLLLRQFVSVFPMLLVIFLLVYLQTGFRDGAKSVLLFFILITIPAVTRHNLWFWHPDA
                     LALLGIVGSLFFLNKDQFQLGKSFTYAAIACGLAAGIKVIGAFFFLAVLVYLWMAKKQ
                     ASLNMRQIVKKGAIFLLVFLLVFLITNPLLLIPQTRSQILKIQAQQNYFVREGWTDED
                     IYQTGLTAWLPYFREWYAHGLILILLIISILYGILQGKHRLMNTLLMAWIIPYSIYLI
                     FFVAVKPFHYPLPVMVPLFSAALNLLPEKQEVETRPGAKPLKITIVALAVFLVVTNGI
                     SSWKVYREYYEKEDLLLACNSAAENPADGSTVTIPGDGWYRLEEFDLTGEQKSHSFSA
                     VQGPLVVSASQQSGSQAWACRDEENAYFSASRLAEYFKKSYPDFVVIGPDGAEIK"
     gene            12156..12228
                     /locus_tag="PLL88_07535"
     tRNA            12156..12228
                     /locus_tag="PLL88_07535"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12189..12191,aa:Thr,seq:tgt)
     gene            12303..12385
                     /locus_tag="PLL88_07540"
     tRNA            12303..12385
                     /locus_tag="PLL88_07540"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12337..12339,aa:Tyr,seq:gta)
     gene            12428..12500
                     /locus_tag="PLL88_07545"
     tRNA            12428..12500
                     /locus_tag="PLL88_07545"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12461..12463,aa:Thr,seq:ggt)
BASE COUNT         3260 a         3171 c         2841 g         3229 t
ORIGIN      
        1 catcggaaga atttaccaat gacctgatca acgccattcg agcccacaac accgaggact
       61 ttcgtaaaaa atacaggtat atggatgtac tgttgatcga tgacattcaa ttcattgccg
      121 gcaaagaatc cacccaggaa gaatttttcc acaccttcaa cacgctgcat tcgcagaaca
      181 agcagatcgt gatttcatct gatagaccac ccaaagcaat gacgaccctg gaagaacgct
      241 tgcgttcgcg cttcgaatgg gggctcaccg tcgacatcca accccccgaa cttgaaacgc
      301 gtatcgcgat cctgcgcgcc aaagccgaaa gcgccaaccg cagcgtagcg tacgaaattc
      361 tggaatacat cgcacagaag atccaatgga acatacgcga attggaaggt gccctaacca
      421 gggtgctggc atactccgaa ctcagcggaa gaccgctgac catggaatta accgcttcgg
      481 cattggtcga tttttttcct gatgagagcc cggttgcccc cgaccgcatc atccgcatgg
      541 ttgcgagcgc attcaatatt tcagaggatg acctattggg aaaagtccgc accaaagaaa
      601 tagccctgcc aaggcaggtt gccatgtacc tgatgcgcga agaatctgat gcatccctgc
      661 cgcagatcgg caacagtctg ggcggtcgcg atcacactac ggtaatgtat gcttgcgata
      721 agatcgccga tatgatcgaa agagatgacc ggttcagacg tcaggttttt caaattcgtg
      781 ataaattgca cgctgaacaa tccgtccttt aaaatagcgc tgtatttatt gaaaaacgtg
      841 ttagaatgtg tatccggtaa ttcatggatt actgggtaat tatcgaaaat tgaaataaca
      901 attgagaaag aaagccctgg ccgccatttg aaccagggca ttttatttaa ttaaaccaat
      961 aaccttaaaa ggtttatatg aggaaatatg aaaacaaaat ctttaaaaat catccccatc
     1021 gggggactcg gagaagttgg gcgcaatatg atggtctacg aatatgacga cgatatcatc
     1081 atcgtcgatt gcggtttgat gttccccaat aatgacatgc tcggggtaga ttacatcatc
     1141 ccagattttc aatatctgcg caacaatcgc cacaagatcc gcgggttgat ctttacccac
     1201 gggcacgaag atcacatcgg ggcagtcaat tactttttag aagagttcaa tgacgttccc
     1261 gtgtatgcca ctcccctgac catggggctg gtaaaagtaa aactcggaaa agcgtcgctc
     1321 acgcaaaacg ttgctctgca cactgttcag gctggcagcc agattaaaat cggcaaattc
     1381 aaggtcgatt ttttccacgt atgccattcc attccagatt ccgtgggatt gggcatagaa
     1441 acacccgaag gcttgatcgt ccaatcgggt gattataaat ttgaccatac cccagtggat
     1501 aattggccaa cagattatgc caaactggtt gaattttcca aacgaggtgt gctggcgctg
     1561 atcgctgatt caaccaattc aacccgacca gggtggacac cctcggagca ggttgtagga
     1621 gaagcgcttg ataaagtgtt ctcacaggca aaagggcgca ttatcgtagc cagttttgct
     1681 tctctcgtat cacgcatgca acaagttgtg aacgctgcga aaaaattcaa tcgcaagatc
     1741 gcctttgttg gaaccagcat ggtcgagaac gccaagatcg cgcaaaaact cggcttttta
     1801 tccatcgagg acgatcaaaa gatcagcatt gaagaagcgc ttcatctgca ggatgggcag
     1861 gtgaccatca tgtgcaccgg atcacaggga gagcccactt ccattcttgg caggctatcc
     1921 cgtggcacac aacgccagtt tgacatcaaa gaaggcgata cggtagtgct ttcttccaaa
     1981 accattcccg gcaacgaaga ggcggtcttt tctacaatca atgacctctt ccggcgtggc
     2041 gcgaatgtga tctatgagca gatcgctccg gtacatgttt cagggcacgg cagccaggaa
     2101 gatatcaagt tgctgctgca tctgaccaaa ccaaaatact tcatccccgc ctatggtgaa
     2161 ttgcgccagt taaagcaaag tgccatcctg gctcagcaaa ccggcatccc agaaaggcat
     2221 gtcatcgtag tggaagatgg gcaggtcatc acggctaaaa acaacaaact ccgcctgggc
     2281 gacatggaac cgatctctat ggtgttcgtg gacggctcag gggtggggga tatcggtcca
     2341 gatgagatgc acgatcgcga aatgctttca aaagatggcg tgattttggt gcatgtcatc
     2401 ttgaacaaaa aagacggggc tttgatcacc gatccagaga tcatcagccg cgggttcaca
     2461 tccacccgcg aagcggatga acttttcccg caggcacgca gagtcatcct ggatatcatt
     2521 aaacgaagtc cttccagcct ggataaagag atcaccaaga ttttgagcgg tttcatctac
     2581 cgcgaaacgc aacgccgccc gaccattctg gtgaccatca gcaagatcta gcttctatcg
     2641 ttccggtaac ggtcttcagc ccagccccat tgccccaaca ggggcacaaa gactaccggg
     2701 aggatcgttt ccatttcaaa atcgtccccc gttttcgtcc acagttgcag ctcctgatgc
     2761 caggagctgc ccaccgggat gaccagccgt ccacctgctt ttaactgctc ctgcatggga
     2821 ggtggtacag aaggcgctcc ggcagcgacc aggatgacat catacggaga agcttcgaca
     2881 tatcctgccg ttccgtctgc aatatgaact cgcacatttt caaatcccag gtcattcagt
     2941 gttctctggg cttgtgcgcc cagtgccggg atgcgttcaa ccgtatcgac cgttttgccc
     3001 aatctggata agattgccgc ctgataaccg gaacccgttc caatttcaag aacatggtct
     3061 ccctttttca cccgtgcagc ttccgtcatt aacgccacaa tatagggctg cgagatggtt
     3121 tgtccaaaac caatggaaat ggggtgatca gcataggctt cctcccattc ttcttcaggc
     3181 gcaaagcgat ggcgcggcac ctcacgaaaa gcagccagca cggctggatc ggtgattcca
     3241 cgctggtgaa tctgaacctc caccatctga gcgagagcct ccgagaatgc ggcttcacga
     3301 tcttccaggg catgcataaa cctattataa gataatcaaa tctcgtgtga aacgcctctg
     3361 caaatgtcat gcgatatcca gcggtcaacc ctgcccatag caaagcctgc tatatcttcg
     3421 catttcaccc atgccagctc ctgcgcttct acagcgcggg gcagctttcc attcacgctg
     3481 caatagtaag cccgaacaat aacccgaaag tgggtataag catgcttata acttccaacc
     3541 aactcaccca cagagattgc agcgtcaagc tcttcgcgaa tttcgcgctg caaagccgat
     3601 tggtcatctt ctccttgctc aatttttccg cccggaaatt cccaaagccc gggcagcatg
     3661 ccaccccgcg gtctctttgc caacaaaaag cggtcatcgc catcggtgat caccgcagcc
     3721 acaacgtgat aaagcgggat cgcttttttc ttttccagaa ccggacggat attttgggta
     3781 tctcgttgat aagcaatgca aaagttacgg atcgggcaaa tgcggcagcg gggacgccgg
     3841 gggaggcata tcatggaacc cagatccata atagcctggt tgaaatctcc ggcatcaacc
     3901 tgctctattt gatcagcagc aaaacgtaaa atttgttttt ttgcttgacc actggaaacc
     3961 ggcatatcca catccagcaa tcgggcaaaa attcgttttc catttgcatc caaagccacc
     4021 accttagcgc ggaagctgat cgaagcaatt gcactggcgg tatagtcgcc gatgccgggc
     4081 aattttttaa gttcatcgat cgattccgga acagcgcctg agttttgctg taccagaatc
     4141 tgcgccgtgt ggtgcagatt gcgtacgcgc gaataatatc ccaaaccttc ccactgcagt
     4201 agcacatcct cttcagacgc ctgtgccagg gtctgtagtg ttgggaattt ctgcatccag
     4261 ttttgataat agggaatcac cgtttcaaca cgcgtctgct gcagcatgat ctcagacacc
     4321 caaacagaat aagcgtcttt tgtttcgcgc cagggcagat cccgtttgtt tttctgatac
     4381 cacgctatta agctgctgga aagggtttgc acattcacaa agagaatttc taaaattgaa
     4441 agccgccgtt ggcaactgtt attttctgtt taaagttgat cacatactct tttagttttc
     4501 cggtaagggt aaaccattcg gcactatcca atacggtacg tatctcttcc acagaggtca
     4561 tgacggcgcc cacaagtatt tgcggacgat ttccatagac ggtcacccag gcatccgtca
     4621 attgaaaccg aagtttttgc atgcccggca agaactcccg tacaagaaat tcgaaatatt
     4681 tctgttcgta ctggggggtc acatcccacg tgagaatgat ctttcctggt tcggtttcca
     4741 ttatgatttc atcgcgtaat ccaatatcac aacctgggta tcttccggca ggtcagcgat
     4801 gcggcttatt ttatagctct cctgggtttc aatttcggtc aatcccattt ctttcaagaa
     4861 ctttgcaatg ggatcaattt tcactttcga atcggctgta gcataatgca tggggatgac
     4921 aatatttggc tccaacaaac tgatcacctc tgcagcctta ctggcattca aactatttcc
     4981 atccccaacc ggcaccagcg cgatattgac cggtcccagg gattctactt cagcttgcga
     5041 cggaacttga gacatatctc ccaggtgagc aatggagatg ccgctataat cgatcaaata
     5101 caaagtattg tctgcggcaa tggcagtgtt ggaatctgca tcgccatttt tcttcacttt
     5161 gcggacggtg ttcagaccgg tgataaaaac accgccgact tcatattccc caggtgctgt
     5221 gatcaggtac ggattccctt tcacgcattt cgtataattg tgccccgggg aatcatggct
     5281 gatcgtgaca atttcggctt tcagatggag agagctatac ccaaccacct cactgtcaaa
     5341 cggatcacag accaccgacg ctttaccccg ttccgtgatg cgaaaacaag aatgcccata
     5401 ccagacaatt tccataacac ctccagaatc attttgatta tactttagat atgtgcacac
     5461 ccttaccaat cataaaagaa ttttagagtt ttgaggtaaa catgccgaaa aaaagccgca
     5521 ctattcgatc tttccaattc aaagaagctc tcctgcccat cgttaagaaa tgggcagaag
     5581 atcagaggtt cacggttgaa gtggatggca acacaatcac ctgcgaaaag gggatcgggt
     5641 tgatgacctc tcctataacg gttacgttgg aaaaaacaaa ggaaacggtg cagttcgaag
     5701 catatctcaa agtggatgcc attacgatgt tttcatcctt gttgatggct cctgaagaaa
     5761 tgcacctcga atcgggcgat ggagaactca ccatggagag gaaatatggg cgcgataagg
     5821 tcaataagct gcttgaaatg ctcgggcagc cactgatcga atagggctcc tttactttgt
     5881 gcgatgcagc gcgatgaccg ttagatcatc ggttggtgga aaacctttct gaaagatcaa
     5941 gatgttttta tccagcgcat ccaacagatc gttcatgttg gttatactgc tttccagcat
     6001 gaatttttca aaacggttca tcccatacaa ctttccgtct ggtgactgcg cttctgtgac
     6061 accatctgta tagagaatca gggtctcttt gtggttaatt tctactgttt tattgaatat
     6121 cgtgaaatca tcggaaacgc ccagaggcat gcctcccttt tccagtaggg taacgctatc
     6181 ggttttctta ttaatcaaga tcggctgatt atgcccggca ttggtgtatt caaaaatacc
     6241 cgtcctggga tccagaatgc cataaaaagt ggtaataaac agaccctggg gggagctctc
     6301 caccaataaa cggttcacct tggaaagcgt ctgggctggg ttgagttctt ctttggcagt
     6361 tgcatggatc agggttcttg tgaccgtcat gtacagcgca gccgggatgc ctttatcaga
     6421 gacatcggca atcacaaatc ccagtctgcc atcaggaagc catataaaat cataaaagtc
     6481 acctccaacc tgccgggcag gtctccaacg cactccgatt tcccaacctg gaatttgagg
     6541 gacaatttcc ggcaaaaaag ctttttgaat atcacgagca agttgaaatt ctttctgcaa
     6601 cctttcacga ccgaccatct gttctcttag atgctcgttc tgaaaagcca gggcaagctg
     6661 ttgggctacc ccattcaaaa tatcaacccg tttttctcga tattcaaact gctcgcatga
     6721 atcattcacc aacattgctc cgtagaagtc ttcgcgatct tgcaatggaa acaggaactg
     6781 cacatcttgt ttcgcctcaa gaactgcatc gcgatccgcc aggattttat agcgtttctg
     6841 ctcgaaccac aaacgtggat tgaggtcatc gatagcaagg taaacagggg tcttttgctg
     6901 gatcagcaat tccaaaggtt cataatcatg cggcgaaaca tattcatcaa aattctgtat
     6961 ttcttcttcc cattttccac agaaagaatc agccagttga aagatgtttc tgttcttgtc
     7021 ccatatcaag aacatcacac aatccgtacc caccaggaga ggcaggatgt tcacgatatt
     7081 ttcaagtgtc tcatggatgt tcgctgagct aaccaccgct tctgctactt gcaacaaaac
     7141 cgccgttacg tacgcttcgg tttgttcggc acgtttcagg ttgaggtttt ccaaagcgat
     7201 ggctgattga cgcgccaccg ccgaaagcac ctcgcgctgt tttgcacccg acccgcccag
     7261 aaaatcatgg tcagccgatc cttcgtctat ctggacaaag agagcgccca gcaccttatc
     7321 ctgtattctc atcggaataa ccagccattc ccctgtttca tccgcagaaa tggaaactga
     7381 ttggtattcc atgacttgct cgcccaacat tcgcgagaag gtaagaatgc cgtcaaatgt
     7441 ggtagggtat tctcgatata gcgcttcttt tccctcactg atcccaatgc tggctgccag
     7501 catgaattgc ccgctgtaat cgcgcttgta catcagtgtt ttctgcactc ccaccacaaa
     7561 ttggatgaga cgtgcaatcg tcgcaaacag cgcttcactg gtttcaacct ggtcggtggc
     7621 ttcactgacc agcaataaag tggttgccat taacgcctgc tcttgagaag cggagaacag
     7681 cccgagattt tggatcgcca cagcggtgta accggcaaaa gcttttaata ccgcacgcgc
     7741 ctcttctccg taccgattcg ggttttcata aacgatttca atggcaccca gtttttgatt
     7801 ggcattaatg atgggagcaa cgatcgcaga ataattcttt ttcaatttca agaatcttcc
     7861 cagcggttca gtctcatcat agccatagcg gataagcggt tcaccctgtt cgatagcgta
     7921 tcgtatgaac tccagatctt cttccaggat ggagacactc agatcttctt tttcaacgcc
     7981 aatcgaatct gcaatcgtca attctgtggc atcggtaagc tgccagatca ccccggcggt
     8041 gcaaggaaga ttctggctgg cgattttcag aaccgactta tagatctcct cgagcgaata
     8101 atcgcgcgaa aagatgttgg cgatctcatg aaagctttcc gcaacctttc tgcgccaacg
     8161 ttcagagcga taaatgagag cgttttgcaa cgcgatggca atgccggctg acaaggtgtg
     8221 aaacaaatcg atgtcttttt ctgaaaaggc atcgctctgc ggactttgta tatccaacac
     8281 accgagaacc tcgccagaat agataatggg aatggcaagc tctgaacgcg gatcgtgggg
     8341 tggtagatcg gttggcaagt aaaggtcatc gtccaacaca tcggctgaaa gatagggttt
     8401 tttcttgcga gccgcgtagg caacgatgcc tttttcactt tccaggtcga accataatcc
     8461 gttggctttt aacacctcag tgctttttcc ggttcctgtc cggaatacca cacggttcat
     8521 aacccggtcg atcgtgaaga tatggacata cggataattg aacttgtgac tgatgatcgt
     8581 tacgatctca tccaataact tttcaagatc aagaatagac gaaagggcgc gggtgatctc
     8641 tgaaacaatc tggattcgct cattctgcat gcgcacattt tgcagcaagc gtgcattatg
     8701 gagcgctcca gcaatcgcat cagccaggat ttgcaacacc agcaaatcgt tctggtgaaa
     8761 cccgttgagg aaattgctct gcacatccaa gacaccaatg acctgtttct ccgcaagcaa
     8821 tggaaaacaa acttctgccc tggtgtcagg caatccggtt acaaagtgga aacgttcatc
     8881 gtcctgcacg tcattgctga gcacttcttt cttttttgca gcacaacatc cgatcaatcc
     8941 ttttcccagt ggaatgccat tggcaaagat atcctggaaa tcaggatcgc gagtgatctc
     9001 ttttccggag ctggcttgaa aggtcaactt ctctcttctt tcatccagca tatagatggc
     9061 tacaaaataa taatggaatg tttctgctac caaatcggtg acgaagcgga atattttatg
     9121 aacatcatgc tgttggatga ttttcgttga aacagaacgc accagttcca attgcttgct
     9181 acgccattcc ttgatgcgca tttggtgggt atggaacaat ccgctggaga cctgctgggc
     9241 aaatgccgat agctttgctt tttgtccgct gatcttagcg ggcgcgagat cacttatttc
     9301 cagggcgcct atcacgcgct cctggaaata taacggcaag gcaatggaca gagccgcacc
     9361 atcctgcttt tttacgattt ggcggctggc aagagccctt ttcattaaag gggaacaccc
     9421 atccgtcttg aaaatggtcg tgccagtttc atcgggaaga gggagaaaag aattatccag
     9481 ccagatggag acctttgcct gtaagcgggt ggaaaggtgt tgtttgatta atttaacttg
     9541 cgattgaata ttttcttcat ccagcagttt cccactaaaa tccaggatat cttgccactc
     9601 ccaagattct ccagactggg gaggtacatt caacagcagt gctccttgtt tttttccatg
     9661 gtgaggatgt ttccaccatc accgttgaat gtaaaactga cgtgatccat cagtttttgc
     9721 ataaaataca gccccaaccc acgcggtttt cgcgaacagg gatcagagga aatatccggc
     9781 ggggcaatca gatcaggatc gaaagctttc ccgtgatcat gaagaatgat gcgaattccg
     9841 tttgtggtga tttcatagga acattcgatt acgccctgat cttcaccgcc ataggcatgg
     9901 tcgatgatgt tggcagccgc ttcatccacc gccatctgca catcatacac cgcatcttca
     9961 tccagccctg ccgcgcgcgc agcccgggca taaaagttcc ggatcttctc aaggtttttg
    10021 aaatttgccg gaaatatttc taacggcata tcaaagacta gaagctccct acagcttcag
    10081 cggctgaatc gaagactttg taatagggga tgaatccagc cagatccagc gcctcataca
    10141 ctcttttcgg cacacctgcc agatacactt caccgcgatt gaaacgcttg catttctttt
    10201 gagcggtgac catcacccag agaccggcac tggagatgaa ttccacattg ctcatgtcaa
    10261 aaacaatatg gaattctccc ccttccgtga tctcattgaa cacgtttgcc aattgaggag
    10321 ctgtagaact atcgatccgt ccgttcactt ccaacagcgt acagcgcttc aggggagttg
    10381 ttctaatttc cattattcct ccagtaattc ccaacaaaat ggtcataaaa aattatatca
    10441 ttcacaatag gagttatatt gtatagtagc atgaaagggt tcacaagatg agaaaaaaca
    10501 tcatcatcat tctaatctta tgcgttttgt catttattgc cagcgcagtt gtcaacacca
    10561 aagggatcaa agacgcccac atgctctcgc tactatcagc agatgagagc atccagtacc
    10621 catatctgac ccacatgctc agcggaggga acaatctttt cgaaacagta aagaattttg
    10681 tttcttacca gcattatttt tacggttatc cattctacgt aatttctgca gcatccatcc
    10741 tgccggtgcg gttgatgagt ggtacagctt ttgctgaaca ggtacagctc aatctcctct
    10801 tgctgcgcca gtttgtcagc gtgtttccta tgctcctggt cattttcctg ctggtctatc
    10861 tgcaaaccgg ctttcgagat ggggcgaaaa gcgttctcct cttttttatc ctcattacca
    10921 tacctgcggt cacacggcat aacctctggt tctggcaccc tgatgcgctt gccttgcttg
    10981 gaatcgttgg atccttgttt ttccttaata aagaccaatt ccagctcggc aaatcgttca
    11041 cctacgccgc catcgcctgc ggtcttgcgg cgggcatcaa agtgatcggg gctttcttct
    11101 ttttggctgt actggtctac ctgtggatgg caaaaaaaca ggcttctttg aacatgcgcc
    11161 agatcgtaaa aaaaggagcc atctttctcc tcgtttttct tctcgttttc ctcatcacaa
    11221 atcccttgct gctgattccc caaacccgaa gccagatcct caagatccaa gctcagcaaa
    11281 attatttcgt gcgcgaagga tggaccgacg aagacatcta tcaaaccggt ctcaccgcct
    11341 ggctgccata ttttcgagaa tggtatgcgc atgggctcat cttgatcttg ttaatcattt
    11401 ccattctgta tggaatattg cagggaaaac accgcctgat gaacacgcta ttgatggcat
    11461 ggatcatccc ctattccatc tatctgatat tctttgtggc tgtaaaaccc ttccattacc
    11521 ctttgccggt catggttcct ttattttcag ccgcgctcaa tttgctgccc gaaaaacaag
    11581 aagttgaaac ccgacccgga gcaaagccat taaaaatcac cattgtcgcc ctggcagtgt
    11641 ttctggtggt cactaacgga atttcttcat ggaaggtcta tcgggaatac tatgaaaagg
    11701 aagatttgtt gctggcatgc aattcggctg ccgagaaccc tgctgatggc agcaccgtca
    11761 ccatccccgg tgacggctgg taccggctgg aggaattcga tctcacaggg gagcaaaaga
    11821 gtcatagttt ctcggcggtg caaggaccgc tggtggtcag cgccagccag caaagcggca
    11881 gccaggcatg ggcatgccgc gatgaggaaa acgcctattt ttccgcttcg cggctggctg
    11941 aatatttcaa aaaaagctat cccgatttcg ttgtgatcgg tccggatgga gcagaaatca
    12001 agtaggattc tcaatagcag ggtaatttcc ttttaaaatt ccaaagtttt taagcttgtg
    12061 gtacaatcca gtccttgcct gctattttgg gttttggtat aatctcatgg ctgtacctta
    12121 ataaaaagtt agattggatt taatgatttt cgattgccga cgtagctcaa gcggcagagc
    12181 aaccgccttg taagtggtag gttacgggtt cgattcccgt cgtcggctca gttgaccgtt
    12241 cacaagtgat gcagatccgc tgcaagaact gctcacttgt gttcaggcaa cccaatcgat
    12301 atgggcggtt acccaagtgg ccaaaggggg ctgactgtaa atcagctggc agaagccttc
    12361 ggaggttcga atccttcacc gcccacaggt caaatgacca aagccgaatt tcggcgaagc
    12421 acggcaagcc ctcatagctc agtcggtaga gcacgttctt ggtaagaacg gggtcatggg
    12481 ttcaaatccc attgagggct c
//
LOCUS       DAOSDS010000057        12467 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1954_length_12467_cov_5.087013, whole genome shotgun sequence.
ACCESSION   DAOSDS010000057 DAOSDS010000000
VERSION     DAOSDS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12467
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1954_length_12467_cov_5.087013"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLL88_07550"
     CDS             <1..550
                     /locus_tag="PLL88_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01455.1"
                     /translation="QHGNTLTLFKANGLEYSSSSNFIEGKLAGEVLVTSEIKGTNLRL
                     QNNTLTQTSVCFVGFQNGERSLGVMLPIKRLLNERRVSEPFEGSFLIQANGAEFGMEW
                     LTYRYSTSSKKSCILYRIIQNPYPCNQDCAAIETEPGCECFCANFLYYDENCIPTEIN
                     PNCKCVCRGEGARFTPNGDDSP"
     gene            complement(522..2156)
                     /locus_tag="PLL88_07555"
     CDS             complement(522..2156)
                     /locus_tag="PLL88_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOJ01456.1"
                     /translation="MKISIWQGGMWHALLVVVLILLSGLLVYYTGGIKYVYSHFMYIP
                     IFLAAYFFDVPGGVIAGILGGLVLGPSMPMVVETGEMQTLYNWLYRLIIFTLVGGMQG
                     AVFATVKRQVKELHWQASHNRYTGLPNRTHLYLDLEQKKENRSLDTVYAIFMIQVDNH
                     ARITRILDMEELNKLNMMVFHRLKNILGDSSVLYQILPYLLCLAVEIDDERNTCDFIA
                     ESIYAALREPFEINDVPVFVNISIGVAQDNLKNMSPHISLQNATVAAQVASEKELKYW
                     IYEREEFQTARNSQALLGDVLQGIDRHEFKLNFQPIVRLQNGEVMGVEALLRWNHGKL
                     GLIPPMSFLPALEHTSLLYSLHDWEICEIVAQLASWKNFDGYLGINLSTRVLLDHSWI
                     ERFESLLDTFDLYASRIIFEITETAIIKDVERSLSALKRLRAIGSRIALDDFGTGYSS
                     LEYIQLLPVDYMKIDKSFVLGVHKEPKKQQIVHTALSLAASIEMQTIAEGIESQADFD
                     WLLKAGCMYGQGFWIGKPMPGNKLESWIKANHPHSA"
     gene            complement(2418..3053)
                     /locus_tag="PLL88_07560"
     CDS             complement(2418..3053)
                     /locus_tag="PLL88_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ01457.1"
                     /translation="MLKRLQRAMWKVVDLVNSPRKTQKITRSAVASRVIFERYLAAAR
                     VVDAKRFCAGSLAEMSMRVGGGKLMINAPDIPFGLLDVEALLFTGIEQQPHDQELMLP
                     KHTAWHQQIYRYSAATTVLLCQPHFLMVLTNRNQLPPKGILSDADALIERVKLIPASY
                     IDGEWVEDSNEIWLVPSVGVLSAGQTLEQNIDRVEILESVCAMAVLKSLIA"
     gene            complement(3076..4383)
                     /locus_tag="PLL88_07565"
     CDS             complement(3076..4383)
                     /locus_tag="PLL88_07565"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HOJ01458.1"
                     /translation="MSFKKLLYKFEKENQRPVRVGLLGAGQMGTGLISQIEKMYGIKV
                     VAVADVRKDRAYEAYQEASVDKTLLVKNDDDPEKAAQAIESGKRVACHSSDMIVQIPN
                     LDVVVESTGIPEVGAKFCYDAILARKSVVNMNVETDVVVGYQLAKMAEAAGVVYTLVA
                     GDEPGSVKELYDFADTLGFEIVCIGKGKNNPLHPDATPDTVAESAKAQNMNPKMLAAF
                     EDGTKTMAEMTAMANGTGYVPEVRGGYGPTVEVADLPKVFCPKKDGGILETRHAVDYA
                     MGKIAPGVFVIITTDQPKIIADLNYLHVSHHGNYWALYRPYHMANLETPISILRAFFY
                     HEETLATHSAPTAETITMAKRDLKAGEKVDYLGGYTVYGNVEKAEVARKEGLVPLGLT
                     VGATIIRDVKKGQPLHYAELELNESQTIYHLRWLQDKTLGYVE"
     gene            complement(4401..4763)
                     /locus_tag="PLL88_07570"
     CDS             complement(4401..4763)
                     /locus_tag="PLL88_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HOJ01459.1"
                     /translation="MIKFQGKVVSIGPLVDEFRQAGILVFFGEKAPEELVEFSIIHDA
                     KELIKPVESGDTVYIGAEGFTVLSVGDVANTNLANLGHLILKFSGEDTARLPGDVNLE
                     EKQIPAIAIGTEIKIEGN"
     gene            complement(4855..5235)
                     /locus_tag="PLL88_07575"
     CDS             complement(4855..5235)
                     /locus_tag="PLL88_07575"
                     /inference="COORDINATES: protein motif:HMM:NF015569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01460.1"
                     /translation="MADKKYKKIKIKAGPKGWGGPLTIEPTDEKNLIYSVTGGGIHPL
                     AAKIAELTGGTPFDGFKSRAPFEQIAVCVIDCGGTARVGVYPMKKVPTVDIYPTSPSG
                     PLFRFITEDVFVSGVRVEDIEVLD"
     gene            complement(5343..5876)
                     /locus_tag="PLL88_07580"
     CDS             complement(5343..5876)
                     /locus_tag="PLL88_07580"
                     /inference="COORDINATES: protein motif:HMM:NF019286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01461.1"
                     /translation="MAKFLEKIGRAVGKVVGILYQAGRESIDQVIKNVLPFMAFISMI
                     IGVILATGVGNFLANTLAPLSSSLIGLLIMTFIIGFPVLSPLLGPGAVIAQIIGTLLG
                     TLIGEGSIPPQMALPALFAINPQVGCDFIPVGLSLGEAEPETVEIGVPAVLFSRFVTG
                     PVAVVLAWLASFGLYSA"
     gene            complement(5886..6428)
                     /locus_tag="PLL88_07585"
     CDS             complement(5886..6428)
                     /locus_tag="PLL88_07585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HOJ01462.1"
                     /translation="MEGLAKFAEGFIGVFQAGGEQFVGLVTGIIPLLVVLMTAVNALI
                     ALIGPEKVENLGKVAARPGILYYPLRYMILPFVAVFFFTNPMAYTMGRFLPERYKPAF
                     YDAAVSFVHPPLGLFPHVNPGEYFVWGGIAAGITALGLPIGDLAIRYLLVGLVVILIR
                     GIVTEVITAIMLGRKAAEKK"
     gene            complement(6514..6960)
                     /locus_tag="PLL88_07590"
     CDS             complement(6514..6960)
                     /locus_tag="PLL88_07590"
                     /inference="COORDINATES: protein motif:HMM:NF018611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HOJ01463.1"
                     /translation="MQLGSAIFLVFIVAWVIQFGMTFFQLRRYNKRVAELKKMGPTAV
                     GMSGSMYKRRAYGVLVFDDQEKIVRAEQFSGWTVFASLKPVKELEGLTLQDIKDESVE
                     LPISKKIRSAFINAVDQVEYARKKAAEAAAQAQNDEQLDENSSKVV"
     gene            complement(6980..7933)
                     /locus_tag="PLL88_07595"
     CDS             complement(6980..7933)
                     /locus_tag="PLL88_07595"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HOJ01464.1"
                     /translation="MATDPITQKLMYKIARAYYDDGLTQQEIGSKFGLSRIKVGRLLE
                     KARREKLVQITIQEPKINADVELENRIEKKYHVDEVILVPARTDHQQTLQAIGQATAD
                     FLDRVISGDEIIGITWGGTMLAMVDALHSNNWPAMRIVQLLGGLGSSQADMYAADLTM
                     RMAQTFGAKARLLSAPGIVPTKEICDALRKEQQIEETLELGRRADIGIVGLGRPTSSS
                     PVIKYGILSPNELVELEKQGAVGDVGLRFMDANGKEIQNEINDRIIGLDFEQYKSIKK
                     LVGIAGGPEKFEAVKASLIGNLLSVLITDSEIGRRLVEQQE"
     gene            8129..9175
                     /locus_tag="PLL88_07600"
     CDS             8129..9175
                     /locus_tag="PLL88_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ01465.1"
                     /translation="MNDMNAVIYKGPGDYTLEKVPVPFISDKEVLLKIDAVGLCGSDL
                     RTLKFGHRKVTPPYILGHEISGTIRAAGALVDPQWAVGMRVALAPVVYCGNCHFCQRG
                     QFELCQHYREIGQSWSGGFADYLNIPQDALQHGVIHPIPASMDAVHATLAEPLAACLH
                     AIDLVDLRNVYTAAIFGSGAIGCLLLQLLRNQGLRSIIIIDPNESRLRIAEHFRPDHS
                     INANSEKAVEKICSLTGGLGVDLIFTATAAPVVQQQALDSISKGGQIVVFAGLPKGQS
                     VIPFDLNKVHYDCIRLIGSSIYAPHHHEEALRLIAEQKIDVKRIITTFPLAKFHEGVQ
                     AAISGEIVKAVFIP"
     gene            complement(9480..9728)
                     /locus_tag="PLL88_07605"
     CDS             complement(9480..9728)
                     /locus_tag="PLL88_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01466.1"
                     /translation="MSTCVISSFVRSIFPSLIIGVLSAFMWGPLSGVGRDKDPDNNWN
                     LRKKRYGSAFAAWFRLFLTFALIAYVIFTVALALKCYY"
     gene            9987..11045
                     /locus_tag="PLL88_07610"
     CDS             9987..11045
                     /locus_tag="PLL88_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOJ01467.1"
                     /translation="MGDQFPSFKIAAVQAAPVFLDREATTEKACQLIREAAAKGARIV
                     GFPEGFIPAHPIWFHFHPSTDKISTQLSVALFKNSVEIPGPQIAQLCQVAKETKTYVV
                     MGVCERNPGTMGTMYNTQVFIGADGSVLGKHQKIMPTTGEKLVHTGGYGDTLRTFPTE
                     FGPISGLICGENTNPLAIFALIAEFTRIHIASWPSNFTLISHPMRERMSFTAKAFAEM
                     SKAYVISCCALADERTIKMLQLTNDQEKYFHIPENTGGSLIVAPDAEIIAGPLGNEEA
                     ILYADVDLNLIIKRKLRQDFAGHYNRPDIFQLKINRTVPSIYNQEEQPDDHKNFQNAL
                     KVNQQFTDASNDTEPGFH"
     gene            11109..12182
                     /locus_tag="PLL88_07615"
     CDS             11109..12182
                     /locus_tag="PLL88_07615"
                     /inference="COORDINATES: protein motif:HMM:NF019003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HOJ01468.1"
                     /translation="MEILPNTGPQAEKFKNRLNQWLESVPQWHDNFVWTPNEKPAWCA
                     PGKPLAQSTVALIGTGGFHLKNQPPFDVDNPFGDASFRLIPACSDPADIAISHTHYDH
                     SEADHDINCMFPITRLWELKEQGVIGDVAENFYGLNGFIPNPARLISETAPQILSQLK
                     KDSAEVVLLTPDSANGHRAVGLLQDAFEKAGIVTISITLKPEVTLFMNIPRAAYIRYP
                     YGYSAGPAFQPDLQREIIRETLLLIDQISKPGTVVKLPYRWSGVKGDPTIQSAEPRTQ
                     LLINGIDQMIKLLEEIVVDMQNAQHTENSTMQPDPHKLAFYQSQMLRARKLASLFENE
                     AIDQVHSIRNLSGPIKYLREEQK"
BASE COUNT         3371 a         3242 c         2702 g         3152 t
ORIGIN      
        1 acagcatgga aatacgctca ccctcttcaa agccaacgga ctggaatatt ccagcagcag
       61 caatttcatt gagggaaaac tggcgggcga ggttttggtc acctctgaga tcaaaggcac
      121 caacctgcgc ctgcagaaca acacgctcac ccaaaccagc gtgtgctttg taggttttca
      181 aaatggagag cgcagcctgg gagtgatgct gccgataaag cgattgctga acgagcgcag
      241 ggtaagcgag ccgtttgagg gcagtttcct catccaggcg aacggtgcag aattcggaat
      301 ggaatggctg acctatcgct acagcaccag ttccaagaaa agctgcattt tataccggat
      361 catccagaat ccttatccct gcaaccagga ctgcgcagcc atcgaaacgg aacctggctg
      421 cgaatgcttc tgcgcaaatt tcctttatta cgatgagaac tgcatcccaa ccgagattaa
      481 tccaaactgc aagtgcgtgt gccggggtga aggagccagg ttcacgccga atggggatga
      541 ttcgccttga tccaactctc aagtttgttc ccaggcatcg gtttaccgat ccagaaaccc
      601 tgtccataca tgcagcccgc tttcagcaac caatcaaaat ctgcctgaga ttcaattcct
      661 tctgcaatcg tctgcatttc aatggaagca gccaatgaga gcgctgtatg cacgatctgt
      721 tgttttttcg gctctttatg aacacccaga acgaagcttt tatcgatctt catgtagtct
      781 actggcagca attgaatata ttcgagagaa gaataaccag taccaaaatc atccagcgca
      841 atgcgcgatc cgatcgctct caagcgtttc agcgcactga gcgatctttc gacatccttt
      901 ataatggcag tttcggtgat ctcaaaaatt atcctggagg catatagatc aaaggtatcc
      961 aagagactct cgaacctttc gatccaggaa tggtcaagca acacgcgcgt ggaaaggtta
     1021 atccccagat atccatcgaa atttttccaa ctagccaatt gagcgacaat ctcgcatatc
     1081 tcccaatcat gcagagagta cagaagtgaa gtatgttcca atgcgggtaa aaagctcatg
     1141 ggcggaatca gaccgagttt tccatgattc caacgcaaaa gcgcttcaac ccccattacc
     1201 tctccattct gcaagcgcac gatcggctga aaattgagtt tgaactcatg ccggtcaatt
     1261 ccctgcagta catcgcccag cagcgcctgg ctgttgcgcg cggtttggaa ttcttccctt
     1321 tcatagatcc aatatttcaa ttccttctcg cttgccacct gagctgcaac cgttgcattt
     1381 tgcagtgaga tatgcggaga catattcttg agattatcct gcgcgacgcc aatactgatg
     1441 ttaacgaata caggaacatc gttaatttca aagggttccc gcaatgcggc atagatagat
     1501 tcggcgataa aatcacaggt atttctttca tcgtcaattt ctacagcgag acacaataaa
     1561 tatggcagga tctgatagag gacggatgag tctcccagaa tgtttttcaa gcgatgaaat
     1621 accatcatgt tcaatttatt gagctcttcc atatccaaga tgcgggtgat gcgggcatga
     1681 ttgtcaacct ggatcatgaa aatagcgtat accgtgtcga gggatctatt ttccttcttc
     1741 tgttccaggt ccaaatatag atgggtgcgg ttgggtaacc ccgtatagcg gttatgcgaa
     1801 gcctgccaat gcagctcttt cacctgtctc ttgacagtcg caaaaactgc gccctgcatt
     1861 cccccaacca gcgtgaaaat gatcagccgg taaagccagt tatacagggt ttgcatttct
     1921 cctgtttcca ccaccatcgg catcgatggt ccaagaacca aaccaccaag aatccccgca
     1981 attacccctc caggcacatc aaagaaatat gcagccagaa agatggggat atacataaaa
     2041 tgtgaataca cgtatttgat cccgcccgtg taataaacca gtaatcccga aagcagaatc
     2101 aaaaccacaa caaggagcgc gtgccacatc cccccctgcc atatggagat tttcaaaaaa
     2161 tcctcattct ttatttggcg tttcgttttc tctgaccgca tcatcactcc cttggaccgg
     2221 acaacgataa acacgaagga ttaaaccatt atatatcaga tggggatgat gaataagctg
     2281 aatcaagctc gaagattctt tctatgaatc cattgatttc gagacgagca agccatgttg
     2341 acaaatcgaa atggtttcaa tgacgctgca ctcgagatcg atttggcgaa tggcaggggt
     2401 ggatgcattc cagcccgtta agcgatcaat gatttcaaca cagccatggc acatacgctt
     2461 tcaagtatct ccaccctatc gatgttttgc tccaacgttt gacctgctga caatacgcca
     2521 acagaaggaa ccagccagat ttcgttcgag tcttccaccc attccccatc gatgtagctg
     2581 gcagggatca atttcacccg ctcgatcaaa gcatctgcat cactcagaat tcctttgggg
     2641 ggcaattgat tcctgttcgt cagcaccatt aaaaaatgcg gttggcacaa caacacggtt
     2701 gtggcggcac tatagcgata aatctgttga tgccaggctg tatgtttggg tagcatcaat
     2761 tcttgatcgt gtggttgctg ctctattccg gtaaaaagga gcgcttcaac atccaacaag
     2821 ccgaaaggaa tgtctggcgc gttgatcata agcttgcccc cgcccacccg catgctcatt
     2881 tctgccagcg aacccgcgca aaagcgcttg gcatctacga ctctggcagc agccaggtac
     2941 cgttcaaaga tcacacgcga tgcgaccgca ctgcgtgtga tcttctgggt tttgcgaggt
     3001 gaatttacca gatcaacaac cttccacatc gctcgttgca atcgtttcag catatgcacc
     3061 aactataaaa tcgttctact caacataacc cagcgtttta tcctgaagcc agcgcaaatg
     3121 atagatggtc tgtgactcgt tcagttccaa ctcagcataa tgcagcggtt gtcctttttt
     3181 aacatctctg atgatggttg caccaacggt caaaccaagg ggtaccaaac cctccttgcg
     3241 cgccacttcg gctttttcaa cattcccata taccgtataa ccgcccaggt aatccacttt
     3301 ttcgccggct ttcaggtctc tctttgccat ggtgatggtc tcagcggtgg gtgcactatg
     3361 ggttgccagc gtttcttcgt gatagaagaa agcgcgcaag atggaaatgg gcgtttcaag
     3421 gtttgccata tgataggggc ggtacagtgc ccaataatta ccgtgatggc tgacatgcag
     3481 gtaattcaag tcagcgatga tcttgggctg gtcggtagtg atgatgacga acacacccgg
     3541 ggcaatctta cccatagcat aatcgacagc gtgcctcgtt tccagaatgc caccatcctt
     3601 tttcggacaa aacaccttcg gaagatcggc aacttccacg gtggggccat accctccacg
     3661 cacttcgggc acataaccgg tcccattcgc catcgcggtc atttccgcca tcgttttggt
     3721 gccatcctca aatgccgcca gcattttggg gttcatattt tgcgctttgg ctgattctgc
     3781 caccgtatca ggggttgcat caggatgcag cgggttgttc tttcctttcc caatgcaaac
     3841 aatttcaaaa cccaacgtat cggcaaagtc atacagctct ttgaccgagc ccggttcatc
     3901 accggctacc agggtataaa ccacgccggc tgcttccgcc atcttcgcca actggtagcc
     3961 aaccaccacg tctgtttcca cattcatatt gaccaccgac ttgcgcgcca ggatagcatc
     4021 gtagcagaac ttcgccccca cttccggtat gccggtcgat tcgaccacaa catccaggtt
     4081 agggatctga acgatcatat ctgaagaatg gcaagcaaca cgtttaccac tttcgatagc
     4141 ctgcgctgct ttttcggggt catcatcgtt tttcacgagt agcgttttat cgacactggc
     4201 ttcttgatac gcttcataag cacgatcttt ccgcacgtcc gcaaccgcga cgaccttgat
     4261 gccgtacatt ttttcgatct ggctgatcag cccggtaccc atttgaccgg cgcccagcag
     4321 acctacccgt acgggtcgct gattctcctt ttcgaattta tacaacaatt ttttaaagga
     4381 cattccaaat cctccaaaat tcaatttcct tcaatcttga tttctgtgcc aattgcaata
     4441 gccgggatct gtttttcttc caaatttaca tctccaggca gacgggctgt atcctctcca
     4501 ctgaacttga ggattaaatg tcccaaattc gccagatttg tattggcaac atctccaact
     4561 gacagcaccg tgaatccttc ggcgccgata tacacggtat ctccggattc gacaggtttt
     4621 atcagctctt tcgcatcgtg gatgattgaa aactccacca attcttcagg cgctttttct
     4681 ccaaaaaaaa ccagaatgcc agcctgtcta aattcatcga ccaatggacc gatcgagaca
     4741 acttttccct gaaatttgat catggtccac tcctttttta aattcgaaaa cagacttcca
     4801 tctttttttc atccggaagc tgctttcgcc agatgttaat gaacttttag caggttagtc
     4861 taacacttca atatcttcca cccgcacacc agaaacaaaa acatcttctg tgatgaaacg
     4921 gaaaagtgga ccagacggtg aagtagggta aatgtcgact gttggaactt tcttcatcgg
     4981 gtagacaccc accctggctg taccaccaca gtcgatcaca caaactgcaa tttgttcaaa
     5041 tggtgcgcga gatttaaagc catcaaaagg tgttccgccc gtcaattcgg caattttcgc
     5101 agctaacgga tgaataccac cacctgtaac agagtagatc agattctttt catcggtggg
     5161 ttcaatagtt aatggaccac cccacccctt aggaccagcc ttgatcttga tcttcttata
     5221 tttcttatca gccatgtttc ggaacctctt ttttatctta catttcaatt ataataagca
     5281 tgtgtgtggt gaagggtggc agatcgcaag acctgccccc ttcacgcctt cttcccaatt
     5341 ctttaagccg aatacaatcc aaagcttgct aaccaggcca aaacaacagc aacaggtccg
     5401 gttacgaatc gggagaacaa tactgccgga acgccgattt cgacggtttc tggttcggct
     5461 tcacccagtg acaaacctac cgggatgaaa tcacaaccaa cctgcgggtt gatcgcgaac
     5521 agagccggaa gtgccatctg tgggggaatg gaaccttcgc cgatcagagt tcctaagagt
     5581 gtgccaataa tctgagcgat gaccgcgcca ggtcccagca gcggggaaag aacggggaac
     5641 ccaatgataa aggtcatgat caacaaaccg atcaagctgg aagacaacgg tgccagggtg
     5701 ttcgccagga aattaccaac accagtcgcc aggataacac cgatgatcat ggaaatgaac
     5761 gccatgaacg gtaatacgtt cttgatgacc tgatcgatcg attcacgacc cgcctggtag
     5821 agtatgccaa ctactttacc aactgctcgt ccgatttttt caagaaactt agccatgatt
     5881 ctctcctatt tcttttccgc cgctttgcga cccagcataa tggcagtgat cacttcggta
     5941 acaatacctc tgatcaaaat cacaaccaaa ccaaccagca gatagcggat agccagatcg
     6001 ccgataggta atccaagagc ggtgataccg gctgcaatac cgccccaaac gaaatactca
     6061 ccagggttaa catgcgggaa aagtcccagc ggaggatgca cgaaactaac agcagcatca
     6121 tagaaagctg gtttgtagcg ttcgggtaag aaacgcccca tggtatatgc catcgggttg
     6181 gtaaagaaga acactgctac aaaaggcaga atcatatagc gcaaaggata atagagaata
     6241 ccagggcgcg cagccacttt accaagattt tccacctttt cgggcccaat caatgcaatc
     6301 agtgcattga ctgcggtcat caggactacc aacagaggaa tgatcccagt gaccaaaccc
     6361 acgaactgtt cgccacctgc ctggaacact ccaatgaagc cctcagcaaa ttttgctaat
     6421 ccttccattg tgaataacct cctaagattt ttgtttttaa attaccgact tccaatatcc
     6481 cttattccat tgccttataa tcacctcctt ctttcatact accttcgagg aattctcatc
     6541 aagttgttca tcattttgtg cctgcgctgc cgcttcagcg gctttttttc tggcgtattc
     6601 cacttggtca acagcgttga taaaggcact acggatcttt ttcgatatgg gcagttccac
     6661 actttcatcc ttgatatcct ggagtgtgag cccttcaagt tcttttacgg gtttcaaact
     6721 ggcgaacacg gtccaaccgg agaattgttc ggcacgaacg atcttctcct ggtcatcgaa
     6781 caccagcacg ccataagcgc gtcgcttata catggagccc gacatgccga ccgccgtcgg
     6841 tcccattttc ttcaactcag ccacgcgctt gttgtatcga cgcaattgaa agaaggtcat
     6901 cccgaattga atgacccaag cgacaataaa aacgaggaaa attgctgaac ccaattgcat
     6961 ctcagctccg aatttaatat tattcctgtt gctctaccag tcttctacca atctccgaat
     7021 cagtgatcag aacactgagt aaattaccaa tgagcgatgc ttttaccgct tcaaattttt
     7081 caggaccgcc cgcgatccca accaattttt tgatactttt atattgttcg aaatccaacc
     7141 cgatgatgcg gtcgttgatt tcgttctgaa tttccttgcc atttgcatcc ataaaccgta
     7201 accccacatc ccccaccgcc ccctgctttt ccaattcaac cagttcattg ggagaaagga
     7261 tgccgtactt gatcaccggt gaactggaag ttggtcgtcc cagacccacg atgccaatat
     7321 cagcccttct gcccaactcg agggtttctt cgatttgctg ctctttccgc aacgcatcac
     7381 aaatctcttt ggtcgggaca attcccgggg ctgataaaag gcgcgctttc gccccaaatg
     7441 tctgcgccat acgcatggtc aggtcagcgg catacatatc tgcctgtgac gatcccaagc
     7501 cacccaacag ttggacgata cgcatagctg gccagttgtt gctgtgcagg gcatccacca
     7561 tcgccagcat ggttccaccc caggtgatcc caataatctc atcccccgaa atgacccggt
     7621 ctaaaaaatc agcggtcgcc tgaccgatcg cttgcagcgt ttgctgatga tctgttcgcg
     7681 caggcaccag gatgacctca tccacatggt actttttttc gatgcggttt tccaattcga
     7741 catccgcatt gatcttgggt tcctgaatgg ttatctgtac cagtttttct cgacgcgctt
     7801 tttccaataa tcgccccact ttaatgcgcg acagtccgaa cttagaccct atttcctgtt
     7861 gagtcaatcc atcatcataa taggcgcgag caattttata catgagtttt tgtgtgattg
     7921 gatcggtcgc cataagtgat caaatgctca taactgttct tttgttcaat tgttattata
     7981 gcatgtttca aattgaaaat caagtatttc ttcactgcaa aggtcgccca ctcctaaaaa
     8041 tcagcatctt ctccggattt tttctcacga agcacaaaac cagcacattc atacacttat
     8101 gcaaaaatgt gataaaaatg aaatcatcat gaacgacatg aacgccgtga tctacaaagg
     8161 accgggggat tacacactcg aaaaagtgcc cgttcccttc atctccgata aagaagtgtt
     8221 gctaaagatc gatgcggtag gtctgtgcgg atcagacctg cgcacgctca agtttgggca
     8281 ccgcaaggtt actccaccct atattctagg gcatgaaatc agcggcacca tacgggcagc
     8341 aggagcgctg gtcgacccac aatgggcagt tggcatgcgg gtagcgctgg ctccggtggt
     8401 ctattgcggc aactgccatt tttgccagcg ggggcaattc gaactgtgcc agcactaccg
     8461 cgagatcggg cagagctggt caggagggtt tgccgattac ctcaacattc cgcaagatgc
     8521 gctgcagcat ggcgtcatcc accccatccc ggcaagtatg gatgccgtac acgccaccct
     8581 ggctgaacca ctggctgcct gcctgcacgc catagatctg gtggacctgc gcaatgtgta
     8641 tacagccgct atcttcggct ccggcgcaat cggctgcttg ctgctgcaat tgctgcgcaa
     8701 tcaagggctg cgttctatca tcatcatcga tcccaacgaa agccgtttgc gcatcgctga
     8761 gcattttcgt cccgatcaca gcatcaacgc caacagtgag aaagctgttg agaagatctg
     8821 ctcgcttacc ggggggctag gcgttgatct catcttcaca gccacagcag caccggttgt
     8881 gcaacagcag gcactggata gcatcagcaa gggtggtcaa attgtggtgt tcgccggttt
     8941 gccgaaaggg caatctgtga tcccgttcga tctcaacaag gtgcattacg attgcatccg
     9001 cctgatcggc tccagcatct acgcccctca ccaccatgag gaagccttac gtttgatcgc
     9061 cgaacaaaaa atcgatgtaa agcgtatcat taccaccttc cccttggcta aatttcatga
     9121 aggcgtacag gcagccatat cgggcgagat cgtcaaggcg gtgttcatac cctgatcatg
     9181 ttctctaagc gattggaatt gttgagcgtc aaccaggacc aagaaccggc gggctgagaa
     9241 ttttccgaag cagaggagca ttgaaaatgc gtgcgcactg gtattgcgat gagcaacccg
     9301 ctaccgatga agttttttcc aagggaaaac accatcttta caacgcccca tcttcaataa
     9361 aaatggtcgt cccttgaagg ggatcaacgg catggaaatt ttccgtcgtc aggaaacgcc
     9421 ataaaaacta cagggctccc tgattttttc tttcaatcaa acggtcgctc aggcagcgct
     9481 caataataac atttgagagc cagcgcgacg gtgaaaatga cataagcgat gagggcaaag
     9541 gtgaggaaca gcctgaacca ggcagcaaaa gcgctcccgt atcttttctt gcgcagattc
     9601 caattattgt cagggtcctt atccctgcca acccctgaaa gcggacccca catgaaagcg
     9661 gagagaacgc cgatgatgag actggggaag atggaacgaa cgaagctact gatgacgcag
     9721 gtagacatgc tttttctcct tcggtttgtt tatcaataac aaatacgcga tacccataaa
     9781 gcttgtgcac caattatata tcgttttggt ctttcatttt attgtcatgt gcgtatccat
     9841 cacttcaccg tcctataaag acaccgcaaa cgcccagaac cttaccgcaa aataaagttt
     9901 caactaccac caagttgtcc atggatattt catgttacaa tcttcaacag tttagtgtta
     9961 atgtttaatg ttaatgagga atagaaatgg gagatcaatt tccatctttc aaaatcgcag
    10021 cagttcaggc tgctcctgtc tttctggatc gcgaggcaac aactgaaaaa gcctgtcaat
    10081 tgatcaggga agcggctgca aaaggagcca ggattgtcgg cttcccagaa ggattcatac
    10141 cagcgcatcc aatttggttt cattttcatc catcaactga caaaatcagc acacaattaa
    10201 gtgttgcgct cttcaagaac tctgtggaaa ttccaggtcc gcaaattgcg caattatgcc
    10261 aggtggctaa agagacgaag acttatgttg taatgggcgt ttgtgaaaga aaccctggaa
    10321 ccatggggac catgtataat acccaggttt ttattggcgc cgacgggagt gttctcggga
    10381 aacatcaaaa aattatgccc accacaggtg aaaagttggt tcatactggc gggtatggcg
    10441 acaccttgag aacttttccc acagagtttg gtccaatcag cggcttgatt tgtggcgaaa
    10501 acacaaaccc gctggctatt ttcgccctta tagcggaatt cactagaatt cacatcgctt
    10561 cctggcccag caattttacg ctcatcagcc atccgatgcg agaaagaatg agttttaccg
    10621 ctaaagcctt tgcggaaatg tcaaaagctt atgtgattag ctgctgcgct ttagcagatg
    10681 agcgcaccat caagatgttg caattgacga atgatcagga aaaatatttt cacataccag
    10741 aaaatactgg agggtcatta attgtcgctc ccgatgccga gatcattgcg ggtcctttag
    10801 gaaatgaaga agcgatctta tacgcagatg ttgatcttaa cctgatcatc aaacgaaaat
    10861 tacgacagga ttttgcaggt cactacaacc gacccgacat atttcaactc aaaataaacc
    10921 gaacggttcc ctccatctac aatcaagaag aacaacctga cgatcacaaa aattttcaaa
    10981 atgcgttaaa agttaaccaa cagttcactg atgcttctaa cgatacggag cccggttttc
    11041 actagaagcc acctctaaga aaaatgaaca ttgctaaaat catgaaaaac tctttacagg
    11101 aataaaccat ggaaatcctc ccaaacactg gacctcaggc agaaaaattt aaaaatcggt
    11161 tgaaccagtg gcttgaaagc gttccacaat ggcatgacaa ttttgtttgg acaccgaatg
    11221 aaaaacctgc ctggtgcgca ccaggcaaac cgctcgccca atcaacagta gctctcatcg
    11281 gcaccggtgg cttccatttg aaaaaccagc cgccttttga tgtggacaat ccctttggag
    11341 atgccagttt tcgccttata ccggcatgct ctgatccggc tgacatcgcg atatcccata
    11401 ctcattatga ccattcagaa gccgatcatg acataaactg catgttcccg ataacccgct
    11461 tgtgggagct taaagaacag ggggttatcg gcgacgtcgc tgagaatttc tatggattga
    11521 acggttttat acccaaccct gctcgattga tcagtgaaac tgccccgcaa atcttaagtc
    11581 aattgaagaa agatagtgcc gaagtcgtct tattgacccc cgacagcgct aatggtcacc
    11641 gggctgttgg tttgctacaa gatgcttttg agaaagcagg gattgtgacg atctccatca
    11701 cgctaaagcc tgaagtaacc ttgttcatga atattcccag agccgcttat attcgctatc
    11761 cctatggcta ttcagccggc ccggcttttc agcctgatct gcaaagagag atcatacgtg
    11821 aaacgctctt gctgatcgac cagatcagca aaccaggaac cgtggttaaa cttccttacc
    11881 gatggtcggg cgtgaagggg gatcctacca ttcaaagtgc cgaaccacgt acccaactgc
    11941 tgatcaacgg aatcgaccaa atgatcaagc tgctagaaga aattgttgtt gacatgcaga
    12001 acgctcaaca cactgaaaat tctaccatgc agcctgatcc gcataaactc gccttctacc
    12061 aatcccaaat gttgcgcgcc agaaaacttg ccagcttgtt tgagaacgaa gcaatcgacc
    12121 aggtgcacag catccgcaac ttatccggac cgatcaaata tctgcgcgaa gaacaaaagt
    12181 aagaattgtc aggtcgcacc tggttgtttt cgtatcttct taataaattt tctttttcaa
    12241 tgaatcaaat tttcgtacgg aactgtggag tttgcataat acctaaagga agtataggca
    12301 taccaaccag ccttccgatg tttgcatgat aatgaagcat cacggcaaag taccgcattg
    12361 agcttgaaac gcttagctcg tctggagaag gttgtcattg tttagtatca ggaaagaaaa
    12421 ggatcaacgc acttttaaaa cacaagtagc gcgggtggga cttgaac
//
LOCUS       DAOSDS010000058        12459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_1956_length_12459_cov_5.210174, whole genome shotgun sequence.
ACCESSION   DAOSDS010000058 DAOSDS010000000
VERSION     DAOSDS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12459
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1956_length_12459_cov_5.210174"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLL88_07620"
     CDS             <1..347
                     /locus_tag="PLL88_07620"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOJ01469.1"
                     /translation="RPAEINVQELCEADSKQILTIIDPDNQEVKSSRAFPVWGPRSPL
                     SVLLSHDEGDTWHVVADIEDAVGEYSYPAVIQSGEKQIAVAYTANRTEIRFRQFTIPD
                     RFAGKTFLTNAG"
     gene            351..815
                     /locus_tag="PLL88_07625"
     CDS             351..815
                     /locus_tag="PLL88_07625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOJ01470.1"
                     /translation="MIKETLIRPLEALELPAHVKRALASLAKIDFSSMKEGEYMLEDG
                     SKLIFSTYETRPVDKQVRVEGHKKFIDIQMILEGEETIGVLSADQIENKSEYDETNDI
                     WTAEIDAEKVEFIDLKKGEFLVLFPMDAHAPQLAARNVPAPVKKGVIKVLSR"
     gene            1121..1903
                     /locus_tag="PLL88_07630"
     CDS             1121..1903
                     /locus_tag="PLL88_07630"
                     /inference="COORDINATES: protein motif:HMM:NF019359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOJ01471.1"
                     /translation="MEPVQRTYSIKELASMAGISIRTLHYYDAIGLLVPNRHPDNQYR
                     CYEHSHLLKLQQILFYRELDFPLEQIKNILADPSFELKTALAEQREMLIEREKKIARL
                     IDTIDKTIMQLEGEEVMEDQALYSGFSEERQKEYEKEIRQKYGDEPLNTSMQRWNRLS
                     KAQQKEMIAKGQQFHQKIADVMPLGHESHEALELMRQYWENMQFFYDVSLERFEQLGH
                     MYNQDPAFRSTYETVKPGLAAFMEKAVINFVRVEKQKEGSHE"
     gene            complement(1949..4144)
                     /locus_tag="PLL88_07635"
     CDS             complement(1949..4144)
                     /locus_tag="PLL88_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01472.1"
                     /translation="MDKDILLFENDLRAAIAPPNAPNDFVNALGVRLLNSYPSSKEKV
                     RRNIRLQPRWIVLICILVLFIVSVLLIGPDKVYAEVLKLLGYVPGIGIVDQAQSIRVL
                     PEPVRITRDGVTISVNQALLTPQQTHIEYGVSGVPASAYPEQEAVSGCVEQEYILLPD
                     GTKIATDDPIPMDVNQITLVIPCIFNTLPDTVPTDWQIPLTFIPLPADTTVFPVQELT
                     PDSTTSTSIPTLAPDTRSSSSFEFNKVITTADGYILIGWFRPHLAENEQLQVNGVQYI
                     DANKNVVKVDYPQDIDLSSSGMPFGVGDSQWIIQFKASDVAFPLIIRYTGKIYTRAEA
                     EPLEFEFDAGPNPQSGQEWNVNQDFEIAGISFRLVSIRANKNGYSFQFESDPELDGLN
                     VQIDGYQPMGGGGGPQVRSLDFESIPTGKLKLIFSNFYQFTENRTWEGTWQPDLLPAS
                     QPTSSSAPPICVDANTYATLPSLPAGLEGWVLLTELDPERRLVLQSMDGEEAKLTIPQ
                     TGRGSLSPDGTKIAHTGKEGTVIIDIASGHSFTLPHSGGIDWSPDGKLIATVNPAAGY
                     GIFVSAIDGSSTHRLTNLGYEAIAGWSADGSTLYYAVPDAGGEGFMLRAVDIKTGSAR
                     DLFILEDSSGKAPYAAVSPDGKWVVYRARDNSSLYLKRMDGSPARLLLDRPATAISSM
                     VWERDSHLLGISLITPETSDGEIILMQFENCETYKLPGLHGELDGIFIP"
     gene            complement(4137..4712)
                     /locus_tag="PLL88_07640"
     CDS             complement(4137..4712)
                     /locus_tag="PLL88_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ01473.1"
                     /translation="MTKSQTPDSISGKPQTDDELVLIKKAKQDAGAFEELYKQHVQAI
                     YKYCYNRTGEQVEAEDLTEQTFMAALEGIQQYRDDGHFAAWLFSIARRKVADYFRSKD
                     RLSSQEIPEDIPMHSDFLGDVIRSERQKAISEIFNALPENKKELIRLRYVADLNFATI
                     AQLLHRSEGSVKKATYRTIDQIRKKMEERYG"
     gene            complement(5129..5779)
                     /locus_tag="PLL88_07645"
     CDS             complement(5129..5779)
                     /locus_tag="PLL88_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ01474.1"
                     /translation="MDYLSYKKEVFDTTLKLVEIDLIRLSSGNISVRLPDNNIAITPS
                     GVPYDQMAPEDIVIMDLTGKKLEGEFKPSSEKALHNEIYKAKPDVHAVVHTHSVYAIA
                     FSSVGMELPVMCIELLSAGAPIPVAAYRCPGTEEVGIDAAQYFVDRPRLKGLLLKNHG
                     LVAIGKNLNDAYQNAYKIETGAEIYHLALQTGVKPTPLNKEQIEEALERYQAPKEK"
     gene            complement(5821..6561)
                     /locus_tag="PLL88_07650"
     CDS             complement(5821..6561)
                     /locus_tag="PLL88_07650"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ01475.1"
                     /translation="MFPEIDNIPLKGVPRYVQIREDIRRKIEKREIKVGTQLPSEADL
                     AEMFGVSRMTVRRAIEDLVMDGLLIRKQGTGTIVSSRKVVRDYTKLTSFHEDAQSRGM
                     HPISRVLKFEVILPPADYVEKLMIKETDKVFHIIRLRMVDDNQIIALHETFIPQPLCP
                     WIENANLENESLYELYKKHGLAIEWGKQIVEARSVTEEQAQCLQISMGSPVLFSERIS
                     YTVNNVPVEVVAGVCPGDRFSINLILRR"
     gene            complement(6565..7593)
                     /locus_tag="PLL88_07655"
     CDS             complement(6565..7593)
                     /locus_tag="PLL88_07655"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ01476.1"
                     /translation="MKAFISSRITDQWLVELKKQFSDLELYDWFARGMLSTQEFEKRI
                     EGCHLVVTESDTITAEMIRKAENLFAIVDFRGTVINVDIDAANEKGVVILNTPGRNAD
                     AVADLTIALMIMAARNVLRGIDAFRQGLWTEKGRYWVYQNHQGYDLPGKTVGLIGLGA
                     IGRLVTRRLSGFNVKIIGYDPFVSDEEALKMGVKKASLNEVFQKSDFLSLHLPLNSQT
                     KNMFDEKELRMMKPNAYLINTSRAGVFQEDALVRCLKEGWIAGAALDVFHSEPLDSTY
                     PLLNLPNVICTPHLGGATYDVIEHQSQTGVGGLLEFLHGNIPDNIINPEAIEKSIVRY
                     KNYARAGG"
     gene            complement(7604..8491)
                     /locus_tag="PLL88_07660"
     CDS             complement(7604..8491)
                     /locus_tag="PLL88_07660"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ01477.1"
                     /translation="MNTIIDTHMHIGKESLLSADIVSFMKSKNIWKNIKEKISPEGII
                     RTLDEGKISQGVIFPLTFMPPDSQWQKLNDLTAMYIQQYPGRLIGYGIINPQDVSGSL
                     KELDRCFDELGFRGVKLHPSMQEFYPNQERLFPLYEYCQQKQVPILFHTGASLASHSD
                     KFSHPILLDDIAVRFPELSMIIAHVGRPYYQDAALLLRKHTNVYADICANSGRTGGTY
                     LLEQVLTWIKVYADGIKKLLFASDFPVFDPTNTLNDLENIFKTSRFPNSKDKLITQEE
                     WNLLTHWNAEKLIQEKANG"
     gene            complement(8488..10014)
                     /locus_tag="PLL88_07665"
     CDS             complement(8488..10014)
                     /locus_tag="PLL88_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ01478.1"
                     /translation="MVKRRQFLMALDFGTGAGRCYLISVDGQQSFEQYSEWEYSYPPE
                     AQPGGSEFNAEEFWDILSNLIRRTIKKAGIDPAQIIAVSSTSQREGVVFLDKNGKELY
                     AGPNLDMRAPRNSSEFEAQFAENIHASSGHWPMPMFLPYRLLWFKENKPEIYEKIDSI
                     VLLNNWILFRLSGERITDPSNGVETLLVDLKKRDWNSPLIKKLGFPERYFPPVYESGT
                     FIGKVTTKAAAETGLSAGTAVVSGGADSQCGLLGNGAVAEGEIGVTLGTYGPLQMVLN
                     NPVIAKPELTWSGCHVVPHAWVIESTSMEAGQTFRWIRDIFYANEIGDVYTLMEKEAS
                     TAPPGSNGLKAYIGPRLPNYRNLDFNCSGGFVTQLPATPGRVARKDFSRAALEAVAYG
                     VKLNVDRLQRVAGLALLDLRACGGLTKSRTLLQIMANLLQVPVFVPKQKEGSALGAAI
                     CAGVGANIFKSMIEGAQVCVKEDLLLQPEYESGSVYQELFADWKQHYKTMYGERILEA
                     "
     gene            complement(10029..11006)
                     /locus_tag="PLL88_07670"
     CDS             complement(10029..11006)
                     /locus_tag="PLL88_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01479.1"
                     /translation="MNGPLATPNKALTRGRKNNFGTLAGFAVFALVFMYFGINAPGFL
                     TQVNIVRNLILPATISAVISIGMTIVMSGGGIDLSVASIAGLAGMVAAVGSAKLDMSI
                     ALMILVALITGALIGALNGFLVAYCGISPFVVTLSVIYLARGLQYLVALSTVSGTYLM
                     LPRDAVALGGKPAFFISAAVMVAVFLYVFLDHTIYGRFIRTVGSNMHAARYSGIPIRF
                     YTWITYVICGLCCAVTGIMLTFNEGMAQVGSGDSYQIDAFLLPILGNAIFSRFSVQGT
                     IFSSLFLYMIINGLFILGTPPEYLRIIKGALLITIILVSGLQKIISKNK"
     gene            complement(11023..11973)
                     /locus_tag="PLL88_07675"
     CDS             complement(11023..11973)
                     /locus_tag="PLL88_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01480.1"
                     /translation="MKTAKNAVRKNAVFGILIILFLTFAFLKPSFLKVDNLLNLLVQS
                     TILGIVGFGLAIIMMAGEIDLSYAGAIPLEGAIFANLLQKDMGVWPSLGVSFLAGITI
                     SLVISQLVTRLKLVSYVTTTSMYFLLQGIWYAYTGGENVYFKEKFNRDLIFGSLAGIP
                     KIVIFFLLLFVILYFLTEHTSFGIRLRAVGEDPEAAKTMGVNSITYKTAGFILGGFIF
                     AIGAFFSTAKMSGALATSGANMLMPVMTVAFVGQTFLGMGRPNIPGVFLAALMMAMID
                     NAFVLMNSPFWAVPIANGIILILAIFLANVGKRDIIQIKL"
     gene            complement(11960..>12459)
                     /locus_tag="PLL88_07680"
     CDS             complement(11960..>12459)
                     /locus_tag="PLL88_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ01481.1"
                     /translation="RKYHGLLLDNSIQSNITLAGLRNFTTSRFFTSVQKERDIVIKLV
                     HRLHIATPSIEKHTRELSGGNQQKVVLAKWLCTTSKIFIFDEPTVGVDVQGKTEIYNL
                     MHELADNGAGVLFVTSDVEEGLQVCDRIAVMYKGSILTELDPLKTSIEEVSMFAMGGH
                     PDENR"
BASE COUNT         3313 a         2885 c         2825 g         3436 t
ORIGIN      
        1 accgacctgc tgaaatcaat gtgcaggaat tatgtgaggc ggattcgaaa caaattttga
       61 ccatcatcga tccggataac caggaagtaa agtcttcacg tgcctttccg gtttggggac
      121 ctcgcagtcc gttgagcgtc ttgttgtcac atgatgaagg ggatacgtgg catgtggttg
      181 ctgatattga agacgctgtg ggtgagtaca gctacccagc ggtaattcaa agcggtgaaa
      241 aacagatagc cgttgcctat accgcaaatc gcacagagat tcgtttcaga caatttacta
      301 tccccgatcg tttcgcagga aagacatttt taacgaatgc aggataaaag atgataaaag
      361 aaacgcttat tcgccctctt gaagcacttg agctaccggc tcatgtaaaa agagcactgg
      421 catcgcttgc gaagattgat ttttcttcca tgaaagaagg ggaatatatg ctggaagatg
      481 gaagcaaatt gattttctcc acctacgaaa cacgaccggt cgacaaacaa gtgcgggttg
      541 agggacataa aaaatttatt gatatccaga tgattcttga aggcgaggaa acgattggcg
      601 tgttgtctgc tgaccagatc gagaacaaat cggaatacga tgaaacgaat gatatttgga
      661 ctgcggaaat cgatgctgaa aaagtggaat tcatagactt gaaaaagggg gagtttcttg
      721 tcttgtttcc gatggatgca catgcccctc aattggctgc cagaaacgtc cctgcacctg
      781 tgaaaaaagg tgtgatcaaa gttctctctc gatagtttat gtggacagat cattgtatat
      841 ctgaaagtta accagatact gcaagggatc cctgtatatt ccggaggcgt tcaggtgagt
      901 tatgcacacc tacaggagct gcattattaa agcattttgc cgatcgttcc tccaagatgc
      961 ccgtcatgca agtcaatgcc gtttcgtatg gcatggggaa aaggaatttc gaagctgtta
     1021 atggtgtgcg cgctttctgg gaagagagcg cagtgaaaaa tgttcctgtt cttgtcgccc
     1081 tcttgacatt aaccttacgt catcgtttat gattgtttgc atggaacccg tacagcgaac
     1141 ctattcgatc aaggagctgg cgagcatggc aggcatcagc atacgcactt tgcattatta
     1201 tgatgcgatc gggttgctgg ttcccaatcg ccaccctgat aatcagtatc gttgctatga
     1261 gcattcccat ttgctgaaat tgcagcagat tctgttctac cgggagttgg atttcccgct
     1321 cgaacagatc aagaatattc ttgctgatcc ttctttcgaa ctgaagacag ccctggcgga
     1381 gcagcgagaa atgctgatcg agcgtgagaa aaagatcgct cgattaattg atacgattga
     1441 taaaacgatc atgcaattgg aaggagagga agtcatggaa gaccaagccc tttactcggg
     1501 tttttcggaa gaaagacaaa aagagtatga aaaggagatc cgccaaaaat atggcgatga
     1561 gccattgaat accagtatgc aacgatggaa taggctatcc aaagcgcaac agaaagaaat
     1621 gattgccaag gggcagcagt ttcatcagaa aattgccgat gtcatgccgc ttggtcatga
     1681 aagtcatgaa gcgttggagt taatgcgtca atattgggaa aacatgcagt tcttttacga
     1741 tgtcagcctt gagcgctttg agcagttggg tcatatgtac aatcaagacc cagctttccg
     1801 ctctacctat gagacggtga agcccggctt ggcagcattc atggaaaaag ctgtgatcaa
     1861 ctttgtcagg gtagaaaagc aaaaggaggg atcgcatgag tgaattgcag cgccttctat
     1921 ggcgctgcaa ttttttctct ctctgttctt acgggatgaa aatcccatcc agctcaccgt
     1981 gcaaacccgg taatttatac gtttcacagt tctcaaactg cattaagatg atctctccgt
     2041 ctgacgtttc aggcgtgatc aagctgatcc cgagcaaatg gctgtcccgt tcccaaacca
     2101 tgctgctgat tgccgttgca gggcgatcaa gcaagagccg ggcagggctg ccatccatcc
     2161 tcttcaaata gagactgctg ttatctctgg cgcgatagac gacccatttt ccatccggag
     2221 atacggcggc gtatggcgct ttgcccgaag aatcttccag gataaacaga tcacgggcgc
     2281 tgcctgtctt gatatcgact gcgcgcagca taaagccttc gccaccagca tcaggaactg
     2341 cgtaatagag cgtgctgcca tccgctgacc agccggcaat ggcttcataa cccagattgg
     2401 taagccggtg cgtgctgctg ccatcaatgg cgctgacgaa gatcccataa cctgccgcag
     2461 ggttcacggt ggcgatgagc ttgccatcgg gcgaccaatc aattccgcca ctgtggggga
     2521 gggtaaacga gtgaccactg gcaatatcta tgatcacagt gccctctttg cccgtgtggg
     2581 cgatctttgt gccatcgggg ctcaagcttc cccttccggt ttgcgggatg gtgagttttg
     2641 cctcttctcc atccatgctt tgtagaacca atcttctctc tggatccaat tcggtgagca
     2701 acacccaccc ctccaatcca gcgggcagag atggcagggt tgcataggta ttcgcgtcca
     2761 cacagatcgg gggagcgctg gaggaggtgg gctgggatgc aggcaataaa tcgggctgcc
     2821 aggttccttc ccaggtacgg ttttccgtga actggtagaa attagagaag atcagcttca
     2881 acttcccggt tgggatgctt tcaaaatcca aactccgcac ttgaggtccc ccgcctccgc
     2941 ccattggctg gtatccatct atttggacgt tcagcccgtc caattccggg tcactttcaa
     3001 attggaagga atatccgttc ttattggctc gaatagagac aagccggaaa gagatccctg
     3061 cgatttcaaa atcctgattt acattccatt cctgacctga ctgaggattg ggtcccgcat
     3121 cgaattcgaa ttcaagcggt tccgcttctg cacgcgtgta tatttttccg gtataccgaa
     3181 tgatcagcgg gaaagcgaca tcgctggctt tgaattggat gatccattgt gaatctccca
     3241 ctccaaacgg catgccgctg gaggaaagat caatatcctg gggataatcg acctttacga
     3301 catttttgtt ggcatcgata tactgcacgc cattgacctg gagttgttca ttttcagcca
     3361 ggtgtgggcg gaaccagccg atcaaaatgt aaccgtctgc tgtggtgatg accttgttga
     3421 attcaaagga tgaagatgac cgggtatcgg gtgccaatgt tggaatgctg gtggaggttg
     3481 tggaatctgg ggtcagttct tgtaccggga agacagttgt atcggcaggc agcggaatga
     3541 aggtgagcgg aatctgccaa tcggttggaa cagtatccgg cagcgtgttg aagatgcagg
     3601 gaatcacgag ggtaatttga ttcacgtcca ttggaattgg atcgtctgtg gcaatctttg
     3661 ttccatcggg taacaaaata tactcctgct caacacagcc acttaccgct tcttgctctg
     3721 gataagcaga tgccggtaca cccgatacgc catactcaat gtgcgtttgc tggggagtga
     3781 gcagcgcttg atttacggaa atggtgaccc catcgcgcgt gatccgaacg ggctctggca
     3841 gaactcgaat ggattgcgct tgatctacga tgccgattcc gggaacatat cccagcagct
     3901 tcaagacttc tgcatacact ttatctggtc cgatcagcaa aacactgaca atgaaaagga
     3961 ccaggatgca gatcaaaacg atccagcggg gctgcaaccg tatatttcga cgaacttttt
     4021 cctttgaaga agggtagctg ttcaataagc gaactcccag ggcgtttacg aaatcattgg
     4081 gtgcattcgg aggtgcgatc gcagcgcgca gatcattttc aaaaaggaga atatctttat
     4141 ccatatcttt cctccatctt ctttcttatc tggtcgatgg tccgataggt ggcttttttc
     4201 actgatcctt cgctgcggtg cagaagctgc gcgattgtcg cgaagttgag atctgccaca
     4261 tagcgcaagc ggatcagttc tttcttgttt tcaggcagcg cattgaatat ttctgaaatt
     4321 gctttctgtc gctctgaccg gatgacatcg cccaaaaaat cgctatgcat cggaatgtct
     4381 tcgggaattt cctgggagga taaccggtct ttggatcgga aataatcggc tacttttctt
     4441 ctggcaatgg agaacaacca ggctgcgaag tgcccatcgt ctcgatattg ctgaattccc
     4501 tcaagcgcag ccatgaaggt ttgttcggtg agatcctctg cttcaacttg ctccccggtg
     4561 cggttgtagc aatatttgta gattgcttgc acatgctgtt tgtacagctc ttcgaaagct
     4621 ccggcatctt gtttggcttt tttgatcaat acaagctcgt catccgtttg tggtttgccc
     4681 gaaatcgaat caggagtttg agatttcgtc atgttcctcc tttatttcat gcttctttta
     4741 attaaaatcg gggaatatga gaaaaaggga caaaaatcta tacaagagtg ttctttcata
     4801 aaatatagca aaaaatgaaa gaattgtcct attctctgct ataaacaaaa cgatgatgta
     4861 tggaaatatt tatggaatgc ccttttgttc caacgtttct gtgccgaaaa attctggcgg
     4921 gttacctgcc ggtgaaaggt cgcagaccgt acagaattcc cgccctacaa agagcacgag
     4981 attcctatac ggccatggcg cacttctgac gacagcgggg taggcgggat gaaaaaatta
     5041 tccgggcgat gaagccgcga tcaccaggga cctgtcccgc tggtttccaa ctcttttatg
     5101 aaattcgaac tgttgtaaaa aacgcccttt atttttcttt tggagcctga taacgctcaa
     5161 gagcctcttc aatttgctcc ttgttcagtg gtgtaggttt aacgcctgtt tgaagagcga
     5221 gatggtagat ctcggctccg gtttcgatct tgtacgcgtt ctggtaagcg tcattcaaat
     5281 tttttccaat ggcgaccaac ccatgatttt tcagaagtaa cccttttaac cttggacgat
     5341 cgacaaaata ctgtgccgca tctatgccaa cttcttctgt tccagggcaa cgatatgctg
     5401 caaccggaat tggcgcacct gctgaaagca gctcaataca catgacaggc aattccattc
     5461 ccacggatga aaatgctatt gcataaactg agtgggtatg aacaacagca tgcacatccg
     5521 gtttggcttt ataaatttca ttatgcaagg ctttttctga agaaggctta aattcaccct
     5581 cgagtttttt cccggtcaga tccatgatga cgatgtcttc tggagccatc tgatcgtatg
     5641 gtactcctga aggagtgatt gctatattat tatcaggaag acgtacgctg atgttccctg
     5701 atgatagtcg tatcaaatca atttcaacca gttttaacgt ggtgtcgaac acttctttct
     5761 tgtaggataa ataatccatg atattgtttc cctttcaaac tcaaaataag tataaaaatt
     5821 ttatcttcgc aagatcaaat tgatcgaaaa acgatctccc ggacaaacgc ctgccacaac
     5881 ttcgaccgga acattgttta cggtataaga aattctttca gaaaataaaa ccggtgagcc
     5941 catggatatt tgcaaacatt gtgcctgctc ttccgttaca gaacgcgctt ccacaatttg
     6001 tttgccccat tcgattgcta agccatgctt tttgtaaagc tcatagagtg actcgttttc
     6061 aagatttgcg ttctcaatcc aagggcagag gggttgcggg atgaacgttt catgtaaagc
     6121 aataatttgg ttgtcatcca ccattcgaag tcggatgata tgaaaaactt tgtcggtttc
     6181 tttgatcatt agtttttcca cataatcagc agggggcagg atcacttcga atttcagcac
     6241 gcgcgatatg ggatgcattc cccgagattg tgcatcttca tgaaaactgg tcaattttgt
     6301 ataatcgcgc acgactttgc gtgaggatac aatggtgccg gtgccttgtt tgcggatcag
     6361 cagaccatcc atgacgaggt cttcgatcgc acggcgcact gtcatacggc ttaccccaaa
     6421 catttcagcc agatctgctt cggagggtag ttgtgttccc actttgattt cacgtttttc
     6481 aatttttctt cggatgtctt ctcgaatttg aacgtaccgt ggcacccctt ttaatgggat
     6541 attgtcgatt tctggaaaca tagattaacc tcccgccctt gcataatttt tatagcgaac
     6601 gatagatttt tcaatcgctt cagggttaat tatattatca ggaatgttcc catgcagaaa
     6661 ctccaacaaa cctcccactc cagtttgtga ctggtgttca atgacatcgt atgtggcacc
     6721 acccaaatga ggcgtgcaga tcacattggg caagttgagg agtgggtaag tgctatcgag
     6781 aggttctgag tgaaaaacat ccagtgctgc ccctgcaatc cacccttcct tcaaacaacg
     6841 taccaaagcg tcttcctgga aaacgcccgc gcgcgacgtg ttaatcagat acgcattagg
     6901 tttcatcatg cgcaattctt tttcatcgaa catatttttt gtttgactgt tcaaggggag
     6961 gtgcaaagaa agaaaatcgg atttttgaaa gacctcgttg agacttgcct tttttactcc
     7021 catctttaat gcttcttcgt cgctaacaaa aggatcgtag ccaataattt ttacattaaa
     7081 accggataag cgtcttgtca ccagacgacc gatagctccc aacccgatca gtcctaccgt
     7141 ttttcctggg aggtcgtagc cttggtggtt ctggtaaacc cagtatcgtc ccttctcagt
     7201 ccataaacct tgccgaaagg catcgatacc gcgcagaacg ttgcgcgcag ccataatcat
     7261 caacgcgatg gtaagatcgg ctacagcatc agcgtttcta cccggggtgt tcaatatcac
     7321 cactcccttt tcgttggctg catcaatatc cacgttgatc actgttccgc gaaaatctac
     7381 aattgcaaat aaattttctg cttttcgaat catttcagcg gtaattgtgt cggattcggt
     7441 tacgacaaga tgacaccctt caattctttt ctcgaattcc tgtgtagaaa gcatccctct
     7501 ggcaaaccaa tcgtataatt caaggtcgga aaattgtttt tttaattcca ccagccactg
     7561 atctgtgatt cttgacgaaa tgaaagcttt catggtatca cctctatccg ttcgcttttt
     7621 cttggattaa tttctctgca ttccaatgcg ttaataaatt ccactcttct tgtgtgatca
     7681 atttatcctt tgaattgggg aatcgagacg ttttaaaaat gttttccaga tcgttcagcg
     7741 tatttgttgg atcgaagact ggaaaatcac tggcgaaaag caattttttt attccgtccg
     7801 cataaacctt tatccaggtt aatacctgct ccagcaaata cgttccaccc gttcgtcctg
     7861 aattggcaca aatatccgca tatacgttgg tgtgctttct cagcaacagt gccgcgtcct
     7921 gataatacgg tctaccgaca tgtgcaatga tcatgcttag ctctggaaac cgaacggcga
     7981 tatcatccag caatattggg tggctgaatt tatcgctgtg gctagccagg ctggctcctg
     8041 tatgaaacaa gataggcacc tgtttttgtt gacaatattc ataaagagga aatagccgtt
     8101 cctggttggg ataaaattcc tgcatgctgg ggtgcagttt tacccctcga aaccctaatt
     8161 catcaaaaca acggtccaac tcctttaatg atcctgagac atcttgtgga ttgataatgc
     8221 cgtatccaat caatcgcccg ggatactgtt gtatatacat ggcagttaaa tcattcaatt
     8281 tttgccattg actgtcaggg ggcataaatg tcaaggggaa aataacgcct tgagagattt
     8341 ttccctcatc caatgttctg atgattcctt cgggagatat cttttctttt atatttttcc
     8401 aaatgttctt gcttttcatg aagctgacaa tatcagcgga aagaagactc tctttgccta
     8461 tatgcatatg tgtatcaatg atagtgttca tgcttctaaa atcctttccc catacatggt
     8521 tttataatgt tgtttccaat cagcgaacag ttcttgataa accgaacccg attcatattc
     8581 tggttgtaaa agaaggtctt cttttacaca aacctgcgct ccttcgatca tgcttttaaa
     8641 aatgtttgca cccacgcccg cacagattgc agcaccgagc gcactgcctt ccttttgctt
     8701 tggcacaaaa actggcacct gcagaagatt tgccatgatt tgcaacaaag ttctgctttt
     8761 cgtcaaacca ccacatgcgc gcaggtccaa cagagccagc ccggcaacgc gttgcagacg
     8821 atcgacgttc aatttgactc cgtatgccac tgcttccagc gcagcgcgtg agaaatcttt
     8881 acgagccact cgcccgggtg tggcgggcag ttgtgttaca aagccacccg aacaattgaa
     8941 gtcaagattt cgatagttgg gcaaacgagg accgatatat gctttcagac cgtttgagcc
     9001 aggcggagca gtggatgcct ctttttccat cagtgtgtac acatctccga tttcgttggc
     9061 ataaaagata tccctgatcc aacggaaggt ttgtccggct tccatgctgg tgctttctat
     9121 aacccaggca tggggtacca cgtggcaacc actccaggtt aattctggtt ttgcgatgac
     9181 cggattgttg agcaccattt gcaatggacc gtatgtgccg agcgttactc ctatttctcc
     9241 ctcagcaact gctccgtttc ccagcaaacc gcattgtgaa tccgcaccgc ctgaaacaac
     9301 agcagtgcct gcagacagac ctgtttctgc cgcagctttt gttgtaacct ttccaatgaa
     9361 tgttcccgat tcgtatacag gtgggaagta gcgctccgga aatcccagct tcttaatgag
     9421 aggggaattc caatctcgtt ttttcagatc aaccagcaga gtttctacac cgttggaagg
     9481 atcggttatt ctttctcccg ataagcggaa aagaatccag ttgtttaata agactattga
     9541 atcaattttt tcataaattt ccggtttatt ttctttaaac cacaacaaac ggtaaggcaa
     9601 gaacatcggc atgggccagt gtccgctaga tgcgtgaatg ttctctgcga actgggcttc
     9661 aaattcgctg gaattgcgtg gcgcacgcat atccaggttg ggccctgcat ataattcctt
     9721 cccgttttta tctaaaaaaa ccaccccctc acgttggctg gttgaactga cggcaatgat
     9781 ctgcgcagga tctattcccg cttttttaat ggttcgtctg atcaaattgg atagaatgtc
     9841 ccaaaattcc tctgcgttga attctgagcc ccccggttgg gcttcaggtg gataggaata
     9901 ttcccattct gaatactgct caaaactttg ttgaccatcg acggagatga gataacacct
     9961 gccagcgcct gtgccaaaat ctaatgccat gagaaattgt cttcttttca ccatgtttat
    10021 ctcctcatct atttgttctt tgaaatgatc ttctgcaatc cacttaccag aatgatggtg
    10081 atcaacaatg cccccttaat aatgcgcaga tattctggcg gcgttccaag aataaaaaga
    10141 ccgttgatga tcatatagag aaacaacgaa ctgaaaatcg ttccttgcac agaaaaacgg
    10201 ctaaagatgg catttcccaa aattggcaaa aggaatgcgt cgatctgata actgtcgccg
    10261 cttcctactt gagccattcc ttcgttaaag gtgagcataa ttccggtaac ggcacagcat
    10321 aagccacaaa ttacgtatgt gatccaggta tagaaacgga ttgggattcc ggagtatctc
    10381 gcggcgtgca tattggaacc aaccgttcta ataaaacgac cgtaaatcgt atgatccagg
    10441 aaaacgtata agaatacagc aaccattacg gctgcgctga tgaaaaaagc cggctttcca
    10501 ccgagtgcaa cagcatctcg cggcagcatt aaataagtgc ctgatactgt gctaagcgct
    10561 accaggtatt gtaagccgcg cgccaaatag atgacggata aggtaacaac gaagggacta
    10621 attccgcaat aggcaaccaa aaacccgttc aatgctccaa tcagtgcacc cgtgatcaga
    10681 gcaaccagga tcatcaatgc aatggacata tccagttttg cagatcccac cgcagcgacc
    10741 atgccggcta aaccggcaat ggatgcaacc gaaagatcaa ttccaccgcc tgacattacg
    10801 atggtcatac ctatggatat gactgccgag attgtggctg gcaggatcag atttcgaacg
    10861 atattcacct gggttaaaaa tcctggcgca ttgatgccaa agtacataaa aaccagtgcg
    10921 aaaactgcaa agccagccaa agttccgaag ttattttttc ttcctctagt aagagccttg
    10981 tttggggtag ccaatggtcc gttcatcgta gtttccttga aattacagtt ttatttgaat
    11041 aatgtcgcgt tttccaacgt ttgctaagaa aatggcaagg attaaaatga tcccgtttgc
    11101 aatagggact gcccaaaatg gtgagttcat cagaacaaag gcattgtcga tcattgccat
    11161 catcaaagct gcaagaaaga cccccggaat attcggtctt cccatcccta agaaagtctg
    11221 accgacaaaa gcgaccgtca tgacgggcat aagcatattt gctccagatg tggctaatgc
    11281 ccccgacatt tttgccgtgg aaaaaaatgc gccgatcgca aaaataaatc ctccaagaat
    11341 gaagcctgct gttttatacg ttattgaatt tacccccatt gttttggctg cttcgggatc
    11401 ttcaccaacc gctcgcaggc ggattccaaa cgatgtatgc tctgtgagaa agtaaaggat
    11461 gacaaaaagc aagagaaaga aaatcacaat cttgggaatg cctgctaagc ttccaaagat
    11521 gagatcgcga ttgaattttt ccttaaagta aacattttca ccgcctgtgt atgcatacca
    11581 gattccttgc aataaaaagt acatggaggt ggtagtgacg tacgatacca gtttcaatcg
    11641 tgtgaccaat tgagaaatta ccagtgaaat agtaatgcca gccaggaacg aaactccgag
    11701 agaaggccag acccccatat ctttttggag cagattcgcg aagatggcgc cttccagggg
    11761 aattgccccg gcatacgaaa gatcaatttc tcctgccatc ataatgattg ccagcccaaa
    11821 accgacaatg ccgagtatag tcgattgaac cagcaggttt agcagattgt caactttcaa
    11881 aaatgatggt ttcaaaaatg cgaatgtcaa aaataaaatg ataagaatgc caaagactgc
    11941 attttttcga acggcatttt tagcggtttt catcaggatg acctcccatt gcaaacatgg
    12001 atacttcttc tattgaagtt ttcagcggat ccaattctgt aaggattgac cctttgtaca
    12061 tcaccgcaat gcggtcgcaa acctgtaacc cctcttccac atcgctggtt acgaagagca
    12121 caccagcacc attgtcggct aattcgtgca tcaggttata tatctccgtt tttccctgga
    12181 catctacgcc aaccgtgggc tcatcaaaga taaagatctt gcttgttgta cataaccatt
    12241 tggcgagcac gactttttgt tggttccctc cactcaattc ccgtgtgtgc ttttcaatcg
    12301 aaggagtggc aatatgtagc cggtggacca gtttgataac aatatcgcgt tctttttgaa
    12361 cgcttgtgaa aaacctgctg gttgtaaaat ttctcaatcc tgccagagtg atgttgcttt
    12421 gaatagaatt gtcgagcagc aaaccgtgat actttcgat
//
LOCUS       DAOSDS010000059        12206 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2009_length_12206_cov_3.927496, whole genome shotgun sequence.
ACCESSION   DAOSDS010000059 DAOSDS010000000
VERSION     DAOSDS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12206
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2009_length_12206_cov_3.927496"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLL88_07685"
     CDS             <1..364
                     /locus_tag="PLL88_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01482.1"
                     /translation="AKGGADVVIEFSAAKSGFELANRLFKQAGKLVIGSWHRTPMEFD
                     GTRWHLGGVTVLNLSPMANAHYTEMLPRTYELIKRGVYEPGKLVTHVANYKDCKEVFL
                     RAIDKKDGYMKGVILFND"
     gene            505..900
                     /gene="rbsD"
                     /locus_tag="PLL88_07690"
     CDS             505..900
                     /gene="rbsD"
                     /locus_tag="PLL88_07690"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HOJ01483.1"
                     /translation="MKKEKLLNPEIVAAVAALGHTEYLCIADCGLPIPQGVTVIDVSV
                     CATIPSFLQVLKAVEGELVVESYIIADEIKEANPNILKQMEEILAGRSAKKVPHEEFK
                     KLTSNAKYIIRTGETSPYANVILVGGVNF"
     gene            919..1818
                     /locus_tag="PLL88_07695"
     CDS             919..1818
                     /locus_tag="PLL88_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01484.1"
                     /translation="MSNSFPRTMVGDVSMPRLIIGCNWVSGFSHLSAAGDTMIRQTHC
                     GPQSVSSIFETFLKHDIDAVLGLFDVDKNLLPAVNLAQERSGKKMIIIDEPILNMDDS
                     PAARLEAKKKIDECAQRGATFCMPLHSCVEQLVNKNTMTMDRLPDYLYMIREAGMIPG
                     LSAHMPEVVQYADLNEYDVETYIQIYNCLGFLMQVEIESVAKVIQNAKKPVITIKPLA
                     AGRTTPFVGLNFVYNTIREQDMVAIGCFDANQADEDIEISRAAIERRAPQISNRNSPL
                     ETSVITGVIEDSFSADSSLKKGG"
     gene            1887..2861
                     /locus_tag="PLL88_07700"
     CDS             1887..2861
                     /locus_tag="PLL88_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ01485.1"
                     /translation="MNKKLYLVLSFILVATFALTACAPKEAAPAEEAAPVEETSSKPL
                     KIGFSLPTLQFPFYVRMHDSFVAEAEARGWEVVFVDGNLDSLTQVNATLDLLTQDIDA
                     LVMATWWIDAMADVFDIAEKSDIPVFLMDHSAAAIPEGFTYASAAGTDNYDAGYVGGV
                     WMSERLKAEDKSSVNLVLVTMTSETAVKRCDGFVAGMADNGIEVTVLNEYLGDTRANG
                     MRSMEDALITYPAGQIDLVFGYSAQSSLGAYDAIVAANRTEIQVVGFDGEDDEIALID
                     AGTQYIGTVVQYPDQMAILTAQLVEKVVINGETIEQAQPVAAGVYDGK"
     gene            2975..4468
                     /gene="gguA"
                     /locus_tag="PLL88_07705"
     CDS             2975..4468
                     /gene="gguA"
                     /locus_tag="PLL88_07705"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOJ01486.1"
                     /translation="MQGDEHILTLSNVCKEFPGVKALSNVDFALRKGEVHALVGENGA
                     GKSTLMKILSGAYTKDSGTIVFNGKEVEIASPKAAEKMGISIIYQELNLINRISVAEN
                     VFLGRAPRKNGVIQWNKMYKDAKQLFDSFDIEMDVSKYARDFSIAQQQIIEIIKAVSI
                     NAKVVIMDEPTSSLTDNETQILFRIIRSLKSKGISIIFITHRLDEVFEISDRLTVLRD
                     GCFIGTKDIKDINKSELIKMMIGRELKQQFPQRDVQIGEECFRVDHISDGKKIKDVSF
                     SVRKGEVLGFAGLVGAGRTETMRLIFGADKKTSGKIYINHREVQIKRPKQAVGKRVGF
                     VTENRKEEGLFLRLPVRNNIVAVAIEKIKSMKILNFKKEKFFADKYVKELRINTPSIN
                     QSVVFLSGGNQQKVVLSKWLLSDSEVMILDEPTRGIDVGAKREIFEIINHLAAQGKAI
                     VFISSEMEEILGISDRILVMHEGKISGEVTKENFSERIITEYAFGGA"
     gene            4470..5450
                     /locus_tag="PLL88_07710"
     CDS             4470..5450
                     /locus_tag="PLL88_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01487.1"
                     /translation="MQTKAMNKRLGSLTHEFNKLMNAMGVWFVVILFFVILSLTTDTF
                     LSYANLINVIRQICVNAMVALGASYVILGGEIDLSTGNMCAFAGCGAALLMVAGVNMW
                     LAIAIILVVGAIIGCLTGAVVTFIKIPSFIASLGMSYVILGANLLLTNSEPISGLPAQ
                     FMTLGRGYIADVIPVPVIILIIFFTIGAFFLKYTSFGRSVISVGENPIAARLSGIDIL
                     TTKVLIFAIGGFTAAAAGVVLASRLSSGQPMAGGDIGLTAIAAVFVGGTEKGSALNVL
                     AGAFVLGLVNNGLNLLEVNAYWQKVALGLIIVLAVLLDQIRASRAVSIKK"
     gene            5472..6215
                     /locus_tag="PLL88_07715"
     CDS             5472..6215
                     /locus_tag="PLL88_07715"
                     /inference="COORDINATES: protein motif:HMM:NF018038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HOJ01488.1"
                     /translation="MKQKALIFYGGWSGHQPELVSQRVKKMLEKEGFEVTLSDTLECL
                     ADKDALKELDLIVPMWTQGHMDDRYAFHISEAVESGVGIGGNHGGMCDSFRWNIEYQF
                     ITGSQWVSHPGDTWYHHISDLSKENLDYVQKYYPSPEGAFETDYVVNIKRGVASPIIE
                     GIQDFSVHSEQYYLHIDPCVNVLATTQVQTVGPHSANGVVTMPVVYTKLWGKGRVFYC
                     SVGHVDKIYEIPEVSEITRRGLLWAARKR"
     gene            6331..7770
                     /locus_tag="PLL88_07720"
     CDS             6331..7770
                     /locus_tag="PLL88_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HOJ01489.1"
                     /translation="MKVMIGAFAHESNDFCPQKTTREMFEFYEGKEVISHLPVCDIFE
                     EAGLEVIPSIYADAQSLGPVEEETYLYFENKILDIVRANPDLDGIWMFCHGAMNVENI
                     GSGEYRLASDLRKIVGEDCVIAFGMDLHGNIEADFAEKVNIFRCYHTAPHTDQVDTYR
                     RTAKALLHYLKNGYKTHCKMRKIPMIFPGEMAATTVDPFKGIIQTTKEWEENDPRILC
                     ASTFIGFAWTDAERTSSTAVVVPTAPEHEEYCERKAEELAALAFSKRGEFDFEMLSLP
                     PAETARTSLYELEAPVCVTDMGDNPTAGTTGGSNVLLKEYLKIHDPNKKILIVGILDK
                     AAFDTCKKHRVGDVFELTIGMDIDEVTCPVTVQARYKGCHDILGYTIPSIPPIKRCEG
                     VTVAIGNIDVVITDKAYAFTQEVNFNSCELDPGTYDVFVTKFGYIFPELKHMGKDFIM
                     SNTPGESYQMIKSFDYKKISRPIYPIDEI"
     gene            7927..8313
                     /locus_tag="PLL88_07725"
     CDS             7927..8313
                     /locus_tag="PLL88_07725"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HOJ01490.1"
                     /translation="MGFKLSQNVSLQLFYKGKVVFQSKSHWIMPLFELEDHLKEHPVD
                     LALAEVHDKVVGKAAAMLILRLGIGRVFGNVMSELADATLSQAEVPHFYHELVDRIDC
                     KTEELLLEVDDKDAAYEMLCKLARRC"
     gene            8425..9192
                     /locus_tag="PLL88_07730"
     CDS             8425..9192
                     /locus_tag="PLL88_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOJ01491.1"
                     /translation="MDTREAIREKVWNALRKVALPDSRFHYDFSEFIADFAGSDQATA
                     RLCDMDIYRNAKVIFITPDNCLEQLRSQTIKDKKVLLTTTYGIRRGFVELLPEQVPTG
                     MEKYAILLDAVEKIGTYISLADIQKRYRVDLLVTGGSAVTRDGVRAGKGHGFFDIEWA
                     TLYSLGVANISTPIVDVVHDCQIVDEEFDLQPFDTLCDYIVTPTQVIHVPAPQKPTGG
                     IYWDRLEAGMMEQISILSELKELRDQGKLWQDGARGP"
     gene            9481..11400
                     /locus_tag="PLL88_07735"
     CDS             9481..11400
                     /locus_tag="PLL88_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01492.1"
                     /translation="MHGSGWRSYIRSGDEKPKFTWGLFKRVMQYGQAYGWHLLGMLVL
                     ILISSGLSLISPLVVRNLIDTTIPAKDVKMLIMLSIGLLAIPILKGVCSVLQRKLNTT
                     VGEGVIFDLRYALYEKLQRMSLRFFTHTRIGELMSRLNNDVMGAQNAISNTIVNMVTD
                     VIQAVAVISMMVSIEWRLTLISIAVVPLFILAARILGEKLRDVARQQMDGNARMNAMM
                     NETLNIGGVLLVKLFGRQGTEAQRFAQRASVVRDLGVKRAVIGSIFFMIISLFGAVGS
                     ALVYGYGGFQAIVGNFTVGTIVAFGSYLGMLYASLQSLANAPVEFSTSVVSFERVFEV
                     LDLPVEIAEKPDAKEISAVSGEIRFEHVHFQYDDSADHLLSDVRRFGQLSDDTAVLSD
                     EKAEEVQVEGAQEETQARDAALHNIHFTIPAGNLFALVGPSGAGKTTLTYLIPRLYDP
                     TKGRILLDGYDLRELQLASLVAQIGMVTQETYLFHDTIYTNLLYANESATQAEIEEAA
                     KAANIHNFIAQLPEKYNTIVGERGYRLSGGEKQRIALARVILKDPRILILDEATSSLD
                     SESEILIQEALSRVMAGRTSIVIAHRLSTILAADQILVLNRGRVVERGKHTELLQLDG
                     IYAGLYEKQFKKAGK"
     gene            11606..>12206
                     /locus_tag="PLL88_07740"
     CDS             11606..>12206
                     /locus_tag="PLL88_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01493.1"
                     /translation="MDDLAIQACSLSKVFKTKVGLWKIKSKSTTAVEDLSFEVERGEL
                     FGLLGPNGAGKTTTVKMLTTLLLPSRGTAKILGLDVIEQTAEVRRHIGFAFSGSRGLY
                     NRLTAIQNLNYFAELYALEPQVTKKRIQELLELVGLKGRGDDRVETYSSGMVQRLHLA
                     RALLHDPEVIFLDEPTVGIDPVGSRELRQTIKNLISLKKT"
BASE COUNT         3297 a         2653 c         3252 g         3004 t
ORIGIN      
        1 cgccaaaggt ggcgcagatg tggttattga atttagcgcg gcgaaatcgg gttttgaatt
       61 agccaatcga ttgttcaaac aggcaggaaa actggtgatc ggtagctggc atcgaacccc
      121 catggaattc gatggaactc gttggcattt gggaggcgta acggttctca atctttctcc
      181 catggcaaat gcccattaca ccgagatgct gcctcgcacc tatgaactga tcaaacgcgg
      241 tgtttatgag ccaggcaagc ttgtcacgca tgtagccaat tacaaggatt gcaaggaggt
      301 tttcctgcgc gcgatcgata aaaaagacgg ctatatgaaa ggtgtcattc tgttcaacga
      361 ttagatagag cgaaaacttc caccataaag aatatggtgg aagtttcgat ttggctgcaa
      421 gttcgcaacg aattatgagc acgtgcgtca tcgtgcatgg ctttgtttat tgataaaaac
      481 gaacggactt ttaacggaaa caacatgaaa aaagaaaaac tcttgaaccc cgaaattgtt
      541 gcagcagtgg cagcccttgg gcacactgag tacctgtgca ttgccgattg cggattaccc
      601 atcccacaag gagtgactgt aattgatgtg tcggtgtgcg ccacaattcc aagttttcta
      661 caggtgctga aggcggtcga aggcgagctg gtggtcgagt cgtacatcat cgcagacgag
      721 atcaaagaag ccaatccaaa catactgaag caaatggaag agatcctggc aggcagatct
      781 gccaaaaaag taccccatga agaattcaag aaacttactt ccaatgccaa atatatcatt
      841 cgaacgggtg aaacctcgcc atatgcaaat gtcattctgg tgggtggggt taatttctag
      901 tcaggaagga acgagtcttt gagtaattca tttcctcgca caatggtcgg cgatgtttcg
      961 atgccgagat tgatcattgg atgcaactgg gtatccggat tcagccatct cagcgcggct
     1021 ggcgatacga tgatacgcca gacacattgc ggtcctcaat cggtcagctc aatatttgag
     1081 acctttctga aacacgatat tgatgcggtg cttggtcttt ttgacgtgga taagaacctg
     1141 ttgccagcgg tgaatctggc gcaggaacgt agcggtaaaa agatgatcat cattgatgag
     1201 ccaattttga atatggatga ttcaccagcg gcgcgccttg aagcgaaaaa gaaaatcgat
     1261 gaatgtgcgc agcgcggagc gacattctgc atgccgcttc attcctgtgt ggaacaattg
     1321 gtcaataaaa ataccatgac catggatcgc ttgcccgatt acctgtatat gatccgggaa
     1381 gcggggatga taccgggtct ttctgcccac atgcccgaag tggtgcagta cgctgacctt
     1441 aatgaatatg atgtggaaac atatattcaa atctacaact gtctgggatt tttgatgcag
     1501 gttgaaatcg aaagcgttgc caaggtcatc caaaacgcca agaagcctgt catcacgatc
     1561 aaaccacttg ctgcgggtag aaccacgccg tttgtgggac tcaattttgt ttacaacacg
     1621 atcagagaac aggatatggt ggcgatcggg tgttttgatg ccaatcaggc agatgaagat
     1681 atcgaaatct cgcgcgcagc catcgaacgc cgtgccccac aaatatcgaa tagaaatagt
     1741 ccccttgaaa cgtcggtaat cacaggtgta attgaggatt cattcagcgc ggatagttcc
     1801 ctcaagaaag gaggatgatc gaggaaaaat tccctttacc agtacagaca aattattcgg
     1861 tacaaacaaa aaacaggaga agattgatga acaagaaact atatttggta ctctctttca
     1921 ttctggtggc gacattcgcg ttaactgcat gcgctccaaa ggaagcagca cccgccgagg
     1981 aggcagctcc agtcgaagaa acctccagca aaccattaaa aattggcttt tcccttccta
     2041 cactccagtt tcctttctat gtaagaatgc atgattcctt cgtggcagaa gctgaagctc
     2101 gtggttggga agttgtattc gtcgatggga acctggattc tttgacccag gtgaacgcga
     2161 ccctcgatct gttaacccag gatatcgatg cgttggtgat ggcaacctgg tggattgatg
     2221 caatggctga tgtatttgat attgccgaaa agagtgatat tcccgtgttc ttgatggatc
     2281 actctgcagc cgcaattcca gaaggtttta cctatgcttc ggcagcaggc acagataact
     2341 atgatgccgg ctatgttggt ggtgtgtgga tgtcagaacg tttgaaagct gaggacaaat
     2401 ctagcgtaaa cctcgttctg gtcaccatga ccagcgaaac agccgttaaa cgctgcgacg
     2461 gctttgttgc cggtatggct gataatggca tcgaagttac tgtcttgaat gagtaccttg
     2521 gtgatacccg cgccaacggc atgcgctcta tggaagatgc attgatcact tatccagcag
     2581 gacaaattga cctcgtcttc ggatacagtg cacaatccag cctgggcgct tatgatgcca
     2641 ttgtggctgc caaccgcaca gagatccagg tggttggatt cgatggcgaa gatgacgaaa
     2701 ttgctctgat cgatgccggc acgcaatata tcggcactgt tgttcagtat cccgatcaaa
     2761 tggcgatcct cacagcacag ttggttgaaa aagtggttat caatggtgaa accattgaac
     2821 aagcccaacc ggttgccgct ggcgtgtacg acggcaaata agctgctgaa aaatttgaga
     2881 tttcagactt aatattgttt atgcctgaca gagcaacttt tattggtaat ccgctctgtc
     2941 aggcaaaaca cttgagggtg tacggaggaa gaagatgcaa ggtgatgaac acatactgac
     3001 ccttagcaat gtctgtaaag aatttccggg tgtcaaggca ttaagcaatg tggattttgc
     3061 cctacgaaaa ggagaagtgc atgccctggt tggggaaaat ggggcaggaa aatcaacact
     3121 gatgaaaatt ctttctggcg catatacaaa agacagcgga acgattgtat tcaacgggaa
     3181 agaagtggag attgcatctc ccaaggcggc ggagaagatg ggtatctcaa tcatctacca
     3241 ggaattgaac ctgatcaacc gcatcagtgt agctgaaaat gtattcctgg gtcgagcgcc
     3301 aaggaagaat ggtgtgatcc agtggaacaa aatgtataag gatgcaaagc aattattcga
     3361 tagcttcgac attgaaatgg atgtgtcgaa gtatgcgcgt gatttcagta tcgcacagca
     3421 acagatcatc gagatcatca aagcggtgag catcaacgca aaagtggtga tcatggatga
     3481 acctacttct tcattgacag acaatgaaac acaaatattg ttcagaatca tccgaagcct
     3541 gaagagcaaa ggcatttcga tcattttcat cacccaccga ttggacgaag tgttcgagat
     3601 ttcagatcga ttaaccgttc tgcgcgatgg ttgttttatt ggcacgaaag atattaaaga
     3661 tatcaacaaa agtgaactga tcaaaatgat gatcggtcgt gaattaaaac agcaatttcc
     3721 acaaagagat gtccagatag gcgaagaatg ctttcgggtt gatcatattt ctgatggaaa
     3781 aaagatcaag gatgtttctt tcagtgttcg aaaaggcgaa gtacttggat tcgcagggct
     3841 ggtaggtgcg ggacgtaccg agaccatgcg cttgattttt ggagcagaca agaagaccag
     3901 cggcaagatc tacatcaatc atcgagaggt tcaaatcaaa cgcccaaaac aagctgtggg
     3961 aaagagggtc ggattcgtta cagagaaccg gaaagaggaa ggattatttt taagattgcc
     4021 tgtacgaaat aatatcgttg cggtggcaat cgaaaaaatc aaatccatga aaattctgaa
     4081 ctttaaaaag gaaaagtttt ttgccgataa atatgtgaaa gagttgagga tcaatacccc
     4141 atccatcaac caatcggtag ttttcctaag cggcggaaac cagcaaaagg tggtgctgtc
     4201 gaaatggtta ttatcggatt ccgaggtgat gatcttggat gagccaactc ggggtatcga
     4261 tgttggtgcg aagcgcgaga tcttcgaaat catcaaccac ttggcggcgc agggaaaagc
     4321 cattgttttc atctcttcgg aaatggaaga gatccttggt atcagtgatc gcatcctggt
     4381 catgcacgaa ggcaagatct cgggtgaagt caccaaagaa aatttttcag aacgtatcat
     4441 taccgaatac gcgtttggag gagcatagca tgcagaccaa agcaatgaat aaaaggctgg
     4501 gttctctaac ccatgaattc aacaaactca tgaatgccat gggcgtgtgg tttgtggtga
     4561 ttctattttt tgtcatcctt tctttgacca cggatacatt tttatcctat gccaacctga
     4621 tcaatgtgat caggcaaatc tgtgtgaatg ccatggtggc attgggagcc tcttatgtca
     4681 ttctgggcgg tgaaattgac ctcagcaccg gcaatatgtg cgctttcgca ggatgcggag
     4741 cggcattgct gatggtagca ggagtgaaca tgtggctggc gattgcgatc atcctggtgg
     4801 ttggagcgat cattggttgc ctgacaggcg cggtggttac atttattaag ataccttcct
     4861 ttatcgccag tttgggcatg tcgtatgtca ttttgggtgc aaacttactc ttaaccaaca
     4921 gcgaaccgat ctctggtttg ccggcacaat tcatgaccct gggcagaggg tatattgcag
     4981 atgtcatccc ggtaccggtc atcattttga tcatcttctt cacgatcggc gcatttttct
     5041 tgaaatatac ctcttttgga cgaagcgtca tatcggttgg tgaaaatccg atcgcagcgc
     5101 gcttatctgg tatcgatata ttgaccacca aagttctgat ctttgcgatt ggtggtttta
     5161 ccgctgctgc agcgggggtg gtactcgcct cgcgcttgtc gtctggtcaa cctatggcag
     5221 gcggtgatat tggtctgacc gccatcgccg ccgtgttcgt gggcggcaca gaaaaaggga
     5281 gtgctttgaa cgttttggcg ggtgcgttcg tgctggggtt ggtaaacaac ggtctaaacc
     5341 tgctggaagt gaacgcttac tggcaaaaag ttgccctggg tttgatcatc gtcctggcag
     5401 tgctgcttga ccagattcga gcaagccggg cagtttcaat caaaaaataa tacaggtaca
     5461 aggagaaaaa catgaaacag aaagcattga tattctatgg tggctggtct gggcatcaac
     5521 cggaattggt cagtcaacgt gttaaaaaaa tgctggaaaa agaagggttt gaggtgacct
     5581 tgagcgatac ccttgagtgc cttgcagata aggacgcctt gaaggagctg gacttgatcg
     5641 ttcccatgtg gacgcaagga cacatggatg accgctatgc tttccacatt tccgaggccg
     5701 ttgagtcggg tgtaggcatt ggcggaaacc acggcggtat gtgcgattcg ttccgatgga
     5761 acattgaata ccaattcatt accggcagcc aatgggtatc tcatcccggc gatacatggt
     5821 atcaccacat ttcagacctc tccaaagaga atctggatta tgtgcagaag tactatcctt
     5881 cacctgaagg cgcatttgag acagattatg ttgtcaatat caaacgcggg gtggcatccc
     5941 ccatcattga aggtatccag gatttcagcg ttcattccga acaatactat ttgcacattg
     6001 acccatgtgt gaacgtgctt gccacaaccc aggtacagac ggtgggtcca cacagtgcca
     6061 atggtgttgt gacgatgcca gtggtctata caaaactttg gggaaaggga agagttttct
     6121 attgctccgt cgggcatgtt gacaaaatct atgagattcc ggaagtatcc gaaatcaccc
     6181 ggcgcgggct attgtgggct gccagaaagc gctgatagcg ttaaaaggaa tagaacaaac
     6241 ggtaaaaatt gcggcaaagt acgagcatac tctgtcgcaa ttttcttaaa ttcgatcttg
     6301 aggtgttagc agtatggaaa ggaggaaaac atgaaggtga tgatcggcgc atttgcgcat
     6361 gaatccaacg atttttgtcc ccaaaaaacc acacgcgaga tgttcgaatt ctatgaaggg
     6421 aaagaggtga tatcccatct gccagtatgc gatattttcg aagaagccgg gcttgaggtt
     6481 atcccttcga tctatgctga tgcccaatca ctgggacccg tggaagagga gacttatcta
     6541 tattttgaaa ataagatcct ggatatcgtg cgcgctaacc cggatttgga tggcatctgg
     6601 atgttttgcc atggcgctat gaacgtggag aacattggaa gtggtgaata tcgattggca
     6661 agcgacctgc ggaagattgt tggggaagac tgcgtgatcg catttggcat ggatctgcat
     6721 ggaaacatcg aagccgattt tgcagagaag gtgaatatct tccgctgtta tcacaccgcc
     6781 ccgcataccg atcaagttga cacttaccgg cgcactgcca aagctctgct ccattacttg
     6841 aagaatggat ataagaccca ctgcaaaatg cgcaaaatcc ccatgatttt tccgggggag
     6901 atggcagcca caactgtaga cccctttaaa gggatcattc aaaccacaaa ggaatgggaa
     6961 gagaacgacc ccaggattct gtgtgccagt accttcatcg ggttcgcctg gaccgatgca
     7021 gaacggacct cttccacggc ggtcgtcgtc cctacagcac cggaacatga agagtattgt
     7081 gaacgaaaag cggaggagct ggcggcattg gctttttcga aacgaggaga attcgatttc
     7141 gagatgctca gccttcctcc ggctgagact gcgcgaacct cactctatga gttggaagct
     7201 cctgtatgcg tgaccgatat gggcgataac cccaccgctg gaacaacggg gggtagcaat
     7261 gtattgttga aggaatacct gaagatccat gatccgaaca aaaagatatt gattgtggga
     7321 atactggata aagcggcttt tgacacctgc aaaaaacacc gcgtggggga tgtctttgaa
     7381 ctgaccatcg gcatggatat cgatgaggtg acctgcccgg tcaccgtgca ggcaaggtac
     7441 aaaggttgcc atgacattct ggggtacacg attccttcca tcccacccat caaacgttgt
     7501 gaaggtgtga cggttgcgat cggaaatatc gatgtagtga tcaccgacaa agcgtacgct
     7561 ttcacccagg aggtcaactt taactcttgc gaactcgacc ctggaaccta tgacgtgttt
     7621 gtgacgaaat ttggttatat tttccccgag cttaaacaca tggggaaaga tttcatcatg
     7681 tccaataccc ccggtgaaag ctaccagatg atcaaatcgt tcgattataa aaagataagc
     7741 cgtccgattt acccgattga tgagatctga cggtttcttt tcttttgggg attgcttgta
     7801 ttcattttcc ggtctgcggt aatggaatag ccacagcaaa gattgcgggc tggtatgtta
     7861 caattttacg gttttgcttc aaatatgcga atatatttga gaatggttta agcggagaag
     7921 aagatcatgg gtttcaagct ttcacaaaat gtctctttac agcttttcta taaaggaaag
     7981 gttgtttttc agagcaagag tcattggatc atgccattgt tcgaacttga ggatcacctg
     8041 aaggagcatc ccgttgattt ggcgctggcg gaagtgcacg ataaagtggt cgggaaagcc
     8101 gcggcgatgc ttatcctgcg cttggggatc ggccgcgtgt tcggaaatgt gatgagcgaa
     8161 ttggcagatg ccacgctttc ccaggcggag gttccccact tctatcatga gctggtagat
     8221 cggattgact gcaaaacgga agagcttttg ttggaggtag acgataaaga tgctgcctat
     8281 gagatgctgt gtaaacttgc caggcgctgt taagaatgat tgctgtggaa accctgatcg
     8341 gactccacag caattttttc tatttgtacg agactttgaa aacaggcgat accattggga
     8401 gagcgttgct ggaggagaac ttgtatggac actcgcgaag ccatcagaga gaaagtatgg
     8461 aacgcattac gcaaggttgc attgccggat agcagattcc attatgattt cagcgaattc
     8521 attgctgatt tcgcaggcag tgaccaggca actgcaagac tatgtgacat ggacatctat
     8581 cgaaatgcta aagtcatttt tatcacccct gataactgtc tggaacaatt gcgcagccag
     8641 acgatcaagg ataagaaggt tctattgaca accacctatg gcattcgacg gggtttcgtg
     8701 gagttactgc cggagcaagt tccgacggga atggaaaaat atgccattct ccttgatgcc
     8761 gttgagaaga tcggaaccta tatttccctg gcggatatac aaaaacgtta ccgggtagac
     8821 ctgctggtca ccggtggatc agcggtgacg cgggatgggg tgcgcgccgg caaagggcac
     8881 ggttttttcg atatcgaatg ggcaacgctc tattctctcg gagtggcaaa tatcagcacg
     8941 ccgatcgtgg acgtggtgca tgattgccag atcgtggatg aagaatttga cctgcaacct
     9001 tttgacacgc tctgcgatta tatcgtcacc ccaacgcagg tgattcatgt gcctgctccg
     9061 caaaaaccga caggaggtat ctattgggac cggttggaag cgggcatgat ggaacagatt
     9121 tcgattctca gtgagttaaa ggagctgcgt gaccagggaa aactgtggca agatggggcg
     9181 cgtggcccgt gaacgtccca tacggttctt cctagcattc ccattccccg gcatctgatg
     9241 ccgggcgcca tacccgcaaa ttagccttgc catcaagacg aacaacacag caacttgctg
     9301 catagcgtgt gatcggctgt cttttctacg cttgcacgct tgggttacag tcacattctc
     9361 tccgattttc tcatctttca tgatatgtct ggggaggcaa agcgtataca ataagagcaa
     9421 aatatatttg catttttcat gctagattta acataaatcc aaacaggaag gtcaatcact
     9481 atgcacggaa gcggatggcg ttcgtatatt cgatcggggg acgagaaacc aaaattcacc
     9541 tggggactct tcaagcgcgt gatgcaatat gggcaggcat atggctggca cttgctgggc
     9601 atgctggtgc tgatcttgat cagttcgggg ttgtcattga tttccccact ggtggtgcgc
     9661 aacctgatcg acaccaccat ccccgcgaaa gatgtgaaga tgctgataat gctctccatt
     9721 gggttgctgg cgatcccgat cttgaaggga gtgtgcagcg ttctgcagcg caaactgaat
     9781 acgactgtgg gggaaggggt gatcttcgat ctgcgctatg cgctgtacga aaaactgcaa
     9841 cgcatgtcgc tgcgtttttt cacccacacg cgcattggag aattgatgag ccggctgaac
     9901 aacgatgtga tgggagcgca aaacgcaatc agcaatacga ttgtaaacat ggtgacggat
     9961 gttattcaag ctgtggcggt catcagcatg atggtgagca ttgaatggcg cctgacgctg
    10021 atcagcattg ctgtggtgcc attgttcatc ctggcggcgc gcatattggg agaaaaactg
    10081 cgagatgtcg cccgccagca aatggatggc aacgcgcgca tgaatgccat gatgaacgaa
    10141 acactcaaca tcggtggcgt gttgctggtg aagctgttcg ggaggcaagg tacggaagca
    10201 caacgttttg cgcagcgcgc atctgtggtg cgcgacctgg gcgtgaaacg ggcggtgatc
    10261 ggttcgatct tttttatgat catcagtttg tttggagcag tgggcagcgc tttggtatat
    10321 gggtacggcg gttttcaagc catcgtaggg aatttcaccg taggcaccat cgtggcattc
    10381 gggtcttatt tggggatgct gtacgcctcg ctgcagagcc tggcaaacgc gccggtggaa
    10441 ttcagcacct cggtagtgag ctttgagcgc gtgtttgaag tgctggactt gccggtggag
    10501 atcgctgaaa agccggacgc aaaggagatc agcgcggttt ccggggagat ccgcttcgaa
    10561 catgtgcatt tccaatatga cgacagcgct gaccatttat tgagcgatgt gcgacgcttc
    10621 ggtcaattga gcgatgacac agcggtgctt tctgatgaaa aggctgagga agtccaggta
    10681 gaaggtgcgc aggaggaaac gcaggcgcgc gatgcagcgt tgcataatat tcatttcacc
    10741 atcccggcgg gaaatctatt cgcgctggtg ggacccagcg gagctggcaa gaccactttg
    10801 acctatttaa tcccacggct gtatgatccg accaaaggtc gcatcctgtt ggatggatat
    10861 gacctgcggg agctgcagtt ggcgtctttg gtcgcacaga tcggcatggt gacgcaggaa
    10921 acgtacctct tccacgacac catctatacc aacttgctgt acgcaaatga aagtgccaca
    10981 caggcagaga tcgaagaagc cgcgaaggca gccaacattc ataatttcat cgcacagctt
    11041 ccagaaaaat acaacaccat cgtgggtgag cgcggctatc gtttgagcgg cggagaaaaa
    11101 cagcgcatcg cattggcgcg ggtgatccta aaagacccgc gcatcctcat tctggatgaa
    11161 gccaccagca gcctggacag cgagtcggaa attctcatcc aggaggcatt gagcagggtg
    11221 atggcagggc gcaccagtat cgtgatcgcg caccgcctga gcaccatttt ggcggcagat
    11281 cagattttgg tattaaaccg cgggcgcgtt gtggaaaggg ggaaacatac cgagttactg
    11341 caattggacg gaatttatgc cgggttgtat gagaaacagt ttaaaaaagc cggcaagtaa
    11401 gaggaaaata aatttccact gaatccacca atttgtaaaa ataagggcgc aggactactg
    11461 aagattttgc gtgatcgaca agccatccgt gaatcagagg taaaattgca cctgttgacg
    11521 ttttgcataa catatttttt tatagcaatt ccaacaaatt ttcttcaaaa aaatgatcaa
    11581 tcaattgcac ggttaacgag ggaaaatgga tgatctggct atccaggcat gttcactgag
    11641 caaagtcttc aaaaccaagg tcgggttgtg gaagatcaaa tccaagtcca ccacggcagt
    11701 ggaggatctt tcctttgaag tggaaagagg agaattgttc ggcttgctgg gtccgaatgg
    11761 ggcaggcaag accaccaccg tgaaaatgct gaccacgtta ctgttgccaa gtcggggaac
    11821 agccaaaata ttggggttgg atgtgatcga acaaaccgcc gaagtgcgca ggcacatcgg
    11881 ttttgctttc agcggtagcc gcgggttgta caaccgcctg accgccatcc agaatctgaa
    11941 ctattttgcg gaattgtatg cgcttgaacc gcaggtgaca aaaaaacgca tccaggaact
    12001 gcttgagctg gtggggttga aggggcgggg ggatgaccgc gtagaaacct attccagcgg
    12061 gatggtgcag cgcctgcacc tggcacgcgc gctgctgcac gatcccgaag tgatattcct
    12121 ggatgagccc accgtaggca tcgacccggt tggatcgcgt gaactgcgcc agactatcaa
    12181 gaacctgatc tccttaaaaa agacca
//
LOCUS       DAOSDS010000060        12205 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2010_length_12205_cov_4.005926, whole genome shotgun sequence.
ACCESSION   DAOSDS010000060 DAOSDS010000000
VERSION     DAOSDS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12205
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2010_length_12205_cov_4.005926"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLL88_07745"
     CDS             <1..644
                     /locus_tag="PLL88_07745"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOJ01494.1"
                     /translation="FGINGSGMHCHQSIFDKSGNNLFFDAKDEFHLSKLAYGFIAGQL
                     SHAKALSAVVAPTVNSYKRLVPGYEAPVYIGWAQTNRSALIRIPRYTIGKDKSMRAEL
                     RFPDPSSNPYLAFSAMIAAAVDGIEKGLTPPKPLNNVNIYDLSREERITRGIESLPGS
                     LKEALVELAADPIVKDSIGAKLYAAFYRAKEEEWENYRIHVTDWEIENYLQSA"
     gene            complement(688..1608)
                     /locus_tag="PLL88_07750"
     CDS             complement(688..1608)
                     /locus_tag="PLL88_07750"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ01495.1"
                     /translation="MKCTKCGQKAVMHMEHHNLALCQDHFPDWMRTQTAHIIKKYSMF
                     RHEERLLLAVSGGKDSLALWDILWKLGYQVDGIYLDLGINGETNYSAISGETAQTFAN
                     ERGLRLTTINVKARYGESIPELGSRTKRGHQKPCSQCGLVKRYLLNKEALDGKYDAIL
                     TAHNLDDEAAFLFSNLTHWNMQYLGRQYPLLPATTGFVRKVKPFCMFYERETTAYALL
                     CGIQYIYTECPYAKGSKQLRNKIYLNMLEDEQAGFKSQFYAGYLRALDAGTFPRSQES
                     PEMLEQKRCTQCGQPTSSNGLCTFCRLFTR"
     gene            complement(1611..1805)
                     /locus_tag="PLL88_07755"
     CDS             complement(1611..1805)
                     /locus_tag="PLL88_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01496.1"
                     /translation="MLATLKIREKEIHIRGNRPLYRIMEDLQLSPQAYLAVRDGVLLT
                     GDDRIQEGDVVELIAVVSGG"
     gene            complement(1812..2828)
                     /locus_tag="PLL88_07760"
     CDS             complement(1812..2828)
                     /locus_tag="PLL88_07760"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOJ01497.1"
                     /translation="MENIAIPQTPAELFEMLKVQHPYLLRLLFWQNGRLVLDSHNPQA
                     KLDRETNLLKIFANMVIRVMPFMRSPLRDFYEDLKNVRSVTKSIISLLAGIVFGYEIT
                     ARLDDPIRTFLPEITHDDPKATIKLKHLLSNTSGLPTIDDLPSMRKLLSTQNWCETIL
                     RLPLQDQPGKGYFYSSANFHLVACILERTLGNSLYQFAQAHLFLPLGIQKTHWACDPQ
                     GVPFGGSDLYLRAEDMLSIGRLCLQDGSWNGQQIVPHDWLKLSTQPIVSVDEKNQYGY
                     GWWIDHNFQESRLHSFSACGVGGQRIMIIPAKNAVVVTTSLTSLNAHSNVIDDRVITF
                     LQGR"
     gene            3009..3314
                     /locus_tag="PLL88_07765"
     CDS             3009..3314
                     /locus_tag="PLL88_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01498.1"
                     /translation="MRDQFFEPVGEQGYYSAYDYVQKNVRNSVVWVENGLLFYAYDAS
                     FSNTVSRSSLPDYVVGFKTDWLGNGGGSYPTLIEEYMADPAVQIAYQDPQDIVLYCP"
     gene            3413..4612
                     /locus_tag="PLL88_07770"
     CDS             3413..4612
                     /locus_tag="PLL88_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ01499.1"
                     /translation="MHKSKFLNYLMFGLLYFTQGTVLAYFLSVNALYFNENGLSGLDL
                     GIFSTIAMIPFVIKILYGMFSDRYALLGMGHRKPYILIGLAVQVLCLVLVPFIDLKSG
                     YWLFVAVAFLLQMGMALYDTCTDGLALDTVPEEEEGTIQAIMVGGRALGLVLTAPLVG
                     LLAEYVGWNWVFWSMAISTLFPLPLVLGIHAEKKESAEKFNWSAFAAFKNRYVVAIAL
                     VGFVFFAVYAGVNAIINLSLEMRFAAEFNKAVAGLVSAVLGSGIILGGIFGGRVLDKL
                     GKKRAIWAALITSLVACLVFAFIPNPRWAWGVVFIVGLSFGVQQTIIFALSMNATDAR
                     IAASMYSILMAVTNVAQAVGMFLSASLTDSIGFVWVFVVMGAVNLLSIPFIHTIHRGK
                     ARTQPEA"
     gene            4621..4935
                     /locus_tag="PLL88_07775"
     CDS             4621..4935
                     /locus_tag="PLL88_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01500.1"
                     /translation="MELLEHNQKAWDKLARGGIEWSIPVSSEVIAEARNGNWQVFLTE
                     SKPVPRDWFPPDLHGVEVLCLASGVVSRRPSLPRLARRLLRLISLLNNSTATAWLQSG
                     KD"
     gene            4842..5393
                     /locus_tag="PLL88_07780"
     CDS             4842..5393
                     /locus_tag="PLL88_07780"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ01501.1"
                     /translation="MFAAAGAQVTSFDLSSEQLNRDRLVAEREGLALTTIQGDMRDLS
                     VFSDEHFDLIFHPVSNVFIPDVLPVWRECFRVLKQGGALLAGMSNPFEYCFDQELSEK
                     QGIYQVKYSLPYSDLTSISQEERCRIFGEDEPLEFSHTLEEQIGGQLAAGFVISGFYE
                     SCRADDPIAKYMPSYFATRASKP"
     gene            5636..8167
                     /locus_tag="PLL88_07785"
     CDS             5636..8167
                     /locus_tag="PLL88_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOJ01502.1"
                     /translation="MTPFETCTRTAQEVLQIFNSDQEKGLSADQVEKALQAQGSNEIN
                     AQEVHWWQILLRQFKSSFVYLLVGASLISWFFGEHLDAILIALFIVINSMIGFVQEYR
                     SEMTVKSLKKFTVGKARVRRDGVDHTIAATELVKGDIVILETGDIIPADLRVLKTHNF
                     LVDESVLTGESVQVNKTSEKMEKEVTQPFQAANLVFKGTSVMGGTAEGVVVATGKESE
                     IGKISHIITETKKESAFQKNINRISTFVMWMVGITILLLLTTHFLFKSSDAKSFTDLI
                     IFSIALTVGVIPEALPLVTTFSLSSGARKLAQDNVVVKRLTSVEDLGSIQVLCTDKTG
                     TITENKLSVVDVLRIDKEADPVFIGSLAVTNIYEQQNLPNNAFDLSLIARLSEAEKTE
                     LRAYHLVNEIPFDPTRRRNSVLLERADRRLLVVRGSPEEMLALDSQLSELRKKEILQW
                     LAGQGKMGRRTLAVSMADDLGSEEYDEKMESGHIRICGIVSFVDELKPTTRAVIEHAH
                     NLGVRLVILTGDDPVVAGAVGAEAGIIRHPEEVMTGAAFDNLSDQEKKEVVEKTNVFA
                     RVSPTQKYEILQVLKEKHQVGFLGEGINDAPALKSAHVSLVVESASDIARETADIILL
                     KQDLGVIIKGIFEGRKAFHNTVNYIRATLLSNFGNFYAVAFASLIIPYLPMLPVQLLT
                     LNLLSDFPMFSISTDNVAEKDLQNPKNYNTREIILIASLLGVVSTLFDFIIFGYFQRF
                     GESVLQTYWFMGSVATELVLLFSIRTDRVFFKAGAAPSRTIVAMTFVAFLTAMILPFT
                     TFGDTVFHFVKPAWNYLGVLALIVVGYFLCTEFAKNLYYRFLAKE"
     gene            8695..9738
                     /locus_tag="PLL88_07790"
     CDS             8695..9738
                     /locus_tag="PLL88_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01503.1"
                     /translation="MKVVLISCPKTERMQYKLRQFQKMTVAFEHGGVERQFHLIKSKK
                     DLVKILQVEQPDLVFSTDYYTRDDANMPVHIHAILEEMKIPYVGSDPQVLELVLSKAG
                     LKKKWALHDIPTPPFFTIKKLGSIIEGLDNLELAAGYPYILKPNRGGNSCGLDESSIV
                     FDKTALLSRMRELFKTYDEIMVEKYLGICPNIREFTVAMLGNGKHKLVMPARITLKQK
                     KGLRIITTRDKDEHMTQAVPVTDDALRKRLEVFSSRAFEVAGVRDYARCDILFANDLL
                     YAIEINGQPMIPDEWFAQCAMDVPLDTNQYLNAILLAAIARNNKRLRKKLPIPQPMSE
                     LLPARVFEQLTTA"
     gene            complement(9776..10705)
                     /gene="pfkB"
                     /locus_tag="PLL88_07795"
     CDS             complement(9776..10705)
                     /gene="pfkB"
                     /locus_tag="PLL88_07795"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HOJ01504.1"
                     /translation="MIYTLTLNPAVDRELIIPRLELDTVLRAKSVVVDCGGKGINVSR
                     ALLALGVRSVASGFLGGNNGKFLLEGLHTSGISTDFVWLEQETRINISIFAEHDGHYI
                     KVNENGPSVNSNDQQKMLAKVSELAQSGDWWILSGNLPPNVSQDFYATILKILNQQGA
                     NAILDTSGEALQFGLQERPFLVKPNAEEAGVLTGTSTETSVQLKNIATMIRGMGAQNV
                     IISLGKQGALLQTRGGTWLAHAPTIEQKNPTGAGDSMVGGMVYALQRGLGMKEALTWG
                     VACGAATASLPGTQVGSKNLVTSFLTDIHVEGI"
     gene            complement(10761..12038)
                     /locus_tag="PLL88_07800"
     CDS             complement(10761..12038)
                     /locus_tag="PLL88_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOJ01505.1"
                     /translation="MSHPIYDPALLSLRKISNKGANAESAYNQNRRLILQLIKDAGVI
                     SRKALAEKTGLKLATITMIIKELLEKGIIQETGFIDGDCGRKIMGFGLVRDKYCTIVS
                     RISVSYFTIGVYDVSNENLYIDKVFMDTLHDINKTCDAIAKEIRELKKKFQHKTILGV
                     AIGVEGPFVIKDGYYKMPDPSQPDGYFDIGKVLSKKISLPVIVNRQNNFAVYNLWMEE
                     NKNNHLGIFINITISYAIECGIMVNGEILNGCNGSAGLLGKVVFDPHTGNTFDELCSS
                     NSVLRRVHDSLPAYPQSVLNQINGEVNIRDVIKAFIQEDELAVAIFTDVGKSLGQTVA
                     SLTNLLNPDRILIGDEIPQTERMRGIIESEAKKYISPEVKLNLDMFILDFKSHRQAKI
                     DPSLLGASRYIVDAFIQSIDFTEISIDASQKQG"
BASE COUNT         2895 a         2810 c         3141 g         3359 t
ORIGIN      
        1 tctttggaat caacggttct ggcatgcact gccaccagtc cattttcgat aagagtggaa
       61 ataatctttt cttcgatgcc aaggatgaat ttcatctttc gaaattagcc tatggtttca
      121 tcgctgggca gctcagccat gccaaggcac tctctgctgt tgtggcgcct acggtgaatt
      181 cttataaacg cctggttccc ggctatgaag ctcccgtcta tataggatgg gcgcaaacca
      241 accgttctgc gttgatccgc atcccgcgct ataccatcgg aaaagacaaa tctatgcggg
      301 ctgagttgcg cttcccggat ccgtccagca atccctacct ggcgttcagc gccatgattg
      361 cggctgcggt ggatggcatt gagaagggtc tcactcctcc gaaaccattg aacaatgtca
      421 atatctacga tctttcacgc gaagaacgga tcacgcgggg gatcgaatcg ttgccgggtt
      481 cgctaaagga ggcactggtg gaattggctg ccgatcctat cgtaaaagat tctatcggcg
      541 caaagctcta tgccgctttc taccgcgcca aagaagagga atgggaaaac tatcgcattc
      601 atgtgaccga ttgggaaatt gagaattatt tgcaaagcgc atgatccaaa aatttgtcga
      661 aaagaccttc tgatcggaag gtctttttta tcttgtgaac aagcggcaaa aagtgcataa
      721 tccattgctg gatgttggct gaccgcactg ggtgcagcgt ttttgctcca gcatctccgg
      781 tgattcctgc gaccgtggga aagttcccgc atccagcgcc cgcaggtatc cggcatagaa
      841 ttggcttttg aaccctgctt gttcatcttc caacatgttg agatagattt tgttgcgcag
      901 ttgtttgctc cctttggcat aagggcattc agtataaatg tactggattc cgcataacag
      961 agcgtaagcg gtcgtttcac gttcgtaaaa catgcaaaag ggtttgacct tgcgcacaaa
     1021 tccggttgtg gcgggcaaca gcgggtattg gcgaccgagg tactgcatgt tccagtgggt
     1081 gaggttggaa aataaaaacg cggcttcgtc atccaggttg tgggcggtca ggatggcatc
     1141 atatttcccg tccagcgctt ctttgttcag aaggtaccgt ttgacgagac cgcattgcga
     1201 acagggtttt tgatgaccgc gtttggtgcg gctgccaagt tccgggatgc tttcgccgta
     1261 acgggctttg acgttgatcg tggtcaatcg caatccccgc tcgtttgcga aggtctgtgc
     1321 tgtttctccg gaaattgcag agtaattcgt ttcgccattg atccccaaat caaggtaaat
     1381 accatcgact tgatatccca gtttccatag gatatcccac agagctaatg aatcctttcc
     1441 accggaaacc gccagcaata aacgttcctc gtggcgaaac atgctgtatt ttttaatgat
     1501 gtgggctgtc tgggtgcgca tccaatcggg aaagtgatct tggcatagtg ccaggttgtg
     1561 atgttccata tgcatgaccg ctttttgacc gcacttcgtg catttcacga ttaaccccct
     1621 gaaaccacag cgatcaattc aaccacatca ccttcctgga tgcgatcgtc gccagttaaa
     1681 agcacgccat cccgcaccgc caggtatgcc tgcggtgaga gctgcaggtc ttccatgatg
     1741 cggtagagag ggcggtttcc ccggatatgg atttcttttt ctctgatctt tagcgtggcg
     1801 agcatgcttt tctatcttcc ttgtaaaaaa gttatgactc tatcatcgat gacattgctg
     1861 tgagcgttta aactggtgag ggaagtggtg acaacaacag cgttctttgc ggggatgatc
     1921 ataatgcgct gcccacccac tccgcatgct gaaaaggaat gcaatcggct ttcttgaaaa
     1981 ttatggtcga tccaccagcc gtagccatac tggttctttt catccaccga tacgattggt
     2041 tgcgtggaga gcttcaacca gtcatgtggg acgatctgtt gcccattcca gctaccatcc
     2101 tgcaggcaaa gtcgcccgat gctcagcatg tcctccgctc tgaggtacag gtctgatccg
     2161 ccaaatggca ctccttgcgg atcgcatgcc caatgtgttt tttgtatccc taaaggaagg
     2221 aacagatgtg cttgcgcgaa ttgatacaaa ctgtttccca atgtccgctc taagatgcat
     2281 gcgacgagat ggaaatttgc tgaggaatag aaatagcctt tcccaggttg atcttggagg
     2341 ggcaggcgca agatggtctc acaccaattc tgcgtggaga gcagtttgcg catggatgga
     2401 agatcatcta tggttggcag accggaagta ttgctcagga ggtgttttaa tttgatggtc
     2461 gctttggggt catcgtgcgt tatttctggc aagaaagttc ggatgggatc atccaatcgg
     2521 gcggtgatct cgtatccaaa aactatgcca gccagcaatg agatgatgga ttttgtgacc
     2581 gaacggacat tttttaagtc ctcataaaag tctctcaatg gagaacgcat gaatggcatg
     2641 acccgaatga ccatatttgc gaaaattttt agcaaattgg tctcccgatc taattttgcc
     2701 tgggggttat ggctgtccaa caccagccgc ccgttctgcc agaacagtaa ccgcagcaga
     2761 taagggtgtt gcaccttcag catttcgaaa agttcagcgg gagtttgggg tatcgctatg
     2821 ttctccatgt ggatttttcc ttgacttaac tgtatcgcat ttctacagaa ataatagagt
     2881 tcttgtatca gtagacctgc tctatttcat tgaagtttca ctattcatga tcgctgaaaa
     2941 gaggcagcat tttcttcgtt tattggcagc gaactggcat tgaagccgtt ccgcaaaatg
     3001 cgatcatctt gcgcgatcaa ttcttcgaac cggtggggga gcagggttac tacagcgctt
     3061 atgattatgt tcaaaagaat gtgcgcaatt cagtggtgtg ggtggaaaac gggttgctgt
     3121 tttatgccta tgacgcctcg ttcagtaaca ccgtcagccg cagcagccta cccgattatg
     3181 tggtcggctt taaaaccgat tggcttggaa acggtggtgg gagttatcca actttgatcg
     3241 aggaatacat ggcagatcct gccgttcaga ttgcctatca ggacccgcag gatattgtgt
     3301 tgtactgccc atgagggttc ctcacggatt ttctttatat agtggatatt cttcgtgaaa
     3361 tcaatacaat aatagaaatg ttttcataaa acaatcacga caggaatgat tcatgcataa
     3421 atcaaaattt ctcaattacc tcatgttcgg tttgctctat ttcacacagg ggaccgttct
     3481 cgcctatttt ctgagcgtta acgcattgta ctttaatgaa aatggcttaa gtggattgga
     3541 tctgggcatc ttcagcacta ttgccatgat accctttgtg atcaagattc tgtatggaat
     3601 gttcagcgac cgctatgcct tattgggcat ggggcatcgc aaaccgtata tcctgatcgg
     3661 tctggcagta caggtgcttt gcctggtgct ggtgccgttt attgatctga aaagcggtta
     3721 ttggttgttt gtggcggtgg catttttgtt gcaaatgggg atggcgcttt acgatacctg
     3781 caccgatggg cttgccctgg atactgttcc ggaagaagag gaaggcacca tccaggcgat
     3841 catggtaggt ggtcgggcgc tggggctggt tctaaccgct ccgctggtgg ggttgctggc
     3901 ggaatacgtt ggttggaatt gggttttctg gagcatggcg atctccacgc tgtttccgct
     3961 gccgctggtg ctgggtattc atgccgagaa aaaagaatca gcagaaaaat tcaattggag
     4021 tgcgtttgct gccttcaaaa atcgctatgt ggtggcgatc gcgctggttg gtttcgtctt
     4081 ctttgcggtg tatgccgggg tgaatgccat catcaatctt tccctggaaa tgcgttttgc
     4141 tgccgaattc aacaaagctg tggcagggtt ggtctctgct gtgctgggca gcggtatcat
     4201 cctgggtggc atctttggcg ggcgagtgct ggataagctc ggcaagaaaa gagccatttg
     4261 ggcagctctg atcacgtcat tggtcgcttg tctggtattc gctttcattc ccaatccccg
     4321 ctgggcatgg ggcgtggtat tcatcgtagg gctctctttt ggtgtgcaac agactatcat
     4381 ctttgccctc tccatgaatg ccacggatgc gcgcattgcc gcctccatgt attcgattct
     4441 catggcggtc accaatgtag cacaggcggt gggcatgttt ttgagcgcca gcctcaccga
     4501 tagcattggt tttgtgtggg ttttcgtggt gatgggtgcc gtcaacttgc tctccatccc
     4561 gttcattcac accattcatc ggggaaaagc caggacccaa ccggaagcgt aggtgaggag
     4621 atggaattgc ttgaacataa tcaaaaagcg tgggataagc tggcaagagg tggtatagag
     4681 tggtcaatcc cggtttcttc cgaggtgatc gccgaagcgc gcaacgggaa ctggcaggtt
     4741 tttctgacgg aatcgaaacc ggttccgcgc gattggttcc cgcctgacct gcatggtgtt
     4801 gaggtgttgt gcctggctag cggggtggtc agcaggcgcc catctttgcc gcggctggcg
     4861 cgcaggttac ttcgtttgat ctctcttctg aacaactcaa ccgcgaccgc ctggttgcag
     4921 agcgggaagg attagccctg acaacaatcc agggtgatat gcgcgacctc tctgtttttt
     4981 ctgacgaaca tttcgatctg attttccacc cggtctcgaa tgtcttcatt ccggacgtgc
     5041 tgccggtttg gcgtgaatgt ttccgcgtct tgaagcaggg cggcgctttg ctggcgggca
     5101 tgtcgaaccc ctttgaatat tgctttgatc aggaattgag cgaaaagcag ggcatttacc
     5161 aggtaaagta ttctcttccg tattccgatc tgaccagcat ctctcaggaa gaacgttgcc
     5221 gcatcttcgg agaggatgaa ccactggaat tcagccatac gttggaagag cagatcggcg
     5281 ggcagcttgc agccggtttt gtcatcagcg ggttttatga atcctgccgg gctgatgatc
     5341 cgatcgcgaa gtacatgcca tcctactttg caaccagagc gtcaaagcca tagaaggttt
     5401 aggttatcat caaagacatc cctctattcg tggaaggttt tgatgaacgt tcgtagccat
     5461 ggggtcgact ttcattcttc ctcgaacgaa aaaattcctt cgaacctgac gaaaccgctt
     5521 gaccgtagaa cattccattt gctgatgccg gaatcagcat ggtattcacc ctcatgattt
     5581 cgctgataat atagatacgt cgatgtacaa tgaattcaat gtggttggag gagagatgac
     5641 accatttgaa acctgtacca gaactgcaca agaagttctg caaattttta atagcgacca
     5701 agagaaaggg ctatccgctg atcaggttga gaaagcgctg caggcacagg gcagcaatga
     5761 gatcaatgct caagaggtgc actggtggca gattcttctg cgacagttca aatcttcctt
     5821 tgtgtacctg ctggttggcg cctccctcat ttcctggttc tttggcgaac acctcgatgc
     5881 gatcttgatt gctctcttta ttgtgatcaa ttcgatgatc ggttttgttc aagagtaccg
     5941 ctcggaaatg accgtcaagt cgttgaagaa attcacggtg gggaaagctc gcgtgcgccg
     6001 cgatggcgtt gatcacacca ttgccgcgac ggagctggta aagggcgata ttgtcattct
     6061 ggagaccggt gatatcatcc cggcggatct gcgggtgctc aagacgcata attttctggt
     6121 ggatgagtcg gtgctgaccg gcgaatcggt ccaggtcaat aaaacttccg aaaaaatgga
     6181 aaaggaagtg acgcaacctt ttcaagctgc caatttggtg ttcaaaggca cttctgtgat
     6241 gggcggtacc gcggaaggtg tggtggtagc cactggaaaa gaaagtgaga tcggaaagat
     6301 cagtcatatc atcacggaga ccaaaaaaga gagcgcgttt cagaagaata tcaaccgtat
     6361 cagcactttc gtgatgtgga tggtggggat taccatactt cttttgttga ccacgcattt
     6421 tcttttcaag agcagcgatg cgaagtcctt cacagatctg atcatcttct ccatcgcgct
     6481 cacggtgggg gtcattcctg aagcgttgcc gttggttact accttctcgt tatccagcgg
     6541 tgccaggaag ttggcgcagg ataacgtggt ggtgaaacgg cttacatcgg tagaagacct
     6601 ggggagcatt caggtgttat gcacggataa gactggtacg atcacagaaa ataagttgag
     6661 tgtcgttgac gttttgcgca tagataaaga ggcggatccg gtttttatcg gcagtctggc
     6721 ggtcaccaat atctatgaac agcagaactt gcccaataat gcgttcgacc tttccttgat
     6781 tgcgcgcttg agcgaagcag aaaaaactga gctgcgtgca taccatcttg tcaacgaaat
     6841 tccctttgat ccaacccgcc ggcgcaacag cgtgttgctg gaaagggcgg atcggcgttt
     6901 gttggtcgtg cgcggatcgc ctgaggagat gcttgccctt gattcccaat taagtgagct
     6961 tcggaaaaaa gaaattttgc agtggctcgc cgggcaggga aagatgggaa gacgaaccct
     7021 ggcagtttcc atggcagatg atcttgggag tgaagaatat gatgaaaaaa tggagtccgg
     7081 gcatatccgc atctgtggta tcgtctcatt tgtagacgaa ttgaaaccca ccacgcgtgc
     7141 ggtgattgaa catgcgcaca atttgggcgt gcgcctggtg atcctgaccg gtgacgatcc
     7201 ggttgtggcg ggtgcggtgg gtgctgaagc ggggatcatc cggcatcccg aagaggtgat
     7261 gaccggggct gcttttgata acctgagtga ccaggagaag aaagaggtgg tggaaaagac
     7321 gaacgttttc gcgcgcgtct cccccaccca aaagtatgag atattgcagg ttctaaaaga
     7381 gaagcatcag gttggatttc tgggcgaggg gatcaacgat gcacctgctt tgaagagcgc
     7441 ccatgtatcc ctggttgtag aaagcgcgtc ggatatcgcc cgtgaaacgg cggatatcat
     7501 cctgctcaag caggatctgg gggtcatcat caaggggatc ttcgagggtc gtaaagcatt
     7561 ccataacacc gtgaattaca ttcgagccac gctcctttcg aatttcggaa acttctatgc
     7621 ggtggcattt gcttcgctga tcatccccta tttgccgatg ctgcctgtgc aattgctgac
     7681 cttgaacttg ctttctgatt ttccgatgtt ctctatttca acagataatg tcgcagaaaa
     7741 ggatctgcag aatccgaaaa attacaacac gcgtgagatc atcctgatcg cctcgttgct
     7801 tggggtggtg agcactttgt tcgatttcat tatttttgga tattttcagc ggtttggaga
     7861 gagtgtgctg caaacctact ggtttatggg tagcgtagcc accgaattgg tgctgctgtt
     7921 ctccatccgt accgaccggg ttttctttaa agcgggggcg gctccttctc gaactattgt
     7981 agccatgaca tttgtggcgt ttctcacggc gatgattctg ccctttacga cttttggcga
     8041 tactgttttt catttcgtaa aaccagcctg gaattatttg ggtgtgctgg ctttgatcgt
     8101 ggttggatat tttctttgta cggaatttgc gaagaatctc tactatcgtt ttctggcgaa
     8161 agaataatag ggatggtgtg ccgaatgcgc gctagataga atagagcgac cagtaaaatt
     8221 tttgggcggt gcaccgtttg gattgcaaga ttaagcttgc aggttggaat cgaaagaacg
     8281 aaataagagg gaaggttttg tggttgattg cacttgttaa agacagtaga ttgacaactt
     8341 taacctctgt aaccagcctt tgtcctactt ggcgcatgga ggatggcgta cttgctgggc
     8401 agatatccct ttgctggaag gagactcgac cactctgctg gtatttggca ggggcatatt
     8461 ctcaatagga tagtgtaaat gtggtaagat ttttgtgcgc gcccatgacc ggctgacttc
     8521 tcgccaaaac aaaatacatg cactgatagc attcctgtta atttcttgtt ggttcatcaa
     8581 gtaggatttc gcctgaataa tcccgttggt cgaaaaacac tagcatggta acgaattgaa
     8641 gccagcctca gttcgggttc aatgctggct aggttcaaat ttaaggagga agtcttgaaa
     8701 gtagttctca taagctgtcc aaaaacagaa cgaatgcagt ataaattgcg acagttccaa
     8761 aaaatgaccg tagcgttcga gcatggcggg gtcgagagac agtttcatct tatcaaaagc
     8821 aaaaaagatt tggtgaaaat tttgcaagtg gaacagcctg atctggtctt cagtaccgat
     8881 tactataccc gggatgatgc caatatgcct gttcatatcc acgccatcct ggaagagatg
     8941 aaaattccct atgtcggttc agatccgcag gttctggaac tagtgctttc caaagcgggt
     9001 ctcaagaaga aatgggctct tcatgatatt cccaccccac cgtttttcac tatcaagaag
     9061 ctcggctcta taatcgaagg gctcgacaat ctcgaactgg ctgctggcta cccctatatc
     9121 ctgaaaccga acaggggtgg taacagttgc gggttggatg agagttccat tgtgtttgat
     9181 aaaactgctc tgctttccag gatgcgggaa ctcttcaaaa catatgacga gatcatggtc
     9241 gagaaatacc ttggcatttg ccccaatatc agagaattca ctgtggcaat gctgggaaat
     9301 gggaaacaca agctggtaat gcctgccagg atcaccctca aacaaaagaa agggttacgg
     9361 atcatcacca ccagggataa agatgagcac atgacccagg ctgtgccggt gaccgatgat
     9421 gcattaagaa agcgactgga ggtattttct tccagagctt tcgaggtcgc tggagtgcgc
     9481 gattacgctc gttgtgacat tctgtttgcg aacgatctat tgtatgctat agagatcaat
     9541 gggcagccga tgatccctga tgaatggttc gcgcagtgtg ccatggatgt tccccttgat
     9601 acgaatcagt atctgaatgc catcctgctc gctgccattg cgaggaacaa caagcggctg
     9661 agaaagaagc tgcccatccc tcaaccgatg agcgaactgc tgcccgcgcg tgttttcgaa
     9721 caactgacta ctgcctgact gaaattggga gcactgtttg caaggcagaa ggtagctaaa
     9781 ttccttccac atggatatct gtgagaaaac tggttaccag gtttttgctt cccacttggg
     9841 tgcctggcag actggcagta gctgcgccgc aagccacacc ccaggtgagc gcttctttca
     9901 tacccagacc tcgctgcagg gcgtaaacca tccctccgac catcgaatcc ccggcgccgg
     9961 tgggattctt ctgttcaatc gtaggagcgt gagccagcca ggtgccgcca cgggtttgca
    10021 aaagcgctcc ttgtttgccc aacgaaatga tcacgttctg cgctcccatg ccgcggatca
    10081 tggtggctat gttttttagc tgcacgcttg tttcagtaga cgtacctgtt agaacacctg
    10141 cttcttccgc attgggcttg acgagaaaag gtctttcttg cagaccaaac tgcaacgctt
    10201 cgccgctcgt atccagaatg gcatttgctc cttgctggtt gagaattttc agaatggtcg
    10261 cgtaaaagtc ttgcgatacg ttgggaggga ggttgcctga cagaatccac caatctccac
    10321 tctgtgccag ctcgctcact ttcgcaagca ttttctgctg atcattgctg tttacggatg
    10381 gtccgttttc attgaccttt atataatgcc cgtcgtgctc tgcaaaaatg ctgatattga
    10441 tgcgtgtttc ctgttctagc cagacgaaat cagttgatat tcctgaagtg tgcagccctt
    10501 ccagtaaaaa cttgccattg tttcctccta aaaaaccgct cgccacgctg cgaacgccta
    10561 gggcaagcag agcacgggat acgttgatac cttttcctcc acagtcaacc accacgctct
    10621 ttgcgcgcag aacggtatcc aactccaggc gggggatgat aagttcgcgg tcgacagctg
    10681 ggttcagagt tagcgtatag atcattcctg ttttcccgaa ttcatgaatt cgatcttgcc
    10741 tgcgtttttg atggattgac tcatccttgc ttttgagaag cgtcaatgct gatctctgtg
    10801 aaatcgatgg attgaatgaa tgcgtccacg atatagcggc tggctcccag caaactgggg
    10861 tcgatctttg cctgccggtg gctcttgaaa tcgagaatga acatatccag attaagcttt
    10921 acttcgggcg aaatgtactt ttttgcttcg ctttcaatga tgccgcgcat tctttccgtt
    10981 tgtggaattt catctcctat gagaatgcgg tcggggttca ataaattggt gagggaagcg
    11041 actgtctgcc ccagcgattt tccaacatct gtgaatattg ctaccgccag ctcatcttcc
    11101 tgaataaaag ctttgatcac atcacggatg ttgacctcgc cattgatctg gttgagcaca
    11161 gattggggat atgctggaag ggaatcatgg acgcgccgca ggacagaatt agaagagcaa
    11221 agctcatcga aagtgttgcc ggtatgtggg tcgaatacca cttttcccaa taatcctgcc
    11281 gatccgttgc acccgttcaa gatttccccg tttaccatga tcccacactc gatggcatag
    11341 ctgatggtga tattgatgaa tattcccaaa tgattgtttt tattttcttc catccacagg
    11401 ttgtaaacag caaagttgtt ttgtctgttt acaatgaccg gcagggagat ttttttggaa
    11461 agcactttcc cgatatcaaa atatccgtct ggctggctgg ggtcaggcat tttgtaataa
    11521 ccatctttga tgacaaatgg accttccacc ccaatcgcaa ctcccaggat ggtcttgtgc
    11581 tggaacttct ttttcagctc cctgatctct ttcgcaatcg catcacatgt tttattgata
    11641 tcatgcagtg tatccatgaa gaccttatcg atatacaggt tttcgttgct gacatcataa
    11701 acgccgatcg tgaaatagga aacgctgata cggctgacaa tcgtgcagta tttatcgcgc
    11761 accaagccaa aacccatgat cttgcgtccg caatctccat caataaaacc ggtctcctga
    11821 ataatccctt tctctagcag ttccttgatg atcatggtga tagtagccag tttcaaccca
    11881 gttttctctg ccagtgcctt tcgcgagatc acccctgcat ctttgatcag ttgtaggatc
    11941 aatctgcggt tttgattata ggcgctctca gcgttagcac ctttattgct aattttcctc
    12001 agcgaaagca aggctggatc gtaaatcgga tgactcacat tccctccata aaatgattgc
    12061 tggataattt ctttatctta tcagaaaaac acatgtcttg tccttttttt gccaatgttt
    12121 ctccaggttc ttttccagac atctaagggc aggggatgag agaatcctcg tcttgctcaa
    12181 aagaacggga ttttaagaaa aagat
//
LOCUS       DAOSDS010000061        12190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2018_length_12190_cov_3.981047, whole genome shotgun sequence.
ACCESSION   DAOSDS010000061 DAOSDS010000000
VERSION     DAOSDS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12190
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2018_length_12190_cov_3.981047"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="PLL88_07805"
     CDS             <1..1117
                     /locus_tag="PLL88_07805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ01506.1"
                     /translation="KARIADSMRVTGIEALYERSLYELSGGEKQKVAIASVLALQPSI
                     LMLDEPTSNLDLKSARMIFEVLAKLQQTHQLTVIIIEHKVSLFQDLFKRCVHLENGKI
                     TFDGPITQSPFSNASLKLFPKGNKGETVGRAPILELKDWSCQYGNDFRLAIDALEIYS
                     GEIIALVGANGSGKTTLLKSLAGLVGAQNGQLTFRGAVLPMVPLPQIGLVFQNADEQI
                     FMNTVLDEVTYGLKNFHLHDDLHARQMQMLFDQFGLSGKASTNPHQLSYGEKKRLNLI
                     STLVLAPCILLLDEIFIGQDMYLVNFILERLCLLRNQGVTVIAAIHDPAMMHRMADRI
                     LFLEDGKMRFCLPLQQALIWWQVNGYRDYLLEKELRI"
     gene            1114..1890
                     /locus_tag="PLL88_07810"
     CDS             1114..1890
                     /locus_tag="PLL88_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOJ01507.1"
                     /translation="MKKIAFAPDHTWIHALHPFTKFTILIGLSVLVFFIRSAQGMVLV
                     FAFILSCFFIASRNPFVYFGMRTTVVTALMIGIIQCIFTRQGTEVFNLLRFTITDYGI
                     QRAIVISVRFLVIVLGSYLFILTTSPSDFAFAIMQMGVPYRYAFLIVTSMRLVPILSI
                     DGERIMDAQRLRGARYDVRTPRLALLHMNAFLGAVLYSLVERVNTLSISMEGRSFGRY
                     HTRTYQQSIRFAVRDGVAFGCTLLLISIIFYIDKKWKILL"
     gene            1887..2834
                     /locus_tag="PLL88_07815"
     CDS             1887..2834
                     /locus_tag="PLL88_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01508.1"
                     /translation="MKTAKKTKPMTKTIKIISILIGVVALGALVVSLSMKQLPVRVLT
                     DYPFGTLWEEGVTMHECAECHDPETDFHSCSTCHDEHGSVELPNLSFYNMIELTGDVA
                     EVTFVPWNHFFNSFADLPNTFITMDEFVTKWEVPDYESITLYTRDGEFVTIKKSEITD
                     NAKFLPYEDGIRFASDDLHVSTWAKGIAKIIIVGTERSLEVEGQKTSLGRLLLGKTTS
                     ITIEEAKVMFRSEEDGLIREAITSSRVEGAAITDLLDLANYENVVFTQQDGTTITLPV
                     EEIREGVLTKQKASVVLIIPGKGRNNWIFDIVRIEGNVQ"
     gene            2831..3967
                     /locus_tag="PLL88_07820"
     CDS             2831..3967
                     /locus_tag="PLL88_07820"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOJ01509.1"
                     /translation="MKRWLRIFLILACVGLSACSNAGGDLVLFIDGKESMVTKVDYTE
                     IEEKVNVDDDEDFSLLLEYLFYKMGVKAVDEVTLITEESAQTFSWDEIADSTYWRNTR
                     QIEIHGEEYIVDQVAVTTEGFEEDVEHSITDIAPLTAEALGIPFGERESGFDIQAGSY
                     EHVVWLFLDGFGYEGFQAAKSEGLIPYLGSLGEIFPSYTIYPPRTSTSTAALLSGLDS
                     QQNGVWATGIRNLGVPSIVDALIEVGRGVTIVEGSSTPFNYPNAVVVLSGDRNGDGST
                     DDNVLMNAKETIANGVDELLMIHFHGIDDAGHTYGPYSPQWNAKIPEIDSSVEQLIEL
                     LPAKTLVIIFPDHGMHSVENDTSLGNHGNLIFEDMGTYIILYQK"
     gene            4001..4480
                     /locus_tag="PLL88_07825"
     CDS             4001..4480
                     /locus_tag="PLL88_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOJ01510.1"
                     /translation="MTKKNAVLLTTVLLMIFLVGCGAKHPAVDWTLKISGDVATPLEL
                     SFADLATMNQVELNDILMEKSTGEDEVTSWSGVALDELFAQAGAPTDYVSITAIASDG
                     YSIEVTKEELTGGIIALKDAGEWIVEVSPDKGPIRMVAPLTPANRWVYSIMEIQVNQ"
     gene            complement(4544..6775)
                     /locus_tag="PLL88_07830"
     CDS             complement(4544..6775)
                     /locus_tag="PLL88_07830"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOJ01511.1"
                     /translation="MEIDTLLAKLPEIYTVVDRDLILRAYKFAEKAHEGQKRLSGEPY
                     ITHCLAVAEILADLQVPPEMIAAALLHDTVEDTPVTLEDLENKFGAEIAKLVDGVTKL
                     TNLPRVARADQMVEKKEATSAQGNAEIDEDGEDSDLKHARSRRHELATETLRKMLLAM
                     GDDIRVVVIKLADRLHNMRTLSHMPEKKRYSIAKETLDIFAPLANRLGIWQLKWELED
                     LSLRYIKPEMYKEIAQKLSDRRKDREEEIALIIKDLDAVMKKGKIDAEISGRPKHIYS
                     IYRKMTDKGMPFEMVRDLRGVRLIVPDIPSCYAALGLIHTHWRPIPNEFDDYIAAPKD
                     NFYRSLHTAVIYSDGKTLEVQIRTSEMNQNAEFGIAAHWMYKEKKPFSDTYQQRINWL
                     RKLMDWKQDVDDAQEFVDGMKSDVFEDRVYVFTPRGDIIDLPRGSTPIDFAYHVHTEI
                     GNRCRGAKVNGKLVSLDYHLQTGDQLEILTSKHGGPSRDWLNINLGLVKTQRARSKIR
                     QWFKKQDEEQNLNQGRSILDKEFRRLGLENVNIENLAHSLDLKNEEELYISLGCGDLS
                     LRKLISKLDDVEKDNEDPLFKVHAPVAEQEGADTVNVLGLKGILTHFARCCSPAPGDA
                     IMGYITRGRGVTIHRQDCPNMLSVKDHERIIKVNWGEAHRTFPVDVQIKAYDRQGLMN
                     DISSILSNEGINLRDIKINLSNNLAVINLTLDITDITQLSRVLTRIESLPNIMEAFRF
                     RPG"
     gene            complement(6843..7505)
                     /locus_tag="PLL88_07835"
     CDS             complement(6843..7505)
                     /locus_tag="PLL88_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ01512.1"
                     /translation="MKIKGIIFDFDGLILDTETAEVHIWEKVFDHFQLTFPLERYLDY
                     IGSATDNRFVQDIMREMSLSNDQIRLALELYDHFTLTSGPFDNPREGVVNTIQTGKAL
                     NLRLAIASNSHRDWVSGHLQNLKLDHVFDPVCTRDQVSHSKPHPEIYDLVLEKWRLPP
                     SEVIAFEDSPSGIQAAKNAGIFCIAVPNPLTQKLPLDKADRIYASFNDINLRELIENF
                     ES"
     gene            complement(7510..8610)
                     /locus_tag="PLL88_07840"
     CDS             complement(7510..8610)
                     /locus_tag="PLL88_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOJ01513.1"
                     /translation="MEQDTIHVPVTKLYDFMLAVFLKAGVPQADARMCADVLIKSDLR
                     GIESHGIGRLRMYYERLKSGLIEKEARLTVVRESPTTAVIDGNHGLGMVVATKSMQMA
                     ITKAQEFGMGAVAVRNSTHFGIDGFYPLMAVQQDMIGMSFTNARPCVSPTHGTQPTLG
                     TNPISFGAPTDEDCPFLFDGATSITQRGKIEVLARAEEALPVGWVIDQDNKPMTDPDQ
                     ILQEIVHDRAALLPLGGVDELNGSHKGYCLSTIVEILSSALQSGAFLFQLSGLGENNQ
                     PTHFRVGHFFLAMNIENFLPVNEFKKNIGDLLRTLRNTRKAPGKPRIYTAGEKEFEME
                     KIVARQGVPVNVNLQKDILYLQQALGLTDAIF"
     gene            complement(8640..9797)
                     /locus_tag="PLL88_07845"
     CDS             complement(8640..9797)
                     /locus_tag="PLL88_07845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOJ01514.1"
                     /translation="MPNANYDVIIIGAGSIGVPAAFSLSKVGLKVLVLDNRSSIGQVS
                     NKAAIGGIRATHSDPSKAWLCQRSLQILSIWKETYGDEIEWKKGGYVYAVYRKEDEIS
                     PKNLLLQQKACNLNIDWLDSKAMLDVVPDLNPQGLLGGTFSPDDGHASPLLSLFAFYK
                     HAVARHAEFHFNESVIGFTKHNSKIVGVQTTRGHYSTAVVINAAGAWAGEMAKLAGVD
                     IPVSPESHEAGITEPVQIFLDPLIVDMRPIDVTSNFYFYQHSTGQVLFCYSPEPPVQG
                     FDTQSTSDFLPIAAKRLLALIPRLQNIRVRRTWRGLYPMTPDGSPFLGFVPDLPGLLL
                     AVGMCGQGFMLGAGVGELLAATICGSLDKTQQQVLQNLSLSRSISHIEMLK"
     gene            complement(9790..11904)
                     /locus_tag="PLL88_07850"
     CDS             complement(9790..11904)
                     /locus_tag="PLL88_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ01515.1"
                     /translation="MQKINHRIESHPILPINRGKKVNFTWNGQPCEGYENESIASALI
                     ANGYHVFQYHHKDHFPQGIFCANGQCSQCMLLVDGFPRKACMEPVREGIQLASLNGHY
                     ALPESSPAEQHPIIRKSVQVLIIGAGPAGLSAAIELGKHGVETLLVDDKSEAGGKLVL
                     QTHRFFGSKEFVYAGTRGIDIARQLAGEATHCTSVEMWLNSPAVAVFSDHKVGVLKDN
                     RQYALIDPEILVVATGAREKSLRFSGNSLPGILGAGAFQTLLNHDWVTPGQTYFVVGG
                     GNVGLIAAYHAIQAGIHIAGLAELLAQCGGYKVHLDKLKRLGVPIFPSHTILRANGAD
                     QVKSVTIAEVDESFQPIPNTEKTIPCDGILIAAGLSPVNELFQQAQEFGFQVHAAGDA
                     EEIAEASAAIVSGKITAQKILTQLQKGSVSDAELTELMRFKQVLSSKPGPIRDSKTIH
                     TSSKVHPVFHCVQEIPCNPCSGLCVQHLIQIPPDDILSIPDFFDSEGKCNGCAQCVIG
                     CPGLAITLVDYRKDARFPIVTLPCEFHINKDDVIGQSFPVTDREGSVIGVAEVVQAVQ
                     SSKMDHTSLLKLVAPGEIAERIAGIHLEKIPPLPTPNWVKGQEEQETIICRCERVSEK
                     ELRDLIRGGMHDLNQLKAITRAGMGACGGKTCEALILQIMQEEGVQKDEITSFSKRPL
                     VVEVPLITFVEKDAREGDSDHA"
     gene            12048..>12190
                     /locus_tag="PLL88_07855"
     CDS             12048..>12190
                     /locus_tag="PLL88_07855"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOJ01516.1"
                     /translation="MKKIAVLTSGGDAPGMNAAIRAVVRTGIQKGMEVFGVPLGYSGL
                     IDG"
BASE COUNT         3143 a         2781 c         3035 g         3231 t
ORIGIN      
        1 gaaagcacgt atcgcagatt ctatgagagt tacggggatc gaagcgttgt acgaaagatc
       61 tctgtatgag ctctctggtg gggaaaagca aaaagtagcc atcgcatctg tgctggcgct
      121 acaaccatct atcctgatgc tggacgaacc gacatccaat cttgatctta aatcagcgcg
      181 catgattttt gaggtgctgg caaaattgca acagactcac cagttgacag tgatcatcat
      241 tgagcataag gtttctttgt ttcaagacct gttcaaacgg tgtgtgcatc tcgagaatgg
      301 aaaaatcact tttgatggac cgatcaccca atcccctttt agcaatgcgt ctctgaagct
      361 tttccctaaa ggaaataagg gagaaactgt cgggagagcg ccaatcctgg aattgaaaga
      421 ttggagctgc cagtacggaa acgactttcg gttagcaatc gatgcccttg aaatctacag
      481 tggggagatc atcgcgctgg taggcgccaa tggatcagga aagacaacct tgctaaaatc
      541 gttggcgggg ctcgttggcg cacaaaacgg gcaactcacc ttccggggag ccgtgctccc
      601 gatggtaccc cttccgcaaa tcgggctggt atttcaaaat gccgatgaac aaatattcat
      661 gaatacggtg ctggatgaag taacgtatgg tctaaagaat tttcatttgc atgatgatct
      721 tcatgcccga caaatgcaaa tgctgtttga tcaatttggt ttgagcggaa aggcttcaac
      781 aaatccacac caattaagct atggagaaaa gaaaagactg aacctgatct ctaccctggt
      841 gctggctcct tgcattttgt tgttggatga aatatttatc gggcaagaca tgtatctggt
      901 gaatttcatt ctagaaagat tgtgcttatt acgcaatcaa ggagttacgg taatcgcggc
      961 tatccatgat ccggcgatga tgcatagaat ggcagatcgc attctctttc tggaggatgg
     1021 caaaatgcgc ttctgcctgc ctttacaaca agcgctcatt tggtggcagg tgaatgggta
     1081 cagggattat ctgctggaga aggagctgag gatatgaaaa agattgcctt cgctccagac
     1141 catacgtgga tacatgcact gcacccattc acaaaattta caattttgat cggtttgagt
     1201 gtcctggtct tttttatccg atcagcgcag gggatggttc tggtttttgc tttcatctta
     1261 agttgctttt tcatcgcttc tcgcaaccct tttgtttatt ttgggatgcg cacaaccgtg
     1321 gtgacggcgc tgatgatcgg aatcattcaa tgcattttca cacgccaggg aacggaggtg
     1381 ttcaacctgt tgcgctttac gatcacagat tacggaattc agagagccat cgtgatatcc
     1441 gttcgttttc tggtgattgt tctcggcagt tatcttttta tccttactac ttctccgtca
     1501 gatttcgcat ttgcaatcat gcagatggga gtaccctatc gctatgcttt tctgatcgtg
     1561 acgtcgatgc ggctggtacc tattctatcc atcgatgggg agcgtatcat ggacgctcaa
     1621 aggttgagag gtgcacgcta tgacgtccga acgccacgcc tggctctcct gcatatgaac
     1681 gctttccttg gagcagtttt gtattccctg gtagaacggg tgaatacatt atctatttcc
     1741 atggagggga gaagttttgg cagatatcac accaggacat accaacaatc gattcgattt
     1801 gccgttcggg atggcgttgc atttggatgc acgcttctgt tgatctcgat aattttctat
     1861 atagataaga aatggaaaat acttctatga aaacagcaaa gaaaacaaaa cccatgacca
     1921 aaacgatcaa gatcataagc attctgatag gcgtagttgc tttgggtgca ctggttgttt
     1981 ccctttctat gaaacaatta cctgttcggg tgctcacgga ttacccgttc ggtactttat
     2041 gggaagaagg ggtaaccatg catgaatgtg cagaatgcca tgacccagag acggatttcc
     2101 attcatgcag cacctgccat gatgagcacg ggtcggtcga gctacccaat ctctctttct
     2161 ataatatgat tgagttgacc ggggatgttg ctgaagtgac ctttgttcca tggaaccatt
     2221 ttttcaacag tttcgctgat ttgcccaata catttattac gatggatgag tttgtaacca
     2281 aatgggaggt acccgattac gaaagcatca ccctctacac acgcgatggt gaatttgtaa
     2341 ccatcaaaaa gagtgagata acagataatg ccaagttttt gccttatgaa gatgggatcc
     2401 ggttcgcgtc ggatgatctg cacgtttcca cctgggcaaa agggatcgca aagatcatca
     2461 tcgttggaac agaaagatcg cttgaagtcg aaggacaaaa aacttccctt ggcagattac
     2521 tgcttgggaa aaccacctcc atcaccattg aagaagcaaa agtgatgttc cgcagtgaag
     2581 aagatggatt gatcagagaa gcaatcacgt caagccgggt agaaggtgca gctatcaccg
     2641 atctgctgga cctcgctaac tatgaaaatg tggtcttcac gcaacaggat ggcacaacca
     2701 ttacgttgcc ggtggaggag atccgagagg gggttctgac caaacaaaag gcgtctgttg
     2761 tgctgatcat tccgggtaag gggcgcaaca attggatttt tgacattgta aggatagaag
     2821 ggaatgtgca atgaaacgat ggttaaggat cttcctgata ctggcttgtg ttgggcttag
     2881 tgcctgttcc aacgctggcg gcgaccttgt tcttttcatt gatgggaagg aaagtatggt
     2941 aacaaaggtt gattacacgg agattgaaga aaaggtcaat gtggatgatg acgaagattt
     3001 tagcctgctg ctggaatacc tgttctacaa aatgggcgtg aaagctgtgg atgaggtcac
     3061 attaatcaca gaagaatcgg cgcagacatt ctcgtgggat gagattgccg attctaccta
     3121 ttggcgtaat acacgccaga tcgagatcca tggagaagaa tatatcgttg atcaggtcgc
     3181 tgtcacgacg gagggattcg aagaagatgt ggaacattcc ataacagata tagccccatt
     3241 gaccgcagaa gcgctgggca tcccatttgg cgaacgggaa tcgggctttg atatccaggc
     3301 aggatcatac gaacatgtag tttggttatt cctggacggt ttcgggtatg aaggttttca
     3361 agcagcgaag agtgaagggt tgattcctta tttgggctcc ttgggagaaa ttttcccttc
     3421 ctataccatc tatcccccga gaacaagcac atccaccgca gcgcttttgt cagggctgga
     3481 ttcgcaacaa aacggggtat gggcgaccgg cattcgcaac cttggggtgc ccagcatcgt
     3541 tgacgcactc attgaggtgg gcaggggcgt gacgatcgtt gaagggagca gtacgccctt
     3601 caattatccc aatgcagtgg ttgtcctatc tggtgataga aatggagatg gatcaacgga
     3661 cgataacgtt ttgatgaatg ccaaagaaac gatcgccaat ggggtggacg aactattgat
     3721 gatccatttt catgggattg atgatgcagg gcacacctat ggaccgtatt ctccacagtg
     3781 gaatgcaaaa atcccagaaa tagattcttc tgttgagcag ttgattgaac tgctgcctgc
     3841 aaagaccctg gtgatcatct tccctgacca cgggatgcat tctgtggaaa acgatacgtc
     3901 gttaggaaac catggaaatc tgattttcga ggatatgggc acgtacatta ttttgtatca
     3961 aaaataagga gttcgaaagc caaaattgaa aggagcaaaa atgacaaaga aaaatgctgt
     4021 acttctcacg actgtgttgc taatgatttt cctggtaggt tgtggcgcaa aacatcctgc
     4081 tgtggattgg actttgaaaa tatccggaga tgtagcaacg cccttggaat tatcttttgc
     4141 cgatctggca accatgaatc aggtagaatt gaatgatatc ctgatggaga aatctaccgg
     4201 tgaagatgag gtcacttcat ggtcaggggt tgcactggat gagctattcg cccaagcagg
     4261 agcaccaaca gattatgtct ccataacagc catcgcctct gatggttatt ccatcgaggt
     4321 tacgaaagag gagcttacgg gggggatcat tgcattaaag gatgccggtg aatggatcgt
     4381 tgaggtcagc cctgataaag gacccattcg catggttgcc cccttaacgc cggcaaaccg
     4441 ttgggtttac tcgattatgg aaattcaggt gaatcaataa tcatttgaaa gagcggcttt
     4501 cgaaacggaa aacgtgcggg atgtgtttga tcccgcacgt tccttaacct ggtctgaaac
     4561 gaaatgcttc cataatattg ggcagcgatt cgatgcgagt cagcacccgg ctgagttggg
     4621 tgatatcggt gatgtccagt gtcagattga tgaccgccag attattggaa agattgatct
     4681 tgatgtctcg caggttgata ccttcattgc taagaataga ggaaatatcg ttcatcagac
     4741 cctggcgatc atatgctttg atctgcacat ccaccggaaa cgttcgatgt gcctctcccc
     4801 aatttacttt gataatcctt tcatggtctt tgaccgaaag catattgggg caatcttgcc
     4861 ggtgaatggt aacacctcgc ccgcgcgtga tatagcccat gattgcatca cctggggcag
     4921 ggctgcagca gcgcgcaaaa tgtgtgagga taccctttaa tccaagaacg ttaacggtgt
     4981 cagcaccttc ttgttcggca accggcgcat gtaccttaaa gagagggtcc tcgttgtctt
     5041 tttcaacatc atccaatttg ctgatcaatt tgcgcagcga gagatctccg catccgaggg
     5101 aaatgtacaa ttcctcttcg tttttcagat cgagggaatg cgctaagttc tcgatattga
     5161 cgttttccaa acccaaacgg cgaaattctt tatccaggat actgcgaccc tggttgaggt
     5221 tctgctcctc atcctgtttt ttgaaccact gccgaatttt ggaacgcgca cgctgggttt
     5281 tgaccaatcc caggttgatg ttcaaccagt ctcgagaagg accgccatgt ttggaagtga
     5341 gtatttctaa ttgatctccg gtttgcagat ggtaatcgag agagacgagc tttccattca
     5401 cttttgcccc gcggcagcga ttgccgatct ctgtgtggac atgatacgcg aagtcaatgg
     5461 gtgttgatcc acggggaagg tcaataatat caccgcgcgg cgtgaagaca tatacgcgat
     5521 cttcaaaaac gtcgctcttc atgccgtcta caaattcctg agcgtcatcc acatcctgct
     5581 tccaatccat caactttcgc aaccagttta tccgctgctg atacgtgtct gaaaaaggct
     5641 ttttttcctt atacatccag tgggcagcaa tcccaaactc agcgttctgg ttcatttcag
     5701 acgtgcggat ctggacctcg agggtctttc catcggagta aatgacagcc gtgtgcaagg
     5761 agcggtaaaa attatccttg ggggcggcaa tataatcatc gaattcgttc ggaatggggc
     5821 gccagtgggt gtggatcaag ccaagagcgg cataacagga tgggatatca ggaacaatga
     5881 gccgcactcc gcgcagatcg cgcaccattt cgaagggcat gcccttgtcg gtcatttttc
     5941 tatatatgga atagatatgc tttgggcgac cgctgatctc cgcatcgatc tttccttttt
     6001 tcatcaccgc gtccaggtct tttataatga gcgcgatctc ctcttcacgg tctttccgac
     6061 ggtccgaaag tttttgcgcg atctctttat acatttccgg ttttatataa cgcagagaaa
     6121 gatcttccaa ttcccacttt agctgccaaa tgcccaggcg attggcaagc ggagcaaaga
     6181 tatccaacgt ctcctttgcg atactatagc gtttcttttc cggcatgtgg ctgagagtgc
     6241 gcatattgtg caaacgatct gccagcttaa tcacgaccac gcggatgtcg tcgcccatgg
     6301 cgagaagcat ttttcttaac gtctcggtag ccagttcatg ccggcggctg cgggcgtgtt
     6361 ttaaatctga atcttcaccg tcttcatcga tttcggcatt gccctgtgcg gacgttgctt
     6421 cttttttctc caccatctga tctgccctgg cgacacgggg gagattggtc agtttcgtaa
     6481 ccccatccac cagtttcgca atttcggcgc caaacttgtt ctccaaatct tccagggtga
     6541 cgggggtgtc ttctaccgta tcgtgcaaca gcgcagccgc gatcatttca ggtggaacct
     6601 gcaggtcggc aaggatctca gcgacagcca gacaatgggt aatataaggc tcgcctgaaa
     6661 gtcgtttttg tccttcatgg gctttttccg cgaatttata agcgcgcagg atcagatcgc
     6721 ggtctacaac cgtatatatt tcagggagct tggcgagcag cgtgtcaatt tccatagaag
     6781 aattagcaat tgattacgaa taagtataat cttcaattct ttaattcact catttaaatg
     6841 cctcaacttt caaaattttc aatcaattcg cgtaggttta tgtcgttaaa agaagcatag
     6901 attcgatctg ccttatccag aggcaacttt tgggtcaagg gattgggaac agcaatacaa
     6961 aatatgcccg cgttttttgc tgcctggata ccgctaggcg agtcttcaaa agcgataact
     7021 tctgaagggg gtaatctcca tttttccaag actaaatcat aaatttctgg atgtggtttg
     7081 gaatgtgaca cttgatcgcg cgtgcatacc ggatcaaaaa cgtgatccaa tttcaggttt
     7141 tgaaggtgtc cggagaccca atctctgtgt gaatttgaag caatagcgag cctcaggttc
     7201 agcgcttttc cggtttgaat ggtgttgacc acaccttcac gaggattgtc gaaaggaccg
     7261 gaggtaagcg tgaaatgatc gtataactcc agtgccaatc ggatctgatc attagacaaa
     7321 ctcatttcgc gcatgatatc ctgcacgaac cgattatcgg tggcgcttcc aatataatcc
     7381 aaataccgtt cgagagggaa ggtcaattga aaatggtcga acactttttc ccagatatgg
     7441 acttcagcgg tttccgtatc gagtatcaaa ccgtcgaaat cgaagatgat gcctttgatc
     7501 ttcatgatat tagaaaatgg cgtcggtcaa tcccaacgct tgttgaaggt aaagaatatc
     7561 tttctgtaaa ttcacattaa caggcacgcc ctgccttgcc acaatttttt ccatttcgaa
     7621 ttccttttca ccggcagtgt agatgcgagg cttgccggga gctttgcgag tgttccgcag
     7681 cgtgcgcagc aggtcgccaa tatttttctt gaattcattg acaggcaaga aattttcaat
     7741 gttcattgcc aggaagaaat gacccactcg aaaatgggtg ggttgattat tctcacccaa
     7801 accggaaagc tgaaataaaa aagcgccgct ttgaagggca gaggagagga tctcaacgat
     7861 ggttgaaagg caataaccct tgtggctgcc gttcagttca tccacccctc ccaggggaag
     7921 cagcgcggct ctatcgtgga cgatttcctg caagatctga tcaggatcgg tcatgggttt
     7981 gttatcctga tcgatcaccc aaccaacagg gagcgcttct tcggcgcgcg ccagaacttc
     8041 gatctttcca cgctgtgtaa tggaagtagc cccatcaaac aggaaagggc agtcttcatc
     8101 cgttggggct ccgaaactga taggattggt tcccagcgtg ggttgtgtgc catgcgttgg
     8161 agaaacacag gggcgagcat tggtgaagct cataccgatc atgtcttgct gtacagccat
     8221 cagtggataa aagccgtcga tgccaaaatg ggtagagttc cgcacagcca ccgcacccat
     8281 gccgaactct tgggctttgg tgattgccat ttgcatggat ttggtcgcaa ccaccatacc
     8341 gagtccatga ttgccgtcga ttactgcggt agtgggcgat tcgcgaacga ctgtcagcct
     8401 ggcttctttt tcaatcaaac cgctttttaa ccgctcataa tacatgcgca gtcttccaat
     8461 tccgtgcgat tcgatgccgc gcagatcgga ttttatgagc acgtcggcac acattctggc
     8521 atctgcctga ggaacgcccg cctttagaaa caccgcaagc ataaaatcgt acagttttgt
     8581 aacaggcacg tgaatagtat cttgttccat cgaagatcct tcatccaaat gatattggac
     8641 tattttagca tctctatgtg agatatggac cttgaaaggg aaaggttttg aagaacctgc
     8701 tgctgtgttt tgtccagcga tccacaaatt gttgctgcca gcaattcgcc aactcccgct
     8761 cccaacatga acccctgacc gcacatccca accgccagca ataagccagg caggtcagga
     8821 acaaaaccta gaaaaggtga accatctggt gtcatcgggt atagcccgcg ccaggtgcgt
     8881 ctgacgcgaa tattttgtag cctgggaatg agggcaagca agcgtttggc ggctatagga
     8941 aggaaatcag aggtactctg cgtatcgaaa ccctgcacag gaggttcggg ggagtaacaa
     9001 aaaagtacct gcccggtgga atgctgataa aaataaaagt tggatgtaac atctatgggg
     9061 cgcatatcta caatcaacgg atccaggaag atttggaccg gctcagtgat gccagcctca
     9121 tgggattcgg gtgaaacggg aatgtcaacc cctgccagtt tcgccatttc gcctgcccat
     9181 gcacccgcag cgttgatgac cactgcggtt gaatagtgcc cacgggtggt ctggacacct
     9241 acgatcttgc tgttgtgttt cgtaaaacca atgacagatt cattgaaatg aaattcagca
     9301 tggcgagcta cggcatgttt gtaaaatgca aacagtgaga ggagagggga agcatgcccg
     9361 tcatctggag aaaaggttcc gcccaaaagc ccctgtggat ttagatctgg aaccacatca
     9421 agcatagctt ttgaatccaa ccaatcgatg ttcaggttgc acgccttttg ctgaagcaaa
     9481 aggttctttg ggctgatctc atcttctttt cgatacactg cgtaaacgta cccacctttt
     9541 ttccactcga tctcatcacc gtacgtttct ttccagatgg agagaatttg aagactgcgc
     9601 tggcacaacc aggctttgga tggatcggaa tgcgtagcac ggatcccgcc gatagcggct
     9661 ttgttcgaaa cctgaccgat gcttgagcgg ttatccagaa caagaacttt taatccaact
     9721 ttcgataaag agaaggcggc tggcacgccg atagatcctg caccaatgat gatgacatca
     9781 tagtttgcgt taggcatgat cggaatcccc ttcacgagcg tctttttcga cgaatgttat
     9841 caatggaacc tccacaacca gaggtctttt cgaaaatgaa gtgatctcat ccttttgcac
     9901 accttcttct tgcatgatct gcaaaatcaa cgcttcgcag gtttttccgc cacaggcacc
     9961 catgccagct ctggtgatgg ctttaagttg gttgaggtcg tgcataccgc ctcgaatcaa
    10021 atcgcgcagc tctttttccg ataccctctc acagcgacaa atgattgtct cttgttcttc
    10081 ttgacccttg acccaatttg gggtgggcaa tggtggtatc ttttctaaat ggataccagc
    10141 gatccgctcg gctatctcgc ctggcgctac cagtttgagg agggaagtgt ggtccatttt
    10201 tgaagattga actgcttgaa cgacctctgc aacgccgatc acgctcccct cgcgatctgt
    10261 aacaggaaag gattgaccaa tgacatcgtc tttgttgatg tggaactcgc agggaagcgt
    10321 gacgatggga aagcgagcat ccttgcgata gtcgaccaga gtgattgcaa gaccggggca
    10381 gccaatcaca cattgggcac acccgttgca ctttccttcg ctgtcaaaga aatcagggat
    10441 cgagagaata tcatcgggtg gaatctgaat caggtgctga acacacaacc ccgaacacgg
    10501 gttgcatgga atttcctgca cacagtgaaa tacagggtga acttttgaag aggtgtggat
    10561 cgttttggag tcgcgaattg gtccgggttt ggaagaaaga acctgtttga agcgcataag
    10621 ttctgtgagc tctgcatcag agacactacc tttttggagt tgcgtcagga ttttctgtgc
    10681 agtgatcttg cccgaaacaa tagcagcaga tgcctcggct atctcctccg catcgccagc
    10741 cgcatgcact tgaaaaccaa actcctgtgc ttgttggaaa agttcattca ccgggctcaa
    10801 accagccgca ataagaattc catcacaagg aatagttttt tcggtgttcg gaatcggttg
    10861 aaagctttca tccacttcag caatggtgac tgattttacc tggtccgctc cattcgctct
    10921 cagaatggtg tgggatggaa agatgggcac acccaaccgc tttagtttat cgagatgaac
    10981 cttatagcca ccgcactgtg caagcagttc agccaacccg gcaatatgga tgccagcctg
    11041 gatagcatga taggctgcaa tcagccccac atttccgcca ccgaccacga aataagtctg
    11101 tccgggagtc acccagtcat ggttgagcag agtttggaac gcgccggctc ccaaaattcc
    11161 tgggagggag tttccggaaa aacgcaacga tttttctctt gctccggtgg caacaaccag
    11221 aatctccggg tcgatgagcg catattggcg attgtctttc aacacgccca ccttatgatc
    11281 gctgaaaact gcgactgccg gcgagttcag ccacatttct accgaagtgc agtgcgttgc
    11341 ttcgccagcc agttgtctgg caatgtcgat accgcgcgtg ccagcataca caaattcttt
    11401 tgaaccaaag aaacgatgcg tttgcagcac aagcttgccg ccggcttcgc tcttatcgtc
    11461 cacaagcagc gtttcgacgc catgttttcc caattcaatg gcggcggata agccagccgg
    11521 accagcgcct atgattaaca cttgaacgga tttacggata atggggtgtt gttcagcggg
    11581 gctgctctca ggaagggcgt aatgcccatt aagggatgca agttgtatcc cttcgcgaac
    11641 aggttccatg catgccttgc gcggaaaacc atccacaagg agcatgcatt gcgagcactg
    11701 accattcgca caaaagatgc cttgtggaaa atgatctttg tgatgatact gaaagacatg
    11761 gtatccgtta gcgatcaatg cggaggcaat gctttcattt tcatagccct cgcagggttg
    11821 cccattccag gtgaaattga ctttcttccc acggtttatg gggagaatgg gatgggattc
    11881 gatacgatgg ttgatttttt gcatatttgt atctgtatgt tgttggtatt ataaagaaca
    11941 attgaatcag aatatattga ggattaccag ttaataagat cataaaagta tatagaaaat
    12001 ggtatgctat gttaaaattt attgatctct agttctggag ttaggctatg aaaaagatag
    12061 cggtgttaac aagcggggga gatgctcccg gtatgaatgc agcgatccgt gctgttgtgc
    12121 gcacgggtat acagaaaggg atggaagtgt ttggagttcc ccttgggtat tccggattga
    12181 tcgatggcaa
//
LOCUS       DAOSDS010000062        11845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2085_length_11845_cov_5.935199, whole genome shotgun sequence.
ACCESSION   DAOSDS010000062 DAOSDS010000000
VERSION     DAOSDS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11845
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2085_length_11845_cov_5.935199"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="PLL88_07860"
     CDS             complement(<1..1001)
                     /locus_tag="PLL88_07860"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOJ01517.1"
                     /translation="MADFILEMHDIKKHFPGVQALKGVDFNVHQGEIHALVGQNGAGK
                     STLLKILAGIYIPEQGSIKVNGTELTKWNPQKIMDMGISFIYQELNLIQSMTVTQNLF
                     IGSESRNALGLTDWKKMRARAKDALARIGVMDIDVNVPIRDLTVAKQQLVAIARALAQ
                     NPKLLVLDEPTSRLGFEDSEKLFEILKRMQRENISIIYVSHRLSEIYRIADRVTVLRD
                     GKCIITDQTKAISAHELVKYMVGKDVQKNKFAQVKDHRNLLLQSTHLQGRANNVDLSI
                     YGGEVLGLVGTVGAGKTEFVRMLFGIESLSGGTIEVNGKKLKKITPTEAIANGMALCP
                     "
     gene            complement(1046..2125)
                     /locus_tag="PLL88_07865"
     CDS             complement(1046..2125)
                     /locus_tag="PLL88_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOJ01518.1"
                     /translation="MKRKILFLITVTLLTAMFLTACKSAAPAEESSVVDAEAPANEAV
                     AKETTSSGVKTIGLASFQLGNDWNIQAAEGVKAKAAELGWEVIQTNAESNSDAQLTAL
                     EGFLSQGVDAVVIPGGSGPALEPIIKQFKDAGIPVVTIDLTSLNATTNIYPDNYMSTE
                     LLAVFAVNKMEAVPGKYAHLTVPNMGWKTVDIRDKVADLAFEIEGWQSTGILDSGIAD
                     AVNQSITATRSALLANPDLNLIYSSWGMPAVGAARAIREAQLQDSVFVVCTDADRIVL
                     AEMAEEDSPIAAVIGQRPFDMGTMAVDYLVKAFDGDADIPTITYAPFYFVTKEPQLLP
                     PGVEVLNPEEAWNALYPNVEFGKIN"
     gene            2682..3446
                     /locus_tag="PLL88_07870"
     CDS             2682..3446
                     /locus_tag="PLL88_07870"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ01519.1"
                     /translation="MSSQDVHAEKQHHIDMQQGDVASTVLIPGDPGRVALFASLMDEA
                     SKIAEKREYITYTGTKDGVPISCTSTGLGCPPTAIGVEELIRIGAKNIIRIGTCGAIQ
                     SFLEPGHMIIVTGAVRGEHTSEEFINMEYPAVADYRIVRALIDACEKLKLPYHLGIVR
                     SHDAFYLESPWALGDYRKRLQPWVDMGVLALENESSTIFVVSSMQGIRAGTILASGYS
                     ILEEHEEKIHEEENIRKMVSAAIEAAKILHERGLAN"
     gene            3459..4202
                     /locus_tag="PLL88_07875"
     CDS             3459..4202
                     /locus_tag="PLL88_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ01520.1"
                     /translation="MEKQHHIDLQPGDVAETVFLPGDVHRAKFIADLFDEAKEVAHKR
                     QYITYTGTYKGVKVSVTSTGIGCPALAIAVEELINVGAKNFIRIGTCGALQRHVRVGS
                     IVIATGAVRGDGTSREYFPLEYPAVADFDTLEALLEAAKERKVEPNVGIIRAHDAFYA
                     ESILAGGDYLLKDKPWIESGVLSTENESSTLFTLCTVRKCRSGTILVPVGHHLFPEEI
                     ISTEQLTDAIKLETLIALDAAVKLKQKFG"
     gene            4221..5492
                     /locus_tag="PLL88_07880"
     CDS             4221..5492
                     /locus_tag="PLL88_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ01521.1"
                     /translation="MKQTLAQYQGQIEKNYRWNFFWMAVDNMMFFFIFMGLSPYTVLP
                     YYVQQFSSSSILIGFVPAIYILGNTLPQPLMARFLAGKIERKKYLVVAAFLQRFGILG
                     FLLLAIIQPLLGLSNVLTLVLFFAMLVLQNTASGFYVPAWIDFMGRAIPRKRGILFGI
                     SNFAGGLLGIGMGALLTYLLGAYPYQQAIQVIFGISFLASMISFGAILSWREVLPPQA
                     MVKEVKAETHNKPQKNKNFATYLVFRGLMVILEIGTSYYTIVALQTGNITAAQIGIFT
                     TIMSLAETMVNPLFGWLGDKKGFLNVVKISAVMGIAATLMAANLTTLTAFYAIFILAG
                     VMVSGFQITNFNMIYEFSPADQIPAYTAASQIALSPLSGVMPFIGGALVGAFGYTSVF
                     WLSGLVGMTGLLGMSFLVRNPKIKRDVQRSI"
     gene            5492..6256
                     /locus_tag="PLL88_07885"
     CDS             5492..6256
                     /locus_tag="PLL88_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ01522.1"
                     /translation="MSSQDVRAEKQHHIDMKKGDVAPTVLIPGEPQRVEVFASLMDEA
                     KKVAEKREYITYTGKKDGVAVSCTSTGLGCPPTAIGVEELIRIGAKNIIRIGTCGVIQ
                     PYIKPGEFIIATGAVRGEHTSEEFIDMEYPAVADYRVVRALIDACEKLKMPYHLGIVR
                     THDAYYLESPQARGDYMARLQPWIDMGVLALENESSAIFVIAGMQGIRAGTILTCGNP
                     IYTPNADDCDWDSNIVKMVEAGIEAAKILAEHDLDQ"
     gene            6316..7641
                     /locus_tag="PLL88_07890"
     CDS             6316..7641
                     /locus_tag="PLL88_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ01523.1"
                     /translation="MKTLLKNGISFYPPTYEVKPINILIEDDEIINVSEKPMEEQLPD
                     TVVYDLKGNLVVPGFLDCHTHLAQSFGRGIYDNLHLTQWLVVMSTHFDKLTEEEAYKA
                     TFLGCIEALKSGTTTVAEMATAGPHLDACIQAIADSGLRADVCTAVGDFQEGENPPPE
                     LSTSQVLDVMRTMHKRWHGTYDGRITVRVSPVGLPACTEELMRGSRDLANELGVGIHT
                     HCSEGETETQNAYERFNCSEVEALERFGVLGPDCQLVHDIWLTEHDKQLIAEYDCSAV
                     TCPSTNTKITDGMPPMPDLYKLGVNIAIGCDGEASSGTYDMLQEARLVSLLGKVRTME
                     ADMFPAETVYEMMTVNGRRAIGFNTKVGEIKPGFKADLTVIQYPTPHMIDERRLLSNL
                     IFSASGADVLSVFVDGKPLMWQRELLHIDEEKLVADILATMRKAEHLLR"
     gene            7765..8817
                     /locus_tag="PLL88_07895"
     CDS             7765..8817
                     /locus_tag="PLL88_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ01524.1"
                     /translation="MNKKLYVVLALVLVATLVFTACAPKEEAAPAEEAAAEDTFKVAM
                     LTDLGGLAASEEVKGFSDLGWDALKQAEAELGVEITLLEAKELADLEPNLIKMANEGY
                     DVVVGVGFLFTDAMSAVAPQFPDTKFIIVDSVVDEPNIASLVFAEEQGSFLVGAIAAG
                     TSQTGTIGFIGGMESALIEKFEAGYIAGARAINPDIVVLSGYTGNFTDVAAGKELTLT
                     QYGQGADVVYAAAGNCGLGTIEAAEQEDLYAIGVDTNQDGIAPGFVLTSMLKRVEVAV
                     FNAIKGAYEGNFVAGITTYDLAAGGVGYSPMEYTKDAVPAGLLDTVNALAERIISGEI
                     VVPTTVEEANTFVLPE"
     gene            8886..10412
                     /locus_tag="PLL88_07900"
     CDS             8886..10412
                     /locus_tag="PLL88_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01525.1"
                     /translation="MAYILELRDICKHFGNVLANDHINLKVEKGTVHSIVGENGAGKT
                     TLMNILYGLYKQTTGEIFVDGTEVDIESPSDAIDLGIGMIHQHFKLIPAMTVAENVML
                     GIEPTKGKVFLDLKKINGSTLEISNKYGLAVNPKSMVADISVGIRQRVEILKALYRNA
                     NILILDEPTALLTPQESEDLFRIIERLKKDGKTILFISHKLKEIMQISDVITVLRDGK
                     VVDTVKKANTDEVHLARLMVGRDVFLKPAPKPKKIGEPILRVENLSAVNEYHHPVLTN
                     VTFDVCSGEILGIAGVDGNGQTELVRALIGLQPITQGKISLKGTEITHLSVKKMRSAG
                     IGVIPEDRLQEGLVEDFSVEENLFLGLQRNQPFSNGNKLDWNAVHKNADSLVKTFDIR
                     TPNIEHKARLLSGGNQQKIIVAREIYHAPDTIIAAQPTRGLDIAATDFVHEQLIKQRD
                     SGKAVLLLSLSLDEIMLLSTRIAVIYKGTIVAVVNKDAVTPEDLGLMMLGGSYEHISA
                     "
     gene            10429..11508
                     /locus_tag="PLL88_07905"
     CDS             10429..11508
                     /locus_tag="PLL88_07905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01526.1"
                     /translation="MLGIRKFFLKRQPVFITIIAVVLAFFVVGLILAATGRNPFNAVY
                     YFVRGAFGNVMNTTDTISRVIPLTIAGIAFLVGAQSSVFNVGIEGQLLLGAMASAIVG
                     YIVKLPFYLHLPLMLLAGALVGGLYGLIPAWLKTRRKVNEILSTIMLNYPATYLTHFL
                     VLKVIPIEGVVPATPFIENSARLPLLIPGSQLHAGVFIMILVVIFAYYLLYKTSLGYE
                     MRAVGYNRVAARFHGIPVEKRMNFAFFLSGALAGLAGAVEVAGVHYRFLDQFSPGYGF
                     DSITVALVGLLNPFGVIISATLFGALKTGILEMSVYSGIPRQLTTLINGIVVLFVAAK
                     DILGGWYARIFRGKDTYEAEKEISQ"
     gene            11505..>11845
                     /locus_tag="PLL88_07910"
     CDS             11505..>11845
                     /locus_tag="PLL88_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01527.1"
                     /translation="MNLNWADALKAIFTLTLLTSTIRVSISILLAGMGEMLTERSGVL
                     NIGVEGTMLMGALAAALGSYFTGSPWIGLIAAGLTGMLIGLLFSFIAITLRSNQSITG
                     LAIYLLCVGLSG"
BASE COUNT         3000 a         2762 c         2980 g         3103 t
ORIGIN      
        1 gggcagagag ccatgccgtt tgcgatagcc tccgtggggg tgattttttt gagctttttt
       61 ccattgactt caatggtccc ccccgagagc gattcaatgc cgaatagcat acgcacaaat
      121 tcggtttttc cggctccaac cgtacccacc agccccaaaa cctctccgcc ataaatggag
      181 aggtcaacat tatttgccct tccctgaagg tgagtggatt gcagcaaaag attgcggtga
      241 tctttcactt gggcgaattt gttcttttga acatctttgc cgaccatata tttgaccaat
      301 tcatgggctg agatggcttt ggtctgatcg gtaatgatgc attttccgtc gcgcagcact
      361 gtaacgcgat cggcgatgcg atagatttcg cttaagcggt gcgatacata gatgatgcta
      421 atgttttccc tttgcattct ttttaatatt tcaaagagct tttcggagtc ttcaaacccc
      481 aaacgactgg taggttcatc tagaaccaac aacttgggat tttgtgccaa tgccctggca
      541 atcgctacga gctgctgttt ggcaaccgtt agatctctta tcgggacgtt cacatcaata
      601 tccataacac cgatgcgagc cagggcatct ttggctctgg ctcgcatttt cttccaatcg
      661 gtcaatccaa gtgcattgcg agattcacta ccgataaaca ggttttgggt aacagtcatg
      721 gattggatta aatttaattc ttgatagata aaactgattc ccatatccat gattttttgt
      781 ggattccatt tggtcagttc tgttccattc actttgatcg atccctgttc ggggatgtag
      841 atccctgcca gaatctttag cagagtgctt tttccagccc cattttgccc gaccagagca
      901 tgaatttcac cttggtgaac attaaaatcg acacctttta aagcttgaac gcctggaaag
      961 tgttttttaa tatcatgcat ttcaagaatg aagtcagcca cgatagtttc tccttcaatg
     1021 ccttggactt ttggtaaaaa tcttgttaat ttatcttgcc aaattcaacg tttggataca
     1081 aagcattcca tgcttcttct gggttaagta cttcgacccc cggaggcagg agctgtggtt
     1141 ctttggttac gaaatagaaa ggcgcatagg tgatggtagg aatgtcagca tcgccatcaa
     1201 acgcttttac aaggtaatcc acagccatcg tacccatgtc gaatggacgc tgtccgatta
     1261 ctgctgcgat gggagaatct tcttctgcca tttcagccag cacaattctg tcagcgtctg
     1321 tacaaacgac aaatactgag tcttgcagtt gggcttcacg aatcgcacga gctgctccta
     1381 ccgctggcat accccatgag gaatagataa ggttcagatc gggatttgca agtaacgcag
     1441 agcgggttgc ggtaatagat tggttaacgg catcagcaat acctgaatca aggatgccag
     1501 tggattgcca tccttcgatc tcaaaagcca aatcggcaac tttatcgcga atgtcaaccg
     1561 tcttccaacc catgttaggg actgtcaggt gtgcatattt cccggggact gcttccattt
     1621 tatttactgc gaagactgcc aacaattcgg ttgacatata gttatcaggg taaatattgg
     1681 tcgttgcgtt caaggaagtg aggtcaatag taaccacggg gatgcccgca tctttgaact
     1741 gcttgataat gggttccagc gcaggtcctg aaccaccggg aatgacaaca gcatccaccc
     1801 cctgacttaa aaaaccctcc aatgccgtca attgtgcgtc agagttagat tccgcattgg
     1861 tttggatgac ttcccaacct aattcagcag ctttggcttt cacaccctcg gctgcttgaa
     1921 tgttccaatc attgcctaac tgaaaactgg ctaagccgat ggtctttaca ccacttgatg
     1981 tggtttcttt tgccaccgcc tcatttgctg gcgcttcagc atccaccaca gaagattctt
     2041 cagcaggagc agcgctcttg caggctgtta aaaacatggc agtaagcaag gttacggtaa
     2101 tgagaaatag aatttttctc ttcatgaaat tctccttaag ttttttattt tttgctgcat
     2161 tagattattg gatattgaac catcggcacc tccgcaaatc gaaaagttta tagataaatt
     2221 attgcatata tacgtctaga cgtatatata atataagaca aacatctttt cgtcaagatt
     2281 tatcgagaac taatgttttg agggggtaca tggcagcaga attctcaagg tggttaagac
     2341 cacttgacat ggatttcaat tcatattatg atggaaatcg ttgatgaagc gggatagggg
     2401 ccgtattggt gttatacgcc gtcttgatcg acaataatga gagtatctca aaattgttga
     2461 gagagaggaa tggttccatt gcaatggctg cctgttttgc aataaccgca aagggttttt
     2521 ttctgtcgcg tttctttaac tctgtaaaat ccccccacgt aatctatcgc tggcacgcag
     2581 tctggagata atctctttga gcacccgtat cgggtaaaat gcgcctccat gtcaaattca
     2641 cacggagaaa aggtttcgca ttccagttta aaggagagaa gatgtcaagt caggatgtgc
     2701 atgcagaaaa acaacaccat attgatatgc aacaagggga tgtagcctca accgtcctca
     2761 ttcccggcga tccgggcagg gtcgcgctgt ttgcctcgct catggatgag gcaagcaaaa
     2821 ttgccgaaaa aagagaatat atcacctaca cgggtaccaa ggatggcgtg ccgatcagtt
     2881 gcaccagcac cggattgggt tgccctccca ctgccatcgg cgtcgaagaa ctgatccgca
     2941 ttggcgccaa gaatatcatc cgcataggaa catgtggagc gattcagtcg tttctggagc
     3001 ctggacacat gatcattgtt accggtgcag tgcgcggtga gcataccagc gaagaattca
     3061 tcaacatgga atatcccgcg gtggctgact accgcatagt gcgcgcattg atcgacgcct
     3121 gtgaaaaatt aaaactcccc tatcacctgg gtatcgtacg ttcgcatgat gcattttatc
     3181 ttgagtctcc ctgggcgtta ggtgattacc gtaaacgcct gcagccttgg gtcgatatgg
     3241 gtgtgctggc gcttgaaaat gaatcatcga ccatattcgt cgtttcaagt atgcagggca
     3301 ttcgcgccgg aacgattctg gcgagcggat actccatcct tgaagaacac gaagaaaaaa
     3361 tccacgaaga agaaaatatc cgcaagatgg tctcggctgc tatcgaagct gccaaaatcc
     3421 tgcacgagcg cggattggca aactagaaag gatctaccat ggaaaaacaa caccatattg
     3481 atttacaacc gggtgatgtc gccgaaacag tttttttgcc tggtgatgtc catcgtgcaa
     3541 agttcattgc tgacctcttt gacgaagcca aggaggttgc ccataaacgc caatacatca
     3601 cctatacggg cacgtataaa ggtgtaaaag tgagcgtaac gtcaacaggc attggttgcc
     3661 ccgcgcttgc cattgcggtc gaggaattga tcaacgttgg tgcgaagaat tttatccgca
     3721 ttggaacctg tggtgccctg caaaggcatg tccgcgtcgg cagtattgtg atcgcgaccg
     3781 gtgcggtgcg cggtgatggg accagccgcg aatactttcc gctcgaatat ccagcggtgg
     3841 ctgattttga cacgctggaa gcgctgttgg aagcggcgaa ggaacgcaag gtagaaccca
     3901 acgtaggcat catccgtgcc cacgatgcat tctacgccga atccattctg gcgggcggag
     3961 attatttatt gaaagacaaa ccgtggatcg agagcggcgt gctttcaacc gaaaacgaat
     4021 cttccacatt gttcactttg tgcacagtgc gcaaatgccg cagcggcacg atccttgttc
     4081 cggtcgggca tcatttgttc cccgaagaga tcatctccac cgaacagttg acggatgcga
     4141 tcaagctcga aacgctgatc gccctggatg cagcggtaaa gctcaaacaa aagtttgggt
     4201 agggttatca ggtagctttc atgaaacaaa ctttggctca ataccagggg cagatcgaga
     4261 agaattatcg ctggaatttc ttctggatgg ctgtggacaa catgatgttc ttctttatct
     4321 tcatgggtct ctcgccgtac accgttctgc cctactatgt gcaacaattc agctcatcca
     4381 gcatcctgat aggattcgta cctgccatct atattctggg aaataccctg ccccagccac
     4441 tcatggcgcg ctttttggcg ggcaagatag aaagaaagaa ataccttgtg gttgccgcct
     4501 tcttgcagcg ttttgggatc ttgggttttc tcttgctggc gatcatccag cctctgctgg
     4561 gattgtcgaa tgttttgacg ttggtgctct tttttgccat gttggtcttg cagaacactg
     4621 ccagcggttt ttatgtgccc gcctggattg atttcatggg cagagccata ccgcggaagc
     4681 gaggcatact cttcggcatt tccaatttcg ctggtggtct gttggggatc ggcatgggcg
     4741 cgttactgac ctatttgctc ggtgcgtatc catatcagca agcgatccag gtgattttcg
     4801 ggatcagttt tctggcttcg atgatctctt ttggggcgat cttatcctgg cgagaggttc
     4861 tcccgcccca ggcgatggtc aaagaggtaa aagcagaaac acataataaa ccacaaaaaa
     4921 ataagaactt tgccacttat ctcgtttttc gaggattgat ggtgattctg gagatcggca
     4981 cttcgtatta caccatcgtt gccttgcaaa ccggcaatat caccgcggca cagattggca
     5041 tctttacaac catcatgtcg ctggctgaaa cgatggtgaa cccgttgttt ggctggctgg
     5101 gtgataaaaa aggtttcctc aatgtggtga agatctccgc agtgatggga atcgcagcca
     5161 cgctgatggc tgccaatttg accacgttaa ctgcgttcta tgcgatcttc attttggcgg
     5221 gcgtcatggt cagcggcttt cagatcacga acttcaacat gatctatgag tttagtccgg
     5281 cagatcagat acctgcttac actgctgcca gccagatcgc cctctctccg ttatcgggag
     5341 tgatgccatt catcggcggt gccctggttg gcgcattcgg atataccagt gtgttctggt
     5401 tatctggttt ggtcggcatg accggcttat tgggcatgtc gttcctggtc aggaacccga
     5461 agataaagcg cgatgtacaa aggagtattt gatgtctagt caagatgttc gtgcagaaaa
     5521 acaacatcat attgatatga aaaaagggga tgtagccccg accgttctga ttcccggtga
     5581 gccacagcgt gtggaagtgt tcgcgagttt gatggatgag gcgaaaaagg tggctgagaa
     5641 acgggaatat atcacctata cagggaaaaa agacggcgtt gcggttagct gcacaagcac
     5701 cggcttgggt tgccctccca ccgctattgg agtggaagaa ttgatccgca ttggtgcaaa
     5761 gaatattatc cgcattggca cgtgtggcgt tattcaaccg tatatcaaac cgggcgaatt
     5821 catcatcgcc accggcgccg tgcgcggaga acacaccagc gaagaattta tcgacatgga
     5881 gtatcccgcc gttgccgact acagagtggt gcgcgcactg atcgatgcct gtgagaagtt
     5941 gaagatgccc taccacctgg gaatcgtgcg cacccatgac gcgtattatc tggaatctcc
     6001 ccaggcacgc ggcgattata tggctcgcct tcagccctgg atcgatatgg gtgttttagc
     6061 gctggagaat gagtcttcgg ctattttcgt gattgcggga atgcagggca ttcgtgccgg
     6121 tacgatcttg acctgtggga accccattta cacccccaat gcagacgact gtgattggga
     6181 tagcaatatt gtgaagatgg ttgaagccgg cattgaagcg gcgaagatat tggcagaaca
     6241 tgatctggat caataaatta ggaaatatgg atcattggat ggttgtctta attttagaaa
     6301 gcagatgagg ctagtatgaa aacactgtta aagaacggta tcagttttta tccaccgacc
     6361 tatgaggtaa aaccgatcaa cattctgatc gaggatgatg aaatcatcaa cgtcagtgaa
     6421 aaacccatgg aagaacaact tccagacact gttgtgtacg acttgaaagg caacctggtt
     6481 gtgccaggtt tccttgattg ccatacccac ctggctcaat cgtttgggcg cggcatctat
     6541 gataatttac atctgaccca atggctggtg gtcatgagca cccattttga caagcttacc
     6601 gaagaagaag cctataaagc cacgttctta ggatgcatcg aggcgctcaa atcgggtaca
     6661 accaccgttg ctgaaatggc aacagccgga ccgcatttgg atgcatgcat ccaggcgatc
     6721 gccgatagcg gattgcgcgc agatgtttgc acagccgttg gcgatttcca ggaaggtgaa
     6781 aatcctcccc cagaactctc gaccagtcag gttttggatg tcatgcgcac tatgcacaaa
     6841 agatggcacg gcacatacga tgggcgcatc acggtccgcg tcagcccggt cggattgccc
     6901 gcctgcactg aagagctgat gcgcgggtca cgcgatctgg ctaatgagtt gggggttggc
     6961 atccataccc actgcagtga aggcgagacg gaaacacaaa atgcgtatga acggttcaac
     7021 tgcagcgaag tagaagcgct agaacgtttt ggcgttttgg gacccgattg ccagttggtt
     7081 catgatatct ggttgaccga acatgataaa cagctcattg ctgaatatga ttgcagtgcg
     7141 gttacctgcc cctcaaccaa caccaagatc accgatggta tgccccccat gcccgatctg
     7201 tataaactgg gcgtaaatat tgccattggc tgtgacggag aggcgagcag cggtacgtat
     7261 gacatgctac aggaagccag actggtttcc ctgctgggaa aagtgcgcac catggaagca
     7321 gatatgttcc cggctgaaac ggtatatgaa atgatgaccg tcaacgggcg gagagcgatc
     7381 gggttcaata ccaaagttgg tgaaataaaa cctggattca aggctgacct cacggtgatt
     7441 caatatccga caccccacat gatcgatgaa cgccgtttat tgtcaaacct gatcttctcc
     7501 gccagcgggg cggatgtcct ttcggttttc gtagatggca aacccctgat gtggcagcgt
     7561 gaactgctgc atattgatga agagaagctt gtggctgaca ttttagcgac aatgcggaaa
     7621 gctgaacatt tgttaaggta gacgcttcac agtgaaaaca ggaagccaga atataattac
     7681 acaggaggag gtggtagaca ctaaaaatat atgaaaactg gttagtgtta aacaacgttt
     7741 tacaaaaatt taaggagaag agtaatgaac aaaaaattgt atgtggtact tgctctcgtt
     7801 ttggttgcga ccttagtctt caccgcttgc gcacccaaag aggaagctgc acctgctgaa
     7861 gaagctgctg cagaggatac tttcaaagtt gccatgctca ccgacctcgg tggtctggca
     7921 gccagtgaag aagtcaaggg tttctccgat cttggttggg atgctctcaa acaggcagaa
     7981 gctgagctcg gcgttgagat caccctgctg gaagccaaag aattggctga cctggagccc
     8041 aacctgatca agatggctaa cgaaggttac gatgtggtcg tgggtgttgg cttcctgttc
     8101 accgacgcga tgagcgcggt tgctccccaa ttccccgaca ccaagttcat catcgtcgac
     8161 agcgtagttg atgaacctaa tatcgcttcc ttggtcttcg ctgaagaaca gggttccttc
     8221 ctggttggcg ctatcgccgc cggaacgagc cagactggta ccattggctt tattggtggc
     8281 atggaatcag cactgatcga gaaattcgaa gccggctaca tcgctggcgc ccgcgcgatc
     8341 aatcctgata tcgttgtgct ttccggttac actggcaatt tcaccgatgt tgctgccggt
     8401 aaagaactca cactgaccca atacggtcag ggtgccgatg tcgtctacgc agccgctgga
     8461 aattgcggtt tgggcacgat cgaagctgct gaacaagaag atctctacgc gatcggtgtg
     8521 gataccaatc aagacggtat cgctcccggt tttgtgttga cctccatgct caagcgtgtc
     8581 gaagttgccg tgttcaacgc catcaagggt gcctacgaag gcaattttgt cgctggcatt
     8641 accacctacg atctggcagc cggtggtgtt ggttacagcc ccatggaata caccaaagac
     8701 gctgtaccag ccggtctgct tgacacagtc aatgcacttg cagagcgcat tatcagtggc
     8761 gagatcgttg tgccgacaac cgtcgaagaa gccaacacat ttgtgcttcc tgaataagta
     8821 gtcgaaacaa gcaaggtggg agggttgccc tcccactttg caatttacca gttactggag
     8881 gaagaatggc ctatatcctt gagctgcgcg atatctgtaa gcatttcggt aatgtgctcg
     8941 caaatgacca tatcaatttg aaggttgaaa aaggaactgt gcacagcatt gttggtgaaa
     9001 atggcgcagg aaaaacaacc ttgatgaata ttctctatgg attgtacaag cagaccacgg
     9061 gtgaaatttt tgttgatgga accgaagtcg atattgagag cccatcagat gcgattgatc
     9121 ttggcatcgg aatgatccac cagcatttta agctcattcc cgctatgacc gttgcagaaa
     9181 atgtgatgct gggaatagaa cctactaagg gaaaagtttt tctcgatctg aaaaagatca
     9241 acggatcgac gcttgaaatt tctaataaat acggtctggc agtaaatcca aaatcgatgg
     9301 tcgccgatat ctctgtgggt atccgacaga gggtcgaaat tttaaaagca ctttaccgga
     9361 atgctaatat cctgattctc gatgaaccaa ccgcgctact gacacctcag gaatcggaag
     9421 atttgttccg catcatcgaa cggttgaaaa aagacggcaa aacgattctc tttatttcac
     9481 acaaattaaa agaaatcatg cagatctccg atgtcatcac tgttttacgc gatggaaaag
     9541 ttgtcgatac cgtcaagaaa gccaacacgg atgaagtgca cctcgcacgt ttgatggttg
     9601 gacgcgacgt gttcttgaaa cctgctccga aacccaaaaa gatcggtgaa ccaattttaa
     9661 gagtagaaaa cctctctgca gtcaatgaat atcatcatcc ggttctcacc aatgtgacgt
     9721 ttgacgtatg ctcaggcgaa atattaggaa tcgcaggagt ggatgggaac gggcaaaccg
     9781 aactggtgag agctctgatc ggtttgcagc caataacgca agggaaaatc tctttgaagg
     9841 gtaccgagat cactcattta tcggtaaaga agatgcgctc agccgggatc ggcgttattc
     9901 ctgaagaccg gctgcaggaa ggattggtcg aagattttag cgttgaagaa aacctgttcc
     9961 ttggattgca acgaaatcag ccattttcga atggcaacaa gctagattgg aacgcggttc
    10021 ataagaacgc tgacagtctt gtgaaaacct ttgacatacg tacgccaaat atcgaacata
    10081 aagctcgcct gctatcgggt ggaaaccagc aaaaaatcat cgtggctcgc gagatctatc
    10141 acgcgcccga tacgatcata gccgcacagc ccacccgtgg gttggatatt gccgctacag
    10201 atttcgttca tgagcaattg atcaaacaac gcgattcagg gaaagccgta ttgctgctat
    10261 ctctttcatt ggatgagatc atgctgctct caacacgcat tgcggtcatt tataaaggca
    10321 cgatcgtcgc ggtcgtcaac aaagacgccg tcacccccga agaccttggt ttgatgatgt
    10381 tgggcggatc ttacgagcat atttccgcat agacgaaagg aaacgacaat gcttggtatt
    10441 cgaaaattct ttttgaaacg acagcccgtt tttatcacga tcatcgcagt agtgctggct
    10501 ttttttgtgg tgggtcttat tctggctgca actggtcgga atccgttcaa tgccgtctac
    10561 tattttgtgc gcggtgcatt cggaaatgtg atgaacacca ccgatacgat ctccagagtt
    10621 atcccgctga ccatcgccgg tatcgcattt ttggttggcg cgcagagttc tgttttcaat
    10681 gtgggtattg aagggcagtt gctgctgggg gcaatggctt cggcgattgt gggttacatc
    10741 gtgaaacttc ccttttacct tcacctgccc ttgatgctct tagcgggcgc ccttgtgggc
    10801 ggactgtatg gtttgatccc agcctggttg aaaacaagac gcaaagtgaa cgaaattctg
    10861 agcaccatca tgctcaatta cccggctacc tacctgaccc attttctggt attgaaggtc
    10921 attcccattg aaggtgttgt gccagccact cccttcattg aaaattctgc cagactgccc
    10981 cttctcattc ctgggtcaca attacacgct ggggttttca tcatgattct ggttgtcatc
    11041 tttgcttact acctgctgta taagacctca ctcggttacg agatgcgcgc agttggctac
    11101 aaccgggtgg cagcgcgttt ccatggtatt cctgtagaaa agcgcatgaa cttcgcattc
    11161 ttcttgagcg gcgcgctggc tggtttggct ggtgctgttg aggtggcggg cgttcactat
    11221 cgattcctcg atcaattttc gcctgggtat ggtttcgatt ccattacggt ggctctggta
    11281 gggttgttga atccgtttgg ggtgatcatc tcagcgacct tgttcggcgc tctgaagaca
    11341 ggtattttgg aaatgtcggt gtactccgga attccccgcc aattgaccac gctcatcaac
    11401 ggtatcgttg tactctttgt cgcagcaaaa gatattctgg gtggatggta tgccagaatt
    11461 ttccgcggaa aagacaccta tgaagcagaa aaggagatat cacaatgaac ttgaattggg
    11521 ctgatgcatt aaaagcgatc ttcacgttaa ccctgcttac atcaaccatt cgggttagca
    11581 tttcgattct attggcagga atgggtgaga tgctcaccga acggtcgggg gtgttgaaca
    11641 ttggcgtgga aggaaccatg ctgatgggtg ccctggcagc cgcattgggt tcttacttca
    11701 ccggaagccc ctggatcggg ctgatcgcgg caggattgac gggaatgctg atcgggctac
    11761 tcttttcatt catcgccatc acacttcgca gcaaccaatc catcaccggt ttggctattt
    11821 atctgctctg tgttggtcta tcagg
//
LOCUS       DAOSDS010000063        11842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2087_length_11842_cov_4.624332, whole genome shotgun sequence.
ACCESSION   DAOSDS010000063 DAOSDS010000000
VERSION     DAOSDS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11842
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2087_length_11842_cov_4.624332"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="PLL88_07915"
     CDS             <1..438
                     /locus_tag="PLL88_07915"
                     /inference="COORDINATES: protein motif:HMM:NF021500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HOJ01528.1"
                     /translation="AVWAEGWYPQVLAPDATTGEAMNTNTEIKILVDPAANKVTLRVP
                     RSVFGDSSPDSWAYAAVVLSQEGYPSTGVWRVRDVSKNAEQWRLGGAPGGNNHTRVVD
                     MVWAQGNTPDQAALLSNFTPNDKPQAELSLEDFALIPMFRLQD"
     gene            complement(465..1778)
                     /locus_tag="PLL88_07920"
     CDS             complement(465..1778)
                     /locus_tag="PLL88_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ01529.1"
                     /translation="MKNRTGLRNVVIYEIYVRNHSQQGNFQAVVNDLLRIKALGVDYI
                     WLMPIHPIGKVNRKGSLGSSYAIADFMAVNPEYGTEKDFQNLIKAAHELGLKVMIDVV
                     YNHTSPDSVLAREHKEWFRLDRQGNPTTSVPDWTDIVELDHAHTELHDHLVKAIQKWT
                     SFGLDGFRCDVASLVPMEFWLKADAAAKAINPDIIWLAESVHTGWVAERRSKGLFAQS
                     DSELYKVFDLTYDYDVFRPWERAVKGTLDTRYYLDLLVLQDAIYPQDYVKLRYVENHD
                     TERVMAIAPSREQALAWTAFQAFNKGAWLIYAGEEAAAARKPDQFEKDVIPWSNYELT
                     DFIRNLAALKKDAAQKEGELCWLTSEPAVQAVWFTEKTSLAGVFNTEGKKGEILTAFP
                     DGTYRDLLSGEDFGIVNHTMQIPVSACIFHCQMKELPPQREMLLV"
     gene            1978..2316
                     /locus_tag="PLL88_07925"
     CDS             1978..2316
                     /locus_tag="PLL88_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HOJ01530.1"
                     /translation="MSEESRSIQFATMTILHAGNARKYISDAYKELSEFHFEAARKKI
                     EQAREEALFAHREQSKIIHAESDGESLEFSLLLTHAQDTLMTAVSEMHIAKNLINIVE
                     KIQEDHQKHA"
     gene            complement(2351..2995)
                     /locus_tag="PLL88_07930"
     CDS             complement(2351..2995)
                     /locus_tag="PLL88_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ01531.1"
                     /translation="MDNTNQDYSLRGEQTRKELIDAACDLFILNGFHATTTRQITDKL
                     GLVAGTLYNHFDSKITLFEAVLDTYHPWLRIPEVLRNASGKTVEEYIRDASEKLLREW
                     DKRPELIRLHLIEVLEFNGQHIPALFEKTFTTVSKTIEEVRAKDEKLKDVNVPLLYRA
                     VLGLFFGYLMSDKAIVDKENKFKESSFIEGGFDYFADAYLYGLFNQNINQPGKK"
     gene            complement(3331..4614)
                     /locus_tag="PLL88_07935"
     CDS             complement(3331..4614)
                     /locus_tag="PLL88_07935"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOJ01532.1"
                     /translation="MAQEKKWFERAFVQPMSRFANFRIIKSISAGFQSGVSILMVGAM
                     LSVLSIVLGWLPAVSASAFLTKFNAVKDLVFGVTGLVFTYGIAAANAKLNKTDQQAVG
                     FLAIAVFFIFMKPSITVDENYTSVFTTPFARFGMQAVFVSMVAGIWAGEVSAFFKRHG
                     WIMNSEGVPDIAKVWFENLIAGTFIVLSAWVVADVLNINLHTIFNNLLTPLMGILGSF
                     GGWILVVSLPPLLFYFGIHPASIMSLVGPVYYAALANNVQLLAAGLTPTVANGFFVVN
                     MGTLFLMNIGGAGATLGLNILMLFSKNGTTKKLGQLAILPSILNINEPIIFGLPILYN
                     PVLFVPFVLGNFVNAAIAYVALDMGSVAIPSTYSLLTYLPAPLTAYLLTQDIKAVILI
                     FGLIAIDMLIWAPFLRIHEKTFAIRKSTDGRKVTT"
     gene            complement(4630..4935)
                     /locus_tag="PLL88_07940"
     CDS             complement(4630..4935)
                     /locus_tag="PLL88_07940"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01533.1"
                     /translation="MYQVLLVCQGGMSSSFIVKKIRAAFKNHNEEIEIHAHAGMELVD
                     YIDAVDTVLVAPNILYMWDDVESICKNHGIEPITIPLEHYGNMDGEAIRKIIIGSKE"
     gene            complement(5351..6661)
                     /gene="thrC"
                     /locus_tag="PLL88_07945"
     CDS             complement(5351..6661)
                     /gene="thrC"
                     /locus_tag="PLL88_07945"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOJ01534.1"
                     /translation="MKENSWLECIRGCGVKHAIDEMIYQCERCGGLLDVVHDVEALKR
                     RPALEWKELFAQRAGLRTGIFASGVWAKKEWVCPQVPEHAIVSLNEGNTPLIPTPRLG
                     RQIGIPELWVKQCGNSHSGSFKDLGMTVLVSVVNAMIKQGRAIKAIACASTGDTSAAL
                     AAYAAAAGIPAIILLPHAKVSPEQLIQPLANGARVLSLDTDFDGCMRVVQELTQDPAI
                     YLANSMNSLRMEGQKTVGIEIVQQFGWQAPDWIVLPAGNLGNVSALGKGLLLMLELGL
                     IDRLPRIACAQVQNANPFYLAYRSGFKNYHPVVAHASLASAIQIGAPVSYERAVKVMQ
                     RFAGVVEQVGEEELAELCALADRSGLYCCPQTGVALDAARSLATQGIIKPNERVVVIS
                     TANGLKFSQMKTAYHAGRSDVRALKYRNMPVEIEASVEAVMKNL"
     gene            complement(6648..7781)
                     /locus_tag="PLL88_07950"
     CDS             complement(6648..7781)
                     /locus_tag="PLL88_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01535.1"
                     /translation="MKCYGRAALCDFLGERIIYRGLNVMDERLPGLEVIRAMLGLTNG
                     ALPRKTSLEYAQIVVHLLNEARRLDGKKEALQRVVYIGDTLMNDGMAFRNICQMGGWQ
                     GAALITSEDQKPTRLEAQIDERAMVFQNNHWLNLRIFATLLEVKGMAIDEGTAVLIDV
                     DKTALGARGRNDASIDQARLSAAKATLHEILDHNFNQDEFQTAYNLLNRPVFHPFTAD
                     NQDYLVYICMILSCELLELGEIQAAAQTNKLTTFDEFLEQVERRSAALPDRVRAVHRE
                     VYALVKTGDSTPFKQFRQAEYLATVAAMGQLAEDASIEEMLASEILVTREVQEFALLC
                     ADSGALVFGLSDKPDEATKLNSPSSRPLHQVQTHVVGGMNERK"
     gene            complement(7778..8839)
                     /gene="asd"
                     /locus_tag="PLL88_07955"
     CDS             complement(7778..8839)
                     /gene="asd"
                     /locus_tag="PLL88_07955"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOJ01536.1"
                     /translation="MDQRKNKIPVAVLGATGAVGQRFVQLLAEHPWFQITALAASERS
                     SHKRYADACGWHLHDPMPVAVRDMPVVPVEPDMDAHIVFSALPVETARQVEPQFARAG
                     YAVCSNTSAFRLDSNVPVIIPEVNADHLALVKNQKDAYGWEGLLVTSPNCTTTGFAIP
                     LKPIDMAFGLKKVIAVSMQAVSGAGYPGLSYLDIQDNVIPGIPGEEEKMERESRALLG
                     RMEGKQRVDADFIISAHSNRVPVSDGHTICLSLELRSKASIAEIRKLLSDFRSPIFQP
                     HLPSMPRFPIVVREEPDRPQPHKDRDAGEGMQVSVGQIRPCPVLDVRMVAVVHNTLRG
                     AAKGAILNAELLVAEGLIL"
     gene            complement(8820..10277)
                     /locus_tag="PLL88_07960"
     CDS             complement(8820..10277)
                     /locus_tag="PLL88_07960"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOJ01537.1"
                     /translation="MVAKKERFASQHGRTLVMKFGGTSVGSSHAIRQAADIVLGETAR
                     WENQVVVISAMSGVTDALIACAKAAVIGRTDQYEDIITDLYEKHTGVIDELLVHDEKR
                     KQLCIEIDELFETLQTLCRGIHIMGELTPRGMDAVVSLGERMSARIFSVLLQQRGLRS
                     IPVDATRLIVTDGAFQHAMPVMDTTREKARMLLIPLLNENRLPVVTGFIAATQEGITS
                     TLGRGGSDYTAAIMGDCLDADEVWTWTDVDGVMTADPRIVPDALVIPHVSYSEISEMA
                     YFGAKVLHPKTIRPVVERDIPLWVKNTFNPAGSGTRIDGMHRGGGGHITAVTVIRGLS
                     LITVEGHGMLGVPGIAARTFAAVAKHDASVLMISQSSSEQSICFVIPSEAEAQVIEDI
                     EEEMELELTRHDIDRVWAQPNVVIVTAIGAGMRQTPGVSARLFGALGKESINVIAIAQ
                     GSSEYSISVVVNAADADRAVRKIHEEAIVNGSKKE"
     gene            10578..11483
                     /locus_tag="PLL88_07965"
     CDS             10578..11483
                     /locus_tag="PLL88_07965"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ01538.1"
                     /translation="MKKSDHSKTRAICQALLVTMLWSTSWILIKLTISQIPPLLFAGL
                     RYSLASLFLLPVLLKSRHQIKEIKKDDWLLLISLGIVFYTCTQGGQFITLKYLATTTF
                     SLILNFTTIIIALLGIIFLKENPTKIQWIGIIVLLSGVLLYFSRRTLGGEGWMGYAFA
                     GFTVVSNAVASVLGRRINRSGHIAASLTTGISMSIGALLLLTLGLLIEDLPPLNLTHW
                     IIIIGLASINTAFAFTLWNKSLQVLSAVESGILNNSMLVQIALLSWIFLGDTLSVRDW
                     ISLLIATFGMVLINMKMTSRSTQED"
     gene            complement(11513..>11842)
                     /locus_tag="PLL88_07970"
     CDS             complement(11513..>11842)
                     /locus_tag="PLL88_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01539.1"
                     /translation="ATIISSIAFIFSVFSLFNRPEKIPSGIFLAGGIIWLTVIAGYVV
                     WTNIRAHQQNSRETILRRANLKKAITVSKFALPHRSAMGQIIFVFRNARFAKAFKNLN
                     KGQILKK"
BASE COUNT         3000 a         3189 c         2678 g         2975 t
ORIGIN      
        1 gccgtctggg cagaaggctg gtaccctcaa gtgctcgcgc cagatgcgac caccggtgaa
       61 gccatgaaca ccaacaccga gatcaaaatc ctggtcgatc cggctgccaa caaggtcacc
      121 ctgcgtgtgc cacgctccgt ctttggagat tcctctcctg acagttgggc ttacgccgct
      181 gtggtattaa gccaggaggg gtacccatcc accggcgtat ggcgggtgag ggatgtgagc
      241 aaaaatgcag aacaatggcg actaggaggc gcgcccggcg gcaacaacca tacccgtgtg
      301 gtggatatgg tgtgggcgca ggggaatacg cctgatcagg cagccctctt gagcaacttc
      361 actcccaacg acaagccaca ggcagaactg agtttggagg actttgcatt gattcccatg
      421 ttccggcttc aggactaagg aaaagagagg actgaaaagg agccctacac cagcagcatt
      481 tcccgttgcg ggggtagctc tttcatctgg cagtgaaaga tgcaggcgct gacaggaatc
      541 tgcattgtat gattcacaat accgaaatcc tcaccgctta aaaggtctcg ataggttcca
      601 tccggaaaag cggtgaggat ttcgcctttt ttaccctccg tgttgaagac acctgccagg
      661 ctggttttct cagtgaacca aaccgcctgc accgccggtt cggaggtcag ccaacacagc
      721 tcgccttcct tttgagccgc atctttcttc aacgcagcca ggtttcggat gaaatcggtc
      781 aattcataat tgctccaggg aatgacgtct ttctcaaatt ggtccggttt gcgcgcagca
      841 gccgcttcct ccccggcata aatcaaccaa gcacccttgt tgaacgcctg gaacgccgtc
      901 caggcaagag cttgctcgcg agaaggggcg atagccatca cgcgctccgt atcatgattc
      961 tccacatagc gcaacttgac ataatcttgc gggtagatgg cgtcttgcag caccagcagg
     1021 tccagataat agcgcgtgtc cagagttcct ttgaccgccc tctcccaagg acggaagacg
     1081 tcgtagtcgt aagtgaggtc aaacactttg tacagttcgc tgtctgactg ggcgaacaga
     1141 cctttgctgc gcctctccgc cacccaaccg gtatgcaccg attctgccag ccagatgata
     1201 tctggattga tagctttggc tgctgcatcc gctttcaacc agaattccat cggcaccaat
     1261 gaagccacat cgcaacggaa tccatccaat ccaaaagaag tccacttttg aatagccttt
     1321 acgagatgat catgcaattc cgtatgggcg tgatccagct ccacaatatc cgtccaatcg
     1381 ggcacggagg tggtgggatt gccctgccgg tcgaggcgga accattcttt atgctctctc
     1441 gccaaaaccg aatcagggga agtatggttg taaaccacat cgatcatcac tttcaaacca
     1501 agttcatgcg cagctttgat gagattctgg aaatcctttt ccgtcccata ttcagggttg
     1561 acagccatga aatccgcaat ggcatacgaa ctgcccagtg aacccttacg gttcactttt
     1621 ccgatgggat gaatgggcat gagccagata taatccacac ccagcgcttt gatgcgcagc
     1681 aggtcattga ccactgcctg aaaattgccc tgttggctgt ggttacgaac gtagatctca
     1741 tagatcacta cattgcgaag ccctgttcta tttttcatat gcaccttttc ttaagtagat
     1801 tatagaatga tttttatggt cgcattttta agcgagcagg aaaaaagtag cataacacac
     1861 tctgatataa tatcaatgag cggttgttca ttgaaaatga tgcagaaatt atcctggcga
     1921 tatgtgattg aggatttaat tattgcgtga tacatttata gtgacagagg aagaacaatg
     1981 tctgaagaat caagatccat ccaatttgct actatgacca ttttgcacgc aggaaatgcg
     2041 cgcaaatata tttcagacgc ctacaaagaa ctcagtgaat ttcacttcga agccgccaga
     2101 aaaaagatcg agcaagcgcg cgaagaagcc ttatttgctc accgtgaaca atccaagatc
     2161 atccatgcgg aatccgatgg ggaatctctg gaattctcct tgttgttgac gcacgcacag
     2221 gataccctga tgaccgcagt ttccgaaatg cacattgcga agaatcttat caatatcgta
     2281 gaaaagatcc aggaagatca ccaaaagcac gcataaacta ttgcatgcag aaatggttca
     2341 cctgattttt ctatttcttc cccggttggt tgatgttttg gttgaacagc ccatatagat
     2401 aggcgtcggc gaaataatcg aaaccaccct cgatgaagct gctctccttg aatttgtttt
     2461 ccttgtccac aatcgcttta tccgacataa ggtatccaaa gaataacccc agcacagccc
     2521 ggtacagcag aggaacattg acgtctttca atttttcgtc cttcgcccgc acctcttcaa
     2581 tagtttttga aacggtcgta aaagtcttct caaacaatgc cgggatgtgc tgaccgttga
     2641 attcaaggac ttcgatgaga tgcagtcgga tcaattctgg tcttttatcc cactctctca
     2701 ataacttttc cgaagcgtct cgaatatatt cttcaacggt tttcccactg gcgtttctta
     2761 acacttcggg aatgcgcaac caggggtgat aggtatccag aaccgcttcg aataacgtta
     2821 ttttactgtc gaagtgatta tacagagtcc ctgcaaccag accaagctta tccgtgatct
     2881 gacgggtggt tgttgcatgg aatccattca aaatgaaaag atcacaggca gcatcaatca
     2941 attccttccg agtttgttca ccccgcaatg aataatcttg gtttgtgtta tccatgataa
     3001 aagtactcta ccaaaaaaat gaagtccttt tttaacaaga acagaggttc cctttgataa
     3061 ttatacaaat aaactggatg aatggagctt cataaagcga tgaattatgt attttctttc
     3121 tgccccagca taaaaatcac atcccatatg acgggttact ctttcataac aatccacaga
     3181 ttttttatca attttcacca tacgtcctga tgccatgtct acttcaccag caatcctctc
     3241 agagcgtgcg ttcgttgttg tatttcagct gttccctttc aagacctttc cctgatttct
     3301 cctcgaagcg ctccatgatc agagcagatg ctaggttgta accttcctcc catccgtgga
     3361 tttccggatt gcaaaagtct tttcgtggat cctcaaaaac ggagcccaga tgagcatatc
     3421 gatggcgatc aatccgaaaa tcaggatcac tgctttgata tcctgggtca atagatatgc
     3481 cgttaaggga gccggtagat aagtcaacag ggaatatgtc gatggtattg ctaccgaccc
     3541 catatccagg gcaacgtatg cgattgccgc attcacgaaa tttccgagaa cgaaaggaac
     3601 gaacaaaacc ggattatata gtatgggtaa tccgaaaatg atgggttcat tgatattcag
     3661 gatgcttgga agaatcgcca attgacccaa tttttttgtg gtgccatttt ttgaaaaaag
     3721 catcaaaata ttcaatccaa gcgtggcgcc cgcgccacca atattcatca agaacaacgt
     3781 tcccatattc acgacgaaga atccgtttgc tacggtggga gttaatcctg cagccagcag
     3841 ttgaacattg tttgcgagag ccgcataata gacaggaccg accaggctca tgatcgacgc
     3901 ggggtggatg ccaaaataga agagcagggg aggtagtgag acgaccaaga tccagccccc
     3961 gaagctgccc aagataccca tcaatggcgt tagaagattg ttgaaaatag tatgcaaatt
     4021 gatgttcaac acatctgcaa ctacccaggc agaaaggaca ataaaggtac cggcgatcag
     4081 attctcaaac cacactttgg caatatccgg aaccccttcc gaattcatga tccaaccatg
     4141 cctcttgaag aaagcagaga cctccccagc ccagatacct gccaccatcg agacaaaaac
     4201 ggcttgcatg ccaaacctgg caaatggcgt tgtgaaaacc gaggtgtagt tttcatccac
     4261 tgtgatggaa ggtttcatga aaataaagaa taccgcgatc gccagaaatc ccacagcctg
     4321 ttgatctgtc ttgttcagtt ttgcattagc cgccgcaatg ccataggtaa agacaagtcc
     4381 ggtaacacca aaaaccagat ctttcactgc attgaatttg gtaagaaatg cagacgcgct
     4441 tactgccggt aaccaaccaa gcacgatcga aagcaccgac agcattgctc caaccatgag
     4501 aatacttacc ccagattgaa aaccagcgga gatactttta atgattcgaa aattggcaaa
     4561 tcgactcatt ggttgaacaa atgctctttc aaaccatttc ttctcctgtg ccattttccc
     4621 tccgactttc tattcttttg aaccaataat tatttttctg atggcttcac cgtccatgtt
     4681 tccgtaatgc tccagcggaa tcgtgatcgg ttcgattcca tggtttttac aaatcgattc
     4741 cacgtcatcc cacatataga ggatattggg ggcgaccagg acggtatcga ctgcatcaat
     4801 ataatccacc aattccattc ccgcatgggc gtggatctcg atctcttcat tatgattttt
     4861 aaacgcagcc ctgatcttct ttacgataaa agaagaagac atccctccct ggcacacaag
     4921 caacacttga tacattctat ttttacccta atggacgtac acaatctacc acagaataca
     4981 ccattttctc ctttctacgt tttttagcag gaagatcaca atgataaacg agcgcttatt
     5041 catggtaatt gtagtggggc atacccaaaa gtcaagaaga aagcaaacaa atcatcatcc
     5101 gtgctctccg cctcggatac attaaaccgt gcaacgttcc cccttcgcca caaaaacgtg
     5161 aggttaatgc ctaatctttt aaccctgagc ctgcttaaat ttcttaatct ctgaaatacc
     5221 ccaaccgctc tacatgacgc caaaaaaccc gaaatgggaa agttgagcga attgtcgatc
     5281 atcatgctct cctggatggg tttttcaaca cacccatttc atcataagaa aatttttaga
     5341 aatgaaacag ttacagattc ttcattaccg cttccacgct tgcctcgatc tcaacaggca
     5401 tatttcgata tttcaaagca cgcacatccg accgcccagc atgatatgcc gtcttcattt
     5461 gcgagaattt cagtccattg gctgttgaaa tcaccacgac tcgttcgttt ggtttgataa
     5521 taccctgcgt tgccaggctg cgcgcggcat ccagcgccac cccggtctgc ggacaacaat
     5581 acaagccgct gcgatcagcc agcgcacaca gctctgccag ttcctcttcg ccgacctgtt
     5641 cgaccacgcc tgcaaagcgc tgcatgacct taaccgcacg ttcataactg accggagctc
     5701 cgatctgaat ggcgctggcg aggcttgcgt gtgctacaac cggatgataa tttttgaaac
     5761 cgctgcgata tgccagatag aacgggttgg cgttttgcac ctgcgcgcag gcgatgcgcg
     5821 gcaggcggtc gatcagaccc agttccagca tgagcagaag ccccttgccg agcgcgctga
     5881 cattgcccag gttgcccgcc ggcagaacaa tccaatcggg cgcctgccag ccgaactgct
     5941 gcacgatctc gatgcccacg gtcttttgcc cttccatgcg caatgagttc atggaattcg
     6001 ccaggtaaat ggcaggatcc tgtgtcaatt cctgcaccac gcgcatgcat ccgtcgaaat
     6061 cggtatccag cgagagcacg cgggctccgt tagccagcgg ctggatgagt tgttcagggg
     6121 aaaccttagc gtgtggcaac aggatgatag cagggatgcc ggctgccgca gcgtacgctg
     6181 ccagggcagc ggacgtatca ccggtggaag cgcaggcaat cgccttgatc gctcttccct
     6241 gtttgatcat ggcattcacc acagacacca gaaccgtcat gcccagatcc ttgaaggaac
     6301 cgctgtggct gttgccacac tgcttgaccc acagttcggg aatgccgatc tgcctcccca
     6361 agcgtggcgt tggtattagc ggggtgttgc cttcattcaa cgaaacgatg gcatgttctg
     6421 gcacttgcgg acacacccat tcttttttcg cccacacacc gctggcgaat attcccgttc
     6481 gcagccccgc gcgctgcgca aaaagttctt tccactccaa agccggacgg cgttttaacg
     6541 cctccacgtc atgcaccaca tccagcaacc caccgcaacg ctcgcactgg tagatcatct
     6601 catctatcgc gtgcttcacc ccgcacccac ggatacattc cagccagcta ttttctttca
     6661 ttcatccctc ccaccacatg agtttgtacc tgatgcaaag ggcgcgaaga gggtgagttc
     6721 agcttcgttg cctcatccgg cttatcggat agcccaaaaa ccagcgcccc gctatctgca
     6781 cataataacg cgaattcctg cacctcacgg gtgacgagga tctcgcttgc caacatctct
     6841 tcaatcgaag catcttctgc aagctgcccc atggctgcca ccgttgccag atattccgcc
     6901 tggcgaaatt gcttaaaagg tgtagaatca cccgttttca ccagcgcgta aacttctcga
     6961 tgcaccgcgc gcactctgtc tggcaacgct gccgaccgtc tttcgacctg ttcaaggaat
     7021 tcgtcgaagg tcgtaagctt atttgtctga gcggctgctt gtatctcacc cagttccaac
     7081 agctcgcagc ttaagatcat gcagatgtac accagataat cctggttgtc agcggtgaaa
     7141 ggatgaaaaa caggtcgatt gagcagattg tacgcagtct gaaattcatc ctgattgaaa
     7201 ttatggtcta agatttcgtg cagagtggct ttggcggcgg aaaggcgtgc ctggtcaatg
     7261 ctcgcatcgt tacgcccgcg cgcgcccaac gccgttttat cgacgtcgat caagacagcc
     7321 gtaccttcat cgatcgccat ccctttcact tcaagcaatg tcgcaaagat gcgcaagttc
     7381 agccagtgat tattctgaaa taccatggcg cgctcatcga tttgtgcttc cagcctggtt
     7441 ggcttctggt cttcgctggt gatcaatgcc gccccctgcc agccacccat ttggcaaata
     7501 ttgcgaaaag ccatcccgtc attcatcagg gtatccccga tatagacaac ccgttgcaac
     7561 gcttctttct tcccatccaa tcgccgcgct tcattcaata gatggaccac gatctgcgcg
     7621 tactccaacg aggttttccg cgggagcgct ccatttgtca gccccaacat agcacggata
     7681 acttccaatc ctggcagtcg ctcatccatc acgttcaaac cgcggtagat gatgcgctca
     7741 cccaaaaaat cacataaagc agcccgtccg tagcatttca taatatcaac ccctccgcca
     7801 ccagcagttc agcgttcaag atagcccctt tggcagctcc gcgcaacgtg ttgtgcacca
     7861 ccgcaaccat gcgcacatcc agaaccgggc aaggtctgat ctgtcctact gatacctgca
     7921 ttccctcgcc ggcatcacga tctttgtgtg gctgcgggcg gtcaggttcc tctcgcacaa
     7981 cgatggggaa acgcggcatg ctgggcagat gcggttggaa gatggggctg cggaaatcgc
     8041 tcaataattt ccgtatctcc gctatactgg ctttggatcg cagctccagg gaaaggcaaa
     8101 tggtatgccc atcagaaacc ggcacacggt tggaatgtgc gctgatgata aagtcggcgt
     8161 ctacgcgttg tttgccctcc atgcgcccca gcaaagcgcg cgattcgcgc tccatctttt
     8221 cttcctcacc agggatgcct ggaatgacat tatcctgaat atccagatag gagagccccg
     8281 gatagcccgc tcccgaaact gcctgcatgc tgaccgcgat cactttcttc aacccaaaag
     8341 ccatgtcaat cggcttcaaa gggatggcaa agccggtcgt ggtgcagttc gggctggtga
     8401 cgagtaaccc ctcccagccg tatgcatcct tttggttttt tacaagagcc agatgatcag
     8461 cgttcacttc gggaatgatg accggaacgt tggaatctaa gcggaaggct gaagtattgg
     8521 aacacactgc atatcctgcc ctggcaaact gcggttctac ctgacgggct gtttctaccg
     8581 gtaaagcgga aaagacgatg tgggcatcca tatccggttc caccggcacc accggcatat
     8641 cgcgtaccgc gacaggcata ggatcgtgca aatgccagcc gcaggcatcg gcatagcgtt
     8701 tgtgactgct gcgctcagaa gctgccaacg ctgtgatctg gaaccaggga tgttccgcta
     8761 aaagctgcac aaagcgttga cctacggcgc cggttgctcc caaaactgcc acgggtattt
     8821 tattctttct ttgatccatt gacgatcgcc tcctcgtgaa ttttacgaac ggctcgatcg
     8881 gcatctgccg cgttcactac cacagaaatg ctatattccg atgaaccctg cgcaatggca
     8941 ataacattga tgctctcttt ccccaatgcg ccaaacaagc gcgccgaaac acccggcgtc
     9001 tggcgcatgc cggctccgat agcggtgaca atgaccacat taggctgtgc ccatacccgg
     9061 tcaatatcat gcctggtcag ttcgagttcc atctcttctt caatatcttc gatcacctgc
     9121 gcttcagctt ccgaagggat gacgaaacag atcgattgct cagatgaaga ctgcgagatc
     9181 atcaacacgc tggcatcatg tttggcgacc gccgcaaacg tgcgggctgc aataccgggc
     9241 accccaagca tgccatgtcc ttctacggtg atcaaactga gccctcgaat gaccgtaacc
     9301 gcggtgatgt gcccaccacc gccgcgatgc attccatcaa tacgcgttcc agagccggca
     9361 gggttgaagg tgtttttcac ccacagaggg atgtccctct ccacaaccgg tcgaatggtt
     9421 ttcggatgca ataccttggc gccaaaatat gccatttccg atatttcgct gtacgacacg
     9481 tgagggatga ccagggcatc tggtactatg cgcgggtcgg cggtcatcac gccatccacg
     9541 tccgtccacg tccacacttc atcggcatcc agacaatcac ccatgatggc tgcggtgtag
     9601 tcgctgcctc ccctgcccag cgtgctggta ataccttctt gcgtggcggc gataaagccg
     9661 gtcaccactg gcaatctatt ctcatttagc aatgggatca gaagcattct ggctttttca
     9721 cgggtagtat ccataacagg catggcatgc tggaacgcac cgtctgtcac aataagccgg
     9781 gtggcatcca caggtatgct gcgcaatcca cgttgttgca gcagcacgct aaaaatacgg
     9841 gcgctcatgc gctcgcccaa agatacaacc gcatccattc cccgcggggt taactccccc
     9901 atgatgtgga ttccccggca caacgtctgc aaggtttcga acaattcgtc aatttcaatg
     9961 cagagttgtt ttcgtttttc atcatgcacc aacagttcat caatcacgcc ggtatgtttc
    10021 tcatagagat cggtgatgat atcttcgtac tgatccgtcc tccctataac cgccgctttg
    10081 gcgcaggcga tcaacgcatc ggtcaccccg ctcattgcag aaattaccac cacctggttt
    10141 tcccagcgtg cagtttcccc cagcacgatg tctgcagcct gccggatggc atggctgctg
    10201 ccaactgaag ttcctccaaa cttcatgacg agagtcctcc cgtgctggct ggcaaatctt
    10261 tcctttttgg ctaccataag cacctctctt gaatgaatca tgacagataa agaaaaacgc
    10321 ctcgttgggt aacgaggcgt ttctttttct gttttgtaga tagagcggat ctactgccgt
    10381 ttgttgccga aaacgcgtcg caaccctgca tcacgattgt gatgagtagt agcggtagca
    10441 gtagttgtgg ttgcgaaata tcggctgtac ataattgggc aatactatac catcaccatt
    10501 tcacaaagtc aagcgattca tagatccttg gttcatagat tttggtttat ggggatcgat
    10561 gagataatac ttttatcatg aaaaaatcgg accactcgaa aacaagagct atctgccagg
    10621 cactgttggt taccatgttg tggtcaacct cctggatctt gatcaagctg accatctcac
    10681 aaattccacc gcttcttttc gcaggattgc gctattcgct cgcttccctt ttcttattgc
    10741 ccgttttgct taaaagccgc caccagatca aagagatcaa aaaagatgac tggttgcttt
    10801 tgatcagtct gggcatcgtt ttctatacat gcacccaagg agggcagttc atcactttga
    10861 aataccttgc aacgaccacc ttcagtttga tccttaattt caccaccatc atcattgctc
    10921 ttctggggat catcttttta aaagaaaatc cgactaagat tcaatggata gggatcatcg
    10981 tacttcttag cggtgttctc ttatatttct cacgccgcac tttggggggt gaagggtgga
    11041 tgggatacgc gtttgcgggt ttcacggtgg tttctaatgc ggtcgcgtct gtgctgggca
    11101 ggcggatcaa tcgttcaggg catattgcag ccagcctcac gaccggcatc agcatgagca
    11161 ttggcgcgct gttgctgctc actctcggat tgcttataga agatttgcct cctctcaacc
    11221 ttacacattg gatcattatc attggactgg cttccatcaa cacggctttc gccttcaccc
    11281 tctggaacaa atcgctgcaa gtgctcagcg ctgtggaatc aggtatcctc aacaattcca
    11341 tgctggtaca aatcgctttg ctttcctgga tttttttggg ggatactctt tctgttcggg
    11401 attggatcag cttgctgatc gccactttcg ggatggtact gataaacatg aaaatgacct
    11461 cacgatcaac gcaagaggat taatatacac accagataag cttaaaatat ctctactttt
    11521 taagtatttg acccttattc aaatttttaa atgcttttgc gaaccgagca ttacgaaaca
    11581 caaaaataat ttgccccata gcagaccggt gaggaagagc aaatttgctc accgtgattg
    11641 cttttttcaa gttcgctcga cgcaggattg tctctcgtga attctgctga tgagcgcgaa
    11701 tattcgtcca aaccacatat cctgcaatta cagttagcca aataattcct cccgccagaa
    11761 agatacctga tggaattttt tctggtctat tgaaaagaga aaaaacagag aaaataaaag
    11821 caatgctgct aataatggtt gc
//
LOCUS       DAOSDS010000064        11784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2100_length_11784_cov_4.690340, whole genome shotgun sequence.
ACCESSION   DAOSDS010000064 DAOSDS010000000
VERSION     DAOSDS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2100_length_11784_cov_4.690340"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1489)
                     /locus_tag="PLL88_07975"
     CDS             complement(260..1489)
                     /locus_tag="PLL88_07975"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOJ01540.1"
                     /translation="MNKIMKWLETSFAPTMRKINSNVWIVTVKDSILQILPFILLGSL
                     FCIIAIWVPAFWSPFGWTMGMLSLFVSFLIPFNLMEKKGLRKQRLIGGMTGLVLFLLV
                     VSPQVIKDGTIGFGHESLGAGGMFIAIFCGLFTGAIMNAFGKFSFFKEDSVMPDFVRA
                     WFDAMLPIGIVICVGWLVVEIMSLDLYTALLSVFKPLAGFIESWYGFILLMFLYTLLY
                     SMGISTWVLSPVAKPVTLAAITANIALVASGAAITAQSLNLVTAETIYSVYLWVGGTG
                     CTLPLVIMMLRSKAKSTKALGTASLVPGIFNINEPVVFGAIAWNPILMVPMWLQGIIL
                     PALVWIFTKVIPFAPIPATLFDAWYMPFPISTWLTTRSVNAVILMVAVVVVSTLLWYP
                     FFKIYEKQQLELEAKNS"
     gene            complement(1506..1817)
                     /locus_tag="PLL88_07980"
     CDS             complement(1506..1817)
                     /locus_tag="PLL88_07980"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ01541.1"
                     /translation="MKEIRILLICGSGASSGFLAANIRKAAKNRGLDVVVNARSEAEI
                     ENYVNDIDCLLVGPHLAYLLEDVKRIIQDRPVKVALIDKENYARLDGEKTLDQVLTLF
                     E"
     gene            complement(2093..2995)
                     /locus_tag="PLL88_07985"
     CDS             complement(2093..2995)
                     /locus_tag="PLL88_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOJ01542.1"
                     /translation="MNSDQLEQFRTIAVHGNITKAAEVLYLSQPALSKTLKNLETELG
                     CKLFYRVGRKLNITKEGKRLLEYANTVISTLDIAKQEFLSLEKGRKIRLYSTGYYLPE
                     LLKGYYEKNISDLELHVVPDDAIPAMLIDKHAEAVIADDYYLRHYGTKDLEKILLFKE
                     QLLFCVPKGHRLCFRDWLPLREIQGEPLLYIDMHADMDSWITEVLKLNNCKLNIKLRL
                     DTLLFEKMREDLNYPYLESSVTNLNNRDPEYLADRKQIRINAMYTSRYIYLWFYRNNR
                     KNLSSLIEAIKANAQKVADKAEML"
     gene            complement(3471..4274)
                     /locus_tag="PLL88_07990"
     CDS             complement(3471..4274)
                     /locus_tag="PLL88_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOJ01543.1"
                     /translation="MKLLFQSDDYGITEGVACGTLKGIRDGLIRNTGLFVNMPSSEFA
                     AEQIKYYPQCCFGIDINLVAGRPISPLDQVPSLVKPSGEFYTSGEIVAKSDISLMDLK
                     FDKMENDPYPLDETLIEADNQVKRFMELVGKKPGYIQGHSLMTPNINKALEMMAEKYE
                     IPYTMNFLKEHNFHWVTINWYIRPFPVEKQLQIDVEEGLMKVIPEVLDHEYSFLVSHV
                     GFVDEDLFRCSNYTIIRAKDLFMACSPRLKAFVETHKVELITYDDLKKG"
     gene            complement(4278..5201)
                     /locus_tag="PLL88_07995"
     CDS             complement(4278..5201)
                     /locus_tag="PLL88_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01544.1"
                     /translation="MKDLLMLFGVTLAKRYTNRQKRFFRSQVEPFFKKLGYTVEFQAV
                     KKKLIHVSNILIGDVHKAQYIVLCPYDTPSKSLLPYKYYPYNMSENLRQENLELFLRS
                     LIYVGSCFLAYFTFKQFAFLPTFWKVFSVILSAGLIFFCYRLIVGIPNPVNFNRNSAS
                     VALLAALAERSRKNTDIAYVLLDNTVSSNTGWRLFADDESLKNKMIIYIDCVSYGDQL
                     VCAHNQTTSLEAKKLVGFLTGVDVMDHVFSEEQLKGTNLQFIPKMLHVCTGTIENHDF
                     LVRNTRSKKDFKIDVPRLEILCNGLQNYLGG"
     gene            complement(5267..6088)
                     /locus_tag="PLL88_08000"
     CDS             complement(5267..6088)
                     /locus_tag="PLL88_08000"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOJ01545.1"
                     /translation="MNGKKTENTAEKKILKSMFLRSHLAFIGFNMVKMQANGFTLTMA
                     PAIEEIYKDDPEGKKAAYLRHQSFINTHAVPFSLIAGLTYALEKQNKETSDFDPEVIN
                     SVKASLMGPTAGMFDSLFFNGVRVIAGGIAIGLGSTGNILGALLFILLYGVTQSVAKY
                     YLLNAGYKYGTSFIDQVFGSGLINALTKAAAILGLMMVGALTASMVYVPLNWTLSISG
                     ASLAVQDVFNNIFPGLLSIIYIFVLVRLIKKSIRPTVLIFAILAMSLLGALVGIF"
     gene            complement(6085..6879)
                     /locus_tag="PLL88_08005"
     CDS             complement(6085..6879)
                     /locus_tag="PLL88_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOJ01546.1"
                     /translation="MVTAALKACLVYWIVYTLELNFLSWQTLTRPIVLGPLVGLALGD
                     VKTGIILGASLESIFMGVSAVGGSLPSDWATATVIVVSYAILTGQNAEAGLALAIPIG
                     TIVMSLGAVWSPVTAGLAPYWEKLAGEGKIKKLFTLTSLQFTVITLYTSILMFLAIAF
                     GIEGLGALMAKLPVWVLSGLGASASLMIGVGIAILTSMIWSPKVGVFFFLGFVLAKSL
                     GLSTLAIAIIGIVIAVTVFMQDKDLIGLKNSLGKKSQSSKEGGFFA"
     gene            complement(6891..7388)
                     /locus_tag="PLL88_08010"
     CDS             complement(6891..7388)
                     /locus_tag="PLL88_08010"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ01547.1"
                     /translation="MIKLVRIDERLIHGQVAIRWSKFLGVDRILVLNNEAAKNDLIKK
                     SLMMAAPQDVKVAIETMENGIEMLNDPRAASLSILVLVKTPHDVLTLMNSVKGIEAVN
                     VGNYGRVAPKEGTAVRKTFRLNLYLYDSELMVIKKILETGVKCDYQVTPDDKPEPLKK
                     IIENT"
     gene            complement(7385..7795)
                     /locus_tag="PLL88_08015"
     CDS             complement(7385..7795)
                     /locus_tag="PLL88_08015"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ01548.1"
                     /translation="MAASHGTFASGIKSSLDVLLGKSENVTVIDAYLGQESVEDQLDA
                     YFRSIPETEQVIMLSDIYGGSVNQKMYLRLARPNTFLITGINLALVLQLLMETGPLSL
                     ERLRTIVEEGQRALMLVEFQSAGVEDEDFFGESK"
     gene            8105..8899
                     /locus_tag="PLL88_08020"
     CDS             8105..8899
                     /locus_tag="PLL88_08020"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOJ01549.1"
                     /translation="MKKLIVVGDDFGFSEAYSIGAVKAYKEGIVTVLSLMNNMDTAQF
                     AVNLWKKECPDAPLAQHTNFVQGKPVSNNVNSMVDENGYFYKSTMWKSDHPMGEKGKG
                     NVVVTYEDAKRETIAQLEHHKELTGSYPTHFEPHSIGSANTQRAFKEVMEEYKIHAGC
                     QEREDEYLYYGHELLMTNEAYLKALHEGITAEQFMNDICGILSSPYEYNILHFHPGYL
                     DAYVFDNSTLTIPRVRDLQLVTDPRVRKWIDDNNIELVDFSVLYKK"
     regulatory      9183..9302
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            9391..10581
                     /gene="metK"
                     /locus_tag="PLL88_08025"
     CDS             9391..10581
                     /gene="metK"
                     /locus_tag="PLL88_08025"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOJ01550.1"
                     /translation="MESPKYLFTSESVTEGHPDKLCDQISDAVVDACLEQDSNSRVAC
                     ETAAKTGFVMLLGEVSTKAVINYDELARDVIKDIGYDHTDTGFDYNTCGVLVAICKQS
                     PDIDMGVSKAKEAKEGKMDDIEAIGAGDQGMMFGFACNETEVLMPMPIYLAHKLTRRL
                     SEVRKDGTIDWLRPDGKSQVTVEYRYGKPARIDTVVISTQHAPEVSHAEIEKEVIEKV
                     IKPTVPAGMLDANTIYHINPTGRFVTGGPKGDSGVTGRKIIVDTYGGMGRHGGGCFSG
                     KDATKVDRSAAYAARWAAKNVVAAGLADRCEIQVAYAIGVARPVSINVETFGTGKITN
                     EKIAELIYDHFDLRPGAIIRDLDLRKPNFRKNTNYGHFGRDDLKLTWEQTNKADALRK
                     AAGL"
     gene            complement(10638..>11784)
                     /locus_tag="PLL88_08030"
     CDS             complement(10638..>11784)
                     /locus_tag="PLL88_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOJ01551.1"
                     /translation="PNSEAALVTLREGMESVAFQKLDSPLKIVLGENVAGKPVAVDLR
                     DMPHLLIAGTTGSGKSVCINAIISCLLMQRTPEEMRMVMIDPKRVELTGYNRIPHLLT
                     PVVVDPEKVIGTLRWISREMDGRYHRFAEVGARNIQDYNRTQPVKIPYIVIIIDELQY
                     LMSIAPDETESSFARLAQMARATGIHLIIATQRPSVNVITGVIKANFPARISFMVVSG
                     VDSRVILDQVGAERLLGKGDMLFQAPNEPSPKRLQGVFLSEEEIQRLVHFWVRQAEEL
                     KAATPGEDIAKSMEKVFPVGETVTEVPLFDSPLEIDEDPLLKRAIAFVRSEERASISM
                     LQRKFSIGYTRAARLVDRMEELEIIGKPDSGSGVRPVLNFGDEENAE"
BASE COUNT         3369 a         2884 c         2569 g         2962 t
ORIGIN      
        1 acgctttttg ataatcatcc tctgctattg ccccaagagc cttgtgggta aataagcggg
       61 catcacccgc atggatgatg atttccatcg ctgcattcac tatttcttct ttttcaccca
      121 ttttgatcaa ctccatacaa tttcatcatt aacgactttt cacgcgaaca ttctctcttg
      181 aaaaaagaac aggctctatt cttaaggaag gaatcggcag gaatatagag cctgtctcac
      241 ttctcaacgc atcacaagat taactgtttt tagcttccaa ttcaagctgt tgtttctcgt
      301 aaatcttgaa gaacggatac caaagaaggg tagaaacaac cacaacagca accatcagga
      361 taaccgcatt gacactcctc gtggtaagcc atgtggaaat tgggaaaggc atataccagg
      421 catcaaacaa cgtggcggga ataggagcga acgggataac cttcgtgaag atccacacca
      481 gcgctggcag aatgatacct tgaagccaca tgggcaccat caagatcgga ttccaggcaa
      541 tcgcgccgaa caccacaggc tcgttaatat taaaaatgcc tggaacaagg ctggctgttc
      601 caagagcttt ggtcgacttc gctttcgacc gcagcatcat gatcacgagg ggaagagtgc
      661 agccggtacc accgacccac agatagactg aatagatcgt ctcagcggtc accagattca
      721 aagactgcgc agtgatggcg gctcctgaag cgaccaacgc gatgttcgcg gtgatggctg
      781 ccagcgtcac aggtttggct accggcgaca gaacccaggt ggagatgccc attgaataca
      841 ataaagtata gaggaacatc agcagtatga acccatacca ggattcaatg aaccctgcca
      901 aaggtttgaa aacagaaagg agcgcagtgt acagatccaa cgacatgatc tcaaccacca
      961 gccatcccac acagataacg atcccgatag gaagcatggc atcgaaccag gcacgcacaa
     1021 agtcaggcat cacagaatct tctttgaaga atgagaactt tccaaaggca ttcatgatag
     1081 cgccggtaaa aagaccgcag aagatggcga tgaacattcc gcctgccccc aaagattcgt
     1141 gtccaaaacc gatcgtccca tctttgatga cctgagggga gacaaccagc aagaacaaaa
     1201 ccagcccggt catgccgcct atcaagcgct gtttccttaa tccctttttc tccatcagat
     1261 tgaatgggat caggaatgaa acaaacaaag aaagcattcc catcgtccaa ccgaacggcg
     1321 accagaaagc aggaacccag atcgcgatga tacaaaataa cgaccctaag agaataaaag
     1381 gcagtatctg taaaatagaa tctttgacgg tgacgatcca aacattgctg tttattttcc
     1441 gcatggttgg cgcaaagctg gtttccaacc atttcatgat cttattcatt atttttcctc
     1501 caaatttatt cgaataaagt taaaacctga tccagggttt tttctccatc caatcttgca
     1561 tagttttcct tgtcgatcaa tgcaaccttg accggtctat cctggatgat gcgttttaca
     1621 tcttccagca aatatgcaag gtgaggtcca accagcaggc aatcgatatc attgacatag
     1681 ttctcaatct ccgcttcact ccttgcattg acgaccacat ccaatcctcg gttcttggct
     1741 gcttttcgaa tattcgctgc cagaaaacca ctagatgccc cagatccaca gattaacagt
     1801 attcttattt ctttcaactt tgtcctcttt accttatttt ctttacgagt aactatacac
     1861 ggctttcaaa cccagatgaa tatctacctc ctttctttac aagagagcga aatgctgaaa
     1921 tcacagtaaa gattactagt ctaagtatag ggagtgaaaa atgagatgtt aaataccaac
     1981 aatctatatc acccataact cagggttata ggttctactt ccttaaaaat gggggctgca
     2041 ggatattttc ttttctacaa gattgttagg acagaaaaac agacgaaacg ggttacaaca
     2101 tttcagcctt atcggctact ttctgcgcat tcgctttaat cgcctcaatg agactggaaa
     2161 gatttttgcg attgttccga taaaaccata aatagatata cctgctcgta tacatggcat
     2221 tgattctgat ctgttttcga tccgccaggt attccgggtc tctattattc agattggtga
     2281 ctgagctttc caaatatgga taattcagat cttcgcgcat tttttcaaat aacagggtat
     2341 cgagcctcaa tttgatattc aatttgcagt tgttcaactt caacacttcc gtgatccagc
     2401 tatccatatc tgcgtgcatg tcgatataca aaagcggctc tccctgaatc tccctcaaag
     2461 ggagccaatc tcgaaagcat aagcgatggc cctttggaac acaaaaaaga agctgctctt
     2521 tgaacaatag aattttttcc agatcctttg tgccataatg ccgcagataa taatcatctg
     2581 cgatcactgc ttcggcatgt ttatcaatca acatagccgg aatagcgtca tcaggcacaa
     2641 catgcagttc cagatcggaa atattctttt cgtaatatcc tttgagcaat tcgggtaaat
     2701 agtagcctgt actgtataat ctgatctttc tgcccttttc taagctcaga aattcttgtt
     2761 tggcgatatc caatgttgaa atgaccgtgt tggcgtattc caaaagcctc tttccctctt
     2821 tggtaatatt caattttcgc ccgacgcgat agaagagttt acaaccaagc tctgtttcca
     2881 gattcttcaa cgttttgctt aaagcaggtt gcgatagata gagcacctcg gctgctttgg
     2941 tgatgttccc atgcactgca atggttctga attgctccaa ctgatcacta ttcattgtca
     3001 aaaacccaca tgctcataaa aaatgagact taacgacggc tgtgttcaag taacggtaat
     3061 ttaaatatta gcacacatca tttctttgag ggggaatatg attattctct tttatctccg
     3121 gcgcacttat gtacactacc accgttcagc actgcacaca gaaattgatc aaatcgattg
     3181 cgtttttaat gcgtttgccc catcttgatt gatggggcta tttctttaag gaaaaaactt
     3241 gatgaattca ggatatgccc gctttcaatt aatcttatac ctggttccac aatctctatt
     3301 gaagaagtca ccacctaccc tgggatcata gacctggcga ccttccactt aggtggcgat
     3361 ggctgcgtca cacccgagaa aatcaacgat ccgcccgtct tcgacgacaa gatagccatc
     3421 acggacgccc tgtccgaaac atagcgagct acattttctg aacatcaaac tcaccccttt
     3481 tttagatcat cgtaggtgat cagttcaact ttatgggttt caacaaaagc tttcagtctg
     3541 ggagaacacg ccatgaagag gtccttcgcc cggataattg tgtaatttga acaacggaag
     3601 agatcttcat ccacaaagcc cacatgactg acgaggaaag aatactcatg atccaacacc
     3661 tcaggaatga ccttcatcaa accttcttca acatcgatct ggagctgttt ttcaacggga
     3721 aaaggtcgta tataccaatt aatggtcacc caatgaaaat tgtgttcctt taaaaaattc
     3781 atggtatacg ggatttcata tttttccgcc atcatttcga gcgctttgtt gatattagga
     3841 gtcatcaggg aatgtccctg gatatagcct ggttttttcc cgaccagttc catgaaacgt
     3901 tttacctggt tatccgcttc gatcaacgtt tcgtccaatg gatagggatc attttccatc
     3961 ttgtcaaact tcaaatccat aagagatata tcggatttcg caacgatctc gccggaggta
     4021 taaaattccc cagatggctt cacaaggctt ggaacctgat caagcggaga aattggtctg
     4081 cccgctacaa gattgatatc aatgccaaaa cagcactgcg gataatactt gatctgctct
     4141 gccgcgaact cggaactggg catgttgaca aacaaaccgg tgttgcggat caacccatct
     4201 cgaattcctt tcaaagtgcc gcacgcgacc ccctcggtga tcccgtaatc gtctgactga
     4261 aaaagtaatt tcatgatcta acctcctaaa tagttttgta gtccattaca gagaatctcc
     4321 aatcttggaa cgtcaatctt gaaatccttt ttggagcggg tgttgcgaac caaaaaatca
     4381 tgattctcga tcgttcctgt acatacatgc aacattttgg ggataaactg caggtttgta
     4441 cctttcaatt gttcttctga aaaaacatgg tccatcacat caaccccagt gagaaaacca
     4501 accaatttct tcgcctcaag acttgtcgtc tggttgtgtg cgcaaacgag ttgatctccg
     4561 taagaaacac agtctatata aatgatcatt ttgttcttca ggctttcatc atcagcaaac
     4621 aaccgccagc ctgtattgga actgacggta ttatccagaa gcacataagc gatgtcggta
     4681 tttttccttg agcgttccgc caaagcagcc agcagggcaa cactggctga attcctattg
     4741 aaattcaccg gattgggaat acccacaatc agtcgatagc agaaaaagat caaaccggca
     4801 gagagaatga cactaaagac cttccagaaa gtgggcagga aggcaaattg tttgaaggta
     4861 aaatacgcca agaaacagga accaacgtaa atcaaagagc gcaggaacaa ctccaggttt
     4921 tcctggcgaa gattctccga catattatac ggatagtact tgtaggggag caaagattta
     4981 gagggggtgt cgtatgggca caagacgatg tattgcgctt tgtgaacatc tccgatgagg
     5041 atgttcgaca catggatcag cttttttttg actgcttgaa attctaccgt gtatcccagt
     5101 ttcttaaaga acggttctac ctgtgaacgg aaaaatcttt tttgtcggtt cgtataccgt
     5161 ttcgctaatg tcacaccaaa taacattaat aaatctttca tgttgcgttc tcatacatga
     5221 aaaaggttat ggagtattgc atccataacc tttttctcaa attatcctaa aagattccca
     5281 ccagggcacc cagcaacgac attgccagga tggcaaaaat cagaacggtt gggcgaatac
     5341 tttttttgat caacctcacc aggacgaata tgtagatgat gctcagcaat ccgggaaaaa
     5401 tattgttaaa tacatcttga actgcaagcg aagcaccact gatggataat gtccagttta
     5461 gaggcacata gaccattgaa gcggtcaatg ccccgaccat catcaatccg agaatagcgg
     5521 ccgctttggt taaggcattg atcaatcctg aaccaaacac ctggtcaatg aaggaagttc
     5581 catatttgta acctgcattc aaaagataat attttgcaac ggactgcgtt actccgtata
     5641 acagaatgaa caggagggca cccagaatgt tgcccgtcga gccgagcccg atagcgatac
     5701 ctccagcgat aacgcgcaca ccattgaaga acagagaatc gaacatgccg gcggtgggac
     5761 ccatgaggct ggctttgacg ctgttgatca cttcggggtc aaaatcggag gtttccttat
     5821 tctgtttttc caacgcataa gtgagcccgg caattaatga aaagggcact gcatgcgtat
     5881 tgatgaaact ttgatgacgc aaataggctg cctttttgcc ctccggatca tccttataga
     5941 tctcctcaat agcaggagcc atagtaagag tgaatccatt ggcttgcatc tttaccatat
     6001 tgaatccgat aaaggcaaga tgcgagcgca ggaacatcga tttgagaatt ttcttctcag
     6061 ccgtgttttc ggtctttttg ccgttcatgc gaaaaatcct ccttctttcg acgattgaga
     6121 tttctttccc agcgaattct tcagaccaat aagatcttta tcctgcatga agactgtaac
     6181 tgcaatgacg atgccgatga tcgcaatcgc cagcgttgag agtcccagag atttagccag
     6241 gacaaaaccc aggaagaaga acacaccaac tttgggagac cagatcatgg aagtcaagat
     6301 cgcgatacca acgccgatca tcaatgatgc ggaggctccc agaccactca acacccacac
     6361 gggcagtttt gccatgagcg cgcccaaacc ttctataccg aatgcaatgg caaggaacat
     6421 tagaatagaa gtatatagag taatgaccgt aaattgcaat gaggttagag taaacagttt
     6481 tttgattttt ccttcacctg ccaatttttc ccagtagggc gcaagtccgg ctgtaaccgg
     6541 tgaccaaaca gctccaagag acatgacgat cgttccaatg ggaattgcaa gagccagacc
     6601 cgcttcggca ttttgaccgg tcagtatggc ataggaaacc acgatcacgg tcgctgttgc
     6661 ccaatcagaa ggaagcgaac caccaactgc ggacactccc atgaaaatgg actccagtga
     6721 ggcacccaga atgatgcctg ttttgacatc accgagggca agacccacca gtggacccaa
     6781 gacaataggt cgggtcaggg tctgccaact caaaaagttt aattcaagag tgtaaacaat
     6841 ccagtagacc aagcaagctt ttaacgctgc tgttaccatg tattcctcca ttaagtattt
     6901 tcgattattt ttttcaaagg ttctggtttg tcatctggtg ttacctgata atcacacttc
     6961 acaccagttt ccagaatttt tttgataacc atcagttcgc tatcgtacag ataaagattc
     7021 agacggaatg ttttgcggac cgctgtgcct tctttcggtg caacgcgacc ataattgcca
     7081 acatttaccg cttcgatccc tttcacggaa ttcatcagcg taaggacatc atggggggtt
     7141 ttgaccagca ccaaaatgct gagactggct gcgcgcgggt cattcaacat ttctattcca
     7201 ttttccatcg tttcgatagc tacttttaca tcttgaggag cggccatcat taaagatttt
     7261 ttgatcaggt cgttttttgc tgcctcgtta tttaacacta atattcgatc cacgcctaag
     7321 aactttgacc acctgattgc aacctgtcca tggatcaaac gttcatcgat tcgcaccaac
     7381 tttatcattt gctttctcca aaaaaatctt cgtcctccac gccagcggat tgaaactcaa
     7441 ccagcatcag tgccctttgc ccttcttcca caattgtcct taaccgttcc aggctcagtg
     7501 gtccagtttc catcaacaat tgcaacacca gagccaaatt tattcctgtg ataagaaacg
     7561 tattggggcg ggcaagacgc aggtacattt tctgattgac actgccccca taaatatcag
     7621 agagcatgat gacctgttca gtttctggaa tagatcgaaa atatgcatcg agttgatctt
     7681 cgacgctttc ctgacccagg tatgcatcaa tcaccgttac gttttccgat ttaccaagca
     7741 ggacatcgag agaactttta atgccactgg cgaaagtgcc gtgtgaagcc gctataattt
     7801 tcagaaaaaa tcacctcctt atagaacaat ttccgagccc tacaattcaa ctcattcttt
     7861 cgataagcag aaaatcctcc tttctttcta ttttttaaac gggaaagccg tgttattata
     7921 aatgagcgct tgttcattaa ttataatgaa tatgaataat tagtcaagaa atagaagata
     7981 ttaacgtatg agcaaatgaa tcgattgggg tttcataaaa aatattgaga aaggaggaaa
     8041 gtaccggaaa gaagaaacca agaaccaaat ttaaccattc gctataaaaa acaaaggaca
     8101 cactatgaaa aaactgatcg ttgttggaga tgactttgga ttcagtgaag catatagcat
     8161 tggagctgta aaagcatata aggaaggcat tgtaaccgta ctttcattaa tgaataacat
     8221 ggatacagca cagtttgcgg ttaatctctg gaaaaaagaa tgtccagatg ctcccctagc
     8281 tcaacatacc aattttgtgc agggaaagcc tgtatccaat aatgttaaca gcatggtaga
     8341 tgaaaatggg tatttttata aatctaccat gtggaagtct gatcatccca tgggtgaaaa
     8401 aggcaaaggc aatgtagtcg ttacgtatga agatgcaaaa cgggaaacca ttgcacagct
     8461 tgaacaccac aaagaattga ccggttctta tcccacccac ttcgaaccac attcgattgg
     8521 ttcagcaaac acacagcggg cgttcaagga agtcatggaa gagtataaaa tccatgccgg
     8581 ttgccaggaa agagaagatg agtatctcta ttatgggcat gagttgctga tgacgaacga
     8641 agcatatctg aaagcacttc acgagggtat aactgcggag caattcatga atgatatttg
     8701 tggaattcta tccagcccct atgaatataa catccttcat tttcatcccg gctatctgga
     8761 cgcttatgtg tttgataatt ccacactgac cattccgcgg gtaagggatc tgcaattggt
     8821 tacagacccg cgtgtgagaa aatggattga tgacaacaat atcgaactgg tagatttttc
     8881 agttttatac aaaaagtaaa ggcaaagtta ctccaaaaag catagaaaaa accatgcccc
     8941 gctctacaac cagatcattg aagaaaagga catggttttt tttatgctgg ctgaatcctg
     9001 gttatggaag aaacgacgat tccatagcac ccctaagatt gattgttcgc ttgcagccac
     9061 gtcattcatc caggcaaaaa tatgacaaaa ttcctccaat agatttgccc gtcggtttga
     9121 caactcatgc aggctatgtt ataaaacaga gaattaacaa ctgaatatag ccccaaaagt
     9181 ttctcttatc cagagaggtg gagggatcgg ccctgtgaaa cctcggcaac ccgcagggcg
     9241 cttctagcga agcgctcacg aaggtgccaa ctccggcaga aagacgttct ggaagatgag
     9301 aggaaatgca acaaaaactg cgtttgcccc tcaacctgaa tggggcaatt ttttttaaca
     9361 aaggagcaaa ggaaaacaat ggaatctctc atggaatccc ccaaatatct gttcacctcc
     9421 gaatcggtga ccgaaggaca ccccgacaag ctgtgcgatc agatcagcga cgcagtggtg
     9481 gatgcctgcc tggagcagga ctcgaattcc cgggtggcgt gcgaaacagc cgccaagacc
     9541 ggttttgtaa tgttattggg cgaagtgagc accaaagccg tcatcaacta cgatgaactg
     9601 gcacgcgatg tcatcaaaga tatcggctat gatcacaccg acacaggctt tgattacaac
     9661 acctgcggtg tgctggttgc catctgcaag caaagccccg atatcgacat gggcgtctcg
     9721 aaagcaaagg aagccaagga aggcaaaatg gatgacatcg aagccatcgg cgccggcgac
     9781 cagggcatga tgttcggctt tgcctgcaat gaaaccgaag tgctcatgcc catgcccatc
     9841 tacctggcac acaagctcac ccgccggctt tccgaagtgc gcaaagacgg cacgatcgac
     9901 tggctgcgtc cagatggcaa gagccaggtg accgtggaat accgctacgg taagccagcc
     9961 cgcatcgata cggtcgtcat cagcacgcag cacgctcccg aagtatcgca tgccgagatc
    10021 gaaaaagagg tcattgaaaa agtgatcaaa cccaccgtcc cggcaggcat gctagacgcc
    10081 aacaccatct accacatcaa ccccaccggg cgcttcgtaa ccggcggacc caagggcgat
    10141 tccggtgtga ccggtcgtaa gatcatcgtg gatacctacg gcggcatggg gcgtcatggc
    10201 ggcggctgct tcagcggcaa ggacgccacc aaagtcgacc gctcggctgc atacgccgcg
    10261 cgttgggctg ccaagaacgt ggtggcggct ggtctggctg accgctgcga gatccaggtg
    10321 gcatatgcca tcggtgtagc ccgccccgtc agcatcaatg tggaaacctt cggcaccggc
    10381 aagatcacga acgagaagat cgctgaattg atctacgatc atttcgacct gcgcccaggc
    10441 gccatcatcc gcgacctcga cctgcgcaaa ccgaacttcc gcaagaacac caactacggt
    10501 catttcggac gcgacgacct gaagctgacc tgggaacaga ccaacaaggc agacgccctg
    10561 cgcaaagctg ccggtttgta gatcacgcat tcattaaaca aacgaaaaag agacttggct
    10621 ttcaagtctc tttttgatca ttcggcattt tcttcatctc caaaattcag caccgggcgc
    10681 accccgctgc cggaatccgg tttgccgatg atctccaact cttccatacg gtccaccagg
    10741 cgcgcagcgc gcgtgtagcc gatgctgaat ttgcgttgca gcatggaaat ggaagcgcgc
    10801 tcctcagaac gcacgaaggc gatggcgcgt ttgagcagcg gatcttcatc gatctccagc
    10861 gggctgtcaa agagcggcac ctcggtaact gtctccccaa ccgggaagac tttttccatg
    10921 cttttcgcga tatcttcacc tggggtagca gctttcaatt cctcggcttg cctcacccag
    10981 aaatgaacca ggcgctgtat ctcctcttcc gaaaggaaga caccctgcaa gcgtttggga
    11041 gaaggttcat tgggcgcctg gaagagcata tcgcccttac caagcaagcg ttctgccccc
    11101 acctgatcga gaatgacgcg gctgtcaacg ccggaaacca ccatgaatga aatgcgcgcg
    11161 gggaaattgg ctttgatgac accagtgatg acgttcaccg atggacgctg ggtggcaatg
    11221 ataaggtgaa tgccggtagc gcgcgccatt tgagccagac gcgcaaaact gctctcggtc
    11281 tcatcgggcg cgatggacat gagatactgc aattcatcaa taatgatcac gatataaggt
    11341 atcttgaccg gctgggtgcg gttgtaatcc tggatattgc gcgctcccac ctcagcaaag
    11401 cggtggtagc gcccatccat ctcgcgcgag atccagcgca gcgtgccgat caccttttcc
    11461 ggatcgacca ccaccggcgt gagcaggtgc gggatgcgat tgtagccggt cagttccacg
    11521 cgtttgggat cgatcatcac catgcgcatc tcttcagggg tgcgctgcat gagcaggcaa
    11581 ctgatgatgg cattgatgca caccgattta ccagagccgg ttgtgccggc gatcaggagg
    11641 tggggcatat cgcgcagatc aaccgccacc ggtttgcccg ccacattctc cccaagcacg
    11701 atcttcagcg gagagtccaa tttctgaaac gctacgcttt ccatgccctc gcgcagggta
    11761 accagcgctg cttcgctatt cggc
//
LOCUS       DAOSDS010000065        11686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2122_length_11686_cov_3.990800, whole genome shotgun sequence.
ACCESSION   DAOSDS010000065 DAOSDS010000000
VERSION     DAOSDS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11686
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2122_length_11686_cov_3.990800"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="PLL88_08035"
     CDS             <1..864
                     /locus_tag="PLL88_08035"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ01552.1"
                     /translation="YYGSRSNAVDHLETAMLNGFNRIEVGCNALIADGLTGSDSTDIE
                     INQKYVKAAKIGSAIAAADIVISVSHFKGHQGTGFGGTLKNVGMGSGSRRGKMEMHAA
                     SKPKIREENCVACGKCIQHCSQKAIAYNQNRKAQIDYSKCIGCGQCVASCHYGAAVAL
                     YDENSATVGAKMAEYAYGVLLGKPNFHISLMMDISPECDCWNINDQAIAPNIGMAASF
                     DPVALDRACVDLVNQAPAITTSALFDKTPYQPSQDKFDHIHPDVTWRDTLSHAEEIGL
                     GSQSYELVKIK"
     gene            complement(918..2441)
                     /locus_tag="PLL88_08040"
     CDS             complement(918..2441)
                     /locus_tag="PLL88_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HOJ01553.1"
                     /translation="MKAKNLLIMVGLFLIPIIARTVWYYQGIYIPSKQLQTPDFLSLE
                     IPQPTLSTSVASNAREIKKTGSVVLDLAHANQYQITEIEMLIDELKDRGAEIVTFDEG
                     EDLNSTLKGAAAFISIAPTMDFSGDEIQQIQTFAARGGRLLVIADPTRSSSEYAISRA
                     QSVKVVNTLLEPYHIIFRNDYAYNVYDHEGNFRNVYLEPLDKNELTENVERVVFYASR
                     SISSYETNLLAGDANTLSSLTDTGGDLCMVALAGNNVLAIGDLTFLTSPYYQVADNYQ
                     LIKNIGQFLMSGERQVTFDDFPNLFTKPIGILSGERIVLDQDLLAQINAVKELYAKQD
                     LTVEFLEADDEDYDLIILGLIPPDQTIHKYLDGLAIQFGSQAGNTPTPQPTAAETSAA
                     ATESITAQIPTQATLRAGQIYITGLGEVSQSDFGFFLLKSTSNRTMLLLLADNQGDLV
                     ELLSIVRYGSLKGCYVENAIALCEKNNSGWKPTPTPYVVFEETLELTPTSSPPPITE"
     gene            complement(2438..3301)
                     /locus_tag="PLL88_08045"
     CDS             complement(2438..3301)
                     /locus_tag="PLL88_08045"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOJ01554.1"
                     /translation="MMKKNSQSSKTKNDPRILIFLLPLVLGILIASLVPRPVIGSIHI
                     RDEIDTKMSIRVMKEIEYARSHPEIKAVVLILDSPGGTINDTELIYLELMKLRETKPV
                     VAMVEGLSASGSFYLAMGTDYIISNPSARIGNVGVIGTLPSSPIVLEETISTGPYKLF
                     GNQREGYTRYIELIKESFYQVVKLGRENKLKLSKEEVLRGELYPASEAAKAGLIDEVG
                     PQSSAVAKAAQLAHIAHYRIINISEALAGETSDEKSSFFILDENGASTGYPREPGVYL
                     LYIPDYGSEIP"
     gene            3510..4682
                     /locus_tag="PLL88_08050"
     CDS             3510..4682
                     /locus_tag="PLL88_08050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOJ01555.1"
                     /translation="MKEYKYLIVGGGITAAFAVQGIREKDKDGSIAILTKENYAPYSR
                     PPLSKDLWKGKPVESIWLPVPEENVDILTNHTVMQLTLAAKKVLTQNKQEFGFQTLLL
                     ATGSDSKRLRFSDDDIIYYRNFSDYLKLSDLAEKRNDFAVIGAGFIGSEIAAALAMKR
                     KKVFLLESGAGIGWKIFPQAIVEFLNALYRSKGVEVLDHTLIKDIEKQGERHHVMLKN
                     GKTMPVDGVISGVGVKPNSLLADKAGIYVEDGIKVNSYLETSSPDIFAAGDVAAFHSS
                     ALDKTLRIEHNDNAKKMGYLAGLNMAGGNEEYDYLPMFYSDLFDIGYEAVGLIDVRLD
                     PKVVWKEEFKRGVIYYRKDNVIKGILLWNEWDKVDLAREIIMKQKWIADDDLIKLI"
     gene            complement(4714..5124)
                     /locus_tag="PLL88_08055"
     CDS             complement(4714..5124)
                     /locus_tag="PLL88_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3887 domain-containing protein"
                     /protein_id="HOJ01556.1"
                     /translation="MKRSICISLFIILLLAACQTKTTAVPENFQEISQPMAENLFTSL
                     KNNDFSSFHKDFTEKMLSSISEDQFVSLHDSISQTVGEYQALTYDQSTYEEGYLISYF
                     SVQFSDGSLTLRLVLDSKEPYRISGFWFPDFPSQ"
     gene            complement(5168..6721)
                     /locus_tag="PLL88_08060"
     CDS             complement(5168..6721)
                     /locus_tag="PLL88_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HOJ01557.1"
                     /translation="MESTFVNWLLSFSPILLFLFLMIGKKWGGSKAGFVSWIYTLVIT
                     FTFFGANIPLVGYAHFKAVFLALDVLLIIWMALLFFHISNYAGAIQVLGNALAVFTQN
                     KTLQVLLLAWLFTSFLQGLGGFGVPVAVTAPLLVGLGIDPIDAIIMASIGHGWAVTFG
                     SMGSSFQTMMALSDIAGSTLAPDTAKLLGVMAVISGIWLVQYYAGFKAVLKNLPFVFT
                     IGAILGGVQYLLATNGMWTVATTGASLVALGAAFVLIKIGSSGKNKKAAIQGTTDPFK
                     PSLILSIAIYFILILLSFAIKLIPFINDPLSRFQLTYAFPEIVSNLGWSTAPENGREI
                     TLFTHPGMILLGASIIAFLLYRKKNLLDKEAYKKILSSVKKSGLQSSIAIYFTVGIAV
                     LMSHTGMINILAKGLSGFVGSTLYPAITPFIGALGAFISGSNNNSNILFTKLHMQTAE
                     LLGLNVNLILAAQSAGGAIGSIFAPAKVIVGCSTVGLVGKEGQVLKSLIRHGLFLVAC
                     IAILTLAMS"
     gene            7127..9880
                     /gene="ppdK"
                     /locus_tag="PLL88_08065"
     CDS             7127..9880
                     /gene="ppdK"
                     /locus_tag="PLL88_08065"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOJ01558.1"
                     /translation="MSDKKWVYLFNEVEVAEKYAGSWDGVRGLLGGKGANMAEMIRIG
                     VPVPPFFTVTTEGCNAYIASGGKFPEGMWEQMVVALKAVEKASGKEFGNPKNPLLVSC
                     RSGAKMSMPGMMDTVLNIGLNDETAGGMVALTNNERFVYDAYRRLISMFGSVVLGISD
                     DAFEDVLIDLKKEKKANSDTDLDAAALKELVEKFKKVTRNAKGFDFPQDPLEQIKLAT
                     EAVFKSWNSKRAIDYRRATDIPDDLGTAVNVVTMVFGNMGWNSGTGVAFTRNPSTGER
                     VLYGEYLLNAQGEDVVAGIRNTEKIQTMGEKLPEANKQFLEIAANLEKHYRNMQDVEF
                     TVENGKLWMLQTRDGKRTAAAAVKIAVDMVNEGLISKEEAIQRVTPAQVDTLLHPQFT
                     DETIKQAHKDGHFFADGVDASPGAAVGQIYFDADTAEKMAKEDGQDVIMVRPFTKPDD
                     VHGMLAAKGILTSEGGATSHAAVVARQFGVPCVVGASMIKINMEKRQMEAGGRIVKES
                     EWISIDGGSGEAFIGKLDMAAPSLEEQTDLMTLLSWADETCATEGIRTAPNGWPKRGL
                     QVWANADYPKDARRARSYGAKGIGLCRTEHMFFEQVRLPIVQRMILAKTSQERTEALN
                     ELLPYQRADFEGLFEAMDDYPVIIRLIDPPLHEFLPSEESLLEEVVTMRVKGETQGLA
                     EKESLLASVRAMHESNPMMGLRGVRLSIVMPEIVEMQVRAIFEAAANMTLKGIEVKPE
                     VMIPLTGHVNELKLIQPRLVEIAKAVMQEKGTDFSYKFGTMIEIPRAAVTAKEIAEVA
                     EFFSFGTNDLTQMTFGYSRDDAERNFLVVYVEKGVLPKNPFQTIDQEGVGALMRSAVQ
                     AGRETRADLEVGICGEHGGDPASIEFCHTIGQNYVSCSPFRVPVARLAAAHAALKYAP
                     KKA"
     gene            complement(9939..>11686)
                     /locus_tag="PLL88_08070"
     CDS             complement(9939..>11686)
                     /locus_tag="PLL88_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ01559.1"
                     /translation="YDLASTDYQKASANLLAAQAYLELGLNDQAYTRLLDSVLNYPMA
                     YDSFTGLSILVSNGYPVNEYARGVVDYYAGSYDYAIQAFERYLAGNPEHDGSVYYFKG
                     LSHYYHEEYAQAIESYQLLIDYYPNNQFWDEAWEEIAFIYWNTPGDKYADIGNYQASV
                     QTRLDFVARAPQSNYAPYFLYIAGRALEYNGDLEQAAQVWARLINEYPTYEYSYRALF
                     LSGISYYRLGNYEEALKTFQRCLALSAVSEEKAAAYFWIGKTYQKMENVNDARPAWQQ
                     AESADPTDYYGIRAGDRLNDSDVLQITQPYEFGYDLDDERSEAEDWLRSTFNLSPETS
                     LVGLGELSSDARIMRGETYWELGLYELGSIQFEAVRAEQVTNPLNTYLLMNYFYDHGL
                     YKPAILACRDLLNLAGTDDLTSLAAPIYFTHIRFGAYFRNLVVSTANEENINPLIYFS
                     LLRQESLFEPFISSAAGAQGIAQIMPATAKDIVNRYGWPENYSVEDLLLAKVGITLGA
                     RYLRQQVDFLNGDMLAALAAYNGGPGNAYQWKELSNGDPDLFLEIIRFDETQTYLKQI
                     IEFLNIYKLIYTRIG"
BASE COUNT         3031 a         2614 c         3009 g         3032 t
ORIGIN      
        1 tattatggct cgcgctccaa tgcggtagat catctggaaa ctgccatgct gaatggtttc
       61 aaccgcattg aggtgggctg caacgctttg attgctgatg gattgaccgg ttcggattcc
      121 accgatattg agatcaatca aaaatatgta aaagctgcca aaattggcag tgccattgct
      181 gccgcagata tcgtcatcag tgtcagccac tttaagggtc atcaaggtac gggtttcggt
      241 ggcaccttga aaaatgttgg tatgggcagc ggctcaaggc gtggaaaaat ggaaatgcat
      301 gctgcatcaa agccaaagat ccgtgaagag aattgtgtgg catgcggaaa atgcatacaa
      361 cactgttctc aaaaagccat cgcgtacaac cagaacagga aagcccagat cgattattca
      421 aaatgcatcg gctgcgggca atgtgtggca agctgccatt atggtgctgc ggtggctctt
      481 tatgatgaaa attcagccac ggtcggtgca aaaatggctg agtacgccta tggcgttctg
      541 ctgggcaaac ccaatttcca catcagtttg atgatggata tcagccccga atgcgattgt
      601 tggaatatca atgatcaggc aattgccccc aatatcggta tggctgcttc ttttgatccg
      661 gttgctctgg atcgtgcttg cgtcgatctg gtcaatcagg cacctgccat taccaccagc
      721 gctcttttcg ataaaacccc ttatcaacct tcgcaagata aattcgatca catccatcct
      781 gatgtgacgt ggcgcgatac gctctctcat gccgaagaga tcggattggg aagccaatcg
      841 tatgaattgg tcaaaatcaa ataaaaagca aggagctgcc atattttggg tcagctcctt
      901 gtttgttggg tatgttccta ttctgtgata ggaggcgggg aactcgtggg ggtaagttcg
      961 agagtctctt cgaacacaac ataaggggtg ggtgtgggtt tccagccgct gttgttcttt
     1021 tcgcagaggg caattgcgtt ttcgacataa caacctttca ggctgccata tctcactatg
     1081 cttaaaagct ccaccagatc gccctgatta tctgcgagaa ggaggagcat tgtgcggttg
     1141 cttgtcgatt ttaataagaa aaacccaaaa tcactttgag aaacctctcc cagcccggtg
     1201 atgtagattt gtcctgcgcg taaggttgcc tgggtgggta tttgagcggt aattgattct
     1261 gtcgccgctg cgctggtttc agcggcagtg ggctgcggtg tgggcgtgtt tccggcttga
     1321 gagccgaatt gaatggctaa tccatcaaga tatttatgaa tggtttgatc tggcgggatg
     1381 agacctaata taatcaggtc gtagtcttcg tcatccgctt ccagaaattc cacagtgaga
     1441 tcttgttttg cgtaaagttc tttcacggcg tttatctgcg ccaaaagatc ctggtccaaa
     1501 actatccgct ctccgctgag aatgccgatc ggttttgtga aaaggttggg gaagtcatcg
     1561 aacgtcacct gccgttcgcc actcatgagg aactgtccga tgttcttgat taattggtag
     1621 ttatctgcaa cctgataata aggggaggtc aaaaaagtaa gatcgccgat cgcaaggacg
     1681 ttatttcccg ccagcgccac catgcagaga tctccgcccg tgtcggttaa ggaagagagg
     1741 gtgttggcat ctcctgcaag caagttcgtt tcataggaag aaatggaacg ggatgcgtaa
     1801 aaaacaaccc gctcaacgtt ttctgttaac tcattcttat ccaatggttc gagataaacg
     1861 ttgcggaaat ttccttcgtg atcgtaaacg ttataagcat aatcgttacg gaaaatgatg
     1921 tgatacggtt ccagcaatgt gttgacaacc ttaacgcttt gagcgcgtga gattgcatat
     1981 tctgatgaac tgcgggtcgg atcggcgatc acgagcagcc gccctcctcg cgctgcaaag
     2041 gtctgaattt gttggatctc atctccgctg aagtccatgg tgggggcaat ggaaatgaac
     2101 gccgcagcac cttttaaagt gctgttcagg tcttcgcctt cgtcgaaagt cacaatttct
     2161 gctcctcgat ctttcagctc atcgatcaac atctcgattt ccgtgatctg gtattggttg
     2221 gcgtgcgcca gatccaaaac cacactccct gttttcttta tctcccttgc gtttgaagcc
     2281 actgatgtcg ataatgtggg ttgaggtatt tcaaggctta aaaaatcggg agtttgaagt
     2341 tgtttgctcg gaatgtaaat tccctggtaa taccatactg tgcgtgcaat gattgggatc
     2401 aggaacaatc cgaccatgat caataagttt ttcgctttca tggaatttcg ctcccataat
     2461 cagggatata gagcagatag acgcctggtt ctctcggata tccggtggat gctccatttt
     2521 catcgagaat gaaaaaggat gatttctcat cgctggtttc accagccagt gcttcggaaa
     2581 tattgatgat tctataatgg gcgatgtgcg ccagttgggc agctttagca actgcgctgc
     2641 tctgagggcc cacctcgtcg atcagccccg ctttcgctgc ttcactggca gggtagagct
     2701 ctccccttaa gacttcctct ttgctcaatt tcagcttgtt ctcacgtcca agttttacca
     2761 cctggtaaaa actctctttg atcaattcga tatagcgcgt atatccttcg cgttgatttc
     2821 caaagagttt atagggtccg gtcgaaatgg tctcctccaa tactataggc gaggatggta
     2881 atgtgccaat gacccctacg ttcccaattc tggcggatgg atttgagatg atgtagtcgg
     2941 tgcccatggc gagatagaac gagccgctgg cagaaagtcc ttctaccata gccactacag
     3001 gtttggtctc gcgcagtttc atcaactcca gatagatcag ctctgtatcg ttgatcgtcc
     3061 cccctgggct gtccagaatc aacaccactg ctttgatttc gggatgcgaa cgtgcatatt
     3121 cgatctcttt cataaccctg atcgacattt ttgtatcgat ctcatcacgg atgtggatgg
     3181 accctatgac agggcgggga acaaggctgg caataagaat gcccagaacc agcggaagaa
     3241 gaaagattaa gatccgaggg tcatttttgg tcttacttga ctggctgttt tttttcatca
     3301 tcacctcaac agaacccttc aaggaagatt gtaccttaaa gggtttttat tttctctgga
     3361 tacctgcctc accttccgat tgatcatgcg cctgcttgtg acttgggaaa atgggtgagt
     3421 atagaggggt ctctcaaatt gtctaataaa atactaatac tatttgtttt tttccgtcct
     3481 acaatgaggg ttgaaacata ggtgcgagga tgaaagagta taagtattta attgtgggcg
     3541 ggggaataac tgctgcattt gccgtgcagg gtatccgcga gaaggacaaa gacggatcca
     3601 ttgcaatctt aaccaaagaa aactatgctc cttacagccg cccacctctt tcaaaagatt
     3661 tatggaaggg gaaacctgtt gaaagtatct ggctgcctgt tccagaagag aacgtagata
     3721 tcttgaccaa tcacacggtt atgcaattga ccctggcggc gaaaaaagtt ctcacccaaa
     3781 acaaacagga atttggcttt caaacgctgt tgctggcgac cgggagtgat tcaaaacgcc
     3841 tgcgtttttc agatgacgat attatctatt atcggaattt ttccgattac ctgaagctga
     3901 gtgatcttgc agagaaacgc aatgacttcg ctgttattgg agcgggtttc attggttcag
     3961 agattgccgc cgcccttgcc atgaaaagga agaaggtctt tcttcttgaa tccggggcgg
     4021 gtatcgggtg gaaaattttc ccgcaggcaa tcgtcgaatt tcttaacgct ctctatcgtt
     4081 cgaagggagt tgaagtgctt gaccacaccc tgattaaaga tatcgagaag cagggcgaac
     4141 ggcatcacgt gatgctcaaa aatggaaaaa ccatgccggt ggatggggtg atttcggggg
     4201 tgggagtgaa gccgaattcg ctgctggctg acaaagccgg tatctatgtg gaggacggga
     4261 tcaaggttaa ctcttatctt gaaacgtctt cccctgatat ctttgcggct ggggatgtgg
     4321 cagcatttca ttcttctgcg ctggataaaa cgttgcgtat cgagcataat gacaatgcaa
     4381 agaagatggg ctatttagcc ggcttgaata tggctggtgg gaatgaagag tatgactatc
     4441 ttcccatgtt ctattccgat ctttttgata ttggatatga ggctgtcggt ttgattgacg
     4501 tgcggcttga tccaaaagtg gtttggaaag aggaattcaa acggggtgtg atctattata
     4561 gaaaagacaa tgttatcaag ggtatcttgt tgtggaatga atgggataag gtagacctgg
     4621 cgcgcgaaat catcatgaag caaaaatgga tcgctgatga tgatctgatc aaattgatct
     4681 gaaaagcgca aagggaaacc tttacgtcct tgttcattga cttggaaaat ccgggaacca
     4741 aaaacctgag atgcggtagg gttcttttga atccagaacg agccgtaaag taagacttcc
     4801 gtccgaaaat tgaacagaaa aataagatat caaatacccc tcttcatagg ttgattgatc
     4861 gtatgtcagc gcttgatatt ctcccacggt ttgtgaaatg ctgtcgtgga gacttacaaa
     4921 ttggtcttcg ctgatggaag ataacatttt ttcggtgaag tctttgtgaa aacttgaaaa
     4981 atcattattc ttcaggctgg tgaaaaggtt ttcagccatc ggttggctga tctcctgaaa
     5041 attttctgga acggctgtgg tttttgtctg gcaggctgca agcagcagga tgatgaagag
     5101 agaaatacag atagatcgct tcatgttgaa tccctttcaa gtgaatccga aatcattcaa
     5161 ccttatttta actcatagcg agggtaagta ttgcgataca tgccacaaga aagagaccat
     5221 gacgaataag gcttttgaga acctgccctt cttttccaac caacccaacc gtagaacatc
     5281 ccacgatcac tttggctggc gcgaaaatgc tgccgatcgc tcctccggct gattgggcag
     5341 ccagaattag attcacgttc aacccaagca attccgccgt ttgcatatgc agcttggtga
     5401 aaagaatgtt ggaattattg ttgctgcccg atatgaaggc gcccagtgcg ccgataaagg
     5461 gcgtgattgc cgggtagagg gtggaaccca caaaaccgct caatcctttt gccagaatgt
     5521 tgatcatccc ggtatggctc atcagcacgg caatgccgac ggtaaagtat atggcaatgc
     5581 tggattgcaa tccgcttttt ttaacgctgg aaagaatttt tttataagcc tctttatcca
     5641 aaaggttctt cttcctgtag agaagaaatg cgatgatgga tgctcctaat aagatcattc
     5701 ccggatgggt gaaaagggtg atctcacgcc cgttttcagg agccgtgctc caaccaagat
     5761 tagaaacgat ctccggaaat gcataagtta attgaaagcg acttaaggga tcgttgataa
     5821 atgggatcag cttgatggca aacgagagca agatcagaat gaagtatatt gcaatggaaa
     5881 ggataaggga tggcttaaaa gggtctgtcg ttccctgaat ggctgctttt ttgtttttcc
     5941 cgctgctccc aatttttatc aacacgaaag cagcccccag cgcaaccaga gaagcgccgg
     6001 ttgttgcaac agtccacatt ccatttgtgg caagcagata ttggacgccg cctagaatgg
     6061 ctccgatggt gaagacaaaa gggagattct tcaagaccgc tttaaatcct gcataatatt
     6121 ggaccagcca tatcccgctg atcactgcca taacgcccag cagttttgct gtatcgggcg
     6181 caagggtaga accggcgata tcgctcaaag ccatcatggt ttgaaatgaa gatcccattg
     6241 acccaaacgt caccgcccaa ccgtgaccga tgctcgccat gataatcgca tcgatcgggt
     6301 caattcccaa tcccaccagc aggggggcgg ttactgctac cggaacgcca aaaccaccca
     6361 gcccttgcaa gaaagaggtg aacaaccagg ctaaaagcag aacctgcagg gtcttatttt
     6421 gtgtgaacac cgcaagcgca ttcccaagaa cctgaattgc ccctgcgtaa ttggaaatgt
     6481 ggaaaaaaag cagagccatc caaatgatca acaagacgtc taatgccagg aaaaccgctt
     6541 tgaaatgtgc ataaccaact aatgggatgt tcgctccgaa aaaggtgaac gtgatgacca
     6601 gcgtatatat ccagcttacg aatcctgctt tgctcccacc ccatttcttc ccgatcatta
     6661 aaaataagaa taaaagaata ggggaaaagg ataagagcca gtttacgaat gttgattcca
     6721 tggagagtcc ttgatgccta gaaattcttt tcaggcatta taaaggggtt tggatttaag
     6781 aaacgtgaaa cgttcaaaat cggtcaattc atttttgccc ctgcggtgat ggcacttgtt
     6841 ttgactgctc tcgatcagtt gattcacgca tagctcataa aaaagtcgga tcgcagccac
     6901 cattgtttga ataacgccac gttctgtgct gaagaaagca ggggctgcgt ttgcccggct
     6961 tgagctgctt cttttgaggg cgggtgtgca atgtgcttca accggaaccc tgttgctacc
     7021 gcaaggaccc gcagaggaat cataaatgtg aaaatttttg tatatggtaa aattgaaggt
     7081 taatgtgttt ttttcaaatt agtaattata ttttctggag gtaacaatga gcgataaaaa
     7141 gtgggtgtac cttttcaatg aggtagaagt cgcagaaaaa tacgctggca gttgggatgg
     7201 cgtgcgcggt ttgctgggtg gcaaaggtgc gaatatggca gaaatgatcc gcattggggt
     7261 gcccgttccg ccatttttca ctgtaacaac cgaaggatgt aatgcatata ttgccagtgg
     7321 cggcaaattt cctgaaggca tgtgggagca aatggttgtc gctttaaaag ccgttgaaaa
     7381 agcatccggc aaggaatttg gaaatcctaa gaatcctctg ctggtttctt gtcgttcggg
     7441 tgccaagatg tctatgcccg gtatgatgga taccgtcttg aacatcggtt tgaatgacga
     7501 gaccgccgga ggcatggtcg ctttgaccaa taacgagcgt tttgtatacg acgcttaccg
     7561 ccgcttgatc tccatgtttg gttctgtggt gttgggaatc tcggatgatg cgtttgaaga
     7621 cgttttgatc gatctcaaga aggaaaagaa agcgaactcc gacacagatc tggatgccgc
     7681 cgcgttgaag gaattggttg aaaaattcaa gaaagtcacc aggaatgcaa aaggcttcga
     7741 ttttccccaa gatccgttgg aacagatcaa attggctacc gaagcggttt tcaaatcctg
     7801 gaacagcaag cgcgctattg attatcgaag agctaccgat attcccgatg acctgggtac
     7861 agccgtcaat gttgtaacca tggtgtttgg aaatatgggt tggaattctg gaaccggtgt
     7921 ggcattcacg cgtaacccgt ccacaggtga aagagtgttg tatggtgaat acctgttgaa
     7981 tgcgcagggt gaagatgtgg tggctggaat tcgcaatacg gaaaagatcc agaccatggg
     8041 agaaaaactt cccgaagcca acaaacaatt cctcgaaatt gctgcaaacc tggaaaaaca
     8101 ttatcgcaac atgcaggatg tggaattcac ggtcgagaac gggaaactct ggatgctgca
     8161 aacccgtgac ggaaaacgca ctgccgctgc agcggtcaag atagctgttg acatggtgaa
     8221 tgaggggttg atctctaagg aagaagctat tcaacgggta actcccgctc aggtagatac
     8281 gcttttgcac ccccaattca cggatgaaac catcaagcaa gcccataagg atggacactt
     8341 ttttgccgat ggtgtggacg catcgcccgg tgcggcggta ggtcagatct attttgatgc
     8401 tgataccgct gaaaaaatgg caaaggaaga tgggcaagat gtcatcatgg tacgcccctt
     8461 taccaaaccg gatgacgtgc atggtatgct tgctgccaaa ggtattctta ccagtgaggg
     8521 tggcgctacc tcgcatgccg cggtggttgc tcgccagttt ggtgtgccct gtgtggttgg
     8581 cgcttcgatg atcaagatca acatggaaaa gcgtcagatg gaagcgggtg ggcggatcgt
     8641 taaagaaagc gaatggatat ccattgatgg cgggagcggc gaggcgttca tcggaaaact
     8701 ggatatggct gccccttctc tggaagaaca gacagacttg atgactttgt tgagctgggc
     8761 tgacgagacc tgcgccacag agggaatacg cactgcgccg aatggctggc cgaagcgtgg
     8821 tctgcaggtt tgggcaaacg ccgattatcc gaaagatgct cgccgtgcac gttcgtatgg
     8881 cgccaaaggc attggcttat gccgcacgga acacatgttc ttcgaacagg tgcgcttgcc
     8941 gatcgtgcag cgcatgatcc ttgcaaaaac ctctcaagaa cgaacggaag ccttgaatga
     9001 acttcttccc tatcagcgag cggattttga aggtcttttc gaagcgatgg acgattatcc
     9061 ggtgattatc cgcctgatcg acccaccctt gcatgaattc ctgccgtcag aagaatccct
     9121 tcttgaagaa gtggtcacca tgcgggtgaa gggagagacg cagggattgg cggaaaagga
     9181 atcgctcctg gcaagtgtac gtgccatgca cgaatccaat cccatgatgg ggttgcgcgg
     9241 tgtccgcctc agcattgtga tgcctgaaat tgtcgaaatg caggtgcgcg ctatctttga
     9301 agcagcagcc aatatgaccc tcaagggcat tgaggtaaaa cccgaagtca tgatcccgct
     9361 taccgggcat gtcaatgagt taaagctgat ccaaccgcgc ctggttgaaa tcgccaaagc
     9421 tgtcatgcag gaaaagggta cggacttctc gtataaattc ggcacgatga tcgagatccc
     9481 gcgggctgct gttaccgcta aagagatcgc agaggtggct gaattcttct catttggtac
     9541 caatgacctg acccaaatga cctttggcta cagccgggat gatgcagaac gcaacttcct
     9601 tgtcgtatat gttgaaaaag gagttctccc caagaatcct ttccagacca tcgaccagga
     9661 gggcgtcggt gccttgatgc gcagcgcagt ccaggcaggt cgtgaaaccc gggctgacct
     9721 ggaggtgggt atctgcggtg aacatggtgg cgacccggct tccatcgaat tctgccatac
     9781 tatagggcag aactatgtga gctgttctcc attccgtgtg cctgtggcac gcctggcggc
     9841 agcacacgct gctttgaaat atgccccgaa aaaagcctga tcgaattgaa aatcgagaaa
     9901 gaaaggcggt ctggtcaatt tccagaccgc tttttcattt atccgatgcg ggtataaatg
     9961 agtttgtaga tgttcaaaaa ttcaatgatc tgcttcaaat aggtttgtgt ttcatcaaag
    10021 cggatgatct caaggaacag atcagggtcg ccattggata gttctttcca ttggtaggca
    10081 ttgcccggac cgccgttgta ggctgccaat gctgcgagca tatcaccatt cagaaaatcg
    10141 acctgctggc gcaagtatct tgcacctaag gtgatcccga cctttgccaa caaaaggtct
    10201 tcaacgctgt aattctccgg ccaaccgtat cgattgacga tgtccttggc ggttgcgggc
    10261 atgatttgag cgatcccttg tgcccccgcg gctgacgaaa tgaagggttc gaataaactc
    10321 tcttgcctga gcagagaaaa atagatcagg ggattgatgt tctcttcatt ggctgtgctg
    10381 accaccagat tacggaaata tgctccgaag cgaatgtgcg tgaaataaat gggagctgcc
    10441 aggctggtca ggtcatctgt gccagccaga ttcaacaggt cgcggcaggc tagaatggcg
    10501 ggtttgtaaa gaccgtgatc gtaaaaatag ttcatgagaa ggtacgtgtt cagagggttg
    10561 gtgacctgct ccgctcgaac cgcttcgaat tggatgctgc ccagttcgta caatcccagt
    10621 tcccaataag tttctccacg catgatcctt gcatcagaac tcagctctcc caatccgaca
    10681 aggctggtct cgggagaaag attgaaagtg gaacgcaacc aatcttcggc ttcgctgcgc
    10741 tcatcgtcca ggtcgtaacc gaattcgtat ggctgcgtga tctgcaatac gtcgctgtcg
    10801 ttcaaacgat cgccggcgcg gattccgtaa taatccgtcg ggtcggcact ttcggcttgt
    10861 tgccatgccg gtcgggcgtc gtttacgttc tccatttttt gataggtttt cccgatccag
    10921 aaatatgcag cggctttttc ttcagacact gcggataaag ccagacagcg ctggaaggtc
    10981 tttagcgctt cctcatagtt gcccaggcgg taatagctga tgccggaaag gaataacgca
    11041 cgatagctgt attcgtaggt tgggtattca ttgatcaacc gagcccaaac ctgggcggcc
    11101 tgttccaggt cgccgttata ttccaacgct ctccctgcga tataaaggaa atagggtgca
    11161 taattcgact gaggtgccct ggctacaaag tcaagccggg tttgcacgga tgcctgataa
    11221 tttccaatat ccgcgtactt atctccgggg gtgttccagt aaatgaaggc gatctcttcc
    11281 cacgcttcat cccagaattg gttgttgggg taataatcga tgagcagttg gtagctttca
    11341 atggcttggg cgtactcctc atggtaataa tggctgagtc ctttgaagta ataaacgctt
    11401 ccatcatgct ccggattacc agccagatag cgctcgaagg cttgaatggc ataatcgtac
    11461 gaaccggcat agtaatccac aactccgcgc gcatattcgt tcaccgggta gccgttgctg
    11521 accagaatgg acagaccggt aaagctgtcg tatgccatgg ggtagttaag aacagaatca
    11581 agcagtcgcg tataagcctg gtcgttcaac cccaattcca aatatgcttg cgctgccagc
    11641 aggttcgccg aggctttctg atagtctgtg gaagcgaggt cgtagg
//
LOCUS       DAOSDS010000066        11595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2137_length_11595_cov_5.340381, whole genome shotgun sequence.
ACCESSION   DAOSDS010000066 DAOSDS010000000
VERSION     DAOSDS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2137_length_11595_cov_5.340381"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..1011)
                     /locus_tag="PLL88_08075"
     CDS             complement(256..1011)
                     /locus_tag="PLL88_08075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ01560.1"
                     /translation="MKLYFEEYGMGLPVVFLHGYPLDHTIWNPLIPLLETKIQLVLPD
                     LRGHGKSPAPSGLYAMQDLAEDVKDLVDLLGFSHVLLVGQSMGGYVALQFAHSYPKNV
                     LGLILVASHPYPDDEEKREGRYASVRRVQKEGVAKALAEFPAKLSPFPAVQAYTKEII
                     STARADGVIGCLQAMADRADRSEVLSMAQYPTAIILGEKDLFISPDLREKMLAQFPET
                     GLTTLDNAAHMLMMEEPQAVSQTIEMVVSKIEE"
     gene            complement(1019..2524)
                     /locus_tag="PLL88_08080"
     CDS             complement(1019..2524)
                     /locus_tag="PLL88_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01561.1"
                     /translation="MKQFAMQENEFFQTVLFFLRSLENTRIYLVGGAVRDALLGKPSH
                     DFDFIVEGDALATARKLANHIKGAFYVLDSSRNYARVIAHDSAGVRNILDFAPLYQNE
                     LTLDLQSRDFTVNAIAWEISSERRALTDPLHGMADLENGVLKLCSDGAFASDPVRMIR
                     AARFAVEYKLELETPLRAQVRASASKLGAVSEERRRDELFKILENERVATALEILRDM
                     HVLEWVYPEVYKLVGFQQGNPHRFDGWQHTLKTVHYSEGMLNFIQNGEKGDHSSVWML
                     DVFRLLDEYAADLKRALSKSPVVERSLRSLILFSALHHDVGKPLDRGEVVGNRVRYAH
                     HAALGAKAALRCAQRMALSKAESQWVEQFVSHHMLLHDMDIHASEMEQRVFLYRFFQS
                     AGRASPFVALFSLADLLATYDDALVEERWRAGLVRCAKALNGWFREHHALAAPEPLLD
                     GDELQKKFHLKPGKQLGELLDGLREAQAAGKVKSKSQAIKWVDQWLQKTKD"
     gene            complement(2521..3084)
                     /gene="hpt"
                     /locus_tag="PLL88_08085"
     CDS             complement(2521..3084)
                     /gene="hpt"
                     /locus_tag="PLL88_08085"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOJ01562.1"
                     /translation="MVPYEEYLKEKLISEEVLQKRIAELGAAISHDYANSSNLLLICI
                     LRGGIMFLTDLMRHITVPHAIDFMAVSSYGEGKRESQGSVYIKMDLMTDIGGRDVIIV
                     EDIVDSGYTLSSVLQLLSTRKPASLNICTLLDKAERRQVEVPIKYTGFVIPNKFVFGY
                     GLDLDELFRNLPFIGVVDLKKYDAKKS"
     gene            complement(3089..3661)
                     /locus_tag="PLL88_08090"
     CDS             complement(3089..3661)
                     /locus_tag="PLL88_08090"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOJ01563.1"
                     /translation="MDHNSGWLEVVCGSMFSGKTEEMIRRIRRAVIARQQVQVFKPAI
                     DNRYEVQKVTSHAGLDFDAIPISSSAELWSNLRNDTTVVGIDETQFLDDGIVDVVEKM
                     VERGIRVIATGLDMDFRGEPFGCMPILMAKAEKVDKVQAICMVCGEPATRTQRLVDGK
                     PAHYDDPIVIVGAAEMYEARCRKHHLVPRG"
     gene            complement(3774..4058)
                     /locus_tag="PLL88_08095"
     CDS             complement(3774..4058)
                     /locus_tag="PLL88_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HOJ01564.1"
                     /translation="MQARQEYIEDKEQKAAEKVSPTRPIAELEVGKRATDALGEAGIK
                     TVGDLLTKLEAGETPILEIPGVGRKTLIDIKKKLRQFGYTIPEAAEEIKV"
     gene            complement(4047..4250)
                     /gene="rpmE"
                     /locus_tag="PLL88_08100"
                     /pseudo
     CDS             complement(4047..4250)
                     /gene="rpmE"
                     /locus_tag="PLL88_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            complement(4285..4542)
                     /gene="rpmA"
                     /locus_tag="PLL88_08105"
     CDS             complement(4285..4542)
                     /gene="rpmA"
                     /locus_tag="PLL88_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOJ01565.1"
                     /translation="MAHKKGGGSTRNGRDSNSKRLGVKRFAGEQVISGNILVRQRGTK
                     IKPGDNVGLGKDYTIFSKVDGVVVFDQLPNGQKKVSVHPND"
     gene            complement(4566..5075)
                     /gene="rplU"
                     /locus_tag="PLL88_08110"
     CDS             complement(4566..5075)
                     /gene="rplU"
                     /locus_tag="PLL88_08110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOJ01566.1"
                     /translation="MLYAIFESGGKQFKAVEGSYVEVDRLPTEEGKKVSFDKVLLLVN
                     DKETQVGTPFVKGAAIDATVAEHFKGKKVYIFNYRPKKRYRVKTGHRQNYTRLMVNSI
                     VFSGKPAKGATEEKAAVSAEKSVKPVEKKPSAAKTASSKKSTTQKKTTASVAKTGQKT
                     NKPAKADKK"
     gene            complement(5217..7124)
                     /gene="selB"
                     /locus_tag="PLL88_08115"
     CDS             complement(5217..7124)
                     /gene="selB"
                     /locus_tag="PLL88_08115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOJ01567.1"
                     /translation="MMKYVIATAGHVDHGKSALVKVLTGIDPDRLMEEQQRQMTIDLG
                     FAWFTLPSGDEVGIVDVPGHRDFINNMLAGVGSIDAVMLVVAADEGIMPQTLEHMMIL
                     DLLEIKRGLIVITKTDLVREPAWLEMVENDIRDAVRGTVLQDFPLVQVSAVTRDGIQG
                     LVGCIQTTLSQSGYAEKHGDAHLAVDRVFSIKGFGTVVTGTLRGGFLKTGEEIEILPV
                     GKISRIRNIETHKQKLTIAEPGSRVALNLTGVDVQEIDRGSVVVRPKSVKPSMRLDAQ
                     VRIIASSSRGLKHNDQVKLYHGTSEILARVRTLGLDVIQRGETGFVQLELSAPLVCWK
                     GDHFIIHRPSPQETIGGGQIINPAPDRRYKRYAERTLTNLNAQRVGSVWDIFALLLEG
                     KFLVSEAKLEDIFLKEGLVYQDEVEMLRSHAEFVEFSTICSQKNMLRFVTTRQKLYDL
                     SKVINSALYDTYQRSAIAPGLLISELSKKFGMQDCVLQSLLEKELLDEDLAFAKRYVF
                     LKGRAIQFPDKDLHKIERLNSIIEREPYAPPSLEEIQDIVGNELLQALLFTGELVQVE
                     DSIAFRENEFNAMKAQLIRFLERHGEISLAQYRDLLHTSRKYALAFLEYMDRSGVTER
                     VGEVRKLKTSG"
     gene            complement(7142..8299)
                     /locus_tag="PLL88_08120"
     CDS             complement(7142..8299)
                     /locus_tag="PLL88_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01568.1"
                     /translation="MNLETIVKRMDDLEERIKNFDNLFKTFQDKLADLDTRVANLPAE
                     YRDIKDEVAQLKKSVANAGNVDKALLQIRKDTNEKIIALEKESKKQQEQQSKLQQTDI
                     KALSAKIDLLDTKIKSELDKRIRHYVDEDSHLLATVEKIEQSVNTKLRTDDDIRRTHE
                     IQKRDIQNMSKRIEIIADDMNQFRNQQVEITEKIGVLLESTKRAEVQIAELKSTETQR
                     KMSQTAFIEQQEVAHKQREIYFNEIQRRVTEEFQKISLMLERLGQKEKELNQTGNNLD
                     EMTTSYERRLREVTELYQLFEAKYQKEWGSFKNEIEKDWSNFSLINDEKQSALTARMD
                     DLRSNIVLLEDQTNDLKELLGLMSSEIQKGMLSLMKMATAWKDAFDHIEGK"
     gene            complement(8322..9716)
                     /locus_tag="PLL88_08125"
     CDS             complement(8322..9716)
                     /locus_tag="PLL88_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOJ01569.1"
                     /translation="MNLPFIFIVIPLLVSAASLIIRKRGILYRSLLSLCTFLFGLGAF
                     IMAALSSDASTALSINEEFLILGRSLHISYTDLPLIGLIYFITTLWIVTTICFESNTF
                     FPALSVSYTGFLVASISIDPFLYSTLIFFLANGIFLIALSKGEYRAIAGRMRFLLFQL
                     LSVFLILLAGWILAGGEIAPVEEDQLFISSVLLGCGVALWLGAFPFHTWIPLLVEEVF
                     FLPFGFMMTLMPISGILMLLRFVDNFAWLREYSAFYPAVLYLGVVMILLGGIGSFFQK
                     SLKRVLSFVFIAGIGMLLSSVGFVPYASTDLVGSWLIPIAVGFWSISLAYVELVGSQS
                     GSNIEDLKGKFIEKPLHAILLISGLFTVSGLPLFSGFAPNILMLHISVGINRVIQFCI
                     LGGEFFLCMTAIRLMLRLFIFSPPDKMRIPDKIKIDRILVGLIILWNIFQGIFPSLIF
                     EPIFKALSKIFPLI"
     gene            complement(9713..10282)
                     /locus_tag="PLL88_08130"
     CDS             complement(9713..10282)
                     /locus_tag="PLL88_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01570.1"
                     /translation="MADVLFLILIAVIILSITTMALTDSARVTLAFLGVLYLCSFVLI
                     LQVWPLGLAAVHLLTGLLTVIIVNFYCSKITWQPSTPRNALDILLIVFVGVVAFALAP
                     QVVVYLTEPLQHLVIGFFLFITGLLQAGTSQEEFRVFIGLLILTLGFQVIYAPLEGSA
                     LVTALISLVQIALSLVGARILIKNREELR"
     gene            complement(10285..>11595)
                     /locus_tag="PLL88_08135"
     CDS             complement(10285..>11595)
                     /locus_tag="PLL88_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01571.1"
                     /translation="CISIYLLVFLSRQYHEREKLSLPLEKIIFVFCCVIFGLLTLSLH
                     KEFALTTILFCLDIFRLVIRVIRNKELFSSQRDTLSLLLRLVSIVLMVYLAARVDPEG
                     YEAGLFSIFPLVVTVIASRIAADFIDQISADQFTGENLHGWLMFLDFVTMVSLLILLP
                     EQTGNTAGILLYSLVFFGMLLLLLTYLWIFRSRSHENRIWIFFFAMSLTLFFFLLGIR
                     QELLFLMLPICFLMINSPLHDGKRGIEIVLLICEGIFLLGFTFSPFYLLNKNLLNVPN
                     LLPFKYFALIMEGVYLAGWVITQRRFELQGSETEKGSASRELTPAMFVGGLCLIIYKG
                     FFPLDGFNQIAWFAFSPLVMYLLVPIHALLKRGYHAEKNYDPLQAHAQATRFVDTVMR
                     VFLVVGDVIRQLFEAISTLLEREAGLIWAIIFLVLLLTLFKGLS"
BASE COUNT         3249 a         2953 c         2378 g         3015 t
ORIGIN      
        1 gagcgtattg cagtcagcca aaaatggttt gccgttcaat tcctgaattc ttttcacaac
       61 cctggctatg tatccggggc ggatatatgc catgtttccc cgttcagcaa atgtgatctt
      121 gatcgccacc agtttatctt tgaaatcgat gctatccatt tttgcgcgtc tcatgaccgc
      181 atccaattat ccagcaaccc ataatcaggt aatacgcgca tatccgtaaa aaataccttt
      241 gaagccatcc tatcttcact cctctatttt gctaacaacc atctctattg tttgactgac
      301 cgcttgaggt tcttccatca tcagcatatg cgccgcgtta tccagagtgg tcaaaccagt
      361 ttctggaaac tgggcaagca tcttctctct taaatctggg gagataaaga gatctttctc
      421 acctagaatg atcgcggtgg gatactgcgc catcgatagc acctcactgc ggtcagcgcg
      481 atctgccatc gcctgcaaac agccaatcac accatctgca cgggcagtgc tgataatctc
      541 cttagtataa gcctgaactg ctggaaaagg cgataatttt gccggaaatt ctgccaacgc
      601 ttttgccaca ccttctttct gaactcttcg aacggacgca tagcgacctt ctcgcttctc
      661 ctcatcatcg ggatagggat gggatgcaac caatatcaat cccagcacat ttttggggta
      721 ggaatgcgca aattggagag ccacataacc gcccatggat tgtcctacca gcaagacatg
      781 ggaaaatcca agaagatcga ccaggtcctt cacatcctct gccaaatctt gcatagcata
      841 aagacccgaa ggtgccggtg atttgccatg accgcgcagg tcaggtaaga cgagctgaat
      901 ttttgtctcc aaaagtggta tgagcggatt ccagattgta tggtcaaggg ggtaaccgtg
      961 caaaaaaaca accggcaagc ccatcccata ttcctcaaag tacagtttca tcgtattgtc
     1021 agtcctttgt cttttgaagc cactgatcaa cccatttaat ggcttgtgat tttgacttaa
     1081 cctttcccgc agcctgcgct tcgcgcaatc catccaacaa ttctcccaat tgcttgccgg
     1141 gtttgaggtg aaatttcttt tgcagctcat ctccatcaag caatggttcc ggtgcagcca
     1201 gagcatgatg ctctctgaac caaccgttca acgcttttgc gcagcgcacc aaaccagccc
     1261 gccaacgctc ttcgaccaac gcgtcatcgt aagtagctaa aagatcagcc agagaaaaga
     1321 gcgccacaaa cggactggca cgaccggctg attgaaaaaa acgatagagg aagacgcgct
     1381 gttccatctc gcttgcgtga atatccatgt cgtgcaacag catatgatgg ctaacgaatt
     1441 gttccaccca ttggctttcg gctttgctga gagccatgcg ttgtgcgcaa cgcagcgccg
     1501 ctttggctcc caatgcagcg tggtgggcat agcgcaccct attaccaaca acctcccccc
     1561 gatccaacgg tttgcccaca tcgtgatgga gagcggaaaa caagatgaga gagcgaagag
     1621 aacgttcaac caccggcgat tttgaaagtg cgcgcttgag atcagcggca tactcatcca
     1681 acaagcggaa gacatccagc atccatacgg aagaatgatc gcccttctct ccattctgaa
     1741 tgaaattcaa catcccttca gagtagtgca cagttttcaa tgtgtgctgc caaccatcaa
     1801 atcgatgagg attgccctgt tgaaaaccca ccagtttgta tacttcggga tacacccact
     1861 caagaacatg catatcgcgg aggatttcca gcgcagttgc aacacgctcg ttctcaagga
     1921 tcttgaataa ttcatccctc cttctttctt cagaaacagc gcccaatttc gaagcggatg
     1981 cacgaacttg agcgcgaagc ggcgtctcaa gttccaattt gtattccacc gcaaaacgcg
     2041 ctgcccgtat catgcgaacc gggtcgctgg caaaagcacc gtccgaacac agtttcagga
     2101 ccccattttc caggtctgcc atgccatgca aaggatcggt caatgcacgc ctctcagaag
     2161 agatctccca ggcaatggca ttgacggtga aatcacggga ttgaagatca agggttagct
     2221 cattctgata gagaggtgca aaatcaagga tgttccgcac accagcggaa tcgtgggcaa
     2281 tcacacgcgc ataattacgg gaactgtcca gaacatagaa cgcacccttg atatggtttg
     2341 ccagttttcg agcagttgcc agcgcatcac cctccacaat aaaatcaaaa tcatgagagg
     2401 gtttccctag caaggcatcc cgaaccgcac cgccaaccaa atagatacgc gtattttcaa
     2461 gagaacgcaa aaagaaaaga acggtttgga aaaattcatt ctcctgcatt gcaaattgct
     2521 tcatgacttt tttgcatcat atttcttgag atcgaccacg ccaatgaaag gcaaattgcg
     2581 gaacaattca tccaggtcaa gcccatatcc aaacacgaat ttgttcggaa taacaaagcc
     2641 ggtgtatttg atgggcacct caacctgccg acgttccgct ttatcaagca gggtacaaat
     2701 attcaaactg gcaggttttc tggtggataa taattgaagc accgacgaaa gggtatagcc
     2761 gctatcaacg atatcttcta caataataac gtcccgacca ccgatgtcgg tcatcagatc
     2821 catcttgata tacaccgatc cctgcgattc tcgttttccc tcaccatagg aagagaccgc
     2881 catgaaatca atggcatgcg gtaccgtaat atggcgcatg agatcggtca aaaacatgat
     2941 cccaccgcgt agaatgcaaa tgagcagcaa attggaagaa ttggcataat catggctgat
     3001 cgccgcccct aattccgcaa tccgcttctg caacacttct tcggagatca gtttttcctt
     3061 taaatattct tcatacggga ccatttttct atcctctggg cacaagatga tgttttcggc
     3121 agcgtgcctc atacatttca gccgctccaa cgatcacaat cggatcatca taatgggctg
     3181 gctttccatc aaccagacgt tgggtgcggg tagcgggttc tccgcagacc atgcagatcg
     3241 cctgaacctt atccactttt tcggcttttg ccataaggat gggcatgcaa ccaaatggtt
     3301 ctccacggaa atccatgtcc aaccccgttg caatgacccg aattcctctc tccaccatct
     3361 tttccaccac atccacaatc ccgtcatcga ggaattgcgt ttcatcaatg ccaaccaccg
     3421 tcgtgtcatt tctcaaatta gaccataatt ctgcagagga agaaatcggg atggcgtcaa
     3481 aatccaaacc ggcatgggag gtcactttct gtacctcata gcgattatcg atcgcgggtt
     3541 taaaaacctg aacctgttgt ctggcgatca cagcccgacg tatgcgcctg atcatttctt
     3601 ccgttttccc gctgaacatt gatccgcaca caacttccaa ccaaccagaa ttatgatcca
     3661 tagcttacct catccaagca tttattattg attgtacatt atctcgtaat accagcggtt
     3721 aaaatgaaaa agagcagatg aatttccatc tgctcttctc aaacttgcag aaattaaact
     3781 ttgatctctt cggcagcttc agggatggta tacccgaact ggcgtaattt cttcttgata
     3841 tcaatcagag ttttgcgacc cacaccaggg atctcgagga tgggggtctc gcctgcttcc
     3901 agtttcgtaa gcaggtcacc aaccgtttta atgccagctt cgcccaacgc atccgttgcg
     3961 cgttttccga cctcaagttc agctattggg cgggtaggag aaaccttttc ggctgccttt
     4021 tgctctttgt cttcgatgta ttcctggcgc gcctgcagtt tcttatagaa tctgtcaacc
     4081 tgaccttcaa tatcaatcaa gcgagcctgt tcaccggtaa agaaaggatg gcatttcgaa
     4141 cagacttcgg tacgaatttc tggttttgtg gatccggttt tccaggtatt cccacacgca
     4201 caggtaactg tcgcttcagg ataccacttt ggatggatgt cttttttcat ggtgtttcct
     4261 cattccattt caatcatcca acgatcaatc gttgggatgc acgctcactt ttttctgacc
     4321 attggggagc tgatcgaaaa cgaccactcc atcaacctta gaaaagatgg tgtaatcttt
     4381 tcccagtcca acattatcac ctggtttaat tttcgttcca cgctgacgaa ccagaatgtt
     4441 tcctgaaatg acctgttccc ctgcaaaacg tttcacaccc aaacgcttcg aattgctatc
     4501 gcgaccattc cgagttgagc cgccaccttt tttatgagcc attgttcact tccttcctgc
     4561 ttcttttact tcttatcagc tttcgctggt ttgttggtct tctgtcctgt ttttgcaacc
     4621 gaagctgtcg ttttcttttg cgtcgtcgat ttttttgaac tggcggtttt ggcagcggag
     4681 ggtttcttct ccaccggttt aacggatttt tccgcgctta cagccgcttt ttcctccgtt
     4741 gcaccttttg ctggcttgcc ggaaaaaacg atggaattca ccatcaagcg cgtatagttc
     4801 tgccgatgac cagtttttac acgataacgc ttcttcggac ggtaattgaa aatatatacc
     4861 tttttccctt tgaagtgctc cgccactgtt gcatcaatgg ctgctccctt tacaaaggga
     4921 gtaccgacct gcgtttcttt gtcatttacc agaaggagaa ctttatcgaa agaaactttt
     4981 ttgccttctt cggtgggcag gcgatctact tctacatagc tcccttccac agccttaaac
     5041 tgcttcccac cactttcaaa tattgcatat agcatgtgtt acgctcctca accttcactt
     5101 caccggatat tgtgcgtttc ctacgcccaa atgcggggaa gttggggata catgaattta
     5161 ccccacttct ctttgcgggg caagctgctg aattatacag ttcctttttc gtgctgtcaa
     5221 cctgaagtct tcagcttacg cacttccccc actctttccg tcaccccgct gcgatccata
     5281 tattccagaa aagccagcgc atattttcgg ctggtatgca gcaggtcacg atattgcgcc
     5341 agggatattt caccatgtct ttcaagaaat cggatcagtt gagctttcat agcattgaac
     5401 tcattttcgc gaaatgcgat ggaatcctcc acctgaacca gttctcccgt gaaaagaagc
     5461 gcctgaagaa gttcattccc aactatatct tgaatttctt ccaacgaagg cggcgcataa
     5521 ggttctctct ctatgatgct gttcagccgt tcgatcttgt gcaaatcttt atcggggaat
     5581 tggatcgctc gacctttgag aaatacgtat ctctttgcga aagccagatc ttcatcaagt
     5641 agttcttttt ccaggagaga ttgcagaacg cagtcttgca tgccaaactt tttgctcaac
     5701 tctgagatca gcagtcccgg agcaatcgcg gatctctggt aggtgtcata caacgcagaa
     5761 ttgatcactt ttgaaaggtc gtacaatttc tgccttgtag tcacaaaccg aagcatattt
     5821 ttttgcgaac aaatcgtcga aaattctaca aattcagcgt gagaccggag catctccact
     5881 tcatcctggt aaacaagccc ttctttcaga aaaatatctt ccagtttcgc ttctgatacg
     5941 agaaatttac cctccaggag caaggcaaaa atatcccaca ctgaacccac acgctgcgca
     6001 ttgagattgg tcaaggttct ttcagcatat cttttatacc gtcgatcggg agcgggattg
     6061 atgatctgcc cacccccaat cgtttcttgc ggtgaaggtc ggtgaatgat gaaatggtcc
     6121 cctttccaac acaccagcgg ggctgataat tctagctgca caaaccctgt ttcaccgcgt
     6181 tggataacat ccagccccaa cgtacgcacg cgggcaagga tctcgctcgt tccatggtac
     6241 agtttcacct ggtcattgtg tttcagccct ctacttgatg aagcaatgat tcgcacctgt
     6301 gcatccaacc gcatgcttgg tttgacagac ttgggtctta caacaacact gcctcgatca
     6361 atttcttgca catccacccc ggtgagattc aatgccaccc tgctccctgg ttcggctatc
     6421 gttagctttt gtttatgtgt ctcgatgttg cgaatccgac tgatctttcc caccggaaga
     6481 atctctattt cttcgcccgt tttcaggaaa ccaccgcgta gtgttccggt aaccaccgtc
     6541 ccaaatcctt ttatcgagaa cacccgatcc actgccaggt gcgcatcacc atgtttctcg
     6601 gcgtagccgg actgagacag ggtcgtttga atgcatccca ccagaccctg gatgccatcg
     6661 cgggtcactg ctgagacctg caccaatgga aaatcctgca aaacagtccc tcggacggca
     6721 tctcgtatat cgttctcaac catttcaagc cacgcaggct cccggacgag atcagttttc
     6781 gtgattacga tcaaccctct tttaatctcg agcagatcaa ggatcatcat gtgctcaagc
     6841 gtttgaggca tgattccctc gtcagcagca acaaccaaca tcaccgcgtc aatacttccc
     6901 acgcctgcca gcatattgtt gataaaatcg cgatgtccgg gcacgtccac aataccaacc
     6961 tcatcccctg aaggcaaagt gaaccatgcg aagcccaggt cgatggtcat ctggcgctgt
     7021 tgctcttcca tgagacgatc cgggtcgatt ccggtcaaca ctttaacaag cgcagatttg
     7081 ccgtgatcaa catgcccggc agttgctatt acgtatttca tcatgcctgt cttcgcatcg
     7141 attattttcc ctcgatgtga tcaaaagcat ccttccaagc agtagccatt ttcataaggc
     7201 ttaacatccc cttttgaatc tcactgctca tcaacccaag caattctttc agatcgtttg
     7261 tctgatcttc caagagaacg atatttgatc tcaggtcatc catgcgagcg gttaatgcag
     7321 attgcttttc atcgttaata agcgaaaaat tggaccaatc tttttcaatc tcattcttaa
     7381 agctgcccca ctctttttga tattttgctt cgaaaagttg atagagttct gtcacttctc
     7441 tcaatcggcg ctcataggat gtggtcattt catcgaggtt gttgcctgtc tggttcagtt
     7501 ctttttcttt ctgacccagc cgttccaaca tgagcgaaat cttctgaaac tcttcggtga
     7561 ctctgcgctg gatctcattg aaatatatct cgcgttgttt atgggcgact tcttgctgtt
     7621 caataaacgc cgtctgactc atttttcttt gcgtttctgt gctcttcaac tctgcaatct
     7681 gaacttcagc tcgtttggtg ctttctaaaa ggacaccgat cttttccgta atttcaacct
     7741 gctggtttcg aaactgattc atatcatcgg cgatgatctc gattctcttg ctcatattct
     7801 gaatatcccg cttttggatc tcatgagtac ggcgaatatc gtcatccgtt ctcagtttcg
     7861 tattaacact ctgttcgatt ttttcaaccg ttgcaagcag atgagaatct tcatctacgt
     7921 aatgtcgaat tcttttatcc agctctgatt tgatttttgt gtccaggagg tctattttcg
     7981 ccgacagcgc ttttatatcg gtttgttgga gtttactttg ctgttcctgc tgttttttgc
     8041 tctctttttc cagagctata atcttttcat tcgtatcttt gcgaatttgg agcaatgctt
     8101 tatcgacatt gcccgcattc gcaacactct tttttaactg agcaacttca tccttgatat
     8161 ctcgatattc tgcgggtaaa tttgctactc ttgtatccag atcggcaagt ttatcctgga
     8221 aggtcttaaa aagattatcg aaatttttaa tccgctcttc gagatcatcc atccttttta
     8281 caatcgtttc caggttcatt tttcaccagt tcctatattt tttagatcaa tggaaatatt
     8341 ttactgagcg ccttgaatat tggttcgaat attaaagaag gaaagattcc ttgaaagata
     8401 ttccaaagga taatcagccc gaccaatatt cgatctattt ttattttatc cggtatcctc
     8461 attttatcag gaggagaaaa tatgaaaagc cgcaacatca gccgtatggc ggtcatgcat
     8521 agaaaaaact ctccgccaag gatacaaaat tggataacgc gatttatccc taccgaaatg
     8581 tgtaacataa gaatattggg agcaaatcct gaaaaaagcg gcaatcctga aactgtaaaa
     8641 agtccactga tcagcaggat ggcatgcaga ggtttttcaa taaacttccc tttcagatct
     8701 tcaatgtttg accctgattg agaccccacc aattccacat atgcaagaga aatcgaccag
     8761 aacccaacag caatgggaat caaccaacta cctacaagat ctgtagaagc gtaaggaaca
     8821 aaaccaacac tgctcaataa catccctatt ccagcgatga acacaaacga gagaacgcgc
     8881 ttaagcgact tctgaaagaa cgatccgatg ccacccagaa ggatcatgac aactcccaga
     8941 tacaatacag caggataaaa ggcgctatac tctcgcaacc aggcaaaatt atccacaaat
     9001 cgcaacaaca taagaattcc tgaaattggc atcagggtca tcatgaaacc aaatggcaag
     9061 aaaaaaacct cttcaaccaa caaaggaatc caagtatgga aaggaaacgc acctaaccat
     9121 aaagccacac cgcaccccag caataccgat gagataaaaa gctgatcttc ctctacaggt
     9181 gcaatttcac cacctgccag gatccaacct gcaagcaaaa taagaaatac ggataacaat
     9241 tgaaataata aaaagcgcat acgcccagca atcgccctat actctccctt tgagagcgct
     9301 ataagaaaaa tgccattcgc aaggaagaat atcaaggttg aatagagaaa ggggtcgatg
     9361 gaaatcgacg caacgagaaa acctgtatag gaaacgctca gcgccggaaa aaaggtattc
     9421 gattcaaagc agatggtagt aacaatccat agcgtagtga tgaagtatat taacccgatc
     9481 agaggcagat cagtatacga aatatgcaaa cttcgcccga gaatcaagaa ttcttcgtta
     9541 atggaaagag cagtgctggc atcagaagat agtgcagcca taatgaacgc gcccaatcca
     9601 aaaaggaacg tgcacagaga caacagagaa cggtatagaa tgcccctttt tcgaataatc
     9661 aaggatgcgg cagaaacaag caatgggata acaataaaaa tgaacggaag gttcatcgca
     9721 attcctcacg attcttaatc aggatacgcg cgccaaccaa tgacaatgcg atttgaacca
     9781 acgaaatcaa agcggtaacc aatgcggacc cttccagcgg agcataaatc acctgaaagc
     9841 ccaaagtgag gatcagcaag ccaatgaaga ctcgaaattc ctcctgcgag gtaccggctt
     9901 gtagaagccc cgtgataaac aggaaaaacc cgatcaccaa gtgctgaagg ggttccgtta
     9961 gatatacaac tacctgcgga gccaacgcaa aagcgaccac accaacgaaa acgatcaata
    10021 aaatatcaag ggcattacga ggtgtactgg gttgccaggt tatttttgag caataaaagt
    10081 tgacgatgat caccgtcaac agaccggtca ataaatggac cgccgccagc ccaagcggcc
    10141 acacctgcaa aatcaagaca aaactgcaaa gatagagaac ccctaaaaaa gccagagtaa
    10201 ctcgagcaga atctgtcaac gccatggttg taatggaaag aatgatcaca gcaatcagaa
    10261 tcaagaacaa tacatcagcc atgattaaga caatcctttg aataacgtca gcaataagac
    10321 caaaaaaatt atcgcccaaa tcaaaccagc ttccctttca aggagagtgc tgatcgcttc
    10381 gaatagctgc ctgatgacat ctcccacaac cagaaaaaca cgcataacgg tatcaacaaa
    10441 acgcgttgcc tgggcgtgcg cttgcaatgg atcgtaattt ttctcggcat gatagccacg
    10501 ctttagaaga gcatggatgg gaaccagcaa gtacatgacg agcggggaaa aggcgaacca
    10561 tgctatctgg ttaaagccat ccagcggaaa aaagccttta taaataatca gacagagacc
    10621 accaacgaac atcgctgggg taagttccct cgacgcggac cctttttccg tttcagatcc
    10681 ttgcagctca aagcgtcttt gtgtgatcac ccatccagcc agatagaccc cctccatgat
    10741 gagggcaaaa tatttaaacg gcaaaagatt cggaacattg agcaggttct tattcaacag
    10801 gtagaaggga gaaaatgtaa atccaagcag aaatattcct tcacaaatca acagaacaat
    10861 ttcaattcct cgctttccat cgtgtaatgg cgaattgatc atgagaaaac atataggcag
    10921 catcaagaaa agaagttcct ggcgaattcc cagcaagaaa aagaaaagcg tcagcgacat
    10981 ggcaaagaag aagatccaaa tgcgattttc gtgggaacgt gaacgaaata tccagagata
    11041 tgtcagcaaa aggagcagca ttccaaaaaa cacgagggag tagagcaaaa ttcctgcagt
    11101 gttgccagtt tgttcaggca gcaggataag aaggctgacc atggttacaa agtcaagaaa
    11161 catcaaccag ccatgcaaat tctcgcctgt gaactgatca gcgctgatct gatcgatgaa
    11221 atctgccgcg atcctggaag ctatgaccgt aacgaccagg ggaaagatgg agaacaatcc
    11281 ggcttcatac ccctcggggt caacccgggc tgcaaggtaa accatcagga cgatggaaac
    11341 caagcgcaac aacagcgaga gcgtatctct ctgagaggaa aacagctctt tgtttcgtat
    11401 gacccggatt acaagccgaa aaatatccag gcaaaagagg atagttgtca atgcaaattc
    11461 tttatgcaaa cttaaggtaa gtaacccaaa gattacacaa caaaaaacaa aaataatttt
    11521 ctccagaggc agagatagtt tttcccgctc atgatattgc ctggatagaa aaacaaggag
    11581 gtagatggag atgca
//
LOCUS       DAOSDS010000067        11502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2158_length_11502_cov_4.842579, whole genome shotgun sequence.
ACCESSION   DAOSDS010000067 DAOSDS010000000
VERSION     DAOSDS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11502
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2158_length_11502_cov_4.842579"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PLL88_08140"
     CDS             complement(<1..1061)
                     /locus_tag="PLL88_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01572.1"
                     /translation="MEKQTQNKKKQNAVGIFFEELSKSTGLVTFLAILTGIILSGLLV
                     AVTTVEVYDAFSVSFWDGIKEIFKAAWATYSSLFIGAFGDPTKIITAFQGGDGLAIRR
                     AINPFFESLVQATPYIFAGLSVALGFRVGLFNIGAEGQLFMGAMTSVFAGYAITGLPA
                     YIHIPVTLLAGALGGALWGFVPGFLKAKTGAHEVIITIMMNYIAFKLTDFLINGPMQD
                     PNEYTPKTPWIRESAHLTRFFGDPIRFHLGFFVALGVAFLIYWLLFKTTWGFELRTVG
                     SNPSGARYAGINTTKSTIIAMSLAGSLAGLAGANEVLGLNFRMVPSFSSGYGFDSIAL
                     ALLGKTHPFGVVLSALLFG"
     gene            complement(1073..2635)
                     /locus_tag="PLL88_08145"
     CDS             complement(1073..2635)
                     /locus_tag="PLL88_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01573.1"
                     /translation="MASLLELKGITKRFPGVLANDRIDLTLEKGEIHALLGENGAGKT
                     TLMNILYGLYSPDEGEIFVNGQKVTINGPTDAIRAGIGMVHQHFMLIPVFTVTENIML
                     GEEDVKMAGFLDRKKAAEKIEKISDTYKLNVNPNSYVKDLPVGTQQRVEIIKILFREA
                     EILIMDEPSAVLTPQEVDELFKIMRILVDQGKSIIFITHKLREVMECADRITVIRGGR
                     VVGSTTPKEADTKKLAAMMVGREVELKVKKTKAKPGNTVLHIENLRVLNAQEQFAVDG
                     VSFEVREGEVLGIAGVQGNGQTELVRALTGLLPPTEGKIEILGKETTKEKPRFISELG
                     SAHIPEDRQKDGLILAFPVSENLVLNSYFHKPFAKGIRLQFNEIAENGKKLVDNFDIR
                     TPSINTPVRNLSGGNQQKVIVAREFSRPIKLLIASQPTRGLDVGSIEYIHERLMKKRE
                     EGCAILLISTELDEVIQLSDRIAVMYRGKILDIVNAEDSSKEMLGLLMAGIKPSEEMK
                     QKAKKEEQIILT"
     gene            complement(2752..3834)
                     /locus_tag="PLL88_08150"
     CDS             complement(2752..3834)
                     /locus_tag="PLL88_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ01574.1"
                     /translation="MTKKITFVLSLLIIASLLLISCKAAAEETVVTQPAAEEPAEAAP
                     VMKFCQVTDVGGIDDKSFNTTAWAGMEDAAEDFGVEVKFLESQQQSDYEINLNAFIEE
                     GCDLIISVGFLLADATAAAATANPDVNFAIIDNGNMDLTNVRGNYSDIQGATFLAGYL
                     AAGMTQTGKVGTYVGILFPATQAFMDGYAMGVAYYNQVHGTAVEVLGWHMETQEGLEV
                     GNFESLDDGRAFGETLLDEGADIIMPVAGSVGAGTLAVMQERGTGLLIGVDLDWSVKY
                     PDQTDYILASTMKKINVFVYDSIEKIINGEFVGGEDFALTLENGGVGLVYGTAWADQV
                     PADLKAEIEALIPQIIDGSIATMPTR"
     gene            complement(3980..5224)
                     /locus_tag="PLL88_08155"
     CDS             complement(3980..5224)
                     /locus_tag="PLL88_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ01575.1"
                     /translation="MKYNIGKTFLLGLGFFGVSVIWSVYNAYVPIFLESRFALSAGFI
                     GVFMTLDNIAALFIQPAVGAWSDRLRTRIGRRMPFILIGAPIAALAFYVLPKAMALPV
                     FFFAASLLLLAMALWRTPVVALMPDITPSRYRSQANGIINFMGGIGAIIAFLFGATLY
                     EMNPSYPFWLGSLLVILAAVLVFLFIKEPKEFEHSKEKAPSLIESVKGIFKEKDRNTL
                     RILLAIFFWFIAYNGIEAFFTLYGKNYLGISESDAARLLGQLSTLFVLFSLPSGYIGA
                     AIGRKKTILIGIILMAACIASLFFLPASTLTTLLTTLPLLGKVPVIGVILMITGIAWA
                     LININSLPMVVDMTDANRIGTYTGLYYLFSTLAAILGPILYGFIIQFSAGMYNLLMII
                     SPVFLAGALISIIGVKRGEIKE"
     gene            complement(5221..6009)
                     /gene="trmD"
                     /locus_tag="PLL88_08160"
     CDS             complement(5221..6009)
                     /gene="trmD"
                     /locus_tag="PLL88_08160"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOJ01576.1"
                     /translation="MRFDVFTLFPEVFPAYLETSILKRAQENKLLEVRLHDIRDWASD
                     KHRTTDDTPYGGGGGMVMKPEPIFRAVDEILGIPPGCPLILLTPQGETFTQQKASAYS
                     KLPQLAMICGRYEGFDERIRQHLATQEVSIGDYVLSGGELPALVVIEAVTRLIPGALG
                     DPGGASDDSFSSGLLEYPQYTRPPIFQGWDVPEVLQAGDHGAIERWRRNQALLRTLQK
                     RPDLLLGVELSKADFDFLAQAVQGSEIALPDPIIKLIQNRKERS"
     gene            6147..7451
                     /locus_tag="PLL88_08165"
     CDS             6147..7451
                     /locus_tag="PLL88_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ01577.1"
                     /translation="MRRKNRRSNPLRILILIMVIALLVYIDQRVEPFSPYIFLPTPTP
                     TLSAATFITEAQSYAVEGKYTQALAAYAKAIKADPQNPSNYIASAMINVYRGDYETAL
                     DDASNALLLKPDNSQAYAIMGIAQGLIGDYLDSESSLTKAVEYDPSNYFAYAYDAVVK
                     AQKILEGSAAQGDLDLAIEASRKAEAISPSALETHWARGAVLELTANYEDAVAEYEAA
                     IAVNSNVSEIHISLGRTYVSMLEYTKAVEEYTKANALNPTDPIPDALIGRTYIRTGEY
                     SKAAQYYQQAISDDPSNAYYWGNLGYAYYKNYQFGDAILALQLAIRGGLTTNGVVVEG
                     LPLSTDVVTFYSAYGLALMQLGYCENNAVPIAQAIIQTMADDADAVWNAEYILEQCYQ
                     KMNNAQLLKLPTATMIPTWTPQPTATPTPLPYQVAVPTATGD"
     gene            7453..8775
                     /locus_tag="PLL88_08170"
     CDS             7453..8775
                     /locus_tag="PLL88_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ01578.1"
                     /translation="MNLPHRDSLFRKKKHRSNPYVIALLIAMIIYLLLINRDQSLGRI
                     TPLFMATPTATRTVDSFVEEAEVHFVAGDLAKALEAFQNAAVVNPNNVDLWVELARIQ
                     VYSSSLLISSEDKYNRLNEALESATRALEIDSDNSTAHAVKAFALDWLASSALMGDQS
                     EQLLTQAEQEAVAALQLDGNNTLALAYYAEILIDQMKWTQAEQYIDQALQQDDSLMDV
                     HRISGYLQESIGNYSAAITEYKQASEINPNLTFLYMSIGVNYRELGQYDYALEYFAKA
                     ADINEQLGVNDPLPYQSIAKTYVQQGYPLSASLNARKAVQLNPDNAFAYAFLGDVYHK
                     ARNYESEILAFQCGLEGCTAEESCEVRQCDSDTDTMIAVEGLPLTVDTKDYYAEYGAV
                     LAALHRESNGYCEKAMQILEMVRQAFSEDTILMGVVDESESICETYGY"
     gene            8909..9205
                     /gene="groES"
                     /locus_tag="PLL88_08175"
     CDS             8909..9205
                     /gene="groES"
                     /locus_tag="PLL88_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOJ01579.1"
                     /translation="MALNIKPLGSRLVVEPIEREDVTASGIVLPETAKEKPQKGIVLS
                     VGLGDRDEKGERIPLDVKENDTILFAKYSGTEIKIDDKKLLILKESDVLAIVKE"
     gene            9236..10870
                     /gene="groL"
                     /locus_tag="PLL88_08180"
     CDS             9236..10870
                     /gene="groL"
                     /locus_tag="PLL88_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561045.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOJ01580.1"
                     /translation="MAAKQLVFGENARNKLRSGIDVVAEAVATTLGPKGRNVALDRKF
                     GSPTITHDGVTVAKEIELEDPFENMGAQLLKEAATKTNDIAGDGTTTSTVLAHAMVAE
                     GLKTLAAGANPMLLKHGIEAASKKVADAIKAQAIDISTKEEIANVATISAQDRQIGTL
                     IADVMDKVGKDGVITVEESKGLEFEQEYVEGMQFDRGYISAYFVTDAEHMESVIENPY
                     ILIYDKKISAAQDLLPLLEKMVQVGKRDLVIIAEDVDGEALATLVLNKLRGTLNVLAV
                     KAPGFGDRRKAMLQDIAILTGASVISEETGRKLDTTTVQDLGTAEKVQSDKDNTTIIG
                     GKGDSKEIKGRIDQIRVEIDKTTSDYDREKLQERLAKLSGGVAIIRVGAATETELKEK
                     KHRVEDALSATRAAVEEGIVPGGGVALLNAVEALVDLKMDNEDEQTGVNIVRKALTVP
                     MKRIAENAGFDGPVVIENVHQAQSKDKSKKIGFDILSEKYVDMVKEGVIDPAKVTRGA
                     LENATSIAAMILTTEALITDIPEKEKPMAQPPMPEY"
     gene            10932..>11502
                     /locus_tag="PLL88_08185"
     CDS             10932..>11502
                     /locus_tag="PLL88_08185"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOJ01581.1"
                     /translation="MKKNSVSLLSLSIILLISLSACGQKGQEQPAATAAAATPTEEPM
                     AVRVNGSGITIEEFQNEQLRLKDAQAITGEVLSEEEQVDLILSELVGQVLLAQSANSE
                     GYFVDEATLQAHMDELIAQVGGSEQFMAWLQTNHYDESSFRQTLSRQLAAAWMRDKII
                     ASVPTSTEQVHARQILVGDRATADSLYQQL"
BASE COUNT         2971 a         2792 c         2901 g         2838 t
ORIGIN      
        1 aaaccgaaca ggagcgcgga gagaacgacg ccgaaaggat gcgtcttccc caatagtgca
       61 agggcaatgc tgtcaaaccc gtagcctgat gaaaaggacg gcaccattct gaagttcaac
      121 cccagcactt cgtttgcacc cgccaatccg gctaacgagc cggctaacga cattgcaatg
      181 atggtggatt ttgttgtgtt gatgcctgca tagcgagctc cgcttgggtt ggaaccaacc
      241 gttcgcagtt caaagcccca ggtggttttg aataacaacc aatagatcaa gaaagccacc
      301 cctaaagcga caaaaaatcc aagatggaat cgaatggggt cgccaaaaaa gcgggtaagg
      361 tgtgcgcttt cccgaatcca gggtgtcttg ggagtgtact cattggggtc ttgcatggga
      421 ccattgatca ggaagtcggt aagtttaaaa gcgatatagt tcatcataat cgttatgatc
      481 acttcatggg cgccggtctt agcttttaaa aatcctggaa cgaatcccca taatgcgcct
      541 cccagcgcgc ccgccaggag ggtaaccggg atgtggatgt aggcgggcaa tccggtgatg
      601 gcataccctg caaaaacgct ggtcatcgcc cccatgaaga gctgcccttc tgcgccgata
      661 ttgaacaagc ccacacgaaa gcccagcgca acagatagtc ctgcgaagat atagggtgtg
      721 gcttgcacca ggctttcaaa aaatggattg atcgcccggc gaatggctag tccgtcgccc
      781 ccctgaaatg ctgtaatgat cttggtcgga tcaccaaatg caccaatgaa cagggaagag
      841 taggttgccc aggcggcttt gaagatctcc ttgatcccat cccagaagga aacagaaaaa
      901 gcatcgtaga cttcgaccgt ggtgactgcg accagcagac cgctgagaat gatgccggtc
      961 aggatcgcca gaaaagtgac cagacctgtg cttttcgata attcctcgaa gaaaatccca
     1021 actgcgttct gttttttctt gttttgtgtt tgtttttcca tacctgcgtt cctcatgtca
     1081 agatgatctg ttcttctttt ttagccttct gtttcatctc ttcacttggt ttaataccag
     1141 ccatgagcaa accaagcatc tctttcgagg aatcttctgc gtttacgatg tcgagaattt
     1201 ttccgcggta catcacagct atgcgatctg aaagctggat gacctcatcc aattccgtgg
     1261 agatcagcag gatggcgcag ccttcctccc tttttttcat tagacgctca tgaatgtact
     1321 cgatcgaacc aacgtccaaa ccgcgggttg gttgtgaggc gatcagcaac ttgatcggtc
     1381 gtgaaaactc acgagccacg atcacttttt gctggttgcc gcctgaaagg ttccgtactg
     1441 gggtgttgat gcttggagtg cgaatgtcaa aattgtctac cagttttttc ccattttctg
     1501 caatttcatt gaactgcagg cgaatgcctt ttgcaaatgg tttatgaaag taagaattga
     1561 gcaccaaatt ttcggacacc ggaaaagcaa ggatcagccc gtctttttgg cgatcttcag
     1621 gaatatgcgc tgatcccaat tccgaaataa aacggggttt ctctttggtc gtttctttac
     1681 ccagtatctc gatcttccct tcggttggcg gtagcaaccc tgtcagggca cgcacgagtt
     1741 cagtttgacc attgccttgc acgcctgcaa ttccgagcac ttctccttct cgaacttcaa
     1801 aagacactcc atcgactgca aattgctctt gggcattcag tacgcgcagg ttctctatat
     1861 gcagcaccgt gttccctggt ttggctttgg ttttctttac cttcagctca acctcacgcc
     1921 ctaccatcat ggcagccaac ttcttcgtat cggcttcttt gggcgtggtt gaacccacca
     1981 ccctgccacc gcggatgacc gtgatacggt cagcgcattc catcacttca cgtagtttgt
     2041 gggtgatgaa aatgattgat tttccctggt caaccaaaat gcgcatgatc ttgaacaact
     2101 cgtctacttc ctgaggggtc aacaccgccg aaggttcatc catgatgaga atttccgctt
     2161 cacggaaaag gattttgatg atctccacgc gctgttgcgt tccgaccggc agatctttca
     2221 cgtaagagtt gggattcaca ttcaacttat aggtgtcgga tatcttttcg atcttctcag
     2281 cggctttttt tcgatccaga aatccagcca ttttgacatc ttcttcaccc agcatgatat
     2341 tctcggtgac tgtgaacaca gggatgagca tgaaatgttg gtgaaccatg ccaattccgg
     2401 ctcttatggc atctgttggt ccattgattg tgaccttttg accattgaca aagatctctc
     2461 cttcatctgg agaatacagc ccatacagaa tattcatcag ggtggtcttc cctgcgccgt
     2521 tttcccctag cagggcatgg atctcgcctt tttcaagcgt gaggtcaatg cggtcgtttg
     2581 ccagcacacc ggggaaacgt ttggtgattc cttttagttc aagtaatgac gccatttttc
     2641 cctcgtctga gtttaattgt ccaacaattg cgtgaattgt accatattgt gtttaacaaa
     2701 aagagccaag aaaaaaacct tggctctttt tgtttgtgtc gctctcgtta attaacgagt
     2761 gggcattgtt gcgatgctcc catcgatgat ctgtgggatc aaagcttcga tctcggcttt
     2821 gagatcggct ggaacctggt ctgcccaggc agtgccatag acaaggccca caccaccatt
     2881 ttcgagggtc agggcgaaat cctcaccacc aacgaattca ccattgatga ttttctcaat
     2941 gctgtcatag acgaacacat tgatcttctt catggtgctg gcgaggatgt aatctgtctg
     3001 gtcaggatac ttcactgacc aatccaaatc cacgccgatc aagagaccgg tgccgcgttc
     3061 ctgcatgact gccagggttc ccgcgccaac agatccagcg acgggcatga tgatatcggc
     3121 gccttcatca agcagggttt cgccaaaggc gcgcccgtca tcgaggcttt caaagttacc
     3181 gacttccaga ccttcttggg tttccatgtg ccaaccgaga acttcaacgg ctgttccatg
     3241 aacctggttg taataggcaa cgcccatggc ataaccatcc atgaaggctt gggtagcggg
     3301 gaagaggatg ccgacatagg tgccgacttt accggtctga gtcatcccgg cagccagata
     3361 gccagccagg aaagtggctc cttgaatatc ggagtagtta ccgcgaacgt tggtaagatc
     3421 catgtttccg ttgtcgataa tggcgaagtt gacatccggg ttggcggttg cggcggctgc
     3481 ggttgcatca gccaacaaga aaccaacgga gatgatcaga tcacagcctt cttcgataaa
     3541 cgcattcagg ttgatttcat agtcagactg ttgctgagat tcgaggaatt tgacttcaac
     3601 tccgaaatcc tccgctgcat cttccatgcc tgcccaggcg gttgtgttga atgatttgtc
     3661 atcaataccg ccgacatcgg tgacctggca gaatttcatg acaggtgcag cctctgctgg
     3721 ttcttcagca gcaggttgtg tgactactgt ttcttctgcg gcagctttac aactgatgag
     3781 cagaagagaa gcaataatca aaagacttag gacgaacgtg atttttttgg tcattatctc
     3841 tcctcctaga gaatttgaat tcgagagatg ctcttcgtct ctctttgaaa attaagtata
     3901 tcgtgtttaa aaatcaaaat caagttttcg acgatatgct ttccgtcctg tcattttgat
     3961 gacatcgatg gctctatttt tattctttta tttcacctct ttttactcca atgatgctga
     4021 tcagtgcccc tgccaggaag accgggctga tgatcatcag caggttgtac ataccggcgc
     4081 tgaattggat gatgaaccca tacaggatgg gacccagaat ggcagccagc gtagagaaaa
     4141 ggtaatacaa cccggtatag gtgccaatgc ggtttgcatc ggtcatatcc acgaccatgg
     4201 gtagggaatt gatgttgatc agcgcccagg cgatgcctgt gatcatcagg atcactccga
     4261 tgaccggaac tttacccaat agtggcaggg tggtaagcag ggtggttagg gtgctggctg
     4321 gcagaaagaa aagcgacgcg atgcaggcag ccatcaggat gatgccgatc aagatcgttt
     4381 tctttcgccc gatggcggca ccgatgtacc ctgaggggag ggaaaacagc acaaataagg
     4441 tggaaagctg tcctaacaaa cgtgcggcat ccgattcgct gattcccaga taatttttac
     4501 cgtaaagggt aaagaacgct tcaatgccat tgtaggcgat gaaccaaaag aagatcgcca
     4561 gcaggatgcg caaggtgttg cggtcttttt ctttaaaaat gcctttgacc gattcgatca
     4621 gtgatggcgc tttctctttg gaatgttcga actccttcgg ttctttgatg aatagaaata
     4681 ccagtactgc ggcgaggatt accaacagag atcccaacca gaaggggtag gatggattca
     4741 tttcatacaa agtggcgcca aacaaaaaag cgatgattgc cccaataccg cccatgaagt
     4801 taatgatccc attggcttgc gaacgatatc tggagggggt aatatcgggc atcaacgcta
     4861 ctaccggggt tcgccagagc gccatggcta gcaaaagcaa gctggcggca aagaagaaaa
     4921 ccggcagcgc catggctttg ggcagcacgt agaaagccaa cgctgcaatc ggcgcaccga
     4981 tcaggatgaa tggcattcgt cgcccgatgc gtgtgcgcag gcggtcagac caggctccca
     5041 cagcgggttg gatgaacagg gctgcgatgt tgtcgagggt catgaaaacg ccaatgaatc
     5101 ctgcggagag agcgaacctg gattcaagga agatgggcac ataggcgttg tagacgctcc
     5161 agattacgct gactccaaag aaacctaaac caagcaaaaa tgtctttcca atgttatatt
     5221 tcatgatctt tcctttctgt tttgtatgag tttaattata gggtcaggca gcgcaatttc
     5281 tgacccctgt actgcctgcg cgagaaaatc aaagtctgct ttggaaagct caacccccaa
     5341 taaaaggtcg ggtcgttttt gcagggtccg cagcaaagcc tggttgcgtc tccaacgttc
     5401 gattgcgccg tggtcgcccg cttgcagcac ttctggcaca tcccatccct gaaagatcgg
     5461 cggacgggtg tactggggat attccagcaa gccggaacta aaggaatcat cagaagcgcc
     5521 cccaggatct cccagtgcgc cggggataag acgcgtcacc gcttcgatca cgaccaacgc
     5581 tggcagttcg ccgccgctga gaacataatc accaatggac acttcctgtg tggcaaggtg
     5641 ttggcgaatg cgctcgtcaa aaccttcata tcttccacag atcatagcca gctgtggcaa
     5701 tttgctgtaa gcggacgctt tttgctgggt gaaggtttcg ccctgcggtg taagtaggat
     5761 gagcggacag cccggcggta tgcccaatat ttcgtccacc gccctaaaga tcggttccgg
     5821 tttcatcacc atcccgccac cccccccata gggggtatca tcggtggtgc ggtgtttatc
     5881 gctggcccaa tctcggatgt cgtgcaggcg gacttcgagg agcttgtttt cttgcgcgcg
     5941 ctttaagatg ctcgtttcaa gatatgccgg gaagacttcc gggaagaggg tgaaaacgtc
     6001 aaagcgcatg atttcattct agcgtgaacc aaaacgattt gcaagaaaac agatatcctg
     6061 gtatttgacg ctttttccct ttcttgacgc attttttaac atgatgataa gattgccgta
     6121 tgtatataaa aggaagaaat tggaatatgc ggcggaagaa ccgccgttcg aatcccttgc
     6181 gcattctcat tctgatcatg gtcatcgctc tgttggtgta catcgaccag agggtggagc
     6241 cgttctctcc atatattttt ctacctacac ccactccaac gttgtcggct gccacgttta
     6301 tcacagaagc gcagagttat gctgttgaag gcaagtacac acaagctttg gcggcatatg
     6361 cgaaagccat taaagcagat ccacaaaatc cttctaacta catcgcttca gccatgatca
     6421 atgtgtatcg gggtgattat gagacggcgc tggatgatgc cagcaatgcc ttattattaa
     6481 aacccgataa cagtcaggct tatgccatca tgggtatcgc tcaagggttg ataggagatt
     6541 atttagactc ggagtcaagc ctcacaaagg cagtagaata tgacccctct aactactttg
     6601 cttatgcgta tgacgcggtc gtgaaagccc aaaagatatt ggaaggctca gcagcccagg
     6661 gagatctgga tctggcgatt gaagcttcta ggaaagcgga agcgatttcc ccttcggcac
     6721 tggaaacaca ctgggctaga ggtgccgtgc tggaactcac tgcaaactat gaagacgcgg
     6781 tagctgaata cgaagcggct attgcggtta attccaatgt gtcggaaatc cacatttctc
     6841 ttggacgcac ctatgtcagc atgctcgaat acaccaaagc tgtggaagaa tataccaaag
     6901 ccaacgcgct caacccgacc gacccgattc ctgatgcgtt gatcggtcgc acctatatac
     6961 gcaccgggga atatagcaaa gctgcccagt attaccagca agccatttcc gatgatcctt
     7021 ccaatgccta ctactggggc aacctgggat atgcctatta taagaactac cagtttggcg
     7081 atgcgatcct ggcgctgcag cttgcgatcc gtggggggct gaccacgaat ggtgtggtcg
     7141 tcgaagggtt acccctttcg actgatgtgg taacgtttta ctcggcttac ggtttggctt
     7201 tgatgcaact tgggtattgc gagaataatg ccgtcccgat tgcccaggct atcattcaaa
     7261 ccatggcgga tgatgcagat gctgtgtgga acgcagaata cattcttgaa cagtgttatc
     7321 aaaaaatgaa caacgcacag ttgttgaaac tgcccacagc caccatgatc cccacctgga
     7381 ccccgcaacc cactgctacc cccaccccct tgccctatca ggtagccgtc cccaccgcta
     7441 cgggagacta gcatgaattt accgcatcgg gattcgttgt ttcgcaagaa gaaacatcgc
     7501 tcgaatcctt acgtgattgc acttctcata gccatgatca tttatttgtt gttgatcaat
     7561 cgcgatcaat ctctagggcg tatcacgccg ttgttcatgg ctacccccac cgccacgcgc
     7621 accgtagatt ctttcgtcga agaagcggag gtgcatttcg tggcggggga tctggctaaa
     7681 gctttggaag ctttccaaaa tgcagcggtg gtcaatccga acaatgttga tctttgggtt
     7741 gaactggcta gaattcaggt ctattcctcc agcttgctca tctccagtga agataaatat
     7801 aaccgcttga acgaggcttt agaatctgcc actagagcgt tggagatcga ctctgataac
     7861 agcacagccc atgcggtaaa agcctttgcg ttggactggt tggcaagttc ggcgctgatg
     7921 ggagatcagt cggaacagct tttaacccag gcagaacagg aagccgtggc ggcgttgcag
     7981 ttggatggca acaacacttt ggctttggct tattacgccg aaatcctgat tgaccagatg
     8041 aagtggacgc aggcggaaca atatattgac caggcgcttc aacaggatga ttctttgatg
     8101 gacgtacacc gcatcagcgg gtatctgcag gaatccatcg gaaattacag cgcagccatc
     8161 acggaataca agcaggcttc agagatcaac cccaacctta cgtttctcta tatgtccatt
     8221 ggagtgaact atcgcgaact gggtcagtac gattatgctc tggaatattt tgcgaaggcg
     8281 gctgacatca acgaacaact gggcgttaat gacccgctcc cttatcaatc gatcgcaaaa
     8341 acatacgtgc agcaggggta tcctctctct gccagtttga atgcgcgtaa ggcagtgcag
     8401 ctcaaccctg ataatgcctt tgcctatgcg tttttgggcg acgtctatca taaagcgcgc
     8461 aactatgaaa gtgagatcct ggcgtttcaa tgcgggttgg agggctgcac tgcagaagaa
     8521 agctgtgagg tgcgccagtg tgattctgac accgatacga tgattgccgt cgaagggctg
     8581 cccctgacgg ttgacacgaa agattattac gccgaatatg gggcggtgtt ggcagccttg
     8641 catcgtgaat cgaatggcta ctgtgaaaaa gctatgcaga tattggaaat ggtacggcag
     8701 gctttctcag aggatacgat actcatgggg gtggtggatg aatcggagtc catttgcgaa
     8761 acgtatggct attaaatgtt aaaaaaatat taattttttt ccatttactt gacttgctgt
     8821 taaaagacgg ataagattga aattagcact ctctgaaaaa gagtgctaaa ataatgactc
     8881 gataaaatac ttatcaggag gatcatatat ggcactcaat atcaaacccc ttggaagccg
     8941 attggtcgtt gagccgatcg aacgggaaga tgtaactgcc tcaggcatcg tgctgcctga
     9001 aaccgccaaa gaaaaacccc aaaaaggaat cgtcctttcc gtaggacttg gcgatcgtga
     9061 cgaaaaaggt gagcgcatcc cgctggatgt caaggaaaac gacaccattt tgttcgcaaa
     9121 atattcaggc actgagatca agatcgacga caagaagctc ttgatcttaa aagagagcga
     9181 tgttctcgcg attgtcaaag aataattcaa ttaatctcga aagaaggaga tacatatggc
     9241 agcaaaacaa cttgtttttg gagaaaatgc gcgaaataaa ttgcgcagtg gtatcgatgt
     9301 tgtggcagaa gcagttgcta ctacccttgg tccgaaaggg cgcaatgtag ccctggatcg
     9361 caaatttggt tccccgacca tcacccatga tggtgttacc gtagcaaaag aaatcgaatt
     9421 ggaagaccca tttgagaata tgggcgctca attattgaaa gaagccgcca ccaagaccaa
     9481 tgacatcgcc ggcgatggca ccaccacctc aaccgttctg gctcatgcca tggttgccga
     9541 aggactgaaa accctggctg ctggcgccaa cccaatgctg ttgaaacatg gcattgaagc
     9601 tgcatccaaa aaggttgcag atgccatcaa ggcacaggca attgatattt caacgaaaga
     9661 agagattgcg aatgtagcca ccatttcagc gcaagatcgc cagataggaa ccttgattgc
     9721 tgatgttatg gacaaggtcg gaaaagatgg tgtgatcacc gtcgaggagt caaaaggtct
     9781 tgaatttgag caggaatatg tagaaggtat gcaatttgac cgcggttaca tttctgccta
     9841 ttttgtgacc gatgctgagc acatggaatc agtcattgaa aacccctaca tcttgatcta
     9901 tgataaaaag atttccgccg cacaggatct tttgcccctc ctggagaaga tggtgcaggt
     9961 tggaaaacgt gacctggtca ttattgccga agacgttgat ggtgaagcgt tagccaccct
    10021 cgtgttgaac aaactgcgtg gcacccttaa cgtgctggct gtcaaagctc ctggcttcgg
    10081 cgatcgccga aaagccatgc tgcaggatat tgccattcta accggcgcct ctgtcatttc
    10141 tgaagagacc ggtcgcaagc tggatacgac caccgttcag gatcttggta ccgccgaaaa
    10201 agtgcagtct gacaaagaca acaccaccat catcggtggc aaaggcgatt ccaaggagat
    10261 caaaggtcgc attgaccaga tcagggttga aatcgacaag accaccagtg attatgatcg
    10321 ggaaaaactg caagaacggc ttgccaaact ttctggtggc gttgccatta ttcgtgttgg
    10381 tgctgcaacc gagaccgaac tgaaagagaa gaaacatcgc gtagaagatg ccctttccgc
    10441 tacccgtgct gcggttgaag aaggtatcgt gcccggtggt ggagtagccc tgttgaatgc
    10501 ggtagaagcc ctggttgacc tgaagatgga taacgaggat gaacagaccg gcgtcaatat
    10561 cgttcgcaaa gcgctcaccg ttcctatgaa acgcatcgct gagaatgccg gatttgatgg
    10621 accggttgtg atcgaaaacg ttcatcaggc tcaaagtaag gataagagca agaaaatcgg
    10681 tttcgacatc ctttctgaga agtatgtgga tatggtcaaa gaaggtgtga tcgatcccgc
    10741 caaagtgacc cgcggcgctt tggaaaatgc gacttccatc gcagcgatga tcctcacgac
    10801 cgaggctctc atcaccgata ttcctgaaaa ggaaaaaccg atggcgcaac cgcccatgcc
    10861 cgaatactag catgaacagc cgtaggataa ccccctacgg cttttttatt tccgtttata
    10921 atcagcgcta tatgaaaaaa aatagcgtct cactgctttc tctgagcatc atcctcctaa
    10981 tatcgttatc tgcctgtggg caaaaagggc aggagcaacc agccgccacc gcggcggcag
    11041 ccacgcccac cgaagaaccc atggcggtgc gcgtaaacgg cagcggtatc acgatagaag
    11101 aatttcagaa tgagcaattg cgtctcaaag atgcgcaagc gatcacaggg gaagtattga
    11161 gtgaagagga acaggtcgat ctgatccttt cagaactggt ggggcaggtg ttgctggcgc
    11221 aatcggcaaa cagtgaggga tattttgtgg atgaagccac cttgcaagct catatggatg
    11281 agttgatcgc acaggtgggg gggtcagagc agttcatggc atggctgcag accaaccatt
    11341 atgatgagag cagtttccgc caaacgttga gcagacagtt ggctgccgcc tggatgcgcg
    11401 acaagatcat cgccagtgtg cctacctcta cagaacaggt gcatgctcgt cagatcctgg
    11461 ttggtgatcg cgccacggct gattccctgt atcagcagtt gc
//
LOCUS       DAOSDS010000068        11434 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2175_length_11434_cov_4.913525, whole genome shotgun sequence.
ACCESSION   DAOSDS010000068 DAOSDS010000000
VERSION     DAOSDS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11434
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2175_length_11434_cov_4.913525"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..1236)
                     /locus_tag="PLL88_08190"
     CDS             complement(13..1236)
                     /locus_tag="PLL88_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01582.1"
                     /translation="MNAVRFFKEAISSLLSNKMRTFLTMLGIVIGVAAVISMMAIGNG
                     AQESITSSIESMGTNLIFISQNNDASNPEALTLSDATAISEAATQYIAAVAPSVQNSM
                     SVSYSGNSITAQIYGVTPEYESVRNTAVSEGSFITQSQVDERSTVAVIGVDIAEELFN
                     TTQNVVGNKIRMGSYVYTVIGLLEKQGGSFSGSSDNQVFIPITTAQARIISRTNAHDE
                     ISMISISATSSDDMESAIEEVTKVMQNQHGILGGEGNDFTVMSQESITEAASSITGVL
                     TIFLGGIAAISLLVGGIGIMNIMLVTVVERTREIGLRKAVGARKRDILTQFLIEALVI
                     GLVGGILGVLVGAGISAVISKIAAFSETVLNPKVSFSSVLLSTLFSIGVGLVFGIYPA
                     NRAANLEPVEALRTE"
     gene            complement(1233..1910)
                     /locus_tag="PLL88_08195"
     CDS             complement(1233..1910)
                     /locus_tag="PLL88_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01583.1"
                     /translation="MKKNVVEAHDLVKTYQLGEVQVEALRGLSLHIRRGEIVAIMGPS
                     GSGKSTLMNILGCLDRPTSGQYILDSEDVSQLSDDQLADIRNRKIGFVFQSYNLLQRT
                     NALDNVMLPLRYKRDNANNHTEMAAAILERVGLKDRMNHKPFELSGGQQQRVAIARAL
                     INNPSIILADEPTGNLDSKVGHEIMQLLLELNKEDNVTLIIVTHDSGVAKITDRTIHI
                     IDGKVEK"
     gene            complement(1919..3451)
                     /locus_tag="PLL88_08200"
     CDS             complement(1919..3451)
                     /locus_tag="PLL88_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ01584.1"
                     /translation="MKIKTFFQTIAKKIVDYYGKNKKRSIILGSVALVLIALLSVFLT
                     IRRNANNNSETQAEIVTLTTGTFSQTIDVVGTVRAVPSATLTWQTSGIVSPLAVSVGD
                     SVKQGDILLTLDASSLPSSVLQAQSDLLNAQYELDKLMVANSALQEAALALDDAEYDY
                     AAKKQMRDFWNFNNTSDEKIDEARAAYQQSEEDLWNAQHAYEALSDLADDDPEKIAAA
                     EAVDAAQLTRDKAYRNINYLLGRTYDHTVETDFIEFDQAKATLEEARVTYEKYKDNSE
                     EIAAAEANVQALQNTVNSAYVIAPFDGVITDILADEGDIVSSGTQAIQLDDMSNLVID
                     IAVSEIDINEIEIGQSVNIIFDAIPNKTYEGSVSKVSLAGYESSGIVEFTVTISITNA
                     DEGVKPGFTAVATVMINEVENAVLVPNLAIQTMDGSSVVMVVDADNSITAIPVEVEAS
                     SATYSIVKDGALQEGDQVMVFVNSNTNASLFGMFGLGGGMMGGGEQPVGEPPQNTTRP
                     QQ"
     gene            3986..4906
                     /locus_tag="PLL88_08205"
     CDS             3986..4906
                     /locus_tag="PLL88_08205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOJ01585.1"
                     /translation="MILLNLFVAWLFSRKTLLLFCVFITVVEIAISLLSIQTDPFSFG
                     ILIVTIVFRSVILGVMGLFVNSMSELQNSQQEKLEQANIRLRKYAIYAEQLAQTRERN
                     RLARELHDTLAHTLSSVAVQLEAVKALFDSNHEEAKKRLEHSLVTTRDGLDETRRALK
                     DLRTSELENFGLCQSLRNLLQNGAARSSFQYTENLTEALNLLPDEISHAIYRTVQEAV
                     DNTVKHANAKKVDLQIDLSDKNLQLVYCDDGQGFSLEETDDVKHYGLQGMRERIDLLG
                     GALTIQSEPNQGTRIMIRMEWDNDKNIDLR"
     gene            4881..5522
                     /locus_tag="PLL88_08210"
     CDS             4881..5522
                     /locus_tag="PLL88_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ01586.1"
                     /translation="MIKILICDDQELVCEGLNAILGTSNLVEVQGMCYNGLEAIEFIE
                     KHPVDVVLMDLKMPILNGIQATKQIKEKFPNVHILVLTTYDADQWVLDAIRYGADGYL
                     LKDAPRERLLQAIEEVNSGRTPVDSKVAGKIFEQISKMPQKSPTTVGSNLTEREREVL
                     MLISHGKSNAEIAESLYLSEGTVRNYVSSILEKLEVTDRTQAAVIAIRSGLVD"
     gene            complement(5572..6687)
                     /locus_tag="PLL88_08215"
     CDS             complement(5572..6687)
                     /locus_tag="PLL88_08215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ01587.1"
                     /translation="MGKFKRYTLFTAILIMAMLSGSCAKSSSGTDVASEAISLQITQV
                     DTSQFPLVRVYVSAKDQAGNPVPVPVSDIELLEDGAPINAQAIQGMDEVTSFTSLLVM
                     DNSGSMNFSDKLETAKRAASSYIEQMRSADQAGIITFNTQVQTVQEITADKDALLAGI
                     AGVRARDDTAMWDALLSAVETLNPISGRKVIIVLTDGMDNQSSATPEQILSTIGESGL
                     SISTIGFGTKPEEGVAPDEYEGIDEQTLTNLAEQAGGIYGYVEDSEALLDLYATIRES
                     LQSEVVISYITPQTLRDGVKRALSVRLSGAWESHVGGSSSAYNPGGLIPEVPNPNNWL
                     LFLILLGALMILLVVPLFLTAMAAKKKKKKIRIKLLD"
     gene            complement(6692..7549)
                     /locus_tag="PLL88_08220"
     CDS             complement(6692..7549)
                     /locus_tag="PLL88_08220"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOJ01588.1"
                     /translation="MKRNMRFYTLEITGAIGGLLGWQLSNLLGLSFTSNFFVSEVIIG
                     ALIGGLIGLFIGIGEGLLAQSWGVGLRKGAVALLLGAVGGCIALPLAESAFLAIGGEV
                     WGRPFGWALFGLLVGFATSITGGSQLWKGGLGGFIGGLVGGALLETARALLSDPALGK
                     AAGLMLLGFSTGVFTALISFALSRTWLEVTSGKITGMEFILDKFLRSHGPSATIGSSP
                     LKAEVAIPDPGIDPQHAILEGHDTYFTLKDLSNSGTFIDGKKVDVAKLKNNQHIRMGK
                     TEMIYHEKR"
     gene            complement(7546..8175)
                     /locus_tag="PLL88_08225"
     CDS             complement(7546..8175)
                     /locus_tag="PLL88_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HOJ01589.1"
                     /translation="MATKNSRIPTRQALEWIPRQKPELLLDPLVKVFITQNAYIRICA
                     HAGSDLENEVGGWMAGKYCTDTVANEQFIVIDTILAAPYAEHGAAHLTFTSNTQIALY
                     DFLERYYPDKVLVGWYHTHPKMGIFFSSWDAWLHKNFFSKPWQVALVVEPYTAAGGFF
                     IRQKNGELDTQIYFGFHELLNREKKSKVFWKNLEPRRNLNTLETEVLIV"
     gene            complement(8249..8515)
                     /locus_tag="PLL88_08230"
     CDS             complement(8249..8515)
                     /locus_tag="PLL88_08230"
                     /inference="COORDINATES: protein motif:HMM:NF020396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HOJ01590.1"
                     /translation="MSDKNVTLVLPSGGSRNAEVPDDVEVKDLLPELATTLELPTVGP
                     DGRPVSYRLDSKALGRELKEDETLTSAGVPDNDRLMITADITAG"
     gene            complement(8530..9381)
                     /locus_tag="PLL88_08235"
     CDS             complement(8530..9381)
                     /locus_tag="PLL88_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HOJ01591.1"
                     /translation="MADISVTLVLPNGGARKAEMPDDVTVRDLLTELTSLLGLPTVGP
                     DGRPLGYRMDSKALGRELRESETLGQAHIPHDDRLMITTDITAGAVSVSSSPRMRRLK
                     KDYELIQEINARSDLIQVKAEKTPGNLPPEKYIITFSCKGIIGIDRKGNPKFGDKHQV
                     EIYLHNQYPQRWPGMKWLTPIWHPNINHANGSVCIDAAWWSASRSLDRLVIMLGEMVQ
                     YKNYHDDPKKPPFPWDMEAAQWCQKYRKAHPEAFPVDKRELLRPERIKIVKKAQPAKI
                     KPRIKLK"
     gene            complement(9403..10254)
                     /locus_tag="PLL88_08240"
     CDS             complement(9403..10254)
                     /locus_tag="PLL88_08240"
                     /inference="COORDINATES: protein motif:HMM:NF025802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HOJ01592.1"
                     /translation="MKKINFFHGFLIFLLILSVGFGSGYLILYYLTPQTQISQLITRA
                     LILLVVGIGVGLVCRLTVPVRMWFLKICFGSFGSLLSIAALDWYNPTAFALLRDRTLQ
                     IAWWSIGEYTQIGLVLIITAFIALIGKKKPVSVVHKETSKLESQKNVAKRKPASLPVK
                     SVAIKKKRKPIIDLKALTKRKVIRKTGTQKKVITNSSPKQSSQALRVKSTKKSSAKRA
                     PTTRMWSRKKVKLTGTEDHHCPYCLEEVHKNDPRGVMICPDCGTWHHRDCWEITGSCQ
                     IAHKHEL"
     gene            complement(10251..>11434)
                     /locus_tag="PLL88_08245"
     CDS             complement(10251..>11434)
                     /locus_tag="PLL88_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOJ01593.1"
                     /translation="LGNEVIKNLVLMGIGYLYIVDFDTIEMANLSRSVLFRESDKGRK
                     KAEVAAARAKEINPNVHVQYLHGDVTTDIGLGVFRRMDVIIGCLDNREARLAVNRFAY
                     WMNKSWVDGAIQEFFGLARVFVPGEGACFECTLTEQARRDLSIRYSCPLLARENILLG
                     KVPTTPTIASIIAAIQSQEALKLIHGKPVEAGKVIHFNGMTNEMHKTSYTPAEDCESH
                     WTYGKITELPLRADTTTLEDMLKIIHRDLGPQAVLELDQELILTLYCRNCQTKEDILQ
                     PISQVGFNHAHCPTCGMLRETEMTHIVTGEEPYINRTLFSLGVPKLHIMRAYNADQYA
                     FYELSGDLDNALHFKHFQKPVIKLDDVPVIKIATTEDEKTGAKPSTRSRIKLETDKTI
                     K"
BASE COUNT         2791 a         2770 c         2691 g         3182 t
ORIGIN      
        1 tttccatcgc ctctattctg ttcgtaacgc ctccaccggt tccagattgg ctgcacggtt
       61 ggcagggtaa attccaaaaa ccagaccgac accaatcgaa aacagcgttg aaagcaacac
      121 ggaagagaaa ctgaccttgg gattgagcac agtttcacta aacgctgcta ttttgctgat
      181 cactgctgaa atgcctgcgc ccactaacac acccaatatt ccccccacca agccaatgac
      241 caatgcttcg atcaggaact gtgtcaggat gtctcttttt ctggcaccga ctgctttgcg
      301 caagccgatc tcgcgggttc tttcaaccac ggttaccagc atgatgttca taatgccgat
      361 accgccgacc agcagcgaaa tggcagcaat gccgcccaga aagatggtca agacgccggt
      421 gatggagctg gctgcttcag taatgctctc ctgcgacatg accgtaaagt catttccttc
      481 accacctaaa ataccatgct gattttgcat gaccttggtc acttcttcga ttgcgctttc
      541 catatcgtct gaactggtgg ctgaaatgga gatcatgctg atttcatcgt gcgcgttggt
      601 gcgcgaaatg atccgcgcct gagccgtggt aattgggatg aagacctgat tatcagagga
      661 acccgaaaag gacccccctt gtttttccag caatccgatc accgtgtaaa cataactgcc
      721 catgcggatc ttatttccca ccacattttg tgtcgtgttg aataattctt cggcaatatc
      781 cactccgatg acggcaacgg ttgaccgttc atccacttga ctttgcgtga taaatgatcc
      841 ttcactgacc gcagtgttgc gcacagattc atattcggga gtaactccgt aaatctgtgc
      901 ggtgatggag ttgcctgaat acgataccga catgctgttt tgaaccgaag gcgcaactgc
      961 cgcaatgtat tgcgttgctg cttcgctgat cgcagtggca tcggaaagcg taagcgcttc
     1021 gggattgctg gcgtcattgt tctgcgaaat aaagatcagg tttgtgccca ttgattcgat
     1081 tgaactggtg atcgattctt gggcgccatt gccaattgcc atcatggata tcactgcggc
     1141 aactccaatg acgattccca gcatggtcag gaaagtccgc attttattcg aaagcaggct
     1201 gctgattgct tctttgaaaa acctcactgc attcattttt ccaccttccc atcaatgatg
     1261 tggatggttc tgtccgttat ttttgccact cccgaatcat gggttacgat gatcaatgtg
     1321 acgttatctt ctttatttaa ttcaagcagc aactgcatga tttcatgtcc aaccttggaa
     1381 tccaggtttc cggtgggctc atcggccaaa atgatcgagg ggttgttgat cagcgccctg
     1441 gcgatagcta cacgttgctg ttgaccgccg gaaagttcga agggcttgtg gttcatgcga
     1501 tcttttaatc ctacacgttc caggatggca gcagccattt ctgtatggtt attggcattg
     1561 tctcgtttat aacgcagcgg caacattaca ttatccagcg cattggtcct ttgcagcaga
     1621 ttgtagcttt gaaacacgaa gccgatcttt ctgtttcgaa tatctgccaa ctggtcatcg
     1681 gataactgag agacatcctc gctgtccaaa atatactgcc ctgaagtagg gcgatccagg
     1741 caacccagga tgttcattaa agtcgatttt cctgatcctg agggtcccat aatcgctact
     1801 atctcgcccc ttcttatgtg cagtgacaga ccacgcaggg cttctacctg tacttcgccc
     1861 agttgatatg ttttcaccag atcgtgggct tcgacaacat tctttttcat tgacacctct
     1921 attgttgcgg tcgggtcgta ttttgtggtg gttcgccaac gggttgttcg ccgccaccca
     1981 tcattccgcc acctagtccg aacatgccga aaagggaagc attggtgttg ctgttaacaa
     2041 aaaccatcac ctgatcgcct tcctgcaaag caccatcttt tacgatcgag taggtggcag
     2101 agcttgcttc cacttcaaca ggtatggcgg tgatgctgtt atctgcatct acgaccatga
     2161 ccacactcga tccatccata gtctggatcg ccagattggg aaccagaaca gcgttttcca
     2221 cttcgttgat cattacggta gccacggcag taaaaccggg cttaacacct tcatccgcgt
     2281 tcgtgatcga aatcgttacc gtgaattcca caataccgga tgattcgtat ccagccaagg
     2341 aaacctttga aacggatcct tcgtaggttt tatttggaat tgcatcgaag atgatgttga
     2401 cggactgccc aatctcgatt tcgttaatgt cgatttctga aacagcgata tcgataacca
     2461 gattactcat atcgtccaat tggatggctt gcgtaccact cgaaacaata tcaccttcgt
     2521 ccgccagaat atcggtgatc acaccgtcaa acggagcaat aacatacgct gaattcactg
     2581 tattctgaag cgcctgaaca ttggcttctg ctgcggcaat ttcctcagaa ttatccttgt
     2641 atttttcata cgtcacacga gcttcttcaa gtgtagcttt ggcttgatcg aattcaataa
     2701 aatctgtttc cacagtgtgg tcataggtgc gccccaaaag gtaattgata ttgcgatatg
     2761 ctttatcgcg cgtcaattgg gctgcgtcca ccgcttcagc cgcggcgatc ttttctggat
     2821 cgtcgtcagc cagatcgctc agtgcttcat aagcatgttg cgcattccaa agatcttctt
     2881 ccgattgttg ataggctgct ctggcttcat caattttttc atccgatgta ttattgaaat
     2941 tccagaaatc tcgcatttgt tttttcgctg cataatcgta ttcggcatca tccagtgcaa
     3001 gagctgcctc ctgtaaagct gaattagcaa ccattaattt atccaattcg tattgcgcgt
     3061 tcagaaggtc tgattgtgcc tgcaaaacag aagaaggcag cgagctggca tccagggtca
     3121 gcaatatatc accttgtttt acgctgtcac ctacgctgac cgctaacgga ctgacaatcc
     3181 cactggtctg ccaggttaaa gtagcagaag gaaccgcacg cactgttcca actacatcaa
     3241 tggtttgaga aaatgttcct gttgttaacg tcacgatctc tgcttgggtt tcggaattat
     3301 tgtttgcatt tctacgaatg gtaaggaaaa cactcaatag cgcaataaga accaacgcga
     3361 cactacccag gatgatagat ctttttttat tttttccgta gtagtccacg atctttttgg
     3421 ctatggtttg aaaaaaagtt tttatcttca ttcatgaatc tccatcttgc tttgaatttt
     3481 gttttgaata tatacaggtc cggattgaat ctggagtgca agggatcacg tctcatcatt
     3541 atttttttac agaaagaggg tgattccagt aatggcggca ggttaaaaaa atcacggaca
     3601 gaaataaatt ggactgtgtt aagatgaaga catggatgaa gcggaaatca acgcttggca
     3661 agtgaaatta ctgcgtattt atgttctgtt ccagttctgt atttatctct ccagtcagat
     3721 cattcgggtt ctatttttat cagatatagc gatcagtgat ttcaattggg tcagatttgt
     3781 gcctgctgtt atgctgttcg tggtctttat tcttttatgc cttcatagat ttcaaaggaa
     3841 tatcacaaaa gcaaggtttt tatccttgat cgttatatta tctgtaacaa cgatcgtatc
     3901 gagattttta acccctgatt tctcagttaa ccgggcttct ctcatgccag gggtcctcaa
     3961 ttttaatctt gatcagatat ttttcttgat cctgctgaac ttatttgtcg cctggttgtt
     4021 ctcacgaaaa accctgctcc tgttttgtgt ctttattacc gtcgtagaaa tagccatctc
     4081 cctgctctcc atccaaaccg atccgttcag ttttggcatt ttgatcgtta ccattgtttt
     4141 tcgcagcgtg attttggggg tcatggggct tttcgtcaac agcatgagtg aattacagaa
     4201 ttctcagcag gaaaagctgg aacaggcaaa tatccgattg agaaagtatg cgatctatgc
     4261 agaacaatta gcgcaaacac gtgagagaaa tcggttggcg cgtgaactgc acgatacgct
     4321 tgctcacacc ctaagcagtg tggcagttca attagaagca gtaaaagctt tgtttgattc
     4381 caatcatgaa gaagcgaaaa agaggcttga gcatagtttg gttaccaccc gggatggatt
     4441 ggatgaaacc cgccgtgccc tgaaagactt gaggacttcg gaactggaaa attttggtct
     4501 ttgtcaatcc ctgcgaaatc tcctgcagaa tggggctgcc cgcagcagtt ttcaatatac
     4561 ggagaatctt accgaagccc tcaatctttt gccagatgag atcagccatg ccatctaccg
     4621 gacggttcaa gaggcggtcg ataacacagt aaaacatgcc aatgcaaaaa aagttgatct
     4681 tcagatcgat ctctccgata aaaatttgca acttgtctat tgtgacgacg ggcagggctt
     4741 ttcattagaa gagacagatg atgtgaaaca ttatgggctg cagggtatgc gcgaaaggat
     4801 cgacctgttg ggcggagccc ttacgatcca aagtgaaccc aatcagggca ccaggatcat
     4861 gatcagaatg gagtgggata atgataaaaa tattgatctg cgatgaccag gaacttgttt
     4921 gtgaggggtt gaatgctatc ctgggcacat ccaatctggt tgaagtgcag ggcatgtgct
     4981 acaacgggtt ggaagcgatc gagttcatcg agaaacatcc ggtggatgtg gttctcatgg
     5041 atttaaaaat gccaatcctt aatggtattc aggctaccaa acagatcaaa gagaaattcc
     5101 ccaatgtgca tattttggta ttgaccacct atgatgctga tcaatgggtt ctggatgcca
     5161 ttcgttatgg cgcagacggc tacctgctga aagacgctcc tcgcgaacga ctgttgcaag
     5221 ctattgaaga ggtcaattcc gggcgcactc ctgttgattc aaaggtagca ggcaagatct
     5281 ttgagcaaat ttcaaaaatg ccgcaaaagt ctcccaccac cgtcggatca aatttaacag
     5341 aacgtgagag ggaagtgtta atgctgatct cccacggaaa atccaatgca gagatcgcgg
     5401 agagtcttta tctctctgaa ggtacggtgc ggaactatgt cagctccatt ctagaaaaat
     5461 tagaagtgac tgatcgtacc caggcggctg tcattgcgat ccgcagtgga ttggtggatt
     5521 agaaaagaat ggttttcata aaaacagact cccatttcgg gagtctgttt tttagtccag
     5581 cagtttaatg cggattttct tctttttctt ctttgcagcc atagctgtca agaacagggg
     5641 aacgaccagt aagatcatta atgccccaag cagaataagg aacagcagcc aattgttggg
     5701 gttgggaacc tccgggatca gccctcccgg attgtaggcg gaggatgaac cacctacgtg
     5761 actttcccag gcacccgaca atcgaacgga aagggcgcgt ttgacgccat cgcgcaaggt
     5821 ctggggggtt atatagctga tgaccacttc actttgcagt gattcgcgaa tggtggcata
     5881 taggtctagc aatgcttcag aatcttccac gtatccataa attcccccgg cttgttcagc
     5941 cagattggtc aaggtttgtt cgtcgatccc ttcatattca tccggtgcga cgccttcttc
     6001 cggtttcgtc ccaaagccga tagtggaaat ggagagaccg ctttccccta tagtggaaag
     6061 gatctgttca ggggtagcgc tgctttgatt atccatgccg tcggtcagca caatgatgac
     6121 ttttcttccg ctgatcggat tcagcgtttc cactgcgctt aacagggcgt cccacatggc
     6181 agtgtcgtcg cgagcccgta ccccggcaat gccggctaac agggcatcct tatcggcagt
     6241 gatctcctgt accgtttgta cctgagtatt gaacgtgatg atccctgcct gatctgcaga
     6301 tcgcatctgt tcgatgtatg aggatgccgc tcgtttggca gtttccagtt tgtcggaaaa
     6361 gttcatgctg cccgaattat ccatgaccag cagactggtg aaagatgtga cttcatccat
     6421 accttgaatt gcctgggcgt tgatgggggc accatcttca aggagttcaa tatccgatac
     6481 cggaacgggc acgggattac cagcctggtc tttggcagat acatacacac gaacgagcgg
     6541 aaattggctg gtatctacct gggtgatctg caatgagatc gcttcagagg ctacgtcggt
     6601 tcctgacgag ctttttgcgc aactaccgct cagcattgcc atgatcaaga tggctgtaaa
     6661 gagggtgtat cttttgaatt tacccatagt attacctctt ttcatgatag atcatttccg
     6721 ttttccccat gcgaatatgc tgattgtttt tcagctttgc aacatccacc ttttttccat
     6781 cgatgaatgt gccgctgttg ctcagatctt tcagggtaaa atatgtgtca tgcccttcca
     6841 ggatagcgtg ttggggatcg atacctggat cagggatggc aacctcggct ttgagtgggc
     6901 tgctgccgat agtagcggag ggaccatggg atctcaggaa cttatccaaa ataaattcca
     6961 tgcctgtgat cttgccactg gtgacctcta gccaggtgcg agagagggca aaactgatca
     7021 gcgcggtgaa aacaccggtt gaaaagccca agagcatcaa accggcggct tttccaagcg
     7081 ctggatctga gagaagggct ctggcggttt ccagaagcgc gccacccacc agaccaccaa
     7141 tgaatccgcc cagcccgcct ttccagagct gcgatcctcc tgtgatagag gtggcgaatc
     7201 ccaccagcag cccaaagagc gcccagccga aaggacgtcc ccacacctca ccaccgatcg
     7261 cgagaaacgc actttcagcg agcggtaagg cgatacaacc tcccaccgcg ccaagcagca
     7321 aggcaaccgc tccttttcta agaccaaccc cccacgattg cgccaacaga ccttccccaa
     7381 tcccgatgaa caaccctatc agtccaccga tcaatgcccc gatgatcact tcgctgacaa
     7441 aaaagttgga tgtgaaggaa agtccaagca ggttgctcaa ctgccatccg agcaatccgc
     7501 cgatagcgcc tgtgatttcc agcgtataaa aacgcatgtt tcgtttcata caatcaatac
     7561 ctccgtttca agggtattta aattccgtct cggttccaga tttttccaaa aaaccttgct
     7621 tttcttctca cgattgagca gttcatggaa accgaaatag atctgcgtgt ccaactcgcc
     7681 atttttttgc cggataaaga atccaccggc tgcagtgtaa ggttctacga ccagcgcaac
     7741 ctgccagggt tttgaaaaaa aattcttatg caaccacgca tcccacgaag agaagaaaat
     7801 tcccatctta gggtgtgtgt gataccagcc taccagtact ttatcggggt aatagcgctc
     7861 tagaaagtca tacaaagcaa tttgcgtatt gctggtaaaa gtcaggtgag cggcgccatg
     7921 ttctgcatag ggagcagcga gaatggtatc gatcacgatg aactgctcgt tggcgacggt
     7981 gtctgtgcag tactttccag ccatccaacc acccacttca ttttccagat cgcttccggc
     8041 atgtgcgcaa atacgaatat aggcattttg tgtaatgaac accttcacta atggatccag
     8101 caaaagctcg ggcttttgcc ttggtatcca ctccaacgct tgacgtgtgg gaatgcgaga
     8161 atttttggtt gccatttcaa tttcctgttg gagctgaaga cggaattgtt tttccaaagg
     8221 atttctcgtt cttgaatgac tgaccgcttt aaccggcagt gatgtcagcg gtgatcatca
     8281 aacgatcatt atctggcacg ccggctgaag tgagggtttc gtcctctttc aattcgcgac
     8341 ccagagcttt gctgtcaagc cggtaactta cgggacgacc atccggaccg actgtgggta
     8401 attcaagtgt ggttgccagc tcaggcagca gatctttgac ctcaacgtca tccggcactt
     8461 ctgcattgcg cgatccacca gaaggtaaaa cgagggttac atttttatca gacatttttc
     8521 tctccttttt tatttcaact tgattcttgg ctttatcttg gcgggttgtg cttttttcac
     8581 gatcttgatg cgttcagggc gcaacaattc ccgcttgtct accgggaagg cctccggatg
     8641 cgctttccgg tatttctgac accattgtgc tgcttccata tcccatggaa acggcggttt
     8701 ttttggatca tcatgataat tcttatactg caccatttct cccagcataa tgaccaatcg
     8761 atcaagggag cggctggcag accaccaggc ggcgtcgatg cagacgctgc cattggcgtg
     8821 attgatgttg ggatgccaga tgggggtcaa ccatttcatc cctggccagc gttggggata
     8881 ttgattgtgt agatagatct caacctggtg cttatctcca aattttgggt ttccttttcg
     8941 atcaatccca atgattcctt tacagctaaa tgtaatgata tatttttcag ggggaagatt
     9001 tccaggggtt ttctccgctt ttacttgaat caggtcactg cgcgcgttga tttcctggat
     9061 cagctcataa tcttttttca accggcgcat ccgcggactt gaagaaacag aaacggcacc
     9121 ggcggtgatg tcggtggtga tcatcaggcg atcatcatgg ggtatatgtg cctgaccgag
     9181 cgtttccgat tcgcgcaatt ctctccccag tgctttgctg tccatgcggt aacccagcgg
     9241 tcgtccatct ggacccacag tgggtagccc cagcaacgaa gtcaattcgg tcagcaaatc
     9301 acggaccgtt acgtcatcag gcatttctgc ttttcttgcc ccgccattgg gcaacaccaa
     9361 cgttactgaa atatctgcca tacactcctc ctgggatgaa atctatagtt catgtttgtg
     9421 cgctatctgg caggaaccgg tgatttccca gcaatcccgg tgatgccatg ttccacaatc
     9481 aggacagatc atgaccccgc gcggatcgtt cttatgcacc tcttccagac aataagggca
     9541 gtggtggtct tctgtgccag tcaatttcac cttctttcga ctccacattc tggtcgtggg
     9601 tgctctcttt gcagaacttt tcttggtcga cttgaccctg agcgcttgtg aagattgttt
     9661 cggactggag ttggtgatga cttttttctg tgttcctgtt tttctaatca ccttcctctt
     9721 ggtcagagct ttcaaatcaa tgatcggttt tctctttttc tttattgcca ccgacttgac
     9781 aggcaaagaa gctggttttc gttttgcaac gttcttctgc gattcaagtt tggaggtctc
     9841 tttgtgcact actgacacag gttttttctt gccgatcaac gcaatgaaag cggtgattat
     9901 taacaccaga ccgatctgag tgtattctcc aatagaccac catgcaatct gaagggttcg
     9961 atcacgcagc agcgcaaaag cagttgggtt gtaccagtcc aatgccgcaa tgctgagcag
    10021 actgccaaag gagccaaagc atatttttaa gaaccacatc ctgaccggca cggtcagccg
    10081 gcaaaccagc cccactccaa tgccaaccac cagcaagatc aaagcccggg ttatcaattg
    10141 gctgatctgg gtttgcgggg tcaggtagta gagaatgagg tatcctgatc cgaaacctac
    10201 gctgaggatc aacaaaaaaa tcaagaaacc gtgaaaaaaa tttatctttt tcatttgatc
    10261 gttttatcgg tttctaattt gatccttgat ctggtggatg gttttgcccc cgttttttca
    10321 tcttcagtgg ttgctatctt tatcactgga acatcatcca gtttaatgac cggtttctgg
    10381 aagtgcttaa aatgcaaagc gttatccaga tcgccggata attcataaaa agcatactgg
    10441 tcggcgttat aagcgcgcat gatgtggagt ttgggcacac ccaagctgaa aagggtgcgg
    10501 ttgatgtatg gttcttcgcc agtgacgata tgggtcattt cggtttcgcg caacatgccg
    10561 caggtggggc aatgcgcatg gttaaagccg acctgcgaga tcggttgaag gatatcctct
    10621 tttgtttggc aattgcggca atataacgtg agaatcagct cctgatccag ctccaatact
    10681 gcctgtgggc ccaaatcacg gtggatgatt ttgagcatat cttcaagggt ggttgtatca
    10741 gcccgcaatg gaagttcggt gatcttgccg tatgtccaat ggctttcgca atcttctgcg
    10801 ggggtgtagc tggttttgtg catttcgtta gtcatgccat taaagtggat aaccttgcca
    10861 gcttctactg gtttcccatg gatgagcttc aatgcttcct gtgattggat ggcagcgatg
    10921 atggaggcaa tggtgggcgt ggtgggcact ttgccaagca gtatgttttc tctggcaagc
    10981 agagggcagg aatagcggat ggaaagatcc cgccgggctt gttcggtgag cgtgcattca
    11041 aagcaggcgc cttcccccgg cacgaaaacc cgtgccagcc caaagaattc ctgaatggcg
    11101 ccatctaccc aagacttgtt catccaatag gcaaaacgat ttaccgctaa tcgggcttct
    11161 cggttgtcaa gacagccgat gatcacatcc atccgacgaa agacgcctaa tccgatatcg
    11221 gtggtcacgt caccgtgcag gtattggacg tgcacattgg ggttgatctc ttttgcccgg
    11281 gcggcagcca cttcggcttt ttttctgccc ttgtcgctct cacgaaaaag aacggagcgt
    11341 gagaggtttg ccatttcgat ggtgtcgaaa tccacgatat aaagatagcc gattcccatc
    11401 aataccagat tcttgatcac ctcgttgccc agtg
//
LOCUS       DAOSDS010000069        11341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2198_length_11341_cov_3.683856, whole genome shotgun sequence.
ACCESSION   DAOSDS010000069 DAOSDS010000000
VERSION     DAOSDS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2198_length_11341_cov_3.683856"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLL88_08250"
     CDS             <1..518
                     /locus_tag="PLL88_08250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOJ01594.1"
                     /translation="LKEEKVKQIVMLTGDAKLIGEKVASQLGLDQVYTELFPADKVQK
                     VEQLFAEKSSKGKLAFIGDGINDAPVLARADIGIAMGGLGSDAAIEAADVVIMTDEPS
                     KIATAMKISRKTLHIVKQNITIALTVKAAVLILGALGIASMWAAVFADVGVALIAILN
                     AVRILNVKKFQ"
     gene            663..986
                     /locus_tag="PLL88_08255"
     CDS             663..986
                     /locus_tag="PLL88_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01595.1"
                     /translation="MFGAGWLNIGSLVLGLIAWMLPVVNLAQHNKADHGHWGVFSMAS
                     VSACAISLCMQIFYTDHLVKIEDWSALMDTSHAVALVATLLLLVTIVLNGITVSVYCG
                     KKPKG"
     gene            1124..1420
                     /locus_tag="PLL88_08260"
     CDS             1124..1420
                     /locus_tag="PLL88_08260"
                     /inference="COORDINATES: protein motif:HMM:NF026248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HOJ01596.1"
                     /translation="MNFEGIVIGAGAFMIIGILHPVVIKTEYHIGARAWPMFLITGLL
                     CIGLSLFWDSSVPSSLLSILGFSLLWSIQELFQQEKRVAKGWFPKNPKKDHSLR"
     gene            1493..1780
                     /locus_tag="PLL88_08265"
     CDS             1493..1780
                     /locus_tag="PLL88_08265"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01597.1"
                     /translation="MSILLEKKTYSNGQKVFDWDGTTLTYYFKNGEVKTKGQYINEQM
                     EGEWLFYRESGQLWQVGNFKNGKKHGTWKRYSKENRLEYEQEFVDGKPAKK"
     gene            1784..2467
                     /locus_tag="PLL88_08270"
     CDS             1784..2467
                     /locus_tag="PLL88_08270"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOJ01598.1"
                     /translation="MKTAGVIGGFGPQTTAKFYEEVFFACQRLNSVVKPHILISSVPL
                     PFWIEETALLKNEGVEDYLPYLTKEAQRLEKAGADFIVMPCNSLHVHIDALCASVQLP
                     VLSILEETAQFLVQQNIHRIGILSTAITASHFLYKNALAKYGIECLHPKQDQQEFLNA
                     IVLRLVNGITDNHDREVLHAIMQTLTGVDCILLACTDLQLVELHYPTLPIFDTLQILV
                     NSTVRELLA"
     gene            complement(2512..3693)
                     /locus_tag="PLL88_08275"
     CDS             complement(2512..3693)
                     /locus_tag="PLL88_08275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOJ01599.1"
                     /translation="MGEPILVLKKDREKPIIAGHPWIYSGAVERMEGEAGMGETVLVK
                     DGWDNCIGRAAYSPASKIRARMWSWDAEEAIDDSFIKNKLRIAISLRKHINANAYRLL
                     NAESDYFPGLVVDRYADYLVLQSLTAGVEHWKDTIVKGLVELTGIPNIYERSDVEVRR
                     LEGLTERCGVLAGKEPEKFVEIRENDLKFQVDICSGQKTGFYLDQRENRARILSYVAK
                     KSVLNCFCFSGAFGVYAVKGGASQVCSIDSSDDAIELAKNNARLNFPKYENLEWVVAD
                     VFEYLRQLRDQGRMFDVVILDPPKFAPTRKLVEQAARGYKDINLLAMKLLNPDGILIT
                     FSCSGGISAEFFQMILKEAIADAKRQVFIQERLFQSSDHTTSLHFPEGEYLKGFICKV
                     R"
     gene            complement(3686..5710)
                     /gene="ligA"
                     /locus_tag="PLL88_08280"
     CDS             complement(3686..5710)
                     /gene="ligA"
                     /locus_tag="PLL88_08280"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOJ01600.1"
                     /translation="MNDTQLHDELERLKQEIHFHNYRYHVLDDPLISDVNFDQLLKRV
                     QEIEALHPEWITADSPTQRAGAQPAERFEKTRHPHSILSLANAFSNQGVLDWYDRIAR
                     LDARVRNARFVVEPKIDGLTVVLHYENGIFVRGATRGDGEIGEDITGNIRTIRALPLR
                     IPVDRTGPAAPARLVVRGEVFMNLADFEKLNRALEEQGLRTYQNPRNTAAGSLRQLDS
                     GLTASRPLTVLTYAIVAMEGDMPSSQWELLNALRAWGFPVTGNATLCEDIQSVLQVCD
                     SWLEKRNHMPYEIDGLVIKIDDLGLQDALGFVGKDPRGAIALKFPAKEATTRLIDIKV
                     NVGRTGVLTPYAVLEPVEIGGVIVKQATLHNFDYIREKDIRVADMIRIKRAGDVIPYV
                     IGPILELRKGNEKIFVPPTYCPSCGAPSAHIEGEVAWFCVNAACPAQLIRNVEHFVSK
                     GAMNILGMGIRYVEELVQSGLVKDVADLYRLTESDLLQLEGFAEKKARNLLDAIQTSK
                     SQPLARVITALGIRGVGEIAAMELAAHFKSLDTLSQATFADLTSMEGFGPNISQAILD
                     WFNNPRNQQLLQKLKAIGIHPEQEEQSQKVLPLDGMTFVITGTLPSLSRDEATEFIRA
                     QGGKVTGSVSKQTTYVLCGSEPGSKYDKAIELNIPVISEDDLRKLVAQNG"
     gene            5815..6177
                     /locus_tag="PLL88_08285"
     CDS             5815..6177
                     /locus_tag="PLL88_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01601.1"
                     /translation="MKAGKIILFVLAAVLIFFGVIFLLSISASGDVNLIWYAAVFFLI
                     AFILIFFAARIKKPSEQTTNVTLNIDLPGDVKMETIKCKSCGSPLSEGDVKMVNGAPM
                     VTCSACGTSYQLTEEPKW"
     gene            6201..7940
                     /locus_tag="PLL88_08290"
     CDS             6201..7940
                     /locus_tag="PLL88_08290"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOJ01602.1"
                     /translation="MKTFQRVVTVLFLIAVLAMSTPVYAQNYSFQVPSLTTDISINED
                     GTATIEYLILFDNDAGAAPIDYVDIGMPNESYALNNIYATIDGKTITDIQDSPYVEYG
                     FALGLGVDAIPAGQQGTVYVRVESVSDMLFVATTEGKTEEYVSFNFSPAWFDASLSHG
                     STEMTVTVHLPAAVVDPEPTYIQPKNWPGDPTPLTGFDEEGRIVYQWSTTEANPSTQY
                     QPFGATFPARYVPSTTISQDNVITFNSDRLWSILIPVLCCGGGIAIVVLLIVVALKAS
                     KNRKLKYLPPKISVEGLGIKRGLTPVEVGILMEQPMDKILTMILFSTMQKEAVQIITR
                     EPLEVKVEETLPEDLHQYEKEFLEAFRPKDTRERTRALQEMMVNLVKAVSEKMKGFSR
                     KETLDYYKDIMEKAWQQVESADTPEVRGERYQEQMDWTMLDKDYDTRTRNVFGTGPVY
                     MPWWWWRADPGMARTTPTIGTSGSKTASAPSGGSKSSGSNMTTVRIPHLAGSNAAATL
                     VGTVQSFSAGVVGSIASFTDKVTNKTNPIPQSTSSSRSFRSFSGGGRPSGGGCACACA
                     CAGCACACAGGGR"
     gene            7958..9307
                     /locus_tag="PLL88_08295"
     CDS             7958..9307
                     /locus_tag="PLL88_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ01603.1"
                     /translation="MLFAKRDHTVRLEPGIYHYLRSEGENKKRIHLRIDPDGYSTLLV
                     NASHIFHFNPSASLMAKAYLDEVPTDEIIHGLVRVFQVNKNQATQDYAMFQEQLELIT
                     SPASDACPFCDLNVDIQAPFSKQPSAPYRMDLALTYACNNDCAHCYNVKQRHLHALPL
                     EEWKQVLQKVWDCGIPHVVFTGGEPTLVEYLPQLIRHAEELGLITGLNTNGRALKDPS
                     FVQRVLDAGLDHVQITLESHDPTIHDRMVCHSGAWQETVQGIRNALKTRAFVMTNTTL
                     IKSNSVHLSETLTFLKELGVPTVGLNGIIHSGRGQQFREELDESELPALLHTAQQMTA
                     RNGQQLIWYTPTQYCHFDPVMMGLGVKGCTAALYNMCVEPNGDVLPCQSYYTPLGNIL
                     RDDWKNIWEHDLAVSIRERHFLPEGCRDCAILKTCGGGCPLATAEKPAVNPQYVLQS"
     gene            9325..10485
                     /locus_tag="PLL88_08300"
     CDS             9325..10485
                     /locus_tag="PLL88_08300"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ01604.1"
                     /translation="MFTKIKDKVEKLIEPVKPVPVGSYQYHGVKGDRLPYRLNLRVEP
                     SGEGILIINASTVLHLNRTAAEYAYHLIQNTPIEQAAQEVADKYDISPEEMKRDFADF
                     RAQLDTIIHTPDLDPVTYLDLERVEPYSKKLNAPYRLDCALTYRLSEGATQDDAPTYR
                     VKRELTKEEWFTILEKIRQAGIPQVIFTGGEPTLRSDLPDLVAQCEKLGIVCGLLTDG
                     LRFADKGYMRSVLNKGLDHIMLLGAPRDDRFWKSLKNLMDDDIAVTVHLTLTSEDHSE
                     LEEALPKLATSGLRSLSLSASDPHHEKHLQKLQEQAIAQGLSLVWDLPVPYSQYNPVN
                     MELKSKGKRMAKGAGNAWLYVEPDGDVLPAQGINRVLGNLLDDPWSSIWRKR"
     gene            10498..11265
                     /locus_tag="PLL88_08305"
     CDS             10498..11265
                     /locus_tag="PLL88_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ01605.1"
                     /translation="MDFTPRFWHKRFIQQAGWTKEVRRYILQTLRAARTTPILEVGCG
                     TGAVLSQIYQDGSHRLFGVDIGLPGLEFARSRNPHLRMTCADGLRLPFREQRFAITLC
                     HYLLMWISDPMAVLEEMKRVTRSGGYLVVLAEPDYAARQDEPAELRKLGELQNMALLA
                     LGANIEIGGQVRPLLEKLRLEDIHVSMLKPAFTRPAARDESLEWKVIAFDLQQLVSRG
                     ALDQQQVERFRLLDERARHEGTRRLFIPTYFGWAKVP"
BASE COUNT         2913 a         2890 c         2705 g         2833 t
ORIGIN      
        1 acctaaaaga ggagaaggta aagcagatcg ttatgttgac cggcgatgcg aagctgatcg
       61 gcgaaaaggt agcatcacag ttgggtttgg accaagttta taccgagttg tttccagcag
      121 ataaggtgca aaaggtggag caactctttg ccgaaaaatc atcgaagggc aagctcgcct
      181 ttataggcga cggaatcaac gacgctccgg tcctggccag ggctgacatc ggcatcgcca
      241 tgggtggctt gggctcggat gccgccatcg aagcggctga cgtggtcatt atgactgacg
      301 agccctccaa aatcgctacc gccatgaaaa tatccaggaa aactttgcat attgtaaagc
      361 agaacataac catagccctg acagtcaaag ctgcggtgtt gattctggga gctttaggca
      421 tcgcgtccat gtgggcagcc gtctttgccg acgttggcgt tgcgctgatc gcgatcctta
      481 atgcggtccg catcctgaac gttaaaaaat tccagtaaca acaaagtcag gagtattatc
      541 taactcctgg ctttgttgtt tttatgcaag gagataaaaa taagaaaaag aaggcttcac
      601 taaagaaagc atagtatgat ggatgcaaag gtcattatgc tatacggata agggagaaaa
      661 ttatgttcgg agccggttgg ctgaatatag gcagtcttgt attggggttg attgcatgga
      721 tgctgcctgt tgttaatctt gcgcaacaca acaaggctga ccacgggcac tggggcgttt
      781 tctctatggc aagcgtcagt gcctgtgcca tctcattgtg tatgcaaatt ttctataccg
      841 accatctggt aaagatagag gattggtcag cgctcatgga tacttcccat gcggtggcat
      901 tggtcgccac cttactgctc ctggtcacaa ttgtgctcaa tggaattaca gtaagcgtat
      961 attgtgggaa aaagccgaag ggctgataaa ctccagtttt ccggttgttg tcgaatagaa
     1021 gttacttata ctttgcagag gatacatagt atcacgccaa tttgatacaa tattgacaat
     1081 ccaaaaaaac ttggtaattc tgtaaagtgg aaggagtcag ggcatgaatt ttgaaggaat
     1141 agtcataggt gccggcgctt ttatgatcat cggcatctta caccctgttg tgatcaaaac
     1201 tgaataccat atcggcgcaa gagcatggcc catgtttcta ataacaggtt tgctgtgcat
     1261 tggtctgtcg ctcttttggg atagctcagt tccgtcatcc ctactgtcta tattgggttt
     1321 ttcgctgcta tggagtattc aggaactttt tcaacaagag aaaagggttg caaaaggatg
     1381 gtttcccaag aacccaaaga aagatcattc attgaggtga tatacatcaa ttatcattct
     1441 gaccatacga tatccggcgg aagaattaat agaaaccaaa agaaccaagg aggtgagcat
     1501 tttacttgaa aagaaaacct attccaatgg gcaaaaggtg tttgattggg atggcaccac
     1561 tctgacatat tatttcaaga atggtgaggt taaaaccaaa gggcaataca tcaacgaaca
     1621 gatggaaggt gagtggctgt tttaccgcga aagcggacag ctctggcaag ttggcaattt
     1681 caaaaatggg aaaaagcatg gtacctggaa acgctatagt aaagagaatc gactcgaata
     1741 tgagcaggaa tttgtagacg gtaaaccagc caagaaataa ataatgaaaa cagcaggtgt
     1801 cattggtggg ttcggcccac aaaccacagc aaaattctac gaggaagttt tctttgcctg
     1861 tcagagactg aattccgttg taaaaccgca catcctcatt tccagcgttc cactgccctt
     1921 ttggatcgaa gagactgcac ttttaaaaaa tgaaggggtg gaagattatc ttccttattt
     1981 aacaaaggaa gcgcaaagat tggaaaaagc gggggcagat ttcattgtaa tgccctgcaa
     2041 ttccctccat gttcatattg acgcactgtg tgccagcgtt cagctccctg ttctttccat
     2101 actggaagag acagcacaat ttctggtcca acagaacatt cacagaatcg gcattctttc
     2161 aactgccatc accgcttctc attttttata taaaaacgcc cttgcgaaat atggaattga
     2221 atgccttcat ccaaagcaag atcaacaaga attcttaaat gcgattgtgc ttcgcctcgt
     2281 aaacggtata acggataacc acgatcgtga agtgttgcat gcaatcatgc aaacgttaac
     2341 cggtgttgac tgcatcttgc tcgcctgtac tgatctccag ctcgtagagc ttcactatcc
     2401 taccctgcca attttcgaca cgctgcaaat tttggtcaat tccactgttc gtgaattatt
     2461 agcctaaatt caattagcac cctatcattc atggctttgg caattatctc gctaacggac
     2521 tttacagata aaccctttta gatattcccc ttctggaaag tgcaagctgg tggtgtgatc
     2581 gctgctctga aacaaccgtt cttgtatgaa aacttgccgt ttggcatccg ctattgcctc
     2641 tttcaagatc atttgaaaga actcagcact gattccgccc gagcaggaaa atgtgatcaa
     2701 gattccatcc gggttgagca gcttcattgc cagcaagtta atatctttgt accctctggc
     2761 agcctgttca accaacttgc gggtgggggc aaatttcggc ggatcaagaa tgacgacatc
     2821 gaacattcgc ccttgatctc gcaattgacg caaatattca aatacatcag ccaccaccca
     2881 ttccagattc tcgtattttg ggaagttcag cctggcatta ttttttgcca attcaatcgc
     2941 atcatcagaa gaatctattg agcagacctg gctggcacca cctttgaccg cgtacacgcc
     3001 gaaagcgccg ctgaagcaaa aacaattcag cacactcttc ttcgctacat aagacagaat
     3061 tctggctcga ttctcccgtt gatcaagata aaaacccgtt ttttgtccgc tgcaaatatc
     3121 tacttgaaac ttcaaatcgt tttctctgat ctcaacgaac ttttcaggtt ccttccccgc
     3181 caacactcca caacgttcgg ttaatccttc taatcttcgc acttccacat cggagcgttc
     3241 atagatgtta ggaattccgg tcaactcgac caagcctttc acgatcgtat ctttccaatg
     3301 ctctaccccc gcggtgaggc tctgcagcac gagataatca gcatatcgat ctactaccaa
     3361 acctggaaaa taatcggatt ctgcgtttaa aagacggtaa gcgttagcat taatatgttt
     3421 tcgtaaggaa atggctatgc gcaatttgtt cttgatgaac gaatcatcga tcgcttcttc
     3481 agcatcccaa ctccacatgc gcgcccgaat cttggacgca gggctgtacg ccgccctgcc
     3541 aatgcagttg tcccacccat ctttcaccag aaccgtctcg cccattcctg cctctccctc
     3601 catccgttca accgcaccgg aatagatcca gggatgtcct gctatgatcg gtttttctcg
     3661 gtcctttttc aatacgagaa tcggttcacc cattctgtgc taccaacttg cgcagatcgt
     3721 cttcactgat aaccggtata ttcaactcaa tagccttatc atatttcgag ccgggctcgc
     3781 ttccacatag cacataggtg gtctgcttgc tgaccgaacc ggtcactttc cctccttgag
     3841 ctcggatgaa ctccgtcgct tcatcacggc tcaaagaagg taatgtgccc gtgatcacaa
     3901 aagtcattcc atctaaaggt aaaactttct gactttgctc ttcctgttcg gggtggatgc
     3961 caatcgcttt cagcttttgc aataattgtt ggttacgcgg attgttaaac caatccagga
     4021 tcgcctggct gatgttcggt ccaaatcctt ccatgctggt caggtcagca aacgtcgcct
     4081 gcgacagggt atctagactc ttgaaatgcg ctgccagctc catagcggca atttccccaa
     4141 ccccgcgaat gcccagcgcg gtgatcaccc tggcaagggg ttggcttttg gacgtctgaa
     4201 tggcatccag taaattacgc gcttttttct cggcaaaacc ttccaattgg agcaggtcac
     4261 tctcggtaag ccgataaaga tcggctacat ccttcaccaa tccactctgc accagctctt
     4321 ccacatagcg aatgcccatg cccagaatgt tcattgcccc ttttgagaca aaatgttcca
     4381 cattccggat caattgcgcc ggacaggctg cattgacaca aaaccacgcc acttccccct
     4441 caatatgtgc gctcggtgcg ccgcagctcg ggcagtacgt tgggggtacg aatatttttt
     4501 cgtttccctt gcgcaactca agaatcggtc cgatcacata gggaatcaca tcgccagcgc
     4561 gcttaattcg tatcatgtcg gctacacgaa tgtctttttc cctgatatag tcaaaattat
     4621 gcagggtagc ctgtttgact atcaccccgc cgatctccac cggttccaac accgcatagg
     4681 gggtcaacac ccctgtgcgc cccacattca ccttgatatc gatcagtctt gtggtggctt
     4741 ctttcgcagg aaactttaac gcgatcgccc cgcgcggatc cttgcccaca aaaccaagtg
     4801 catcctgcaa acccaggtca tcgatcttga tcaccagacc gtcaatttca taaggcatgt
     4861 gattgcgttt ttccagccag ctatcacaaa cctgcaagac tgattggatg tcttcacaca
     4921 gcgtcgcgtt tccggtaacc gggaaacccc atgcgcgcaa agcgttgagc aattcccact
     4981 ggctggaggg catatcccct tccattgcga caatcgcata ggttaatacg gtgagcgggc
     5041 gcgatgcggt caaacccgaa tctaactggc gcaaggaacc cgcggcagta ttgcgggggt
     5101 tctgataggt tcttaacccc tgctcctcca atgcgcggtt taatttttca aaatcagcca
     5161 ggttcatgaa aacctcgccg cgcaccacaa ggcgtgccgg ggctgccggt ccggttctat
     5221 caacaggaat gcgcaatgga agcgcccgga tggtgcgtat atttcctgtg atatcttcgc
     5281 caatttcccc atcaccgcgc gttgcgccgc gcacgaaaat tccattctca taatgcagca
     5341 ctaccgtcaa tccatcaatc ttgggctcca ccacgaaccg cgcatttctc acccttgcat
     5401 ccagtcttgc aatgcggtcg taccaatcca gcacgccctg attggaaaaa gcattagcca
     5461 ggctaagaat agagtgtggg tggcgcgttt tttcaaaacg ttcagccggt tgagcgcccg
     5521 cgcgttgtgt cggtgaatcc gccgtgatcc attctggatg cagcgcttct atttcttgca
     5581 ctcttttcaa caactggtcg aaatttacat cactgatcag aggatcatcc agcacatgat
     5641 agcggtaatt atggaaatga atttcctgct tgagtcgttc aagctcatcg tgcaattgtg
     5701 tgtcgttcat tgatcgtatt atagcaataa ttggttttcc cgactcgtaa gaacttctcg
     5761 cgtttatttc cgtatataat agatgacgat tgaaaatgtt tttttggagg tctcatgaaa
     5821 gctggaaaaa tcattctctt tgtgcttgcc gcagttctca ttttctttgg ggttatcttc
     5881 ttgctctcca tttctgcaag tggtgatgtc aatttaatat ggtatgcggc tgtatttttc
     5941 ttgatcgcat ttatcctgat cttttttgcc gctcgtataa agaagccctc tgaacaaact
     6001 accaacgtaa ccctgaatat cgatctgccc ggcgatgtga aaatggaaac gataaaatgc
     6061 aaatcttgcg gttcgccgtt atcagaaggc gacgtgaaaa tggtcaatgg tgcccccatg
     6121 gttacctgtt cggcgtgcgg cacctcatac cagctcaccg aagaacccaa gtggtaattg
     6181 aaggaaagga aacgatttcc atgaagacct ttcagcgtgt tgtaactgtg ctctttctga
     6241 tcgccgttct ggcgatgagc acacctgtct atgcgcaaaa ctactctttc caggtaccat
     6301 cccttaccac cgatatttct atcaatgaag atggtactgc aaccatcgag tatctcatcc
     6361 tttttgacaa tgacgcaggg gcagccccca tcgattatgt tgacattggc atgcccaacg
     6421 agagttatgc tctcaacaat atctatgcca ccatcgatgg aaaaaccatc accgatatcc
     6481 aggattcgcc ctatgtggaa tacggttttg ccctcgggct gggtgttgat gccatcccgg
     6541 ctggtcagca gggcaccgtt tacgtgcgcg ttgaatctgt gagcgatatg ctctttgttg
     6601 ccaccaccga agggaaaacc gaggagtatg ttagcttcaa cttctcaccc gcatggttcg
     6661 atgcctctct cagccatggc agcaccgaaa tgaccgtgac cgttcacttg ccagccgcgg
     6721 tggtcgatcc tgagccaacg tatatccaac caaagaactg gccgggtgac cccacacccc
     6781 tgacagggtt tgatgaggaa gggcggattg tatatcaatg gtccacaacc gaagcaaacc
     6841 catcaaccca gtaccaaccc ttcggggcta ccttccccgc ccgctatgtt cccagcacta
     6901 caatatcaca agacaatgtg attactttca atagtgatag gctatggagt attttaatcc
     6961 ccgtgctatg ttgcggcggg ggaatcgcaa ttgtggtgct cctgattgtc gtggcattaa
     7021 aggcatcgaa aaatagaaaa ctaaaatacc tcccgccgaa aatatcggtg gagggtctgg
     7081 gtatcaaacg cggtctcacc cccgttgaag tgggcattct catggaacaa cccatggata
     7141 agatcctcac catgattctt ttctccacca tgcaaaaaga agccgtgcag atcatcactc
     7201 gagagccatt ggaggtcaag gttgaagaaa ctttgcctga agaccttcat cagtatgaaa
     7261 aggaatttct tgaagctttc cgcccgaaag acacccgtga gcgcaccaga gcgctgcagg
     7321 aaatgatggt gaacctggtg aaagccgttt ctgaaaaaat gaaagggttc agtcgcaagg
     7381 aaacgctcga ctattacaaa gatattatgg aaaaagcctg gcagcaggtc gaatctgctg
     7441 atacgccaga ggtgcgtggt gaacgttatc aggagcagat ggattggacc atgctcgata
     7501 aagattacga cactcgcacg cgcaacgtat ttggtaccgg accggtatac atgccctggt
     7561 ggtggtggcg ggctgaccct ggcatggcac gcactacccc caccattggc acctccgggt
     7621 caaagacggc gtcagctccc tctggcggtt ccaagagcag cggaagcaac atgaccacag
     7681 tgcgcatccc gcatctggct ggttctaatg ctgccgccac actggttggt acagtgcagt
     7741 ccttctccgc cggcgtggta gggagcatcg ctagcttcac agataaagtc accaataaga
     7801 ccaatccaat cccgcaatct acatcttcaa gtcgatcctt ccgctcgttc agcggcggag
     7861 ggagaccttc gggtggtggt tgcgcctgcg cctgtgcgtg tgccggttgt gcctgcgcct
     7921 gcgcaggtgg tgggcgatag aatagatata caaatatatg cttttcgcaa aaagagatca
     7981 cacggttaga ttagagccag gtatttatca ttacctgcgt tcagaaggtg aaaataaaaa
     8041 acgtattcac ctgcgcatcg accctgatgg gtattctacc ctgctggtca acgccagcca
     8101 cattttccat ttcaacccta gcgcatcgtt gatggcaaaa gcatatctgg atgaagtgcc
     8161 gaccgacgag atcatccatg gcttggtacg ggtcttccag gtcaacaaga atcaggcaac
     8221 tcaagattac gccatgttcc aggaacagct cgaattgatc acctcacctg ccagcgacgc
     8281 ctgccctttc tgtgatctca atgtagacat ccaggcgccg ttctccaagc agccttcagc
     8341 gccataccgt atggatctgg cgttgacgta cgcctgcaac aacgattgcg cgcattgtta
     8401 taacgtgaag caacgtcatc tgcacgcttt acccctcgaa gaatggaagc aggtgttaca
     8461 aaaagtttgg gattgcggta tcccgcatgt ggttttcacc ggcggggaac caacgctggt
     8521 ggaatatctg cctcaactca tccgtcacgc ggaggaattg gggctcatca ccggattgaa
     8581 caccaacggg cgcgctttga aagatccatc attcgtgcaa cgggtattgg atgcggggct
     8641 cgaccatgtg cagatcacgc ttgaatcgca cgatcccacc atccatgatc ggatggtctg
     8701 ccacagcggc gcctggcaag aaaccgtaca gggaattcgg aacgctctga aaacaagggc
     8761 attcgtgatg accaatacga ccctcatcaa gagcaacagc gtgcatctat ccgaaacgct
     8821 gacatttctg aaagagctgg gagttcccac cgttggattg aacggtatca tccattcggg
     8881 cagagggcaa caattccgtg aagaacttga tgaaagcgag cttcccgctt tattgcacac
     8941 ggcacagcaa atgaccgcgc gaaatgggca acagctcatc tggtacaccc ccacccagta
     9001 ctgccatttt gatccagtca tgatgggatt gggcgtcaaa ggctgcactg ctgccctata
     9061 taatatgtgt gtagaaccga atggcgatgt gttgccctgt caatcgtatt acaccccgct
     9121 gggcaatatt ctgcgggacg attggaagaa catctgggag catgacctgg ctgtttccat
     9181 tcgagaaagg catttcttgc cagaagggtg ccgggattgt gcaattctga aaacctgtgg
     9241 gggcggctgc cccctggcaa cagcagaaaa acctgcggtt aacccgcagt atgtcttaca
     9301 gagttagatg gttatggagg gtccatgttc acaaaaataa aggataaggt tgaaaaactc
     9361 atcgaaccgg tcaaaccggt gccggtggga tcctatcaat atcacggcgt aaaaggcgat
     9421 cggctcccct atcgcctgaa ccttcgcgta gagcccagcg gagaaggcat tctcattatc
     9481 aatgccagca cagttttgca tctcaaccgc accgccgctg aatacgccta tcacttgatt
     9541 cagaacactc ccattgaaca agctgcacaa gaggtcgccg ataagtatga catcagccca
     9601 gaagaaatga aacgcgactt cgctgatttc cgcgcgcaac ttgataccat catccatacc
     9661 cccgatcttg atccggtcac ctatctagat cttgagcggg tagagcccta ctccaagaag
     9721 cttaatgctc cctaccgcct ggattgcgcg ctcacctacc gcctctccga aggcgcgaca
     9781 caagacgatg ctcctaccta ccgggtgaaa cgggaactca ccaaagaaga atggttcacc
     9841 attctcgaaa aaattcggca ggccggcatt ccgcaggtaa ttttcaccgg tggggaacct
     9901 acccttcgtt ctgacttgcc cgacctggtt gcgcaatgtg aaaagctggg aatcgtgtgt
     9961 ggtttgttaa ccgatgggct gcgttttgcc gataaagggt acatgagatc cgttctgaat
    10021 aagggacttg accacatcat gctgttgggc gctccgcgag atgatcgctt ttggaagagt
    10081 ttgaagaacc tgatggatga tgacatcgcg gtaaccgtac atttgactct gacatcagag
    10141 gatcattctg aactggaaga ggcgctgcca aaactggcaa cctcaggctt gcgctcctta
    10201 tcgttgagcg catctgaccc gcaccatgaa aaacatttgc agaaattaca ggagcaagct
    10261 attgcgcaag gtctttccct ggtttgggac cttccggtac cttattcaca atacaatccc
    10321 gtcaacatgg aattgaaaag caaaggaaaa cgcatggcaa agggcgccgg gaacgcttgg
    10381 ttgtacgtgg aaccggatgg agacgtgttg ccagcccagg gcatcaatcg cgtgttgggc
    10441 aacctgctgg atgatccctg gtcttccatc tggaggaaga gataagggag cgctccggtg
    10501 gacttcactc cgcgcttctg gcacaaacgc tttatccagc aagctggatg gacaaaggaa
    10561 gtccgccgat atatactgca aaccctgcgc gccgcacgca ccacaccgat cttagaagtg
    10621 ggttgcggta ccggcgctgt tctaagccag atataccagg atggcagcca tcgtcttttc
    10681 ggggtggata ttggattgcc cggtttggag ttcgcgcgca gtcgcaatcc ccatttgcgc
    10741 atgacctgcg ccgatggact cagactgcct tttcgggaac agcgcttcgc gatcacgctc
    10801 tgccattatc ttttgatgtg gatttccgat ccgatggctg tactggaaga aatgaaacgc
    10861 gtgacgcgtt ccggaggata tctagtggtg ctggcagaac cggattacgc tgccaggcaa
    10921 gatgaaccgg ctgaactgcg caaactgggt gaattgcaaa acatggcatt gctcgcgctt
    10981 ggggctaaca tcgaaatagg tggacaggtg cgtcctttgt tggaaaagtt gcggctggag
    11041 gacatccacg tgagcatgtt gaaacctgct ttcacgcgtc cagcagcccg cgacgaatca
    11101 ttggaatgga aagtgatcgc ttttgatctg caacagttgg tgtcacgcgg tgcacttgac
    11161 cagcaacagg tagagcgatt ccgcttatta gatgagcgcg cgcggcacga aggaacccga
    11221 cgcttgttca ttcccaccta ttttggttgg gctaaagtac cttaatgcac caatttcccg
    11281 atcttcctga acaagttcag atagatatga gagaggggag ctagacagga acggcaatcg
    11341 g
//
LOCUS       DAOSDS010000070        11094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2257_length_11094_cov_4.677416, whole genome shotgun sequence.
ACCESSION   DAOSDS010000070 DAOSDS010000000
VERSION     DAOSDS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11094
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_11094_cov_4.677416"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..1615)
                     /locus_tag="PLL88_08310"
     CDS             complement(245..1615)
                     /locus_tag="PLL88_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOJ01606.1"
                     /translation="MNEVLKKNYYLPNDKPPFGRMLLLGLQHVLTMFPATVLVALLVG
                     FHINTVLLASGMATLVALILSKFKIGTFIPLYYGSSFSYLAAIMAITKAQYGVVASDE
                     LIRTAQAGIVCTGVINILVGLLVHYCGGKNFINRILPPVVTGSVACVIGFSLGYTALQ
                     NASGGAFGIVDNGSWWLVAFITLLATIFFSVFLRGKGFIGMLPILLGAIVGYVVSIPL
                     GLVDFSVIAEANFFEVPHITLPSFTSDLSLTAIASIAIMAIATIPESTAHLYQIGLYV
                     DKLAEGKGRQKVGLSNHVGFNLVLDGLGDLINGLIGGAAGTNYGENNSLMVITQNYSG
                     YTLVCAAVIAMILSFSGILSGIVRSIPTAVTGGLSIYLFGVIGMQGVALMIEEKVDLF
                     DPRNLAIGAVIFIIGVGGHIGYANGLMPVPLLRSVFPDGWPAIATGALLGIALNLVFR
                     LFDKKK"
     gene            complement(1847..2164)
                     /locus_tag="PLL88_08315"
     CDS             complement(1847..2164)
                     /locus_tag="PLL88_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01607.1"
                     /translation="MAIQVLKNDLRPFRSMMKPFTFIFWRLPLRIVRQEIMELSFGAI
                     VFLFFMKLYYGLLYRLAPMLQRINPRAFRQLHFRISFFFYRKIVPPPVVLLHAALNLY
                     KKL"
     gene            2249..2971
                     /locus_tag="PLL88_08320"
     CDS             2249..2971
                     /locus_tag="PLL88_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ01608.1"
                     /translation="MTKSNLAKENYSSFAERYAQGIAANSHNAFYERPATLSLLPEVK
                     DLRIFDAGCGPGIYSQWLIEHGAHVTAIDATPEFVQMAQDRTQQKANVLQWDLQDPLT
                     FAQDGSFDLVLCPLVMDYIKEWQSVFQEFFRILRKGGIFIFSCGHPAFLFYRNFPEDN
                     YFDIVLHEMEWKGFGEPCPHIKSHRRSLQDILNSLINAGFQIDRILEPRPAPDFPRTA
                     ETEEIYQDLLREPCFLHIRAVK"
     gene            3036..3614
                     /locus_tag="PLL88_08325"
     CDS             3036..3614
                     /locus_tag="PLL88_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ01609.1"
                     /translation="MNRERTRKVVITGLMAAIACLLGATHWGFIPWIGGVSLTILQIP
                     VIIAAVLEGPLSGMMVGLIFGIFSMIQAAVAPTGPTDVWFTNPVLAVLPRLFIGPAAW
                     GVYTLLKRLPALGLLAAGAAGSLTNTVLVLGTIGLLKYLPWEALGAVVVTNGLPEMFA
                     AAVITLAVIGAYWQIPSKKKGSDLEDRTSGLS"
     gene            complement(3655..4488)
                     /locus_tag="PLL88_08330"
     CDS             complement(3655..4488)
                     /locus_tag="PLL88_08330"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOJ01610.1"
                     /translation="MPAFEYLRSIGIGYYYPGESFLHRRDARIKILFFGIFILSVTLT
                     NSLYRLLFGLLVACLGFWMAKIPILPCLRTMRTALPFILILLILQLILHNFNAGSDPL
                     WKWGIFTVYESALFSALRIVVRFFTLILVLGLMTAVLSTLEMNHGLEMLLRPLERLGV
                     RTDMLVMVVQIMLRFLPLLALRMEEIAKSQAARGAEWDSGKGGIRKRVKLALPLIIPL
                     FLHTLQQAERIADAMLCRGYGLHPVRSHYYEYKIGFADLLFLLIGLGISGIILYSPAI
                     S"
     gene            complement(4475..6226)
                     /locus_tag="PLL88_08335"
     CDS             complement(4475..6226)
                     /locus_tag="PLL88_08335"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOJ01611.1"
                     /translation="MKNFIELHHVHYSYPLPAGERVRAIQDVSFAIRPGERVALIGAN
                     GSGKTTLVKLINSLLLPDSGTVRVDGFSTSDRDRQMEIFNRIGLIFQNPRDQIVASLV
                     KEDTAFGPENLCLPRQEIRKRVNASLAMCGALHLKERQTYLLSAGETQRVALAGVLAL
                     RPACLIFDETTAMLDPLSRSELLDLISALHTDGFTIVQITHDLDETLAADRILVLSAG
                     RLVMDGTPQEVFKDKDTLRKYGLAVPFTIRFARDLATCFPEMADFYKSESELIKDLNT
                     VPHQEKKAEFLQGDSQDVPRNTYIKVQHLSHTYMADTPLARRTLQGVSFSIPERDSVG
                     LVGKTGSGKSTLLQHLNGLIRPQRGKVRVGEFDLTRTEIDIKTLRRQVGLVFQTPEAQ
                     FFERYVGDEVAFAARMLGYSGKLRDVVRDALQAVGLDFTQYVDRPLASLSGGQKRRVA
                     WASFLVVQPDVLLLDEPFAGLDPLTHQEMIGLVTRMSEEGKTILLSTHHMRDLLQITQ
                     KAFVLQDGKMPFEGHVTDLFRQPDLQNWGLGIPLELRVAEGLRLKGWTIPAGSVCWEE
                     ILAWVRSGMQGGGNACI"
     gene            complement(6315..6509)
                     /locus_tag="PLL88_08340"
     CDS             complement(6315..6509)
                     /locus_tag="PLL88_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01612.1"
                     /translation="MKTAVCPDCGSDINVGSQPRVGKQVVCDSCGAELEVVWLDPIEL
                     DYPMMDIDDLEEYSDEDDER"
     gene            6701..7627
                     /locus_tag="PLL88_08345"
     CDS             6701..7627
                     /locus_tag="PLL88_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01613.1"
                     /translation="MPSRTLQLRNFTPILDDLHMRFQLLAENTLAVQGITIAERNDLL
                     EDIRATRRTILKRCQSRACQPADLNDSGYRGYLWLVFLSESRRFDQHLKFIIRFLQEW
                     KKRTNKTVTVRMYNSPFVFQCKPEKNQTKVTIGEGFLTSDDQAIQILVDCCLQSTRRS
                     LAHIRKVSRSSTYKQVMDAIWQHGGQSTRSTKGDHYDLQQLFDRLNAEYFQGKLEQPR
                     LCWSSRRATRRLGYYHPDTDTITVSRYLDQSAIPLYVVEYVLYHEMLHKKLGLKEVNS
                     YRLAHTTQFKQQERKFKFYDEAEKFFKTFSRT"
     gene            complement(7650..9563)
                     /gene="feoB"
                     /locus_tag="PLL88_08350"
     CDS             complement(7650..9563)
                     /gene="feoB"
                     /locus_tag="PLL88_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOJ01614.1"
                     /translation="MRFALVGQPNCGKSTLFNQVAGYKAATGNFSGTTVTFTETKVHV
                     QGKVIELVDLPGTYSLLGTNPAERVVLDYLVSNMVDVIINVVDATHLAQGLTLTLELL
                     ELKRPTVLAINMMDEASRQGVHVDGEALSRFLQIPVLPIVATKGQGVRTVFSTAYTIG
                     KDQTNAPRQLYSPELEEQIDHLKNLIAPVRLPIGSEALALKLLEGDPALIDQVGKKIK
                     GAEAEIQKAVDTLCHITGRQAVWLVSKERHDLALQICEKVYKKTKKRLTLRDRLDDYL
                     LHPFFGYVFLIAILFLFFWSVNKVGGVIEAPLIQLSDYLDARLLELIGSEGLFAQILS
                     GALQGFMGGLAIVLPYLVPFLIGLGLLEDVGYLPRIAFLMDSLMHRMGLHGKAVVPFI
                     LGYGCNVTSIMSTRIMEEKRDRFLSALLSSMVPCSARMAIIFGLAAFYLGPVYGVGIY
                     ILNIVVIALTGRIISHYLPEDSPGLILEMPVYRFPTLRTVTAKAWLRIREFIVEAWPL
                     LIVGSVLLAVMNHYHLAQITDVIFRPVTWLLNLPPQVGTPLIFGILRKELSLIMLGQA
                     LGSMDFSQVLTQVQMISYTIFVVFYIPCLATLVAIRKELGVKAMGAITAITLLVAVLS
                     ALIVRLVATPILG"
     gene            complement(9560..9787)
                     /locus_tag="PLL88_08355"
     CDS             complement(9560..9787)
                     /locus_tag="PLL88_08355"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOJ01615.1"
                     /translation="MNLLQVKNDIWVRVIGFRGGWGMQRHFMQIGFLPGNTARIIRTA
                     PFHGPLLVEVDDREIVIGRKVARHILVEEIA"
     gene            9956..11047
                     /gene="trpS"
                     /locus_tag="PLL88_08360"
     CDS             9956..11047
                     /gene="trpS"
                     /locus_tag="PLL88_08360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOJ01616.1"
                     /translation="MQNIYDFSTGNETDSLKECIARSPILWNELERQPQKFRALTGER
                     TTGTLHIGHYFGSLKSRVTLQKWGVEMWLVLADYQAITDRISTEKLKESVFEIILDYL
                     AVGIQPEKTIIFCHSMIPALNQLMLPFLSVVSMSELQRNPTVKEEIAFSGTKSVSGLM
                     MTYPVHQAADILFCKGNVVPGGKDQLPHIELTRQIARRINTMYFNGEKFFPEPELLLS
                     APILLGTDGRKMGKSLHNAIDLKMTEDETAQLIRQAKTDSNRNITYDPINRPEVANLL
                     RLYSLCVGMEPEKIADQIGEQGAAGLKRNLTEVVNEYFRPIRERRNQLSADSSYVWSI
                     LKEGNSKANEIADRTLSDLRNAMHMQYYK"
BASE COUNT         3155 a         2853 c         2517 g         2569 t
ORIGIN      
        1 tcatttcgat cgtaacacat gcagagaaaa cgagcatgcc gatcccaacg atcagccaga
       61 gcgatctctt tgctcttcca ttcaccatca attctcctct tcttttcact taaatcatta
      121 tacaaaccgc aatgggaaaa acaacgatac gaacaggagc gttgaaagtc caggatttca
      181 tcagagaaat tagggaaaga agaaaaatgt agccacagga aatcctgtgg ctacgcgtga
      241 gaaattactt ttttttgtca aaaaggcgga aaacaagatt cagtgcaatt cccaacagcg
      301 ctccggtcgc gatcgccggc cagccatcag gaaagacgga tcgcagcaac ggaaccggca
      361 tcaacccgtt ggcatatccg atatgcccgc ccacgccgat gatgaaaatg accgctccga
      421 ttgccaggtt gcgcgggtca aacagatcga ctttctcttc aatcatcaaa gccacaccct
      481 gcattccaat cacaccgaac agatagattg acagaccacc tgtaacagcc gtgggaatgc
      541 tgcgcacgat gccactcagg atgccgctga aagaaagtat catggcaatg acagccgcac
      601 acaccagcgt ataacctgaa taattttggg ttatcaccat cagagaatta ttctcaccat
      661 aatttgtgcc tgctgctcca ccaataagcc cgttgatcaa atcgcccaga ccatccaaga
      721 caaggttgaa acctacatgg ttgctcagac cgaccttctg ccgccctttg ccttctgcca
      781 gtttatccac atacagcccg atttggtaca gatgggcggt agattcagga atggttgcaa
      841 tagccataat agcaatgctg gctatggcgg tcaaggagag atcgctggta aaggatggca
      901 gggtaatgtg gggaacctca aagaaattcg cttccgcaat cacagaaaag tccaccaatc
      961 ccagcgggat ggaaaccaca tatcccacga tcgcgcccaa caaaatgggc agcatgccaa
     1021 taaacccttt cccgcgcagg aatacggaaa agaaaatggt cgccagcagt gtgatgaacg
     1081 caaccagcca ccaggagccg ttatctacga taccaaaagc ccctccactg gcattttgca
     1141 gcgcagtgta gccaagcgag aacccgatca cgcaagcaac cgaaccggtg accacagggg
     1201 gcaaaatcct gttgatgaaa tttttgccac cacaataatg gaccagcaga ccgaccagaa
     1261 tattgataac gccagtgcaa acgatgccag cctgagcggt acggatcagc tcatcagaag
     1321 ccaccactcc atactgggct ttggtgatcg ccatgatggc agccagatag gaaaagcttg
     1381 aaccataata cagcggaatg aaagtgccta tcttgaattt ggataagatc aacgccacca
     1441 gggttgccat gcctgaagcc agcagcacgg tgttgatgtg aaaccccacc aggagcgcca
     1501 ccaacaccgt cgccgggaac atggtcagaa catgctgcaa acccaatagc agcatcctgc
     1561 caaacggcgg cttgtcgttg ggaagataat aattcttttt gagtacttcg ttcatttttt
     1621 tctcctttgg ataatttttt acaaaaaaag acccccgatt ttgggggtct ttttttatcg
     1681 aacattaatt accaacaggt gcgagaacag caagggatgg aattttaatt tcggatactt
     1741 ttttcgcatg cattctcctg taggcaaaac tgcttgaata taccatgaga tcttcttgct
     1801 gtaaaggtat agacaagttt caaccttaaa atttcctcca tcaaatctat agttttttat
     1861 acaaattcaa tgcggcatgc aacaaaacaa cggggggtgg aacgatcttt cggtagaaga
     1921 agaaactgat gcgaaagtga agctgccgga aagcgcgcgg attgatacgc tgaagcatgg
     1981 gcgccagccg gtaaagcaag ccataataca atttcataaa gaagagaaaa acgattgctc
     2041 caaagctcaa ctccatgatt tcctgtctta caattcgcag tggcagccgc caaaaaataa
     2101 atgtgaacgg tttcatcatg ctgcggaaag ggcgcagatc gttttttaat acttgaatag
     2161 ccattgaatg aattcctcaa taattatttt ttaatctacg cagtataacc ccaaagtggg
     2221 ataatagaat tgcacaaaaa gggatgatat gacaaagtca aatctggcaa aagagaacta
     2281 ttcctctttc gcagagcgtt acgctcaagg catcgctgcc aattcccata atgcttttta
     2341 tgaacgaccc gccacccttt ccctgcttcc ggaagtgaaa gatcttcgca tcttcgatgc
     2401 cgggtgcggt ccgggtattt acagccaatg gttaattgaa cacggcgccc acgtaactgc
     2461 tatcgacgca accccggaat ttgttcagat ggctcaagat cgcacccagc aaaaagcgaa
     2521 cgttctacaa tgggatctgc aagatccact tactttcgcc caggatggtt ctttcgatct
     2581 tgttctgtgt ccgctcgtta tggactatat caaagaatgg cagtcggtgt ttcaagaatt
     2641 tttcaggata ttgaggaagg gcggtatttt cattttctcc tgcggtcatc ctgccttcct
     2701 tttctaccgg aatttcccgg aagataacta ctttgacatc gtcctgcacg aaatggaatg
     2761 gaagggattt ggggagccct gcccgcacat caaaagccac cggcgttctt tacaggacat
     2821 actcaattca ctgatcaacg ctggttttca gattgatcgc atcctggaac ctcgcccggc
     2881 acctgacttt cctcgaacgg cggagacgga ggagatctac caagatttgc tgcgtgagcc
     2941 gtgttttctc cacatccgtg cggtgaaata gggcatttat cacttttttt ccaagcagcc
     3001 atgctacaat aaatgtctgt ctggagggac tgtttatgaa tcgtgaacga acacgaaaag
     3061 tcgtcattac cggtcttatg gctgccatag cctgtctgct cggggctacc cattggggtt
     3121 tcatcccatg gatcggagga gtatccctga ccatcttaca gattccggtg atcatcgctg
     3181 ccgtactgga aggaccgctt tccggaatga tggtggggtt gatctttgga atattcagca
     3241 tgatccaggc tgctgtggca cccacaggtc cgacggatgt gtggttcacc aatcccgtgc
     3301 tggcggtgct accgcgcctg ttcatcggac cggccgcctg gggcgtgtac accctgctta
     3361 aacgcttgcc ggctcttggg ttgctggctg ccggtgccgc aggaagcctt accaataccg
     3421 tcctggtgct gggcaccatc ggactgttga agtacctgcc atgggaagca ctgggagctg
     3481 tggtcgtgac caacggatta cccgaaatgt tcgcagcggc agtgatcacg ctggcagtga
     3541 tcggggctta ttggcaaatc ccgtcaaaaa agaagggttc tgacctggag gatagaacga
     3601 gcggtttatc atgaatcgct ctttttcctt tccatcccaa aacatcgctc gcaatcaact
     3661 gatagccggt gaatagagga ttattccaga tatccccaag ccgatcaaca aaaacaatag
     3721 atccgcaaat ccaattttgt attcgtaata atgacttctc acagggtgca gcccgtatcc
     3781 gcgacacagc atggcatctg caatacgctc cgcttgctgc agggtatgca gaaaaagcgg
     3841 gatgatcagc ggcaatgcca gtttaacacg cttgcggata cctccttttc ctgaatccca
     3901 ctccgctcca cgcgccgcct gcgatttggc aatctcttcc atgcgcaatg ccagcaatgg
     3961 caagaagcgc aacatgatct ggacaaccat caccagcata tccgtgcgaa cacccagcct
     4021 ttccaaaggg cgaagcaaca tttccaagcc atggttcatt tccagggtgg aaagaaccgc
     4081 agtcatcaag cccaaaacca ggatcagggt gaaaaagcgg acgactatgc gtaatgcaga
     4141 aaaaagtgcg ctttcataga cagtaaaaat tccccacttc cacaaagggt ccgatccagc
     4201 attgaaatta tgaaggatta attgaaggat caataaaatt aggatgaagg ggagtgcggt
     4261 gcgcatggtg cgcaggcaag gcaaaatcgg aatcttcgcc atccaaaagc caagacatgc
     4321 caccagtaag ccaaagagca atcgatagag agaattggtc aacgtcacag acaggatgaa
     4381 aatgccaaag aacaatattt ttattcgcgc atcgcgcctg tgcaaaaagg attcaccggg
     4441 atagtaatag ccgatgccaa tggaacgcag atactcaaat gcaggcattt ccacccccct
     4501 gcatacctga gcgcacccaa gccaatattt cttcccaaca cactgaacct gctgggatcg
     4561 tccacccttt taaacgcaac ccttctgcaa cgcgcagttc cagtggaatg cccaagcccc
     4621 agttttgcag atcgggttga cgaaacaaat ctgttacgtg cccctcaaag ggcatttttc
     4681 catcctgcag cacgaacgcc ttttgcgtga tctgcaacag atcgcgcata tgatgggtgc
     4741 tgaggagtat tgtcttccct tcttcgctca ttcgcgttac caaaccgatc atctcttgat
     4801 gggtaagggg gtccaatccg gcgaacggtt catccaaaag cagcacgtca ggctgcacca
     4861 ccaaaaaaga agcccatgcg accctgcgtt tctgcccacc gcttaaactc gccaaaggtc
     4921 ggtctacgta ttgggtaaaa tccaacccaa ctgcttgcaa ggcatctcgc accacatcac
     4981 gcaactttcc tgagtagccc aacatgcggg cagcaaaagc gacctcgtct ccaacgtacc
     5041 tttcaaaaaa ttgcgcctca ggagtttgaa aaaccaaacc cacctgccgg cgcagcgttt
     5101 ttatgtcaat ttccgtccgg gtcagatcaa attccccaac gcgcaccttt ccacgctggg
     5161 gtcgaatcaa cccattcaaa tgctgcaaaa gcgtggattt tccagatccg gttttgccca
     5221 ccagaccaac actgtctcgc tcggggatcg agaaagaaac accctgcaac gttctgcgcg
     5281 ccagcggggt atcagccatg tacgtatggg aaagatgttg gactttgata taagtgtttc
     5341 gaggtacatc ctgtgagtca ccctgcaaaa attcagcttt cttttcttga tgtggaactg
     5401 tgttgagatc ttttatcaac tctgattccg acttgtaaaa gtcagccatc tcagggaagc
     5461 aggttgccaa gtctcgcgca aaacggatcg tgaagggaac agccaacccg tattttcgta
     5521 aggtatcttt atccttgaac acttcctgcg gagtgccatc catgaccagg cgccctgcag
     5581 atagaacaag tatgcgatca gcagccagtg tttcatccag atcgtgagtg atttgaacga
     5641 tcgtgaaacc atccgtatgc agagcgctta tcaaatccaa cagttccgag cgggacaaag
     5701 ggtcgagcat ggcagtggtc tcatcaaata tcagacatgc cgggcgcaag gctagaacgc
     5761 cagccaaagc aacacgctgg gtctcccccg ccgacaataa ataggtctgc ctctctttca
     5821 ggtgcaacgc tccgcacata gccaggctgg cattcacccg tttacggatc tcctggcgcg
     5881 gcaaacataa gttctcggga ccaaaagcgg tatcttcttt taccagcgat gccacgatct
     5941 ggtcgcgcgg gttctggaag atcaagccaa tgcggttgaa tatttccatc tggcgatctc
     6001 ggtcagaagt ggaaaaacca tccaccctga ccgtgccaga atcaggcaaa agcaaactgt
     6061 tgatcaactt gaccagggta gttttccctg agccatttgc accgatcaga gcgacccgct
     6121 ctcccggtct gattgcaaaa gaaacatcct ggatcgctcg gacacgctct cctgcaggaa
     6181 gggggtagga ataatgaaca tgatgcaatt cgataaaatt tttcatcaac atccggcaac
     6241 ccattttaac attgaaaaaa ggcattgagc ttatggttca atgcccttct cttaagaaag
     6301 aattcgatga ttaattatcg ttcgtcgtct tcgtcagaat actcctctaa atcgtcaatg
     6361 tccatcattg gataatccaa ctcgataggg tccaaccaca ctacctcgag ttctgcgccg
     6421 caggaatcac aaaccacctg cttgccgaca cgcggctgcg aaccaacatt tatgtcactt
     6481 ccgcaatcgg ggcatactgc agttttcatc ttttcttgtc tccttttaga tgaatgagta
     6541 attttcatcc gtttatcaac ggaattattg cgggcaatat tacaccagat taaataaaat
     6601 gaaaagatgg caaaaatcga gaagaacttc gtgatttctt ctgaatcgca ttaaaatcag
     6661 catagcaagt accatatcag ctttcaggaa aatttgattt atgccatcaa gaacattaca
     6721 gttgcgcaat ttcacgccga tccttgatga tctgcacatg cgcttccagc ttctcgctga
     6781 aaacaccctc gccgtacagg gaatcacaat cgcagaaaga aatgatcttt tggaagacat
     6841 ccgtgccacc aggcgaacca tcctcaagcg ctgccagagc cgcgcctgcc agcccgccga
     6901 cctgaatgat tcaggatatc gaggatattt atggctggtt tttctaagcg aatcaaggcg
     6961 cttcgatcag catctcaaat tcatcatccg gttccttcaa gaatggaaaa agcgcactaa
     7021 caaaacagtg accgtgagaa tgtataacag cccttttgtg ttccaatgca aacctgaaaa
     7081 gaatcagacc aaggttacca ttggagaggg ttttttgacc tctgacgacc aagccatcca
     7141 gatactggta gattgctgtt tacaatcaac ccgcaggagc ctggcgcaca tccggaaagt
     7201 aagtcgatct tcaacctaca aacaggtcat ggatgccatc tggcaacatg gtggacaatc
     7261 gacacgttcc accaaggggg atcattacga tctacaacaa ctttttgaca ggctcaacgc
     7321 tgaatacttt caaggcaaac tggaacagcc gcgcttgtgt tggagcagca ggcgggcaac
     7381 tcgccgcctg ggttattatc atcccgacac cgataccatt acagtgagcc gctatcttga
     7441 tcaaagcgcc atccccctct atgttgtcga atatgtgcta tatcacgaga tgctgcataa
     7501 gaaattgggg ttgaaagaag tgaattcgta ccgcctggca cataccaccc aattcaagca
     7561 acaggaaaga aagttcaagt tctatgatga agcagaaaaa tttttcaaga cattctcaag
     7621 aacttgatat ggcggtggga atacatgccc taccccaaga tgggggttgc gaccaggcgc
     7681 acaatcaagg cggagagcac tgccaccagc aaggtgatcg cggtaatggc acccatagct
     7741 ttgacgccca attccttgcg gatggcaacc agggttgcca gacaagggat ataaaaaacc
     7801 acaaaaatgg tgtagctgat catttggact tgggtgagaa cctggctgaa atccatacta
     7861 cccagcgcct gacccagcat gatcaacgat aattccttgc gcagaatgcc aaagatgagc
     7921 ggtgtcccaa cctgcggtgg cagattcaac agccaggtaa ccggtcggaa gatcacatca
     7981 gtgatctgcg cgaggtgata gtgattcata acagccagca gaacactgcc cacgatcaac
     8041 agcggccagg cttctacaat gaattcccga atgcgcagcc aggcttttgc cgttacggtg
     8101 cgcagggttg gaaaacgata taccggcatt tccaggatca aaccaggtga atcttcggga
     8161 agataatgag aaatgatacg accggtgaga gcgatcacca cgatattcag aatataaatg
     8221 cctacaccgt aaaccggacc cagataaaaa gctgccagac caaagatgat cgccatgcgg
     8281 gctgaacagg gcaccatact ggaaagcaat gccgaaagaa agcggtcgcg cttttcttcc
     8341 atgatacggg ttgacataat tgaggttaca ttgcaaccat accccaggat gaaaggtacc
     8401 accgctttac cgtgcaaccc catgcggtgc ataagggaat ccatcaaaaa cgcgatgcgc
     8461 ggcagatacc ccacatcttc caggagaccc aatccaatca ggaacggtac aagataaggc
     8521 aacacgatcg ccagaccgcc catgaatccc tgcaacgctc ccgataggat ttgcgcgaac
     8581 aatccttcac tgcctatgag ttccaataac ctggcatcca aataatcaga aagttggatc
     8641 aacggtgctt caatgactcc accgaccttg ttcactgacc agaaaaagag gaacagaatg
     8701 gcaatcaaga acacatatcc aaagaacgga tgcagcagat agtcatccaa acgatcacgc
     8761 agcgtcaacc gttttttggt ttttttataa actttttcgc agatttgcaa agccaggtca
     8821 tggcgttctt tgctcaccag ccacaccgcc tgcctgccgg tgatatggca aagcgtatcc
     8881 accgcttttt gaatctccgc ttcagcaccc ttgattttct ttccaacctg atcgataagg
     8941 gcaggatctc cttctagcaa tttcagagcc aaagcttcac tgccaatcgg caaacggacc
     9001 ggcgctatca gatttttaag atggtcgatt tgctcttcca attcaggaga atagagctgg
     9061 cggggggcat tcgtttgatc tttcccgata gtgtacgcgg ttgaaaaaac cgtccgcaca
     9121 ccctgccctt tggtagcgac gatggggagc acaggaattt gtagaaacct gctcaatgcc
     9181 tccccatcaa catgtacacc ctgcctggaa gcctcatcca tcatgttgat tgccagcacc
     9241 gttgggcgct ttaactccag cagttccaaa gtgagggtta aaccctgcgc gagatgggtc
     9301 gcatccacca cattgatgat gacatccacc atattgctta ccagataatc taaaacaacc
     9361 cgttcagccg gattggtacc aagcagtgaa taggttccgg gaagatcaac cagctcgatc
     9421 accttaccct gcacgtgcac cttcgtctca gtgaacgtta cggttgtccc tgaaaaattg
     9481 ccagtggcgg ctttatatcc agccacctgg ttaaacaggg tgctttttcc gcagttgggc
     9541 tgccccacca gtgcaaatct cacgcaattt cctctaccaa aatatggcgc gcgacctttc
     9601 gtccgatcac gatctcacgg tcatctactt ctaccagaag cggtccgtga aagggtgcgg
     9661 ttcgtatgat cctggcggtg ttccccggca aaaaaccgat ttgcataaaa tggcgctgca
     9721 tcccccagcc gccacgaaac ccgataactc taacccagat gtcatttttt acttgtaata
     9781 aattcatgcg attagtatat gctaatgcac attttgcgtc aataaactaa aaatattgct
     9841 gcaaaaatgg taatcagaac tccgttacaa accacttctt gatcatgttg tacccagaca
     9901 gaacgccatg aatggaatat aatcttacaa aagacaagca cgggaaaaag attgcatgca
     9961 aaacatttac gatttcagca ccggtaacga gaccgattct ttaaaggagt gtatcgcgag
    10021 aagtccgata ttgtggaatg aactcgaacg acaaccccaa aaattccgtg cattaactgg
    10081 cgagcggact accggcaccc tccacatcgg tcactacttc ggatctttaa aaagcagggt
    10141 gaccctgcaa aagtggggag tagagatgtg gctggttttg gcagactatc aagccatcac
    10201 cgaccgaatt tcaaccgaga aactaaaaga aagcgttttt gagatcattc ttgactattt
    10261 agccgtaggc attcaaccag aaaagacaat cattttctgt catagcatga tccctgctct
    10321 caaccagctt atgcttccat ttctgagcgt tgtaagcatg tcagaactgc aacgcaatcc
    10381 cacggtaaaa gaagagatcg cgttctctgg cacgaaatcc gtcagcggtt tgatgatgac
    10441 ctatccggtt caccaggctg ctgacatcct tttctgtaag gggaacgtcg tgccgggtgg
    10501 aaaagatcaa ttaccgcata ttgaactcac acgccaaatt gcgcgccgaa taaacaccat
    10561 gtatttcaac ggagagaaat ttttccctga gccagaactg ttattgagtg cgccgatatt
    10621 gctgggaacc gacggtcgta aaatgggtaa gagcttgcac aatgccatcg atttgaaaat
    10681 gacagaggat gaaacagcgc aattaattcg acaagcaaaa accgactcga acaggaatat
    10741 cacgtatgac ccgattaatc gcccagaggt cgccaacttg ctacgactct attccttgtg
    10801 cgttggtatg gaacctgaga aaattgccga ccagatcggt gagcaaggag ccgcgggttt
    10861 aaaaagaaat ctcactgaag tggtgaatga gtattttcgc ccgattcgag aacgccgcaa
    10921 ccagttgagc gctgatagct catatgtatg gagcattttg aaagagggaa attcaaaagc
    10981 aaacgagatc gccgatagaa ctttaagcga tctcagaaat gccatgcata tgcagtacta
    11041 caagtaacaa actataactt cggcaacacg atgctctgtt gtgcctgata tttc
//
LOCUS       DAOSDS010000071        11068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2266_length_11068_cov_5.343231, whole genome shotgun sequence.
ACCESSION   DAOSDS010000071 DAOSDS010000000
VERSION     DAOSDS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11068
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2266_length_11068_cov_5.343231"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="PLL88_08365"
     CDS             <1..1281
                     /locus_tag="PLL88_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01617.1"
                     /translation="NLGLVFGCFSFYFFMVYLEQKNKRLLWLASLLLVLGLVTRAGPI
                     FIFPMIMVGVFLRDKSKKNFWQIFLISLLVVGVVIAAFVLLSSLVSPEDSIPFANFAH
                     SLYGLATGGRGWGAIQIDHPEVFDLQEPELTKAIFDYSIQAIKDNPGNLIKGALLQYP
                     QIINFPDRKGFFSFFRSENLLVYYLGQVALFVLFFYAIYSEMKNSSYKKYRMFFYALI
                     GILLSVPLLPFNDFKEMRVYAAAIPYILIFPCMGLAALLEKLPNSKLRSIKTASINST
                     TPVALSILLMACTLLGPIFVWKLTAKNRPPTTIECAKGEDAIVAFIAEGSSFSLLKES
                     DLYLDWLPYFHESRFRKNLHNLQDKIVMAFEDVHAPSLLTSTINLQNGDAVMLVFPNE
                     EQARFNETQYLCGVWDDYDYSTYNANLFFVRSHE"
     gene            1360..1881
                     /locus_tag="PLL88_08370"
     CDS             1360..1881
                     /locus_tag="PLL88_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01618.1"
                     /translation="MDENLDEKKCPVCGRPNLSQAEKCWYCQAPLENSKETRQSSLLS
                     EEDLLEQNGEGSSFVQSAMEEKTRAEPAAIPEWLHRVRELIAADRQQDEPTDDWQQQQ
                     LFDVPEKHESKHVKTTPRVANPPPAAATSKEAKATAGANKKEVAELPEAPDDLPEGFT
                     PISTQQNDKTSKG"
     gene            1881..2273
                     /locus_tag="PLL88_08375"
     CDS             1881..2273
                     /locus_tag="PLL88_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01619.1"
                     /translation="MMEHDRKDIEDQNGIAEEESILENPVPKKKKKKRHPMRWFLLLI
                     LCVVAFLYAQQWYLDLEAEALTYARQTAGVFESATAQSAMQKLLEATNTSTQPPPTAT
                     EDALLMYTQTVAAQLTNVAEFQQTPAAQ"
     gene            complement(2314..3774)
                     /locus_tag="PLL88_08380"
     CDS             complement(2314..3774)
                     /locus_tag="PLL88_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HOJ01620.1"
                     /translation="MPIHFGTDGWRAVISDTFTFHNLQLVSQAIADAVASEGWVNGTS
                     LETKPDPKKIVIGFDTRFLSDRYAKEAARVMAANGYRVFLCQSGAPTPTVSFAVRELN
                     AIAGIMITASHNAPRYNGVKLKACYGGSASPDQCRKVEIYLNDNEELGRGPNLVDFDK
                     ARADGLITRFNPLPDYYDHLRKLIDFNIIAENPQYIVVDPMYGSGRGVIKGVLEGTGC
                     EVYEIRGEMNPGFGGVHPEPIANNLKPLSGALINGKGTLGLATDGDADRIGAMDERGV
                     FVDPHRIMALALRYLVEKRYLTGAVVRTVSTTRMIDRLAKRYALTLYETPVGFNQIAE
                     HMLSEQVLIGGEESGGISFLGHIPEGDGVLMGLLLTEIVAESGSTLSELVEDLLADVG
                     PAFYERTDLRLNRPLAKQRMVDQLISDAPASIGGESVAQVVSLDGVKYIMADDSWLLI
                     RPSGTEPVLRVYAEGRTTAMVQALLRYGEKIAEKFQ"
     gene            3922..4914
                     /locus_tag="PLL88_08385"
     CDS             3922..4914
                     /locus_tag="PLL88_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01621.1"
                     /translation="MKKRTVTFLLLIVLSSLFTACKGKIQEAVTQETPTSLPTPTTAA
                     DRVVLITGENSDAWTLQQASSTLQELAAPSGLAFETRTAIQSNEITADMKVLVFLNHP
                     QNLGSFSNEAPHTQFIVLSDQDWSPSSNVTIIRTDPKNQFFMAGYASVMLAENFRSGG
                     LLVSETGDFSLAYRNGATYFCGICNAVITPLNQYPVIKELSQTSQPGDWIAAFDEISL
                     STILYIFVPPQAYSTELFNRIAQTNVKVIGISTPPAEAQPIWAGTFLIDGIAPIRNLW
                     NEVVAGNGGKTVNASLSLTDTQAGLISPGRQQLLLQVIEEMQAGHIYTLDLLAE"
     gene            complement(4916..5539)
                     /locus_tag="PLL88_08390"
     CDS             complement(4916..5539)
                     /locus_tag="PLL88_08390"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOJ01622.1"
                     /translation="MQCFSPDVPAEKRIFDILVSLLVLTVLSPLYLLIAISIRISEGA
                     PVIFAQERPGLQGKIFRVWKFRTMRVLKDEKGELLPDAERISPLGKFLRKTSLDELPE
                     FVNVLRGEMSLVGPRPLLVAYLARYSPAQARRHEVLPGITGWAQVNGRNAISWQKKFE
                     LDVWYVDHWTFWLDLKILFLTIWKALSGEGVSQPGHATMDEFMGDRK"
     gene            5605..5853
                     /locus_tag="PLL88_08395"
     CDS             5605..5853
                     /locus_tag="PLL88_08395"
                     /inference="COORDINATES: protein motif:HMM:NF021758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HOJ01623.1"
                     /translation="MIKKVSIEYCVPCQYEREAKNLAGIIQDQFGLAAESIELIPSKK
                     IGTFEVQVDDVPLYSKSKSGALPTPEEIIRLIFMQPRG"
     gene            5863..6849
                     /gene="trpS"
                     /locus_tag="PLL88_08400"
     CDS             5863..6849
                     /gene="trpS"
                     /locus_tag="PLL88_08400"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOJ01624.1"
                     /translation="MNKKGRVFSGARPTGRQHLGNYLGAVRNYVELQDDFSCIYCVVD
                     IHALTTMETTRELKDNTMEMALDWLAAGVRPAETMMFIQSHVPEVMELHTMLSMVTPL
                     GKLTDLPTFKEKVREQPNNVNYGLVGYPVLMAADILLYKANFVPVGVDQAPHIEFTRE
                     VARSFNFRYGKNVLVEPQMKVTEVPKVLGIDGQQKMGKSLNNHIEIAATPEETQARVM
                     QMVTDPARMKRSDPGNPDVCNVFSMHKIFSSKEEIDMVNVECRRAGIGCVDCKKLYAR
                     NLNAHLAPFRERRAELSNKPQDVWDILADGAKRARSIAQETMREVRDAVGLP"
     gene            6851..7288
                     /gene="dtd"
                     /locus_tag="PLL88_08405"
     CDS             6851..7288
                     /gene="dtd"
                     /locus_tag="PLL88_08405"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOJ01625.1"
                     /translation="MKAVLQRVSHSSVSVDDRLISSIGRGLLILLGIENDDTVEEAKI
                     LCKKIANLRIFEDELNKMNISALDIHGEMLVISQFTLMADTRKGNRPSFINAAPPAIA
                     QPLIDEFCNILRDYELTVQQGVFGAHMAVELLNDGPVTILLEV"
     gene            7431..8114
                     /locus_tag="PLL88_08410"
     CDS             7431..8114
                     /locus_tag="PLL88_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01626.1"
                     /translation="MKKNICSVCAVIVLLSIIFSACVRPASGTTDSVPTTNATLPNPV
                     STQSQLMKDIIAGTQTAMALTTSSGTPEPTSSSGTAASSTDTTSATDAPSATSTPALP
                     TATAGPPPVVTLTYNSKACGPGLYLCTVSVIKDQSVTLQATNSWLMKGWNLTFTMRPN
                     GTDASQGIVVGTAVYDTDGSPGFQVILTIPDALRSFETIIVRLDSKECTTYYGTECYG
                     QEYFYNTAQ"
     gene            8173..8703
                     /locus_tag="PLL88_08415"
     CDS             8173..8703
                     /locus_tag="PLL88_08415"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOJ01627.1"
                     /translation="MKIQEITPIHKGKIFSLETVNIAMPTGPIKTYERVNHPDSVSIL
                     PLDEDGNIWFVNQYRIGSEGELLELPAGVMEGGEDPLSSAQREIREEIGMAARSWQPI
                     GSLYLAPGYCTEINHIFLAEHLYSAPLSPDEDEYLTIVKIPCDEILNHLKKETIKDCK
                     SIAALGLFLLSPRLSV"
     gene            8787..9278
                     /locus_tag="PLL88_08420"
     CDS             8787..9278
                     /locus_tag="PLL88_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01628.1"
                     /translation="MSLINLCCGGKGNVLPGNHSYPTSLFSTRNNPNKIHWAENPLDY
                     TLHCNHHFEPCFQLRFCSPHDRFARIHRTTLMILICAEIGVILWQFRFALKTSLESLF
                     SKIRSALVNFISQWSNYIIPKHSDHEIMAEVTAKINTGDYRLLFEDANWIPYWFIKVI
                     NNT"
     gene            9449..>11068
                     /locus_tag="PLL88_08425"
     CDS             9449..>11068
                     /locus_tag="PLL88_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOJ01629.1"
                     /translation="MKRQYRMLDGNEAVAYVAYRLNEVIAIYPITPSSAMGEHADAWS
                     AARVPNIWGTVPTVMEMQSEGGAAGAVHGALTSGSLTTTFTASQGLLLMIPNMYKIAG
                     ELTPTVFHVSARSLATHALSIFGDQSDIAAVRSTGFALLASRSVQEAHDMAVIAQAAT
                     LESRIPFLHFFDGFRTSHEVNKVELLNDEDLKTMIDDDLIFEHRKRGLTPDRPSIRGT
                     AQNPDVFFQASETVNPFYDKCPDITQAAMDKFATLTGRSYHLFDYAGAPDARQVIVIM
                     ASGAETAEETVNYLTAKDEKVGIVTVHLYRPFSTKHFMAALPASVESIAVMDRSKDPG
                     ADGEPLYKDVTTAIYEGFATGIAPFKKVPIIVGGRYGLSSKEFTPGMVKGIFDELKKS
                     SPKNHFTVGINDDVSHSSLDYDEQFSIATDDVVRCVFFGLGSDGTVGANKNSTKIIGE
                     EGNNFAQGYFVYDSKKSGTVTESHLRFGPRPIKAPYLVGKGDANFVACHQFSFLEKYD
                     MLKYAKSGGVFLLNSIYGKDETWNNIPYEVQH"
BASE COUNT         2976 a         2890 c         2574 g         2628 t
ORIGIN      
        1 aaccttggat tggtttttgg ctgcttcagt ttctattttt tcatggtcta ccttgagcag
       61 aaaaacaagc ggttgttatg gctggcaagc cttttgctcg ttttggggtt ggtcacaaga
      121 gccggaccga tcttcatctt cccaatgatt atggtgggtg tctttttaag agataagagc
      181 aagaagaact tttggcagat tttcctgatt tccttgttgg tggtcggcgt tgtcatcgca
      241 gcctttgtgt tgctttcatc gctcgtcagc ccagaagata gcatcccttt cgcgaatttc
      301 gctcattctc tgtatggatt ggcaactgga ggcagaggct ggggagcgat acaaattgac
      361 catcctgagg tttttgacct gcaggaaccc gaattaacaa aggcgatttt cgactattct
      421 attcaggcaa tcaaggataa tccgggcaat ctcataaaag gcgcgctact gcaatatccg
      481 caaataatca attttccgga ccggaaaggc tttttcagtt ttttcagaag tgaaaacctg
      541 cttgtctact atcttgggca ggttgcactc ttcgtgcttt ttttctatgc catctatagt
      601 gaaatgaaaa actcttccta taagaaatat cgcatgttct tttacgcgtt gattggaatt
      661 ctcctcagcg ttcctttgtt accattcaat gatttcaaag aaatgcgcgt atatgccgca
      721 gccattcctt atatccttat ttttccgtgt atggggcttg ccgccctgct ggaaaaactg
      781 ccaaattcga aattgagatc aatcaaaacc gcatcaatca actctacaac tccagttgca
      841 ttgtctattt tattaatggc atgtactttg cttggtccta tattcgtttg gaaactcact
      901 gcgaaaaata gaccgccaac caccattgaa tgcgcaaagg gagaagacgc catcgtagcg
      961 ttcatcgcag agggatcttc tttctcttta ttgaaagaat ccgatttata ccttgattgg
     1021 cttccttatt ttcacgaaag cagattcagg aagaaccttc ataacttaca ggataagatc
     1081 gttatggcat ttgaagacgt tcatgcgcct tcattactaa ccagcactat caatctccag
     1141 aatggagatg ccgttatgct cgtcttccca aatgaagagc aagcgcggtt caatgaaacg
     1201 cagtatttat gcggcgtatg ggatgattat gactattcca cgtacaatgc gaacttgttt
     1261 tttgtgcgca gccatgaata aatccgcaag aattatcatt tcctgaaagt gctatcggta
     1321 tttgtgctat actctaaaaa acagagcctg agccaaccta tggacgagaa tcttgacgag
     1381 aagaaatgcc ctgtttgtgg aaggcctaac ctgagccagg cagaaaaatg ctggtactgc
     1441 caggcaccac tcgaaaactc taaagaaacc cggcagagca gtttgctatc agaagaggac
     1501 ctgcttgagc agaacggcga gggttcatcc ttcgtccaat ctgccatgga agaaaaaacc
     1561 agggcagagc ccgccgccat ccccgagtgg ttgcacaggg tgcgcgagct gatcgcagcc
     1621 gacaggcaac aggacgagcc gacagacgat tggcaacagc aacaattgtt cgatgttcct
     1681 gagaaacatg agtccaagca tgtaaaaacg acgccacggg tagccaatcc accccctgcg
     1741 gctgcaacct caaaagaagc caaagcgacc gctggggcaa acaaaaagga agttgccgaa
     1801 ttacctgaag ccccagatga cctgcccgaa gggttcacgc ccatttccac acagcagaac
     1861 gataaaacaa gcaaagggtg atgatggaac acgacagaaa agatatcgaa gatcaaaacg
     1921 gcatcgctga agaagaaagc attctggaaa atccggtgcc gaaaaagaaa aaaaagaaac
     1981 gccatccgat gcgctggttc ttgctcctga tcctttgcgt tgttgcattt ctgtatgcgc
     2041 aacaatggta tctggacctg gaagcggaag ccctgaccta cgcccggcaa accgccggcg
     2101 tttttgagag cgccactgcc caatcagcca tgcagaagct gcttgaagcc accaatacgt
     2161 caacccaacc tcctccaacc gccacagaag atgcgctctt gatgtacacg caaacggtgg
     2221 ctgcccagct caccaacgtg gcggaattcc agcagacccc cgctgctcaa taaaaacaat
     2281 cccttccatt ttttggaaag gattttcatt tatttactga aatttctctg cgatcttctc
     2341 cccatagcgc agcaacgcct gcaccatagc agttgtgcgc ccttcggcat acacccgcag
     2401 aaccggctcc gttccagacg gtcggatcaa taaccaggaa tcatctgcca tgatgtattt
     2461 caccccatcc agcgatacaa cctgggcaac gctctccccc ccgatcgagg caggcgcatc
     2521 ggatataagc tgatcgacca tccgctgttt ggcaagcggt cgattcagcc gcagatcggt
     2581 gcgctcgtaa aaagcaggac caacatccgc gagcaagtct tccaccagct cagagagcgt
     2641 gctacccgat tcggcaacga tctcggtaag cagcagaccc atcaacacgc catcgccttc
     2701 ggggatatgc cccaaaaagg aaatgccgcc cgattcttcg ccgccgatca acacttgctc
     2761 gctcagcata tgctcagcga tctgattgaa cccaaccgga gtctcataca gggtaagagc
     2821 atatcttttt gcaagccgat cgatcatgcg ggtggtagaa accgtgcgca ccacggcgcc
     2881 ggtcaaatag cgtttctcaa ccagatagcg caacgccagc gccatgatgc gatgcggatc
     2941 cacaaacaca ccgcgttcat ccatcgcgcc aatgcggtca gcatctccat ccgtcgccaa
     3001 ccccagcgtc ccttttccgt tgatcagcgc accagagagc ggtttcaggt tatttgcgat
     3061 cggttcaggg tgcacgccac caaagcccgg attcatctct ccgcggatct catagacttc
     3121 gcagcccgtg ccttctaaaa ctcctttaat aactcctctg cctgagccat acatggggtc
     3181 caccacaatg tattgcgggt tctcagctat gatgttgaaa tcgatcagct ttcttagatg
     3241 atcgtagtaa tcaggcaagg gattgaaacg cgtgatcaag ccatcagccc gcgctttgtc
     3301 gaaatcgaca aggttaggtc ccctgcccaa ctcctcattg tcgttcaggt agatctccac
     3361 tttacgacat tggtcaggcg aagcggaacc tccataacaa gccttcagtt tcacgccatt
     3421 gtaacgggga gcgttgtgag aagcggtgat catgatccct gcaatggcat tcagctcacg
     3481 gacagcaaaa gagaccgtgg gagtgggggc accagattgg catagaaaga ctctgtagcc
     3541 attggcagcc atcacacgcg ctgcttcttt tgcatagcga tcggatagaa aacgcgtatc
     3601 aaagccaatg acgatctttt tgggatccgg ttttgtttcc aaactggttc cattgaccca
     3661 tccttctgac gcgaccgcat cggcaatcgc ctggctgacc aactgaaggt tatgaaaggt
     3721 aaatgtgtct gaaattaccg cgcgccaccc atctgttcca aagtgaatcg gcatttcctt
     3781 tactcctgta acgataagtt tcaattaaaa agtccgcctg agaaattctc acagcgggct
     3841 gcatattcat tccatgtttc aaattgcgga tttcacgcga atattataac ttaatctccg
     3901 tcatggtgta caatgttgcc tatgaaaaaa agaaccgtca cttttctgct gcttatcgtt
     3961 ctcagcagtc ttttcactgc ttgcaaaggg aaaattcaag aagccgtcac ccaagaaaca
     4021 cccaccagcc ttcccacacc cacaaccgca gcagaccgtg tggttttgat cacaggcgaa
     4081 aactctgacg cctggacctt gcagcaagca agcagcaccc tgcaagagct agccgcacca
     4141 tccgggctcg cttttgaaac acggacagcc atccaatcca acgagatcac cgcggatatg
     4201 aaagtgctgg tctttctaaa tcaccctcag aacctgggca gcttttcgaa cgaagcgccg
     4261 cacacgcaat tcatcgtgct cagcgatcag gattggagcc cctcctcgaa cgtgaccatc
     4321 atccgcacag atccgaaaaa tcagtttttc atggcaggct acgcttcggt catgctggca
     4381 gagaatttcc gcagcggggg gctactggtc agtgagaccg gcgatttttc gctggcatac
     4441 cgaaacggcg cgacctattt ttgcggaata tgcaacgcgg tgatcacacc cctcaatcag
     4501 tatccggtca tcaaagagct atcccaaacc agccaacccg gcgattggat agctgctttc
     4561 gacgaaataa gtttgagcac catcctttat attttcgtgc cgccccaggc atattcaacc
     4621 gagctgttca accgcatcgc gcaaaccaac gtaaaggtca tcggcatctc cacccctccc
     4681 gccgaagcgc agcctatttg ggcgggcaca tttctcatcg atggaatagc acccatacgc
     4741 aatttgtgga acgaggtagt agccggcaat ggtgggaaaa cggtgaatgc cagcttatcc
     4801 ttgaccgata cccaggcggg gctcatcagc cccggcaggc aacagcttct gctacaagtg
     4861 atcgaagaga tgcaagccgg tcacatctat acacttgatc ttttggcaga gtgatctatt
     4921 tgcggtcgcc catgaactca tccatagtag catgccctgg ctgagaaacc ccctcaccgc
     4981 tgagtgcttt ccatatggtc agaaagagaa tctttaagtc cagccagaac gtccaatgat
     5041 ccacatacca gacatccagc tcaaattttt tctgccatga aatggcattc cgtccattga
     5101 cctgcgccca gccggtgatg ccgggcagca cttcgtgccg gcgagcctgc gccggggagt
     5161 agcgtgcaag ataagcgacc aacagcggtc gcggtccaac caaactcatc tcaccacgaa
     5221 gaacgttgac gaattcaggc aattcatcca aactggtctt gcgcaaaaat tttcccaacg
     5281 gcgaaatgcg ctccgcatcg ggcagcagtt ctcctttttc atcctttaga acacgcatgg
     5341 tgcggaactt ccacacgcga aagatctttc cctgcaaacc cggtcgttct tgtgcgaaga
     5401 tcaccggcgc gccttcgctg attcttatcg agatagcaat aagcaggtaa aggggagaca
     5461 agacggtcaa caccaatagc gataccagga tgtcgaaaat ccttttttct gccggaacgt
     5521 caggagagaa gcattgcata acctgatttt accaaaagca cgacgcgttt acgttataat
     5581 gaaaggaacc agccaggaga gacgatgatc aaaaaagtgt ccatcgaata ttgcgttccg
     5641 tgccagtatg agcgagaagc caagaatctg gcaggcatca ttcaggatca attcggattg
     5701 gcggcagaaa gcatcgaact gatcccatcg aagaagatcg gcacttttga agtgcaggtg
     5761 gatgatgtgc cgctgtattc gaaaagcaag agcggtgcat tgccaacgcc cgaagagatc
     5821 attcgtctca tatttatgca accacgagga taagaaatca tcatgaataa aaaagggcga
     5881 gttttttcag gagccagacc aaccgggcga cagcatctgg ggaattacct gggagcggta
     5941 aggaattatg tggagctcca ggatgatttt tcctgcattt attgcgtcgt cgatatccat
     6001 gcattaacca ccatggaaac caccagagaa ctaaaggaca acaccatgga aatggctttg
     6061 gattggctgg ctgctggagt gcgccccgct gaaacgatga tgttcatcca atcgcacgtg
     6121 cccgaagtga tggaattgca caccatgctc tcaatggtca ccccgctcgg aaagctcacc
     6181 gatctaccga cgttcaaaga aaaagtcaga gaacagccca acaatgtcaa ttacggtctg
     6241 gtcggctacc cggttctcat ggcagccgat atcttgctct ataaggcgaa cttcgtgccg
     6301 gtgggggttg accaggcgcc tcacatcgaa ttcacgcgcg aggtcgcgcg ttcattcaat
     6361 ttccgctatg gaaaaaatgt gctggtggaa ccgcagatga aggtcacgga agtccccaaa
     6421 gttttaggca tcgatggtca acagaaaatg ggaaaaagcc tgaacaacca tatcgagatc
     6481 gccgccacac cagaagaaac ccaggcgcgt gttatgcaaa tggtaacgga tccggcgcgc
     6541 atgaaacgca gcgacccggg aaacccggat gtctgcaacg tgttcagcat gcacaagatc
     6601 ttctcatcga aagaagaaat cgacatggtc aacgtagaat gccggagagc cggcatcggc
     6661 tgcgtggatt gcaagaaact atatgccaga aatctcaatg ctcacctggc tcctttccgc
     6721 gaacggcggg ctgaattatc aaataagccc caagacgtct gggatatctt agccgatggc
     6781 gcaaaacgcg caaggagcat cgcgcaagaa accatgcgcg aagtgcggga cgcggtcgga
     6841 ttgccctgat atgaaagcag tgctgcagcg cgtttcgcat agctcggtca gcgtagatga
     6901 tcgactgata tcctccattg gaaggggtct gttgatcctg ctcggaatcg aaaacgatga
     6961 cacggtcgaa gaggcgaaaa tcctctgcaa aaagatcgct aacctgcgaa tattcgaaga
     7021 tgagctgaat aaaatgaaca tttcagcgct cgacattcac ggcgagatgc tggtgatctc
     7081 gcagttcact ttaatggcgg acacccgaaa gggaaaccgc ccgtcgttca tcaacgcagc
     7141 ccctccagcg atagcccaac cgttgatcga tgaattctgt aacatattgc gcgactatga
     7201 gctcactgtt cagcagggag tgtttggagc ccatatggca gtggagttgc tcaatgacgg
     7261 accggtaacg atcctccttg aggtctagca ccgtcagcct ttgttctcat tcctgcagga
     7321 agatttcgag cgaaatcttc cttgacgtag gttctttggc gtgctaacat acattttaga
     7381 aattgaaaaa tattttctat tttcggaggg aagaacaaga ggagacatcc atgaagaaaa
     7441 atatctgttc cgtatgcgca gtaattgttt tattatccat cattttttct gcatgcgttc
     7501 gccccgccag tggaaccacc gatagcgttc caaccacgaa tgcaaccctg cccaatccgg
     7561 tgtcgacaca atctcaatta atgaaagata ttattgccgg aactcaaaca gctatggcgt
     7621 tgacgaccag cagcggcaca cctgaaccga cctcatcctc aggtacagcc gccagcagca
     7681 cagacaccac ctcagccacc gatgctcctt ctgccacctc cacgcctgcc ctgccgaccg
     7741 ctactgccgg tccaccgcct gtggtgacgt tgacctacaa tagcaaagct tgcggtccag
     7801 gtctgtacct ctgcacggtc agtgtcatca aagaccagtc ggtaaccctt caggcgacca
     7861 atagttggtt gatgaagggc tggaacctga ccttcaccat gcgaccaaac gggaccgacg
     7921 catcacaggg aattgtcgtt ggcacagccg tttatgatac agatggcagc cccggcttcc
     7981 aggttattct caccattccc gacgctttgc gcagttttga gaccatcatc gtacggctgg
     8041 attccaagga atgcaccacg tattatggaa ccgagtgcta cgggcaggaa tatttctaca
     8101 acacagccca atagctatcc aaaagaccgc gggaaagaac cgcggtcttt ttatttaatt
     8161 tacaatagaa ccatgaaaat acaagagatt acccccattc ataagggaaa aattttttcc
     8221 cttgaaacag taaatatcgc aatgcccaca ggtccaatca agacctatga acgggtaaac
     8281 caccctgatt cggtcagcat attaccctta gacgaagatg ggaacatctg gttcgtgaac
     8341 cagtatcgca tcggaagcga gggtgaattg ctggagcttc ccgcaggtgt gatggaaggc
     8401 ggagaagacc ctttatcaag cgcccaacgg gagatacggg aagagatcgg tatggcagcc
     8461 aggtcctggc agccgattgg ctcgctgtat ctggcgccag gatactgcac ggagatcaac
     8521 catatctttc tcgccgagca cctgtattcc gctcccctgt caccagatga ggatgagtat
     8581 ttgaccatcg tcaagatacc gtgcgatgaa atcctgaatc atttaaaaaa ggagacgata
     8641 aaagattgta agtcgatagc agcgttaggg ttattcctcc tgtcacctcg tttgagcgta
     8701 taaattcagc cacgggtgtt ttgagggata tcagcccttt aaattcgtcg aatggcacta
     8761 tttgcttttt tacgaatttt ctgatagtgt ctttaataaa tttatgctgt gggggaaagg
     8821 gcaatgttct tcctgggaat catagctatc caacaagtct tttttctacc cggaataatc
     8881 ctaacaaaat tcattgggct gaaaacccgc ttgattacac attgcattgc aatcatcact
     8941 tcgagccttg ttttcaatta cgtttttgtt ctcctcatga ccgctttgca cgtatacacc
     9001 gaaccacctt aatgattcta atttgtgcgg aaatcggtgt gattttatgg caattcagat
     9061 tcgcattaaa aacatctctg gagtcattgt tttcaaaaat acgcagtgca ttggttaact
     9121 tcatcagcca atggagcaac tatatcattc ccaaacattc cgaccatgaa attatggcag
     9181 aagttacggc aaaaattaat actggtgatt atcgcttatt gtttgaagac gcaaactgga
     9241 tcccctactg gttcattaaa gtaatcaata acacatgatt tttcttcact tttgttccaa
     9301 tactttaaaa tcgtaaaatc aactgaggat gcattatata gaaaagtggt atgattaatc
     9361 ggatttggga aaacattgat agaaagattc tttaccattt cttacctgct caagagccct
     9421 aataccattt acttaaggag tgtctttcat gaagcgtcaa taccgtatgc tggatggtaa
     9481 tgaggcggta gcttacgttg cctatcgatt gaacgaggtg atcgccatct atccaattac
     9541 accttcgtca gccatgggcg aacacgccga tgcctggtca gccgcccgcg ttcccaatat
     9601 ttggggaacc gtgcccacgg tgatggaaat gcaatcagaa gggggcgctg ccggcgcagt
     9661 gcacggtgcg ctcacttcag ggtctttgac cacgacgttc accgcatcgc aaggattgtt
     9721 gttgatgatc ccgaacatgt acaaaattgc cggtgagctt accccaaccg ttttccacgt
     9781 ttctgccagg tcactggcaa cacacgccct atcgatcttt ggcgatcaaa gcgatatcgc
     9841 agcggtgcgc agcaccggct tcgccttgct ggcatcgcgc tcagtgcaag aagcacatga
     9901 tatggctgtc attgcccagg ctgcaacgtt agaatcgaga attcctttcc tgcatttctt
     9961 tgacggcttc cgcacgtcgc atgaggtcaa taaggttgaa ttactcaacg atgaagacct
    10021 gaaaaccatg atcgatgacg atctgatttt cgagcaccgc aaacgcgggc ttacgcccga
    10081 ccgcccttcc attcgcggca cagcgcaaaa cccagacgtg tttttccagg caagtgaaac
    10141 cgtcaatcca ttctacgaca aatgcccaga catcactcag gctgccatgg acaaattcgc
    10201 caccctcacc ggtcgcagct atcacctctt cgattatgct ggcgctccgg atgccaggca
    10261 ggtgatcgtc atcatggcat cgggtgctga aacggcagaa gaaaccgtca actatctgac
    10321 ggcaaaagac gaaaaggtcg gcatagttac cgttcatctc tatcgaccat tctcgaccaa
    10381 acatttcatg gcagctcttc ctgccagcgt cgaatccatt gctgttatgg atcgctcaaa
    10441 agatccaggg gcagatggag aaccgctgta caaagatgta accacagcca tttacgaggg
    10501 ctttgctacg ggcatcgccc ccttcaagaa agttccgatc atcgtaggag ggcgctacgg
    10561 tctttcttcc aaagaattca cccctggcat ggtcaagggg atctttgacg agttgaagaa
    10621 aagctctccg aaaaatcact tcaccgtcgg catcaatgac gatgtcagcc acagttccct
    10681 ggattatgac gaacaattct ccatcgccac tgacgacgtt gtgcgctgcg tgttctttgg
    10741 actgggttca gacggaaccg taggcgccaa taagaattcc accaagatta tcggcgaaga
    10801 gggcaacaat ttcgcccagg gttatttcgt atatgattcg aaaaaatcag gaacggtcac
    10861 cgaatcacac ctgcgctttg gtcctcgccc cattaaagct ccttacctgg ttggaaaagg
    10921 cgacgcaaac ttcgtcgcat gtcatcaatt cagtttcctc gaaaaatatg acatgctgaa
    10981 atatgccaaa tccggcggtg ttttcttgct gaacagcatc tatggaaaag acgaaacctg
    11041 gaataatatt ccgtatgaag tccagcac
//
LOCUS       DAOSDS010000072        10841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2325_length_10841_cov_4.343686, whole genome shotgun sequence.
ACCESSION   DAOSDS010000072 DAOSDS010000000
VERSION     DAOSDS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10841
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2325_length_10841_cov_4.343686"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="PLL88_08430"
     CDS             complement(<1..1019)
                     /locus_tag="PLL88_08430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ01630.1"
                     /translation="MTITLKEVTSRRTLRKFVNFPFRLYKKNPYWVPGALRGDEIHTL
                     RWDKNPAFAFCKARYWLAYKNGKIVGRIAGIINPRYQERWGNAYARFGWIDFIDDPQV
                     SQCLLSKVEEWAKEQGKTAVHGPLGFTDLDPEAMLVEGFEEAGTLVGIYNYPYYQALL
                     ESAGYKKDIDWVEFEIKVPAGRVEKIKNLAKVVAERYHLHYLEPKNKKELLQYAHQLF
                     DLLEESYRHLYGVTPLSQEQVNAYISQYFGFISPEFIPIVLDETNKMVAFGIAMPSLS
                     QALRQARGRLVPLGFLYLLHALRKNNKGDLYLIAIRKDYQGKGVNSMLMARLLDVFAD
                     MGITSV"
     gene            complement(1144..2268)
                     /locus_tag="PLL88_08435"
     CDS             complement(1144..2268)
                     /locus_tag="PLL88_08435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ01631.1"
                     /translation="MSIEIKEITSLRTLKQFVNFSYQLYAGNPYWVPAIRSNVLKTLH
                     WEQNPAFEYCKAKYWLAYKGGKIAGRIAGIINPRFEERFKRRYARLGWFDFIDDYEVS
                     GALLNTVEDWARAEKQDAIHGPLGFTNLDPEAMLVEGFNEVATLAASYHYPYYKDHMQ
                     RAGYGKDTDYVEFEIKVPHKKVEKISKLAEIVAKRSNLHLLDAKNRKELLQYASQLFE
                     LIEEAYLHYGVTPLTRKQVDAYIDQYLGIIPPKFVPIVLDENNRMVAFGIAMPSLSKA
                     MQKNKGKLFPFGFLPVLRALRNNDKADLYLIAIRPAYQGRGVNAMLMAAMLDVFHEMG
                     IKSVESNPELEDNYRVQSQWKHFETRQHKRRRIFIKKFNH"
     gene            complement(2496..2858)
                     /locus_tag="PLL88_08440"
     CDS             complement(2496..2858)
                     /locus_tag="PLL88_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01632.1"
                     /translation="MPYEKETRRELPFPLEAVYQSAVHCIQHMGGKVIKQDAERGILH
                     AQMDKKLFGAYLGDRSQLEIQFTEIENGGTSIYIFAFPLNAIGQKLMFGARQGVVETM
                     MNAFYQELEKHLQNTQAS"
     gene            complement(2864..3793)
                     /gene="pfkB"
                     /locus_tag="PLL88_08445"
     CDS             complement(2864..3793)
                     /gene="pfkB"
                     /locus_tag="PLL88_08445"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HOJ01633.1"
                     /translation="MIYTVTLNPALDKEYRVPELLPNKILRASSVKIDFGGKGFNIAR
                     MLSTFDADCAALGFIGGHTGSILREGLKSNGIKTDFVEISGETRTNISAVSDKDRSYV
                     KINEAGPEILPEEIEQLLRKVEELIHPEDWWIMSGSLPRGVPHDIYAQMIQRINRGKA
                     RAILDSSGEALNYGCMAKPFLIKPNVEELTHITDLNVQDALALEEALSRIHAMGVKNI
                     ILSAGKEKAICSNGNQRWVGIPPKVDEINPIGAGDAMLAAIVYRLSREDSLPEAFRWG
                     LAAGSVAASLPGTMMPGLSDVEKLLEKAVVCEE"
     gene            complement(3894..5648)
                     /locus_tag="PLL88_08450"
     CDS             complement(3894..5648)
                     /locus_tag="PLL88_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HOJ01634.1"
                     /translation="MKNSKFAEYQNDLKIPESTLTWNLYGAGVENIGREGKPESFKIQ
                     PPDENQLLVRVDAVGLCFSDVKLIKLGGEHPKLSGRDLSAEPTRVGHEVALTVIKVGK
                     NLADEYQPGQRLAIQPDIYVNQHNTAYGYTIPGGLTQYHLMGAEVLDADDGAYVIPVD
                     NRVGYAEAALTEPWACVDAAYTQRRRLNPKTGGTMWIVGNKDDTTQYAFSEGLGAPKT
                     IFLTNIPDKFKEIIKKNVATETHIIETKEIYLEEIADFSARSTNGEGFDDIILLSPTS
                     GKFVEEIAKFIAFRGVLNIVGNKELDGDSLIDAGRIHYHYTAYVGTRSSDISAAYGEE
                     RNRCELRKNGTALFIGAGGPMGQMHVQRAIEKADGPAIVIATELNKERAEVLKKIIEP
                     LAMKKSKVFKIFNSNETEQSLQDFIFEISGDRCVDDAVICTPVAKLMEDTAKILKPDG
                     MLILFAGVPTGTLINMNLSSVFLGNLQLTGTSGSKLHDQKVVLQKTLRGELDPNRSVA
                     AIGGMQAAHEGLEALINGTFAGKIIIFPQINELPLTGLQDLENSYPQIAEKLGENYLW
                     TIEAEKALIEELWRGSAQ"
     gene            complement(5749..6015)
                     /locus_tag="PLL88_08455"
     CDS             complement(5749..6015)
                     /locus_tag="PLL88_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOJ01635.1"
                     /translation="MKKLNISLSNPTGLHARPAKNFVNLVKDYAAEISVFYNGKKANG
                     KSLISLLTLGAESGANILVEINGEDEETVFATLKECIDKGLLEE"
     gene            complement(6047..6493)
                     /locus_tag="PLL88_08460"
     CDS             complement(6047..6493)
                     /locus_tag="PLL88_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ01636.1"
                     /translation="MSILSKDCIRLNVKAKDKQDAICQSGKLLVEAGCVSPNYVDGML
                     AREEIMSTYLGNGVAIPHGQFDNRNDIFNSCISVLQIPEGVEWDEGEQVYLVIGIAAL
                     RNEHVTILANLAEAIDDEEIAQKMRTTRNVNVILDHLNKENKSSTE"
     gene            complement(6497..6826)
                     /locus_tag="PLL88_08465"
     CDS             complement(6497..6826)
                     /locus_tag="PLL88_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01637.1"
                     /translation="MAKSISTSEIKKILFACEAGMGSSLMSVNSLKKKMKKANIEGVE
                     VFHLATRDIPGDAQVVIVHRGLAKVACAKAPNAVILAFNQFLNDPIFDKIISAFVRKE
                     DITSNVE"
     gene            complement(6905..7969)
                     /locus_tag="PLL88_08470"
     CDS             complement(6905..7969)
                     /locus_tag="PLL88_08470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HOJ01638.1"
                     /translation="MKEKIQRLGGSMAGMIIPNIGAFIAWGLITALFIATGWIPNEKF
                     AANVSPILTYLLPILIGYTGGKMVHGTRGGVVGAIATMGVVVGSGVPMFLGAMIMGPL
                     GGWLIKKIDDVIEDKIPVGFEMLINNFTAGILGLLLVLLGNVLIGPVVESVSNWAGGV
                     VDSMINAKILPLVALIIEPAKVLFMNNALNHGVLAPLGVAAVEQSGRAIHFLLETNPG
                     PGLGLLLAYYVAGKGLLKESAPGAMIIHFLGGIHEIYFPYVLAHPIMILSVIAGGLAA
                     NLWFTIMNAGLVATPSPGSIFAYLAVIPKGQHFAVLVGVLIGAVASFLVGTLILKANP
                     LPEEKQEEAEETTKVPGLSK"
     gene            complement(8050..9594)
                     /locus_tag="PLL88_08475"
     CDS             complement(8050..9594)
                     /locus_tag="PLL88_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01639.1"
                     /translation="MVSLTTRQRDILRVLLKTNNPIRTYDLAMEIQLTPRQVHYSMKG
                     VKGWLTTKRVTLQTTPGRGIKLVCLPEQRVSLATELERDEGLQLVLAPEQRQQLICFL
                     LLIKAEPVLLTQLSFYLKVSRSTIVEDLDKVEKWLDERGISLTRKQNYGIHITSTEKN
                     KQQALLALLWGKCPFYPSVFGVSFQEGLTFTLAEDIQNTPLFKEINDFLCLLNMKRIF
                     NKVIFIEDSLGGRFTDEAVLYLALVFSVLSARIKTGNHIQVTDQELTDFLKMQEWKAA
                     EKMIKNLGQGNISDWQPGDIAYVVMYILSSPLLENWIGDVDKTGGYKDLFEELLEAIN
                     SLYKIDGLKDDPTLRDGIINYLIPIFSQHSYNLWFPKATVDLSFAFKFDDDLFLADQL
                     VSIIHTHTSITLDREDEYMLVAFIRAAYIRLRSYQFSNVLLVCPSGMATAQLLTARLK
                     TRFPHLGKLSVVSFRELNSERVASADLIITLMPLPNELGRGTPIIQVSPQLSPADIDA
                     ITSFLG"
     gene            10177..>10841
                     /locus_tag="PLL88_08480"
     CDS             10177..>10841
                     /locus_tag="PLL88_08480"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOJ01640.1"
                     /translation="MDKKELFERIKADGVKFVSYQFSDVTGKVKSVDAPLERLAEALE
                     DGVWFDGSSVEGFARIQESDMHLRVDVDSYAVLPWTPDDHKRARFFCDIYTPEGEPFD
                     GDPRGVLKKWMKKLDEKGWIYNVGPEPEFFLFLHNDHIHPVPHDVGGYFDFSSNDSAA
                     QVRAELVEALNTMGLEVEVAHHEVALGQHEIDFRFADALKTADNVLTLKYTVKAIAAQ
                     HGLV"
BASE COUNT         2659 a         2536 c         2474 g         3172 t
ORIGIN      
        1 acagaggtga tgcccatatc tgcaaagaca tccaacaatc gagccatcag catggaattg
       61 accccttttc cctgataatc cttgcgaatc gcgatcagat agagatcgcc tttattgttc
      121 tttcgtaggg cgtgcaatag gtagagaaat ccaagcggta ccaatctccc ccttgcctgc
      181 cgtagcgctt gcgagagtga gggcatggcg atgccgaaag ccaccatttt gttggtttcg
      241 tccaatacga taggtatgaa ttctggcgat atgaatccga aatactggct gatataggca
      301 ttcacctgtt cttgcgaaag gggagttacg ccgtacaaat gtcggtaaga ctcttccaac
      361 aagtcgaaga gctggtgcgc atactgcaat aattcttttt tgtttttggg ttcaagataa
      421 tgcagatggt agcgttctgc caccaccttt gccaggtttt ttatcttttc cactctgccg
      481 gcaggtactt tgatctcgaa ttccacccaa tcaatgtctt ttttatatcc ggcgctttcc
      541 aagagagcct ggtaataggg gtaattgtaa atgccaacca gcgttcccgc ttcttcaaaa
      601 ccttctacca gcattgcctc cggatccagg tcggtgaaac ccaaaggacc gtgtacggca
      661 gtttttccct gctcttttgc ccactcctcc actttggata gaaggcactg agatacctgg
      721 ggatcgtcga tgaaatcgat ccaaccgaaa cgggcataag catttcccca gcgttcctgg
      781 tagcgcgggt tgataatgcc ggcgatacga ccgacgattt tgccattttt atacgccagc
      841 cagtatctgg ctttgcagaa ggcgaaggca gggttcttat cccaacgcag tgtgtggatc
      901 tcgtcgcctc gcagtgcgcc aggaacccaa tatggatttt ttttatagag acgaaatgga
      961 aaattgacaa acttccgcag cgttctgcgg cttgtgactt ccttcaacgt aatggtcata
     1021 atggctgcat tccttcgaaa aatgataaat ggataaaaga ataattctgc tcgcttaaag
     1081 cataagactc tttatcataa caaattgcat taaaaaaagg aactagggaa ggttaatttt
     1141 tgattaatga ttgaattttt taatgaaaat cctgcgccgc ttgtgctggc gggtttcaaa
     1201 atgcttccat tgcgattgaa ctctatagtt gtcttccagt tcggggttcg attcgactga
     1261 tttgatcccc atttcatgaa aaacatccaa catggctgcc atgagcatgg cattcactcc
     1321 cctgccctgg tacgcaggtc ggatggcgat gagatataga tcggctttgt cattgttccg
     1381 taatgcacgc agcaccggca agaatccgaa tgggaacagc tttcctttgt ttttctgcat
     1441 ggctttcgaa agcgagggca tggcgatccc aaatgccacc atgcggttgt tctcatccag
     1501 cacgatcggc acgaattttg gcgggatgat ccccaggtac tgatcaatgt aggcatctac
     1561 ctgcttgcgg gtgagggggg ttaccccgta atgcaggtaa gcctcctcta tcagttcaaa
     1621 gagctgggaa gcgtattgca gaagttcttt tctgttcttt gcatccagga gatgcaggtt
     1681 gctgcgtttg gcgacgattt cagccagctt gctgatcttt tccactttct tatggggtac
     1741 tttgatctcg aattccacgt aatccgtatc cttaccgtac cctgcgcgct gcatatggtc
     1801 tttatagtag ggatagtggt acgaggctgc cagggtggct acttcgttaa acccttcaac
     1861 gagcatggct tcgggatcca ggttggtgaa gcccagcgga ccgtgaatgg cgtcctgttt
     1921 ctcggcgcgc gcccaatctt ctacggtatt caataaagca ccggatactt cgtagtcatc
     1981 gatgaaatca aaccacccca ggcgcgcgta ccgtcgcttg aaccgttcct cgaagcgcgg
     2041 gttgatgatg cccgcaatac gaccggcgat ctttcctccc ttgtatgcta accagtattt
     2101 tgctttgcag tattcaaaag ccgggttttg ctcccaatgc aaagtcttaa gaacgttgga
     2161 acggatggct ggtacccagt aggggttgcc tgcatacagt tggtaggaga aattgacgaa
     2221 ttgttttagc gttcgcaggc tggtgatctc tttgatctcg atgctcatga tgaaaaatcc
     2281 ttattgaaag ctagattttt attgcaatga tgcagcacat agtattttgt tagtgtaaca
     2341 tttattttga aagattatta aaaaccatcg gttgtgaatc catcagaatc gtccgctgcg
     2401 ctgacgctga cgagaatcaa aagacagcaa gttcttcatt gctgtctttt gatcgtttat
     2461 caaagatctc tccgagcttg tctcagcaaa ctggctcagg acgcttgagt attttgtaag
     2521 tgtttttcta attcctggta aaaggcgttc atcatggttt cgaccacccc ctgccttgcc
     2581 ccaaacatca atttttgtcc gatggcattt aagggaaagg caaagatgta gatgctggtt
     2641 cccccatttt caatttcggt gaattggatt tccagttggc ttctatcgcc caagtaagct
     2701 ccaaaaagct ttttatccat ctgggcatgc agaattcctc tttctgcatc ttgtttaatc
     2761 acctttccac ccatgtgttg gatgcaatgc acggcagatt gatatactgc ttccaacggg
     2821 aatgggagtt ctcttcttgt ttccttttca tatggcattt tgcttactcc tcacaaacga
     2881 ccgctttctc cagtagtttt tctacatccg acagtccggg catcatggtg cccggcaaac
     2941 tggcggcaac ggatcctgca gccagccccc agcggaaggc ttcgggtaaa ctatcttccc
     3001 tgctcagacg atatacgatg gcagccagca tggcgtctcc cgcaccgatg gggtttattt
     3061 catcgacttt tggaggaatt ccgacccagc gctggttgcc attgctgcaa attgcttttt
     3121 ccttgccggc agataggatg atgtttttca ctcccatggc atggattctg gatagggcct
     3181 cctcaagcgc aagggcgtcc tgcacgttca ggtctgtgat gtgcgtgagt tcttctacat
     3241 tgggcttgat gaggaacggt tttgccatgc aaccgtagtt caaggcttca ccgctcgaat
     3301 caaggatggc gcgtgccttg cctcggttga tgcgttggat catttgtgca tagatatcat
     3361 gcggaacacc ccgcggtagg ctacccgaca tgatccacca atcttctgga tggatcaatt
     3421 cttcgacctt tcgcagcaac tgctctatct cttcaggtaa aatttcagga cccgcctcgt
     3481 tgatcttgac ataactcctg tctttatcgc tgacggcgct gatattggtg cgggtttctc
     3541 cgctgatttc tacaaaatcg gttttgatgc cattgctttt caaaccttca cgcaggatac
     3601 tgcctgtgtg ccccccgata aaaccgagtg ctgcacaatc agcatcgaat gttgagagca
     3661 ttcttgcaat gttgaatcct tttcctccga agtcaatttt taccgatgat gctcgcagga
     3721 tcttgttggg tagcaattct ggaacgcgat attccttgtc cagtgccggg tttaatgtca
     3781 ccgtatagat cattggcaga tttctccgga gggaaggggt tgacgcgaaa gcagtaaccc
     3841 aagggttgcc ccgccgagcg cagattttgc aatcgcgagc ccctctcctc ttttcactgg
     3901 gcgctccccc tccagagttc ctcgatcagc gctttttccg cttcgattgt ccataaatag
     3961 ttctctccaa gcttttcagc aatttgagga tagctatttt ctaggtcttg taagccggtt
     4021 agcggaagtt cgtttatctg gggaaaaatg atgattttgc cagcgaacgt tccgtttatc
     4081 aacgcttcca atccctcgtg ggctgcttgc attccaccaa tcgctgcaac tgaccggttt
     4141 gggtccaact ctccccgcaa cgtcttttgc agcacaactt tctgatcgtg aagctttgac
     4201 ccggaagttc ccgttaattg caaattgccc agaaagaccg aactgaggtt catgtttatc
     4261 aaagtgccgg tgggtacacc ggcaaagagt atcaacatgc catcaggctt taagattttt
     4321 gcagtatctt ccatcagttt tgctacggga gtgcagatga cggcatcgtc aacgcatctg
     4381 tctcctgaaa tctcgaaaat gaaatcttgt aatgattgtt ctgtttcgtt ggagttgaat
     4441 attttaaaaa ctttgctttt tttcatagcc aggggttcaa tgatcttctt taaaacctca
     4501 gccctttctt tattcaattc tgttgcgatc actatagccg gaccgtctgc tttttcaatg
     4561 gcacgttgta cgtgcatttg ccccatcgga ccacccgctc ctataaatag ggcagttcca
     4621 tttttcctta attcacagcg gttccgctcc tctccatatg cggcggaaat atcagaactt
     4681 cttgttccta cataggctgt gtagtggtag tggatgcgcc ctgcgtctat gagggaatct
     4741 ccatccagtt ctttgttccc tacaatgttg agaacccctc tgaaagcgat gaattttgcg
     4801 atttcctcta cgaattttcc tgaggtgggg gatagaagga taatatcgtc gaatccttcg
     4861 ccgttcgttg agcgggcaga aaaatcggcg atttcttcga gataaatttc tttcgtttca
     4921 atgatgtgtg tttccgtagc gacattcttt ttgatgattt ccttgaactt gtctggaatg
     4981 ttggtgagaa agatcgtttt tggcgctccc agtccttcgc tgaaagcata ttgcgttgta
     5041 tcatccttgt ttccgactat ccacatggtc ccgccggttt tagggttcag tcttctgcgt
     5101 tgggtatacg cagcatccac gcatgcccag ggttctgtca gggcagcttc agcgtacccc
     5161 accctgttgt cgaccggaat cacataggca ccatcatctg cgtcaagcac ttctgctccc
     5221 attaaatggt actgggttaa accgcctggt atggtgtagc cgtaagcggt gttatgttgg
     5281 tttacataga tatccggttg aatggctaat cgttgcccgg gttggtattc atctgcgaga
     5341 tttttcccaa ccttgatgac ggttaatgcc acttcatgac caactctggt cggctctgcg
     5401 gatagatcgc gaccggaaag ttttgggtgc tcaccgccca gtttgatcag ttttacatca
     5461 gaaaagcaga gccccaccgc atccaccctt accaggagct gattttcatc agggggttgg
     5521 attttgaaag attctggttt cccctctctg ccaatattct caacccctgc accatacagg
     5581 ttccaggtca aagtagactc tggtattttc aggtcgtttt gatattccgc aaatttggaa
     5641 ttcttcatta acgtacttcc tttctactgc actctttcga gcagagaatt tgtcaagttg
     5701 gtgggatccg tttaagttga ctgtttccct ctttttgttt atacgatctc attcttccaa
     5761 caaaccttta tcgatgcatt ctttcagggt tgcaaatacg gtttcttcgt cttcgccgtt
     5821 gatctcaacc agaatgttcg ctccactttc ggctcctaaa gtcagcagag atatcagact
     5881 ttttccgttt gctttttttc cattgtagaa tacggaaatt tctgctgcat aatctttgac
     5941 cagattcacg aaatttttag caggacgtgc atgtaagccc gtaggattac ttaaagagat
     6001 gttcagtttc ttcatgaaag acctgttgac gccgaattcg atctctttat tcggtcgatg
     6061 atttattttc tttgttgaga tgatccaaaa ttacgttgac attgcgtgtg gttctcatct
     6121 tttgtgctat ttcttcatcg tcaatggctt ctgccagatt ggctagaata gtaacgtgtt
     6181 cgttgcgcaa ggctgcaatt ccaatgacca gatatacctg ctccccttca tcccattcaa
     6241 ctccttccgg gatctgcaaa acggaaatgc agctattgaa gatatcgttg cggttgtcga
     6301 attgtccgtg agggatggcg actccgttgc ctaaataggt cgacataatc tcttccctcg
     6361 ccagcatccc gtctacataa ttggggctga cacaacctgc ctctaccagg agctttccag
     6421 attggcagat ggcatcctgt ttgtcctttg cctttacgtt gagccgtata caatccttag
     6481 aaaggatgct catgatttac tccacattgc tggtaatatc ttcttttcga acgaaggcgg
     6541 agattatttt gtcgaaaatt ggatcgttta aaaactgatt gaaagccaaa ataaccgcgt
     6601 taggagcttt tgcacaggca acttttgcaa gacctctatg gacaattacc acttgagcat
     6661 cgccaggaat atcacgtgtg gctaggtgaa atacttcaac accttcaata tttgctttct
     6721 tcattttctt tttcagggag ttaacactca tcaaactgga acccatgcct gcttcacagg
     6781 caaatagtat tttcttaatt tccgaagtgc tgatcgattt agccattttt aaacttcctt
     6841 tttcaaaatt gagaaaaggt ttcaatgact tccatagctt gaaacctttt ctcgtggata
     6901 ttatttattt ggaaagtccg ggaactttgg ttgtttcttc tgcttcttct tgcttttctt
     6961 caggcaatgg atttgccttc aaaataagag tcccgactaa gaaagaagcg accgcaccga
     7021 tcaaaacgcc aaccaatact gcaaaatgtt gtcctttggg gatcactgcc aggtatgcga
     7081 aaatggaacc cggtgatggg gttgcaacaa gaccggcatt catgatggtg aaccacagat
     7141 tcgctgccag tccgcctgca atgacggata ggatcatgat tggatgggca agaacgtagg
     7201 ggaaataaat ctcgtgaatt ccgccaagaa agtgtataat cattgcgccc ggtgctgact
     7261 cttttaacaa acctttacca gcaacgtaat aggcaagtaa aagaccaagg ccgggccctg
     7321 gatttgtttc caataagaag tgaatggctc ttcccgattg ttctactgca gcaacaccca
     7381 agggggctaa aacgccgtga ttcaacgcgt tgttcatgaa caacaccttg gcaggctcaa
     7441 tgatgagggc gaccaatggc agaatctttg cattgatcat cgaatccacc acgccaccag
     7501 cccagttgct gacgctttcg accacgggac cgatgagcac attgcccagc aagacgagca
     7561 gaagccccaa gatgccagca gtgaagttgt tgatgagcat ttcgaagccg acaggaattt
     7621 tatcttcgat cacatcatca attttcttga ttaaccagcc gcctaaagga cccatgatca
     7681 tggcgcccag gaacattgga acgcccgaac ccactacaac gcccatggtt gcgatagcgc
     7741 cgaccacgcc acctcttgtt ccatgcacca ttttcccgcc ggtatagcca atgagaattg
     7801 gcagcaaata agtgagaatg ggactgacat ttgctgcaaa cttttcattc ggtatccaac
     7861 ctgttgcaat gaacagcgct gtgattaaac cccaggcaat gaatgcgcca atgttaggga
     7921 taatcatccc agccatggaa cctcctaacc tttgtatctt ttctttcatg tttatctctc
     7981 ctttttttta tttttgaagt ctcttttttt atcgtcagcg ctgtcggcac gctgacgaat
     8041 aaaacgaatt catcctaaga atgaagtgat tgcatcaata tctgccggtg agagctgagg
     8101 agatacctga atgattggcg ttcctctgcc tagctcattt gggagtggca ttaaagtgat
     8161 gatcagatct gcactggcca ctctttcaga atttaattca cgaaaggaaa ccacgcttaa
     8221 tttccctaaa tgcggaaatc tcgttttgag acgcgctgta agcagttgcg cggttgccat
     8281 tccacttgga caaaccagca gaacgttgct gaactgatac gatcgcaatc gtatatatgc
     8341 tgccctgata aaagcgacga gcatatattc gtcttctctg tccagggtga tggaagtatg
     8401 tgtgtggatg atgctcacca actggtctgc caggaataag tcgtcgtcaa acttgaaagc
     8461 gaatgacaga tcaactgttg ccttgggaaa ccacaaattg taggagtgct ggctgaagat
     8521 cgggattaga tagtttatga ttccgtctcg taaagtgggg tcatccttca acccgtcgat
     8581 tttatacaag gaattgatgg cttccagtaa ttcttcaaat agatctttat atccccctgt
     8641 tttatctaca tcaccgatcc aattttcgag caatggtgaa gacagaatat acatgaccac
     8701 ataggcaata tcgccgggtt gccaatctga aatattacct tgaccgagat ttttgatcat
     8761 cttctcggca gctttccact cctgcatttt taggaaatcc gtgagctcct gatcggtgac
     8821 ctggatgtgg ttgcccgttt tgattcttgc cgataaaact gaaaaaacca aagctaagta
     8881 caatactgct tcatccgtga atcgacctcc cagagaatct tcaataaaaa tgaccttgtt
     8941 gaagattctc ttcatattga gtaagcatag gaaatcgttg atctctttga agaggggagt
     9001 attctgaatg tcttcggcta atgtgaaagt taacccttct tggaacgata ctccaaagac
     9061 tgagggatag aacggacatt tgccccataa tagagccaat aacgcttgtt gtttattttt
     9121 ctcggtgctg gtgatgtgaa ttccatagtt ttgttttctt gttaaagata tgccccgctc
     9181 atccagccac ttttcaactt tatccaggtc ttcaacaatc gttgagcgag agaccttcag
     9241 gtaaaagcta agttgggtga gaagaactgg ttctgctttg atcagcagta gaaaacaaat
     9301 taattgttgg cgttgttcgg gtgcaagtac aagttgtaaa ccctcgtccc gttctaactc
     9361 tgttgccaga gaaactcgct gttcaggtag acaaaccagt ttaatccccc ttcccggagt
     9421 cgtttgcaag gttacccttt tggtggttag ccaacctttc acccctttca tgctgtagtg
     9481 cacctgacga ggtgtcagtt ggatttccat cgccagatca tacgtccgga tgggattgtt
     9541 cgtttttagt agaactctta aaatatcacg ttggcgagta gttaaagaaa ccataaaatt
     9601 tgttcttcac aattgtcgca ttagtctacg tttgttatta tatagaacag aaaaacaaaa
     9661 gaaaacaggc aatacatgtt gcttttcgta gcatggcaat gacaaaaaca atataattgg
     9721 cgtggaatat gcgaacggat ccctgtgtca gaaaattttt tcccatgctc tatgacaaat
     9781 tactgaaaat ccttgacaaa cttcctattc tctgataaaa atgcgtctaa ttaagaacct
     9841 tacattgcaa aaacggagaa atagcggctg ggtctcaaac ggtgagatca cgaggtgaag
     9901 gttccatccc gcgagggatg gttaacccgg gaaaccgggg aagatcggat tgatcagcgg
     9961 atgccttcgg agttttgcca cagactcctt tctcccttcc gaaagaagcg caaggtgaaa
    10021 ccccgccagt gatggtggta agaagccgac cgcggtggtc aggatgtcaa tcctgtgtta
    10081 ataaatggaa gggcagcttg catatttttt tgtgataaac aggcatgccc ggcatgcctg
    10141 tttgtttttt aaatgtcaat gccaaaggag agaaacatgg acaaaaaaga gttatttgaa
    10201 cggatcaaag cggatggggt gaaattcgtc agctatcaat tttcggacgt gaccggaaaa
    10261 gtgaaaagcg tggatgcccc gctggaaagg cttgctgaag ccctggaaga tggcgtttgg
    10321 ttcgatggct catctgtaga aggttttgcg cgcattcaag aaagcgatat gcatttgcgt
    10381 gtggatgttg attcttatgc cgtgctgcct tggactccgg atgatcacaa acgtgcgcgc
    10441 tttttctgcg atatctacac ccctgaaggc gagccttttg atggtgatcc gcgcggagtg
    10501 ctcaagaaat ggatgaaaaa attggatgaa aagggctgga tctataatgt aggaccagag
    10561 ccagaattct tcctttttct ccacaatgac cacattcacc ctgttccgca cgatgtaggt
    10621 ggttattttg atttctcgtc aaacgactct gcagcccagg tgcgcgctga actggtagaa
    10681 gcgctgaaca ctatggggct ggaggtggag gtggctcacc atgaggtggc gctcggacag
    10741 catgaaattg actttcgctt tgccgatgcg ctgaaaacag ccgataacgt gctgaccttg
    10801 aaatatacgg tgaaagccat cgctgcccaa cacgggttgg t
//
LOCUS       DAOSDS010000073        10711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2355_length_10711_cov_4.415259, whole genome shotgun sequence.
ACCESSION   DAOSDS010000073 DAOSDS010000000
VERSION     DAOSDS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10711
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2355_length_10711_cov_4.415259"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /gene="murD"
                     /locus_tag="PLL88_08485"
     CDS             <1..1323
                     /gene="murD"
                     /locus_tag="PLL88_08485"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOJ01641.1"
                     /translation="LARFLSKLQAHVTLNDNQPDENFSDLTQELLGLGIQTCFGSHPL
                     TLLEGCDLLCISGGVPLDLPIVQEAIRRRIPLSNDSQILLERLKTKTIGITGSAGKTT
                     TTTMVGEIAKKACRNAQRAWIGGNIGTPLITYLDEIHSNDIVVLELSSFQLELMTLSP
                     SIAAITNITPNHLDRHGTMQAYTAAKARILAHQGSHDTAILNRENAEAWNLREMVHGR
                     LITFGMKEPQGDLPGTFLRDHSLWIKDGQRQQELCPVHAVQLRGEHNTLNALAASAIA
                     YAAGFPPDAIESGIHEVRGISHRLEFIRRWHGADWYNDSIATTPERTLAAIRSFHEPL
                     VLLLGGRDKHLPWEELAAEVHKRADHIILFGEAADLIYTALDAHEQHQFPYTLDKATS
                     LSDAIQTAAGIVQEGDVVLLSPGGTSYDAFKDFEERGSLFKKLVEELQ"
     gene            1320..2564
                     /locus_tag="PLL88_08490"
     CDS             1320..2564
                     /locus_tag="PLL88_08490"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HOJ01642.1"
                     /translation="MNQKTTIHKKKERSLHLGIDVPFLVCILSLLAIGLLMVYSAGWE
                     YSMTTLGEEANYLFKRQLRFVILGCVGATLIYSIDYHRFKKLLFPMMATIIILLILVS
                     FVYGETRLGAKRSLFQGSIQPSEFAKLTVVIYLAFWLSVKQEVLDSVKFGLLPLAGIL
                     GVIAGLIMAQPDFSAVLTVVLMGGIMFFIGGGNIRQIIIVMAIMLVLGSILIFLYPTA
                     HTRVSDFVAGLQDPQNASYQTQRSIEAIVKGGFWGAGIGHATTKFTGLPVAPTDSIYA
                     VVVEELGLFGASVVALLFLFLLWRGLYIANQAPDLLGKLLAAGVSLWIFTEAAINICV
                     MVNILPIAGNALPFISAGGSSMVTVLAGVGFILNVSRATANQKNKEEGRPIDEVVDLR
                     GNDGWRSVSRSRRSPNAHQRSR"
     gene            2476..3579
                     /gene="murG"
                     /locus_tag="PLL88_08495"
     CDS             2476..3579
                     /gene="murG"
                     /locus_tag="PLL88_08495"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOJ01643.1"
                     /translation="MKLLICAGMTGGGVYPALAVLQTLTKEVDEILWVGSEGGMENEI
                     ITRLGIPFKAIPAAGLHGVGLQKLPGNLSKLARGYQEARTIIHTFRPDVFFSTGGYLS
                     VPVAMAARHIPSVVFIPDIEPALALKVLIRYADRVLTSTAETEQFVPQKGKITLCGYP
                     LRREITRWKKSHARKELALNNELPVLLVFGGSKGAQSINRALYPLLSEFLEVAQVVHI
                     SGLDNYEETREKSSALSDTLKKRYHLFPYLHENMGAALAAADLVVCRAGASTLGELPF
                     FNLPAILVPYPHAWRYQKTNADHLAHAGAAIILQDEELSRSLKSTVLDLLCDPQRLQN
                     MQQAMRALSTPDAAEKIGKIIVEVGTKKNREEQ"
     gene            3581..4120
                     /locus_tag="PLL88_08500"
     CDS             3581..4120
                     /locus_tag="PLL88_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01644.1"
                     /translation="MISLGVLFWIFIMMFALIGAIRGWTKEVLVTFSAVMGIFAITIL
                     ETYVPFVKTYLASNPITSKVWLRIVIFIVLVFFGYQTPNIPRFSGDERLARGRLQDIL
                     LGLIIGGVNGYLIYGSLWYFLADGGYPFEILIPPIAGTPAGEAALALVPYLPPVWLTT
                     PTIYYAVAGSFIFVLVMFI"
     gene            4117..5493
                     /gene="murC"
                     /locus_tag="PLL88_08505"
     CDS             4117..5493
                     /gene="murC"
                     /locus_tag="PLL88_08505"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOJ01645.1"
                     /translation="MKKHYHLIGIGGTGLSAIARVLLEQGHTVSGSDALISPLVEELC
                     NQGATVYTGHKAEQIAGADMVIRSSAIRDDNVEVKAAQKAGIPVLKRVDFLHELTAQH
                     KLVAVAGTHGKTTTTAMAAWVFHQLGLDPSFIIGGTSKDLNSNAHAGKGEYFIIEADE
                     YDSMFLGLAPDYLILTMVEYDHPDCYPTREAYALAFQNLIGKMEKNGVLLANADHPQT
                     LNIGKRAEQSHKVLYYGRTPKTDYQIKDIHHKEQQGVGFRLQLPNGQIIAQELSIPGD
                     HNAANATAVLALVDEMGLSINTASQALFEFSGTGRRFDILGTFQGITLIDDYGHHPTE
                     IRSTIQAARSKYPHQRIFTIWQPHTFSRTQELFEDYLDAFRGSDVVIVTEIYASREKP
                     QNYSAALVASQIRNAEVHFIATLNDVTKFLLHEVEDGDVVLTLSAGDAYQINQQLVTS
                     LKEGRIHA"
     gene            5486..6457
                     /gene="murB"
                     /locus_tag="PLL88_08510"
     CDS             5486..6457
                     /gene="murB"
                     /locus_tag="PLL88_08510"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOJ01646.1"
                     /translation="MPDTDLKRLQERFGNKLQENVQMKHYTTIQTGGLADALLVAQDA
                     QELEAYTQGVWELGLPLLVLGSGSNILVSDRGIREVVVINHAHNVTINSHPGRILVNA
                     ESGALMSKVARQAINRHLSGLEWAATLPGSVGGAVYGNAGCFGKETADSFVQAEILHR
                     DSGKAIYRKEAMAFEYRSSVLKRNREACVILTASFAAQPGDHDRIMAEVEEFKNRRQR
                     TQPPGPSMGSIFRNPQCDKAGKLVESVGLKGKKYGGAEISAQHANFIVNSSGGSAQDV
                     WNLIQIIENAVQHKFGFYLYPEIQLIGDWDAEILQEFERYQTTQENA"
     gene            6454..7437
                     /locus_tag="PLL88_08515"
     CDS             6454..7437
                     /locus_tag="PLL88_08515"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOJ01647.1"
                     /translation="MNKIRIGILFGGRSGEHEVSLMSSKSVLSVLDPQKYEITEIGIT
                     HAGEWWSGENTRQKFQQNSFDGLYRVLLLPEPGKSVLYKHLEEKLEALTELDVIFPIL
                     HGTFGEDGTLQGFFEMADIAYVGAGVLGSSVGMDKALFKDVMRSNDIPVADWIVISRK
                     EAEKNIDALIRTAEELGPYPFFIKPANLGSSVGISKCKNRSDLLEGIMQACRFDRRIL
                     IERGVENAREIEVSVLGNESPRVSLPGEIKPSDEFYSYNAKYIDDRSELIIPAPLSKE
                     IITEIQEMAIRAYQAIDCAGMARVDFLLNRNTNQIYLNEVNTIPGFTKISM"
     gene            7556..8575
                     /locus_tag="PLL88_08520"
     CDS             7556..8575
                     /locus_tag="PLL88_08520"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOJ01648.1"
                     /translation="MKKKDHAEPLKRSDILRQKRQEEQNEWLHTVNSRAEHMASRPAF
                     EALEENQHEYRPLNDSQNPLAHIAIDNKEYATPRIRLSWRILSGAMAVISLVLLISAW
                     RSPDYHVSDVDIQGIHRISKEEIAAALNINQQRVFALSPTVIEQTITEDFPELYDVKA
                     SITFPAKVEINVKERQPYLTWNYQGKSIWIDTDGYLLPQRGDAEITLTIESTEKPPFY
                     IPEDRVILKGEKRLHKTVVAKGENDTLALFNVYQKIEPITYRAIKDLNQLLPEQDLIL
                     YDARRGLGWNDPRGFKVYIGSTLQDITAKMNMIEEIVKTLIKAAVAPTLISVEQINAP
                     YYRLD"
     gene            8581..9813
                     /gene="ftsA"
                     /locus_tag="PLL88_08525"
     CDS             8581..9813
                     /gene="ftsA"
                     /locus_tag="PLL88_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOJ01649.1"
                     /translation="MEEEIIVGIDIGTTKVCTLIARVEDDNAFCILGVGIERSDGMRK
                     GMIIDLQKTTNAIAKSIEKAQRSSGYEITSALVSIAGSHVSSINSRGAVGITNGVVSQ
                     IDINRALEAAQVVAIPHDREVLHVIQRGFTIDGQDGINEPLGMHGYRLEVETHIITAS
                     AASVGNIRKCVEAAGVKIKAFVLNPLASAEVVLSETERQLGVMVCDIGGGTTDLAVYI
                     NNNVWYTTVISVGGDLVTSDIAYGLRLPLPDAEKVKIAYGRAKESLTSGNETFSIRSF
                     GEEEATEFRYADLAYIIEARTEEIFQFVLQELKRSGYDGLLPAGVVLTGGSSLLPGIR
                     DLASDVLGLPVRVAQPDDLTGLVDQLYSPAYSTSVGLLKWAGILARAVKRSTRTRGLP
                     PIGALDRVKDFIKRFLPQ"
     gene            9855..>10711
                     /gene="ftsZ"
                     /locus_tag="PLL88_08530"
     CDS             9855..>10711
                     /gene="ftsZ"
                     /locus_tag="PLL88_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOJ01650.1"
                     /translation="METNSYQQGESFARIKVIGVGGGGCNAVNRMISEGIRGIEFVSI
                     NTDGQALLFSKAETRVRIGDKVTRGRGAGGVPEVGRKAAEESSEELYQVLKGSDMVFV
                     TAGMGGGTGTGAAPIIAQIAKEVGALTIGVVTKPFTFEGVQRIQAAEVGITKLKEQAD
                     TLIVIPNDRLLQIVDKRASLEEAFSAADDVLRQGIQGISELITVPGRINLDFADVRTI
                     MSEGGAALMAVGRGSGENRAREAAERAISSNLLDITIDGAHGILFNVTGGPDLTLFEV
                     NEAAAIIKET"
BASE COUNT         2810 a         2607 c         2687 g         2607 t
ORIGIN      
        1 cttgcacgct tcctctcaaa attgcaagcg cacgttactc ttaacgataa tcagcccgat
       61 gagaatttca gcgacctgac ccaggaactc ttgggattgg gcatccaaac ttgttttggg
      121 agccatcctt taaccctgct agaaggatgc gacctgttgt gtatctcagg gggcgtacct
      181 cttgatctcc cgattgttca agaagcgatt cgtcgccgta ttcccctttc gaatgattcg
      241 cagatcctct tggaacgatt gaaaacgaaa accatcggca tcaccggctc ggctggcaaa
      301 accaccacta ccacgatggt aggagaaatt gcgaaaaaag cctgcagaaa tgcgcaaaga
      361 gcctggatcg gcggcaacat cggtacccca ctcatcactt atctcgatga aattcacagc
      421 aacgacattg tggtcttaga gctatccagt ttccaactgg agctcatgac gctctcaccg
      481 tccatcgcag ccatcaccaa catcaccccc aatcatctag accggcatgg caccatgcag
      541 gcatacacgg ctgccaaagc ccgtatcctc gcccatcaag gctcacacga tacagccatt
      601 ttgaacaggg aaaatgccga agcctggaat ttgcgagaaa tggtacatgg aagattgatc
      661 acatttggta tgaaagaacc ccagggagat ctccccggca cttttctgcg cgatcattct
      721 ttgtggatca aagatgggca aagacaacag gagctttgcc ctgtccatgc tgtccagcta
      781 cgcggcgagc ataacacgct taacgcattg gctgccagtg ccatcgcata tgcagcgggt
      841 tttccacccg atgctataga atctggcatc catgaggtac gtggcatcag ccacaggtta
      901 gaattcatcc gccgctggca tggggcagac tggtataacg attccatcgc taccacaccc
      961 gaaaggacac tggctgcgat ccgctctttc catgaaccat tggtcttgtt gttgggcggc
     1021 agggataagc accttccatg ggaggagctg gcagcagaag ttcataaacg cgccgaccac
     1081 attatcctgt tcggcgaagc agccgatctc atctacactg ccctggatgc tcacgaacag
     1141 caccagttcc cttacacact ggataaagcg acctctcttt ccgatgccat tcaaacagca
     1201 gcgggaattg tgcaggaagg cgacgtggta ctgctttctc cgggtggaac cagttacgat
     1261 gcattcaaag acttcgaaga acgcggtagc ttgttcaaaa aactggtgga ggaattgcaa
     1321 tgaatcaaaa gacaaccatc cacaagaaaa aagagcgctc cctccatctt ggcattgatg
     1381 tgccattttt ggtttgtatt ctgtcgttat tggctatcgg tttattaatg gtctattcgg
     1441 ctggctggga atactcgatg accaccctgg gcgaagaagc caattatcta ttcaaacgcc
     1501 aattacggtt tgtcatcctt ggctgtgtcg gtgccaccct gatctattcc atagactatc
     1561 accgattcaa aaaactgctc tttccaatga tggcaaccat catcatcctt ctaattctgg
     1621 tttcgtttgt ttatggtgaa actcgcctgg gtgccaaacg gtcgctgttc cagggttcga
     1681 tccagccatc agaatttgcc aagcttactg ttgtaatcta tctggctttt tggttgtctg
     1741 tcaagcaaga agttctcgat tcggttaaat tcggactgct cccgttggca ggaatccttg
     1801 gggtgattgc aggtttgatc atggcacaac ctgatttttc agcagtgctt accgtggtgc
     1861 tgatgggagg aatcatgttt ttcatcggcg gagggaacat ccgtcagatc atcatcgtga
     1921 tggctattat gctggtgctt ggatcgatct tgatcttcct ttaccctacc gcgcatacac
     1981 gcgtaagtga ttttgtagct ggtctgcaag atccgcaaaa tgcatcgtac caaacccagc
     2041 gttccatcga agcgatcgtg aaaggcggtt tctggggtgc cgggatcggg catgcaacaa
     2101 cgaaattcac cggcttaccg gtagctccta ccgattccat ttacgctgtc gttgttgagg
     2161 aattgggact ttttggcgcg tctgtggtcg ctttattgtt cttattcctg ctatggagag
     2221 gattgtacat agccaatcaa gcacctgatc tattgggcaa gttattggca gccggcgttt
     2281 cactctggat tttcaccgag gcagccatca atatttgcgt gatggtcaac atcttaccaa
     2341 tcgctggaaa tgcattacct tttatcagtg cggggggttc cagcatggta acggttttgg
     2401 ctggggttgg cttcatcttg aatgtttcca gggctacagc caatcagaaa aataaagaag
     2461 aagggagacc catcgatgaa gttgttgatc tgcgcgggaa tgacgggtgg cggagtgtat
     2521 cccgctctcg ccgttctcca aacgctcacc aaagaagtag atgagatact ttgggtcgga
     2581 agcgaaggcg gtatggagaa tgaaataatc acccgcctgg gcatcccctt taaagctatc
     2641 ccggctgccg gattgcatgg cgtcggtctg caaaagctcc cagggaacct gagcaaacta
     2701 gctcgtggtt atcaagaggc gcgaacaatt attcatacat tcaggccaga tgttttcttc
     2761 tcaaccggcg gatatctttc tgtaccggtg gcgatggcag cccgtcatat tccttctgtg
     2821 gttttcatcc ccgatatcga accggcattg gcattgaagg tcttgattcg atacgccgac
     2881 agggttctta cgagcactgc ggaaaccgaa caattcgtgc cccaaaaagg gaagatcacg
     2941 ctgtgtggat atcctctgcg aagggagatc actcgttgga agaaatccca cgctcgtaaa
     3001 gaattggcac ttaataatga gttgcccgtg ttgctggtgt ttggaggcag taaaggcgcc
     3061 caatccatca atcgcgctct ctatcctttg ctttctgaat ttctggaggt agcgcaggta
     3121 gtgcatatca gcggattgga taattatgaa gaaacccgag aaaaatcgag tgcgttatct
     3181 gacactctga aaaagcgcta tcatctattc ccctacctgc atgaaaacat gggagctgcc
     3241 ctggctgctg ccgatctcgt ggtatgccgg gctggtgctt ctacccttgg cgaactgccg
     3301 tttttcaacc ttccggcgat cctggtgccc tacccgcatg cctggcgcta tcaaaaaacc
     3361 aatgcagatc atctggcgca tgccggcgct gcgatcatcc tgcaagatga agagctatcc
     3421 agatcgttga aatccaccgt ccttgatctt ttgtgtgatc cccagcgttt gcaaaacatg
     3481 cagcaagcca tgcgcgcgct ctccacccct gatgcggcag aaaagatcgg taagatcatc
     3541 gttgaagtag ggactaaaaa aaacagagag gagcagtagc atgatcagcc tgggtgttct
     3601 tttctggatt ttcatcatga tgtttgctct gatcggcgca atcagaggtt ggaccaagga
     3661 ggttttggtc acttttagtg ctgtcatggg tatctttgcc attaccattc tcgaaaccta
     3721 cgtgcctttc gttaaaactt atctggcttc caatcccatc acttcaaagg tctggctgcg
     3781 tattgttatc tttattgttc tggtcttttt tgggtatcaa accccgaata tcccgcgctt
     3841 ttctggagat gaacgtttgg cgcgcgggcg gctgcaggat attcttttgg gattgatcat
     3901 cggcggtgtg aacggatacc tcatttacgg ttcactctgg tatttcctgg cagacggggg
     3961 ataccccttt gaaatcctca ttccacccat tgccggaaca ccagccggag aagcagcgct
     4021 ggcgcttgtt ccctatcttc cgccggtctg gttaactact cctaccattt actatgcagt
     4081 ggcaggatca ttcatttttg ttttggtcat gttcatatga agaagcacta tcaccttatc
     4141 ggaatcggcg gcacagggct ctcagctatt gcgcgtgttc ttttagaaca aggacacaca
     4201 gtaagtggtt cagatgcttt gatttctccg cttgtagaag aactgtgcaa tcagggtgcc
     4261 acagtctaca cagggcacaa agcggaacaa atcgctggtg ccgatatggt gatccgctct
     4321 tccgccatcc gcgatgacaa tgtggaagta aaagcagcac agaaagccgg cattccagtg
     4381 ctcaaacgcg ttgattttct ccacgaactt acagcgcagc acaaacttgt cgcggtagcc
     4441 ggaacacacg gaaaaaccac caccacagcc atggctgcct gggtttttca tcagctaggt
     4501 cttgatcctt ccttcattat tgggggaacc tccaaagacc tcaacagcaa tgcccatgca
     4561 gggaaagggg aatatttcat aatcgaagcc gatgagtatg attcaatgtt ccttggtcta
     4621 gctcccgatt accttattct caccatggtg gaatatgacc acccagattg ttaccctact
     4681 cgtgaagcgt atgctctggc ttttcaaaat ctgataggga aaatggaaaa aaatggcgtt
     4741 cttttggcaa acgcagacca cccgcagacg ttgaacatcg gaaagcgggc agagcaatca
     4801 cacaaggtgc tttattacgg acgcaccccg aaaactgatt atcagattaa ggatattcat
     4861 cacaaagaac agcaaggcgt aggattccga ctgcagcttc ccaacggaca gataatagcg
     4921 caagagttat ccattccagg cgaccataat gccgcgaacg ccaccgccgt gctggcgctg
     4981 gttgatgaaa tgggactttc catcaacact gcttcgcagg ctctattcga attctctggc
     5041 actggaagac gttttgacat tcttggcact tttcaaggga tcacgctgat tgatgattat
     5101 ggacatcatc ccactgagat ccgttccacc atccaggcag cccgcagcaa ataccctcac
     5161 caacgtattt ttacgatctg gcaaccgcat accttcagcc ggacgcaaga actctttgaa
     5221 gactacctgg acgcgttccg aggcagtgat gtggtcatcg tcacagaaat ctacgcgtcc
     5281 cgtgaaaaac cgcaaaatta ttccgccgcc ctggttgcat cgcagatacg gaatgccgag
     5341 gtgcatttca tcgccacttt gaacgatgtt accaagtttt tattgcatga agtggaagac
     5401 ggtgatgtgg ttttgaccct ttcagccggg gatgcctatc agatcaatca acaattggtc
     5461 acctccttaa aagaagggag gatccatgcc tgatactgat ctaaaacgcc tgcaagaaag
     5521 atttgggaat aagttgcagg agaacgtcca gatgaaacac tacaccacca tacaaaccgg
     5581 tggcttggct gatgcgcttt tggtcgccca agacgcacaa gaactggaag cctatacgca
     5641 gggtgtctgg gaattgggtt tgccattgct agtattgggc agcggatcga acatacttgt
     5701 cagcgataga ggcatccgcg aagtggtcgt catcaaccac gctcacaatg tgaccatcaa
     5761 cagccacccc ggaagaatcc tggtgaacgc cgaatcaggg gcattgatgt ccaaagtagc
     5821 ccggcaggct atcaatcgtc atctttctgg attggaatgg gcagcaacat tacccggaag
     5881 tgtgggaggg gcggtatatg gcaacgccgg ttgcttcggt aaagagacag ccgattcttt
     5941 cgttcaggca gaaatcttgc accgagattc aggaaaagca atatacagaa aagaagctat
     6001 ggcatttgaa tatcgttcca gcgtcttgaa acggaatcgt gaagcatgcg tgattctgac
     6061 cgccagcttt gctgcccaac cgggagacca tgaccgaatc atggcggaag tggaagaatt
     6121 taaaaatcgc cgccaacgga cacaacctcc cggccccagc atgggatcca ttttcaggaa
     6181 tccacaatgt gacaaagctg gaaaattagt tgaaagtgtg ggactgaaag ggaaaaaata
     6241 cggcggggct gagatcagcg ctcaacatgc caatttcatc gtcaacagtt ccggaggcag
     6301 tgcacaggac gtttggaatt tgatacaaat catcgagaac gctgttcagc ataaatttgg
     6361 gttctatttg taccccgaga tacagctaat cggtgactgg gatgctgaga ttttgcagga
     6421 atttgaaaga taccaaacca cacaggagaa tgcatgaata agatccgcat tggtatactc
     6481 tttggggggc gctcgggaga acacgaggtt tcactgatgt cttcaaaatc tgtgctttcc
     6541 gttctggacc cccagaagta tgagatcacc gagatcggca tcacgcacgc gggggaatgg
     6601 tggagcggtg agaacacccg gcaaaaattc cagcaaaatt cctttgacgg tctgtaccgt
     6661 gtcttgcttc ttcctgaacc tggcaagagc gtgttgtaca aacacttaga ggagaagttg
     6721 gaagctttga ccgagttgga tgttatcttc ccgatattgc acggcacttt tggagaggat
     6781 ggtacgctac aagggttctt cgaaatggca gatattgcct atgttggcgc tggcgtactt
     6841 ggctcatcag ttggaatgga caaggctctt ttcaaagatg taatgcgttc taacgatatt
     6901 cctgttgcag attggattgt gatcagccga aaagaagcag aaaaaaatat cgatgctctt
     6961 atcagaactg ctgaagaact tggaccctat ccgtttttca tcaaacctgc caacctgggt
     7021 tcttcagtgg ggatcagcaa gtgcaagaac cgttctgacc tgttagaggg gatcatgcag
     7081 gcttgccgtt ttgaccgtcg cattttgatc gagcggggcg ttgaaaatgc ccgagaaatc
     7141 gaggtcagcg tattgggaaa tgaatccccc cgcgtttcat tacccggaga gatcaaacca
     7201 tccgacgaat tctacagcta caacgcgaaa tacatcgatg acaggtctga gttgatcatc
     7261 cctgccccat tatccaagga gatcatcacg gaaattcaag agatggcgat tcgcgcgtat
     7321 caagctatcg attgtgccgg tatggctcgg gtggattttc ttctgaatag aaacacaaac
     7381 caaatctatc tcaatgaggt aaataccatc ccgggtttca ccaagatcag catgtagccc
     7441 aaattgtggg aagccagcgg gatgagttac cgggaattga tcaacgaatt ggtcaatctg
     7501 gcatttgaac gaaaagcgga gagaaaccgc agtgaaagga aatattcagc ctgaaatgaa
     7561 aaagaaagat catgctgagc ctttgaaaag gtcagatatc ctgaggcaaa aacgccagga
     7621 agaacagaat gaatggctgc acacggtcaa ttcccgtgcg gagcatatgg cttcgcgccc
     7681 tgcgttcgag gctttagagg aaaatcagca tgaataccgc ccgttgaacg attctcagaa
     7741 tccacttgcg cacattgcca tcgataacaa agagtatgcg acccctcgta tccgtctgag
     7801 ctggcgcatt ctcagtggag cgatggctgt cattagtctt gttttattga tcagtgcctg
     7861 gcgttcacca gattatcatg tcagtgacgt tgacattcaa ggtatccatc gtatatccaa
     7921 agaagagatc gcagcagcac tgaatatcaa ccagcagcgc gtctttgcac tttcaccaac
     7981 agtgatcgag caaaccatca ccgaagattt tccggaattg tatgacgtga aagcgtccat
     8041 tacgtttcca gcaaaggtag aaataaacgt taaagaacgc cagccatatc tcacctggaa
     8101 ttatcagggg aaaagcatat ggatagatac agatggatac ctgctcccgc aaagagggga
     8161 tgcagagatc accctcacca ttgaatccac ggaaaaaccc cctttctaca ttcctgaaga
     8221 cagggtcata ctgaaaggcg aaaaacgact gcacaaaaca gtggtcgcga aaggagagaa
     8281 cgacacactc gctttattca acgtgtatca aaagatagag ccaattacct atcgagccat
     8341 caaagatctc aaccaactgc tgccagaaca ggatctcatc ttgtacgatg ctcgcagagg
     8401 gttaggttgg aatgatcctc gcgggttcaa agtatatata ggttctaccc tgcaagacat
     8461 cactgcgaag atgaacatga tcgaagagat cgtaaaaacg cttatcaaag ccgccgttgc
     8521 acccacgctg atcagtgtgg aacaaataaa tgcaccgtac tatcgactgg attagtgaaa
     8581 atggaagaag agatcatcgt aggcattgat atcggaacga ccaaagtttg caccctcatt
     8641 gcacgcgtgg aggatgataa tgcattttgc atcctgggcg tcggcattga gcgttctgat
     8701 ggcatgcgta aagggatgat catcgatctt caaaaaacca ccaatgccat cgccaaatcc
     8761 atcgaaaaag cacaacgttc atccggttat gagatcacct ctgccctggt gagcattgcc
     8821 ggttcccacg tctcttcaat caacagccgt ggggcagttg gcattaccaa cggcgtagtc
     8881 agccagatcg acatcaaccg cgcactcgaa gctgcccagg tagttgccat cccacacgac
     8941 cgcgaagtgc tgcacgtcat ccagcgcggt tttaccattg atgggcagga tggcatcaat
     9001 gaaccccttg gaatgcacgg ctatcgattg gaagttgaaa cccatatcat caccgcttcc
     9061 gctgcatccg ttggaaacat tcgaaaatgt gttgaagcag ccggcgtgaa gatcaaagcg
     9121 ttcgtgctga atccactggc ttctgccgaa gtggtattga gcgaaactga gcgccagctt
     9181 ggtgtgatgg tttgcgatat cggtggagga acaaccgacc tggctgtgta cattaacaac
     9241 aatgtctggt acacgacggt tatttctgta ggcggtgacc tggtcacttc ggatattgcc
     9301 tatggattga gattaccact gcccgatgca gagaaagtga aaattgcata cgggcgtgca
     9361 aaagaatcgc tgaccagtgg caatgaaaca ttcagtatcc gatcattcgg tgaagaagaa
     9421 gccaccgaat tccgatacgc ggatctcgcc tatatcatcg aggcgcgtac cgaagagata
     9481 tttcagttcg tcttgcagga gttaaaacgc tccggttacg acggactttt accggcagga
     9541 gtagtactga ccggaggatc aagtctatta cctgggatcc gtgatctggc aagcgacgtg
     9601 ctgggtttac cagtgcgcgt tgcccaaccg gacgacctta ctggacttgt ggatcaattg
     9661 tactcaccag catattccac cagtgtggga ttgttgaagt gggcaggtat cttagcgcgt
     9721 gcggtcaaac gatctacccg tacacgcgga ttaccgccta ttggggcact ggatcgcgtg
     9781 aaagatttta tcaaacgctt cctgccacag taaacaatag gaaaaggagt ttgatgcaga
     9841 tcggaggaga aaaaatggaa acaaattcgt atcaacaagg tgagtcattt gcacgcatca
     9901 aggtaattgg agtaggaggt ggtggttgca atgctgtcaa ccgcatgatc tccgaaggaa
     9961 tacgcggcat tgaatttgtc tcgatcaaca ccgatggtca ggctttgtta ttttcaaaag
    10021 ctgagacccg ggtgcgcatt ggagataaag tcactcgtgg aagaggtgcg gggggcgttc
    10081 ctgaagtggg tcgcaaagct gccgaagaat cttcagaaga actttaccag gttctcaagg
    10141 gcagtgatat ggtctttgtc accgccggaa tgggtggcgg taccggcacc ggagctgctc
    10201 cgatcatcgc ccagattgct aaagaagtag gcgctttgac cattggggtc gtcaccaagc
    10261 ccttcacctt tgaaggcgta caacgcatac aagcggcaga ggtgggcatc accaagctca
    10321 aagagcaggc tgatacactg attgtcattc ccaatgatcg cttattgcag attgtcgaca
    10381 aacgagcgtc gcttgaagag gcattttccg cagccgacga tgttttgagg cagggtattc
    10441 aaggcatatc cgaactcatc actgttccag gtcgtatcaa tcttgacttt gccgatgtgc
    10501 gcaccatcat gtcagaaggt ggtgcagcgc tgatggcggt tggtcgtggc agtggtgaaa
    10561 accgtgcacg cgaggcggct gaaagagcca tttcgagcaa ccttctggat ataaccatcg
    10621 atggcgctca tggcatcctc ttcaacgtga ccggtgggcc agacctgacc ctcttcgaag
    10681 tgaacgaagc ggcggctatc atcaaagaga c
//
LOCUS       DAOSDS010000074        10692 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2361_length_10692_cov_5.217637, whole genome shotgun sequence.
ACCESSION   DAOSDS010000074 DAOSDS010000000
VERSION     DAOSDS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2361_length_10692_cov_5.217637"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLL88_08535"
     CDS             <1..443
                     /locus_tag="PLL88_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01651.1"
                     /translation="ALLLVLFCFPGIPHIYYGDEIGLGGAQDPDCRRPMMWDESQWDL
                     RLYEHFKALITLRKNHPALVHGNFLPLQSFNHMLAFKRIYQNEELIVVINHRFPIKNI
                     SINTCSKKAVWEAIYPHGNFLYSSNSMIEVENVLPYQAMILMGR"
     gene            complement(644..2455)
                     /locus_tag="PLL88_08540"
     CDS             complement(644..2455)
                     /locus_tag="PLL88_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01652.1"
                     /translation="MFKMKKRALFLILFVLISSIFLIPEISLSLNQAKILLFQTILGF
                     FALAVALLLMETLFPGKKNLLVAVGLVGGLILMARLLFYYAGGPISWDELYYMYLSMF
                     PKQESSLLNRYFHIYLQRFFFFLTNWDPFSGAKIFWTFCILATAGFTFYNTYLLIPSE
                     KESTRIFGGIFSLLVYFAFPYILDYPGVTYADYTSMMLCSIFIFLYIQVRKNPQIVFF
                     LMGLLFLFSIKTKEIGLCLAVFIFDKKPYANPSNIKSDIKLACFFLLGISIGILFLAI
                     NDHFLLNDFLFSLRISNWHTLLSFNLHQRTLYDLIDLFGTLVRSNILYLILFTIYIEG
                     ILEKRNKLETPHYFLWAYIITVFAFHLVSTIGGTLITVIRYFVVLAPALGILCAHIVC
                     LIEDAVDKKTVLKLFLYLTACFVINISISFIASTLGWERDLFLNRIFLPMLMLISLFF
                     FFQEKSWTRSIQAGLLGLILFCMLPSKLIALHNRDVENAFERRFQPFAENVDELIDSS
                     KMSLFVSSDVYDSYEILGRDRESNSWMYALLFRQDVNLDQFSYDRFSYENFAEGSFTS
                     AFLSSNDWNSLNMNQKNAIESTYLVRETEKYIFLSVE"
     gene            complement(2544..2615)
                     /locus_tag="PLL88_08545"
     tRNA            complement(2544..2615)
                     /locus_tag="PLL88_08545"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2581..2583),aa:Gly,seq:tcc)
     gene            2757..3644
                     /locus_tag="PLL88_08550"
     CDS             2757..3644
                     /locus_tag="PLL88_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOJ01653.1"
                     /translation="MRVKEFLLRIGVRKHTLRDYFLVIVGASVQAFGMRLFLIPAQLV
                     SGGVSGAAQIIHFFSGFPIGLLVIIGNIPLYLIGWRHLGGLKFALRTLFAVLVFSLLT
                     DLLPYVLPIQSITHDILLNTLYGAVLLGAGLGLVYLGRGTSGGSDILGRILNYRFGFS
                     ISAAYMLTDSLVVLASGFAFGWEKALYGLIVIYMSGLVAEMVSEGSSVVRTALVITEK
                     PDEIAAEVMEELERGVTYLYGKGAYTGNDRTILYCVVSRPEVSIIKGIISEIDPEAFV
                     VIGQANEALGEGFRPLLKK"
     gene            complement(3654..4322)
                     /locus_tag="PLL88_08555"
     CDS             complement(3654..4322)
                     /locus_tag="PLL88_08555"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HOJ01654.1"
                     /translation="MNTGWLGVVFGIAMLDWYAIWTDHEKLNWFTKPATMLALLLWLG
                     LTSGFSGKLLWFGLAILFGLLGDILLLLPRKFFLPGLVAFLIGHLAYVAGFWFDLATL
                     TVQHFALVLVIGVILFLVAPALLNGMRGKDFAKKLRAPVMAYMLIISLMVFCAASTLF
                     SAEWKPTASGLAVSGALLFFCSDTMLAYREFVNTFPKARFWVRVTYHLAQIALVAAAV
                     VNFA"
     gene            complement(4319..5245)
                     /gene="era"
                     /locus_tag="PLL88_08560"
     CDS             complement(4319..5245)
                     /gene="era"
                     /locus_tag="PLL88_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOJ01655.1"
                     /translation="MAEIIQQSFRSGFVSIIGRPNVGKSTLVNALMGQKIAAVSPKPQ
                     TTRARQLAILSDEDAQIILMDTPGMHHPIHTLGRAMNVIVEKTLLDSDVILWLVDVSQ
                     PPQPEDRLIADFFRSVKHLPPVVLGLNKMDLLGKKEREVRRKEFLDLYPEIADVLPFS
                     ARDHSGLDELLQKLKAMLPVGSAYFPEEQVTDYQERQIAADLIREAALLFLQQEVPHA
                     IAVRVDEFSERESGAAYIAATLFVEKESQKGIVIGKGGARLKKIGSLARKSIEEMSGR
                     SVFLQLRVKVSENWRDNVTFLRRMGYEAVEKK"
     gene            complement(5260..5799)
                     /locus_tag="PLL88_08565"
     CDS             complement(5260..5799)
                     /locus_tag="PLL88_08565"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOJ01656.1"
                     /translation="MKDSRRQACMAYIENDNIGPIELYATAKGICKCKFSSLGSFERN
                     LLGQNDHLDATAAQILSDAICQINAYLSGELVRFELAIDWQGISPFAKQVYQAAMEIP
                     FGEVRTYGEIALAIHKPAAPRAVGQALGANPISLIIPCHRVIGSDRHLHGFSAPRGLE
                     TKAWLLQHEGHHIRENRLV"
     gene            6111..6668
                     /locus_tag="PLL88_08570"
     CDS             6111..6668
                     /locus_tag="PLL88_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOJ01657.1"
                     /translation="MNNAQKTYAIEIDSVKRDLRLFEVKPGLKIAILNILGDTELVLA
                     CAKALAKKLDPLKYDVLVTAESKSIPLAHQLSVETQKPYVVLRKDYKVYMGDAIKAET
                     VSITTGKPQILILDEKDRALIKGKKVAIVDDVISTGSTLEGMRHIIEKAGGNITIQAA
                     IFTEGDEGHWKEIIALGNLPVFIEK"
     gene            complement(6675..7862)
                     /gene="galK"
                     /locus_tag="PLL88_08575"
     CDS             complement(6675..7862)
                     /gene="galK"
                     /locus_tag="PLL88_08575"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOJ01658.1"
                     /translation="MSTANPHDLVLTQMPDAGLLVSAPGRVNLIGEHVDYNMGIVLPA
                     TVDRRVWLAVQALQEPILSVHTLDLGENVEIDLDNLDARVDRRGKPLPLWALYPAAVA
                     WMLQTRGFQVRGLKACIASNLPMRAGLSSSAAMEVVFALAFQSLADLQMDRMQLARLC
                     QMAESSFIGVHCGLMDQFAVSHGVARHALYFDTRSLAWQPIPLPPETVLVVADSGKQR
                     ELAHSAYNERQRQCQQAVAILKEYVPAVQSLRDVDMAIYDQNERHLPKVIAKRARHVI
                     EEIERVKRSAVYLANDDATSFGKIMFEGHRSLRDLYEVSCAELDFLVEKAATISGCLG
                     ARLTGAGFGGCTVNLVQKTQAVHFCQQLQLAYQDHFSLELKTYVCQADRGAYVEKIEK
                     AVV"
     gene            complement(7881..8849)
                     /locus_tag="PLL88_08580"
     CDS             complement(7881..8849)
                     /locus_tag="PLL88_08580"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOJ01659.1"
                     /translation="MATQITVLGLGKTGISVGLALEGAKKSVTRIGFDEDAALKSLAK
                     KKKSFDDYPANITDAIKTADIVVLDIPVNKLRTVFVAIASGLKQNSIVVNMSAMAKLP
                     SHWAAELLPEYVYFINAIPAFSYESLQDVDKDPANARAELFAKSMLFLAGDAELRKEV
                     IAVVVDLAVLLGATPCFTDLDELEGLIANVILLPELAAAALAASTMLQPGWDDSQKLA
                     GNIYNLALKPLELVNETDDFGISIFQNKHNVLPILEKYISVLQHLQQLIEKDDYASLS
                     DVLYDILLTRQEWLKKRSLGKWDQFLTSSIPLKQDALKRFSSFSSK"
     gene            complement(8851..9501)
                     /locus_tag="PLL88_08585"
     CDS             complement(8851..9501)
                     /locus_tag="PLL88_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ01660.1"
                     /translation="MNLFNMTPAVFVARILALLISLTFHEFSHAWVAVYFGDETPRWA
                     GRLTLNPFKHLDPLGSLMLLLVGFGWAKPVPVNAFTLKRKNPAALMFVALSGPFSNFL
                     LAVLAAMPLRFGLVNPVVITSSLLPSLFEFLMYFLFINLGLMLFNLLPIPPLDGEQVL
                     AFILPGKLAYFYENIRPYGSFILLAVIVIGQFTGFNLISKVLQPAINGMAAFLLGV"
     gene            9534..9749
                     /locus_tag="PLL88_08590"
     CDS             9534..9749
                     /locus_tag="PLL88_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HOJ01661.1"
                     /translation="MRYNAGMLFELEKGDVVRLRKEHPCGGTTWLVTRLGADIGLECQ
                     TCKRRIMLTRRELMKRMKENLGKEKVE"
     gene            9790..10290
                     /gene="tsaE"
                     /locus_tag="PLL88_08595"
     CDS             9790..10290
                     /gene="tsaE"
                     /locus_tag="PLL88_08595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOJ01662.1"
                     /translation="MPILDESTLEFFSRSPEQTRRVGARLGSLLQAGHTLCLNGDLGA
                     GKTTLVQGIAQGWGSPDPVTSPTFVLVNHYRRVDGQQIAHLDAYRLSDALEAEDLDLE
                     YLLENGPLIVEWPLRVLSALPNTHIWIDLQHISEQQRMLLIKPVGSAYKNIVLQLRKQ
                     IFSELS"
     gene            10287..>10692
                     /gene="tsaB"
                     /locus_tag="PLL88_08600"
     CDS             10287..>10692
                     /gene="tsaB"
                     /locus_tag="PLL88_08600"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOJ01663.1"
                     /translation="MILAIDTSTQWLSVALYDAEQQVFLFEKTWRATRRHTIELAPAI
                     TNLLQEVGTTIQDVDTLAVATGPGSFTSLRIGLAFSKGIVLANRINLIGVPVLALVGH
                     WELAAALIVMERVGKAIRAPARDAIISHAARGM"
BASE COUNT         2960 a         2724 c         2502 g         2506 t
ORIGIN      
        1 ccgcattact ccttgtgctg ttctgttttc ccggcattcc acacatctat tacggtgacg
       61 agatcgggct ggggggagca caagacccgg attgccgtcg ccctatgatg tgggatgaat
      121 ctcaatggga tcttcgtcta tacgaacatt tcaaagccct gatcaccctg cgcaaaaacc
      181 atccggctct tgtgcatggt aactttctcc ccttacagtc tttcaaccac atgctcgctt
      241 ttaagagaat ctatcaaaac gaagagctta tcgtagtgat caatcacaga tttcccatta
      301 aaaatatttc tatcaacact tgtagcaaga aagccgtatg ggaagcaata tatccgcatg
      361 gtaatttcct ctattcgagc aattccatga ttgaagtgga aaatgtgttg ccctatcagg
      421 caatgatttt aatgggaaga taagacgacc gcttatttct aaaagagcat ttgccagaac
      481 cgctcagcaa caattctcca tccccttcga ggagatatta aaacaaaaga gcgggcgatg
      541 ggattcgaac ccacgaatat cagcttggaa ggctgatagc tatcaacagg aaaattttgg
      601 acccacttta taagaagcaa cagatggaaa caaatatccc tgcctattct actgataaga
      661 agatatactt ctctgtttca cgaactaaat atgtgctctc aattgcattc ttttgattca
      721 tgtttaatga attccaatca ttactcgaaa gaaatgcgga tgtaaaggat ccctcggcaa
      781 aattttcata tgaaaatcta tcgtaggaga attggtctag attaacatct tgacgaaata
      841 aaagcgcata catccaacta ttagattccc tatcgcgtcc aagaatttca tagctatcgt
      901 aaacatcaga tgaaacaaac agactcatct ttgatgaatc tattaattca tcgacatttt
      961 ccgcaaatgg ttgaaagcgt cgctcaaaag cgttctcaac atcccggttg tgcagggcaa
     1021 tcaacttaga aggaagcata cagaataaga tcaatcccaa taatccagct tgtatacttc
     1081 ttgtccatga tttctcctga aagaaaaaga acagagaaat gagcatcaac atgggcagga
     1141 atatccgatt caagaacagg tctctttccc agcccagagt actcgcaatg aacgagatgc
     1201 tgatatttat tacaaaacat gctgtaagat atagaaacaa ttttaaaact gttttcttgt
     1261 cgacggcatc ttcaataaga caaacaatat gagcacataa aattcccagt gcgggggcaa
     1321 gaaccacaaa ataacggatc actgtaatta atgtgccgcc gatagtacta acaagatgga
     1381 aagcgaacac agtaataata tatgcccata aaaaatagtg tggtgtctca agcttgttac
     1441 gtttttctaa aataccctct atatagatcg taaagagaat caggtacaaa atgttactcc
     1501 gtactaatgt accgaataga tcaatgagat cataaagggt tcgctgatgt agattaaaag
     1561 agagtaaggt gtgccagttt gaaatccgaa ggctgaacaa aaaatcattt aataaaaagt
     1621 gatcgtttat tgctaagaaa agaattccaa tactgattcc caaaagaaag aagcaagcaa
     1681 gttttatatc acttttgata ttggatggat ttgcatacgg ctttttatca aaaatgaaga
     1741 ctgctaagca caatcctatc tccttggttt ttatagaaaa gaggaataaa aggcccatta
     1801 gaaaaaaaac aatctgaggg ttcttccgca cttggatgta aagaaagata aatattgagc
     1861 acagcatcat actggtgtaa tcggcgtagg ttacccctgg ataatcaaga atataaggaa
     1921 aggcgaagta gaccagcaaa gaaaagattc cgccaaaaat cctggtgctc tctttttctg
     1981 aaggaattaa caagtaggtg ttgtagaatg taaagccggc tgtagcaagg atacaaaaag
     2041 tccaaaaaat ctttgctcct gaaaaaggat cccaattggt aaggaagaag aaaaaccgct
     2101 gcaaataaat atgaaaatag cggtttaaaa ggctactttc ttgcttggga aacatcgaga
     2161 ggtacatgta atacagttca tcccatgaaa tcggtccccc tgcgtaataa aacagcaacc
     2221 ttgccatgag aataagccca ccaaccagac caactgcaac cagaagattc ttcttgccag
     2281 gaaagagagt ttccatcaag agcagagcaa cggctaaagc gaaaaatcct aatatagttt
     2341 gaaagagcaa gatttttgcc tgattgagtg aaagggaaat ttcaggaata agaaaaatgc
     2401 tcgatatcaa aacaaaaaga atgagaaaaa gcgccctctt tttcatttta aacataaatt
     2461 ctccttataa gtacattgtt gattataaat attttttttt cagatttacc ctcttttgaa
     2521 attctactta cttaaaacaa aagagcgggc gatgggattc gaacccacga atatcagctt
     2581 ggaaggctga cgccttacca cttggcgacg cccgcgtgga ttaaagattg tacccatcat
     2641 ccactcgcac gtcaagagaa acgccacgcc gcatcacttt cctcagtcgc ataggatgaa
     2701 aaaagcgact gattcgacta caattaagcc gattccatat tgggaatacg gttcctatgc
     2761 gtgtgaaaga gttcctcctc cgaattggcg ttcgcaaaca tacgctgcgc gattacttcc
     2821 tggtcattgt gggagccagc gtgcaggcgt tcggcatgcg cctgttcctt atccccgctc
     2881 aattggtgag cggcggggta agcggtgccg cgcagatcat ccactttttc agcggcttcc
     2941 ccatcggtct cctggtgatc atcggcaaca tccctctcta cctgatcggc tggcggcacc
     3001 tggggggttt gaaattcgca ctgcgcacgc tctttgcggt gctcgttttt tctctcttga
     3061 ccgatctttt gccctacgtg ttacccatcc aaagcatcac ccatgatatc ttgttgaaca
     3121 cgctgtatgg cgcggtcctt ttgggagcag gcttgggtct tgtgtatctg ggacgcggca
     3181 cctccggcgg ttcagatatc ttggggcgca tcctcaatta tcgcttcggc ttctccattt
     3241 ctgccgccta tatgctcacc gattcgctgg tggtattagc cagtggtttt gccttcggct
     3301 gggaaaaggc gctgtacggt ttgatcgtca tctatatgag cgggttggtg gcggagatgg
     3361 tctctgaagg ttccagcgtg gtgcgcactg ccctggtgat caccgaaaaa ccggacgaga
     3421 tcgcagccga agtgatggag gagctggaac gcggggtcac ttatctgtac ggcaaaggcg
     3481 cctacaccgg caacgatcgc accatcttgt actgcgtggt ttcgcgacca gaggtaagca
     3541 tcatcaaggg catcatcagc gagatcgatc ccgaggcgtt cgtggtgatt gggcaagcca
     3601 atgaggcatt gggggaaggg ttcagacctc ttcttaaaaa atgaatatgc gccttacgca
     3661 aaattgacga cggctgcagc aaccagtgcg atctgcgcca ggtggtaggt cacccgcacc
     3721 caaaagcggg ctttcggaaa agtattcaca aattcacggt atgccagcat ggtatccgag
     3781 cagaaaaaga gcagggctcc tgaaacagcc agccccgaag ccgtgggttt ccattccgca
     3841 ctgaagagcg tggatgctgc acagaatacc atcaggctga tgatgagcat gtatgccatt
     3901 accggcgccc ttaatttttt cgcgaagtct ttgccgcgca ttccattcaa aagggcggga
     3961 gccaccagaa acaagatcac cccaatcacc aataccagag cgaaatgctg taccgtcagg
     4021 gttgccaggt caaaccaaaa tccagccaca taagccagat gcccgatcag aaatgccacc
     4081 aaaccgggca gaaagaattt gcgcggcagc aaaagtaata tatcccccag caacccgaac
     4141 aggatagcca gcccaaacca aagcagcttg cccgaaaaac cagatgtcaa tcctaaccag
     4201 agcagcagcg ccagcatggt tgccggtttg gtgaaccagt tcaatttttc atggtccgtc
     4261 cagatggcat accaatccag catagcgatc ccaaacacca cccctaacca gcctgtattc
     4321 attttttctc caccgcttca tatcccatgc gccgcaaaaa tgtaacgtta tcgcgccagt
     4381 tttcgctgac tttcacacgc agttgcaaaa aaactgaccg cccgctcatc tcttcgatgg
     4441 atttgcgcgc caggctgcca atctttttca gcctggctcc acctttgcca atgacgatcc
     4501 ccttctgtga ttctttttcc acgaagaggg tggcagcgat ataagccgca ccgctttcgc
     4561 gctcgctgaa ttcatccacc cgcaccgcga tggcatgcgg cacttcttgc tgtagaaaca
     4621 gcaaagctgc ttcgcgaatc aggtctgccg caatctggcg ttcttgatag tcagtgacct
     4681 gctcttcagg aaaataagcc gagccaaccg gtagcatggc tttcaatttt tgcagcaatt
     4741 catccaatcc gctgtgatcg cgagccgaga acgggagaac atccgcaatt tcaggataca
     4801 ggtccaggaa ctcttttctc cgaacttccc gttctttttt ccccaacaga tccatcttgt
     4861 tcaaccccaa cacaacgggg ggcagatgtt tgacagatcg gaaaaaatct gcgatcagcc
     4921 ggtcttccgg ttggggaggt tggctgacat ccaccagcca gagaatgaca tccgaatcca
     4981 gcagagtttt ctccacaatg acgttcatcg cccgacctaa ggtatggatc ggatgatgca
     5041 tgccgggagt atccatcagg ataatctgcg catcctcatc gctcagaata gccaactggc
     5101 gcgcgcgggt ggtttgcggt tttggggaaa ccgccgcgat tttctgcccc atcaacgcat
     5161 tcaccagggt tgacttacca acgttggggc gccctataat ggaaacaaaa ccagagcgga
     5221 aactctgttg aataatttct gccatgcaga aattataaat cagaccaggc ggttctctcg
     5281 gatgtggtga ccctcatgct gcagcagcca ggctttggtc tccaagccgc gcggagcaga
     5341 aaagccatgc agatgccggt cgcttcctat tacccggtgg cagggaatga tgagagagat
     5401 gggattggca cccagcgctt gcccgaccgc cctgggcgca gccggcttat ggatggcgag
     5461 tgcaatctca ccataggtgc gcacctcgcc aaatgggatc tccatggctg cctgatacac
     5521 ctgcttggca aaaggagaga tgccttgcca atctatcgcc agttcaaagc ggaccaactc
     5581 acccgaaaga tacgcgttga tctggcaaat cgcgtctgat aaaatctggg cagccgtcgc
     5641 atccaggtga tcgttctgcc cgagcagatt cctttcaaaa ctccccagag aactaaattt
     5701 gcacttacaa attcctttgg cagtagcata gagttctatt ggtccgatat tgtcattttc
     5761 gatgtaagcc atacaagcct gccttcgact gtctttcatg actatttttt gatccaagat
     5821 cacctcgcac agacttcttg gaccaacccc ttttggttaa gtatagccga aagaggaaca
     5881 aaggtatctt aaaaatgaaa atcgagcgta gggtaagtgt agggtaagcg ttgggcaagc
     5941 gtcaagcaga aaaacagcga attctttatt atagtcacaa gggcgacttc tcttcttctc
     6001 ctccttataa gagagccagg gctgatatca ctggctctct attaatttaa ttatccaacc
     6061 atcaattcgt gatacactca attcgccata gcggttttca ggaggaaacg atgaacaacg
     6121 ctcaaaaaac ctatgcgatt gaaatcgata gcgtaaaacg cgacctgcgt ttatttgagg
     6181 tgaaacccgg gctcaagatc gccattctca atatcctggg cgataccgaa ctggttctcg
     6241 cctgcgccaa agctctcgcc aaaaaactgg atcccctcaa atatgatgtt ctggtcaccg
     6301 ctgaaagtaa gagcattccg ctcgcccacc agttgtcggt agaaacccaa aaaccctatg
     6361 tggttttaag gaaggactac aaggtctaca tgggcgatgc cataaaagcc gagacagttt
     6421 ccatcaccac cggaaaacct caaatcctca tcctggatga aaaagaccgc gcgctgatca
     6481 agggcaagaa ggttgccatc gtggatgatg tcatcagcac tggttccacc cttgagggca
     6541 tgcgccacat catcgaaaaa gcgggaggaa acataaccat tcaggcagcc attttcaccg
     6601 aaggcgatga aggtcactgg aaggagatca tcgcattggg aaacctgccg gtctttatag
     6661 aaaaatgaaa gcggtcacac gaccgctttt tctatctttt ccacataagc gccgcgatcc
     6721 gcctgacaga catacgtttt taattccaga gaaaaatgat cttggtaagc cagttgcaat
     6781 tgctggcaaa aatgaaccgc ctgcgttttc tgcaccaaat tcaccgtgca cccgccaaaa
     6841 cctgcaccgg tcaacctggc gcccaggcag ccggatatcg tagcagcctt ctccaccaaa
     6901 aaatccagct ctgcgcagct cacctcatac agatcacgca acgaacgatg accttcgaac
     6961 ataatcttac cgaagctggt tgcgtcatca tttgccagat aaacggcaga acgtttcacc
     7021 cgctcgatct cttcaataac atggcgggcg cgtttggcga taacctttgg cagatggcgt
     7081 tcattctgat cgtaaatcgc catatccaca tcacgcagcg attgcacggc agggacatat
     7141 tctttcaata tggcaaccgc ctgctggcat tgccgctgcc gctcgttata ggctgagtga
     7201 gccagttcgc gctgctttcc cgaatcagcc accaccagca ccgtttcagg cggcagaggg
     7261 ataggttgcc aggcgaggga acgcgtgtca aaataaagag cgtggcgcgc caccccgtga
     7321 ctgaccgcaa attgatccat caaaccacaa tgcacgccga tgaaactgct ttctgccatc
     7381 tggcagagcc gggcgagctg catgcgatcc atctgcaaat ctgccagaga ctggaaagcc
     7441 agggcaaata ccacttccat ggcagccgaa ctgctcaacc ctgcacgcat gggaaggttg
     7501 gatgcaatgc atgccttcaa tccacgcacc tggaaccctc tggtttgcag catccatgcc
     7561 accgccgcag ggtacaaagc ccacaaaggg agcggtttgc ctctccggtc cactcgggca
     7621 tccagattat ccagatcgat ctcaacattt tcacccaggt caagagtatg gacactcagg
     7681 attggttcct gcaacgcttg aaccgccagc cagacccgcc tgtctacggt tgcaggcagg
     7741 acgattccca tgttgtaatc cacatgttcg ccgatcaggt tcaccctgcc gggggcagaa
     7801 accagcagcc cggcgtcagg catctgtgtt agaacgagat catgggggtt cgctgtgctc
     7861 atgatgaatt caaccggggg tcattttgac gaaaaactcg aaaagcgctt tagcgcatcc
     7921 tgtttcaatg gaatgctgct tgtaaggaac tgatcccatt tccccaggct gcgtttcttg
     7981 agccactcct ggcgggtcaa caaaatatca tacaaaacat cactcaagct cgcgtagtca
     8041 tctttttcga tgagctgctg caggtgttgc aaaacggaga tatatttctc caaaatcggc
     8101 aggacattgt gtttattctg gaaaatgctg atgccgaaat catccgtttc attcaccaac
     8161 tccagcggct tcagcgccag attgtagata ttcccagcca gtttttggct gtcatcccag
     8221 cctggttgca acatggtcga agcagccagc gcggcagccg ccaactctgg cagcaagatc
     8281 acgttggcga tcaatccctc cagctcgtcc aggtcggtaa aacaaggggt ggcacccaac
     8341 agcactgcga gatcgaccac cacagcgatc acttctttgc gcaattctgc gtcgccagca
     8401 aggaacagca tgctctttgc gaaaagctct gcccgggcat tggcaggatc tttatccaca
     8461 tcttgcaagc tttcataact gaaagccgga atggcgttga tgaaataaac gtattccgga
     8521 agcagttctg ccgcccagtg gctcggcaat ttcgccatgg ctgacatatt cacaacgatg
     8581 ctgttttgtt tcagaccaga tgcgatggca acaaacaccg ttcgcaattt gttgacaggg
     8641 atgtcaagca caacgatatc agccgttttg atcgcatcag tgatattggc gggatagtcg
     8701 tcaaaacttt ttttcttctt cgccagactt tttaatgctg catcctcatc aaagccaatc
     8761 cgcgtcacgc tcttttttgc cccttccaga gccaatccaa ctgagatgcc ggtctttccc
     8821 agccccaata cggtaatttg tgttgccatg tcagactccc aagagaaatg cagccattcc
     8881 attgatcgca ggctgtaaca ctttgctgat caagttgaag ccagtgaact gcccgatcac
     8941 aatgaccgcc aataagataa aggatccata aggacggatg ttttcataaa aatatgccag
     9001 ttttccaggg aggatgaacg ccaaaacctg ctcgccatcc agcggcggaa tcggcaataa
     9061 gttaaaaagc atcagaccca gattgatgaa caagaagtac atcaaaaatt cgaaaaggct
     9121 gggaagcaat gagctggtga tcacaaccgg gttcaccaag ccaaagcgca gcggcatagc
     9181 tgccaaaaca gccaggagaa aattggaaaa tggaccagac agagccacga acatcagcgc
     9241 cgccggattc ttccgcttga gcgtgaaagc gttgaccggc accggtttcg cccagccaaa
     9301 tcccaccagg agcagcatca aactccccaa tgggtcaaga tgcttgaacg gatttagcgt
     9361 cagcctgccc gcccagcggg gtgtttcatc tccaaaatag actgccaccc aggcgtgtga
     9421 aaattcatga aaggtgaggg aaataagcag ggctaaaatg cgtgcgacga acacggcggg
     9481 ggtcatgttg aataaattca tattggtttt cccattcgtg aagcgattat atcatgcgat
     9541 ataatgctgg catgttgttc gagctggaaa aaggagatgt ggtacgcctg cgcaaggaac
     9601 atccttgcgg tgggacaacc tggttggtta cccgcctggg cgccgatatc gggttggaat
     9661 gccagacctg caaacggcgc atcatgctca cccggcgcga gctgatgaaa agaatgaaag
     9721 aaaatctcgg aaaagaaaaa gtggaataga aatccgtttt taccccgctc atgcgataag
     9781 gttaaggaaa tgccaatttt agatgaaagc acgcttgaat tcttcagccg cagccctgaa
     9841 caaacgcgac gggtgggagc ccgactgggt tcgctcctcc aggcaggtca tacgttgtgt
     9901 ttgaacggcg accttggcgc aggcaagact acgctggttc aaggcatcgc gcagggttgg
     9961 ggctcgcccg acccggtcac cagcccgacc ttcgtactgg tgaatcatta tcgcagagtg
    10021 gatggtcagc aaatagcgca tctggacgct taccgccttt ctgatgcgct ggaagccgaa
    10081 gacctggacc ttgaatatct gctggaaaat ggtccgctga tcgtggaatg gcctttacgc
    10141 gttttgagcg ccctccccaa cacacacatc tggatcgatc tgcagcacat cagcgaacaa
    10201 cagcgcatgt tgctgatcaa accagttggc agcgcataca agaatatcgt cttacaatta
    10261 agaaaacaga ttttttcaga gctatcatga tcctggcaat tgacacttcc acccaatggt
    10321 tgagcgtggc gctgtatgat gcagagcagc aagtctttct cttcgagaag acctggcgtg
    10381 caacgcgccg ccacaccatc gaactggctc ccgccattac caacctgctg caagaggtgg
    10441 ggacaaccat ccaggatgtt gatacactag cagttgctac gggccccggc tctttcacca
    10501 gcctgcggat cgggttggct ttctctaagg ggatagttct ggcaaaccgc atcaatctca
    10561 tcggggtgcc ggtgctcgcc ctggtcgggc actgggagct ggccgccgcc ctgatcgtca
    10621 tggagagggt ggggaaggcc atacgggcac cggcccggga cgccatcatc tcccacgccg
    10681 ccaggggaat gg
//
LOCUS       DAOSDS010000075        10489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2410_length_10489_cov_5.073797, whole genome shotgun sequence.
ACCESSION   DAOSDS010000075 DAOSDS010000000
VERSION     DAOSDS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10489
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2410_length_10489_cov_5.073797"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..933
                     /locus_tag="PLL88_08605"
     CDS             292..933
                     /locus_tag="PLL88_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOJ01664.1"
                     /translation="MKRSSSVERTTNKKPAFRFSETMCTFQDIKQEIVEGKIWNIPNL
                     IMLVRLVLGIAVINFLKDPHGADPLLVIPFVVIMFFLDFLDGALARKLKQQTLFGKTF
                     DPFVDKLLIVYGILSLFKLVEIPTTLSIPFFSTHIIMVAGSVFLMITHRALPAVRSYG
                     AISNVLAVIAAICYWLGLIKIGKKILLLAIVSGYALIIDYARRISWKKGTVHE"
     gene            complement(968..2152)
                     /gene="gguB"
                     /locus_tag="PLL88_08610"
     CDS             complement(968..2152)
                     /gene="gguB"
                     /locus_tag="PLL88_08610"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ01665.1"
                     /translation="MSFIKNIKKTLSENIRDYGMFIALFAIMLFFTITTKGIFLSPRN
                     ISNLVNQTGYIAVLAVGMTLVIVIRHIDLSVGFLAGFMGAIAAIAMVSWHLPVGVVIP
                     MVLVLGILAGFLTAFLVAKLGIPSFVASLAGWLIYRGALLLVTLKTGTIIIPNATYNA
                     IGNGYIPDLQNLNILPGVHKLTLLIGIFAVIFFIIGEIRDREKKKAYDFEVPSMDMFI
                     LKIIFVSALILGVTRVLAGYNGLSWTFVIVLAVTLIYHFITTQTVLGRYIYAVGGNPE
                     AAELSGINVKTITYIVFGSMGMLAALSGILYSSRLQSATTIAGQLFEMDAIAAAYVGG
                     VSAGGGVGRVTGSIIGAFVMMSLTSGMNLMGIDISSQYIVKGAVLAVAVIFDVATRNR
                     GK"
     gene            complement(2163..3683)
                     /gene="gguA"
                     /locus_tag="PLL88_08615"
     CDS             complement(2163..3683)
                     /gene="gguA"
                     /locus_tag="PLL88_08615"
                     /inference="COORDINATES: protein motif:HMM:NF040905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOJ01666.1"
                     /translation="MDNTILEMQHITKEFPGVRALDDVCFSVTAREIHCLVGENGAGK
                     STLMKILSGVYPYGEYEGEIIFNGTVQKYANISDSKKAGIAIIYQELALVPELTVYEN
                     IFLGHEIKSGLVIDWNETIKEATQMLRKVGLDVNPTVKVKELGLGKQQLIEIAKALSM
                     DVKLLILDEPTSALNETDSENLMNLLRDLKKHGVTSVLISHKLKEVIGIADRVTVLRD
                     GKTICTLDASKGEVKENVLIKHMVGREINNVYPRRNAKIIDEKVFELKGWNAYAPKIG
                     RDILKDININVRKGEIVSLAGLMGSGRTELALSLFGNSLRYHVTGEMFVNGKQVHFKH
                     PEHAIKAGLAYVTEDRKGNGLILIQDVKQNITLANLRAIAENGVVNRNEEVVVSKEYT
                     NSLNIKTPSIEQKVLNLSGGNQQKVSVAKWLFVRPQVLILDEPTRGIDVGAKYEIYMI
                     MNRLVEEGMSIIMISSELPEILGMSDRIYVVSSGRIAGELLAKNATQEKIMQLATD"
     gene            complement(3781..4923)
                     /gene="chvE"
                     /locus_tag="PLL88_08620"
     CDS             complement(3781..4923)
                     /gene="chvE"
                     /locus_tag="PLL88_08620"
                     /inference="COORDINATES: protein motif:HMM:NF040907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOJ01667.1"
                     /translation="MFKKYSSILAVLVVFAMLLSACGTAATPEVAEPAAEEPAAESAE
                     LAVGIVLPTKDEPRWIQDETRFADALTAAGYNVEILFSQGDSAKEKTNVESLISKGVK
                     VIIICPQDGSAAAAAAEEARAAGVKVISYDRLILDTDAVDYYVTFDSIAVGAQQAQYL
                     VDHATGTGNPLYLYAGAASDNNAFLFFQGAWEVLQPKIADGTFVIKNSSEAVALQDKL
                     ALTREEMSKIIGQVTTNWDFAVAKNLAEANLTAAPAADKGDVFILAPNDGTARSIADV
                     FAVDPDVTSYYVTGQDAEQASVQYIIDGKQSMTVWKDVRNLVNDAIAAAVALIEGEEP
                     ASRGSYNNNVIDVPAIQSTVTTVDKDNVKETLIDSGYYDASLFTGL"
     gene            complement(5239..6864)
                     /locus_tag="PLL88_08625"
     CDS             complement(5239..6864)
                     /locus_tag="PLL88_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ01668.1"
                     /translation="MQTLLNVQHISKSFGTLPVIQNVNFSVNAGEVVGLTGSTGSGKS
                     VLMMMLAGLYEPDFGHVFFKEKRLNWPFSAKNLGIGVIHQRSTLVEQFDVISNIFLGN
                     EIGKPDYMGILRRLDHHEMQEKAMQLLNRLDIQVNSLHEIVYNLSGEQRQMVSIARVL
                     TFPASLIIIDEPTVSLSYPNQQKLLELIQDWRQKGVAVLFSSNNLDHLFAVTDRIIVL
                     QSGSITADLHTDETNREEVVNYLLGTTKNQHVTPTIWDFNSYDTVREYTEKLRYHQIL
                     LEKDLAAEGSLNRQLAEQLAAQLQALDQTNLALRDAQRRLLSEREEERKHLARELHDQ
                     IIQDLLSVNYDLEEIAMRQTISTNLEHDLATVRQNIRELVGNLRLICGTLRPPTIDSL
                     GLEAALQSYLHDWHQRTGIEFSVEIDENLSRLPESIELSIFRIVQEGLNNVWRHAEAS
                     HVTVALRHTSPRALMVSIQDDGKGLPDDLDLTELSTHGHYGLMGISERVSLLGGRMHL
                     ENGGKRGSVLMVEIPHPRVEAKTKKKREDLLLR"
     gene            7037..8089
                     /locus_tag="PLL88_08630"
     CDS             7037..8089
                     /locus_tag="PLL88_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ01669.1"
                     /translation="MDITRVLLADDHVIVRSGIRRMIEQIPKMEVAGEAENGPQVLTL
                     LRSESVDLLLIDVTMPDFEPIKTISAIRRLYPDIKILVVSAYDDDYYVKGLLNAGVNG
                     YHMKDQPLNDLRTAIERVMAGERWISHTLINKLVEKSIPATPPAALTTRQRTLLRYLL
                     QGLDNKTIAENLGLSIKTVENHLTRLYRQIEVQSRLEAVNFAMRNPHILGIPGYQAGQ
                     EDGETDPLIPRNISILVVDDNARYRKQLLRMIGRVNPRTTIYEADSIQETNQIIRKIV
                     PQLALVDVVLGDEDGIACVRQIKALHPNSRIVLISAYPDREFHRQGLQSGAVAFLDKR
                     NLDMPTLRQVIEDISN"
     gene            complement(8166..10235)
                     /locus_tag="PLL88_08635"
     CDS             complement(8166..10235)
                     /locus_tag="PLL88_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01670.1"
                     /translation="MALETIKTIFQKYGYIGLFLIALLVFLVYPNVGIYDWDKEVLYT
                     AYIKTSILEYRQLPLFLWNSAGLAGYPAVDQSAFFAANPETMLFTPFLPLLFFLSPVV
                     FLKLLVVLNAVAGLVGVEVLAKKLRWQPAQLRVFSALFFLSPIVFQHVAIGYLPWINL
                     YLIPWLLYFALMDDLLPRSVGCGMVLALVLLQGGLHVFVWLAFFTTFYLVSSALIQKS
                     WNQLLGVPLTFLSAIGMAFPRFHLSLQTFSSFSQRFFAGYSLSAFFRWALVPPFFTPA
                     SMDDIEYFIESYIDGVPYWDGALFWGLLIILTLFLPFFFANRYRRNKQGYEGSQGSAL
                     AVGCASALLLVFSFDGLYEALITFVSGLLRLPALEGMEKYPFRFAIPAYFGFAFVIAA
                     DWVDLPVFIHEAIAGVYEVLKKLARVFRTLCGKLRRGRRVFAWLAGVLAALVFASFAA
                     RPALLGWINRHISLAHAGQGADWLAQRMQRASSVPLQSYVSKAAALYGYGQRFLLIAT
                     LLFALLWLFGLLRFSGKAKEKPGIRCSRLPAVLEELLVVPLLLAFGMWWRVALATPQN
                     TVAFFRMNAPQIIVDASQDVPEVEITSFSPGALHLSVTDAVGNSIIVLSDVPARDVRF
                     LEISPGSASLVDHDDRLGIQVQQNGEIEIYVDHTAITIPMLIAVVSWLLAAGMLLRGQ
                     KRSDHHG"
BASE COUNT         2887 a         2747 c         2372 g         2483 t
ORIGIN      
        1 tcccctgcgt tttgctctgc gggcattggt tcgccttgtt tcgaacaggc acccaccatg
       61 agcccgatga gcacacatag cacaactttt ccaaagtgct ttttcatttt tctccttttc
      121 tcttgagttt ttgatccact tagagagtgt aaaaaaattg taacaagaaa ccacaatatg
      181 taaaactatc gttataatca caaaaacaaa tttttaacga aacattctca taaagctcaa
      241 aactatgccc atgcacttta atagaaagaa tcatacagaa aggggtattt gatgaaaaga
      301 agttcctcgg ttgagcgcac aaccaacaag aagccagcct tccggttcag tgaaaccatg
      361 tgcacgtttc aagatatcaa gcaagagatc gtcgagggaa agatatggaa catacccaac
      421 ctgatcatgc tggtgcggct ggttttaggg attgcagtga tcaatttttt aaaggaccct
      481 catggcgcag atcccttgct ggtgattcca tttgttgtga tcatgttctt tttagatttt
      541 ctcgatggag cattagccag aaaattaaag caacaaaccc tttttggaaa aacttttgat
      601 cccttcgtag ataagctcct catcgtttac ggaatcctca gcctcttcaa gctggtggaa
      661 attcccacca ccctaagcat ccctttcttt tccacgcaca ttatcatggt ggcaggttca
      721 gttttcttaa tgatcaccca tcgcgcgtta ccggcagtgc gttcctatgg ggcaatctcc
      781 aacgtgctgg cggtcattgc agcaatatgc tactggctgg ggttgatcaa aatcggcaaa
      841 aagatcctgc tgttggctat cgtaagcggg tatgcgctaa tcattgatta cgcaagacga
      901 atcagttgga agaagggcac agttcatgaa taaaaagagg gagtcatttg ctccctctcc
      961 atctgcttta tttcccacgg ttgcgcgtgg ctacatcgaa gatcaccgcc acagccagca
     1021 cagcaccttt cacaatatac tgagaggaaa tatcaatgcc catcaagttc ataccgctgg
     1081 tcaacgacat cattacgaac gcaccaatga tcgaacctgt tacacgaccc acgcctccac
     1141 cggctgaaac gccacccaca taagccgcag cgatggcatc catttcaaag agctgcccgg
     1201 caatggtcgt agcggattgc agtcgtgacg aatacaagat gccggacaga gctgccaaca
     1261 tacccatgga accaaacacg atataggtaa tggtcttcac attaataccg ctcaattcag
     1321 cagcttcggg gttgccacca accgcataaa tgtatcttcc aagaactgtt tgcgtggtaa
     1381 tgaaatggta aataagcgtt accgccagca cgatcacaaa cgtccaagaa agaccattat
     1441 agccagccag aactcgggta acccccaaga taagcgctga cacaaagatg atctttaata
     1501 tgaacatatc catggacggc acttcaaaat cgtaggcttt cttcttttcg cgatccctga
     1561 tctctccaat aatgaagaaa atgaccgcaa aaatgccaat cagcaacgtc aatttatgca
     1621 ctcccggaag aatgtttaaa ttttgaagat ccggaatgta accattgccg atcgcattgt
     1681 acgtcgcgtt ggggataata atggtccccg ttttcaatgt caccagtagc aacgctccac
     1741 gatagatcag ccaaccagcc agagaagcca cgaatgatgg aatgcctaat tttgccacca
     1801 aaaaagccgt aagaaacccc gcaagtattc ccaagaccaa caccatgggg atcaccacac
     1861 ccacaggcag atgccaggaa accatcgcaa tggctgcaat ggcacccata aatccagcca
     1921 aaaaaccaac tgaaagatca atatggcgaa tgacaatcac cagggtcatc cccaccgcca
     1981 aaacggcgat atatccggtt tgattcacca aattgctgat attgcgggga gaaagaaaga
     2041 tacccttggt cgtaatagtg aaaaacaaca tgattgcaaa caatgcaatg aacattccat
     2101 aatcgcggat attctctgaa agtgtttttt tgatgttttt gatgaatgac atagctctct
     2161 cctcaatctg tcgctagttg cataattttt tcttgggtcg cattttttgc caataattcg
     2221 ccggcaatac gcccggagga aaccacataa atgcgatcgc tcatacccaa aatttcaggg
     2281 agttctgatg agatcatgat gatactcatg ccctcttcca ccaatctatt catgatcata
     2341 tagatttcat atttcgctcc aacatcgatg ccacgggtgg gttcatctaa aataagcacc
     2401 tgcggacgga cgaacagcca tttcgctact gacactttct gttggtttcc gccactaaga
     2461 ttgagaactt tttgttcaat gctgggtgtt ttgatattga gcgaattggt atactctttg
     2521 ctcacaacca cttcttcatt acggttcaca acaccattct ctgcaatagc acgcaaattc
     2581 gccagcgtga tgttctgctt gacatcctgg atgagaatca gtccgttccc cttgcgatct
     2641 tcggtcacat acgccagacc agcttttatg gcgtgttcag ggtgtttaaa atggacttgc
     2701 ttgccattca caaacatttc acccgtcaca tgatacctga gtgaatttcc aaacaaactg
     2761 agcgccagct ccgttctgcc cgaccccatc agacctgcca gactgacgat ctcgcctttg
     2821 cgaacattaa tgtttatatc ttttaaaata tctcttccga tcttcggggc ataggcgttc
     2881 caccccttta actcaaacac cttttcatca ataattttgg catttcgcct tggatacaca
     2941 ttattgatct cacgacctac catgtgcttg atcagcacgt tctccttgac ctcacccttg
     3001 gatgcatcca aggtacagat cgtctttcca tccctcaaga cggtcacccg atctgctatt
     3061 ccgatcacct ctttcaactt atgcgagata agaacagagg tgaccccgtg ttttttcaga
     3121 tcacgcagaa ggttcatgag gttctcgcta tccgtctcgt tcaaggcaga ggtgggttca
     3181 tccaaaataa gcagtttcac atccatgctc aaagctttgg caatttcgat gagttgttgc
     3241 ttccccaatc caagttcttt tactttcacc gtgggattga catcaagccc gactttgcgc
     3301 agcatttgag tggcttcttt gatggtttca ttccagtcga tcaccaagcc gctcttgatc
     3361 tcatgcccaa ggaaaatatt ttcatacacc gtcaattccg gcaccaacgc cagctcttga
     3421 taaatgattg cgatacccgc ttttttgctg tcactgatat ttgcgtattt ctgcacagtt
     3481 ccattgaata tgatctcccc ctcatactct ccgtacggat acacgccgct gagaatcttc
     3541 atgagggtcg atttgcccgc accgttctcc cctaccaggc aatggatttc tctggcagtc
     3601 acgctaaaac aaacatcatc caatgcccgt acacccggga attctttggt gatatgctgc
     3661 atttcgagaa tcgtattatc cataataaac tccggctgtt gattactccc tctgaataaa
     3721 attttacagg aatagcagtg ggaaaaccac cactattcct gtattggtta gactctggtt
     3781 ttatagcccg gtgaataagc tggcgtcgta gtatccagaa tcgatcaatg tctctttgac
     3841 gttatccttg tcaacagtgg taacagtaga ttgaatggca gggacatcga tgacattatt
     3901 gttataggag ccacgagaag caggttcttc accctcaatc agggcaacag ccgccgcaat
     3961 ggcatcgtta accaggttgc ggacatcttt ccacaccgtc atggattgct tgccatcaat
     4021 gatgtactga acagaagcct gttcggcatc ctgcccggtc acatagtagc tggtcacatc
     4081 cgggtccacg gcaaagacat cggcgataga acgtgcagtg ccatcatttg gcgcaaggat
     4141 gaacacatcg cccttatcag ccgcaggtgc agcggtgagg ttggcttcag ccaggttctt
     4201 cgccacagcg aaatcccagt tggtggtgac ctgaccgata atcttgctca tttcttcacg
     4261 ggtgagggcc agtttatctt gcagagcgac agcttcgctg gaattcttga tcacaaacgt
     4321 gccatcggca atcttgggtt gcaacacttc ccaggcaccc tggaagaaca agaacgcatt
     4381 attatcagag gcagcgcccg catagagata gagcggattg ccagtgccgg tcgcatgatc
     4441 gaccagatat tgggcttgtt gcgcacccac tgcgatgcta tcgaaggtga cataataatc
     4501 aaccgcatcg gtatcgagga tcaggcggtc ataagaaatg accttgacac cagccgcgcg
     4561 tgcttcctca gcggcggctg ccgcagcaga accgtcttgg gggcaaatga tgataacttt
     4621 gacacccttg gaaatcagag actccacatt ggttttctct ttagcagaat caccctggct
     4681 gaagagaatt tctacattgt agcctgcagc agtcaatgca tcggcaaagc gggtctcatc
     4741 ctgaatccag cggggttcat ccttggtggg aagaacgata ccgacagcta attcagcact
     4801 ttctgccgct ggttcttcag cagccggttc cgcaacttcg ggggtggcag ccgtaccgca
     4861 cgctgaaagc agcattgcaa agacgaccaa aactgccaga attgacgaat acttcttgaa
     4921 cattttctac ctccaaatat ttgtgtttta ttctcaaaaa aatttattac aatcgttttc
     4981 ccttccacct ccctatcact tatcagatga tcgtttatcg tcttctcttt ccggaaagac
     5041 aggtttgtcg agatgagaac aagatcaaaa tgcagcaagc tatcggctga ctcattttgt
     5101 aatgtactgt agagaaattg tgccccccta ccatctcgga gaaacacaaa gtattcccat
     5161 accttcaatt ggttaagaat acattaatca gaaatgggat tttaagaaaa aacccttgtt
     5221 tttattgggg ggtttcccct atcgcaaaag gagatcttct ctttttttct tggtcttggc
     5281 ttctactcga ggatggggta tttccaccat cagaaccgac ccacgcttgc cgccattttc
     5341 cagatgcatc ctcccaccca gcagagaaac ccgttcactg atccccatca acccataatg
     5401 cccatgagta gatagctcgg tcagatcaag atcatccggc agacccttgc catcgtcctg
     5461 gatagaaacc atcaaagcgc gcggagaagt atggcgcagt gccaccgtaa catggctggc
     5521 ttctgcatga cgccaaacat tgttcaatcc ttcctggaca atgcggaaaa tagaaagttc
     5581 gatcgactcg ggcaagcggc tgaggttttc atcaatttcc accgaaaatt cgatgccggt
     5641 gcgctggtgc caatcgtgca ggtacgactg cagggctgct tccaaaccga ggctgtcgat
     5701 ggtgggggga cgcagtgttc cacaaatgag gcgtaaattt ccaaccaatt cgcgaatgtt
     5761 ttgccgcacc gtagccaggt catgctccag attggtcgaa atggtctggc gcatggcgat
     5821 ctcctccaga tcgtaattga cacttaacaa atcttgaata atttggtcgt gcaattcgcg
     5881 cgccaggtgc ttgcgctcct cttcacgctc cgagagcaag cgacgctggg catcccgtaa
     5941 agccaggttg gtctgatcca aagcctgcaa ttgcgccgcg agctgctcag ccaattgccg
     6001 gttcagtgat ccctctgcag ccagatcttt ttcgagcaaa atttgatgat accgcaattt
     6061 ctccgtgtat tcgcggacgg tgtcataact attgaaatcc caaatggtgg gggtgacatg
     6121 ctgattcttg gtagtgccca gcaaataatt caccacctct tcacgattgg tctcatcggt
     6181 atgcaggtca gccgtaatag aaccagattg cagcacgatg atgcggtcgg tgaccgcaaa
     6241 aaggtgatcg agattattgc tgctgaagag gaccgccaca cctttttgac gccaatcctg
     6301 aatcaactcc aataactttt gctgattggg gtagctcaag gacacagtcg gctcatcgat
     6361 gatgatcaga ctggcaggaa aagtgagcac acgcgcaatg gagaccatct gacgctgctc
     6421 accggagaga ttatagacga tctcgtgcag tgagttcacc tgaatatcaa gccggttcaa
     6481 caactgcata gccttttcct gcatttcatg atggtccaga cggcgcagga tccccatgta
     6541 atccggttta ccgatctcat tgccgagaaa gatgttgctg atgacatcga attgttccac
     6601 cagcgtggag cgctgatgga tcaccccaat gccaagattc ttcgcagaaa atggccaatt
     6661 caaacgcttc tctttgaaaa aaacatgccc aaaatccggt tcatataaac cggcaagcat
     6721 catcattaac acagattttc cagagccggt gctgcccgtc agcccaacca cttctccggc
     6781 gttgaccgaa aaattcacat tttgaatgac aggcagggtg ccaaatgatt ttgaaatgtg
     6841 ttgaacattc aatagcgttt gcattttctt tactggggag aaacgtacca aagggtctta
     6901 cgaataagcg caaacatcaa ccgagccttc ccacatttgt ctttctcctc ttcggttttt
     6961 cattataccc gatatggtga ggggaaattc tcatccattt tcattcgggc gggagaaaca
     7021 gtggtataaa gtgcatatgg acataacacg tgttctgctg gcggatgacc acgtcatcgt
     7081 gcgctcaggc attcgcagaa tgatcgaaca gatacccaaa atggaagtgg ctggagaagc
     7141 cgaaaacggt ccgcaggttt taaccctgct gcgttctgag tcggtggatc tgttactgat
     7201 cgacgtaacc atgcccgact ttgaacccat caaaacgatc agcgccattc gccgattgta
     7261 tcctgatatc aagatactgg tcgtcagcgc gtatgacgac gattattatg tcaagggatt
     7321 gctcaatgcg ggcgtgaatg ggtatcatat gaaagatcaa ccattgaacg acctgagaac
     7381 cgcaattgaa agggttatgg ctggagaacg atggatctcc cacacgctca tcaataaact
     7441 tgtagagaaa agcattcctg ctaccccgcc ggcagccctc accacgcgcc agcgcacact
     7501 gttgcgctac cttttacaag gtctggataa taaaaccatt gcagaaaatc tggggctgag
     7561 catcaagaca gttgaaaacc acctcacccg gctgtaccgt caaattgaag tgcagagccg
     7621 cctggaagca gtgaacttcg ccatgcgcaa cccgcacatt ctggggatac ccggatatca
     7681 ggcagggcag gaagatggcg agacggatcc cttgataccg cgcaacattt ccatactggt
     7741 agtggatgat aatgcccgct accgcaaaca attattgcgc atgatcggca gggtaaaccc
     7801 gcgcactacc atctatgaag cggactccat ccaggaaacg aatcagatca tcagaaagat
     7861 cgtcccgcaa ttggcgctcg tggatgtggt tctgggagat gaagatggga tcgcctgtgt
     7921 gcgccagatc aaagctttgc accccaacag ccgcatcgtt ctgatcagcg cataccctga
     7981 tcgcgaattt caccggcaag ggctgcaatc aggcgcggta gcttttttgg ataaacgcaa
     8041 tctagacatg cccaccctgc gtcaggtgat cgaagacatc agcaactgat tcatagccct
     8101 tttgttttgc gccgaccagg gcagaacatt ccatccagag ttctatcgct ggctaatcgt
     8161 tccggctatc catgatggtc ggatcgtttt tgccctcgca ggagcattcc ggctgccaag
     8221 agccagctca caaccgcgat cagcatggga atggtgatcg cggtatgatc tacatagatc
     8281 tctatttccc cattctgctg cacttgaatc cccaggcgat cgtcatgatc aacgagtgaa
     8341 gcgcttcccg gcgatatttc cagaaagcgc acgtcacgag ccggcacatc gctcagaacg
     8401 ataatggaat tacccaccgc atccgttaca gagagatgga gggcaccagg ggaaaatgac
     8461 gtgatttcca cctccggcac gtcctgactc gcatctacaa taatctgagg agcgttcatt
     8521 cgaaagaaag ctacggtatt ttgaggggta gccagcgcca cacgccacca catgccaaaa
     8581 gccaacagta aaggaacgac cagcaactct tccaggacag ccggcagacg actacagcgg
     8641 atgcctggct tctccttcgc cttcccagag aaacgcaaaa gaccgaaaag ccatagaagc
     8701 gcgaagagaa gcgtagcaat cagcaaaaag cgttgaccat agccatacag agcggcggct
     8761 ttggagacat agctttgcag aggcacactg ctcgccctct gcatgcgctg cgccagccaa
     8821 tccgctccct gcccggcgtg cgcaagcgaa atatgccggt ttatccagcc gagaagcgca
     8881 gggcgcgctg caaaacttgc aaacaccaat gcagccagaa ccccagccaa ccaggcgaaa
     8941 acccgccgcc cacgccggag tttgccacac aaagttcgaa agactcgcgc cagttttttg
     9001 agcacctcat aaacgccggc aatggcttca tgaataaaaa ccggtaagtc tacccaatcg
     9061 gcagcgatca caaaagcaaa tccaaagtac gccggaatgg cgaagcggaa gggatatttt
     9121 tccatgcctt ccagcgcagg caagcgcaac aaccccgaaa caaaggtgat caacgcttca
     9181 tacaaaccat caaaggaaaa aaccaacagc aatgcgctgg cacagccaac cgccaacgct
     9241 gaaccttgcg agccctcata gccttgtttg tttcttcgat agcggttcgc aaaaaagaaa
     9301 ggcaggaaca aagtcaagat gatgagcaga ccccagaaaa gggcgccatc ccaataggga
     9361 acgccgtcaa tatagctctc aatgaaatat tcaatatcat ccatgctggc gggggtgaag
     9421 aaagggggca cgagcgccca gcggaaaaac gcggacagcg agtagcctgc gaagaatcgt
     9481 tgcgagaagg aggagaaggt ttgcaacgaa agatggaaac ggggaaaagc catgccgatg
     9541 gcagacagaa acgtcagcgg tacgcccagc aattgattcc aactcttttg aatgagcgcg
     9601 ctgctcacga ggtagaaggt cgtaaaaaaa gccagccata caaacacatg cagcccaccc
     9661 tgcagcagca ccagcgcgag taccatcccg catcccacag agcgggggag aagatcatcc
     9721 atcaatgcaa agtagagcag ccaggggatg agatacagat tgatccaggg caggtagccg
     9781 atggcaacat gctggaacac tatgggggac aaaaagaaaa gtgcagaaaa cacgcgcagt
     9841 tgagcaggtt gccagcgcag ttttttcgcc agcacctcca cacccaccaa tcctgcaaca
     9901 gcgttcaaca ccaccagtaa tttaaggaaa accacgggtg agagaaaaaa cagtagtgga
     9961 aggaaaggag taaaaagcat ggtttcgggg ttggcagcga agaaggcgct ctgatccacc
    10021 gccggatagc ccgccagacc tgcgctgttc cacaggaaga gggggagctg cctgtattcc
    10081 aagatgctgg tcttgatata agccgtatat aacacttcct tatcccaatc atagatgcca
    10141 acattggggt aaacaagaaa gaccaataac gcgatcagga acagaccgat gtacccatat
    10201 ttctgaaaga tcgtctttat tgtttccaaa gccatttttt cgatcccgca tcccattata
    10261 aaagaaaagc gtgtgaagcg gataagcgtc ccgcaggcaa aaattcgttt tacaattgga
    10321 gagatgacgc gaaaaaggat tttcttctca cttctgatca cactcctgct ggctgcctgc
    10381 cagaccagcg cagaggcgcg acccacgcag gttgatctga ccaatttcac gctcacgccg
    10441 accccggcag ctaccaccac ccccactccg ccgatcctga acgctcccg
//
LOCUS       DAOSDS010000076        10252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2483_length_10252_cov_4.065804, whole genome shotgun sequence.
ACCESSION   DAOSDS010000076 DAOSDS010000000
VERSION     DAOSDS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2483_length_10252_cov_4.065804"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="PLL88_08640"
     CDS             complement(<1..928)
                     /locus_tag="PLL88_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01671.1"
                     /translation="MEKNSFWRKLLAFLLSIVVVAIVFPTIFAVPLEFLIMNESTYSS
                     LVTNEKLLNAGQDAFSDYIAHQLSVAGDGEVVPPVFENTEVLSEGIKAFVTNEWLSQT
                     LNDVSVQLLQFLNFKQPFGIVQIDLSAIKADVLAERDSFVENILNSSAPCNTEEIAQL
                     TSGSLSVQDVPLCNPPTELKSRMVAFISDYVEEFLYKIPQEYQINVEDAIMVNQTNSL
                     FSYSMVRWIFRILPVLFLVLLILIAVCLRKNRAEMRTWIGRLLVTAAALSLVLLLVLL
                     IGSEQFTALFINQTLSAENSAFGTLLLIILQSV"
     gene            complement(1045..1986)
                     /gene="fmt"
                     /locus_tag="PLL88_08645"
     CDS             complement(1045..1986)
                     /gene="fmt"
                     /locus_tag="PLL88_08645"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOJ01672.1"
                     /translation="MQESPENTTIVFMGSPQFAVPILEAIDGKYQVVAAVTQPDKPAG
                     RGKKMEACTVKKYAVSRGIPVLEPVRLRKEPERIEELKQFKADLFVIAAYGQILPQSV
                     LDIPKYGCINVHASLLPRWRGASPIQAAILHGDHESGVTIMKMDAGMDTGPILRQTSI
                     PLVSDENEKSLSEKLRVLGRDLLMEVIPEYLAGTITITPQDDEAATYAPLIKKEDGKL
                     DVEQPVELLERKVRALNPWPGTFLEWNGKQLKVHEVEVISPRMQDVGVRGIHERYPVV
                     QCVGGGLLLKKVQVPGKKMISGRDFLNGVHDWVNGGA"
     gene            complement(2095..2346)
                     /locus_tag="PLL88_08650"
     CDS             complement(2095..2346)
                     /locus_tag="PLL88_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01673.1"
                     /translation="MKAEGFNSGQLENELKKRFIRVQPDENFVRRLSQRLFEKGSISL
                     ESSSKFLYILFLIIFGFLSGYLIVWVLKKVFRTKPKDIP"
     gene            complement(2357..2893)
                     /locus_tag="PLL88_08655"
     CDS             complement(2357..2893)
                     /locus_tag="PLL88_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ01674.1"
                     /translation="MDDVLNKAIKGDLEAFSVLYRENVQRIYAYIYYRTGNKHDAEDL
                     TARVFQRALSHIQKYENKGVPFSAWLYRIAHNLIANWHRDRSRKKEVSIDETPMEISS
                     AEIPESSVIRDQEIVELMGYVRTLPADRQTLLILKFVDDLTNIEIASIMGKSEGAIKS
                     LYHRTLLQLRDRMNEDKK"
     gene            3012..3842
                     /locus_tag="PLL88_08660"
     CDS             3012..3842
                     /locus_tag="PLL88_08660"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HOJ01675.1"
                     /translation="MIHQIWDWKTTDNATMFAQKWMPEKDIHASIILVHGFGEHSSRY
                     EHMADFYCKHHIQVLTFDLRGHGRSAGSRGHIPTQTTFWDDLNEYQKHVKDSLSNKPV
                     FLYGHSMGGMIAITYVLKYKPVFTGVIVTSPLIDTAKPLSDATLKLARLMNKIAPKFA
                     MDSGLDRSGLSRDQRIVDTYNADPFVFGKATTRLGVFLADSKTCILEHASEFNLPLLT
                     MAGSGDRIVSTQEIDKFMQANPKATYKVWNGFYHELHNEPEKEEVFNYTLDWIKQRMR
                     "
     assembly_gap    3958..3967
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4049..7195
                     /gene="gcvT"
                     /locus_tag="PLL88_08665"
     CDS             4049..7195
                     /gene="gcvT"
                     /locus_tag="PLL88_08665"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOJ01676.1"
                     /translation="MNHNHNDFLFREDISSIDPEVQALIKLEEERQVRKLIFIPSEST
                     APWSVRNSLSSVLQNLYAEGYPPEHMGTQNQAEILDYEQQLAFYRRYSDPRYYKGVEF
                     ADIVESLAKRRCAELFCPTFMKPEELFVNVQALSGAPANNAVYQALITPGTTIMGMDL
                     FHGGHLSHGSPVNRSGKLYNAVHYSVDPETEKLNYEQILELAKQLQPKIIICGYSSYP
                     WIPDWLEFRKIADAVGAYMFADISHIAGLIAARVVPSPVGIADVITFTTHKTLCGPRG
                     ACIITKDASIAKKIDKAVFPGEQGGPHVQVFAAMATTFKIASTQKFRDLQKQIVLNCK
                     ALGEQLTKRGLRLSFQGTDSHLLNIDCKSVKGKDGAALSGDLAARILDQVGIVANRNT
                     IPGDTSALKAGGVRMGTPWTTQRGLLEKDMQEIADIIADLFSAIEPYYSAGSKGENLR
                     AKVKFSALEEAKLRIRTIAERAAADLTVEKHGYPFFYFHDDYESTQQKSVSFTIQGKQ
                     VRQFLNYALAEDIEGLNENESMRVTFYVEEKSAHGMLGYLGEEKGYCLTLPSSDAGLA
                     ANWLRALSDGFVYFDQDLLKKLPGPIIIRETAEVGEMKEVSPTAHSSKPFYIHSPSTT
                     ADRKALSEFQWSEKQGTPLKRTCLYEEHIQAGAKMVPFAGWEMPVWYTSVIEEHAAVR
                     NNAGLFDVSHMGVFLAEGVDAQAFLDSVCANEIAALEIGESCYTHFLDTDAHVIDDTI
                     VYHYAHNKYLVVVNASNEEQDWAWFQAVKNSEVLIDRQKPWAIAYGRNVILHNLKDPA
                     AGKGMRVDIALQGPKSRQILCKLDWDKETIARINSLKRTQLCEAALNGTSVIISRTGY
                     TGEKMAFELFLHPDKAPALWNELLEKGKELGLIACGLGARDSLRTEAGLPLYGHEMGG
                     DLNLGVNEAGFSPFVKTHKPWFIGREAYLRSEKDRKGVVVRFRFNQQRVKMAHHGDPV
                     LDERGKVIGTVTSCAIDKEEYLTGQAFVEMKYAPEGSKILIYQNKEALEDVHFSELKS
                     GSRVTLPASATVVSRFMK"
     gene            7226..8458
                     /locus_tag="PLL88_08670"
     CDS             7226..8458
                     /locus_tag="PLL88_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOJ01677.1"
                     /translation="MSTFWDNRFAQRTQRMKGSAIRELLKFTEDPEVISFAGGMPAPD
                     AFPTKEFTKACNQVLKTQSSQALQYGSTDGYLPLREMIARQSKRYGIEVTPENILITA
                     GSQQALDLLGKIFINPGDHIVIENPTYVGALQALNAYGAEYIPVPTDDEGMVTSELEK
                     SLRSSPKFIYVLPNFQNPTGVTLSFERRLQLIELSEKYGVPIIEDDPYGQLRFEGENL
                     PAIEVLDSQMRAQDGCYSGNVIYTSTFSKTLSPGIRLAWVIAPTEVIQKLVLAKQGTD
                     LHTATFNQMVAYEIGKDGFINEHVKYIRKIYKERRDVMLDTLAEHMPEGVKWTHPQGG
                     LFLWLTLPECIDSRELLSEALKFKVAFVPGSSCFADGSHPNTMRLNFSYANPDLINEG
                     IGRLANAIKQFIAKNNQS"
     gene            complement(8455..10170)
                     /locus_tag="PLL88_08675"
     CDS             complement(8455..10170)
                     /locus_tag="PLL88_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOJ01678.1"
                     /translation="MRKEFVSEVKIPRDTVEIYLPDGRIYQGKRGLPVMEFMKILPEW
                     KTPPIVGAVVDGELRELTFPIKKDSHVKPVIMSDSDGSKIYRRSLVFLLQASFEDVFP
                     KLELTVDYSVPSGGYFCTVKNHPPLSENAVKKIEKRMQEIVKKDLPIKREIVKLEEAK
                     EYFRKKGYDDKLQLLKYRKKPSLVLYRLENNRDYHHGYMVPSTGFLTVFALVPVEEGF
                     ILQYPRRKTPTYLLPMPKYTKLRGIFQQYGDWLTSLGIDSVGSLNDAVLNNGIHEIIL
                     VSEALHEQKIAEIATQIANSKPEKKLILVAGPSSSGKTTFSKRLAIQLLTHGIKPFAL
                     EMDNYFVDRERTPLDEEGKPNFEAFEAVNRSLLEKDLLQLMSGVEVQLPNFNFHEGKS
                     EPGEIVQIAKDQVIIMEGIHGLNIALLSKFPARKIFRIYISCLTQLNLDRFNRISTTD
                     TRMLRRVVRDSWARGFTAQRTISLWESVRRGEREYIFPYQENAHAIFNSALVYELAAI
                     KPFAEPLLMQIPFGIDEYREAKRLLAFLNWFLPIEQDFIPDNSILREFIGKSILADFK
                     IWHHH"
BASE COUNT         2739 a         2490 c         2254 g         2759 t
ORIGIN      
        1 tgaccgactg caaaataatc agcaacaacg ttccaaatgc gctgttttca gccgacagcg
       61 tctggttgat gaacagcgct gtaaattgtt ccgaaccgat cagcaaaacc aataaaagaa
      121 caagggatag cgctgccgcc gtgaccagaa ggcgcccgat ccatgtgcgc atttctgccc
      181 ggttcttcct cagacagacg gcaatcaaga tcaacaatac caggaacaac accggcagga
      241 tgcggaatat ccatcgaacc atgctgtatg agaacaacga attggtctga tttaccatga
      301 tcgcatcttc aacattgatc tgatactcct gcggaatttt atacaagaac tcttccacat
      361 aatcagaaat gaatgccacc attctgcttt tcagttcggt tggcggattg cacaacggca
      421 cgtcttgtac ggatagactg cccgatgtca attgcgcgat ctcttctgtg ttacaaggcg
      481 ctgaagagtt caagatattc tcgacaaacg agtccctctc tgccagaaca tcggctttga
      541 tagctgacag gtcgatctga acaattccaa atggttgttt gaaatttaag aactgtaaca
      601 actgtacact gacgtcattc agcgtttggc tcaaccattc gttcgttacg aatgctttaa
      661 taccttcaga taaaacttcg gtattttcaa aaaccggcgg gacaacctcg ccatcgccag
      721 ctacgctcaa ctggtgggca atataatcag aaaaagcatc ttgccctgcg ttcagtaatt
      781 tttcgttggt caccagggaa gaataggtgg attcattcat gatcaagaat tccaatggca
      841 cagcaaagat cgtgggaaat acaatcgcca ccacaacaat cgaaagaagg aacgccagca
      901 gctttctcca aaacgaattc ttttccatat tacagtttct ccttcatgta tttatgcact
      961 caattttctt ctattgtatc aaaatgaccc gctcttttct gcaacaattg tgaatcgaat
     1021 cagtgaacag tcagcacaat atgattatgc tcctccatta acccaatcgt gaacaccgtt
     1081 tagaaaatct ctcccgctga tcattttttt gcctggcact tgaactttct tcaacaacaa
     1141 tcctcctcca acgcactgga caacaggata tctttcatgg atccctctga cccctacgtc
     1201 ctgcattctt ggtgaaatca cctctacttc atgtaccttc aattgctttc cattccattc
     1261 caagaacgtc cccggccacg gattcaatgc gcgcactttc cgttccagca gttccacagg
     1321 ttgctccaca tccaattttc cgtcctcttt ttttatcagc ggcgcataag ttgctgcttc
     1381 atcatcctgc ggagtaatgg tgatcgtgcc agccagatat tcggggataa cttccatcaa
     1441 gagatctctc ccgagcacac gcaacttctc cgataggctc ttttcattct cgtccgaaac
     1501 gagaggaata cttgtttgcc gaaggattgg tcctgtatcc atcccagcat ccattttcat
     1561 gatggtcaca ccgctctcat gatccccgtg caggatggct gcctggatgg gagatgctcc
     1621 cctccagcgg ggtagcagag aggcatgcac attgatacag ccatatttcg gaatatcaag
     1681 aacgctttgc ggaagtatct gcccatacgc agcaatcacg aaaagatcgg ctttaaattg
     1741 tttcaattct tcgattcttt ccggttcctt tcgcaaccgc accggctcca gaactggtat
     1801 gccacgactc acagcatatt ttttcacggt acacgcttcc atctttttgc cacgccctgc
     1861 tggtttatcg ggctgggtaa cagccgccac tacctgatat ttaccatcaa tagcctcaag
     1921 aatgggaacg gcgaattgcg gtgatcccat aaaaacgatc gtggtgtttt ccggtgattc
     1981 ttgcataccc tggttctttc ccttctgata cattagatca caattctgga tgtggttacc
     2041 gagatatgcg gcattgtggt gactagtttt gccctaccca attttgtgac cattttacgg
     2101 aatatctttc ggtttcgtgc ggaacacttt tttcaacacc caaacaataa gatacccact
     2161 caggaaaccg aatatgatca gaaaaaggat atagagaaat ttcgaactcg actccaaact
     2221 gatggagccc ttttcaaaca acctctggct caaccttctc acaaaatttt catccggttg
     2281 gacgcgtatg aatcgctttt tcagttcatt ttccaattga cctgaattga atccctctgc
     2341 tttcatatcc atactcctat tttttatctt cattcattcg atctcgtaat tggagcagtg
     2401 ttcgatggta aaggcttttg atcgcacctt cgctttttcc catgattgaa gctatttcga
     2461 tatttgtcag atcatcaaca aattttaaga tcaataatgt ttgccgatca gcaggaagtg
     2521 tgcgcacata acccatcagt tccacaattt cctggtccct gatcaccgat gattccggta
     2581 tttcagcgct tgaaatctcc atgggcgttt catcaatgga cacctctttt tttctactac
     2641 gatcacggtg ccagtttgct atcaggttgt gggcaatgcg atagagccag gcagaaaaag
     2701 gcacaccttt attttcatat ttctgaatat ggctgagtgc tcgctggaag accctggctg
     2761 tgagatcttc cgcgtcatgt ttgttgccgg tgcggtagta gatataagca tagatacgct
     2821 gaacattctc acgatacaac accgagaaag cctcaaggtc acctttgatg gctttgttca
     2881 gaacatcatc catggaaatt tcttgatttt cgttatttga catataatgt gtactgtttg
     2941 tttaatctta tcatcaatga gttaaaaacc gttgatgatc cgaagggtca tattcatttt
     3001 ggaggcgcta aatgattcat cagatttggg actggaaaac aacagacaat gcaacaatgt
     3061 tcgcacaaaa atggatgcct gaaaaagata tccatgcgtc tattatcctg gttcacggct
     3121 ttggtgagca tagcagtcgc tacgaacaca tggcagattt ttactgcaag caccatatcc
     3181 aggtgctcac ttttgacttg cgcgggcatg gtcgatcagc cggctcacgc ggtcatattc
     3241 ccacccaaac taccttttgg gatgatttga atgagtacca aaaacatgtg aaggattccc
     3301 tttcgaacaa accggttttt ctttatgggc acagcatggg tgggatgatc gcgattacct
     3361 acgtgctcaa atataaaccg gtttttactg gagtgatcgt gacatcgccc ctgatcgaca
     3421 ccgcgaaacc attaagtgat gcaacattga agctggcaag attgatgaac aagattgcac
     3481 ccaagttcgc catggatagt ggtcttgatc gcagtggctt atcgcgcgat cagcgtatcg
     3541 tagataccta taatgctgat ccttttgttt ttggaaaagc cactaccaga ttaggggttt
     3601 ttctggctga ctcgaaaaca tgcatccttg agcatgcttc agagttcaac ttgcccttat
     3661 tgaccatggc aggatcaggc gataggatcg taagcacaca ggaaatcgat aaatttatgc
     3721 aagccaatcc caaggcgact tacaaggttt ggaatggctt ttaccacgaa ctccataatg
     3781 aaccggaaaa agaagaggtc tttaattaca cactggattg gatcaaacag agaatgcgat
     3841 agctattaag agggattaca ggcattctcc aataatcatt acttgacctt ctttctaaga
     3901 aactttaaaa tgatttcagt tatgatgttg ctcatattgc gtattgacac ctgttaannn
     3961 nnnnnnnaga gattaaaaaa cgcttgtctc tttctataag ggcaagtgtt tttttatata
     4021 ttaccttgat cgggaaggat tattaaaaat gaaccataat cacaatgact ttctttttcg
     4081 cgaagacata agctccattg atcccgaagt acaagccctc atcaagctag aagaggaacg
     4141 ccaggttaga aagttaatct ttatccccag tgaaagtacc gctccctgga gcgtacggaa
     4201 cagcctttct tccgttcttc agaatctcta tgccgaagga tacccacccg aacacatggg
     4261 tactcaaaac caggcagaaa tccttgatta cgagcagcaa cttgcctttt accgtcgcta
     4321 ttcagacccg cgatattata agggcgtcga atttgcggat attgtagagt ccctcgccaa
     4381 acggcgctgc gcagaactgt tttgccccac tttcatgaaa ccggaggagc tttttgttaa
     4441 cgttcaagcc ctctccggtg ctcctgcgaa caatgccgtt tatcaagccc tcatcacccc
     4501 cggcacaacc atcatgggga tggatttgtt tcatggtggg catctttcgc atggatcacc
     4561 ggtcaaccgt tccggaaagt tgtataacgc cgttcattat tcggtcgatc cagaaaccga
     4621 aaaactcaat tatgagcaaa tcctcgaact agccaaacaa ttgcagccca agatcattat
     4681 ctgcgggtat tcctcatatc cgtggattcc agattggctg gaatttcgca agatagccga
     4741 tgccgtgggt gcatacatgt ttgcagatat ttctcacatt gccggattga tcgccgccag
     4801 ggtggtcccc tcgccggttg gcattgccga tgtgatcacc tttaccaccc ataaaacatt
     4861 atgtggaccg cgcggcgcgt gcatcatcac caaagacgcg tcgattgcca aaaagatcga
     4921 taaagctgtt ttccccggcg agcagggcgg tccacacgtc caggtctttg cagccatggc
     4981 taccactttt aaaattgcca gcacgcagaa attccgtgat ctgcaaaaac aaattgtctt
     5041 gaattgtaaa gcgctgggag agcagctaac caagcgtggc ttgcgcctgt cctttcaggg
     5101 tacggattca catttactga acattgattg taaatctgta aaggggaagg acggcgccgc
     5161 tttatccggt gaccttgccg ctcgtattct tgatcaggtg gggatcgtag ctaatcgcaa
     5221 caccatcccg ggcgatactt ctgctttgaa ggcgggtggt gttcgcatgg gaactccctg
     5281 gacaacccag cgtggattgc ttgaaaagga catgcaggag atcgcggata tcattgctga
     5341 cctgttctca gccattgaac cgtattatag cgccggcagt aaaggcgaaa acctgcgcgc
     5401 aaaagtgaag ttttcagccc tggaagaggc aaaattgcga atccgcacta tcgccgaacg
     5461 agccgctgcc gacctgaccg ttgagaagca tggctatcct tttttctact tccatgacga
     5521 ttacgagtca acccaacaaa agtctgtgtc attcaccatc cagggaaaac aagtgcgcca
     5581 gttcttaaat tatgccctgg cagaagacat cgaagggctg aatgagaacg aaagcatgcg
     5641 tgttactttc tacgttgaag agaaatccgc acatgggatg ctggggtacc tgggtgaaga
     5701 aaaagggtat tgcctgaccc tgccttcttc tgatgccgga ttggctgcca actggttgcg
     5761 cgcgctttct gacgggtttg tgtacttcga tcaggacctg ttaaagaaac tccccggtcc
     5821 gatcatcatt cgtgaaaccg ccgaggtcgg ggaaatgaaa gaagtctcac ccaccgctca
     5881 ttctagcaaa ccattttaca ttcatagccc ttcaaccacg gctgatagaa aagcattgtc
     5941 agaatttcaa tggagcgaaa aacagggtac acctctcaaa cgaacctgct tgtatgaaga
     6001 gcatatccaa gcgggtgcca agatggttcc ttttgcagga tgggaaatgc cagtctggta
     6061 tacgtccgtc atcgaggaac atgccgccgt caggaacaac gccggtttgt tcgatgtttc
     6121 gcacatgggg gtgttccttg ctgaaggcgt tgacgcccag gcatttctgg atagtgtctg
     6181 tgctaatgaa atagctgcat tggaaattgg tgaatcctgc tatacccact ttctagatac
     6241 cgatgcccac gttatcgacg atacgatagt atatcactat gcgcataaca agtatttggt
     6301 ggttgtgaat gcctccaacg aagaacaaga ttgggcgtgg ttccaggctg taaagaacag
     6361 cgaagtcctt atcgatcggc aaaaaccatg ggcaatcgct tatggtcgga atgtgattct
     6421 tcataacctc aaggatccgg ctgcaggaaa aggtatgcgc gtggatatag cacttcaggg
     6481 tccgaaatct cgccagatcc tctgcaaatt agattgggat aaggagacca tcgccaggat
     6541 caattctttg aaacgcacgc aattgtgtga agcagccttg aacggcacaa gcgtcatcat
     6601 atcgcggacc ggttacaccg gcgaaaaaat ggctttcgaa ttgttccttc accctgacaa
     6661 ggctccggct ttatggaatg agcttctgga aaaagggaaa gaactgggtc tcatcgcttg
     6721 cgggttgggc gcccgagatt cattgcgcac cgaagcagga ttaccacttt acggtcacga
     6781 gatgggcggt gatcttaacc tgggtgtgaa cgaagcgggg ttttcaccct tcgtaaagac
     6841 gcacaaaccc tggttcattg gtcgtgaggc gtacctgcgc tcagaaaaag atcgcaaagg
     6901 agttgtggtt cgctttcgct tcaatcaaca gcgggtgaag atggcgcacc acggcgatcc
     6961 cgtcctcgat gagcgtggaa aagtgattgg cacggtaacg agttgcgcaa ttgataagga
     7021 agagtacctc accggtcagg catttgttga aatgaaatat gccccggaag gcagtaagat
     7081 ccttatctac cagaacaagg aagccttaga agacgtccac ttttctgaat tgaagagcgg
     7141 cagccgcgtt acactgcctg cttctgccac agtagtcagc cgttttatga aatgaacaaa
     7201 atgtacctgt aaggaggaac attccatgtc aacgttctgg gataaccgtt ttgcgcagcg
     7261 gacacaacgc atgaaaggtt ctgcaattcg tgaattgtta aaattcaccg aagatcccga
     7321 agtgatctcc ttcgctggtg gtatgcctgc cccagatgct tttccaacca aagaattcac
     7381 caaagcctgc aatcaggttc tcaaaaccca gagcagccag gcattgcaat atggttcaac
     7441 cgatggatat ctgcccctgc gtgaaatgat cgccaggcaa tccaaacgtt acggaataga
     7501 agtcacaccc gaaaacatcc tgatcaccgc tggttcacag caggcgctgg atctgctggg
     7561 caaaattttc attaatcccg gtgaccacat cgtgattgaa aaccccacct atgttggagc
     7621 tctgcaggct ctcaatgcct atggcgcaga atatataccc gtccccaccg atgatgaggg
     7681 aatggtcact tcagagttgg aaaaaagttt gcgcagcagc cccaaattta tctacgtttt
     7741 gcctaatttc cagaatccca ccggcgtaac gctttctttc gaacgccgcc tgcagttgat
     7801 cgaactttca gaaaagtacg gtgtgccgat cattgaagat gacccctatg gacaactgcg
     7861 cttcgaaggt gaaaatcttc ccgccatcga agtgcttgat agtcaaatgc gcgcccaaga
     7921 cggctgctat tctggcaatg tgatctacac cagcaccttt tccaaaacgc tttcacccgg
     7981 catccgtctg gcgtgggtca tagctcccac tgaagtcatt cagaaacttg ttttagccaa
     8041 acagggaacc gatctgcaca ctgccacttt caatcaaatg gtagcttatg aaataggaaa
     8101 agacggtttc atcaatgaac atgtgaaata tattcgaaag atctataaag aacggcgcga
     8161 cgtgatgctg gacacgcttg cagaacacat gcccgaaggt gtgaagtgga ctcatccgca
     8221 gggtggatta ttcctgtggc tcacactccc tgaatgtatc gattctcgtg aactcctcag
     8281 cgaagcgtta aagtttaaag tcgctttcgt tcccggtagt tcttgctttg cagatggaag
     8341 ccatccaaat acgatgcgat tgaatttttc ttatgccaat cccgatttga tcaatgaagg
     8401 catcggtcgt ttggcaaatg ccatcaagca gttcattgcc aagaacaacc aatcttagtg
     8461 gtgatgccag atcttgaaat cagccaggat tgatttcccg atgaattccc ttaagatcga
     8521 attatcgggg ataaaatctt gttcaatggg gagaaaccaa tttaagaatg ccagcaagcg
     8581 ttttgcctct cggtattcgt ctatcccaaa ggggatttgc atcaacaagg gctcggcaaa
     8641 cggttttatg gcagccaact cgtaaaccag cgccgaattg aaaatggcat gggcattttc
     8701 ctgataaggg aaaatgtact cccgctcccc cctcctgacg gattcccaca atgaaatggt
     8761 gcgctgtgcg gtgaaaccac gtgcccacga atcgcgcacc accctgcgca acatgcgcgt
     8821 atctgtggtg gagatacggt tgaaacgatc aagattgagc tgggtgaggc aagaaatata
     8881 aatacgaaag atcttgcgag cagggaactt ggacaacagt gcaatgttca atccatgaat
     8941 cccttccatg atgatcacct gatcctttgc gatctgcact atttctccgg gttcactctt
     9001 cccttcatga aaattgaaat tagggagctg cacttccaca ccactcatca actgcagcaa
     9061 atctttctca agtaaagaac ggttgacagc ttcaaatgct tcaaaattgg gcttgccttc
     9121 ttcatccagg ggtgtgcgct cgcgatccac aaaataatta tccatttcaa gtgcaaaagg
     9181 tttgatgccg tgcgttaaaa gttggatggc tagacgcttt gagaatgtgg tctttccaga
     9241 agatgatggt cctgcaacca ggatcaactt tttttctggc ttgctatttg cgatctgtgt
     9301 ggcaatctct gcgatctttt gctcatgcag cgcttcagaa accaggatga tctcgtggat
     9361 tccattgttt aagacagcgt cattcaaaga acccacgctg tcaatgccca aactcgtaag
     9421 ccaatcacca tattgctgaa atatccctct caacttcgta tatttcggca tcggcagcag
     9481 ataggttggc gtttttcttc gtggatactg taaaataaat ccctcttcca ccggtaccag
     9541 agcaaacact gtaaggaagc cggttgaagg taccatgtag ccatgatggt agtcgcgatt
     9601 attttcaagc ctgtacaaaa ccagacttgg ttttttcctg tatttcagca attgcagttt
     9661 gtcatcatag cctttctttc taaaatattc tttcgcttcc tccagcttca caatttctct
     9721 tttgatcggc aggtcttttt taacgatctc ctgcatgcgt ttttctatct tcttcacagc
     9781 attttcgctc aaaggggggt gattcttcac agtacagaaa tacccgccgc tggggacaga
     9841 ataatcgaca gtcagttcca atttggggaa cacatcctca aaagacgctt gtagcaagaa
     9901 caccagtgag cgcctgtaaa ttttcgatcc gtccgaatca gacataataa caggtttcac
     9961 atgggaatct ttttttatcg ggaatgtcaa ttcccgcagt tctccatcta caaccgctcc
    10021 gacaattgga ggggtcttcc attctggtag tattttcatg aactccataa ccggaagacc
    10081 gcgctttccc tgatagatac gaccatcagg caaatagatc tccactgtgt cgcgtggaat
    10141 tttcacttca cttacgaatt cttttctcat aggtttttgt tttattgtaa aattctatct
    10201 tgattataat cactggaatc tatctatgga aaattacttt tggcgattga tc
//
LOCUS       DAOSDS010000077        10015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2550_length_10015_cov_4.192169, whole genome shotgun sequence.
ACCESSION   DAOSDS010000077 DAOSDS010000000
VERSION     DAOSDS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10015
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2550_length_10015_cov_4.192169"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="PLL88_08680"
     CDS             <1..670
                     /locus_tag="PLL88_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01679.1"
                     /translation="TPVVSGACGVEYFPVLPDQTWTYRLLQANNSFVESRIWYEDITD
                     NSFVWKQEMDSDPPIASQTNWTCSGDGLISTDFVSTNIPMVMQGMGYDYDYEIETIEF
                     SGIAYPANDQWFVGSQWTGSWVIESDINVENVGLVHATINATLNNTIGAEESVTVPLA
                     TYEEAARVDSTLSLEIKIEAQGMTLPTVKEDYALTSWFVRGVGLVKQISDDIAYSMEL
                     ISLE"
     gene            784..3588
                     /locus_tag="PLL88_08685"
     CDS             784..3588
                     /locus_tag="PLL88_08685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOJ01680.1"
                     /translation="MTSLVAFDLETTGLDPQRDKIIEIGAVKFDGTRILDTYQTLVNP
                     QRAISPEITQLTSITNEMVRSAPLIEETRDEFAAFLGNDPLLGHNISFDLSFLRSNRF
                     VLVNEEVDTYDLASVLMPTASRYNLGSLVEILKIKTQGLQSHRALDDARMTVEVFLRF
                     VQMGDELPLELVREIVDQGKGLSWGGKWFFNQLLRQRIHEPVQAKKVRQEDYGVLFGS
                     LEELALPPLKPKAVLESLNEDEVAAHLEPGGTFSRFMNQFESRSQQIEMARMVANAFN
                     NSQHFMVEAGTGTGKSFAYLVPAALWAIHNQTRVLISTNTINLQDQLIKKDIPDTQQA
                     LGLDLRATVLKGRSNYLCPRRLEGLRFRGPRSREEMRMLAKVLVWLHQGGSGDRGELN
                     LNGGLEWETWSKLSAEDETCGAEACMNRLGGVCPYFRARQSALRAHIIVVNHALLLAD
                     VVTENRVLPDYDYLIIDEAHHLEDASTSALSYKVSNKDVKRSFEELGSPKTGALGNVT
                     AGLQGRVSEEQFSAYHEVLEEITDLAFRADHEFDRVFDSMQTFMEDMREGKEVSEYGQ
                     RVRVELSTTRQPIWSQIEMCWQNASTTLESMLNDLSRLIREISDLDFAQELEVNEDLT
                     ELARLTRRFQETLTNFSSLIYEPEPSNVYWLEIEPRSERFSVNMAPLHIGPLMEKYLW
                     NEKQSVILTSATLTTSGDFDYLRNRLNAETADELLLGSPFDFETSTLLYLPIDMPEPI
                     DRSQYQLWVERTLLRLAKATGGRMLVLFTSYKQLRLTSRSITPLLNKDHIQVFEQGEG
                     PSSNALLETFRAAERAVLLGTRSFWEGVDVPGQALSVLVIVRLPFDVPSDPIITARSE
                     TFENPFQEYQLPEAILRFRQGFGRLIRTKSDRGVVTVLDSRILNKQYGQLFLNSLPPC
                     TQKRGPVEELPAVAARWLDL"
     gene            complement(3639..4325)
                     /locus_tag="PLL88_08690"
     CDS             complement(3639..4325)
                     /locus_tag="PLL88_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOJ01681.1"
                     /translation="MKIQRATLATCPTASGLVVVIDVLRAFTTAGYLFDAGVEEIFLV
                     SQVKEAFALRNQLPDCIIAGEVDGVRVPGFDMGNSPSTINAVVHPAKRVILRTTAGTQ
                     GVILAKEAGVILTTALTNVSATVRYIQNLAPESVTLIQSGLFEDRGWGDEDVACADVI
                     EDMILNKPIYWNSIYQRVRASKAGTHYDGTRIDFPFADLQMTLERDRFHFAMLVERKD
                     GLFCTHPVNS"
     gene            complement(4345..4761)
                     /locus_tag="PLL88_08695"
     CDS             complement(4345..4761)
                     /locus_tag="PLL88_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOJ01682.1"
                     /translation="MDAKVIWKENMSFEGTADSGFKVPLGTVAELGGNNDGFRPLELL
                     AIGIAGCTAMDVISILAKKKQDVRDFEVLVHADRAEEHPKVFTHLTIEYVVSGKGIER
                     AAVERAVELSETKYCSAQAMFKKVAPMELKITVKEI"
     gene            complement(4991..6043)
                     /gene="tsaD"
                     /locus_tag="PLL88_08700"
     CDS             complement(4991..6043)
                     /gene="tsaD"
                     /locus_tag="PLL88_08700"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOJ01683.1"
                     /translation="MEEQSNEKKSIRILGIETSCDETAAAVLEDGSQVLSDVVASQAD
                     LHARYGGVFPEVASREHIKTIYAVVDQALKDAHLDIHELDAIAVTRGPGLAGSLVVGM
                     NMAKGLSLASGIPLIGINHLEGHLYSAWIPLDNPEYSQPTFPVMALIVSGGHSSLVLM
                     QDHLTYTQLGRTLDDAAGEAFDKVARLLNLPYPGGPSIQKAAESGNGQAFQFPRAWLE
                     GSWDFSFSGLKTAVLHTLQELRADGRPVLVENLAASFQTAVVDVLCQKTLAAAREFHA
                     RSILVAGGVSANRALRKAFLDQKEFPVHIPPLKLCTDNAVMIASAGYHRFTHGFKNDL
                     AMDVLPNWSLVTVSKE"
     gene            complement(6051..7289)
                     /locus_tag="PLL88_08705"
     CDS             complement(6051..7289)
                     /locus_tag="PLL88_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOJ01684.1"
                     /translation="MKKDLDAIMQEQGIDALWISGAAQNNPDMVYFTGIHHVSSADLI
                     KVRDQEPLLFYNSMEREEAAKTGLTVQGYDEKYPLRKYAQTTNGDLLQAIALRYKDIF
                     TDLGIQNKKIAIAGRYDLSAFYAVVSAMQELLPGTEFAGCFTEGPIKLARLTKSADEV
                     EHIRKMGQITTEVVGNTADFLCNCRAKHDTLVDKDGNPITIGLVKSYIRRWLAEKGAD
                     NPEETIFSIGRDAGIPHSAGNADDRMELGKTIVFDIFPCEAGGGYFYDFTRTWCLGYA
                     SEEIQKVYQDVFTVHQGMLESLKVNTPFRDYQAATCRMFREFGYETIQENPETTEGYV
                     HSLGHGLGLDVHESPFSGMGAGARDILQPGTVFTIEPGLYYPSKGFGVRIEDTVYLND
                     QGKVEMLADFPYQLVLPLKE"
     gene            complement(7303..8652)
                     /gene="selA"
                     /locus_tag="PLL88_08710"
     CDS             complement(7303..8652)
                     /gene="selA"
                     /locus_tag="PLL88_08710"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOJ01685.1"
                     /translation="MTDRSHFPSIDRLLHLKSTLPLLDTYGHELVVEALRQALDGIRK
                     GGKAFTEEKIIDESEDLLLAWTAPTLVPVINASGVILHTNLGRAPLSKKTCQETENIA
                     ISFNNLEYDLDAGKRGSRSVHAERILTRLSGAEAATIVNNNAAAVLLVLTALAQRKRV
                     LVSRTQLVEIGGGFRVPDVMRQSGAKLVEIGTTNRLHLYDYENALQEPTALVLCAHHS
                     NFKIVGFTSEPSLASICTLAHQKGVLVFHDLGSGALLDTRKYGLPHEPMVQESLQAGV
                     DVVSFSGDKLMGGPQAGIILGKKGLIEKIKKHPLARALRPDKACLAGVSSTLLAYLTG
                     KAEQEIPVWQMLALPAQTISERALQWKNLLDCGDIIEGRSMIGGGSLPEESLPTFLLA
                     LKVKKPDQLLKVLRQQKTPIIARIEEGCVVLDPRTVLAHQEQELLGTLQKILSDWKE"
     gene            complement(8645..9052)
                     /locus_tag="PLL88_08715"
     CDS             complement(8645..9052)
                     /locus_tag="PLL88_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01686.1"
                     /translation="MMKKQEITLGKVLPVGLILTAAGGAGLLLVLFNMQPFLGPRWLL
                     FFFVSLLACGVSLPIITMIQLRFSKKNLTEGVLLRETILFSAYVDLLLWLQLGRVLSN
                     LIMLLLGMGFVLLEIFLRISERAVFFAGGKKDD"
     gene            9094..>10015
                     /locus_tag="PLL88_08720"
     CDS             9094..>10015
                     /locus_tag="PLL88_08720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ01687.1"
                     /translation="MRGILTFLINTLEGALLKKITSTFKEYPQSFQILAGATFIDRVG
                     GTMIQPFYSLYIAQKFGVNMTTIGLLFGIWAVSGVVGNMLGGALTDKIGRKTMLVAGL
                     VLSATTAILMGIVNNLVAFALISTLVGMLSDIAGPARGAMTTDMLPVDKRAEGFGIIR
                     VVGNLAWIIGPALGGLLASRSYMMVFICDAIASIITALIVVWKIPETKPQAGEETEKE
                     TLLQTFKGYGQVVSDKLFMVYVLISMLLIFVYGQMYSTLSVYLNQQHDIPAKGYGWLM
                     SMNALMVVLFQFSVTRGTRKKPPMYMMALAT"
BASE COUNT         2216 a         2645 c         2599 g         2555 t
ORIGIN      
        1 cacgccggta gtgagtggtg cctgcggcgt ggagtatttc cccgtcctgc cagatcaaac
       61 gtggacttac cgcctgcttc aggcgaacaa ctcgttcgta gaaagccgca tctggtacga
      121 agacatcacg gacaattcgt ttgtctggaa gcaggaaatg gatagcgatc cgcccattgc
      181 atcgcagaca aattggacct gttctggtga tggtttgatc tccactgatt ttgtctcaac
      241 caatatcccc atggtgatgc aggggatggg ctatgattac gattatgaga tcgagaccat
      301 tgaattcagc ggcatcgctt atccggcgaa tgatcaatgg tttgtcggct cgcaatggac
      361 gggttcctgg gtgatcgaga gcgatatcaa tgtggaaaat gtggggctgg tgcatgcaac
      421 catcaatgct accttgaaca atacgattgg ggcggaggaa tcggtgaccg ttcccctggc
      481 tacctatgaa gaagccgcgc gagttgattc taccctgtcg cttgaaatca agatcgaggc
      541 gcagggtatg accctgccga cggtgaagga ggactacgcc ctgacctcct ggttcgttag
      601 aggggttggg ttggtgaaac agatttcaga tgatattgcg tattccatgg agctgatctc
      661 tctggaatga ccaacgtttc tggcgatcga gaggcaatcg aacaggattg cctcttttgt
      721 tatgagttgc gattgggttg aaaaatcgtt gcttgttgaa agcctgatga tataatcttt
      781 ttcatgacct ctcttgtcgc cttcgacctt gaaaccaccg gtttggaccc ccagcgcgat
      841 aagatcatcg agatcggtgc ggtgaaattt gatggaaccc gcattctcga cacctatcag
      901 accctggtca atccccagcg tgcaatctcc cctgagatca cgcagcttac ctctatcacc
      961 aatgagatgg tgcgcagcgc tcccctcatc gaggaaacgc gcgatgaatt tgctgctttt
     1021 ctggggaatg atccgttgct ggggcacaat atctcgtttg atctttcctt cttgcgcagc
     1081 aatcgctttg tcttggtgaa cgaggaggtt gacacctacg accttgccag tgtgctcatg
     1141 cccaccgcca gccgttacaa tttaggctcg ctggttgaga ttttgaaaat aaaaacccaa
     1201 ggcctccaat cacatcgcgc gctggatgac gccagaatga cggttgaggt ctttttgcgc
     1261 tttgtgcaga tgggcgatga actgcccctg gaattggttc gcgagatcgt ggatcaggga
     1321 aagggtttga gctggggtgg aaaatggttt ttcaatcaac tgctcagaca acgcatccac
     1381 gaacctgtgc aagccaaaaa agtgcgccag gaggattatg gcgttttgtt cggaagcctt
     1441 gaagaactgg ctttgccccc gctgaaaccc aaagccgttt tggaatcctt gaatgaagac
     1501 gaggtggctg cccatttgga acccggtggt accttctccc gtttcatgaa ccagttcgag
     1561 agtcgctctc aacagatcga aatggcgcgc atggtggcga acgctttcaa taatagccag
     1621 catttcatgg tagaagccgg taccggcacc ggaaaatctt ttgcatatct ggtgcctgca
     1681 gctctttggg cgatccacaa ccagacgcgc gtgctcattt ctaccaatac catcaatttg
     1741 caggaccagt tgatcaaaaa ggatatccct gatacccaac aggcgcttgg cttggatctg
     1801 cgggctactg tgctgaaagg aaggagcaat tacctctgtc ctcgccgtct ggaagggctg
     1861 cgttttcgcg gtccccgttc gcgcgaagag atgcgcatgc ttgccaaggt gctggtctgg
     1921 ctgcatcaag ggggcagcgg tgatcggggc gagttgaacc tcaacggtgg tttggagtgg
     1981 gaaacctgga gcaagctttc tgcggaagat gaaacctgtg gggctgaggc gtgtatgaac
     2041 cgattgggcg gggtatgccc ctactttcgc gccaggcaga gcgcgctccg cgcccatatc
     2101 attgtggtca atcacgctct cctgttggca gacgtggtca cagaaaaccg cgtcttgccc
     2161 gattacgatt acctgatcat cgatgaagct catcatctgg aagatgcttc taccagcgca
     2221 ctgagttaca aggtttcgaa taaagatgtc aagcgtagtt ttgaagaatt gggcagccct
     2281 aaaaccggcg cgttgggaaa tgtgactgct ggcttgcagg gccgggtgtc cgaagagcag
     2341 ttctcagctt atcacgaagt tttggaagag atcaccgatc tcgcgtttcg agctgatcat
     2401 gaatttgatc gcgtgtttga ttctatgcaa acctttatgg aagatatgcg tgagggaaaa
     2461 gaggtcagcg aatatgggca gcgtgtgcgg gtcgagcttt ctaccacgcg ccaacccatc
     2521 tggtcgcaga tcgaaatgtg ttggcaaaat gccagcacca cgctggaaag catgctcaac
     2581 gatctctctc gcctgattcg cgagatatcc gatctggatt ttgcgcaaga attggaagtg
     2641 aatgaggatc tcaccgagct ggcgcgtttg acgcgtcgtt ttcaggaaac attgaccaat
     2701 ttttcttcct taatttatga accggagccg tcaaacgtct attggttgga gatcgagcct
     2761 cgttctgagc gcttttctgt caacatggct cccctgcata ttggtccgct gatggaaaaa
     2821 tatctgtgga atgaaaagca gagcgtcatt ctcacctccg ccacgctcac caccagcggc
     2881 gattttgatt atctgcgtaa tcgcttgaac gccgagacgg cagatgaact gttgctgggc
     2941 tccccctttg attttgaaac ttccaccttg ctgtatctgc ccatcgatat gcccgaaccc
     3001 atagatcgca gccagtatca gctctgggtg gagcgtaccc tgctgcgctt ggcgaaagcc
     3061 acagggggga gaatgctggt gcttttcact tcctacaagc aattgcggct gacttctcgt
     3121 agcatcaccc ctctgttgaa caaggaccat attcaggtct ttgaacaggg ggaaggacct
     3181 tcctcgaacg ccttgcttga aaccttccgc gcagccgagc gcgctgtgct gctgggtacg
     3241 cgttcgttct gggagggcgt ggatgtgcct gggcaggcgc tctcggtgct ggtcattgtg
     3301 cggctgccgt tcgatgtgcc cagcgacccg atcatcaccg ctcgctctga aaccttcgag
     3361 aatccattcc aggaatacca gctccccgaa gccatcctgc gcttcaggca gggctttgga
     3421 cggctcatcc gtaccaaatc ggatcgtggt gtggtgactg tgctggatag ccgtatcctg
     3481 aataaacagt acgggcaatt attcttgaac tccctgcccc cctgcacgca aaaacggggt
     3541 ccggtggaag agttgccggc tgtcgccgcg cgctggctcg atctgtaggt tggttgaaga
     3601 caccctatag cgagatttca cgatgcagta aatacatgct agctgtttac cgggtgcgtg
     3661 cagaaaagac cgtctttcct ttctacgagc atggcaaaat gaaagcggtc tcgctcgagt
     3721 gtcatttgca gatctgcaaa ggggaaatcg atgcgtgtgc catcatagtg ggttcctgcc
     3781 tttgatgcgc gcactcgttg gtaaatcgaa ttccagtaaa tgggcttatt caagatcata
     3841 tcctcgatca cgtcagcgca tgccacatcc tcatcccccc atcccctatc ttcaaataga
     3901 ccgctttgga tcaacgtcac actttctgga gcgagatttt ggatgtagcg gacagtcgca
     3961 ctcacattgg ttagagcggt tgtcaggatt acccccgctt ccttcgctaa gatcacgccc
     4021 tgtgtgccgg ctgtcgttcg caggatgacc cgtttggcag gatgaaccac agcattaatg
     4081 gtggaaggag agttgcccat atcaaatcct ggaacccgca ccccatccac ttctcccgct
     4141 atgatgcaat cgggcagttg gttgcgtaag gcgaaagctt ctttcacctg agaaacgaga
     4201 aagatctcct ccacgccagc gtcaaagagg taacctgccg ttgtaaaagc tcgcaacacg
     4261 tcaatgacga ccaccaaacc gctggctgtt ggacaggttg cgagcgttgc gcgctggatt
     4321 ttcatttcgt tgatcaggat gtttttatat ctctttcacc gtgatcttca gttccatggg
     4381 tgctactttc ttgaacattg cctgtgccga gcaatatttc gtttctgaaa gctccaccgc
     4441 gcgctccaca gcggcacgct caatgccttt cccgctcacc acatattcga tggtgagatg
     4501 ggtgaacact ttggggtgtt cctcagcgcg gtcagcgtgc acaagcacct cgaaatcacg
     4561 cacatcctgt tttttctttg ccaatatcga aatcacatcc atggctgtgc aaccggctat
     4621 gccgatcgcc aacagctcca gcgggcgaaa tccgtcgttg tttccaccca attcggcaac
     4681 cgttcccaat gggaccttga agccgctgtc agccgtacct tcaaagctca tgttctcttt
     4741 ccagatcact tttgcgtcca tttttctcct ttgtatgaac cccgccgggg ttggaacctt
     4801 tgtccagtga atccgtgccc aatctctaaa ccgcattggg ggcagtggat gttcgataaa
     4861 ccgttgtagt ccaagatgcc ctaaccgcag cgcgggcagc attttcctgc ctcccatcca
     4921 catagctgtt gagcggttcc tctttctttt ttctctgatc cctcagcctg ttcctgtttt
     4981 ttctcttctc tcattctttt gaaacggtaa ctaacgacca attgggtaaa acgtccatcg
     5041 caagatcatt tttaaatcca tgcgtgaagc gatggtaacc ggcgcttgcg atcatcaccg
     5101 cattatccgt acataatttt aacgggggaa tgtgaacggg aaactccttc tggtcgagga
     5161 atgccttgcg caaagcgcga ttggcagaaa ctccgcccgc caccaaaatg cttctggcgt
     5221 gaaattcgcg ggcagccgcc agagttttct ggcaaagcac gtctaccact gccgtttgaa
     5281 aactggctgc caagttctca accagcacag ggcgcccgtc ggcgcgcagt tcctgcaagg
     5341 tgtggagaac cgcggtcttc aatccgctga acgagaaatc ccagctcccc tccagccatg
     5401 cgcgcgggaa ttgaaatgcc tgaccgttcc cgctctcggc tgccttttga atagaaggtc
     5461 cacccgggta gggcaggttc agcaggcggg cgaccttgtc aaaagcctct ccagccgcgt
     5521 catccagggt gcgccctaat tgcgtatagg taagatggtc ctgcatcaat accagtgagg
     5581 aatgcccgcc cgaaacgatc aatgccatta ccggaaatgt cggttggctg tattcggggt
     5641 tatccagtgg tatccaggct gagtagaggt gcccttccag atggttgatc ccgatcaggg
     5701 gaatgccgct cgccaggctc aacccttttg ccatgttcat tcccaccacc agggaaccgg
     5761 ctaatccggg tccgcgcgtg actgcaatgg catccagttc gtggatgtcc agatgggcgt
     5821 ctttcaaagc ttgatcgacg accgcgtaga tggttttgat gtgctcgcgt gaggcaactt
     5881 caggaaatac cccgccgtat ctggcgtgca agtctgcctg cgatgccacg acatcgctca
     5941 aaacctgcga accatcttcc agaaccgctg ctgcggtttc atcacaagat gtttcaatgc
     6001 ccaggatacg aatgctcttt ttctcgttcg attgttcttc cataaattta ttattctttt
     6061 agcgggagga cgagctggta ggggaaatct gccagcatct ctaccttccc ctggtcattg
     6121 agatataccg tgtcttcgat ccgcacgcca aagcccttgg aagggtagta gagaccgggt
     6181 tcgatggtaa agaccgttcc gggctgcaaa atgtcacgcg caccggctcc cattccgctg
     6241 aaggggcttt catgcacgtc cagtcccaac ccgtgcccca ggctgtgcac atacccttct
     6301 gtggtttccg ggttctcttg gatggtctcg taaccgaact cacggaacat gcggcaggta
     6361 gccgcttggt aatcgcggaa gggagtgttc accttgagcg attccagcat gccctgatgg
     6421 acggtaaaga catcctgata gaccttttgg atttcctcag aggcataccc cagacaccag
     6481 gtgcgcgtga aatcgtagaa ataccctccc ccggcttcac atgggaagat atcgaaaacg
     6541 atggttttgc ctaactccat gcggtcgtct gcattgcctg cgctgtgcgg aattcccgcg
     6601 tcgcgtccga tcgagaagat cgtctcttca ggattgtcgg ctcccttttc tgccagccat
     6661 ctgcggatgt aagactttac cagcccaatc gttatcgggt tcccgtcttt gtcaaccagc
     6721 gtgtcatgct tagcccggca gttgcacaag aaatcggcag tgtttcccac cacttccgtg
     6781 gtgatctgac ccatcttgcg aatatgctcc acctcatccg cactttttgt aaggcgcgcc
     6841 aatttgatag gaccctcagt aaagcaaccg gcgaattctg tgcccggtaa taattcttgc
     6901 atagcgctga ccactgcgta aaaagcgctg agatcgtatc tgccggcgat ggcaatcttc
     6961 ttgttttgta tccccaggtc agtgaagata tctttgtagc gcagggcaat cgcctggaga
     7021 agatcaccgt tagtggtttg tgcatacttt cgcagagggt atttttcatc atatccttga
     7081 acggttaacc cggttttggc ggcttcttcc cgttccatgc tgttgtagaa aagaagcggt
     7141 tcctgatcac ggaccttgat cagatcggcg ctgctcacat gatggatccc tgtgaaatac
     7201 accatgtcgg gattattttg tgcggcaccg ctgatccata gcgcatcaat cccttgttct
     7261 tgcatgatcg catccaggtc ttttttcatt gccagctcct tgttattcct tccagtctga
     7321 taggattttt tgtagcgttc ccagaagttc ctgctcttga tgtgccagta ctgtgcgcgg
     7381 gtcaaggacc acgcagccct cttcgatgcg ggcgatgatc ggtgtttttt gttgacgtag
     7441 cactttcaga agctgatcag gtttctttac cttgagcgct aataaaaagg tgggcaagct
     7501 ctcttctggt aagctgccgc cgccgatcat ggaacgtcct tcaatgatat caccacaatc
     7561 cagcaaattc ttccactgaa gtgcacgctc agaaatggtc tgcgcgggca gtgccagcat
     7621 ttgccacacc ggaatttctt gttctgcctt tccggtcaga taagccagca gagtgctgct
     7681 gacccctgcc aggcaggctt tatcagggcg tagcgccctg gctagcgggt gtttcttgat
     7741 cttctcgatc aatccctttt tccctaagat gatgcctgct tgtggaccgc ccatcaattt
     7801 atcaccagaa aaactgacca catccacccc ggcttgcaaa gattcctgca ccatgggttc
     7861 atgaggcagc ccgtattttc ttgtgtccag cagcgctccc gaccccagat catggaacac
     7921 cagtaccccc ttttgatggg caagcgtgca gatacttgcc agcgacggtt cgctggtgaa
     7981 accgacgatc ttgaaattgg aatggtgggc gcataatacc agcgctgtcg gctcctgcag
     8041 ggcattttcg taatcgtaga gatgcaggcg attggtggtt ccgatctcta ccagttttgc
     8101 tcccgattgg cgcatcacgt ctggtacacg gaatcctccg ccgatctcca ccagttgtgt
     8161 gcgtgaaacc agaacacgtt tccgctgtgc cagtgcggtt agaaccaaca ataccgctgc
     8221 ggcattattg ttgacaatgg tcgccgcttc tgcgccgctg agccgggtca ggatccgctc
     8281 ggcgtgcacc gaacggctgc cgcgttttcc tgcatccaga tcatattcga gattattgaa
     8341 ggaaattgcg atattctctg tctcctggca ggtttttttt gaaaggggag cgcgccccag
     8401 gttggtgtgc aggatgaccc cgctggcatt gatgaccggc accagggtgg gtgcggtcca
     8461 tgcaagcaga agatcttcac tttcgtcgat gatcttctct tcggtgaatg ctttcccacc
     8521 tttgcgtatt ccatccaggg cttgtcgcag ggcttccacc accagttcat gcccgtaggt
     8581 atccaataat ggcagcgtac tttttaaatg gagaagtcta tcaatagaag gaaaatggct
     8641 gcggtcagtc atcttttttt cctcctgcaa aaaaaactgc tctttcactg atccgcaaga
     8701 agatttccag cagcacaaat cccattccca gcagtagcat gatgaggttc gaaagaaccc
     8761 tgccaagctg tagccagagt aaaaggtcaa cgtatgctga gaaaagaatc gtttcgcgca
     8821 gcagcacccc ttcggtcaga ttctttttgg aaaaacgaag ttgaatcatg gtgatgatcg
     8881 gaaggctcac cccacatgcc agtagcgata caaaaaagaa gagcaaccag cgtggaccga
     8941 gaaaaggttg catgttgaag agcaccagca acaatccggc tccccctgcg gcagtcagga
     9001 ttaaccccac cggcagcacc tttccgagtg tgatctcttg ctttttcatc atatgaaaag
     9061 attatatcgt aagccaattt ccatggaaaa gaaatgcgcg gtatacttac tttcctgatc
     9121 aacactttgg agggcgcgtt gctcaagaaa atcacttcaa cctttaaaga atacccacag
     9181 agttttcaga tcctggcagg agctaccttt atcgatcggg tagggggcac catgatccag
     9241 cccttctatt cattgtatat cgcgcaaaaa ttcggcgtca atatgaccac catcggattg
     9301 ctgttcggta tctgggctgt ctcaggcgtg gtggggaata tgctgggcgg agccctgacc
     9361 gacaagatcg ggcgcaagac tatgctcgtg gcagggctgg tgctttcagc taccaccgcg
     9421 atcttgatgg gtatcgtgaa taatcttgtg gcgtttgccc tcatctccac cttggtgggt
     9481 atgctttcgg atattgccgg tccggcgcgc ggcgccatga ccaccgatat gctaccggtt
     9541 gacaagcggg cagaaggttt tggcatcatt cgtgtggtag gaaacctggc gtggatcatc
     9601 ggtccggctc tgggtggttt gctggctagc cggtcgtaca tgatggtttt catttgcgat
     9661 gccattgcca gcatcatcac cgcgttgatc gtggtatgga aaattccgga gaccaagccg
     9721 caggcaggcg aagaaaccga aaaagagacg ttgctgcaaa ccttcaaagg atacgggcag
     9781 gttgtctccg ataagctgtt catggtatat gtgcttatct ccatgctatt gatttttgtg
     9841 tacggtcaga tgtacagcac gctgtctgtt tatctcaacc agcagcatga tattcccgcc
     9901 aaggggtacg gttggttgat gagcatgaat gccctcatgg tggttctttt ccagttcagc
     9961 gttacccgcg gcacgcgcaa aaaaccgcct atgtatatga tggcattagc taccc
//
LOCUS       DAOSDS010000078         9832 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2599_length_9832_cov_3.952235, whole genome shotgun sequence.
ACCESSION   DAOSDS010000078 DAOSDS010000000
VERSION     DAOSDS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2599_length_9832_cov_3.952235"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /gene="folE"
                     /locus_tag="PLL88_08725"
     CDS             <1..363
                     /gene="folE"
                     /locus_tag="PLL88_08725"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOJ01688.1"
                     /translation="YSLCEHHMLPFIGRAHVAYLPQEKVIGLSKIPRIVDMFAHRLQV
                     QERMTRQIADFMNILLKPRGVAVVMEAVHMCAMIRGVKKHEARMTTSSMLGAFRNREA
                     TRMEFLDNISRGARPLEF"
     gene            complement(360..830)
                     /gene="folK"
                     /locus_tag="PLL88_08730"
     CDS             complement(360..830)
                     /gene="folK"
                     /locus_tag="PLL88_08730"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOJ01689.1"
                     /translation="MHHLHLLVGSNIEAQQNIKAAMKMLESVHPIKACSRIWKTIAYG
                     SQGPDFLNIALCVETDFSVEEYKKNVIVPIEIALKRQRFPDKNAPRTIDLDIIVYDGE
                     VIDTGLWDKFFITVPIADLLPELKDPVSGLPLRRIAQSVIEQGLATFYANCLEP"
     gene            complement(831..1190)
                     /gene="folB"
                     /locus_tag="PLL88_08735"
     CDS             complement(831..1190)
                     /gene="folB"
                     /locus_tag="PLL88_08735"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOJ01690.1"
                     /translation="MDKVFINDLMVRGVIGISDRERSQAQDIVVNVVLYTDITKGGET
                     DNIDDCVNYRTIAKAIIAHVEKVSRYTVEALATDIAALCLNTEGVQKVRVKVEKPGAV
                     RFSKSVGVEITRKKESA"
     gene            complement(1197..1922)
                     /locus_tag="PLL88_08740"
     CDS             complement(1197..1922)
                     /locus_tag="PLL88_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ01691.1"
                     /translation="MSTLKDKQVLITGASKRIGREFALAAAREGATIFLHYNSSESDA
                     QKTKQAVIESGGACQLLQADFRDPHQAVPVFEQLFAAHEIYALVNNASIFLQNTLRNT
                     TLPDWELHQNINLTMPFALTQAFANSPRIASGRVINVLDWRALRPGKDHFAYTISKAG
                     LAALTKASALALAPSFQVNGLALGAILPPSDGSDTSTIIQQVPAGRWANLDELTATFL
                     FLLTAPQYITGEIIHVDGGRHLV"
     gene            2047..2250
                     /locus_tag="PLL88_08745"
     CDS             2047..2250
                     /locus_tag="PLL88_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOJ01692.1"
                     /translation="MNKTYYLPNIHCQHCVHTVKMELEEIDGVEKVDVDLNTKKANVS
                     MKSAEVEQSVLKTLDEIGYPAEL"
     gene            2268..4691
                     /locus_tag="PLL88_08750"
     CDS             2268..4691
                     /locus_tag="PLL88_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOJ01693.1"
                     /translation="MENTKNIILPIQGMTCANCVATIERNLKKEKGVEAVSVNLSSER
                     AVVNFSENETNLAALIQRVERAGYGVLQVENDILLEKLSDAHEVDRIVEKLSALEGVM
                     KVTGNVVKETITISYIPTMVDLREIYNALRKAGFRPIIPADSLDSEQKAREREIRHQK
                     KLLIVGLIFTIPLFVLSMAHDFGLLPRFLHMADWLDYLFLALATPVQFYVGAQFYAGA
                     VKSLRGGSANMDVLIALGSSVAYIFSILVMAGVVQGHLYFETSAVIITLIRLGKFLEA
                     KAKGRTSEAMKKLVNLQVKSATVKRNGVEVKVPAEEIQLGDLIVVRPGEKVAVDGEVV
                     EGHTSIDESLITGESLPVNKQIGDEVIGGTINKQGVITFKATKVGKETVLSQIIKMVE
                     EAQSSKAPIQRLADTVSAYFVPIVILIALFTFVLWYFILKIPVSEADQTVFSRAIINM
                     TAVLVIACPCAMGLATPTAVMVGSGKGAERGILFRSAEALERAGKVDTLVFDKTGTIT
                     KGQPSVIDIHVFDSEYTENDLLKLAASVENGSEHVLGEAILAEAGNRGVQPLEMSEFQ
                     AFAGMGVAAKVDGRMVELGNERFLQQQGVAYMPDQKVSDILEQKALTPIYIAVNKHLA
                     GVFGIADQIKNESIATVEKLKNMGMKVVLLTGDNAATAKAIARQAGIEDVLADVLPMG
                     KADAINELQKQGRAVAMVGDGVNDAPALAQADVGIAVGTGTDVALSTAPITLLSGELG
                     KVVEVIRLSKKTLKTIKQNLFWAFFYNVILIPVAAMGYLSPMFAAGAMAFSSVFVVTN
                     SLRLKKAKI"
     gene            4812..6818
                     /gene="tkt"
                     /locus_tag="PLL88_08755"
     CDS             4812..6818
                     /gene="tkt"
                     /locus_tag="PLL88_08755"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOJ01694.1"
                     /translation="MSIKAELEMKAIKTLRYLSVDGVQTANSGHPGLPMGTAAIAYTI
                     WTRHLRINPNNPGWFNRDRFILSGGHGSMLIYSLLHLTGFDLPLDELKNFRQWGSRTP
                     GHPEYGLTPGVEVTTGPLGQGFANGVGMAIAEAHMAAEFNEPEHAIVDHYVYAIVTDG
                     DLMEGVASEAASLAGTLKLGKLIYLYDDNRISIDGSTDLAFTEDRALRFEAYGWYVQK
                     VEDGNDVEALDAAIRKAKQDARPSLIVCRTHIGYSLPTKQDTAAVHGTPPGWSEIDAS
                     KEAQGWPVEPHFYIPEDVLEHYREVKQNGMAYENEWQALFAEYQKKYPHKAAELKRRI
                     EGKLPENWGEDLPEFPADAKGMATRISSGKVINALVEKLPEMVGGSADLTPSTKTWID
                     QSSAFSAENRLGANIHFGVREHGMGAILNGMAVHKGVIPFGATFLVFSDYNRPSIRVS
                     AISHYPVVWVFTHDSIGLGEDGPTHQPIEHLASLRAIPNLVTLRPADANETREAWKIA
                     VERKNGPTALILSRQDVPTLDREKLAAASGLRQGAYVLADYGGGTPEIILMASGSEVQ
                     LVLGAAMALADKGKRVRVVSFPSWELFADQSKEYQDAVLIPSVKNRLAVEAGVKQGWE
                     KWLGEKGVMIGMTGYGASAPAEVLYKKFALTIENIVMHANQMLD"
     gene            6830..7288
                     /gene="rpiB"
                     /locus_tag="PLL88_08760"
     CDS             6830..7288
                     /gene="rpiB"
                     /locus_tag="PLL88_08760"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOJ01695.1"
                     /translation="MKVAIGSDHAGFPLKTEVIDVVRNAGYEVLDVGTTSLEAVDYPD
                     FAYKVGKAVQEGRAERGITICGSGIGACIASNKMKGVYAGICHDTYSAHQGVEHDGMN
                     VLCMGSRIIGSELAREIVLSFLKASVEEAARHQQRRRKIREIEQGTYTGE"
     gene            7288..>9832
                     /locus_tag="PLL88_08765"
     CDS             7288..>9832
                     /locus_tag="PLL88_08765"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HOJ01696.1"
                     /translation="MEHSDELKKIGQSLWYDNIQRDLLKDGTIQRLIDSEKITGITSN
                     PSIFKKAILSSARYQFPLKIMAWAGWQAGEIYEQLAVEDISEAADLLLPCYRATNGRD
                     GYVSLEVDPELAHDTQATIKDAQRLWKRVARPNLMIKIPATKAGIPAIRSAIAAGINV
                     NVTLIFSIQRYQEVIDAYMQGLEDRVGQGLPIHGIASVASFFVSRFDTKIDAMLEKLP
                     DSEAKALYGKAAVANARMAYRTYQTAFASERFLALKEKGVQVQRPLWASTSTKNPAYR
                     DVLYIEELIGPDTVNTVPPATLDAFFAHGEVQDKLSAPSVEDDRVVEKLSGLGVDLEQ
                     VARELEEEGVQQFQDAFREMLQALDSERNTYSANLGSLKDAVRNRMHTFEETGFPAAL
                     FQKQAQLWTRSPEGQEEVRNRLGWLEAPRLGISQLSHIHNIARELGKDGFKRVVLVGM
                     GGSSLAAEVMAFILKTQEGGLALRILDSTDPMQVAHMMEWAEEEKTVFLISSKSGSTA
                     EINACFQYAWERVKTAGADNPGERFIAITDPGTTLQDLAEEHHFRAVFLADPTVGGRY
                     SALTAFGLVPAGLLGVPLEPLLKTAQAMREMCDPAVATGRNPGLVLGAILGEAYLHGK
                     DKLILRTDQEWLSFGSWVEQLIAESSGKEGKGILPIDQEPALPADKYASDRLFVYLRS
                     DGSQDEHIRELAAAGHPSLVIPVDGEYMLAEQFYLWEFATAVACAILGVNAFDQPNVQ
                     DSKTRTKQKIKEIEETGALVQGKPVWEDAELQLFSHEKFDLRAGDLAAIIDQFLEKAT
                     ATDYVAINAFVERNEKHQSILQSLRKHIAEKYQIATTLGYGPRFLHSTGQ"
BASE COUNT         2525 a         2273 c         2713 g         2321 t
ORIGIN      
        1 tacagtttgt gcgagcacca tatgctgccg tttatcggca gggcacacgt ggcatacctg
       61 ccccaggaaa aagtgatcgg actttcaaag atcccgcgca ttgtggatat gtttgcacat
      121 cgcctgcagg tgcaggaacg catgacgcgc cagatcgcag attttatgaa cattcttctt
      181 aaaccacgcg gagtggctgt ggtgatggaa gcggtgcata tgtgtgccat gatccgcggt
      241 gtgaagaagc acgaggcgcg catgaccacc tcttctatgc tgggcgcttt ccgcaaccgc
      301 gaagccacca gaatggaatt cctcgacaac atttctcgcg gagccagacc actggaattc
      361 taaggttcca aacagttcgc gtagaaagta gccagaccct gctcgattac gctctgcgcg
      421 atgcgccgca gagggagacc ggagacaggg tcttttaatt caggcaagag atcggctatg
      481 ggaacggtga tgaagaattt gtcccaaagt cccgtatcaa tcacttcgcc gtcatataca
      541 atgatatcca gatcgatcgt acggggtgcg ttcttgtcgg gaaaacgctg gcgtttgaga
      601 gcaatctcga tcggaacaat gacgttcttt ttatactctt caacgctgaa atctgtttcg
      661 acgcagagcg caatatttaa aaagtcagga ccctggctgc cataggcaat ggttttccag
      721 atgcgcgaac atgctttaat gggatgcacg ctttcaagca ttttcatagc ggctttgatg
      781 ttttgttggg cttcaatgtt ggatccaacc agcagatgaa gatgatgcat tcaagcactt
      841 tctttttttc tggtgatctc cacccctacc gatttggaga agcgcacggc acccggtttt
      901 tcgaccttca cgcgcacctt ttgcacgccc tcggtattca agcatagcgc cgcgatgtct
      961 gttgccagcg cttctaccgt atagcgggag accttttcga catgcgcaat aatcgctttc
     1021 gcgatggtgc ggtaattgac gcagtcgtca atgttatccg tctcgccgcc tttggtgata
     1081 tcggtatata acacgacatt gacaacaata tcctgcgctt gagagcgctc gcggtcgcta
     1141 atgccgatca ccccacgaac catcaggtcg ttaataaata ctttatccat aagtgcctat
     1201 accaagtgcc ttcctccatc aacgtggatg atttcgccgg tgatgtattg tggcgcagtc
     1261 aataagaaca ggaacgttgc ggtcaattca tccaggtttg cccaacgacc ggcgggaacc
     1321 tgttggatga tagtgctggt atcgctgcca tcagaagggg gaaggatggc gcccagcgcc
     1381 agaccgttca cctgaaaaga cggggcgaga gcgagggcag acgctttggt caatgcagcc
     1441 aaaccagctt tgctgatggt gtaggcaaag tgatctttgc ccggtcgcag ggctcgccaa
     1501 tccagaacgt tgataaccct gccggaagcg atacgagggg aatttgcaaa tgcttgtgtc
     1561 aatgcaaaag gcatggtcaa attgatattc tggtggagtt cccaatctgg cagggttgta
     1621 ttgcgcaggg tattctgcag aaaaatggaa gcgttgttca ccagcgcgta tatctcgtgc
     1681 gcggcgaaaa gctgttcaaa aacaggaaca gcctggtgcg ggtcacggaa atcagcctgg
     1741 agcaactggc aagctccccc gctctcgatg acagcctgtt ttgtcttttg cgcatcgctt
     1801 tcagagctgt tatagtgcaa aaaaatggtt gccccttcgc gcgccgctgc cagggcaaat
     1861 tcgcgcccaa tgcgtttcga tgcgccagtt atcaagacct gcttatcttt gagtgtgctc
     1921 attcatcaac ccttattctt atcctattga aaaactctga ttcgtcaagt gtaagaattc
     1981 tgcaaaaata ttaataatta tgaaaaaaat ctataatata ataggtatca aaagaaagga
     2041 cgacgaatga acaaaactta ctatttgccg aacattcact gccagcattg tgtacatacc
     2101 gtgaaaatgg aactggaaga gatagacggc gtggaaaaag tggacgtcga cttgaacacc
     2161 aagaaagcaa acgtatccat gaaatctgca gaggttgaac aatctgtatt aaaaacattg
     2221 gatgaaatcg gctacccggc tgaattgtaa attgttgaga aaaagacatg gaaaacacga
     2281 aaaatatcat tttaccgatc caggggatga cctgcgccaa ttgtgtggca accattgaac
     2341 gcaacctgaa gaaagaaaaa ggggtggaag ccgtttccgt gaacctgtct tcggagcggg
     2401 cagtggtgaa tttttctgaa aatgaaacca atcttgctgc tctcatccag cgtgtagaac
     2461 gagctggtta tggcgtgctg caggtcgaaa atgatatctt gcttgagaaa ttaagcgatg
     2521 cgcatgaggt ggatcgcatt gttgaaaagt tgagcgcatt ggaaggggta atgaaggtga
     2581 ccggaaatgt ggtgaaagaa acgatcacca tttcttatat ccctactatg gtggatttac
     2641 gagagattta caatgcgctg cgcaaagcag ggtttcgccc cattattccg gcagatagcc
     2701 tggattcaga gcagaaggcg agggaaaggg aaattcgcca tcagaaaaaa ttgctgatcg
     2761 tcggattgat ctttaccatt cccctgtttg tgctttctat ggcgcacgat tttggattgc
     2821 tgccgagatt tctgcacatg gcagattggc tcgactacct tttcctggct ttggctaccc
     2881 cggtgcagtt ctatgtaggc gcacaattct atgcgggggc tgtgaagtcg ctgcgcggtg
     2941 gttctgccaa tatggacgtg ttgatcgccc tcggctcgtc ggtggcatat atcttttcga
     3001 tcctggtgat ggcaggggtg gtgcagggac acctgtactt tgaaacctcg gcggtgatca
     3061 ttaccctgat ccgtctgggc aaattccttg aagcgaaagc caaggggcgg accagcgagg
     3121 cgatgaaaaa gttggtcaac ctgcaggtga agagcgctac cgtcaagcgc aacggtgtgg
     3181 aagtgaaagt ccctgctgaa gagatccaac tgggagatct cattgtcgta agaccgggcg
     3241 agaaggtggc tgttgatgga gaggttgtgg aaggtcacac gtccatcgat gaatcgctga
     3301 tcaccggaga atccttgccg gtgaataagc aaatcggaga tgaggtgatt ggcgggacga
     3361 tcaacaagca aggcgtgatc acgttcaaag ccacgaaagt aggaaaagaa accgttctct
     3421 cacagatcat taaaatggtg gaagaagctc aaagcagcaa agctcccatc caacgcctgg
     3481 cggatacggt atcggcttat tttgtcccca ttgtcatcct catcgcattg ttcacctttg
     3541 ttctgtggta cttcatttta aagattccag tttctgaggc agatcagacg gtcttttcgc
     3601 gcgccatcat caatatgacc gcagtgctgg tcatcgcctg cccctgcgct atggggttgg
     3661 caacgcccac cgcggtgatg gtaggatcgg gcaagggagc tgaacggggg atcctgttcc
     3721 ggtctgccga ggcattggaa cgtgccggaa aagtggacac actggtattt gataaaaccg
     3781 gcacgatcac caaagggcag ccttctgtga tcgatattca cgttttcgac tcagaataca
     3841 cagaaaatga tcttttgaaa ctggctgcaa gcgtggaaaa cggaagcgag catgttctag
     3901 gcgaagccat tttggcagaa gccgggaacc ggggcgttca accgctggaa atgagcgagt
     3961 tccaggcgtt cgccggtatg ggagtggctg ccaaagtgga tggtcgcatg gtggaactgg
     4021 gaaatgaacg attcctacag caacagggtg tcgcttacat gccggatcag aaggtttctg
     4081 atattcttga acagaaagct ctcacgccga tctatatcgc cgtgaacaag catctagcag
     4141 gcgtttttgg gatcgcagac cagatcaaga acgaatctat cgcgacggtc gaaaaactaa
     4201 aaaatatggg catgaaggtg gtccttctca ccggagataa cgctgcaact gccaaagcca
     4261 ttgcacgcca ggcgggcatc gaagatgttc tggcggatgt gctgccgatg gggaaggcag
     4321 atgcgatcaa cgaactgcag aaacaaggaa gagccgttgc catggtgggg gatggcgtga
     4381 atgacgcccc cgcgctggca caagccgatg tgggaattgc tgttggaaca ggaacggatg
     4441 tagcgctttc aaccgcaccg atcacgttgc tgagcggtga gttggggaag gtggtagaag
     4501 ttatccgtct atccaaaaag accttgaaaa cgatcaagca gaatctcttt tgggctttct
     4561 tctacaacgt tatcctgatc cctgtagcag ccatgggcta tctcagcccc atgtttgccg
     4621 ccggagctat ggcgttctcc agtgtatttg tggttaccaa tagcttacga ttgaaaaaag
     4681 cgaaaattta atctgcgcat tgcattgcgc tttttctgaa aaagcaattt gggtcttcag
     4741 ggggaattgt tttcatcctt aactttaacc gcatggtaga ataacaaaaa tcaaatccca
     4801 aggaaataat catgagtata aaagctgagc tggaaatgaa agccatcaag actttacgat
     4861 atttatcagt agatggcgtg cagactgcca attccgggca tcccggcttg cccatgggca
     4921 cggctgccat agcatatacc atttggacgc gtcatctgcg catcaatcca aacaatccgg
     4981 gctggttcaa ccgcgaccgt ttcatcctct cagggggaca cggatcgatg cttatttaca
     5041 gtttattgca cttaacgggt tttgaccttc cgttggatga gctaaagaat ttcagacagt
     5101 ggggcagccg cactcccggt catccggagt atgggttgac acccggcgtg gaagtcacca
     5161 ccggtccatt gggacagggc tttgccaacg gggtggggat ggcaattgcc gaagcgcata
     5221 tggcggctga attcaatgaa ccagaacatg cgattgttga tcattacgtt tatgctattg
     5281 ttacggatgg cgaccttatg gagggcgttg cttcggaagc ggcttctctg gctggtactt
     5341 taaaactggg aaaattgatc tatctatatg atgacaaccg catttccatc gatggttcga
     5401 cggacctggc atttaccgaa gatcgcgccc tgcgctttga ggcatatggc tggtatgtgc
     5461 agaaggtgga agatgggaat gacgttgaag cgctcgatgc agcgatccgg aaagccaagc
     5521 aggatgcgcg cccttcgttg atcgtatgcc gcacccatat tgggtacagc ctgcccacaa
     5581 aacaggacac agcagcggtc cacggcacac cgcccggttg gagtgagatc gacgcatcca
     5641 aagaagcaca aggatggccc gtggaacctc atttttatat ccctgaggat gtgctggagc
     5701 attaccgcga ggtcaagcaa aatggcatgg cttatgagaa cgaatggcaa gccctgtttg
     5761 cggaatatca gaaaaaatac ccacacaaag cggctgagtt gaagcgacgg atcgaaggaa
     5821 agttgcccga aaattggggg gaagacttgc ctgaattccc ggctgatgca aaagggatgg
     5881 caactcgcat ctcttcagga aaggtcatca acgcactagt cgagaaattg cctgagatgg
     5941 tgggtggatc tgcagatctg acaccctcta ccaaaacctg gatcgaccaa agcagcgcat
     6001 tttccgccga gaatcggctg ggcgccaata ttcacttcgg ggtgcgcgag cacggcatgg
     6061 gtgccattct gaatggaatg gcagtgcata aaggcgtcat cccatttggc gcaacttttt
     6121 tggtgttctc tgattacaat cgtccctcca tccgcgtttc cgcgatctcc cactatccgg
     6181 tggtgtgggt attcacgcat gacagcattg ggctcggtga ggatggaccc actcatcaac
     6241 cgattgaaca cctggcatcc ttgcgtgcca ttccgaacct tgtcactctt cgtccggcgg
     6301 atgccaatga aacacgcgaa gcctggaaga ttgctgttga acgcaagaat ggacctaccg
     6361 cattgatcct cagccggcag gatgttccca cgcttgaccg ggaaaaactc gctgcggcaa
     6421 gcggattgcg gcagggggcg tatgtgttgg ctgattatgg aggaggcacg cccgagatca
     6481 tccttatggc gagcggttcc gaagtgcagc tcgttttggg cgctgccatg gctctggctg
     6541 ataaaggcaa gcgggttcgt gtagtttcgt ttccctcgtg ggaattattc gcagatcaat
     6601 ccaaagaata tcaggatgcg gtgctcatcc catctgtgaa gaaccgcctg gcggtagaag
     6661 caggcgtgaa gcagggttgg gagaaatggt tgggagaaaa aggcgtgatg atcggaatga
     6721 ccggttatgg cgcatctgct ccggcagaag tcttgtataa gaaatttgca ttgacgatag
     6781 aaaatatcgt aatgcatgcc aaccaaatgc tggattgaaa ggaatcgtca tgaaagttgc
     6841 gatcggatct gaccatgcag gttttccatt gaagaccgaa gtgattgacg ttgttcgaaa
     6901 tgcgggttat gaagtgttgg atgtcggcac aacttcgctc gaggcagtgg attatccgga
     6961 ttttgcttat aaggttggaa aagccgtcca ggaagggagg gcggagcggg gcatcacaat
     7021 ctgcggctcc ggaataggag cctgcattgc atctaataaa atgaaggggg tctacgccgg
     7081 catatgtcat gatacctatt ctgctcacca gggtgtggag catgatggta tgaacgttct
     7141 gtgcatgggg tctcgcatca tcggttcgga attggcgcgt gaaatcgttt tgagttttct
     7201 caaagcatcc gtagaagaag cggcacgcca ccaacaacgt cgccggaaaa tacgggagat
     7261 agaacaaggt acatatacag gtgaataatg gaacacagcg atgaactgaa aaagatcggt
     7321 caatccctat ggtatgacaa catccagcgt gatctattga aggatggaac tatccaacgg
     7381 ttaatcgatt ctgagaagat cacaggtatc acatccaacc catccatatt caagaaagcc
     7441 attctcagtt cagcgcgcta tcaatttcca ttaaagatca tggcatgggc tggttggcag
     7501 gcgggggaga tctatgagca attggcagtg gaagatatca gcgaagctgc cgatttgttg
     7561 ttgccatgtt atcgcgccac aaatggcagg gatggttatg tgagtctgga ggttgacccg
     7621 gaactggcac atgacacaca ggcaaccatc aaagatgctc aacgtttgtg gaagcgggtt
     7681 gctagaccca acctgatgat caagataccc gccacaaaag ctggcattcc agccatccgc
     7741 agcgccatcg cagcaggcat caatgtgaac gttaccctga tcttttcgat ccagcgttat
     7801 caagaggtga tcgatgcgta tatgcaggga ttggaagacc gggtggggca ggggttgccc
     7861 attcatggga ttgcttcggt agcatccttt tttgtatccc gttttgatac caagattgat
     7921 gcgatgttgg aaaagctccc tgactccgaa gcaaaagcct tgtacggaaa agccgcagtt
     7981 gccaatgccc gcatggcgta ccgcacctat caaactgcct ttgccagcga gcgcttcctg
     8041 gctttaaaag agaaaggcgt tcaagtacaa cgaccactat gggcttccac cagcaccaag
     8101 aaccccgctt atcgcgacgt gttgtatata gaagaattga tcggacccga tacagtgaac
     8161 acggttcctc cagccactct cgacgctttt tttgcccatg gcgaggtaca agacaaacta
     8221 tctgctcctt ctgttgagga tgatcgggtt gttgagaaat tatccggact gggcgttgac
     8281 ctggagcaag tggctcggga gttagaggaa gaaggtgttc agcagttcca ggatgcgttc
     8341 cgggaaatgc tgcaagcttt agatagtgag cgcaacactt attctgccaa cctgggttct
     8401 ttgaaggatg ccgtgaggaa ccgcatgcac acgtttgaag aaacaggctt tccggcagcg
     8461 ctatttcaaa agcaagcgca attgtggacc cggtctcctg aagggcagga agaggtgagg
     8521 aaccggctgg ggtggcttga agctccccga ttgggaatct cacagctatc ccatatccac
     8581 aacattgcac gagagttggg aaaagatggt ttcaaacgtg ttgtcttggt cggcatggga
     8641 ggttcatcgc tagccgcaga ggtcatggct ttcattttga agacccaaga gggagggctt
     8701 gctctgcgca ttctggattc aaccgacccc atgcaggtag cccatatgat ggaatgggct
     8761 gaagaggaga aaacggtttt cctgattagc agcaaatcag gatccacggc tgagatcaac
     8821 gcttgctttc aatatgcctg ggaacgggta aagacggctg gagcggataa cccgggtgag
     8881 cggttcatcg cgataaccga ccccggcacc actttgcaag atttggcgga ggaacaccat
     8941 ttcagggctg tctttctggc agatcccact gtgggtggaa ggtattcggc gctcactgct
     9001 tttggtctgg ttccggcggg attgctaggg gttcctttgg aaccactctt gaagacagct
     9061 caggcaatgc gagagatgtg tgacccggct gtcgcaacgg gcaggaaccc tggtttggtc
     9121 ctgggtgcga ttctgggcga ggcatatttg cacgggaaag acaaactcat tttacgcacc
     9181 gaccaggagt ggttgtcttt tggatcctgg gtggaacaat tgattgcgga aagcagcgga
     9241 aaagagggca aaggcattct gccaatcgat caggagccag cattacccgc tgataaatat
     9301 gcgtcggatc gcctttttgt gtatttgcgt tctgatggga gtcaggatga acacatccga
     9361 gaacttgcag ccgcagggca tcccagcctg gtgatcccgg tggacggcga gtatatgttg
     9421 gctgagcaat tctatttatg ggaattcgcc actgcggttg cctgcgcgat tttgggagtg
     9481 aacgcttttg accaacccaa tgtgcaagac agcaagactc gcaccaaaca aaagataaag
     9541 gagatcgaag aaacaggcgc cctggtgcaa gggaagcctg tatgggaaga tgcagagctt
     9601 caattattct cccacgagaa attcgatctt cgggcaggcg atctggcagc tatcattgac
     9661 cagtttctgg aaaaagcaac tgcgaccgat tatgtggcga tcaatgcttt tgtggaacga
     9721 aatgaaaagc accagtccat ccttcaatcc ctgcgaaagc acatcgctga gaaataccag
     9781 attgccacaa cattgggata cggtccgagg tttctgcatt caaccgggca ac
//
LOCUS       DAOSDS010000079         9678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2647_length_9678_cov_5.276837, whole genome shotgun sequence.
ACCESSION   DAOSDS010000079 DAOSDS010000000
VERSION     DAOSDS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2647_length_9678_cov_5.276837"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="PLL88_08770"
     CDS             <1..900
                     /locus_tag="PLL88_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HOJ01697.1"
                     /translation="VDMWITINEPDVYSFSGYMTGEFPPGKSDPQAAFRVLANLVRGH
                     AMAYHAIHALQPRALVGFAKNYRSIVPARTWFPPDHWIRNFSHNAFNNAFANTLLDGK
                     LRFILWKESIPQAIGTQDYIGVNYYTQDNIKFNVLSAKSFFQERFFTPNAELSENGFI
                     ATEPDGFAESLKWARRYRVPIYITENGVEDSHDAMRPHYLVSHVLEMWKAIQNGMDIR
                     GYMHWSQIDNFEWERAWTQRFGLWGLDVETQQRIHRPSVDIYRAICEQNAIPVENVLR
                     YTSELKALEEWYDTGYGNFSKKN"
     gene            complement(1023..1199)
                     /locus_tag="PLL88_08775"
     CDS             complement(1023..1199)
                     /locus_tag="PLL88_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01698.1"
                     /translation="MYSFIPAILFFGVGVILYRRESKKEPDTRNKANIMLASYACIVG
                     IALAVVSLLYWGEL"
     gene            complement(1268..2110)
                     /gene="mutM"
                     /locus_tag="PLL88_08780"
     CDS             complement(1268..2110)
                     /gene="mutM"
                     /locus_tag="PLL88_08780"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOJ01699.1"
                     /translation="MPELPEVETIARSLSQGRGAGFSILGHMIRDVSILWERSIAVPS
                     AQEFSQRICGQTIRETGRRGKYLVVRLSTDTLLFHLRMSGDLYVRPAAVQDGNQIPAK
                     HDRVILWLENEKSLVFEDARKFGRVWLLTDPEEELGKLGPEPLSENFKAEEFYRMLQG
                     SKRQLKPLLMDQTFLAGLGNIYTDEALHRAKLHPLSTASALSGEQAATLLRAIQETLQ
                     EGIRRNGASIDWIYRGGDFQNQFRVYGRKGEACATCGASIERIVVGQRGTHFCPSCQK
                     KIAG"
     gene            complement(2110..2847)
                     /locus_tag="PLL88_08785"
     CDS             complement(2110..2847)
                     /locus_tag="PLL88_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HOJ01700.1"
                     /translation="MQKVEISEINNYVNENIQCFHTTRLECISSLKLSKLLRKNPYLF
                     KAKNINVASDLIGSLLDAFLSSSEEKIFGDFLEGLAVFITSKTSNGHKSSANGIDLEI
                     LRDNKHYLVSIKSGTNWGNSSQQKRQIEDFNKASAILRQLDKSLNVQAILGICYGKTR
                     TSYLHNYWKLVGQNFWFFISGMESLYLNIIEPIGYKAKEHNEKSKITNRFTKEFIEKF
                     CNNNYEIDWNKLVNFNSGNLDIKIDEI"
     gene            complement(2861..3715)
                     /locus_tag="PLL88_08790"
     CDS             complement(2861..3715)
                     /locus_tag="PLL88_08790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOJ01701.1"
                     /translation="MEYVNKIILGDCGEVLKNLPDNCVDLIFTSPPYADQRKNTYGGI
                     HPDKYVEWFLPKTLEFKRVLKPTGSFVLNIKERVVKGERHTYVLELILALKKQGWLWT
                     EEYIWHKKNSFPGKWPNRFRDSWERLLQFTKGKDFKIFQDTVMVPIGNWAKDRLSNLS
                     ETDLTRDESKVNSGFGKNISNWVGRKSVYPTNVLYLATECSNKNHSAVFPVELPKWFI
                     KLLTEPGDLVLDPFVGSGTTCLAAMELGRNYVGIDVVENYIELSKERLSGVQIRIPSV
                     AEEQSNYK"
     gene            complement(3757..4749)
                     /locus_tag="PLL88_08795"
     CDS             complement(3757..4749)
                     /locus_tag="PLL88_08795"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOJ01702.1"
                     /translation="MTTSSLRHDRMMYGGVKLYAGSACPELAESIASYLEISLCKTTI
                     TTFPNENLFVKLHTSVRGQDCYVIQTLSSPVHTNLMEMLILIQTLRLDSAGRITAVFP
                     YMCYSRSDKKDQPRVPITARLVADMIQAAGADRYMTLDLHADQIQGFFSIPGDVLYAS
                     HVLIDYLLEIMKKMKSPVLLSPDLGFAKKARNYAEQLNIPLAFIEKRRILSPDNPEVL
                     TLIGDVKGKDVILLDEEVSTGKTIINAVNVATKNGAKDVVVVFIHPILAGDAVKQMAK
                     LPVKQFITTDTIPISKEKQAQFGGRLRIISVANLIGEVIRRVNEGRSVGEMFNE"
     gene            complement(4752..5291)
                     /locus_tag="PLL88_08800"
     CDS             complement(4752..5291)
                     /locus_tag="PLL88_08800"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOJ01703.1"
                     /translation="MIIGDTVRLRGIDKEDLPLFVSWLNDADVRRYLSLYSPLSNAQE
                     EQWFADLAKRPLTEQPLMIETVEDGTWKPLGNISFLNVDQQSRNAELGLFIGEKALWG
                     KGIGAKAIALMLDYGFKTLNFHRIYLRVFAPNQRGIHCYEKLGFRHEGSMREAYFLDG
                     EYIDILYMGILEDEWRGKK"
     gene            complement(5302..6624)
                     /gene="aspS"
                     /locus_tag="PLL88_08805"
     CDS             complement(5302..6624)
                     /gene="aspS"
                     /locus_tag="PLL88_08805"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HOJ01704.1"
                     /translation="MQRISIGDLSNHIGQEVKVQGWLQILRDQKKMQFLILRDRTGLV
                     QVAHYRPSNEELANQISTLGVESALTITGKVVKNDVVKLGGLEIQLQSLTVENAAEIP
                     MPYDPFDDANTPAVDFRMDWRYLDLRRPQNLLIFEVQTTLEHAMREFWLQHDFVEIHS
                     PKIMGSPSESGAELFQLEYFERKAYLAQSPQFYKQMGMAAGFERVFEVGPVFRADPSF
                     TSRHMTEFTGLDVEISWIDSHEDVMAFTERWLQYAYQQVKEKNGEQIQATYGFELQVP
                     EVPFPRISMADAVKALKAVGYELPAEKKGDLDPGGERALADSIKKEYQHDFFFLTDWP
                     IGVRPFYHMRHPDDETLTRSFDLIANGLEIATGAQREHRFAVLKEQALEKGLGLENIQ
                     FYLDFFRFGCPPHGGFGMGLSRLLMVLLQLQNIREAVYLFRGPNRLNP"
     gene            complement(6700..7665)
                     /gene="meaB"
                     /locus_tag="PLL88_08810"
     CDS             complement(6700..7665)
                     /gene="meaB"
                     /locus_tag="PLL88_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOJ01705.1"
                     /translation="MGELTQAVLQGDRRALARLISLVEDDTAQGRQALDVLFAKSGKA
                     HVVGVTGASGAGKSSLVNCLVDTMLKQEPEKRVAVIAVDPTSPFSGGAVLGDRIRMRA
                     IAENPNVFIRSMASRGALGGLAHNTQAVALALDAAGYDLIVIESVGAGQSEVEIASLA
                     HSVIVVEAPGLGDDVQAAKAGIMEIADILVLNKADKADVDVAEMALRSALAMGYSAAE
                     ADTSRWVPPILRTVATDGEGISEVVKTLHAHAAFLKRSGDWQQRDQARLRAFLRGLLE
                     RSREREWQQLMQKKENQGVMEQVFKRKLSPFAAIEQFEGRSILKN"
     gene            complement(7677..8078)
                     /locus_tag="PLL88_08815"
     CDS             complement(7677..8078)
                     /locus_tag="PLL88_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HOJ01706.1"
                     /translation="MEEKKIRVLIAKPGLDGHDRGAKIIARALRDAGMEVIYTGLRQT
                     PEMVVQAALQEDVDVIGLSILSGAHMSLVPRILELKKQAKLDDVQIFLGGIIPEADAK
                     ALVKQGVKAVYGPGTPTTQVVEDIRTMFEKA"
     gene            8160..9314
                     /locus_tag="PLL88_08820"
     CDS             8160..9314
                     /locus_tag="PLL88_08820"
                     /inference="COORDINATES: protein motif:HMM:NF037564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HOJ01707.1"
                     /translation="MKKISLLLILVTVLLASCQADAIAPNPLAESAGIAETTATTAPT
                     GQLSPTGTAAPAVMLNPLTGLPVSDPSILDRMPVMVKVSNYPRLGRPHAGLSFADIVF
                     DYYIGSGTNRFLAIYYGKDSPSVGPVRSGRLVDAELVRLYWGVLAYGSADEDTDAYLV
                     QALGERAISYQEAPCPAFCGTDTHSVVGVFASSADISAFARQNGIDPGGTPDLEGMVF
                     SEQVPVGGVPVEQVNILFNYYNRAEWKYNAASGTYLRWIEEMIGDEEDGNFTMLPLVD
                     RVTGEQLAFDNVIILFAHYIENAPSEHEIEIMKNIEGKRAVFYRDGYMFEGLWKVPSD
                     SAPIQFFDVAGQPFALKPGNTWVVIADDNSIFNQKASGAWELFFMLSPDA"
     gene            9405..>9678
                     /locus_tag="PLL88_08825"
     CDS             9405..>9678
                     /locus_tag="PLL88_08825"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOJ01708.1"
                     /translation="MIPIRDTQRAARFPIMNWLIIITNVLVFMVEYFSSADFSNLFVS
                     AFALTPARVDLSNPITLLPFLTHMFLHGSWYHLISNMWFLMIFGDNI"
BASE COUNT         2204 a         2702 c         2231 g         2541 t
ORIGIN      
        1 gtagatatgt ggatcaccat caacgaaccg gatgtgtaca gtttcagcgg atacatgacc
       61 ggtgaatttc cacccggcaa gagcgacccg caagccgctt tccgcgtgtt ggcgaatctg
      121 gtgcgcgggc atgctatggc atatcatgcc attcacgccc tccaaccgcg cgccctggtc
      181 ggttttgcca aaaactaccg ctccatcgtg cctgccagaa cctggttccc gcccgatcat
      241 tggatcagga atttttctca caatgctttt aacaacgcct ttgccaacac attattggat
      301 gggaaattgc ggttcatcct ctggaaagaa tccatccccc aggccatcgg aacgcaggat
      361 tacattggcg tcaattatta cacacaggat aatatcaaat ttaatgtatt gtccgcaaag
      421 agtttctttc aggagcgttt ctttacaccc aacgctgaac tgagcgaaaa tggcttcatt
      481 gccaccgaac cggatgggtt tgctgaatcg ctgaaatggg cacgacgcta ccgtgtgccc
      541 atctacatca cggaaaatgg ggtggaagat tcgcacgatg ccatgcgccc gcactacctg
      601 gtcagccacg tcctcgaaat gtggaaagcc atccaaaatg gaatggacat ccgtgggtac
      661 atgcactggt cgcagatcga taatttcgaa tgggaacgcg cctggacgca gcgttttgga
      721 ttgtggggct tggacgtgga aacccagcaa cgcatccacc gacccagcgt ggatatctat
      781 cgtgcgatct gtgaacaaaa cgccatccct gtagaaaacg tcttgcgtta tacctccgag
      841 ttgaaagctc ttgaggaatg gtacgacacc ggttatggaa atttcagcaa gaaaaattga
      901 tggatcaaac ccaaagggtt atccttcgtc ctcctgatcc ttaacagcgg gcaggcatgc
      961 gccatcgctc ttccgccgat cgaataaaaa agaagtcagt tcaatatgct gacctccctg
     1021 ctttaaagct caccccagta cagcagcgat accaccgcca gagctatccc gacgatgcag
     1081 gcatagctcg ccagcatgat attggctttg ttgcgcgtgt ctggttcctt tttcgattcg
     1141 cgccggtaca ggataactcc cacgccaaag aaaaggatcg ccggaataaa agaatacata
     1201 gcattacctc tgtgtattca ttgtacgata attccagacc atcatagaat cggtcagggg
     1261 gtgttcctca tcccgcgatc tttttttgac aggatgggca aaagtgcgtc ccgcgttgcc
     1321 ccaccacgat ccgttcgatg gaagctccgc aagtcgcgca cgcttcccct tttcgtccat
     1381 atacgcggaa ctggttttgg aagtctccgc cccggtatat ccaatcgatg ctcgccccat
     1441 tgcgccggat gccttcctgc agcgtctcct ggatggctct caataaggtt gcggcttgtt
     1501 ctccgcttaa tgcgctcgcc gtgctcaacg ggtgcagttt tgcccggtgc agggcttcat
     1561 ccgtgtaaat gttccccagc cctgccagaa aggtctggtc catcagcagc ggtttgagtt
     1621 ggcgcttgct gccttgcagc attcgataga actcctccgc tttgaagttt tccgagagcg
     1681 gctccggacc cagcttgccc agctcttctt cggggtcagt cagaagccac accctgccaa
     1741 attttcgcgc atcttcaaag accaaagatt tttcattttc cagccacaag atcacgcgat
     1801 catgcttggc gggtatctgg ttgccatcct gtaccgctgc gggtcttaca tacaggtcac
     1861 cgctcatccg taggtgaaac agcaaggtat cagtagaaag acgcactaca agatattttc
     1921 cccgccgtcc tgtctctcgg atggtttgac cgcagattcg ctggctgaat tcctgtgcgg
     1981 agggaacagc gatagaacgt tcccatagaa ttgagacatc cctgatcata tgtcccagga
     2041 tggaaaaccc cgctcccctt ccttgactga gcgaacgggc aatggtctca acttccggta
     2101 attctggcat tatatttcat caatcttgat atctagattt ccactattga agttgactaa
     2161 tttattccaa tcaatttcat agttgttatt acaaaatttt tcaataaatt cttttgtaaa
     2221 tcgattggtg attttgcttt tttcgttatg ttcctttgct ttatagccta tcggttcaat
     2281 aatattgaga tataaagatt ccatcccact gataaaaaac cagaagtttt gtcccactag
     2341 tttccaataa ttatgtaaat agctcgttcg agttttacca tagcatatac ctaagattgc
     2401 ttgaacgttt aaagatttgt ctaactgcct taaaatcgct gatgctttat tgaagtcttc
     2461 aatttgcctt ttctgttgtg aagaatttcc ccaatttgtg ccagacttta ttgaaacaag
     2521 ataatgttta ttatcacgta aaatttcaag atcaatccca tttgctgatg acttatggcc
     2581 gttacttgtc ttcgatgtga taaaaactgc cagaccttct aggaaatctc cgaaaatttt
     2641 ctcttctgat gaagatagaa acgcatcgag tagacttcca atgaggtcgc ttgcgacatt
     2701 aatatttttt gctttaaata agtacggatt ttttcttaac agcttactta gttttagaga
     2761 agaaatacat tccaaacgag tagtatgaaa acattgaatg ttctcattaa cataattgtt
     2821 aatttcagaa atttcaactt tttgcatttg tttgcccatc ctatttgtaa tttgactgtt
     2881 cttctgcaac gcttggaatt cgaatttgaa cccctgataa tctctctttc gataattcta
     2941 tataattctc tacaacatca attccaacat aattcctacc taactccata gcagctaaac
     3001 aggttgtacc tgaacctaca aatggatcta aaactaaatc tccgggttca gtcaataatt
     3061 ttataaacca tttaggcaat tcaacaggaa aaacagcact atgattttta ttggaacact
     3121 ctgttgcgag gtacaaaaca tttgtcggat aaacactctt tcttccgacc caattcgaaa
     3181 tattcttccc gaatccactg tttacttttg actcatctct cgttaaatca gtttcgctta
     3241 aatttgaaag tctatcttta gcccaattac caattggcac catgacagta tcttgaaaaa
     3301 ttttgaagtc ctttcctttt gtaaattgta acaaccgctc ccaactatcg cgaaaacgat
     3361 tcggccattt tcccggaaaa gaattttttt tatgccaaat gtattcttcc gtccacaacc
     3421 agccttgttt tttcaacgct aaaatcaact caagaacata ggtatgcctc tcacccttca
     3481 ctactctttc cttgatgtta agaacgaatg aaccggttgg tttgagtact cttttaaatt
     3541 caagggtctt aggtaaaaac cattcaacat atttatctgg gtgaattcca ccataagtat
     3601 tttttctttg atctgcatag gggggagaag taaatattaa atcaacacaa ttatctggga
     3661 gattttttaa aacctcccca cagtctccca ggattatttt atttacatat tccataattt
     3721 ttaattatag aacattattg ctatagagtg aaatgtttat tcgttgaaca tctcgcctac
     3781 cgagcgccct tcgttcaccc gacggatcac ctcaccgatc aaatttgcca ccgagatgat
     3841 gcgcagccgt ccgccgaatt gggcttgctt ttctttcgag atcgggatag tgtcggtagt
     3901 gatgaattgc ttgaccggca atttcgccat ttgtttcacg gcgtcccctg ccaggatggg
     3961 atgaatgaac accaccacca cgtccttcgc cccattcttg gtcgccacgt tgactgcgtt
     4021 gatgatggtt tttccggtgg aaacctcttc atccagcaag atcacgtctt tccctttgac
     4081 gtccccgatc agcgtcagca cctccgggtt gtccgggctc aaaatgcgcc gcttttcgat
     4141 gaacgccagc gggatgttga gctgttcggc gtagttgcgc gccttcttgg caaagcccag
     4201 atcaggggaa agtaacaccg ggcttttcat ttttttcatg atttccagca ggtaatcgat
     4261 caacacatgc gaggcataca acacatctcc cgggatcgag aagaagccct ggatttggtc
     4321 ggcgtgcagg tcgagcgtca tatagcggtc cgcaccggct gcctggatca tgtctgctac
     4381 caggcgcgcg gtgatgggca cgcgcggctg atccttttta tcggaacggg aatagcacat
     4441 gtaaggaaat accgctgtga tgcgccccgc cgaatccaat cgcagggtct ggatcaatat
     4501 cagcatttcc atgaggttgg tatgcaccgg tgaagaaagc gtttgaatca catagcaatc
     4561 ctgcccgcgc acgctagtgt gcaatttgac gaacagattc tcgtttggaa acgtcgtaat
     4621 ggtggttttg cagagtgata tttccagata agaagcgatg ctctccgcca attcggggca
     4681 ggcggagcct gcatacaatt tgacccctcc gtacatcatg cgatcatgcc gcaaactgct
     4741 ggtagtcata ctcacttttt tcctctccat tcatcttcta gaatgcccat gtacaagatg
     4801 tcgatatatt cgccatccag gaaatacgct tcgcgcatac tgccctcgtg ccggaagccc
     4861 agtttttcgt agcagtggat gccgcgctgg ttgggcgcga acacgcgcag gtagatgcgg
     4921 tgaaagttca aggtcttgaa tccatagtca agcatcagtg cgatggcttt tgcgccgatt
     4981 ccctttcccc acaacgcttt ttcgccgatg aacaaaccca gctcggcatt gcgcgattgt
     5041 tggtccacat tgagaaaact gatgttcccc aatggcttcc acgttccatc ctccacggtt
     5101 tcgatcatca aaggttgttc agtcagggga cgtttggcaa ggtctgcgaa ccattgttct
     5161 tcctgtgcgt tcgaaagcgg gctgtacaac gaaagatacc gccgcacatc cgcatcgttc
     5221 aaccagctca caaaaagcgg cagatcttcc ttgtcgattc ctctcaagcg taccgtgtca
     5281 cctatgatca tcgtttttcc tctatgggtt caggcggttc ggtccgcgga acagatagac
     5341 cgcctcgcgg atgttctgca actgcaacag caccatcagc aagcgcgaga gccccatgcc
     5401 gaaccctcca tgcggagggc agccaaagcg gaagaaatca aggtagaact ggatgttctc
     5461 caatcccaat cctttctcaa gggcttgctc cttcagcact gcaaagcggt gctcgcgctg
     5521 tgcgccggtg gcgatctcca acccgttcgc gatcaggtca aagctgcgcg tcagggtttc
     5581 atcatccggg tggcgcatgt gatagaaggg gcgtacccct attggccaat cggtcaggaa
     5641 gaagaaatca tgttgatatt ccttcttaat ggaatccgcc agcgctcgct ccccaccggg
     5701 gtcgagatca ccttttttct cggctggcag ttcgtaaccc accgccttca gggctttcac
     5761 cgcgtccgcc atgctgatgc gcgggaaagg cacctccggc acctgcagtt cgaacccgta
     5821 agttgcctgg atttgttcgc cgttcttctc tttcacctgc tggtaggcat attgcaacca
     5881 gcgttcggtg aacgccatca catcttcgtg cgaatcgatc cacgatatct ccacatccaa
     5941 tccggtgaat tccgtcatgt ggcgtgacgt gaaggaaggg tcggcgcgga agaccggacc
     6001 cacctcgaaa acccgctcga acccggctgc catgcccatc tgtttgtaga actggggcga
     6061 ttgtgccaga taggctttgc gctcaaaata ttccagttgg aacagctccg caccgctttc
     6121 gctggggctg cccatgatct tgggcgaatg gatctccacg aagtcatgct gcagccagaa
     6181 ttcacgcatg gcatgctcca gcgtagtctg cacttcgaaa atgagcagat tctgcgggcg
     6241 acgcaggtcg agatagcgcc aatccatgcg gaaatccacc gccggggtgt ttgcgtcatc
     6301 aaagggatca tacggcatgg gaatttccgc cgcgttttcc accgtcaagc tctgcaactg
     6361 gatctccaac ccgcccaatt tcaccacatc attcttgacc actttgccgg tgatggtcag
     6421 ggctgattcg acccctagtg tggagatctg gttagccaac tcttcgttgg acggtcggta
     6481 gtgagccacc tgcaccagac cggtacgatc gcgcaggatc aggaactgca tttttttctg
     6541 gtcgcgcaag atctgcagcc agccctgcac ctttacttct tgcccgatat gattgcttag
     6601 atcaccaata gaaatacgct gcatgataac tgcttctcct taaaataaat gcacttttta
     6661 agattgtaat gcgaatttgc cttcacgggg gtgctaatgt caatttttaa ggatggaacg
     6721 accctcgaat tgctcaatgg ctgcgaaagg ggagagcttt cgcttgaaca cctgctccat
     6781 gaccccctgg ttttcttttt tctgcatgag ttgttgccat tcccgttcac ggctgcgttc
     6841 cagcaacccg cgcaagaatg cccgtaagcg tgcctggtcg cgctgctgcc aatctcctga
     6901 cctcttcaga aatgctgcgt gtgcatgcag tgtttttacc acctcgctga tcccttcgcc
     6961 atctgtggca accgtgcgca gaatgggtgg cacccacctg ctcgtatcgg cttccgcggc
     7021 tgaatagccc atggcaagcg cggagcgcaa tgccatctcc gccacatcca catccgcttt
     7081 gtcggctttg tttaaaacca ggatgtcggc gatttccatg atgccagctt tggcggcttg
     7141 cacgtcatcc cccaatcccg gcgcttccac cacgatcacg ctgtgtgcca ggctggcgat
     7201 ttccacctcc gattgccccg cccccacact ttcaatcacg atgagatcat acccggcagc
     7261 gtccagcgcc agcgcgaccg cctgggtatt gtgcgccagc ccgcccagcg cgccgcggct
     7321 tgccatcgaa cggatgaaca cgttggggtt ttcagcgatg gcgcgcatgc gaatgcgatc
     7381 ccccagcacc gctcccccgc tgaaggggct ggtcgggtcc accgcgatca cagccactcg
     7441 tttttctggc tcctgcttga gcatggtatc caccaggcag ttcaccaggc tcgatttccc
     7501 cgccccgctt gcccctgtga cgcccaccac atgcgccttt ccgcttttcg cgaaaagcac
     7561 atccagcgcc tggcgcccct gagcagtgtc atcctccacc aggctgatca ggcgcgccag
     7621 cgctctgcga tctccttgca aaactgcctg cgtcagttca cccatgcacc gctcggctat
     7681 gccttctcaa acatcgtgcg gatatcctcc accacctgcg tggtgggcgt tcctggtcca
     7741 tacacggctt tcaccccctg cttcaccagt gctttggcat ccgcttcggg gataataccg
     7801 cccagaaaaa tctgcacatc atccagcttt gcctgttttt tcaactccag gatgcgcggc
     7861 accagcgaca tatgcgcgcc ggaaaggatc gataagccga tcacatccac atcttcctgc
     7921 agggctgcct gcaccaccat ttcaggggtc tggcgcagac cggtgtagat gacctccatg
     7981 ccggcgtcgc gcagcgcccg tgcgatgatc ttggcgcccc ggtcgtgccc gtctaaacca
     8041 ggtttcgcaa tgagcactcg tatctttttc tcttccatca cgtcctcttg cttcaattat
     8101 acttgcggga acgctcgctt ttttcgttat aatgcacctg tgcaacgatg atgaatagca
     8161 tgaaaaaaat atccctcctg ctgattttgg tcacagtgct cctggcgagt tgccaggctg
     8221 atgcaattgc ccccaaccct cttgcagagt ccgccggaat cgcagaaacc accgccacca
     8281 cggcacccac cgggcagcta tcccccaccg gcacggcggc acctgccgtg atgctcaatc
     8341 ccctcaccgg tttgccggtt tccgatccgt ccattttgga tcgcatgccg gtcatggtga
     8401 aggtgtccaa ctacccgcgc ctcggcagac cgcatgccgg tctttccttt gccgatatcg
     8461 tcttcgatta ttacatcggc agcggcacca accgctttct ggcgatttat tatggaaagg
     8521 atagccccag cgtcggtccg gtgcgctccg ggcgactggt ggacgccgag ttggttcggc
     8581 tgtactgggg cgtgctagcc tacggcagtg cagacgagga taccgacgcc tacctggtgc
     8641 aggcattggg ggagcgtgcc atttcttatc aagaggcgcc ttgccccgct ttctgcggca
     8701 ctgataccca cagcgtggtg ggcgtcttcg ccagctccgc cgatatcagc gcatttgcca
     8761 gacagaacgg cattgacccg ggtggcaccc ctgacctgga aggcatggtt ttcagcgaac
     8821 aggtgcctgt cggtggtgtt cccgtggaac aggtcaacat ccttttcaac tactacaacc
     8881 gcgccgaatg gaagtacaac gccgcctccg gcacctactt gcgctggatc gaggagatga
     8941 tcggcgacga agaggacggc aacttcacca tgctcccgct ggtcgatcgc gtcaccgggg
     9001 aacaactggc gtttgacaat gtcatcatcc ttttcgcgca ctatatcgag aacgccccca
     9061 gcgaacatga gatcgagatc atgaagaaca ttgagggcaa gcgcgcggtg ttctaccgcg
     9121 atggatacat gttcgagggg ttgtggaaag tacccagcga cagcgctccc attcagttct
     9181 tcgatgttgc cggtcagccc tttgcgctca agcctggcaa cacctgggtc gtcatcgccg
     9241 atgacaactc cattttcaat caaaaagcga gtggcgcctg ggaacttttc tttatgctct
     9301 ctcccgacgc ttgacggcta taattacagc atctctgctt tcatagatac ttcttatttg
     9361 tcagagcgta cagaactgct atactgttcg agaaagagag acctatgatt cctattcgag
     9421 atacccaacg tgccgcccgc tttccgatca tgaactggct gatcatcatc accaacgtgc
     9481 tggttttcat ggtagagtat ttttcctccg ctgatttttc taacctgttc gtttccgcct
     9541 ttgccctcac ccccgccagg gtagatctca gcaaccccat caccctgctg cctttcctca
     9601 cccacatgtt cttgcatggc agttggtatc acctcatcag caatatgtgg tttctcatga
     9661 tctttggcga taacatcg
//
LOCUS       DAOSDS010000080         9358 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2736_length_9358_cov_5.676556, whole genome shotgun sequence.
ACCESSION   DAOSDS010000080 DAOSDS010000000
VERSION     DAOSDS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9358
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2736_length_9358_cov_5.676556"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLL88_08830"
     CDS             complement(<1..710)
                     /locus_tag="PLL88_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01709.1"
                     /translation="MQSSQFHHKDLGIVAGSLALVLSILLSACGSSTKLPAPTSQAPV
                     ISETPLQTPTQQIAFSLSGTWTGVAKNDDLVMQVTIILQPVCQVGDICGTYDISLPCS
                     GSFVLVGEKDGIYEFRAVDKSASCTGEGKDFLQLLPNGNLEYVSRGVYGETLGELVRS
                     HTISSTPETTAQKTLIFDDDGSPDGTSALLYLLVQPNVDLKGVGISYEEAHPAIYIQH
                     IGRMLDHFGFPDILLGAG"
     gene            complement(1125..2048)
                     /locus_tag="PLL88_08835"
     CDS             complement(1125..2048)
                     /locus_tag="PLL88_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ01710.1"
                     /translation="MVTEEVVFTNETVQLVGTLYKPDIQAAHPALVVLHAANGGTRQF
                     PFYQHLVTHLPPCGIAVLLFDRRGSGHSGGNFETADFETLAEDGKAAVNYLRSRDDIN
                     KNQIGLYGISQGGWIAPLVASLRSDLAFLIIVSGCGVSPAKQMDYGAAYNLHQQGFTE
                     SAIAQAIALRNRVNEYYRGHISREVVSAELDSAQPEAWFQEAYINSSKDLPEDVTKTK
                     WWHELDYDPLPIWKQIQQPTLFLFAEDDQWVPVAESTSKLKLVTAHIKDRTIMQIKGT
                     DHLMYKIHHKKAAISEEYIETMVAWLMKRIG"
     gene            complement(2364..2765)
                     /locus_tag="PLL88_08840"
     CDS             complement(2364..2765)
                     /locus_tag="PLL88_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOJ01711.1"
                     /translation="METPRHWRLQKQRLALVGEVCPHCHAKIFPARDVCPECGEEAKT
                     AFKFSGKGEVYSFTTLHSVPAGFEENAPYTVALVKLEEGPIITAQLTDVGDREVKIGT
                     PVEMVTRRIRQDGDERGMLIYGYKFRPVLSH"
     gene            complement(2843..3994)
                     /locus_tag="PLL88_08845"
     CDS             complement(2843..3994)
                     /locus_tag="PLL88_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOJ01712.1"
                     /translation="MEKVFIAGVGQVAVGEHWEKTLSHLSTQAIRAVLRDAGSPQPEI
                     VYIGNFLAASISHQANLGALVCAEAGLRGVEGTTVEAGEASGAAALHMGYLAIGSGMA
                     DCVVVVGVEKMTDMIGNQVDAFAVQSGDTDFEGMQGLTYNGIAGLLMQRYLDQYQTPR
                     ESFASFPITAHRNGVNNPFALYRKALDLESYQKAPMVSVPLSLYDVPSYADGAAALVL
                     VSRKMLRDEQKAVLVSASEVATDTLALHDRADALAFEAVRISTANALEKAGLTRDCLD
                     LFECWDAPSIYGMLSLEAGGFVERGQGWHCATLENLALNGRLPIATLGGIKAHGFAPG
                     AAGVYQVVDAVQQLRGEAGQNQIASAQVAMTQALSGAAASVVTHILRKG"
     gene            complement(4002..5066)
                     /locus_tag="PLL88_08850"
     CDS             complement(4002..5066)
                     /locus_tag="PLL88_08850"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HOJ01713.1"
                     /translation="MGENVLLKPARAVGIVGYGVYIPQYRLPAGEILRIWAGEEGGAP
                     VKEKAVAGLDEDTITISIEAARNALARAGIDPLELRAVWVGSESHPYAVKPTSTIVAE
                     AVGAPVHIQAADLEFACKAGTEAMVAAMGLVGSKMGEYTLAIGADTAQGKPGDALEFT
                     AASGGAAFIIGPAENSLALIQAAYSYVTDTPDFWRRQHQVYPEHGQRFTGEPAYFKHV
                     TAAAQTMLEQLGTTPADYAFAVFHQPNAKFPQRVAKELGFSKEQIQTGLLSPVIGNTY
                     AGASLVGLTAVLDIAKPGDRILVVSYGSGAGSDAFDIEVTDLIEERRKKAPSTRQYID
                     RRVEIDYATYARYRGKIVMK"
     gene            complement(5069..6346)
                     /locus_tag="PLL88_08855"
     CDS             complement(5069..6346)
                     /locus_tag="PLL88_08855"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HOJ01714.1"
                     /translation="MPDEKKFYQMSIQERLQLLQSACALSDEELAVFSPQGGLSSVQA
                     DLMIENVVGVYALPLGIAQHFLINGRPVLVPMAVEEPSVVAAASYMAKLTQAGGGFSA
                     TAGAQEMIGQLQILQVKDIRAVERALLDAREELLTLAAEADPLLVKVGGGPKDLQVRI
                     IQDSPLGSFLVLHLILDVQDAMGANAVNTALEHISSRVEQITGGRVLLRILSNLADRR
                     LAKASCTIPLEALAFENYKGEEVRDRIIEAWAFAAADPYRAATHNKGIMNGVDAVVIA
                     SGNDWRAVEAGAHAYAARSGRYTSLSTWSVDKSGNLRGELEMPMAVGVVGGATRVHPT
                     ARTVLKIMNVRKASDLAEIIVSVGLAQNLGALRALATEGIQRGHMALHARQVAVAAGA
                     PPEKVAALAEQLVKEGNIRVDRAIAILNEWSNL"
     gene            complement(6424..7110)
                     /locus_tag="PLL88_08860"
     CDS             complement(6424..7110)
                     /locus_tag="PLL88_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOJ01715.1"
                     /translation="MVAKLTFYGHASFDLQVDALHLLFDPYLSGNPTASISAEAVPAD
                     YILVSHGHGDHLGDAVKIAKRSGATVITVAEIADWLREQNVKTHAQHLGGGFQYPFGY
                     CKLTLALHGSKLPDGSHGGNPAGFLITLNDGKKIYFAGDTGLFGDMRLIGEEGVDVAL
                     LPIGDNYTMGPADALRAVELICPKVAIPMHYNTFPLIKQDAHAWKKLVEEKTSTRVIV
                     LQPGESYSLA"
     gene            7235..8344
                     /locus_tag="PLL88_08865"
     CDS             7235..8344
                     /locus_tag="PLL88_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOJ01716.1"
                     /translation="MSDPRVQKLATMLVDYSLGVKANQRVLLQGEKGGEPLMMAIFNA
                     CLERDAIPFIFQYPEGMIDSYFRYGKAEKFAKYLEQLKTFVETYDHRVRILGESNTKE
                     LSGFDPDLVSKFTAEGGKISRIMLDREQKGEMHWVLSLFPTPAYAQDANMSLPDFEDF
                     VYNACMPDQNDPVSYWKKVAARQDKIIQWLKGKERVHVTAPETDLTLSIKGRPFENCA
                     CKVNVPDGEIFTSPVEDSVNGHVYYSYPTMYEGFEVSGVRLWFENGKVVKASAQKNEE
                     FLMKKLDTDAGSRYLGEFAIGTNEGIQKFTGQILFDEKIGGSFHMALGHGYPETLSQN
                     ESSIHWDMICDLRNGGKIEVDDELLYENGKFVIDF"
     gene            8446..9315
                     /locus_tag="PLL88_08870"
     CDS             8446..9315
                     /locus_tag="PLL88_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOJ01717.1"
                     /translation="MKMRLGIAQIDTKLGVVDANLQKHLEYIERARQGGVDLLMFPEL
                     SLTGYVLQDLVPAVAHRPKPDDPIFKELFKASHDLDLLIGFVEEDERNRFYIADAYLS
                     RGELVHTHRKVYLPTYGLFDEGRFFAWGDTTQAFDTRFERSGVLICEDFWHIAPPYLL
                     WLDGADLLLFTSASPGRGLTEAPQLSTSMWVESINRAYASIFTSFVAHCNRVGFEDGL
                     HFWGGSTVYDPDGNCLIKAPYDEEAFLTVELDLNQLHRTRARLPLLRDERTALVQREL
                     NRILSNGHSNSGK"
BASE COUNT         2289 a         2621 c         2243 g         2205 t
ORIGIN      
        1 atcccagcgc caagaagaat atcaggaaaa ccgaaatgat caagcatcct gccgatatgt
       61 tgaatgtaga tagcgggatg cgcttcctca taggatatac cgacgccctt taaatcgaca
      121 ttcggttgaa cgagcaaata taacaaagcg gatgtcccat ccggactgcc atcatcatcg
      181 aatatcaggg tcttttgagc tgttgtttct ggggtggaag agatagtatg gctacgaacc
      241 agttctccaa gcgtctcgcc gtagacgcct cgactgacat attccaaatt gccattgggc
      301 aataattgaa gaaagtcctt cccttctcct gtacaggaag ccgacttgtc aacagctcga
      361 aattcataaa taccgtcttt ctcaccgact agaacgaaac tcccggaaca aggcaaacta
      421 atatcatatg tcccgcaaat atctccaacc tgacaaaccg gttggagtat gatcgtgacc
      481 tgcataacaa ggtcgtcatt cttcgcaacc ccggtccacg ttccggaaag agagaaggcg
      541 atttgctgag ttggagtttg taaaggagtt tcactgatga ctggagcttg agaagtaggc
      601 gcaggtagtt tggtggaaga accacaggct gacagaagaa tgctcagtac gagagcaaga
      661 ctgccagcga caatacccaa gtctttatga tggaactgtg aactctgcat cggattctct
      721 ctgaacgaat ttgtaaagtt tgaataaaca tgccgttcaa taattctttt aatttgttat
      781 ttccttgccg aaaccggtca gctacctttt caatgatcca agaaaacagt atttcaattc
      841 ctataaattt taactctttt ttgtttctac ataataaaaa agcgtcctcc aattcaggaa
      901 ggcacttcat cattcgtact ttaaccttac cgcatcacca gccgaaactt acacttgtac
      961 atacgacctg gcagatcaga gttattcgct gacgtttaca ctactggcac cgggggtttg
     1021 gacaacattt gcgagcagga cgctgttcgg gcgcagaact gcccgaaaat gacgacgaat
     1081 cccacctatc cagtgcatgt cttgttggcc ggcacttcta aactttaacc aatgcgtttc
     1141 atcaaccaag caaccatcgt ctcaatatat tcttctgaga tggctgcttt cttgtggtgt
     1201 attttgtaca ttaagtgatc tgttcctttt atctgcatga ttgtcctatc tttaatgtgc
     1261 gcagttacca gcttcaactt ggatgtactc tctgcaactg gcacccattg atcatcttca
     1321 gcaaatagaa aaagcgtagg ctgttgtatt tgcttccaaa tgggaagcgg gtcgtagtct
     1381 agctcgtgcc accacttagt tttcgtaaca tcttctggca aatccttgct tgaattgata
     1441 taggcttcct gaaaccatgc ttcgggttgg gcactatcaa gttcagcaga aacgacttcg
     1501 cgagaaatat gccctcgata atattcattt acgcggttac gcagagcaat tgcttgggct
     1561 atagcgcttt ctgtaaagcc ctgctgatgc agattatagg ccgcgccata atccatttgt
     1621 tttgcaggtg aaactccaca tcccgataca atgattagaa atgcaagatc agatcgcaga
     1681 ctagcgacaa gaggtgcaat ccagccacct tggctgatac catataatcc aatctggttt
     1741 ttattaatat cgtcgcgtga gcgcagataa tttacggcgg ctttaccgtc ctctgccaaa
     1801 gtttcaaaat cagccgtttc aaaatttcca ccagagtgac cggatcctcg ccgatcaaag
     1861 agcaacacgg ctatgccaca tggcggtaaa tgcgtaacca ggtgttgata gaaagggaat
     1921 tgccgagttc cgccgttagc cgcatgaaga actaccaagg caggatgggc agcttgtata
     1981 tctggtttat aaagcgttcc cactaactga acggtttcat tcgtaaagac tacttcttct
     2041 gtaaccatat tcctaattac cccaacgtga gtataagctt tcccgtacct gtcgcccaat
     2101 ggttcgcgtt agtggcggta ggggcgcgga caaagcttgg gagcagagaa aactcaaata
     2161 ttttcataga agagatgtgt gtcgaaaata atccaggtat gggagaaata atgagtgtat
     2221 tattgttatt gggaaacctc tttttaggta aggtactatg ttttattcca caacaaatac
     2281 cacatataaa gctaaccatc tatgcatact tgaacagaaa ccaccattct tacgtgggtg
     2341 gcaatttttg tttttgacat ttctcaatga gataaaaccg gtctgaactt atacccgtaa
     2401 atcaacatgc cgcgctcatc accatcctgg cggatgcggc gggtaaccat ctccaccggc
     2461 gtgccgatct tcacctcacg atcacccaca tccgtgagct gcgcggtaat gatgggacct
     2521 tcttccaatt tgacaagtgc taccgtatag ggcgcatttt cttcaaaacc ggccggaacg
     2581 ctgtgcagcg ttgtgaagga atatacctcg ccttttccac tgaacttgaa cgcagtcttg
     2641 gcttcttcac cacattcggg gcatacatcg cgcgcgggga agatcttggc atggcaatgc
     2701 gggcatactt cgcccaccag ggcaaggcgt tgtttttgta atctccaatg tctgggtgtt
     2761 tccatttatt tatgctcact ccttcaagag agtgaaacaa gtgtacaaat ggctttctct
     2821 caaaagctat caatttcttc ggctagcctt tgcgcaaaat atgggtgacg acactggctg
     2881 ctgcgccgct gagcgcctgc gtcatggcaa cttgcgcgct cgcgatctgg ttctgcccgg
     2941 cttctccgcg caactgttgc acggcatcca ccacctgata gacgcccgcc gcaccagggg
     3001 cgaagccatg cgctttgatg ccgcccaggg tggcaatagg cagccgacca ttgagtgcca
     3061 gattttcgag ggtggcacaa tgccaaccct gcccgcgctc gacaaacccg cccgcttcta
     3121 aggaaagcat gccgtaaata gaaggagcat cccagcattc gaagagatcc agacaatcgc
     3181 gcgtgagtcc ggccttttcc agagcattgg ctgtgctgat gcgcaccgct tcgaaagcca
     3241 acgcatctgc ccgatcgtgc aacgccagcg tatcggtggc aacctccgat gcgctcacca
     3301 gcaccgcttt ttgctcgtcg cgcaacatct ttcgactcac cagcaccaac gctgcggctc
     3361 catcggcata ggatggcacg tcatacaggg aaagaggcac gctcaccata ggcgctttct
     3421 ggtaactttc caggtccagc gcctttctgt atagggcaaa aggattgttt accccattgc
     3481 ggtgagctgt gatgggaaaa ctggcaaagc tctcgcgggg cgtctggtac tgatcgaggt
     3541 agcgttgcat gagcaacccg gctataccat tgtacgtaag tccctgcatg ccttcgaaat
     3601 cagtgtcccc gctctgcaca gcaaaggcat caacctgatt cccaatcatg tcggtcattt
     3661 tttccacccc cactaccacc acgcaatccg ccattcctga accaattgcc aggtaaccca
     3721 tatgcaaggc agccgcacca gaggcttcac ccgcttccac ggtggtaccc tctacacccc
     3781 gcaagccagc ctcagcgcac accagcgctc ccaaattggc ttgatgggaa atggaagcag
     3841 ccagaaaatt gccaatatat acaatttcag gctgtgggct acccgcatcg cgcaacacgg
     3901 cgcggatcgc ctgtgtgctg agatgactga gcgttttctc ccagtgttcc cccaccgcca
     3961 cctgaccgac gcctgcaata aagacttttt ccatacttgc tctacttcat cacgatcttg
     4021 ccgcggtagc gcgcgtaggt ggcataatcg atctccaccc gtcgatcgat atattgtcgg
     4081 gtggagggcg ctttcttacg ccgctcttcg atcagatcgg tcacctcgat gtcaaaggca
     4141 tctgagccgg cacctgatcc atatgaaacc accaatatac gatcgcccgg ttttgcgata
     4201 tccaacactg cggtcaatcc caccagtgat gctccggcat aagtgttgcc gatgacgggt
     4261 gagagcagcc cggtttggat ctgttccttg ctgaagccca attctttggc aacccgttgc
     4321 ggaaacttgg cattgggttg gtgaaaaacc gcaaaggcat aatctgcagg cgtggtgcca
     4381 agctgctcaa gcatggtttg cgccgctgca gtaacatgct tgaagtaagc cggttctccg
     4441 gtgaagcgct gcccgtgttc cggataaacc tgatgctggc ggcgccagaa atcaggagta
     4501 tcggttacgt acgaatacgc cgcctggatg agtgccaggc tgttctctgc cggaccgatg
     4561 atgaaagccg cacccccaga cgccgcggtg aattcaagcg catcgcccgg cttgccctgc
     4621 gcggtatctg cgccaatagc cagggtatat tcccccatct tcgaaccgac cagacccatg
     4681 gcagccacca tggcttcggt gccagccttg caagcgaatt ccagatcagc agcctgaatg
     4741 tgcaccggcg ctcccaccgc ctctgccaca atggtcgagg tgggtttgac cgcataggga
     4801 tgcgattcag aacccaccca caccgcacgc aattccagcg gatcgatgcc tgctctggct
     4861 aaggcgttgc gcgctgcttc aatggaaatg gtgatagtgt cctcatccaa ccccgccacg
     4921 gctttctctt tcaccggcgc accgccttcc tcgccagccc agatgcgtaa aatctcgccg
     4981 gcgggcaaac ggtattgggg gatgtacacc ccatacccca caatgcccac cgcccgggca
     5041 ggtttcaaca atacattctc acccatagct acagattgct ccattcattc aaaattgcga
     5101 tcgcacgatc tacgcgaata ttcccttctt ttacaagctg ctctgccaaa gctgccactt
     5161 tttcaggcgg tgcgccagcc gccaccgcca cctgccgggc atgcagtgcc atgtgcccgc
     5221 gctggatgcc ttcagtagcc agagcacgta aggcgcccag attctgcgcc agcccaactg
     5281 agacgatgat ctccgccagg tccgatgctt tccgcacgtt catgatcttc aggacagtgc
     5341 gcgcggttgg atgcacgcgg gtggctccgc ccacaacgcc caccgccata ggcatttcca
     5401 gctcaccccg caaatttcca cttttgtcaa ccgaccaggt gctcaagctg gtgtagcgcc
     5461 cgctgcgggc agcatacgca tgcgccccgg cttccacggc gcgccagtca ttcccgctgg
     5521 cgatcaccac cgcatccacg ccattcatga tgcctttgtt atgggtggct gcccggtagg
     5581 gatctgccgc ggcaaatgcc caggcttcga taatgcggtc acgcacctct tcacccttgt
     5641 aattctcaaa agccagcgct tccagcggta tggtgcagga ggctttggct aatcgcctgt
     5701 cagccaggtt ggaaaggatg cgcagcagca ccctaccgcc ggtgatctgc tctacccgcg
     5761 agctgatgtg ctccagcgcg gtgttgaccg cgttggcgcc catggcatct tgcacatcca
     5821 gaatgaggtg cagtaccaga aagctcccaa gaggagaatc ctgaatgatg cgcacttgca
     5881 agtctttcgg acctccgcca actttcacta acaagggatc agcctctgcc gccaacgtca
     5941 gtaactcctc cctggcatcc agcagcgcgc gttccaccgc ccggatatct ttgacttgca
     6001 gaatctgaag ctgcccgatc atctcctgcg cgccggctgt tgcactgaag ccaccacccg
     6061 cttgcgtcag cttagccatg taggatgccg cagccaccac agaaggttct tccaccgcca
     6121 tgggcaccaa caccgggcgc ccgttgatta aaaaatgctg ggcaatgccc agcggcaaag
     6181 cgtacacccc caccacattt tcgatcatca aatctgcctg cacggaagat aagcccccct
     6241 gcgggctgaa cacagccagc tcctcatcgc ttaaagcaca agctgattgc aacagctgca
     6301 ggcgttcttg tatactcatc tggtagaact ttttttcatc aggcattgtt caacgctccc
     6361 ttcaccaagg aaggcaccgc tcaatcatac agaatcagtt cttgcaaaag ctatcagatc
     6421 cgatcaagcc agagaatagc tttcccctgg ctgcagaacg atcaccctgg tgctggtttt
     6481 ttcctccacc agttttttcc aggcgtgcgc atcctgtttg atcagcggaa aagtattgta
     6541 gtgcatgggg atagcgacct tggggcagat cagttccacc gctcgcagcg cgtctgccgg
     6601 acccatggtg tagttatcgc cgatgggcag aagtgccaca tccacacctt cttcgccgat
     6661 caggcgcata tcgccaaaca aaccggtgtc gccggcaaaa tagattttct ttccatcgtt
     6721 cagcgtgatc aaaaagccag ccgggttgcc gccgtgcgag ccatccggca atttggagcc
     6781 gtgcagcgcc agggtcaact tgcagtaccc aaaaggatac tggaaccctc ctcccaggtg
     6841 ctgcgcatga gttttcacgt tctgttcgcg caaccaatcg gcaatctctg ccaccgtgat
     6901 cacggtggcg ccgctgcgtt tggcaatctt cactgcatcc cccagatggt caccatgccc
     6961 atgactcacc aaaatataat ccgccggcac tgcttcggca ctgatgctgg cagtgggatt
     7021 gccgctcagg taggggtcga aaagcagatg caacgcatct acctgcagat caaagcttgc
     7081 atgtccataa aaggtaagtt ttgcgaccat atcatcctcc ttgaaactca tttcttttct
     7141 tattttaatg aaggacgttt gaaacttcca tatttatcga ttttccatat acaataaaga
     7201 gcgatacgag aaaaccagtt tcaaggagca aatcatgtct gatccgcgtg tacaaaaatt
     7261 agccaccatg ttggtggatt attctcttgg cgtcaaagcc aaccagcgcg tgctgctaca
     7321 gggcgaaaaa ggcggcgagc cactaatgat ggcgattttc aacgcctgtc tggaacgcga
     7381 tgcgatcccc ttcatcttcc aatatcctga aggcatgatc gacagttatt ttcgctatgg
     7441 aaaagctgaa aaattcgcaa aatatcttga acagcttaaa acctttgtag aaacctatga
     7501 tcaccgcgtg cgcattttgg gcgaatccaa caccaaagag ctttccggct tcgatcctga
     7561 cctggtttca aaattcaccg ctgagggcgg caagatttcg cgcattatgc tcgatcgcga
     7621 acaaaaaggc gaaatgcact gggttttatc gctgttcccc accccagcat atgcgcagga
     7681 tgccaacatg agcctgcccg attttgagga cttcgtctac aacgcctgca tgcccgacca
     7741 gaatgacccg gtgagctact ggaagaaagt agccgcccgg caagataaga tcatccaatg
     7801 gctgaagggc aaggaacggg tgcatgtgac cgctcctgaa accgacctca ccttgagcat
     7861 caaaggcaga ccttttgaaa actgtgcctg caaggtcaat gtaccggatg gggagatttt
     7921 cacctcaccc gtcgaagatt cagtaaacgg acatgtctat tattcctacc ccaccatgta
     7981 cgagggtttt gaagtgagcg gggtgcgcct gtggtttgag aacggcaaag tggtcaaagc
     8041 cagcgcccaa aagaacgaag aatttttgat gaaaaaactg gacaccgatg ccggttcgcg
     8101 ctacctgggc gagttcgcca ttggcaccaa tgaaggcatt cagaaattca ccgggcagat
     8161 cctctttgac gagaaaatcg gcggcagttt tcacatggcg ctcgggcacg gctatccgga
     8221 aaccctcagc cagaacgaat cctccatcca ctgggatatg atctgcgacc tgcgcaacgg
     8281 cggaaaaatc gaagtggatg acgagctgct gtacgaaaac gggaaattcg tcatcgattt
     8341 ctaacctgac gcatcataaa agtcaaaagg gcgcaggtca aatttgcgcc cttttctttt
     8401 aagttacaat actaaagaaa gaacaaaatc aagcaggata atcctatgaa aatgcgcctg
     8461 ggaattgcac agatcgatac caaactgggt gtggtggatg caaacttgca gaaacacctg
     8521 gaatacatcg aaagagcccg tcaaggcggg gtagatctat tgatgttccc cgaactctcc
     8581 ctcaccggat acgtgctgca agatttggtt cccgccgtag cgcaccgtcc caagccggat
     8641 gatccgatct tcaaagaact tttcaaagcc agtcacgatc ttgatctgct gatcggtttt
     8701 gtggaagagg acgaacgcaa ccgcttctac attgcagatg cctacctctc tcggggtgag
     8761 ttggtgcaca cgcaccgtaa agtatacctg cccacctatg gattgttcga cgaggggcgg
     8821 ttctttgcct ggggtgacac cacgcaggca ttcgacacgc gcttcgaacg cagcggggtg
     8881 ctgatctgcg aagatttctg gcacattgcc ccgccgtatt tgctgtggtt ggatggggct
     8941 gacctgctgc tcttcacctc ggcttctccc gggcgcgggc tcaccgaagc accacaactt
     9001 tctacctcca tgtgggtaga gagcatcaac cgcgcttatg ccagcatctt cacttctttc
     9061 gtggctcact gcaaccgggt aggtttcgaa gacggactgc atttctgggg aggctctacc
     9121 gtttacgatc cagacggcaa ctgcctgatc aaagctccat acgatgaaga agccttcctc
     9181 accgttgagc tggacctcaa ccagttgcac cgcacgcgcg cgcgcctgcc gttgctgcgc
     9241 gatgaacgca ccgcccttgt gcagcgcgaa ttgaaccgca ttctttccaa cggacactcg
     9301 aattcaggga aataagggag gaatgatgaa aattgacctt acgatcaaca cagatctg
//
LOCUS       DAOSDS010000081         9298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2758_length_9298_cov_4.759169, whole genome shotgun sequence.
ACCESSION   DAOSDS010000081 DAOSDS010000000
VERSION     DAOSDS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9298
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2758_length_9298_cov_4.759169"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="PLL88_08875"
     CDS             <1..188
                     /locus_tag="PLL88_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01718.1"
                     /translation="LNPGSVGQPRDHDPRASFAIFDTENLTWELRRVEYDISSVQDRI
                     KKAGLPWRHAMRLHEGW"
     gene            252..1160
                     /locus_tag="PLL88_08880"
     CDS             252..1160
                     /locus_tag="PLL88_08880"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOJ01719.1"
                     /translation="MKKHRILISFMLFAILLAACASRAASSEAVYNEKAYSEDMVAME
                     AAAPAEISYAAGAVPSYDVERVVIKNADLSIVVADPVSALDTISQMAEDKGGFVVNSY
                     LYKITSVSGKELPAASVTIRVPAESLDETLAEIKALVEDAEIDILSESVSGQDVTSEV
                     TDLESRLRNLQQAEEQLLEIMDAATDTEDVIAVFEQLTSIRGDIEVLQGQLKYYEESA
                     KLSAVSISLKAKEEVAPITIGGWRPSLTIQRAIQALVDGLKFIANALIWIVIFVAPIV
                     LVIGLPIYLIIRASKKRRTVQKKTEK"
     gene            complement(1208..3553)
                     /locus_tag="PLL88_08885"
     CDS             complement(1208..3553)
                     /locus_tag="PLL88_08885"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HOJ01720.1"
                     /translation="MNHTQNPFLALSIDLHSGAFTIEPSDSDFPAIRKAQLVITVKSD
                     ERHFQIPLTLREDTAHETAEKPRTSSRRAVITFHPSSPIPGLDVQVLFSIPFNQPAIF
                     WKVKILNQGSTPLHIEKIDLVRRPSGSKSPCLRVNGSPDALSFYVNGWQSWSFSALTS
                     RLEPQMRSRLGFLQNPMIVDAGLRRAGSFASLADMYAVLFNRDKEKGCLLGFLSQKEQ
                     FGHIALTAWKDADVCMWADADSVRLDEQTELQTDWATFMLVEGLDNSSIDLYLDEVAR
                     ENAVAFQKKVFTGWCSWYQYYHDISRETIGENLKVLVDHRQDFPLDLVQIDDGFQTKV
                     GDWFSFTAGFPQGVLPLARDIQQNGFLPGLWLAPFIVDRSSSLFRDHPDWILRKATGS
                     PVNAGFGWNALTTALDLTQPAASEYVREVISTAVGDWQYPYLKLDFLYAAALKGKYYD
                     PTKTRAQVLRAGLETIRTAAGQQIYLVGCGLPIGSGIGLVDSMRVGPDVAGSWVPKFE
                     DVKIRFTREPSVPSARNSLRNILSRANLHDRWWVNDPDCVLVRDQSELTLPEVQTLAT
                     VVAMSGGSMIFSDDLTALSDERLWLASCLLPPVTDRLLVLDWLQHQYPHRLRMNLRGP
                     AGDWVLLAHINWLDVTIPLSLSCSAFGLADGNYWISDFWAKKIHKNTDLSSLGEFVVP
                     AHGCALLAVRPVQRGEQYLGSDLHFSQGKEVAKWSSRRPATRFTLDAGRHTSGSIFLS
                     LPKAPKNITSSGDILNCTMLQQGVYELQVQLEQPQTITITH"
     gene            complement(3564..5660)
                     /locus_tag="PLL88_08890"
     CDS             complement(3564..5660)
                     /locus_tag="PLL88_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01721.1"
                     /translation="MKTKKIPFEILLLAVILVLHAFVAFSPEERLLNWFQTDDAFYYF
                     VTARNIAEGRGITFDGISLTNGFHPLWMLICIPVFSLARVDLYLPLRIIVMILALLNA
                     ATGILLYRFFAKKFSQETGWFVAIFWTFLPTIHELTSKLGLESGLTAFALAAALTYLS
                     SFEERELKPVNYLTMSALGLLLLFSRLDTIFIVILMGIWLVFRKTTLRWQVLLDGLAG
                     FLSALSAYYLRVQNTDNIFNFLPFLYIFMVLSLVSKVVVQYMLKGYLCDSSVSLKKQI
                     LNTVLALGISSVLTGGVLFVLHDILHVFLGFPRVVLLIDLAIALVLFLAWRIGYIVYM
                     RFKKRDYTEDISWRANGKTWLVNAVCYFGPILVLLAVYMVFNKSYAGAYMPISGTIKR
                     WWGTLPLTVYGQPLKTLGKVLGSWFDPTTKEGPWWLVTAPVSLFIEIVARATGLPQPS
                     LVFNNFKRDFGVLVWALVLILIAWLIYRKKGFIQKAVRESAFLPLLIGCIFHLFSYKA
                     TGYLHAKYWYWIPEVFCTVIFMGIFVESVLRRYKRYLHGIFASQLLIGTFCLVLVANL
                     FVPTVRQYAYRDDAAEPHQYILETQFVEDHSKPGDVIGMTGGGVIAYFIEDRTIVNLD
                     GLVNGVEYFEQLKTARAYQYLDSIGLDYVFAAKSMILESDPYGWIFSGRLAYLDKRGQ
                     FNFYRYERGVQTEVNP"
     gene            complement(5759..7672)
                     /locus_tag="PLL88_08895"
     CDS             complement(5759..7672)
                     /locus_tag="PLL88_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOJ01722.1"
                     /translation="MQKERKPFRTTRLITQKPVPSDIAIAQAAQLRPISEVAEDLGIL
                     PQELELYGPYKAKVKLEVLERLKDLPDGKYIDVTAINPTPLGEGKTTTTVGLSQALGA
                     HLQKRVMTCLRQPSQGPTFGIKGGAAGGGYSQVIPMEDLNLHLTGDIHAVTAANNLLA
                     AAVETRLYHENHQTDDALFRRLYPQDKDGTFQVGRGQLGRLKRFGIDPRQLGSLSAEQ
                     KARLVRLDIDPQHIYWMRGIDTNDRFLRGITVGQGPQEVDSERKTGFYITVASEIMAV
                     LALATSIEDMRERLGKMVVALNKKGEPVTAEDVGIAGALTVLMADAIKPNLLQTLEGT
                     PVFVHAGPFANIAHGNSSILADRIGLKLADYVITESGFGSDMGMEKFFDIKCRYSGLK
                     PNVVVLVATVRALKMHGGGPKVTAGQVLDRAYKEENLDLLRNGLDHLFHHIGIVHQYG
                     VPTVVAINRFVTDSDSELELVQRSVRDAGMADDCVVTNHWEMGGAGAVELAEAVIKAA
                     ERPSGFKFLYPLDWSIKKKIESIVSQVYGADGVDYAPEAEKKIEEFTRLGYADLPVCM
                     AKTHLSLSHDQELKGVPKGYRFPIRDIRLSAGAGFLYPLAGEISTMPGLPTRPAFMDI
                     DIDPLTGKISGLS"
     gene            complement(7682..8863)
                     /locus_tag="PLL88_08900"
     CDS             complement(7682..8863)
                     /locus_tag="PLL88_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ01723.1"
                     /translation="MFIDRLGGSLLYPFFSLYITQRFGVGMTEVGYIFMLHTSAMFFG
                     NMLSGALTDKFGRKKMLLAGIILSGTSSLLMGFIPDYETFIIFTVITGLLTNIGGPAV
                     NAMMADILPERQRAQGFSILRVAVNLSVSFGPAIGGLLAGYNFFYLFVADFVISMLTA
                     GIVFLKVPETKPQSTPDPLVPEQNLLKTLGGYGLIGKDWFFMGILFLSLLTNLVYMQM
                     SSSLSVFLRDMYSISSQKYGYILSLNAVMVVVMQFWVTRQISGYRPMLLMFFGNILYA
                     IGFGMYGFVGTYPLFLLAMAIITIGEMISSPIMQSIIAHLAPQHMRGRYMAVFNLSYG
                     TANAIGPLAAGIIMDNYDPNWVWYAGGIICTVSALGYLLMQKPAKKKLVSLAQLDEQK
                     E"
     gene            complement(8933..>9298)
                     /locus_tag="PLL88_08905"
     CDS             complement(8933..>9298)
                     /locus_tag="PLL88_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ01724.1"
                     /translation="MALATLLYGVGFSMIGFVDRYWMFMVAMAIITLGEMVHIPTSSG
                     LIANLAPEDKRGRYNAFAGLSWGVPNIFSTFFAGLVMDNLNPDLVWTIAGGICLVTLC
                     GYLLMNTQVQKRLASLTQE"
BASE COUNT         2572 a         2480 c         2260 g         1986 t
ORIGIN      
        1 tcctcaaccc cggttcagtg gggcagccgc gcgatcatga cccgcgggca tctttcgcga
       61 ttttcgatac agaaaactta acctgggaac tgcgcagggt ggaatacgat atcagcagcg
      121 ttcaagaccg gatcaaaaaa gccgggttgc cctggaggca tgctatgcgc ctgcacgaag
      181 gttggtagca gggaactttt taccggtcgc aacgtttaaa aggcaagcct aattttttct
      241 aaggaggaaa aatgaagaaa catcgtattt tgatcagctt catgctcttc gccatcctac
      301 tggcagcctg tgcttcaaga gcggctagct cagaagcggt ctacaacgaa aaagcatata
      361 gtgaagacat ggtcgccatg gaagctgctg ctcccgcaga gatttcctac gctgctggag
      421 cggtgccatc gtatgacgtg gaacgcgtag tgatcaaaaa tgccgacttg agcatcgttg
      481 tagcagatcc ggtgagcgca ttggatacga tcagccagat ggcagaagac aagggtggct
      541 ttgtagtgaa ttcctatcta tacaagatca cttctgtatc cggcaaagaa ctgcccgcgg
      601 cttcggtgac cattcgcgtt cccgcagaat cgttggatga aacgttggca gagatcaaag
      661 cgctggttga agacgcagag atcgacattc tgagcgaatc ggtatcagga caggacgtta
      721 ccagcgaagt gaccgacctg gaatcgcgcc tgcgcaacct gcaacaagct gaggaacaac
      781 tgctcgagat catggatgcc gccaccgaca cagaggatgt gatagcagta ttcgagcaac
      841 tgacctccat ccgcggggac atcgaggtgc ttcaaggtca attgaaatat tatgaagaat
      901 cagccaagct ttctgcagtt tccatcagct tgaaagcgaa agaagaagtt gcacccatca
      961 ccatcggggg atggagacct tcgctgacca tacaacgcgc cattcaggct ttggtggatg
     1021 ggctgaaatt catcgccaac gcgttgatct ggatcgtaat cttcgttgct cccatcgtgc
     1081 tggtgatcgg actgccaatc tatctcatca tcagagcctc aaagaaacgc cgcacggttc
     1141 aaaagaaaac agagaaataa ggatacgaga aaaggctgac caaaaataaa agggtcagcc
     1201 tcttttttta gtgggtgatc gtgatggttt gcggctgttc taattgcacc tgcagctcat
     1261 agacgccctg ctgcagcatt gtacagttta aaatatcgcc tgaagaggtg atattcttag
     1321 gagccttggg gagagaaaga aagatgctcc ctgatgtatg ccgacccgca tccaaagtaa
     1381 aacgcgtggc aggtctgcga gatgaccact ttgccacctc cttgccttga gaaaaatgca
     1441 ggtcagaacc gagatattgc tcaccgcgct gcacgggacg cacagccaga agcgcacaac
     1501 catgcgccgg aacgacgaac tcgcccagcg aagagagatc tgtattcttg tggatctttt
     1561 tcgcccagaa atcactgatc caataatttc catcggcaag cccaaaggcg ctgcaagaaa
     1621 gagacagcgg tatcgttacg tccagccaat taatatgcgc cagcaaaacc caatcccctg
     1681 caggtcctcg cagattcatg cgcaagcgat gcggatattg atgttgcagc caatcgagca
     1741 caagcaggcg atcggtcaca gggggaagca ggcatgacgc cagccacagg cgttcatcag
     1801 acaacgcagt cagatcatcg gaaaagatca tagagccgcc gctcatcgcc accacggttg
     1861 ccaacgtttg cacctcaggc agggtcaact ctgactgatc gcgcaccaga acacaatcag
     1921 ggtcattcac ccaccagcga tcatgcaaat tagcgcggga caagatgttg cgaaggctgt
     1981 tgcgggcact cggtacgctg ggttccctgg taaagcggat tttgacatcc tcaaacttcg
     2041 gaacccacga accagccaca tctggtccaa cgcgcatgga atccaccaaa ccgatccccg
     2101 agccaatcgg caagccacac cccaccaggt agatttgctg cccagcagcc gtgcgtatgg
     2161 tttccaatcc agcccgcaac acctgcgcac gggttttggt ggggtcataa tatttccctt
     2221 taagggctgc tgcgtataaa aagtccaatt tcaaataagg atattgccag tctccaaccg
     2281 ccgtgctgat tacctcacgc acatactcag aggcagcagg ttgggtgagg tcgagggctg
     2341 tggtgagggc attccaaccg aaacccgcgt tcaccgggct gccagtcgct tttctcagaa
     2401 tccagtcagg atgatccctg aacaggctgg aagagcggtc tacgataaat ggcgccaacc
     2461 acaaaccagg aagaaagcca ttctgctgga tatcgcgagc aagcggtaac acaccttgcg
     2521 gaaaacccgc cgtgaatgaa aaccaatcac caacctttgt ttggaatcca tcatcgatct
     2581 gcaccagatc aagtgggaaa tcttgccgat gatcgacaag caccttcagg ttttcgccaa
     2641 tggtctcacg gctgatatca tgataatact ggtaccagga acaccagcca gtgaacactt
     2701 ttttttgaaa agcgaccgcg ttttctctgg cgacctcatc caggtagaga tcaatgcttg
     2761 aattatccaa gccctccacc agcatgaacg tcgcccaatc ggtctgcaat tctgtctgct
     2821 catccaggcg cacgctatcg gcatccgccc acatgcaaac atccgcatcc ttccacgccg
     2881 tcagtgcgat atgcccgaac tgctcttttt gggaaagaaa gcccagcaga cagccctttt
     2941 ctttgtcacg attaaaaaga acagcgtaca tgtcagcaag gctggcaaag gaaccggctc
     3001 ttcgcaagcc ggcatccaca atcatggggt tttgcaaaaa gccaagccgc gagcgcattt
     3061 gcggttctag ccgcgaggta agcgcactga aagaccagga ctgccaacca ttcacataaa
     3121 aagacagcgc gtcagggctc ccattcaccc gcaaacaggg gctcttgctt cctgacggtc
     3181 ttctcacaag gtctatcttc tcgatatgga gcggcgtgga accctgattc agaatcttta
     3241 ccttccagaa aatagcaggc tggttgaaag ggattgaaaa aagaacctga acatccaacc
     3301 ccgggatagg agaagatgga tgaaaagtga tcacagcgcg ccgtgaagac gtacgaggct
     3361 tttctgcagt ctcgtgtgcc gtatcttctc gcaatgttaa cggaatttga aaatgcctct
     3421 catcactttt cacagtaatg acaagctgtg cttttctgat tgccggaaaa tccgaatcgg
     3481 aaggctctat ggtgaacgct ccgctgtgca aatcaattga aagagccagg aatggatttt
     3541 gtgtatggtt catctcatca ttttcagggg ttaacctctg tctgcactcc cctctcatag
     3601 cggtagaaat tgaattgacc tcgtttatcc aggtaagcca gacgccctga gaagatccag
     3661 ccgtagggat cgctttccag gatcatcgat ttggctgcaa acacataatc caacccaatc
     3721 gaatcaaggt actgataggc acgcgcggtt ttcagttgtt caaaatattc aacaccgttg
     3781 accaagccat cgaggttcac gatggtgcga tcttcgatga aataagcgat cactccccca
     3841 ccagtcatcc cgataacatc gcccggtttg gaatgatcct ccacaaactg cgtttcaagg
     3901 atatattgat gcggctcggc agcatcatcg cgataggcat actgtcgaac ggtgggaaca
     3961 aagagatttg ccaccaagac cagacaaaaa gtgccaatca gcagttgcga tgcgaatatt
     4021 ccgtgcagat agcgcttata acggcggaga acgctttcga caaagatgcc catgaaaata
     4081 accgtgcaga agacctcggg aatccaatac caatacttgg cgtgcaaata gccggtggct
     4141 ttataactga acaggtggaa aatacaaccg atcagcagtg gaaggaaagc gctttcacgg
     4201 acagcctttt gaataaagcc tttctttcga taaatgagcc aggcaatgag tatcagcaca
     4261 agagcccaaa ccagaacccc aaaatcacgt ttgaaattat tgaaaaccag ggacggttgg
     4321 ggcaaaccgg tggcgcgagc cacgatctcg ataaagaggg acaccggagc ggtaaccaac
     4381 caccagggac cttctttggt cgtagggtca aaccagctac caaggacttt tccaagggtt
     4441 ttcaagggct gcccgtatac cgtgagcggc agcgtacccc accagcgttt gatggttccg
     4501 ctgataggca tataggcgcc agcataggat ttgttgaata ccatatatac agccagcaga
     4561 acaagaatcg gcccaaaata acaaacagca ttcaccagcc aggttttgcc gttggcacgc
     4621 catgaaatat cttctgtata atcccgtttt ttgaagcgca tgtaaacaat atagccaatt
     4681 cgccaagcca aaaagaggac caaagcaatc gcgagatcaa tgagcaacac cacgcgcggg
     4741 aatcccagaa aaacatgcaa gatatcatgc aacacgaaca gcaccccacc cgttaaaacc
     4801 gatgagatgc ccaaagcaag cacagtgttc aggatctgtt ttttcaagga taccgaggaa
     4861 tcacataaat accctttcaa catatactgg accaccacct tggaaacgag ggagagaacc
     4921 atgaaaatgt agagaaaggg caggaagttg aagatattat cggtgttttg gacgcgcaaa
     4981 taataggcgc tcagggcaga aaggaagccc gccagcccat ccaacaacac ctgccagcgg
     5041 agagtggtct tgcgaaacac cagccagatg cccatcagaa tcacaataaa aatggtatct
     5101 aaccggctga aaagcaagag cagccccaaa gcgctcatcg tcaaatagtt caccggtttg
     5161 agttcacgct cttcaaaaga ggacagataa gtgagggctg ccgccaaagc aaaagcggtc
     5221 aagccagatt caagacccag tttggaagtc aactcatgaa tggtcggcag gaaggtccaa
     5281 aaaatcgcca caaaccagcc ggtttcctgc gagaactttt tggcaaaaaa acggtacagc
     5341 aggatgcctg tggcggcatt caacaacgcc agaatcatga caatgatgcg caaaggaagg
     5401 taaagatcca ccctcgccag gctgaacaca gggatacaga tgagcatcca taaaggatgg
     5461 aagccattgg tcagactgat cccatcaaag gtgatacccc gcccttcagc gatattgcga
     5521 gcagtaacaa aataataaaa agcgtcatct gtctggaacc agttcaacaa gcgctcttca
     5581 ggagagaaag ccacaaaagc atgtaaaaca agaatgacag ccagcaacaa gatctcaaag
     5641 gggattttct ttgttttcat tttcctgtcc attcacggat gtgggtttat tatagcgcat
     5701 ggcaaataaa gagaaggcag atgatgaaac gctgtcttct ctttatcatt tcagcctgtt
     5761 acgacaaccc gctgattttg cccgtcagcg gatcgatgtc gatatccatg aaagccggac
     5821 gggtcggcaa gcccggcatg gtgctgattt cgccggctaa aggatacagg aaacccgctc
     5881 cggctgaaag gcgaatatcg cggatgggaa accgataccc tttcggcacg cctttcagct
     5941 cctgatcatg gcttaagctc aaatgggtct tcgccataca aacaggcaaa tccgcataac
     6001 ccaggcgggt aaattcctcg atcttctttt ctgcttcagg ggcataatct accccgtctg
     6061 caccatacac ctggctgacg atggattcga tcttcttctt gatcgaccaa tccagcggat
     6121 agagaaactt gaaaccagac ggtctttccg ccgctttgat caccgcttca gccagttcca
     6181 ccgcgccagc cccgcccatc tcccagtggt tggtgacgac gcagtcatcc gccatgcctg
     6241 catcgcgcac agatcgttga accaactcca actcagaatc agaatctgta acaaaccgat
     6301 tgatcgccac cacagtggga acaccgtatt ggtggacgat cccaatatga tggaaaagat
     6361 ggtccaaacc attgcgcagc aagtccaggt tttcttcttt atatgcccga tccaacacct
     6421 gaccggcagt gacctttgga ccgccgccat gcattttcaa agcccgcacg gtggcaacca
     6481 gcacaaccac attaggtttc agccccgaat aacgacactt tatgtcaaag aacttttcca
     6541 tgcccatgtc cgagccaaat ccggattcgg tgatgacata atcggctaat ttcaagccta
     6601 tccggtcagc caggatggag ctatttccat gcgcaatgtt ggcaaagggc ccggcatgca
     6661 cgaagaccgg cgtgccttca agggtctgga gcagattagg cttgatggca tctgccatga
     6721 gcacagtcaa cgctccggca atacccacat cttctgcggt gaccggttcg ccttttttat
     6781 tgagagcgac caccatcttc cccagtcgtt cgcgcatatc ttcaatgctg gtagccagcg
     6841 ccaacaccgc catgatctca ctggcaaccg tgatgtaaaa cccggtcttt ctttcggaat
     6901 caacttcctg gggaccctgc ccaaccgtga tgccccgtaa gaagcgatcg ttggtatcaa
     6961 tgccgcgcat ccagtaaata tgctgcgggt cgatatccag gcgcaccagc ctggcttttt
     7021 gctcagcaga tagcgacccc aactggcgtg gatcaatgcc gaatcttttc aagcgcccaa
     7081 gctgccctcg cccgacctga aatgtgccgt ctttatcttg cggatagagc cggcggaaca
     7141 aagcatcgtc agtttggtga ttttcatggt acaaacgcgt ttccacagcc gcggctaaga
     7201 gattattggc tgctgtgacg gcatggatat caccagtgag gtgcaggttc agatcttcca
     7261 tcgggatgac ctggctatac ccgccaccgg cagccccgcc tttaatgcca aaggttggac
     7321 cctggctggg ctggcgcagg caggtcatca cacgtttctg caagtgcgcc cccaacgcct
     7381 ggctgagccc gacggtggtg gtggttttgc cttctcccaa tggggtgggg ttaatggcag
     7441 tcacatcaat atacttgcca tccggcaggt ctttcaggcg ctccaacact tcgagcttca
     7501 ctttcgcttt gtaaggacca tacaactcca attcctgcgg caaaatgccc aaatcttccg
     7561 ccacctcact gatgggacgc aactgcgccg cctgggcaat cgctatatcg ctcggaacgg
     7621 gtttctgggt gatcaaacgt gttgtgcgaa aaggttttcg ttctttctgc attttcatcc
     7681 ctcattcttt ctgctcatcc aattgagcta ggctgaccaa cttctttttc gccggcttct
     7741 gcatcagcaa atagcccaac gcagaaacgg tacagatgat gccaccggca taccaaaccc
     7801 agttgggatc gtaattatcc ataatgatac cagcagccag cggccctatc gcattggctg
     7861 tgccgtagct gaggttgaaa acagccatat atctgccgcg catatgctgt ggggcaaggt
     7921 gggcgatgat agattgcata atgggcgaac tgatcatctc accaatcgtg atgatagcca
     7981 tcgccaggag gaaaagggga taagtgccga caaagccata cattccaaag ccaatggcgt
     8041 aaaggatatt tccaaagaac atcaacagca tcgggcggta tccactgatc tggcgggtga
     8101 cccaaaactg catcaccacc accataacgg cattgagcga aagaatatag ccgtatttct
     8161 gcgaagagat actatacata tcccgcaaga agaccgagag gcttgaactc atctgcatat
     8221 acaccagatt ggtcaacagc gatagaaaaa ggatgcccat gaaaaaccaa tctttcccga
     8281 taagtccgta cccgccaagc gtcttcagca agttttgctc aggtacgagc gggtcaggcg
     8341 ttgattgcgg cttggtttcg gggaccttca agaacacgat gccggcggta agcatactga
     8401 tcacaaaatc agccacgaac agatagaaaa agttatagcc agccagcaga ccaccaatag
     8461 caggaccaaa ggacaccgaa agattcaccg caacgcgcag gatgctgaac ccctgggcgc
     8521 gctgccgttc ggggaggata tcagccatca tggcgttcac ggctggaccg ccgatattgg
     8581 tcaacagacc ggtaatcacg gtgaagatta tgaacgtctc gtaatctgga atgaatccca
     8641 tcagcaggct gctggttccg ctcaagatga tgccagccag cagcattttc ttgcgaccaa
     8701 atttatcggt caacgccccg ctcagcatgt tcccaaagaa catggcggag gtatgcagca
     8761 tgaatatata cccgacctcg gtcataccca cgccaaaacg ctgggtgatg tacagtgaga
     8821 agaacgggta taacagcgac cctcccagcc gatcaataaa catcgctcct atgagggtcc
     8881 aaaatgtatg ggaaaagcct ttatacgttt ctttgatgct ctggaacaat tcctactcct
     8941 gcgtgagcga tgcgaggcgt ttttgtacct gcgtgttcat caataaataa ccacacaaag
     9001 tcaccaagca gataccacct gcaatagtcc acaccaggtc aggattcaga ttgtccatca
     9061 ccaaacctgc gaaaaaggtg ctgaaaatgt tcggcacgcc ccagctcaga ccggcaaacg
     9121 cattgtacct gccacgttta tcttcagggg caaggttcgc gatcaacccg ctggaggtgg
     9181 ggatgtgcac catctcaccc agggtgatga tagccatggc aaccatgaac atccaatacc
     9241 ggtcgacgaa tccgatcatg gaaaaaccaa ccccatacag cagggtagct aatgccat
//
LOCUS       DAOSDS010000082         9155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2816_length_9155_cov_5.349560, whole genome shotgun sequence.
ACCESSION   DAOSDS010000082 DAOSDS010000000
VERSION     DAOSDS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9155
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2816_length_9155_cov_5.349560"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLL88_08910"
     CDS             complement(<1..201)
                     /locus_tag="PLL88_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01725.1"
                     /translation="MTNDTERKQTYEIYPIGFVQSDEQTRRYEIELLPRFSTGLEKLE
                     DFSHVIIFWWADQHDNLEDRSIL"
     gene            complement(427..1194)
                     /locus_tag="PLL88_08915"
     CDS             complement(427..1194)
                     /locus_tag="PLL88_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOJ01726.1"
                     /translation="MEKSTVIRRNQILKMLAESEELKTSSLSDYFDVTTETIRKDLLY
                     LQNTGAVKKLHGRVRLSTKGSDTPLDLRTFQMRNEKTLIAKQAVRMVENNSTVYIDGG
                     STALEIAKLLNQKEGLVVITTSLSAANYIAKQNNTVYIASGFVEGPSMIIHGPLLFES
                     IKQFKPVISFMGTNGVLFHDGPTSKNFSDIEAKNAIVLNSNIAVVVCDSSKFSESGML
                     QYTSWSNIDFLITDSGIPAASKEQLEKQTKIIIAEEK"
     gene            1403..2530
                     /locus_tag="PLL88_08920"
     CDS             1403..2530
                     /locus_tag="PLL88_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ01727.1"
                     /translation="MNSRERVLKTINHQDADRPPMDLGGAASSLNDAAYFNLKRYLEI
                     TGDIQPFRQGTTSNYYDERILDRLNIDFRRLIAKRSERYPVFHEDGSFTNEWGIKYVK
                     QGEYYETRENPLANADESQIKHYAWPKATDIFDISGLYEEAKRMYSQTEYALVARMPC
                     WGLVDLAFQLRGMENFMLDMVTKPAITRYLIEKILEVQIDIYSLILDHIGEFVQMVET
                     CDDLGSQNNLLFSERCFREIVKPSRTALNIEIKKKAPQAKIFMHSCGAIARLIPDLIE
                     TGVDILNPIQPNASDMDPEVLKKNFGDQLCFHGCIDTQIAFRGSVADTEKEVKKKIAV
                     LYKEGGYIVAPANHIMSDVPIENIVALYRTVENYNTWREKE"
     gene            2527..3015
                     /locus_tag="PLL88_08925"
     CDS             2527..3015
                     /locus_tag="PLL88_08925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ01728.1"
                     /translation="MIIFTRIDDRVIHGQTLVCWLSDYPCDGILIIDDELAKNSVMAE
                     IYKGAVPANIRVHVFDIATARTKLPEAHASAKKYFVIFKSVLSCLKVLDVIAPLINVT
                     NVGPASKRPDTKEIVPTIALDKQEIEAYREIHKAGIKIFFQIIPTQKKVLWNELDKSI
                     TG"
     gene            3086..3859
                     /locus_tag="PLL88_08930"
     CDS             3086..3859
                     /locus_tag="PLL88_08930"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOJ01729.1"
                     /translation="MLALQAILVGLFCWLGSVCTPFMGFTLGWYVLSRPLIGGLICGI
                     IFGDIPQGILLGAVVQTAYLAVVTPGGAFPAELGFIAYPAMGLALAAKMDTGMAIALA
                     ASIGVLGTFILNFTLAINSVFNGMGDRALERGDEAGFKRAHLLYPQLLQLIVRGVPAF
                     LAVYFGAQYIEGIIASLPEFVITGLLALGGLLPAVGIATLLFQSVKERYFILFFLFGF
                     VAIVGLKLSIIQLTIVALVLAFLFYRSTVNDAKEKEEVL"
     gene            3859..4671
                     /locus_tag="PLL88_08935"
     CDS             3859..4671
                     /locus_tag="PLL88_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOJ01730.1"
                     /translation="MAAKDERLTKAELTKLWWRYMWWYNSSASFERFHAMGIVYSLIP
                     LWNKYYDKAKKFTAMKRHTHFYNTEMQTGALIHGITVGLEEQKALGKDVSDDVIRANK
                     VGLMGPIAGIGDSILVGVIIPILLSIAISLSSGGSVIGPLFYIAAYVALIVLGSKFLF
                     DRGYTLGVDAVKTLIGERAKKVVNAIILLGVTVMGGMAASYVGFTTKISYGSGEEIRL
                     LQDTLDGIFPKLLPLLLVLGIWALMAKKKVSPLIMTIILAVFAFVAAFFKII"
     gene            4739..5179
                     /locus_tag="PLL88_08940"
     CDS             4739..5179
                     /locus_tag="PLL88_08940"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ01731.1"
                     /translation="MAHLIVVSHGTFASGVVNAVEMITGKDENLDICSLDPEKGVVDF
                     QQSFIEIIKKINTPETKLLILCDLYCGCPYTTAFEITNSMLPADKFKIVSGVNLPMML
                     ETCLANQQHPDDLELLYKTALSAGHFGIHEPSIKGPLCDQDEVL"
     gene            5218..5853
                     /locus_tag="PLL88_08945"
     CDS             5218..5853
                     /locus_tag="PLL88_08945"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOJ01732.1"
                     /translation="MKLQLSLDFGRLDDAIHTLSEIHQSVDVIELGTPLLFLEGLGAL
                     TVIKELYPEIDLLADLKIIDGGSYESTLAFEAGADIVTVLGCASEKTIGAAIDTAEKF
                     RKKTVIDLLGVQQVEQKIRQIDKTKADYACLHTAMDDSKEKSVPVEELAIAKTTATRV
                     GIAIAGGINISNIQKILPYQPDIIVIGSGITQQPDKAQAAAEIKKAMNDHQ"
     gene            5840..6409
                     /gene="hxlB"
                     /locus_tag="PLL88_08950"
     CDS             5840..6409
                     /gene="hxlB"
                     /locus_tag="PLL88_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HOJ01733.1"
                     /translation="MTTSNPARIHQIILKELSDTLEKSSGDEIENLCRAIASAKRLFI
                     CGAGRTGLIMKCFAMRLMQIGLQVWVVGETTTPSINEYDLLIIGSGSGETESILSIVE
                     KARTMGVKTAVFTIFSDSSIGKGADILVQIFAPSLKSKMVSSAISRQPLGNLFESSLL
                     LVTDGIVMQLMTMLQIDQSMMFSRHANLE"
     gene            6426..7136
                     /locus_tag="PLL88_08955"
     CDS             6426..7136
                     /locus_tag="PLL88_08955"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOJ01734.1"
                     /translation="MKQSELKTTTQLSASMICMDACDLKAGIHKLEDSSIDYVHVDLI
                     DGYFSPSMPIGLDTIRCVRKITSLPFDAHVMAKENDFFVESLLDIGVQRLCFQYETEA
                     HIDHMIRKIKKAGAKAGIALKPVTPISMLEYILPECDFILLMLINPGYANNDDEKQVP
                     YAIDKVKEAREYIHKKGGKTLIEVDGRVSISCIPDLVQAGADTLVLGSSSLFCKANSF
                     EENLNQIRTAIQQAEEAE"
     gene            7136..8221
                     /locus_tag="PLL88_08960"
     CDS             7136..8221
                     /locus_tag="PLL88_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ01735.1"
                     /translation="MDVQPVERVLRAISHQQPDRVPIDLRFSPELQNGLIKELRMSEK
                     EFWEWVGQDVITIRPKFSHPASPIKYADPTIEIDPHGYYLDIYRVPFRKISNPFQDYL
                     EPEMSQAPLANLNSIEELEKFPWPSANDWDYSHIFSGISSVKHMATWSRSRGCFQTAQ
                     LMRGTEKFLIDLSLNPDYACFLMDRIMDFVIEDAKLSILASQGKYTFVEYNDDIATQR
                     GLLISPTMWRKYVKPRMKAFCDMVHGFGMKVKYHSCGSIYSVIPDLIEIGVDILNPIQ
                     TLAANMDPFKLKVEFGKDLCLHGGVDIQHLLPNGSKQEVCDYVQRLIDIVGKDGGYIL
                     AGTHTIQNDARIDNIIAMIKLAKGGNA"
     gene            8218..8793
                     /locus_tag="PLL88_08965"
     CDS             8218..8793
                     /locus_tag="PLL88_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01736.1"
                     /translation="MKMETSKIFSEKTSLIIHECDRVLKSIDPLAVERLLDEIENADK
                     VFLVGVGRVFLSLQAIAKRLNHLGIPTYCVGQVDEPPISSKDLLIVGSSSGETIIPVS
                     IAGKAHDFGAKIAYIGANHNSTVRKISNIFIPIPITKAIQHETVVASQQPMTSLFEQV
                     LLLFGDAITLMLMERMGLQESDILRKHANLE"
BASE COUNT         2603 a         2021 c         2132 g         2399 t
ORIGIN      
        1 caggatactg cgatcttcga ggttgtcatg ttgatcagcc caccaaaata tgatcacgtg
       61 gctgaaatct tccagttttt caagccccgt actgaaccgc ggaagcagtt ctatttcata
      121 acgcctcgtc tgctcatcgc tctgcacgaa tccaatcggg tagatttcat aggtctgttt
      181 tctctcggta tcgttggtca tccaatgctt ctcctttttt actgaggatg accatttcgg
      241 agcatgctta cttgatgtga attgcccaaa tgcttttgat ttgcttcgaa gaacattgat
      301 gatttgacaa aaattgctgc ggggaaatgt tcgatcagga ttttttgatt aatacctgat
      361 cagacggttg agattatctc cgatgtttaa aaaagaagaa ttttttcttc ctttacaagg
      421 ctcgcattat ttctcttcgg caatgatgat cttggtttgt ttttcgagct gttctttgga
      481 tgcggcagga atgccgctat cggtgatcaa aaagtcgata ttcgaccaac tggtgtactg
      541 cagcatgcct gattcggaaa atttgctgct gtcacaaacc accacggcaa tattgctgtt
      601 cagcacgatc gcattcttcg cctcgatatc agaaaaattc ttgctggtgg gtccatcatg
      661 gaaaagcacg ccattggtac ccataaaaga gatcaccggc ttgaactgct tgatgctttc
      721 aaaaagtaaa ggtccatgga taatcatgga aggaccttct acaaaaccac tggcaatata
      781 taccgtgttg ttctgtttcg cgatgtaatt ggctgcgctg agcgaagtgg taatgacaac
      841 cagtccttct ttttggttga gtagtttggc aatctcgagt gccgtgcttc cgccatcaat
      901 atagacggtt gagttgtttt ctaccatacg aaccgcttgt ttcgcgatca gcgtcttttc
      961 attgcgcatt tgaaaggtgc gcagatcaag cggcgtatcg ctgcctttgg tcgaaaggcg
     1021 caccctacca tgcagtttct tcacagctcc ggtattttgc agatagagca ggtctttgcg
     1081 gatcgtttca gtggtgacat caaaataatc tgacaggctc gaggtcttca attcttcact
     1141 ttctgctagc attttgagaa tctgattcct gcgaatcacc gtgctttttt ccattttcac
     1201 cctctcaatc caagcttttc attaagtcat aaatacaatg acaattcaca atttatcggt
     1261 catactttgt tcattatcgc atcttaattg cttattatac caataatttt ttgtgttttt
     1321 gcaacattcc aactgtattg actttatttt agttgcacta tattattttt ttacaatttc
     1381 gcaatattgg gttttataaa caatgaattc aagggaacga gtacttaaaa ccatcaacca
     1441 tcaagatgca gaccgccctc ccatggatct gggcggggca gcgagcagtt taaatgacgc
     1501 agcctatttc aatctcaagc gatatctgga gatcaccgga gatatccagc catttcggca
     1561 ggggacgacg tcgaactatt atgatgaaag gatactggat cgactgaata tcgattttcg
     1621 cagactgata gcaaagagaa gcgaacggta tccggtgttc catgaagacg gttcttttac
     1681 aaacgaatgg gggatcaaat atgtaaaaca aggtgagtat tacgaaacgc gagaaaaccc
     1741 cctggcgaat gcagatgaat ctcaaataaa acattatgcg tggccaaaag cgactgatat
     1801 ctttgacatc agcggtctgt acgaagaagc aaagcggatg tattcacaga ccgaatatgc
     1861 actggtagcc agaatgcctt gttggggttt ggtggacctg gcttttcaat tgagggggat
     1921 ggaaaatttc atgctcgata tggtcaccaa gcccgctatc acgcgatatc taatagaaaa
     1981 aatccttgag gtccagatcg atatttactc cctcatcctg gatcacatcg gtgaatttgt
     2041 ccaaatggta gaaacatgtg acgatctggg aagccagaat aatctcttgt tctccgaacg
     2101 ctgtttccga gaaatcgtca aaccgagcag aacagccctc aatattgaaa taaaaaagaa
     2161 agctccacag gcaaaaatat tcatgcatag ctgtggcgcg atcgcaaggc tcattccaga
     2221 tctcatcgaa acgggtgtcg atattctcaa ccccattcaa ccaaacgcaa gcgatatgga
     2281 tccagaggta ctaaaaaaga atttcgggga tcagctttgt tttcatggct gcattgatac
     2341 gcagatcgcc ttccgcggtt cggtggcaga cacggagaaa gaagtgaaga aaaaaatagc
     2401 tgttctctac aaagagggcg gatacatcgt tgcaccggcg aaccatatca tgtcggatgt
     2461 tccgatagaa aacatcgttg ccttatatcg aactgttgaa aactacaaca cctggagaga
     2521 aaaagaatga tcatttttac gcgaatcgat gaccgcgtga tccatggaca gaccctggtc
     2581 tgctggcttt cagattatcc ctgtgatggg atcctcatca tcgacgatga actagcaaag
     2641 aattccgtaa tggcggagat ctataaaggc gcagtgccag ccaacatccg ggtgcatgtc
     2701 tttgatattg cgacagcaag aacaaaacta cccgaagccc atgcttcagc caaaaaatat
     2761 tttgtcatct tcaagtctgt gctgagctgt ttgaaagttc tggatgtgat cgcaccgctt
     2821 atcaatgtga ccaatgtggg tccggcaagt aaacgccctg ataccaaaga aatcgtgcca
     2881 accattgctc tggataaaca agaaatcgag gcatatcgag agatccacaa agcaggcatc
     2941 aagatatttt tccagatcat tcccactcaa aagaaagttt tgtggaatga attagataaa
     3001 tcaatcaccg ggtaagaaaa ggaggtggct aaagacaaat agcttcgcat tttcattagt
     3061 gtttgaaaaa aataaaggag gaaaaatgtt agcattacaa gcaatactgg taggtttatt
     3121 ctgctggcta gggtcagttt gtacaccatt catgggcttc acgctgggat ggtatgtcct
     3181 ttctcgacca ctaatcggtg gattgatctg cgggatcatc ttcggcgaca tcccacaagg
     3241 aatattgctc ggtgcggtag tacagaccgc ctacctggca gtggtcacac ccggcggtgc
     3301 ctttccggca gagctgggat tcatcgcata cccggctatg ggtctggctc tagcagcgaa
     3361 aatggacacc ggaatggcga tcgcgctggc tgcctcaatc ggcgttctgg gaacattcat
     3421 tctgaacttc acgcttgcca tcaactctgt tttcaatgga atgggcgaca gggcactcga
     3481 aagaggtgat gaagccgggt ttaagcgcgc tcatctactt tatccgcaat tgctccaatt
     3541 gatcgtcagg ggtgttccgg cttttctggc ggtgtatttc ggcgcacagt acattgaagg
     3601 aatcattgcg tcactgcccg aatttgtcat cacaggatta ctggctttgg gtgggttgct
     3661 gcccgcagtg ggtattgcca ctttgctatt ccaaagcgtc aaggaaagat acttcatcct
     3721 gttcttcttg ttcggattcg tggcaatcgt cggtctgaaa ttaagcatca ttcaactcac
     3781 tatcgtcgcg ctggtattgg ctttcttgtt ctatcggtcg acagtgaatg acgcaaaaga
     3841 aaaagaagag gtgctctaat ggctgcaaaa gatgagagat taaccaaagc tgagttaaca
     3901 aaactctggt ggcggtatat gtggtggtac aacagctcag ccagctttga gcgctttcac
     3961 gccatgggca tcgtgtactc attgatcccg ctgtggaaca aatactatga caaagcaaag
     4021 aagttcacag caatgaaacg ccacacgcac ttctataaca cggaaatgca gaccggtgcg
     4081 ttgatccatg gcatcaccgt tggattggag gaacagaaag cgcttggaaa agatgtgagc
     4141 gatgatgtca tccgcgccaa caaagttggt ttgatgggtc cgatcgcagg cattggtgat
     4201 tccattctgg tgggagtcat cattcccatc ttgctctcca tcgccatttc gctttcatct
     4261 ggcggcagtg tgatcggtcc gctcttctac attgctgcat atgtggcttt gatcgttctc
     4321 ggttcaaaat tcttgttcga tcgcggttac accctgggtg tcgatgcggt gaaaaccctc
     4381 atcggtgaaa gagcaaaaaa ggttgtcaac gcgatcattt tgttgggtgt aacggtcatg
     4441 gggggtatgg cagccagtta cgttggcttc acaaccaaaa tcagctatgg cagcggagag
     4501 gaaatcagat tattgcaaga tactctagat ggcatcttcc cgaaattgct gccactgctg
     4561 ctcgtgttag ggatctgggc attaatggct aagaagaaag tatctccttt gatcatgacc
     4621 atcattctgg cggtcttcgc attcgttgca gctttcttca aaatcattta acgtaaccaa
     4681 acaatgacaa ggcatttggc aaccaaatgc cttgtcaaat tagcatagag agaagtacat
     4741 ggcgcattta attgttgtat ctcacggaac ttttgcatca ggggtggtca acgcggtaga
     4801 aatgatcact ggaaaagatg agaatcttga catctgctcc ctcgacccgg aaaaaggagt
     4861 ggttgatttt caacaatcgt tcattgaaat cataaaaaaa atcaacacac cagaaaccaa
     4921 gctcctgatt ttatgcgatc tgtattgtgg atgcccatat acgacggctt tcgagatcac
     4981 gaactccatg ctacctgcag ataagttcaa gatcgtgtcg ggagtaaacc tcccaatgat
     5041 gttggaaaca tgcctggcaa accagcagca ccccgatgat ctggaactct tatacaagac
     5101 cgcgttatct gccggtcatt ttggtattca cgaaccttcc ataaaaggac ccctttgcga
     5161 tcaagatgag gtcctttgaa ttcgctgtca atttgaaaga ataagcatag aggaagaatg
     5221 aaattacagc tctcacttga ttttggacga ttggatgatg caatccatac actttctgaa
     5281 atacaccaaa gcgtggacgt catcgaactt ggaacgccgc tcttatttct ggaagggttg
     5341 ggtgcattaa ccgtcataaa ggaactttac cctgagatcg acttactggc agatctcaag
     5401 atcatcgatg gtggttccta tgaatcaacc ctggcttttg aagcaggggc agatatagtc
     5461 actgtgctgg gttgtgcaag tgaaaaaacg attggggcag ccatcgatac ggctgaaaaa
     5521 ttcagaaaaa aaaccgtgat cgacctgctg ggggtacagc aagtcgaaca aaagatacgg
     5581 cagatcgaca aaacaaaagc ggattatgca tgcctacata ccgccatgga tgacagcaaa
     5641 gaaaaaagcg tcccagtgga ggagctggca atcgcaaaaa ccaccgccac ccgtgttggc
     5701 atcgcaatag caggaggtat caacatcagc aatatccaaa agatcctacc ctatcaaccc
     5761 gatatcatcg tcattgggtc aggaattacc caacaacccg acaaggcgca ggctgccgca
     5821 gagatcaaga aggcaatgaa tgaccaccag taacccagcc cgcattcatc agataatcct
     5881 gaaggaacta tcggatacgc tggaaaaatc cagcggggat gaaatcgaaa acctgtgccg
     5941 ggcgatcgcc agcgccaagc gtttattcat ctgcggtgca gggcgaaccg gactcatcat
     6001 gaaatgcttt gcgatgagat tgatgcaaat cggattgcag gtctgggtgg ttggagaaac
     6061 gaccacacct tctatcaatg agtacgatct gctcatcatt ggatctggct ccggtgaaac
     6121 ggagagcatc ttaagcatcg ttgaaaaagc aagaacgatg ggcgtgaaaa cagcagtttt
     6181 caccattttc tccgactctt ccatcggcaa gggtgcagat attcttgttc aaatctttgc
     6241 tccttcgctc aaatcaaaaa tggtctcctc tgccatttcc cggcagccat tgggaaacct
     6301 tttcgaatca agtcttttat tggtgacgga tggaattgtg atgcaattga tgacaatgct
     6361 gcaaattgac caaagcatga tgttctccag gcatgcaaat ctggaataag tattcaggat
     6421 aacgaatgaa acaaagcgaa ttaaaaacaa ccacgcaact ctcagcatcg atgatctgta
     6481 tggatgcatg cgatctaaaa gcgggtattc ataaattaga ggattcgagc attgactacg
     6541 tgcacgttga cctgatcgat ggctatttca gcccaagcat gccgatcggt ttagacacca
     6601 tccgatgcgt gcggaagatc acaagtttgc cttttgatgc gcatgtgatg gcaaaggaga
     6661 atgatttttt tgtcgagtcg ttactggata tcggtgtaca aaggttatgc ttccaatatg
     6721 aaactgaagc ccatatcgat cacatgatta gaaagatcaa aaaagccgga gcgaaagcag
     6781 gaattgccct gaagcccgta acgccaatat ctatgctgga gtatattttg ccggaatgtg
     6841 atttcatttt gctcatgctg atcaacccgg gttatgcaaa caacgatgat gaaaaacaag
     6901 ttccttatgc gattgacaag gtcaaggaag cccgtgaata tatccataaa aaggggggca
     6961 aaacgctaat cgaggtcgat gggcgtgtgt cgatctcctg tatccctgac cttgttcaag
     7021 cgggagcgga caccctggta ttaggatctt ccagtctttt ttgtaaagcc aacagcttcg
     7081 aagaaaatct caatcagatc cgtacggcga tccaacaagc ggaggaggct gaataatgga
     7141 tgtccaacct gttgagcggg ttctccgggc gatatcgcac cagcagccag atcgcgttcc
     7201 cattgatctg cgattttctc ccgaactgca aaatggattg ataaaagaac tgaggatgag
     7261 cgagaaagaa ttctgggagt gggttgggca ggatgtgatc acgatcagac cgaaattcag
     7321 ccaccccgct tcaccaatca aatatgctga tccaacaata gaaatcgatc cgcatggtta
     7381 ctatctcgat atttaccggg tgccgttccg gaagatatcc aatccttttc aagattatct
     7441 cgaacccgaa atgagtcagg cacctctggc aaatctgaac agcattgaag aactggagaa
     7501 gttcccctgg ccaagcgcga acgactggga ttattcccac atcttttcag gcatcagttc
     7561 agtgaaacac atggcgacct ggtcgcgcag ccggggatgt tttcaaaccg cacaattgat
     7621 gagaggcacc gaaaagttct tgatcgatct cagcctgaac cccgattatg catgtttcct
     7681 gatggacagg atcatggatt ttgtaataga agatgccaag ttatctattc tggcatcaca
     7741 ggggaaatac acttttgttg aatataacga cgatattgcc acacagcggg gattactcat
     7801 ttcaccaacc atgtggagaa agtatgtaaa gccacgcatg aaagcttttt gcgacatggt
     7861 tcatggcttt ggaatgaaag tgaagtatca ctcctgcggt tctatctatt ccgtgatccc
     7921 tgacctgatc gaaattggtg ttgatatttt gaaccccatc caaacccttg cggcgaatat
     7981 ggaccccttc aagttaaagg tggaatttgg aaaagatctg tgcttgcacg ggggagtgga
     8041 tattcagcac ttacttccga atggttcaaa acaagaagtg tgcgattatg ttcaacgctt
     8101 gatcgatatt gtgggaaaag acggcggtta catacttgcg ggtacgcata ccatccaaaa
     8161 tgatgcaaga attgacaaca ttattgccat gataaaactc gcgaagggtg ggaatgcatg
     8221 aaaatggaaa cgagcaagat attttcagag aaaacttcat tgatcatcca tgagtgtgac
     8281 cgcgtcttga aatcgatcga tccattggct gtggaacgat tgctggatga aatcgaaaat
     8341 gcggacaagg tctttcttgt tggtgttggg agagttttct tatcgcttca agcaattgcg
     8401 aaaagactga accaccttgg cattccaaca tattgcgtgg ggcaggtcga tgaacctccg
     8461 ataagcagca aggacctgct catcgttggc tcaagcagcg gtgaaacaat catccccgtt
     8521 tcgatcgcag gaaaagccca cgatttcggt gccaagatcg catacatcgg tgcgaatcac
     8581 aacagcacag taagaaaaat ctccaacatc tttattccaa tcccgatcac taaagcaatc
     8641 cagcatgaaa ctgtggttgc atcgcaacaa cccatgacca gtctttttga acaggtgcta
     8701 ttgttatttg gcgatgcgat aactttaatg cttatggaaa gaatggggtt acaagaaagc
     8761 gatattctcc gtaaacatgc caacctggaa taatcaatgg cactgtttga catccatcct
     8821 ggataatact caatgatgga gtgcctgaca atgccagccg caacgctagt gagaatctaa
     8881 aaaaccattg aacaagtgtt cctggttata agaccatcct tctttttcaa accagaaaaa
     8941 tagataaaaa agagaagggg attcttaatt cttgaagaat tgacgcgcca gccttacgat
     9001 ccccctgtgg gaatcgtaag gtcttttcct tgagcctgat gatcaagcgt tttgatgaga
     9061 tggattccct gcccaccaaa gacaaagggg gtatctcacc ttccattgcg catgtcatgt
     9121 tgagcgaagc gaaacatctc accatttgta agttg
//
LOCUS       DAOSDS010000083         9112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2835_length_9112_cov_4.577896, whole genome shotgun sequence.
ACCESSION   DAOSDS010000083 DAOSDS010000000
VERSION     DAOSDS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9112
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2835_length_9112_cov_4.577896"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLL88_08970"
     CDS             <1..412
                     /locus_tag="PLL88_08970"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOJ01737.1"
                     /translation="LKPLLAEAKDDAIGKVAIGTVKGDLHDIGKNLVAIMMEGAGLEV
                     LDMGVDVPAEQFIEIIEKEQVDIIAMSALLTTTMANMEITMRMIAERGLRNRVKIMVG
                     GAPLSKEYAESIGADGFAADANQAAKMAIQLMAK"
     gene            498..1646
                     /locus_tag="PLL88_08975"
     CDS             498..1646
                     /locus_tag="PLL88_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOJ01738.1"
                     /translation="MESLSSRKRVALAIHHQEPDRVPISLGGSANHLTENLYRLLKQH
                     FKFEDNGRRTLVGFYSTPDYTPLLECLGTDMRFLHLRPVKSFIMNPVAPNFKPFVDEW
                     GLRHELLSGFYNLGGEPLGYLETIADIEKYAWPDPYLPERVGGLKAEAEQLYHFTEYS
                     LVGHRPVYGNAWEMARFLLGMEKALFMTAKDRALFEAFFDKLSDVLDGFYEVFLNEVG
                     PYIQIVEMAEDLGTNNGPMINPKFYREVMWSYHRRTIERIKKKAPHVKVMLHCDGAIR
                     RFIPDLIEAGFDILNPIESHLIGMEPAGLKKDFGMDLVFMGGVDLKNVLTKGTRSDIQ
                     KEIRTRIEEMGKGGGYILAPSHNFTNDVPLENLLTFFEEGKKLGVYPL"
     gene            1661..2323
                     /locus_tag="PLL88_08980"
     CDS             1661..2323
                     /locus_tag="PLL88_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ01739.1"
                     /translation="MRLEELRNDVLKTARDLAKYGLVWMAGGTACARDVHSGLVAVTP
                     SGLDYADLHAEDVVLMDLQGEVIEGHHKPSCASELWLRFFRKRKDLNAIVHTHSCYAT
                     AFSVVNQPIPIITETQADWFGEPIPVAPYAHVEDDAFLHDPVEALGNGYGVLLGRHGV
                     LTFGKNLRDALERALTLEEAAKTFIMAKTIGTPEIFSVEEARRSFDFYHTRYGQPGRK
                     GT"
     gene            2324..2782
                     /locus_tag="PLL88_08985"
     CDS             2324..2782
                     /locus_tag="PLL88_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ01740.1"
                     /translation="MFRIDEDFVLLDVEATSADELIRFMCKELEAAGCIQSEHVDAVL
                     EREQKFPTGLPTKPFCVALPHGEPHGVNESALCFARLKKPVVFKNMGNACEDLDVRMV
                     FLLVNNSSENQVEALRNLSEMFGESEKLSTLALLENKKDVAKMLSNTILK"
     gene            2836..3132
                     /locus_tag="PLL88_08990"
     CDS             2836..3132
                     /locus_tag="PLL88_08990"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01741.1"
                     /translation="MGYRIVVACGAGINTSTLGEDMIRERLSKEGREDIEVIHVLMSN
                     IEAYEDNMDILVSMQQVYREFKCPVVSGLPFLIGTKSEKEALLDKIMELVKNLK"
     gene            3179..4456
                     /locus_tag="PLL88_08995"
     CDS             3179..4456
                     /locus_tag="PLL88_08995"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HOJ01742.1"
                     /translation="MEVIKTAVSWFLGLGSTVIVPIVLMILGFAFKVEAKKTIRGAIM
                     TGVGLAGLFLIVDLIVAALQPAVAAMATRMGLSLTLVDVNWADAGIAWGWPGVAGVLL
                     ITLAVNAVLISLRITKTLWVDIWSYWHGSALAGFVWALTGNLWWGIVAGVLYLTIGGF
                     LGDWTAKDIQEFHGMPGISVPCGTTVQAGVFAKLITPILDKIPGFKNWDASPDAIRKR
                     FGLLGEPVVMGAILGIVIGIAAGYNVQKILGLAMQVATMMVVLPRMVSIISEGLIPIA
                     QGVIGFMREKFGNREIYVAVDCAVTLGHPSVMASTVILYIIAILLAAVLPGNKLLPIA
                     SLAVIPFWVGSVVPYTKGNVVKTVLVMALYMIPVLYIASVVAPVQTQAYNMMGQYTEE
                     IAKGTLLGSFDMGGDPLGFLIQNVMRLLGLSVL"
     gene            4614..4952
                     /locus_tag="PLL88_09000"
     CDS             4614..4952
                     /locus_tag="PLL88_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01743.1"
                     /translation="MSSFHVCVLGGSCGNRMVIIADHLTELFRQKGYNCTVTSKSVWE
                     CFTFPPAADLLLQLLPAFSDEEMEYPSINIRPLLQNINDPGTLERIFSVIEREGSVSA
                     IPAIEETGKV"
     gene            4953..6086
                     /locus_tag="PLL88_09005"
     CDS             4953..6086
                     /locus_tag="PLL88_09005"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOJ01744.1"
                     /translation="MYFDYCATTPLSDEVLEAMRPFWQENFGNPSSLHMEGKIAARAL
                     EHARCQVAELLHTDKEEIVFTSGATEANNLALLGVLRPWEDKKAHLITSAVEHHSVLH
                     IAQACERMGCAVTYLPVDAKGLISPHELRRAIRKETRLISIMHVNNEIGSVQPIEEIG
                     KIAAENNLYFHCDAVQGMGLLEVMVKDVGIHLLSISAHKMYGPKGTGALYVQKGIPIQ
                     SLMYGGSQEGRIRPGTENVPGIVGLGKTAELASRHRHENRLYIGQLRDRLIRSLRQAC
                     PNLLINGADGDRVCPHVVSLTFPGSIAEMMQIKLAMQGVAVSLGSACNSREIEPSHVL
                     LAIGLKRESADATIRVSIGNKTTIEEIIRLSEILPSVASACRI"
     gene            6124..7098
                     /locus_tag="PLL88_09010"
     CDS             6124..7098
                     /locus_tag="PLL88_09010"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOJ01745.1"
                     /translation="MRVKLDENRDDLLAYVAELYYDLKWNQEDIAKEVGVTRSMVSRM
                     LSEAREKKFVEITVHRPLSLDIELMDRFKSLFDIEDVQIVHQSFPNDLRQRERVGWAA
                     ASLAEKLLAPHTVLGLVWGTTVACFVNRLVRSNLKHLEIDVVQLVGAIASRDFAYSGL
                     ELVRAAAHALGGHPYYLNSPFYLENSEMVANLLKNKIVDETFQMMEKCRYAVVGIGSL
                     APELASFYLSGDISSEELAIIRKTGAIGSVCGLHFDIRGEQVAQFCSERTVTIQRTQL
                     DQIPVRVGMACGVGKTEPILGALRGKFVTHLVTDSITARQVLELAYKS"
     gene            complement(7095..7904)
                     /locus_tag="PLL88_09015"
     CDS             complement(7095..7904)
                     /locus_tag="PLL88_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOJ01746.1"
                     /translation="MRREIFFIFFFLLSFCCNCSPSLSRQQYAFQFSTDEIQTITIEA
                     QKMLITVIPSPDTWLHVSYDDFLEEDEKRIAVSTENHALIISGQRNAQTENGIIISMP
                     SGIEVEINAFHADFSLQNLSGNISVRTVDGDMVAENMQGIILLRTARGNITISDSQGD
                     ISVLGEHGVLTLDSLHGDIDSSTVVGSIQFSGQVLADDDIFLESDHGSVTATLTDSEN
                     AFINLWSANGTVTCMLTGVEMTNITCQREPEVRVGSFQIKTVWGRIIVNSD"
     gene            8073..>9112
                     /locus_tag="PLL88_09020"
     CDS             8073..>9112
                     /locus_tag="PLL88_09020"
                     /inference="COORDINATES: protein motif:HMM:NF023598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HOJ01747.1"
                     /translation="MAKKYDQLAIDNLNSFVFGKSPFPVRLNNGFTIGGGEVYPEINF
                     TLPGMAINTETFSEVRNQYAQMINDVVKRAVELHAPGFSVEFELLPDLTLNPQWGAEI
                     TKILREKLDEVEQKENIKTCLRVTPNDIREFARPPLMRHGKYVDEMFRCFELCAEAGA
                     DLLAIESTGGKEIHDEAIIHGDLLASVFALGVLGSRDMNDLWQKIVAIADAHKIIPSG
                     DSACGFANTAMVLAEQHFIPRIWAALIRVMSVPRSLVAFEQGAVGPNKDCAYEGVYIK
                     AITGAPIALEGAEAACAHLSQIGNIAKAVPDLWSNESVQNVKLLSASAPTVSMEQLVY
                     ATRLMNKASAKG"
BASE COUNT         2408 a         1914 c         2460 g         2330 t
ORIGIN      
        1 attaaaacca ttattggcgg aagcaaagga tgatgcaatt ggcaaggtcg ctatcggaac
       61 agtgaaaggc gatctgcacg atatcgggaa gaatcttgtt gctatcatga tggagggggc
      121 tggattggaa gtgctggata tgggggttga tgttccagcg gaacaattta ttgaaataat
      181 tgagaaagaa caggtggaca tcattgccat gtctgccctg ttgacgacca ccatggcgaa
      241 tatggaaatt accatgcgca tgattgccga aaggggtctc cgcaatcgtg tgaagatcat
      301 ggtgggaggc gctcctttat ccaaagaata tgcggagagc attggcgcgg acggttttgc
      361 tgcagatgca aatcaggcgg caaagatggc cattcagttg atggcaaaat gaacgaaaac
      421 cagaatatgt atcggaaacc tgagaatcga tcaacgggga gtggttcgag aaaaaattag
      481 aaatcggagt ggtccttttg gaaagcctat catcacgaaa aagagttgca cttgccattc
      541 atcatcagga accagatcgg gttcctatct ctctgggcgg ttctgccaat catcttacag
      601 aaaatttata tcgcttgctc aaacaacatt ttaagtttga agataatgga cgccgcaccc
      661 tggtaggttt ttattcaact cctgattaca ccccactgct ggaatgtctg ggtacggata
      721 tgcgcttttt gcatctgcgt ccggtgaaat cgttcatcat gaatcctgtc gccccaaact
      781 ttaaaccatt cgtcgatgaa tgggggttga ggcatgaatt gctgtccggt ttttacaacc
      841 tggggggaga accgctcggc tatctggaaa ccatcgctga tattgaaaaa tatgcatggc
      901 ccgatcctta tctgccggaa cgagtaggag gattgaaagc tgaggcagag cagctatatc
      961 atttcaccga atattccctg gtggggcaca gacctgtata tgggaatgcc tgggaaatgg
     1021 cgcgctttct gctgggaatg gaaaaagcgt tgttcatgac cgctaaagat agggctttgt
     1081 tcgaggcttt ctttgataag ttgagtgatg tgctggatgg gttctatgaa gtcttcctaa
     1141 acgaagttgg accgtatatc cagatcgtcg agatggcaga agatttggga accaataatg
     1201 gaccgatgat caaccccaag ttctatagag aagtgatgtg gtcataccat cgcagaacga
     1261 tcgagcgcat aaaaaagaaa gccccgcatg tgaaagtcat gctgcattgt gatggagcga
     1321 tccggcgttt cattccagac ctgatcgaag ccgggttcga cattctcaac cctatcgaat
     1381 cgcatctgat tggcatggag ccggcgggtt tgaagaagga ttttggcatg gatctggtat
     1441 ttatgggtgg ggtcgacctg aaaaatgtct tgacaaaggg cacccgcagc gatatccaaa
     1501 aggagattcg cacacgcatt gaggaaatgg gaaagggagg cggctatata ttggcgcctt
     1561 cgcataattt caccaatgat gtgccgctgg aaaacctgtt gacatttttt gaagaaggaa
     1621 aaaaactggg agtgtatccg ctctaaaggt gtgaggaacg atgaggctgg aagaacttcg
     1681 caatgacgtg ctcaaaaccg ctcgcgattt agcgaaatat ggattggtgt ggatggcagg
     1741 gggtacagct tgtgctcgcg acgtacacag tggattggtg gcggttacac ccagtgggct
     1801 ggattatgct gatctgcatg ctgaagatgt cgtcctgatg gatttgcagg gtgaggtgat
     1861 tgaagggcac cacaaacctt cgtgtgcaag cgaattatgg ttgcgatttt ttcgcaaacg
     1921 gaaagatctg aatgccatcg tccataccca ttcctgttat gctacagcgt tttcggtcgt
     1981 caaccaaccg attcctatca ttaccgaaac gcaagcagac tggtttggag aacccattcc
     2041 cgtggcaccc tatgcgcatg tagaagatga tgcgttcctg catgatccgg tagaggcatt
     2101 gggaaacggc tacggggttc tgcttggaag acacggcgtg ttgactttcg gaaaaaacct
     2161 ccgtgatgct ctggagcgcg ctttgacttt ggaagaagca gccaagacct tcatcatggc
     2221 gaaaacgatc ggtacgccgg agattttctc ggtagaagaa gccaggcggt cgttcgactt
     2281 ttatcatacc cgctatgggc agccaggtag gaaaggaaca tagatgttca ggattgatga
     2341 agatttcgtc ctgcttgacg tagaggcgac gagtgctgat gaattgatca ggttcatgtg
     2401 taaggaactt gaagcggctg gctgtattca atctgagcat gtcgacgcag tattggaacg
     2461 ggaacagaaa tttcccaccg gtcttccaac caagccgttt tgtgtagcct tgccgcatgg
     2521 agagccccac ggggtcaatg aatcagcatt atgttttgcc agattgaaaa aacctgttgt
     2581 gtttaagaat atgggcaatg cgtgtgaaga cctggatgtg cgcatggttt ttcttctggt
     2641 gaacaattcc tctgaaaatc aggttgaggc tttgcgaaac ctgtctgaaa tgttcggtga
     2701 atcggaaaaa ttatcaacat tggcattgct ggaaaataaa aaagacgttg ccaaaatgtt
     2761 gtcaaacacg atacttaagt agcaaaggag gttcaaggaa aactaaacag caaacaatga
     2821 aaggagaaaa caaaattggg ttacagaatt gttgtcgctt gcggcgcagg aattaatacc
     2881 tccactctgg gtgaggatat gatccgtgag cggctaagca aggaaggacg cgaggatatc
     2941 gaggttattc acgttctcat gtccaatatc gaagcatatg aagacaatat ggatattttg
     3001 gtttccatgc agcaggttta tagagaattc aagtgcccgg tagtgagtgg cttgcctttt
     3061 ttgatcggga caaaaagcga aaaagaagca ttactagaca aaattatgga attggtaaaa
     3121 aacctgaaat aacaggctgg aaaacgaaag aatcctaaca taaaaaagga gattatcaat
     3181 ggaagtcata aaaacagcag tttcatggtt tttaggctta ggttccacag tgatcgtacc
     3241 gattgtattg atgatccttg ggttcgcctt taaggtagaa gcgaagaaaa cgatccgtgg
     3301 tgcaatcatg actggtgtag gtctggcagg tctgttcctc attgttgatc tgatcgttgc
     3361 tgcattacaa cccgctgttg cagcaatggc cactcgcatg ggtttgagct tgaccctggt
     3421 agatgtaaac tgggctgatg ccggcatagc gtggggatgg cccggcgttg caggcgtgtt
     3481 attgatcaca ttggctgtga atgcagtgct gatctctctc cggattacaa aaaccctatg
     3541 ggtagatatc tggtcgtact ggcatggttc cgccctggcg gggtttgtgt gggcgttgac
     3601 tggaaatctg tggtggggta ttgtggctgg agttctgtat ctcaccatag gcggttttct
     3661 gggtgattgg actgcgaaag atatccagga atttcatgga atgcccggaa tcagcgttcc
     3721 ttgcggaacc acggtgcagg ctggcgtgtt tgccaagttg atcactccca tcctggataa
     3781 gatcccaggt ttcaagaatt gggatgcatc tcccgatgcg atccgcaagc gctttggttt
     3841 gctgggtgag ccggtggtta tgggtgcaat tctaggtatc gtgatcggca ttgctgccgg
     3901 atataatgtg cagaagatct taggactggc gatgcaggtg gcgaccatga tggtggtctt
     3961 gccgcgcatg gtttccatca tttccgaagg tcttatcccg attgcgcagg gcgtcattgg
     4021 tttcatgcgt gaaaaattcg gcaatcggga aatctacgtt gcagtggatt gtgcggtaac
     4081 cctggggcat ccttctgtga tggcatcaac cgtgatcctg tacatcatcg ctattctttt
     4141 ggcagcggtt cttcccggaa ataaactgct gccgatcgct tcactggcag tgatcccatt
     4201 ctgggttgga tcggtggtgc cctacaccaa aggcaatgtg gtcaagaccg tgctggttat
     4261 ggcgctgtac atgatcccgg tcctttacat tgccagcgtc gttgctccgg tccaaacgca
     4321 ggcatataac atgatgggtc aatataccga agagatcgcc aaaggaacgt tattgggctc
     4381 attcgacatg ggcggcgatc cactgggttt cctgatccag aatgtgatgc gcttattagg
     4441 tttgagcgta ttgtaaaaag atcagcttgc atggaaatgc aagcgtgatc gcagcaagtt
     4501 gggagagcgg ttcgtaccga ctcccaactt gcaggggttt cggatacaac caccgtcacg
     4561 aaaagatacg ataggaattt cttgatggga aatcgaatga ttcagggatc actatgtcca
     4621 gttttcatgt atgtgtgttg ggagggtctt gtggcaaccg catggtcatc attgccgatc
     4681 atcttaccga actgttcagg caaaaaggct acaattgcac tgtaacctcg aagagcgttt
     4741 gggaatgttt cacgtttccg cccgcagcgg atcttttatt gcagcttctc cccgctttta
     4801 gcgatgaaga aatggaatac ccctccatca atatccgccc gctgttgcag aacatcaacg
     4861 atcctggtac attggaaagg attttctcgg taattgaaag ggaaggcagc gtgtctgcaa
     4921 ttccagctat tgaggaaaca ggaaaagtct agatgtattt tgattactgt gcaacaacgc
     4981 cgctatccga tgaagtattg gaagccatgc gaccgttctg gcaggagaat tttgggaatc
     5041 cttccagcct gcatatggaa ggaaagattg ctgcccgggc attagagcat gcccgttgcc
     5101 aggtagctga actgttgcat acagataaag aggaaatcgt ttttaccagt ggagcgactg
     5161 aagcgaacaa cctggctttg ttgggggtgt tgcgtccctg ggaagataaa aaagctcacc
     5221 tcatcacaag cgcagtggaa catcattctg ttttgcacat agcccaagca tgtgaaagaa
     5281 tgggctgcgc ggtcacttat ttaccagtgg atgcgaaagg gttgatctct ccgcatgaac
     5341 tgaggcgggc aattcggaaa gagacgcgtt tgatctccat catgcacgtg aacaacgaga
     5401 tcggcagcgt tcagccaata gaggaaatcg gaaagatagc cgcagaaaat aatctttact
     5461 ttcattgcga cgcagttcag gggatggggt tgctggaggt gatggtgaaa gatgtgggta
     5521 ttcatcttct ctcaatctct gcacacaaaa tgtatggtcc taagggcacg ggggcgcttt
     5581 acgtccaaaa gggtattccc attcaaagtt tgatgtatgg gggatcacaa gaaggaagga
     5641 tccgtccggg aacagaaaat gtgccgggta ttgtaggttt ggggaaaact gctgaactgg
     5701 cgagcaggca tcggcatgaa aacaggcttt acatcgggca attaagagat cgtttgatcc
     5761 gatctttaag gcaagcctgc cccaaccttc ttatcaacgg ggctgatgga gatcgggttt
     5821 gcccgcacgt ggtttcgctc acctttccag gatcaatagc tgaaatgatg caaataaaac
     5881 ttgccatgca aggtgttgcc gtttctctcg gatcagcctg taattcaaga gaaatcgagc
     5941 cgtctcacgt tcttctggcg attggattga agcgagagag tgccgatgcc accatcaggg
     6001 tttcgatcgg aaataaaacc acgatagagg agatcatacg attatccgaa attctcccca
     6061 gcgttgcttc tgcatgtaga atataattgg ttcgaaaatt ctatcgatca ggattgatga
     6121 agcatgcgcg taaagctgga tgaaaatcga gatgatcttc tggcatatgt tgccgaactt
     6181 tattatgatc taaagtggaa ccaggaggac atcgcgaaag aagtgggggt aacgcgatcg
     6241 atggtttcgc gtatgctcag cgaggcgcgt gaaaaaaagt tcgtagaaat cactgtgcac
     6301 cgaccgcttt ctttggatat agagctgatg gatcgattta aatcgctgtt tgatatcgaa
     6361 gatgttcaga tcgtccacca gtcttttccc aatgatctac gccaacgaga acgggtggga
     6421 tgggcagccg cctccctcgc ggaaaaattg ctcgcacctc acaccgtgct gggattggtg
     6481 tggggcacca ccgtagcctg ttttgtgaat agacttgtca ggagcaacct gaaacacctg
     6541 gaaattgatg tggtgcagtt ggtgggcgcg atagcgtcaa gagatttcgc atacagcggt
     6601 ctggaactgg taagagctgc cgcacatgct ctgggcggac atccttatta tctcaattcc
     6661 cccttctatc ttgaaaattc cgaaatggtt gccaatcttt tgaaaaacaa gatcgtggat
     6721 gagaccttcc agatgatgga aaaatgccgg tatgccgtgg tcggcattgg tagtcttgca
     6781 ccggaattag ccagctttta tctttccgga gatatctcat cagaagaatt ggctatcatt
     6841 cgaaagacgg gagccatagg cagcgtctgc ggattgcatt tcgatatccg cggcgaacag
     6901 gtggcacaat tctgttcgga acgaacagta acaatccaac gaacgcagct tgaccagatt
     6961 cctgttcggg tgggcatggc ttgcggggtt ggaaaaacag aaccaatcct tggagcgttg
     7021 cgcggaaaat tcgtcaccca tctggtcaca gatagcataa ctgccagaca ggttctggaa
     7081 ttagcctata aaagctaatc ggaattcacg atgatgcgtc cccaaactgt ttttatttga
     7141 aaggagccaa cacgcacctc tggttcacgt tggcaggtga tgttggtcat ctccacccct
     7201 gttaacatac aggttaccgt tccattcgcg ctccacaggt taataaaagc gttctcagaa
     7261 tctgtcaggg tggctgttac gctgccatga tctgactcta gaaagatgtc atcgtcagcc
     7321 agcacctgcc ccgagaattg aatagatccc accactgtgg aagaatcgat atcaccgtgt
     7381 aaagaatcaa gggtcagcac accgtgttca cctaaaaccg agatatctcc ctgcgaatca
     7441 gatattgtga tatttcctcg cgcagttcgg agcaggatta tcccttgcat gttctctgca
     7501 accatatcgc catcaacggt tcttacactg atatttccgg ataaattttg caaggaaaag
     7561 tctgcatgaa aggcgttgat ctctacctca atcccgctcg gcatcgaaat gatgatgcca
     7621 ttctctgtct gtgcgtttct ttgacctgaa ataatgagcg catggttttc tgtggaaaca
     7681 gcgatacgtt tttcatcctc ctccagaaaa tcgtcgtaag agacgtggag ccaggtgtcg
     7741 ggagaaggga tgaccgtgat gagcattttt tgcgcttcga tggtgatggt ctggatttca
     7801 tcggtggaaa attgaaaagc atactgctgg cgagaaaggc taggagaaca gttgcaacag
     7861 aagctcaaga ggaaaaagaa aatgaaaaag atttctcggc gcatagactc tcaacaggca
     7921 gattcgttaa cggtcctgtt agtttagcat tgattcattg tttggaattg gggaggttaa
     7981 aatgctttca gccacaaaaa atgaccagga attgttgaca acgttgtcat agtttgatat
     8041 agtatggatg aattctgtgc gtgcgaggtc ttatggcaaa aaaatacgat caattggcga
     8101 tagacaacct aaattccttt gtttttggta aaagtccatt tcccgttcgg cttaacaatg
     8161 gcttcaccat cgggggtgga gaagtgtacc cagagatcaa ctttacctta cctggcatgg
     8221 cgatcaatac agaaaccttt tctgaggtga ggaatcagta tgcgcaaatg atcaacgatg
     8281 ttgtcaaaag ggctgtagaa ttgcatgcgc ctggtttcag cgtggaattt gaactgttgc
     8341 ctgatctgac gttgaatccg caatggggag ccgaaattac caaaatcttg cgggaaaagc
     8401 tggacgaggt tgaacaaaaa gagaacataa aaacctgttt gcgcgtaact cccaacgata
     8461 ttcgcgaatt tgcgcgaccg cccttgatga ggcatgggaa gtacgtagat gagatgtttc
     8521 gctgtttcga gctttgtgca gaggcgggtg cggacctgct ggctatagag tctacaggag
     8581 gaaaagagat ccatgatgaa gcgatcatcc atggcgatct gctcgcatct gttttcgctc
     8641 tgggcgttct gggcagccgc gatatgaatg acctctggca aaagattgtc gctatcgcag
     8701 atgctcacaa gatcatcccg tcaggtgatt cggcgtgtgg atttgccaat accgccatgg
     8761 ttttggctga acaacatttc ataccgcgca tctgggcagc tttgatccgc gtgatgagcg
     8821 ttccccgcag cctagtcgcc tttgaacagg gcgcagttgg tccaaacaag gattgtgcat
     8881 acgaaggggt gtatatcaaa gcgatcacgg gcgctcccat tgcactggag ggtgctgaag
     8941 ctgcctgtgc gcatctgtct caaatcggaa atatcgccaa agctgtaccg gatctgtgga
     9001 gcaacgaatc ggtgcagaat gtaaagctgc tgtcagcaag cgctcccacg gtttcgatgg
     9061 agcaactggt ttatgcaaca cggcttatga acaaggcgtc tgcaaaggga ca
//
LOCUS       DAOSDS010000084         8986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2876_length_8986_cov_6.437465, whole genome shotgun sequence.
ACCESSION   DAOSDS010000084 DAOSDS010000000
VERSION     DAOSDS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8986
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2876_length_8986_cov_6.437465"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..793
                     /locus_tag="PLL88_09025"
     CDS             14..793
                     /locus_tag="PLL88_09025"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOJ01748.1"
                     /translation="MTAFIKSETTRAGFQRAVVGVSGGIDSALACVLAAKALGPQNVL
                     AVRMPYRTSTADSLEHAQLIIDQFGVQSTTVPISDMADGLIAKFPDMNEQRKGNIMAR
                     CRMIVLYDQSSALNALVVGTSNKSEILLGYSTQYGDSASAFNPIGDLYKTQVRQLSAA
                     LAIPEVIINKAPSADLWANQTDEGELGFTYAEVDKLLYLLVDERYTPEECVEAGFSKS
                     FVDTVVNRIRRYHYKRMPPPIAKLGNRTIGYDFLYLRDWGT"
     gene            796..1386
                     /locus_tag="PLL88_09030"
     CDS             796..1386
                     /locus_tag="PLL88_09030"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOJ01749.1"
                     /translation="MFLRKIALSLFLFTLCSACAPNLPAPTVTPVPASTSTAIPTETP
                     SPTVAPTAEPTSATSKPQTILIYLIAVGDQGASGELIGCGDSLVPVEVAIEPTVAVLR
                     AALTALLNLAPDFTYGQSGLYNALDLSALKIDALDIVDRQAIIHLSGSLVSGGECDIP
                     RIEAQLTATALQFSTIDSVTIYVNDILLQDLLDLRG"
     gene            complement(1397..2458)
                     /locus_tag="PLL88_09035"
     CDS             complement(1397..2458)
                     /locus_tag="PLL88_09035"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01750.1"
                     /translation="MKKKKVALLANLKVNAPHWEGMPEDQWDDLDSEKTINTILGAMH
                     NGGYECEFFEGNFKIIETLPKYKPDICFNICEGYYGDSREAHIPAILEMLRIPYTGSR
                     VLTLALALDKPMTKRILDFHELPTPPFQTFERVDEPLDPTLRFPLFVKPSREGTSMGV
                     SLKSVVNTKEELYEQVGHIMHRYHQPALVEEYIEGREVTIGMIGNLHGPIARRIPHDV
                     NLPRIQSGLRFLPPMEVDLKPYLLTDAVYSNHLKVALAEDLNYICPAPLDEDMVDELN
                     WLAAAVFRVLGTLDVARVDFRLDVKDNWKPYILEINPLPGLSSVSDLVIAGRADEVSH
                     DALIVMVLETALERHNLLK"
     gene            complement(2495..4378)
                     /locus_tag="PLL88_09040"
     CDS             complement(2495..4378)
                     /locus_tag="PLL88_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOJ01751.1"
                     /translation="MSLITAAHLAKSFGAEDLFSEISLSIPQHARIGLVGENGIGKTT
                     LLRMLYGIDKPDEGTIARARDLKMGYLPQESRFQSSQTLQQECRDALADVFNLQKEID
                     ALTAQLEKDPNDDALINRLGDLHERYERLGGYTIDSYILRVISGLGFEAADLTRPISQ
                     FSGGERTRAYLAKLLLMNPTLLLLDEPTNHLDIQAMEWLEDFLKKYSGAVLIVSHDRY
                     FLDQTVNTIWEMTPAIESYPGNYSAFLKLRQERMERQMKEYESQQEYIAKEEDYIQRN
                     IAGQNTAQAKGRRRRLERLLKEGQLIPPARKRRMLFGFKDNRRSGDIILRTKGVQIGF
                     KDEAVSLFRMPDVELRRGECVGVIGPNGAGKTTLLRTILGQIPPLQGVISLGASLDVG
                     YFAQAHEDLHPDWTLMQEIQANAPKMLPADMRNYLGKFLFSGDDVFKTVNVLSGGERG
                     RLALAILALKGVNLIFLDEPTNHLDLPSQEILQAVLADFDGTILLVTHDRYLLEALAT
                     QIWMVDAVSKTLQVFQGGYTEFMAVRRMPAEPPVKEHPATAQPMPRKVPAKKLNKQKL
                     HELEVRIHETEIEIHRIEQELKDPDLEAEEVGELGETYLFLQEQLDDMINQWDDYYEK
                     SNT"
     gene            4535..5416
                     /locus_tag="PLL88_09045"
     CDS             4535..5416
                     /locus_tag="PLL88_09045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ01752.1"
                     /translation="MAKPPICDYEGSDYQKSFWEEGGRAYEDAAEAIALRRLLPKSGS
                     HLLELGAGAGRNTPRYAGFQKITLLDYSRTQLEQARARLGDSPRYRYVAADACNLPFA
                     SAVFDAATMIRTIHHLEEGESALKQVSQVLAKQAVFILEFANKRNLKAILRYLLGRQK
                     WSPYTLEQVEFVELNFNFHPRQIRRWLRQCGFFVERQLTVSHFRVGFLKRHVPLGILS
                     SLDAALQWTGAFWQLTPSVFVRSTHTQNAQPLQDFAFRCPQCGSHPLKAIQADIQCPQ
                     CGRIYPYQDGIYDFRVK"
     gene            5462..5929
                     /locus_tag="PLL88_09050"
     CDS             5462..5929
                     /locus_tag="PLL88_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01753.1"
                     /translation="MQLITTCSHCGSSFKWDDAFWQGLGMPDCPACGFNNQTGKVGKK
                     EGPIERGYHKLAQQYHDAYGALQSVEYLSHFPKFEINFNFEKATVYSGSRQGRYDVNF
                     LSLGYIGEGPRYAKAFLDELGFSLTSEEIDGIKPGAVMTLEEGKVVIKYKENS"
     gene            complement(6825..7571)
                     /locus_tag="PLL88_09055"
     CDS             complement(6825..7571)
                     /locus_tag="PLL88_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01754.1"
                     /translation="MVVNDSILLPKEWFAVQYERAHEQFTKSGFRTYMEIKFSLQNRK
                     KHFDKQKLEEAVEKSQIYTFGWPIGIILESNEQFRPKPSENGIYLEGKTKSENIYKGT
                     YDYWALNCNGDFYQLKSIYEDTRNTIAIFFNTRIVRVTETLLFCEKLYSNLGLADENP
                     WVDIEINYFGLKGRLLRSSNFTRHIEKRFTDENEVRTKGTVSIDEISPKIIELTEQFA
                     WPLFVVYEHCGIDYEIYEDIVTDFIHGKVT"
     gene            complement(8283..>8986)
                     /locus_tag="PLL88_09060"
     CDS             complement(8283..>8986)
                     /locus_tag="PLL88_09060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ01755.1"
                     /translation="RGEPSYVWRAGQERRLKMICQYAGERAQGVILENGCGVGEYLQR
                     LDEGAKLAVGLDVDIERVLETHHKGLTVQLAVGEALPHATGTFDLVISNEVIEHVQDD
                     RQALTEMVRVLKPAGRIILFCPNRGYPFETHGIYRKGEYHFGNKLFVNYLPDRWRNKL
                     APHVRVYRRRDLDKLFAGLPVRFVYRTVIFGGYDNIIRRSPTLGGLLRGLLQFLEKTP
                     LRVLGLSHFWVVEKR"
BASE COUNT         2212 a         2409 c         2134 g         2231 t
ORIGIN      
        1 ggcaaccaac attttgaccg ctttcatcaa aagtgaaacc acccgcgctg gctttcaaag
       61 agcggtggtg ggagtttctg gcggcatcga ttcagccctt gcctgtgtgc tggcagccaa
      121 ggcgctcggt ccgcagaatg tgctggcggt gcgcatgccc taccgcactt ctactgccga
      181 ctcgctggaa cacgctcaat tgatcatcga ccagttcggc gtgcagagca ccaccgttcc
      241 catcagcgac atggcagatg gcttgatcgc gaaatttccc gatatgaacg agcagcgcaa
      301 aggcaatatc atggcgcgct gccgcatgat cgtgttgtat gaccaatcca gcgcgttgaa
      361 cgcgctggtg gtgggcacca gcaacaaaag cgagatcttg ctgggctatt ccacccagta
      421 cggtgattca gccagcgctt tcaaccccat cggcgatctc tataaaactc aggtgcgcca
      481 actatcggcg gctctggcaa tccccgaagt gatcatcaac aaagctccca gcgccgatct
      541 gtgggcaaac caaacggacg aaggggagtt gggtttcacc tatgccgaag tggataagct
      601 gctctatctg ttggtggatg aacgctacac ccccgaagaa tgcgtggaag ccggcttttc
      661 caagtcattc gtggatactg tggtgaaccg catccgccgc tatcattaca aacgcatgcc
      721 accacccatc gccaaattgg gcaaccgcac catcggctat gattttttgt acctgcgaga
      781 ttggggaacc tagctttgtt cctgcgcaag attgcccttt ccctttttct atttaccttg
      841 tgcagcgcct gtgcacccaa cctgccagcc ccaaccgtca cgcccgttcc tgcatctacc
      901 agcacagcca ttcccacaga aacgccctcc cccactgttg cgcccacggc tgaacccacc
      961 agcgccacat ccaaaccaca aaccatattg atctacctga tagcggtggg agatcagggt
     1021 gcttccggag agctgattgg ctgcggcgac agcctggtgc cggtcgaggt agccattgaa
     1081 ccgaccgtag cagtgttgcg cgctgccctt accgccctgc tcaacctggc acccgatttc
     1141 acttacgggc aatctggctt atataacgct ctcgatttat ccgctctcaa aatcgatgcg
     1201 ctagacatcg tggatcggca agcgatcatc catttaagcg gttcactggt aagcggcgga
     1261 gaatgcgata tcccccgcat cgaagcccaa cttacagcta cagcattgca gttctcgacc
     1321 attgactccg ttactatcta cgtcaatgat atcttgcttc aggatttgct cgatctgcgg
     1381 gggtaatatc tcggatttat ttcaataaat tgtgtctttc cagcgcggtt tccagaacca
     1441 tcacgatcaa cgcatcgtgc gagacctcat cggcacgccc tgcaatgacc agatcagaaa
     1501 cactggataa acccggcagc ggattgatct ccaaaatata cggtttccaa ttatccttta
     1561 catccagcct gaaatctaca cgcgccacat ccagggtgcc caacacacga aagaccgcgg
     1621 ctgccaacca attgagttcg tctaccatgt cttcatccag cggcgccggg cagatgtaat
     1681 tgaggtcttc cgccaaggct actttcaagt ggttactgta taccgcgtca gtcaacagat
     1741 agggtttgag gtccacttcc ataggcggca gaaagcgcaa tccagattga atgcgcggca
     1801 ggttcacatc gtgcggaatg cgacgcgcga tgggtccgtg caagttgccg atcatcccga
     1861 tggtcacctc acgcccttcg atatactcct ccaccaaagc aggttggtga tagcggtgca
     1921 tgatgtgccc aacctgttca tagagctctt ctttggtgtt caccaccgac ttcaagctca
     1981 cgcccatgct ggtaccctcc cggctgggtt ttacgaacaa cgggaagcgc agggtggggt
     2041 ccagcggttc atccaccctt tcaaaagttt gaaaaggcgg ggtgggcagt tcatggaagt
     2101 caaggatgcg cttggtcatg ggtttgtcca gcgccagtgc cagggtgagc acgcgtgagc
     2161 cggtataggg aatgcgcaac atttccaaaa tggcaggaat atgcgcttcg cgtgaatcgc
     2221 catagtaccc ttcgcagata ttgaagcaaa tatccggctt atactttggc aaagtttcaa
     2281 tgatcttgaa atttccttca aagaattcgc actcataacc cccgttgtgc atcgctccta
     2341 aaatcgtatt aatggtcttt tcagaatcca ggtcatccca ctgatcttca ggcatgccct
     2401 cccagtgagg tgcgttgact ttcaggttcg caagcagagc aacctttttc tttttcaatg
     2461 taaataactc ctcattcagc tatagtaaaa ataatcaagt attagatttt tcataataat
     2521 catcccattg attaatcata tcatcgagct gttcttgcaa gaagaggtaa gtttctccga
     2581 gctcaccaac ttcttccgct tccaggtcgg gatcctttaa ctcctgttcg atcctgtgaa
     2641 tctcgatctc tgtttcatgt atacgtactt ccagctcgtg cagtttctgt ttattaagtt
     2701 tcttagccgg aacctttcga ggcatcggct gcgctgttgc gggatgttct tttaccgggg
     2761 gttctgccgg catccgacgt acagccatga actccgtgta cccgccctgg aacacttgca
     2821 gtgtttttga aaccgcatcc accatccaga tttgggtagc cagcgcctcc aacaggtaac
     2881 ggtcatgcgt taccaacaga atcgttccgt cgaaatcagc caacaccgcc tgcaggatct
     2941 cctgcgaagg caggtcgaga tggttggtgg gctcatccaa aaagatcaga ttcacacctt
     3001 tgagtgccag gatcgccagc gccagccgcc cgcgctctcc gccgctcaga acgttcacag
     3061 ttttaaacac gtcatcccca ctgaacagga atttgcccag ataattgcgc atatccgccg
     3121 gcaacatctt gggagcatta gcctggatct cctgcatgag cgtccagtca gggtgcaggt
     3181 cttcatgtgc ctgggcaaaa tagcccacgt ccaaactcgc ccccaggctg atcacgccct
     3241 gaagcggtgg aatctgtccc aaaatggtgc gcaacagggt ggtcttgcca gcaccgttcg
     3301 gtccgatcac gcccacgcat tccccgcgcc gcagttccac atctggcatg cggaacaggg
     3361 aaaccgcttc atctttgaaa ccgatctgca ctcctttggt gcgtaaaatg atatcgccgg
     3421 atcgccggtt atccttgaac ccgaagagca tgcgccgttt gcgggctgga gggatcaatt
     3481 gcccttcttt gagcagacgc tccaaccttc tgcgtcgccc tttcgcctgg gcagtgttct
     3541 gcccggcgat gttgcgctgg atgtaatcct cttctttggc aatatattcc tgctggcttt
     3601 catattcttt catctgtcgt tccatgcgct cctggcgcag tttcagaaag gctgaatagt
     3661 tgccggggta gctttcgata gccggagtca tctcccagat agtgttcacg gtctggtcaa
     3721 ggaaatagcg gtcatgcgaa acgatcagca ccgcgccgct gtattttttc aagaaatctt
     3781 ccagccattc catggcttga atgtccaggt ggttggtggg ttcatccagc agcagcaggg
     3841 tggggttcat gagcaacagt ttcgccaagt aggcgcgcgt gcgctcccca ccggaaaatt
     3901 gagagatagg gcgggtcaaa tcggcagctt caaaacccaa accgctgata acgcgcaaaa
     3961 tgtaggaatc aatggtgtag ccccccagcc gttcataccg ctcgtgcagg tcacccagcc
     4021 ggttgatgag ggcatcatcg ttggggtctt tttccaactg cgcggtcagg gcatcgatct
     4081 ctttctgcag gttgaagaca tccgccagtg catcgcggca ctcctgttgc agggtttgcg
     4141 aagattgaaa acgcgattct tgcggcaggt agcccatttt caagtccctg gcacgcgcga
     4201 tggtgccttc gtcgggcttg tcgatgccgt acagcatgcg cagcagggta gttttgccaa
     4261 tgccgttctc gcccaccagc ccgatgcgag cgtgctgggg aatgctcagc gagatctcgc
     4321 tgaagaggtc ttctgcgcca aacgattttg caagatgggc tgccgtgatg agggacacgc
     4381 tgttttcctt tttcgtctac cgaacagaaa gacattgtac ctgaattatg tgcgattacc
     4441 ttcgccattt attccattcc atcctgattt gacagccttt ttttcacagg gtatgcttaa
     4501 cggcagatcc tatcctgagc agcggaaaca aaaaatggca aaaccaccta tttgcgatta
     4561 cgagggttct gactatcaaa aatccttctg ggaggaaggc gggcgggcat acgaagacgc
     4621 cgcagaagcc atcgccttgc gccgcctgct gcccaagagc ggcagccacc tgctggagtt
     4681 gggcgccgga gccgggcgca acaccccgcg ctacgccggc tttcagaaga tcaccctgct
     4741 cgattattcg cgcactcaat tggagcaggc gcgcgcgcgg ctgggtgatt cccctcgcta
     4801 tcgctatgtg gctgccgacg cctgcaacct gcctttcgct tcggcagtct tcgatgctgc
     4861 caccatgatc cgcaccatcc accacctcga agaaggcgaa agcgccctca agcaggtcag
     4921 ccaggtgctg gcaaagcagg cggttttcat cctggagttt gccaacaaac gcaacctgaa
     4981 agccatcttg cgctatttgc tgggcaggca aaaatggagc ccttataccc tggagcaggt
     5041 ggaattcgtg gaattgaact tcaactttca cccccgccag atccgacgct ggttgcgcca
     5101 gtgcgggttc ttcgtcgaac gccagttgac cgtctcgcat ttcagggtgg gtttcctgaa
     5161 acgtcacgtc cctctgggca tcttgagcag cctggatgct gcgctgcaat ggacgggggc
     5221 tttctggcag ctcaccccca gcgtttttgt gcgcagcaca cacacccaaa atgcccaacc
     5281 cttgcaagat ttcgcgtttc gatgtcccca atgtggctcc catccgctca aagccattca
     5341 ggcagatata caatgcccgc aatgcggcag gatatatcca tatcaagacg gcatttatga
     5401 ttttcgcgta aaatagagat atgcgctgaa cgcgcaccca gtcgatcaaa ggaggagagc
     5461 gatgcaattg atcaccacct gttcacattg tggaagttca ttcaaatggg atgatgcttt
     5521 ttggcagggt ttaggcatgc ccgattgccc ggcgtgcggt ttcaacaatc agactggtaa
     5581 ggtagggaaa aaggaaggtc ccatcgaacg cggctaccac aagctggcgc agcagtatca
     5641 cgatgcttac ggtgcgctcc aatcggtgga atatctgagt cattttccaa aattcgagat
     5701 caatttcaat tttgagaaag ctacagtgta ctccggatca cgccagggaa gatacgatgt
     5761 caacttctta tcgctgggat acatcggcga gggtccgcgc tatgccaaag cttttttgga
     5821 tgaactgggc ttcagcctga ccagcgagga gatcgacggt atcaaaccag gtgcggtgat
     5881 gacactggaa gaggggaaag tggtcatcaa gtacaaagaa aattcataaa tcctttttta
     5941 tgtttttgtg gaagatatcg gtagtgtgtt gataacgcac ctttcttttt acaatgtcat
     6001 cccgaacgca gtgagggatc tcacccaaac gaagacgatc catgaacaga tcgcaaagag
     6061 aattttacag ttattcattt ctttgcgatc tgtcggaaaa ttaacgagga aatggagaga
     6121 ttcctcagtc gctacgctct ttcgaaatga caggagagaa caaggaatct taccccaacg
     6181 aagtttatcc acaaacaatt ttcaaaaaac ggatccatat tcctgcctgt tggagaaaat
     6241 ttgcacaaca aggggagtgg caaaagttga cagcagcgca aatgcaagaa gtgcaagtgt
     6301 ggagggattg gatcccggga acgaatgcac aaaaggttcc gtttttgaca gtttgcggga
     6361 aaggagaagg tgttgataac gcgccatttc ttactgtcct tccgagccct gcgaggaatc
     6421 tcacataaga ggtaggtaaa ccagccagtg agagggtaat ccatttcctg actcttacgt
     6481 catgcagagc gaagcgatgc atcgcaccat ttgcataagc aaccatcacg ttagttaaag
     6541 aactgccaga gtgttctcct atcccaccag caacctttag tctattgact gacctacttc
     6601 attgcaactt ttcaatgctt ctaccctaga tatggagaga tccatcggtc gctgcactcc
     6661 ctctggatga cattgacaaa agcaaagtca ttgggatttt ccgtcttatt aatattgaaa
     6721 tgaggagaag ttcaaacttc tcccctgtga aaagcgtggg ggagattgaa agggggcatc
     6781 gtttagcatg actggcaagt aaacaaatac gtactccaac acattcacgt tacttttcca
     6841 tgaatgaaat cggtcacaat atcctcatag atttcgtagt cgataccaca atgttcataa
     6901 acaacgaaca atggccacgc aaattgttct gttagttcaa tgatcttggg agaaatctca
     6961 tcaatggaaa ccgtcccttt tgtacgaact tcattttcat cggtaaacct tttttcaata
     7021 tgccgtgtaa aattggacga tcgaagcagc ctgcccttta aaccaaaata atttatttca
     7081 atatccaccc aaggattttc atctgctaaa cccaaatttg aatacagctt ttcacaaaag
     7141 agtagagtct ccgtaactct gacaattctt gtattaaaaa atatggcgat cgtatttcgc
     7201 gtatcttcat agatgctttt taattggtaa aaatccccat tgcagttcaa agcccaataa
     7261 tcatatgttc ctttatagat gttctcactt tttgttttcc cctccaaata tataccgttt
     7321 tctgatggtt ttggtctaaa ttgttcattg gattccagga tgatgccaat cggccaccca
     7381 aatgtatata tttgagattt ttcaacggct tcctctaatt tttgtttatc aaagtgtttc
     7441 tttctatttt gaagagaaaa ctttatttcc atatatgtac gaaaacctga tttcgtaaac
     7501 tgctcatgcg ctcgttcata ttgaaccgca aaccattctt ttggtaaaag tatagaatca
     7561 ttaacgacca tggcttttga tctctcctca ttgaatagaa taattttaca taattattca
     7621 atacataaat cctctttgct tagatttctc aagggatatt ttcaacgaaa aacgataaac
     7681 ttctcccctg tgcgaagcat gaaagtgaat gagagggaca tcgttcagtc agggtgggtt
     7741 cagaaacgca cctcctcttt tcgtcattcc gatcagcgcg aggaatctca ccaaaagata
     7801 aataaaccta ccagcgatag ggcaatccat ttcctgatcc ttaagtcatg cagagcgaag
     7861 cgatgcattt caccaagaaa aggtcaaacc accacgtaaa tcgaggaact gccagagtgg
     7921 cctcttaacc caccagtaat atttagtcta ttgactgatc tacttcattg cgacttttcc
     7981 attcttataa cttagacatg gagagatcca tcggtcgctg cactccctct ggatgacgtt
     8041 gaaaaaatag ataggtacac tcctaacgca ccacatcact atgaagagat taatagtatt
     8101 tttatacacc agctcttttt gtttagatct ttcaagggat aactccaacg aaaaacgaga
     8161 gccttcgctt gagttcagga tggcaggtgg gtaaatggaa tgtaaagatt cctcagtcgc
     8221 tgcgctcctt cggaatgaca gactaaataa tggtgcacta tgaacgtagc acaaattcgc
     8281 acttatctct tttccaccac ccagaaatgc gagagtccca agacccgcag cggggtcttt
     8341 tccaggaact gcagcaagcc gcgcagcagc ccgcccagcg tcgggctgcg gcgaatgatg
     8401 ttatcataac cgccaaagat gaccgtgcgg tagacgaagc gcaccggcag accggcgaac
     8461 aatttatcca ggtcgcggcg gcggtacacg cgcacatgcg gcgccagttt gttgcgccag
     8521 cgatcgggca ggtagttcac gaacagcttg ttgccgaaat gatattcacc tttgcggtaa
     8581 atgccgtgcg tttcaaaagg atagccgcgg ttggggcaga aaaggatgat gcgccctgcg
     8641 ggcttcagca cacgcaccat ctccgtcagc gcctggcggt catcctgcac atgctcaatg
     8701 acctcgttgc tgatgaccag atcgaaggtg cccgtggcat gcggcagggc ttctcccacc
     8761 gccaactgca cggtcaaacc cttgtggtgg gtttccaaca cgcgttctat atccacatcc
     8821 aagcccaccg ccagctttgc cccttcgtcc aagcgctgca aatactcgcc caccccacaa
     8881 ccattttcca ggatcacccc ttgggcgcgc tccccggcgt actggcagat catcttcaac
     8941 cggcgttcct gcccggcacg ccacacataa gaaggttccc cgcgta
//
LOCUS       DAOSDS010000085         8917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2902_length_8917_cov_4.230422, whole genome shotgun sequence.
ACCESSION   DAOSDS010000085 DAOSDS010000000
VERSION     DAOSDS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8917
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2902_length_8917_cov_4.230422"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="PLL88_09065"
     CDS             <1..544
                     /locus_tag="PLL88_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ01756.1"
                     /translation="GIIGPNGAGKSMLLRIICGLVKPSRGQVVVLGEQVGVDCDFPSR
                     TGALIDIPGFLPAYSAQRNLRLLADVSGSVDQNRIAEVLSIVGLNHKNTQPVSTYSVG
                     MRKRLGIAQAILEQPELLLLDEPTDSIDQAGWKSVYEYLIQLKEAGTTILLTSNNLDE
                     ITILCDQTFLLKDRHLAPVP"
     gene            complement(748..996)
                     /locus_tag="PLL88_09070"
     CDS             complement(748..996)
                     /locus_tag="PLL88_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HOJ01757.1"
                     /translation="MNTITVKFFSHLKEKAGVASAALEIAPGATIADLKEQVTLQYPA
                     LETHLRNILILIDNRIAIDEDAIPGGCTVSFMTPIGGG"
     gene            1056..1691
                     /gene="udk"
                     /locus_tag="PLL88_09075"
     CDS             1056..1691
                     /gene="udk"
                     /locus_tag="PLL88_09075"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOJ01758.1"
                     /translation="MQIDYVPIVIGIAGGTGSGKTTVANVILDAVGRHRIAYLPHDAY
                     YRDLSDLPPVQKAEINFDHPNSLETELMSDHVKELKAGNAVNIPVYDFSTHSRTSKTI
                     HVAPQRVIIVEGILIFAEKKLRELFDMKLFVDTDPDIRFIRRLQRDIAERGRTMDTVI
                     NQYLRTVRPMHLEFVDPSKRYADVIIPEGGMNQVAMDMVIARIESLLMKQG"
     gene            1697..2239
                     /locus_tag="PLL88_09080"
     CDS             1697..2239
                     /locus_tag="PLL88_09080"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOJ01759.1"
                     /translation="MEKRNLRQIITSALILFSLIVFSIYLFLHWREIQRFEHYGYFGV
                     FLISLLSNSTIIFPLPGVLLTSAMGAVFNPFWVAIAAGSGAALGEVSGYLAGVAGKFV
                     VEKEDWYERIMKAMQKYGVWMILLLAIIPNPFFDLAGIAAGVLRIPLWKFFLFCGIGK
                     IIKMLVFSYSGSSLSQIFLK"
     gene            2253..3617
                     /locus_tag="PLL88_09085"
     CDS             2253..3617
                     /locus_tag="PLL88_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ01760.1"
                     /translation="MSSSFEKMDRSLEKNLDSSIEELKRLVAQKSISAQNIGLSECAQ
                     LVAGMLEKRGFQVKVNPTAGAPIVTAEKKGKSPKTLLFYDHYDVQPPEPLELWLSEPF
                     KPEIRDGMLFGRGVSDNKGNIVNRLFAIDAILETAGELPCTVKFLIEGEEEVSSANLE
                     DFVFSHTQELAADACIWESGGVDQNDVPTQYLGLRGICYVELEVETASMDVHSGLGGT
                     IFPNAAWRLVWALNSLKNEEEEVLIKGFYDDVLPPPDEDLLFLKALPDTAEEYRTRYG
                     VKEFLLGMQGGMDLNFAEVYKSSCTICGLSSGYQGKGSKTVLPSKASAKIDFRLIPAQ
                     KPEKILELLRNHLDAHGFEDITITYLGGESPSKTSASDPFVQLVVNTARDVYGMPMQI
                     VPMSGGSGPNFIVQEALHLSIASLGAGYPGTGAHSPNENIRIADYLKSAKHIVRVLDA
                     FGRE"
     gene            complement(3705..5333)
                     /gene="treS"
                     /locus_tag="PLL88_09090"
     CDS             complement(3705..5333)
                     /gene="treS"
                     /locus_tag="PLL88_09090"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR02456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HOJ01761.1"
                     /translation="MHKDQTSWYKNAVFYEVSTRAYFDSNGDGIGDLKGLTQKLDHIK
                     HLGVDCIWLLPIFPSPLIDDGYDISDYYNVHPDLGSIEDFEELVTETHKRGMRIIADL
                     VVNHTSDQCKWFKQAEADPHSPYHDYYVWSDSNKRYADARIIFVDTESSNWAWSEKAQ
                     KYYWHRFYSSQPDLNYDNPAVREEMKNVMRFWLDMGIDGFRADAVPYLFEREGTNCEN
                     LPETHAYLKELRRLIDEEYPDRILLAEANQWPQELREYFADGDEFHMAFQFPLMPRIY
                     KALAKSDCSPIIDILKMTPQIPENCQWCTFLRNHDELTLEMVTEEDRQFLWDFYAPLP
                     RMRQNLGIRRRLAPLLDGNCQKIQLLNAVLFSLPGTPIIYYGDEIGMGDNIYLEDRDG
                     VRTPMQWSDEKNAGFSTAMEEKLVLPVVKSDQFSYTRINVAAQQADPNSLFHTMCKML
                     AIRKANPVMAVGEYEFVVQADFPALLFIKRNDGNNTILALHNLSAAAMPVDLEAHVPK
                     GMQMIDLMKKEAVRERTIQLAPYAFLWLKAEAKQ"
     gene            complement(5385..6575)
                     /locus_tag="PLL88_09095"
     CDS             complement(5385..6575)
                     /locus_tag="PLL88_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOJ01762.1"
                     /translation="MSVHRTARKHGLRNLPVHIILIGLCAIWLIPTIGLLITSFRPVQ
                     DINSSGWWTIFSKPKGYEEYQTYCSVCHGDDGSAIPEADLSDPDLVQNYRRSFSLLPS
                     LSREIDGQPHMADSSVPDETQTAAIISYMRRISGLDSSSRLTLDNYIDAFTGYRGNNT
                     YRADCASGKQSVDLYCDIRDLLNPRGMGRAFMNSLFVAVPSTVLPILIASFAAYAFSW
                     MDFKGRQWLFALLVGLQVVPLQMTLIPVSRLYAQLGMNGTFLGIWLFHTGFGLPYAIY
                     LIRNFMGGLPREIFESAYIDGASHWTAFWKLCMPLSIPALASLTIFQFLWIWNDLLVA
                     LVFLGGQHPVLTYQIGNMVTSLGAGWHLLTAAAFISMALPMLLFLLLQKYFVRGMLAG
                     SVKG"
     gene            complement(6572..7495)
                     /locus_tag="PLL88_09100"
     CDS             complement(6572..7495)
                     /locus_tag="PLL88_09100"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOJ01763.1"
                     /translation="MSKNSSGIMRQKPMTPWMYLLPALIVMITFIVYPGFNTFYLSLR
                     NNDNSGWASTRCLAGQPCWGVFENYRYALTSPIMQTAFLNNLKWIILMVSGTVVLGLL
                     IAVLADRVKYEALAKSLIFLPMAISFVGAGVIWKFMYNYGSSQVQIGVLNAIITALGG
                     QPVSWLTEPQINTVALIVVGVWMWTGFCMTVLAAALKGVSEEILEASRVDGANEWQVF
                     WKIMVPQILPTITVVITTMVINVLKIFDIVYVMTGGNYGTEVIANRMYIEMYKNYDPG
                     RASAIAVILIIVIIPVMIMNIRRFQRQEEMR"
     gene            complement(7595..>8917)
                     /locus_tag="PLL88_09105"
     CDS             complement(7595..>8917)
                     /locus_tag="PLL88_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ01764.1"
                     /translation="GEKMKKRFWLPIALLAIFAMVMAACQTAEEAAPVEEAAPAEETV
                     VEEGLCAGVQPGDEITLLYQWSGTEEEKLNAILAPFVEECGVVLKPESTRDQALLDTK
                     VQAGTPPDVAFWNVAQLEQYKDTLVPMDTLGANGDNYADFFKDPGTIDGVWLGLPVKA
                     DIKTIIWYSPAVFAAKGYSVPATWAELDALVEQMVANGDVPWSMGMESGDATGWTGSD
                     FIQDILLVQQGPDYVLSLISGEIPYNDAGVKQAYETYGKWAADPLYTVGGAQGTLSTG
                     FNDAILKVFSDPPEAMMVKQSGFAGGTILEQYPDMVYGEDYDFFPVPGAQGMQGGADW
                     LMVFNDTPAVRALIAYLSSLEGGANWAKASFDLSPNKGAEGNYVDPALIKKGAALANA
                     TGFTPDLGDTIPGGFGSAEWAAIVSYVNGGDLDAALASVALAQSEALQ"
BASE COUNT         2097 a         2214 c         2454 g         2152 t
ORIGIN      
        1 cgggatcatc ggacccaatg gggcaggaaa aagcatgctc ctgcgcatta tctgtgggct
       61 ggtgaaaccc tcgcgggggc aggtggtggt gttgggagag caggttgggg tcgattgtga
      121 tttcccgtcc agaaccggag cgttgatcga tattccgggt tttttgccgg cgtactctgc
      181 gcaaagaaac ttgcgattgc tggctgatgt cagtggaagc gttgaccaaa accgcattgc
      241 agaggtgctg tcaattgttg gattgaatca taaaaacacg cagccggttt ctacctattc
      301 agtcggtatg cgcaagagac tgggcattgc acaggcaatt ttagagcagc cggagttgtt
      361 gttgctggac gaaccaaccg attccattga ccaggctggc tggaagagtg tatatgaata
      421 tctgatccaa ctaaaagaag ccgggaccac cattcttctg accagcaaca atttggatga
      481 gatcacgatt ctttgcgatc agacatttct gttgaaagat cggcatctgg ctcctgttcc
      541 gtgatggctg gaaatagaaa gtgagtcacg aattagaaag tggtggcgct gtggctggct
      601 tgagtttcat gggcagcagt ttgtttggaa tgttgcggat ggtgaatttg tagagaaggg
      661 aaatgctatc cttgctgctg acacaatcag ggtagaaccc tgcactggtt gccttctctt
      721 tgcagccttc cagggcggcg ctctgaatta ccctccgccg atgggggtca tgaaagaaac
      781 cgtgcagccg ccggggatcg catcttcatc gatggcgatg cggttatcga tcagaataag
      841 gatattgcgc aggtgcgttt ctaaagcggg atactgcaag gtgacctgtt ccttcaggtc
      901 agcgatggtt gcgccgggcg cgatctccag cgctgcgctc gcgacacctg ctttttcttt
      961 caaatgagaa aagaatttga ccgtaatggt gttcatctgg aaccttgggt acccatgata
     1021 tactgcttca aacgggtgtg aagtataatt ttatcatgca aatcgactat gttcccattg
     1081 ttattggtat agccggcggc actggatctg ggaaaaccac cgtagcgaat gtgatattgg
     1141 atgcggttgg caggcatcgc atcgcttacc tgccgcacga cgcttattac cgcgacttga
     1201 gcgatctccc tccggtgcag aaggcagaaa tcaactttga ccatcccaac tcgctggaaa
     1261 ccgaattgat gagtgaccat gtcaaggagt tgaaagcggg aaatgccgtg aatattcctg
     1321 tctatgattt ttctacccac tcgcgcacca gcaaaacgat ccatgttgcg cctcagcgcg
     1381 ttatcatcgt ggaaggcatt ctgatcttcg ccgaaaagaa gttacgggaa ttgttcgata
     1441 tgaaattgtt cgtagatacc gatccagata tccgcttcat ccgccgactt cagcgcgata
     1501 tcgccgaacg cggcaggacc atggacactg tgatcaatca gtatctgcgc accgtgcgcc
     1561 ccatgcacct ggagttcgtc gatccatcca agcgctacgc cgatgtgatc atccccgaag
     1621 ggggaatgaa tcaggtggct atggacatgg tgattgcgcg catcgaatca ctcttgatga
     1681 agcagggatg acccccatgg aaaagaggaa tctacgccag attatcacca gcgcgttgat
     1741 ccttttctct ctgatcgtct tctcaatcta tctttttctt cactggcgcg aaattcagcg
     1801 ttttgaacat tacgggtatt tcggcgtctt cctgatttca ctcctttcca attccaccat
     1861 cattttcccg ttgcctggtg tgctgctcac ctctgccatg ggagcagtct tcaatccatt
     1921 ctgggtggcg atcgctgccg gctcaggtgc ggcgctgggg gaggtctcgg gctatctggc
     1981 gggtgtggcg ggaaagttcg tagtggagaa ggaagactgg tacgaacgga tcatgaaagc
     2041 tatgcaaaaa tatggagttt ggatgatcct gctgctggcg atcattccca acccattttt
     2101 tgacctggcg ggaatcgcag caggagtgct gcgcattccg ttatggaagt tctttttgtt
     2161 ctgtggaata ggcaagatca tcaaaatgct ggtcttctcc tattccggct catcgctctc
     2221 gcaaattttc ttgaagtaga aaggaaagca gaatgtcatc atcgtttgaa aaaatggacc
     2281 gatctctgga aaagaatctg gattccagca ttgaagagtt gaagaggctg gtagcgcaga
     2341 aaagcatttc tgcgcaaaac atagggttga gcgaatgcgc gcaactggtt gccggcatgc
     2401 ttgagaaacg gggatttcaa gtgaaggtca atccaaccgc cggcgcaccg attgtcacag
     2461 ccgaaaagaa aggaaaaagc cctaaaacgc tgctgttcta tgaccactac gacgtacaac
     2521 ctccggaacc attggaacta tggctgagcg aacccttcaa acccgaaata cgggatggta
     2581 tgctctttgg aaggggtgta agcgataaca agggcaatat cgtcaaccgg ctcttcgcca
     2641 tcgatgccat tctggagact gccggcgaat tgccctgcac cgtcaaattc ctgatcgagg
     2701 gagaagaaga agtgagcagc gcgaatcttg aagatttcgt gttctcgcac acgcaggagc
     2761 tggcagccga tgcctgcatt tgggaatcgg gcggggtgga ccagaatgat gtgcccaccc
     2821 aatatctggg tttgcgcggc atttgttatg tggagctgga ggtggaaacc gccagcatgg
     2881 atgtgcattc agggttaggt ggaaccatct ttcccaatgc tgcctggcgg ctggtttggg
     2941 cgttgaactc tctcaagaac gaggaagaag aagtcctgat taagggtttc tacgatgatg
     3001 ttctcccgcc tcctgatgaa gacctgctat ttctcaaagc attgcctgac accgctgagg
     3061 agtatcgcac ccgttacggg gtcaaagaat tccttttggg aatgcaggga gggatggatt
     3121 tgaactttgc agaagtgtac aaatcttctt gcaccatctg tggattgagc tctggttatc
     3181 agggtaaggg ttcaaagacc gtgctgccgt ctaaagcgtc cgccaagatc gatttccgtt
     3241 tgatccccgc tcagaaaccg gagaagatcc tggagctgtt gcggaaccac cttgatgcgc
     3301 atgggttcga ggatatcacg atcacctatt tgggcggaga atcaccttcc aagaccagcg
     3361 ccagcgatcc ctttgtgcaa ctggttgtga acacagcccg cgatgtatac ggaatgccca
     3421 tgcagattgt gcccatgtcg ggcggttcgg gtccgaactt catcgtgcaa gaagccctgc
     3481 acctctccat cgcctctttg ggggcgggtt atccagggac gggcgcccat tcgcctaatg
     3541 aaaacatccg cattgccgat tacctgaaaa gcgccaaaca catcgtgcgc gttttggacg
     3601 ctttcggaag ggaataggat cgggcgaagc ccttcttgga aaatgacagg cagggaatgc
     3661 ggttatagca ttccctgcct gttttttggt agaacttggc aaaactattg ttttgcctca
     3721 gctttcaacc agaggaacgc gtagggcgca agctggatgg tgcgctcacg cacagcttct
     3781 tttttcatca ggtcgatcat ttgcattccc ttgggcacat gcgcttccag gtcgactggc
     3841 atggcggcag cagaaaggtt gtgcagtgcc aggatggtgt tgtttccatc gttgcgtttg
     3901 ataaaaagaa gagccggaaa gtctgcctgt accacaaatt catactctcc caccgccatg
     3961 accgggttgg ctttgcggat agccagcatt ttgcacatgg tgtggaacag ggagttgggg
     4021 tcggcttgct gcgctgccac gttgatgcgc gtgtatgaga attggtcgct cttgaccacc
     4081 ggcagaacca gcttttcttc catggcggtg gaaaaaccgg cgttcttctc gtcgctccac
     4141 tgcatgggtg tgcgcacccc gtcacggtct tcgaggtaga tgttatcccc catcccaatt
     4201 tcatccccat aataaataat gggggtgccg ggaagcgaga aaagtactgc gttcagcaac
     4261 tggatcttct ggcagttccc atccagcagg ggggcgagcc ggcggcgaat gcccaggttc
     4321 tggcgcatcc tgggcagtgg ggcgtagaaa tcccataaga actggcgatc ttcttctgtg
     4381 accatttcaa gggtcagctc atcatgattg cgaaggaagg tgcaccactg gcagttctcc
     4441 gggatctgag gggtcatttt caagatgtcg ataatgggag agcaatcgct ctttgccagg
     4501 gctttgtaaa tacgcggcat caggggaaat tgaaatgcca tgtggaattc gtcgccatcg
     4561 gcgaaatatt cccttaattc ttgtggccat tgattggctt cagccagcaa gatgcgatca
     4621 ggatattctt catcgattaa tctgcgcagc tctttgagat aggcgtgcgt ttctggcagg
     4681 ttttcgcaat tggttccctc ccgttcgaag agatatggga cagcgtcggc acggaagcca
     4741 tcgatgccca tatcgagcca aaaacgcatc acgtttttca tctcttcgcg aactgccgga
     4801 ttatcgtagt tcaagtcggg ctggctggaa tagaagcgat gccagtaata cttctgggct
     4861 ttttcgctcc acgcccaatt ggagctttcg gtgtccacaa agatgatgcg tgcatctgca
     4921 tagcgcttgt tggaatcgct ccagacgtaa taatcatggt aaggcgagtg agggtcggct
     4981 tctgcctgct tgaaccactt gcattggtct gaggtgtggt tcaccaccag gtcggcgatg
     5041 atgcgcatgc cgcgtttgtg tgtttctgtg accagttctt cgaaatcttc aatgcttcca
     5101 agatcgggat ggacattgta gtagtcagaa atgtcgtatc cgtcatcgat cagcggggag
     5161 gggaagatgg gcagcagcca gatgcaatct accccgaggt gtttgatgtg atccaatttt
     5221 tgggtgagcc ctttgagatc accaattccg tctccgttgg aatcaaaata agcgcgggtg
     5281 gagacttcgt aaaagacggc gtttttatac cagctagttt gatctttgtg cattcgttat
     5341 cctcaacaat tcactgattt cggagcaaca tctctttggc tgatctatcc tttgaccgaa
     5401 ccagccagca taccgcgcac gaagtatttc tgcaacagca agaagaggag catggggagc
     5461 gccatggaaa tgaaggcggc tgcggtgagc aggtgccaac ctgcgcccag tgaagtgacc
     5521 atgttgccga tctgataggt caacacgggg tgctgaccgc ccaggaacac caatgccacc
     5581 agcaggtcgt tccagatcca taagaactgg aagatagtga gcgaagccag agccggaatg
     5641 gaaagcggca tgcatagctt ccaaaaagcc gtccaatgcg aagcgccgtc aatgtaggca
     5701 gattcaaaga tctcgcgggg taagccgccc atgaagttcc tgatcaggta gatggcgtag
     5761 ggtaagccaa agccggtatg gaaaagccag attcccagaa aagtgccgtt catgccgagc
     5821 tgtgcgtata gccgtgatac gggaatgagc gtcatctgca gcggtaccac ctgcaacccc
     5881 accagcaacg cgaatagcca ctggcgcccc ttgaaatcca tccacgaaaa ggcgtaagca
     5941 gcaaaagagg cgatcagaat ggggagaact gtagaaggga cggcaacgaa caagctgttc
     6001 ataaaagctc tgcccatgcc gcgcgggttc aggagatcgc ggatatcgca gtacaggtct
     6061 accgattgct tcccgctggc gcaatctgcg cgatacgtgt tgtttccgcg ataaccggtg
     6121 aaggcgtcga tatagttatc gagggtgagc ctgctactgg aatccagccc cgatatacgc
     6181 ctcatgtaag aaatgatggc ggcggtctgc gtttcatccg gcacggagct gtctgccatg
     6241 tgaggttgcc catcgatctc tctgctcaaa gatggcagca gggaaaacga gcggcgataa
     6301 ttttgaacga gatccgggtc tgataggtcc gcttcgggaa tggcggaacc gtcatcccca
     6361 tggcagacgg agcagtaggt ttggtactct tcatatcctt tgggtttgga gaatatggtc
     6421 caccaaccgc tgctgttaat atcctgcacc ggtctgaagg aggtgatcag caaaccaatg
     6481 gttgggatca accagatggc gcacaagccg atcaagatga tgtggacggg tagattcctc
     6541 aagccatgtt ttcgagcggt tctatggacg ctcatcgcat ctcctcctgt cgctggaagc
     6601 ggcggatatt catgatcatg acgggaatga tgacgatgat cagaatcacc gcaatggcag
     6661 aagcgcgccc gggatcatag ttcttgtaca tttcgatgta catgcggttg gcaatcacct
     6721 ctgtgccgta gttcccgccg gtcatgacgt agacgatatc aaagatcttc aaaacgttga
     6781 tcaccatggt cgtgatcacc acggtaatgg tcggcaagat ctgtggaacc atgattttcc
     6841 agaacacctg ccattcgttg gcaccatcta cgcgagaggc ttccagtatt tcttcagaga
     6901 cccctttgag ggctgccgcc agcacggtca tacagaatcc tgtccacatc cagacgccga
     6961 ctacgatcaa agcgacggtg ttgatctgcg gttccgtcag ccatgaaacc ggttgtccgc
     7021 ctagtgcggt tatgatggcg ttcagcaccc cgatctgcac ctggctggaa ccgtagttgt
     7081 acatgaattt ccagatcacg ccggcgccaa cgaaagaaat agccatgggt aaaaagatga
     7141 gggatttggc gagagcttca tatttcaccc ggtcagccag cactgcgatc agtagtccaa
     7201 ggacaaccgt gccgctgacc atgaggatga tccatttcaa attgttcagg aacgcagtct
     7261 gcatgatagg ggaggttagt gcgtaacggt agttttcaaa aactccccag caaggctgcc
     7321 ccgccagaca gcgcgtgctc gcccaaccgg aattatcgtt gttgcgcaaa ctgaggtaga
     7381 aggtattaaa accaggataa acaatgaagg tgatcattac gattagggca ggcaacaggt
     7441 acatccaggg tgtcatgggc ttctgacgca tgatgccaga agaattttta ctcatttttc
     7501 acctccatgt tccttttata gcatgattaa acatgaaatg attgcttaaa aagatcgggc
     7561 gtctggttca cagacacccg atcacaggtt gtacttattg tagtgcttcg ctttgggcta
     7621 gggcgacaga agcgagcgcc gcatccaggt caccaccgtt cacataactg acaatggctg
     7681 cccattcggc gcttccgaac ccgccgggga tggtatcgcc aagatcaggg gtgaagccgg
     7741 tggcgtttgc cagggctgcg cctttcttga tcaaagctgg atcaacgtag ttgccttcgg
     7801 cacccttgtt gggtgaaaga tcgaagctgg ctttcgccca gttggcaccg ccttccagcg
     7861 atgagaggta ggcgatgaga gcgcgaacag ccggagtgtc gttgaagacc atcagccagt
     7921 cggcaccacc ttgcataccc tgagcgccgg gaaccgggaa gaaatcgtaa tcttcgccgt
     7981 acaccatgtc aggatactgc tccaggatgg tcccaccagc gaagccggat tgctttacca
     8041 tcatcgcctc tggcggatca gagaagacct tcaggatagc atcgttgaaa ccggttgaga
     8101 gagttccctg cgcaccaccc acggtgtaca gcggatcggc tgcccatttg ccgtaggtct
     8161 catacgcctg cttcacacct gcgtcattgt aggggatctc accagagatg agactcagca
     8221 catagtctgg tccttgctgt accagcagga tatcctggat gaagtcggaa ccagtccaac
     8281 cggttgcatc gccagattcc atgcccatgc tccagggcac gtcaccattg gcaaccatct
     8341 gctccactaa ggcatctaat tctgcccagg tggcgggtac gctgtatccc ttggctgcaa
     8401 acacagccgg gctgtaccag atgatggtct tgatatcagc tttcactggc aaaccaagcc
     8461 atacaccgtc gatggtcccg ggatccttga agaaatcggc atagttatcg ccatttgctc
     8521 ccagggtatc catggggacg agggtgtctt tgtattgctc aagctgggcg acgttccaga
     8581 aagccacgtc tggtggtgtg ccagcctgta ccttggtgtc gagcagagcc tgatcgcggg
     8641 tggattcggg tttcagcacg accccgcatt cttccacaaa gggagccagg atggcattga
     8701 gtttttcctc ttctgtgcct gaccactggt acagaagtgt gatctcatcg ccaggttgaa
     8761 cgcccgcgca gagaccttct tcgaccactg tttcttctgc cggagccgct tcttctacgg
     8821 gtgcagcttc ttcagcagtc tggcaggctg ccatgaccat tgcgaaaatg gctagcagtg
     8881 cgatcggtaa ccaaaatctt tttttcattt tttctcc
//
LOCUS       DAOSDS010000086         8752 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_2987_length_8752_cov_3.592733, whole genome shotgun sequence.
ACCESSION   DAOSDS010000086 DAOSDS010000000
VERSION     DAOSDS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8752
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2987_length_8752_cov_3.592733"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2355)
                     /locus_tag="PLL88_09110"
     CDS             complement(<1..2355)
                     /locus_tag="PLL88_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ01765.1"
                     /translation="MGNFRDHEYSDRDFQEHVAWVYPMPAKSADFVGFPDSISPPIID
                     FFHTQGISSLYSHQARALDELANHHHVVITTGTSSGKSLIFQVSVLQQWLREPNSTAL
                     LLYPTKALANDQLVPFKEINNHLRRMGTEYLPGVAIYDGDTSQSNRITIRNNASILLS
                     NPDMVHLSMLPHHTMWKRFFSNLRFIVIDEVHIYKGVFGSHFANVMRRLKRVVQFYGG
                     NPLFICTSATIHDPKAFVEKLLECPFSVIAEDGSAHGEKNVYFYNPPIVDEQLNIRRG
                     ILEEGLKIAKLSIQNDLQTLVFVRARRSVEILLRRAKEKLPAGSMVGYRSGYLADERR
                     KIEGDLKNGTIACVVATNALELGIDIGGMDRVVLLGYPGSIASFIQQIGRAGRKTNSS
                     SAVFVASMDPLDQYLLQDPAYILTNTPEQPLIDPQNMLILFNQLRCAAYELPFQKSDH
                     FGGISAEVLQGYLDVLCLRGDLVERDGKYYWVGEAYPAADISIRSISTVPFDLLLREG
                     NSSRKIGEVDGESVLWMAHPGAIYLQAGETYLVQDLALDEHKVFLEEGDFPYYTNPKE
                     QQDIQIQDVLACDTRESASFSFGNVTVTSQVVGYDRLDWVSNEIIESVGLDLPPSDLS
                     TQAFWMVLSEKTVESLRKNALWYSDPNDYGKNWAAIRQQVLARDARRCSLCGKQGSSI
                     LKLHVHHKRPFRTFADPTIANDLSNLITLCEVCHQRVEQTVRIHSGLGGLGYIFSHLA
                     PLYLMCDLRDLGLFIEPRWKPAGYAPVIMLYDAFPGGIGLSDALH"
     gene            2484..3815
                     /locus_tag="PLL88_09115"
     CDS             2484..3815
                     /locus_tag="PLL88_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ01766.1"
                     /translation="MKEKRNPKYSNKTLLIVIFFAFMLLHQTDKLLINPMGDLIINEF
                     GLTDTQWGSVATAALIVGSICYPVWGYLYDRYSRGKLLALAALIWGSTTWLSAIAPTF
                     PLFVASRATTGIDDSSYPGLYSLIADYFVPKVRGKIYGLLQLTMPLGYMIGLVISMTV
                     AGGIGWRKVFYVTGGLGVLLSLIIFLFVKDIPRGRSEPELADVKDVPELQKFHFEWSK
                     VKDVVRKPSLIFMYLQGFFGVFPWNTIVAFIFLYLGQERGYSDTQVLLTMVPAILILA
                     LGYPVGGMVGDYFFSKSKKGRVYVAMVGVILGAIFLYFTLNVPLENTTLFAILLGVTA
                     LFIPFASPNVTSIVNDVTLPEMRSTATAIQYFIESSGAALAPLLTGIISDSLRAAGNP
                     YPRGTAILIICTTTWALCGIFFFITSKFIEKDVNSLHSSLQQRAQAITGKK"
     gene            complement(3881..5242)
                     /locus_tag="PLL88_09120"
     CDS             complement(3881..5242)
                     /locus_tag="PLL88_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01767.1"
                     /translation="MRQKSRFSIFFTLFLATATLIASSGCAAPSQGYTADPIYAEVVF
                     EATLPQALAANTTLQLEILDEISCLNFNPTRYDMSLKGESTYYVRLPLPIDSIVKYRY
                     ILNTSPATSEKDMEGNDILYRMVKISDPVVVQDTVANWENAASTSAKGVLSGYIVDAT
                     NNIPLPSVLVTIAGKQTYTATDGSFTIENVPVGEHAMLAYHPSGAYQVFQQKAVIADG
                     AQTPAVFGMQPAQYVNVSFHVDTPDNTVKGAPLRLLGNLLQLGNTFSELNASCSVLAS
                     QAPIMSYQEDGSYLLTLQLPVGMDIRYKYSLGDGFWNAERKKDSSLRVRQLVVPDHDI
                     IVYDTISTWLVSDEGPLTLRVKVPATTPATDSISLQLNPYVWMNPLPMWALGNHEWLL
                     TIYSPLDVLANATYRYCRNEQCDISEGIIASSGTAISVITTISGTINDEVLQWNLALP
                     SPN"
     gene            complement(5267..5704)
                     /gene="nusB"
                     /locus_tag="PLL88_09125"
     CDS             complement(5267..5704)
                     /gene="nusB"
                     /locus_tag="PLL88_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOJ01768.1"
                     /translation="MKPRTEARGLALQILYEYDLSRHPAGEILMNRLQEDPKDEGLID
                     FARQITNGVIPISQKLDIIIAEHAPEWPLEQISSIDKNIIRIALWEFAVSKITPVKVA
                     INEAVELAKVYGSDSSPRFVNGVLGSLSTQVAQITALFSDLSE"
     gene            complement(5711..6073)
                     /locus_tag="PLL88_09130"
     CDS             complement(5711..6073)
                     /locus_tag="PLL88_09130"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOJ01769.1"
                     /translation="MNTTEELLGKTTIDPNVILTIIRMTALRVSGVCRMASGPAGLDD
                     LVKKYYSDGVKIDVEDHTVYIDLYLVLYRDVDLRATARKIQKDVQRAVEDMVGMSVAS
                     VDIHIDDIVIRNNTKETA"
     gene            complement(6141..7379)
                     /gene="larA"
                     /locus_tag="PLL88_09135"
     CDS             complement(6141..7379)
                     /gene="larA"
                     /locus_tag="PLL88_09135"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HOJ01770.1"
                     /translation="MMKKELSLPYGSGKITLQLPEKVAQCDVFHPPVTQKAPYTAEKV
                     YDLLQKSPLTLTSKKRVAIAINDKTRPVRYDVLLTPLLHYLQEAGVPHEAITLFISSG
                     THQTIPTSEYPEFIPPEVVARYAIVCHDCDDASQLLDVGTTMAGTPVLINRRFYEADI
                     KITLGTIEPHHFMGFSGGAKTAAIGLTGRSTIEANHRLLLQPYTVTGEYERNPMRQDV
                     EEIGERIKITACLNAVMNYKKDVLEVLWDTPREVMKKGIPESRRLTQVVIPPMYDLVI
                     ASAGGFPKDINLYQAQKALTNACMVCQPGGSILLAAECREGHGNQKYADFMQGKTSFQ
                     QVLDEFGKVPFQIGPHKAYLIARQGLKYRMYLQSGMADADVRDLLLTPIHSIDEYISH
                     TDLSGMRIAILPYATTTVPC"
     gene            complement(7376..7627)
                     /locus_tag="PLL88_09140"
     CDS             complement(7376..7627)
                     /locus_tag="PLL88_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01771.1"
                     /translation="MWGNCGNAGKVSPNRQPGLFKINTLPSPIIENYGKDLFPSGQTG
                     TGSTEKAIHSKIGTINPWSHKIVVNSINAIRNTEYFKPT"
     gene            7773..>8752
                     /locus_tag="PLL88_09145"
     CDS             7773..>8752
                     /locus_tag="PLL88_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ01772.1"
                     /translation="MSKFSTIESAINAYLDTVRLARSQHTYTTYRTALHNFTACLAKN
                     AVDASKYPVEQLNTDAIAWYANYLKQYAATTEQLYLTAVKGFYDFLAAEEAVELNLPK
                     VKLLIKQRARRPGRRLPQFPADSISRLLDYYEQKALPENQEQEIILREMRDKAFLVTL
                     ADSGLRVHEACGLRRGDIDWNEGKALIIGKGDKQAVVRFSKRSLRALKAYLQARSDLD
                     GSSGRPLTSLPLFARHDKGAGNKVKPITPTTGRNIVQERVAEALGADAVGTITPHSFR
                     HYFVTRVLLSTGNLKLAQNLARHQNIAVTQRYAHLADDELDSGYWQVFEE"
BASE COUNT         2173 a         2168 c         2179 g         2232 t
ORIGIN      
        1 gtgtaacgcg tcgctcaaac caattccgcc cggaaaagca tcatacagca tgatcaccgg
       61 ggcatatcca gccggcttcc agcgcggttc aatgaacaat cccagatccc gcaggtcgca
      121 catcagataa aggggtgcca aatgtgaaaa aatatacccc aaaccgccca acccggagtg
      181 gatgcgcacg gtttgttcta cgcgctggtg acagacctca cacaacgtga tcaaattgga
      241 cagatcgttg gcaatggtgg gatcagcgaa ggtgcggaag ggtcttttgt gatgcacatg
      301 cagtttaaga atagaactgc cctgcttgcc acacaaagaa caacgccggg catcgcgtgc
      361 cagaacctgc tgccgaattg ccgcccagtt cttgccataa tcgttgggat cgctgtacca
      421 cagggcattt ttgcgcaggc tttccacggt tttttccgaa agaaccatcc aaaaagcctg
      481 cgtgctcagg tcggaaggtg gtaagtcgag ccccaccgat tctatgatct cattacttac
      541 ccaatccaac ctgtcgtaac cgaccacctg actggtcaca gtaacgtttc cgaaggagaa
      601 actggcgctt tctctcgtat cacacgccaa aacatcctga atttgaatat cctgttgttc
      661 tttggggttg gtataatacg gaaaatctcc ctcttccaga aacaccttat gttcatccaa
      721 tgcaaggtcc tgaacaagat aagtttctcc cgcctgcagg tagatagcac ccggatgcgc
      781 catccacaat acgctttcgc catccacttc cccgatcttg cgacttgaat ttccttctct
      841 caacaaaaga tcaaagggga cagtggagat gctgcggatg gaaatatccg cagccggata
      901 ggcttctccg acccagtaat atttcccatc ccgttccacc agatcacccc tcaggcataa
      961 cacatccaga tatccttgca acacctcagc ggaaatgcct ccaaaatggt cgcttttttg
     1021 aaaggggagt tcgtatgccg cgcagcgcaa ttggttgaac aaaatcagca tgttctgcgg
     1081 atcgatcaag ggctgttccg gcgtattggt taaaatatat gccgggtctt gcagcaaata
     1141 ctggtccaag ggatccatgg aagccacaaa taccgctgag gaagagttcg tctttcgccc
     1201 ggcacgcccg atctgttgga taaaggaagc aatactgccc ggatacccca ataaaaccac
     1261 ccggtccata ccgccaatat caattcccaa ctccaaagcg ttggtagcca ccacacaggc
     1321 gatcgttcca ttcttcaaat ccccttcaat cttcctgcgt tcgtcagcca ggtacccgct
     1381 gcggtagccc accatgctcc ctgcaggcag tttttccttc gccctgcgca gcagaatctc
     1441 caccgaacgg cgtgcacgaa cgaagaccaa cgtttgcaag tcattttgaa tggataattt
     1501 tgcaatcttc agaccttctt ccagaatgcc gcggcgaata ttcaactgtt catcgacaat
     1561 gggcggatta tagaaataga cattcttttc cccatgggct gatccatcct cggctatcac
     1621 tgaaaaaggg cattccaaca atttttcgac gaacgctttt ggatcatgga tggtagcgct
     1681 ggtacagata aaaagcggat ttccaccgta aaattggacg acacgtttca ggcggcgcat
     1741 gacattggca aaatgcgacc cgaaaacgcc cttgtaaata tgcacctcat cgatcacgat
     1801 gaaacgcagg tttgagaaaa aacgtttcca catggtatgg tggggcaaca tgctcaaatg
     1861 caccatatcg gggttggaca gaaggattga cgcgttattg cgtatcgtta ttctattcga
     1921 ctgcgacgta tccccatcat aaatggcgac acccggcagg tattctgtcc ccatccttcg
     1981 gagatggtta ttgatctctt tgaaaggaac caactggtcg ttggcaagcg ctttggtggg
     2041 gtagagcaag agagccgtgg aattcggctc ccgtaaccat tgttggagga cactgacctg
     2101 gaagatcaag gatttgccgc tggaagtgcc agtggtgatg accacatgat ggtggttggc
     2161 aagttcatcc agtgccctgg cttgatggga ataaagagat gaaatgcctt gcgtatggaa
     2221 aaagtcgatg atgggtgggg aaatcgaatc aggaaaaccc acaaaatccg cgcttttcgc
     2281 aggcataggg tatacccatg caacatgctc ctggaaatcg cggtcgctgt actcgtgatc
     2341 acgaaaattg cccattttta agaggaaaaa tcctttattt ccatgtgaaa ggtatttcgc
     2401 attcctttag tttattataa aatacactga cgggggaacg aaccagaatc gttaatctga
     2461 ccgttggagg taaaagagaa accatgaaag aaaaaagaaa tccaaaatac tcaaataaaa
     2521 ctttattgat cgtcatcttt tttgcgttta tgctactgca ccaaacagac aaactgttga
     2581 tcaatcccat gggagacctg atcatcaatg agttcggtct gaccgatacc cagtgggggt
     2641 cggtggcaac tgctgcgctg attgtgggat cgatctgcta tccggtatgg ggttatctct
     2701 atgaccgcta ttcacgcggg aaactgctgg cactggctgc cttgatctgg ggttccacca
     2761 cctggttgag cgcgattgca ccgacctttc cactttttgt cgcgtcacgc gccaccaccg
     2821 gcattgatga ttccagctac ccgggtttat acagtttgat cgcggattat ttcgtgccga
     2881 aggtgcgcgg caagatctac ggattgcttc agttgaccat gcccctggga tatatgatcg
     2941 gtctggtgat ctctatgacc gttgccggag ggataggctg gcgtaaggtc ttctatgtca
     3001 ccggtgggtt gggggttctg ctttccctca tcatattcct gtttgtcaag gatatcccgc
     3061 gcggcaggag cgaacccgaa ctggcagatg taaaggacgt gccggaactg caaaaatttc
     3121 atttcgaatg gagcaaggtg aaggatgtcg tcagaaaacc cagtttgatc ttcatgtact
     3181 tgcagggttt cttcggggtt tttccatgga ataccattgt agcttttatt tttctctacc
     3241 tgggacagga gcgaggatac agcgatacgc aggttttgct gaccatggtg ccggctatcc
     3301 ttattttggc attgggctac ccggtgggtg gcatggttgg agactatttt ttcagcaaaa
     3361 gtaaaaaagg gcgcgtgtat gttgccatgg tgggcgtcat tttaggtgct attttcctat
     3421 acttcacgct caatgtgccg ctggaaaata cgaccctgtt tgcgatcctg ctgggggtca
     3481 cagctctgtt catccccttt gcctctccca atgtaacgtc tatcgtgaat gatgtcacgc
     3541 tacccgaaat gcgcagtacc gccactgcca ttcaatactt tattgaatca agcggcgcag
     3601 cactggcacc tctgcttacc ggtatcatct ccgactcgtt gcgcgcggcg gggaatccat
     3661 atcctcgcgg aacagccatc ttgatcatct gcacaacaac ctgggcgtta tgcggtatat
     3721 tcttctttat cacgtccaag ttcatcgaaa aggatgtgaa cagcctgcat tcatccctgc
     3781 agcaaagagc gcaagcgatc accgggaaaa aataacgaaa aaacggtacg gaaaaatcga
     3841 tctccgtacc gtttttcatt tcttattctg tttcattctg ctaattcgga gatggcaaag
     3901 ccaggttcca ttgcagcact tcgtcattga tcgttccgct gatggttgta atcacagaaa
     3961 tagcagttcc actgctggca atgatgcctt ctgagatgtc gcattgttca ttgcggcagt
     4021 agcggtaggt ggcattagcc agcacgtcca gcgggctgta gatggtcagc agccattcat
     4081 gattacccaa tgcccacatg ggcagtggat tcatccagac ataggggttc aattgtaggg
     4141 aaatgctgtc ggtggctggc gtggtggctg gcacttttac gcgaagggtg agcggtcctt
     4201 catcgctgac cagccaggtg gaaatggtgt cgtaaacgat gatatcatga tcgggcacca
     4261 ccaattgacg cacccgcaaa ctgctgtcct ttttcctttc agcgttccaa aaaccgtcac
     4321 ccagagaata tttatagcga atatccatgc caactggcaa ctgcaaggtc aggaggtagg
     4381 aaccatcttc ctgatagctc atgatgggcg cctgagaagc cagcacggaa caactggcgt
     4441 tcaattcgga gaaggtattg ccgagctgta gaagattgcc aagcaggcgc agcggagccc
     4501 ccttgaccgt gttatcggga gtgtcaacat ggaagctgac attcacgtat tgagctggtt
     4561 gcataccaaa aacagccggg gtttgtgcac catcagcgat caccgccttt tgctggaaca
     4621 cctggtaggc gccggagggg tggtatgcca gcatagcatg ttccccaacc ggcacatttt
     4681 cgatggtgaa agaaccatcc gtggcagtat aggtttgttt tccggcaatg gtgaccaata
     4741 ctgagggcag gggaatatta ttggtggcgt caacgatata cccagagaga acgccttttg
     4801 cggaagtgct tgccgcattt tcccagttcg cgaccgtatc ctggactacc accggatcac
     4861 tgattttcac cattcggtag agaatatcat tcccctccat gtccttttca gaagtggcgg
     4921 gtgaggtgtt caaaatataa cgatatttta cgattgaatc aatgggcaag gggaggcgca
     4981 cgtaataggt gctttcccct ttcagactca tgtcgtagcg cgttggattg aagttcaggc
     5041 agctaatctc atccagaatc tccaattgaa gtgtcgtgtt agctgccaac gcctgcggca
     5101 aagtcgcttc aaagaccact tctgcgtaga taggatcagc ggtatagccc tgactgggag
     5161 cagcacaacc gctcgaagcg ataagagttg ctgtagcaag gaaaagtgta aaaaaaattg
     5221 aaaatcggga tttctgtctc ataatcaatt cctgaaatta ttccttttac tctgaaaggt
     5281 cgctgaacag ggctgtaatt tgcgccacct gggtagaaag actgcccagc acgccattga
     5341 caaagcgcgg cgaagaatca gagccgtaca ctttggcgag ttccacggct tcattgatcg
     5401 ccacttttac cggtgtgatc ttggaaactg caaattccca caatgcaatg cgaatgatgt
     5461 tcttatcgat agaagaaatc tgctccagcg gccattcggg ggcatgttcg gcgataatga
     5521 tatccaattt ttggctgata gggatgacac cgttcgtgat ctggcgggcg aaatcgatca
     5581 agccctcatc tttgggatct tcctgtagcc gattcatcaa aatctcgccg gctgggtgcc
     5641 tggagaggtc atattcatag agaatttgca atgccaaccc tctcgcttct gtcctcggtt
     5701 tcatggttga ttacgctgtt tctttggtgt tgtttcgaat cacgatgtca tcgatatgga
     5761 tatccacgct cgccacgctc attccaacca tatcttccac ggcgcgctgc acatcttttt
     5821 ggatcttgcg cgcagtcgcc cttaaatcta catcgcgata aaggacaaga taaaggtcaa
     5881 tatagaccgt gtgatcttcc acatcgatct tgacgccgtc ggagtaatat tttttcacca
     5941 gatcgtccaa tccagccggt cctgatgcca ttctgcacac gccagagaca cgcagcgcag
     6001 tcatgcgaat gatggtcagg atcacattgg ggtcaatggt tgtttttcca agcaattctt
     6061 cagttgtatt catcgaatct ccaatttccg gattcagtcc tttcaattat aacccggttc
     6121 gttccgaaag gttttcctgc ctagcagggt acagttgtcg ttgcataggg taagattgca
     6181 attctcatgc cagaaagatc ggtgtgggaa atatattcgt caatggagtg gatgggggtt
     6241 aacagaaggt cgcgcacatc ggcatctgcc atgccggatt gcaaatacat ccggtatttc
     6301 aagccctggc gggcaataag gtaagcttta tggggaccga tctggaaggg cactttgccg
     6361 aattcatcga gaacctgttg aaaactggtt ttgccttgca taaaatcagc gtatttttga
     6421 ttgccatgtc cttcgcggca ttcagcggct aggagaatgc tgccgcctgg ttggcagacc
     6481 atacaggcat tggtcagcgc tttctgcgcc tgatacagat tgatatcttt cggaaaaccg
     6541 ccggcagatg cgatcacaag gtcatacatg ggaggaatga caacctgggt caaccgtctg
     6601 ctttccggga ttcctttttt catcacttcc cgcggggtat cccacaggac ttccagaaca
     6661 tccttcttgt agttcatgac ggcattcaag cacgccgtga ttttgatgcg ctctccgatc
     6721 tcttccacat cctggcgcat aggattgcgt tcatattcgc ccgttacagt gtagggctgc
     6781 agcagaagac ggtgattggc ttcgattgtg ctgcgcccgg tcaatccgat ggcggcagtt
     6841 ttcgcacccc ccgaaaatcc catgaaatgg tgcggttcga tggttcccag cgtgatcttg
     6901 atatccgctt catagaagcg tctgttgatc agcacagggg tgcctgccat cgtcgtcccc
     6961 acatcaagaa gctggctggc atcatcgcag tcatggcaga cgatggcata gcgcgccacc
     7021 acttcagggg ggatgaattc cggatattcg ctggtcggga ttgtttgatg ggtgccagaa
     7081 gaaataaaaa gcgtgatggc ttcatgcgga acacccgctt cttgcagata gtggagcagg
     7141 ggagtaagca gaacatcgta gcgcaccgga cgggttttat cgttgatggc gatggcaact
     7201 cgctttttgg aagtgagcgt gagcgggctt ttctgcaaca ggtcatacac tttttcggca
     7261 gtatagggcg ctttttgggt cacaggtggg tggaaaacat cacactgtgc taccttttca
     7321 ggcaattgca aggtgatttt tcctgatccg taaggcaaag agagctcttt tttcatcatg
     7381 taggtttaaa atattctgta tttcgtatag cattaatgct atttaccaca atcttatgcg
     7441 accatgggtt gatagttcct atcttactat ggattgcttt ttctgtgctg ccggttcctg
     7501 tttgcccgga aggaaaaaga tctttaccgt agttttctat aataggagag gggagagtat
     7561 ttattttaaa aagacctggc tgtctgtttg gcgatacttt ccctgcattt ccacaatttc
     7621 cccacatgcc cgctcgtttt ccctgtaagc tttcggtttt tccctattgc agggtggtgg
     7681 tatttatgct aaaatagcat tatggtttcg ataagtacta ttatagaaag catatagaaa
     7741 agcaagggac accccagaaa gggttaaaaa caatgagcaa gtttagtact attgagagtg
     7801 cgatcaatgc ttatctggac accgtgcggc tggcacgatc gcaacatacc tacacaacct
     7861 atcgcactgc acttcataat tttacagcat gcctggcaaa gaatgccgtc gatgccagca
     7921 agtaccctgt tgaacaactc aacacggatg ccattgcctg gtatgccaat tatttgaaac
     7981 aatatgctgc caccaccgaa cagctttacc ttactgcggt taagggattc tatgattttc
     8041 tggctgctga ggaagctgtg gagctcaacc tgcccaaagt taaactgctc atcaagcagc
     8101 gcgcccgcag acccggtcgc cgattgccgc aatttccggc agattccatc agtcggttgc
     8161 tggattacta tgaacaaaaa gccctgccag agaaccagga acaggaaata atactacgcg
     8221 aaatgcggga taaagccttt ctggtcacac tggcggatag cgggttgcgc gtgcacgagg
     8281 catgtggatt gcggcgcggt gatatcgact ggaatgaagg aaaagccctg attatcggaa
     8341 aaggtgataa acaagccgtt gtacgcttct ccaaacgctc tctgcgagct ctgaaggctt
     8401 atttacaagc cagatccgac ctggatggtt cctccgggcg ccctcttacc tccctgcccc
     8461 tgttcgcgcg gcatgataaa ggcgctggga acaaagtgaa acccatcacg cccaccaccg
     8521 ggcgcaacat cgtgcaggaa cgtgtggcag aagccttggg cgcggatgcg gtcggtacga
     8581 tcactccgca ttcattccgg cattatttcg tcacccgcgt gctgctgagc accggcaact
     8641 tgaaactggc acaaaactta gccagacacc aaaacatcgc cgtgacgcag cgctacgcgc
     8701 acctggctga tgacgaactg gatagcggct actggcaggt ctttgaagag ta
//
LOCUS       DAOSDS010000087         8712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3007_length_8712_cov_4.220169, whole genome shotgun sequence.
ACCESSION   DAOSDS010000087 DAOSDS010000000
VERSION     DAOSDS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3007_length_8712_cov_4.220169"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1672
                     /locus_tag="PLL88_09150"
     CDS             <1..1672
                     /locus_tag="PLL88_09150"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ01773.1"
                     /translation="PWQLQRIKDWKRLYDVLAAPAFFMAQWCVNRNDLYPYWTTLEEN
                     SSFNRVQAYKDSSNDPKIPDYQSFLNRVSLFFMDTGYLEAAMDLLKELEKISRERGNK
                     EDVQISLGNQALILKTWGRLDEAMDLFKEQERIYREMGDMHGLQLTLGNQALILHARG
                     RFDEAMQMQKEAERICRRLGNKDGIQTSLSNQAMILKEWGRLEEAMELLKEQELICRE
                     LGIEKAIQVSLGNQALILQMWGKLDESMELHKKEEQICRKLGSKDGLQRSLGNQALIL
                     QMRGKLEDAMGLFKEQERICRDMGAKRDLQSALGNQALILRSWGRLDEAMQLLKEQEN
                     AYRELGDQDGLQRSLGNQALILRSWGKVDEAMQLQKEKERICRELGNQDGIQNSLGNQ
                     ALILKALGKFDEAMALFKEQERICRELNNKEALQESIGCQAQIYHAQGRSQEAMQMQK
                     EKERICRELGNLNGIQETLGDQALILQKWGKLDEAMDLLKEKERICQQLKNVYGLCLS
                     WVYQGTTCLIKGDKATGIGLLQKAYDLARQNGYQSLVIKTKGLLDRFS"
     gene            complement(1667..2377)
                     /gene="rsmG"
                     /locus_tag="PLL88_09155"
     CDS             complement(1667..2377)
                     /gene="rsmG"
                     /locus_tag="PLL88_09155"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOJ01774.1"
                     /translation="MISLADYYHNLCKGPAVPALPDKFAKYQNELLAWNSQFNLTAIR
                     SPEEIETRHFMDSLSLLPVIQQHKAHSLIDIGSGAGFPGIPLKIADPSLHVVLVESVE
                     KKAAFCRHIIQTLGLENAEVVTERAEALGQNKRYREQFDCAVARAVAALPVLLEYLLP
                     LVRLGGIAIAQKSTSVDDEVLQARNACAVLGGGEMHIEPVVIPGVDAERNLVVIEKIK
                     STPAVYPRRVGLPAKKPL"
     gene            2455..3432
                     /locus_tag="PLL88_09160"
     CDS             2455..3432
                     /locus_tag="PLL88_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01775.1"
                     /translation="MMNRIEKEQFLDWLLEEENPSVRYFTLRAILGLAQDDERVLAAR
                     RAIMQSEPVSRILALQDPAGWWNNAQSMSLPMYLGTVWQLMLLAELGADGSDERIRRA
                     IDLVFQDLQSAEGSFPHEGERFAKQYAMDLICNDAMIAYGLAGLGVGTQDERMQRTVS
                     FLTAVVNSGDYACRFNKGAECAWGVVKMLRVLSLISEGERNENIRQAIKRGAEYLLSH
                     ELVKADFPHKEGGKISEHWFRLGFPRSYQSDLLQTAFVLTALGYGKDERLQPTIAFLR
                     GKQLPEGGWALDVTWNKMIVPVTKPSKGKPDKWISWQAFYVLQESGQIQ"
     gene            3501..4076
                     /locus_tag="PLL88_09165"
     CDS             3501..4076
                     /locus_tag="PLL88_09165"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOJ01776.1"
                     /translation="MDLTNIFVGEKIRLTEMNPQEDAEKESLWTHSIDYARLRKENPV
                     RPMAAFEIRKDIEEKLKEAEQEGCSFYFAVHSRANGEELLGFLHISGIEWANGQGLLR
                     VLMGTEEIAHDHLAESLFLGLRYAFDELNLYHIYCEVPAYEIDTIKVLEKAGFVREAC
                     LREIVYRAGSYHDRIFYGMLAEEWRQRKGLA"
     gene            4073..4639
                     /locus_tag="PLL88_09170"
     CDS             4073..4639
                     /locus_tag="PLL88_09170"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOJ01777.1"
                     /translation="MKDTYLQGESIYLRATDPEKDMKFYSGWQRDALYDRFMDSMPAV
                     MYGKDQMKEWYEKQIASHLSSFMIVHKEDDIPIGFIEISGYDWHSGNAWLGIGIGERE
                     NWGKGYGTEAISILLKLVFLEWNLHRVSLAVLGYNVRAMRSYEKAGFKNEGALRGFVQ
                     RNGQRWDMYMMGILREDWEKLHSGEIEK"
     gene            4656..5273
                     /locus_tag="PLL88_09175"
     CDS             4656..5273
                     /locus_tag="PLL88_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ01778.1"
                     /translation="MQQPFKAIIFDMGGVFIRTKHRNARQELAHQYGMNIEKLTDTVF
                     LSPVSIEAEKGLITRDELLRQLMHLLGEDEKRVQQFVEKFFSGDREDTELVGYARTLR
                     PQYKLGLLSNAFSGTREWMQERFTFLELFDVSYFSAEVGMRKPESQFFQLILDALQVE
                     PSEALFVDDFFENIQGAQRLGIQTVWYRDRDAAFAYLKSLLDISE"
     gene            5358..5430
                     /locus_tag="PLL88_09180"
     tRNA            5358..5430
                     /locus_tag="PLL88_09180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5391..5393,aa:Ala,seq:cgc)
     gene            5447..5519
                     /locus_tag="PLL88_09185"
     tRNA            5447..5519
                     /locus_tag="PLL88_09185"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5480..5482,aa:Ala,seq:ggc)
     gene            5807..8263
                     /gene="leuS"
                     /locus_tag="PLL88_09190"
     CDS             5807..8263
                     /gene="leuS"
                     /locus_tag="PLL88_09190"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOJ01779.1"
                     /translation="MPVSKTPRYIPANIEPKWQRKWEADGLYHSDIDPDRKKYYALTM
                     LPYPSGNLHIGHWYAMTPSDARARFKRMQGYNVLFPMGFDSFGLPAENAAIQRNIHPK
                     KWTYDNIDRMREQMRSMGNMFDWRREAISSDPKYYRWTQWFFIQLLKNGLAYRQKSLV
                     DWCPKCNTTLAREQVIGDDRHCERCHTPVVKKELEQWFFRITRYADELLDFTGIDWPE
                     PVKLLQTNWIGRSEGASVVFHTEQGDPIEVFTTRPDTLWGATFMVLAPEHPLLEKITS
                     SEQKARVEAYVAETIRQTDIQREATDKEKTGVFTGGYAINPVNNERIPIWVADYVLMT
                     YGTGAIMAVPAHDQRDFEFARKFRLEVRVVIQPDDMPALDGATMPESVSGRGKMVNSG
                     VLNGTPADQAFRQAIQYVEQQGFGKGTVNYRLRDWLISRQRYWGAPIPVLYCEKCGVV
                     PVADEDLPVLLPDDVKWKPTGESPLKLHPTWRFAVCPQCGGSAERETDTMDTFVCSSW
                     YHLRYLSPDEEKAPFDQKEYDYWMPVDSYTGGVEHATMHLMYTRFFHKAFRDLKVVKG
                     NEPMLQLRNQGQILGPDGQRMSKSRGNVVDPDTQVKLYGADTVRAFLMFGYRWAEGGP
                     WEAGNITGVQRWLNRVWNIFLDEAETGRFDEKDVTILRRKLHQTLRAVTRDYERFEFN
                     TIVSGLMELSNYLQEARAAGAAANVVWKEVTSIYLRMLAPICPHISEEIWNQLGLPYS
                     VHAQPWPELDEKATEEEEITIVLQVNGKLRDKMVVPVDISEEKAKELALANETVQRFL
                     EGAAPRKIIYVPGKLVNIVK"
     gene            8466..>8712
                     /locus_tag="PLL88_09195"
     CDS             8466..>8712
                     /locus_tag="PLL88_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HOJ01780.1"
                     /translation="MGIPHSEIYAAILEGDEETVSAQVAKAIAIGVPAGILLNETLIP
                     AMAEVGDLFERGEYFVPEMLVAARAMKAGLAILKPLLA"
BASE COUNT         2349 a         1869 c         2465 g         2029 t
ORIGIN      
        1 gccctggcag ttgcaaagaa taaaagattg gaaaaggcta tatgatgttt tggcagcccc
       61 ggctttcttc atggctcaat ggtgtgtaaa ccgaaatgat ctatatccgt attggaccac
      121 tcttgaagaa aattcaagtt tcaatagggt tcaagcatac aaagacagca gcaacgatcc
      181 taaaattccg gactatcagt cattcctgaa tcgggtttct ttatttttta tggataccgg
      241 atatctggaa gctgctatgg atttactaaa agaactggaa aagatcagcc gggaaagagg
      301 gaacaaagag gatgttcaaa tctccctcgg caatcaagct ttgatcctga aaacatgggg
      361 cagattggat gaagcgatgg atttgtttaa ggagcaagaa cgtatctatc gcgagatggg
      421 agatatgcat ggcttgcagc ttactttagg caaccaagcg ttgatcttgc atgcaagagg
      481 cagattcgat gaagccatgc aaatgcagaa agaagcagaa cgcatttgcc ggaggttggg
      541 caataaagat ggcattcaaa cttcgttgag caatcaggcg atgatcttaa aagaatgggg
      601 cagattggaa gaggcgatgg aattattgaa ggaacaagaa ctgatctgcc gggaattggg
      661 gattgaaaaa gccatccagg tttctctggg caatcaagcg ttgatcctgc aaatgtgggg
      721 taagttagac gagtccatgg aattgcacaa aaaagaagaa cagatttgcc gaaagttagg
      781 aagcaaagat gggctacagc gttctctggg caatcaagcg ttgatcttgc aaatgcgggg
      841 gaaattggaa gatgccatgg gattgttcaa agaacaggaa cggatctgca gagacatggg
      901 ggctaaaagg gacctccaat ctgcgctggg caaccaggct ttgatcttga gatcctgggg
      961 caggttagac gaagctatgc aattgctgaa agaacaggaa aatgcctatc gggaattagg
     1021 ggatcaagat gggctacagc gttcccttgg caaccaggct ttgatcctga ggtcatgggg
     1081 caaagtggat gaagccatgc aattgcagaa agaaaaagaa cgaatttgca gagaactggg
     1141 aaatcaagat ggtatccaga attccctggg aaatcaggcc ttgatcctga aagctctcgg
     1201 aaaatttgac gaggccatgg cattgttcaa agaacaggag cgcatctgtc gtgaactgaa
     1261 caataaagaa gctcttcaag aatccattgg ttgtcaggcg cagatttacc atgcgcaggg
     1321 tagatctcag gaagctatgc aaatgcagaa agaaaaagag cgcatttgtc gggagttagg
     1381 gaatctgaac gggatccagg agaccctggg tgatcaggca ttgatcctgc aaaaatgggg
     1441 caagctggat gaagccatgg atttgctcaa ggaaaaagaa aggatttgcc agcaattaaa
     1501 gaatgtctat gggttgtgtt tatcctgggt gtatcaggga acaacctgtc tcataaaagg
     1561 tgataaagcg acaggaatag gattgctgca aaaagcatat gacctggcaa gacaaaatgg
     1621 gtatcagtct cttgtgataa aaaccaaggg acttcttgat agattttcat aaaggtttct
     1681 tggctggcaa accgacccgc cttggataca cggctggagt ggactttatc ttctcaatga
     1741 cgaccaggtt gcgttcggca tccacacccg gaatcaccac cggttcgata tgcatctccc
     1801 cgccacccag aacggcgcag gcgtttctgg cttggagcac ctcatcatcc acagatgtgc
     1861 tcttttgagc gatggcgatc cctcccaagc gtaccaacgg cagcagatat tccagcagca
     1921 ccggcagggc tgccacggcg cgcgcgaccg cgcaatcgaa ctgctcgcga tagcgcttgt
     1981 tttgcccaag cgcttctgcc cgctccgtca ccacttccgc attttcaaga cccaatgttt
     2041 ggatgatgtg ccggcagaag gcagcttttt tctccactga ttccaccaac accacgtgca
     2101 aagatgggtc ggcgatcttg agcggaatgc ccggaaagcc ggctccgctg ccgatatcga
     2161 tcagagaatg agctttgtgt tgctgtatca cgggcagcag gctgagcgaa tccatgaagt
     2221 gcctggtttc gatctcttcg ggcgagcgga tggcagtcag attgaactgg gaattccacg
     2281 ccagcaattc attctgatat ttggcaaatt tatccggcaa agcaggaact gcgggtcctt
     2341 tgcacaggtt atggtaataa tctgccagac tgatcatatt cccattataa acatagataa
     2401 ccggacagtt ggaattttca tgattaatga atatgattaa gcatagcgga gaaaatgatg
     2461 aacaggatag aaaaagaaca attcttggac tggttgctgg aagaagagaa tcccagtgtg
     2521 cgctatttca ccctgcgggc tattctgggc ctggcgcagg atgacgagcg ggtgcttgcc
     2581 gcgcgccgtg ccattatgca aagtgaaccg gtgtcgcgca ttttggcatt gcaggatcct
     2641 gcaggctggt ggaataacgc acaatccatg agtttgccca tgtatttggg caccgtttgg
     2701 cagttgatgc tgctggcgga attgggcgcc gatggcagcg atgaacgcat ccgcagagcc
     2761 attgatctgg tgtttcagga tctgcaaagc gcggaagggt ccttcccgca tgagggtgaa
     2821 cgttttgcca aacagtatgc catggatctg atctgcaacg acgccatgat cgcctatggt
     2881 ctggcggggt tgggtgtagg cacacaggat gagcgtatgc agcgcactgt atctttcttg
     2941 accgctgttg tgaacagtgg agattatgcc tgccgtttca ataaaggagc agaatgcgcc
     3001 tggggtgtgg tgaaaatgtt gcgtgttctg tctctcattt ctgaaggtga acggaatgag
     3061 aacattcggc aagcgatcaa acggggtgct gaatatttgt tgtctcatga actggtgaag
     3121 gctgattttc cccacaagga agggggaaaa atcagcgaac actggttccg cctgggcttt
     3181 ccacgcagtt atcaatctga tctgttgcaa acagcctttg tgttgacagc cctgggttat
     3241 ggaaaagatg agcggctgca gccaaccatc gcttttctgc gcggtaaaca actgccagag
     3301 ggcggctggg cgctcgatgt gacctggaac aagatgatcg tacctgtcac caaaccatcc
     3361 aaaggaaaac ctgataagtg gatcagttgg caggcgtttt atgtgctgca ggaaagcgga
     3421 cagatccaat aaccagattc cgggcaacag tttctgataa aattcaaggg tttcattata
     3481 aatagaacaa agggtagaaa atggatttaa ccaatatttt tgtgggtgag aaaattcggc
     3541 ttaccgaaat gaacccccaa gaggatgctg aaaaagaaag cctgtggact cattccattg
     3601 actacgcacg cctgcgcaaa gagaatccgg tgcgccctat ggctgctttt gagatcagga
     3661 aagatattga agaaaaatta aaagaagcag agcaggaagg atgtagtttc tattttgccg
     3721 ttcattccag ggcaaatggg gaagaactgc tgggtttttt gcatatcagt gggattgaat
     3781 gggcaaatgg acagggactg ctgcgggttc tgatgggaac tgaagagatc gcgcatgatc
     3841 atctggcaga aagcctgttt ctgggcttgc ggtacgcttt tgatgaattg aatttgtatc
     3901 atatttactg tgaggttcct gcttacgaaa tagacactat caaggtgctt gaaaaagccg
     3961 gcttcgtgcg cgaggcgtgt ttgcgcgaga ttgtctaccg tgcggggagc tatcatgacc
     4021 gcattttcta tggaatgctc gcagaggagt ggcgacaaag gaaagggctg gcatgaaaga
     4081 tacgtatcta caaggtgagt cgatctacct gcgtgcgacg gatcccgaaa aggatatgaa
     4141 gttctattcc ggttggcagc gagacgccct gtatgaccgt tttatggata gtatgcctgc
     4201 ggtgatgtac ggcaaagacc agatgaaaga atggtatgag aaacagattg ccagccattt
     4261 gagctctttc atgatcgtcc acaaagaaga tgatatcccg atcggtttta ttgaaatatc
     4321 gggttatgat tggcacagtg gaaatgcatg gttgggaatc gggattggcg agcgcgaaaa
     4381 ttggggcaag ggatatggta ccgaagccat cagcattctg ctgaaactgg tgttcctgga
     4441 atggaatctg caccgggtct cgctggctgt gctcggctac aacgtgcgcg ccatgcgctc
     4501 ctatgaaaaa gcggggttca aaaatgaagg cgcgctgcgt ggattcgtcc agcggaatgg
     4561 acagcgctgg gatatgtaca tgatgggcat cctgcgggaa gattgggaaa aactccattc
     4621 tggagaaatt gaaaaataaa taaggaagag aaaacatgca acaaccattc aaagcgatca
     4681 tcttcgatat gggcggtgtt tttatccgta caaaacatcg aaatgcccga caggaattgg
     4741 ctcaccaata tggaatgaac atcgaaaaat tgacggatac ggtttttctg agcccggtca
     4801 gtattgaagc agaaaaaggg ttgatcacca gagatgaatt gcttcgtcaa cttatgcact
     4861 tgctcgggga ggatgaaaaa agggttcagc agtttgtaga aaaattcttc agcggtgatc
     4921 gagaggatac ggaattggta ggttatgcgc ggactttgcg tccgcagtat aagctgggtt
     4981 tgttgagcaa tgcattttca gggaccagag aatggatgca ggagcgtttc acgtttcttg
     5041 agttgtttga tgttagctat ttttcagcag aggtgggcat gcggaaaccg gaatcacagt
     5101 tcttccaatt gatcctggat gcgctgcagg ttgaaccttc tgaagccctg ttcgtagatg
     5161 attttttcga aaatattcag ggcgcacaaa ggttgggcat ccaaacagtg tggtatcgcg
     5221 atcgagacgc agcttttgct tatttaaagt cgcttctcga tatctctgaa tgagaaacgc
     5281 catctatgat atactctgca tatgctatcg cttgacagct tgatagctct tgggtagaat
     5341 tttcctaccc tgaaattggg cctgtagcgc agttgggagc gcgcttcaat cgcactgaag
     5401 aggtcgagag ttcgaatctc tccaggtcca tctgggcaag gcaatggggc ccatagcgca
     5461 gttgggagcg cgtctcaatg gcattgagaa ggtcgagagt tcgaatctct ctgggtccac
     5521 tgaacagaaa atgaaaaagt gaaaagagga gtagtatttc atccagcagc gattcgggag
     5581 agtgaggtgt gagcccgata cggcgcccat ggagcgtaga aaaggaagaa ctcgcctctt
     5641 gagcggaaac gctgcactgt ggcagtgaaa tgaagtagct gctaccggat aagcccgtta
     5701 gagctgcctg agaggttcgc aagcgaacaa tcagggtggt accacggaga aacccttcgt
     5761 ccctgaggat gaaggattct ttttttaaca aagaaaggaa gtgcgcatgc cagtatcaaa
     5821 aacaccacgc tatattccgg caaacatcga accgaagtgg caaagaaaat gggaagcaga
     5881 tgggttgtat cattccgata tcgatcccga tcggaagaaa tactatgcac tcaccatgct
     5941 tccataccct tcgggaaatt tgcacatcgg tcactggtat gccatgacac cctcggatgc
     6001 ccgcgcgcgc ttcaagcgca tgcaggggta taacgtgttg ttccccatgg gttttgactc
     6061 cttcgggctg ccagcagaaa acgctgccat ccagcgcaat attcatccaa aaaaatggac
     6121 gtatgacaat atcgatcgca tgcgcgagca gatgcgcagc atgggcaata tgttcgactg
     6181 gcggcgcgaa gccatttctt cagatcccaa gtactaccgc tggacgcagt ggttcttcat
     6241 tcaattgtta aagaacggat tggcttaccg gcagaaatca ctggtagatt ggtgccccaa
     6301 gtgcaacacc accctggcgc gagaacaggt gataggagat gatcgccact gcgagcgctg
     6361 ccacacaccg gtggtgaaga aagaactgga gcagtggttc ttccgcatca cccgctatgc
     6421 tgatgagttg ctcgatttta ccggcattga ttggcccgaa ccggtgaaac tattgcaaac
     6481 caattggatc gggcgatcag aaggcgcttc tgtcgtcttt cacaccgagc agggcgatcc
     6541 gattgaggtg ttcacgaccc gcccggatac tctgtggggt gcgaccttta tggtgctggc
     6601 tccggagcac ccactgctcg aaaagatcac cagctccgaa cagaaagcca gggtggaagc
     6661 ctatgttgca gaaacgatca ggcaaactga catccaacgg gaagccactg ataaagaaaa
     6721 gacgggtgtt ttcaccggcg ggtatgccat caatccggtg aacaatgagc gcatcccgat
     6781 ttgggtggca gattatgtgt tgatgaccta cggaacaggt gccatcatgg cggtgccggc
     6841 gcatgatcag cgcgatttcg aattcgcacg caagttcaga ttggaggtgc gggttgtgat
     6901 ccaaccagat gacatgcccg cgctggatgg ggctaccatg ccagaatctg tttcggggcg
     6961 cggaaagatg gtcaattcag gtgtgttgaa cggaacgccg gcagaccagg cgttcaggca
     7021 ggctatccag tatgttgaac aacaggggtt tggcaaaggt actgtcaatt accgcctgcg
     7081 cgattggttg atctcacgcc agcgctactg gggcgcaccc attccagtgc tgtattgtga
     7141 gaagtgcggg gtggttccag tggcggatga ggatctgccg gtgctgttgc cggacgatgt
     7201 gaaatggaag ccgaccggcg agagcccgct caaactgcat cccacctggc gctttgccgt
     7261 ctgcccgcaa tgcggaggca gcgctgagcg cgaaacggat accatggata ccttcgtgtg
     7321 ctcctcctgg tatcatctgc gctacctgag cccggatgag gaaaaagccc ccttcgacca
     7381 gaaagagtat gattactgga tgccggtgga tagttatacg ggcggtgtag aacatgccac
     7441 catgcatttg atgtacacgc gtttctttca taaagccttc cgtgatctga aagtggtgaa
     7501 agggaacgag cctatgctgc aactgcgcaa ccagggtcag atattgggac cggacgggca
     7561 gcgtatgagc aaatcacgcg gcaatgtggt ggatccggac acacaggtga aactgtacgg
     7621 agcggatacg gtgcgcgcct tcttgatgtt tggttaccgc tgggcagaag gtggtccatg
     7681 ggaagcgggc aatatcaccg gggtgcagcg ctggctgaac cgcgtctgga acatctttct
     7741 ggatgaagca gaaacgggcc gttttgatga aaaagatgtt actattctgc ggcgcaaact
     7801 gcaccaaacg ctcagggctg tgaccaggga ttatgaacgc tttgagttta acaccatcgt
     7861 ttcagggttg atggaactct cgaactattt gcaggaagcg cgtgcagcgg gcgcggctgc
     7921 gaatgtagtt tggaaggaag tgaccagcat ctacttgcgc atgctggcac ccatttgtcc
     7981 gcatatttct gaggagattt ggaatcaact ggggctgcca tattccgtcc atgcccagcc
     8041 gtggcccgag ctggatgaga aagccaccga ggaagaggaa atcaccatcg tgctacaggt
     8101 gaacggtaaa ctgcgcgata agatggtggt gccggtggac atcagcgagg agaaggcgaa
     8161 ggagttggca ctggcgaatg aaaccgtgca gcgttttcta gagggggctg ccccgcgaaa
     8221 aatcatctat gtgccgggaa aattggtgaa catcgtaaaa taagctgaaa taacctgtgc
     8281 aggcgctttc tttggaggcg cctgcttttt gttttctttg cactgagaaa aatcgacttt
     8341 cacattattg caagagaagc gcttgacttc ttctgggcaa atccctataa tacaatagtg
     8401 aataaaattg gcataatata ttcacgatag tgaattttca atcatcaatg gacgcgggag
     8461 aaacaatggg aattcctcat agcgaaatct atgcagccat tttggagggt gatgaagaaa
     8521 cagtatccgc gcaagttgca aaagcgatag ccataggggt acccgccgga attctcttga
     8581 acgaaacgtt gataccggct atggcagaag tgggcgatct gtttgaaaga ggagaatact
     8641 ttgtgcccga gatgctggtg gcggcgcgtg ccatgaaagc cgggctggcg atattaaaac
     8701 cattattggc gg
//
LOCUS       DAOSDS010000088         8442 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3135_length_8442_cov_4.880410, whole genome shotgun sequence.
ACCESSION   DAOSDS010000088 DAOSDS010000000
VERSION     DAOSDS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3135_length_8442_cov_4.880410"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLL88_09200"
     CDS             <1..318
                     /locus_tag="PLL88_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01781.1"
                     /translation="GTLLQIILQSVTYKTLLWMAVVAAGLFCIGFLILFFNKPKTKRE
                     TADIVEEEISYQEADEEMLEAKQAMAEAVSVEPTTDEEVPEEEEKKKRSKKASRKTSQ
                     EEE"
     gene            complement(332..2893)
                     /gene="mutS"
                     /locus_tag="PLL88_09205"
     CDS             complement(332..2893)
                     /gene="mutS"
                     /locus_tag="PLL88_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ01782.1"
                     /translation="MSGSDITPIRQQYLDIKRQYPDAILFFRLGDFYETFDHDAEITS
                     RELDLVLTSRSVSKGNRIPMAGIPYHAAENYIARLINKGYHVAICEQIGEQPKRGLFP
                     REVVRIITPGTLVESSFLKADKNNYLAAVYPLAESTGLAYIDITTGEFYCTELSDGLT
                     NLLHSELARINPAELLLPKSFSDERLDKYHRTLKEDWYFAFTRAQDALQHCFQVATMD
                     GFGLKDRPAAISAAGAIIQYLDETDPLSVKTIKRIRYYHTQDTMILDPETRRNLELTE
                     TIRNGQEEGSLLSVIDFTVNPLGKRLLRNWLNKPLIDPQKIHSRLEHVSYFVQDGLLR
                     KELQAHLKKVSDLERISTRIVAGHALPRDLVALRNSLELIPPIISKLAEVPEPIKTLV
                     SRIHACPEILTLLTSAIADDPPNTLQNIGIIKKGYSSELDTIYTASHDAKQWIANLEK
                     VEKERTGIKSLKVGYNKVFGYYIEISNSYVDQAPPEYIRKQTLVNGERFITPEMKEYE
                     SIVLNAEERIHQLELRLFKEICQKITAFVEDLSSTAQALAELDVFIGLADAAVNNRYC
                     KPVITDEKVVQIIEGRHPVVERTQLSRAFIPNDLSFDEKEFIKIITGPNMSGKSTFLR
                     QVALIILLAQIGSYVPADSATIGVVDRIFTRIGAQDEIHAGQSTFMVEMTETANILNN
                     ASSRSLLILDEIGRGTSTYDGLSIAWAVLEYVHNHPDLRSRTLFATHYHELTQLGETL
                     PHAANYNVAVSETDGSVVFLYKIIEGGTDRSYGIHVAQLAGLPRNVITRSFEILRQLE
                     EENAHTRAGIGVSDQLPLFPQTDPLKHALQELDLNQLSPIEALNLLYEWKKKYTK"
     gene            complement(2912..3679)
                     /locus_tag="PLL88_09210"
     CDS             complement(2912..3679)
                     /locus_tag="PLL88_09210"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOJ01783.1"
                     /translation="MEIPFLKQAASKYTIETDVFEGPLDLLLQLIEKEELDITQLSLA
                     KVTDQYLEHLSLIANRDPLEVSAFLVIAAQLVLIKSRILLPSETSEVDGLEIEGAVDL
                     VQQLIEYKKYKNAGFWLQDRETRHMHTYFRTAPPPKLPEVLDLTDLSVFDLAALYRDL
                     LITKEQFYPIDEVVTITTITLKNRINTIMEQFKRISHLKFSSLTDQGTKLEVIITFLA
                     LLELIKNHLVQAIQPEIFGEIDLDSIGEMDQEIETEF"
     gene            3781..4488
                     /gene="rnc"
                     /locus_tag="PLL88_09215"
     CDS             3781..4488
                     /gene="rnc"
                     /locus_tag="PLL88_09215"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOJ01784.1"
                     /translation="MSSEFVPELPQELEKRLGLKFNNVLLLIRALTHRSYLNENPEAI
                     EDNERLEFLGDAVLDFLVGEWLYDKYPEMPEGDLTQMRSALVQTEELADFARYLNLGN
                     ALRLGKGEIKTGGRIRSSLLCDAFEALIGAIYLDQGLPAIRLFLNPLLEVAVEDILEM
                     HKNEDPKSKLQEWSQSLGYPPPKYITLKASGPDHSKLFEVEVYINKKSYSKGSGKSKQ
                     SAEKDAAKKALERIYQE"
     gene            4496..8095
                     /gene="smc"
                     /locus_tag="PLL88_09220"
     CDS             4496..8095
                     /gene="smc"
                     /locus_tag="PLL88_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOJ01785.1"
                     /translation="MTQKLEALELHGYKTFASRTPFEFPDKIIAIVGPNGSGKSNIAD
                     SIRWVLGEQAFSVLRGKKTEDMIYSGSAQRSKMSMASATIRFNNEDGWLPIDYDVVSI
                     TRRAYRSGENEYLLNDKKVRLRDIQELLSNSGLAERSYTIIGQGLVDSALSLKPQDRR
                     LFFEEAAGIRLYRARREEAIRKLDKTVENMERVKDILFEIRPRIARLEKQVERAKEYN
                     RINADLRLLLKDWYGYRWNLLQQDYRESLDARMKKDAELNSIRMNVMGADEEMANRRK
                     VLQEKRNTLSIWNNQLAELQSEKEKINRELAIIDERFKGYEEQRDSLRSSMTYAEEEL
                     RGRQEQKHDLESKHAEQEKNLETMQHAVDALEASLNQKKSELQQVQEQIAELQQKKLD
                     IDKTIVGNQAKITDLETRAEILIKRRTELDESIKNQETDYQEILTSVAQAQDLLNEKQ
                     GKMNQDLDELRYLNEQKEKIDAEITRIRNDVQQYSHELVAKETQFNVLVTAKKNMEGF
                     KTGTKKFLHTIQQRKVRGTFELLLSCMRVEAGYEKAVSAALGDATEGLILDKSLELQN
                     ILSLMRDAGDSHSILIPQSLIKEKRSAPRSVSGCRPLLDFVHSEPEYRSVLQILFEDT
                     YILEDPKELEQILTKLEKDQKIVLKDGGIFYGNGLIAVGGVTSEFIFRREKEIEDLRD
                     AMNDLRGKIAEVDKSEKGQKKDLEELEKKIIELREKISLSEKDVKDVQSTLKDIQTKQ
                     ADASQDIRLNKQLVAKLLVDIQDAIEAKTGLKQATKNAQLQTETLNQEIEKLKKQASA
                     ISLEELNQGYFQKKTEFAVAQESYQSSSSRLQDIIQLIAGLESQCNDQKEKLTSLETQ
                     HTELKEKYTLDTHRLDELNVQIDALMEKIDPVENEITTITTEIEKLQNSNEDLRKNQS
                     IIERHFIQLEMQVDKVKNQMDVLKDRISDDFGLVVYEYEPDISGPKPLPIDGMVANLP
                     KVTQIPDDLEDQIKNLKSAIHRMGAVNPEAEKEFREESERYEFLTAQMTDLEKAESDL
                     RKVVVELEDIIKIKFKETFKAVNEEFSDIFKQLFGGGSARLVIEDEENITETGIEIEA
                     TLPGKRKQELALLSGGERSLTAIALIFALLKISPTPFCVLDEVDAMLDESNVLRLGQL
                     MKELSDKTQFIVITHNRNTVQLANVIYGVTMGRDSASQVISLKLDEVTDDLVD"
     gene            complement(8148..8442)
                     /locus_tag="PLL88_09225"
                     /pseudo
     CDS             complement(8148..8442)
                     /locus_tag="PLL88_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611963.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2467 a         1742 c         2129 g         2104 t
ORIGIN      
        1 ggaacgttgt tgcagattat tttgcagtca gtcacttaca aaacattatt atggatggct
       61 gtggtcgcgg ctgggttgtt ctgcattgga tttctcatcc tgttcttcaa caagccgaaa
      121 acaaaaagag agaccgcaga tatagttgaa gaagaaatat cctatcaaga agcagatgaa
      181 gagatgctgg aagctaagca agccatggct gaggctgtct ctgtcgaacc aacgaccgat
      241 gaagaagtac ctgaagaaga agagaaaaag aaacgctcca aaaaagccag ccgaaaaacg
      301 tcacaggaag aggagtgatc gctcctcttc cttatttcgt gtacttcttt ttccactcgt
      361 aaagaaggtt caatgcttca atcggtgata gctggtttag atccaattct tgcaatgcgt
      421 gtttcagtgg atcggtttgg gggaataatg gcaactggtc ggataccccg atccctgccc
      481 gggtatgagc attctcttct tcgagttgtc tcaaaatctc gaacgagcgc gtgatgacgt
      541 ttcgcggaag cccggctaac tgagcgacat gaatgccgta tgatcggtcg gtaccgcctt
      601 cgatgatctt gtataaaaat actacgcttc catctgtttc ggagactgcc acattatagt
      661 tggcggcatg cggtaaggtc tcgcccagtt gggtcaattc atgatagtgg gtggcaaaca
      721 gggtgcgcga gcgcaggtcg gggtgattat gaacatattc cagaaccgcc cacgcgatgg
      781 aaagcccatc gtaggtgctg gttcctcgtc caatctcatc cagaatcaac aaactgcgtg
      841 aagaagcgtt gttcaggata ttggctgttt cggtcatttc gaccatgaaa gtggattggc
      901 cagcgtgaat ctcgtcttgc gcgccgatgc gggtgaaaat tcggtcaacc accccaatag
      961 tggcggaatc agccggtaca tagctgccaa tctgcgcaag caagatgatc agtgccacct
     1021 ggcgcagaaa ggtgcttttt ccactcatgt tgggaccggt gattatctta atgaactctt
     1081 tttcatcgaa agaaagatca ttgggtatga aagctctgga aagctgcgtc ctttctacca
     1141 ccggatgcct gccttcgatg atctgcacga ccttctcatc ggtgatgaca ggtttgcagt
     1201 agcgattgtt taccgcggca tctgccagac cgataaacac atccagctca gccaatgcct
     1261 gcgctgtcga tgataggtct tcgacaaatg cggtaatctt ctgacagatc tctttgaata
     1321 agcgcaattc aagctggtga atgcgttctt ccgcgttcag gacgatcgat tcatattctt
     1381 tcatttcggg ggtgataaaa cgctcaccat tcaccagcgt ttgcttgcgt atgtattcgg
     1441 gaggggcttg atctacataa ctattggata tctcgatata gtatccgaac accttgttat
     1501 atccgacttt gagcgacttg atcccggtgc gctctttttc aaccttttcc aggttggcga
     1561 tccactgttt ggcatcatgg gaagcagtgt aaattgtatc aagctcggag gaatagccct
     1621 ttttaatgat gccgatgttc tgcagggtat tgggcgggtc atctgcaata gctgatgtta
     1681 gtaatgtcaa tatctctgga caggcgtgga tacgagaaac caatgtcttg atcggttctg
     1741 gcacttcggc taattttgaa atgataggag ggatcagttc cagtgaatta cgtagcgcta
     1801 ccagatcgcg tggcaaggcg tgcccggcta caatacgggt ggagatcctc tccaggtctg
     1861 agactttttt cagatgagct tgaagctctt tgcgcaataa accgtcttgg acaaagtaac
     1921 taacgtgctc gagacgagaa tggatctttt gtgggtcgat caaaggtttg ttcaaccaat
     1981 tgcgcaacaa tcgtttgccg agcggattca ccgtgaaatc gatgacggaa agcagagaac
     2041 cttcttcctg accgttgcga atggtctccg tgagttccag gttgcgccgc gtttcgggat
     2101 cgaggatcat ggtatcttgg gtgtggtaat agcggattct cttgatcgtc ttcacagata
     2161 acggatcggt ttcatcgagg tattgaatga tcgcgccggc ggccgatatg gcggcagggc
     2221 ggtctttcag accaaagcca tccattgtgg cgacctggaa gcaatgctgt aaggcgtctt
     2281 gtgcgcgggt aaaggcaaag taccagtctt ctttgagcgt gcgatgatat ttatcgagcc
     2341 gctcgtcgga aaagcttttg ggcaacagca gttcagccgg gttgatgcga gccagctctg
     2401 aatgcaacaa attggtaaga ccatcgctga gttctgtgca ataaaattca ccggtggtaa
     2461 tatcgatata agccaaccct gtactctctg cgagtggata gaccgctgca aggtaattgt
     2521 tcttatcagc ttttagaaag gatgattcaa ccagcgttcc cggagtgatg atgcgcacca
     2581 cttcacgagg gaacaatccg cgtttgggtt gctctccaat ctgttcacaa atggctacgt
     2641 ggtagccttt gttgatgagt cgggcaatgt aattctcagc ggcatgatag gggatacctg
     2701 ccatggggat gcggtttcct ttggaaacac tgcgagaggt cagcaccaga tcaagttctc
     2761 gagaggtgat ttcagcatcg tggtcgaacg tttcgtaaaa atcacccagc cggaagaaga
     2821 gaatggcatc gggatactgc ctcttaatat caagatattg ttgccttatg ggtgtaatat
     2881 ctgaaccgct catgcttctt ccccgctgcg cctagaattc cgtttcgatc tcttgatcca
     2941 tttctccgat ggaatcgaga tcaatctcgc caaatatttc gggctgaatg gcttgaacca
     3001 aatggttttt gatcagttct aacaaagcca aaaaagtgat gatgacctcc agtttggtgc
     3061 cctggtcggt caaggatgaa aactttaaat gggatatcct tttaaactgc tccatgatgg
     3121 tgttgatgcg gttcttcaat gtgatggtcg tgatggtgac gacctcatct atcggataga
     3181 attgttcttt tgtgatgaga agatctctat acaaagctgc cagatcgaaa actgacaggt
     3241 cggtaagatc aagcacctct ggcagtttgg gagggggagc cgtgcggaag taagtatgca
     3301 tgtgcctggt ttcgcggtct tgcaaccaaa aaccagcgtt cttatacttt ttgtattcga
     3361 tcaactgctg taccaagtca acagcgcctt caatctccaa accatcaacc tcggaggttt
     3421 ctgatggcaa taggatacga gatttgatca gcaccagttg tgcagcgatc accaaaaatg
     3481 ctgagacctc caagggatca cggttggcga tcaaactgag gtgttcgagg tattgatcgg
     3541 ttacctttgc cagcgataac tgtgtgatat ccagttcttc tttttcaatc agttggagca
     3601 acaaatcaag cggaccttca aatacatctg tctcaatggt atatttactg gctgcttgct
     3661 tgagaaaagg aatttccatg gagtacatta tagcaggggg tttccgattc gcaatagtga
     3721 ttgtgaaata gtaaaataag aaaacgtaag tatgcattta ttctcggagg aaaattgaaa
     3781 gtgtcatcag aattcgttcc tgaacttccg caagaattgg aaaaacgcct tggattaaag
     3841 ttcaataatg ttcttttgtt aatacgggca ctcacgcacc gatcatatct caacgaaaac
     3901 cctgaggcga tcgaggataa tgaacgtctt gaatttttag gtgatgccgt gcttgatttt
     3961 ctggttggag agtggttata tgataagtat ccagaaatgc ctgaaggtga tttaacccag
     4021 atgcggtcag ccctcgtcca aacagaagaa ctggcagatt ttgctcgtta tttaaatttg
     4081 ggaaatgcgc tacgacttgg caaaggggaa attaaaactg gggggagaat cagaagcagc
     4141 ctgttgtgcg atgctttcga agcgctgatt ggtgcgattt accttgacca ggggttacca
     4201 gccattcgtt tgttcttaaa tccgctcctg gaagtggctg tagaggatat tttggaaatg
     4261 cacaagaatg aagatccgaa atcgaaattg caggaatggt ctcaatcgct tggctatcct
     4321 cccccaaaat atattacgct aaaggcatct ggtccggatc attcaaaatt gtttgaagtg
     4381 gaagtgtata ttaataagaa gtcatactcc aaaggaagcg gaaagagcaa acaatctgca
     4441 gaaaaagatg ctgcaaaaaa agcactagag aggatctatc aggaataatc accgcatgac
     4501 tcaaaaatta gaagcgttag aactccatgg gtataaaacc tttgcctcca ggactccatt
     4561 tgaattcccg gacaagatta ttgctatcgt tggaccgaac ggatcaggaa agtcaaatat
     4621 cgctgattcc atacgctggg tattaggcga acaggctttc agtgtgctgc gcggcaagaa
     4681 aaccgaagac atgatctact cgggctccgc tcagcgttct aaaatgtcca tggcgtctgc
     4741 cacgatccgc ttcaataatg aagatggttg gctgccaatc gattatgatg tggtgagcat
     4801 cacgcggcgg gcatatcgaa gtggagaaaa tgaatatctg ctcaacgata agaaagtgcg
     4861 cctgcgagat atacaggaac ttttatccaa ttcaggcctg gcagaaagaa gctacaccat
     4921 cataggacag ggattggttg attcagccct ttcactgaaa ccacaagacc gcaggctctt
     4981 tttcgaagag gcggctggga tccgcttgta cagagcgcgc cgagaagaag cgatccgaaa
     5041 actggataag acggttgaaa atatggagcg cgtgaaagat atcctatttg aaattcgccc
     5101 gcgcattgca cgtttagaaa agcaggttga acgtgccaag gaatacaacc gcatcaatgc
     5161 tgacctgcgc ttactattaa aagattggta cggatatcgc tggaatttgc tgcagcaaga
     5221 ttatagggaa tcgctggacg ccagaatgaa aaaggacgcc gaactgaaca gcatccgaat
     5281 gaacgtgatg ggcgctgatg aagagatggc aaatcgaaga aaagttctgc aggagaaaag
     5341 aaataccctt tccatatgga acaatcaact tgccgaactg caaagcgaaa aagaaaagat
     5401 caatcgcgaa cttgccatta tcgatgaacg cttcaaaggg tatgaagagc agcgtgattc
     5461 gttacgttca tccatgacct atgccgagga ggaattgaga ggtcgccaag aacaaaagca
     5521 tgacctcgaa tcaaagcatg cagaacaaga gaaaaatctt gaaactatgc agcatgcagt
     5581 agacgcgctg gaggcatctt taaatcagaa gaagagcgaa ctgcagcaag ttcaggagca
     5641 gattgcagaa ctgcaacaga agaaactgga tatcgacaaa accattgtgg gaaatcaggc
     5701 aaagatcact gacctggaaa cccgagcgga gatcctgatc aaacggcgta ccgagttgga
     5761 tgagtcgatc aaaaaccagg aaactgatta ccaggagatc ctgacatcgg ttgcgcaggc
     5821 tcaagacctg ctcaatgaga aacaaggaaa aatgaaccag gacctggatg agttgcgcta
     5881 tctgaatgag caaaaagaaa agatcgatgc tgaaattacc aggatcagga atgatgtcca
     5941 acagtattcc cacgaactcg tggcgaaaga gacccaattc aacgtcttgg ttaccgccaa
     6001 gaagaacatg gagggtttca aaactggaac caagaaattc ttgcatacca ttcagcaaag
     6061 aaaagtgcgg ggaacgtttg aattgttgct atcctgtatg cgggttgaag caggctatga
     6121 gaaagcggta tccgcagcgc ttggagatgc cacagaagga ttgatcctgg ataaatcgct
     6181 ggagcttcag aacattctgt cattgatgcg cgatgcgggg gattctcatt cgattttaat
     6241 tccacaatcg ctgataaaag agaaaagaag tgcgcccagg agtgtaagtg gatgccgacc
     6301 tttgttggat tttgtacatt cagaacccga ataccggtca gtcttacaga tcttgtttga
     6361 ggatacctat attcttgaag accccaagga gctcgaacaa attctgacga aactggagaa
     6421 ggatcagaag attgttctga aagacggcgg tatcttttat ggaaatggcc tgatcgctgt
     6481 gggtggcgtt accagtgagt tcatttttag aagagaaaaa gagatcgaag acctacgaga
     6541 cgcgatgaat gacctgcgtg gcaagatcgc ggaggtcgat aagtctgaaa aaggtcagaa
     6601 aaaggatctt gaagagctcg aaaagaagat catcgagttg cgcgaaaaga tttcactatc
     6661 agaaaaagat gtgaaagatg tccaatcgac attaaaggat atccaaacca agcaggcgga
     6721 tgcgtcacaa gatattcgat tgaataaaca attggtcgct aagttgcttg tggatattca
     6781 agatgccatc gaagcgaaga ccggattgaa acaagcgact aagaatgcac agcttcagac
     6841 cgaaaccttg aaccaggaaa tcgagaagct gaagaaacag gcatcagcca tttcattgga
     6901 ggaactgaat cagggctatt tccaaaagaa aacagagttt gcggtggcac aggagagcta
     6961 tcagagctcc agcagccggt tgcaagatat tatccaactc attgctggtt tggaatctca
     7021 atgcaatgac cagaaggaaa agttgacttc tcttgaaacc caacataccg agttaaaaga
     7081 aaagtatacc ctcgacaccc atcggcttga tgaactcaat gtgcagatcg acgcgttaat
     7141 ggaaaagatc gatccagttg aaaatgaaat tacaacgatt actacagaga tcgaaaaact
     7201 gcagaactca aacgaggacc tgcgaaagaa ccaaagcatt attgaacggc attttatcca
     7261 actggaaatg caggttgata aagtcaagaa ccaaatggat gtgttaaaag accgcatctc
     7321 tgatgatttc ggtctggttg tatatgaata cgaacctgat atcagcggtc caaaaccgtt
     7381 gcccattgat ggtatggttg ccaatcttcc aaaagtgacg caaattcccg atgatctgga
     7441 agatcagatc aagaatctga aatctgctat ccatcgtatg ggagccgtaa atcctgaagc
     7501 ggaaaaagaa ttccgcgaag aaagcgaacg gtatgagttc ctgacagcgc aaatgaccga
     7561 tctggagaag gcagaaagtg acttgcgcaa ggttgtagta gaactcgagg acatcattaa
     7621 gatcaagttc aaagaaacct tcaaagctgt gaatgaggag ttttcagaca tcttcaagca
     7681 attgtttgga gggggaagcg caagactggt tatcgaagat gaagagaata tcaccgagac
     7741 cggcattgaa atcgaagcca ccttaccagg aaagcgaaaa caagagttag ctttgttgtc
     7801 aggtggagaa agaagcctga ccgcgatcgc gttgatcttt gcgctattga agatttctcc
     7861 cacaccgttc tgtgttctgg atgaagtgga tgcaatgctg gatgaatcca atgtattgcg
     7921 attgggtcaa ttaatgaaag agttgagtga taaaacccaa ttcattgtga ttacgcacaa
     7981 tcgaaacacg gtccagctcg ctaatgtcat ctatggagtt accatggggc gcgattcagc
     8041 cagccaggtg atcagtttga aattggatga agtgacggat gatctggtcg attagaaaac
     8101 ataaaaaaag agctggtttg tagtgcaccc caattattgg acagaagtta agtagagatg
     8161 aaggactgag tacgaaattg aacagggctc agtcctttta gtttagcctt gattctgcgc
     8221 ttattgtagt aatcaatgta ctcagataac gctttttcaa agtgctgaaa agaggaaaag
     8281 ttttggttgt ggtagaactc cgacttgaga ataccaaaga agttttccat gacactgttg
     8341 tcatagcagt ttcccttcct ggacatgctc tggcggatgc ccttgtctga tagctgttca
     8401 tctgatactg ccagccctgg tctgaatgca ggatcaactg gc
//
LOCUS       DAOSDS010000089         8310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3200_length_8310_cov_4.674985, whole genome shotgun sequence.
ACCESSION   DAOSDS010000089 DAOSDS010000000
VERSION     DAOSDS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3200_length_8310_cov_4.674985"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1922)
                     /locus_tag="PLL88_09230"
     CDS             complement(273..1922)
                     /locus_tag="PLL88_09230"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01786.1"
                     /translation="MTKSDNKQRKTRDFIKNVLGEVPLTAELYWLIRNRSKGIQSRFS
                     LTDLDKHIPEIVQYLCANQTAAEPVKKILVFATLHYWISHAALISAALTGLGYDVSLG
                     YLPYHDWQNPINRFDLRRQNLYAQSILKKLNPFVKTFSFLNVYAGYKTLPAELAQKAE
                     QVARFDSQYTLQVETVDTDEPVYKMRLERNEDIARNALAWLTTNKPDVVIIPNGTIQE
                     FAVVYEVAKYLNIPVTTYEFGDQRQRVWIAQDAKVMEQETDALWEAYRDKPLERSELH
                     KTRELFEARRKASIWKNFSRLWQRAPAKGVTAARKELGLDDERPVVLLATNVLGDSLT
                     LGREVFTHTMEEWIVRTLQYFVGKPNTQLVIRVHPGEMLTHGQSMVDVVQHVIPDLPE
                     HIHLIKPEDTTNTYDLIAAADLGLVYTTTVGLEMAMSGVPVVVVGNTHYADKGFTIDP
                     QSWVKYYKIIGSVLQDPVGHRLTDQQIDLAWAYAYRFFFDFPRPMPWHLVRMWEDYEQ
                     NPLSKVLSKEGRKEFEMTFRYLAGEPLDWVAIRDGKERFSG"
     gene            complement(1909..3186)
                     /locus_tag="PLL88_09235"
     CDS             complement(1909..3186)
                     /locus_tag="PLL88_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01787.1"
                     /translation="MSKIAIRVEDLGKKYNIGLPVVKYQTLRDKISESFKRREQVVDD
                     YIWALKEVSFEVKEGQVLGIVGRNGAGKSTLLKILSRVTEPTEGLVEIRGRVGSLLEV
                     GTGFHPELTGRENIFLNGAILGMRREEIQSKFDEIVDFAEVEKFIDTPVKRYSSGMYL
                     RLAFAVAAHLEPEILVVDEVLAVGDAEFQRKCLGKMSDVAQQGRTVLFVSHNMSAVLR
                     LTQETLVIDHGQLLLRAPTPEAVDFYLSTGLTQEGQKIWNEDEITTAAKPFQPIAIRV
                     LDKQGVVSDTVRSVEPFVIEVEYELEKSVKGLRVGIYLMTTRGEYIFTSFDTDDISQY
                     ESHTVRPAGRYISRCEIPANLLNEGRYVIGMNASTYLIKRYFQDDQALLFNVDPAGAP
                     GMQWVETRLGTVRPALKWNIEKNESGKLHHDKK"
     gene            complement(3208..4035)
                     /locus_tag="PLL88_09240"
     CDS             complement(3208..4035)
                     /locus_tag="PLL88_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01788.1"
                     /translation="MNEPRTIVIKPSTGWAALNLKDLWNYRELIYFFTWRDLKVRYKQ
                     TLLGASWAILQPVLTMVVFSIFFGGLAKVPSDGVPYPIFSYTALLPWTLFSKALQDAS
                     RSLVSSANIISKVYFPRLILPLSSTLAGLVDFLIAFAVLLLMMVFYGIKPTGNLWLLP
                     LLVLLALITAIGVSLWLSALNVMYRDVGHMLPFLNQFWMYITPIAYSSSLVPEEWRFI
                     YALNPLTGVVEGFRWALLGTGEGPGITLLISSCIAFLITISGLFYFRRMERKFADVV"
     gene            complement(4051..5013)
                     /locus_tag="PLL88_09245"
     CDS             complement(4051..5013)
                     /locus_tag="PLL88_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOJ01789.1"
                     /translation="MVTNSADFFARRRFCVTGGDGFLGRVVVRKLKERGAQDIFVPNI
                     KEYDLTQLASILKMLDDAQPDVIIHLAAQVGGIGANREHPGEFFYNNLMMGVQLMHEA
                     WKRSIEKFVAIGTICAYPKFTPVPFKEEDLWNGYPEETNAPYGLAKKMLLVQSQSYRQ
                     QYGFNSIFLLPVNLYGPGDNFDPASSHVIPALIRKCVQAVDEESQEIEVWGDGSPTRE
                     FLYVEDAAEGILLATEKYNDSEPVNLGSGFEISIKELVHLIADLTGYQGTFRWDTSKP
                     NGQPRRCLDTNRAQKFFGFKAQVTFEEGLKNTIAWYQENKGRMK"
     gene            complement(5003..5491)
                     /locus_tag="PLL88_09250"
     CDS             complement(5003..5491)
                     /locus_tag="PLL88_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HOJ01790.1"
                     /translation="MNTLQNHESRDVDILDHIENDPETTQATIAELLGVAVGTVNWHL
                     KRLIEKGYVKVRRIERRKLRYIITPQGIAYRASLLVDYIQSSFDMYRLVRKRSLEAIQ
                     KTKAQGFTSIFLEGSDDVAEICRLTCLEQGMQVSNEKIYPIFRIEGLKIFVIMEDAEN
                     GN"
     gene            5699..7135
                     /locus_tag="PLL88_09255"
     CDS             5699..7135
                     /locus_tag="PLL88_09255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ01791.1"
                     /translation="MHASEKFQQYQAIHREHWDGIAEQLDHWNGWGGYYRKRLSKIYQ
                     FHIPPAMDVLEIGCARGELLASVKPSRGVGVDLSFKMIERAKQRHPNLEFIQGDIHEL
                     ELNEKFDYIILSDLINDLWDVEAVLHKIQNWSKPSTRIILNYYNRLWEAPLALAQKLG
                     LAQRTLPQNWLSVDDVKNLLYLGGFEVIREWEEVLAPLPIPLLSSFCNGFLVRLWPFR
                     SLAMTAFTLARPIPNSSRHSKKAKVSVVVPARNEEGNIPQIFHRVPQMGSGTELIFVE
                     GHSKDNTYQAVEREMQKRPDVDAKLFRQEGKGKGDAVRLGFAHASGDVLMILDADLTV
                     PPEDLPRFYHAIESGRGDFINGVRLVYPMEKQAMRLFNYIGNKCFSLLFSFLLDQSVK
                     DTLCGTKVLWKKDYERIVANRSYFGDFDPFGDFDLLFGAAKMDLKIVDLPIRYAERMY
                     GTTNIQRWKHGWLLLKMVFFAARRIKFI"
     gene            complement(7132..7893)
                     /locus_tag="PLL88_09260"
     CDS             complement(7132..7893)
                     /locus_tag="PLL88_09260"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ01792.1"
                     /translation="MAYPQDVGADVDDPRVTYQRRTIIREKRLLHAIYEEWYSMQIAC
                     LPTSSGITLEIGSGAGFFKERNDRTLTSEVFYCPFVDAILDGTRIPLSAAALRSIVMT
                     DVFHHISDVEQFFREALRTLRKGGRIIMIEPWVSRWSRWVMDHFHSEPMDVSMQTWQF
                     PSHGPLSSSNQALPWIVFERDRILFEQKFPDLKVITLQPFMPFRYLLSGGVSMRALIP
                     AGCNAILKGIERKLDQRKWAMFALIVLEKKADRKP"
     gene            complement(7954..>8310)
                     /locus_tag="PLL88_09265"
     CDS             complement(7954..>8310)
                     /locus_tag="PLL88_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01793.1"
                     /translation="FITEMDVRMMDKYWNKLDSAATVLDPNIPRAITLFARSGNSSVD
                     FYEPTKEWEQLIYDPDPVAMAHAGYDYLYYDREYWKTLTPQEQTLLTSSCVVAVNEES
                     EWTGDYRVLLDISGCQ"
BASE COUNT         2204 a         2137 c         1895 g         2074 t
ORIGIN      
        1 cgtgcgagag gtcaagacaa acacgataag cgtacccttc ggaataagtc atatattccg
       61 gatactgaat aggtccggaa ccggcatcca ataaatattt tccatggtgg gaaagatggc
      121 gattaactcg caatggcatt gatgaatgta ctcgcgcgaa accgggcgca gatcctcata
      181 gcgagcattc tgataaacgc catcttccgt catctgccag ccgatactat cgtaaaagga
      241 ctttacttct tggatagtaa aatctttctc tctcatccag aaaatcgttc ctttccatcc
      301 cgaatagcaa cccaatccag cggttcacca gccagatagc gaaaggtcat ttcgaactct
      361 ttgcgccctt ctttcgacaa cacttttgac aaaggattct gttcgtaatc ttcccacatg
      421 cgcaccagat gccagggcat agggcgaggg aaatcaaaga aaaagcggta ggcatacgcc
      481 caggctaaat caatctgctg gtcagtcaag cgatgaccaa caggatcctg caaaaccgaa
      541 ccaatgatct tgtaatattt cacccacgat tgcgggtcga tggtaaaacc tttatcagcg
      601 taatgcgtat ttccaacaac gaccaccgga acaccgctca ttgccatttc aagcccaacc
      661 gtggtggtat acaccagacc cagatcagcc gcggcgatca ggtcgtaggt attggttgta
      721 tcttccggtt ttatcaaatg gatatgttcc ggcagatccg ggatgacatg ctggaccaca
      781 tcgaccatgg attgtccatg ggtcagcatt tcaccaggat ggacacgaat cacaagctgc
      841 gtattgggtt tacccacaaa atattgcaga gtccgcacga tccactcttc catggtgtgg
      901 gtgaacactt ctctccccag ggttaagcta tcacccaaca cattggtcgc cagcaagacg
      961 accgggcgtt catcatccaa acccaattcc ttccgggcgg ctgtcacgcc cttggctggt
     1021 gcacgctgcc agaggcgcga aaaatttttc caaatagacg ctttacgccg ggcttcaaac
     1081 aattcacgcg tcttgtgcag ttcactgcgt tccaggggtt tatcacggta agcttcccaa
     1141 agggcgtcag tttcttgctc catcaccttg gcatcttgag cgatccaaac ccgctgccgc
     1201 tggtcgccga attcatacgt ggttaccgga atattaagat acttggcaac ttcatatacc
     1261 acggcaaact cctggatagt gccgttaggg atgatcacca catctggttt gttggtggtt
     1321 aaccaagcca gggcgtttct ggctatatcc tcattccgct ccaggcgcat cttataaacc
     1381 ggttcatccg tatctactgt ttcaacctgc agagtatatt gcgaatcgaa tcgcgctacc
     1441 tgttcggctt tttgcgctaa ctcggcaggg agcgttttat atccggcata tacattaaga
     1501 aacgagaacg tttttacaaa gggattcagt ttcttcagaa tgctttgggc atacaggttc
     1561 tgacggcgca aatcgaagcg atttatcgga ttctgccagt catggtacgg caaatatccc
     1621 agagaaacgt catagcccaa accggtcagg gcggcgctga tcaatgccgc atggctgatc
     1681 cagtagtgca aagttgcaaa aaccaaaatt ttttttaccg gctctgcggc agtctggttc
     1741 gcgcagagat attgaacgat ctcaggaatg tgcttatcaa ggtcagtgag ggagaagcga
     1801 ctctgaatgc ccttgctgcg attgcggata agccaataca gctcagcggt taatggaacc
     1861 tcgccaagga catttttgat gaaatccctc gtttttcgct gtttgttatc actttttgtc
     1921 atgatgcaat ttaccacttt catttttctc gatgttccat ttcaatgccg ggcgtaccgt
     1981 tcccagtcgc gtttcaaccc actgcatgcc gggagcacct gccggatcga cattgaaaag
     2041 cagagcctga tcgtcttgaa aatatcgttt gatcagatag gtgctggcat tcatcccgat
     2101 cacataacga ccctcattca ataaatttgc aggaatttca cagcggctga tatacctgcc
     2161 agcagggcgc actgtatgac tttcatattg actgatatcg tcggtatcaa acgaagtgaa
     2221 aatatactct ccgcgcgtgg tcattaaata gatccccacc cgcaatcctt tcaccgattt
     2281 ttcgagttcg tattcaactt cgatcacaaa aggttcgacc gagcgcacag tatcagacac
     2341 aacgccttgc ttatccaaaa ccctgatcgc gatcggttgg aaaggttttg cggctgtagt
     2401 aatctcatct tcgttccaga tcttctgccc ttcctgcgta agccctgttg acagataaaa
     2461 atctaccgct tcgggggtcg gggcacgcag caacaactga ccatgatcaa tcaccagcgt
     2521 ttcttgggtc aatcggagca cggctgacat attgtggctg acaaaaagaa ctgtgcgtcc
     2581 ctgctgggca acgtcgctca tctttcccag gcattttctt tggaattccg catcaccgac
     2641 cgcgagaact tcatccacca ccaatatctc aggttcaagg tgagcagcta ccgcaaatgc
     2701 cagccgcaga tacataccgc tggaataccg ttttacggga gtgtcgatga acttttctac
     2761 ttccgcaaag tctacaattt cgtcgaattt actctggatc tcctcgcgcc gcatgcccaa
     2821 aatagcgccg tttaagaaaa tattctcacg ccctgtcaat tcaggatgaa aaccggtgcc
     2881 aacctcgagc agcgagccaa cccgcccgcg aatttcaacc aacccttctg tgggttccgt
     2941 aacgcgcgag aggatcttca acaacgtgct ttttccagcc ccgttacgcc ccacgatccc
     3001 cagcacctgc ccttctttca cttcaaaaga aacttccttc aacgcccaga tgtagtcgtc
     3061 aacaacctgc tcccgacgtt tgaaactttc ggatatcttg tcacgcaggg tctggtattt
     3121 gaccaccggc aagccgatat tgtatttctt acccagatct tcaactcgta ttgcgatttt
     3181 gctcatgcga tcttctttct cattgaccta aaccacatct gcaaacttgc gctccatacg
     3241 gcggaaatag aacaagccgg aaatggttat caagaacgca atgcatgatg aaataagcag
     3301 ggtgatcccc ggaccttcgc cggtgccgag aagtgcccaa cggaagcctt ccacaacgcc
     3361 ggtcaatggg ttcaaggcat aaatgaaccg ccattcttca ggaacgagag atgacgaata
     3421 agcgatgggg gtgatataca tccagaattg gttgagaaat ggaagcatgt gccccacatc
     3481 acggtacatg acattgagag cagaaagcca taagctcaca ccaatagccg tgatcaaagc
     3541 cagcaagacc agcaagggta acagccataa atttccggtc ggtttgatcc cataaaacac
     3601 catcatcaga agaagcaccg caaaagcgat caaaaaatca accagtcctg ccagggtcga
     3661 agacaaaggg aggatcaaac gcggaaaata tactttggaa atgatgtttg cgcttgaaac
     3721 cagagaacgg ctggcatctt gcaaggcttt cgagaacaac gtccatggca gcaatgcggt
     3781 atacgaaaag atcggatagg gaacaccgtc tgaagggact ttggcaagcc caccgaagaa
     3841 gatgctgaaa accaccatgg taagaacggg ttgcagaatc gcccagcttg cgcctaaaag
     3901 ggtctgcttg tagcgcactt ttagatcacg ccaggtgaag aaataaatca gctcgcggta
     3961 attccacaga tctttaaggt ttaaagcagc ccaccctgta gatggcttga tcacaatcgt
     4021 acgtggttca ttcatcgccg acatgttctg ctatttcatc cttcctttat tttcctgata
     4081 ccatgcaatg gtatttttca gcccttcttc gaaggtcacc tgagccttga aaccgaagaa
     4141 cttttgcgcc cggttcgtat ccaaacagcg tcggggctgc ccattgggtt tggaggtatc
     4201 ccagcggaac gtgccctggt aaccggtaag atcagcgatc agatgcacaa gctctttaat
     4261 gctgatttca aatccagaac cgagattgac cggttcagag tcattgtatt tttccgttgc
     4321 cagcaagatg ccttcggctg catcctcaac atacaagaat tcgcgggtcg gcgaaccatc
     4381 tccccatact tcgatttcct gactctcctc atcaaccgct tgaacgcatt tgcggataag
     4441 agccggaatg acgtgcgaag aggcagggtc aaagttatct ccaggaccat acaggttcac
     4501 cggaagcagg aaaatgctgt tgaaaccata ttgctggcga tatgactgag attgcaccaa
     4561 caacatcttt tttgccaacc cgtagggcgc attggtttct tcaggataac cattccagag
     4621 gtcttcttct ttgaagggaa caggggtgaa ctttgggtat gcacaaatcg ttccgattgc
     4681 cacaaatttt tcgattgagc gcttccaggc ttcatgcatt aattgcacgc ccatcatcag
     4741 gttgttataa aagaactcac ccgggtgttc acggtttgcg ccaattcctc ccacctgcgc
     4801 cgccaggtga atgatcacat ctggttgtgc gtcatccagc atttttagaa tagaagcgag
     4861 ctgagtgaga tcatactctt tgatgtttgg cacgaagata tcctgcgctc cacgttcttt
     4921 caacttgcgc accaccaccc gaccaaggaa accgtctccg ccggttacac aaaatctcct
     4981 tcgagcaaaa aaatcagcac tgttagttac cattctcagc atcctccata atgacaaaga
     5041 tcttcaatcc ttcgatacga aagattgggt atattttttc atttgaaacc tgcatgccct
     5101 gttcaagaca ggtcaacctg caaatttcag ccacatcgtc acttccttcc agaaatatgc
     5161 tcgtgaaccc ctgcgctttc gtcttttgta ttgcctccaa agaacgtttc cgcaccagcc
     5221 ggtacatatc gaaagaggat tggatgtagt caaccagcaa actggcgcgg tatgcgattc
     5281 cttgcggagt aatgatatag cgaagcttac ggcgttcgat gcgccgcact ttgacatacc
     5341 ctttttcaat taatcgcttt agatgccagt ttaccgttcc gacagccact cccaataatt
     5401 cagcgatggt ggcttgtgtg gtttcgggat cattctcgat atggtccaaa atatccacgt
     5461 cgcggctttc atgattttgt aaggtattca tgactcaata ttcaattgtt gaattataaa
     5521 acagggaaat tgatccgtca aggaagcggg caagaatgtg aattattcgt cacagtgcca
     5581 tccagaaacc accgtacaaa atgtcagcat ggagaacaaa aagagaaaaa ggtatctttc
     5641 atcggaccaa aatgatatac ttcctatgaa aaaatctcta agcaggtaac gaattttcat
     5701 gcacgcttct gaaaaattcc agcaatatca agctattcac cgggagcact gggacggaat
     5761 tgccgaacaa cttgaccatt ggaacggatg gggagggtat taccgcaaac gactcagcaa
     5821 gatctaccag ttccatattc cgccagccat ggacgtgctc gagatcggtt gcgcccgggg
     5881 ggagctctta gcctctgtga aaccatccag aggtgttggc gttgatctat ctttcaaaat
     5941 gatcgaaagg gcaaaacagc gccaccccaa tctggaattt attcaggggg atatccatga
     6001 gttggagttg aacgagaagt ttgactatat catcctttca gacctgatca acgatctatg
     6061 ggatgtggaa gccgtgcttc acaagataca aaactggtcg aagccgtcta cccgcattat
     6121 tctcaattat tacaaccggt tatgggaagc acctctggcc cttgcccaaa aactgggcct
     6181 tgcccagcgc accttaccgc aaaactggct atcggtggat gatgtgaaga atttgctcta
     6241 tttgggcggt tttgaggtca tccgcgaatg ggaagaagtg ctggctccct taccgatccc
     6301 gctcctctcc tcgttctgca acggcttctt agtgcggctt tggcctttcc gttcactagc
     6361 catgaccgcg ttcacgcttg ccagaccgat ccccaattcc agtcggcata gcaaaaaagc
     6421 aaaagtgagc gtggtagtgc ccgctcgcaa tgaagaaggc aatatcccgc agattttcca
     6481 tcgcgtacct caaatgggca gcggaacaga attgatcttc gtcgaaggtc attccaaaga
     6541 caacacctac caggcggtcg aaagagagat gcaaaagcgc cctgatgttg acgccaaact
     6601 attcaggcag gagggcaagg gaaaaggaga tgcggtcaga ttgggtttcg cgcacgccag
     6661 tggtgatgtt ttgatgatat tggatgcgga tcttacggta cctcccgaag atttaccccg
     6721 tttctatcac gccattgaat caggcagagg agattttatc aatggggtac gcctggttta
     6781 tccaatggaa aaacaggcta tgcgcctctt taattacatc ggcaacaagt gtttcagcct
     6841 cctcttctca tttcttttag accaatccgt caaggatacg ctctgcggca caaaagtatt
     6901 atggaagaag gactacgaga gaatcgtggc gaatcgctct tatttcggag atttcgaccc
     6961 gttcggcgat tttgatctgc tgtttggggc agcgaagatg gacctgaaga tcgttgatct
     7021 ccccattcgc tacgccgaac gcatgtacgg caccaccaac attcagcgct ggaaacacgg
     7081 ttggttgttg ttgaaaatgg tctttttcgc tgctcgccgc atcaagttta tttaaggttt
     7141 tcgatctgcc ttcttttcca aaacgatcag tgcaaacatc gcccatttgc gttgatccag
     7201 ctttcgttca attcccttta agatcgcatt acagccagcg gggatcaacg cgcgcatcga
     7261 aacccccccg cttaataagt agcggaaggg cataaacggt tgtagcgtaa tcactttcag
     7321 atcaggaaat ttttgctcga aaagaatcct gtctcgttca aaaacgatcc aaggcaatgc
     7381 ctgattagaa ctggaaagcg gaccgtgcga gggaaactgc caggtttgca tacttacatc
     7441 catcggctcg gagtggaaat gatccatcac ccaacgcgac cagcggctga cccagggttc
     7501 gatcatgatg atgcgtccac ctttgcgcag ggtgcgcaaa gcctcgcgaa agaattgctc
     7561 aacatccgaa atatgatgaa aaacatcggt catcacaatc gatcgcagag cagcagcaga
     7621 taagggaatg cgggttccat ccaaaatggc atctacaaac gggcagtaaa agacctcgga
     7681 ggtcaaggtt cgatcattgc gttctttaaa aaaaccagct ccggacccga tttccagggt
     7741 gatccctgag gaggttggca agcaggcgat ttgcatggaa taccattcct cataaatagc
     7801 atgcaacaac cgcttctcgc gaatgatcgt cctgcgctgg taggtaacgc gcggatcgtc
     7861 cacatccgct cccacatcct gtggatatgc caaaattctc ctgagcacgc tggggttttg
     7921 ttttgttctc ataacactgc ccgatggttg accttactgg caacctgaaa tatcgagcaa
     7981 gacacgatag tcgccagtcc actcgctctc ttcgttgacc gcaacaacgc aagaagaagt
     8041 gagcaacgtt tgctcttgtg gggtcagcgt tttccaatat tcccggtcat aatacaggta
     8101 gtcataaccg gcatgcgcca tagcaaccgg atcaggatca tagatcagtt gctcccactc
     8161 tttggtaggc tcatagaagt cgactgagga atttccactg cgcgcgaaga gggtgatagc
     8221 ccgcggtatg tttggatcta aaacagtcgc agcactgtct aatttattcc aatatttatc
     8281 catcatgcgc acatccattt cggtaataaa
//
LOCUS       DAOSDS010000090         8196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3255_length_8196_cov_4.729640, whole genome shotgun sequence.
ACCESSION   DAOSDS010000090 DAOSDS010000000
VERSION     DAOSDS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8196
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3255_length_8196_cov_4.729640"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="PLL88_09270"
     CDS             <1..260
                     /locus_tag="PLL88_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01794.1"
                     /translation="LLGLLALMVSNANVLQSISFFVTFAVGFGGNYALSALQKQAMSN
                     LSELISWPVVNVYLAIALMLWVLILLLLRRLDKDHVVRYMA"
     gene            complement(527..904)
                     /locus_tag="PLL88_09275"
     CDS             complement(527..904)
                     /locus_tag="PLL88_09275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ01795.1"
                     /translation="MRSTSSKTKWGFYVPKGQLKNYETPERAALREIKEELTLNRIPK
                     LVGKIGISDYSFHLPGDERPHYKIVHLFVYKLTNKAKIHPEKKENFIDARWFTFDEAI
                     NIIAFDREYLIRAKNLYVQRRNG"
     gene            1495..2430
                     /gene="cysK"
                     /locus_tag="PLL88_09280"
     CDS             1495..2430
                     /gene="cysK"
                     /locus_tag="PLL88_09280"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOJ01796.1"
                     /translation="MKIADDVTKLVGNTPLVRLNRITEGTVATVVAKLEFYSPAHSVK
                     DRIGVAMIDAAEKAGKIKPDTVIVEPTSGNTGIALAMVAAARGYKLILTMPETMSKER
                     RILLRAYGAELILTPGSEGMPGAVKRAEELTASDPDRYFLPQQFKNPANPEIHRETTA
                     EEIWKDTDGKVDFLVAGVGTGGTITGVGEVIKARKPSFKAIAVEPDASPVLSGGQKGP
                     HPIQGIGAGFIPDVLNTKIYDEVIRVTADAAFDTARRAAKEEGLLVGISSGAAIWAAL
                     QVARRPENEGKLIVVIIPSFGERYLSTALFAELAD"
     gene            complement(2492..3655)
                     /gene="moeB"
                     /locus_tag="PLL88_09285"
     CDS             complement(2492..3655)
                     /gene="moeB"
                     /locus_tag="PLL88_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HOJ01797.1"
                     /translation="MSMQQVFDLSPEEIQRYARHLVIPEIGLQGQLKLKKASVLIVGC
                     GGLGSPIALYLAALGIGRIGIVDYDTIELSNLQRQIIHSTNLIGTSKVQSARDRLLAI
                     NPEIQVDGYEELFTSNNAEKIAEPYQILIDGTDNIPTRYLMNDLCVLSGKPYVYGAVY
                     RFDGQLGVLDAKKGACYRCIFPVPPSPESVPNCSVASVVGVVPGIIGLLQAAEVVKLI
                     LDIGSPLYSQMVLYDSLECKMQTIQLPKNPACKVCGSNPEITRLIDYEQFCRMPIRDQ
                     YLNTEDPQGITPLELKEEMEKHAPIRLIDLRDPVELQISHIPGAENIPFHSLSERMKA
                     WDRQQEIILICHIGFLSSIAKQILIEAGFKNVRNLKGGMRAWASEPDSHANLY"
     gene            complement(3633..4781)
                     /gene="mnmA"
                     /locus_tag="PLL88_09290"
     CDS             complement(3633..4781)
                     /gene="mnmA"
                     /locus_tag="PLL88_09290"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOJ01798.1"
                     /translation="MNNKKETVLVGMSGGVDSTVAAYLLQKRGFHVIGITMQIWAGDP
                     SPSRPLRSGCYGPDEVYDIHDAEQTCKKLGIPHFIVDLSSEYEKTIIRDFFTTYTHGR
                     TPNPCVLCNPVIKFGALLERSSSLGIQFDHFATGHYARIIFHEASARYRLLKSMDSKK
                     DQSYFLYRLDQNQLMNTLFPLGDYRKDQVKAIAAENGFKTIAEKPESQDFTDGGGYKS
                     IFEKAQGKPGKIIDLQGQIVGEHPGIHHFTIGQRKGVLNGGHSQPLYILKIDPLTNCI
                     TVGPKEHLAVDLLTAMHTNWIAFEKLQEPINGLARLRSNQKESACTITPTDEDHIEVR
                     FEYPQSYATPGQSIVFYQDDLVLGGGIIQSTHHYVDNGKQSDEHATGV"
     gene            4985..6343
                     /locus_tag="PLL88_09295"
     CDS             4985..6343
                     /locus_tag="PLL88_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ01799.1"
                     /translation="MNKKFLLISLLSFFLLLFSASAVYAQADEAVAVNVYFFWGDGCP
                     HCAEEEPFLQSLEEKYPSITIVDYEVWYNEENQKILAEFGRVMNFEPTGVPVTIIADK
                     VWIGFREEYKADMENAILSELQNPSGFDAGLQISSTPEQSAGNQTSSKTITLPIFGKV
                     DLEKQSLIISTAIIGFVDGFNPCSLWVLSVLLALTIQSGSRKKIMIVGLTYLLVTAIV
                     YGLFITGVFTLLSYIGYLKWIQIIVAVIAIVFGLINLKDYFWYKKGLSFTISDKHKPN
                     LYKNMRSTVVNPRTIMGLIGSSAALAVGVSFIEFSCTAGFPVIWSNLIATNQVSVVSY
                     LLLLGLYMLIYLLDELAVFGVAVMTMQASRVEEKHGRVLKLISGTIMLALGVVMAIKP
                     AWMNELGTSLVVFLVAIAITVLVYLIHQKILPKFGIYIGNDSAHQKKKKKYRRRRSQD
                     EE"
     gene            complement(6525..7148)
                     /locus_tag="PLL88_09300"
     CDS             complement(6525..7148)
                     /locus_tag="PLL88_09300"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOJ01800.1"
                     /translation="MFTEVLPISHPETMGVAMEELKKGSVIAVPTDTVYGIACLLENE
                     PAIHEIFRIKERDTSKAVPLLIGEYEQIICASDGISESGQKLAKRFWPGPLTLVIPKR
                     LELPDVLSPYPTVGIRMPDHAWLCQLIRRVGVLAATSANISGGANPRTAQDVLHQLDG
                     RIPLIIDGGPCEGGVPSTVVDCSQEEIAILRIGAISEEQIQHALLAA"
     gene            complement(7164..8015)
                     /gene="prmC"
                     /locus_tag="PLL88_09305"
     CDS             complement(7164..8015)
                     /gene="prmC"
                     /locus_tag="PLL88_09305"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOJ01801.1"
                     /translation="MKSNTCLSNWLNQAHADLVRFHPEEPLSSLYAIVEKNTAFSRSW
                     LLANPQFPLDDGMLTRLNRDLQDLKDGKPLAYILGSWEFYGFSLHVSPAVLIPRPETE
                     LLVQSALDVLSQRKSPAIIADVGTGSACISIAIAKNQSQASIIATDISYAALEVAQKN
                     LTRYALKSRVHLLQSDLLSSVQGRFDVICANLPYIPTAALQDLPDLRHEPNLALDGGE
                     DGLAHIKRLLKQSTHRLAPRGHLFFEMQFDQAEALQQLAQLHFPDAEVIIKHDLSVHD
                     RLLVVHT"
     gene            complement(8005..>8196)
                     /locus_tag="PLL88_09310"
     CDS             complement(8005..>8196)
                     /locus_tag="PLL88_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOJ01802.1"
                     /translation="SGERAEKIRTYNYPQSRVTDHRIDISSHNLPSVMEGNLDMFIDE
                     LTLRDETERLSLVEEQGEE"
BASE COUNT         2064 a         1811 c         2008 g         2313 t
ORIGIN      
        1 ccttgctcgg tttgcttgcc ctgatggtaa gtaatgccaa tgtgcttcag tcgatatctt
       61 ttttcgtgac ctttgcagtt ggctttggtg gtaattacgc gttaagtgcg ttgcagaaac
      121 aagcaatgag caacttaagt gaactcattt cttggccagt ggtcaatgtg tatttggcga
      181 tagcgctgat gctttgggtg ttaatcttgt tgctgctacg gcgactagac aaggatcatg
      241 tagttcgtta catggcataa tggaacccca aagctagctt gtgtgtctgt tatatatctg
      301 caatgtgatt ttttggaatt attgggtgag gatgctctac taggacattt taactttgcc
      361 actacatcta gtattatctg aattgcatct tttccccaac taataatata tctatatccc
      421 tccgaatttg tttgatattc acttcttggt cacttcccgc attaattatt tcgtaaaagt
      481 tcgctcctag taattctata aataaatcaa ctattttcag actcttctat ccatttcgtc
      541 tctgtacata gagattcttt gcccttatca agtattcgcg atcaaacgcg ataatattaa
      601 tagcctcatc aaacgtaaac caccttgcat cgatgaagtt ttcttttttt tcaggatgga
      661 tctttgcttt atttgtcaat ttatatacaa acagatgaac aattttgtag tgtggtcttt
      721 catcgcctgg caaatgaaac gaataatcac tgatcccgat ttttcctaca agtttcggga
      781 ttctatttag tgtaagctcc tcttttattt ctctaagtgc tgcacgttca ggtgtttcat
      841 aattctttag ttgacccttt ggcacataaa agccccattt ggtttttgaa ctagtgctac
      901 gtataatgtc ccatctttgg gagattcata aaaaaataaa acctcctgct gattcatgcc
      961 taacaatttc atccttatgc ctaattttat tcataataag ccaccctttc tattggagat
     1021 cttgttttat cttcacaata gttataagac gtcttctcgt ataatagggt ttactatagg
     1081 gttgattata acaattctat tggatagatg gtcgggcaca gactatttct gcaaacatgg
     1141 cgttctggct ccatccttga gctccattgc tgatgccggt tgaagttctt cctttctacc
     1201 gcatctcctt tttgtatctt gacaatggaa atattgttgt gtataaattc attccaatag
     1261 aaacagactg atgatgaccc actttatttc actgattttc ggaatgtgca tgtgtatgac
     1321 cttacaacag ggagcaggaa aaacctggtg gtcatgttgt ggcttatccc gatccatttg
     1381 aaatgtcaac catcagtgaa tgctccccag cgaatgttgg tggagcggat tctacaaaaa
     1441 accgagaaac tgctctcggt ttttttttat ttcaaaaccg aaaggagaaa ggtaatgaaa
     1501 atcgcagatg atgtaacaaa gttggtgggg aatacccccc tggtacggct caaccgtatt
     1561 acggagggaa ctgttgccac agtggttgca aaacttgaat tttacagccc ggctcacagt
     1621 gtgaaagacc ggattggtgt ggcaatgatc gatgcggcag aaaaagctgg caagattaaa
     1681 cccgataccg taatcgttga accaaccagc ggaaatacgg gtattgctct tgcaatggta
     1741 gcagcggctc gaggatataa gctcatcttg accatgcctg aaacgatgag taaagaacgc
     1801 agaattctgt taagggcata tggagcagaa ttgatcctga cacccggcag tgaaggcatg
     1861 cctggcgcag ttaaacgggc ggaagaattg acagccagtg acccagaccg ttattttctc
     1921 cctcaacaat tcaaaaaccc tgctaacccg gaaattcatc gagaaaccac cgctgaagag
     1981 atctggaaag atacagatgg aaaggtggat tttttggttg cgggcgttgg cactggcgga
     2041 accatcacgg gggtgggcga agtgatcaaa gcgcgcaaac cgtcgttcaa agcgattgct
     2101 gttgaaccag atgcttctcc cgtcctttca ggagggcaaa aagggcctca tccgattcaa
     2161 ggaattggtg ccgggttcat tcctgatgtg ttgaatacga agatttacga tgaggtcatt
     2221 cgcgtaacgg ctgatgcggc tttcgataca gcccgccggg ctgccaaaga ggagggattg
     2281 ttggttggga tttcttctgg tgcagccatc tgggcagcgc tgcaggttgc cagaagaccg
     2341 gaaaacgaag gaaaattaat tgttgtaatc atcccttcgt ttggagaaag atatttaagc
     2401 actgccttat tcgcggaatt ggctgattag ttgattgtat ggttgaagaa atgaaaagac
     2461 cctctgtgca aatcgcacag agggtcactt tttaatagag gttagcatgt gaatcaggtt
     2521 cgcttgccca ggcgcgcatc ccgcctttta gatttctgac atttttgaaa cccgcctcga
     2581 tgagaatttg ctttgcaatc gaagataaaa atccaatatg acagattaaa atgatctctt
     2641 gctgcctgtc ccatgctttc attctttcgg aaagcgaatg aaaggggata ttttcggctc
     2701 ctgggatgtg agaaatttga agttcaaccg gatctcgcag gtcgatcagg cggataggtg
     2761 catgcttttc catttcctct ttcaattcaa gcggtgttat tccttgggga tcttcggtgt
     2821 tcaaatattg atcgcggata ggcatgcggc agaattgctc gtaatcgatc agtcgggtga
     2881 tctccggatt ggagccgcaa actttgcagg caggattttt aggaagttga atggtctgca
     2941 tcttgcattc caggctgtca tatagaacca tctggctgta caggggagat ccaatatcca
     3001 ggatgagctt tactacttcg gcagcctgta aaagaccgat gatgccgggg acaactccaa
     3061 ccacactggc tacggaacaa ttcggcacgg attctggaga gggggggacg ggaaagatgc
     3121 agcgataaca tgcccctttc tttgcgtcca gcactcccag ttgcccatca aagcggtaca
     3181 cggcaccata cacatagggt tttccgctca gtacgcaaag atcgttcatc aggtagcgag
     3241 tgggaatatt gtcggttcca tcaatcaaaa tctgatatgg ttcggcgatc ttttctgcgt
     3301 tgttcgaagt gaacagctct tcatagccat ccacttgaat ttcagggttg attgccagca
     3361 atcggtcgcg tgctgactgt actttggatg tgccgatgag gttggtgctg tggattatct
     3421 ggcgctgtaa attagacagt tcgatcgtat cgtaatccac gatcccaatg cgaccgattc
     3481 ccaaggctgc cagatacaga gcgatgggag atcccaatcc accgcatccg acgatcaaaa
     3541 ccgatgcctt tttcagtttt aactgtcctt gcaatccgat ctctggaatt acaagatggc
     3601 gcgcatatcg ttggatttct tctgggctga gatcaaacac ctgttgcatg ctcatctgat
     3661 tgtttcccgt tatccacata gtggtgtgta gactgtatga tgccaccgcc caggaccaag
     3721 tcgtcctgat agaagacaat ggactgtccg ggcgtggcgt acgattgtgg atattcaaat
     3781 cttacttcaa tgtggtcttc atctgtaggg gtgatggtac aggctgattc tttctgattg
     3841 gaacgcaggc gagcaagtcc attgatcggt tcctgtaatt tttcaaacgc gatccagttt
     3901 gtatgcattg ccgtaagaag atcgactgcc agatgttcct ttggtcccac cgtaatgcag
     3961 ttggtaagtg gatctatctt taagatatac aaaggctggc tgtgcccgcc attcaagaca
     4021 cctttccgtt gtccgattgt gaaatggtga atgcctgggt gttcaccaac gatttgaccc
     4081 tgtaaatcga tgatcttgcc tggctttccc tgtgcttttt caaatatgct cttgtatccg
     4141 ccgccatccg tgaaatcctg gctttctggc ttttcagcga tggttttgaa cccgttttcg
     4201 gctgcaattg cttttacctg atcctttctg taatcaccta gtgggaagag cgtgttcatt
     4261 aattgatttt gatccaacct gtacaagaaa tacgattgat ccttctttga gtccatgctt
     4321 ttcaacaaac ggtaacgggc gcttgcttca tggaaaatga tccgtgcgta atgaccggtg
     4381 gcaaaatgat caaattgtat ccctaaggaa ctcgaccttt ccagcaatgc gccgaatttg
     4441 atgaccgggt tacacaaaac gcatgggttg ggggtgcgac catgcgtata ggtagtgaaa
     4501 aagtctctga tgattgtctt ttcatattcg ctggaaagat ctacgataaa gtgtggaatg
     4561 cctaatttct tgcaggtttg ttcggcatcg tgtatatcat agacctcatc tggaccataa
     4621 caaccactcc gcagtgggcg gcttggggaa ggatctcctg cccaaatttg catggtaata
     4681 ccgatgacat ggaaccccct tttttgtaac aggtaagccg ccactgtgga atctacgcct
     4741 ccactcatgc ctaccaatac agtttctttt ttattattca tcaaccacaa tcctgcaata
     4801 gcaatttgag taaatatact ataactgtgg ctggaaaaaa taggaaactt ttcattcaga
     4861 agatctgaca gtttgttaat gatgagggta atttttatgt gaaaaaatca aaagtattca
     4921 tgttagaata tggcgtcaat ttgtatacaa gaaattgcga ttacagattt tgggcagagc
     4981 tgcaatgaat aaaaaatttc ttcttatttc cctgttatct ttttttctct tgcttttttc
     5041 ggcatccgcg gtttacgccc aggcggatga agccgtagcg gtgaacgttt attttttctg
     5101 gggcgatggc tgtccgcatt gtgcagaaga agagcctttt ttacaatccc tggaagaaaa
     5161 atatccgtcc atcacgatcg tggattatga agtgtggtac aacgaagaga accagaagat
     5221 ccttgccgaa ttcggcaggg tgatgaattt tgagccaacc ggagtgccgg tgacgatcat
     5281 tgcggataag gtttggattg gcttccggga agaatataaa gctgacatgg aaaatgccat
     5341 cctgagcgaa ttgcagaatc ccagtggctt cgatgccgga ttgcagattt cttcaacgcc
     5401 ggagcagagt gccggaaacc agacctccag taaaacgatt acccttccca ttttcggtaa
     5461 agtggacctg gaaaaacagt cattgatcat cagcaccgct atcattggct ttgtggacgg
     5521 tttcaaccct tgttctttgt gggtgctgag cgtgcttctg gcattgacaa tccaaagcgg
     5581 atcgcggaag aaaatcatga tcgtagggtt gacctacctt ctggtgacag ccattgtata
     5641 tggattgttc atcaccggtg tgttcacact gctgtcgtat atcggctacc tgaaatggat
     5701 ccagatcatt gttgcagtta tagccatcgt tttcgggttg atcaacctca aagactactt
     5761 ctggtataaa aaggggctgt catttacgat ctcagacaag cacaaaccca acctttataa
     5821 gaatatgcgc agcactgtcg tgaatccgcg cacgatcatg ggattgatcg gatcgtcagc
     5881 tgcattggcg gtgggcgtct cgttcattga attttcatgc acggcggggt tcccggttat
     5941 ctggagcaac ctgattgcca ccaatcaggt gagtgtggta agttatctgt tgctgctcgg
     6001 tttgtatatg ctcatctatc tgctcgatga attggcagta tttggagtag cggtcatgac
     6061 aatgcaagcc agccgtgtgg aagaaaagca cggacgtgtg ttgaagttga tcagtggaac
     6121 cattatgctc gctttagggg tcgtgatggc gatcaaaccc gcctggatga acgaattggg
     6181 aacgtcactg gtcgtgtttc tggtagccat tgccattacg gtcttggtct atcttatcca
     6241 ccaaaagatc ttgccaaaat tcggaattta tatcgggaat gatagtgccc atcagaagaa
     6301 aaagaaaaag tacagacgcc gcagaagcca agatgaagag tgatctgcaa tgagagcagc
     6361 cagtctttga ttgcacggat cggtgaaaaa tattgacgaa actcttttgc caacccgctt
     6421 gatttgatca tagcgctgtg cttgaccagc caaaaaccag aataatcaga taattgaacc
     6481 tgattgcagg aaaggttgaa tatcgccttg tctctgatga tcgttcaggc tgccaggagt
     6541 gcgtgctgga tctgctcttc gcttatcgcc ccaattctca ggatcgcaat ctcttcctgt
     6601 gaacagtcga ccacagtgga agggacgccg ccttcgcaag gaccgccgtc aataatgagc
     6661 gggatgcgcc catccagttg gtgaaggaca tcctgcgctg tgcgcggatt ggcaccgccg
     6721 gaaatattgg cagatgtggc agccaatacc cccactctgc gaatgagctg acacagccag
     6781 gcgtggtcgg gcatgcgtat tcctacggtg gggtacggtg aaagcacgtc gggcagttcc
     6841 agccgtttgg gaatgaccag cgtgagcgga cctggccaga aacgtttggc gagcttttgc
     6901 ccgctttctg atatcccatc gctggcacaa ataatctgtt cgtactcccc gatcagcaga
     6961 gggacggctt tggaagtgtc gcgttctttg atgcggaata tttcgtggat cgcaggctcg
     7021 ttctcaagca gacaggcgat gccgtacacc gtatctgtag gtacggcgat gacgctgcct
     7081 ttcttcaatt cttccattgc gacccccatg gtttcgggat gagatatggg caacacttct
     7141 gtgaacatgc atgcactcct tgtctaggtg tgtacgacca gcaggcggtc atgaacggat
     7201 agatcgtgct taattatcac ctcggcatct ggaaaatgca actgcgcaag ttgttggagt
     7261 gcttcagcct gatcgaactg catttcaaaa aaaagatgcc cgcgcggtgc gagacgatgg
     7321 gtggactgct tgagcaatct tttgatatgc gccagaccgt cctcgccacc gtccaatgcc
     7381 agatttggtt catgccgcag atcaggcaga tcttgtaagg cagcggtggg gatataggga
     7441 agattggcgc atatcacatc gaaccttccc tgcacgctgc tcagaaggtc gctctgcagc
     7501 aaatgaacgc gcgatttgag agcatatcgg gtgaggttct tttgagccac ttccagcgca
     7561 gcataggaaa tatccgtggc gatgatggat gcttgcgatt gattcttcgc gatggcgatg
     7621 gaaatacatg cgctgccggt gccaacgtct gcgattatgg caggcgactt cctctgagaa
     7681 agaacgtcca atgccgattg gaccagcagt tcggtttcgg gacggggaat gagcacagcc
     7741 ggtgagacat gcaaagaaaa cccgtagaac tcccaactgc ccaaaatata tgccagcggt
     7801 tttccatctt tcagatcctg aagatcgcga ttcagacggg tgagcatacc atcatccaat
     7861 ggaaattggg ggttagccag cagccaggac cgcgaaaaag cggtgttctt ttctacaatc
     7921 gcatacagcg aagaaagcgg ttcttcgggg tggaatctga ccagatcggc gtgtgcctgg
     7981 ttgagccaat tgctcaggca ggtgttactc ttcaccctgt tcttctacca gcgacaggcg
     8041 ttcggtttca tcgcgcaagg tcagttcatc gataaacatg tcgaggttgc cttccatgac
     8101 cgatggcaga ttgtgcgaag aaatgtcgat gcgatggtcg gtaacccgcg attgcgggta
     8161 attataggtt ctgatctttt ccgcgcgttc cccact
//
LOCUS       DAOSDS010000091         8189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3261_length_8189_cov_4.047578, whole genome shotgun sequence.
ACCESSION   DAOSDS010000091 DAOSDS010000000
VERSION     DAOSDS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8189
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3261_length_8189_cov_4.047578"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..972
                     /locus_tag="PLL88_09315"
     CDS             109..972
                     /locus_tag="PLL88_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOJ01803.1"
                     /translation="MSLITMKEILSESVAKRYAVGAFDTFDHSMTEAILRAAEEKERP
                     VILMIVEPLFQAPHFEWFFKYLVQRCEGSTVPVALHLDHGFSFEGVMKAIRYGCTSVM
                     MDGSNLPYAENVALTKKVVDVAHACGISVEAEIGHVAGHEGNMLEGNIPDESAFTKVE
                     DARRFVEDTGVDALAIAFGTVHGVFKGEPRLDYARLKKIRETVSIPLVMHGGSGLSPE
                     AFRNAIENGISKINFFTGLSLGGANAIIRMVEERRKKLQFGELVSAGMDGVSAIVREH
                     IDIFGTQSLDR"
     gene            1085..2047
                     /locus_tag="PLL88_09320"
     CDS             1085..2047
                     /locus_tag="PLL88_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOJ01804.1"
                     /translation="MKKILAFVMILGMVFMAIGCASTPAAETEEAAAAVEDEPILFGY
                     TAMDLTNPFFATIGDTIKVAVEANGDSVVIADGASDVDKQNTAIEDMITQGIDVLILN
                     PVNSEGIQPALEACAEAGIPVINIDSAVADLSLVKTYISSNNYQAGQVIGEEMVRIFP
                     DGGKLALLEAPQAESVVQRVMGIEDAIAGSNVVVADRRSITSMDKILNDAEDLIQANP
                     DLTAFYGANDQVALIILGVVQSAGLQDQIKVFGIDGSPSGKQSIADGGLYATAAQSPV
                     TMAQKAIELAYKVLTGEAIEATYSLDTTLITPQNIGDFDLENWN"
     gene            2100..3590
                     /locus_tag="PLL88_09325"
     CDS             2100..3590
                     /locus_tag="PLL88_09325"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOJ01805.1"
                     /translation="MPKNTPLLELKDIHKRFPGVYALQGVSFDLYSGEVHALLGENGA
                     GKSTLVKILSGIYSKDQGEIVIDGKPVKITNANEALENGLSVIHQELVLVPHLSIAEN
                     LFLGREPMLRNGLVDFKSMNVQTQKFLAEFGLNLEPEAEIASLTIAQQQMVEVVKAIS
                     FNARIIAMDEPTSSLTDKEVNSLFSHIRDLAKRGIGIIYISHRMSELFEIADRVTVLR
                     DGKYIGTRNVNETTADELITMTVGRPISKYYNRTYNTNNEVILEVKDLCSRKVKNVNF
                     DLRKGEILGFSGLVGAGRSETMRAILGIDKITGGTVEFEGEKIQVKNTAQMIEKGFSM
                     VPEDRKEEGIFLFQTLKFNLTLKTIKEFIHFIFVKNAKEDEIADKYMHSLSIKAPDTQ
                     TVMNSLSGGNQQKVVISSWLATQPKILILDEPTRGIDVGTKSEIYEIMNELAKKGVSI
                     MMISSDLPEILNLSDRVVVMCEGTTTAILDHTEATQEKVLQCAVKI"
     gene            3614..4603
                     /locus_tag="PLL88_09330"
     CDS             3614..4603
                     /locus_tag="PLL88_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01806.1"
                     /translation="MTVNTLKNNVQKNGLLLSIKRNLGILVGLAILCTIIAINSPVFL
                     TTKNIMNVLRQISANLFVACGMTIILIAGGIDLSVGSTIAVVGVAAASFLDMGMSVPL
                     TVLACLGVGAFIGFINGFIISRTKITPFIVTLSMANVLRGLAYVYTGATTIRINDRGY
                     INIGTGFLGPVPLPAIYIAVVLMIVYLILNKSRLGRHIYAIGGNEKAARFSGIDVKKV
                     RLLIYIFSGLMAAVASITLSARSYSGNPTAGEGAEMDAIAAVVLGGISMAGGNGHISG
                     TIIGALIIGVINNGLNLMGIHSFWQMILKGVIILVAVYADYMKNARSNKASEA"
     gene            4625..5659
                     /locus_tag="PLL88_09335"
     CDS             4625..5659
                     /locus_tag="PLL88_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ01807.1"
                     /translation="MKKNFVNQLAERLGYPNSRPLLAILERIVSEDEAEWMAHLPATP
                     YDLALRMRMDETSAAAGLEDLFMRGLVLISDQTPAGPLYVFDANPGRFMDMILIDPRY
                     KVYGQEFYDLWRDFYNNELVYKARSAEKFSFRIVPVGQKIEDKRSILPQERAEMIVRN
                     AKKIVVQECPCRVRERECDNPLEVCLALDKTAQYNLSRGLGREISTDEASQVIAEAEK
                     AGLIHETDNTEHPTVLCNCCTCCCVFLKAIAVYQQAHVISKSRYYAHIEAQECSVCGV
                     CLERCHFGAIFRTDDGMLVDSQRCYGCGLCSSTCPSDAITLRMRDPQEIAPFNGGEFM
                     KGMTKVPAQK"
     gene            5713..6651
                     /locus_tag="PLL88_09340"
     CDS             5713..6651
                     /locus_tag="PLL88_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HOJ01808.1"
                     /translation="MSCDVACVGMALTDVLVKGVNLQNKYTYEARMAESVNLWVGGDA
                     ANESIVLAHLGVKTKLIAGVGADATGTFIRTVISSQGVNTDDFATVSDYSSLVSIVMI
                     DASGERNFIFAGAITGDHFKPNLEALRGAKIVSLGSMGVPPISNNESILRIVKTAKEN
                     GSIVCADAVYMPGGFTYADLKESLQYIDYVFPNLDEAKALTGKQEIDEVAKTILDYGV
                     KHAIVKQGKDGCWAFAGDEKLFVPAYKVNTVDTTGAGDNFMAGFMCAILDGKDLEGCC
                     KFATAAAAVSIQTIGANTGVKNKQQVLDFMKAMEAS"
     gene            6703..7491
                     /locus_tag="PLL88_09345"
     CDS             6703..7491
                     /locus_tag="PLL88_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HOJ01809.1"
                     /translation="MINIQCNFRAKSLYMPMTINVLLPSFFEHAATVESLDDVYKDRQ
                     FSYKTLYLLHGGVEDAHSWLAYTNIEKYADKHKLAVVLPSVGNSFYANLVRGPAYWTF
                     VSEELPRFVQSVFPLSDKREDNFVAGLSMGGYGAFKMALNHPDRYAAAISLSGALDIV
                     GVLTAPNMIQSKLEDYFENLDAVQGTYNDLFYQAKKIKEAGFHFPRLYMACGVDDFIY
                     EVNQKYLKFLSDEKIPVTYEEGPGAHEWNFWDKYVERAIDWLFQ"
     gene            complement(7751..>8189)
                     /locus_tag="PLL88_09350"
     CDS             complement(7751..>8189)
                     /locus_tag="PLL88_09350"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01810.1"
                     /translation="GVPVHTHTEIGTWGFEQIEIIESEGMDLTRFGLAHLDRNPDFWY
                     HKKIAEKGCYIIYDGPGKAKYYPDSIRVDLLRRLMDAGFEKQIMLCNDMGKKSHHTVY
                     GGGPGWTWIKQKFLPRLLDEGFSQETIDNFMIHNPARFYSLKE"
BASE COUNT         2168 a         1725 c         2094 g         2202 t
ORIGIN      
        1 cgattctgaa aatggaagcg taagctcatg acaaatttgc cctccgagaa gttagggagg
       61 ggttgactca acaccacgaa aaattcatta gaaaagaaga gagaaaaaat gtctttgatt
      121 accatgaaag aaattttgag tgaatcggtt gcgaaacgct atgcagtagg tgcctttgat
      181 acttttgacc attccatgac cgaagccatt ttgcgcgcag ccgaagaaaa agagcgaccg
      241 gtcattttga tgatagttga acccctgttt caggcacctc attttgagtg gttctttaaa
      301 tacttggttc aacgctgtga agggtcaacc gtgccggtag cgctgcatct tgatcatggg
      361 ttttcttttg agggtgtgat gaaagccatt cgttatggct gtacttctgt gatgatggat
      421 ggttccaacc ttccctatgc ggaaaatgtt gcgctgacca aaaaagtggt cgatgtcgct
      481 catgcttgtg gcattagcgt agaagcggaa atcggtcacg tggcagggca tgaaggcaat
      541 atgctggaag gtaatatacc tgatgaaagc gcctttacca aagtggaaga tgcacgtcgt
      601 tttgtggaag acaccggcgt ggatgcgttg gcgatcgctt ttgggactgt tcacggagtt
      661 tttaaaggag agccgagatt ggattatgcg cgcttgaaaa agattcgcga aaccgtttct
      721 attcctctgg tgatgcatgg cggatcaggg ttatctccag aggcttttcg aaatgcaatt
      781 gagaatggga taagcaaaat taatttcttc accggtttgt ccctgggtgg ggcgaacgcg
      841 attattagaa tggtggagga aaggaggaaa aaattgcagt tcggagaatt ggtcagtgcc
      901 ggtatggatg gtgtaagtgc catcgtgcgg gaacatattg atatcttcgg tacccaatcg
      961 cttgatcgat aaaacgctga ttttccatca cttatgcgtt aggtagatga cgccgaacag
     1021 caacaataac aaaagattaa ttcctgaaaa tcaaaacaat taacgattat aggagaatta
     1081 agagatgaag aaaattttag cttttgtgat gattcttggt atggtcttca tggcaatcgg
     1141 ttgcgccagc acccctgccg ctgaaacgga agaagccgca gctgccgttg aagatgaacc
     1201 catcttgttt ggttacactg caatggattt aaccaatccg ttcttcgcaa ccattggcga
     1261 cacgatcaaa gtagccgtag aagccaacgg tgatagtgtt gtgatcgctg atggtgcgtc
     1321 cgatgttgat aaacagaaca ctgccattga agacatgatc acccagggta tcgatgtttt
     1381 gattctgaat cccgtcaatt ccgaaggcat tcagccagcc ctcgaagcat gtgcagaggc
     1441 tggaatccct gtcatcaaca tcgactctgc agtggctgac ctgtcactgg taaagaccta
     1501 tatttcttcc aataactacc aggctggaca ggtcattggt gaggagatgg ttcgtatctt
     1561 ccccgatggc ggaaagcttg ccttgctgga agccccccaa gccgaaagtg ttgtgcaacg
     1621 tgtaatgggt attgaagacg ccatcgccgg cagcaacgtt gttgttgctg atcgtcgctc
     1681 catcacttcc atggataaaa tcttgaatga tgccgaagac ttgatccagg ctaatcctga
     1741 cctgaccgcc ttctatggtg ccaatgacca ggttgctctg attatcttgg gtgttgtcca
     1801 atccgccggt ttgcaggacc agatcaaagt attcggtatt gatggttctc cctccggcaa
     1861 acaaagcatc gccgatggtg gcttgtatgc gaccgctgct caaagcccgg tcactatggc
     1921 tcaaaaagca attgaattag cctataaagt tttgactggg gaagcgattg aagctaccta
     1981 ttcactcgat actactctga tcactccgca gaatatcggt gatttcgatc tcgaaaattg
     2041 gaattagata gatttgatct gattctggtt cgataaagaa agattgtatg ggagatggga
     2101 tgcctaagaa tacaccgctg cttgaattaa aagatattca taaaagattt ccgggcgtgt
     2161 atgctttgca gggagtcagt tttgatctct actctggcga agtgcatgcg ctgcttggag
     2221 aaaatggcgc tggaaaatct acgttggtca agatcttgag cggtatttac tccaaagacc
     2281 aaggtgagat cgtgatcgat ggaaaaccgg tgaagatcac caatgccaat gaggcgttgg
     2341 aaaatggatt gagtgtcatt catcaagaat tggtgctggt accccatctc tctattgcag
     2401 aaaatctctt tttgggtcgc gagccgatgc tcaggaatgg attggttgat ttcaaatcta
     2461 tgaatgtgca gacccagaaa tttttagcag aattcggttt aaatctagaa cctgaagctg
     2521 aaattgccag tttgactatt gcacagcagc aaatggttga ggtggtaaag gcgatctcct
     2581 tcaatgcgcg catcattgcc atggatgaac caacctcatc cttgacggat aaagaagtta
     2641 attctctatt cagccatatc cgtgacttag ccaaacgggg cattgggatt atctatatct
     2701 ctcaccgcat gagtgagttg tttgagattg cagatcgtgt gaccgtatta cgggacggaa
     2761 aatatattgg cacgcgcaat gtaaacgaaa ccactgcaga tgaattgatc actatgaccg
     2821 tggggcgtcc tatttcgaaa tattacaatc gaacgtacaa cacaaataac gaagtgatcc
     2881 tggaggtaaa ggacctgtgc agcaggaaag taaaaaatgt caattttgac ctgcgtaaag
     2941 gcgagatctt agggttttcc ggtctggtag gtgcaggtcg ttctgaaacc atgcgagcta
     3001 tcctggggat cgataagatt actggcggta ccgttgaatt cgaaggggaa aagattcagg
     3061 tcaaaaatac tgctcaaatg atcgagaagg gcttcagcat ggtcccagaa gatcgaaaag
     3121 aagaagggat tttcctgttt caaacgttga aattcaattt gacgctaaaa acgattaaag
     3181 aattcatcca tttcattttc gtaaaaaatg ccaaagaaga tgaaattgca gataaataca
     3241 tgcatagctt atccattaaa gcccccgaca cgcaaacggt gatgaattct ctttcgggtg
     3301 ggaatcaaca aaaagtggtc atctcaagct ggttggcaac ccaacccaag attttgattc
     3361 ttgatgaacc gacccgaggg attgacgtgg gtacaaaaag cgaaatttac gaaatcatga
     3421 atgaactggc taagaaaggc gtttccatca tgatgatctc ttccgatttg ccggaaatat
     3481 tgaacttaag tgatcgggtt gtggtgatgt gcgaaggaac caccactgcc atacttgatc
     3541 acacagaagc aacacaagaa aaggtccttc agtgcgcggt gaaaatctag gattggagaa
     3601 aaggattgga aatatgactg taaatacgct aaaaaataac gtccaaaaga atggtctcct
     3661 gttgagcatt aaacggaatc tcggtatttt ggtaggactg gctattctgt gcacgatcat
     3721 cgcgattaac tcaccagtgt tcctgaccac caagaacatc atgaatgtcc tgcggcagat
     3781 ctccgcgaat ttgttcgtcg cttgtggtat gactattatt ttgatcgcag gtggcattga
     3841 tctttctgtt ggatcaacca tcgctgtggt tggtgtggca gccgcctcat ttttagatat
     3901 gggcatgtcc gttcccttga ccgtgttggc ttgcttgggc gtaggcgctt tcattggatt
     3961 cattaatggt tttattattt cacgtaccaa gattacgcca ttcatcgtaa cgctttctat
     4021 ggcaaatgtg ttgcgcggtt tggcttatgt gtataccggc gctacaacca ttcgtattaa
     4081 tgaccgcgga tatatcaata ttggtaccgg tttccttgga ccggttcctc tccctgcgat
     4141 ctatatcgcc gttgtcttaa tgattgtcta cctcattttg aataagagcc gtttaggtcg
     4201 tcatatctat gccattggtg gcaatgaaaa agctgcccgt tttagtggta tcgatgtaaa
     4261 aaaagtgcgt ttgctcattt atattttcag tgggctcatg gcagcggtgg caagtattac
     4321 cctctccgcg cgcagttact cggggaatcc aactgccggc gaaggcgcgg agatggatgc
     4381 catcgcggcg gttgtgttgg ggggtatcag tatggctgga ggaaatggtc acatcagcgg
     4441 taccatcatc ggtgcgctga ttattggcgt cattaataat ggtcttaacc tgatgggtat
     4501 tcactccttc tggcagatga tcctgaaggg cgtcatcatc ctggtagcgg tgtatgcaga
     4561 ttacatgaag aatgccagga gcaataaagc cagtgaagca tagactggat aaataagaag
     4621 gaagatgaaa aagaatttcg tcaaccagct tgcggaacgc ttgggatacc ccaattcgag
     4681 acctctcctg gctattcttg aaaggatcgt ttctgaggat gaagcagaat ggatggcgca
     4741 tttacctgct actccgtatg atctggcgtt gagaatgaga atggatgaga ccagcgctgc
     4801 agcgggtttg gaagatcttt ttatgcgcgg tctggtcctt atctctgatc aaacccctgc
     4861 gggtccactg tatgtgttcg atgccaaccc gggacgtttc atggatatga tcctgatcga
     4921 tccgcgttat aaggtgtacg ggcaggaatt ctatgacctg tggcgtgatt tttataacaa
     4981 tgagctagtc tataaagccc gttctgcgga aaagttctct tttcgcatcg tgccggtggg
     5041 tcaaaagata gaggataaac gttccatcct gcctcaagag cgagcggaga tgatcgtaag
     5101 aaacgctaaa aagatcgtgg tgcaggaatg cccctgccgg gtgcgcgaac gggaatgtga
     5161 taacccactg gaggtctgcc tggcattgga taaaactgcg caatataacc tgagccgcgg
     5221 gcttggacgc gagatcagta cggatgaagc atcgcaagtc atagcggagg cggagaaagc
     5281 cggtcttatt catgagaccg ataatacaga acatccaaca gtcttgtgca actgctgtac
     5341 ctgttgctgt gtgtttctca aagccattgc ggtttatcaa caggcgcatg tgatttccaa
     5401 gagccgctat tatgcgcata tcgaagcaca ggaatgtagc gtttgcgggg tttgcctgga
     5461 gcgttgccat ttcggcgcta ttttccggac agatgatggt atgttggtcg attcacagag
     5521 atgctacggt tgtggattgt gctcatcaac ctgcccgtcg gatgctatca cattacgcat
     5581 gcgcgacccg caagagatag cccccttcaa tggaggtgag tttatgaagg gcatgacgaa
     5641 agtacctgct cagaagtaaa gtgtccaggt ctttctttga agtgatgata tttcaaaacc
     5701 gaggaaaatg ttatgagttg tgatgtggca tgtgtcggaa tggctttgac ggatgtgctc
     5761 gtcaaaggtg tgaacctgca gaacaaatat acctatgaag cacgcatggc ggaaagtgtt
     5821 aacctgtggg tgggtggaga tgccgccaac gagtccattg tgcttgccca tttgggagtt
     5881 aagaccaaat tgattgccgg tgtgggtgcg gacgctactg gtacttttat aagaaccgtt
     5941 atctcttccc agggtgtgaa tactgatgat tttgctaccg tctctgatta cagttctctg
     6001 gtcagcatcg ttatgatcga tgccagcggg gaacgcaatt tcattttcgc cggtgccata
     6061 acaggcgatc attttaagcc aaacctggaa gcgctcaggg gcgcaaagat cgtgtcattg
     6121 gggagtatgg gtgtgccgcc catttctaac aatgagagca ttttacgcat cgtcaaaacc
     6181 gcaaaagaaa atggttccat cgtctgtgcc gatgctgtat atatgccggg tgggtttacc
     6241 tacgctgatc tcaaagagtc gttgcagtat attgattatg tttttcctaa cctggatgaa
     6301 gccaaagcgc tgaccggcaa gcaagagatc gacgaggtcg ctaaaaccat cctcgattat
     6361 ggtgtcaagc atgccatcgt caagcaggga aaagatggct gctgggcatt tgccggggat
     6421 gagaagctat tcgtaccggc gtataaagtg aacacggtag ataccaccgg tgccggcgat
     6481 aactttatgg cgggtttcat gtgcgccata ttagatggaa aagacctgga aggttgttgc
     6541 aaatttgcca ctgccgccgc tgctgtttcc attcagacca tcggagccaa cacaggtgtg
     6601 aaaaacaaac aacaggtgct cgattttatg aaagccatgg aggcttctta gctcttatct
     6661 ttttagtgat agaaaaatta aagatgtgga aggaactcac tcatgatcaa cattcaatgc
     6721 aattttcgcg caaaatcact ctatatgcca atgaccatca atgtactgct gccgtctttc
     6781 tttgaacatg ctgccacggt ggaatcgttg gatgacgtgt ataaggatag gcaatttagc
     6841 tacaagacgc tttacctgtt gcatggtggc gtggaagatg cccattcctg gctggcatat
     6901 accaacatcg aaaagtatgc cgataaacac aaactggcgg ttgtactgcc cagtgtaggc
     6961 aacagtttct atgccaacct ggtgcgtgga ccggcttatt ggaccttcgt cagtgaggaa
     7021 ctgccgcgtt ttgtgcaatc ggtttttccg ctctcggata aacgcgaaga taattttgta
     7081 gccgggcttt ccatgggcgg ctatggcgca ttcaagatgg cgctcaacca tcccgaccgt
     7141 tatgcagcag ccatcagtct atcaggcgca ctggatatcg tgggtgtgct taccgcaccc
     7201 aatatgatcc agagcaagct ggaagattat ttcgagaacc tggatgccgt gcagggcacg
     7261 tataatgact tgttctacca ggcaaagaag atcaaagaag cagggtttca cttcccacgg
     7321 ttgtatatgg cctgtggcgt ggacgatttc atttatgagg tcaatcaaaa atacttgaaa
     7381 ttcctgtctg atgaaaagat ccctgtgacc tatgaagagg gtcccggggc gcacgaatgg
     7441 aatttctggg ataagtatgt tgagcgcgcc atcgactggc tatttcagta atcaaacttc
     7501 tctctttatc attattctca agggtctccc ctgtgtcatt aatggtaggc aggggagaat
     7561 tccttaaaaa cgctgttctg tgtttccact atctatccga agaggtaacc tggctaggaa
     7621 ttttctgata aagatgagtg catcggaacc agcacgttcc atttcaacag gaaagtgatc
     7681 gcctgcttcc ccatcaccga tcaaatccgt gatggagctt ttaacagaac cctccttatt
     7741 ttcgagaaac tcactccttc aacgaataga atctggcagg gttgtggatc atgaaattat
     7801 cgatggtctc ctggctgaat ccctcatcca gcaagcgcgg caggaatttt tgctttatcc
     7861 aggtccagcc tggtccgccg ccgtacaccg tatggtgcga tttcttaccc atgtcattgc
     7921 atagcatgat ctgtttttca aagccagcat ccattaaccg acgcagaaga tcgacgcgaa
     7981 tactatcggg gtaatatttg gcttttccgg gaccgtcgta gatgatgtag cagccttttt
     8041 cggcgatttt cttgtgatac cagaaatctg gattgcgatc gagatgtgca agaccaaagc
     8101 gggtgagatc catgccttcc gattcgataa tttcgatctg ttcgaatccc caggttccta
     8161 tctcggtgtg agtgtggacg ggcacaccc
//
LOCUS       DAOSDS010000092         8136 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3281_length_8136_cov_3.328672, whole genome shotgun sequence.
ACCESSION   DAOSDS010000092 DAOSDS010000000
VERSION     DAOSDS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3281_length_8136_cov_3.328672"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..968
                     /locus_tag="PLL88_09355"
     CDS             <1..968
                     /locus_tag="PLL88_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01811.1"
                     /translation="KGGPLTTKELMQQIEFNTVENQRLAEFSLNLALQEDERYDEVGP
                     SGEVLWYLRSMEPEDVKKTPLFLQYKEMPVENPQVNQYLALFEGTLADELEVDEPLNS
                     DVTVTISLLFPHWRAGTLPFSNALSHIFPTAFESPRVRFTFRDEDTDETFNGWVVRKQ
                     RYVFGLRDWYEKKGVIPGSFLSLKKGSNPGEIVIRGEKSRQNKEWIKTVLVGADKGIV
                     FAMLKHPIEISFNERMSIAIPNTEALDAIWKEREKLNPDKVFLQIAHELAKLNPQGHI
                     HAQEMYAAVNVVFRCPPSPVLNFLLSSSAIQHLGDLYFHIKEGEG"
     gene            975..1409
                     /locus_tag="PLL88_09360"
     CDS             975..1409
                     /locus_tag="PLL88_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01812.1"
                     /translation="MNPAGHFSLVKPTLETPFHIDFDWWIEHDANWRVFLLDFLCDEH
                     RALYVEKDQNIQIDAIDPQTAEVKRVDGLLSTLVDHCAQQEGFISKNTTLVNMIFKIF
                     LANDNAPLNANQLAELTGKPAMTILRTFSGVQVFKGIRPLQE"
     gene            1450..1523
                     /locus_tag="PLL88_09365"
     tRNA            1450..1523
                     /locus_tag="PLL88_09365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1484..1486,aa:Arg,seq:tct)
     gene            complement(1604..2545)
                     /locus_tag="PLL88_09370"
     CDS             complement(1604..2545)
                     /locus_tag="PLL88_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOJ01813.1"
                     /translation="MIKTEPITANYLRLQRLSTEDGPGTRTTIFLKGCTLQCAWCRSP
                     ESIARVATHIRWLEHFCIGCKTCVLSCPGGCISMEDRGLRIDYSRCVLCGKCAEACPA
                     NALELPWKKIEAQELSEELLRDKTSFARSGGGVTFSGGEPALQPEFCIALMKILKEQG
                     IHIVLNTCGAVPQENLEMILPYTDLLLYDLKLMNAEQHKQLTGADNERILANLLWIAD
                     ILRGREYHFTLWIRTPLIPHATADKENLQAIAAFLNQHMENLVERWELRTFNQLRRDQ
                     YQASMSRAKLAACQLWASQIYHQPQTVLITGSARAEE"
     gene            2764..3456
                     /locus_tag="PLL88_09375"
     CDS             2764..3456
                     /locus_tag="PLL88_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOJ01814.1"
                     /translation="MYGYGLYLLISLPALILGLWAQAKVQGAYKKYSQVRTSNGMNGA
                     DVARHILDSNGLSSVAIKQTGGTLSDNYDPRNRTLNLSTGVYQSNSIAAAGVAAHEAG
                     HAIQHKESYGPLKVRSLMIPTVQLGSWLGPILFFVGYFFSNYNLALIGVILFAGIALF
                     SILTIPVELDASKRAKAILASEGILYGGEMEGVSQVLDAAAWTYVAAATQAISTVLYY
                     VLLLGGRSSRRR"
     gene            complement(3525..4532)
                     /locus_tag="PLL88_09380"
     CDS             complement(3525..4532)
                     /locus_tag="PLL88_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ01815.1"
                     /translation="MASFSRGLGIDLGTTNTVIAENGQILLHEPTAVSVLLEEWKMIE
                     VGQAAKDMTGRVPDSIEVYFPIQNGVIAEYEITENFLKYQIHRIVGSMLLFRPSLMLT
                     VPYGITSVERRAVHEAGLGAGGREVLLIEAPLAAALGIDLPIAPPSGNMIICLGGGTN
                     QVAVLAMNSIISAEIERGGGNKLDLDIIDHVRKTYGVILSRFSAEIVKMRIGAAIEGI
                     EESEMEVQGQDQVTGLPRSVTLRTQDIIEAVQEGLQLVTKLVRRTLEKTPPELISDIM
                     DRGVALCGGGTQLRGVERYLTKELGIPAYLVDNPTTCTAEGAARALGMLDELKRSLPV
                     T"
     gene            complement(4584..5162)
                     /gene="ruvA"
                     /locus_tag="PLL88_09385"
     CDS             complement(4584..5162)
                     /gene="ruvA"
                     /locus_tag="PLL88_09385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOJ01816.1"
                     /translation="MIASLKGIVTFKNDHSLVVETGGIGFKISCTKATCAQAEVGNHI
                     SLYTHHITREDGMFLYGFEKIEERDLFIQILSVSGIGPKLGLNLLSSLSAEAIQRAVM
                     KEQDEVFSHVPGIGEKTAKKIVLMLKDKIAPLLEEAGLEEIPDTDTDVLQALVGLGYS
                     LYEAQKAVASIPRDAPQTPEERLRLALQYFSA"
     gene            complement(5159..5959)
                     /locus_tag="PLL88_09390"
     CDS             complement(5159..5959)
                     /locus_tag="PLL88_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOJ01817.1"
                     /translation="MITSTSNTQIKEIRSLNQRKFRKESSLFYVEGLRAIASALENRA
                     SVEKVVFCAELLESDFGKSLLQRAEKAGIPVLEVSRAVFESISLKENPQGMCAVVRQN
                     VSDLLLSTTVTGLWVALEGVQDPGNLGSILRTLDAVGGKGLILIGEGTDAFHPTSVRA
                     STGAIFSTQLYQCEVPTFIRWKQEQTIDLIGAVCDAHHDYRDYNYAENTILCMGSEQK
                     GLPASLQQVCTSLVTIPMIGTVDSLNLANAASVLLYEYFNQHHPLRKR"
     gene            complement(5961..6452)
                     /gene="ruvC"
                     /locus_tag="PLL88_09395"
     CDS             complement(5961..6452)
                     /gene="ruvC"
                     /locus_tag="PLL88_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOJ01818.1"
                     /translation="MLVIGLDPGTAITGYGIIDELPDRSLRLLAHGTIRTSSAMDTDQ
                     RLQVLHEKLTEILESYQPQCAAVEKLYFQRNVRTALQVGQARGVIMLALSQNSIPVYE
                     YNPMDVKLAVTGYGMAEKGQVQQMVKALLNLHEIPKPDDAADALAIAICHAHSARLTM
                     LDT"
     gene            complement(6456..7214)
                     /locus_tag="PLL88_09400"
     CDS             complement(6456..7214)
                     /locus_tag="PLL88_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ01819.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGQLFTRITREIVIAVRESGSDLE
                     GNFRLRIAIDRARAVNMPKDNIDRAIKRGTGEDKDGEVIEQITYEGYAPHGIALMVEC
                     LTENRNRTVSEIRHVLTRAGGNMGEVGSVSWQFDRIAYFGIEGEGVNFDKVFELALEG
                     GANDITQDEEGYIEIFAPVEAFKTINDLLKEAKIEVDEAELRMVPKQEIVLDNEQTVK
                     VMRLVEDLEDLDDVQSVYSNLEVSDEALASMEEE"
     gene            complement(7320..8099)
                     /locus_tag="PLL88_09405"
     CDS             complement(7320..8099)
                     /locus_tag="PLL88_09405"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOJ01820.1"
                     /translation="MRIVRYQIADQEPRFGWETEGRVGPLSASPLGLYRRLELEHDLE
                     KVRLLPPILPGKIIAVGRNYPLHAQEQSVEIPEIPIIFLKPLSAVIGPDQAIQLPPQS
                     QHVEHEVELAVVLGKGGRWIRAQNALQHVLGYTIANDVTARDLQQRDNQWTRAKGFDT
                     FCPIGPLIETELDTSDVLVTCRVNGEMRQMSSTKEMVFSIPQLIAFISSIMTLNPGDM
                     ILTGTPSGVGELKEGDLLESEIEGIGILRNAVTSQSSIPPP"
BASE COUNT         1904 a         2149 c         1937 g         2146 t
ORIGIN      
        1 cgaagggcgg tcctttaacc acaaaggaat taatgcagca aattgaattc aataccgttg
       61 aaaatcaaag gctggctgaa ttttctttaa atctcgcttt acaagaggat gaacgatacg
      121 acgaagtagg accatccggc gaagtgctct ggtatctgcg ctctatggaa ccggaggatg
      181 taaaaaagac tcccctcttt ttgcaatata aagagatgcc tgttgaaaat ccccaggtta
      241 accaatatct tgcattattt gaaggcacac tggcagacga gttagaagtt gatgaaccgt
      301 tgaattctga tgtaaccgtt acgatcagtt tgcttttccc ccactggcgc gcaggaaccc
      361 ttccattctc caatgcgcta tcccatatct tccccaccgc atttgaatct ccgcgcgttc
      421 gatttacttt tagggacgaa gataccgatg aaactttcaa cggttgggtg gtccgcaaac
      481 aacgctatgt gtttggcttg agagattggt acgaaaagaa gggtgtgatt cctggcagtt
      541 tcctgtcgct gaaaaagggt agcaaccctg gcgaaatagt gattcgtggt gaaaaaagtc
      601 gccaaaacaa agagtggata aaaacggtat tggtgggtgc tgataaagga atcgttttcg
      661 ccatgctcaa acatccaatt gagataagct tcaatgagcg gatgtccatt gccattccga
      721 atacagaagc gttggatgca atttggaaag agcgagaaaa attgaatcct gataaggttt
      781 ttcttcaaat tgcgcatgaa ctcgcgaaac tcaatcctca aggtcatatc cacgctcagg
      841 agatgtatgc ggcagtcaat gtcgtttttc gttgcccgcc ttccccggtg cttaacttcc
      901 ttctctccag ctctgcaatc cagcatctgg gagatctata ctttcatatc aaagaaggag
      961 aaggataaaa gcgcatgaac cctgccggtc atttcagctt ggtaaaacct acccttgaga
     1021 cgcctttcca tattgacttt gattggtgga tcgaacatga cgcaaactgg agggtttttc
     1081 tgctcgattt cttatgtgat gaacaccggg cattgtacgt cgaaaaagat caaaacattc
     1141 agatcgatgc gatcgatcct caaaccgctg aagtgaagag ggttgacggt ttattaagta
     1201 ccttagttga ccactgtgcc cagcaggaag gtttcatttc aaaaaatact acgctggtta
     1261 acatgatctt caaaatcttc ctggcaaatg acaacgcgcc attgaacgcg aaccaactgg
     1321 ctgaattgac cggcaaacca gccatgacca tcttgcgcac cttctctggc gttcaggtgt
     1381 tcaaagggat acgaccttta caagaataaa atagattctt gcggtataat ttttccgctg
     1441 ttaatacatg ccctcgtagc gcaattggac agcgcatcgg acttctaatc cggcggttgt
     1501 gagttcgagt ctcaccgggg gcgccttttc cttcaaatga tcgaactcaa taggctttcg
     1561 ctggtacctg tttacttgac ccctctgcca tgattttcat cttttattct tccgcacggg
     1621 cggaacccgt gatcagtacg gtttgcggtt ggtggtaaat ttggctagcc cacaattggc
     1681 aggcggccaa ttttgcgcgg ctcattgatg cctgatattg atcgcggcgt agctggttga
     1741 aagtgcgcag ctcccagcgc tccacgagat tttccatgtg ctggttcagg aaagctgcaa
     1801 tggcttgcaa gttttcctta tcagcggtgg catgggggat gagcggcgtg cggatccata
     1861 aagtgaagtg gtattcccta cccctgagta tgtccgctat ccacaacaaa ttcgccagaa
     1921 tccgctcatt gtcggcaccg gtaagctgtt tgtgctgttc tgcattcatc aatttcagat
     1981 cgtatagaag cagatcggta tatggcaaaa tcatttccag gttttcctga gggaccgctc
     2041 cgcaggtatt tagcacaatg tgaatgccct gttccttcag tattttcatc aaagcaatgc
     2101 agaattccgg ttgcaaagct ggctcaccgc ctgaaaaagt caccccacca cccgatctgg
     2161 cgaaggaagt tttatctctc aacaattctt cgctcaattc ctgcgcctca attttcttcc
     2221 agggcaactc gagagcattt gccgggcagg cttcggcaca ttttccgcac aggacacatc
     2281 gtgaataatc tatacgcagt cctctgtctt ccatagaaat acatccaccc gggcaggaaa
     2341 ggacgcaggt cttgcagccg atacagaaat gttcgagcca ccgaatgtgg gttgctacgc
     2401 gagcgatgct ttcagggctg cggcaccagg cacactgtaa ggtgcaaccc ttgagaaaga
     2461 ttgttgtacg ggtgcccggt ccatcttctg tagaaagacg ctgcaaacgg agataatttg
     2521 cagtaattgg ttcagtcttg atcatggcgc tttcccgtgt tatttcccat atggtgtgtc
     2581 acaggcgatg acatcattct gcaatatgat ttaaaaaatt ataacatttc ccattagcgg
     2641 tttttcaaca gcatgagtag ttaccgggga cgggaaatta ttaatacagg ttttacaatc
     2701 ctctgatgaa accttcgtat aattaaggta tagtttttca cataaatacg gaggaatgaa
     2761 gttatgtatg gctatggtct ttatttactg atatctttgc ccgctctgat cttgggtcta
     2821 tgggcacaag caaaggtaca gggagcatat aaaaagtatt cccaggtacg cacatcgaat
     2881 ggaatgaacg gcgctgatgt tgctcgtcat atcctggact ccaacgggct ctcgagtgtt
     2941 gcgataaaac agaccggcgg tacattaagc gataattatg atccccgcaa tagaaccctc
     3001 aacctcagca ctggggttta ccaaagcaac agcatcgcgg ctgccggtgt ggctgcgcat
     3061 gaagcggggc atgccattca gcacaaggaa tcatatggtc cgcttaaggt acgctctttg
     3121 atgatcccaa ccgtccaatt gggaagctgg ctaggaccta ttttgttctt cgtcggatat
     3181 ttcttctcca attacaatct ggcattgatc ggtgtgatct tgttcgcagg aattgctttg
     3241 ttctctattt tgaccatacc ggttgaattg gacgcttcaa agcgcgccaa agcgatcttg
     3301 gctagtgaag gcattctgta tggcggcgaa atggaaggcg taagccaggt gctggatgcc
     3361 gctgcctgga cgtatgtggc agccgccacg caagccattt ccaccgtact gtattatgtc
     3421 cttcttcttg gtggacgaag tagccgcaga agataggtgt ttttccttaa cttaaaatcg
     3481 aaaatcggtg caacaaaatg caccgatttt ttttctgtca cttcttacgt cacagggaga
     3541 gagcgtttaa gctcatccag cattcccaaa gcccgcgccg ccccttctgc ggtacaggtg
     3601 gttgggttat ccaccaggta agccgggatt cccaattctt tggtcagata gcgttctacc
     3661 ccgcgcaatt gcgtgccacc accgcatagc gccacaccgc gatccatgat atcagaaatc
     3721 aattccggtg gcgttttctc cagcgtccgt ctgaccagtt tggtcaccaa ctgcagtcct
     3781 tcctgcactg cttcaatgat atcttgcgtc cgcaatgtta cagaacgggg aagccccgtc
     3841 acctgatctt gcccctgcac ttccatttcg ctctcttcga tcccttcaat ggcagccccg
     3901 atgcgcatct tcacaatctc agcgctgaat ctggacagga tcaccccgta tgttttacga
     3961 acatgatcga tgatatcgag atctagtttg ttgccgcccc ctcgttcgat ctctgcggag
     4021 atgatgctgt tcatcgccag caccgccacc tggttggttc ctcctcccag gcagatgatc
     4081 atgtttccag aaggcggtgc gatcggcagg tcaataccca gagcggctgc cagtggcgcc
     4141 tcgatcaaca aaacctccct ccccccggct cccagcccag cctcatgcac tgcgcggcgt
     4201 tccacgctgg tgatgccgta aggaacggtc aacatcaggc tcggtctgaa cagcaacatc
     4261 gatcccacaa tcctgtggat ctggtatttc aggaaatttt ccgtgatctc atattctgcg
     4321 atcacgccat tttggattgg gaaataaacc tcaatcgaat cgggcacacg tccggtcata
     4381 tctttcgctg cctgcccgac ctcgatcatt ttccattcct caagcaggac cgacaccgcg
     4441 gtgggttcat gcagcaggat ctgcccattc tccgcaatca cggtattggt ggtgcccagg
     4501 tcaattccta atcctctaga aaaacttgcc attcaactta cctcaatgcg atctcaaacg
     4561 ctgaattcta acatacactt gctctaagcg gaaaagtatt gcagcgccaa acgcaacctt
     4621 tcttctgggg tttgtggagc atcacgtgga atggaagcca ccgctttttg cgcttcgtac
     4681 aatgaatagc ccagcccgac cagcgcctgc aacacatccg tgtcggtatc cgggatctct
     4741 tccaaaccag cttcttccaa taatggtgca atcttatctt tcagcatcaa gacgatcttc
     4801 ttggctgtct tttcgccaat gcccggaaca tggctgaaaa cttcatcttg ctccttcatc
     4861 actgcgcgct ggatggcttc agcggacaaa gaagataaaa ggttcaatcc cagtttcgga
     4921 ccgatcccgc tgacgctcag aatttgtatg aaaagatcgc gttcttcgat cttctcaaaa
     4981 ccgtacaaga acatgccatc ttcgcgggtg atgtgatggg tatacaaact gatatgatta
     5041 cccacctcag cctgtgcaca ggttgctttg gtgcaggata tcttgaaacc aatcccgccg
     5101 gtctctacca ccagcgaatg gtcattcttg aacgtaacaa ttcctttaag tgaagcgatc
     5161 atctctttct cagcgggtga tgctgattga aatattcata cagcagcacc gaggcggcat
     5221 tagccaggtt caatgaatcc actgtgccta tcatggggat ggtgaccaga gatgtacaca
     5281 cctgctgcag agatgcaggc aggccttttt gttcacttcc catgcacaga atcgtgttct
     5341 cagcataatt atagtcgcga taatcgtggt gtgcgtcaca aacagcccca atcaggtcga
     5401 ttgtttgctc ctgtttccac cggatgaatg tgggaacctc gcattggtaa agctgcgtgc
     5461 tgaagatcgc ccccgtgctg gcgcgcacgc tggtgggatg gaatgcatcg gtgccttccc
     5521 cgatcaggat cagtcccttg ccccccaccg catcgagggt acgcagaatg gagcccaggt
     5581 ttcccggatc ctgcacgcct tccaatgcca cccataaacc tgtcaccgtt gtggacagga
     5641 gcaaatcaga cacgttttgc cggacgaccg cgcacatccc ctgtgggttc tccttcaagc
     5701 tgatgctttc aaagaccgcg cgactgactt ccagcacagg gataccggct ttttcggctc
     5761 tctgcaaaag gctctttccg aaatcacttt ccagtaattc cgcgcaaaac accacttttt
     5821 caaccgatgc gcggttttcc agagcgctgg cgatagcgcg caacccttcc acgtaaaaca
     5881 agctgctctc tttgcgaaat ttccgctggt tgaggctacg aatttctttg atttgtgtgt
     5941 tggaggtgct ggtgatcatc tcaggtatca agcatggtca aacgtgcgct atgcgcatgg
     6001 caaatggcga ttgccagtgc gtcagctgcg tcatccggtt tggggatctc atgcaggttg
     6061 agcaacgctt tcaccatctg ctgcacctgc cctttctcag ccattccata acctgtcaca
     6121 gccagtttca cgtccatggg attgtactca tagactggaa tggagttctg cgaaagcgcc
     6181 agcatgatca ccccgcgcgc ctgccccact tgcagggcag tgcgcacatt gcgctgaaaa
     6241 tacagtttct ccaccgccgc acactgcggc tgatagcttt ccaggatttc agtcaatttc
     6301 tcatgcagaa cctgcaaacg ctggtcggta tccatcgcac tggaagtgcg gatggttcca
     6361 tgcgctaaca ggcgcagtga cctgtctggt agttcatcga tgatcccgta accggttatg
     6421 gctgttccgg ggtcaagccc gattaccagc atgggtcatt cttcttccat gctagccagg
     6481 gcttcgtcgc tgacctccag gttggaatac acgctttgca cgtcatccaa atcttccagg
     6541 tcttcgacca aacgcatgac cttcaccgtc tgttcgttat ccaatacgat ctcttgcttg
     6601 ggcaccatac gcagttctgc ttcatccact tcgatctttg cctctttcaa caggtcattg
     6661 atcgttttga atgcttccac cggcgcaaag atctcgatat atccttcttc atcctgcgtg
     6721 atatcattcg ccccaccctc gagtgccagt tcgaagacct tatcgaaatt cacaccctca
     6781 ccttcgatgc caaagtaagc gatcctgtcg aactgccacg agactgaacc cacctcaccc
     6841 atgttgccgc cagcgcgcgt taacacatgg cgaatctcag aaaccgtccg gttgcgattt
     6901 tcggtcaggc attccaccat tagtgcgatg ccgtgcgggg catatccttc gtaagtgatc
     6961 tgttcaatca cttcgccgtc tttgtcttcg ccggtgccgc gtttgatcgc tctgtcgatg
     7021 ttgtctttgg gcatgttcac agcgcgggcg cggtcgatgg caatgcgcag gcgaaagttt
     7081 ccttcaaggt cgctcccgct ctcacgtact gcaatgacaa tctcacgggt tatgcgggta
     7141 aaaagctgac cgcgtttagc atccgctgcg cctttttttc tttttatcgt agaccacttt
     7201 gagtgacctg acatggtatt ttctcctcgc ttttcaatcc tatgaaccaa ttattgcttc
     7261 tggaattaaa ttataacatt tcgggttcag gagcgatccc gcccgtgctt gagaaaactc
     7321 tagggtggag ggatgctgga ttgcgatgta accgcatttc gcaggatccc gatcccttcg
     7381 atctcagatt ccaacaggtc gccttctttc aattcgccca cacccgatgg ggtgccggtt
     7441 aagatcatat ctcctgggtt aagtgtcatg atggaggaaa tgaaagcgat cagttgaggg
     7501 atgctgaaca ccatttcttt ggtcgaagac atctggcgca tttcgccgtt cacccggcag
     7561 gtcaccagca cgtctgaggt atccaattct gtttcgatca gcggtccgat ggggcagaat
     7621 gtgtcaaagc ctttggcacg cgtccactgg ttgtcgcgct gctgcagatc gcgcgcggtg
     7681 acatcattgg caatggtgta accgagcacg tgctgcagcg cgttctgcgc tctgatccag
     7741 cgtccgccct tgccaagcac aactgccagt tccacttcat gctccacatg ctgtgattgg
     7801 ggtggaagct ggatcgcctg atccggcccg atcacagcgc tgagcggttt aagaaaaata
     7861 atggggatct cggggatctc gaccgattgt tcctgcgcgt gcaggggata gttgcgcccc
     7921 accgcgatga tcttgccagg caggatgggc ggcagcaggc gtactttttc caggtcatgt
     7981 tccaattcca agcgccggta caaacccaat ggagaagcgc tgagaggacc tacccttcct
     8041 tcagtttccc agccaaagcg gggctcttga tcggcaattt ggtagcgtac gatgcgcata
     8101 agattcctcc ctaattatat ctgggttttt catgga
//
LOCUS       DAOSDS010000093         8089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3298_length_8089_cov_4.020911, whole genome shotgun sequence.
ACCESSION   DAOSDS010000093 DAOSDS010000000
VERSION     DAOSDS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3298_length_8089_cov_4.020911"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PLL88_09410"
     CDS             complement(<1..1563)
                     /locus_tag="PLL88_09410"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein"
                     /protein_id="HOJ01821.1"
                     /translation="MDTIIGKPIPRFDAHDKVTGAAKYAADVNLPDQLFLKTIFSPSA
                     HAIIQSIDTSKAETSRGVVKVLTAKDVPCNEYGLLINDQPVLCGPGSAKIFSERVRCI
                     GDRVAFLIAESEEIAQQAAGLVKINYKDLPTITDSVQSKNLKKPLIHPELASDRCSHH
                     QVRKGDVQSAFRMADVIVEATYYTPPQEHAFLQPEAGIAFMDEENVIHIITAGQWAHG
                     DQRQVAHALGLAEDRIRIEYAAIGGAFGGKEDISVQIALGLAVLCLDQRGIRRPVKTV
                     WSREESFIGHHKRHAFQIYAKWGALRSGKIFAAEMRLIADAGAYASSTDVVLKSATIA
                     AIGPYDIPNVCIDADAFYTNNIPAGAFRGFGVPQACFAAEMQINKLAQALQIDPVKLR
                     LENAIQEGISSLNQTPLPPGIGITEVIEKCADAVGWKENGSGYQGNKNPGRYVYGWGF
                     AAGFKSFGIAPDECWAKVELIGGQEIEQVKIYHAAADMGQGVHSVLKQFAAETLAVPV
                     EQINLIVSDTASS"
     gene            complement(1624..2964)
                     /locus_tag="PLL88_09415"
     CDS             complement(1624..2964)
                     /locus_tag="PLL88_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOJ01822.1"
                     /translation="MTIYVIGHVNPDTDSIASAMGYAWLLNERDGTEAIAARSGVINP
                     QTSWVLDFLHLKPPFLLNDASPKFGSVAIRMDTTTPDKPLWEAWGIASRTGGVAPVVT
                     EDNHPFGLVTGASLFKLMAEFVGPNARSSNMAVNQFLELPCREAADTTVQHFNSSTRI
                     KDLIRKVLREEGDDFWVVDDNGKYAGIARKSDLIHPPRIKLILVDHNEAQQAVSSFEE
                     AELIEILDHHRLGNMPTNSPIHFTVVPVGSTSTLVTERIAEAGLAAPPEIAGVLLAGI
                     ISDTLNLISPTTTERDREAVKRLSRWAFISDSPLAKETIDTYAKNVLNSGSGLSARPP
                     REIINNDVKSYEVGEYKFSISQVEVSDLYELDEHIDDLQTALEEYRESKGNHFAVLMV
                     TDIVRGSSRVLMVNPPAMLEDLPFNPDSDGTLFAEGMVSRKKQLVPIILSQLEV"
     gene            complement(2990..3985)
                     /locus_tag="PLL88_09420"
     CDS             complement(2990..3985)
                     /locus_tag="PLL88_09420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HOJ01823.1"
                     /translation="MKDRVALYLQDAHDLRDGLDYVKYAEQKGFEAVWQAESRLVRDA
                     IVPMAAYAAVTERLKIGSGVINNWTRNIGLLASTFLTLDDLAPDRIICGIGAWWDPLA
                     KNVGIDRSKPLSAMKETVTVLKRLLNMERVSFDGEFIHVKNIELDVVHGRKEPRHVPV
                     YIGATGDRMLEMSGEIADGVVLNYCVPPEYNLKALELIEKGAKKAGRELEQLDLPQLV
                     VCSVDYDHDKAIDTTRELLTQYLAQQPHIAKASGVSMEVVQEIQSILGWPATHEQIQK
                     AKHLVPEELIHRITASGTPQEARAKVEEYKRNGCSCPILYPVGGDVHLLIDTFAE"
     gene            complement(4008..4613)
                     /gene="cofC"
                     /locus_tag="PLL88_09425"
     CDS             complement(4008..4613)
                     /gene="cofC"
                     /locus_tag="PLL88_09425"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HOJ01824.1"
                     /translation="MSLWAIVPVKSLRRGKSRLASVLTDEERSTLNTDLLIRTLHCLK
                     SIPKIDQVLVISYDPEVLARAREFDAMTVQEGKRTNLNNALRRATVAARAYNATKILV
                     IPADLPYISKEGVEDFIAQEGTPPEIIISSDQRSNGTNALFVNPIGILEYNFGEWSFK
                     KHIEQAERKRIHVKVANKESLTFDLDLPEDLEIYLKACKIN"
     gene            complement(4623..5567)
                     /gene="cofD"
                     /locus_tag="PLL88_09430"
     CDS             complement(4623..5567)
                     /gene="cofD"
                     /locus_tag="PLL88_09430"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HOJ01825.1"
                     /translation="MKKTMRIVALAGGVGGAKLADGLAQCLKPSELAVIVNTGDDFEH
                     FGLAISPDLDTVCYALANIANPDTGWGRKDETWRTLNEIKKLQGADWFNLGDLDLATH
                     LERTRRLKEGQTLSTITAHFCAFWGIKVPVFPMSDTPVRTFVHCKDGNILPFQEYFVK
                     HRFQPQIDRIELKNIEQAVVPEKAIAALQEADCIVICPSNPFVSIDPIIKIKAIADIL
                     SQKKVLAVSPLIGGEALKGPAAKMFREMGIPSSSSEVARHYQDILSLFILDHRDRSQI
                     AVLQGWGIMCKESDIYMPDRASRKRLAGEILKFITENR"
     gene            complement(5564..6340)
                     /gene="cofE"
                     /locus_tag="PLL88_09435"
     CDS             complement(5564..6340)
                     /gene="cofE"
                     /locus_tag="PLL88_09435"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOJ01826.1"
                     /translation="MPRSPLILTPINTLPLIEPNQDLAELTFHALEEAQIHLEDDDIL
                     VITQKVVSKAEGRLVNLSTVFPSQRAWEVAAITQKDARLVELILSESKTIIRAQANTL
                     IVEHRLGFICANAGIDRSNVKGVWGNEEDWYLLLPQDPSASALKIRDLLLAKTRKSVG
                     VLIIDSHGRAWRRGTVGITIGLSGVPGIIDMRGKEDLFHYHLKVTQIAVADELAGSAS
                     LMMGQADERTPIVHVRGFPYPLVDTPFQEILRNKEEDLFR"
     gene            complement(6351..7001)
                     /gene="npdG"
                     /locus_tag="PLL88_09440"
     CDS             complement(6351..7001)
                     /gene="npdG"
                     /locus_tag="PLL88_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HOJ01827.1"
                     /translation="MLIAIIGGTGKEGKGLAYRWAKAGHQIIIGSREYEKAQTVASEL
                     NRIFKGIEPIRGDQNVEAARKAELVVITIPYSAHAPILESIRDVVQNKIVIDVTVPLI
                     PPRVTHVQIPTDGCAAMEAQKILGDSVQVVSAFQNVSYEKLLDDEELHCDVLVCGNDK
                     SAKQIVLALIKDMHLVGYDAGPLQNSIVSEGLTSVLININKQFGIQNAGIKITGIP"
     gene            complement(7026..>8089)
                     /locus_tag="PLL88_09445"
     CDS             complement(7026..>8089)
                     /locus_tag="PLL88_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOJ01828.1"
                     /translation="RLDKALDHEAIYYSALVCMIDAIRHGTTTLIDHHASPNAISGSL
                     DDIAQAALESGLRVSTCYEVTDRDGKEKARAGIEENIRFIRRYEKAELPERIFPTFGL
                     HASLTLDDDTLETCASAIPQGYGFHIHVAEHQVDEFDSIFRSGKRTIERLDAFRILNP
                     LTIVVHAVHIDAREMQIIKEREAWVSHQPRSNMNNAVGLPQVEGMLNLGIPVCLGNDG
                     FSNAMWDEWKAAYLAHKLWNRDPRRMGAASVAKMAWVNNAKLVQKLSGSEVGVIKEGA
                     KADLILVDYKPFTPLSVENLPWQLVFGFQNSMVTTTIVDGKVLMRNGAITVLDEEQVM
                     RAALQLAPKVWERYAQQFS"
BASE COUNT         1883 a         2123 c         1864 g         2219 t
ORIGIN      
        1 tgaagatgct gtgtcgctga caatcagatt gatctgttca accggaaccg cgagggtttc
       61 cgctgcaaat tgcttcaaaa ctgaatggac gccctgcccc atgtcggctg cggcatgata
      121 aatcttcacc tgttcaattt cttgtcctcc aatcagttcc acttttgccc aacattcatc
      181 aggtgcgatg ccaaaagatt taaacccggc agcgaatccc cagccgtaaa cataacgtcc
      241 cgggtttttg tttccctgat accccgaccc attttccttc cagccaactg catcagcgca
      301 tttttcgatc acttccgtga tgccaatgcc gggtggcagc ggggtttggt tcaaggagct
      361 gataccttct tggattgcat tttctaaacg caatttcacc gggtcgattt gcagtgcctg
      421 cgctaattta ttgatttgca tttccgccgc aaaacatgcc tgtggcacgc caaaaccacg
      481 gaacgctccc gccgggatat tattggtata gaatgcatca gcatcaatgc agacattggg
      541 gatatcataa ggtccgatcg ctgcaatagt ggcgcttttt aacaccacat cggttgacga
      601 tgcataagcg cccgcatcgg caatcaagcg catttctgcg gcgaagatct ttccagaccg
      661 caacgcaccc cacttggcat aaatctgaaa ggcgtgccgt ttatgatgcc cgatgaagga
      721 ctcttctcgg ctccacaccg ttttgaccgg gcgacgaatt cccctttggt ctagacagag
      781 aacagccagt ccaagcgcta tttgaacgga aatatcctct tttccgccaa aagcaccccc
      841 tatggcagca tattcaatac ggatgcgatc ttccgccagt cccaaggcgt gggctacctg
      901 gcgctgatct ccatgtgccc attgccctgc cgtaatgata tggatgacgt tctcttcatc
      961 cataaaagcg atccccgctt ctggctgcag gaaggcatgt tcttgtgggg gcgtgtaata
     1021 cgtagcttca acgatcacgt ccgccatgcg gaacgctgac tgcacatccc ctttgcgcac
     1081 ctgatgatgg ctgcagcggt cgcttgccaa ttccggatgg atcagaggct tttttaagtt
     1141 cttgctttgt acggaatccg tgatggtggg caggtctttg tagttgatct taacgagccc
     1201 tgcagcctgt tgggcgatct cttcgctttc tgcgatgagg aatgccaccc gatccccaat
     1261 gcagcgcacc cgctccgaga atatctttgc tgatccgggt ccgcataata cgggttgatc
     1321 attgatcaga agaccatatt cgttacacgg tacatctttg gcagtgagca ccttcaccac
     1381 tcccctgctt gtttcggctt tgctggtatc aatgctctgg ataatggcat gagcagacgg
     1441 agagaagatc gttttcagga ataattgatc agggaggtta acatcagcgg catatttcgc
     1501 agcaccggtg actttatcgt gtgcatcgaa gcgggggatt ggtttaccaa taatcgtatc
     1561 catattcctt cacaaataat aaaaatgatc tgccgaaatc gcttcaacag atcattttca
     1621 cttctaaacc tctaattggc tgagaatgat gggcaccagt tgcttctttc gcgagaccat
     1681 gccttccgcg aacaaggttc catctgaatc aggattgaac ggaaggtctt ccagcatcgc
     1741 gggtggattg accatcaaaa cgcggctaga cccacgcacg atatcagtca ccatgagcac
     1801 cgcaaaatga tttcctttgc tttcccgata ctcctcaaga gccgtttgaa gatcatcgat
     1861 gtgctcatct aattcgtata agtcactaac ttcaacttgc gatatcgaga atttatattc
     1921 acccacttcg taagacttca cgtcgttatt gatgatttcc cgtggcggtc gggcactcag
     1981 acctgaacct gaattcaaca cgttttttgc ataggtgtct atggtttctt ttgccagagg
     2041 ggaatcactg ataaatgccc accttgataa tcgcttcacc gcctccctat cccgctcggt
     2101 tgttgttgga gagatcaggt tcagggtatc tgaaatgatg cccgccagca agaccccggc
     2161 gatttcgggt ggtgccgcca accctgcttc ggcaatgcgt tctgtcacca gcgtggatgt
     2221 gctgccgacc ggaacaacgg taaagtggat gggagaattg gtcggcatgt tgcccagacg
     2281 atggtggtcg agtatctcga tcaattctgc ttcttcaaat gatgaaacgg cttgctgcgc
     2341 ttcattgtga tcaacgagaa tgagcttgat acgcggcgga tggatcaggt cgctcttgcg
     2401 cgcaattccg gcgtattttc cgttatcatc caccacccag aaatcgtcac cctcttcgcg
     2461 cagaactttc ctgatcaaat ccttgattct cgtggatgaa ttgaaatgct gaacagtcgt
     2521 atcggctgct tcgcggcagg gtaattccag aaactggttc acagccatgt tgctgctgcg
     2581 cgcgttcgga ccaacgaatt cggccatcaa cttgaataaa ctcgctcccg tgaccaaccc
     2641 gaatgggtgg ttatcttccg ttacaacggg agcaactccg cccgttctgc tggcgattcc
     2701 ccaggcttcc cacaagggtt tgtcgggagt tgttgtatcc attcgaattg ccactgaacc
     2761 aaacttcgga gaagcatcgt tcagaagaaa tggcggtttc aggtgtaaaa aatcgagcac
     2821 ccaggatgtt tgagggttga tcacccccga tcgggcagct attgcttcgg ttccatcacg
     2881 ttcgttgagc aaccaggcat accccatcgc agaagcaata gaatccgtat ccggatttac
     2941 gtgaccgatt acatagattg tcataagtgt ttcctcccaa acagcgcagc tattctgcga
     3001 aggtatctat cagcaaatgg acatctccac ctaccgggta caggatcggg cagctacagc
     3061 cgttgcgctt atattcttcc acctttgctc tggcttcttg cggtgttcca gaagccgtaa
     3121 tgcggtggat caattcttct ggaacaagat gcttggcttt ttgaatctgt tcgtgggttg
     3181 ctggccagcc tagaatcgac tggatttcct gcactacctc catggaaacg ccggatgcct
     3241 tcgcaatatg aggctgttga gccaggtatt gcgtcagcaa ttctcgggtg gtatcaatgg
     3301 ctttatcgtg gtcatagtcc accgaacata ccactaattg aggaaggtcc agttgttcga
     3361 gttccctgcc ggctttcttt gcgccttttt cgatgagttc caacgctttc aggttgtatt
     3421 cgggaggcac acagtaattc aacaccacgc catcggcgat ctcgccgctc atttcgagca
     3481 tcctatcgcc cgttgctccg atatagactg gcacatggcg cggttctttg cgtccgtgaa
     3541 ctacatctaa ttcaatattc ttcacatgaa tgaattcgcc gtcaaaactg acccgctcca
     3601 tattcagcaa ccgtttgagc acggtaacgg tttctttcat ggctgacaat ggtttgctgc
     3661 gatcaatccc cacattcttg gctagcggat cccaccaggc gccaattccg caaatgatcc
     3721 ggtcgggcgc cagatcatcc agggtaagaa aagtggatgc cagcaagcca atgttcctgg
     3781 tccagttatt tatgacaccc gatccaattt ttagtctttc cgtcactgcg gcatatgccg
     3841 ccatggggac gattgcgtcg cgcacgagac ggctttctgc ctgccagact gcttcaaagc
     3901 ccttttgttc agcgtatttg acataatcca gtccatctcg taaatcatgt gcgtcctgta
     3961 agtacagtgc aactctgtct ttcacggtaa cctccttgga tcggttgtta atttatttta
     4021 caggctttca gatagatttc caaatcttca ggcaaatcca ggtcaaatgt gagagattct
     4081 ttgtttgcta cctttacatg aatccttttt ctctccgctt gttcaatgtg tttcttaaat
     4141 gaccattccc caaagttata ttccagaatc ccaatggggt tgacaaaaag cgcgttggtg
     4201 ccattcgagc gctggtcaga agagatgatg atctctggcg gtgttccctc ttgcgctatg
     4261 aaatcctcta ctccttcctt actgatataa ggaagatcgg cggggatgac caatattttg
     4321 gtggcgttat acgcccttgc agccactgtt gccctgcgta gcgcgttgtt gagattggta
     4381 cgtttgccct cctgaacggt catggcgtca aactcgcgcg ctcttgcgag aacttcagga
     4441 tcataactga tcaccaacac ctggtcgatc ttggggatgg atttaaggca atgtagtgtt
     4501 cgaattaaaa gatcagtgtt aagcgtagag cgctcttcat ccgtcagcac actcgccagg
     4561 cgggactttc ctcttcgtag agatttcacc ggaacaatcg cccaaagcga catgattgat
     4621 ctttaacgat tctctgtaat aaatttcagg atctcacctg ccaatcgctt gcgggacgcg
     4681 cggtcaggca tgtaaatatc cgattccttg cacattatac cccacccttg tagcaccgcg
     4741 atctgactcc tgtctctgtg gtccagaatg aacagggaaa gaatatcttg ataatgtctt
     4801 gcgacttcac tggatgaaga aggtatcccc atctccctga acattttggc tgcaggtcct
     4861 tttaatgctt ctcctcctat taatggcgaa actgccagaa ctttcttttg ggaaagaata
     4921 tctgcaattg ctttaatctt gatgatcgga tcgatgctta caaagggatt ggaggggcag
     4981 atcacaatgc aatctgcttc ttgcaacgcg gctattgcct tttctggaac gaccgcctgt
     5041 tcgatatttt tcagttcgat cctgtcaatc tgcggctgaa agcggtgttt tacgaaatat
     5101 tcctgaaatg gaaggatgtt cccatcttta cagtgcacaa acgttcgcac aggcgtgtca
     5161 ctcatcggaa aaaccggtac cttaattccc cagaatgcgc agaaatgtgc tgtgatcgtg
     5221 gagagcgttt gcccttcttt cagccttcgt gttcgttcta gatgggttgc caaatccaga
     5281 tcgcccaggt tgaaccagtc tgcgccttga agtttcttga tctcattcag ggtgcgccag
     5341 gtttcatctt ttctgcccca gccggtgtca gggtttgcga tatttgccaa tgcatagcac
     5401 accgtatcca ggtcgggtga aatggcaagc ccaaagtgct cgaaatcatc ccccgtgttt
     5461 acaatgaccg ccaattcact tggcttcaga cactgcgcca gaccatctgc cagttttgcc
     5521 ccgcctactc cccctgctaa tgctacaatg cgcattgttt ttttcatcgg aacaggtctt
     5581 cttctttatt tcttaaaatc tcctggaaag gcgtatcgac cagcggatag ggaaaacctc
     5641 gcacgtgtac tatgggggtc ctttcatccg cctgacccat cataagcgaa gctgacccag
     5701 ccaactcatc agcaactgcg atctgagtaa cctttaaatg atagtgaaaa agatcttctt
     5761 ttcctctcat atcgataatc cctggcacgc ctgataaacc gatcgtgatc cccaccgtgc
     5821 ccctgcgcca cgcgcgccca tgcgaatcga tgatcagcac cccgacgctt tttctagtct
     5881 ttgccaataa aagatccctt atttttaacg ccgaagcgct ggggtcttga ggcagaagca
     5941 ggtaccaatc ttcttcattt ccccagactc ctttgacatt agaacggtcg atccccgcat
     6001 ttgcacaaat gaaccccaag cgatgttcca cgatcagcgt attggcttgc gcgcgtatga
     6061 ttgttttgct ttctgacaat atcagttcta ccaaacgggc atctttttgc gtgattgcag
     6121 ccacctccca agcgcgttgg gaggggaaaa cagttgaaag gttcaccaac cttccttcag
     6181 ctttggatac gaccttttga gtgatgacaa ggatatcatc atcctccaaa tgaatttgtg
     6241 cttcctccaa agcatgaaaa gtaagctctg ccagatcttg gttcggttcg atcagtggga
     6301 gggtgttaat gggcgtaagg attaaagggc ttctcggcat atgctcaatt tcatgggata
     6361 ccagtgattt taatccccgc gttctgaatg ccgaattgct tattgatatt gatcaataca
     6421 gaagtcagtc cttcgcttac aatggagttt tgcagcggac cggcatcata accgaccaga
     6481 tgcatatcct tgatcaacgc cagcacgatt tgtttcgcac ttttgtcatt cccgcaaacc
     6541 aaaacatcac agtgcagttc ttcatcgtcg aggagttttt cgtacgagac attttgaaaa
     6601 gcggaaacta cttgcacgct atcacccagt atcttttgag cttccatcgc cgcgcaaccg
     6661 tcagttggga tctgcacatg ggtcaccctg ggggggatca atggcacggt tacatcgatc
     6721 acgattttgt tttgcacaac atcacggatg gattccagaa ttggcgcatg ggcagaatat
     6781 gggatagtga taaccaccag ttcggctttt ctggcggctt caacgttctg gtcaccacgg
     6841 atcggctcaa tccctttaaa tatcctgttc aattccgaag cgacagtttg cgctttctcg
     6901 tactcgcgag agccaatgat gatttgatgc ccggcttttg cccagcgata tgccagtccc
     6961 ttcccttctt ttccagttcc acctatgatg gcgatgagca tgaagaacct cctgttgggt
     7021 aacctttatg aaaattgttg tgcatatcgt tcccagacct tgggggcaag ctgcaatgca
     7081 gctcgcataa cctgctcttc atcaaggact gtgattgcac cattccgcat caatactttt
     7141 ccatcgacga tggttgtagt gaccatgcta ttctgaaagc caaaaacaag ctgccatggc
     7201 agattctcta ctgaaagcgg ggtgaaaggt ttgtagtcaa ccagaatcag atctgctttt
     7261 gccccttctt tgattacacc aacctcagaa ccgctgagtt tttgaaccag ttttgcatta
     7321 ttgacccaag ccattttggc tacgctagct gcccccatac ggcgcggatc gcggttccat
     7381 aacttatgag ccagataagc ggctttccat tcatcccaca tggcgttgga aaacccatca
     7441 tttcccaggc acaccggaat gcccagattc agcataccct caacctgtgg tagtcccaca
     7501 gcattgttca tattggagcg cggttgatgg gagacccatg cttctctttc ttttatgatc
     7561 tgcatctccc gggcatcgat gtgcaccgca tgcacaacga tggtgagggg attgaggatc
     7621 cgaaaggcat ccagacgttc aatagttcgt tttccgctgc gaaatatgct gtcaaactca
     7681 tccacctggt gttccgcgac atgaatatgg aatccatagc cttgaggaat tgcggatgcg
     7741 caggtttcga gtgtatcgtc atcaagcgtc aaactggcgt gcaagccaaa agtgggaaaa
     7801 attctttcag gaagctctgc cttctcgtaa cgacggataa aacggatgtt ttcttcgatc
     7861 cctgccctgg ctttctcctt tccatcccga tcggttactt cgtaacaagt ggatacgcgc
     7921 aacccacttt ccagtgctgc ctgggcaata tcatccagcg acccgctgat cgcattcgga
     7981 gacgcgtggt ggtcaatcag ggtcgttgta ccatgccgaa tggcatcgat catgcaaact
     8041 aaagcggaat aatagattgc ctcatgatcc agggctttat cgagtctcc
//
LOCUS       DAOSDS010000094         7814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3432_length_7814_cov_4.506122, whole genome shotgun sequence.
ACCESSION   DAOSDS010000094 DAOSDS010000000
VERSION     DAOSDS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7814
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3432_length_7814_cov_4.506122"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /gene="cgtA"
                     /locus_tag="PLL88_09450"
     CDS             <1..903
                     /gene="cgtA"
                     /locus_tag="PLL88_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HOJ01829.1"
                     /translation="GNARFASSRHQAPRMAERGEPGQELDIRLELKLIADVGIIGMPN
                     AGKSSLLAAVTNAKPKIADYPFTTLVPNLGVCLLDKEITLVLADIPGLIEGAHLGVGL
                     GDAFLRHIQRTKVLVHLLDGNSTDAMADYSQINSELSLFDPRLGEKPQVVVLNKMDLP
                     GMEEKYRVLKEQFKAIDVALFSCSTLQKKNLREVLWRAHELLAAIPAAPTEEMPLYTA
                     GVDEKAFFVERGEDAFIVRGIAIERAAAMTYWDNYESIRRFQRILQALGITEALREEG
                     IVEGDTVEIGEYELTWTEEFGDYE"
     gene            918..1535
                     /gene="nadD"
                     /locus_tag="PLL88_09455"
     CDS             918..1535
                     /gene="nadD"
                     /locus_tag="PLL88_09455"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOJ01830.1"
                     /translation="MRLGIFGGTFDPPHVGHLILAMEAYDQLQLDKVLWIIAPNPPHK
                     LGKPITPIDQRIAMVKTAIDGDAMFVLSRIDIDRPGPHYVLDTMLLLHASYPADSLVF
                     IMGGDSLHDLPNWHEPQKFIDQCSSIGVMRRPGEEIDLTLLKTQLPGIEKKVEFIDAP
                     LLEISSNKIRALAYQKKPFRYYLPAGVYDQIVELGLYNTEDALKE"
     gene            1538..2173
                     /locus_tag="PLL88_09460"
     CDS             1538..2173
                     /locus_tag="PLL88_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01831.1"
                     /translation="MKKVLLVLGLLLFFCSACVLLEEFPIIVIVTETSTSQSTAVTRT
                     PTIIPSSTVGITPTKTATIMPTATKTPSPSPTPQPSATPTAVPYQLQSGSPAYTANFG
                     YPEAGCNWLGVGGQIFNENGNPIINLVVWIRGKINGTPFESVVLTGTAEGDKYGPGGY
                     EAVIANTVLESSGVFSIQILDLSGNVLTDPISFDTHANCGQNLILINFTKK"
     gene            2197..2970
                     /locus_tag="PLL88_09465"
     CDS             2197..2970
                     /locus_tag="PLL88_09465"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOJ01832.1"
                     /translation="MSSSDGFILMDFVKQLFSNWKRVALMMVIGGLLGMGISKVMPPV
                     YESKAVIAVTIDYTRTGALSDIQEDQAMRGVGSVIDSDLVREKVRQAAEEAGVAFDQD
                     SIVKNFTVERGDFRWLLRVRDNQAERATFLANLWAEQAMSTLDSAMEHAIIANHYQQY
                     LDTLEYCLQRLAPEGTSSEPCAKINFEFLQSEVEKTSSAIRDEQALSFGLMPTLQFFL
                     AEEAPLNTAAVRSTRGILIFSGALLGFLIAIIIPNKEKE"
     gene            2971..4440
                     /locus_tag="PLL88_09470"
     CDS             2971..4440
                     /locus_tag="PLL88_09470"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOJ01833.1"
                     /translation="MDERKDLWLIAQKVADILWIIMLALLPITSMPAIARLLGSDTVA
                     APSILVVFCLLLIWFIPYLLRRGRMVKESIPLLVFVIAALVSTLLANFQVIPPYKDIP
                     YLSNSVEAFMTLLIGVVFFLVTSSYLRDEKKAILTLRVINWSAMVMFLWTACQVVAWH
                     GFGHYPAWMFDLQGLISSRVLYRQRVNGLALEPSWFAHQLNMVYLPFWLAATIKGFSA
                     FKKRLGFLTVENILLMIGIASLFLTLSRVGWLGFLLMFTFLLVKLHSYLVRRVSAALE
                     KKQAQVARKHEKRLRIGISIFFMIVYVLIIAIALYVFSKIDPRMESLFDFSVGKDNPI
                     LAYVSELSFGERVVYWLAGWNVFSEHPLFGVGLGNAGFYFPKAITPYGWTLIEVRHLT
                     YRTSILLNVKSLWARLLAETGLVGFAIFCAWLYSLGRKFVQKTEIKGSIQGVCGLSGV
                     FVLCALLAEGFSIDSFAMPYMWVALGLAAADLALPAREG"
     gene            4445..6352
                     /locus_tag="PLL88_09475"
     CDS             4445..6352
                     /locus_tag="PLL88_09475"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HOJ01834.1"
                     /translation="MNKKVLLIFGGILLLILAAGIYLTGHKILPTQNLDVSSAQENLN
                     VLMQTEIPENDAYAIAAKYKNIHLDPHEPAHEKITYALGDTRQFYILDMVTNAYTAIN
                     ATLQYSTAHLYFWIEENVDFAREDVAKLCEEFEGQIYPLNREFFGSEKTPGIDDDEHL
                     FIVYTTKMTGALGYFSSADTLPQAIEPYSNEAEIFMLSASYAHLDDESTYGVLAHEFQ
                     HMIHQNLDANESTWINEGFSELAVLLNGYDPGGTDWLYARNPDIQLNFWPRDEVESTL
                     PHYGASFIFMTYLYDRFGEEFSRRLVADPANDLASIDEVLTDLQKRSDGERLNADDVF
                     QDWSIANLLQDTAIEDGRYGYKSLPELSSFRVDGKIDCTTAVKNPFTVRQYGVDYFSV
                     KCDHPLTIQFNGERSIPVVPADPHSGEYYFWSNKADQSDMTLTQTFNFRNVEGPIILE
                     YFVWYDLETDWDYVYLLASENGKEWLQLKPARCTEENISGSNLGCGYNGASRGWVVEN
                     VDLTKYAGKTVTLQFEYLTDASLTGEGFLLDDVRIESIGYETDFEEDEGGWVAKGFVR
                     MMNSLPQTYRVAIINKSVEETTVRRYTVLGNEPLTLDLNATTNEEIFLVVSGTARYTE
                     MPASYTIELLD"
     gene            complement(6361..6573)
                     /locus_tag="PLL88_09480"
     CDS             complement(6361..6573)
                     /locus_tag="PLL88_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HOJ01835.1"
                     /translation="MSRSSSFVAGALLGAAIGSIVALLYAPKSGEELRKEIQEKTGQI
                     TLEVKKAAAQKKRELEEEINSFKAQE"
     gene            6654..>7814
                     /locus_tag="PLL88_09485"
     CDS             6654..>7814
                     /locus_tag="PLL88_09485"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HOJ01836.1"
                     /translation="MFRKYKERGFDALASCSLLIMVFLSAYSLESTHWTEDLNMVTSL
                     ACIGVILGIALGVTVFQKRHIMILSTLYSLILLFLFLVVIPSGGNSWSEGWRMVYGRT
                     HMALRALLQGQPVPDAILFIITAGILYWTVSLWAGFALIRKANPWVPLGVQGASLVAT
                     QFFQPVLYRSSLLTGTFFFLFLFLIGRLNFLIAHSRWKERKAYEDQESGTIFLRTTAI
                     LSSVLVLLAWGVPFLMDVATPGSKQHKAFIQTIEEGEDFFSDLFFSFRTQPIQTETVF
                     GDTFDLGSAQPLGEEVLFTAVAPSSDFFDGNYYWKARSYSTYENGRWSSIDVEGKTID
                     ANTRLELAGATENEIGTFTMVVNAELTYFYLPGNTISINRDVRITEMFAGDED"
BASE COUNT         1900 a         1814 c         2070 g         2030 t
ORIGIN      
        1 gggaatgcgc gctttgccag ttcccgccac caggctccgc gcatggcgga aagaggagaa
       61 cccggtcagg aattggacat ccgattagaa ttgaagctga tcgctgatgt tggcattatt
      121 ggcatgccga atgcgggaaa gtcaagcctg ctggcggcgg ttacgaatgc caaaccgaag
      181 atcgccgatt acccgttcac aactttggtt cccaacctgg gagtttgttt gctggataag
      241 gagatcaccc tggtgctggc ggatatcccc ggattgatcg agggggcgca cctgggagtt
      301 ggattagggg acgcattctt gcggcacatc cagcgcacga aagtgttggt tcatctgctg
      361 gatggaaatt ccacagacgc catggctgat tattcacaga tcaacagcga actttcgctt
      421 tttgacccgc gacttgggga aaaaccgcag gtggtggttt tgaataaaat ggaccttcca
      481 ggtatggaag aaaaatatcg cgtgctgaaa gagcagttca aggcgatcga cgtcgcgtta
      541 ttttcctgct ccaccctgca gaaaaagaat ctgcgggagg tcttgtggag agcgcatgag
      601 ctgttggctg ccatccctgc cgcaccaaca gaggagatgc cgctctacac agccggagtg
      661 gatgaaaaag cgttcttcgt tgaacggggg gaagacgctt tcatcgtgag aggaattgcg
      721 attgaacgtg ccgcagccat gacctattgg gataattatg agtccatccg ccgtttccag
      781 cgcatcctgc aagccctggg gatcacagaa gccctgcgcg aggaagggat tgtggaaggg
      841 gataccgttg aaatcggtga atatgaacta acctggacag aggaatttgg cgattacgag
      901 taaaggaaaa cactacaatg cgattgggaa tctttggcgg cacgtttgac ccgccccatg
      961 tagggcatct gatcttggcg atggaagcgt atgaccagtt gcagctggac aaggttttgt
     1021 ggatcattgc tcccaatccc ccccacaagc tgggaaaacc gatcacgccg atcgaccagc
     1081 gcattgccat ggtaaaaacg gcgatcgacg gtgatgcgat gttcgtgctt tcgcgcatcg
     1141 atattgaccg cccgggtcca cactacgtgt tggataccat gctgctgcta catgccagct
     1201 atccagccga cagcctggtc ttcatcatgg gcggtgattc gttgcatgac ctgccaaact
     1261 ggcatgagcc acaaaagttc atcgaccaat gttccagcat tggcgtcatg cgccgaccag
     1321 gggaggaaat tgatctcacg ctgctcaaaa cgcagttgcc aggaattgaa aagaaggttg
     1381 aattcatcga tgcccctttg ttggagatct catcaaacaa gattcgcgct ttggcgtatc
     1441 aaaaaaagcc gtttcgctat tatttgccgg cgggagttta cgatcaaatc gttgaattgg
     1501 gattatataa tacagaagac gcattaaagg agtaacgatg aaaaaagtgc tgctggttct
     1561 gggactgttg cttttcttct gttctgcctg tgttttgctt gaggaatttc ctatcattgt
     1621 tattgttacc gagaccagca cgtcgcaatc caccgctgtc acaagaactc caacgatcat
     1681 acccagttcc actgtgggga tcacaccgac caagacagcc acaatcatgc ctaccgcaac
     1741 caagacccca agcccttcgc ccacgccgca gccgtctgca acccctactg ctgtgccata
     1801 tcaattgcaa agcggttcgc cggcatacac tgccaatttc ggttatccgg aagccggttg
     1861 caactggttg ggggtggggg ggcagatctt caatgaaaat ggtaatccga tcatcaatct
     1921 ggtggtctgg atacgaggaa agatcaatgg aactcccttt gaatccgttg tgttgactgg
     1981 cacggctgag ggggataaat acggaccggg agggtatgaa gcggtcatcg ctaatacggt
     2041 cctggaaagc agcggcgtct tctcgattca gattctcgac ctgagcggca atgttctaac
     2101 tgacccgata tcctttgaca cacacgcaaa ttgtggccag aacctgatct tgatcaactt
     2161 cacgaaaaag tgaatttcta ttcgaagagg attgttatga gctctagtga tggctttatc
     2221 ttaatggact tcgtcaaaca acttttcagc aactggaagc gagtggcgct catgatggtg
     2281 atcggcggtt tgctgggaat gggcatcagc aaggttatgc cgcccgttta tgaaagcaaa
     2341 gcggtgatcg cagtgaccat tgattacaca cgcacgggag ctctttcgga tatccaagaa
     2401 gatcaggcga tgcgaggtgt gggaagtgtg atcgattcgg acctcgttcg cgaaaaggtc
     2461 aggcaagcgg cggaagaagc cggtgttgca ttcgatcagg acagcatagt aaaaaatttc
     2521 acggtggagc ggggagattt ccgctggctg ttgcgggtga gagataacca ggctgaaaga
     2581 gcgacttttc tggcaaacct atgggcggaa caggcaatga gcacgctcga cagcgccatg
     2641 gaacatgcca ttatcgctaa ccattaccag caatatctgg acacgttgga atactgcctg
     2701 caacggctgg cgccagaggg aaccagcagc gaaccctgcg cgaaaatcaa ctttgaattt
     2761 ctgcagagcg aggtcgaaaa aacttcttcc gcgatccgcg atgagcaagc gttgagtttt
     2821 gggttgatgc caaccctgca attcttcctg gctgaagaag ctcctttgaa caccgctgct
     2881 gttcggagca cgcgcggaat cttgatcttc tccggcgcac tactcgggtt tttgattgcc
     2941 atcatcatcc ccaacaaaga gaaagaataa atggacgaac gcaaagattt gtggctgatt
     3001 gcacaaaagg tcgcagatat tctctggatc atcatgctcg ctttgttgcc catcacgagc
     3061 atgccagcca tcgcgaggct tctgggttcc gacacggttg cagcaccctc catacttgtg
     3121 gtcttctgcc tgttgctgat atggttcatc ccctatctgc tacgacgcgg caggatggta
     3181 aaagagagca ttcctttgtt ggtatttgtg atcgccgctc tggtgtcaac cctgctggcg
     3241 aattttcaag tgattccacc ctacaaagat ataccatacc tcagcaattc cgttgaagcg
     3301 ttcatgaccc tgttgatcgg ggtggtcttt ttcctggtca cttcatctta tctccgcgat
     3361 gaaaagaaag ccatcttgac gttgcgagtg atcaattgga gcgccatggt catgtttttg
     3421 tggactgcat gccaggtggt cgcctggcac ggttttggtc attaccctgc atggatgttc
     3481 gatctgcagg ggttgatctc ttcccgtgtt ctctaccggc aacgagtgaa cgggctggca
     3541 ttggaaccgt cctggtttgc gcatcaattg aatatggttt acttgccctt ttggctggca
     3601 gcgaccatca agggattttc agcattcaaa aaacgcctgg gttttctcac ggtcgaaaac
     3661 atactgctga tgattggaat cgcttctctc ttcctcacac tctcacgagt gggctggctg
     3721 ggatttttat tgatgttcac ctttttgctg gtcaagctgc attcctacct ggtgagaagg
     3781 gtatcggctg cacttgaaaa aaagcaagct caagttgcgc gaaagcacga aaaacgttta
     3841 aggatcggga tctcaatttt tttcatgatt gtctatgtat tgatcatcgc gattgcgctg
     3901 tatgtttttt caaaaatcga tccgcgcatg gaaagtttgt ttgatttttc tgtgggcaag
     3961 gataatccga tactggcata tgtcagtgaa ctcagctttg gagaacgggt ggtttactgg
     4021 ctggcaggtt ggaacgtttt ttccgagcac ccacttttcg gagtgggatt agggaatgct
     4081 ggcttttatt ttcccaaagc catcacgccg tacggatgga cattgattga ggtacggcac
     4141 ctgacttatc gaacgtccat tttgctcaat gtgaaaagcc tgtgggcgcg gctgctggct
     4201 gaaacagggc tggtgggttt cgccatcttc tgcgcttggt tgtacagcct ggggcgcaag
     4261 tttgtgcaga agacggaaat caagggatcc atccaaggag tttgcgggct ctcaggcgtc
     4321 ttcgtgcttt gcgccctgtt ggcagagggc ttcagcatcg attctttcgc catgccctat
     4381 atgtgggttg ccctgggttt ggcggctgct gacctggctc tgccagcccg tgaaggataa
     4441 cgaattgaat aaaaaagttc tcctcatttt cggaggcatt cttcttttga tcctcgcggc
     4501 aggcatatat ttgactggtc ataagatttt gccgacgcaa aatctggatg tttcctcggc
     4561 acaagaaaac ctgaatgtgt tgatgcaaac agagatcccg gaaaatgacg cctatgctat
     4621 agctgcgaaa tataaaaaca tccatcttga cccgcatgaa ccagcgcacg aaaaaatcac
     4681 ctatgccctc ggggatacgc gccaattcta tattctcgac atggtcacca atgcatatac
     4741 agccatcaat gctaccctgc agtacagcac cgcacatctt tatttctgga ttgaagaaaa
     4801 tgttgatttt gcgcgagaag atgtggcgaa gttatgtgag gagttcgaag ggcagatcta
     4861 tccgctgaac cgggagttct ttggaagtga aaagaccccc ggtattgatg atgatgaaca
     4921 tttgttcatc gtatacacca caaagatgac aggcgctttg ggatattttt catcggcaga
     4981 tacgttgccg caagccatag agccgtattc caacgaagca gaaattttca tgctttctgc
     5041 cagttacgcg catttggacg atgaatcgac ctatggggtg ctggcgcatg aatttcagca
     5101 catgatccac caaaaccttg atgcaaatga aagcacctgg atcaatgagg gcttttctga
     5161 actggcagtc cttttaaacg gatatgatcc gggtgggact gattggttat acgcgcgcaa
     5221 cccggacatt caattgaatt tttggccaag agatgaagtt gaaagcaccc ttccacacta
     5281 tggtgcttca tttatcttca tgacctatct ctacgatcgc tttggggaag aattttccag
     5341 aagactggtg gctgatccgg cgaacgatct ggcgtcgatc gacgaggtgc tgacagacct
     5401 tcagaagcga agcgatggag agcggcttaa cgcagatgac gtttttcaag actggtctat
     5461 cgcaaacctg ctgcaggaca ccgccataga agatgggagg tatggctaca agagcctgcc
     5521 cgagttgtcc agtttccgcg tggatggaaa gatcgattgt acaacggctg tcaagaaccc
     5581 attcaccgta cgtcaatatg gagtggatta cttttcggtg aagtgcgacc atccgctgac
     5641 aattcaattc aatggtgaac ggtctattcc ggtggtgccg gcggatccgc attcagggga
     5701 gtactatttc tggtcgaaca aagctgacca aagtgatatg acgctcacgc agacttttaa
     5761 ttttcgcaat gtggaaggtc ctattattct cgagtatttt gtatggtatg accttgaaac
     5821 agactgggat tatgtttacc tgctggctag cgaaaatggg aaggagtggc ttcaattgaa
     5881 acctgcccgc tgcacggaag agaatatcag cggttctaac ttaggatgcg gctacaatgg
     5941 agccagcagg ggatgggtgg tggagaacgt agacctgaca aagtatgctg ggaagaccgt
     6001 caccttgcag ttcgaatatt taacagatgc ctcgttaaca ggagagggtt ttctgctgga
     6061 tgacgtgcgc atcgaaagca tcggatatga aaccgatttt gaagaagatg agggtggttg
     6121 ggttgctaaa ggttttgtgc ggatgatgaa cagcttgccg caaacctatc gggtggcgat
     6181 catcaataag agtgtagaag aaaccacggt caggcgctat acagttctgg ggaatgaacc
     6241 cttaaccctc gatctcaacg ccaccaccaa tgaagagata tttttggtgg tcagcgggac
     6301 tgcacggtat acagaaatgc ccgcttccta tacgatcgaa ttgctcgatt agcgaaggca
     6361 tcattcctgc gctttaaagg agttgatctc ttcttccagt tcccgtttct tttgggcagc
     6421 ggctttcttt acctcaaggg tgatctgccc ggttttctct tggatctctt tgcgcagttc
     6481 ttcaccgctc ttgggagcgt aaaggagagc tacgatgctg ccaattgccg ctccgagcaa
     6541 agcccctgct acaaaactgg atgatctgct catggttctc ctttcttttc acgtttgctg
     6601 ttgaaccaat taccggatgg aaaccatcca atttaagtat aatgtaaaca tgtatgttta
     6661 ggaaatataa agaacgtggt tttgacgcgt tggcgagctg ttcgctgttg atcatggtat
     6721 ttttatcagc ctactcgctt gaatccacgc attggacaga agatctgaac atggtgacca
     6781 gtctggcatg cattggagtc attctgggta tcgcgctggg cgtcactgtt tttcaaaagc
     6841 gacacatcat gatcttgtcc acgttgtaca gtttgatcct gctctttttg ttcctggtcg
     6901 taattccatc tggcggcaat tcctggagtg agggatggcg gatggtgtac gggcgcactc
     6961 atatggcatt gcgggcattg ctgcaggggc agccggtgcc cgatgccatc cttttcatta
     7021 tcactgcggg catcttgtat tggaccgttt cactatgggc agggtttgcc ctgatcagaa
     7081 aagccaaccc atgggtgccg ctcggtgtgc agggagccag cctggtagcc actcaatttt
     7141 tccaacccgt tctctatcgc agttcattat tgaccggcac ttttttcttc ctgtttctat
     7201 ttttaatagg taggctgaat tttctcatcg cgcatagccg gtggaaagaa cgcaaagcat
     7261 atgaagatca ggaatcgggc accatattcc tgcgaaccac tgccatcctt tcaagcgtac
     7321 tggtgctgtt agcctggggt gttccttttt tgatggatgt ggcgacaccc ggaagcaaac
     7381 agcataaagc gtttatacag actatcgagg aaggagaaga tttcttttcg gacctttttt
     7441 tctccttccg aacgcaaccg atccaaacag agacagtgtt cggggatact tttgatctcg
     7501 gttccgctca accactgggc gaggaggtgt tgtttaccgc ggtcgctccg tccagcgatt
     7561 tttttgatgg caattattac tggaaggcgc gcagttattc cacatatgaa aatggaagat
     7621 ggagttcgat tgatgtggaa ggaaagacca tcgatgcgaa tacccggctt gaacttgcag
     7681 gagcgacgga aaatgagatc ggcacgttca ccatggtggt caacgctgaa ctcacttatt
     7741 tttacctgcc cggcaatact atttcgatca acagagatgt gcgcatcacg gagatgtttg
     7801 caggtgacga ggat
//
LOCUS       DAOSDS010000095         7519 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3592_length_7519_cov_5.271838, whole genome shotgun sequence.
ACCESSION   DAOSDS010000095 DAOSDS010000000
VERSION     DAOSDS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7519
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3592_length_7519_cov_5.271838"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..892)
                     /locus_tag="PLL88_09490"
     CDS             complement(<1..892)
                     /locus_tag="PLL88_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ01837.1"
                     /translation="MEVCSCSKSVCCAGRKPNERRNFMKRSIVPVLALLVVLILTACS
                     AKPAKDNAELIPVTLPVGYIPNVQFAPLYVAIEKGYFAEQGIALTVDYNMETDNVALV
                     GAGNLDFAVVSGEQVLLGRAQGLPVVYVMTWYQRFAVGVAALQESGIREPADLVGKKV
                     GIPGLYGASYIGFRALLQANGLQESDLTLDSIGYTQVETLVNRQEDAVVIYVSNEPIR
                     LRAEGYDVNVIPLYDYVELAANGLITNETAIREKPDLVRGMTAALLKGLVYTSDPHNN
                     AEVFSICEKYVENLASMSATD"
     gene            complement(837..1670)
                     /locus_tag="PLL88_09495"
     CDS             complement(837..1670)
                     /locus_tag="PLL88_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01838.1"
                     /translation="MRAKSALKAFRVFCLMMKKQRTANSNHQPPATWTYPLALVLFLA
                     AWQFLVVIFQFPAFILPSPAQVGERFLTAWTDGSLLRHLLATLSEVLSGLAIGVLGAF
                     LLGYLLSRSKFLERVLSPFLVASQAVPIAAIAPLLIIWFGSGVLSKVLICSLTVFFPM
                     LVNTLIGLHAVPATLRDLMRTLHATRWQTFTRLELPASLPVLLGGMRVSATLSVIGAV
                     VGELAGADKGLGFLINVGRGQYDTALVFVAVLTLVLLALILYGSVLLLEKRLLRWQEA
                     K"
     regulatory      complement(1670..1802)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1962..3302
                     /locus_tag="PLL88_09500"
     CDS             1962..3302
                     /locus_tag="PLL88_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ01839.1"
                     /translation="MLVVISDLHLEECSLGVQRALRVNRNLEPKSYQHIMREIAKDAA
                     VNRAKSVDMVLAGDIFELNRSNIWYEDSIKPYVNLKDIKAGSEHEKKILQLLDLIGKE
                     EKVAGTLEIFRELPSYFAVDFRLHYILGNHDRLVNATPATRRKARKLLGMTASDEPLP
                     HTFTYFSEGKPLAFVRHGQEYDPLNFAVNYKLAPLIPEEIPIEDYEAPPVDDFSAVEI
                     VYKLPYLFVEHYGAEMILQDEKLLNLYHRLVEFQDVRPQSALINYIFHSPGFTRRQIW
                     QYLEPVILHGLNTLRKDPFLKSPTMKGNLLKTFLRLNPFRRNIPFRLVRRFSSSITKR
                     IGRNERNLPAVKEEVLQKNGDSVRCIISGHTHQPQVALMDAEDGVERYYFNSGTWRNV
                     VPVSANQVNFGRIKTFSNVIIYGAEENQRRGEKKSSWSFDFSAGFGQKFYGETG"
     gene            complement(3341..4375)
                     /locus_tag="PLL88_09505"
     CDS             complement(3341..4375)
                     /locus_tag="PLL88_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01840.1"
                     /translation="MFSTYPVLFICGSLNQTRMMHKIAMQLERIESIEACFTPYYADG
                     FIDLLARAGLLRATILGGRHYRDTLQYIKEHRLTLDLRGKRKQYALVVTCSDLIVQKN
                     IRGRRLMLVQEGITEPESFAFQLVKYLRFPRYLANTAATGLSDAYDYFCVASEGYKNL
                     FIKKGVRSEKISVTGIPNFDEMQNYCKNDLPLHDYVLVATSPLRETGQFDNRQAFIKQ
                     CLKIADGRLLIFKLHPMEKVRRVYREIRSLAPHARIYARGNTEHMIANAAVVITQRST
                     CTFTALALQKEVHSYLNLDQLKDLMPLQNNGASARNIALLCSHLLQQPLAHRQKDEQG
                     FKAVLEWIGL"
     gene            complement(4387..5088)
                     /locus_tag="PLL88_09510"
     CDS             complement(4387..5088)
                     /locus_tag="PLL88_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HOJ01841.1"
                     /translation="MQKKITTLFIDLDGTVYDKNNSIMEKMTERIASYMQQFLHIASE
                     DAQKRIDHYYQTYGSTLRGIQEEFSIDTVEYLDYIHEIDLDHYLKPDETLHDILAGID
                     IPKWIFTNATRAHAERVLSKLAIADQFEGILDVWTMDYVPKPHPWVYQHALEMVGDPD
                     PWECIFVDDSARNLKPAHHIGISTIWVGADEIPLHADLSIPHLYDLPIAVEMITEDLI
                     LPEFSTPFASLQMVS"
     gene            5226..5678
                     /locus_tag="PLL88_09515"
     CDS             5226..5678
                     /locus_tag="PLL88_09515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ01842.1"
                     /translation="MESDAPFTIQQASWRDLQALHHLERACFAKDAWPMLDLLGVLTF
                     PGMVHLKADWEGKMVGFIGGETRDHQQTGWIITLGVLEEYRGHGIAKALLEECERKLA
                     TRTIRLCVRVGNEPAIWLYKNAGYIQAEIWPQYYRGGEDALVFEKYLH"
     gene            5699..6562
                     /locus_tag="PLL88_09520"
     CDS             5699..6562
                     /locus_tag="PLL88_09520"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOJ01843.1"
                     /translation="MQATTVVDCRNELGEGPVWHPQEKKLYWLDILEGKIQRLEPVSM
                     EYETFAVGQRISAIGMRAKGGFICAGENGFAFWDRERGAFQPICHPEQGKANARFNDG
                     KVDRQGRFWAGSMTTTDASSTLYRLDKDLVLTPMICNVTISNGIGWSPDDQVMYYNDT
                     RRFTMYAYDFDPASGEISGKREFVRFGGKDGSPDGLTVDAEGYIWCALWGGWRVERYS
                     PQGERVQTIEVPVAQPSSVAFGGEDLSTLYITSAREGLSAENLAEQPQAGNLFACQPG
                     ARGLPEPEFGG"
     gene            6643..6858
                     /locus_tag="PLL88_09525"
     CDS             6643..6858
                     /locus_tag="PLL88_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01844.1"
                     /translation="MNDFCRMFPKVEILFLGAMLVGIVIDVLNGVLALKNSLSQETIL
                     TLSGMGGTALLVSEYYLTRGVARRAYR"
     gene            6865..7485
                     /locus_tag="PLL88_09530"
     CDS             6865..7485
                     /locus_tag="PLL88_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOJ01845.1"
                     /translation="MDNWTLFLKGTGLGISIAAPVGPIGVLCIQRTLKKGFSSGVASG
                     LGAASADLLYGSLAAFGALWLGNHLVQLQTPLRLLGGGVLILLGVRAFFQKEKEATVA
                     GSSPHLAGDYFSTFLLTLTNPITIFSFAAVYGGLGLGSAETSTWQMGLLVLGVFVGSV
                     AWWLTLSSLVALLRGRFKPQFYKVINKIAGVVLVGFGVSLLLALLK"
BASE COUNT         1813 a         1819 c         2271 g         1616 t
ORIGIN      
        1 tatcggtagc ggacatggaa gccaggttct cgacatattt ctcgcagatg ctgaagacct
       61 cggcattgtt gtgcgggtcg ctggtgtaga cgagaccttt gaggagggcg gcggtcatgc
      121 cgcgcaccag gtcaggtttc tctcggatgg cggtctcgtt ggtgatgaga ccgttggcag
      181 ccagctcaac gtaatcatag agagggatga cattcacgtc gtagccctcg gcgcgcaggc
      241 ggataggctc gttgctgacg tagataacca ccgcgtcttc ctggcgattg acgagggtct
      301 cgacctgggt gtagccaatg gaatcgaggg tgaggtcgct ttcttgcaaa ccgttagcct
      361 ggagcaaagc gcgaaagccg atatagctgg cgccatacag accggggatg cccactttct
      421 tgcccaccag gtcggcgggt tcccggatgc cgctttcttg cagggcggcg acgcccaccg
      481 cgaagcgctg ataccacgtc atgacgtaca ctacgggaag tccctgcgcg cgacccaaca
      541 gcacctgttc tccggagacg actgcaaaat ccaggttgcc ggcacctacc agggcgacgt
      601 tgtcggtctc catgttgtaa tcgaccgtca acgcgatgcc ttgctcggca aaatatcctt
      661 tttcgatggc gacgtacaga ggagcgaact gcacattggg gatgtacccc accggcaggg
      721 taaccggaat cagttctgcg ttgtctttcg ccggtttcgc agagcacgcg gtcaggataa
      781 ggacgaccag aagagctaaa actggaacaa tggatctttt catgaaattt ctccgttcat
      841 ttggcttcct gccagcgcag cagacgcttt tcgagcagga gcacacttcc atataaaatt
      901 aatgccagca acaccagggt gagcactgcc acaaagacca gggcggtgtc gtactgaccg
      961 cgcccgacgt taatgagaaa acccagcccc ttatcagccc cagccagttc gcccaccacc
     1021 gcgccgatga ccgaaagggt ggcgctgacg cgcatacccc ccagcagcac cggcagtgaa
     1081 gccggcagtt ccaggcgggt gaaggtctgc cagcgggtgg cgtgcagggt gcgcatgagg
     1141 tcacgcaggg tagccggcac ggcgtgcagc ccaatgaggg tgttgacgag catggggaag
     1201 aagacggtaa gcgagcagat gaggactttg gaaagcacac ccgagccgaa ccagatgatg
     1261 aggaggggcg cgatggcagc gatgggcacc gcctgactgg cgaccagaaa tggggaaagc
     1321 acgcgctcga ggaatttcga acgggagagc aaatagccaa gcagaaaagc acccaaaacg
     1381 ccgatcgcca aaccgctgag cacctcactg agggtggcga gcaggtgacg cagcagactg
     1441 ccatcagtcc aggcagtgag gaaacgttcg cctacctgtg cgggggatgg gaggatgaag
     1501 gcagggaatt ggaagatcac caccagaaac tgccaggcgg caagaaaaag caccagcgcc
     1561 agtggatagg tccaggtggc ggggggttgg tggttgctgt tggcggtgcg ttgttttttc
     1621 atcattaaac aaaaaaccct gaaagctttc agggctgatt tggcgcgcat actcccttct
     1681 tcattccgga ctgtaccgtc gaccccggaa ttccaccgga tctgcgcgca ggcgcgctcg
     1741 tgggttataa ccaccgatcg ggaattgaga gcgtactctc gcaccctgcc ctgaaggact
     1801 ttctgaaaga gagtatactg ctggcgggac aggctgtcaa gaacgggagg gaaacaagag
     1861 gaaaacacaa acaaacgtta ataaaatgat aagctttttt tcatgctgaa tcgaagagag
     1921 tgtgatataa aagcccagga aagtattgaa gagagacgat catgttagtt gttatttctg
     1981 acctgcacct ggaagagtgc tcactgggag tgcagcgcgc tttgcgggtg aaccgcaacc
     2041 tcgaaccgaa atcttaccag catatcatgc gggagattgc aaaagatgcc gcagtaaacc
     2101 gagccaaatc ggtcgatatg gtgctggcag gggatatttt tgagttgaac cgttcgaata
     2161 tctggtatga ggattccatc aaaccttacg tgaacctcaa ggatatcaaa gcgggatcgg
     2221 aacacgagaa gaaaatcttg cagttattgg atctgatcgg gaaagaagag aaggtagcgg
     2281 gaaccctgga aatattcaga gaactgcctt cttattttgc tgtggatttt cgactgcatt
     2341 atattttagg caaccatgac cggttggtga acgccacacc ggctacccgg cgcaaagcgc
     2401 gcaaactgct gggcatgacg gcgagcgacg agccgctgcc gcacaccttc acctatttca
     2461 gcgagggcaa accgctggcg ttcgtgcggc atgggcagga atatgacccg ctaaactttg
     2521 cggtgaacta caagctcgcc ccgctcatcc cggaagagat cccgatagag gattatgaag
     2581 cgccaccggt agatgatttt tcggcagtgg agatcgtgta taagctgccc tacctgttcg
     2641 tggagcatta cggagcggag atgatcttgc aggatgaaaa attgctgaac ctgtatcatc
     2701 gcctggtgga attccaggac gtgcgcccgc aatcagcgct gatcaactac atttttcatt
     2761 cgccggggtt cacgcgcaga cagatctggc agtacctcga gccggttatt ctgcacgggc
     2821 tgaatacgtt gcgcaaggac ccgttcctga aatcgcctac gatgaaagga aacctgttaa
     2881 aaactttcct gcgcctcaac cccttccgca ggaacatccc tttccggctg gtgcggcgtt
     2941 tttcatcttc catcacaaaa cggatcggaa gaaatgaacg taatctaccc gcggtgaagg
     3001 aggaagtgct gcaaaagaac ggcgattcgg tgcgctgcat catctcaggg cacacccacc
     3061 aaccgcaggt ggcactgatg gatgccgaag atggcgtgga acgctattat ttcaactcgg
     3121 gcacctggcg aaatgtggtg ccggtatctg ccaatcaggt gaactttggg cgcatcaaaa
     3181 cattttccaa tgtgatcatc tacggagcgg aagaaaacca gagacgaggt gaaaagaaat
     3241 cttcctggtc attcgatttt tcagccgggt ttggtcaaaa attctacggc gaaacaggat
     3301 aagtttgaaa tacccacgag aagcggaaca gggaattgct tcacaatccg atccattcga
     3361 gaacggcttt aaagccctgt tcgtcctttt gccgatgagc caatggctgt tgcagcaggt
     3421 ggctgcacaa cagggcgata ttgcgggcag atgccccgtt attttgcagg ggcatgagat
     3481 ctttgagctg atccaggttg agatagctgt ggacttcttt ttgcagggct agcgctgtga
     3541 aggtgcaggt ggagcgttgg gtgatgacca ccgcggcatt ggcgatcatg tgttcggtat
     3601 taccgcgggc atagatgcgg gcgtgggggg caagagaacg aatctcacgg tagacgcgac
     3661 ggactttttc cataggatga agtttaaaga tcagcaatct accatcggca atcttcaagc
     3721 attgtttgat aaacgcctgg cgattgtcga actgcccggt ttcgcgcaag ggggaggtgg
     3781 cgaccagaac gtagtcgtgc agaggaaggt cattcttaca gtaattctgc atctcgtcaa
     3841 agttggggat gccggttacg ctgatcttct cgctcctgac acccttttta atgaaaaggt
     3901 tcttatatcc ttcagaagcc acacagaaat aatcgtaggc atctgaaaga ccggtagctg
     3961 cggtgttggc gaggtagcgc gggaagcgca gatatttgac caactggaaa gcaaaactct
     4021 ccggttcggt gataccctcc tgcacaagca tcaaacgcct gccacggatg tttttttgaa
     4081 caatcagatc gctgcaagtg acaaccagag catattgttt gcgcttcccg cgcagatcca
     4141 gagtgaggcg gtgctctttg atgtactgca atgtatcgcg gtagtgtcga ccgccaagaa
     4201 tggttgcccg caacaatccc gctctggcaa gcaggtcaat aaagccgtcc gcgtaatacg
     4261 gtgtgaagca ggcttcaatg ctctcgatcc tttccaactg catggcgatc ttgtgcatca
     4321 ttcttgtctg gttgagtgaa ccacagatga aaaggacggg atatgtggag aacatgtgca
     4381 cctggcttat gaaaccatct gcagcgatgc gaacggcgtg gagaattcag gcaagatcag
     4441 gtcttcggta atcatctcga cagcgatggg caaatcatac aaatgcggaa tgctcagatc
     4501 ggcgtgcaga gggatctcat cggcgcccac ccaaatggtc gaaatgccaa tgtgatgagc
     4561 aggcttcagg ttgcgagcgg aatcatcgac gaaaatgcat tcccatgggt ccgggtcacc
     4621 aaccatttcc agagcgtgct ggtataccca tggatggggt ttgggaacgt aatccatggt
     4681 ccaaacatcc aggatccctt cgaactggtc ggcaatggcg agctttgaca gcacccgctc
     4741 tgcatgagcg cgggtggcat tggtgaaaat ccattttgga atgtcaattc cggcgaggat
     4801 atcatgcagc gtttcatccg gcttcaaata atggtccaga tcaatctcgt gaatgtaatc
     4861 caaatattct acggtgtcga tcgaaaattc ttcctgaatt ccacgcagag tggatccgta
     4921 ggtctgataa taatgatcga tacgcttttg tgcgtcttca gaagcaatat gcagaaactg
     4981 ttgcatgtag ctggcaatcc gttctgtcat tttttccatt atgctgttat ttttgtcgta
     5041 aacagtccca tcaaggtcaa taaagagcgt tgtaatcttt ttttgcatgt tgagagtata
     5101 ccgagcgtat gtcaagaaaa agaaaagaac aggttaacaa attgttaggt tctctggcgg
     5161 acagagagaa cggttgcgat caaaatccct agcgcaaggg aagtcacttt cggtacaatt
     5221 aacgcatgga aagcgatgcg cctttcacca tccaacaagc aagctggcga gacctgcaag
     5281 cgctgcacca tctggagcgg gcatgcttcg caaaagatgc ctggccgatg ctggacctgc
     5341 tgggagtgct gacatttccg gggatggtac acttgaaagc cgactgggaa gggaaaatgg
     5401 tgggtttcat tggaggagag acgcgcgacc accagcaaac cggatggatc atcacgctgg
     5461 gcgtgctgga ggaataccgc ggacatggaa tcgcgaaagc gctgctggaa gaatgcgaac
     5521 gcaagcttgc cacgcgcacc atacgcttgt gcgtgcgtgt cggcaacgag cccgccatct
     5581 ggctgtataa gaacgccgga tatattcagg cggagatctg gccgcaatat taccggggcg
     5641 gggaagacgc gctggttttt gagaagtacc tgcactgatc aaaaaaagga agggaagtat
     5701 gcaagcaaca acggtcgtag attgtcgcaa cgaactggga gaaggacctg tgtggcaccc
     5761 gcaggaaaaa aagttgtact ggctggatat tctggagggc aaaattcagc gcctggaacc
     5821 ggtgagcatg gaatatgaaa cgttcgcggt tgggcaaagg atcagcgcta tcgggatgag
     5881 ggcaaagggc ggattcatct gtgccggaga gaacgggttc gccttctggg acagggagcg
     5941 gggagcgttc cagccgattt gtcaccccga gcagggaaag gcgaacgcac gcttcaacga
     6001 cggcaaagtg gaccggcagg gacgtttctg ggcgggcagc atgaccacca cggatgccag
     6061 cagcacactg taccgattgg ataaagacct ggtgctgacc cccatgatct gcaacgtgac
     6121 catctccaac gggatcgggt ggagcccgga tgaccaggtg atgtattaca acgacacacg
     6181 gcgtttcacg atgtacgctt acgattttga cccggcgagt ggggagatca gcgggaaacg
     6241 ggaattcgtg cgttttggtg gaaaagacgg ttcaccggac gggctgacgg tggacgccga
     6301 agggtatatc tggtgcgcat tgtggggcgg gtggcgggtg gagcgctatt ctccgcaagg
     6361 ggaacgggtg cagaccatcg aagtgccggt ggcgcagccc tcatccgtcg cgttcggggg
     6421 tgaggatttg agcacactct acatcacctc agccagggaa ggattgagcg cggagaacct
     6481 ggcagagcag ccccaagcag gcaacctgtt cgcctgccaa ccgggagcgc gggggttgcc
     6541 agaaccggag tttggcgggt aaaagccgag atcatgataa ttcccgatag atccctgcag
     6601 gcggggcaga gtatgcttag gcgatccgca agtggaggag ccatgaacga tttctgcagg
     6661 atgttcccaa aagtcgaaat ccttttcttg ggcgcgatgt tggtgggaat cgtgattgac
     6721 gtccttaacg gcgtgttggc attgaagaat tccctttcac aggagaccat tctgactcta
     6781 tcgggcatgg gaggaacggc gctgctggtt tcggaatatt atctcacgcg cggtgtggcg
     6841 cgccgggcgt accgataggg gcggatggat aactggacac ttttcctcaa agggacgggg
     6901 ttgggcattt ccattgccgc gccggtgggg ccgattgggg tgctgtgcat ccagcgcact
     6961 ctgaagaagg gcttctcttc gggcgtcgca tccggtttgg gtgcggcaag cgctgatctg
     7021 ttgtacgggt cgctggcggc tttcggcgcg ctgtggctag gaaatcattt ggtgcagttg
     7081 caaaccccct tgcgcttgct ggggggcgga gtgctcattt tgctgggggt gcgagctttc
     7141 ttccaaaaag aaaaggaagc gactgtggca ggcagctcgc cgcatttggc aggggattat
     7201 ttttccacgt tcctactgac gctgaccaac ccgatcacga tcttctcttt cgcggcggta
     7261 tatggcgggt tggggctggg cagcgcagag accagcacct ggcagatggg gctgctggtg
     7321 ctgggggtgt tcgtcggatc ggttgcctgg tggctgacgc tgagcagcct ggttgcgctg
     7381 ttgcgcggac gcttcaagcc tcagttctat aaggtcatca acaagatcgc gggggtagtg
     7441 ctggtggggt tcggggtcag tttgttgctg gcattgctga aatagaaggg gatcaagata
     7501 cgggtttctg atgctgggt
//
LOCUS       DAOSDS010000096         7433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3663_length_7433_cov_4.980076, whole genome shotgun sequence.
ACCESSION   DAOSDS010000096 DAOSDS010000000
VERSION     DAOSDS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7433
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3663_length_7433_cov_4.980076"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="PLL88_09535"
     CDS             <1..869
                     /locus_tag="PLL88_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01846.1"
                     /translation="NRENIIAWEIFSEVNLATGSTESTGTAFVESAAARIRAVDSRQR
                     PTTASLAGFDEWPGFLRNDALDFLNIHPYPETAQLDTFIIQKTRQYLTAYHKPVLIGE
                     SGLSAATPDSEAGQITIADHASIGISHAIWAGVVSGAMNGRALWWEDGVAIYFPELSF
                     PFMAKNSQAELAASAFVQGMDFAGFAPLPTQSSAEITGAALGNESMVIGWFRDAACEP
                     PDWNLRPIPAGQKVTLTRPGLAQDWQVDFYDPQTGTTILSSAFVAPQGETITLTLPAF
                     TDDIAFKLYPRE"
     gene            1160..1885
                     /locus_tag="PLL88_09540"
     CDS             1160..1885
                     /locus_tag="PLL88_09540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOJ01847.1"
                     /translation="MTREIVNFPAALAHQMRHVLRLKEGAEVEILDDQGNALRVRLEV
                     LSDSNALGKIVERSQPQTEPTVKVALCISLTQREKFELLLQKNVEVGTARILPFVSRY
                     SLVREKNIESKRRQRWQDILREAAEQSGRIIIPTLGEVSTLPKLITRCVAEYDLCLVA
                     WEDEHSTSLQESLQGYKKNGDRPQSVAIFIGPEGGFAQQEMELFGQAEVKAVSLGKRI
                     LRMETAAILAPALILYELGQMEV"
     gene            complement(1882..3357)
                     /locus_tag="PLL88_09545"
     CDS             complement(1882..3357)
                     /locus_tag="PLL88_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01848.1"
                     /translation="MPDRSALSPTVASALDRFRSLLSSHEFDDLREMLVQALPPSIRI
                     NLLKHDPATFSRQLQERYGWGLSPVPFCPSGFNLDVPSGTIAKTIEHALGEYYIQESA
                     SMLPVELFDLSPQTAPLILDMAASPGGKTTHLVDKTGDRGLVIANDASNGRIVALRVV
                     LAKWGAINQAITCLHGEYFGSQLPETFDRVLLDAPCSMQNLYDTPSHPLRAVSAGEQQ
                     SLSNRQFLLLRSALQTVKTGGQVVYSTCTLSPEEDEQVVERVLQAFGSQVSLDDVSGK
                     LPKPAPALLRDGNRTFSPEIHKAIRLWPHIFGTTGFFSARFTKHDTILSTEDEKPVPS
                     GKKLNQANFLSFKERQAICRWMQDLYGFDLEGILQTQQATLLENPHGILLVPERLFSQ
                     CNALPASSFGLNAFKRLPVGFQISHELTARFGHTFRTGSVILDEKQSQSFLAHQDIPG
                     VFSGTSTGGETLLVKDESGRVLGRAKVLKDRLKNLLPPRLF"
     gene            complement(3358..4173)
                     /locus_tag="PLL88_09550"
     CDS             complement(3358..4173)
                     /locus_tag="PLL88_09550"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01849.1"
                     /translation="MGLGMLPNEEFVQKILESRKYRDLHLPAETIRDLIRQESARYSD
                     TRRMQESVRRKLHQLTAPYLGDPNYEKELDTLRTIYDPIVLESFADRMLAAHTSTRER
                     QPYLQAFYQTLFQAVGTPHSVLDLACGLNPFALPNMGLDRNVRYSAYDIHLPRIRLLN
                     AFFERAAFPQAHAKQRDILIDPPTQKAEVALFFKEAHRLDQHRKGSNRILWDALQVNT
                     LLVSLPCRDIGKTHDLRERMRRLVMENLEGKTWLVDEIAFEDEIVFCIHKGAG"
     gene            complement(4164..4979)
                     /locus_tag="PLL88_09555"
     CDS             complement(4164..4979)
                     /locus_tag="PLL88_09555"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01850.1"
                     /translation="MSKLPGVDEVTRQVLEKKKYQQVDPELVAFIAASETKKRASLKE
                     AVKATTNKLHQVAWAYLPQQPAYHTHLRELNSLPKEMHAAESRAFCTRLMQEHASTRE
                     RLPILSDFYRTLFQDIQPVTSLLDLACGFNPLAVDWMPVERSATYFACDIFQDMATFL
                     QAFFDHFQINGKAGVCNLLTRLPREKVQVAFLLKTLPCLEQAEKDSARNLLTGIQART
                     ILVSFPVRSIGGKEKGMLAHYSNQFEGLLADLGWSFQKFLFASELAYRVEKWD"
     gene            complement(4976..6061)
                     /locus_tag="PLL88_09560"
     CDS             complement(4976..6061)
                     /locus_tag="PLL88_09560"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOJ01851.1"
                     /translation="MDKTFPTSHGVIPLPAFLPDATFGQVRGLDTQDLIASGVQAVMM
                     NSYHLMQKPGTTVVSALGELHAFSGWQGPIFTDSGGFQAYSLIRQNSSFGSFSDDGIL
                     LKDAASGKKTALTPEKSMQIQWKMGADVLFCLDDCTHVDDADNEQVLSVQRTVHWAKR
                     CKQEFLHLLENSKKDADHQRKLFAVVQGGGSHQYREACAKQLLEIGFDGYGYGGWPLD
                     KDGNLVEDIVGYTRELIPAQFPMHALGIGHPYNLMKTFRLGYQLFDCAMPTRDARHGR
                     LYRLRGEINPDQLNWLEYVYAQDERHIRQKQPIDPSCNCPTCQHYSLGYIRHLHQIGE
                     SLYTRLATIHNLYFMARVIRYLRGESA"
     gene            complement(6110..7027)
                     /locus_tag="PLL88_09565"
     CDS             complement(6110..7027)
                     /locus_tag="PLL88_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01852.1"
                     /translation="MQSREEIIDRLQQADTPSIRLFTLTRLKDLPAGHALVKQEQEQV
                     ANQEPALSILKEQHPEGYWVWDRSHYTPKFKATHWSMQLLTELGLDGNHPAMQQGAVY
                     MRKRMKKHLQEQLQNDERGWICFWGNFAKYQMHCGAFEDATVQETIALQVREIERDSI
                     CDWNYNLPCAWGVIRAMWGLAAISNEKRTSQVQHAIQHGLEFLLERYSLVKADYPYQQ
                     KINRCWFSLNFPLFYNTDILFTLRVLDELHALDHPAARQALDWLQSKQTKNGMWRGAS
                     PARSRTWAFTAGKDTVDDWATLHTLSVLH"
     gene            complement(7055..>7433)
                     /locus_tag="PLL88_09570"
     CDS             complement(7055..>7433)
                     /locus_tag="PLL88_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01853.1"
                     /translation="GAVFLLSAILSRNGGLAIPGCIVGGIGGILYYQEMSGAWDTWAY
                     AWTLIPGFVGVGILLSGLIDRSGPKFESSSLILLAISAAGFIIFGGSFGLGWSVGAYW
                     PVFIIAIGVVVLVSAFIKPKKKE"
BASE COUNT         1654 a         1979 c         2066 g         1734 t
ORIGIN      
        1 agaaccgcga gaacattatt gcgtgggaga tcttctcaga ggtgaatctc gctacgggat
       61 cgacagaatc tacgggcacc gccttcgttg agtcagcggc tgcccgcatc cgggcggtcg
      121 actcccgtca gcgcccgaca actgcttctc tggctggctt tgatgaatgg cccggtttct
      181 tgcgcaacga tgccctcgat ttccttaaca ttcatcccta ccctgagacc gctcagctcg
      241 acaccttcat catccagaag acccgccaat atctgaccgc ctatcataaa cctgtgctga
      301 tcggcgagtc tgggttgagt gctgccactc ccgatagtga agcgggtcaa ataaccattg
      361 cagaccatgc ctctatcggc atcagccatg ccatctgggc tggggtggta tcgggtgcca
      421 tgaatggtcg tgccctctgg tgggaagatg gcgttgccat ttacttccca gaactgagtt
      481 tccccttcat ggcgaagaat tcacaagccg aactggccgc ctccgccttt gtgcagggga
      541 tggattttgc cggctttgca cctttgccga cccaatcttc ggcagagata accggagcag
      601 cattagggaa tgaaagcatg gtgatcggtt ggttccggga tgccgcctgc gaaccccccg
      661 attggaacct gcgccccata cccgccgggc aaaaggtgac ccttacccgc cccggcttgg
      721 ctcaagattg gcaggtggac ttctatgatc cccagaccgg tacgacgatc ctctcttccg
      781 ccttcgtagc ccctcagggg gagacgatca ccctgaccct gcccgccttc acagacgata
      841 ttgccttcaa attgtaccct cgtgagtgac ccatgaggca aagccgactg acgccatacg
      901 cgaagcgaag tagctgaacc ggcagataca gtgcctacca cttatttgcc atctaaatgg
      961 aattattgtt tagtatttat atgaatattg aaagtagcta tgtcatcttt tggttttgca
     1021 cctatttaag aaaataaacg aaatgttttg taaccagaag attgctgatt tccccctata
     1081 atgtatattt aagtcagtgc gtttattctg tgtgaattaa aggaattccg tgcatcgctt
     1141 ctttttgcct gtggaataca tgacccgaga aattgtgaac ttcccggctg cgcttgctca
     1201 ccagatgcgg catgtgctgc gtttgaaaga aggcgcggag gtagagatac tggatgatca
     1261 ggggaatgcg ctgcgggtgc gcctggaagt gctctcggac tccaatgcgc ttggaaagat
     1321 cgtggaacgc agccaacccc aaacagaacc aacggtcaag gttgcattgt gtatctcgct
     1381 cacccagcgt gaaaaatttg aactgctgct gcaaaaaaat gtcgaggtgg gcactgcgcg
     1441 catcctgcct tttgtatcac gctactctct ggtgcgcgaa aagaacatcg aaagcaaacg
     1501 ccgtcagcgc tggcaagaca tcctgcgcga agcggcggaa cagagcgggc gcatcataat
     1561 cccaaccctg ggtgaggtat caaccctgcc caaattgatc acccgttgcg tagcagaata
     1621 tgatctttgc ctggtagcct gggaagacga acactccacc agtttgcagg agagccttca
     1681 aggatataaa aaaaacggcg atcgtcccca atctgtggcg attttcatcg gaccggaggg
     1741 cggttttgcc caacaggaaa tggaattgtt cggacaggcg gaggtaaagg ctgtttcgct
     1801 cggcaaacgc atcctgcgca tggaaaccgc cgccattttg gctccggctc taatcctgta
     1861 tgaattgggg cagatggaag tttagaacaa acgaggggga agtaagtttt tcaaacgatc
     1921 tttgagcact ttggcgcgcc ccagcacccg cccgctttca tctttgacca gcagggtttc
     1981 tccgccggtt gatgtgccgg aaaagacacc gggaatgtcc tggtgcgcca gaaagctctg
     2041 gctctgtttc tcatccaaaa taaccgatcc ggttctaaag gtgtgcccaa aacgggcagt
     2101 cagctcatgg ctgatctgga acccaaccgg gagacgtttg aaagcgttca aaccaaaact
     2161 tgatgccggc agcgcgttgc attggctgaa caaacgctcc ggaaccagca ggatgccatg
     2221 cgggttttcc aggagggttg cctgctgagt ttgcagaata ccctccagat caaaaccata
     2281 gagatcctgc atccaacggc agattgcctg tcgttcttta aacgagagga aattcgcttg
     2341 gttgagcttt ttgcctgaag gaacgggttt ctcatcctca gtagacaaga tcgtatcgtg
     2401 cttggtgaag cgcgcgctga agaagccggt ggtgccaaag atgtgtggcc acaaacgaat
     2461 agccttgtga atttcggggg aaaacgtgcg gtttccatcc cgcagcagag caggggctgg
     2521 ctttggcagt ttgccagaaa cgtcatccag gctgacctgg ctgccgaatg cttgcaatac
     2581 gcgctcgaca acctgctcgt cttcctcggg agaaagcgtg caggtggagt acactacctg
     2641 cccaccagtt ttcaccgttt gcagcgcgct gcgcagcagc aagaactggc gattgctcaa
     2701 actttgctgt tctccggcgc tcacagcgcg cagcggatgc gagggggtgt cgtacaggtt
     2761 ctgcatgctg cagggtgcgt ccagcagcac cctatcgaaa gtttctggaa gctggctgcc
     2821 gaaatattcc ccgtgcaggc aggtgatagc ctggtttatc gctccccatt tggcgagcac
     2881 cacgcgcagg gcaactatgc gcccgttgct ggcgtcgttg gctatgacca atccgcggtc
     2941 gccggtttta tccaccaggt gggtggtctt gccgcccggg gaagccgcca tatccaggat
     3001 cagcggggca gtttgtggcg agaggtcaaa cagttccact ggcagcatcg aagcgctttc
     3061 ctggatgtag tattccccca gcgcatgttc gatggtcttc gcgattgttc ccgaaggaac
     3121 gtcgaggttg aatccgctgg ggcaaaatgg cacaggcgag agaccccacc cgtaccgttc
     3181 ctgcaattgc cgggaaaaag ttgcgggatc gtgtttgagc aaattgatgc ggatggaggg
     3241 aggcaatgcc tgtaccagca tttcccgcag atcgtcgaat tcatgggaac tcaaaaggga
     3301 gcggaaacgg tccaacgcag aggcaacggt aggagaaaga gcgcttcggt caggcattca
     3361 tcctgctcct ttatgaatac aaaagactat ctcatcctca aaggcgattt cgtcaaccaa
     3421 ccaggtcttt ccctccagat tctccatgac aagcctgcgc atccgttcgc gcagatcgtg
     3481 cgtttttccg atgtccctgc agggcagtga aaccagcagg gtgttgacct gcaatgcgtc
     3541 ccacaggatg cggttgctgc ccttccggtg ctggtccaga cggtgagctt ctttgaagaa
     3601 aagagccact tccgcttttt gagtgggtgg gtcgatcaga atgtcgcgct gtttggcatg
     3661 cgcctgcgga aaggctgccc tttcgaagaa agcgttgagc agacggatgc ggggcagatg
     3721 gatatcatag gcggaatagc gcacgttcct atccaaaccc atgtttggca gggcaaaggg
     3781 attcaacccg caagccaggt cgagaacact gtgcggagtg ccaacggctt gaaacagcgt
     3841 ctggtagaag gcttgcaggt agggttgccg ttcacgggta gaagtgtggg cagccagcat
     3901 acggtcagca aagctctcca agacgatggg atcatagatc gtgcgcaagg tatccagctc
     3961 tttttcgtaa ttgggatcac ccagatacgg ggcggtcagt tggtgcagtt ttcggcgcac
     4021 gctttcctgc attcttcttg tgtcggaata gcgcgcgctc tcctgccgga tgaggtctcg
     4081 aatggtctct gctggtaagt gcagatcgcg gtacttgcgc gactccagga tcttttgtac
     4141 gaattcttcg ttgggcagca tgcctagtcc catttttcaa cccggtaagc cagttcgctg
     4201 gcgaaaagga acttttggaa gctccaacct aagtcagcca gcagcccttc gaactggttg
     4261 gaatagtgtg ccagcatgcc tttttctttt ccgccgatgc tgcgcacggg aaaactcaca
     4321 aggatagttc gcgcctgaat gccggtcaga aggttgcggg cgctgtcttt ttcggcttgt
     4381 tccagacagg gcagcgtttt caagagaaat gcgacttgca ctttttcccg cggaagacgg
     4441 gtcagcaggt tgcacacacc ggcttttccg ttgatctgga agtgatcgaa gaacgcttgc
     4501 agaaaggttg ccatatcttg aaagatatca caggcaaagt aagttgccga tctttcaaca
     4561 ggcatccaat ccactgccag cggattgaaa ccgcaagcca ggtcgaggag cgaggttacg
     4621 ggctgaatgt cctgaaagag ggttcggtag aagtcagaaa ggatgggcag gcgttccctg
     4681 gtggacgcat gctcttgcat cagccgtgtg cagaatgccc ggctttctgc ggcatgcatc
     4741 tcttttggca gcgagttcag ctcacgcaga tgggtgtggt aagcgggttg ttgcggcaga
     4801 taagcccatg ccacctggtg cagtttgttg gtggtagctt ttaccgcttc tttgagcgag
     4861 gcgcgttttt ttgtttcgga agcggcgatg aacgctacca gctcaggatc gacttgctgg
     4921 tacttctttt tctccagcac ctggcgggtc acctcatcca cgcctggaag tttgctcatg
     4981 cactttctcc acgcaggtaa cggatgacgc gtgccataaa atacaggttg tggatggttg
     5041 ccaggcgggt gtaaagtgat tcaccaatct ggtgcagatg gcggatgtaa cccaaactat
     5101 agtgctggca ggtggggcag ttgcaggatg ggtcaatggg ttgcttctgg cggatgtgtc
     5161 gttcgtcttg cgcatagaca tattccagcc agttcagctg gtcagggttg atctccccgc
     5221 gcaaccggta caagcgtcca tggcgtgcat cgcgggtcgg catggcgcaa tcgaaaagtt
     5281 gatatcccaa tcgaaaggtc ttcattaggt tataggggtg cccgatgccc aatgcgtgca
     5341 tggggaattg ggcggggatc agttctcgcg tataccccac gatatcttca accaggttgc
     5401 cgtctttatc cagcggccaa ccgccgtatc catagccatc aaaaccgatc tccagcaact
     5461 gttttgcgca ggcttcacgg tactgatggg agccgccgcc ctgcaccacg gcaaataatt
     5521 tgcgctggtg atctgcatct tttttggaat tttccaacaa atgcaggaat tcctgtttgc
     5581 agcgttttgc ccaatgcacg gtgcgctgca ctgaaaggac ctgctcgtta tccgcatcat
     5641 ccacgtgggt gcagtcatcg aggcagaaaa gcacgtctgc gcccatcttc cattggatct
     5701 gcatgctttt ctccggtgta agggcggttt tcttcccgct ggcggcatcc ttgagcaaaa
     5761 tcccgtcgtc cgaaaaactt ccaaaactgc tgttctggcg gatgagcgaa taggcttgaa
     5821 agccaccgga atccgtgaaa ataggtccct gccagccgct gaaagcgtgc agttccccca
     5881 gggcgctgac caccgtggtg ccgggttttt gcatgaggtg gtaggagttc atcatgaccg
     5941 cctgcactcc gctggcaatc aggtcctgtg tatccagacc gcgcacctgc ccgaaggttg
     6001 cgtcgggcag aaaagcgggc agagggatca ccccgtgaga tgtgggaaat gtcttatcca
     6061 tatcttgata agacagattg taacattgag aaagaaaaag ccggggagtt taatgcagca
     6121 cgctcagggt gtgcagggtt gcccagtcat ccactgtgtc cttcccagcc gtgaatgccc
     6181 aggtacggct gcgggcgggg ctggcacctc gccacatgcc gtttttcgtt tgtttgctct
     6241 gcagccaatc cagcgcctgg cgggcagcgg gatggtccag cgcatgcagt tcatccaaca
     6301 cccgcagggt gaacaggatg tcggtgttat agaaaagcgg gaagttcagc gagaaccaac
     6361 aacgattgat cttttgctgg tagggataat ccgctttgac cagagaatag cgttccagca
     6421 ggaactccaa cccatgctgg atggcgtgct gtacctggga tgtgcgtttt tcgttggaga
     6481 tggctgccag cccccacatg gcgcggatca ctccccaggc gcatggcaga ttgtagttcc
     6541 aatcgcagat ggaatcccgt tcgatttcac gcacctgcaa ggcaatggtc tcctgaacgg
     6601 tggcatcttc gaaagccccg cagtgcatct ggtatttggc gaagtttccc cagaaacaga
     6661 tccaaccccg ctcgtcattt tgaagctgtt cttgcaagtg ttttttcatg cgcttgcgca
     6721 tgtacacggc accctgctgc atggcggggt ggttgccatc aagacccagc tcggtcaata
     6781 gctgcatgct ccagtgcgtg gctttgaatt ttggggtgta atgactgcga tcccacaccc
     6841 agtatccttc gggatgttgt tccttcagaa tggaaagcgc cggttcttgg tttgctacct
     6901 gctcctgttc ctgtttgacc aaagcatgcc ccgctgggag atccttcaag cgggttaaag
     6961 tgaacaggcg gatcgagggt gtatccgcct gctgcaaacg atcgatgatt tcttcacgag
     7021 actgcatagt atttccctct cctcgttccc agatctactc ttttttcttg ggtttgatga
     7081 acgcactgac cagcacgacc acgccgatcg cgatgatgaa gaccggccag taagccccaa
     7141 ccgaccagcc caatccaaag ctcccaccaa agatgatgaa tccggcagcg ctgatggcta
     7201 acaggatcaa actgctgctt tcaaacttcg gaccgctgcg gtcgatcaag ccgctcagca
     7261 aaatacccac tcccacgaaa ccggggatca gagtccaggc atacgcccag gtatcccagg
     7321 caccgctcat ctcctgataa tacagaatgc cgccgatgcc accaacgata caaccgggga
     7381 ttgccagccc gccgtttctg gataggatgg ctgacagaag gaacactgcc cct
//
LOCUS       DAOSDS010000097         7137 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3836_length_7137_cov_3.768286, whole genome shotgun sequence.
ACCESSION   DAOSDS010000097 DAOSDS010000000
VERSION     DAOSDS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7137
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3836_length_7137_cov_3.768286"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLL88_09575"
     CDS             <1..382
                     /locus_tag="PLL88_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01854.1"
                     /translation="LLTTHYMAEAEELCHRIAIVNHGRIVVMDTPAALKKHISGDSVI
                     EADVQSAKAQFLLDTLHGLNDRTVVELRSSTEPEKLCIHTGEPEKVLELLSPYLNPEY
                     ILGMEVRNQTLEDVYVTIIQGEEK"
     gene            379..1170
                     /locus_tag="PLL88_09580"
     CDS             379..1170
                     /locus_tag="PLL88_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01855.1"
                     /translation="MRRVRWGLVKQQMAVQLLVRTLNFSSLSLFILQPAVFSAVGMLL
                     SRAAGNAVPDLVYSVIGGGIMGMWSGIVFTSTYDITSDRRNGTLELITGSPTSLASIE
                     AIRTFTNVLCGFFALLVAFAAAMGIFGYSLEGANLVGAGISLLLLLFAMWCSGVFLAN
                     FLAWSRISGTFVEYLEMPMAILCGFMFPMRVLPGWLQTVASVFPIRWALEAMNESLLG
                     MRDLSYLGKHWLIAGGLCLLLMLVTNWLQNKVQDKIRVNGELSSI"
     gene            1167..1967
                     /locus_tag="PLL88_09585"
     CDS             1167..1967
                     /locus_tag="PLL88_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01856.1"
                     /translation="MIKALVRRIRLYIAQAWLSYHGRFSITHPFGYITSKLGFPFFLM
                     IFFIFMGKFVGFSDPLYIVIGNLLLIPANNGMAGITLAIGDERGWGTLSYVLGSPAPR
                     APVFIGRAIFYIVDGFFTALLGFVIAAGIFHMDLSGVGLWLLVLCTFLLAVTASGIGF
                     LFGSISLISRDGWTVLTTFIQALYILVGVNFPVDALPGVLQKVAYGLPLTRGVMAARL
                     VLDGAGWSAIRSLVVGETLIGALYSAAGYVIFRIIEKRSTISGTLDAL"
     gene            complement(2061..2564)
                     /locus_tag="PLL88_09590"
     CDS             complement(2061..2564)
                     /locus_tag="PLL88_09590"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ01857.1"
                     /translation="MHEMTKANLETAFAGESQAHMKYLIFADQAEKDGYPNVARVFRA
                     NAYAEQVHATGHFKVLKKLADTSTNLQAGVDGETYEVEEMYPSYHAVAELQEEPGASR
                     STRWAMEAEKVHAVMYQDAKTAVDGRQDIQEEQIYVCPVCGWTGVGETPDNCPICNAK
                     KELFKLF"
     gene            complement(2670..3389)
                     /locus_tag="PLL88_09595"
     CDS             complement(2670..3389)
                     /locus_tag="PLL88_09595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOJ01858.1"
                     /translation="MKINLHHDDCLNILSKMPHRSIDLILTDPPYNLGIFMRSRQTNL
                     RLMRKNFFGSAGWDDLNVVDWKLRLNSFFREASRVIKIGGAMIIFMAIIKVESIIAIA
                     EQHGFYYKTTGIWHKLNPMPRNMNLHFVNSTEAWIYFTYKEKTGKFNNHGKIIHDFIE
                     TSITNSNEKKHGTHPTQKPICLMEYFIKILSDESDWVLDPFMGSGSVGVAAKRLKRNF
                     IGIEISENYFQISKKRISNQP"
     gene            3512..3880
                     /locus_tag="PLL88_09600"
     CDS             3512..3880
                     /locus_tag="PLL88_09600"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ01859.1"
                     /translation="MDIKILQREVQLLHERVCSAMSDATRIMILYLLSEKSMYVNEIA
                     DALEQPQSTISRHLKILRERGLVATERQGTAILYSLPDRRIIEALDLMRSILNDQVMA
                     AALITHPKQINERSQKEKKK"
     gene            3877..6327
                     /locus_tag="PLL88_09605"
     CDS             3877..6327
                     /locus_tag="PLL88_09605"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF010037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HOJ01860.1"
                     /translation="MKVLIVGGVAGGASTATRLRRMNEEAEIIIFERGEYISFANCGL
                     PYHIGEVISERDKLLVVTPEKLKQTSNIEARVRHEVVSIDRKGKTIKVHDLISGREYT
                     ENYDKLVLSPGASPIIPPIEGVNLPGVYTLRSMTDMDRIRDSILAHKPTRAVVAGGGF
                     IGLEIAENLQHRGIEVTIIEMLNQVMAPIDPEMAAMVHQHLDFKHVRLALGDGIQNIS
                     QNGTLKVTLQSGKTVDTDLVILSVGVRPESTLAKEAGLELGMKGAIKVNSHMLTNDPN
                     IYAIGDAAAVPHIVSGKEVNLPLAGPASKQARIVADHICGRKVEFSGVQGTSIVKVFD
                     LTVASTGLNSNQLKELGLPFKNALIHASNHAGYYPAASPLAIKLLFSPEGKLYGAQIV
                     GLEGVDKRIDVIATAIRGGMSVYDLEKLELAYAPPYGSSRDPINIVGFLAGNMLRGDL
                     KTMDWDQVDALDREEYTILDVRNPQELAIGTIKDYVNIPLPELRTRIKELPRSKKVMV
                     YCQVGQRGYFASRILSQHGLDATNLNGGFKTYSHAVCQQSNFDTFEHITITSREEIHE
                     VPPFDVGDAAQFMVDACGLQCPGPILKLYNKSKEVQPGDVITVRATDYGFTSDVKAWA
                     ERTGNKLLSVDSADGVITARIQKGAEKKAEGTVGAMTQDKTMVIFSGDLDKAIAAFII
                     ANGAASMGHKVTMFFTFWGLNILRKSKAPKVHKNFIERMFGMMMPRGTEKLKISKMNM
                     GGAGTAMIKGIMKKKNVSPLAELMRSAQENGVIIQACQMSMDLMGIKKEELIDGIDVV
                     GVATFVHASDDSNATLFI"
     gene            complement(6866..7024)
                     /locus_tag="PLL88_09610"
     CDS             complement(6866..7024)
                     /locus_tag="PLL88_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01861.1"
                     /translation="MRKHHGFLRFLFVLILLAAGGAIWYAFLSEGQQKFIKNFIKQVP
                     ELPGRYSL"
BASE COUNT         1718 a         1722 c         1900 g         1797 t
ORIGIN      
        1 cctgttgacg actcactata tggcagaggc ggaggagttg tgtcaccgca tcgcgattgt
       61 caatcatgga cgcatcgtgg tgatggatac cccggcggca ctgaaaaaac acatcagtgg
      121 cgattcggtg attgaagcgg atgtgcaatc ggcaaaggcg caatttttgc tggatacgtt
      181 gcacggcttg aatgacagaa cggtggtcga gctgcgaagc tctaccgaac cggaaaaatt
      241 atgcatccac accggggaac cggaaaaggt gctggaatta ctttctcctt acttgaaccc
      301 ggaatacatc ttgggaatgg aagtgcgcaa tcaaacactg gaagacgtgt acgtcaccat
      361 cattcaaggg gaggagaaat gaggcgcgtg cgctggggcc tggtcaagca gcagatggca
      421 gtacaactgc tggtgcgcac gctcaatttc tcttctcttt ccctttttat cttacagccg
      481 gctgttttca gcgcggtagg catgctgctt tcgcgcgcgg cggggaatgc tgtgcccgac
      541 ctggtgtaca gtgtgatcgg tggcggcatc atgggtatgt ggagcggcat cgtcttcacc
      601 tccacctatg acatcaccag tgaccggcgc aacggaacgc tggaattgat caccggcagc
      661 ccgacgtccc ttgctagcat cgaagccatc cgcaccttca ccaatgtgct gtgcggattc
      721 tttgcgttgc tggtggcttt cgcagctgct atgggtatct ttggatattc cctggagggt
      781 gcgaacctgg tgggagcagg catctctttg ctgctgcttt tgttcgccat gtggtgcagc
      841 ggcgtgttcc tggcgaattt tttggcttgg tcgcgcattt caggcacttt cgtggaatac
      901 ctggaaatgc cgatggcaat cctctgcggg ttcatgttcc cgatgcgtgt gctgccaggg
      961 tggctacaga cggtggcgag cgtctttccc atccgctggg cgctcgaagc gatgaacgag
     1021 agcttgctgg gtatgcgcga ccttagctat ttgggaaaac actggctcat agcaggtggg
     1081 ctgtgcttgc tgctgatgct ggtcacgaat tggctgcaga acaaagtgca ggataagatc
     1141 cgcgtgaacg gagaattgag ctcgatatga tcaaggcgct cgttcgaagg atacgattgt
     1201 acattgccca ggcgtggctt tcttatcacg gcaggttttc tatcacccat cccttcgggt
     1261 acatcacatc caaactgggt tttccgttct ttctgatgat cttcttcatt tttatgggca
     1321 agtttgtggg tttcagcgat ccgctgtata tcgtgatcgg caacctgctg ctcatccctg
     1381 ccaacaacgg catggcgggt atcacactcg ccatcgggga tgaacggggg tggggtacgc
     1441 tttcgtatgt gctgggatcc cccgcaccgc gtgcgccggt ttttatcggg cgggcgatct
     1501 tttacatcgt ggacggattt tttaccgctc tgctgggctt tgtaatcgca gccggcatct
     1561 tccacatgga tctgagcggg gtggggctgt ggctgctcgt gctctgcaca tttctactgg
     1621 cagtcacagc cagcggcatc ggatttctgt ttggcagcat ttcactgatc agccgggatg
     1681 gttggacggt tctgaccact tttatccagg cgctgtatat cctggtaggg gtgaattttc
     1741 cggtggatgc gctgccgggt gtattgcaga aggtagccta tgggctgccg ctgacgcgcg
     1801 gtgtgatggc agcccgcctg gtgttggatg gggcgggatg gagtgcgatc cgatcgctgg
     1861 ttgtaggtga aacgctgatc ggcgcattgt acagcgcagc cggatacgtc attttccgca
     1921 tcatcgaaaa gcgcagcacc atctccggca cgctggatgc tttatgattc cctagttccc
     1981 cttcagcaaa ataatgaaga aagaggatgg ttaaagaaga acaggcaacc actcgttggt
     2041 tgcctgcttt cgtcagcgga ttaaaaaagc ttgaacaatt ccttcttggc gttgcagatg
     2101 gggcagttat cgggggtttc gcccacaccc gtccagccgc acaccgggca gacgtagatc
     2161 tgctcttctt gaatatcttg tctgccatcc acggcggtct tggcatcctg atacatgacg
     2221 gcatgcacct tttcggcttc catcgcccag cgcgtggaac gggatgcgcc gggttcttct
     2281 tgcaactccg ccaccgcatg gtaggagggg tacatttctt ccacttcgta agtttccccg
     2341 tcgacgccag cctgcaagtt cgtggaggtg tcagccagtt ttttcagcac cttgaaatgc
     2401 ccggtagcgt gcacctgttc ggcataggca ttggcgcgga agacgcgcgc cacgttgggg
     2461 taaccatctt tctctgcctg gtcggcgaag atgaggtatt tcatatgcgc ctggctttcg
     2521 ccggcgaagg cggtttcgag attggctttg gtcatttcgt gcatggtatt tttctcctat
     2581 tgtcgatgaa ttgggttgat tttgtagcaa ttataatata aatgaggaat cctcattttg
     2641 cctttgcagg gaactttcga acctggcatt catggttgat tcgaaattct tttcttggaa
     2701 atttggaagt agttttcaga tatttcaatc ccaataaaat ttcgctttag ccgttttgct
     2761 gcaactccta cgctcccgct gcccatgaaa ggatcaagaa cccaatcgga ttcatccgat
     2821 aagatcttta taaagtattc catcaagcaa atgggttttt gcgtaggatg cgtgccatgt
     2881 ttcttttcat ttgaattggt aattgacgtt tcaatgaaat catgaatgat ttttccatgg
     2941 ttgttgaatt tgcctgtttt ttctttgtag gtaaaataaa tccaggcttc tgtggaattg
     3001 acaaaatgga ggttcatatt tcttggcatc ggattcaact tatgccaaat gccagttgtt
     3061 ttatagtaaa acccgtgctg ttcggcaatg gcgattatgg actcgacttt aataatcgcc
     3121 ataaaaataa tcattgcgcc accaatcttt atcactctcg atgcttctcg aaagaaacta
     3181 ttcaacctta atttccaatc cacaacattg aggtcatccc aacctgcaga gccaaagaaa
     3241 tttttcctca tcagccgaag atttgtttgt ctgctccgca tgaaaattcc aagattatat
     3301 ggtggatcgg tcaaaatcag gtcaatggac ctatggggca ttttcgatag gatattcaaa
     3361 caatcgtcgt ggtgtaagtt gattttcatt ttccctgccc cgatttcgtt gaatgatggg
     3421 ctctctcaat attatatttg ctcttctcta tggcacaaat ggagaattgt gttatcctat
     3481 atccgtttat ccgaatgtaa agaaataagt gatggatatc aaaatactgc aaagggaagt
     3541 gcaattgctg catgagcggg tgtgctcggc aatgagcgat gccacgcgca tcatgattct
     3601 ttacctgctt tcggaaaaga gcatgtacgt gaatgagatc gcagacgcgc ttgaacagcc
     3661 ccaatccacc atctcacgcc atctcaagat cttgcgcgag cgcggtctgg tggcgaccga
     3721 acggcagggc acagccatcc tgtattccct gcctgaccgg cgcatcatcg aagcgctgga
     3781 cctgatgcgt tccatcctga acgatcaggt gatggcggca gcccttatca cccatcccaa
     3841 acaaatcaac gaacgatctc aaaaggagaa aaagaaatga aagtacttat tgttggcggt
     3901 gtggcaggtg gtgccagcac ggctacccgc ctgcgccgca tgaacgaaga agcagagatc
     3961 atcatctttg aacgtgggga gtacatctcc ttcgccaact gcggattgcc ttaccacatc
     4021 ggtgaggtga tcagcgaacg cgacaagctg ctggttgtca ctcccgaaaa attgaagcaa
     4081 acttccaaca tcgaagcgcg cgtacggcat gaagtggtca gcatcgaccg caagggcaag
     4141 accatcaaag tgcacgacct gatcagcggt cgcgaatata cagagaatta cgataagctg
     4201 gtgctctccc cgggtgcctc tcccatcatc ccacccatcg aaggggtgaa cctgccgggc
     4261 gtgtacacgt tgcgctcgat gaccgatatg gaccgcatcc gcgattctat cctggcacat
     4321 aaacccacgc gtgcggtggt ggcgggcggc ggtttcatcg gattggagat cgcagagaac
     4381 ctgcaacacc gcggcatcga agtgaccatc atcgagatgc tcaaccaggt gatggcgccc
     4441 atcgatcctg agatggcagc tatggtgcac cagcacctcg atttcaaaca tgtgcggctg
     4501 gctctgggtg atggcatcca aaacatcagt cagaatggga cgctcaaggt gaccctgcag
     4561 agcggcaaga cggtggatac cgatctggtg atcctctcgg tgggggtacg acctgaaagc
     4621 acactggcaa aggaagccgg gctggagctg ggcatgaaag gcgccatcaa ggtcaacagt
     4681 cacatgctca ccaatgaccc gaacatctac gccatcgggg atgctgcggc ggtgccccat
     4741 atcgtcagcg gaaaagaggt gaacctgccg ctggcaggac cagccagcaa acaggcgcgc
     4801 atcgtggcgg accacatttg tgggcgcaaa gtggaattct ccggcgtgca gggaacttcc
     4861 atcgtcaaag tgtttgatct gacggtagcc agcaccgggc tgaactccaa tcaactgaaa
     4921 gaactgggat tgcctttcaa gaatgccctc atccatgctt cgaaccacgc cggttattac
     4981 ccggctgctt cgccgctggc gatcaaactg ctgttctcgc ccgaaggtaa actttatggc
     5041 gcacagatcg tggggttgga aggggtcgac aagcgcatcg atgtgattgc aaccgccata
     5101 cggggcggga tgagcgtgta tgacctggaa aaactggaac tggcatacgc gccgccttac
     5161 ggttcctcgc gcgatcccat caacattgtg ggattcctgg cgggcaacat gctgcgcggc
     5221 gacctgaaaa caatggattg ggaccaagtg gatgcgttgg accgtgagga atataccatc
     5281 ctggacgtgc gcaaccccca ggagttggcg atcggtacca tcaaggacta tgtgaatatc
     5341 cccttaccgg aactgcgcac gcgtatcaaa gaactgccgc gcagcaaaaa ggtgatggtg
     5401 tactgccagg ttggacagcg cggctatttt gcttcgcgca ttttgagcca gcacggactg
     5461 gatgcaacca acctgaacgg cggcttcaag acctattccc atgcggtctg ccagcaatcc
     5521 aacttcgata cctttgaaca tatcaccatc accagccgtg aggagatcca cgaagttcct
     5581 cccttcgacg tgggtgatgc ggcacagttc atggtggatg cctgtggctt gcaatgcccg
     5641 ggacccatcc tgaagctgta taacaaatcc aaagaggtgc agccggggga tgtgatcacc
     5701 gtgcgcgcca cggattacgg cttcaccagc gacgtaaaag cctgggcgga gcgcaccggc
     5761 aacaaactgc tctcggtgga ttctgctgat ggtgtgatca ccgctcgcat ccaaaagggt
     5821 gcggaaaaga aagctgaagg cacggttggt gccatgacgc aggacaagac catggtgatc
     5881 ttttcaggcg acctggataa agccattgca gcgttcatca tcgccaacgg ggcggcatcc
     5941 atggggcaca aggtgaccat gttcttcacc ttttggggat tgaacatact caggaaatcg
     6001 aaggcgccca aggtgcacaa gaatttcatc gagcgcatgt tcggcatgat gatgccgcgc
     6061 ggcaccgaaa agctgaagat ctcgaagatg aacatgggcg gggcgggaac cgccatgatc
     6121 aagggcatca tgaagaaaaa gaacgtcagc ccgttggcgg aactgatgcg gagcgcccag
     6181 gaaaacggcg tgatcatcca ggcatgccag atgtcgatgg atttgatggg catcaagaaa
     6241 gaagagctca tcgatggcat cgatgtggtg ggggtggcga ccttcgtcca tgcgtcggat
     6301 gactcgaacg cgacgttgtt tatctagaat taaagagaaa aaagccgtct cttttagggg
     6361 gcggcttttc tgtacaatat taaatatatt ttctagaaaa taaggcactt tattaaaaaa
     6421 tagaattatt taatagataa taaaaaaagg aaggtatatg aatctaatat ttagtgttat
     6481 tacgaagtaa agcgtagaat accgcaaaga aacttttgca tgaaatcggt ataattttac
     6541 agaaagtgcc ataaacgcaa taatctaaga ttatgacttc aggtaacatg gaataaaaag
     6601 caagggaaaa atactacttt ttctttgtat tggttctaac cttttcagtt tcagttgttt
     6661 ttggatgtgt ttttgcataa ctatctttaa caaactttcc tgttattgaa ctcctattta
     6721 cttttcgggt agaagatttc tttgacattt atccctctct ttcaacaata ctattctatt
     6781 gcagagaatt gtcatttaca agagaataaa aagtgtgaac taaacattga ttttgtaata
     6841 atcaagtcac agagattatt tattcttaca aagaatacct tcccggcagt tctgggactt
     6901 gcttgatgaa attcttgatg aatttctgct gcccttcact taggaatgca taccagatgg
     6961 ctccaccggc tgccagcagg atcaaaacga acaaaaatct caaaaatcca tgatgtttgc
     7021 gcatagatgt tatctctccc tggtcaaaac tcactattcg aatttttcaa ttaaattggc
     7081 agtgatcatg cccgatgcga tggtggaagg cactccgccg ccggggtggg tggaagc
//
LOCUS       DAOSDS010000098         7125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3845_length_7125_cov_4.998444, whole genome shotgun sequence.
ACCESSION   DAOSDS010000098 DAOSDS010000000
VERSION     DAOSDS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3845_length_7125_cov_4.998444"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLL88_09615"
     CDS             <1..408
                     /locus_tag="PLL88_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01862.1"
                     /translation="IHPGVAEKYTFLENAVLIADVNLELLSKSLPSSFRSRSVSAYPP
                     VLEDLAMIVPEEISSTDVETVIRKAGGELLSDVVLFDVFKGAQIGSGKKSLAYNLTFQ
                     SFDKTLTDKDVEKSRERIIKALEEQIHAIVRKK"
     gene            complement(448..1407)
                     /locus_tag="PLL88_09620"
     CDS             complement(448..1407)
                     /locus_tag="PLL88_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ01863.1"
                     /translation="MHYRRLGKAGIKVSEISLGSWITFGDQIQEKAAIDMVRAAYERG
                     VNFFDCADVYANGKAETVLGNAVKGLSRPTLVISSKVFWPTFEGVNGKGLSRKHILES
                     IDLSLKRLQLDYVDLYFCHRYDPDTTIEEVVQTMDMLVRSGKVMYWGTSEWKAANIVQ
                     AIEYANHSGLIGPVMEQPQYNLLRRTNVEEDLEPLTRQYGLGLTTFSPLRNGILTGKY
                     NNGIPEGSRASMENMSWLRDQVTAETIARVREFVKLAGELGVTPAQLSIAWVLRRKEV
                     SSVITGASKMEQLDDNLAAAALVERMDEMILNKIDATFNGSPE"
     gene            complement(1408..2760)
                     /gene="ablA"
                     /locus_tag="PLL88_09625"
     CDS             complement(1408..2760)
                     /gene="ablA"
                     /locus_tag="PLL88_09625"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOJ01864.1"
                     /translation="MKDLGFPSKRAKTYQDIPDEKWNDWHWQLSHRLNTVEDFEKIFP
                     LTGSEKKALQSDRLFRVEITPYYASLINPNDPADPVRRQVVPTAAEIVPFTGMMEDSL
                     AEDMHSPVPGLVHRYPDRVLMLVTTQCASYCRYCTRGRIVGDPSATFSREEFERQIDY
                     LQKTPQVRDVLLSGGDPLVLAPKLLEELLTRLRAIPHIEVIRIGSRVPVFMPMRITDE
                     LTDMLKQFHPLWLNIHVNHPNEITAELAEACDRLSSAGIPLGNQSVLLRGINDCVNIQ
                     RKLVQSLVRIRVRPYYLYQCDLVEGAGHFRTPVAKGIEIIEGLRGHTSGFAVPTYVVD
                     APGGGGKIPVMPNYLISASDHKVVLRNYEGFITTYEEPIDYQPHDAGHCAYCQQKNVE
                     PGQAGVLGLLEGEQMVIKPEGFDHLHTRGGERHRLRDNVDKWKPLGIGDGTQEKKEGK
                     "
     gene            2972..4000
                     /gene="hrcA"
                     /locus_tag="PLL88_09630"
     CDS             2972..4000
                     /gene="hrcA"
                     /locus_tag="PLL88_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOJ01865.1"
                     /translation="MEKLTGRQQKILSLIIHEHIRSAEPVASSRLVEEYNLDLSSATV
                     RNEMAFLTESGFLSQPHTSAGRVPTEEGYRYFVGNLMRNTTLPEDARRTISHQFYQTR
                     NDISNWMHLAASILANQSKAASLVTSPHPEQVRFKHLELIATHNTQILCVLVLEGGEI
                     HQNVFTTDVAVNQEDLSRISNSLSVSFRGKNRKEIQDVVHSYSELERSTVNWILLEME
                     QTENLSAGEVILDGLTNVLSEPEFMESDEARRVLKLIEERTLLQDLISRSGFKDQTNG
                     VQVLIGGEGTWDEFKQMSIILGKYGLPGVATGTLGVFGPIRMSYGHSISIVRFLSSLL
                     SDFVAEQW"
     gene            4022..4642
                     /locus_tag="PLL88_09635"
     CDS             4022..4642
                     /locus_tag="PLL88_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOJ01866.1"
                     /translation="MTEKDKHEEPIQEEKKTRPAEAGEAMKEEKEPQEGYTVEAEKQE
                     EMVTIPLKSMEEQLKEIDELKDQVATYSDGWKRERADFDNYRKRVLRDQEQEKQNLTI
                     EIVKKYLVLYDDLGLALKNAPTEPGTKQWVEGIALILKKMQKILEDEGIEPINVDKVD
                     FDPQFHEAISHEEDPDYKSGQIIEVVKPGYRINNRIIRPALVRVAK"
     gene            4653..6575
                     /gene="dnaK"
                     /locus_tag="PLL88_09640"
     CDS             4653..6575
                     /gene="dnaK"
                     /locus_tag="PLL88_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOJ01867.1"
                     /translation="MGKIIGIDLGTTNSVVAVMSGGEPIVIPTAEGERLCPSVVAINK
                     NGERIVGRTARNQAVVNPENTIFSIKRFMGRKYDDPEVQRTIKRVPYKVTKAPNGDVR
                     VVMGGKEYSPPEISAMILAKLKKDAETYLGETVTQAVITVPAYFNDAQRNATKDAGKI
                     AGLDVLRIINEPTASSLAYGLDKKKNEVIAVYDLGGGTFDISILDVGEGVFQVRSTNG
                     DTFLGGDDFDQLVMDYLIDEFKRDQGIDLRNDRQALQRLKEAAEKAKIELSTVMETEI
                     NLPYITADANGPKHMLIRLSRAKLEQLTNELIEKTIPPVEKALSDASLKPSDINEIVL
                     VGGMSRMPAVREIVRQKFGKESHKGVNPDEVVAIGAAIQAGVLGGDVKDILLLDVTPL
                     TLSVETLGGVATPLIERNTTIPTRKSQIFSTASDSQTQVQIHVTQGERSMAADNKSLG
                     QFILDGIPPAPRGVPQVEVTFDIDANGILNVTAQDKATGRSQKITITASSGLTKEEID
                     RMKKEAESFADQDNKRKEVIEARNIADNTIYTTEKTLREGGDKVPADAKREAEDAINS
                     LRKATEGDDIAEIRKQTESLGQILQKIGASMYQQPGAAGPTGQPGQDASASDDGQGQN
                     KPDDGEDVVDGEFKNV"
     gene            6658..>7125
                     /locus_tag="PLL88_09645"
     CDS             6658..>7125
                     /locus_tag="PLL88_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOJ01868.1"
                     /translation="MAEKRDYYEILGVPKNASAEDIKSAFRKLARQYHPDVNHEPNAE
                     EKFKEINEAFAVLGDPEKRAAYDRFGFAGVSGNGAQWDASTIDPFEIFEQFFGFGGIG
                     GSARRRNSPRRGEDIVTSLVLNFEEAFTGVDKDVAITREEVCQVCKGTGSEPGT"
BASE COUNT         1907 a         1771 c         1762 g         1685 t
ORIGIN      
        1 atccatccag gcgttgcgga gaaatatacg ttcctggaaa acgcggtttt gattgctgat
       61 gtcaatctgg aattgctgtc gaaatctctg ccttcttctt tccgcagcag gtcggtctct
      121 gcctatcctc cggtattgga agatttggca atgatcgtgc ctgaagaaat atcctctaca
      181 gatgtcgaaa cggtcattcg caaggcgggc ggggaactgt taagcgatgt tgtcctgttc
      241 gatgtcttca agggcgcgca gatcggcagt ggaaagaaga gtctggcata caatctcacc
      301 ttccaatctt tcgataaaac cttgaccgat aaagacgttg aaaaatccag agagcggatc
      361 atcaaagcgt tggaagaaca gatccacgcc atcgtgcgca agaaataaga tatctaaaga
      421 gctcgttctg cgagctcttt tctttatcta ttccggcgaa ccgttgaagg tcgcatcgat
      481 cttattcagg atcatctcat ccattctttc aaccagggct gcagcagcca gattatcatc
      541 caactgttcc atcttactgg ctccggtgat cacggaactc acttcttttc gcctcagcac
      601 ccaagcaatg gagagctgag caggggtcac tcccagttcc ccagccagct tcacgaattc
      661 tcgcaccctg gcgatcgtct ctgcggtcac ctgatcgcgc agccagctca tgttttccat
      721 ggatgcccgg ctgccctcag gaataccgtt attgtatttt ccggtgagga tgccattcct
      781 taaggggctg aaagtggtca atcccaaacc atattggcgc gtcaatggtt cgaggtcttc
      841 ttccacattg gttcgtctta acaggttata ttgcggttgt tccataacag gacctatcaa
      901 cccgctatgg ttggcatatt caattgcctg aacgatattt gccgccttcc attccgaagt
      961 tccccagtac atcacttttc cgctgcgcac cagcatatcc atggtttgca ctacttcttc
     1021 gatggtagta tcagggtcat agcggtggca gaagtacaga tctacataat ccaactgcag
     1081 ccgttttaag gacaggtcaa tggattccag aatgtgcttt cgcgaaagac cttttccatt
     1141 cactccttca aaagttggcc agaagacctt gcttgaaatg accagagttg gtctggacag
     1201 acctttaacg gcatttccca gcactgtttc tgcttttccg ttggcataca catcggcaca
     1261 gtcgaagaaa ttgacgcctc gctcataagc tgcacgcacc atatcaatgg ctgctttttc
     1321 ctgaatctgg tcgccaaacg tgatccacga gcccaaggag atctcgctga ctttgatgcc
     1381 tgcttttcct aatcttctgt aatgcattta ttttccttct ttcttttctt gtgttccatc
     1441 accgataccc agcggtttcc atttatccac attgtcgcgc aaacggtgtc gttctccgcc
     1501 gcgcgtatgc agatggtcaa aaccctctgg tttgatcacc atctgttctc cttccaatag
     1561 acccagcacg ccggcttgcc cgggttccac atttttctgt tggcaatatg cacaatgacc
     1621 ggcatcatgc ggttggtaat caatgggttc ctcgtaggtg gtgatgaacc cttcataatt
     1681 gcgcaaaaca actttatggt ccgaagcgct gatcaggtaa ttgggcataa ccggaatctt
     1741 gcccccccct cccggggcat ccaccacgta ggtcggaacc gcaaagcccg aagtatgacc
     1801 gcgcaaccct tcaatgatct caatgccctt agccactggc gttctaaaat gcccggcgcc
     1861 ttccaccaga tcacactggt agaggtaata gggtctgacg cggatgcgca ccaaggattg
     1921 caccagcttg cgctggatgt tcacgcaatc attgataccg cgtaacaata ccgactggtt
     1981 ccccaggggg atgccggcgc tgctcaatcg atcgcaggct tccgccagtt cagccgtgat
     2041 ctcattcgga tgattaacat gaatgttcaa ccacagaggg tgaaactgtt tcagcatatc
     2101 cgttaattcg tccgtgatgc gcatgggcat gaagaccggc acacgggatc cgatgcggat
     2161 cacttcgata tgcggtattg cgcgcagccg cgtgagcagt tcctccaaca atttgggagc
     2221 cagcaccagc gggtcaccac ctgaaagcaa cacatccctc acttgagggg tcttttgtag
     2281 ataatcgatc tgtcgttcaa actcttcccg tgaaaacgtg gcgcttggat cgccaacgat
     2341 tctgccgcgc gtgcagtaac ggcagtatga tgcgcactgg gtcgtcacca acatcaagac
     2401 ccggtcaggg tagcgatgaa ccaagccggg caccggggaa tgcatatctt cagccagcga
     2461 gtcttccatc atgcctgtga atggaacgat ttctgcggcg gtaggaacca cctgccggcg
     2521 tacaggatcg gctggatcgt tcgggttgat caaggaagca tagtaggggg tgatttctac
     2581 tctaaaaaga cggtcagatt gcaatgcttt cttctcactg cctgtgagcg ggaatatctt
     2641 ttcgaaatct tcaaccgtgt tcaaacggtg gctcaactgc cagtgccaat cgttccattt
     2701 ttcatccgga atatcctggt aggttttcgc tcttttcgag ggaaatccca aatctttcat
     2761 tttccaatgc tccttagcgt gctatttgat aacttcattt tattgcatat tgggtgggcg
     2821 tcaacgcaat tcataaccat cgttgtatta ataattcata agaaagtgga cacaaacagc
     2881 ccggcttctt gacgcttaaa aatcagcatg gtatgatggt taacatgttt ttagcactct
     2941 aacaacgaga gtgctaaaaa cgagagaaac aatggaaaaa ttaacaggtc gtcaacaaaa
     3001 aatcctgtct ctgatcattc atgagcacat ccgctcagca gaaccggttg cgtccagccg
     3061 gctggtcgaa gaatacaacc tcgacctcag ttcagccacg gtgcgcaatg aaatggcttt
     3121 tctgaccgaa tcaggcttcc tgagccagcc acacacttca gctggcagag tgccaacaga
     3181 agaaggttat cgctactttg ttgggaattt gatgaggaac acgacactcc cggaggatgc
     3241 gcgcagaacc atcagccacc aattttacca gacgcgcaac gatatctcca attggatgca
     3301 tttggcggct tccattctgg caaaccaatc caaagccgcg tcgttggtca cttccccgca
     3361 ccctgaacag gtgcgtttca aacacctgga attgatcgcg acccacaata cccagatctt
     3421 gtgtgtgctg gtattggaag gcggtgagat ccaccagaac gtattcacaa ccgatgtggc
     3481 tgttaaccag gaagatcttt cgcgtatctc aaattcgttg agcgtttcat tccgtgggaa
     3541 gaatcgcaaa gaaattcagg atgtcgttca ttcctatagc gaattggaaa ggtcgaccgt
     3601 gaattggata ctgctcgaaa tggagcaaac cgaaaatctg agcgccggtg aggtcattct
     3661 ggatggtttg accaatgtgt tgtcagaacc ggaattcatg gaatcggatg aagcccgcag
     3721 ggtattaaaa ctgatcgagg agcgcactct cttacaagac ctcatttctc gttccggctt
     3781 taaagaccaa accaatggcg tgcaggtgtt gatcggtgga gagggcacct gggatgaatt
     3841 caaacagatg tccatcatct tgggaaaata cggtttgcct ggcgtagcca ccggcaccct
     3901 gggcgttttt ggacccatcc gcatgtctta cggtcattcg atctccattg ttcgtttcct
     3961 ttccagtttg ctcagcgatt tcgtcgccga acaatggtaa tttcaaacaa ggattttcat
     4021 tatgacagag aaagataagc acgaagaacc aatacaggaa gagaaaaaga ctcgtcccgc
     4081 cgaagcaggg gaagccatga aagaagaaaa agaaccccaa gaaggctaca ctgtcgaagc
     4141 ggaaaagcaa gaagagatgg tcaccatccc tctgaaaagc atggaagaac agttaaaaga
     4201 gatcgatgag ttaaaagacc aggtggctac ctattctgac ggatggaagc gcgaacgggc
     4261 tgatttcgac aattaccgaa aacgcgttct gcgcgatcag gagcaagaaa aacaaaatct
     4321 gacgatcgag atcgtcaaaa agtacctggt attgtatgat gacctgggtc ttgctttaaa
     4381 gaacgctcct acagagccag ggacaaaaca atgggttgag gggatcgcgt taattttgaa
     4441 gaaaatgcaa aaaatcttgg aggatgaagg aatagagcca atcaacgtag acaaggtgga
     4501 ttttgatccg caatttcacg aagcaatttc acatgaagaa gatcctgatt acaagagcgg
     4561 gcagatcatt gaagtggtca aacccggtta cagaataaat aatcgcatta tcagacctgc
     4621 actggtaaga gttgcaaaat aggaggcata gaatgggaaa aattatagga attgatctgg
     4681 gaacaacaaa ttcggtagtc gcagtgatgt cgggcggaga acccatcgtc atccccactg
     4741 ctgaagggga acgcctgtgc ccgtcagttg tggctattaa taaaaatggc gagagaattg
     4801 tgggtagaac tgcccgcaac caggctgtgg tgaacccgga aaacaccatt ttttcgatca
     4861 agcgcttcat gggacggaaa tatgatgatc ctgaagttca gcgcaccatc aaaagagttc
     4921 cctataaagt gaccaaagcg cctaacggag atgtgcgtgt cgtaatgggt ggcaaggagt
     4981 attcaccgcc agaaatttct gccatgattc tcgccaaatt aaagaaagac gccgagacat
     5041 accttggcga aaccgttacc caggcggtca tcacagttcc ggcatatttc aacgacgcac
     5101 agcgcaacgc caccaaagat gccggtaaga tcgccggatt ggatgtgctg cgcatcatca
     5161 acgagcccac cgcatcctct ttagcttatg gtctcgacaa gaaaaagaac gaagtgatcg
     5221 cggtgtacga cctgggtggc ggcaccttcg atatatccat tttggatgtg ggagaaggcg
     5281 tcttccaggt tcgctcaacc aacggcgata cctttctggg cggggatgac ttcgatcagc
     5341 tcgtcatgga ttacctgatc gatgaattca aacgcgacca gggtattgac ctgcgcaatg
     5401 atcgtcaggc gttacagcgt ttgaaagaag ccgctgaaaa agccaagatc gagctttcaa
     5461 cggtcatgga aactgagatc aatctgcctt acatcacagc cgatgccaat ggtccaaaac
     5521 acatgctcat ccggctcagc cgtgcgaagc tggaacagct aaccaacgaa ttgattgaaa
     5581 agaccatccc gccggttgag aaagctttga gtgatgcctc tttgaaacca agcgatatca
     5641 atgaaatcgt tctggtgggc ggtatgtcca gaatgcctgc agtgcgtgag atcgtgcgcc
     5701 agaaatttgg caaggaatcg cacaagggtg tcaaccctga tgaggtggtg gctattggtg
     5761 ctgccattca agccggtgtg ttaggtggag atgtcaaaga tatcctttta ctggatgtaa
     5821 cccccttgac gctctccgtt gaaacactgg gtggtgtggc aaccccattg atcgagcgca
     5881 acaccaccat tccaacccgc aagagccaga tcttttcaac agcctccgat agccagaccc
     5941 aggtgcagat tcacgtcact caaggcgaac gctcgatggc agccgataac aaatcgctcg
     6001 ggcaattcat cctggatggc attcccccag ccccccgtgg cgtcccgcag gtcgaggtca
     6061 ccttcgacat tgacgccaac ggtattctta acgtaaccgc gcaggacaaa gccaccgggc
     6121 gcagccaaaa gatcaccatt acggcttctt ccggtttgac caaagaagag atcgatcgca
     6181 tgaagaaaga agcagaatcc tttgccgatc aggataacaa acgcaaagag gtcattgaag
     6241 ctcgcaatat tgcagacaac actatttaca ctaccgagaa gaccctgcgt gaaggtggcg
     6301 ataaagttcc ggctgatgcc aagagagagg cagaagacgc catcaactct ctgcgcaaag
     6361 ccacggaagg cgacgatatt gcagaaattc gcaaacagac cgagtcgctc gggcagatct
     6421 tgcagaagat cggagccagt atgtatcaac agcccggcgc tgcaggtcca accggtcaac
     6481 ccggtcagga tgcaagcgct tcagatgatg ggcaggggca gaataaacca gatgatgggg
     6541 aagatgttgt tgacggtgaa ttcaagaatg tctagctggt aatttctagt acaatagata
     6601 gcgttttggg gttcgaagac cccaaaacgc tttttcgttc agcacacgga tcatcaaatg
     6661 gcagaaaaaa gagattatta cgaaattttg ggagtcccca agaatgcgtc tgcagaagac
     6721 ataaaatccg cattccggaa attggcgcgc caatatcacc cggatgtcaa tcacgaaccg
     6781 aacgcagaag aaaaattcaa agagatcaac gaagcttttg ctgtcttagg tgatcctgaa
     6841 aaacgtgctg cgtatgatcg ttttggattc gccggtgtga gcggcaacgg ggcacagtgg
     6901 gatgcttcta ccattgatcc atttgagatc ttcgaacaat tttttgggtt cggcggaata
     6961 gggggcagcg cccgccggcg caactctccg cggcgtggtg aagatattgt cacctcactg
     7021 gtattaaatt ttgaagaagc cttcacgggg gtcgacaaag atgtcgccat tacccgcgaa
     7081 gaagtgtgcc aggtctgcaa agggaccggt tcagaacccg gcacc
//
LOCUS       DAOSDS010000099         7104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3860_length_7104_cov_3.811037, whole genome shotgun sequence.
ACCESSION   DAOSDS010000099 DAOSDS010000000
VERSION     DAOSDS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3860_length_7104_cov_3.811037"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="rpe"
                     /locus_tag="PLL88_09650"
     CDS             complement(<1..500)
                     /gene="rpe"
                     /locus_tag="PLL88_09650"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOJ01869.1"
                     /translation="MKKIINASILNADFGSLKDQLDQAIAAGIDWVHLDIMDGHFVPN
                     ISMGPAIAQTCRQLTTLPIDAHLMISNPNDFIPIFAKAGVNLISVHIESDAHIHRTLQ
                     NIKNHGCQAGIVLNPGTPADSISAVLDMVDLVLVMSVDPGFGGQKFIPSVLSKIMQIR
                     KIIDAQ"
     gene            complement(557..1477)
                     /locus_tag="PLL88_09655"
     CDS             complement(557..1477)
                     /locus_tag="PLL88_09655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOJ01870.1"
                     /translation="MNLEYREVDFRNTVLDKRFLVEKRIDAGGMSLLYYGKDLSTRCR
                     IVIKICRSEEERGAFEQESRILRSLHHPHIQRIIGSGSIDGYPYYATPFYGSQNFREF
                     LSDKTILPEMDLLGYFLQISAAVAYLHKRKILHNDLKPQNIIVQDENCLTLSDFGLAC
                     RAPLRKYIAAEQKNIWGSPVYLAPELPQGFSPSFASDVYALGVILFILALGFPPFYHD
                     DLDTLIRMHQTEEPPHPSDLSDSISKELERIMLRAIAKSPAQRYTNAGKLHAAIQTYI
                     QKDRQRIEKNTLCRHIENHFSNQEKTRHIP"
     gene            1527..1982
                     /locus_tag="PLL88_09660"
     CDS             1527..1982
                     /locus_tag="PLL88_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01871.1"
                     /translation="MKKSTLPFWLGTFLGIGSFILALIVIFSFPDTKDDALPTAIVKI
                     TPYVPTATNSMVEQTEIASSQEQATALPGVFATNMRVRVSNTGGDGLKIHTEAGIESD
                     TLTIASENSLWMIVEGPTIKESRIWWKIQSLDSAIAGWAVQDYLTAEYQ"
     gene            1998..2945
                     /locus_tag="PLL88_09665"
     CDS             1998..2945
                     /locus_tag="PLL88_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ01872.1"
                     /translation="MEIDAVNELALKVMDNLEKVIIGKREVLEMVVLGLFCRGHILIE
                     DVPGTGKTMLAKSLAISMGGKFSRIQFTPDMLPSDVTGISMYNQASQTFEFRPGPVMA
                     EIVLADEINRATPKTQSALLEAMEERQITVDGKTHKLPDGFLVFATQNPIEYEGTFPL
                     PEAQLDRFLMRIHIGYPIFPDEMRILESQSHSHPIESLQSVTSSDEIQAAQQAVKNVL
                     VSEPVKKYIVNIVETTRQDKDIYLGASPRGSLGLYRAGQVYAILRGREYVTPDDIKKV
                     VIPILAHRIVLHPAARIKDLNKDALIGEILERVEVPVHA"
     gene            2948..4273
                     /locus_tag="PLL88_09670"
     CDS             2948..4273
                     /locus_tag="PLL88_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ01873.1"
                     /translation="MKTKRRFYFLLVGIAGFLALWIIFKDDLFLNILILILSLAIISY
                     LSARIALKGIILQRTSRFQKMNVGDYFEDHFDIENTHPLWKQWLEIRDYSGISGNAEE
                     ARILAGIDARKVRTFNTYTFLRKRGEFTLGPMRLVSGDFFGFFELSRIVENPKRLVVL
                     PRIVKLDHFAYLPAYLSGGKELKVASPETTPFAAGVREYFPGDTLRRIHWPTTARKAK
                     LMVKEFDKDPQMNTWILVDCAAENHFAPKETDPFSHPSDYWSVRRQARYELPCCTFEY
                     AVTAAASITKFFIEHNFGVGVISSNHQGQVLVSDHGYRQLGKALDILASLQPDGNIPF
                     DVFVMDHIKRINRGNTVILISTLQNAQMLNIIDLVMARGLAGIYVCVHKASFLQDKNT
                     PPAYDWFQAEIQMRAIPFFQIHYTADVKECLENEIAMRPSPAARHPERT"
     gene            complement(4344..6854)
                     /gene="lon"
                     /locus_tag="PLL88_09675"
     CDS             complement(4344..6854)
                     /gene="lon"
                     /locus_tag="PLL88_09675"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOJ01874.1"
                     /translation="MEILELFADEERDFNEMVLAMNMDDLTNDEGLVEIENKAGDQND
                     QAEFPEEVPILPLRGLVVYPQTAVPLTIGQTRSVRLVDEVVKSNRMVGLIASKDPSVD
                     EPEPAMLYQYGTLAIIHRLFRAPDGTIRLLVQGTTRFKVKEYTETRPYLKALVTPAPE
                     KIETGLKIEALARNVRDQFSQIAELVPSIPGEIVISINALQDPLQTVYNIANLQRMEV
                     EDAEQILELDSTTEKLRKLVGILTRESEVLNLGRKIQNEAREEIEKVQREYYLREQLK
                     AIQKELGESDEQGAEVSEFREKIQKAGMSEEALKMAVRELDRLSHLPTAAAEYGVIRT
                     YLDWLVSLPWSKTTTDDLDIRHAKAVLDRDHYGLDDVKKRILEYLAVRKLRLQRKAEL
                     SSQEYHDSIRQEREGVILCFVGPPGVGKTSLGKSIAAAMGREFIRASLGGTRDEAEIR
                     GHRRTYIGAMPGRIIQSLRRVESCNPVFMLDEIDKLSSDFHGDPSSALLEVLDPEQNS
                     EFRDNYLEVAFDLSQVMFITTANTIETIPAPLLDRMEIIQLSGYTENEKLQIAKSYLI
                     PRQLKENGLLQREITFEDEAILTIIREYTRESGVRNLERNIGGVLRKIVTRKFEKKRM
                     MKNVTARNVHDMLGRPIFKPAEEIMLRTSIPGVATGLAWTPTGGDILFIEATRMPGNQ
                     QFKITGSIGDVMEESAQAALSYVRAHAAEYNIDPNFFEKSDIHLHVPAGATPKDGPSA
                     GVTMTTALISLLTQKSIRPEVGMTGEITLRGQVLPVGGIKEKMLAAHRAGLKTIFLPK
                     ENKGDLNELPDEVKKSIQFIPVENVKQILKKAMVNGSK"
     gene            complement(6888..>7104)
                     /locus_tag="PLL88_09680"
     CDS             complement(6888..>7104)
                     /locus_tag="PLL88_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ01875.1"
                     /translation="NISGTVLTIDGTRWESYPDCAFHQMEIPSGDFSSSVEIPSPIDE
                     GSITAVYENGFLHITLPKAKVKQIKVN"
BASE COUNT         1703 a         1731 c         1830 g         1840 t
ORIGIN      
        1 tcttgtgcgt cgattatttt tctaatctgc atgatttttg acagcacgga aggaatgaac
       61 ttctgaccgc caaagccagg atcaacgctc atcaccaaaa ccagatccac catatccaga
      121 accgcggaga tggaatctgc cggcgttccg ggattgagaa cgattcccgc ctggcagcca
      181 tggtttttga tattctgcaa tgtgcgatgg atgtgcgcat cggattcaat gtgcaccgag
      241 atcagattca ccccggcttt cgcaaaaatg gggatgaaat cattcggatt ggagatcatc
      301 agatgggcgt ctatggggag ggtggtaagc tggcggcagg tttgtgcgat ggcgggtccc
      361 atagaaatat tgggaacgaa atgaccatcc atgatgtcca ggtggaccca atcgattccg
      421 gcagcgattg cttgatcaag ctgatctttt aaacttccaa aatcggcatt caggatcgag
      481 gcgttgatga tctttttcac gggcattctc caagttctat ttcgttaatc ttactacaag
      541 cagaggtcgg ttatgcctat ggaatatgcc tggtcttttc ctggttggaa aaatgattct
      601 caatgtgtct gcacagagtg tttttttcta ttctctggcg atccttttgg atataggttt
      661 gaatggctgc gtggagtttt cccgcattgg tatagcgttg tgcaggcgat tttgcgattg
      721 cgcgcagcat gattctttcc aattctttgg agatcgaatc ggatagatcg ctcggatgtg
      781 gcggttcttc agtttggtgc atacggatca gtgtatccaa atcgtcatga tagaagggag
      841 gaaagccaag ggcgaggata aacagaatga cccccagtgc gtacacatca gaagcaaaac
      901 tggggctgaa tccctgcggg agttcaggtg ccagataaac gggcgatccc cagatgttct
      961 tttgctccgc cgctatgtac ttgcggagcg gtgcgcggca ggcaagccca aaatcgctca
     1021 aggtcaagca gttttcatct tgcacaataa tattctgggg tttgagatcg ttgtgcaaga
     1081 ttttgcgctt atgcaggtaa gcaactgccg cgctgatctg taggaaatag cccagcagat
     1141 ccatttcagg cagaatggtt ttatcagaaa ggaattctct gaaattttgt gacccgtaga
     1201 agggtgtagc ataataagga tagccatcta tagagcccga ccctatgatc ctttgtatat
     1261 gcgggtggtg aagcgagcgc aatattctac tctcctgctc gaaagcgcct ctctcttctt
     1321 ccgatctaca aattttgatc acaatacggc accgggttga aaggtctttt ccatagtata
     1381 agagcgacat tccccctgca tcaattcgct tttccaccag gaaccgctta tccaatactg
     1441 tgttgcgaaa atcgacttct ctatattcca agttcataat aatcaggtaa tatagttaat
     1501 aatattccat atatggaaag aatagaatga aaaaatctac attaccattc tggcttggaa
     1561 cgtttttggg gatcggttct ttcattctgg cattgatcgt gattttttct tttccagaca
     1621 caaaagatga cgctctgcca acagcaatcg tgaagatcac accctatgta ccgactgcca
     1681 caaattccat ggtggaacaa accgagattg catcgtcaca ggaacaggcg actgcgctgc
     1741 cgggggtttt tgcaaccaac atgcgcgtgc gcgtttccaa tactggaggg gatgggctga
     1801 aaatccacac ggaggcgggc atagaatcgg acaccctgac aatcgcttct gagaactccc
     1861 tgtggatgat cgtagaagga cctaccatca aagaaagccg catctggtgg aagatccaat
     1921 cgctggattc agccatagct ggttgggcgg tgcaggatta tctgacggct gaataccagt
     1981 aacgcaaaga ggaagctatg gaaattgatg cagtcaacga gttggcgctc aaagtcatgg
     2041 ataatttgga aaaggtgatc atcgggaaac gcgaggtgct cgagatggtc gtgctggggc
     2101 tgttttgccg cggacatatt ctgatagagg acgtgcccgg cactggtaag accatgctgg
     2161 cgaaaagcct ggcaatcagc atgggaggaa aattcagtcg catccaattc acccccgata
     2221 tgctgcccag tgatgtgacc ggcatatcca tgtacaatca ggcaagccag accttcgaat
     2281 tccgccccgg tccggtgatg gcggagatcg tgctggcaga tgagatcaac cgggctaccc
     2341 caaaaacgca atcggcactg ctggaagcca tggaggagag gcaaataacg gtagatggga
     2401 agacgcataa attgccagat ggctttctgg tgtttgccac ccaaaaccca atcgaatacg
     2461 aaggcacctt tccactccca gaagcccagc ttgatcgttt tttgatgcgc atccacattg
     2521 gatacccgat ctttcctgac gaaatgcgca ttctggaatc acaaagtcat tcccatccca
     2581 ttgaatccct gcaatccgtg acctcaagcg atgagattca agcagcccaa caggcagtga
     2641 aaaatgtgct tgtttccgaa cccgttaaga aatatatcgt caacattgtt gaaaccactc
     2701 gccaggacaa ggacatttat ctgggagctt cgccgcgcgg cagtttgggg ctatatcgag
     2761 caggacaggt gtacgccatt cttcgaggca gggaatatgt cactcctgat gatatcaaaa
     2821 aggtggtgat acccatactg gctcaccgca tcgtgctgca cccagcagcc cgcattaaag
     2881 atctcaacaa ggacgctctg atcggtgaaa tattggaacg ggttgaggtt ccggtgcacg
     2941 cctgaatatg aaaaccaaac gccgttttta ctttttgttg gtcggaattg ccggctttct
     3001 ggcattatgg atcatcttta aagacgacct gtttctcaat attctgatcc tcattctttc
     3061 gctcgcgatc atcagctacc tatctgcgcg tattgcgctc aaaggcatca tcttgcaacg
     3121 aacctctcgt tttcaaaaga tgaacgtggg cgattatttc gaagatcatt tcgatattga
     3181 aaatacgcat cccttgtgga aacagtggct ggagatacgc gattattcgg gaatttcggg
     3241 gaacgcagag gaagccagaa ttctggctgg tatcgatgca aggaaagtcc gcactttcaa
     3301 cacctacaca tttcttcgga aacgaggtga attcactcta ggacccatgc ggttggtatc
     3361 aggcgacttt tttggctttt tcgaactttc caggatagtt gagaacccca aaaggttggt
     3421 ggtactgccg cgcattgtca agctagacca ttttgcatac ctgcctgctt atctcagtgg
     3481 tggtaaagag ctcaaggtgg cgtctcccga aacaacgcca ttcgcagcgg gagtgcggga
     3541 atatttcccg ggcgatacgc tgcggcgcat tcattggcct accaccgccc gtaaagcgaa
     3601 actgatggtg aaagaattcg acaaagaccc gcagatgaac acctggatac tggtagattg
     3661 cgcggctgaa aatcattttg cgcccaaaga aactgatcct ttcagccatc catcagatta
     3721 ttggagcgtt cgccggcaag ctcgttacga gttgccgtgt tgcacattcg agtatgcagt
     3781 gactgcagca gcgtcgatca cgaagttttt catcgagcac aattttgggg taggggtgat
     3841 ctcatccaat caccaggggc aggtgctggt ttcggatcat ggctaccgcc agcttggaaa
     3901 agcattggac atattggcgt cgttgcagcc ggatggaaat attccattcg atgttttcgt
     3961 tatggatcat ataaagcgga tcaatcgcgg caatacggtg atcttaattt caacattaca
     4021 aaatgcgcag atgctcaata tcatcgatct ggtgatggcg cgcggtctcg cagggatcta
     4081 cgtgtgcgta cacaaagctt ctttcttgca agacaaaaat acacctccgg cgtatgactg
     4141 gtttcaagcg gaaatacaga tgcgtgccat accattcttt cagattcact acacagcaga
     4201 cgtaaaagaa tgccttgaaa atgaaattgc tatgcgccca tcacctgctg cccgacatcc
     4261 agaaagaacg taaaacaaaa atgccctggt cggatagctg ttggttgccg agacagggca
     4321 ttttaagcaa gaatccttct ggatcatttc gatccgttta ccatggcttt tttcaatatc
     4381 tgcttcacat tctccaccgg gatgaattga atgctctttt tgacctcatc cggcagttcg
     4441 ttcagatctc ctttgttttc cttggggagg aaaatggttt ttaacccggc gcggtgagct
     4501 gccagcatct tttccttgat cccgccgacg ggcaaaacct gaccgcgcag ggttatttcg
     4561 ccggtcatcc cgacctcagg gcggatggat ttttgggtga gcagagagat caacgccgtg
     4621 gtcatcgtca ctccggcaga tgggccgtct ttcggtgttg ctccagccgg gacgtgcaga
     4681 tggatatctg atttttcgaa gaaatttgga tcgatattat attccgctgc atgcgcgcgc
     4741 acataggaca aagccgcctg ggcagattct tccatgacat cgccaatgga accggtgatc
     4801 ttgaattgct ggttgccggg catgcgcgtg gcttcgatga agaggatatc tccgcctgtt
     4861 ggcgtccagg caaggccggt ggcaacgcct ggaatggatg tgcgcaacat gatctcctcc
     4921 gccggtttga agatgggcct gccaagcata tcatgcacat tcctggcggt cacatttttc
     4981 atcatacgtt tcttctcaaa cttgcgcgtc acgatcttgc gcagcacacc cccgatattg
     5041 cgttcgaggt tgcgcacccc cgattcgcgc gtgtactcac ggatgatggt gaggatggct
     5101 tcatcctcga acgtgatctc gcgctgcaga agcccgttct ctttcaactg gcgtgggatc
     5161 aggtaacttt tcgcaatctg caatttttca ttttcggtgt agccggaaag ttgaatgatc
     5221 tccatgcgat ccagcagagg ggcgggaatg gtttcgatcg tgttagccgt ggtaatgaac
     5281 atgacctgag aaagatcgaa agctacctcc agatagttgt cacggaattc cgaattttgt
     5341 tcggggtcca atacctccag caaagcggaa gatggatctc cgtggaaatc ggaagataat
     5401 ttatcgatct catccagcat aaagaccggg ttgcacgact caacgcggcg cagcgattgg
     5461 atgatgcgcc ctggcatggc gccgatatag gtgcggcggt gcccgcggat ctcggcttcg
     5521 tcgcgtgtgc ctcccagcga agcgcggata aattcgcgcc ccatagccgc cgcgatggat
     5581 ttgccaagcg aagtcttgcc aacgccgggt ggacccacga agcacagaat gacaccttcg
     5641 cgctcctgac ggatggaatc atgatattcc tggcttgaaa gttcggcttt gcgttgcagg
     5701 cgcagtttgc gcactgccag atactcgagg atgcgctttt tcacgtcatc cagcccataa
     5761 tgatcgcgat caagcacggc tttggcatgc cgaatgtcga ggtcatctgt tgtggttttg
     5821 gaccatggca gcgaaaccag ccagtctaaa taggtgcgga tgacgccata ttccgcagct
     5881 gcggtgggca gatgcgaaag gcgatccagc tcacggacag ccattttcag cgcttcttcg
     5941 ctcatgccgg ctttctgaat cttttcgcga aactcgctta cctcagcgcc ctgttcgtca
     6001 gattcgccca gttctttctg aatagctttg agctgttcgc gcaggtaata ttcgcgctgt
     6061 accttttcaa tctcttcgcg cgcctcattc tggatctttc tgccaaggtt gagcacctca
     6121 ctttcgcggg tgagaatgcc gaccagcttg cgcaattttt ctgtggtgga atccagttcc
     6181 aggatctgtt ccgcatcttc cacttccatg cgttgcaagt ttgcgatgtt gtaaacggtt
     6241 tgcagagggt cttgcagggc gttgatcgaa atgacgatct caccggggat cgatggcacc
     6301 aactcggcga tctggctgaa ctggtcgcgc acattgcgcg ccagggcttc gatcttcaaa
     6361 ccggtctcaa tcttttcagg ggctggtgtg accagggctt tcagataggg tctggtttcg
     6421 gtgtattcct tgaccttaaa gcgggtggtg ccctgtacca gcaggcggat cgtgccatct
     6481 ggtgcgcgaa acagacggtg gatgatagcc aatgtgccat actggtagag catagctggt
     6541 tcgggttcgt caacgctcgg gtctttcgag gcgatcaagc ccaccatgcg attggatttc
     6601 accacctcat ctaccaggcg gacggagcgc gtctgcccga tggtcagtgg gaccgcggtt
     6661 tgcggatata ccaccagacc gcgtagcggt agaatgggca cttcttcggg aaattccgcc
     6721 tgatcgttct gatcgcccgc tttgttttct atttctacca gaccttcgtc gtttgtcaga
     6781 tcgtccatgt tcattgccag caccatttcg ttaaaatctc tttcttcatc agcaaacagc
     6841 tcaagaattt ccatgatatt ggttttccag cgagaagcgg gcataattta gttcaccttg
     6901 atctgtttta cttttgcttt gggcagggtg atgtgcagaa aaccattctc gtacactgct
     6961 gtgatgctgc cttcatcaat gggggaggga atttccacag aggatgaaaa atctccgctg
     7021 gggatctcca tttgatgaaa agcacaatca gggtaacttt cccaacgtgt gccatcaatg
     7081 gtaagcaccg tcccgctgat atta
//
LOCUS       DAOSDS010000100         7098 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3865_length_7098_cov_4.340054, whole genome shotgun sequence.
ACCESSION   DAOSDS010000100 DAOSDS010000000
VERSION     DAOSDS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3865_length_7098_cov_4.340054"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PLL88_09685"
     CDS             <1..515
                     /locus_tag="PLL88_09685"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOJ01876.1"
                     /translation="SLDFPGHAIGGLSVGESKEDMLASIEVVNAVLPESKPRYLMGVG
                     TPYDLIESIRRGVVMFDCVLPTRLARHSAAQTLSGRMNLMNAQYARDERPIDETCTCY
                     ACRYYSRAYLRHLIRAREMLGATLISIHNIHTLVHLAHSLRQAILAGTFEQAATALLE
                     NIPLSERTNA"
     gene            512..2047
                     /gene="uppP"
                     /locus_tag="PLL88_09690"
     CDS             512..2047
                     /gene="uppP"
                     /locus_tag="PLL88_09690"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HOJ01877.1"
                     /translation="MTIIQSIILGILQGLTEFLPISSSGHLVILPYLLGWDIPAEQNF
                     SFDVLVQVGTLVAVIIYFRKDIADIFKGLGADMKAKKLGSTSESRIGWFLLLATIPAG
                     AVGLLIKDIVEQTFGSPNAVAFFLFGTALLLILAEVVGKKDRDLSSLRWFDAVWIGFF
                     QVLSLFPGLSRSGSCIAGGMIRNFDRKSAGRFSFLMAIPVMAAAGLLSVFDLFNIPNL
                     ASFLPVLLIGFVVAGVVGYFTISWMLNFVQNHSLFVFAGYCLFLGTAVLAFGFFFPAA
                     GSAIPSETPAVQEMPLPGSAVYIPELDWLAPAIATCMDETLDAPLPLLQSTGDYQPTE
                     LRFTFPFATVTDHYAYQVGTQSLQFTLNPANPLVALNRSDLSALLQGNYPSWRQFFQN
                     CASCSYSSAGSASFDAPITLYIYPPESPYQTALNQTLALTSGQMGSALYVPDAASLLA
                     ALQLEQGAAGFLPSHWIPSGTSTVSIIDLPAAALDLPILAVLDTEPDEQYRSFILCVQ
                     DSL"
     gene            2123..2971
                     /gene="proC"
                     /locus_tag="PLL88_09695"
     CDS             2123..2971
                     /gene="proC"
                     /locus_tag="PLL88_09695"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOJ01878.1"
                     /translation="MLSDLTIALIGPGVMAEAMLKGLLKQQLTSPQRILVAGPNAERN
                     QELQTTYGVQPFTDNRAAAAQADIVVLSIKPQRMEKVVSELKGALKPDAFILSIIAGA
                     SIKKIARLSGHERIVRCMPNTPAQIGEGITVWTASPQVSQAQQEMTCAFLNAFGKEIF
                     VEEEDYLDKATALSGTGPAYVYLFMEAMVDAGVHLGFPRRIAERLVVETLRGSVDYYD
                     NGKGDARHLANLRNQVTSPGGTSAEALYYLEKAGFRTAISRAIWAAYQRSRELGRDAE
                     TNPPEE"
     gene            2978..3988
                     /gene="add"
                     /locus_tag="PLL88_09700"
     CDS             2978..3988
                     /gene="add"
                     /locus_tag="PLL88_09700"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006846.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOJ01879.1"
                     /translation="MPSPYIDPNFPLVELHRHLDGNVRLETILDIGLKHNLGLPATTI
                     EGLRPYVQVVEPKPSILDFFTKFEWLTKAMVDTDACRRIAYENVEDACNEGIDYIELR
                     FSPLFMAQAHQLDPAGVVEAVADGIAAARQAFPTKVNLIGIISRTYGVEACWQELNAL
                     FTRKDDIVALDLAGDELHFPGELFVEHFKKARDAGWHACPHAGEVLGAESVWQALREL
                     GAERLGHAVHAVSDPALLDYLAEQRVGVESNLTSNVQTSTVPDYASHPLKLFLERGIP
                     ATINTDDPGISAIDISYEYTMAVEKVGLSVEQVRQAQRNALQVCFLSEEEKQALVNSK
                     RS"
     gene            4218..4562
                     /locus_tag="PLL88_09705"
     CDS             4218..4562
                     /locus_tag="PLL88_09705"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOJ01880.1"
                     /translation="MVTIVFYKDHRSRCPVLDFINDLPVLEQVKIRNTLRLLREFGTQ
                     LGMPHARHIQGKLWELRPGGIRLFYFTYIEEQFVILHGYRKQSKKTPEKEIKIALRRM
                     EELLSEGTNAKK"
     gene            4549..4839
                     /locus_tag="PLL88_09710"
     CDS             4549..4839
                     /locus_tag="PLL88_09710"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ01881.1"
                     /translation="MRRNNLPRFEDWETEKLQNVAFEAAAKELEPGYQIARLRILRGL
                     TQAQLAEKVGTKQPSIARLENGSSLPSISFLTKLADALDARLEINLVSDTYK"
     gene            complement(4934..5062)
                     /locus_tag="PLL88_09715"
     CDS             complement(4934..5062)
                     /locus_tag="PLL88_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01882.1"
                     /translation="MPPKAGKKKVHKKLNKKHELALELVMKDTTDIQIVQQVGVSL"
     gene            complement(5423..5635)
                     /locus_tag="PLL88_09720"
     CDS             complement(5423..5635)
                     /locus_tag="PLL88_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01883.1"
                     /translation="MDACRMEPGEQDEQVPLLTACGNNREKKKLDDAPFLTIIRFANF
                     TKWKLVRNNSQRELYKYSFLCDLPED"
     gene            complement(5699..>7098)
                     /locus_tag="PLL88_09725"
     CDS             complement(5699..>7098)
                     /locus_tag="PLL88_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01884.1"
                     /translation="TRADSVITPLTLFPPGYSFLLSRVSLLTGDYLLTARIINAVCFS
                     LVICTITWGMLVLTKNALPALVSGVLVLASPVMLEDFTGMMSEALFFFLFYGSFFFTL
                     IAIRRQKDGWLIPAVLLTMLAPLVRYIGVVQCVFNPLLLLLFSRSAWKERMRKALLFA
                     SLSILPIASWLIASGGSSHGFGGRSIRLSAGLGEKLLAFFKESSAILQGWIPYFGYRV
                     NLIPDGTKVGIILCALGALLLLDLYFYWKKRKAEQAGILLQLVFSLLLYSILYFIVLA
                     FSCLFASVPPDLNGRMFSPLWPTFVLLGVCGLAYLLEQVPVRWKKTATVLMVLVILLP
                     LRFYALRGWADARELHENGYGYTARAIRNSAFLQALEELSEDIPLISNDAGLVLFYTN
                     RMPYELKPVPNYRLGDGNTSIDVLFSTQNAALILDYASMRNTYEDWSARLDALTAGLG
                     ISFQDDIGGVYSFPK"
BASE COUNT         1611 a         2085 c         1814 g         1588 t
ORIGIN      
        1 gttcgctcga tttccccgga catgccattg gcgggctttc ggtgggtgag tcaaaagaag
       61 acatgctggc ttccatcgag gtggtcaatg ccgtgctccc agagagcaag ccccgctacc
      121 tgatgggggt cggcaccccc tacgatctca ttgagagcat ccggcgcggg gtagttatgt
      181 tcgattgcgt tctccccacc cgcctggcgc gccacagcgc tgcccaaacg ttaagcggac
      241 gcatgaacct gatgaacgcc cagtatgcgc gcgatgaacg ccccattgac gaaacctgca
      301 cctgctacgc ctgccgttac tattcccgcg cctacctgcg tcacctcatc cgcgcccgcg
      361 agatgttggg cgccaccctc atctccatcc acaatatcca cactctggtg catctggcgc
      421 acagcctacg ccaggcaatc ctcgcaggca cctttgaaca ggccgccacc gcgttgctcg
      481 agaacattcc gctttcagaa aggaccaacg catgaccatc atacaatcca tcatcctggg
      541 catcctgcag gggctgaccg agttcctgcc catatccagt tccgggcacc tggtcatcct
      601 gccctacttg ctgggatggg acattcctgc cgagcagaat ttttcctttg atgtgctcgt
      661 ccaggtgggc acgctggtgg ctgtcatcat atattttcgc aaggacattg cggacatctt
      721 caaaggtctg ggtgcggata tgaaagccaa aaagctgggc agcaccagcg aaagccgcat
      781 cggttggttc ctgctgttag ccaccatccc agccggtgca gtcgggctgt tgatcaaaga
      841 tatcgttgag caaacctttg gcagccccaa cgcggtggct ttctttctct tcggcactgc
      901 ccttctgctc atcctggcgg aagtcgttgg caagaaagat cgcgaccttt ccagcctgcg
      961 ctggttcgac gcagtgtgga tcggtttctt ccaggtgctc tcgctctttc cggggctttc
     1021 ccgctcgggt tcctgcatcg ccggcggcat gatccgcaat ttcgaccgca agtccgccgg
     1081 gcgcttctcc ttcctgatgg caatcccggt catggcagcc gccggcttgt tgagcgtgtt
     1141 tgacctgttc aacatcccca acctcgccag ttttctgccc gtcctgctca tcggtttcgt
     1201 ggtggcgggc gtggtcggct atttcaccat ctcctggatg ctgaacttcg tgcagaacca
     1261 ttcgctcttc gtgttcgccg gatattgcct gttcctgggt accgctgtgc tggctttcgg
     1321 tttcttcttc cccgctgccg ggtcagccat ccccagcgag accccggctg tgcaggagat
     1381 gccgctgccg gggagcgcag tctacatccc cgaactggat tggctggcgc ccgccatcgc
     1441 cacctgtatg gatgagacgc tggatgcacc gttgccgctg ctgcaaagca cgggcgatta
     1501 ccagccgacg gaactgcgct tcaccttccc cttcgcgact gttaccgacc actatgccta
     1561 tcaagtcggg acgcaatccc tacagttcac cctgaatccc gccaacccgc ttgtggcttt
     1621 gaaccgatca gacctgagcg ccctcttgca aggcaactac ccctcctggc ggcaattctt
     1681 tcagaattgc gccagttgct cctattccag cgcgggcagt gcatctttcg acgcccccat
     1741 cacactctac atctatcccc ctgaaagccc ctatcaaacc gccctcaacc aaaccctcgc
     1801 attgaccagt gggcagatgg gcagcgccct ctacgtcccc gatgccgcct ccctgctcgc
     1861 cgccctgcag ttggaacagg gtgctgcggg cttcctgccc tctcactgga tcccctccgg
     1921 aacttccact gtctccatca tcgacctgcc ggctgccgcg ctcgacctgc ccatcctggc
     1981 agttctcgat accgagcctg atgaacaata ccgctccttc atcctgtgcg tgcaggattc
     2041 gctttaagaa cgcttccatt tccgcgcttt tcatatacaa tacttcaaac gaacggggtt
     2101 tcccggggaa tgaggttttt ctatgttatc ggatttgacc atcgcattga tcggacccgg
     2161 ggtgatggca gaagccatgc tcaagggatt gttgaaacaa cagctcacca gcccgcaacg
     2221 catcctcgtc gccggaccca acgccgagcg caaccaggaa ttgcaaacca cctacggcgt
     2281 gcagcccttc accgacaacc gtgctgccgc cgcgcaagcc gatatcgtgg tgctctccat
     2341 caaaccccag cgcatggaaa aggtggtgag cgagttgaag ggcgccctca aacccgatgc
     2401 tttcatcctt tccattattg caggcgcttc cattaaaaag atcgcccgtc tcagcgggca
     2461 cgagcgcatc gtacgctgca tgcccaacac ccccgcccag atcggggagg gcatcaccgt
     2521 gtggaccgca tccccgcagg tcagccaggc tcagcaggaa atgacctgcg cttttctgaa
     2581 cgctttcggc aaggagattt tcgttgagga ggaggactac ctcgacaaag ccaccgccct
     2641 ctccggtacc ggtcccgcct atgtgtacct cttcatggaa gccatggtgg atgccggggt
     2701 gcatctgggc ttcccgcgcc gcatcgccga acgactggtg gtcgaaaccc tgcgcggctc
     2761 ggtcgattat tacgacaacg gcaagggcga cgccagacac cttgccaatt tgcgcaacca
     2821 ggtcacttcg cccggcggca cctccgccga ggcgctctat tatctcgaaa aagccggctt
     2881 ccgcaccgcc atctcgcgcg ccatctgggc ggcttatcag cgctcgaggg agctgggcag
     2941 ggatgccgag accaaccccc cggaggaata gcctctcatg ccctcaccct acatcgatcc
     3001 caacttcccc ctggtcgagc tgcaccgcca cctggatggc aacgtgcgcc tcgagaccat
     3061 tctcgacatc ggcttgaagc acaacctggg tctacccgcc accaccatcg aaggcttgcg
     3121 cccctatgtg caggtggtgg aacccaagcc cagcattctg gatttcttca ccaagttcga
     3181 atggcttacc aaagctatgg tcgataccga cgcctgccgc cgtatcgctt acgaaaacgt
     3241 ggaagatgcc tgcaacgaag gcatcgatta catcgaactg cgcttcagcc cgctcttcat
     3301 ggcgcaggcg caccagcttg acccggcagg cgtggtggaa gcggtggcgg atggcatagc
     3361 tgccgcccgc caggctttcc ccaccaaagt gaacctgatc ggcatcatca gccgcactta
     3421 cggcgtggag gcttgctggc aggaactgaa cgccctgttc acccgcaagg atgacatcgt
     3481 cgcgctcgat ctggctggcg atgaactgca ctttccggga gaattgtttg tggagcattt
     3541 caagaaagcg cgcgacgccg gttggcatgc ctgtccgcac gccggtgagg tgctgggagc
     3601 tgagagcgtg tggcaggcgc tacgcgagtt gggcgccgaa cgccttgggc atgccgtaca
     3661 cgccgtttct gaccctgccc tgctggatta tctcgctgaa cagcgggtcg gtgtcgaatc
     3721 caacctcacc agcaacgtgc agacctcgac cgtgcccgat tacgcttcgc acccgcttaa
     3781 gctcttcctg gagcgcggca tccccgccac catcaacacc gatgatcccg gcatcagcgc
     3841 catcgacatt tcttatgaat acacaatggc ggtggaaaag gtggggttga gtgtggaaca
     3901 ggtcaggcag gcgcagcgga atgcgctgca ggtgtgcttc ttgagcgagg aagagaagca
     3961 agcgttggtg aatagcaagc gcagttgaca aaacttgaca aaagttgaca gtggcaaaaa
     4021 atgcacataa ccgttcctag ggaatgacaa aatttgccta tcagagcggc gggctgggaa
     4081 cgcacctaca aactgcaccg cctccatcac agtcttatca tctgcagcag ccgttccatg
     4141 tgcgctgccc aaatattttt ctagtttttt aggtcttgaa aattataaca tttttgttat
     4201 aatacatact ggcaaacatg gtaaccattg ttttctacaa agatcatcgc agtagatgtc
     4261 cggtattgga tttcattaac gatttaccag tactggagca ggtaaaaata cgcaacacct
     4321 tgcgtctgct gagagaattt ggaacacaat taggtatgcc gcatgccaga catatccagg
     4381 gaaagctatg ggagttgcga cctggtggaa tacgattatt ctatttcacc tatatagagg
     4441 agcaatttgt gattttacac ggctatcgaa aacaaagcaa aaaaacgcct gaaaaagaaa
     4501 taaaaattgc cttacggcga atggaagaat tactgagtga gggtaccaat gcgaagaaat
     4561 aatttacccc gatttgaaga ttgggagaca gaaaaactcc agaatgttgc atttgaagct
     4621 gctgcaaaag aactggaacc tggttatcag atcgcgcggt tgcgtatcct gcgtggttta
     4681 acccaggcac agctagccga aaaagtgggt acaaagcagc cctccattgc gcggctggag
     4741 aatggatcga gtttgccaag catttcattc ctgacaaaat tagcagatgc tctggacgcc
     4801 agacttgaga ttaatctggt atctgacacg tataaataat atactactct tctcttatcg
     4861 tttaccaaac tccgtctctt ccgccacctg ttggcataaa ggtcataacc tggttacgtc
     4921 cggtgttcac gctttacaaa cttactccca cctgctgcac gatctggatg tcggtggtgt
     4981 ccttcatcac cagctcgagc gccagttcat gtttcttgtt caactttttg tgcactttct
     5041 ttttccccgc tttggggggc atctagcata gaacactgcc cgcatgtttt tcaagctgtc
     5101 aaaagcggaa ccttttgtgc gtttgttccc gggatccaat ccctccactt gcacttcttg
     5161 cgtttgtgct gctgtcacct tttgtcaact tttgccactc cccctttcca tatatcctcc
     5221 ttattttgtg caaatttttc aacaagtaga aatacggatc cgttttttga aaattgtttg
     5281 tggatacact tcgtttgggt gagatccctc actgcgttcg ggatgacgtt gaaaataaga
     5341 aaaggtgcgt tggcaacgca ccctcgttct ctcccgtcat tccgaaggag tacagcaact
     5401 gaggaatctc tccatttccg tgttaatctt ccggcagatc gcaaagaaaa gagtatttgt
     5461 aaagttctct ttgcgaattg ttcctgacca acttccattt ggtgaaattc gcaaagcgaa
     5521 tgatggtaag aaatggcgcg tcgtcaagct ttttcttttc cctgttattt ccgcaagctg
     5581 tcaaaagcgg aacctgctca tcctgttctc cgggttccat gcggcacgca tccatgcaaa
     5641 accatcccgc gcaggatata tgaaaacggc agccgccttt tttaaagccg cttcttttct
     5701 atttgggaaa ggaatatacc ccaccgatgt catcctgaaa ggaaattccc agtccagccg
     5761 tcaaggcatc caggcgtgca ctccaatcct cataagtgtt gcgcatggaa gcgtaatcca
     5821 ggatcaaggc tgcgttttgg gtggaaaaga gcacatcaat ggaagtattc ccatccccca
     5881 atcggtagtt ggggaccggt ttcagttcat atggcatgcg gttggtataa aaaagcacca
     5941 gacccgcatc gttcgagatc agtgggatgt cttcggaaag ttcctccaac gcctgcagga
     6001 aagcggagtt ccgaatagcg cgggcggtat agccatagcc gttctcatgc agctcgcgcg
     6061 catctgccca accgcgcagg gcataaaagc gcaaggggag aagaatcacc aacaccatca
     6121 ggacggtcgc agtcttcttc cagcgcaccg gcacctgttc taacaggtat gccaacccgc
     6181 atacgccgag cagaacgaag gttggccaca gaggtgaaaa catgcgtccg ttgaggtctg
     6241 gcggcacgct ggcaaacaag caactgaacg ccagcacaat gaaatacagg atgctataca
     6301 gcagcaagga gaacaccaat tgcaagagaa tgcctgcctg ttccgctttc cgttttttcc
     6361 aatagaaata cagatcgagc agcaacaggg cgcccaatgc gcacaggata attcccacct
     6421 tggtcccatc cgggatcagg ttcacccggt acccgaaata ggggatccaa ccctgcagga
     6481 tggcggagga ttctttgaaa aaagcgagca gcttttcgcc caatccggca gacaggcgga
     6541 tggaacgccc gccaaaacca tgggagctac cgccggacgc gatgagccag ctcgcgatgg
     6601 gcaggatgga tagcgaggca aacagcaacg cttttcgcat tctttctttc catgccgatc
     6661 gagaaaaaag cagcagtagg agcgggttaa aaacgcactg taccactccg atatagcgca
     6721 ccagcggagc cagcatggtc aggagcacag cggggatgag ccatccatcc ttttgtctcc
     6781 ggatggcgat cagggtgaaa aagaaagagc catagaacag gaaaaagaac agggcttcgc
     6841 tcatcatgcc cgtgaagtcc tcgagcatca ccggcgacgc cagcaccaac actccgctga
     6901 caagggcagg cagggcgttt ttagtgagga ccagcatacc ccaggtgatg gtacagatca
     6961 ccaggctaaa acataccgca ttgatgatgc gcgctgtcag gaggtaatca cccgtgagca
     7021 gagatactcg acttagcagg aatgaatacc cgggtggaaa gagagttaag ggggttatca
     7081 cgctatccgc cctggtca
//
LOCUS       DAOSDS010000101         7082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3881_length_7082_cov_5.910630, whole genome shotgun sequence.
ACCESSION   DAOSDS010000101 DAOSDS010000000
VERSION     DAOSDS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3881_length_7082_cov_5.910630"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PLL88_09730"
     CDS             complement(<1..518)
                     /locus_tag="PLL88_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HOJ01885.1"
                     /translation="MEAYQAWQATIGQLQMNMSKATFDTWVRDAKFISQKDNIFTIGV
                     QNAYVRDWLENRLTGTVTKLLSSIMNAPQQVIFQLQGTTESLPSVENLQNIQATSAAS
                     HRTGNLLLNSRYRFDNFVVGPNNRMAHAASLAVANNPSQAYNPLFLYGGVGLGKTHLL
                     QAIGNHIREKEQ"
     gene            complement(948..2240)
                     /locus_tag="PLL88_09735"
     CDS             complement(948..2240)
                     /locus_tag="PLL88_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ01886.1"
                     /translation="MKQAAIIGAGIGGLTAAHELSKRGYAVTIYEKADHAGGLAGGFH
                     HHGWDWSVEFFYHHWFQSDKAILNLMKELGLSEKVRFLRPQTVMYHQGSFYPLDSVGA
                     VLTFPGFSFFEKMRFGLVTAYIRYLAGWQRLESTTAHTWLLKYYGKKIYSINFEPMLR
                     GKFGEYYQQVPMSWFWARFKARSSKLGTYVGGFQAFFDEFVQKLLQEQVSIQFNSSVE
                     KITQSENDRVGVQVNGHTHVYDKVIITTSPASFAALAPELPAEYRQKVFQSKSIGAIV
                     LLLSLANPLSKEKYYWYNLPKSKEFPFLSLVEHTNFLSPEYFGGDHLVYCGDYLPAEH
                     EYFTLSKDELIERFTPSLQKINQDFSKEWIKESWLFKQPYAQPIPFLNHSKKLLDIKT
                     PLKNVFLITMSQVYPWDRGTNYAVELVEKSLDQILANQ"
     gene            complement(2247..3236)
                     /locus_tag="PLL88_09740"
     CDS             complement(2247..3236)
                     /locus_tag="PLL88_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOJ01887.1"
                     /translation="MFWLGILISAVFLYLAIRGLDLKALWNTIKTIELIWLLPGILSY
                     FLGVWVRAARWSILLKGKYSPSPAHLFPSICIGYLGNNIFPARAGEFLRAYQLREKRA
                     IPMSTSLATIIVERIFDGLVMIIFILKGLPGFLGQLQNPLLSGLIQWILIAGSILFIA
                     AFILVLVSAVYPDKAKQVFDLVFLRVLPAKAHEKYHTLLNRFFEGMESLRSPLRVLSV
                     FALSLVVWIFETGLYWCTQRSFQLGIPFSQLLLLNGALNILSTIPSAPGYIGVFDAPG
                     ISLLNAMNVASENAAAYILTLHAALWLPITLVGLIFFIREGLQWSQIQSQKMK"
     gene            complement(3343..3642)
                     /locus_tag="PLL88_09745"
     CDS             complement(3343..3642)
                     /locus_tag="PLL88_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ01888.1"
                     /translation="MPVYVYHCNSCGIQFEKNQKFTDEPLRICPQCGKKTIHKVITAP
                     VSVIYKGSGFYSTDHRSSSGATSTARGAATTETAEATKPAEPAKEKKASPEKNKA"
     gene            3908..5587
                     /gene="hutU"
                     /locus_tag="PLL88_09750"
     CDS             3908..5587
                     /gene="hutU"
                     /locus_tag="PLL88_09750"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOJ01889.1"
                     /translation="MKNDGGWFMGAPRTVKAPRGTQLTCKSWLTEAPFRMIQNNLDPE
                     VAQRPDELVVYGGRGQAARNWDCFDAILESLKNLEDDETLLIQSGKPVGVFRTHSDAP
                     RVLIANSNLVPHWATQEHFDALAARGLIMYGQMTAGSWIYIGTQGILQGTYETLASLA
                     AQKGWHSLKGKFVLTAGLGGMGGAQPLAITMNEGVGLIVEIDPEHARWQKEIGYLDRV
                     SDDLEEALDLVQEALEKKIPRSIGLVANAADIYAGLLKLDILPDVVTDQTPAHDFLMY
                     VPNGISLEAANELRRRDPKEYTRLSTNAMTAQVEAMLAFQRRGAEVFDYGNNIRQRAF
                     DNGLKEAFDFPGFVPAYIRPLFCEGKGPFRWVALSGDKEDIYKTDQAIMELFPEDAHL
                     HRWLQMARERVPFQGLPARICWLGYGEREKAGLRFNEMVASGALKAPIVIGRDHLDSG
                     SVASPNRETEGMKDGTDAVSDWPILNALINAVNGATWVSFHHGGGVGIGYSQHAGMVV
                     VADGTPQAARRLSRVLTSDPGMGIIRHADAGYDQALAAAKDMGIKIPMLKK"
     gene            complement(5626..>7082)
                     /locus_tag="PLL88_09755"
     CDS             complement(5626..>7082)
                     /locus_tag="PLL88_09755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ01890.1"
                     /translation="WTIVSFGDDTFVELNQFAAMLICAISVYGVSRLLERSPKSAILN
                     AFLFLSYPIVVMQGTTTQTDLVTAALISCAFYFLLFGFHQKQFKYLAISSLGIALALG
                     SKQTAFFILPGYVLTFIFLWLKTRKEMPKGIRKFTVVACVFFLLFGSLTYIINISYYN
                     GFFGPPGSAESQFGFSSFQDIFEILKNNSLRLTYSAIDPSGLTYPIKNYFIKAKARLV
                     AGPLFVVGIDLESSAYVLTGHQFRYLSVPHLTEDEAWYGPVGFFLMALALITATIQGL
                     RKKDSEKSGLVLTFLIYTLCILIFRPGWDPYQGRYFLSVAVLTTPLIHLSFSKKPIDK
                     IVRIAIILLSISIIITTHLLNEGKPVAVFKNNPSLIRETIWDMDRNDRITIQNRSMSN
                     PLRDIQLHLPQGSIVGLCINSGIWDYPFFGSDFSQTIVPIYPRNLFVDEDWIRAEHID
                     YIIFNVRETLYEDVPDFLTLIYEYDNWKLFEVNH"
BASE COUNT         1884 a         1594 c         1837 g         1767 t
ORIGIN      
        1 ttctgctctt tttcgcgtat gtggttgccg atcgcttgca gcaggtgggt tttccccagc
       61 ccaacgccac catataagaa tagtggattg taggcttgtg atgggttatt ggctacagcc
      121 agactggcag catgagccat acggttattg ggtccaacca caaaattgtc aaaacgatag
      181 cgggagttca gcagaagatt gccggttctg tgagatgccg cgcttgtggc ttggatattt
      241 tgcaggtttt cgacggatgg aagcgattcg gtggtccctt gcagttggaa gatcacttgc
      301 tgtggcgcat tcatgatgct gctcagcaac ttggtaaccg ttcctgtcag acggttctca
      361 agccaatctc gaacatatgc attttggacg ccaatggtaa aaatattgtc cttctgggag
      421 atgaattttg cgtcacgcac ccacgtgtca aatgtggctt tcgacatatt catctgcaat
      481 tgcccgatcg tggcttgcca tgcctgatat gcttccatgt ctgttagtac tccttttctt
      541 aaaatttttt atgcgaaact aaccccttaa aaagccatat taaggatgag tcgtactggg
      601 agatgcttct ggttagaagc tttgattcct caattgggta agaactattc tagcaaaatg
      661 ccgggagatg ttcaagtaaa aaaagtgtcc ttaaggttaa aaaaaaagaa accttaagat
      721 ttcggaatct taaaaaatgt tttgaagatt tcagggagtc gatacatctg tagaacgtgc
      781 gttctccgcc actagtgctt gaggtgagga atgtacccca acctgtaagg aaagtgtacc
      841 caagagaacg caggcgatac ggtggcaaat ttcttaacct taaaaaacgc gagcaggaaa
      901 tgcggcgaat gtatggcata tttctgaaaa aatcagggga aatttgatca ttgattcgcc
      961 aaaatttgat ccagggactt ctcgacaagc tcaacggcgt agttcgttcc acggtcccaa
     1021 gggtaaacct ggctcatggt gatcaaaaac acgttcttca gaggggtctt gatatccaac
     1081 agcttcttgg aatggttcaa gaatggaatg ggttgtgcat atggctgttt gaagagccag
     1141 ctttctttga tccactcctt tgaaaaatcc tggttgatct tctgcaagct gggcgtgaac
     1201 cgttcaatca gttcatcttt gctgagcgta aaatactcat gttccgctgg caggtaatct
     1261 ccgcagtaca ccaggtgatc tccgccgaaa tattcgggtg agagaaagtt ggtgtgctct
     1321 accaatgata agaagggaaa ctcttttgat ttcggaaggt tgtaccagta gtatttttcc
     1381 tttgaaagcg ggttggctaa ggatagaagc aagacgatag ccccaatgct ctttgattga
     1441 aagacctttt gcctgtactc tgcgggcagt tcaggcgcca gggcggcgaa ggaggcgggg
     1501 gaggtggtga taatgacctt atcatacaca tgggtatgcc catttacctg aacacccacc
     1561 ctatcgttct cagattgcgt gatcttttca accgagctgt tgaattggat ggaaacctgc
     1621 tcttgaagaa gtttttgcac gaactcatcg aagaatgcct gaaagccgcc cacataagtg
     1681 cccaatttgc tggagcgcgc tttgaagcgc gcccagaacc aagacatggg tacctgctgg
     1741 tagtattcgc cgaattttcc gcgcagcatg ggttcgaaat tgatgctgta gatttttttg
     1801 ccgtagtact ttaaaagcca ggtgtgggca gtggtgctct ccagcctctg ccatcctgcc
     1861 aggtaacgga tatatgccgt tacaagaccg aagcgcatct tttcgaaaaa cgaaaagccc
     1921 ggaaaggtaa gcactgcccc aaccgaatcg agggggtaaa aactgccctg gtggtacatg
     1981 acggtttgtg ggcgcagaaa gcgcactttc tcagataagc ccaactcctt catcaaattc
     2041 agaatggctt tatcactctg aaaccaatgg tgataaaaga actctacgga ccaatcccag
     2101 ccatggtgat ggaaacctcc agccagccct ccggcgtggt ctgctttttc gtagattgtt
     2161 acagcgtatc ctcgttttga taactcatgt gcggctgtta aacccccgat accggcgccg
     2221 atgatggcag cttgtttcat gatccctcat ttcatttttt gtgactgtat ctggctccat
     2281 tgtagccctt cgcggatgaa gaagatcaac cctaccaggg tgatggggag ccataaagca
     2341 gcgtgtaagg tcaaaatgta agcggctgca ttttctgaag ccacgttcat cgcattcaat
     2401 aggctgatgc caggcgcgtc aaaaacaccg atatatccgg gtgctgaggg gatggtggag
     2461 aggatgttca gtgcgccgtt gagcaggagg agctgactga agggaatgcc taactgaaaa
     2521 gatcgctggg tgcaccagta caaacctgtt tcgaatatcc agaccaccag cgataatgca
     2581 aaaaccgaca gtacccgcag aggggatcgc aatgattcca tcccttcaaa aaagcggttt
     2641 aggagtgtgt gatatttttc atgtgccttg gcgggaagta ccctgagaaa gaccaggtca
     2701 aagacctgct tggctttatc aggatagact gcggaaacca gcaccaggat gaaggcggct
     2761 atgaatagaa tggagccggc gatcaggatc cattggatca aaccgctcag caatggattt
     2821 tgaagttgtc cgagaaaacc cggcagtcct tttaggatga aaatgatcat aaccaatcca
     2881 tcgaagatcc gctctacgat gatggtagcc agtgaggtcg acattgggat ggcgcgcttt
     2941 tcgcgtaact ggtaagcgcg cagaaattca cctgccctgg caggaaagat gttgttcccc
     3001 aggtatccga tgcaaatgga aggaaaaaga tgagccggcg agggagaata tttccctttt
     3061 aacaatatgg accatcgtgc tgctcgaacc cagaccccca aaaaataaga gaggatcccc
     3121 ggcagaagcc agatgagctc gatggttttg atggtattcc agagcgcctt gaggtcgagc
     3181 cccctgatcg ccagatataa gaagaccgcg ctgataagaa ttccgagcca aaacatccat
     3241 cgtttcattt tgttgattct atcatgaagc cctgtttcga ttcgaaccga caaaaaacca
     3301 gaaccgatca ggttctggtt ttctcatgaa gagtgtgaag aattacgcct tattcttttc
     3361 agggctagct tttttctctt tggcaggttc tgccggtttg gtcgcttcgg ctgtctcagt
     3421 ggtggcggcg ccgcgtgccg tggaagttgc tcctgagcta gaacggtgat cggtggaata
     3481 gaagccagag cctttgtaaa tcacgctgac cggagcggtg atcactttgt ggatggtctt
     3541 ctttccacat tgggggcaaa ttctcaaagg ttcatcagtg aacttctgat tcttttcgaa
     3601 ttgtattccg caactattac aatgataaac atatactggc atagcaacta cctcaaactt
     3661 tgtattatag ccatttcggg gtgctggcgc aagttttcga attgcctcaa ataaaaagta
     3721 accatgaagg ataacgttga ctcaaaaaag aaggtcctat ctggcaggga aaagatgcca
     3781 ggaagagtca atgctacccc tcgagttact ttttctatta aggtattacg ttgttctcca
     3841 tctggaacag gtacgggtga aaacaggaat ttcctctctt ctctctaaaa gattggatac
     3901 tataatcatg aaaaatgatg gaggttggtt catgggagca ccacgcacag taaaggcacc
     3961 caggggaacg caattgacct gtaagtcttg gttgaccgaa gccccttttc gcatgatcca
     4021 aaacaacctg gaccctgaag ttgcccagag accggatgag ctggtggtgt atggagggcg
     4081 cgggcaggct gccagaaatt gggattgttt tgatgccatt ttagagtcgt tgaagaacct
     4141 tgaagatgac gaaacgttgc tgattcaatc cgggaaaccg gtaggcgttt ttcgcaccca
     4201 cagcgatgcg ccacgcgtgc tcatcgccaa ttccaacttg gtcccgcatt gggctaccca
     4261 ggaacatttc gatgcgttgg cagcgcgagg cttgatcatg tacgggcaaa tgaccgccgg
     4321 ttcctggatc tatattggca cgcagggcat tctgcaggga acctatgaga ccctggcttc
     4381 actggcggcg caaaaaggct ggcactcact gaaagggaag tttgtgctga ctgccgggtt
     4441 gggtggtatg ggcggtgccc aaccgctggc tatcaccatg aatgaagggg ttggtttgat
     4501 cgtcgagatt gatccggaac acgcccgatg gcaaaaagag attggctatc tggacagggt
     4561 ttcagatgac ctggaagaag cgctggacct ggtacaggaa gcgcttgaga agaaaattcc
     4621 acgctctatc ggtctggtag ccaacgctgc cgatatctat gctggtctgc tcaaattgga
     4681 tatattgccc gacgtagtga ctgaccagac ccctgcccat gattttctca tgtatgtgcc
     4741 gaacggcatc agtctggaag ccgccaacga attgcgccga cgcgatccca aggaatacac
     4801 ccgcctgtcc accaacgcga tgacagcgca ggtagaggct atgctggcat tccagcgcag
     4861 gggggcggag gtcttcgatt atgggaacaa tatccgccag cgcgcatttg ataatggatt
     4921 gaaggaagcc ttcgacttcc caggcttcgt gccggcgtac attcgcccat tgttctgtga
     4981 agggaaaggt cccttccgct gggttgcgct atcgggcgac aaggaagata tttataagac
     5041 agatcaggcg atcatggaat tattccctga agatgcccat ctgcatcgtt ggttgcaaat
     5101 ggctagagag cgcgtcccgt tccagggact gccggcgcgc atctgctggt tgggttatgg
     5161 ggagcgcgaa aaagcaggct tgagattcaa cgagatggtt gccagcggtg cgttgaaagc
     5221 ccccattgtg atcgggcgcg accatctgga ttctggctcc gtggcttccc ccaaccgtga
     5281 aacggaaggg atgaaagacg gaacggatgc ggtcagcgac tggccgatct tgaacgcatt
     5341 gatcaatgcg gtgaacggcg ctacctgggt gtctttccat catggcggtg gggtgggcat
     5401 cggctacagc cagcatgccg ggatggtggt ggtggcagat ggaaccccac aagccgccag
     5461 gcgcttaagc cgggttctta cctctgaccc cgggatgggc atcattcgcc atgctgatgc
     5521 cggatatgat caagcgctgg cagcggcgaa agatatgggc atcaagatac ccatgttaaa
     5581 aaaataagac aatacagggt gggctttact ggcttgccct gtatattaat ggttcacctc
     5641 aaacagtttc caattatcgt attcgtagat gagagtcaag aagtcaggca catcttcata
     5701 aagagtttcc cttacattga aaatgatgta atcgatgtgc tctgctcgga tccaatcttc
     5761 atccacgaaa agatttctcg ggtatatcgg aacgatcgtt tgagaaaaat ccgatccgaa
     5821 aaatggataa tcccaaatac cggaattgat acataatccg acaatggagc cctgcggtaa
     5881 atgcaattgg atatctctta aggggttact catcgagcgg ttttgaatgg taattctatc
     5941 gttccgatcc atatcccaaa tagtctcgcg gatgaggctg gggttgtttt taaaaactgc
     6001 tacgggcttt ccttcgttca ataaatgcgt ggtgataata atggaaatgg ataacagaat
     6061 tattgcaatg cgtacaattt tatcaatcgg tttttttgaa aatgacagat gaatcagcgg
     6121 ggtggtcaaa acggccaccg acaggaaata acgtccctga tacggatccc atcctggtcg
     6181 gaatatgaga atgcagagcg tatagatcaa aaaagtcaac accaggcctg atttttccga
     6241 gtcttttttt cgcaatccct ggattgtcgc ggtgatcaag gcaagtgcca tcaagaagaa
     6301 gcccactgga ccataccagg cttcatcctc cgtgaggtga ggcacgctga gatagcgaaa
     6361 ctggtgccct gtcaaaacat acgcactgct ttcaaggtca atgccaacga caaacaacgg
     6421 ccctgcaaca aggcgtgctt ttgctttgat gaagtagttt tttatcggat atgtcaatcc
     6481 ggatgggtca attgcactat aggttaaccg tagcgaatta tttttcagga tttcgaagat
     6541 gtcctgaaaa cttgaaaaac caaactgcga ctctgcagaa ccgggaggac cgaaaaagcc
     6601 attgtaataa ctgatattga taatataagt gaggcttcca aataaaagga agaatacaca
     6661 agccacaacc gtaaatttgc gtattccctt gggcatctcc ttgcgcgttt tcaaccacaa
     6721 aaagatgaag gttagcacat aaccgggcag aatgaagaaa gctgtttgct tggatcccag
     6781 cgccagcgca atccccaaac tggaaattgc cagatacttg aattgctttt ggtggaatcc
     6841 gaaaagcaaa aaatagaagg cgcatgagat cagagcggcg gttaccaggt ctgtttgggt
     6901 tgttgtcccc tgcatgacga cgattggata agaaaggaac aagaacgcgt tgagtatggc
     6961 gctttttgga gaccgctcca gaagcctgct caccccataa acagaaatgg cgcagattaa
     7021 catggcggca aactggttta attcgacgaa ggtatcatct ccaaaggaaa cgatcgtcca
     7081 aa
//
LOCUS       DAOSDS010000102         7045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_3898_length_7045_cov_4.370243, whole genome shotgun sequence.
ACCESSION   DAOSDS010000102 DAOSDS010000000
VERSION     DAOSDS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7045
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3898_length_7045_cov_4.370243"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLL88_09760"
     CDS             complement(<1..337)
                     /locus_tag="PLL88_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ01891.1"
                     /translation="MNAISRPSLSEIVAGKIAGMILDEIYRSGYQLPAERDLVKQFGI
                     SRSTLREALKLLEDSALIEVRHGVGSFVAELTDASLLKAHEIADKIAGQNYPAEPESI
                     PIEEVPEGPK"
     gene            569..1669
                     /locus_tag="PLL88_09765"
     CDS             569..1669
                     /locus_tag="PLL88_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ01892.1"
                     /translation="MRAIYPFTAIVGQERMKRALILNAVDTRIGGVLIRGERGTAKST
                     AARALAALLPQVRVVSDCRFACDPDKPETWCTECRERADKGEELPTEIVRTPFIDLPV
                     SATEDRVVGTLNIEEAIRKGERHFEPGILASANRGLLYIDEVNLLDDHVVDILLDSAA
                     MGMNIVEREGISFTHPARFILVGTMNPEEGELRPQLLDRFGLSVEIHGISTSRERVAI
                     MQRHLAFEQNTEEFRQEWLEKEEELSKKIEKARSIVDQVTYTSRDLVAIAHMTSSMNV
                     EGHRADLVILKAARAQAAFEGRTRITDLDIGLVAELALLHRMRGGPMQMSEMDADELH
                     AKIEKLVGFKDPGQEEEPSSPSDKQESSKKKI"
     gene            1711..2634
                     /locus_tag="PLL88_09770"
     CDS             1711..2634
                     /locus_tag="PLL88_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ01893.1"
                     /translation="MPVPPQKAFEQVNANWWEGGQRIRVGEAFTPRRLDTPLDKLVRR
                     YGGRRSQTQTSRRRGRYIQARPANGDLSDIAFDATLRAAAPFQKKRADETDGLAFHVH
                     SDDFMKKIRVRRAANLILFLVDASWSMAVAERMSATKGAIVSLLTDAYQRRDKVGLIV
                     FQKDRATLILPPTNSIQLAQRALKNIPVGGKTPLSAGLAMASEVIKRELMVHPDVKPI
                     LIVMTDGAGNVAMGAYAPLEEAYQLAEKIAHEDINSVVINMETETYDQGLAYTLAKKL
                     DAPCLTIRELKAEHLYMAVRQVRDDIQRNNS"
     gene            2730..3377
                     /locus_tag="PLL88_09775"
     CDS             2730..3377
                     /locus_tag="PLL88_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01894.1"
                     /translation="MTVKLSVWELSKKYDSFLALAPTSLQLEAGKITTLSGPNGSGKT
                     TFLSCLSGLVCPSSGTVTVDNFDLYRDEVEFHRRLVFVPDVPRFYLELTAWEHLQFVA
                     MANGVMEDFDQRAKDLLEKLDLWEARNLFPHNFSRGMRLKMGLALAFIRPFGVILLDE
                     PTSALDTTGSNYLINELCQLRKRGVTILLSTHDEALINQLGDYHLKIHHGVIEMG"
     gene            3379..4986
                     /locus_tag="PLL88_09780"
     CDS             3379..4986
                     /locus_tag="PLL88_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01895.1"
                     /translation="MNAVHWLRARLEERELVYWLSILFYDKEDHSFSNRVYLCYLFLF
                     FGIWLFAMLTFLAQGGAIVLGIIDGNDPVRAATFLEILILGLWSLLASWQAMKRSPIV
                     FSEQDATLICQMPVNHRQVVMRWFFLPWLKSAIPFWLLTIVIGFSVAETAMAGSLGAN
                     SFSAYVTYGLRAWITIIPVQLALFSLQWVLGILRLRKDIAFRWLKWLVMATITFALVV
                     FLATVGNQFFDPLIRPGLNFALPIQAGFERGSLALPLGISWLLAIISLGILFWVSDLI
                     SLARAAQETQVEEFLNQASRFGLTSYVDELKTQRRLGVSHKPSHLPKFVGGGWMLIWK
                     DLIQSKRSWNSSSFFDWFTTFGIVAGFPLIPDVGSRIFAMAFWSIRLGITSVKRLRSD
                     LSCWQIIRQLPISHSRFLFLELSPAFFLSIIISSVGFLLSALVMKSIELGFVLLIPGI
                     TAGVIGIVAFDVIRRARSDYLLNGIAPDISVGGYVLGLLTATVPIILYTVLPGVSRLI
                     FPCLVSLTFGYFAFKIAARSYRNIDKR"
     gene            5129..6523
                     /locus_tag="PLL88_09785"
     CDS             5129..6523
                     /locus_tag="PLL88_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01896.1"
                     /translation="MNRKRLFFDQFWIRIFSAAIFLIFIFFAGCSGEVIFDTIFPRES
                     PSSTNEPADDLNVTATPPASETLTGEGNQTVATTPEATQEFTVEKNELVVWVPEEFSN
                     LVDSPAAEIFNQRIADFERQNPGTVVVLRVKASSGSGGILDSLRSTKVAATGALPHIA
                     LLSRVDMENAEGLALLLPVEEYSHDTSPSTYFSYAREISELDGIMYGFPFVGDALVGI
                     ADESMSDIDVPTWDEVRARKIPLYFAANSPQATFFISQYQSTNGLLVDEQGHGTFVDE
                     NFLTVLNTFDQNLKKGIFQETFNALSTNEDVWTQFRNGNARWVINWYSKAMQSDLSGL
                     QIFQIPSLDSEPYVSAKGWLWTIVDRDAEVNETILEFVTFFSDARFLADYSQAAGFLP
                     VLPPSLEIYEDGDTIVQLNTILTASHALPANDLILELGPVFRDATLMVMSQDYSMEEI
                     VEISQRQIEALQTK"
     gene            6520..6834
                     /locus_tag="PLL88_09790"
     CDS             6520..6834
                     /locus_tag="PLL88_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01897.1"
                     /translation="MKEEPEQTNRPWFNVTNILIAIILLFLLVFIVWNQSISEKIYLA
                     EEERALVMATSTPTAAVSLPIPAEFYSEPADTSGIILAGIILLMIVFISSFWKIRKLK
                     NK"
BASE COUNT         1824 a         1466 c         1765 g         1990 t
ORIGIN      
        1 gcttgggtcc ctcgggtacc tcttcaatgg gtatggattc gggttcagcc ggatagtttt
       61 gcccggcaat tttatctgct atttcatgcg ctttcaacaa actggcatcg gtcaattctg
      121 caacaaaaga gccaacgcca tggcgcactt cgatcagtgc agaatcttct aacaacttga
      181 gcgcttcgcg aagggtgctg cggctgatgc caaactgttt aaccagatcg cgttctgccg
      241 gcaattggta tccgcttcga taaatctcat ccaggatcat tccggcgatt ttccccgcaa
      301 cgatttcaga aagggatggt cgagaaatag cattcatatg cacctcaaga ttgtggtatg
      361 ttggtcatac cactgcacca tagtatagac gtattttctg cgaaagtcaa tatttgcgaa
      421 tggattcgtt gcacaatctt tatcttcata aagatatcac tgtttgattg ctactcagaa
      481 tcaggggatg atatctgatg aaagttatat attaaaattg ttgttcggag cgtgttcaaa
      541 tggtacactt actcggcgag gtacatacat gagagccatc tatcccttta cagccatcgt
      601 tggtcaggaa cgtatgaagc gtgcgttgat cctgaacgca gttgacacac gcattggtgg
      661 tgtgttgatc cgtggggaac gtggaaccgc taaatccacc gcagcacgtg ccctggctgc
      721 tttgcttccg caggtgcgtg tggtaagtga ttgccggttt gcctgcgatc ccgataaacc
      781 agaaacctgg tgcaccgaat gccgggaaag agctgacaag ggagaagagt tacccactga
      841 gatcgtgcgt acccccttta ttgatttgcc cgtatctgct acggaggatc gcgtggtggg
      901 aacgctgaat attgaagaag ccatacgcaa aggtgaacgg cattttgaac cgggaattct
      961 ggcatctgcc aatcgcggtt tgctctacat tgatgaggtc aatctgttgg acgaccacgt
     1021 ggtggatatt ttacttgatt cagccgccat gggcatgaat atcgtggaaa gggaaggaat
     1081 ctcctttacc catccagccc gttttatttt ggtcggaacg atgaaccccg aggaaggaga
     1141 gctgcgccct cagttgctgg atcgttttgg attgtctgtg gagatccacg gcatcagcac
     1201 ttcgcgcgag cgcgttgcga tcatgcagcg ccatcttgcc ttcgagcaga acactgaaga
     1261 gttcaggcaa gaatggttgg aaaaagagga agaactttca aaaaagatcg aaaaagcccg
     1321 aagcattgta gaccaggtga cttatacaag ccgtgatctt gtggcgattg cgcacatgac
     1381 ctcttcgatg aacgtagagg gacatcgtgc tgatctggtt attttaaagg ctgccagggc
     1441 gcaggctgct tttgaagggc gtacgcgcat cactgatttg gatatcggtc tggtggctga
     1501 actggcactc ctgcaccgta tgaggggtgg gccgatgcag atgagcgaaa tggatgcgga
     1561 tgaactgcac gcgaagatcg aaaaactggt cggatttaaa gaccccggtc aggaagaaga
     1621 gccatccagc ccttcggata aacaggaatc ttccaaaaaa aaaatataag cggcgaggag
     1681 accgctgaac cgacggctga agatagcgaa atgccggttc ctccccaaaa ggcatttgaa
     1741 caggtaaatg cgaattggtg ggagggtggt cagcgcattc gcgttggaga agcgtttacc
     1801 ccacgccgtc tggatactcc tttagataag ctggttcgtc gttatggtgg aaggcgttcg
     1861 caaacgcaaa cctctcgccg gcgcggacga tatattcagg cgcgccctgc caatggcgat
     1921 ctgagcgaca tcgcttttga tgccaccttg cgtgcagccg cgcctttcca aaagaaacgg
     1981 gcagatgaga cggatggctt ggctttccat gtgcattccg atgatttcat gaagaagatc
     2041 cgcgtgcgcc gggccgccaa cttgatcctg tttttggtgg acgcttcgtg gtccatggca
     2101 gttgccgaac ggatgagcgc gaccaaggga gcgatcgtat cgctccttac cgatgcctat
     2161 caacggcgag ataaagtggg gttgatcgtt tttcaaaagg atcgggctac cctcattctt
     2221 ccgccaacca actccatcca gcttgcgcaa cgcgctttga agaacattcc ggtcggcggc
     2281 aagaccccat tatccgccgg tttggcgatg gctagcgaag tgatcaaacg tgaattgatg
     2341 gtccatccgg atgtgaaacc gatcctgatt gtgatgaccg atggcgccgg taatgtagcc
     2401 atgggtgcct atgccccgct ggaagaagcg tatcaattgg ctgaaaagat agcgcatgaa
     2461 gatattaaca gcgttgttat taacatggaa acagaaacat acgatcaagg cctggcatat
     2521 accctggcga aaaagctgga tgccccctgt ttgacgatca gagagttaaa agcagaacat
     2581 ttgtatatgg cagtccggca ggtacgagat gatatccagc gcaacaatag ttgaaaaagt
     2641 cggtgatgca tactgagcgt tttatgataa ttcgaggaac tttgttgcaa aattaatatc
     2701 aatgaaaaaa attaaggaag gggctgacat tgaccgtaaa actttcggtt tgggaattaa
     2761 gcaagaagta tgattcattt ttagccttag ctccgacttc actccaatta gaggcaggaa
     2821 aaattacaac gctatccgga ccaaacggtt ctgggaagac cacgtttctt tcatgtttat
     2881 caggtttagt atgcccatct agtggaactg ttacggttga taactttgac ctgtatagag
     2941 atgaagttga atttcatcgt cggttggtat ttgttcccga cgttcctcgt ttttatctgg
     3001 agttaactgc ctgggaacac ctacagtttg tagcaatggc taatggtgta atggaagatt
     3061 ttgatcaacg ggcaaaagat ttgctagaaa aacttgatct atgggaggca agaaatcttt
     3121 ttccgcacaa tttttcacgt ggcatgcgtt taaagatggg tctggcactt gcttttatcc
     3181 gaccgtttgg tgtgattctc ctcgatgaac caacttcagc gctcgatacg acggggtcga
     3241 attatttgat aaatgaatta tgtcagttac ggaaacgagg ggtgaccatc ttattatcta
     3301 cgcatgatga ggctttaata aaccaattag gggattatca tctgaaaatt caccatgggg
     3361 tgatagaaat ggggtgaaat gaatgcggtg cattggttac gcgcgcggtt agaggaacgt
     3421 gagctcgttt attggttatc catcctcttt tatgataaag aggatcattc gtttagcaac
     3481 cgcgtttatt tgtgctacct ttttcttttc ttcggcatct ggctatttgc aatgttgaca
     3541 tttttagcac aaggcggagc tattgtatta ggcattattg atggaaatga tcctgttcgt
     3601 gcagctacct tcctagaaat tctaatttta ggattatgga gtctgcttgc ctcatggcaa
     3661 gcgatgaaac gaagtcccat tgtgttttcc gaacaggatg ccactttgat ctgccagatg
     3721 cccgtgaacc atcgacaagt ggtcatgcga tggttctttt taccatggtt aaaaagtgca
     3781 atcccttttt ggttattgac gattgtaatt ggcttctcgg tggcagaaac tgcaatggca
     3841 ggctccttgg gagcaaattc attttctgct tatgttacct atggacttcg cgcctggata
     3901 acgatcatac cagtccaact ggcattgttt tctcttcaat gggttttggg aatccttcga
     3961 cttcgaaaag atatagcgtt tcgatggttg aaatggttgg tgatggcaac gatcactttc
     4021 gctttggttg tatttttagc cactgtgggc aatcaattct ttgatccatt gatccgccct
     4081 ggtttgaatt tcgctttacc tatacaagca ggatttgaaa gaggcagttt agccctccca
     4141 ttaggtatca gttggctctt ggcaataatt tctttgggaa ttttattttg ggtttcggac
     4201 ttaatcagct tggctcgtgc tgctcaagaa acacaagtgg aggaatttct caatcaagcc
     4261 tcccgattcg gacttacttc ctatgttgat gaattgaaaa cacagcgtag attgggggta
     4321 agccataaac cctctcactt gcctaaattt gtgggtggag gatggatgtt gatatggaaa
     4381 gatttaatac aatcaaaaag aagttggaat tcgtcatcgt tctttgattg gtttactacc
     4441 tttggcattg tagcaggatt tcctttgatc cctgatgttg gaagtcgtat ttttgcaatg
     4501 gctttttgga gtattcgatt ggggataacg tctgtaaagc gcctgcgcag cgatttatca
     4561 tgctggcaaa tcattcgcca actccctatt tcacacagcc gatttctatt tttggaatta
     4621 agtccggcat tttttctatc gataatcatc agttcggtgg gtttcctgct tagcgcgttg
     4681 gtgatgaagt cgatagagct tggttttgtt ttattaattc caggaatcac cgctggagtt
     4741 attggaattg tagcattcga tgtaatcagg agagcacgaa gtgattattt attgaatggc
     4801 atagctcctg acatcagtgt tgggggatat gtgctgggat tgttaaccgc cactgttcca
     4861 attatcctgt atacagttct acctggggta tcaagattga tctttccatg ccttgtgagc
     4921 ttaacctttg gctatttcgc atttaaaata gcggctcgat catatcggaa tattgacaag
     4981 agatgaaaga aatcagatga aaaaaatggt gtatgcatag catcattccg gatttatttc
     5041 gcgttataat gcattccctt ggacatcgaa tggagaatga agtttcctaa atagccagca
     5101 taaagctgat acaataggaa attagtgcat gaatagaaaa cgtctatttt tcgaccaatt
     5161 ttggatcaga atattttccg ccgccatctt cttgattttt atctttttcg ccggatgcag
     5221 cggtgaggta atttttgata ccatctttcc aagagaatcc ccctcaagca ccaatgagcc
     5281 tgctgatgat ctgaatgtga cagccacgcc gcctgcctca gagactttaa cgggtgaggg
     5341 aaatcagact gtagcgacta cccccgaagc aactcaggaa tttacggttg agaaaaatga
     5401 gctggtggtg tgggtgcctg aagaattttc gaacctggtc gattcaccgg ctgcagaaat
     5461 cttcaatcaa cgaattgcgg atttcgaaag gcagaacccg ggtacggtgg ttgtgttgcg
     5521 ggtcaaggca tccagtggat cgggagggat cttggatagc ctgcgcagca ccaaagttgc
     5581 tgccactggt gccctgccgc acatagcgtt gctttctcgt gttgacatgg agaatgccga
     5641 aggactggcg ttgcttttac cggtggaaga atattctcat gataccagcc cctctaccta
     5701 tttttcttat gcccgtgaga tttcggaact ggatgggatc atgtatgggt ttccgtttgt
     5761 cggagatgct ttggttggga tcgcagatga aagcatgtct gatattgatg ttcccacctg
     5821 ggatgaggtc agggcgagaa agatcccact atactttgca gcaaattccc ctcaagccac
     5881 cttttttatt tcacaatacc aatcaaccaa tgggttactg gtcgatgagc aagggcatgg
     5941 aacatttgta gatgagaatt ttttaactgt cctcaacacc tttgatcaaa atttgaagaa
     6001 agggatattc caggaaacct tcaacgcatt gagcacaaat gaagatgtct ggactcaatt
     6061 tcggaatgga aatgcaaggt gggtgatcaa ttggtattcc aaagccatgc agagcgatct
     6121 aagcggatta cagatattcc agattccctc cctggattcc gaaccgtacg tttctgcgaa
     6181 aggttggctt tggacgatcg tggataggga tgcagaggtc aatgaaacta tcctggaatt
     6241 tgtaaccttt ttcagcgatg cgcgattcct ggctgattac agccaggcgg ctggcttctt
     6301 gcctgtgtta ccccccagtc ttgaaatcta tgaagatggc gatacgattg tgcaactgaa
     6361 taccattctg actgcatccc atgctttgcc tgccaatgac ctcattctcg aattaggtcc
     6421 ggtattccga gatgcaacac tcatggtaat gagtcaggat tattcaatgg aagagattgt
     6481 tgagatctca caaagacaaa tagaggcatt gcaaacgaaa tgaaggaaga acctgaacaa
     6541 acaaatcgcc cttggtttaa tgtaacgaat atactcattg ccatcatcct tttgtttctt
     6601 cttgtcttta tcgtttggaa tcaatccatc tcagaaaaaa tctacctggc tgaggaagaa
     6661 cgtgctctgg tcatggctac aagcacgccg accgcagcag tcagcttgcc cattcctgca
     6721 gagttttata gcgaaccagc agatacatca ggtattattc ttgctgggat aattttgctg
     6781 atgatcgtct ttataagctc gttttggaaa atcaggaaat tgaagaacaa ataaccaaaa
     6841 aaggttttcc tgtggaaaac cttttttggt tgttccgcgt atatggaatg tatatcgaat
     6901 gataaggaga aaggaatgat caaggaattt tcaaagaaaa aatgggtggg cattgtactg
     6961 gttgtttgtc tgatcacgtt gctgccggca ggcgcagtgt atgcggctga ttttagccat
     7021 gatggaattg ttggtgcagg cgagg
//
LOCUS       DAOSDS010000103         6849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4035_length_6849_cov_4.841036, whole genome shotgun sequence.
ACCESSION   DAOSDS010000103 DAOSDS010000000
VERSION     DAOSDS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6849
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4035_length_6849_cov_4.841036"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..1006)
                     /gene="eutJ"
                     /locus_tag="PLL88_09795"
     CDS             complement(122..1006)
                     /gene="eutJ"
                     /locus_tag="PLL88_09795"
                     /inference="COORDINATES: protein motif:HMM:NF011660.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HOJ01898.1"
                     /translation="MLGKVGEFDLTEYLTRAEQAILPSQIHNTSPAPVDDEPLYVGAD
                     LGTAYLVLVVLDKNLQPVAGEYQFAQVVKDGLVVDFFGAVDRLKEMKQRLEARVGRTL
                     TDAASSFPPGVPQAEVRATANVVEAAGLNCVGLVDEPTAANNLLGIQNGAIVDVGGGT
                     TGIALVENGKVIFSTDEATGGTHFSLVIAGARNISFEEAEELKKNAAMQKMLFPVVRP
                     VMEKVGNIITRQLDGRKITSITLVGGAACFPGMAEVVQDYTGIATHSPEKPLFVTPIG
                     IAMHHAQMAARNGDLSGR"
     gene            complement(1029..1355)
                     /gene="eutM"
                     /locus_tag="PLL88_09800"
     CDS             complement(1029..1355)
                     /gene="eutM"
                     /locus_tag="PLL88_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HOJ01899.1"
                     /translation="MAVNPELIALGMVETKGLVGSIEAADAMVKAANVTLIGSEYVGG
                     GYVTVMVRGDVGAVKAATDAGAAAAKRVGELVSVHVIPRPHNDVEMILPQKEKGSISG
                     RSTTAK"
     gene            complement(1377..2834)
                     /locus_tag="PLL88_09805"
     CDS             complement(1377..2834)
                     /locus_tag="PLL88_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOJ01900.1"
                     /translation="MPIDKDLLSIQEARDLAAQAYEAQLVWGKASQEQVDRVCAAMAE
                     AGYQASDRLGRMASEETGYGVAAHKKLKNELASRVLWDSIKDIKTVGVIKHDPEKRLY
                     EIAWPMGVVAALTPSTNPTSTVFAKALSAVKARDAIIVAPHPAAVNCSAEAARTLQQA
                     AENAGAPLGLINCMRTISLGGTQELMKHKRVRVILATGGEGMVKAAHSVGKPAYGVGP
                     GNVPVYVDRSADLEKAARYIVSSKSFDYSLICASEQSVVVDKPAAAQLAQRMQANGAY
                     FADERTADILRRILFNPDGTMNVATVGKSPQFLAQLGGFEVPADARIIVVRLNRVGAQ
                     EPLSREKLTTVLGWFEEEGWEDGCERCIELIHFGGRGHSLVLHATDQNVIMAFGLEKP
                     VFRVLVNTMGTLGAVGLTTGVMPSLTLGAGGEGGSVTGDNITVHHLFNIKRLAYETTP
                     PPEAAMVGSGAFSEPNQQELESIVRQVMLETLAKN"
     gene            complement(2848..3138)
                     /locus_tag="PLL88_09810"
     CDS             complement(2848..3138)
                     /locus_tag="PLL88_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOJ01901.1"
                     /translation="MLIARVIGTTVSTIKDPSLTGQKLLLCRQTDETGAPAGKPYVAI
                     DSVDAGVGDLVLTAHGSSARQTEITKNRPVDAVIMAVIDSLEVDGKVVFRKS"
     gene            complement(3156..4073)
                     /gene="deoC"
                     /locus_tag="PLL88_09815"
     CDS             complement(3156..4073)
                     /gene="deoC"
                     /locus_tag="PLL88_09815"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOJ01902.1"
                     /translation="MDANKEQIQTLAEIITKEILSVMREENNSAAKGQFCKVEVVDGL
                     QVKTCFDEFGEIISAGAERLTTTLGNIPDNLEMAGKIDHTLLKPEATREEISQLCFEA
                     RKFNFASVCVNPTHAKLCKSLLRGSSVKVCTVIGFPLGATTPEVKVFETRQAIEDGAE
                     EVDMVINIGGLKDKNYELVARDICGVVKEAHAHDALVKVILETALLTDEEKKIACLLV
                     KEAGAEYVKTSTGFSRGGATVEDVALMRRTVGPLMGVKAAGGVRTREDFEKMVSAGAT
                     RIGASAGVKIVKGDSEPQQAASAVPAKSY"
     gene            complement(4077..4631)
                     /gene="rpiB"
                     /locus_tag="PLL88_09820"
     CDS             complement(4077..4631)
                     /gene="rpiB"
                     /locus_tag="PLL88_09820"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOJ01903.1"
                     /translation="MDYISDSEIQTIVKRVLDKYTQYDNTPQSEKPTWVVAIGSDHGG
                     FTMKQSLLPYLEELGCRVIDCGPQTADAVDYPDFALKVAELVSTGNACRGIMIDGAGI
                     GSCMVANKVPGVRAAMCYDFATASNSREHNNANYLTLGAGLIGINQAKLITKTFLTTE
                     FGGGRHEKRVNKIMEIESRFLKKG"
     gene            4734..5480
                     /locus_tag="PLL88_09825"
     CDS             4734..5480
                     /locus_tag="PLL88_09825"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ01904.1"
                     /translation="MSRAIDPSNIIPKYYQLANILREQIENSTWKANETIPSERHLEQ
                     QYNLSRPTIRQAIDLLITQGFLYRIHGKGTFVSPPKLQKGVYELTSFSEDMRNRGLKP
                     GQIFLEFGAVAPPKKIRARLEITDPDQKVLRIKRIRTGNGEPIGIQDSYLSLPEGKTI
                     SKQELEERGSIYVILQEKFGIYPAEADETLEVTLASEEEANLLYISAGSPLLLNERIL
                     YSQDRKAIEFVSILYRGDRYKYYMRLTKKV"
     gene            complement(5499..6071)
                     /gene="lepB"
                     /locus_tag="PLL88_09830"
     CDS             complement(5499..6071)
                     /gene="lepB"
                     /locus_tag="PLL88_09830"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOJ01905.1"
                     /translation="MQNLPNSGKSQNISPLKRVSGVFLEILQTILIAAALYFVVDVII
                     GREEVFNVSMEPTVVQGEVIFVNKLAYRLDEADRGDIVTFHAPLDPQVNYIKRVIGLP
                     GDEVKISAGEVYINGKKIDEPYLNNVPMDENTWLVSEGMYFVMGDNRQNSIDSRTWGF
                     VPEGNLVGKALAVYWPITHIRLLKHVDIYP"
     gene            complement(6092..>6849)
                     /locus_tag="PLL88_09835"
     CDS             complement(6092..>6849)
                     /locus_tag="PLL88_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOJ01906.1"
                     /translation="VGKSSLLNKLAGEKRAIVSPIAGTTRDAIDTYITFHEIPITLID
                     TAGIRRRGKIDRGVEKYSVIRSMRAIERCDVALLLIDASQEVSAQDIHIAGYILDQWK
                     SAIVVVNKWDLVEKDTNTMDEYTQKIRHLLNFMDYVPVIFISALTGQRVNKVLPLALQ
                     IQEERVVHIGTGVLNRILMKAQDMHSPTSKTGRLLRIYYGTQVRADPPTFMLYVNDPE
                     LMHFTYQRFLENQLRAVYPFNGTPLRFVLKKRS"
BASE COUNT         1682 a         1914 c         1528 g         1725 t
ORIGIN      
        1 catttcaata tataaggatg ccatgccttc agttggtaga tcaccctggg tgaccgtgcc
       61 gacaaaggct gcatattgcg cttgcatgcg atctagcaag cagtaacatc gcaattgaaa
      121 tttatctgcc actcaaatct ccatttcttg cagccatctg tgcatggtgc atggcgatgc
      181 cgatgggggt tacaaacagc ggcttttccg gtgaatgggt agctatgccc gtataatcct
      241 gcaccacctc tgccatccct ggaaaacacg cggctcctcc aaccagggta atgctggtga
      301 tctttctgcc atcaagctgc cgggttatga tattaccaac cttttccatc accggtcgca
      361 ccaccgggaa cagcattttc tgcatggctg cattcttttt caattcttcc gcttcttcaa
      421 aactgatatt ccttgcaccg gcgatcacca aagaaaagtg tgttccaccg gttgcctcat
      481 ccgttgagaa gattactttg ccattttcca ccagcgcgat accggttgtt ccaccgccaa
      541 catctacaat ggcaccattt tggataccca gcagattatt ggcagcggtc ggctcatcca
      601 ccaatcccac acagttcaaa cctgcggctt ccaccacatt ggctgtggcg cgtacttctg
      661 cctgcggaac acccgggggg aaggaactgg ctgcatctgt taatgttcta ccaacgcgcg
      721 cttccaggcg ttgcttcatc tctttcaggc ggtcaactgc gccaaaaaaa tcaaccacca
      781 atccatcttt taccacctgg gcaaactggt actcgccagc taccggctgc aagttcttat
      841 caagcacgac caacactagg taagccgtac ccagatcagc gcctacataa agaggttcat
      901 catcaacagg cgctggcgag gtattgtgta tctggctcgg cagaatcgct tgttctgcgc
      961 gcgtcaggta ttctgtgaga tcaaactcac ctactttgcc cagcacagtg atctactctg
     1021 acgtgcactt atttcgcagt agtagagcga ccgctgatac tgcctttttc cttttgcggc
     1081 aggatcattt cgacatcgtt atgaggtcga ggaatgacgt gcacagagac caattcgccc
     1141 acacgctttg cagcagcggc gccggcatct gtggcagctt tcaccgcgcc aacgtctccg
     1201 cgcaccataa cggttacata accaccaccg acatactcgg agccgatgag ggttacgttt
     1261 gcggctttga ccatcgcatc tgcggcttca attgaaccga ccagaccttt ggtctccacc
     1321 atgcctaacg cgattaattc aggatttact gccatgactt cttcctttct tttttattaa
     1381 tttttcgcaa gcgtttcaag catcacctgt ctgacgatgc tttccaactc ctgctggttg
     1441 ggttccgaga atgccccaga cccaaccatg gctgcttcag gcggaggtgt agtttcatat
     1501 gccagccgct tgatattgaa caggtgatgg acggtaatat tatctccagt gacagaaccg
     1561 ccttcgcctc ctgctcccaa tgtaagtgag ggcatcacgc ccgtggtcaa cccgaccgca
     1621 cccagggtgc ccatggtatt gaccaaaaca cggaagacgg gtttttcaag cccaaatgcc
     1681 atgatcacat tttgatcggt ggcatgcaaa acaagggaat gtcctcttcc gccgaaatga
     1741 atcaattcaa tgcagcgttc acaaccatct tcccaaccct cttcctcaaa ccaacccaat
     1801 accgtggtca atttttcacg agaaagcggt tcttgcgcac ccacccgatt taagcgcacc
     1861 acgataatgc gcgcatccgc aggtacttcg aaaccaccca actgcgccag gaattgggga
     1921 gattttccaa ccgtggcgac attcatggtg ccatccgggt taaagaggat gcggcgcagg
     1981 atatctgctg ttcgttcatc ggcaaagtaa gctccattcg cctgcattcg ttgagccaat
     2041 tgtgccgcag caggcttatc gaccaccacg ctctgttctg aggcgcaaat gagagaataa
     2101 tcgaaagact tgcttgagac gatatagcgt gccgcttttt ccagatctgc gctgcggtcg
     2161 acataaaccg gaacgttccc cggtcccaca ccataggcgg gttttccaac cgaatgagcg
     2221 gctttgacca ttccctctcc gcctgtcgcc aggattacgc gaacgcgctt gtgtttcatt
     2281 aattcctgcg tgccaccaag agatatggtg cgcatgcagt tgatcaagcc aagcggggcg
     2341 ccagcattct ccgccgcctg ttgtagagta cgggcggctt cggcactgca gttcactgcc
     2401 gcaggatgtg gtgcgacgat gatcgcatct ctggctttta ccgccgagag cgctttcgcg
     2461 aacacggtag aagtaggatt cgtgctggga gtcaaagctg ccactacacc catgggccag
     2521 gcgatctcat ataaacgttt ttcgggatca tgcttgatca cgccaaccgt tttgatatcc
     2581 ttgatgctgt cccagagaac gcgcgaagcc agttcattct tcagtttttt gtgagcggca
     2641 acaccatatc cggtctcttc acttgccata cgtcccaggc gatcagaagc ctgatagccg
     2701 gcttctgcca tggctgcgca gacccgatca acctgttcct ggctggcttt accccacacc
     2761 agttgggctt cgtaagcctg cgctgccaga tcacgcgctt cctgaatgga tagtaaatct
     2821 ttgtcaatcg gcatcgttca tcccttttca gcttttccga aaaaccactt tcccatccac
     2881 ttccagtgaa tcaataacag ccataatgac cgcatccaca ggtcggttct tggttatctc
     2941 ggtctggcgg gcgctggaac catgcgccgt cagaaccaaa tcgcccacac cggcgtccac
     3001 gctatcaatc gcaacgtatg gctttccggc aggcgcaccc gtttcgtctg tctgacggca
     3061 aagaagcaac ttctgtcccg tcagggatgg atcttttatc gtcgagactg tcgtgccaat
     3121 aacccgggca atcagcattg ttcactcctt tccgttcagt aactcttggc aggtacagca
     3181 gaggctgcct gttgaggttc ggaatcacct ttcacaatct tcacccctgc gctcgctcca
     3241 atacgggtgg ctccggcgct gaccatcttt tcaaaatctt cccgcgtgcg cacgccgcca
     3301 gccgctttta cgcccatgag gggacctacc gtgcggcgca tcaacgccac atcttccacc
     3361 gtcgccccac ctctggaaaa accggttgac gtcttgacgt actcggcccc ggcctctttc
     3421 accaacaagc aggctatctt cttttcttca tcggtcaaca gtgcggtttc cagaataacc
     3481 ttcaccaaag cgtcatgggc atgggcttct ttgacaacac cgcatatatc gcgcgccacc
     3541 aactcatagt tcttgtcttt caaaccgccg atattaatga ccatatccac ttcttcagcg
     3601 ccatcttcaa tagcctggcg cgtctcaaac accttcactt caggggtggt agctcccaga
     3661 ggaaaaccga tcacggtaca aacttttacg ctgctaccgc gcagcaaact cttacacaac
     3721 ttggcatgcg tggggttgac acatacagat gcgaagttga acttgcgcgc ttcaaagcaa
     3781 agctgcgaga tctcttcgcg ggttgcttcg ggcttgagca aggtatgatc aatcttgccc
     3841 gccatttcaa gattatcagg aatatttcct aaggtagtgg tcaagcgttc tgcgccggca
     3901 ctgatgatct caccaaactc atcaaagcag gttttcacct gcagcccatc caccacctct
     3961 accttgcaga attgtccttt ggctgcagaa ttgttctctt ctcgcatgac gctgagaatc
     4021 tctttggtga tgatttctgc caatgtttga atttgttctt tattcgcgtc cataatctaa
     4081 ccctttttca aaaaccgact ttcaatttcc atgatcttgt tcacgcgttt ttcatggcga
     4141 ccaccaccga actcggtggt caggaaggtt tttgtaataa gcttagcctg gttgatacca
     4201 atcaatccgg ctcccaaggt aaggtaattg gcattattat gttcccggct gttagaagca
     4261 gtggcgaaat cgtagcacat agctgcccgc acaccgggta ctttattggc taccatgcaa
     4321 ctgccaatac ccgccccatc gatcatgatg ccgcggcagg cattgcccgt gctgacaagt
     4381 tcagcaactt tcaaagcaaa atccgggtaa tccactgcat cagccgtttg aggaccacaa
     4441 tcgatcacac ggcatcccaa ttcctcaaga taaggaagaa gcgattgctt catggtgaaa
     4501 cccccatgat cagacccaat ggctaccacc caggtaggct tttctgactg tggagtatta
     4561 tcatattggg tatatttgtc caaaaccctt tttacgatgg tctgaatctc agaatctgat
     4621 atataatcca tcttgcatcc tgtggttgac aaatcacttc gttttctata tgatgtatag
     4681 tggtaatgac cacattacat cataatgact atagtataac aggaatatga aatatgtcaa
     4741 gagccataga cccatccaac atcattccaa agtattacca gttggcaaac attctgcgcg
     4801 aacaaataga gaacagcaca tggaaagcga acgagaccat cccatcagag cggcatctgg
     4861 aacagcagta taaccttagc cgcccaacca tccgtcaggc tattgacctg ctcatcacgc
     4921 agggttttct ctatcgcatc cacggcaaag gcacgttcgt ttctccaccc aagctgcaga
     4981 aaggcgttta tgagctcacc agtttcagcg aagatatgcg caaccgtggg ttgaaacccg
     5041 ggcagatatt tctggagttt ggagcagtgg ctcctccaaa gaagatcaga gcccggctgg
     5101 aaatcaccga tcctgatcaa aaagtcctgc gcatcaaacg catccgcacc ggtaatggag
     5161 aacccattgg gatccaggat tcgtacctga gtttgccgga agggaagacc atctccaaac
     5221 aagaactgga agaacgcggt tccatctacg tcatcctaca ggaaaagttc ggcatctacc
     5281 ccgctgaggc ggatgaaacc cttgaggtga ccctggcttc ggaagaagaa gcgaacttgc
     5341 tctatatttc tgccggcagt ccactgctat taaatgaaag aattctgtat tcgcaggatc
     5401 ggaaagccat tgaattcgtc agcatcctgt accgcggcga tcgttacaag tactacatgc
     5461 gcctgacaaa aaaggtgtag agcgctggga tcgattttct acgggtaaat atcgacatgc
     5521 tttagaaggc gaatgtgagt gattggccaa taaaccgcca gggcttttcc aaccagattc
     5581 ccttctggca cgaaacccca ggttctggaa tcaatcgaat tctgccgatt atcacccatc
     5641 acaaaataca tgccttctga aaccagccag gtgttctcgt ccatgggaac gttattcaaa
     5701 tacggctcat cgatcttttt tccattgatg tacacctcac ccgcgctgat cttcacctca
     5761 tcccccggca aaccgatgac gcgcttgata taattcactt gcgggtcaag cggggcatgg
     5821 aacgtgacaa tatctccccg atccgcctca tccaaacgat acgccagttt attcacgaag
     5881 atcacctcac cctgcacaac ggtgggctcc atgctgacat tgaacacttc ttctcgacca
     5941 ataatcacat ccaccacaaa atacagcgca gcagcaatga gaatggtctg taaaatctcc
     6001 aagaaaacac cagagacgcg tttcaaagga gaaatgtttt gggattttcc agagttgggt
     6061 agattctgca tagaaaacgc tccgaatttt tttacgacct ttttttcaac acaaagcgaa
     6121 gcggggtacc attaaagggg taaaccgcac gaagttggtt ttcgagaaag cgctgatagg
     6181 tgaaatgcat gagttccgga tcattcacat acagcataaa cgttggtgga tccgcccgca
     6241 cttgcgtgcc atagtaaatg cgcagcaatc ttccggtctt tgaagtcggg gaatgcatat
     6301 cttgagcttt catcagaatt cgattcaaca cccctgtacc gatgtgcacc acacgttctt
     6361 cctggatttg taaagccagg gggagcactt tgttcacccg ctgaccggta agcgcagaaa
     6421 tgaaaatgac tggcacataa tccatgaaat tgagcaaatg gcggatcttc tgtgtatact
     6481 cgtccatagt gttcgtgtct ttttcgacca gatcccattt attgacaacc acgatcgcgc
     6541 ttttccattg atccagaatg tagccggcaa tatgaatatc ctgggcagac acttcctggc
     6601 tggcgtcaat caacaataac gccacatcac agcgttcgat agcccgcatc gaacggatta
     6661 ccgaatattt ttcaacgcca cgatcaattt ttcctcttct gcggatacca gccgtatcga
     6721 tcagtgtgat aggaatttcg tggaacgtta tataggtgtc aatggcatca cgtgtcgttc
     6781 cggcaatggg gctgacgata gcgcgttttt ctcccgccaa tttgttcaac aaactggatt
     6841 tcccgacat
//
LOCUS       DAOSDS010000104         6756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4114_length_6756_cov_4.243397, whole genome shotgun sequence.
ACCESSION   DAOSDS010000104 DAOSDS010000000
VERSION     DAOSDS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4114_length_6756_cov_4.243397"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1534)
                     /locus_tag="PLL88_09840"
     CDS             complement(170..1534)
                     /locus_tag="PLL88_09840"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOJ01907.1"
                     /translation="MKKIIETYDQALDFIYSKVDFSYTKQQRYSEEVFNLDRMLSFMK
                     PLGDPQNTFRAVHVAGTKGKGSISAMIASALQTAGYRTGFYSSPHMIDYTERIQIDSK
                     PIPKEDFVRIINRMYPLIQKTPGLTTFEITTAVAFEYFKDMQIEFGVIEVGLGGRLDA
                     TNIINPEVSIIASISLDHTGILGNTVEAIAAEKAGIIKHGIPVVISDQIPSVYDVFHK
                     ITQEKSAPFINVREVLDYRDRKSDLSHQTFTVHFPERITNGSREKNGMLDITIPLLGK
                     HQMENAGAAFCALKVLQEKGYKISDRSIQSGFCNVSWPGRFEILRKEPYLIIDAAHNL
                     DSSHRLVETIQELFPAKKITLIFGASEDKDIAGMLDNFSKVARLVILTQSTHPRAAKP
                     DVLQKSMRAAPCDTIMIEDVQRALEYALEHAQKDDVILVSGSVFIAAAIREIWFSRHT
                     EITV"
     gene            1600..1884
                     /locus_tag="PLL88_09845"
     CDS             1600..1884
                     /locus_tag="PLL88_09845"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOJ01908.1"
                     /translation="MHVGVLTFRILLYNSHSLKAKRSQIKPLLSYLQKRFNVSAAEIE
                     LLDHWDQSVLACAMIGNDRRFLESSLASVFAQMENRFRDVQFLGSKIEIW"
     gene            1892..2683
                     /locus_tag="PLL88_09850"
     CDS             1892..2683
                     /locus_tag="PLL88_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ01909.1"
                     /translation="MDLFLKEKTALITGSSKGLGFATARQLLMEGATVIINGRNEDSL
                     SQAKLELEKIPGARVFAVAGDVAEADFPTRLVQEIQAHFDGLDILITNAGGPPAGAMD
                     DFSIEEWEKAINTTFLSHVRLIQAAKPLLMKSPSPSILTITSAAVKQPMPNMILSNSI
                     RAATVGLTKSLANEFGPLGIRVNSILPGWTDTERVQSLMQIRATANRSSIEEEYRKQA
                     AESPLRRIGKPEEFARVATFLVSPAASYVHGVMLVVDGGTSKGLL"
     gene            2684..4525
                     /locus_tag="PLL88_09855"
     CDS             2684..4525
                     /locus_tag="PLL88_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOJ01910.1"
                     /translation="MKRTPISAEEISHFLDRTLFNIEKPGRYVGGEFNQVIKDWASIS
                     VKAVLAFPDVYEIGFSNLGLNILYDEINACSVFLAERAFSVWDDMELSMRNNDIPLFS
                     LETKHPVRDFDVLGISLPYETLYTNALNLIDLSAIPVLRKNRQDRDPLVLAGGHATFN
                     PAPMNAFIDAFVIGDGEQIVLKILHIVNEGLSREKTLEKISALKSVYVPEFYDIQRYF
                     AVGGQQSLKPIKKNMIATLPTPPEKPLVPNIETTHDRIAIEVMRGCSHGCRFCQAGFI
                     ARPVRERPLQDILASLRSSIQQTGYDEISLLSLSISDYSAIQDLIMSISKEFKGLNVN
                     LSLPSLRIESFSEDLMHTMNQRKGNFTLAPESATESMRRSINKPISDDDLLETATLIF
                     QKGWTSIKLYFLIGLPGETMDDVQHIVELCRKVKTIGKRLIGGRARISVSINTFIPKS
                     HTAFQWVAMDSIANVGQKHRLLRDGFRNTGIILNFPSMESSLVEGWLSRADEKVAMVI
                     YAAWQKGAKFDAWQDRLDVEIWKSAFLENGLDPFIYSHRTRTIAEQLPWDFIDTGISK
                     AYLIEEYRRSMEGSLTSDCRTQCHACGIQSNYAVLCRDLIGKSYGNP"
     gene            4512..5186
                     /locus_tag="PLL88_09860"
     CDS             4512..5186
                     /locus_tag="PLL88_09860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOJ01911.1"
                     /translation="MEIHEERIRITYSKLAPMRFTSILDVQTIWERTFRRSGVILSYS
                     KGFHPQAKIQQASPLPLGFCGEQEIIDVWIDAANKQSLTKETINPFLPNGMQIQQVET
                     VDLHAPTLQQNVVFSDYSACFLQPVSEKELVSLVEKIQRSKNIQYTRHNGKQYDLKPL
                     IRSAHVHKDTVQGAMLLLSLSTSPSATGRPDHLLHYFHYDPSLILITRKMIHFLHEQI
                     CNSSVH"
     gene            5155..5781
                     /locus_tag="PLL88_09865"
     CDS             5155..5781
                     /locus_tag="PLL88_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HOJ01912.1"
                     /translation="MNRYVILQCTNRLCNFRFPAEVGNYGIYCPLCGNELTVREYRVD
                     NGYAQFNKKDTALECVLDNVRSSYNVGSIFRSASAFSVARLFLCGFTPTPDQAKVRKT
                     SLGAEKFVLWSHEPNALQLIQQKKEQGYQIIAIEASLSAQSLLDWSIKSGEQKVLLIF
                     GNEIFGIDPEIRAFSDALLQIPITGDKKSLNVAAAFSISVFYFSSCLS"
     gene            complement(5776..6318)
                     /locus_tag="PLL88_09870"
     CDS             complement(5776..6318)
                     /locus_tag="PLL88_09870"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HOJ01913.1"
                     /translation="MPLVWIITGKQNAGKSVLMMDIIAEVKQRRYPLCGLVSPGVYQD
                     GTRIAIQVLDVGSGERTILAELKPGWDPENPTKKWKMNEEAVQWGAARLGSMDPAGKV
                     FFLDEIGIYEILEKTGWQKGLEILQEKTYYRAFISVRKEVVTEIIKICENADIFFEEI
                     DLDAIQTKRQAIVQKMVQDL"
     gene            complement(6359..>6756)
                     /locus_tag="PLL88_09875"
     CDS             complement(6359..>6756)
                     /locus_tag="PLL88_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HOJ01914.1"
                     /translation="DVLEREGYIRPSKRIEDESGYPPKKFFEITEKGNEIYRSWVHEP
                     VKHGREIRQVFLSKLFFTQTENKQLAHQLILDQLSECNRWITNSKPIGEGEGSEFSAI
                     VESFRSMQIKSYVAWLKELGKEVEKTLSN"
BASE COUNT         1741 a         1647 c         1448 g         1920 t
ORIGIN      
        1 acaaagatag gcgaaatcat cttggggaag atgatgaaat cttttagtat caaagccttg
       61 gaatgaatga atccgctctc gtcttgtacc tggtttggga tttcatttag ctcatcggat
      121 ctctgcaata aagctgctga aatattttca tgtttatctg aattgttcat taaacagtta
      181 tctccgtatg cctactaaac catatctccc tgattgcagc agctatgaag acgctgccgc
      241 tgacgagaat gacatcatct ttttgggcat gctcgagggc atattccaga gcgcgttgca
      301 catcctcaat cataattgta tcgcaaggcg ctgctcgcat gcttttttgt agcacgtctg
      361 gtttggctgc ccgaggatgg gttgattgcg tgaggatgac caggcgcgcc actttcgaaa
      421 aattatccag catgcctgca atatctttgt cttccgatgc tccaaaaatc agggtgatct
      481 ttttagcggg gaacagttct tggatcgttt ctaccaatcg atgcgaagaa tccagattgt
      541 gagctgcatc aatgatcaag taaggctctt ttcgcaagat ttcgaagcgc cccggccatg
      601 aaacattgca aaatcccgat tggattgaac ggtcagagat tttgtagcct ttttcctgca
      661 gcacctttaa cgcgcaaaaa gcggctccgg cgttctccat ctgatgtttt cccaaaagcg
      721 gaatggttat gtctagcatt ccattctttt cccgagaccc attggtgatt ctttcaggaa
      781 aatgaacggt gaacgtttga tgagacagat cacttttccg atcacgatag tccagtacct
      841 ctctcacatt gatgaatggg gcactttttt cctgggtgat cttgtgaaac acgtcataaa
      901 cggaaggaat ctgatcagaa atcaccaccg ggatgccgtg tttgatgatc ccggcttttt
      961 ccgctgcaat cgcttcaaca gtattcccga gaattccggt gtgatcgaga gaaatcgatg
     1021 cgatgattga tacttccgga ttgatgatat tggttgcatc caacctaccc cccaagccaa
     1081 cttcgataac cccaaattct atctgcatat ccttgaaata ttcaaatgcc accgccgtgg
     1141 tgatctcgaa tgttgtcaac ccgggagtct tttggatcag gggatacatg cggttaatga
     1201 tacggacaaa gtcctctttg ggaatcggct tcgaatcgat ttggatccgt tccgtataat
     1261 cgatcatgtg aggggatgaa tagaatccgg ttctgtatcc tgccgtctgc aatgccgatg
     1321 cgatcatggc agaaattgat ccttttcctt tcgtgcccgc aacatgaact gctcggaaag
     1381 tgttttgtgg atctcccaat ggcttcatga atgagagcat ccgatccaga ttgaacactt
     1441 cttctgaata tcgctgttgt ttggtgtaac tgaaatctac cttggaataa ataaaatcca
     1501 gcgcttggtc gtacgtttca atgatcttct tcattcagaa tcattacctc gaatgacaat
     1561 cttactatta atccatttct aagactataa tcttttttaa tgcatgtagg tgtattaact
     1621 ttccgcatct tgctatataa ttcccactcg cttaaagcga agcgcagcca gatcaaacca
     1681 ttgttgagct atttgcaaaa aagattcaac gtctctgcgg cggagatcga gctgctggat
     1741 cattgggatc agagtgtgct ggcatgtgca atgatcggga atgatcgccg tttcttggaa
     1801 agcagcctgg catcggtttt cgctcagatg gaaaatcgtt tccgggatgt acaattttta
     1861 gggtcgaaaa tagagatttg gtaggtgaag catggatctt tttctcaaag aaaaaactgc
     1921 gttgataacc ggttccagca agggattagg gtttgcaact gcccgccagt tgctgatgga
     1981 gggtgccacc gtcatcatca atggtcgtaa cgaggactcc ctttcacaag caaaattgga
     2041 attggaaaaa atccctggcg cccgcgtttt tgcagttgcc ggcgatgttg cagaagcgga
     2101 ttttccaact cgtctcgtac aggagattca agctcacttt gatgggcttg atattttgat
     2161 caccaatgca ggtggcccgc cggctggtgc gatggatgat ttctcgatcg aagagtggga
     2221 aaaggcaatc aatactactt ttttaagcca cgtccgtttg atccaggctg ccaagccact
     2281 tcttatgaag tcaccatctc ccagcatttt aactatcact tctgctgccg taaaacaacc
     2341 aatgcccaac atgatccttt cgaattccat ccgggctgct acggttggtc ttacaaaatc
     2401 gctagccaac gaatttggac cgctcgggat acgggtaaac tcgattctcc ccggttggac
     2461 ggatacagaa cgcgttcaga gcctgatgca aatccgcgcc acggcaaacc gctcatcgat
     2521 tgaagaagaa tatcgcaaac aggctgccga atcccccttg cgccgaattg gcaaacctga
     2581 agagttcgct cgtgtggcta cttttctcgt ttcacccgct gcttcctatg ttcatggagt
     2641 gatgctggtt gttgatggtg gcacaagcaa agggctgttg taattgaaac gaacgcccat
     2701 ctccgctgaa gagatcagtc attttttaga tcgcacgctc tttaatattg aaaaacccgg
     2761 gcgttatgtg gggggtgaat ttaaccaggt gataaaagat tgggcttcca tctctgtaaa
     2821 agccgttctt gccttcccgg atgtttatga aattggattt tctaatcttg ggttgaatat
     2881 actctacgat gaaatcaatg cttgctctgt ttttttggct gagagagcat tttctgtttg
     2941 ggatgatatg gaactgtcta tgcgcaataa cgacatccca ttattctccc ttgagaccaa
     3001 acaccccgta cgtgatttcg acgtgcttgg aatttcactg ccgtacgaaa ctttatatac
     3061 caacgcgttg aatctcatcg atctctccgc cattcccgtt ctgcgcaaga accgccaaga
     3121 tcgtgaccca ctggttctcg ctggaggtca tgccactttc aacccggcac ccatgaacgc
     3181 tttcatcgac gccttcgtca tcggcgatgg cgagcagatc gttctcaaga tcctgcatat
     3241 cgtcaatgaa ggtctttcac gagaaaagac ccttgagaag atcagcgcac tgaaaagcgt
     3301 ctacgttcct gagttctatg atatccagcg gtatttcgca gtaggtggtc aacaatcgct
     3361 caaaccgatc aaaaagaaca tgatcgccac ccttcccacc ccaccggaaa aaccgctggt
     3421 gccgaatatc gaaaccaccc atgaccggat cgctattgag gtcatgcgcg gttgttcgca
     3481 tggttgccgc ttttgtcagg cggggtttat cgcccgcccc gtgcgagaac gacccctcca
     3541 ggacatcctt gcatcactcc gctcgtccat tcagcagaca ggttacgatg aaatatcttt
     3601 gctctcttta agtatcagcg attattctgc catccaagat ctcatcatga gcatttcaaa
     3661 ggaattcaaa ggactgaatg tcaatttgtc ccttccatca ttgagaatcg aatcgttttc
     3721 tgaagattta atgcacacca tgaaccagcg gaaaggcaat ttcaccctgg cgcctgagtc
     3781 agccaccgaa tccatgcgcc gttcgatcaa caaacccatt tcggatgacg atttgctgga
     3841 aactgcgacc ttgatctttc aaaaagggtg gacttccata aaactatact tcctgatcgg
     3901 attacccggg gaaaccatgg atgatgttca acacatcgtg gagctttgcc gtaaagtgaa
     3961 gactattggt aagcggttga tcggcggtcg cgccagaatt tctgtgagca tcaacacatt
     4021 tataccgaaa tcacacaccg cttttcaatg ggtagccatg gattcaatcg cgaatgtggg
     4081 tcagaaacat cgcttattga gggatggttt tcggaacact ggcatcatcc ttaactttcc
     4141 aagcatggaa tcatcattag tagaaggttg gctgtcacgc gccgacgaaa aggtcgcgat
     4201 ggtgatctat gcagcctggc aaaaaggcgc aaaatttgat gcctggcaag atcgccttga
     4261 tgtggaaatt tggaaatctg cttttcttga aaacgggctt gatccattca tctacagcca
     4321 ccgaacgcgc acaatagcag agcaattgcc ctgggatttc atcgataccg gcatatccaa
     4381 agcatatctg atagaagagt atcgccgatc gatggaaggt tcgctcacca gcgattgccg
     4441 tacccaatgc catgcttgcg gaattcaatc aaactatgcc gtcctttgcc gtgatctgat
     4501 aggaaaaagc tatggaaatc catgaagaaa gaatacggat cacctactca aaactggcgc
     4561 cgatgcgctt cacatcgatt ttggatgtgc aaacgatttg ggaaaggacc tttcgccgct
     4621 ccggagttat cctttcttac tcgaaaggat ttcacccaca ggcaaagatc cagcaagcgt
     4681 ctcctttacc gctgggtttc tgtggcgaac aggaaattat tgatgtctgg atcgatgcag
     4741 caaacaagca atcgctgaca aaagaaacga tcaatccttt tcttccaaat ggcatgcaga
     4801 ttcaacaagt ggagaccgtt gacctgcatg cgcctacgtt acaacaaaac gtggtctttt
     4861 cagactattc tgcttgtttt ttgcagcctg tttccgaaaa agagcttgtt tcgcttgttg
     4921 aaaagattca acgatcaaaa aatattcaat acacgaggca taatgggaaa cagtatgacc
     4981 tgaaaccttt gatccgttcc gcacatgttc acaaggacac cgtacaaggt gctatgcttt
     5041 tactgtccct ttccacatct cccagcgcga ccggcagacc tgatcacctt cttcactatt
     5101 tccactatga cccttcgctt atcctgatca ccagaaaaat gatccatttt cttcatgaac
     5161 agatatgtaa ttcttcagtg cactaaccgg ttatgcaact ttcgttttcc agcagaagtg
     5221 ggaaactatg gcatctattg cccgctttgt ggaaatgaat taaccgtccg ggaatatagg
     5281 gtggataatg gctatgcgca attcaataaa aaggatacag ccctggaatg cgttcttgac
     5341 aatgtgcgct ccagctataa cgttggttcc atctttcgct cagccagtgc tttttctgta
     5401 gcacgcctct ttctgtgcgg ctttaccccc accccagacc aagcaaaagt cagaaaaacc
     5461 agtctcggcg cggaaaaatt tgttctgtgg agccacgaac cgaacgcctt gcagctgatt
     5521 caacaaaaaa aagaacaggg atatcaaatt attgctatcg aagcctccct ctccgcccaa
     5581 tctctgctgg attggtctat aaaatcaggt gagcaaaagg tattattgat ctttgggaac
     5641 gagatctttg gcatcgatcc ggaaatcaga gctttcagcg atgcgctgct ccaaattcct
     5701 atcaccggtg acaaaaaatc gttgaatgtt gctgccgctt tttctatttc cgtgttttat
     5761 ttttctagct gcctatcata aatcctgcac cattttttgc acgattgctt gccttttggt
     5821 ctgaatggca tccagatcaa tttcctcaaa gaaaatatca gcattttcgc atatcttaat
     5881 tatttccgtt acaacttctt tccgtaccga gatgaaggca cggtaataag ttttctcctg
     5941 tagaatttcc agcccttttt gccaccctgt cttttccaga atttcataaa tcccaatttc
     6001 gtccaggaaa aacactttcc ccgctggatc catgctaccc aagcgggctg ccccccattg
     6061 aaccgcctct tcattcattt tccatttctt tgttggattt tcaggatccc aaccaggttt
     6121 caattctgcc aggatcgtcc gctcccctga tccaacatcg agcacttgta tcgcgatccg
     6181 tgtaccatcc tgataaactc cgggagaaac caatccgcac aatggatatc ttctctgttt
     6241 tacttctgca atgatatcca tcatcagcac agattttcca gcattctgtt tcccggtgat
     6301 tatccaaacc aacggcacaa tttttctcct acacgaatgg catgatcttt gcaaatcctc
     6361 aattagagag agttttttct acttctttcc ccagttcttt cagccatgcg acgtagcttt
     6421 taatttgcat cgagcgaaag gattcgacga tcgcactgaa ttcagatccc tccccttccc
     6481 caattggctt tgaattcgtg atccaccgat tgcattcact aagctgatca aggatcaact
     6541 ggtgtgccaa ctgtttgttt tcggtttgtg taaaaaataa tttggaaagg aaaacctgtc
     6601 gtatttctct gccatgtttt accggttcat gcacccacga gcgatagatc tcgtttcctt
     6661 tttcagtaat ctcaaaaaat tttttcggcg gatagccaga ttcatcttca atgcgtttcg
     6721 agggtctgat atatccttct ctttcaagca catcca
//
LOCUS       DAOSDS010000105         6728 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4135_length_6728_cov_4.244418, whole genome shotgun sequence.
ACCESSION   DAOSDS010000105 DAOSDS010000000
VERSION     DAOSDS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4135_length_6728_cov_4.244418"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLL88_09880"
     CDS             complement(<1..998)
                     /locus_tag="PLL88_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01915.1"
                     /translation="MLQENENRLKPPKIIQTLVQGFNVVAAHPYVMLFPLLLDLLFWF
                     GPFYRIKELLSPALEEMLETLSASLTTADVATVLQTTRTTWDQLFSNFNLLTVLRTYP
                     IGIPSLLASKGYSANPLGNPLLIEVESSSEALLVIVLCVFLGLFLGALYYGLISRLLR
                     KSKEKEKSAHFFYSIGQTFVLFILIVLAILLLSFPVLCFLSSFSVFVPTIGTLPLMIL
                     GLILLWIFVPLVFSPHGIFTRRLSAFKAISLSTKFVRISSMATTLFLTLAVALSYGLD
                     LLWSTPAPDSWLYFLGILGHAFVSSGILAASFVYFRDGTIWMDEIIKLGPQKTTGT"
     gene            1095..1904
                     /locus_tag="PLL88_09885"
     CDS             1095..1904
                     /locus_tag="PLL88_09885"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01916.1"
                     /translation="MKNQSIPTDRLSIISSLIILNYALMPFIYSPRIPIQLSLFGILF
                     DLSIKYSDLMVISAAAFAAIGTYWLLFDHPLINKSNSIIHLILPTLIAGVMSIPLNVI
                     NIGPAWWAVFALESILIILTLIAEYYSIDPNHSLFALSKIILIPLAISLILLLSISAR
                     SAGYRLYIETAVLGVAFGAISVRLNALTHSGEKRAKTLSSTYLFIQILVVCHYLPLTS
                     IPFGLVLTGWATFLIGISLADDLEKNFSATLRNAAFYAIPFLIGAILFLFV"
     gene            complement(1941..2792)
                     /locus_tag="PLL88_09890"
     CDS             complement(1941..2792)
                     /locus_tag="PLL88_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ01917.1"
                     /translation="MIRERISENVYWFQSEVYAQVAAGAVIGTDWAVVIDTLPVQEET
                     LEIREFIEEHLKVPVRFVINTIYHADHTWGNCFFPGAGVIAHTLGRKLVKEKNIPALE
                     NAQQQGEEYLKTKIILPQITLDAGSMNMRVGKKSLKVFTTPGNSPDGISVFVREDRIL
                     FTGDAFLPLPVFIEGDIDQLIQTIESFKDLGLENIVQGHGDVILRGEIEGAIDSNLKY
                     LDELKKVVAVALKRKDPQKVLEETTVESCGKSRVLLGGLVEELHRRNLVHLYQEELKK
                     LGTDPVL"
     regulatory      2935..3040
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3109..5712
                     /locus_tag="PLL88_09895"
     CDS             3109..5712
                     /locus_tag="PLL88_09895"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOJ01918.1"
                     /translation="MKNYGYLEALKESILIFDGAMGTNLQALKLQAADFGGEQHAGCN
                     DVLCLTRPSAIEQIHRSFLEAGVDVIETNTFRSNRITLSEYGLEDKVFPINTQAARLA
                     KRLAKTYSTSGQPRFVAGSIGPSGKILSIDENRSASVPFPTLVEVFKEQALALLEGEV
                     DLLLLETQQDLLEVKAAILGIRKACQESDRYVPIQTQVSMDTHNHMLLGSSIDAVSAI
                     LEPLEIDVLGLNCSTGPDEMEPAIAFLGKNASLPISCLPNAGMPEMRDDEVIYPLSPE
                     HFAQQLAHFVAKHSVSAVGGCCGTTPEHLRLLVEKVHDYPAPHKQKSQGSFLSSPLHA
                     QAIRQSPPPFIIGERLNTQGSRNFKTIILAGDFAQAETLARQQVENGAHGLDVCTALT
                     ENDLEKQNMVTLIRRISHTLDAPLIIDTTNPQVMEAALQSTPGRCLLNSTNFESGETK
                     ARDTFNLARQYGAAVLVLTIDEHGMAKSTEEKLAIVERAYQMAVNEIQLSPQSLVFDP
                     LTFSLASGDEGTLNSAVVTLSAIHAIKQRYPNCHVSLGISNISYGFSKPARKILNSVF
                     LYHALQAGLDMAILNAADLVPYTEISADEKEATERLIFHNSAQALADFIAIFTEGKRN
                     HAEVESPPISTRMQPGEKVYRRITNRTRDGLEEDIVRYIQEKSRQQSPQHAALSLLNE
                     HLLPAMKEVGEQFSNGEIILPFVLQSSEIMRAATTKLEEYIQRQEQEEMGRVLLATVY
                     GDVHDIGKNLVRTILENNGYSVIDLGKQVPAEQIVETALDKNVTAIGLSALLVNTSQQ
                     MPLVVEKLRAQRSNIPLLIGGAAVNQTFAKRIEKDEQGAAYAGGVFYCKDAFDALAIL
                     GGLQQSRKGMQ"
     gene            5709..6470
                     /locus_tag="PLL88_09900"
     CDS             5709..6470
                     /locus_tag="PLL88_09900"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOJ01919.1"
                     /translation="MNSADPTPVLGAHIIPHIAVEELLPFLNRKALYRISWGAQKAQG
                     AQWEKIQKEYEGKLESMLSELRTNAWITPAAGYGHWMANSEGDTILLYDPQNSEKIIA
                     RFPLPRQQSGERLCLADYLPACSSGISTILSLQVVSMGHKASETANRFFSNGNYAEGY
                     FRHGLAVQFAEAAAEYVHALIRKELSLEKRQGRRYSWGYEPIPDLSQHRILFELIPAQ
                     AELDMDLTSACQLIPEQSTAAMVIHHKAARYFRIL"
     gene            6486..>6728
                     /locus_tag="PLL88_09905"
     CDS             6486..>6728
                     /locus_tag="PLL88_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOJ01920.1"
                     /translation="MNRDAFLKRLREKKPLVADGAMGTLLHDRGVGFESCFDELNLTN
                     PALVGSIHQEYIKAGAEVILTNTFGANRFKLERCGLE"
BASE COUNT         1838 a         1776 c         1629 g         1485 t
ORIGIN      
        1 tgcgtaccgg tcgttttctg cggacccagt tttatgatct catccatcca gatcgtacca
       61 tcgcggaaat agacgaaact ggctgccaag atacccgagg aaacaaaagc atgaccaaga
      121 atccccagaa aatacaacca actatcaggg gcaggcgtgg accacaacag gtcaagccca
      181 taactcaaag caacggcaag ggtaagaaag agggtggtcg ccattgatga aatgcgcaca
      241 aatttggtgc tcaatgaaat cgccttgaac gcgctcagac gccgggtgaa aattccatgc
      301 ggcgaaaaaa cgagaggaac aaatatccac aacaaaatca gacctaaaat catgagaggg
      361 agcgtgccta tggttggcac aaaaacggaa aatgaggaaa ggaagcacag caccgggaag
      421 gataacaaga gaatagccag cacgatcaaa ataaagagca caaaagtctg accaatggaa
      481 tagaagaaat gcgcgctctt ttctttttct ttcgacttgc gcaataagcg ggagatcaag
      541 ccataataga gagcgcccag gaaaagaccc aagaacacac agagaacgat caccaagagt
      601 gcttcagaag atgattctac ttcgatcaac aaagggttcc ccagcgggtt ggcactgtat
      661 cctttggagg caagcaaact aggaatgccg atgggatagg tgcgcagcac ggtcaacaaa
      721 ttgaagttag aaaacaactg atcccaggtg gtgcgggtgg tttgtaaaac cgttgcaaca
      781 tcagccgtgg tgagcgatgc agacaaggtt tccaacatct cctccagcgc aggacttaac
      841 agctctttaa tccggtagaa tggaccgaac cagaacaaaa gatccaacag gagcggaaag
      901 agcatgacgt aaggatgcgc agcaactaca ttaaatcctt gcacaagcgt ctggatgatc
      961 ttgggcggct ttaggcggtt ttcattttct tgtaacatac aatcctccat tgtaccatat
     1021 tgacaatgct tactcctgga cgaaccagcg ccgtggtaaa aaccagcaga agcggtgtac
     1081 aatcgagaag aaccatgaag aatcaatcca ttccaaccga tcgtttgagc atcatttctt
     1141 cgttgatcat cctaaactac gcattgatgc cattcattta ctcaccccgc attcccatcc
     1201 aactctcatt gtttggcatt ttatttgacc tctcgattaa atattccgat ttgatggtca
     1261 tctctgccgc ggcgttcgcc gcgatcggca cgtattggtt attgtttgac cacccgttga
     1321 tcaataaatc caacagcatc attcatctca tcttgcccac cctgatcgcg ggggtgatga
     1381 gcatcccctt gaatgtgatc aacatcggcc cagcctggtg ggcggtgttc gcgctggaaa
     1441 gcattctgat catcttgacc ctgatcgccg agtactacag tatcgatccg aaccattcgc
     1501 tcttcgcact gtccaagatc atcttgatcc cattggctat cagcttaatc ttgctcctat
     1561 ccatttcagc cagaagcgcc ggctaccggc tgtacatcga gacagcggta ttgggagttg
     1621 ccttcggcgc catatccgtc cgcctaaacg cattaacgca ctccggtgaa aaaagggcaa
     1681 agacgctgag ctccacctac ctgttcatcc agattctggt ggtctgccat tatctccctc
     1741 tgacctcaat tccgttcggg ttggtgttga caggttgggc aacgtttttg atcggcatct
     1801 ctctcgcaga cgatctcgag aaaaattttt ccgccacctt gcggaatgca gctttttatg
     1861 ccatcccatt tctgatcggt gccatactct ttttattcgt atgaccaaga gaaatagagc
     1921 aaccctggtg gtctgaatgc ttacagaacc ggatcggtgc ccaacttttt cagttcttct
     1981 tgatacaggt gaaccaggtt gcgccgatgc aattcttcga caagcccccc cagcagaaca
     2041 cggctcttgc cgcacgattc caccgttgtt tcctccaata ctttttgcgg gtcttttctt
     2101 ttgagggcta ccgccaccac ttttttcaat tcatccaggt atttcagatt cgaatcaata
     2161 gcgccttcga tctcgccgcg caagatcaca tcgccatgcc cttgaacgat gttctcgagc
     2221 cccagatctt taaaggattc tatggtttga atgagctgat cgatatcgcc ttcgatgaaa
     2281 acaggcagcg gcaagaaggc atctccggtg aagagaatgc gatcttcacg cacaaaaacg
     2341 gaaattccat cagggctgtt cccgggggta gtgaagacct tgaggctttt ctttcctacc
     2401 ctcatgttca tggagccagc atccaatgtg atctgcggaa ggatgatttt cgttttcaaa
     2461 tactcttcac cctgctgttg ggcattttcc agagcaggga tgttcttttc tttcaccagc
     2521 tttcttccca gcgtgtgggc gatgacacca gcacccggaa aaaaacaatt cccccaggta
     2581 tggtcagcgt gataaatggt attgataaca aagcgcaccg gcactttgag gtgttcttca
     2641 atgaattccc tgatctccaa agtttcttcc tgtaccggca aggtatcaat caccactgcc
     2701 cagtcagtgc caatgaccgc tccagccgcc acctgggcat atacttcact ctgaaaccag
     2761 tacacattct ctgaaattcg ttcacgtatc atgggaattc tctatattaa tgaaatatta
     2821 ctaaaaatca agaatagcat atatttttcg aaaagtcaaa attttagaac atgtatccta
     2881 tttcgcttac agattgacca tctttctgtc ttgtgataag ataagtgcca attaagctca
     2941 tccagagagg tggagggacc ggccctgtga aacctcggca accaatgttc ggtgccaatt
     3001 ccggcagaag ccgcccagcg gtgattctgg aagatgagag aaacggtttc aaaaacctct
     3061 cattccagag aggttttttc tttatcaaaa agaaagcgag actgtcattt gaaaaattac
     3121 ggttacctcg aagctctcaa agaatccatc ctcatctttg atggggcaat gggaaccaat
     3181 ctgcaagcat tgaagctcca ggctgccgat ttcggagggg agcaacacgc cggctgcaac
     3241 gatgttctct gcctgacccg tccgagcgcg atagaacaga tccatcgttc cttcctggag
     3301 gcaggtgtgg atgtcatcga aaccaacacg tttcgttcca accggatcac attaagcgaa
     3361 tatgggctgg aagataaggt atttccaatc aatacacaag ctgcgcgcct ggcaaaacgc
     3421 ctggcaaaaa catacagcac aagcggtcaa ccgcgatttg tggctggctc catcggccct
     3481 tccggaaaaa tcctttcgat cgacgaaaat cgatctgcgt ccgttccctt tccaacgctt
     3541 gtggaggtct tcaaagaaca ggcgttagcc ttactggaag gcgaggttga tctgctgctg
     3601 ttggaaacac agcaggatct gctggaggtg aaggcagcca tccttggcat tcgcaaagcc
     3661 tgtcaggaaa gcgacaggta tgtgcccatc caaacgcagg tgagcatgga cacgcacaac
     3721 catatgctat taggcagctc gatcgacgcg gtgtcggcga tcttggaacc gcttgaaatc
     3781 gacgtgctgg gtttgaactg ttccaccggt ccggacgaga tggagccagc tatcgcattc
     3841 ctaggaaaaa atgccagcct gcccatatct tgcctgccca acgcaggcat gcctgaaatg
     3901 agagacgatg aggtaattta ccccctgagc ccggaacatt tcgcccaaca actggcacac
     3961 ttcgtggcaa aacacagcgt gtcggcggta ggcggttgct gcggaaccac accggaacac
     4021 ctgcggttgc tcgtagaaaa agtacacgat tatcccgcac cgcataaaca aaaaagccag
     4081 gggagctttc tcagttcgcc cctgcacgcc caggcaatac gccagagccc tccgccattc
     4141 atcattggag agcggctgaa cacccagggt agccgaaatt tcaaaaccat catcctggca
     4201 ggcgatttcg cgcaagcgga aacgctggct cgacaacagg tggaaaacgg cgcgcatggg
     4261 ctcgatgtat gcaccgcctt gaccgaaaat gacctggaaa aacaaaacat ggtcacgctc
     4321 attcggcgca tcagccacac gctagacgcc ccactcatca tcgacaccac caacccacag
     4381 gtcatggaag cggcactcca gagcacgccg gggcgatgcc tgttaaactc gaccaacttc
     4441 gaaagcggag agacaaaggc gcgcgacaca tttaacctgg cacgccaata cggggcggcg
     4501 gtgcttgttc tcaccataga cgaacatgga atggcaaaat ccaccgaaga gaaactagcc
     4561 atcgtcgagc gcgcctacca gatggcggtg aacgagatac aactttcacc ccaatctctg
     4621 gttttcgatc cattgacctt ttcgctggca agtggagatg agggcacgct gaacagcgcc
     4681 gtggtaaccc tttcagccat acacgccatc aagcaaagat accccaactg ccatgtatcg
     4741 ctcggcatca gcaacatctc ctacgggttc tctaagccag cccgcaagat cttgaacagc
     4801 gtgtttctgt accacgctct acaagccggg ctggacatgg ctattttgaa cgcggctgac
     4861 ctcgtgccat acaccgaaat ctccgcggac gaaaaagaag ccactgaacg tctgatcttt
     4921 cacaattccg ctcaggcact ggctgatttc atcgcaatat tcaccgaagg gaagagaaac
     4981 cacgcagagg tggaaagccc ccctatctca acgcgaatgc agcccggaga gaaggtatat
     5041 cggcgcatca caaaccgcac aagagacggg ttggaagagg atatcgtgcg atacatccag
     5101 gagaagtcac ggcagcaatc tccccagcat gccgcgcttt ccctgctcaa cgaacatttg
     5161 ctgcccgcca tgaaagaagt gggcgagcaa ttcagcaacg gtgagattat cttgccattc
     5221 gtactacaat cttctgagat catgcgggca gcgaccacaa aactggaaga gtacatccag
     5281 agacaagagc aagaagaaat gggtcgtgtt ttgttggcga cagtttatgg ggatgttcac
     5341 gatatcggca agaacctggt aagaaccatt ctggaaaaca acgggtatag cgtaatcgac
     5401 ctggggaagc aagttccggc agagcagatc gtggagaccg ctctggacaa aaacgtgact
     5461 gccatcgggc tgagcgcact gttggtaaac accagccagc agatgccatt ggtagtcgaa
     5521 aaactgcgcg ctcagcgcag caacattccg ctgctgatcg ggggtgcagc ggtaaaccag
     5581 accttcgcga aaagaatcga aaaggacgag cagggagctg catatgccgg aggggttttc
     5641 tattgtaagg acgcttttga tgccctcgcc atattgggtg ggctgcaaca aagccggaaa
     5701 gggatgcaat gaacagcgct gatccaacgc ccgttctcgg cgcccacatc attccccaca
     5761 tcgcggtcga agaactgctc ccttttctta atcgcaaagc cctctatcgt atttcctggg
     5821 gagcacaaaa agcacaaggc gctcaatggg aaaaaatcca gaaagaatat gaagggaaac
     5881 tggagagcat gctatccgaa ttgcgcacga acgcgtggat aaccccggca gcaggatacg
     5941 gacattggat ggcaaattct gaaggcgata caattctgct atatgatcca cagaacagcg
     6001 aaaagatcat cgcccgcttt ccgttacccc gccagcaatc cggcgaacga ctgtgcctgg
     6061 cagattatct tcctgcctgc tccagcggga tctccaccat cctatccctg caagtggtca
     6121 gcatggggca caaagccagc gaaacagcca atcgtttttt cagcaatgga aactacgccg
     6181 aggggtattt cagacacgga ctggctgttc aattcgccga agccgcggct gaatatgtgc
     6241 acgccctgat ccgaaaggaa ttgagcctgg aaaagaggca aggacggcga tactcgtggg
     6301 ggtacgagcc tattcccgat ctgagccaac accgcattct ttttgagttg attccggcac
     6361 aagcagagct ggatatggat ttgacctccg cctgccagtt gatccccgaa caatccacag
     6421 cggctatggt cattcaccat aaagccgccc gctattttcg aatattgtaa acaaggactt
     6481 attgtatgaa tagagatgct tttctgaaac gattgcgcga aaagaaacca ctggtagccg
     6541 atggggcaat gggaacattg ctgcacgatc gcggtgtggg ttttgaaagc tgtttcgatg
     6601 aattgaacct gaccaacccc gccctggtcg gatccatcca ccaggaatac atcaaagcgg
     6661 gcgctgaggt cattttgacc aacacgttcg gcgcaaaccg cttcaaactg gaacggtgcg
     6721 gtctggaa
//
LOCUS       DAOSDS010000106         6662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4178_length_6662_cov_4.910852, whole genome shotgun sequence.
ACCESSION   DAOSDS010000106 DAOSDS010000000
VERSION     DAOSDS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4178_length_6662_cov_4.910852"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PLL88_09910"
     CDS             complement(<1..814)
                     /locus_tag="PLL88_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ01921.1"
                     /translation="MANRFEWIQKELKNLKSAGLYNNIRTLGSAQGAWLTVDGKQVLN
                     FCSNNYLGLANHPRLVERAKKVMDEYGVGPGAVRTIAGTMTIHRELESRMATFKHSEA
                     AISFQSGFVANLATITALVGKEDAIFSDELNHASIIDGCRLSGARIVRYDHCSALSLE
                     QVIKENEGNYRRALIVTDGVFSMDGDIAPLDRLYEIANAHDILLMVDDAHGEGVLGKG
                     GRGIVDHFDLHGKVDIEVGTFSKAFGVVGGVVSGNADVVEWLTQRGRPFLSPV"
     gene            complement(862..2490)
                     /locus_tag="PLL88_09915"
     CDS             complement(862..2490)
                     /locus_tag="PLL88_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01922.1"
                     /translation="MPTIKNLLYNHDPDVQRAILTRWGFELPTEISIQDLKSLSSAAI
                     QDGTILEIIQTLPDPAKICLTDLMKNDGKIPWSQFSRKYGEIRVMGAGRRERVHPERE
                     PANLSEVLWYNGLMGKAFFNTKPEPVEYAYIPEEIRELLHQAARKNRPQTGKEFVLPK
                     GILIQRAHDYILDDLCTVLAGLRMGMKIQEIQPMRSPFSSAVVQQLALSMGFYHPRNG
                     LNLDAVQEHLTKPRVESYYDVLQNWQHSHIFPDVLLLSDLDFGKNPHINFLNVRQRMI
                     DLLDLIPSGTWWELDATIADIKTNQPEFLRVAGDMDAWFIQDRQTGELLQGFEYWDEV
                     EGKFIRFFIATILYGLGLCDLAIDRKSGVVLGFRWSERKKALHEIREAPDEEKGAFYA
                     LAQGNLVISPTCSRSLRYQIARFCEWKGMRRGEYQYQITPASLWRARQQDLQVNQLIG
                     LLKKHFTKETVSGLFSALQHFEEEEKFATIQPAILLHIDDEKVLLKIKQSELKKYIIE
                     EINSSTLSIDRKGIEQFQAKLTELGYLCHIAGEV"
     gene            2590..3195
                     /locus_tag="PLL88_09920"
     CDS             2590..3195
                     /locus_tag="PLL88_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01923.1"
                     /translation="MQPALQQGFFQGYLEVLGAELLREAVGEESFSLILEKKEGSFDE
                     PFDAQFFVFFDEVLQKRYGIVASRGIARLAGRLAFKVYKDGIPIFVERGTIENRLLPF
                     VEKITAVLQDLIRLLKEDALADLVTRHITQDDAWAVEGTIWLPNGRFLQVGDQQFFMG
                     MLESMLEWLDSRHVFQVSRKTDSAEFQSDEINLLVSGKRFD"
     regulatory      3255..3362
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3387..4031
                     /locus_tag="PLL88_09925"
     CDS             3387..4031
                     /locus_tag="PLL88_09925"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOJ01924.1"
                     /translation="MNRAVIFANGVLRNYALILPCLQSRDFIIAADGGLKHVHHLKLK
                     PALIIGDLDSISASDLDIAEFQHTEIIRFEKDKDETDLELAIKAAMERGYREILIVAG
                     LGGRLDQTMANLFLMLHPDFKKCNITFNDGVEEVCLVKGTCVIKGNRGDIVSLFPLQG
                     DCEGVKTENLKYPLQNETLTQYKTRGISNVMTAPKATISISKGIALCVHTYKMM"
     gene            4048..5115
                     /locus_tag="PLL88_09930"
     CDS             4048..5115
                     /locus_tag="PLL88_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ01925.1"
                     /translation="MRKKIVISIIVAALSSMFVLSACKGASATDMEQRTLTVLTHDSF
                     AISETVIAQFEQDNNVKVNFVLSGDTGSALNKIILTKDAPLGDVFYGVDNTFLSRALE
                     EEIFESYNSPLLSEVPEQFRLDDQNRALPVDYGDICINYDKSYFANHNLTIPQSLDDL
                     LKPEYKGLLVVENPAVSSPGLAFMLATIAHFGEDGYLDYWRTLKENGVVVVNDWSTAY
                     YTNFSASSGKGPQPMAVSYATSPAAEVIYAAEALEEAPTASIIGEDTCFRQIEFVGIL
                     QNTPNRDLAEKFVDFMLDVFFQEDIPLNMFMLPVNRNAQLPEEFVNYIQIPDAPAALP
                     YEQISASRESWIEDWQEVVLK"
     gene            5122..>6662
                     /locus_tag="PLL88_09935"
     CDS             5122..>6662
                     /locus_tag="PLL88_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOJ01926.1"
                     /translation="MLHFTRRRIFFLLGLITTLFLAAFYVLPLGVILQKAFEGFRAFL
                     LVDGLLKKIGSPLVFTVYQAGLSVLLTVVVGLPTAYAFSHFDFKGKNFFRTAVTLPFI
                     LPTVVVAAAFNSLLGSNGWLNLLLMRVFHVETSPLQIMNTLTVILLAHVFYNTSVFVR
                     VVGAAWAQLETEQEQAASVLGANAWQRFYWITLPLLKKSIVSALLMVFLFDFTSFGVI
                     LLLGGPAYNTLEVEIYLQTTQLLNLPLAGVLSVVQLLVTFLISWSLLRDTHTGMVTFL
                     PDVKNERIKKPATGQQKILLALLLIFLFLFFVLPEISLFFRSVVMVDTAGTAKFTADF
                     YYELFQNTRASLLYISPFRALKNSLSYALVTMVLAGILGVMAAYGLLNSKRVKLLRAL
                     LILPLGTSSVTLGLGFLLTFTGVSLFQRTPQILIPIAHTLVALPVVINTLYPAFSAIP
                     ENLRDAAGNLGAAPLQILSKIDLPLVRRSILVSALYAFILSLGEFGATSFLTRPEYPT
                     MPVAI"
BASE COUNT         1586 a         1571 c         1619 g         1886 t
ORIGIN      
        1 gcactggaga aaggaaaggt ctgccgcgtt gggtcagcca ttccaccacg tcggcattcc
       61 ccgagaccac gccccctacc acgccaaaag cttttgaaaa cgttcccact tcgatatcca
      121 ctttcccgtg caaatcgaag tggtcaacaa ttccacgccc gcccttgcca agcacccctt
      181 caccgtgcgc atcatctacc atgagcaaga tatcatgcgc attggcaatc tcatacaacc
      241 ggtcgagggg ggcgatatcc ccatccatgc tgaatacgcc gtctgttaca atcaaagcgc
      301 gccggtagtt gccttcattt tctttgatga cctgttcaag gctcagggca ctgcaatgat
      361 cgtagcggac gatgcgcgct cctgagagcc ggcagccatc gatgatgctg gcgtggttca
      421 gttcatccga aaagatcgca tcctcttttc caaccagggc ggtaatagtg gcgaggttgg
      481 caacgaaacc ggactggaag gagatggctg cctcggaatg tttgaacgtt gccatgcgac
      541 tttccaattc gcggtggatg gtcatggtgc cggcgatggt gcgcacggct ccaggtccga
      601 cgccgtattc atccatgacc ttttttgcgc gctcgaccag gcgtgggtgg tttgccaaac
      661 ccagatagtt gttcgagcaa aagttcaaga cctgcttgcc atccacggtc aaccaggcac
      721 cttgcgcgga acccagcgtg cgaatgttgt tatagagacc agcgcttttt agatttttca
      781 gttctttttg aatccactca aacctgttcg ccatgttcac ctcgattttc ataatggatt
      841 tgaaaccaga tttcagggag attatacttc cccagctata tggcacaagt aacctaattc
      901 agtaagtttt gcctgaaatt gttctattcc ttttcgatca atactaaggg tggaggaatt
      961 tatctcctca atgatatatt ttttcaattc ggattgcttg attttgagaa gcaccttctc
     1021 atcatcgatg tgcagcaata tggcaggctg gatggtggcg aacttttcct cttcttcgaa
     1081 atgctgtaaa gcactgaata accctgatac ggtctctttg gtgaaatgct ttttcagcaa
     1141 tccgatcaac tggttgacct gcaggtcttg ctgcctggct cgccacaaag aagcaggggt
     1201 tatctgatat tgatattcgc cccggcgcat ccctttccat tcacaaaagc gcgcgatctg
     1261 atatcgcaaa gagcgagaac atgtcgggct gattaccaga tttccctgtg ccagcgcgta
     1321 aaaagcgcct ttctcctcat caggagcctc ccgtatctcg tgcagggctt tcttccgctc
     1381 agaccatcgg aaacccaaaa caactccgct tttccggtcg attgccaggt cgcataatcc
     1441 gagaccatat aatatggtgg caatgaaaaa gcgaatgaac ttcccttcta cttcatccca
     1501 gtattcgaaa ccttgtaaaa gctcgcctgt ttggcgatct tgtataaacc atgcatccat
     1561 gtcccctgcc actcgcaaga attccggttg attggttttg atatctgcaa tggtcgcatc
     1621 cagttcccac caggttcctg atggaatgag atccagcagg tcaatcatgc gttggcggac
     1681 gttcagaaaa ttgatatgcg gattctttcc gaagtccaaa tcagacagca gcaacacatc
     1741 gggaaatatg tgactgtgtt gccaattttg cagcacgtcg taataacttt ccacgcgcgg
     1801 ttttgttaaa tgctcttgaa cggcatccag attgagtccg tttctgggat ggtaaaaacc
     1861 catggagagc gctaattgtt gtacgactgc agaagagaaa gggctccgca tgggttggat
     1921 ctcttgaatt ttcattccca tacgcaagcc tgccaacact gtgcaaagat catccagaat
     1981 gtagtcatgt gcacgctgga tgaggatacc ctttggcagg acaaactctt tccctgtttg
     2041 gggtcggttc tttcttgctg cctgatgaag aagctcgcgg atttcttccg gaatataggc
     2101 atattcgacc ggttccggct tggtgttgaa gaaggctttt cccattaacc cgttatacca
     2161 aagcacttcg ctcaaattgg ctggttcccg ctcaggatgg acacgttctc ttctgcctgc
     2221 acccatcacg cggatctccc catatttgcg actgaattgt gaccagggta tttttccgtc
     2281 gtttttcatg aggtccgtga ggcatatttt tgccgggtca ggcagggtct ggatgatctc
     2341 gaggattgtt ccatcctgga tcgctgcgga tgagagtgat ttcagatctt gaatggaaat
     2401 ctccgttggc aactcaaacc cccagcgcgt aagaattgca cgctgaacat caggatcgtg
     2461 gttatagagt aggttcttta tggtgggcat atttcgtcat cccactctga ttttaaccgc
     2521 aaaaattaga agaagattgt tgttataatt ttgaagagaa ttcaacaatc aaaaaggatc
     2581 ttagaattta tgcaacctgc tctacaacag ggcttttttc agggatatct ggaagtattg
     2641 ggggctgaac tactgcgcga agcagtaggt gaggaatcct tttctttgat ccttgaaaag
     2701 aaagagggtt catttgatga accatttgat gcgcaatttt ttgttttttt cgatgaagtg
     2761 ctccaaaaga gatatgggat tgttgcatcg cgagggatag cgcgtttggc gggtcgcctg
     2821 gcttttaaag tttacaaaga tgggatccca atctttgttg agcggggcac tatagaaaat
     2881 cgactgctgc catttgtcga gaagatcacc gcagttttac aggacttgat caggctgttg
     2941 aaagaggacg ctctggctga tctcgtaaca cggcatatca cccaagatga tgcctgggct
     3001 gtggaaggga ctatctggct tccaaatggc aggttcctgc aggtgggcga tcagcaattc
     3061 tttatgggaa tgctggaaag tatgctggaa tggctggaca gccggcatgt gttccaggtt
     3121 agccgtaaaa ctgattcggc tgaatttcaa tccgatgaaa tcaatctttt ggtctctgga
     3181 aaacgatttg attaaacgca gatttgacag atcatcggtt attgcctata cttaacccgt
     3241 aaacatcaag aaaaggaaca gatccgggga gccatgaggt ggctgagagg aagagcaaaa
     3301 tcttcgaccc gtataacctg atccggttaa taccggcgga gggagcataa agaaaacgaa
     3361 ctccctcgcc aacgagggat ttttttatga acagagcagt gatctttgcc aatggggtct
     3421 tgaggaacta tgcactcatt ctcccttgtt tgcaatcgcg tgattttatc attgctgcgg
     3481 atgggggatt gaagcatgtt catcatttga aattgaaacc agcattgatc atcggcgatc
     3541 tggattccat ctccgcttct gatctggata ttgctgaatt tcaacacacc gagatcattc
     3601 gctttgagaa agacaaagat gaaacggatc tggaactggc tatcaaggct gccatggaac
     3661 gcggctatcg agagatcttg attgttgcgg gattgggagg gcgattggac caaaccatgg
     3721 caaatctttt cctcatgttg caccctgatt tcaaaaaatg caacatcact ttcaatgatg
     3781 gggtcgaaga agtatgtctt gtaaagggaa cgtgcgtgat caagggaaat cggggcgata
     3841 ttgtttccct cttccctctg cagggtgatt gcgaaggggt gaaaaccgaa aatttgaaat
     3901 atcctctgca aaatgaaacc ctcacccaat acaagacgcg cggtatcagc aatgtgatga
     3961 ccgctcccaa agcgactatc tcgatttcca aagggatcgc tttatgtgtt catacatata
     4021 agatgatgta gatgtaaagg agagacgatg agaaagaaaa ttgtcatttc aataattgtt
     4081 gctgcgctca gcagcatgtt cgttctcagt gcatgcaagg gtgcgtcggc aaccgatatg
     4141 gagcagcgta ctctgaccgt attaacccac gattcttttg ccatcagcga gacggtgatc
     4201 gctcaatttg agcaggacaa taacgtgaag gtcaattttg ttctcagcgg agatacgggc
     4261 agcgctttga acaagatcat cctgaccaag gatgctccat tgggtgatgt tttttatggg
     4321 gtagataaca ccttcctttc ccgcgctttg gaagaagaaa tctttgaatc ctacaattca
     4381 ccattgctgt cagaggtccc tgaacagttt cgattggacg accagaaccg tgccctgcca
     4441 gtggattatg gagatatttg cattaattat gacaaatcat attttgcaaa ccacaatctc
     4501 accattccgc agagcctgga tgatctcttg aagccagagt acaaagggtt attggtggtg
     4561 gagaatccgg cagtatcttc tcccggattg gctttcatgc ttgctaccat tgcacacttt
     4621 ggagaggatg gctatctcga ctactggcgg acattgaagg agaatggtgt ggtggttgtt
     4681 aacgattgga gcacagctta ctatactaat ttttccgctt catctggtaa aggtcctcaa
     4741 cccatggcgg tttcttatgc taccagcccg gctgctgaag tgatctatgc tgccgaagcg
     4801 ttggaagaag cgcccaccgc atccattatt ggcgaggaca cttgcttccg ccagatcgag
     4861 tttgtgggta tcctgcaaaa cactcccaat cgcgacctgg ctgaaaaatt cgtggatttt
     4921 atgctggatg tgttttttca ggaagatatc cctctgaaca tgttcatgct gccggttaac
     4981 aggaatgctc agttaccgga ggaatttgtt aactacatcc agatccccga cgctccagcc
     5041 gcattacctt acgaacagat ctctgcatcg cgcgaaagct ggatcgagga ttggcaagaa
     5101 gtggtcttaa agtaaggcaa tatgctccat ttcaccaggc ggcggatctt ttttcttctc
     5161 gggttgatta ccaccctgtt tctagcagca ttctatgtgc tgccgcttgg tgtcatcctt
     5221 caaaaagcgt ttgagggatt ccgcgcattc ctccttgttg atgggttgct caaaaagatc
     5281 ggcagcccat tggtctttac tgtttatcag gcggggttat ccgtcctttt gacagtcgtg
     5341 gtgggtctgc cgaccgccta tgcgttttcc cattttgatt tcaaggggaa aaattttttt
     5401 cgcacagcag tcacattacc ttttattctg cccacggtgg tggtagcggc tgcttttaac
     5461 agtctgctgg gatcgaatgg atggttgaac ttgttgttga tgcgtgtttt ccatgtggaa
     5521 acatcgcccc ttcagatcat gaacactttg accgttatcc tgcttgctca cgttttttac
     5581 aatacctcgg tgttcgtaag ggtggtggga gctgcctggg cgcaattgga aaccgagcag
     5641 gaacaggcag ccagcgttct tggcgcaaat gcctggcagc gtttttattg gatcacgctc
     5701 ccgctcttga aaaaatccat cgtttccgcc ttactgatgg tgttcctgtt tgatttcacc
     5761 agttttgggg tcattttgct gctgggcggt cctgcctata atacgctcga agtggagatc
     5821 tatttgcaaa ccacgcagtt gctcaatctg cctttggcgg gagttttaag tgttgtgcaa
     5881 ttgcttgtga cctttttgat ctcctggtct ttgctgcgcg acacgcacac cggcatggtg
     5941 acttttttac cggacgtcaa aaatgaacgc atcaagaaac cagccactgg acaacaaaaa
     6001 atcctccttg cgttgctgct gatattcctg ttcctttttt ttgtcttgcc tgagatcagt
     6061 ttgttttttc gttctgtggt gatggtggat acagccggaa cagcaaaatt cactgctgat
     6121 ttttattatg agctctttca aaatacccgt gcttcgcttt tgtacatttc ccctttcaga
     6181 gcattgaaaa actctctcag ttatgctctg gtaaccatgg tgttggcggg tatattgggc
     6241 gtcatggctg cttatggatt gttgaattcc aaacgggtca aactgctgcg cgcattgctg
     6301 atcctgccat tgggaacttc ttcggttacg ctgggattgg gttttctgct gactttcacc
     6361 ggcgtaagct tgtttcagcg gactccccaa attttgatcc ccattgccca taccctggta
     6421 gcgctgccgg ttgtcatcaa cacgctctac ccggcttttt ctgccattcc ggagaatttg
     6481 cgcgatgccg ctggaaattt gggagcagct ccactgcaaa tactgagcaa aatcgacctg
     6541 cctctcgttc gtcgttcgat actggttagc gcactgtatg cgttcatcct ttcgctggga
     6601 gaattcggtg ctacttcctt cttgacccgt cccgaatacc caaccatgcc ggtggctatc
     6661 ta
//
LOCUS       DAOSDS010000107         6609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4206_length_6609_cov_4.046842, whole genome shotgun sequence.
ACCESSION   DAOSDS010000107 DAOSDS010000000
VERSION     DAOSDS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4206_length_6609_cov_4.046842"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PLL88_09940"
     CDS             <1..242
                     /locus_tag="PLL88_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01927.1"
                     /translation="GIGCTSVSDPDLVYLEHFGQPQLPLARQKMMAATAPAPTPPAED
                     VLAVPSYLRNRQIPLELIEEEYAKKHLRSDLQPDL"
     gene            199..417
                     /locus_tag="PLL88_09945"
     CDS             199..417
                     /locus_tag="PLL88_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01928.1"
                     /translation="MQKNIYDLICSQIYKKYPAVKHSKPEVKTQPNGDYLLIFGASQK
                     TANGHSLPTTLRVTADDKGHIKKVSSSK"
     gene            complement(547..1734)
                     /locus_tag="PLL88_09950"
     CDS             complement(547..1734)
                     /locus_tag="PLL88_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOJ01929.1"
                     /translation="MDFNFSTATRIVFGRAKVREVAAAVPGMGERAMLVTNRIKEADY
                     TNLDAIRERGFDIEKELNSKGVETFRYLVSGEPSIEMVEEGILFAKEKGMNVFVGIGG
                     GSAIDTAKAISAVMANGGECLDYLEVIGKGKKITKKSLPFVAVPTTAGTGAEVSANAV
                     LASMEHNVKVSLRSPLMIADLAIVDPELTISVPPAVTASTGLDAFTQVIEPFVSRMAN
                     PITDALCREGMKRASRSLYKAYLDGEDIEAREDLCIVSLFGGICLANAKLGAVHGFAG
                     PLGGLYHGAHGAICAALLPHVVEVNVAALRERDAESPILERYREVARIVTTDQNASVE
                     DGVRWIAELVKSMQIKGLAEYGMKEEDFPRIITMSSASSSMKGNCIELNEKEMREILS
                     RAL"
     gene            complement(2011..2436)
                     /locus_tag="PLL88_09955"
     CDS             complement(2011..2436)
                     /locus_tag="PLL88_09955"
                     /inference="COORDINATES: protein motif:HMM:NF014762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HOJ01930.1"
                     /translation="MENLSASMQHYVKAIYELSSGCGGVRISDIATKLEVTKASSSVA
                     IKALQEKKMVYRDAHRLVFLTEDGEYQAILALDKFAIIRGFLTEVLDVEPAIADSDAC
                     AMEHVISTETLCTFCRRTNRKCTTGCHAKADAAPEKAGN"
     gene            complement(2506..3192)
                     /locus_tag="PLL88_09960"
     CDS             complement(2506..3192)
                     /locus_tag="PLL88_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ01931.1"
                     /translation="MMFLEIQGLSKQYKRGEKAFLAVGSVDLSIDQGDFVSIIGRSGS
                     GKSTLLNMIAGLLTPTAGNIKLEGTDIHALNDKQISELRNTRMGYIPQGSGVLSNLTI
                     FDNVRLPYYLSKRQGDAAGRASFILDEIGMSSLADMLPAQLSGGEIRRVLIARALMNE
                     PDILIADEPTSDLDIETAKEIMKLFARINKSGTTILIVTHELDTLEFGNRVLSMASGR
                     LTEGIQLTYN"
     gene            complement(3189..4400)
                     /locus_tag="PLL88_09965"
     CDS             complement(3189..4400)
                     /locus_tag="PLL88_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOJ01932.1"
                     /translation="MDRKPLSTVNIALINLKRKPLRTGGLILLVSLFAFTLFGGTVLS
                     KSLENGLSNLSKRMGADILAVPYGYEADVQSALLRGEPSTFYFEAAITEKIAGVEGVQ
                     NVSPQLYIATLNAGCCSYPIQLIGFEPETDFIIQPWMSNFLDHPLADGEIVIGNSLNA
                     QVGQKLIFFNQTFLIVARLDKTGMGFDTSVFMNLSTAKQMARESERMKAHPVAENADL
                     ISSVMVEIKDGYDVKEVANAILQAYAGEGVHVVVAKNMISDISGSLRGLTTYIFTLAG
                     VLWVLAVGVLIIVFAITLNGRKREFSIYRVLGAPRSKLAKLILCESSLISLFGTVGGI
                     IAATLVVFPFSTYISSLLEYPYLQPSYGTLLAVAVVSSLVSFAVGPLASAYSAIKIGR
                     CEIYTAMRENE"
     gene            complement(4411..4830)
                     /locus_tag="PLL88_09970"
     CDS             complement(4411..4830)
                     /locus_tag="PLL88_09970"
                     /inference="COORDINATES: protein motif:HMM:NF025745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4418 family protein"
                     /protein_id="HOJ01933.1"
                     /translation="MKNRFFSGMVVAILGLLIILVPVWIFPPCSEVIETAAGNMRMKC
                     FWSGQAEIGIGFLILCGGALLIVFKSPLIRLGLSVMLSLIGVLGILIPTLLIGGCEMA
                     TMPCRMTTFPALIILNLLMGGFSAANALHLWRQNKKP"
     gene            complement(4836..5864)
                     /locus_tag="PLL88_09975"
     CDS             complement(4836..5864)
                     /locus_tag="PLL88_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOJ01934.1"
                     /translation="MHFFDEGKESTMTVTIAEEKAEKIFCAMGTLMSFTVYGAYAQEA
                     LKAAEAITVKLDNLLARTKPDGEITRLNHSGGKDWTKVQEDTDSVLREALRYAEKTQG
                     VYDPSIGALVDLWNVKARREKNLPDLKEIRCALASCDYRNIETNHAGGYRIQHGASID
                     LGGIGKGYAADRICALCRERDALSALFSLGTSSIAALGSKPDGSAWKIGLRAVGTDNI
                     ECFGVVHLKDQFLSTSGDYEQGFVKNGHRYHHILDKRSGYPAESGLRSVTVIANSGAM
                     SEAYSTALFVMGLDKALDFHQREGEFEMIVVTSDSHVICTPKLQEMFEFTGETLGYEY
                     PQYELFRR"
     gene            complement(5907..6338)
                     /locus_tag="PLL88_09980"
     CDS             complement(5907..6338)
                     /locus_tag="PLL88_09980"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOJ01935.1"
                     /translation="MKRNLISMIFTLAFTLQLMGCNNSYRDVTYKDGVYIGKSSEDDL
                     GAYGEVRITIEEGSIAECEYVTWQKDGSIKDENYGKVNGEISNQDYYDKAQLAVKAMT
                     QYAEKLVEAQSLKGVDAISGATISYSQFVEAVNNALDQVQE"
     gene            complement(6335..>6609)
                     /locus_tag="PLL88_09985"
     CDS             complement(6335..>6609)
                     /locus_tag="PLL88_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ01936.1"
                     /translation="LSGGEQQRVCIARALINYPMLILADEPTGNLDETNESIVLDIFK
                     QLHKEGSTIMIVTHDPEVAQRAQRTVVLEHGRVARIVQNKNERYTR"
BASE COUNT         1540 a         1872 c         1496 g         1701 t
ORIGIN      
        1 ccggcatcgg ctgcacctct gtcagcgacc ccgacctggt ctatctcgag catttcgggc
       61 aaccgcagtt gccgctcgcc cggcagaaaa tgatggctgc caccgctccc gctcctacgc
      121 caccggctga ggacgtcctg gcagtgccct cgtatttgcg caatcgtcag attccgctag
      181 aattgattga ggaggaatat gcaaaaaaac atttacgatc tgatttgcag ccagatctat
      241 aaaaagtacc ccgccgtcaa gcacagcaag cctgaggtca aaacccagcc caatggcgat
      301 tatctgctca tctttggcgc cagccagaag accgctaatg ggcattccct gcccaccacc
      361 ctgcgcgtca ctgctgatga taaggggcac atcaaaaagg tcagcagctc gaaatagcgc
      421 tcttttcggc agtcatgaca tgccagagcc tggtgagcaa tcggagcctt taaacttccc
      481 aataaaaaac ggaagctgtg cttcttcaac gctcccgttt tctttcaatt tgtggggaca
      541 cctgttttat aaagctcgtg atagaatctc gcgcatttct ttttcgttca gttcaatgca
      601 gttgcctttc atgctgctgg aagccgaaga catggtgatg atgcgcggaa aatcctcttc
      661 tttcatccca tattccgcca accctttgat ttgcatggac ttgaccagtt ctgcgatcca
      721 gcgcaccccg tcctccacac ttgcgttttg atcggttgtg acgatgcgcg ccacctccct
      781 gtatcgctcc aggatcgggc tttccgcatc gcgttccctc agcgccgcca cattgacctc
      841 caccacatgc ggcagcagtg cagcgcaaat ggctccgtgc gctccatggt acaggccgcc
      901 cagcggaccg gcgaaaccgt gcaccgctcc tagtttggca tttgccaggc aaatgccccc
      961 gaacaaactg acgatgcaca gatcttcgcg cgcttcgatg tcctccccat cgagatacgc
     1021 tttatacagc gatcggctgg cccgtttcat cccttccctg cacagcgcgt ctgtgatcgg
     1081 attggccatg cgcgagacga atggctcgat cacctgggtg aaagcgtcca gaccggtgct
     1141 ggcggtaacc gctggtggta cgctgatggt caactcaggg tccacgatcg ccagatcggc
     1201 gatcatcaat gggctgcgca ggctcacttt cacgttgtgc tccattgacg ccagcactgc
     1261 gttagccgac acctccgcgc ccgtccctgc cgtggtcggc acagccacga acggcagcga
     1321 ttttttggtg atcttctttc cttttccaat cacttccaga taatccagac attccccccc
     1381 gtttgccatc accgcgctga tcgcctttgc cgtgtcaatg gcgctccctc cgccgatccc
     1441 cacgaacacg ttcattccct tttctttggc aaataagatt ccttcttcca ccatttcaat
     1501 gctgggttct ccgcttacca aatagcggaa ggtctctacc cccttgctgt tcaattcttt
     1561 ttcaatgtca aatccgcgct cccgtatcgc gtcgaggttt gtgtagtccg cttctttgat
     1621 cctgtttgtc accagcattg cgcgctcgcc catgccgggc accgctgccg ccacttccct
     1681 cactttggca cgaccaaaaa cgatgcgcgt cgcggttgaa aagttgaaat ccatcgctgc
     1741 tcctttaaaa taaaaccaaa atcgctctct tgaatgccca tctgtcgaac atattgttat
     1801 taaacaagca ttcggaacac tgtagaaatt ataccggcaa tccccattac cggtcattct
     1861 ctccccgcat agtgctcttc cttcagggtc aaatttcctg gttttatgaa gaatctcaac
     1921 gtctatttcc gaagggagaa aaacaaatgc cctttcacca acaagggttg agaaagggca
     1981 tttgcttatc gcacgtcatg gaaattcaca ttagtttccg gccttctcag gggcggcatc
     2041 tgccttcgca tggcatcctg tggtacattt tcgatttgtg cgacggcaga aagtgcacag
     2101 cgtttccgta ctgatgacat gttccattgc acaggcgtcg ctatccgcga tcgcaggctc
     2161 tacgtcaagc acctcggtca aaaacccgcg tatgattgca aatttgtcta aagctaaaat
     2221 cgcctgatat tccccatctt cagtgagaaa gaccagtctg tgagcatccc gatataccat
     2281 ctttttctct tgcagggctt ttatcgccac gctcgaactt gcttttgtga cctctagctt
     2341 agtcgcaata tctgagatgc gcacacctcc acatccagag gagagctcgt aaattgcttt
     2401 tacataatgt tgcatagaag cagaaagatt ttccatattg ctccattata aatcacaacg
     2461 ctgcccgtcc aaacgctgag ctttcaaccg caaatgagag gttttttagt tataggttaa
     2521 ttgtatcccc tccgttaacc gtcccgacgc catgctcagc accctgttcc caaattccaa
     2581 tgtgtccaat tcgtgggtaa cgatcaaaat cgtcgtgccg cttttgttga tccgggcaaa
     2641 aagcttcatg atttctttgg ctgtctcgat atccaaatct gatgtaggct catccgcaat
     2701 caaaatatcc ggctcgttca tcagcgccct tgcaatcaga acacgccgta tttctccgcc
     2761 cgagagttgg gcgggcagca tatcagcgag ggatgacatg ccgatctcat caaggataaa
     2821 cgacgccctc cccgcggcgt ccccctgcct tttggaaaga taataaggta atctgacgtt
     2881 atcaaaaatg gtcaggttgg aaagcacgcc ggacccttgc gggatgtatc ccatccgtgt
     2941 gtttcgcagc tccgagattt gtttgtcatt taaggcatgg atgtctgtgc cctccagctt
     3001 gatattgcct gccgtgggtg ttaaaagccc ggcgatcata ttgagcaggg tgctcttgcc
     3061 gcttcctgaa cgcccgatga tgctgacaaa gtcgccctga tctatagata gatccacgct
     3121 acccaccgcc aggaatgctt tttccccgcg tttgtattgt ttgcttagcc cttgaatttc
     3181 caaaaacatc attcattctc cctcatggca gtgtagattt cgcagcgtcc aattttgatt
     3241 gcggaataag ccgaggcaag cgggccaacg gcaaaggaca ccagggaact cacaaccgcc
     3301 accgccaaca aggttccgta ggaaggctgc aaataggggt attccaacag cgagctgata
     3361 tacgtgctga acgggaaaac aacgagggtc gcagctatga ttccgcccac cgttccgaaa
     3421 agactgatca aagaggactc acaaagaatt aattttgcca gtttgctcct tggcgcgccc
     3481 aagaccctgt atatgctgaa ctcccttttc cgtccgttta aggtgatggc gaagacgatg
     3541 atgagcacgc cgaccgccag cacccacaga acgcccgcca gcgtaaaaat ataggtggta
     3601 agcccgcgca agctgccgga gatatcgctg atcatgtttt tggcgactac cacatgcacg
     3661 ccttctccgg cgtaagcctg gaggatggca ttggcaacct ctttgacgtc ataaccatct
     3721 ttaatttcaa ccatgacgga tgaaatcagg tcggcgttct ccgctaccgg atgcgctttc
     3781 attctctcag attcccgtgc catctgtttc gccgtagaaa ggttcatgaa caccgaggta
     3841 tcaaagccca tgcctgtttt gtccagacgg gcgacgatta aaaaagtctg attgaagaat
     3901 ataagttttt gcccaacctg agcgttgagg ctgttgccga tcacaatctc gccatccgca
     3961 agaggatgat ccaagaaatt tgacatccac ggctggatga taaaatcggt ttccggttca
     4021 aagcctatga gttggattgg gtaagagcag cacccagcgt tcaacgttgc aatataaagc
     4081 tggggcgata cattttgtac gccttcaacc cccgcgattt tttccgttat tgcagcctca
     4141 aagtagaaag tgctgggttc tccccgcagc aacgcgctct gcacatccgc ttcatatcca
     4201 tatggcacag ccaggatatc cgcacccatt cttttcgaca ggttgctcaa gccattttcc
     4261 aggcttttcg acaacacggt tcctccaaaa agtgtaaagg caaatagtga cacaagaagg
     4321 atcagcccgc ctgttcgaag cggcttacgt tttaggttaa tcagcgcgat gttaacggtg
     4381 gataacggtt tcctatccat gcatgctttc ctatggtttt ttattctgcc gccagagatg
     4441 cagagcattt gcagcagaga accctcccat taaaagattt aaaatgatca acgcggggaa
     4501 ggtcgtcatt ctgcagggca tagtagccat ctcgcatccg ccgattaaca gggtggggat
     4561 cagaattccc aagacaccga tcaaagagag catcacgctg agtccaagcc ttatcagcgg
     4621 cgacttgaaa acaatcaata gcgctccgcc gcacaggatt aaaaatccaa ttccaatttc
     4681 agcttgcccg ctccaaaagc acttcattcg catgttgcca gccgcagttt caatcacctc
     4741 agaacaaggc ggaaaaatcc acaccggcac cagaatgatc aacaacccta aaatggcaac
     4801 gaccatacca gagaaaaatc tgttcttcat aacaattacc tcctgaataa ttcgtattgg
     4861 gggtattcat accccaatgt ttcgcctgtg aattcaaaca tttcttgcaa cttgggcgta
     4921 cagataacat ggctatccga agtgaccacg atcatttcaa attccccttc gcgctgatga
     4981 aaatcaagcg ctttgtccag tcccattaca aacagcgcag tggaataggc ttcgctcatg
     5041 gcgccgctgt ttgcaatgac cgttacggag cgcaacccac tttctgccgg atagccgcta
     5101 cgcttatcga gaatgtgatg atagcgatgc ccgtttttca caaaaccctg ctcataatcg
     5161 ccggaagtcg agagaaattg atcttttaag tgaacaactc cgaagcattc gatattgtct
     5221 gtaccaactg ccctcagtcc gattttccat gcagagccgt ccggctttga gcccaatgcc
     5281 gcgatagatg aggtgccgag agagaagagc gcggaaagag catctcgctc tctgcacaaa
     5341 gcgcagattc tatcggcagc gtagcctttt ccaataccgc ccaggtcaat gctcgcgccg
     5401 tgctgtatcc ggtacccgcc tgcatggttt gtctcaatat tgcgataatc gcaggaggca
     5461 agggcgcacc tgatttcctt caggtcgggg agatttttct cccgcctggc tttcacgttc
     5521 cataaatcca ccagtgcgcc gattgaaggg tcataaacgc cctgtgtctt ttcagcatag
     5581 cgcaaagcct cgcgcagaac gctgtctgtg tcctcctgca cttttgtcca gtcctttcca
     5641 ccggagtgat tcaagcgtgt gatctcaccg tctggcttcg tgcgtgcaag caggttgtct
     5701 agcttcacag tgatcgcttc tgccgctttg agtgcctcct gcgcgtacgc cccgtaaacg
     5761 gtaaagctca tcagcgtgcc catcgcacaa aaaatctttt cagctttctc ttccgcgatg
     5821 gtgacggtca tagtcgattc cttcccttcg tcgaaaaagt gcatgcgcag ctccgctagt
     5881 catcagattc tcataccaat tgccgctcat tcttgcacct ggtctaaagc gttgtttacg
     5941 gcttcaacaa actggctgta ggaaatggtg gcgccagata tcgcatctac acctttcaag
     6001 ctttgggctt cgacaagctt ttccgcatat tgtgtcatcg ctttcacggc aagctgcgcc
     6061 ttgtcgtaat agtcttgatt ggagatttca ccgttcacct tgccgtagtt ttcgtctttt
     6121 attgagccat ctttctgcca cgtgacatat tcacattccg cgatgctgcc ctcttcaatc
     6181 gtgatcctta cctcgccgta agcgcccagg tcgtcttcac tgcttttgcc gatgtaaacg
     6241 ccgtctttgt aggtaacatc gcggtaactg ttgttgcacc ccatcaactg taacgtgaac
     6301 gccagggtga aaatcatgga aataaggttt cttttcatcg ggtatacctc tcatttttat
     6361 tttggacaat tcttgccacc cgcccatgct ccaagacgac ggtccgttgg gctctctgag
     6421 cgacctcagg gtcatgggtg acaatcatga tggtgctgcc ttctttgtga agctgtttga
     6481 aaatgtcaag cacgatgctc tcattcgttt catccagatt tcctgtgggc tcatctgcca
     6541 gaatcagcat ggggtagttg atcagagcac gggcaatgca cactctttgc tgttcgccgc
     6601 ccgaaagct
//
LOCUS       DAOSDS010000108         6595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4218_length_6595_cov_3.916055, whole genome shotgun sequence.
ACCESSION   DAOSDS010000108 DAOSDS010000000
VERSION     DAOSDS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4218_length_6595_cov_3.916055"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLL88_09990"
     CDS             <1..251
                     /locus_tag="PLL88_09990"
                     /inference="COORDINATES: protein motif:HMM:NF037014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01937.1"
                     /translation="RTLLVSELSSIEPLHEDILVDGKLVYDFPSLQEIRSIREKDMAD
                     LYPGVKRLVMPHNYHVSLSQKLWDLKHTLVLKATHEDQ"
     gene            275..1174
                     /locus_tag="PLL88_09995"
     CDS             275..1174
                     /locus_tag="PLL88_09995"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HOJ01938.1"
                     /translation="MEVFEIIHPDFKSLVDIKRHPEIIQSEFQFTEGPVWHAQQHCLY
                     FSDIPANTLYCYSEQKGVEIAFQPSGFSNGLTLDPSGAILACEHRTRAITRYAESEKT
                     ILADTYQGKKLNSPNDIVVTKQGLIYFTDPTYGLREGNGGPAEAELPFQGLYCYRTDW
                     AEPKLVCADFERPNGVALSNDQKTLFVIDTVKQHIRSFQIQEDGSLVDGRVILELWGK
                     GPDRPDGLKLDEFDNIFSTGPEGIWVFRSDHALLGKIRFPQKTANLAFGGEDRSSLFI
                     TSSQYLIRLRLKTRGLTPLDLSK"
     gene            complement(1177..2124)
                     /locus_tag="PLL88_10000"
     CDS             complement(1177..2124)
                     /locus_tag="PLL88_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HOJ01939.1"
                     /translation="MIPGIDVSHWQGDIDWAKVKAAGVKFAFIKASEFPDKKTTMFVD
                     NKFEINRKGAEANGIYWGAYHFFRTHIDSIIQAESFCKLVGSVPSLPLVLDLEVAGSK
                     GSKLVRKVQSFVETITSITGRKPIIYTSGGFWRPYMTYEKLTDVDWAVEYPLWMARYT
                     SQWPGTIYPWSAWDFWQYTDKGRVPGIKASVDLNWFVGSLDDLQTRFLPKTSQSKSTE
                     MLDGEGIAVPSQPFMEEKAFQQPSAPAIAQNIPVPQFRAERIGRQEESEDTKVETQNK
                     QSPSFLSFLHDRFNKKEKRNVDPLQSQEWIEDVVYRDRK"
     gene            complement(2128..2745)
                     /locus_tag="PLL88_10005"
     CDS             complement(2128..2745)
                     /locus_tag="PLL88_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HOJ01940.1"
                     /translation="MIIGIDISEKNNVTSWVEVAKNQVRFVYLKCTDGIAGTVATYAG
                     NKKNAKQTGILTGAYHWLNPAQDAVVQAEHFIKNAAIVDDDLPPVVCLELYSTSKTVL
                     EPKLRTFLETAETRIGKRPLVYTSATYWKKYMAGIEWPCEYPLWIDEPGVIWPAQLFP
                     WAGWAIWQFSYQVKIPSVSGTPGLNWFNGSQADLQALANEGLVKR"
     gene            2840..3232
                     /gene="ssrA"
                     /locus_tag="PLL88_10010"
     tmRNA           2840..3232
                     /gene="ssrA"
                     /locus_tag="PLL88_10010"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3256..3930
                     /gene="cas5"
                     /locus_tag="PLL88_10015"
     CDS             3256..3930
                     /gene="cas5"
                     /locus_tag="PLL88_10015"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HOJ01941.1"
                     /translation="MVRLSYPIEMEISGNTAMWTRPDTGDCPVSYPAPTYSAVKAIFE
                     SILWGPAVEIIPIKVEICTPIQYHAYVTNYGGPLRKNIAINKNNNYQLLATVLIDVCY
                     KLYAEVRPNKEKSKLPQRAKQWDNKTTSPGHAYQAMFNRRLKCGQCYSIPALGWREFT
                     PSYFGCLRSDTEVLSELAPVSIPSMLREVFPGGYHSSVSFVYDQDLVIKNGCLTYPVK
                     GGSHAE"
     gene            3920..6082
                     /locus_tag="PLL88_10020"
     CDS             3920..6082
                     /locus_tag="PLL88_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01942.1"
                     /translation="MLNELHSLSDALSGMSIPTKEWHREYKLIPKVTAKAPCIRIWIG
                     EDGSIYGFDNIDAELSMVIRKYGNNQNSFPAFNVAPLYRITDKQIIKELEQTTKDHAL
                     LDFDEIKSWCTEDNWRMSIVQKVNNCISKSALELQEYIRQQAPAQENSITALISSLQV
                     YSDTDDGGKVFREKLEACIFSKLRVGESIEAALAILLHKGNSEAKDPEKDSGSLSVIL
                     DYAGWQKYGYPVVSENTTAWINDILLQSETIGEEILEDERRDAFGMPLGNINEPMPSV
                     KLPGFDVTLRAMFREQYCQERYGTFDDDSFPIAKQSRAEVKRALEFISTSDKEDITWK
                     RVNSKEIVFVYPSKLSVIFLKFASLLGPSSGKNGEHSKARFEKSSEDFIKTFRGLEPQ
                     NKPDNIRIFSIRKMDKARSKVVFTRNLSPEWYIDCAEIWQAGCKNVPRINFTKSYTPF
                     PLEVADIINVVWKQNGELATQGKTAVKQIQYYQGMELLIDDESESAIRHYLNILLNNS
                     QGLFAFYGNQSSRMARAKDAQRHFPNAQKSIANLWPLFGLLLYKSGCKKEDYMENAAY
                     LVGQLLKVSDELHAMYCRVKREGDIPSQLAGNSVFVFASETPVKALAVLCQRMMPYIA
                     WAKQYRTKGVKYDPNDERLKENKGKSSGLAKYYLSLYESVASKLKSTLTLNIRFDDFE
                     KAQVFLGYLAALPKEERQNDAETFVEAENTTEEEYKNE"
     gene            6075..>6595
                     /locus_tag="PLL88_10025"
     CDS             6075..>6595
                     /locus_tag="PLL88_10025"
                     /inference="COORDINATES: protein motif:HMM:NF016963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HOJ01943.1"
                     /translation="MNNEIKRATGLLVIEVVNSNPNGDPDRESDPRQRPNGLGEISPV
                     SFKRKLRDLLEDKNSPFFKSLPKIYKEHPESYCILESRGRNRKAIQTEMGKDTSKFEQ
                     KDFLDSEFVKKYWDARVFGNTFLEEGANKGYIKTGVVQFGVGVSLSPISVIRHTNTNK
                     SGVQEGKNAGMAP"
BASE COUNT         1815 a         1448 c         1583 g         1749 t
ORIGIN      
        1 ctcgtacttt gctcgtatca gagctatcaa gcatcgagcc tttgcatgaa gacatccttg
       61 tagacgggaa gctcgtttac gattttccat cccttcaaga aattcgctcc atccgtgaaa
      121 aagatatggc agatctctac cccggagtaa aacggcttgt catgccacac aattaccacg
      181 tttccctttc acagaagtta tgggatctga aacacaccct ggtgctaaaa gcaacccatg
      241 aggatcaatg acgtttacga aggttggaat ataagtggaa gtctttgaga tcatccaccc
      301 cgattttaaa tctctggtag atataaaacg tcatccggag atcatccaga gtgaatttca
      361 attcaccgaa ggtcctgttt ggcacgcaca acagcactgt ctgtattttt ccgatatacc
      421 agcaaatacg ttgtattgct actcagaaca aaagggggtt gaaatcgctt tccaacccag
      481 tggattcagc aacggtctaa cgctcgatcc ttctggggca attctggctt gcgaacatcg
      541 cacccgggct attaccagat atgctgaaag cgaaaaaaca attcttgctg atacttatca
      601 agggaaaaaa ctgaactctc ccaatgacat cgtcgttaca aagcaggggc tcatctattt
      661 caccgatccc acctatggat tgcgagaagg gaacggaggt ccggcagaag cagaattacc
      721 tttccagggt ctttactgtt accggactga ttgggcagag ccaaagctgg tttgtgctga
      781 ttttgaacgc cccaacgggg tagcgctttc caatgatcag aagaccctgt tcgtaatcga
      841 caccgtaaaa caacacatcc gttcattcca aatccaagaa gatggcagcc tggtggacgg
      901 tcgggtaatt ctcgaattat ggggaaaagg acctgacaga ccggatgggt tgaaactgga
      961 tgagtttgat aacatcttca gtaccggtcc cgaaggcatt tgggtctttc ggtctgatca
     1021 tgcgctgctg ggtaagatac ggtttccgca gaaaacagca aacctcgcct ttggaggcga
     1081 ggatcgcagt tcattgttca ttacgtccag ccagtatctg atacggctgc gtttgaagac
     1141 caggggttta acaccgctgg atctttcaaa gtgatcctat ttcctgtcgc gatagaccac
     1201 atcttcaatc cattcctgtg attgcagcgg gtcgacgttc cttttttctt ttttattgaa
     1261 gcggtcatga agaaatgaca agaaagatgg gctttgcttg ttttgtgtct ctacctttgt
     1321 atcttcagat tcttcttgac gcccgattct ctcagcgcgg aactgtggaa ctgggatgtt
     1381 ttgagcgatg gcaggagcag atggctgttg gaaggctttt tcttccatga aaggttgaga
     1441 cggaacagct atcccttcac catctagcat ttcagtgctc ttggattgac tggttttcgg
     1501 caagaaacgg gtctgcagat catccaatga gcccacgaac cagttcagat caaccgatgc
     1561 tttgatgccg ggcacgcgtc ccttgtccgt atattgccag aaatcccagg cgctccatgg
     1621 gtagatcgtg cctggccatt ggctggtgta gcgtgccatc cacaacggat attccaccgc
     1681 ccaatcgacg tcggtcaatt tttcgtaggt catgtagggt cgccagaatc cgccactggt
     1741 ataaatgatc ggtttgcgac cggttatgct ggttatcgtc tcgacaaaac tctgtacttt
     1801 gcgaaccagt ttggagccct tgctcccggc tacctccaga tcaagcacca atggcaggct
     1861 gggcacgctg cctaccagct tgcaaaatga ttcagcctga atgattgagt caatgtgggt
     1921 gcgaaagaaa tgataagctc cccaatagat accatttgcc tctgcgccct ttcggtttat
     1981 ctcaaatttg ttatccacga acatcgtggt ctttttatca ggaaattccg atgccttgat
     2041 aaaggcaaat ttcacgccag ccgcttttac tttcgcccaa tcgatatcgc cctgccagtg
     2101 tgagacgtca attccaggta tcatttctca cctctttacc aatccttcat ttgcgagcgc
     2161 ctgcaggtct gcctgacttc cgttgaacca attcaaaccc ggtgtaccgc tcacgctggg
     2221 aatcttgacc tggtacgaga attgccatat cgcccaccct gcccatggaa acaactgggc
     2281 aggccagatg acacccggct catcgatcca cagcggatat tcacagggcc actcaattcc
     2341 tgccatgtac ttcttccaat aggtcgcaga ggtatagaca agcggtcgtt ttccaatgcg
     2401 cgtctctgcg gtttcaagaa aagtgcgcag tttcggctcc agcaccgttt tggaagtgga
     2461 atacaactca aggcaaacca ccggcgggag atcatcatct acgattgctg catttttgat
     2521 aaagtgctcc gcttgcacaa ccgcatcctg tgccgggttc agccagtgat aagcacccgt
     2581 caggatcccg gtttgtttgg cgtttttctt gtttccggca taagttgcca cagttcccgc
     2641 aatcccatca gtgcatttca gatacacaaa acgcacctgg ttttttgcca cctcaaccca
     2701 ggaagtgacg ttattttttt ccgatatgtc tattcctatg atcatgccat taatagtgca
     2761 ggttttatgc cagcttgtgc ctggtggaat tcctgtatag gtattgttaa tattttttcg
     2821 gtataataaa ggtcgattcg gggatgatag gcttcgacgg aggaggctga acccggactg
     2881 cgagctgaga ggtaccgacc tcattaaatc agtacaaaca acaagtgcca aacgcacaaa
     2941 ctatgcaacc ctcccattag ctgcataagc tgatagttgg gttcatggtc tctcagtaga
     3001 ccacgtctgc ccatcccgtt ctccggtcgg agatggagca ggcgtcacaa gaccggagtg
     3061 ccgtctggat ggctgcgctc caggcgtaag cgggctttcg ggcccgagcg gctggttgca
     3121 gaactggttg ggtggttgac ctgtctgcga caagagtaat aatcacctac gctcgtagac
     3181 gttcgcggct ctattctttc ggacgcgggt tcgattcccg ccatctccac catatttata
     3241 aataggaggt cgcctatggt tcgattatcc tatccgattg aaatggagat ttccggaaat
     3301 accgccatgt ggacgcgccc ggatacaggc gattgtccgg tcagttatcc tgcgccgact
     3361 tattcggcag tcaaagcgat ttttgaatcc atcctttggg ggcctgcggt ggagattata
     3421 ccgattaagg ttgagatctg cacgccgatt cagtatcacg cgtatgttac caattacggc
     3481 ggcccgctcc ggaaaaatat cgcgatcaac aaaaacaata attatcaatt gcttgcaaca
     3541 gtcctcatcg atgtttgtta taaactgtat gcagaagtca gacctaataa agaaaaaagc
     3601 aaattgccgc agcgagcaaa gcaatgggat aacaaaacca cttcgccggg acatgcgtat
     3661 caagctatgt tcaaccggcg gttaaagtgt ggacaatgtt attcgattcc ggcgttagga
     3721 tggcgtgaat ttacgccttc gtattttggc tgcctgcggt ctgatacaga ggttttgtcg
     3781 gaacttgcgc ctgtttctat tccctctatg ctgcgcgaag tgttcccggg gggctatcat
     3841 tcctcagtat cgtttgtata tgaccaggat ttggtgatta agaacggctg tttgacttat
     3901 ccagtcaaag gaggtagcca tgctgaatga acttcatagt ttatcggacg ctttaagtgg
     3961 catgtccatt cctactaaag aatggcatcg agaatacaaa ttaattccaa aagttactgc
     4021 gaaagctcca tgtattcgca tttggattgg tgaggatggc tcaatttacg ggtttgacaa
     4081 tatagatgca gaactttcaa tggtgattcg aaaatatggc aacaatcaaa actcttttcc
     4141 tgcgtttaat gtggcgccgc tttatcgtat cacagataag caaataatca aggaactgga
     4201 gcaaacgact aaagatcatg ctttactcga ttttgatgaa ataaaatcgt ggtgcacaga
     4261 agataactgg cgtatgagca tcgttcaaaa ggtgaataac tgcataagca aaagtgcgtt
     4321 agaattgcaa gaatatatta gacagcaggc tcctgcacag gagaattcga taaccgcgct
     4381 aattagcagc ctgcaagttt attctgatac ggatgatggc ggcaaagttt ttcgagaaaa
     4441 gttggaggct tgcatatttt ccaaattacg ggtaggagag agcattgaag ctgcgcttgc
     4501 catattactt cataaaggaa attcggaggc aaaagatccg gaaaaggata gcggctccct
     4561 gtcggtgatc ttggattatg ccggatggca aaaatatgga tatcctgtgg taagcgaaaa
     4621 tacgaccgcg tggataaatg atatccttct tcaatcggaa acaatagggg aagagattct
     4681 agaagatgag agaagagacg cttttggaat gccacttgga aatattaatg aaccaatgcc
     4741 gagcgtgaaa cttcctggtt ttgacgtgac attgcgcgcc atgtttcgtg agcaatattg
     4801 ccaagaacga tatggtactt tcgatgatga ttcatttccg attgcaaagc aaagccgtgc
     4861 tgaagtaaag cgagctttgg aatttatctc tacatcagat aaagaggaca taacatggaa
     4921 gagagttaat agcaaagaga ttgtttttgt ctatccatca aaacttagtg ttatattttt
     4981 gaagtttgcg tctttgcttg gcccatcatc cggtaagaat ggagagcatt caaaagcgag
     5041 gtttgaaaag agttcagaag attttataaa aacctttcgt ggacttgaac cacagaacaa
     5101 accagacaac atccggattt tctcaattcg gaagatggac aaggctcgct caaaggtcgt
     5161 gtttacccgt aatctttcgc cggaatggta tatagattgt gcagaaatat ggcaagccgg
     5221 ttgtaaaaat gttccaagga tcaatttcac caagtcatat acgccttttc cattggaggt
     5281 tgcggatatt atcaatgtcg tttggaaaca aaatggtgag cttgcaacgc aaggaaaaac
     5341 agctgtcaaa cagatacagt attatcaagg aatggaacta ctcattgacg atgaaagtga
     5401 gtccgcgata cgtcattatt tgaatattct tctcaataat agtcaggggt tgttcgcttt
     5461 ttatgggaac cagagctctc gaatggcgcg ggcaaaagac gctcaaaggc attttccgaa
     5521 tgcccaaaaa tcaattgcta atctttggcc gctgtttgga ttgttgcttt ataaaagcgg
     5581 atgtaaaaag gaggattata tggaaaacgc tgcttatctg gttggacaac tattgaaagt
     5641 ttccgatgag ctgcatgcaa tgtattgtcg agtgaaacga gagggagata tcccatcgca
     5701 gcttgcagga aactccgtat ttgtcttcgc atcggaaacg ccggttaaag cgcttgcggt
     5761 gttgtgccaa aggatgatgc catatattgc gtgggcaaaa cagtatcgta ctaagggcgt
     5821 aaagtatgat ccaaacgatg aaaggctaaa agaaaacaaa ggtaagagca gtggtcttgc
     5881 gaaatactat ctgagtctgt acgaaagcgt tgcttcaaaa ctgaaaagta cgttaacgct
     5941 taacattcga tttgatgatt ttgaaaaggc gcaggttttt ctaggttatc tcgctgcgct
     6001 tccaaaagaa gaaaggcaaa atgatgctga gacatttgtc gaagctgaga atactacaga
     6061 ggaggaatat aaaaatgaat aacgagataa agcgtgcaac agggcttttg gtcattgaag
     6121 ttgtcaattc caaccccaac ggggatcccg atcgggagag cgacccgcgc cagcgcccca
     6181 acggattagg cgaaatctct ccggtgtcct ttaagcgaaa actgcgcgat ttgcttgaag
     6241 ataaaaatag ccctttcttc aaaagtttgc caaagattta taaggagcat ccggaaagct
     6301 attgcatttt agaatctcgc gggcggaata gaaaagcgat tcagactgaa atgggaaaag
     6361 acacgtcaaa atttgagcaa aaagatttct tggacagcga atttgttaag aagtactggg
     6421 atgccagagt gttcggcaat acgtttttgg aagaaggagc aaacaaaggc tatattaaaa
     6481 ccggtgttgt gcagttcggc gtgggtgtgt ctttgtctcc catcagcgta attaggcaca
     6541 cgaatacaaa taaatccggc gtgcaagaag gaaagaacgc tggtatggct ccatt
//
LOCUS       DAOSDS010000109         6564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4238_length_6564_cov_5.423260, whole genome shotgun sequence.
ACCESSION   DAOSDS010000109 DAOSDS010000000
VERSION     DAOSDS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_6564_cov_5.423260"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PLL88_10030"
     CDS             complement(<1..537)
                     /locus_tag="PLL88_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01944.1"
                     /translation="MVILLVAPVVLATSILIAAILEIRSNVAFLLALYLFSISNIVIT
                     CTIAGLFHQLNNQSAYILIHFLFLIITLLCWQRHKKPAIQTPKQCFQSVGQFARQIFQ
                     SRELLVFFILIFISILFQLLLIYIMPPNTHDSMTTHLSRVGYWLQNNSYYPMNIHNQR
                     DDFYPVNPAFQALWTIVSF"
     gene            complement(793..1758)
                     /locus_tag="PLL88_10035"
     CDS             complement(793..1758)
                     /locus_tag="PLL88_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ01945.1"
                     /translation="MYTHKRQSAKVAVIIPAFNVENYITTVIRQLPQFIDDIVVVDDC
                     SRDSTAQQVEKIHDKRIHLIRHAQNQGVGGAMLSGYKLANELGAEIMVKIDGDDQMDP
                     AYIESLIIPIIELKADYTKGNRFLHMKELQRMPIIRRIGNLGLSFFTKLASGYWNIFD
                     PTNGYTAIHRTVYQLMNKEKIEQDYFFETSMLLQLNMINALILDIPIPAKYENQESNL
                     SPIKTLLVFPVKLIRGLINRVLYHYFLFDFSIVSIYLMIGIPSLLFGFIWGLAKWIHA
                     IRTGIVTTTGTVLIAVLPIIIGVQLFTQALSLDVNNIPTTSIRQE"
     gene            complement(1875..3275)
                     /locus_tag="PLL88_10040"
     CDS             complement(1875..3275)
                     /locus_tag="PLL88_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOJ01946.1"
                     /translation="MKKFFSNLMARMRAADKTVRLITIIFLILAIAAGVYLYGFVANF
                     VKEMTIVNLPGAPVLDQSQSSNGTPQPTAELLAQPTIEPWDGISRINVLFLGLDYRDW
                     EAGETPRSDTMILFSIDPSSSTASMLSIPRDLWVNIPDFGYYKINEAYFLGEGSHLPG
                     GGPQLAVTTVEEFLGVPIQYYAQVDFSAFISFIDEIGGIKITPTVDVKVEEFGSEYHQ
                     YLKAGEQYTLPGSLALSYARDRYTGDGDFGRASRQQQVIMAIIDRITQYNQLPTLIAR
                     APALYASLSSGIRTNMDLSTLVKVASLMINIPPASFQKAIIGTDMVIMTKSPSGLDIL
                     KPINDKIRELRDQLFTSGGSLGPLAGAASGSTLVRDEAATVLVQNSTQDATITSRTIA
                     YLQQQGINTVEAASSQLGMPTTIVVYHSKPHTVAYLASLMNVASTNIQFSYDPASAVD
                     IAILIGSDWVASATIP"
     gene            complement(3305..3532)
                     /locus_tag="PLL88_10045"
     CDS             complement(3305..3532)
                     /locus_tag="PLL88_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01947.1"
                     /translation="MISKDLLEILRCPVCVREEGKEGNLKVYKDAWLLCEDCDRKYPI
                     VEDIPVMLIDEGDKWAGKSKEDLPVPAPRPN"
     gene            complement(3596..5452)
                     /locus_tag="PLL88_10050"
     CDS             complement(3596..5452)
                     /locus_tag="PLL88_10050"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HOJ01948.1"
                     /translation="MVTSQKAFSLRSLITQGLLSILYAVLTFTLCAFLFFLGFNIWYA
                     GRIFPGIYFDTIALGSLSRQEAQQIIQTQVIPALPQSITLTYQDQTWNTNLLAMGISY
                     DPQQIADFAFSIGRSGSPAIWLGDVVALLSGNKSIPPAILFNEAAAAETLSEIAQELD
                     RPVEEARISFESSNVTTTPGQIGRTLDIEQSLEVVREALRQQSLAIIPLRVSEAAPDI
                     LDAEPYAQTIREFLASDFVLVTPEEYAHPSAEQPLTASVLSPMLELVKKNQDGQISIT
                     LQFKESVLQNLLDDLASKFNVDTANPRFIFNDSSGEIELYANGIIGRTLNVAASVSNI
                     QQQIASGAHEAVLVFDYTAPRVADTASGTELGITELVAHQRTYFYGSSSARVQNIKAA
                     AGRFLGILVAPGEVFSMAQHLGEISLDTGFTEALIIYNGKTIEGVGGGVCQVSTTLFR
                     TAFFGGYPILERYPHAYRVSYYEQIYNGRVDTNLAGLDATVYVPLVDLKFQNDSPYWL
                     LMEAYVYPESSQIEWKFYSTSDGRSVEWKTSGPTNIVEPPETKYYLNTQLSRGEIKQV
                     DWEAEGSDVRVERWVYKNGALYFQDQFLTHYEPWGAVYEIGPGVDGYPISEE"
     gene            5861..>6564
                     /locus_tag="PLL88_10055"
     rRNA            5861..>6564
                     /locus_tag="PLL88_10055"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1662 a         1454 c         1769 g         1679 t
ORIGIN      
        1 aaaggaaacg atcgtccaaa gagcctggaa tgccggattg accgggtaaa aatcatcccg
       61 ttgattatga atgttcattg ggtagtaaga gttgttctgc agccaatagc caacgcgcga
      121 cagatgggtg gtcatgctgt catgggtgtt tgggggcatg atatagatta aaagaagttg
      181 gaaaaggata gaaatgaaga tgaggatgaa gaaaaccaac aattcgcgag attgaaagat
      241 ctgtctggca aattgcccga cggattggaa gcattgtttc ggtgtttgta ttgcgggctt
      301 tttgtgtctt tgccagcaca gcagggtaat gataagaaaa aggaaatgga taagaatata
      361 tgcagactga ttatttaatt ggtggaagag tccggcaatg gtgcaggtta ttacgatgtt
      421 tgaaatggaa aagaggtaga gtgccagtaa gaaggcaacg ttcgatctta tttcgaggat
      481 cgctgcgatc aggatcgatg ttgccagtac aacgggcgct actaaaagaa tgaccataac
      541 cccttctata atgaattttt gataatccta tcgaacgctt ttgttctatg aacaggaaga
      601 tcaaattcaa gccaatgata ccattttaca caacgctgat tggaaaaatt tcgatgacgt
      661 cacaggaaac cgaaagaagt tctgaagaag caagtggcat tacaagatca tgtgcaattt
      721 caatttatgt gcctggtaat tagtgattaa gagctgcttt ccggtgacga ttgaagaaac
      781 ttatccaggt ctttattctt gtctgattga ggtcgtgggg atgttgttga catcgagtga
      841 aagggcttgg gtgaagagct gcaccccgat gatgatcggt aaaacagcga tcaatactgt
      901 cccggttgtg gttacgatac ctgttcggat ggcgtggatc cattttgcca atccccaaat
      961 gaatccaaag agcagtgaag gaattcctat catcaaataa atcgatacga tggaaaaatc
     1021 gaataaaaag taatgataga gcacacgatt gatcaacccc cgaatcaatt tgacgggaaa
     1081 aaccaacagt gtttttatcg gtgataaatt actctcttgg ttctcatatt tcgctggaat
     1141 tggaatgtcc agtatcagag cgttaatcat attgagttgt aataacatac tggtttcaaa
     1201 aaaataatcc tgctcgattt tttctttgtt catcagttga tagacggtcc tgtgaatggc
     1261 agtgtagccg ttggtcggat cgaaaatatt ccagtatccg cttgccagtt tggtgaagaa
     1321 tgataagccc agatttccga tgcggcggat gatcggcatc ctttgcaatt ctttcatatg
     1381 taggaagcgg ttgccttttg tgtaatcggc tttcagttcg atgatcggga tgatgaggga
     1441 ctcaatataa gcggggtcca tttgatcatc gccatcaatt ttgaccatga tctccgcgcc
     1501 aagctcgttg gcaagtttgt atcctgaaag catcgcaccg cccactccct ggttctgagc
     1561 atgccgtatg agatggatgc gcttatcgtg gattttctca acctgctgag cggtggaatc
     1621 gcggctgcag tcgtccacca ccacgatatc gtcgatgaac tggggtaact gcctgatcac
     1681 cgtggtgatg tagttttcta cattgaaagc cgggataatc accgcgactt tcgcgctttg
     1741 ccttttgtgt gtgtacaatt gcagctcctt tggaatgcca accagtgatc acttataaaa
     1801 gaagtatatg caagaaaaat gaaaatcgcc agacgaattt ctggcgattt ggttagattt
     1861 aggggtttgt aggtttaggg gatggtggca gatgcaaccc agtcgctgcc aatgagaatg
     1921 gctatgtcaa ccgcagaggc tgggtcatag ctgaactgga tattggtgga tgccacgttc
     1981 atcagactgg ctaaatacgc cacggtatgc ggcttgctgt gataaacgac gatggtggtc
     2041 ggcatgccaa gctgactgct ggcagcttca acagtgttga tgccctgttg ctgcaagtag
     2101 gcgatggtgc ggcttgtgat ggtggcatcc tgcgtactgt tttgaaccag caccgtggct
     2161 gcttcatctc ttaccagggt ggatccgctc gcagcgccag ccagaggtcc caacgaacct
     2221 ccggaagtga acaattgatc gcgcagttca cggatcttgt cgttgatggg cttcagaata
     2281 tccagcccac tcggagattt ggtcatgatg accatgtcgg tgccaatgat cgctttttga
     2341 aaactggcag gtggaatgtt gatcatcaga gatgcgacct tgaccagtgt ggatagatcc
     2401 atattggtac ggattccgga tgataagctg gcgtacaacg cgggcgccct ggcgatcaat
     2461 gttggcaact ggttatattg ggtgatgcgg tcgatgatcg ccatgatgac ctgctgctgc
     2521 ctgctggcgc gtccaaaatc gccatcgccg gtatagcggt cgcgggcgta agaaagtgcc
     2581 agagagcccg gcagggtata ttgttctccg gcttttaaat attggtgata ttcagatccg
     2641 aactcttcca ctttaacgtc aacggtcggg gtgatcttaa taccaccaat ttcgtcaatg
     2701 aatgaaataa aggcggagaa atcaacctgg gcgtaatatt ggatcggtac gcccaaaaat
     2761 tcttccacgg tcgtcacagc cagttgcggt ccgccacccg gcaggtggct gccttcgcca
     2821 agaaaatagg cttcattgat cttgtagtat ccaaaatcgg gaatattgac ccacaggtcg
     2881 cgcggaatgg aaagcatgct ggctgtactg ctggatgggt cgatcgaaaa gaggatcatg
     2941 gtgtcgctgc gcggggtttc ccctgcttcc caatcgcggt aatccaaccc taagaaaagc
     3001 acgttgatac ggcttatccc atcccacggt tctatggtgg gttgggcgag caattcagcc
     3061 gtgggttgtg gcgtaccatt gctgctttgt gattgatcca gaacgggcgc cccgggcaga
     3121 ttgacaatgg tcatttcttt gacaaaattt gcaacgaacc catatagata aacgcctgcc
     3181 gcgatcgcca gaatcagaaa gatgatggtg atgaggcgca cggttttgtc agcagcgcgc
     3241 atccttgcca ttaaattcga aaagaatttt ttcatgatat tttgttaact cgcaatactc
     3301 tcgatcaatt cgggcgtggt gctggcacgg gcagatcttc tttgctcttt ccagcccact
     3361 tgtcaccttc gtcgatcagc atgacgggaa tatcctcaac gatgggatat ttccgatcac
     3421 aatcttcgca cagcagccag gcatctttat acaccttgag gttgccctct ttcccctctt
     3481 cacgtacgca aacggggcag cgcaggattt ctaaaagatc tttactgatc atgcggtctc
     3541 ctttttgtaa aaagctcttt cggtatggtc agaattttac cactgcgtgc caggctcatt
     3601 cttcggaaat tgggtagcca tccacccctg gtccgatctc atagaccgct ccccagggtt
     3661 cataatgggt caggaattgg tcctgaaagt aaagcgcccc atttttatac acccagcgct
     3721 ccacccgcac gtctgaccct tcggcttccc agtccacctg tttgatctca cctcgcgaca
     3781 gttgcgtgtt cagataatat ttggtttcgg gcggctccac gatgttggtc ggaccgcttg
     3841 ttttccattc cacgctgcgc ccgtcgctgg tggaataaaa tttccactcg atctggctcg
     3901 actccgggta tacgtatgct tccattaata accaataggg tgaatcattt tggaatttca
     3961 aatcgaccaa cggcacatat acggtggcgt ccagaccagc caggtttgtg tcaaccctcc
     4021 cattgtagat ttgttcataa taagaaaccc tgtaggcgtg aggatatcgc tcgaggatgg
     4081 gatatccgcc gaagaaagcg gtgcggaaca gggtcgtgct tacctggcaa acgcccccgc
     4141 ccacgccttc gatcgtcttt ccattgtaaa tgatgagcgc ttccgtaaag ccggtatcca
     4201 ggctgatctc acccaggtgt tgtgccatag agaagacctc gccgggtgcg accaaaatgc
     4261 ccagaaaacg accagcggca gctttgatgt tttggacgcg cgcactggaa gaaccgtaaa
     4321 aataagtgcg ctggtgggca accagctcag tgatccccag ttcggtgccg ctggcggtat
     4381 cggcgactct cggtgcggtg tagtcaaaaa ccagcactgc ctcatgggct ccgctggcaa
     4441 tttgttgctg gatgttcgaa acgctggcag cgacgttcag cgttctgccg atgattccat
     4501 tcgcatatag ttcaatttct ccgctcgaat cgttgaagat gaagcgcggg tttgccgtat
     4561 cgacattgaa cttgcttgcc agatcgtcta aaaggttttg aaggacagac tctttgaact
     4621 gaagggtgat cgaaatttgc ccatcttggt ttttcttcac caattcaagc atgggtgaga
     4681 gaacgctggc ggtaaggggc tgttctgcgc ttggatgcgc gtattcttct ggtgtgacaa
     4741 gaacgaagtc agacgccaaa aactcgcgga tcgtttgggc gtaaggctcg gcgtccagaa
     4801 tatcgggtgc tgcttcgctt accctcagag ggatgatcgc aagactttgc tggcgcaagg
     4861 cttccctgac tacttccaag gactgttcaa tgtcgagcgt tctcccgatc tgcccgggag
     4921 tggtcgtaac gttgctgctc tcaaaggaga tgcgcgcctc ttctacaggg cggtctagct
     4981 cttgcgcgat ttcggacagc gtttctgctg ctgcagcttc attaaataga attgcgggag
     5041 gaatgctttt gtttccactg agcagagcta ccacgtcccc caaccagatg gcaggcgaac
     5101 cggagcgtcc gatggagaat gcgaaatcag cgatctgctg cggatcatag ctgatgccca
     5161 ttgccagcag attggtgttc caggtttgat cttgataggt gagggtaatg ctctgaggca
     5221 aggcggggat cacctgggtt tgaatgatct gttgtgcttc ctgcctgctc aagctaccca
     5281 aagcgatggt atcaaagtaa attcccggga aaatgcgccc ggcgtaccag atgttgaagc
     5341 caagaaagaa aaggaaagcg cagagggtga aggtgagcac cgcatacagg atggataata
     5401 aaccctgggt gatcaatgat cgaagggaga aagctttctg cgaagtaacc atttttgaat
     5461 tatagcaggg ttgaaaatgg cttttttaga agaaaacgct ggcaaaagcg cagtttttta
     5521 aggtttcctc gcttaaattc cttgacaggc ggtgaggaag ccgttacaat cgtctctacc
     5581 atttgacctt gtagatttgt aaacgcaaat catttacaac taaatagctg caagattgaa
     5641 ggcttgcagg gacggtgtaa gcagcgttac cggtgcgctc caaagcaaca ggaaagcatc
     5701 ggtaatttta tcccagggtc ttgacaggtg ctggagatgt ggttacaatt ctgtcccgca
     5761 atatggatgg gaaccttaac aactgaagag tgaaaggaaa gtgaaaccaa aattctattg
     5821 agtcaagaga actcaacacc aacttggttg gtgttcgatt tttgcggaga gtttgatcct
     5881 ggctcaggat gaacgctggc ggcgtgctta atacatgcaa gtcgaacgaa gctccttgtc
     5941 ttcggattag gagacttagt ggcgaacggg tgcgtaacgc gttggtaacc tgccccaaag
     6001 cgggggataa cagtccgaaa ggactgctaa taccccatgt ggtcatcagg gttagatacc
     6061 tgatgattaa aggagtaatc cactttggga ggggcctgcg tcccatcagc tagttggtag
     6121 ggtaatggcc taccaaggcg atgacgggta ggggacctga gagggtggcc ccccacactg
     6181 gcactgaaat acgggccaga cacctacggg tggcagcagt agggaatatt ggacaatggg
     6241 cgaaagcctg atccagcaac gccgcgtgca cgatgacggt cttcggattg taaagtgctt
     6301 ttaagggaga tgagaaagga cagtatccct tgaataagcc tcggcgaact acgtgccagc
     6361 agccgcggta aaacgtagga ggcgagcgtt atccggattt attgggcgta aagcgcgtgc
     6421 aggctgcttg gaaagttgga tgtgaaatct cctggcttaa ctgggagggg tcgttcaaaa
     6481 ctaccaagct agagaatggg agagggaagt ggaattccgg gtgtagtggt gaaatgcgta
     6541 gatatccgga ggaacaccag tggc
//
LOCUS       DAOSDS010000110         6557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4250_length_6557_cov_4.121040, whole genome shotgun sequence.
ACCESSION   DAOSDS010000110 DAOSDS010000000
VERSION     DAOSDS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4250_length_6557_cov_4.121040"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..1390
                     /locus_tag="PLL88_10060"
     CDS             167..1390
                     /locus_tag="PLL88_10060"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOJ01949.1"
                     /translation="MNTLLDHLKSLGVELGKEKKDYKKRELEDLTGILGGTWLETPYG
                     RTFHSQQIFDLQAAQGDVLLKCTRIGQKVLDLFNISHVLENSALLPTQICFFDTETSS
                     LSLGSGAFVFLTGFSHFQEDGSVCVDQFFLPHPSEERAFIYAVQEFLGHFSILSSYNG
                     KSFDVPMLRSRFIFHDMEDDGLEKTHLDLLFLARALWKRRLESCRLVEIEEAILHFKR
                     TGEDVPGWLVPILYQDYLREGHAAPLKSVFYHNTQDVVSLAALFNVIHAMLDQEMQYA
                     IEFPEDYASLAYLYEKLGNREQAETYFDAYFSSLAGSADPEVLYDYAWMQRRNENWSV
                     AVKYWHLAAEQDHLPAMVELAKYYEHQVKNVQQAMQWLERIKQYDPVKRSLLKDDLRK
                     RALRLERKSNKNVEN"
     gene            1377..1628
                     /locus_tag="PLL88_10065"
     CDS             1377..1628
                     /locus_tag="PLL88_10065"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOJ01950.1"
                     /translation="MSKIDDFESVYSASGRLDGEMMKNLLESFEIQCVLLGESVGSTY
                     GLTVTPVGKVEVMVHSADVERAEQIIQEYQNGLLEEKES"
     gene            complement(1625..2206)
                     /locus_tag="PLL88_10070"
     CDS             complement(1625..2206)
                     /locus_tag="PLL88_10070"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOJ01951.1"
                     /translation="MNCKQIAAIILAAGGSIRLGTPKQLIPWQGMTLLNFTIATIKES
                     GIEEVVIVLGASADVIRTSLKDTQLRIVVNAGWQTGKASSIRAGLNAIPGTAKGVLIF
                     LCDQPYLSAELIHKIIQAGEESEAKIIAPAVGEQLSNPVLFKKEIFSAFYTLQGEEGG
                     KNLFTRYPVQKVPWKDKRILLDIDTQDDLGKLA"
     gene            complement(2196..4409)
                     /locus_tag="PLL88_10075"
     CDS             complement(2196..4409)
                     /locus_tag="PLL88_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOJ01952.1"
                     /translation="MTDLIGKSVPRFDSLDKVTGHAKYPSDFAFQNEAYMQVVFAERP
                     HAVIKKIDVTKAQAFPGVLAVLTAGDVPCNEYGLIMNDQPVLCGPGSTKQGADHVRFY
                     GDQVALVIAENEEIARNASKLIKIDFEDLPIIEDPREAMKPGAFQLHADKEQNVILHY
                     KIRKGNVEEGFQDADVIIESDYATPLQEHAYLQPEAGVSYIDEKGRITVVVAGQWVHE
                     DQEQIAHALNLPVEKVRVIYPAIGGAFGGREDMSVQIILALAAWHLHQRGIQRPVKTV
                     WSREESFIGHHKRHPYYIHTRWGATKKGKITAVETTLIADAGAYAYTSTKVQGNATLL
                     SSGPYFVPNVKIDSFSVYTNNIPNGAFRGFGGPQACYAVEMQMSKLAEALKMDPVEFR
                     LLNAIQEEQPTAVRTPLPKGISIEKVIQECAVAAGWQKNGAGWKLQDGKKAGDADTIR
                     GIGFAAGYKNVGFSFGAPEHCWATVELHGKNEIEKVILHHAGADVGQGAHSVFRQFAA
                     SALKVDLEKVELVASDTAQTDNSGSASASRTTFMAGNAILGATKAALEKWDAEERPAI
                     ATYKYVPPATSPYDHETGECYPNFAYGYVAEAVEVEIRKSTGEITYKRVICADDVGKA
                     INPLQVKGQIEGAVVQAVGYASLEKFVQQKGLVKTGGLSTYLVPTIKDIPEETMSLIV
                     ETADPLGPNGARGMGEMPYLPFAAAVCDAVHAATGIWFNEFPLTQEVILQGLGEL"
     gene            complement(4419..5849)
                     /locus_tag="PLL88_10080"
     CDS             complement(4419..5849)
                     /locus_tag="PLL88_10080"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOJ01953.1"
                     /translation="MWNKYWTVTSVEEALEILANEKGKARIIAGGTDLMLELKHELHP
                     QVETLIDISRLHVSNPIKQDRDGTIHIGPNATHNHCIHSKIIRERAFPLAQAAYSVGA
                     PQIRNIGTIYGNLITASPANDTITPLVAMDATLEIRSKNSRRIVPLRDFYSGVRKIDL
                     KADEMVTDLYFQPLKENHHGIFSKFMLRKVHAISVVNVAVILTLDNGIIARAVVTLGA
                     VAPTIIHAKQCEEYLKGKPLTLTAEVIEKATELAVKDASPITDIRSSDQYRNRLVSVL
                     TKSALKAIAAGEERKGFPEKPVLLWGKKPYQPSTLDAITLHSATTPIKAIVNGREEQV
                     NGFQNQTLLKFIREGVGLTGSKLGCGEGECGACTMHLDGVPVLACLIPAPRAHGAYLK
                     TIEGVAPKTGLHPIQQAYVDEGAVQCGFCTPGFIMSSLALLEEFPHPTKEEIEIGLAG
                     NICRCTGYYSIINAVEKAARQMAHKG"
     gene            complement(5924..>6557)
                     /locus_tag="PLL88_10085"
     CDS             complement(5924..>6557)
                     /locus_tag="PLL88_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOJ01954.1"
                     /translation="SSNSGSTSASRMTYMAGHALQAAAKKALHEWQKEERPAVGEETY
                     HSPPTTELDEETGKGYPNFGVGYAAEAIRVRVDRETGEIDVLDMVCADDVGKAINPQQ
                     VRGQMEGCLIQALGYTQVEEFMQKNGILQTKNLSTYLIPTIRDIPQNIKSIIVEVPDP
                     HGPHGAKGMGEIPYGPFAPAFCAAVHAATGVWFNSLPLKPEKVVFNILND"
BASE COUNT         1512 a         1659 c         1572 g         1814 t
ORIGIN      
        1 gctggggttg cccgtcgtgc gtaggagcgg ttaatcagga gtttctcaac ccaaaagaag
       61 ccaccctggc gttgctgcag gagattctga aataaaagga ttctctctgt aacatttcaa
      121 catatagaat gcgatgattt ttattgtaaa atagtataaa agttctatga atactttact
      181 agatcacttg aaatcgctgg gcgtggagct ggggaaagag aaaaaagact ataaaaagcg
      241 tgagctggaa gatttgaccg gcatcctggg tggaacctgg ttagaaacac cctacggaag
      301 gacattccac agccagcaaa tattcgatct gcaggcagct caaggggatg tcctcttaaa
      361 gtgcacaaga attggacaga aggtcttgga cctgttcaat atttcgcacg ttttggaaaa
      421 ttctgccctc ctgcctaccc agatttgttt ttttgacacg gaaaccagca gcttgagcct
      481 aggcagcggt gcttttgtgt tcttgaccgg attcagccac tttcaggaag atggcagtgt
      541 gtgtgtggat caattcttcc tccctcaccc atccgaagaa cgggctttta tttacgccgt
      601 tcaggaattt ttggggcatt tttccatcct ttcttcctac aacgggaaga gctttgatgt
      661 gcccatgctg cgttctcgct tcattttcca tgatatggag gatgacgggc tggaaaaaac
      721 gcatttggac ttgcttttcc tggctcgcgc gctttggaaa aggcgcttgg aatcttgcag
      781 attggtggaa atagaagaag ccattcttca tttcaaaagg acaggagaag atgtgccggg
      841 ctggttggtc cccattcttt accaggacta cctgcgcgaa gggcatgcag cgcctttaaa
      901 gagcgttttc taccataaca cacaagatgt tgtttctctg gcagctctgt tcaatgtaat
      961 tcacgccatg ttggatcaag agatgcaata tgccatcgaa tttcctgaag attatgcttc
     1021 tctagcctat ctatatgaaa aactgggcaa cagagagcaa gccgaaacat actttgatgc
     1081 ctatttttca tcgctagcag gttctgctga cccggaagtg ttgtatgatt atgcgtggat
     1141 gcagcggcgc aacgaaaatt ggtctgtggc tgtgaaatac tggcatctgg cggcggagca
     1201 agatcacctg cccgccatgg tggagcttgc aaaatattat gagcatcagg tcaagaacgt
     1261 gcagcaagca atgcagtggc tggagaggat caaacaatac gacccggtga aacggtcgtt
     1321 gttaaaagat gatctgcgga aaagagcgct gcggctggag agaaagagta ataaaaatgt
     1381 cgaaaattga tgattttgag agtgtttatt cggcttccgg caggttggat ggtgaaatga
     1441 tgaaaaattt gctggaatcg ttcgagatcc agtgcgtgct gcttggagaa agtgttggtt
     1501 cgacatatgg tctgaccgtg accccggttg gaaaagtaga agtcatggta cattctgccg
     1561 atgtggaaag ggcagagcag atcattcagg aatatcaaaa cgggcttttg gaggaaaagg
     1621 agagctaagc cagttttccc agatcatcct gtgtgtcgat atccagcaaa attcgcttat
     1681 ctttccaagg tactttctgc accgggtacc gggtaaatag atttttccct ccctcttctc
     1741 cctgcaaggt ataaaacgca gaaaatatct cttttttgaa aagcaccgga ttgctcaact
     1801 gctcccccac tgccggagca atgatttttg cttctgattc ttccccagct tgaatgatct
     1861 tatggatcag ttctgccgaa aggtacggtt gatcacacag aaagatcagt acgcccttgg
     1921 cagtgcctgg aatggcgttc aatccggctc tgatcgagct ggctttgcca gtttgccagc
     1981 ccgcattaac aactatgcgc aattgtgtat cttttaacga tgttctgatc acgtcggcgc
     2041 tcgcacctag cacaatcacg acttcttcaa tcccgctctc ttttatggtc gcaatagtga
     2101 agttcagcaa ggtcatccct tgccagggaa ttaattgttt gggggtgcct aggcgaattg
     2161 atccgccggc tgccagtatg atggcagcga tctgcttaca gttcacctaa cccctgcaag
     2221 attacttcct gcgtcaatgg gaattcgttg aaccagattc cggtggcggc atgcactgcg
     2281 tcacaaaccg ccgctgcgaa gggcagatac ggcatttcgc ccattccgcg cgcaccattt
     2341 ggacccaacg ggtcggcagt ttcgactatt aacgacatcg tttcttcagg aatatccttg
     2401 atggtaggaa ccagataggt agatagacca ccggttttga ccaatccttt ctgctgcacg
     2461 aacttttcca ggctggcata tcccacggct tgtaccacag ctccttcaat ttgacctttc
     2521 acttgtaatg ggttgatcgc tttcccgaca tcatcagcac aaatgacccg tttatacgtt
     2581 atttccccgg tgcttttcct gatctccact tccaccgctt ctgccacata cccataggcg
     2641 aagttgggat aacattctcc agtttcatga tcgtacggac ttgtggcggg tgggacatat
     2701 ttatatgtag caatggcagg tcgttcttcc gcgtcccatt tttccagagc tgctttggtt
     2761 gctcccagga tggcatttcc tgccataaag gttgtgcgcg aagcggaagc gctgccagaa
     2821 ttgtcggtct gggcggtgtc cgatgcgacc agttcgactt tttccaggtc aaccttcaac
     2881 gctgaggcag cgaattgcct gaaaactgag tgcgcgccct gccccacatc tgcgccggca
     2941 tggtgcaaaa tgaccttttc gatctcattt tttccatgca gttcgaccgt cgcccagcaa
     3001 tgttctggcg cgccaaatga aaaaccgaca ttcttgtatc cggctgcaaa cccaatgcct
     3061 ctgatggtat cggcatcgcc agcttttttc ccatcctgca acttccaccc cgctccgttt
     3121 ttctgccatc cggcagctac cgcgcactcc tggatcacct tttcgatgct gatccctttg
     3181 ggtaatggcg tacgcaccgc agttggttgc tcttcctgaa tagcattcag caggcggaat
     3241 tccaccgggt ccatcttcag tgcttctgcc agtttgctca tttgcatttc taccgcataa
     3301 caagcctgcg gtccaccgaa accgcgaaaa gcgccgttgg ggatgttatt ggtataaacg
     3361 gaaaatgaat cgatcttcac attgggcaca aaataaggac cgcttgatag caaagtggca
     3421 ttcccctgca ccttggtgga agtataagcg tacgctcccg catctgcgat caaagtggtt
     3481 tccaccgcag taattttgcc ttttttggtt gctccccacc tggtgtgtat gtaatacgga
     3541 tgccgtttat gatgtccgat gaaggattct tctcggctcc agaccgtctt taccgggcgc
     3601 tggatgccgc gttggtgcag atgccaagca gccaatgcca ggatgatctg cacagacata
     3661 tcttccctcc cgccaaacgc accaccaatg gcagggtaaa tcactcgcac tttttcaacg
     3721 ggaagattga gcgcatgcgc gatctgctcc tggtcttcgt gcacccattg accagctact
     3781 acgaccgtga tacgcccttt ttcatcgata tagctgacac ctgcttctgg ctgcaggtaa
     3841 gcatgctctt gaagaggggt tgcataatca ctttcgatga tcacgtcagc gtcttgaaaa
     3901 ccttcctcga cgttcccctt tcgtatcttg taatgcagaa tgacattctg ttctttgtcc
     3961 gcgtggagct gaaaggcgcc cggtttcatc gcctctcgcg gatcctcaat gatcgggagg
     4021 tcttcaaaat ctattttgat cagcttgctg gcattgcgag cgatctcttc attttcggcg
     4081 atgaccaatg caacctgatc accataaaat cgcacatggt ccgcaccttg tttcgttgaa
     4141 ccaggtccgc ataaaaccgg ttggtcgttc atgatcagac catattcatt gcacggcaca
     4201 tcaccggcgg tcagcaccgc cagaacgcca gggaatgcct gtgctttggt aacgtcgatc
     4261 tttttaatga cggcatgcgg tcgttcggca aacaccacct gcatatatgc ttcattttga
     4321 aaagcaaaat cgcttggata tttcgcgtga ccggttacct tatcgagact gtcaaagcga
     4381 ggaacggatt tgccaatgag gtcagtcatc ttgtcctcct accccttatg cgccatttgt
     4441 cgcgcagctt tttcgactgc gttaatgatg ctgtaatatc cggtgcaacg gcagatgttt
     4501 cctgccagcc cgatttcgat ttcttctttt gttggatggg gaaattcttc aagcaatgcc
     4561 aggctggaca taatgaaacc tggcgtgcaa aaaccacact gtaccgctcc ttcatccaca
     4621 taagcttgtt gaatggggtg taaccctgtc tttggagcca ccccttcgat ggttttcaga
     4681 taagcgccgt gtgcgcgtgg cgcggggatc aaacacgcca acaccggaac tccgtccagg
     4741 tgcatcgtgc aagctccgca ttctccctct ccacatccca actttgagcc ggttaatccc
     4801 accccctcgc ggatgaattt caacagcgtc tgattttgga aaccattgac ttgttcctcg
     4861 cgcccgttga cgatcgcttt tatgggggtc gttgccgaat gcagggtaat tgcgtccagt
     4921 gtgcttggct gatacggctt cttgccccac aacagtaccg gcttttccgg aaaacctttc
     4981 cgctcctcac cggcagcgat cgcctttaat gcgcttttgg tcagcaccga taccaaacga
     5041 ttgcgatatt gatctgatga gcgaatgtct gtaatagggc tggcatcttt gactgcaagt
     5101 tcggtcgctt tttcaatgac ttcagcggtc aaggtcaacg gctttccttt gagatattcc
     5161 tcacattgct tggcatggat gatggtaggg gcaaccgcgc ccaaggtcac cactgcgcgg
     5221 gcaatgattc cattatccag tgtcagaatg acagcaacat tgaccacgga gatcgcatgc
     5281 accttccgca acatgaattt tgagaaaatc ccatggtgat tttccttcag cggttgaaaa
     5341 tagaggtctg tcaccatttc atccgctttc aggtcaatct ttctcacccc cgaataaaaa
     5401 tcgcgcagag gaacgattct tctcgaattt tttgagcgta tttcaagcgt ggcatccatg
     5461 gctaccaaag gagtaatggt atcgttggct ggagaggcgg tgataaggtt cccgtagatc
     5521 gtgcctatgt tccggatctg tggtgctccc acggaatagg ctgcctgcgc caatgggaaa
     5581 gccctttctc gtatgatctt ggaatggatg cagtgattat gcgtggcatt tggacctata
     5641 tggatggtcc catcccgatc ttgtttgatc ggattggaga cgtggaggcg gctgatatca
     5701 atcagggttt ctacttgcgg atgcagttcg tgttttaact ccagcattaa atctgttccc
     5761 ccagcaatga tgcgggcttt tcccttctcg tttgcgagaa tttcaagcgc ttcttctacc
     5821 gatgtgacag tccaatactt gttccacata ctacctccgc tttccatttt actctactgg
     5881 tagcacaatt ctattaattc tttaatttta tagtacgaag ctcctaatcg ttcaaaatat
     5941 tgaaaacgac tttttccggt tttaaaggca acgaattgaa ccaaacccct gtagctgcat
     6001 gtactgcggc gcaaaatgcc ggtgcaaaag gtccatacgg gatttctccc attcccttgg
     6061 ctccgtgcgg accatgggga tcgggaactt ctacaatgat gctctttata ttttgaggga
     6121 tatccctgat agttggaatc agataggttg aaagattctt cgtttgcaaa attccatttt
     6181 tctgcatgaa ctcttcgact tgtgtgtagc caagggcttg aatgaggcag ccctccatct
     6241 gcccccgcac ttgttgcgga ttgatggctt tcccaacatc gtctgcgcaa accatatcga
     6301 gaacatcgat ctctcctgtc tcccgatcca ccctcacccg gattgcttcg gctgcatatc
     6361 ccaccccgaa attcggatat ccttttccag tctcctcatc cagttccgtg gtgggaggag
     6421 aatgataggt ttcttcaccg acagccgggc gttcttcttt ttgccattca tgcaaggctt
     6481 ttttcgccgc cgcttgcaga gcgtgccctg ccatgtaggt catgcgcgat gcggatgtgc
     6541 tccctgaatt ggatgaa
//
LOCUS       DAOSDS010000111         6518 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4283_length_6518_cov_4.460931, whole genome shotgun sequence.
ACCESSION   DAOSDS010000111 DAOSDS010000000
VERSION     DAOSDS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4283_length_6518_cov_4.460931"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PLL88_10090"
     CDS             complement(<1..564)
                     /locus_tag="PLL88_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01955.1"
                     /translation="MYWLRESTYLGVLLWVACTLMWSIGGNWLVASIFSLHKHEIPSL
                     GFGSGLVLYLWLANILGHFLPSSLAFLLPAPMIFLTGYFAWRLNPQKENPFHLKTIFT
                     QALVFAALTGFFTLIGRGLAIFDERKNLSLISLMANGDIPPHNPLNPSTLYQYHYGSQ
                     LLGASMVRLGGFFPWSAFDISKAIYWAL"
     gene            complement(571..1548)
                     /locus_tag="PLL88_10095"
     CDS             complement(571..1548)
                     /locus_tag="PLL88_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOJ01956.1"
                     /translation="MKTETNANPLEPEEQPKKQSSYWRWLLTGIAIGLFIYQFSRQDF
                     QETFHIIKSMPMATIIGTLLLMTGSRIATAARWNALLKINDPKPKFKDTLKTTYAGLF
                     SSNILPTSIGGDFVRLLMGIQTELDPAYVASSLIMDRITGFAGMFLFMPFGLYLLFNM
                     DKSEAAFATSITSLVVMGTFSDVSTKLLRWLKKFIQKIWDSVKLWVRHPAFLLESLGF
                     TLLHMAFYFGAIWLLLHTMQVSISIFKIGAIYSLSYIISLFPLSIGGLGVQELAISNL
                     FSMLGNMSMETANALALLIRMAFIISSIPGVFFLPDIRKRKKQIAEKEK"
     gene            complement(1545..3536)
                     /locus_tag="PLL88_10100"
     CDS             complement(1545..3536)
                     /locus_tag="PLL88_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01957.1"
                     /translation="MKKFIQILEKSDLLQKLFILLFCLLSLAAFLEGTNNGLYETNSA
                     WQIIGYANGILACLGFTTLILTILKKDKGLKLLFKKIAAPFKRSRLLTWVLFGVFMGG
                     LLYCIFVRFDRYFSGPLLRAHTIYFAACLTAGMLKGAYREKPFNGLLLFSLLALGCAY
                     QAGNHLAEISSNPFTLEWSEGSRYYYSSLLFSEKLYGQQLPLSFLHPSRYVILSLPFL
                     LPNAGIFVHRAWQVFLWLSTTLICMIGMVKRLKQKDSWWNAILVLSGLLFLQQGPIYY
                     HLLISAILVLFGFDRNKFWKSCLWVILASCWAGISRINWYPLPGVLAALLYFFETPQK
                     EKTKTWEYIKEPFFYIVIGIFFSFIANMAYIPLSGNSTIENFTTSFTSDLLWYRLLPS
                     PTFPKGILTGILWLTAPLLLFLGFSLPRLREDFGWIQRTFLISALSVFFIGGLFVSVK
                     IGGGSNLHNMDAFLLLMLTSVNYLCFSDFYPNQKIAWTSAFSVLLTAALLLQPVFWAL
                     SAWSPRVYYSGESLQHDLAEINGAIDSINTHHPHSKILFINQRQLLTFGYVTATPLIS
                     DYELLELMEMAISGQSAYLQQFYADLAAHRFDLIIVNKQYIIFKDEEDAFPEENNAWV
                     KNITIPLMRYYTPITWLRYTDTEIYVPRAENELQRIVTQ"
     gene            complement(3511..5013)
                     /locus_tag="PLL88_10105"
     CDS             complement(3511..5013)
                     /locus_tag="PLL88_10105"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOJ01958.1"
                     /translation="MPSRTQRVLKETTIYGLGGAGSKFISFLLFPLYSRLFNVTEYGA
                     IEVINTFLALASILLTSGTDLAQSFYFYEYKGDSERKKTISTLSIYVFLINTLVALLV
                     GVFSKQISLWLLGSADFSNLLRIACLVIPWMGLYTFNLNLLRLQRKPIVYISITLPFI
                     LLQISANIIFVLLMQGGMASIFWTNLACYILFTVIGIYINRSFFSAHFDLPQFKEMFR
                     YWRPLIPVNVSAWFLASADRLILSSMSTLEATGIYSAGLRIASVVGFFVQAFRTANLP
                     FIFDVSKETDASTVYQKTLSYYLYFASLIAVAVSLFAKPILLFFSGREYLPAVQVIPP
                     LAFVYVLSGTSQIVSIGSLISKNTKNIGWITAISALLMGCGLLLFIPGHAERGTAYVV
                     LAANLIYNFALYFNSQKSHPIPYDWKRILRIVGVSSGIIFLSLLVQIEKLWLEILIGL
                     IGLCFFLLLTPVFSLLTTSEIKALQKQIQLFVHNLFRGKKPQHEKVHPDS"
     gene            complement(5028..5960)
                     /locus_tag="PLL88_10110"
     CDS             complement(5028..5960)
                     /locus_tag="PLL88_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ01959.1"
                     /translation="MRFLITGAAGFLGAPLANSLVSDGHSVVGLDDLSTGDPDRLSSD
                     VHLVRGDINDRPKLWTLLQGVDCVYHLAARVIVPESVLYPREYNLVNVGGTVTLMEAM
                     RDVGVKRVVFISSGSIYGNQHNQPMKETLRPQPRSPYAVSKLAAEYYIRTIGELWKIE
                     TVILRVFNAYGPEQRLPPAHAPVIPYCIKQALDNGTIVIHGEGKQTRDYVFMDDVVNA
                     AIRASSVSDVNRLIINVGSGRETSVNEIVRLVTELSGTKPEIVHNQHHDSGPSRMCAD
                     ISLAQKKLGYRPSVSLREGLLKTMQQDKRFHEKN"
     gene            6090..>6518
                     /locus_tag="PLL88_10115"
     CDS             6090..>6518
                     /locus_tag="PLL88_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01960.1"
                     /translation="MKKKAVFTLVLFTLLLGAHVGAVSAQGEGTPSSTPLAVEPVSTE
                     TPTATVNAGVSDKDLPLCLPGAYLETPEDCLPLGSSLILTELARKGFTIPLKPLAATK
                     PDTSLVDLDIKYAKLNLQAGMQAAFYPNLDSAVAGINAFRF"
BASE COUNT         1873 a         1441 c         1618 g         1586 t
ORIGIN      
        1 aagagcccag taaatggctt tactgatgtc gaatgcgctc caggggaaga atccgcccag
       61 cctgaccata ctggcaccca gcaattgtga accataatga tattggtaga gagttgaggg
      121 attcaacgga ttgtggggcg ggatatcccc attcgccatc agagagatca gtgatagatt
      181 ttttctttca tcgaaaatcg ccagtccgcg accaatgagg gtgaaaaatc ctgtcagtgc
      241 ggcaaagaca agcgcctgag tgaagatggt tttcaggtgg aatggatttt ctttttgggg
      301 attcaaacgc caggcgaagt agcctgtcaa aaagatcatt ggcgcgggta gcaggaacgc
      361 cagtgaactg ggcaggaaat gccctaaaat atttgccaac cacaggtata ggaccagtcc
      421 gcttccaaac cccagactgg ggatttcatg cttgtgcaga gaaaagatag aggcgaccag
      481 ccagttcccc cctatgctcc acatcagtgt gcaggcaacc catagtaata cgccaagata
      541 ggtgctttcg cgcagccagt acatacagga tcatttctcc ttttctgcga tttgcttttt
      601 cctcttgcga atatcaggaa ggaagaacac gccggggatg ctgctgatga tgaatgccat
      661 gcgaatcaat aatgccagcg cattggctgt ttccatagac atgtttccca acatcgagaa
      721 aaggttggaa atcgctaact cctgcactcc caatccgccg atcgacaggg gaaagagcga
      781 gataatataa ctgagactgt agatagcgcc gattttgaaa atggaaatag atacctgcat
      841 ggtgtgcagc aataaccaga ttgcgccaaa ataaaaagcc atgtgcagca aggtaaaccc
      901 aagcgattca agcagaaaag cgggatgcct tacccacagt tttacactgt cccatatctt
      961 ttggatgaac ttttttaacc atctcagcaa ctttgtagag acgtcagaga aggtacccat
     1021 aacgaccaac gacgtgatgg aggtcgcgaa agcagcctcg cttttgtcca tattgaagag
     1081 aagatacaat ccaaacggca taaacaagaa catgccggca aagccggtga tgcgatccat
     1141 gatcaaagaa gatgctacat aagccggatc cagttcagtt tgaatgccca tcaataagcg
     1201 cacaaagtca cccccgatgc tggtaggcaa aatgttcgag gaaaataacc ccgcataggt
     1261 agttttaaga gtgtctttga actttggttt gggatcgttt atttttaaca gggcattcca
     1321 gcgcgccgca gtggcaatcc gtgagccagt catcaacagc agagttccta tgatggtcgc
     1381 catcggcatg cttttaatga tgtgaaacgt ctcttgaaaa tcttggcgcg agaactgata
     1441 aatgaacaat ccgatagcga taccggtcag aagccacctc caataggagg attgtttttt
     1501 gggttgctct tctggctcta aaggattggc gtttgtttcc gttttcattg ggttactatc
     1561 ctctgcaatt cattttccgc gcgtggcacg tagatttcgg tgtcagtata acgcaaccag
     1621 gtgatcggtg tatagtatcg catcagtggg atggtgatgt tctttaccca ggcgttgttc
     1681 tcttctggaa atgcatcttc ttcatctttg aaaataatat attgcttgtt cacaatgatc
     1741 aggtcaaacc tgtgggctgc cagatctgcg tagaattgct gcaaataagc gctttgcccc
     1801 gaaatcgcca tttccatcaa ttccagcaat tcatagtcag atatcaaagg cgttgcggtc
     1861 acgtatccaa atgtcagtaa ctggcgttga ttgatgaata agatcttgct gtgtggatga
     1921 tgagtattga tcgaatcaat tgccccgttg atctcagcca gatcgtgctg caaagattcg
     1981 cctgaatagt aaacgcgtgg cgaccaggcg ctcaaagccc agaacacagg ctgcaaaagc
     2041 agcgctgccg ttagtaaaac ggaaaaagcg ctcgtccatg caattttctg attggggtag
     2101 aaatctgaga agcacaggta attaacagag gtcagcatta acaacaggaa tgcatccatg
     2161 ttgtgcaagt tgctaccccc gccgatcttg acgctcacga acagtccacc aatgaaaaag
     2221 acggataggg cggagatcaa aaatgtgcgc tggatccatc caaagtcttc cctcagccgg
     2281 ggcagagaga atcctaagaa taaaaggagc ggtgcagtta gccatagaat gccggttaaa
     2341 atccctttcg gaaacgttgg acttggaagc aggcggtacc atagcaggtc ggaggtgaaa
     2401 ctggtggtga agttttcaat agtactgttt cccgagagcg gaatatatgc catattggcg
     2461 atgaagctga agaaaatgcc aatgacgata tagaaaaaag gttctttgat atactcccac
     2521 gtcttcgtct tttctttttg cggagtttca aaaaagtaga gcagcgcagc cagcacaccg
     2581 gggaggggat accagtttat gcggctgatg cctgcccaac aagatgccag gatcacccac
     2641 aggcaggatt tccagaactt gtttcgatca aaaccaaata gaacaaggat agctgatatc
     2701 agcagatggt aatagatcgg accttgctgc aaaaaaagaa gccctgacaa aaccagaatg
     2761 gcattccacc agctatcttt ctgcttcaat cttttcacca tgccgatcat gcatatcagg
     2821 gtcgtggaaa gccagagaaa aacctgccag gcgcggtgga cgaaaatccc ggcgtttggc
     2881 agaagaaatg gcagagataa gatcacatac cgactggggt gcaggaatga aagggggagt
     2941 tgttgtccgt aaagcttctc ggagaataac aaggaggaat aataatagcg gctgccttca
     3001 gaccactcta atgtaaaggg gttgctggag atttcagcca ggtggttgcc cgcttgatag
     3061 gcgcatccaa gggctaagag actgaacaga agcaagccat tgaagggttt ttctcgataa
     3121 gctcccttta acatccctgc ggtgaggcag gcggcaaagt aaattgtgtg ggctcttaac
     3181 aatggtccac tgaaatagcg atcgaagcgg acgaaaatac aatagagaag cccacccata
     3241 aaaaccccaa aaagaaccca ggtcagcaag cgggaacgct tgaagggagc tgcaattttt
     3301 ttgaagagaa gtttgagccc cttgtctttt ttaaggatcg tgagaatgag cgttgtgaat
     3361 cccaggcagg caaggatgcc atttgcgtat ccgatgattt gccaggctga attcgtttca
     3421 taaaggccgt tgtttgtccc ttcaagaaag gcagcgagtg acagaagaca aaatagcaga
     3481 atgaaaagct tttgaagcaa atccgatttt tcaagaatct ggatgaactt tttcatgctg
     3541 gggtttcttc cctctgaaca ggttatgtac aaataattgt atctgctttt gaagcgcttt
     3601 aatttcggaa gttgtcagga gagaaaaaac aggggttaat aggagaaaga aacacaaacc
     3661 gatcaacccg atgagtattt ccagccaaag cttttctatc tgcaccagca ggctcaaaaa
     3721 aataattcct gatgacaccc cgacaattcg caagatcctc ttccaatcgt atgggatggg
     3781 gtggcttttt tgcgaattga aataaagagc aaagttgtag atcaagttcg ctgctagaac
     3841 aacataagca gttccccttt cagcatgccc cgggatgaaa aggagcaaac cacatcccat
     3901 gagcaaagcg cttatagcgg ttatccagcc gatatttttt gtgtttttgc tgataagcga
     3961 accgatcgaa acgatctgag aggtgccgga aagaacataa acaaatgcca gaggtgggat
     4021 gacctgcact gcggggagat attctctgcc agaaaagaag agcagaatgg gttttgcaaa
     4081 aagggaaact gccacggcga tcagcgaggc gaaatacaga taatacgaaa gagttttttg
     4141 ataaacggtg gatgcgtcag tttctttgga gacatcaaaa atgaaaggca gattcgctgt
     4201 ccggaacgcc tgaacgaaga aaccgaccac cgatgcaatg cgtagcccgg ctgaatagat
     4261 gccggttgct tccaatgtgg acatgctcga caagatcagg cggtcagccg aagccaaaaa
     4321 ccaggcgcta acgttcaccg ggatcaatgg tcgccagtag cgaaacattt ctttaaattg
     4381 agggaggtca aaatgcgcac tgaagaatga acggttgata tatattccaa tgacggtgaa
     4441 aaggatgtag caagctaaat tcgtccaaaa gatgctagcc atgcccccct gcattaataa
     4501 tacgaaaatg atgtttgcgg atatctgcag aagaataaac ggcagggtaa tggaaatgta
     4561 cacaatgggc ttgcgttgca ggcgcagcag attgagatta aaagtatata accccatcca
     4621 ggggattacc aggcaggcga tgcgcaataa atttgaaaaa tctgctgacc ccaacagcca
     4681 caggctgatc tgtttgctga aaactccaac caggagggcg accagggtat tgataagaaa
     4741 aacataaatg ctcagggttg agattgtctt tttccgctcc gaatcccctt tgtattcgta
     4801 gaaataaaaa ctctgtgcca gatcggttcc tgaagtcaat agaatgcttg ccagagcaag
     4861 aaaggtattg ataacctcta tggcgccata ttcggttaca ttgaataacc ttgaatataa
     4921 agggaaaagc agaaatgaaa tgaacttgct gccggcaccg cccaacccgt agatggtcgt
     4981 ttctttcaat actctttgag ttctggaagg catgggttgg ttaccggtta gtttttctca
     5041 tggaaacgtt tgtcttgctg catggttttt agaagtccct ccctcaaact gacgctgggg
     5101 cgatacccaa gtttcttttg agcaagtgaa atatctgcgc acatgcggga aggtcctgaa
     5161 tcatgatgtt ggttgtggac gatctctggt ttggttccgc tgagctctgt gaccaatctt
     5221 acaatctcat tcaccgatgt ctctctgcca cttccaacat tgatgatcag gcggttgacg
     5281 tcggatacag aagaagcgcg tatggctgcg ttgaccacat catccataaa cacataatcg
     5341 cgggtttgct tcccttctcc atgaataacg attgtgccat tatccagggc ttgttttatg
     5401 caataaggga tcactggcgc gtgcgcgggt ggtaagcgct gctctggtcc ataggcattg
     5461 aacacgcgta agatgaccgt ttcgattttc cataattcac cgattgtcct gatatagtat
     5521 tcggcggcta atttggaaac tgcatacgga gagcggggct gcggacgcag ggtttctttc
     5581 atgggttggt tgtgttgatt accgtaaata gagccggatg agataaatac gacacgtttt
     5641 acccctacat cgcgcatggc ttccatcagt gtaaccgttc caccgacatt taccaggtta
     5701 tattcacgcg gatagagaac cgattctggt acaatgaccc gggcagccag gtggtaaaca
     5761 caatcgacac cctgtaataa tgtccacaat tttggtcggt cgttaatatc ccctcttaca
     5821 aggtgcacgt cagatgaaag gcggtcgggg tctccggttg ataagtcatc cagtcctacc
     5881 acgctatgtc cgtcactgac cagactgttt gccagtggag cgccgagaaa acctgctgca
     5941 ccagtaatca gaaatctcat cggttatcca ttctaaaaga aatctagagc gcattatagc
     6001 atagatgggt agccgaactt tcataaagtg acaaagctga aagtgaagaa tgaagtaaaa
     6061 tggagaaata ttggattttg aaaatgacca tgaagaaaaa agcagtcttt actcttgtgt
     6121 tgttcaccct gctgttgggt gcgcatgttg gcgcagtctc tgctcagggc gagggaaccc
     6181 ccagttcaac cccattggca gttgagcctg tttcaaccga aacgcccaca gcgacggtga
     6241 atgcaggcgt ttcggacaag gatttgccgc tttgtttgcc cggcgcctat ttggaaacgc
     6301 ctgaagattg tctcccgctg ggttcatcgc tgatcttgac cgaactggca aggaaaggtt
     6361 tcacaattcc gctgaaaccg cttgccgcga cgaaaccgga caccagcttg gtagatctag
     6421 acatcaagta tgccaaattg aacctgcagg caggcatgca ggcggctttt taccccaacc
     6481 ttgactctgc cgtggctggc atcaatgctt tccgtttt
//
LOCUS       DAOSDS010000112         6494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4298_length_6494_cov_3.710825, whole genome shotgun sequence.
ACCESSION   DAOSDS010000112 DAOSDS010000000
VERSION     DAOSDS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4298_length_6494_cov_3.710825"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLL88_10120"
     CDS             complement(<1..661)
                     /locus_tag="PLL88_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HOJ01961.1"
                     /translation="MFFEIFIMLALIFLNGILSMAEMAIVSARKHRLQQKLENGKKGA
                     MDALDLKKNPNHFLSTVQIGITLIGILSGAFGGATIAEKIGSRLSNFKALAPYSEGIG
                     VAIVVILTTYFSLVIGELVPKRLALYDAEKISIAIAPIMKLLSKITKPIANFLSGSTN
                     VILGIFGIRPSSEPEITEEDLKVLLDEGTESGIFEKTEQDMVERIFRLNDRAISSFMT
                     PR"
     gene            complement(881..954)
                     /locus_tag="PLL88_10125"
     tRNA            complement(881..954)
                     /locus_tag="PLL88_10125"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(918..920),aa:Asp,seq:gtc)
     gene            complement(1016..1483)
                     /gene="ndk"
                     /locus_tag="PLL88_10130"
     CDS             complement(1016..1483)
                     /gene="ndk"
                     /locus_tag="PLL88_10130"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOJ01962.1"
                     /translation="MEENFEKTLVLIKPDGVQRKLIGPIISRFESRGLQLVAIKMVQV
                     SQELAETHYKEHWGKNFYSGLVNYITAAPVIAMIWAGEHAVEAVRQTVGKTNPLEAAP
                     GSIRHDFALLTSRNLIHASDSPATAEREIALWFKPEELCNWADQHHPWIFGKN"
     gene            complement(1496..3064)
                     /locus_tag="PLL88_10135"
     CDS             complement(1496..3064)
                     /locus_tag="PLL88_10135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ01963.1"
                     /translation="MSRIPLRKYVQEIEVLIENGNLDEAQKHCLYVLKTFPKSIAIYR
                     LLGKILMEKGEYEDAVYLFEKVLEVIPDDFISHVGLSFIAEVREQLDDAVMHMERAFE
                     LQPTNALLKEELKRLHLKKDGIEPQTIRLTRGALVNMYLRSTLYPQAIAELNIGLQTN
                     PNRIDFKERLAEALLYQGDKIAAVQTSIDILGKAPFSFSANRIAYEALPSTSEALDIQ
                     AFHRHLIEIDPYYAYVGDRIKAVEDVPDVAVSIERYNGKEPPIDLANLNVQEEIEKYW
                     NKPNSWKDQEALNAEIAWDSILDKKLANKQEETKAIAIDFVPETAGEAIEEPIEEKDN
                     LEQGMEINEQPSGNEIPGWIFDNPEEARHDDAPARPSTNSVISETPPAMELSQNESGM
                     VDQDVPTISEPESEIISKHWVKIENGQVNQSQSSLEDTRKIRLQESDDEIYASALRFI
                     EKNEFHTAIQSFNALLEKSAHVEDIIQTLERSTEILKQEPDSWYVLGKAYQKNNQKEK
                     ALHAFRKAEETSLS"
     gene            complement(3083..4315)
                     /locus_tag="PLL88_10140"
     CDS             complement(3083..4315)
                     /locus_tag="PLL88_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ01964.1"
                     /translation="MAKSKINVIEDEDDVIGYSAIARLIELGRQKSYVTYDDILHFFP
                     EAEQDLGQLEEAFATLMSAGIPYLEDDESLPEPTEDEILKDEEPESFDEEDLELDAEI
                     DDLRHIDTDDTIGLYLKEVSRVPLLTADEEVDLAQRIERGRMAREELAKSTVTDGRRA
                     ELRELIEDGWAAREHLVTANSRLVISVAKKYMGRGVPFLDLIQEGNIGLIRATKKFDY
                     RRGHKFSTYATWWIRQAVTRAIADQGRTIRVPVHMGDQINKLLRIQHQLTQKLGRDPS
                     IDELADALDVTPKKVENMIKVSRRPLSLETPTDNEDDSVLGDFIEDDEVPAPDETATY
                     NLLKEHLSVILNSLPPREVRILQLRYGLLDGQAYTLEEVGRKMGVTRERVRQIEAQAL
                     SRLRHPTVRRKLRDYLGE"
     gene            complement(4512..5204)
                     /locus_tag="PLL88_10145"
     CDS             complement(4512..5204)
                     /locus_tag="PLL88_10145"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ01965.1"
                     /translation="MTHIHSLTPLYMDDEILVINKPAGLPTIPDGYDLSKPCLRNLLS
                     QQYGRVWVIHRLDKETSGVILFARTANAHRKLNSQFESHRIRKIYLALIYGFPCWEQR
                     TVDYPLHKDGDRKHRTVVDEKNGKLALTIISVLKRADNHSLIAVEIKTGITHQIRAHL
                     SLLGFPIIGDSLYAFYALRSTNRYLPFEQGRMYLHAFCLDFIHPATNDHTYIEAPIPA
                     YFQDALNPDNAQ"
     gene            5260..5571
                     /locus_tag="PLL88_10150"
     CDS             5260..5571
                     /locus_tag="PLL88_10150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOJ01966.1"
                     /translation="MLIVIVHVHVKEEYISAFCEASIENATNSSQEEGIFSFDVIQEN
                     DDPSKFALVEVYKDAAAAAAHKETDHYKKWKDTVAAMMAEARFGVKYHEIYPPEKSWR
                     K"
     gene            complement(5733..>6494)
                     /locus_tag="PLL88_10155"
     CDS             complement(5733..>6494)
                     /locus_tag="PLL88_10155"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOJ01967.1"
                     /translation="GVMQYLEEQKIGVETGYARVPIVPSAILYDLGIGSSTVRPNAEM
                     GYQACCDATSNAPQNGNFGAGTGATVGKILGMNQAMKSGIGSAAMEISHGIYVGAIIA
                     VNAFGDVIDPSSNTIIAGTRSLSKGPIKIGEPGYFANTQEVMRSMVGKTVMKIAAKQN
                     TVIGAVVTNADLTKEEANKVAQMGQDGLARAIRPAHTMLDGDTLFTIATGKKKCDVNT
                     VGAFAAEMVAEAILSAVRNAEAVGSLPSHQSISRE"
BASE COUNT         1608 a         1514 c         1500 g         1872 t
ORIGIN      
        1 ttcttggcgt catgaaagaa ctgattgcac ggtcattcaa acgaaaaatg cgttcaacca
       61 tatcctgctc agttttttcg aaaattccac tttctgttcc ctcatctaac agtaccttta
      121 agtcttcctc ggtaatctca ggttcagaag acggacgaat accaaaaatg ccgagaatga
      181 cattcgtaga accactgaga aaatttgcga taggttttgt aatttttgaa agcaacttca
      241 taatcggtgc aatcgcaatg gaaatttttt cggcatcata taaagccaag cgtttaggga
      301 caagctcccc aattacgaga gaaaaatatg tcgtaaggat aacgactatg gcaacaccaa
      361 ttccttcact atagggtgcg agtgctttaa aatttgatag ccgtgaaccg attttttctg
      421 cgatcgttgc gccaccaaat gcgccactta atatgccaat cagtgttatt ccaatttgaa
      481 ccgtcgataa aaaatgattg ggattctttt ttaaatcaag tgcatccatt gcaccctttt
      541 tcccattttc taatttctgt tgaagacgat gttttcttgc tgaaacaatt gccatttctg
      601 ccattgacag aataccgttt agaaaaatta gagcaagcat tataaaaatc tcgaaaaaca
      661 ttgcgcctca caatccggtt tcatatttaa attaataaag agacactaga attgcttaac
      721 caacagaatt ctagattgtg aacgagaaaa aagtgttttt gatttgctaa tgggtggtgg
      781 tcaggatggt ctgaatcgtc ttcttctgat gattcttctg cagacttttc agttttaatt
      841 gaaatcatta gtatttcttt cgtatttctc gaaaaaagag cgggagcgac gggattcgaa
      901 cccgcgatct cggccttgac agggccgcat gttaaaccgc tacaccacgc ccccgtgatg
      961 tgagcaagag tttaacacag gaagcacaca gcgtcaagcg aattcttgaa ccgatctaat
     1021 tttttccaaa aatccagggg tgatgctgat cagcccaatt gcatagttct tctggcttga
     1081 accacaaggc gatttcgcgt tcggcagtag ccggtgagtc ggaagcgtgg atgaggttgc
     1141 ggctggtgag cagagcaaag tcatggcgga tgctgccagg ggcagcttct aaagggttgg
     1201 tcttgccgac ggtctggcgg accgcttcga cagcatgttc acccgcccat atcatggcga
     1261 ttacaggagc cgcggttata tagttaacca gcccactgta gaagtttttg ccccaatgtt
     1321 ctttgtaatg tgtttctgcc agttcctggc tgacctggac cattttaata gccaccaatt
     1381 gaagaccgcg actttcaaac cgagaaatga tcggaccgat caatttccgt tggacaccat
     1441 cgggtttaat cagaacgagg gttttttcaa agttttcttc catgcgtatc ttcctctacg
     1501 aaagtgatgt ttcttcggct tttcgaaatg cgtgcagcgc tttttctttt tggttgtttt
     1561 tttggtatgc tttgcccagc acataccagc tatcaggttc ctgtttgaga atttcggttg
     1621 aacgctccaa tgtttgaatg atgtcttcta catgggctga cttttcgaga agggcattga
     1681 aactttgaat ggctgtgtga aattcgtttt tttcgatgaa gcgaagtgct gacgcataaa
     1741 tttcgtcgtc gctttcttgc agcctgatct tcctggtatc ttccagactg gattgggatt
     1801 gattgacctg tccgttttcg atctttaccc aatgttttga aatgatttca gattccggtt
     1861 cagaaatggt tgggacatcc tgatcgacca tcccgctctc gttttgactc aactccatgg
     1921 caggtggagt ttcgctgatc accgaattgg tggaagggcg ggcaggggcg tcatcatgcc
     1981 tcgcttcttc aggattatca aaaatccatc cgggaatctc attaccagaa ggctgctcgt
     2041 tgatctccat gccttgctcg agattgtctt tttcttcaat cggttcttca atcgcttccc
     2101 cggctgtttc gggcacaaaa tcgattgcta tcgctttcgt ttcctcttgt ttattggcaa
     2161 gcttcttatc aagtatgcta tcccacgcta tttcagcatt caaggcttct tgatctttcc
     2221 agctattggg cttgttccaa tacttttcga tttcttcttg aacgttcaaa ttggcaaggt
     2281 caattggggg ttcttttccg ttgtaccgct caatgctgac agccacatcc ggaacatctt
     2341 ctaccgcctt gattcgatca cctacgtaag cgtaataggg gtcgatctcg atgagatggc
     2401 gatggaaagc ctgtatatcc agagcttcgg atgtggaggg aagagcctcg taggcgatac
     2461 ggttggcgct gaaactaaat ggagcttttc ccaggatatc gatgctggtt tgcaccgctg
     2521 caatcttatc gccctggtac aagagcgcct cggcgaggcg ttctttgaaa tcgatacggt
     2581 tgggattggt ctgcaaaccg atattcaatt cggcaatcgc ttgcgggtac aatgtgctgc
     2641 gcaaatacat attgaccaat gctccgcggg tcaggcggat agtttgcggt tcgatgccat
     2701 ctttttttag atgaagacgt ttgagttctt ctttgagaag cgcattggtt ggctgcaact
     2761 caaaagcacg ttccatgtgc ataacagcgt cgtcgagctg ttcacgcact tcggcgataa
     2821 agctcaaacc cacgtgggaa atgaagtcgt caggtatgac ttcgagaact ttttcgaaca
     2881 gatataccgc atcctcatat tcgccttttt ccatgaggat ctttcccaac aatcggtaaa
     2941 tagcaatgct cttagggaat gtttttagta catacagaca gtgtttttgg gcttcatcaa
     3001 ggtttccgtt ctcgatcaag acttcaattt cttgaacgta ttttcgtaaa ggaatccttg
     3061 acatagccac ctagtcccaa ttttattctc ccaaatagtc gcgcaatttc cgtcgaacgg
     3121 tcgggtggcg cagccggctg agagcctgcg cctctatctg gcggacacgc tcgcgcgtca
     3181 cgcccatctt tctgccgacc tcctccagcg tataggcttg cccatccaat aacccatagc
     3241 gcaattgcag tatgcgcact tcacgcggtg ggagtgaatt taatattaca ctaagatgct
     3301 cttttaataa attatacgtc gcggtttcat ccggtgcggg aacctcatca tcttcgatga
     3361 aatcgcccag tacagaatca tcttcattat cagtcggtgt ctccaaagaa agcgggcggc
     3421 gggatacttt gatcatattc tccactttct ttggcgtcac atcgagtgca tcagccagtt
     3481 cgtcaatact cggatcgcga cctaatttct gcgttaattg gtgttgaatg cgcagtagct
     3541 tgttgatttg gtcgcccatg tgtaccggga cgcgaatggt gcgcccctga tcagcaatgg
     3601 cacgggtgac cgcctggcgg atccaccacg tggcataggt gctgaattta tgcccacggc
     3661 gataatcgaa tttcttggta gctcgtatca atccgatgtt tccttcctga atgagatcga
     3721 ggaaaggaac accgcgtccc atatactttt tggctacact gatgaccaaa cgcgaattgg
     3781 cggttaccag gtgttcacga gctgcccatc catcttcgat caactcacgc agttctgctc
     3841 tgcgtccatc ggtgacagtg ctcttagcca gttcttcgcg tgccatccga cctcgctcga
     3901 tgcgttgagc caaatccact tcttcatcag ccgttagcag cggaacgcgg cttacttctt
     3961 ttagataaag cccaatggtg tcgtcggtat cgatatggcg aaggtcatcg atttccgcgt
     4021 ccagttccaa atcttcttca tcgaaacttt ccggttcctc gtccttgagg atctcatctt
     4081 ctgtgggttc gggaaggctt tcgtcatcct ccagataggg aatgcccgcg ctcatcaacg
     4141 tggcgaaggc ttcctctaat tgccctagat cctgttcagc ttcggggaaa aaatgcaata
     4201 tatcatcata ggttacatac gatttctgtc gaccgagttc gataagcctt gcgattgcgg
     4261 agtaaccgat gacatcgtct tcatcttcga taacgtttat tttgcttttt gccataaatt
     4321 aaatacgacc ttttccataa aaataaacaa aattatggca cgcaaaaaac aattactctt
     4381 tatgagcata cctgttttca aaataaaaag caataatact tttgttttat agaaaaatct
     4441 tacaagtttg cgtgctgcgc aagattctac tatacacaat tgacaacgaa aagtcaacaa
     4501 ccaattgtgc gttattgtgc gttatcagga ttcaaagcgt cttgaaagta ggcgggaatt
     4561 ggtgcttcga tatacgtgtg atcgttggtg gctgggtgga tgaaatcaag acagaaagcg
     4621 tgcaggtaca tacggccttg ctcgaaaggg aggtacctgt ttgtggatcg taaagcataa
     4681 aatgcgtaga gtgaatcgcc aatgatcgga aaacccagca aagaaaggtg tgccctgatc
     4741 tggtgggtga tgcctgtttt gatctcaact gcgatcaggg agtgattgtc tgcccttttc
     4801 aatacagaga tgatcgttag cgccagcttg ccatttttct cgtcaactac tgtgcggtgc
     4861 ttccggtcac catccttgtg gagagggtaa tcgaccgttc tctgttccca acaaggaaag
     4921 ccataaatca aagccagata gattttgcga atacggtggc tttcgaattg ggagttcaat
     4981 ttccggtggg cattcgcagt tctggcaaag aggatgaccc cactggtttc tttgtccaga
     5041 cgatggatca cccacaccct tccatattgc tgcgaaagga gattcctaag acagggcttg
     5101 gaaaggtcgt agccatcagg aatggtcggc agcccggctg gtttgttgat aaccaggatc
     5161 tcatcgtcca tatagagagg agtcaatgaa tggatatgcg tcatgctcga gaactctgct
     5221 ttataataat ctcaccttag aaaagatcgg ggatcggtca tgttgattgt aattgtacac
     5281 gttcatgtaa aagaagaata catcagcgcg ttctgtgaag ccagcattga aaatgcaacc
     5341 aacagttcgc aagaagaagg gattttttcg tttgatgtga ttcaagaaaa tgatgatcct
     5401 tcaaaatttg ccctcgttga ggtgtacaaa gatgctgctg ctgcggcagc tcataaggaa
     5461 acggaccatt ataagaaatg gaaagatacc gtagcggcaa tgatggctga agcgcgtttt
     5521 ggtgtgaaat atcatgaaat ctaccctccc gaaaaatcct ggagaaagta acaattgccg
     5581 tgcgtttcag tcgcagcaat tttcgtcgtt ccaacgaggc gaattaagca agacggctaa
     5641 ttgagaagaa accctgcttt tctctctacc tatggggata cggttggaaa aatcctggca
     5701 ctcaggaggt ggagggttcc agccctttga tattattctc tggaaatcga ttggtgggat
     5761 ggaaggctac ccactgcttc ggcattcctc actgcgctga gaattgcctc cgctaccatc
     5821 tccgcggcga aggcgccaac agtattcaca tcacatttct ttttccctgt ggcaatcgtg
     5881 aaaagcgtat ctccatccag catggtgtga gccggtcgga tggcgcgagc caatccatcc
     5941 tgtcccattt gcgctacttt gttggcttct tcttttgtga ggtcagcgtt cgtcactact
     6001 gcaccgatga ccgtgttttg cttggcagcg attttcatga ctgtctttcc aaccatggaa
     6061 cgcatgactt cctgcgtatt ggcaaaatag ccaggttcgc cgatcttgat aggacccttt
     6121 gatagagaac gggtacccgc tatgattgtg ttcgaagatg gatcgatcac atccccaaat
     6181 gcattcacgg cgatgatggc tcctacatag ataccgtgac tgatctccat agctgctgac
     6241 ccgatccctg atttcatcgc ctggttcatc cccaagattt tgccaactgt agcacctgtg
     6301 ccggcgccaa aatttccgtt ctgaggggcg ttggatgttg cgtcgcaaca tgcctgatat
     6361 cccatttcag cgtttggcct gacggtagaa gaaccgatcc ccagatcata aagaatggca
     6421 gaaggcacaa tgggcaccct ggcataaccg gtttccacac ctatcttctg ctcctccaaa
     6481 tattgcatta cgcc
//
LOCUS       DAOSDS010000113         6460 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4332_length_6460_cov_4.283060, whole genome shotgun sequence.
ACCESSION   DAOSDS010000113 DAOSDS010000000
VERSION     DAOSDS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4332_length_6460_cov_4.283060"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="PLL88_10160"
     CDS             complement(<1..920)
                     /locus_tag="PLL88_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOJ01968.1"
                     /translation="MGHEMPEKNYSRIPKVLDIPNLIQVQKESFDRLLSEGLIDLFDE
                     ISPIESYNGGVKLYFPGKSDISKQWGLKYWFEDPKNSIEECVEKDLTYSSPLYVKVLL
                     AGSEIAEPMVEDIYLGEFPIMTENGTFIINGTERVVVSQLIRSPGVYFDAPINRATGK
                     RLATAKLIPDRGAWMEFETRKNDYIILKFNRKRTVPITIFLRALAAVDDGLKDSPLKY
                     GTDEELISLFNDIDTDQDRLFIPTTFHQEPEWDLSGSKTIAELALLEFFRRMRPGDPA
                     TIENAKEFLEEQLFDQRHYDLENVGRYKLN"
     gene            1104..4793
                     /locus_tag="PLL88_10165"
     CDS             1104..4793
                     /locus_tag="PLL88_10165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ01969.1"
                     /translation="MNNKTQKHEISLKAFFGKFSWYSALIILIIIAAILTRFMNLGER
                     VMSHDEVNHVVPSYDLFAGLGYQHNPMTHGPLQFHLITFSYFLFGDSDFSSRVPHALA
                     SVATIIFLLFAYHRYFGKSGAAITSILFLISPFVMFYGRYARNEVFVGLFLVLIIYAF
                     MRHYEKRDHHSLFLIAIALSLYFTSKETSYIHTAIFMIFFFIKMLPEISLSKPAHLRA
                     FLGSLLLILVIAISLVGSIYLFRQTDLSNIDNNALILQSSTDLPFKTQLYYSASSFKS
                     LLPGIIPLFIGILTVLYGKKFLRWDLFIPSPAFNIFMLITTLTLPLLAAFPVKFCGYD
                     PTAYSSATSNLLDFVYLVSLAAIAIILGFAWNRDFWWKAAGIFYFIFFILYTTFLTNM
                     QGALTGTIGSLGYWLAQHDVNRGNQPLYYYALLQMPFYEFLGLAGSALAVILAAFKRR
                     MKTSEDSKATTTAEGSAVEEGEPEPTKPVLSTTVFFLFLGISSLIAFTIAGEKMPWLT
                     MQITIPFLLLTGKTLGDYLDSIAWKDRSIRERVIGVLISFLIIADFCSIGVTLLGNNL
                     PFQGKSQAQLQVTYIFLFRLVLLAGLLLLYRTSLHGWSKNVRTKTFVVALFLLLGCIT
                     GQSALRASFENYDYATEYLVYAHGAPGPKEILAQVEKISQTLTGGLDIRVAYDNDSLY
                     PFWWYFRHYPNRIAYMENVTRDLETVPIILAGQANYDKVEPLIRKDYYSFEYFRLWWP
                     NQDYFNLTFDRIVEVLKSPDLRQAVFNIWYYRDYSLYATLNNNLYLDITTWQPSQKMR
                     MYIRKDIAEQVWDLISDDALIQSPTGDAFAAITTTISPTRFIGGDDVLNEPRGIDIAP
                     DGSIYVADSKNNQIKHFSTTGVLLDSWGSFANAADTTTSAVGGTFYEPWDVAVAPDGT
                     VYVADTWNHRIQKFTSDGQFISMWGYFATDNTPLAYYGPRGITVDADGNVYFTDTGNK
                     RILIFDKDDAFVAQIGSAGSDYGQLNEPVGIALDENNHLYVADTWNSRVQVIVPDLEL
                     NTSTPITSWNVEGWYGQSVNNKPFIAVNAQGHVFVTDPEGALILEYSQDGVLVHAWDI
                     RGISDDTISMPVDIEFAEDGTMWVSDAASNMIYGYDISEDGSTEEVAQTQVSPTPDLL
                     LITEAQLSLLHTKTHQDMINAAYQAADEVGISPWVLLRAAGWGNFTDDRGALYAGEHI
                     MDIKTLDEIQKEMLLKYLGIPNSLE"
     gene            complement(4805..5746)
                     /locus_tag="PLL88_10170"
     CDS             complement(4805..5746)
                     /locus_tag="PLL88_10170"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ01970.1"
                     /translation="MMNKSIRPLHVATDLYQIADLLSICFSDSMDEDGERYIAYLRQF
                     SQSLLFTKIAERYPEKYSLPGEGFVYEDGGRVVGNITLSPMKFEDETVYLISNVAVEP
                     EHRGRGIAKLLTQTALRYVECKGVKQVWLQVKQGNQAAIKLYDQLDFQSFMVRTTWMR
                     KKTNVFLKRPEKFALRKRKKGEWKIQRELLESTYPVELTAAYEFEIASVQPNLKRTIR
                     DFFLSRVTLHWAAEINEQLGFVSYASLQDQSYVNLWIAAPVGYEQQFLKTLVPAVQAR
                     LGKEVRLNYPQHEYGDCFHELGMQELNTLVWKRKYLV"
     gene            5842..>6460
                     /locus_tag="PLL88_10175"
     CDS             5842..>6460
                     /locus_tag="PLL88_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HOJ01971.1"
                     /translation="MMRFDRFTERAQEAAQKAAEIIQRYGHNQIDTEHILLALIEQQQ
                     GVVGQLLEIMSVDINGLRDRLDQMLRESPKASIYGGGTGQVFITPRVKRVIEVANQEA
                     SNLNDEYISTEHLFLAIVEEKDTPSSRLLKENGITKERVLDAIQELRGGQKVTDRKAE
                     TRYRTLEKYSRDLTKLAAENKLDPVIGRDNEILRVIQVLSRRSKNN"
BASE COUNT         1686 a         1563 c         1406 g         1805 t
ORIGIN      
        1 tggttcagtt tgtagcgacc gacattctcg agatcatagt gccgctggtc aaaaagctgc
       61 tcttccaaaa attcttttgc attttcaatg gtggcaggat cgccagggcg catacgccgg
      121 aagaattcta acaacgccaa ttcagcgatc gtcttgctgc cagacaggtc ccattcgggt
      181 tcttggtgaa acgttgtggg aataaacaac cgatcttggt cggtgtcaat atcattgaac
      241 agagaaatca attcctcgtc agtcccatac ttcagagggg agtcttttaa tccatcatcc
      301 actgctgcca acgctcgtaa gaaaatggta atgggaaccg tgcgtttgcg attgaacttc
      361 agaataatgt aatcattttt acgcgtttca aattccatcc aggcaccgcg atcggggatt
      421 aattttgcgg tggctaatct ctttccggta gcgcggttaa tgggtgcatc aaaatacaca
      481 ccgggcgaac ggatcagttg agatacaaca accctttcgg ttccattgat gatgaaagtt
      541 ccattttctg tcatgatggg gaattcaccc aaatagatat cttccaccat gggttcggcg
      601 atctcagaac cagccaacag gaccttcaca tacaatgggc tcgaataagt caggtctttc
      661 tcaacacatt cttcgatcga attcttagga tcttcaaacc aatacttcaa cccccactgc
      721 ttactgatat ctgattttcc gggaaagtaa agcttcactc ccccgttata cgattcgatc
      781 ggcgagatct catcaaacag gtcgatgaga ccctcactta ataatctgtc gaaagactct
      841 ttttgaacct ggattaaatt gggtatatca agaactttcg ggattcgcga ataattcttt
      901 tcaggcattt catgacccat gtatctctcc tgattattga acaatttaaa tacattacca
      961 acgccatacc tatggcaaac gaatatttta tcaacaatag acacattggt caagcaagtt
     1021 tattctcgaa ttgcaatgaa tttttcatta tagcgcaaga attacaatta ttggttagaa
     1081 ttgatgggaa tcaaaaggac ccaatgaaca ataaaacaca aaaacatgaa atttcgttaa
     1141 aggctttctt cggcaaattt tcatggtatt ccgctctcat cattcttatc attatcgcag
     1201 ccatcctcac ccgttttatg aatctgggcg agcgggtgat gagccacgat gaagtgaacc
     1261 atgttgttcc atcctacgat ttgttcgccg gtctggggta tcaacataat cccatgacgc
     1321 acggaccact tcagttccat ttaattactt tttcctattt tcttttcggc gattctgact
     1381 tcagttctcg tgttccccat gcgctggcaa gcgtagcaac catcattttc cttttatttg
     1441 cataccatag atacttcgga aagagcggtg cggctattac gtcaatcctg tttcttatct
     1501 ctccgtttgt gatgttctac ggtcgatacg cgcgcaatga agtttttgtt ggcttgttcc
     1561 tggtactcat catctacgcc ttcatgcggc attatgaaaa aagagatcac cattccctct
     1621 ttctgattgc tatcgccctc tccctttatt tcacctcgaa agagacatcg tatattcaca
     1681 ctgccatctt catgatcttt ttctttataa aaatgctgcc cgagatctcc ctttccaagc
     1741 cagcccacct gcgggcattt ctgggcagtc tcttgctcat ccttgttatt gccatatcgc
     1801 tagtgggctc catctacctg ttccgccaga ccgatctgag caatattgac aacaatgcgc
     1861 tgatcctgca aagcagcaca gacctcccct tcaaaacgca gttgtactac agcgcatcgt
     1921 ccttcaaatc gctattaccc ggaatcatac cgctgtttat tggcatcctg accgtcttat
     1981 acggaaaaaa gtttctgcgt tgggatcttt ttattccatc accggcgttc aatatcttca
     2041 tgctaatcac aacacttacg cttcccctat tggcagcatt cccggtgaag ttttgtggat
     2101 atgatccaac cgcttacagc agcgcaacca gcaatctgct ggatttcgtc tatcttgttt
     2161 ccctggcggc gatcgccatt atcttaggat ttgcatggaa tcgtgatttt tggtggaaag
     2221 cagccggcat cttttatttt atttttttca tcctctatac aacttttctg accaatatgc
     2281 agggtgcttt gaccggcacc attggttccc tgggttattg gcttgcccag cacgatgtaa
     2341 accgcggcaa ccaaccactc tattattacg ccctgctgca aatgcctttc tatgagtttc
     2401 tggggttggc tggttccgct ctggctgtta ttctggcagc ctttaaaagg agaatgaaaa
     2461 ccagcgaaga cagcaaagca acaactacag cagaagggag cgccgtcgaa gagggagagc
     2521 cagaaccaac aaagcccgtt ctttcaacca ccgtcttctt tctgttcctg ggcatttcca
     2581 gcttgatcgc ctttacgata gccggcgaaa aaatgccctg gctgaccatg cagatcacca
     2641 ttccattttt gctgttaaca ggaaaaacgt tgggagatta tcttgattcc atcgcatgga
     2701 aagatagatc gattcgtgaa agagtgatag gtgttctgat cagctttttg atcatagccg
     2761 atttttgttc catcggcgtc accttgctgg gcaacaattt gccctttcaa gggaaaagcc
     2821 aggcacagct tcaagttacc tatatttttc tctttcggct ggtattgctg gctggattgc
     2881 ttctcctcta ccgcacctcc cttcatggct ggtcaaaaaa tgttcgaaca aaaacatttg
     2941 tggtggcgct tttcctcctt ctcggttgca taaccggtca atccgctctc cgtgcctcat
     3001 ttgaaaatta tgattatgcc accgaatacc tggtctatgc acacggcgcg ccgggtccca
     3061 aagaaatcct cgctcaggtc gagaagatat cacagacatt gaccggcggc ttggatattc
     3121 gggtagctta cgataacgat tctctctatc ccttttggtg gtatttcagg cactacccca
     3181 atcgtattgc ttatatggaa aatgtgaccc gtgatcttga gactgttccc atcatcctgg
     3241 caggtcaggc aaattatgac aaggtcgaac cgcttatccg taaagattat tacagctttg
     3301 aatatttccg tctgtggtgg cccaatcaag attacttcaa cctcactttc gatcgaatcg
     3361 ttgaggtcct gaaatcccct gacctgcggc aggctgtttt caacatctgg tactatcgcg
     3421 attattcgct ttacgccaca ttgaataaca acctttacct ggatataacg acctggcagc
     3481 ccagccaaaa aatgcgtatg tatattcgca aagatatcgc agaacaggtg tgggatctta
     3541 tttctgatga tgcgctaatt caaagcccaa ccggcgatgc gtttgcagca atcaccacga
     3601 ccatttcccc aacgcggttt attggcggag atgacgtgct caatgaaccc cgtggaattg
     3661 atatcgctcc ggatggttct atttatgtag ctgattcaaa aaataaccag atcaaacatt
     3721 tctcgaccac tggagtactt ctggattcct ggggcagttt cgccaatgcc gccgatacca
     3781 ccaccagcgc tgtgggtggc actttctatg aaccctggga tgtggcagtc gcgccggatg
     3841 gaaccgtcta tgtagcggat acctggaacc accgtattca aaaattcacc agcgatggac
     3901 aattcatctc catgtgggga tatttcgcca ccgataatac accgctggcg tattacggac
     3961 cgcgcggcat cacggttgat gccgatggca acgtttattt tacagatacc ggcaacaagc
     4021 gcattttgat ttttgataag gatgacgcct ttgtggctca aattggaagc gctggttccg
     4081 attatggtca attgaatgag ccggttggca tcgccctgga tgaaaacaac cacctgtatg
     4141 tagcggatac ctggaattca cgagttcagg tgatcgtacc tgatcttgag ttgaacacct
     4201 caacccccat cacaagctgg aatgtggaag gttggtatgg tcaatcggtc aacaataaac
     4261 cattcattgc agtaaacgca cagggtcatg tgttcgtgac cgatccggaa ggggctttga
     4321 tccttgagta ttcccaggat ggtgtgctgg tgcacgcctg ggacatccgg ggtatttcgg
     4381 atgatacgat cagtatgcct gtggatattg aattcgcaga agatggaacc atgtgggtca
     4441 gtgatgccgc cagcaacatg atctatgggt atgacatttc tgaagacgga tcaacagaag
     4501 aagtggcgca aacgcaagtg tcgcccacgc ctgacctttt gttaatcacg gaagctcaat
     4561 tgagcctgtt gcatacaaaa acacatcaag acatgatcaa cgcagcctat caagcagccg
     4621 atgaagtggg catctctccc tgggttttac tccgagctgc aggctggggg aatttcaccg
     4681 atgatcgcgg cgcattgtac gcaggggagc acatcatgga cattaaaacc ctcgatgaga
     4741 tccaaaagga aatgctcttg aagtatcttg gcataccaaa ttcattggaa tgaagtgttt
     4801 ttttctaaac aagatatttt cgtttccaga ccagtgtatt taattcttgc attccaagct
     4861 catggaagca atcgccatat tcatgctgcg ggtaattcaa gcgcacctct tttccgaggc
     4921 gcgcctgcac agcggggaca agggttttca aaaattgttg ttcgtatcct accggagccg
     4981 caatccatag attgacatac gattggtcct ggaggctagc ataactgacg aacccaagtt
     5041 gttcattaat ctcagcagcc caatggagag tgacacgact gaggaaaaaa tcgcgaattg
     5101 tccttttcag gttgggttga acggatgcaa tttcgaattc gtaggcggct gtcaattcaa
     5161 caggataggt gctctctaac aactctctct ggatcttcca ttcccctttt ttccttttcc
     5221 gaagagcgaa cttttccgga cgttttagaa acacgttcgt ttttttccgc atccaggtgg
     5281 ttcggaccat aaacgattga aagtccaatt gatcatatag cttaattgcc gcctggtttc
     5341 cctgtttcac ttgcagccat acctgtttca ctcctttgca ctcaacatag cgcaaagctg
     5401 tttgggtcag aagtttggca attcctctcc cgcgatgttc cggttctaca gccacattgg
     5461 aaatcaagta gaccgtctca tcctcgaact tcatgggaga taacgtgatg tttcccacca
     5521 cccttccccc atcttcatag acaaaaccct cgccaggcaa agaatatttt tctggatagc
     5581 gttctgcaat tttcgtaaaa agcaagcttt gcgaaaattg gcgcaaatat gcaatatagc
     5641 gttcaccatc ttcatccatg gaatcagaaa aacagataga aagcagatca gcgatctggt
     5701 aaagatcagt tgcaacgtgc agagggcgta tggatttgtt catcatgaat ggttctttaa
     5761 ttcttatatt ctttttatgg ctttggtata tgatactgat agcaaatgtt ataatgccga
     5821 ttgacatagg agacaagtac tatgatgcga tttgatcgat ttaccgagcg tgctcaagaa
     5881 gccgcccaaa aagcggcaga gatcatacaa cgctacggtc ataatcaaat agatacagaa
     5941 catatcctgt tggctttgat cgagcaacag caaggcgtgg ttggacaact tctggagatc
     6001 atgtcggtgg atatcaacgg tttacgcgac cgattggatc agatgttgcg agaaagcccg
     6061 aaagccagca tttatggcgg cggtacaggg caagttttta taacgccacg tgtaaaacgc
     6121 gtgatcgagg ttgcaaacca agaagcgtca aacctgaatg atgagtatat ttccacagaa
     6181 catctttttt tggcaattgt tgaagaaaag gacactccgt cctcccgcct gttaaaagaa
     6241 aacgggatca ccaaagaacg tgtgttggac gcaattcaag aactgcgtgg ggggcagaaa
     6301 gtgacagacc gtaaagcaga gacccgctat cgcactcttg aaaaatattc acgcgacctc
     6361 accaaactgg ctgcggaaaa caagcttgac ccggtcatcg gtcgcgataa cgagatcttg
     6421 cgcgtgatcc aggtgctctc ccgccgctca aagaacaatc
//
LOCUS       DAOSDS010000114         6304 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4460_length_6304_cov_3.513682, whole genome shotgun sequence.
ACCESSION   DAOSDS010000114 DAOSDS010000000
VERSION     DAOSDS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4460_length_6304_cov_3.513682"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1812
                     /locus_tag="PLL88_10180"
     CDS             <1..1812
                     /locus_tag="PLL88_10180"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ01972.1"
                     /translation="RAAAYPDRYGGIHGMIQDKNMENKINSNSPKAGRVIGTVIGGSL
                     SESLSARLQVSPQEIQEGSFVTIKSGDWQFYGLITDLRLGATDPRFSDEQIDAYYPAA
                     IARALREEVLYSNAVILPVLMLENGPEMNADNAQQVMGWQQQKEENGEMMTRPIQIKT
                     IPAHQSAVRLANAVDIATVFGDPSEEDNFVIGYTREQNFPVCIDLAHFIQRSAGVFGA
                     TGTGKSFLTRILLAGLIQQAKACLLVFDMHDEYGFDDTASDSGEKVTGLHTHFGPKVQ
                     VVGLGGGKNIRGNSPDFHVEIAESDIEASDIEMLSVELNLKETTATTLAALSASFGKR
                     WFSEFKDMKAGSTIEDENGKKVPAPDSVVWWANQNGVNVMAAESLHSKLGRLFHAGYI
                     TAKPAGNGLQSVVDLLKNGKHVVLSFSGYDNDLDYLFISNLITRKIREEWEQLTNAYR
                     NGRSDASEPCQLVIVVEEAHKLLNREMASQTTFSTIAREMRKYYVTLLIVDQRPSQIY
                     DEVLSQLGTRICGYLTDDEDIRAVLSGAAGKDALRSMLAHLQPKEEVLIAGWGVPMPI
                     PVRSRRLDAGFWEELKKGRASTLHSGDMETQKKMLGF"
     gene            complement(1809..2270)
                     /locus_tag="PLL88_10185"
     CDS             complement(1809..2270)
                     /locus_tag="PLL88_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HOJ01973.1"
                     /translation="MNIFQQLLSNYVLMSAATAWLVAQLLKLPINYFFNKEWDWSILI
                     SPGGMPSSHSAISTAAALGIGLQEGFGSSLFALAMTLATIVIYDAISIRRQAGFHAEW
                     INKIVEEIFKSSSFSYQKLQEVLGHTPFEAIGGVVLGIFNALIMWVIYYGL"
     gene            complement(2267..2503)
                     /locus_tag="PLL88_10190"
     CDS             complement(2267..2503)
                     /locus_tag="PLL88_10190"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOJ01974.1"
                     /translation="MNTKIDISKLKYEEALQQLEQVINHLESGVLELDEMLARFEQGK
                     DLLAHCQQLLDQAELRVREIGQLEEKSSDQEDQP"
     gene            complement(2505..3740)
                     /gene="xseA"
                     /locus_tag="PLL88_10195"
     CDS             complement(2505..3740)
                     /gene="xseA"
                     /locus_tag="PLL88_10195"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOJ01975.1"
                     /translation="MLTGSLFSRQIFSVSEVTGYIRVLIEENEDLQDIWVQGEISNLS
                     SPASGHIYFTLKDEAASLRCVIWKWMAQSLHFRPQDGMAVDVHGKFGVFEKGGQYQFY
                     VDSMRQQGEGRLFQEFLELKNKLEAEGLFAEELKKNVPAFPTVIGVVTSSSGAALQDI
                     LNTLRSRYPLAQVFLSATLVQGEGAPHNIVQALHLLYNASTRPDVIILARGGGSLEDL
                     WSFNDERVVRAVAASPIPIISGVGHETDFTLVDFAADLRAPTPTGAVVLAVPDIQDLK
                     LDLQDRKSLLVSLGSQLVQMKTAEMKELYYRLLQNSPERLLQQNMLQLDEVGRRLQYT
                     LYSRLAEFKAHFGEKGARLLALDPQGVLRRGYALLYDGQGKLIDSVTKTGENEMVHVR
                     LKDGSLQARITAIENGAGK"
     gene            3799..4311
                     /locus_tag="PLL88_10200"
     CDS             3799..4311
                     /locus_tag="PLL88_10200"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOJ01976.1"
                     /translation="MKKESQSQMGHVWAPWRMKYIQETSETGCFFCKGIREEDGDNNL
                     IVKRGKFAFVMLNRYPYTSGHLMVVPYAHEASMEALPIETKTEMMVLVTQAIKVLTKE
                     YGAEGFNIGANLGAIAGAGVADHLHFHVVPRWNGDTNFMTSVGNARVLPETLNDTFHR
                     IKDNWIIEAD"
     gene            4319..4807
                     /locus_tag="PLL88_10205"
     CDS             4319..4807
                     /locus_tag="PLL88_10205"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ01977.1"
                     /translation="MSAITIRNISPKDKTWIKTLMLESWGSEKVVVCGRSFEPASLTG
                     MIAELDRERCGLATYIISDITCEVITLNSLQSGLGVGSTLLDAVAQNAKEQGCKLITL
                     FTTNDNLNALRFYQKYGFHLAQLIPNGIETDRKIKPEIPLQGENGIPIRDYLELRMDL
                     DV"
     gene            4804..5667
                     /locus_tag="PLL88_10210"
     CDS             4804..5667
                     /locus_tag="PLL88_10210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOJ01978.1"
                     /translation="MKLRLKLAALAARILPPRWKTALYRSRKLSGLIRNNLNSAAPQG
                     MSTISIAGGMNKDMQLKLDMQTEKDYWLGTYEVDLQDAIKKYCERGMVVYDVGANIGY
                     ISLMFAKQSGETGRVFAFEPLPANCARLQENIELNDLTGRIHLTQAAVVDKPGKISFM
                     VHQSGAMGKAQGSLGRDEHYMRAIEVKAITLDDFIFQQGHVCPDLVKMDIEGGEVLAI
                     KGMQRTLRECNPIMLVEIHSHSALECLWEAFQKADYHLYKMEKAYPLIQDLMQLGEKT
                     YAVAIPNKKAG"
     gene            complement(5700..5894)
                     /locus_tag="PLL88_10215"
     CDS             complement(5700..5894)
                     /locus_tag="PLL88_10215"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOJ01979.1"
                     /translation="MSDNVHKLYRSRDSRMIAGVCGGLGEFMGVDPTIVRLLFVLGLF
                     LGTLTFWVYLVMMLVIPEEP"
     gene            complement(5960..>6304)
                     /locus_tag="PLL88_10220"
     CDS             complement(5960..>6304)
                     /locus_tag="PLL88_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOJ01980.1"
                     /translation="IAFEMLSSINFLEPALEIPYCHHEKWDGSGYPRGLKGEEIPLSA
                     RIFAIVDVWDALSSDRPYRKAWSRHKVIEYIKSQSGTHFDPRVVDAFIPLVQEKDQES
                     KPTEKKHKKTKS"
BASE COUNT         1719 a         1589 c         1583 g         1413 t
ORIGIN      
        1 cgagctgctg cataccctga ccgatacggc ggtattcacg gcatgataca ggacaaaaac
       61 atggaaaaca aaatcaattc caattcacca aaagccggca gagtgatcgg caccgtgatc
      121 ggcggcagcc tttctgaaag cctgagcgca cgtctgcaag tctcaccgca ggaaatccag
      181 gaaggcagtt tcgtgacgat caagagtggt gactggcaat tttacgggct gatcaccgat
      241 ctgcggttgg gtgccaccga cccgcgcttc tctgatgaac agatcgatgc gtactatccc
      301 gcagcgatcg cccgcgccct gcgtgaagaa gtgctgtatt ccaatgccgt gatcctgccg
      361 gtgctgatgc tggaaaacgg tccggaaatg aatgccgata acgcccagca agtgatgggc
      421 tggcagcaac aaaaggagga gaacggtgaa atgatgaccc gtcccatcca gatcaagacc
      481 atcccggcac accaatcggc agtgcgactg gcaaacgcgg tggacattgc caccgttttc
      541 ggcgatccaa gtgaggaaga caatttcgtg atcggctaca cacgcgaaca gaactttccg
      601 gtgtgcatcg atctggcaca tttcatccag cgttcggcag gcgtgttcgg tgccaccggc
      661 accggcaaaa gttttctcac gcgtattctg ctggcgggat tgatccagca agccaaagcc
      721 tgtctgctgg tcttcgatat gcatgatgaa tacggtttcg atgacacagc ttccgacagc
      781 ggtgaaaaag tgaccgggct gcacacgcat tttggaccga aagtgcaggt agtgggattg
      841 ggcggtggga agaacatccg tggcaacagc ccggattttc acgtagagat tgcggaaagc
      901 gatatcgaag ccagtgacat cgagatgctc tctgttgaac ttaacctgaa ggaaaccacc
      961 gccaccaccc tggcagcatt atcagccagt tttggtaaaa gatggttttc ggaattcaaa
     1021 gacatgaaag ccggcagcac cattgaagat gaaaatggga aaaaggttcc cgcgccggac
     1081 agtgtggttt ggtgggcgaa ccaaaatggt gtaaatgtga tggcagccga aagcttgcac
     1141 agcaagctgg gcagattgtt ccacgccggg tatatcaccg ccaagcctgc cggtaatgga
     1201 ttgcaaagtg tggtagacct gttgaagaat ggcaagcatg tcgttctctc cttcagcggt
     1261 tatgacaatg atttggatta tctgttcatt tcaaacctga ttacacgcaa gatccgcgaa
     1321 gaatgggaac agctcaccaa cgcttatcgc aatggcagga gcgatgccag tgaaccatgc
     1381 caactggtga tcgtggtgga ggaagcgcac aaattgctca accgcgaaat ggcatcgcaa
     1441 acgactttca gcaccattgc acgcgaaatg cgcaaatatt acgtgaccct gctgatcgtc
     1501 gatcaacgcc cctcgcagat ctatgatgag gtgctctcgc agctcggcac gcgcatctgt
     1561 ggttatctga cggacgacga agatatcagg gcggtgctct caggggcagc cggcaaagat
     1621 gccctgcgca gcatgctggc acacctgcag cccaaagaag aggtgctcat cgcaggctgg
     1681 ggagtaccca tgccgatccc ggtgcgctca cgacgattgg acgccggatt ctgggaagag
     1741 ttaaaaaaag gcagggcaag caccctgcac agtggtgaca tggaaacgca aaagaaaatg
     1801 ctgggtttct aaagaccgta atagatcacc cacatgatga gtgcgttaaa aatgccgagc
     1861 accacacccc cgatcgcttc aaagggagtg tgccccagaa cttcctgcaa tttctggtat
     1921 gaaaagctgc tgcttttgaa tatttcctcc acgatcttat tgatccattc agcatgaaag
     1981 ccagcctggc gacgaatgct gatcgcatcg taaatcacaa tggtagccag ggtcatggca
     2041 agggcaaaaa gagatgaccc gaacccttcc tgcaagccga tccccagagc agcagcagtg
     2101 gaaatggcag agtgcgaact gggcatgccg ccagggctga tcaagatcga ccaatcccac
     2161 tccttattga aaaaataatt gatcggcaat ttcaaaagtt gtgctacaag ccaggcagtg
     2221 gctgcactca tcagaacata attcgacaac aactgttgga aaatattcat ggctgatctt
     2281 cctggtcaga actcttctct tccaactgtc ctatttcacg cacacgcagt tcagcctggt
     2341 cgagcaactg ctggcaatgt gccaacagat cttttccctg ttcaaaacgc gccagcatct
     2401 catccagttc caatacaccg ctttccagat ggttgatcac ctgttctaac tgctgcaacg
     2461 cttcttcata tttcaatttt gagatatcga tttttgtgtt catgttattt tcctgctcca
     2521 ttttcgatag cagtaatgcg cgcttgcagt gatccgtctt tcaagcgcac gtgcaccatc
     2581 tcattttcgc cggttttggt gacactgtca atcaatttgc cctgaccatc gtatagcagg
     2641 gcataacccc gccgtagcac accctgcgga tcaagtgcca gaaggcgggc tcctttttct
     2701 ccaaaatgcg ctttgaactc agccagcctg gaataaaggg tatattgaag gcggcgcccc
     2761 acttcatcaa gctgcaacat attttgttgc aggagacgtt caggagaatt ttgcagcagg
     2821 cggtaataca gctctttcat ttcagcggtt ttcatctgca ccagctggct accaagcgat
     2881 accagcaacg attttctatc ttgcagatcg agcttgagat cctgaatatc gggaaccgcc
     2941 aataccactg ccccggtggg tgtgggggca cgcaggtctg ccgcgaaatc gaccagcgtg
     3001 aagtcggtct cgtgaccaac gccgctgata atgggaatgg gggatgccgc aaccgcgcgc
     3061 accactctct catcattgaa agaccacagg tcttccaatg agccgccccc ccgtgccagg
     3121 ataataacat ccggtctagt agaagcgttg tacagcaagt gcagtgcctg cacaatattg
     3181 tgcggtgcgc cttcgccttg caccaaggtc gctgaaagaa atacctgcgc cagcgggtag
     3241 cggctgcgca gggtgttgag aatatcctgc aaggctgccc cactggaaga agtgaccaca
     3301 ccaatcacag taggaaaagc aggcacgttt ttcttgagtt cttcggcgaa caatccttcg
     3361 gcttccaatt tattttttaa ttccaagaat tcctgaaaga gcctgccctc accctgctga
     3421 cgcatggaat caacatagaa ctggtactgc cctccttttt caaacacacc gaattttccg
     3481 tgcacatcca ccgccatgcc atcctgcggt cgaaagtgaa gggattgtgc catccatttc
     3541 cagataacac agcgcaggga ggccgcttca tctttaagcg tgaaataaat atgcccagaa
     3601 gccggagacg aaaggttgga tatctcaccc tgcacccaga tatcctgaag gtcttcattc
     3661 tcttcgataa gcacgcgaat atagccggta acttcactaa cggaaaatat ctggcgagaa
     3721 aaaagagatc ctgtaagcat gcaaatacca gtataatctc tttttcgtcg aaactgcgcg
     3781 tataataagg acttgaagat gaagaaggaa tcgcaatccc agatggggca cgtatgggct
     3841 ccatggcgca tgaaatatat tcaagaaacc tccgaaacag gatgtttttt ctgcaaaggc
     3901 atccgcgagg aggatggcga taataatctg atcgtaaaac gcggcaagtt cgctttcgtg
     3961 atgctcaatc gttacccata taccagcggg catttaatgg tagtacccta cgcgcatgaa
     4021 gcttccatgg aagctttgcc aatcgaaaca aaaaccgaaa tgatggttct ggtaacccag
     4081 gcgatcaagg tgctcaccaa ggaatatggc gcagagggct tcaacatagg cgccaatctg
     4141 ggagccattg cgggagccgg agtggcagat cacctgcatt ttcatgtggt gccacgctgg
     4201 aatggggaca ccaatttcat gacctccgtt ggaaatgcgc gcgttctacc cgaaacactc
     4261 aatgatacgt ttcatcgtat caaggataac tggatcatag aagcggattg accttctgat
     4321 gagcgcgatc actatccgaa acatttctcc caaagataaa acctggatca aaaccctgat
     4381 gttggaaagc tggggaagtg aaaaagtggt agtgtgcgga cgttcttttg aacctgccag
     4441 cctgactggc atgatcgcag agctggatcg ggaacgctgc gggttggcaa cctatatcat
     4501 cagcgatatc acgtgtgagg tcatcacgtt gaacagtctg caaagtggat tgggtgtggg
     4561 cagtacattg ttggatgcgg tagcgcaaaa tgccaaagag caaggctgca aactcatcac
     4621 gctcttcacc accaacgaca acctcaatgc tttacgcttt tatcaaaaat acggatttca
     4681 cctggcacaa ctcatcccca acgggatcga aaccgatcgc aagatcaagc ccgaaattcc
     4741 cctgcagggg gaaaacggca tcccaatcag ggattatctt gaattgagaa tggacctgga
     4801 cgtatgaagc tacgactcaa gctggctgcc ttggctgccc gtattctacc cccgcgctgg
     4861 aagactgctt tatatcgttc ccgcaagctg tctggactca tccgcaacaa tctgaacagt
     4921 gctgccccgc aggggatgag caccatttcg atcgccggag gaatgaacaa agatatgcaa
     4981 ttgaaactgg atatgcaaac agaaaaagat tactggctgg gaacctatga ggttgacctg
     5041 caggatgcta tcaaaaagta ttgtgagcgc ggaatggtgg tttatgacgt gggagccaac
     5101 attggctaca tcagcctgat gtttgccaaa caaagtggag aaaccggacg ggtatttgcc
     5161 ttcgaaccgc tgcccgccaa ctgtgcacgc ttacaggaaa acatcgaact gaatgatctg
     5221 accggtcgca ttcacctgac ccaagccgct gtagtagata aacctggcaa gatatctttc
     5281 atggtacatc aatccggtgc aatgggaaaa gcacagggtt cactggggag agatgaacac
     5341 tatatgcgtg cgatcgaggt aaaagccatc acactggatg atttcatttt tcagcaggga
     5401 catgtttgcc ccgacctggt caaaatggat atcgaaggtg gagaggtact ggcgatcaag
     5461 ggtatgcaac ggacactgcg ggagtgcaat cccatcatgc tggtggagat ccacagccac
     5521 agcgccctgg agtgcctgtg ggaggctttt cagaaggcag actaccactt atataaaatg
     5581 gaaaaagcct atcctctcat ccaagacctg atgcaactag gtgaaaaaac gtacgccgtg
     5641 gcaattccta acaaaaaagc cggttgacaa aaccggcatt tcatttaaaa cagcggcact
     5701 tatggttctt cggggatgac cagcatcatg accagataga cccagaaggt cagggtgcca
     5761 agaaataaac ccagcacaaa aagcaaccgt acgatggtgg gatcgacacc catgaattct
     5821 cccaagccac cacacacacc ggcgatcatg cggctatccc gtgatcgata aagtttatgg
     5881 acgttatcag acattggtat tctccttatt cattgtggct tacaacttat atacgtgcca
     5941 tgccataaaa ggttgcaatt caagatttag tttttttatg tttcttttca gtaggcttgc
     6001 tttcctgatc tttttcctga acaaggggaa taaaagcatc gacgaccctg gggtcgaaat
     6061 gagtgccaga ttgtgatttg atatattcaa tcactttatg gcgagaccag gctttccgat
     6121 aagggcgatc ggaagaaagc gcgtcccaca catctacaat ggcaaatatc ctagcggaca
     6181 gcggaatctc ctcgcctttc aatccacggg gataaccact gccatcccat ttttcatgat
     6241 gacagtaggg tatctcgagc gccggctcga ggaaattgat ggaagaaagc atctcaaaag
     6301 caat
//
LOCUS       DAOSDS010000115         6275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4488_length_6275_cov_4.515595, whole genome shotgun sequence.
ACCESSION   DAOSDS010000115 DAOSDS010000000
VERSION     DAOSDS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6275
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4488_length_6275_cov_4.515595"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..1409
                     /locus_tag="PLL88_10225"
     CDS             150..1409
                     /locus_tag="PLL88_10225"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01981.1"
                     /translation="MKKIFTIAWKDLLRSIRSLYFIGISLIAPLLLSFLFSAAFGGGG
                     ESTAISNIAIAFVNQDVPPQGSPNLGETVYEIFHSAGLKDLITLHEVAGEEEALQGIN
                     EQDFSAAVIVPADFSQVVSGQGGTTHLRVIYDPAENIAPQVLESITATIANEFSSSKI
                     MVSLAVEELQRRGGQASEQFYTDLVARITARSESQSGVTSPLLTVLNPQGQKEEARDE
                     MQQIISIIMVGMMIFFAFYTGAATSESLLQEEEKGTLARKFVSPTPVRDILAGKFLGV
                     LITLVVQICVLLVLSSLVFHIQWGEAGKFILAVIALIAAAAGFGLLLMSFLSDSRQTG
                     IILGGVMTITGMVGGLFTSTIQNMPAFLGILKKFTPQGWALDLWQLVLNGAGYAEILF
                     PFSVLLCMAVVFFIIANFRFQRRFAKK"
     gene            1419..2648
                     /locus_tag="PLL88_10230"
     CDS             1419..2648
                     /locus_tag="PLL88_10230"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ01982.1"
                     /translation="MKRIFEIVWKDISQTLRSPMAALFLLALPLMFTAFFGMIFQGVS
                     DEDAALDKPVVALVGEEESTAILPQLQSLLTETGAVSVEVFGSNEFPDPLALFEAEEV
                     DAVLYLPEHFRFSLQDSAPAILYVDENSSKGQTVLYTIERVFSQEQALYSTSAAAVQT
                     VDEQLPYASDQEYQMAFEQVLAKANQAWSQRAVTVSMERAVTATTDLSRIESAYDQSS
                     PGMLVQFTISGLMGTAVIFVQERRTKTLQRMLTTTTSRSSIIIAHALSMLFIIVVQEI
                     ILVLSGQFLFGVDFLREPFAILLVILAFALWVSGMGILIGVLAKNEDQVVLFSLIAMF
                     LFTAMGGAWFPLEGTSPAFYNIARYTPGAQAMQGFQNVIVRKQGFSSVLEPVGILLLY
                     AVLFFSAAILIFKKNKE"
     gene            2738..4285
                     /gene="ftcD"
                     /locus_tag="PLL88_10235"
     CDS             2738..4285
                     /gene="ftcD"
                     /locus_tag="PLL88_10235"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOJ01983.1"
                     /translation="MSKKLVECIPNFSEARRPEVVEQIAQAILSISGVQILDRHSDQD
                     HNRTVITILGEPHAVEEAIFAAIKRAAALIDMDQHQGEHPRIGATDVVPFVPIRGMEM
                     SECVEIARHLAKRVGEELSIPVYLYEEAAVRAGWANLEDIRRGQYEGLKEEVKTNLER
                     KPDFGPAELGKAGATVIGARYPLIAFNVYLTTDDVNIAKKIARQVRHSSGGLRFVKAM
                     GVLVEGRAQVSMNLTHFQKTPLAQVVEFVRREAQRYGVAIHHSEVVGLIPQQALTDAA
                     AWYMQLDQYDDEQLLENRLMSAATEPSGDSGEKSTSFLDELASANPTPGGGSAAAWAV
                     AEAAALVAMVARLTVGKSKYKEVESLMFSLVEQAEELRAKLQQAVREDAAAFEKVMAA
                     LKLPKDTEEQITLRQNAIQIATLEAARVPLDTARMALAVMQLAETVCKAGNLNAITDA
                     ATAVQLAAAGLASSAANVSINITGLDDENTKHEFEREIGSLKREKDAILSRVAQSVMD
                     RSQIALI"
     gene            4300..5952
                     /locus_tag="PLL88_10240"
     CDS             4300..5952
                     /locus_tag="PLL88_10240"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOJ01984.1"
                     /translation="MKRLSVVTLILFCIAMGMSACSFQTRSGATIEPSVQVIATETPL
                     PANQVYSMWLAPYLPQGLQTQIALPRNVTLVDRQEEADVWLDIGSDQPISQWVYALAA
                     PFPTVADEISFQELLQVWNGQVPADSPLQKILVDGKTKAIFEKVWGMPSSSSVVLTSE
                     ETLLTAAWGETGAWAMIPFEMLETRWKVIAVNGISPIDDDFNSEAYALSVSFSLFGKQ
                     EDLLAVNERIAASESALLPLTNRDENKITTVVVTGVTALTRGTAFMMEKYGMTYPALD
                     IGDVLRGADITHISNEIPFTTTCPNPFYNAENDANLVFCSKPEYINLLESVGTDVVEL
                     TGDHFRDWGADAMLYTLDMYDERGWEYYGGGRNIEDGQQPALFEHNGNKIAFLGCNAK
                     PEGYATADEDSPGAVHCDLQVIAAKIKEVKEQGYIPIVTFQHVEYYAYTASPYLVNDF
                     HAVVEAGAAIVSGSQAHQPHAFEFYKGAFLHYGLGNLFFDQYTEGAAQRQAFIDRYII
                     YDGRVISTDLITIQFIDLARSQFMTSEERQALLRTVFAASGW"
     gene            5974..>6275
                     /locus_tag="PLL88_10245"
     CDS             5974..>6275
                     /locus_tag="PLL88_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HOJ01985.1"
                     /translation="MMGILPDYKIREYAKKQAMIEPFVDGQVRDGKISYGLSSYGYDI
                     RVADEFKIFTNVHTTLVDPKDFDTRSMVDFKGDVCIIPPNSFALARTVEYFHIPRGV"
BASE COUNT         1523 a         1444 c         1697 g         1611 t
ORIGIN      
        1 gtcagtgaca gcaacgcggt attgcccgag cttttcaaga ttgctcatca acaaaatgcc
       61 cgcatcgcct ccatagcaat tcatgagccc aacctggaaa cggtgtttct gcacctgacc
      121 gggaaagcgt tgcgcgatta gaggggaaca tgaaaaaaat atttacgatc gcctggaaag
      181 atctgctgcg ttcgatacgc agcctgtatt ttattggaat atctctgatt gcgccgctgc
      241 tgctctcttt tcttttttct gcagcctttg ggggcggggg tgagagcact gccatttcaa
      301 atattgctat cgcttttgta aaccaggatg ttccccctca aggcagtccg aacctgggtg
      361 aaactgtgta tgaaattttc cattcagcag ggttgaaaga tctgatcact ttgcatgagg
      421 ttgccggtga agaagaggca ttgcagggga tcaacgaaca ggatttcagt gcggcagtta
      481 tcgttccggc tgatttttct caggtcgtga gcgggcaagg aggaacaacc catctgcggg
      541 tcatctacga cccggctgag aatattgctc cccaggttct tgaaagtatc actgctacca
      601 ttgcgaatga attttcttct tcaaagatca tggtctcttt ggcagtggaa gaattgcaac
      661 gccgcggagg gcaagccagt gaacaattct atacggattt ggtggctcgt atcactgcgc
      721 ggtcagagag ccaatctggc gtaacttctc ccctgctgac cgtcttgaat ccgcaggggc
      781 agaaggagga agccagggat gaaatgcagc agatcatttc catcattatg gtggggatga
      841 tgatcttttt cgctttttat accggtgccg caacgtcaga gtctttgctg caagaagaag
      901 aaaaaggcac cctggcacga aaatttgttt cacccacacc ggtgagagac attctggcgg
      961 gcaaattcct gggcgttttg atcaccctgg tcgttcaaat ctgtgtgctc ttggtcttat
     1021 ccagtcttgt ttttcacatt cagtggggag aagcgggaaa attcattctg gctgtcattg
     1081 cgctcatcgc tgccgcagcc ggttttggac tgctgctcat gtcgtttttg agcgattccc
     1141 gccagaccgg tatcattttg ggcggcgtta tgaccataac cggcatggtg gggggattat
     1201 tcaccagcac catccaaaat atgcccgctt ttttgggaat attgaaaaaa tttactcccc
     1261 aaggctgggc attggatctc tggcaattgg tattgaacgg agctggttat gcagagattt
     1321 tgttcccatt ttctgttttg ctctgcatgg cagtggtttt cttcatcatt gccaatttcc
     1381 gctttcaaag acgttttgcg aaaaaatagg atgaagagat gaaaagaatt tttgaaatcg
     1441 tttggaaaga tatttcccag acattgcgca gtcccatggc tgccttgttc ttgcttgcac
     1501 taccgctgat gttcaccgct tttttcggaa tgatctttca aggtgtgtcg gatgaagatg
     1561 cagcgctgga taagccggtt gtagcgttgg tgggcgaaga ggagagcaca gcgatcctgc
     1621 cccaactgca atctcttctc acagaaaccg gcgcggtgag cgttgaagtg tttgggtcaa
     1681 atgaatttcc cgacccgctt gcgcttttcg aagcggaaga ggtggacgct gttttgtatc
     1741 tgccggaaca ttttcgcttt tctctccaag attcagcccc ggcgatctta tatgtggatg
     1801 agaacagtag caaagggcaa accgttctct acacgattga acgggttttc agtcaggaac
     1861 aagctctgta ttccacttct gcagcagcag ttcaaactgt cgacgagcaa ctgccgtatg
     1921 ccagcgatca agaataccag atggcgtttg aacaggtatt ggcaaaggca aaccaggctt
     1981 ggagccaaag ggcagttacc gtttccatgg aaagagctgt cactgccacc acggatctct
     2041 cacgcatcga gtcagcctat gatcaatctt cccctggcat gctggtacaa ttcaccatca
     2101 gcggattgat gggcactgcc gtcattttcg tgcaggaacg gcgaacgaaa acgttgcagc
     2161 ggatgttgac gactacgacc agccgctctt cgatcattat tgcccatgca ctttctatgc
     2221 tctttatcat tgttgtgcag gagatcatct tagtgttgag cggacaattc ctgttcgggg
     2281 ttgatttttt gcgggaaccc ttcgcgatct tgctggtgat ccttgccttt gccctgtggg
     2341 tgagtgggat gggcatcttg atcggggtat tggcgaaaaa tgaggaccag gttgttttat
     2401 tttctttaat tgccatgttc ctcttcactg ccatgggcgg tgcttggttc cctttggaag
     2461 ggaccagccc cgcgttctat aacattgccc gttatacgcc cggagctcaa gccatgcagg
     2521 gttttcaaaa tgtcattgtg cgcaaacagg gattctcttc ggttcttgag ccggtcggta
     2581 tattgctgct gtatgcggtc ttattctttt cagcagctat ccttatcttt aaaaagaaca
     2641 aagaataaga aaaagaagaa aatcgagccc gtatgcgaca tacgggcttt tttcaattaa
     2701 taacggggtt tataatcttt caagacaagg aattcacatg agcaaaaaac tggttgaatg
     2761 tatcccgaat ttttctgaag ctcgccgtcc ggaagtagta gagcagatcg cgcaggcgat
     2821 cctttccatt tccggagtgc agatcttgga tcgccactca gatcaagatc acaaccgaac
     2881 ggtgatcaca atcttggggg agccgcacgc tgtggaagag gcgattttcg cagctattaa
     2941 acgagcggct gccctgatcg atatggacca gcatcaaggc gaacaccccc gcatcggagc
     3001 aacggatgtg gtaccctttg tgcccatccg tggtatggaa atgagcgaat gtgtggagat
     3061 tgcccgccat ctggcgaaac gggtggggga ggagctttcc atcccggtgt atttgtatga
     3121 agaggctgca gtgcgagccg ggtgggcgaa cctcgaagat atccggcgtg ggcagtatga
     3181 aggattgaag gaagaggtga aaaccaatct ggagcggaaa cccgattttg gacccgctga
     3241 acttggtaaa gccggagcaa ccgtgatcgg cgcacgctat ccgttgattg ctttcaatgt
     3301 atacctgacg acggatgatg ttaacattgc aaagaagatc gccaggcagg tgcggcattc
     3361 ttcgggtgga ttgcgttttg taaaagcgat gggggtgctg gtggaggggc gggcgcaggt
     3421 ttccatgaac cttacccatt tccaaaaaac gccccttgcc caggtggttg aatttgtgcg
     3481 gcgcgaagcg cagcgttatg gagtggcaat tcatcacagc gaagtggtag ggttaatccc
     3541 gcaacaggcg ctgaccgatg cggcagcctg gtatatgcaa ttggaccagt acgatgatga
     3601 acagcttctt gagaacaggc tgatgtctgc cgcaacggaa ccatcaggcg attctggaga
     3661 gaaaagtact tcttttttgg acgaactcgc cagtgcgaac cccactccgg ggggtggttc
     3721 agccgccgcc tgggctgtag ctgaagcagc cgccttggta gccatggtag cccgtttgac
     3781 ggttggcaag agcaaatata aagaggtcga gtcgctcatg ttttcactgg ttgagcaggc
     3841 ggaagaattg cgggcaaagt tgcagcaggc ggtgcgagaa gacgcagccg ctttcgaaaa
     3901 ggttatggct gccctgaagc tacccaaaga tacggaagag cagatcacct tgcgtcagaa
     3961 cgccatccag atagcaaccc tggaagcagc tcgggttcca cttgatacag ccagaatggc
     4021 attggcggtc atgcaacttg cggaaactgt ttgcaaggcg ggcaatctca atgcgatcac
     4081 cgacgctgcc actgcagttc aactggcggc tgccggtttg gccagttcag ccgccaatgt
     4141 cagcatcaat atcaccgggt tggatgacga aaacacaaaa cacgaatttg agcgagagat
     4201 aggatcccta aagcgggaaa aagacgctat acttagccgg gtggcgcaga gcgttatgga
     4261 tcgttcacag atcgcgctga tctaaggatt gttgcttcaa tgaaaagact ttccgttgta
     4321 actcttatct tgttctgcat tgctatgggt atgagcgcct gttctttcca aaccagatct
     4381 ggcgcaacaa tcgagccctc cgtccaggtc attgccacag aaacccccct ccctgccaac
     4441 caggtatatt ctatgtggct tgcgccatat ttgccgcagg gtttgcaaac tcagattgcg
     4501 ctacccagaa acgtcaccct ggttgatagg caggaagaag ccgatgtctg gctggatatc
     4561 ggcagtgacc aaccgatcag ccagtgggtg tatgcgctgg ctgctccatt ccccacggtg
     4621 gcggatgaaa tctctttcca ggaattgtta caggtttgga atgggcaagt tcctgcagat
     4681 tccccattac aaaagatact ggtggatgga aagaccaagg ctatttttga aaaagtttgg
     4741 ggcatgcctt ccagttcgag tgtggttctc accagtgagg aaaccctgtt gactgctgca
     4801 tggggcgaaa caggcgcctg ggcgatgatc ccttttgaaa tgctggaaac gcgttggaag
     4861 gtcatcgcgg taaacggtat ttctccgatc gatgatgatt tcaattctga agcgtacgct
     4921 ttaagcgttt ctttttcgct ctttggaaag caagaagatc tgctggctgt caatgagcgt
     4981 attgcagcct ctgagagcgc gctgctccct ttaaccaatc gagatgaaaa taagatcaca
     5041 acggtggtcg tgacgggtgt gacagctttg acgcgcggga ctgcctttat gatggaaaaa
     5101 tatggcatga cttaccccgc cttggatatt ggggatgtct tgcgtggcgc ggatattacc
     5161 catatcagca atgaaatacc gttcaccaca acctgcccca accccttcta caatgcggaa
     5221 aatgatgcca acctggtttt ttgcagcaaa cctgaataca tcaacttgct ggaatccgtg
     5281 ggaacggatg tggtggagtt gactggtgat catttccgcg attggggcgc ggatgccatg
     5341 ttgtataccc tggatatgta cgatgagcgc ggatgggaat attatggggg tgggcggaat
     5401 atcgaggatg ggcagcaacc cgcccttttt gaacacaacg gtaacaagat cgcatttctt
     5461 ggttgcaacg ccaaaccgga aggatatgcc acggcggatg aggattcacc gggagctgta
     5521 cattgcgatc tgcaggtgat agctgcaaaa atcaaggaag ttaaagagca gggctacatc
     5581 cccatcgtaa cctttcagca tgttgaatac tacgcgtaca cagccagccc ttacctggtg
     5641 aacgatttcc atgctgtagt agaagctggg gcggcgattg taagtggaag tcaggcgcac
     5701 cagccccatg cttttgaatt ctataaaggc gcgtttttac attatggttt gggaaacctt
     5761 ttttttgacc agtataccga gggcgcagct caacggcagg ctttcattga tcggtatatt
     5821 atttacgatg gacgggtgat cagtaccgac ttgatcacga tacaatttat cgatttagcc
     5881 agatcgcagt tcatgacctc agaagaacgc caggcgttat taaggaccgt gttcgcagcc
     5941 agcggatggt aacgaaaaac agaacaagga gcaatgatgg gcatcttacc agattataaa
     6001 atacgggaat atgcaaaaaa gcaggcgatg attgaaccgt ttgtggatgg gcaggtgaga
     6061 gatggcaaga tctcttatgg tctttcttcg tatggttatg atattcgcgt ggcagatgaa
     6121 tttaaaatat ttaccaatgt tcataccacg cttgtggatc ccaaggattt tgatacccgc
     6181 tcgatggtcg atttcaaggg cgatgtgtgt atcattccgc ccaattcctt tgcgctggcg
     6241 cgcacggtgg aatactttca tatcccgcgc ggggt
//
LOCUS       DAOSDS010000116         6228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4523_length_6228_cov_4.463146, whole genome shotgun sequence.
ACCESSION   DAOSDS010000116 DAOSDS010000000
VERSION     DAOSDS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4523_length_6228_cov_4.463146"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PLL88_10250"
     CDS             <1..594
                     /locus_tag="PLL88_10250"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOJ01986.1"
                     /translation="ERDFYLQPVVDVARSLIGKRLVRNLHDDLLAGIIIETEAYDGEQ
                     DRACHAHRGKTRRNAVMYGPPGCAYVYFTYGLHWLLNCVTGAEDYPAAVLIRAIQPIH
                     GLEVISQNRAGIAQELWCNGPAKLTRALAIDGTFNGCDLCQQNGMLQITEGITIPDNK
                     IQATPRIGIGYAGEPWISKPWRFEAKMQLAEVNALIE"
     gene            642..1322
                     /locus_tag="PLL88_10255"
     CDS             642..1322
                     /locus_tag="PLL88_10255"
                     /inference="COORDINATES: protein motif:HMM:NF036822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ01987.1"
                     /translation="MPVFDFDPTKVIIYGGGGLSKMIIETVRVLGTYQIEGVIDDHMQ
                     KGTDVIGSPVLGGAEVLPELAARGIRMAVNSVGGIGDFQVRLNVFHTLAKAGFLCPAV
                     VHPTAHIDPSAQLAAGVLVLAQSYISGNARVGMGTLINNSVVVSHDNVLGICVNLSPG
                     AMLAGDVVIDDFAQLGMNVNVNIGVHIGKYARIANGATIKGDVPQGGRVYAGTVWPPR
                     DLPKIRAA"
     gene            1336..1773
                     /locus_tag="PLL88_10260"
     CDS             1336..1773
                     /locus_tag="PLL88_10260"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOJ01988.1"
                     /translation="MFRKNAANARPEITPIERVTSVLGAGIIWKGSIKGKGGVRVEGT
                     FEGEINLNGLLVVGETGRITCENVQANTVIVAGAIKGNITAEKLEIRSTGHVWGDVKI
                     VAFSTEEGAFLRGNIIMEEQVALDFEETPAEAKTPGQEPQIEK"
     gene            1794..3542
                     /locus_tag="PLL88_10265"
     CDS             1794..3542
                     /locus_tag="PLL88_10265"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOJ01989.1"
                     /translation="MRISQLFHQTLRRSSDQKDSAGHQFLMQAGYIRPLASGIFGYLP
                     LAQRTLSKISEIIRQEMDAIGGQEVSLPLVHPAEIWQETGRWYSINEEMGRFQDRNGR
                     DMVLAMTHEEAVAAVTRDVVQSYRQLPALIYQLQIKWRDDPRPRAGMIRAREFTMLDS
                     YSLDKNEDGLEKQYQAHFHAYTRIFERCNLPVMAIKSDTGMMGGTYAHEFMYLTPIGE
                     DTIILCNSCHYAANRQIARSQKSSPTNEELKEINKVATPNKKTIAELVEYLNIPAQKT
                     AKAVFIMAERKDGWQLVLALLRGDLELNETKLSHILRARQLRPAMDDEIKASGAVPGY
                     ASPIGLKNILVVADDEIPDLRNLAAGANETGYHLLNVNYGRDFKADIIADIACADQGL
                     PCPECGKTLTAQRGVEVGNIFKLGTFYSEHMGCNFLDEDGTSKPIVMGSYGIGLGRLM
                     TCIAEEHHDAKGLTWPAHLAPFPVYLIHLEDKSGETRQIADSLYTTLQNSGLQTLYDD
                     REVSPGVKFNDADLLGIPFRLTVSSRSLSNGGVEIKSRTDEKSQIKEAAQIMELLLAA
                     RHENIVSLHNTSVQGK"
     gene            3543..4727
                     /locus_tag="PLL88_10270"
     CDS             3543..4727
                     /locus_tag="PLL88_10270"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ01990.1"
                     /translation="MNKKHENTVPMSSPEINDDDRQAVLEVLNTTSLSMGPKIDQFER
                     SIRQRTGSRYALGVSSGTSGLHLCVRAAGIEQNDLVITTPFSFISSTNVILFEKATPV
                     FVDVDLASGNLNTAQLQQAVYDIRQGGKSAARWLPRKNHAEYQKLKAVLSVDVFGQPA
                     DYDIIRKICDDNQLVLIEDSCEALGAEYKGKQAGTFGNYGVFAFYPNKQITTGEGGVI
                     VTDDEAAAAYMQALRNQGRAPGDAWLQHTYLGYNYRLDEMSAALGYSQMSRLDHMLDC
                     RARVAAWYQDYLQEFSQIETPHILPTTTRMSWFVYVIRVSGSMDRDAFAKRLIQNGID
                     VRPYFLPIHLQPYIADQFGYRPGDFPVTEDLGNRGLALPFSSVMTEEEVLQVCEGIRR
                     SL"
     gene            4729..5616
                     /locus_tag="PLL88_10275"
     CDS             4729..5616
                     /locus_tag="PLL88_10275"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HOJ01991.1"
                     /translation="MDGEQIYRLPIRRTIANSLVFGILCIGLSVGSALITSSMQNEGF
                     RILGYILAILFAIPAIFFIFQIIRIARSRYAINRDGLTIYWGFQKMVIPIQEIEWIRP
                     YDQMGYAVPFPALGKIGMLSGHIFYQDLGDILFFATSHENAFLIGTSREVLFLSPMDA
                     QQFQKGIQEAVYSGSITPLERKSIQMESPVNTVRANPALYIPLVVGIFLALLLFVLFG
                     FIVNTHDTMQVGLIFFESTASVVIIPILAVIINGLNAFFAPRMAKNDTLKPYGILLAF
                     TGLLTTLFLIITLVLGLFS"
     gene            5991..>6228
                     /locus_tag="PLL88_10280"
     CDS             5991..>6228
                     /locus_tag="PLL88_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOJ01992.1"
                     /translation="MKSDDKLEKLKAMRNEAFVGGGEDRIAKQHEKGKMTARERLDRL
                     LDSGSFHEIGQFITPRNDSTAQGSDKTLGDGVVTG"
BASE COUNT         1694 a         1513 c         1553 g         1468 t
ORIGIN      
        1 gaacgagatt tttacctgca accggtagta gatgttgccc gatccttgat cggaaagcga
       61 cttgtgcgta atctccacga cgatctgctg gcagggatca tcatagaaac ggaagcctat
      121 gatggagaac aggatcgagc ctgtcatgcc cacagaggga aaaccaggcg aaatgccgtg
      181 atgtacggac cccctggttg cgcttatgtc tatttcacct atggtttgca ctggcttctg
      241 aattgcgtga ccggtgccga agactaccct gccgccgtac tgatccgtgc tatccaaccc
      301 atccatggct tggaggtcat ctcgcaaaac agagcgggaa tcgcgcagga gctttggtgc
      361 aacggaccgg caaagcttac ccgcgccctt gctatcgatg gcacgtttaa cggttgcgac
      421 ctctgccaac aaaatggaat gttacagatc acagaaggga ttacaatacc agacaacaaa
      481 attcaggcga cccctcgtat cggcattgga tacgctggag aaccctggat cagcaagccc
      541 tggcgttttg aagccaaaat gcaactggca gaagtgaacg cgttaatcga atagacacat
      601 ggtataaaaa gactatgcaa gaaacaataa atgagataaa agtgcctgta tttgattttg
      661 acccaaccaa agtgatcatc tatggcgggg gtggtttgag caagatgatc atcgaaactg
      721 tgcgggtctt gggaacctac cagatcgagg gcgtcattga tgatcatatg caaaaaggga
      781 cagatgtgat cggctcccct gtgctgggag gggctgaagt gcttccggag cttgccgctc
      841 gcggcatccg catggcagtc aactcggtcg gcggaattgg cgattttcag gttcgcttga
      901 acgtttttca caccctcgca aaagcgggct ttctctgccc cgccgtcgtt catccaacag
      961 cgcacataga tccttcggca caattggcag caggggtatt ggtgctggca caaagttaca
     1021 tcagtgggaa cgcccgggta ggcatgggaa cattgatcaa caattcggtg gttgtatcgc
     1081 atgacaacgt gctcggaatc tgcgtcaatc tatcgcccgg ggcaatgctg gctggggatg
     1141 tggttatcga tgatttcgcc cagttaggca tgaacgtgaa tgtgaacatt ggtgtacaca
     1201 tcggcaaata cgcccgtatt gccaacggcg ccacgatcaa aggagacgtt ccgcaaggtg
     1261 ggcgagtgta cgctggcacg gtttggcccc ccagagatct accgaaaata agggctgctt
     1321 aggagatatg gaagcatgtt ccgtaaaaat gccgctaacg ctcgtccaga aataacccct
     1381 attgaacgtg tgacttccgt gttgggagcc gggatcatat ggaagggctc catcaaaggg
     1441 aagggggggg tacgggtaga gggcacgttt gaaggcgaga ttaacttaaa tggcttactg
     1501 gtggtcggag agaccgggcg gatcacctgc gaaaacgttc aagccaacac ggtgatcgta
     1561 gcaggtgcca tcaaaggcaa tatcaccgct gaaaaactag agatacgcag caccggtcat
     1621 gtgtggggag atgtcaagat cgtggcattt tcaaccgaag aaggagcttt tctgcgtgga
     1681 aacatcatca tggaagaaca ggtggcattg gatttcgaag aaacacccgc agaagccaag
     1741 acccctggtc aagagccaca gattgaaaaa taaaagctct tttcaatacc atcatgagaa
     1801 tatcccaatt atttcatcaa acactgcgca ggtcatctga tcagaaagat agcgctggtc
     1861 accagttcct catgcaagct ggttatatcc gccctctggc tagtggcata ttcggctact
     1921 tgcctttggc acaacgcaca ctttccaaaa tttctgaaat cattcgccag gaaatggatg
     1981 ccattggggg gcaggaagta tcattgcccc ttgtgcaccc ggcggagata tggcaagaaa
     2041 ccggacgttg gtattccatc aacgaagaaa tgggcaggtt tcaagatcgc aacggtcgag
     2101 atatggtgct ggctatgacc catgaagaag ctgtagccgc ggtaacccgc gacgtcgtcc
     2161 agtcttaccg ccagctcccc gctttgatct atcagttgca gattaaatgg cgcgacgacc
     2221 ctcgcccacg ggcagggatg atccgcgcgc gcgaattcac catgctcgac agctatagcc
     2281 tcgataaaaa tgaggatggg ctagaaaagc aatatcaagc gcattttcat gcctataccc
     2341 gcatctttga acgctgcaac ttgcccgtga tggcaatcaa atcagacacc ggcatgatgg
     2401 gaggcaccta cgctcatgaa ttcatgtacc tgacccccat cggtgaagac acgatcattt
     2461 tatgcaattc ctgtcattat gcagccaacc gccagattgc cagatcccaa aaatcttcac
     2521 ccacaaacga agagttgaag gaaataaaca aagttgctac cccgaacaag aaaaccatcg
     2581 cagagctggt ggaatatctc aatatccccg cacaaaaaac cgctaaagca gtgttcatca
     2641 tggcggagcg caaagatggc tggcaactcg ttttggcact tttacggggt gatctggaat
     2701 tgaacgaaac caaactcagc catattctcc gcgccaggca gttgcgtcct gccatggatg
     2761 atgagatcaa agccagtgga gccgttccgg gctatgcctc cccgatcgga ctgaagaata
     2821 ttctggtagt ggcggatgat gaaatccctg atttgcgaaa cctggctgcg ggagccaacg
     2881 aaacaggata ccacttgttg aatgtaaatt acggcagaga cttcaaagct gatatcatcg
     2941 ctgatatcgc ctgcgcagac caggggctgc cctgccctga atgtggaaaa accctcaccg
     3001 cccagcgcgg cgtagaagtt gggaacatat tcaagctggg cactttctat tcagaacaca
     3061 tggggtgcaa ttttctggat gaagatggaa cctcaaaacc gatcgtgatg ggctcttatg
     3121 gaatcgggct gggaagactg atgacctgca ttgccgaaga gcatcatgac gctaaggggc
     3181 tgacatggcc agcacacctt gccccttttc cagtttacct cattcatctt gaagataaga
     3241 gcggagagac ccgccaaatt gcagactcgc tttacacaac acttcaaaat tctggtctac
     3301 aaaccttata cgacgaccgc gaagttagcc cgggtgtaaa attcaatgac gctgacctgc
     3361 tgggtatccc ttttcgtttg accgtcagtt ccagatcttt gtcaaatggt ggagtcgaaa
     3421 taaaatccag gacggatgag aaatcgcaga tcaaggaagc agcccaaatc atggaacttt
     3481 tgttagctgc ccggcacgaa aacatcgtta gcctgcacaa cacatctgta caaggaaaat
     3541 gaatgaataa aaaacatgag aacactgttc caatgtcatc ccccgagatc aacgacgatg
     3601 accgtcaggc ggttctggag gttttgaaca ccacatccct cagtatggga ccaaagatcg
     3661 atcaattcga gcgatcgatt cggcaaagaa ccggaagccg gtatgctctg ggcgtcagct
     3721 caggcaccag cggtcttcat ctgtgcgttc gcgcggcagg catagaacag aacgatctgg
     3781 tcatcaccac tcccttctcg tttatctctt ccaccaatgt gattcttttt gaaaaagcca
     3841 cccctgtttt cgtggatgtt gatcttgcca gtggaaattt aaacacagcg caactccaac
     3901 aggcagttta tgatataaga caaggcggaa aatctgctgc ccgatggttg ccgagaaaaa
     3961 atcatgcaga gtatcagaaa ctcaaagcgg ttctgagtgt agatgtattc ggtcaaccgg
     4021 cagattatga catcatcaga aaaatctgcg atgataacca gcttgtatta atcgaagatt
     4081 cctgtgaagc gttgggagca gaatacaaag gcaaacaggc tggcacgttc ggcaattatg
     4141 gggtcttcgc tttctatccg aacaaacaga tcaccaccgg agaaggtgga gtaatcgtca
     4201 cagacgatga agccgctgct gcctatatgc aagccttacg caaccagggg cgtgccccgg
     4261 gtgatgcatg gcttcaacac acgtatcttg gttacaacta ccggcttgat gaaatgagcg
     4321 ccgcgttagg gtattcccag atgagccgtt tggatcatat gctcgattgc cgcgcaagag
     4381 ttgctgcctg gtaccaggat tatttacagg aattctcaca gatcgaaacg ccgcacatct
     4441 tacccaccac aacccgcatg agctggttcg tctatgtgat cagagtgagc ggctctatgg
     4501 atcgtgacgc attcgctaaa cgcttgatcc agaacggaat cgatgtaaga ccttactttc
     4561 ttccaattca tctgcaacca tacattgccg atcaattcgg ttacagaccc ggtgactttc
     4621 ctgtcacgga agacctgggg aatcgcgggt tggcacttcc tttctccagc gttatgacag
     4681 aagaagaggt actgcaagtt tgcgaaggga tccgaagatc gttgtagtat ggatggtgag
     4741 cagatatacc gacttccaat cagaagaacc atagccaata gcctcgtgtt tggcatttta
     4801 tgcattggct tgagcgtggg cagtgctctc atcaccagtt caatgcaaaa tgaagggttt
     4861 cgtattctgg gatatatcct ggcaatcctt tttgcaatac ctgccatttt tttcattttc
     4921 caaatcatcc gcattgctcg cagcagatac gcaatcaatc gagatggctt gaccatttat
     4981 tgggggttcc aaaaaatggt cattccgatt caggaaatcg aatggattcg tccctatgat
     5041 caaatgggat atgccgtccc ctttccagct ctgggaaaga tcggcatgtt gtccggacat
     5101 atcttctacc aggacctggg agatatcctg ttctttgcaa caagccatga gaacgccttt
     5161 ttgatcggaa ccagccgaga ggtcttgttt ctatctccca tggatgcaca gcaattccaa
     5221 aaaggaatcc aggaagccgt ctattcaggt tctataaccc cattggagcg gaaatccatc
     5281 caaatggaat caccagtcaa tactgtgcgc gccaaccccg ccttgtacat tccccttgta
     5341 gtgggcattt tcctggcgct actgctgttt gtgctcttcg gattcatcgt caacacgcac
     5401 gataccatgc aggttggact catttttttt gaatctacag caagcgttgt catcatcccc
     5461 atactggctg tgatcatcaa tgggctgaac gcatttttcg cacccagaat ggcaaagaat
     5521 gataccttga aaccatatgg gattttgctg gctttcaccg gacttcttac cactctcttt
     5581 ctgatcatta ctttggtatt aggtctgttc tcttagaatt catctcatcc ctgtcaaaat
     5641 aatcagggag gagtccgtgc agtctgtttt cgaatgggca cttgaaggcg atgccgaagc
     5701 cccacgtcgt gcctgtagtg actttgttga gagaaattat ggacagttcg gagaaatggg
     5761 gaattccgtc agaaacgtga gctctggctg gtcaattttc tatgtcggca cgctttgaaa
     5821 ccactggaat tggaatggaa atctcaaacc atcagtcggt gaaccgatac catactagaa
     5881 aatacagtat taaagtacct agcccgcaat atccttttct cctacaatcg tacaagattt
     5941 gcgctttcaa ttacaataaa tcataatcaa aaacgaaaca gggatttacc atgaaaagtg
     6001 atgataagct ggaaaagctg aaagcaatgc gaaatgaagc tttcgtgggt ggtggagaag
     6061 acaggattgc caaacagcac gaaaaaggga aaatgaccgc acgcgaacgc ctggacaggc
     6121 tgttggacag tgggagtttt cacgagatcg gtcaattcat caccccacgc aacgattcca
     6181 ctgcccaagg ttcagataag accctgggtg atggcgttgt cacgggtt
//
LOCUS       DAOSDS010000117         6222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4527_length_6222_cov_5.499919, whole genome shotgun sequence.
ACCESSION   DAOSDS010000117 DAOSDS010000000
VERSION     DAOSDS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4527_length_6222_cov_5.499919"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="PLL88_10285"
     CDS             <1..301
                     /locus_tag="PLL88_10285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ01993.1"
                     /translation="TGSDFATLANRYDALTGGELGWFPRGYLYLKAIEDAAFALNAGQ
                     YSAVIESEYGYHIVQVMERSENQPLSTEALHVLQEQAVQDWIDAQWASSTIEVLV"
     gene            complement(306..1523)
                     /locus_tag="PLL88_10290"
     CDS             complement(306..1523)
                     /locus_tag="PLL88_10290"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HOJ01994.1"
                     /translation="MRIRSITYFFDPAHDSGATVFDTFRDHSNNLRRNLAAAGYQVQS
                     VRLATTPFSLWLDLGDESKALQQVSELCRRAHSADFDYISMGPCGVNDRAATSFIPAM
                     LALSPMLFTGMHLVDAQGRISLADITDCAGLIKQITTIEANGFANLRFAALANVKPFT
                     PFFPAAYGAAGERAFSFAMECADVAQETFKQAEDINAGCHTVCAIFTEHARKMEDLIE
                     KTSSSVPIIFKGFDFSLAPYPEDWCSFGKALEALGVAQIGSAGSVAAAAILAGMLEAG
                     TWKKSGFNGLMMPILEDSILAERSGAGLLSIYDVLQYSSLCGTGLDTVPLSGDIEEEK
                     IAALLLDVAALAVRLNKPLTARLMPIPGKKAGDAIEFDFAFFAKGKVLDYPYSGIQKP
                     LFAGETIQIQPRR"
     gene            complement(1520..2269)
                     /locus_tag="PLL88_10295"
     CDS             complement(1520..2269)
                     /locus_tag="PLL88_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOJ01995.1"
                     /translation="MQNPKIQWVRSLLAEKKRRQQEGAFVCEGIRLAEEAVRYSQKPL
                     LILFSSSISERGKNVLASYSCEKTEVAPHLLERISATETSQGLLLVMPFPRIPLPTKP
                     DLYLVLDEIQDPGNFGTILRAGTAFGLQGIFYTAGSVDPFSPKVVRAAMGAHFRIPVQ
                     EADPAKVKDISQSTQLFLTDVQHGTPCWKADLTQPLTIVIGSEALGISEAMRQIPHYS
                     VRIPMPGDMESLNAAVSASILLYEITRQRAT"
     gene            complement(2290..2859)
                     /locus_tag="PLL88_10300"
     CDS             complement(2290..2859)
                     /locus_tag="PLL88_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOJ01996.1"
                     /translation="MALSSQVYFETVFHDSNQLQLVQGDLTCEHVDAIVNAANRHLQH
                     GGGVAEAIARSGGRTIQQESDAWIREHGPLTHSRPAYTNAGNLACRYVIHAVGPMWGE
                     GDEEFKLHETIRGCFILAEELAITSLAFPAISTGIYGFPRRLAAAVFKEELFRYFEKN
                     PLTGLRLIRMVLWDTETLLTFLDEFKRTD"
     gene            complement(2946..3299)
                     /gene="rplT"
                     /locus_tag="PLL88_10305"
     CDS             complement(2946..3299)
                     /gene="rplT"
                     /locus_tag="PLL88_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOJ01997.1"
                     /translation="MTRVKSGPYRTRHHKKVLKITKGQFGSRSRLYRRANEAMLKSLW
                     YATRDRRVRKRDLRKLWITRINAAARLNGLSYSRLINAMKKADITLNRKMLADLAVRD
                     PQAFAAVVEKAKTAA"
     gene            complement(3379..3582)
                     /locus_tag="PLL88_10310"
     CDS             complement(3379..3582)
                     /locus_tag="PLL88_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HOJ01998.1"
                     /translation="MKTHKATAKRFRATGSGKLLRTKGGKSHLRRNTSERTKAQFTEM
                     IPVEGKGYQKRVKRLAPYIEKNH"
     gene            complement(3723..4244)
                     /gene="infC"
                     /locus_tag="PLL88_10315"
     CDS             complement(3723..4244)
                     /gene="infC"
                     /locus_tag="PLL88_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOJ01999.1"
                     /translation="MATSEVRANEEIRSATVRLIDQNGENIGIVPREKALQIARQANL
                     DLVEVAPNAEPPVCKVLDLGKYLYEQNKKERQARKAQTKVEIKEIRLRPKTNEHHRGF
                     KVEDARGWLEHGMKVLVTIRFRGREVTYPEIALEDLKEIAKELMDVAVIEKAPAMEGR
                     SMNMLLAPQKQPK"
     gene            complement(4360..6147)
                     /gene="thrS"
                     /locus_tag="PLL88_10320"
     CDS             complement(4360..6147)
                     /gene="thrS"
                     /locus_tag="PLL88_10320"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOJ02000.1"
                     /translation="MEKESKERYEDSHLYKVRHSTAHIMASAVLERFPNAKIAIGPAI
                     DRGFYYDFDLPRPLTPEDLNEIEARMRKLIKEHVKFEKKVITAEEGKQIFHDQPYKLE
                     LIEDLEKGQFDEHGNPLNEKPELSIYTSGNFTDLCRGPHVADSSEINPSAVKLLNVAG
                     AYWRGDEKRAMLQRIYGTAWEKSEDLQAYLSLLEEAKRRDHRKLGKDLELFSSVEEVG
                     AGLILWHPNGAKIRTIIEDYWRNEHEKADYDFVYTPHIGKAQLWQTSEHLGFYKENMY
                     SPIDIEGQEYYLKPMNCPFHLHIYKSKIRSYKELPIRYAETGTVYRYERSGVLHGLLR
                     VRGFTQDDAHHFCSPEQMPQEIDFVLNFCLDMLRAFGFTEFTAYLSTRPEKAVGDPQR
                     WKEAEAALEASLKRAGIAYDVDEGGGAFYGPKIDLKIKDALQREWQLSTIQFDFNEPE
                     KFDLTYIGEDGEQHRPYMVHRALLGSLERFFGVLIEHYAGAFPVWLMPVQAMIIPIAD
                     RHLAYAEELKKTIRAAGLRVKVDDRSERMNAKIRDAQNEKIPYMLIVGDQEMEKQQVS
                     LRLRNGENPGVTDVAAFLDRARKDIDAKV"
BASE COUNT         1353 a         1632 c         1625 g         1612 t
ORIGIN      
        1 aacggggagc gattttgcta ctctggcgaa tcggtacgat gcgctcacgg gcggtgagct
       61 gggctggttc ccgcgcggtt acctctatct caaagcgatt gaggatgcag ccttcgcttt
      121 aaatgccgga caatacagcg cagttatcga gagtgaatac ggatatcaca tcgttcaggt
      181 catggaacgc agtgaaaacc aaccgctaag caccgaggcg ttgcatgttc tgcaagagca
      241 ggcagtgcaa gattggattg atgcgcagtg ggcgtcttct acgattgaag tgttggtgta
      301 aattgctacc tgcgtggctg gatctggata gtttccccgg cgaagagtgg cttttgaata
      361 cctgagtaag gataatcaag cactttccct ttcgcaaaga aagcgaaatc gaattcaatc
      421 gcgtcccctg ctttttttcc gggtatgggc atcaagcgcg ccgtaagcgg cttgttcaat
      481 cgcacagcaa gcgctgcaac atccagcaga agcgcggcaa tcttttcctc ctcgatatcg
      541 ccggataatg ggacggtatc gagccctgtc ccacaaaggc tggaatactg cagcacatca
      601 tagatcgaaa ggagccctgc tccgctacgt tcagccaaaa tgctgtcttc cagaatcggc
      661 atcatcaacc cgttgaaccc gctcttcttc caagtgcccg cttctagcat tccagctaaa
      721 atggcggctg ccgcaacgct ccctgcagag ccgatctggg ctacccccaa cgcttccaga
      781 gctttgccga atgagcacca gtcttcgggg tatggcgcta gcgagaaatc gaaacctttg
      841 aaaatgatcg gaacgctgga agatgttttt tcgatcaggt cttccatttt tctggcatgc
      901 tctgtaaaga tcgcgcatac tgtatggcag cccgcattga tgtcttccgc ctgtttgaag
      961 gtttcttgcg ccacatcggc acattccatg gcaaaagaga aggcgcgttc gcccgcagct
     1021 ccgtaggcgg ctgggaaaaa gggggtaaaa ggtttgacgt ttgccagcgc ggcgaaacgt
     1081 aggttggcga acccatttgc ttcaattgtg gtgatctgtt tgatcaaacc ggcgcagtcc
     1141 gtgatatctg ccagggatat tctgccctgc gcatccacca gatgcatgcc ggtgaagagc
     1201 atgggtgata aagccagcat agcgggaata aaagatgttg ccgcgcgatc attcaccccg
     1261 cagggaccca tagaaatgta atcaaaatca gcgctgtgcg cccgacggca cagctcgctc
     1321 acttgttgca gagctttgct ttcatccccc aggtcaagcc aaagcgagaa aggggtggtt
     1381 gccaagcgca cgctctggac ctggtacccg gctgctgcca gattccttct taaattgtta
     1441 ctgtgatcgc gaaatgtatc gaagacggtc gcaccgctgt catgcgcggg gtcgaaaaaa
     1501 taggtgatgg aacgtattct catgttgccc tctgccgcgt gatttcatac agcaaaatgc
     1561 tggctgaaac agccgcgttc aaactctcca tatctcctgg catgggaatg cgcacgctgt
     1621 agtggggaat ctgacgcatt gcttcggata tgccaagtgc ttcgcttccg atcacgatcg
     1681 tcagcggttg ggtaagatca gctttccaac agggggtgcc atgctgcaca tcagtcagga
     1741 agagctgagt gctttgggag atgtctttca cctttgcagg gtccgcttcc tggacaggga
     1801 tgcgaaaatg ggctcccatt gcagcacgca ccactttggg cgaaaatgga tcaacgctgc
     1861 ccgcagtgta gaagatgccc tgcaacccga aggctgttcc tgcccgcaga atggtcccga
     1921 agttgccggg atcttggatt tcatcgagaa ccagataaag gtcaggtttg gtcggcagcg
     1981 ggatgcgcgg gaatggcata accagcaaca atccctggga ggtttctgtg gcagaaatgc
     2041 gctcgagcag gtggggggcg acctctgttt tctcacagga gtatgaagca aggacgtttt
     2101 ttccgcgttc cgagatggag gaactgaata gaatcaggag tggtttttgc gaataacgca
     2161 ctgcttcttc ggcaaggcgt atcccttcac aaacgaacgc tccttcttgc tggcgccgtt
     2221 ttttctccgc cagcagcgag cgcacccact ggatcttcgg gttttgcata gaagtcagca
     2281 tagtttgggc taatctgtcc ttttgaactc gtccaaaaaa gttaacagcg tttccgtgtc
     2341 ccacaagacc atacggatca gacgcaggcc tgtcagtgga ttcttttcaa aataccggaa
     2401 gagctcttct ttgaacaccg cagccgctaa tcggcgcggg aatccgtaga tgccggtgga
     2461 gatggctgga aatgccagag aggtaatggc gagttcctct gccaaaataa agcaacccct
     2521 gatcgtttcg tgaagtttga attcttcatc tccttcaccc cacatggggc cgactgcgtg
     2581 gatgacgtag cggcaggcta gattgccggc gtttgtgtat gccggtcgcg aatgggtgag
     2641 cggtccgtgt tctctgatcc aggcatcgct ctcctgctgg atcgttctgc cgccactgcg
     2701 cgcaatggct tccgccacgc ctccaccgtg ctgaaggtgg cggttggctg cgttcacaat
     2761 ggcatccacg tgctcgcacg tcagatcacc ctgcacgagc tgcagttggt tgctatcatg
     2821 aaataccgtt tcgaaatata cttgtgaaga taaagccatg tgtttatttt aatggattaa
     2881 ttgaaaacaa agacggagtg gaaaccttcc actccgtcat cttatttaaa ggagaatttt
     2941 ctttcttaag ctgctgtttt tgctttttca acaacagccg cgaacgcttg cggatcgcgg
     3001 accgccagat cagccagcat cttccggttg agggtgatat cggctttctt catggcgttg
     3061 atcaatcggc tgtagctcaa tccgttcaag cgagcagcgg cattgatgcg agtgatccat
     3121 agctttcgca aatcgcgttt gcgcactcgc cgatcgcgcg ttgcgtacca taaacttttc
     3181 agcatggctt cgttggcgcg cctgtacagg cgcgaacggc tgccaaactg acctttggtg
     3241 atctttaaaa cttttttgtg atgcctggtc cggtacggac cactttttac gcgagtcatt
     3301 ttctctcctt tgcaaacccc tgggcgtcgg ttccaaacca gttgcgtaca cccagcagcc
     3361 gcaaataatc attccttgtt aatgattctt ttcaatatag ggcgcaagtc tcttcacgcg
     3421 tttctgataa cccttccctt ccaccggaat catctcggtg aactgcgctt tggtgcgctc
     3481 agaagtgttg cggcgaagat ggctttttcc gcctttggtg cgcaaaagct tgccagaacc
     3541 ggtagcccgg aagcgtttgg cagtcgcttt gtgcgtcttc atcttatatt ttgtggtttt
     3601 agcttttcgc ggcacagcag tactccttaa ataattaaat agaaagtcct ttaaataagg
     3661 gcaagcgtcc attatactgg aaaaaccctg atttgccaat gaaggcgtga aaagattttc
     3721 ggttacttcg gttgtttctg tggtgcaagc agcatgttca tggaacgacc ttccatggca
     3781 ggcgcttttt cgatcacggc aacatccatc aattctttgg cgatctcttt cagatcttcc
     3841 agagcgatct cgggataggt cacttcacgc ccgcgaaagc ggatggtgac cagcactttc
     3901 ataccgtgtt ccaaccaacc gcgcgcgtcc tccaccttaa aaccgcgatg gtgttcattg
     3961 gttttagggc gcaggcggat ctctttgatc tccaccttgg tctgtgcctt gcgtgcctgg
     4021 cgttcctttt tattttgctc gtacagatac tttcccagat caagcacctt gcagaccggc
     4081 ggctccgcgt ttggagcgac ttccaccaga tcaaggttgg cttggcgtgc tatttgcagc
     4141 gccttttctc tgggcacgat tccaatattt tccccgtttt ggtcgatcag gcgaacggtt
     4201 gctgatcgaa tttcttcatt tgctctaacc tctgaggtag ctataagaat atctcctttc
     4261 ctttttcaat ggtttgatat taccataaac tgaaaataca agtcaacatc caaaaatcat
     4321 agagaaacag cattcatttt taacacgcag tcactcgggt tagactttgg cgtcaatgtc
     4381 cttgcgcgcc cgatccagaa aagctgccac atccgtgacg ccagggtttt cgccattgcg
     4441 caatcgcaac gaaacctgtt gtttctccat ctcctgatca cccacgatca gcatgtaggg
     4501 tatcttctca ttctgtgcat cgcggatctt ggcattcatg cgttcactgc ggtcatccac
     4561 tttcacgcgc aatccggcgg cgcggatggt cttcttcaat tcctccgcgt atgccaggtg
     4621 gcggtctgcg atggggatga tcatggcttg cacgggcatc aaccacaccg gaaatgctcc
     4681 ggcataatgc tcgatcagca cgccaaagaa gcgttccaga ctgccgagca gggcgcggtg
     4741 caccatgtag ggtcggtgtt gttcgccatc ttcgccaatg taggtgaggt cgaatttctc
     4801 gggctcgttg aaatcgaatt ggatggtaga gagctgccat tcgcgctgaa gcgcgtcctt
     4861 gatctttaga tcgatctttg gaccatagaa tgcgccgccg ccctcatcca catcgtaagc
     4921 gatgccagcc cgcttgaggg atgcctccag cgcggcttcc gcttctttcc agcgctgtgg
     4981 atcgcccacg gctttttccg gtcgcgtgct gaggtatgcg gtgaactcgg taaaaccaaa
     5041 ggcgcgcaac atatcaaggc agaagtttaa tacgaaatca atctcctgcg gcatttgctc
     5101 gggcgaacag aagtggtggg cgtcatcttg ggtgaagccg cgcacgcgca agagtccatg
     5161 cagcacgccg ctgcgttcgt accggtagac cgtgccggtt tcggcataac ggatgggcag
     5221 ctctttgtat gaacgaatct tacttttata aatatgcaga tggaatggac agttcatggg
     5281 tttgaggtag tattcctgac cttcgatgtc gatgggggaa tacatgttct ctttgtagaa
     5341 ccccagatgc tccgaggtct gccacaactg cgcttttcca atatgcgggg tgtacacaaa
     5401 gtcgtaatca gccttctcgt gctcattgcg ccagtaatct tcgataatcg tgcggatctt
     5461 ggctccattg ggatgccata gtatcaaacc agcgcccacc tcttccacag aactgaacag
     5521 ttccaggtct ttgccgagct tgcgatgatc gcgccttttg gcttcttcca gaaggctcag
     5581 atacgcttgc aggtcttctg atttttccca ggctgtgccg tagatgcgct gcagcatggc
     5641 gcgcttttca tccccgcgcc agtacgcgcc ggcgacgttg agcaatttga ccgccgacgg
     5701 attgatctct gaagaatctg ccacatgcgg tccacggcac aggtcggtga aattcccgct
     5761 ggtgtagatg gaaagctcgg gcttttcatt gagcgggttg ccatgctcgt cgaactgccc
     5821 tttttcaagg tcttcgatca actccagctt atatggctgg tcgtggaaga tttgcttgcc
     5881 ctcttctgcc gtgatgacct tcttttcgaa cttgacatgc tccttgatca acttgcgcat
     5941 gcgcgcttcg atctcgttca gatcttccgg ggtgagcggg cggggcaggt cgaaatcgta
     6001 gtaaaaaccc ctatcgatag ccggaccgat ggcgatcttg gcgttgggga atctctccag
     6061 cacggcggat gccatgatgt gagcggttga atggcgcacc ttatacaggt gtgaatcttc
     6121 gtaacgttct ttactctctt tttccatgct aacctccagt ttgcaaatta aacaaaaacc
     6181 cgctccattg ctggggcggg agtgttctca cgcggttcca cc
//
LOCUS       DAOSDS010000118         6190 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4559_length_6190_cov_4.343113, whole genome shotgun sequence.
ACCESSION   DAOSDS010000118 DAOSDS010000000
VERSION     DAOSDS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4559_length_6190_cov_4.343113"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLL88_10325"
     CDS             <1..946
                     /locus_tag="PLL88_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOJ02001.1"
                     /translation="NLKFIYEGEEEIGSPSIVEFLRDHTELLKSDFVLNLDAGMLGAN
                     APTIVYGLRGLAYFEIHVQGPDHDLHSGLFGGVVYNPVQALAELIAGMKDKDGKILLP
                     GFYDSVKPLSEHERKELARLNIGDDFYIEQTGVQKLFGEIGYTAVEQVGARPTLDVNG
                     FKAGYIGEGPKTVIPSTAMAKISMRLVPDQTPQMVNEQLLAYMREHAPEQITWKVVQL
                     SSDPASIADPDHFATQTFAKALKEVWGVDPIYKREGGSIPIVSHMKNFLGTESVLSGF
                     CLPGDLIHSPNEHLHLPTWKKGILATIHFLYELGEQNA"
     gene            939..1820
                     /locus_tag="PLL88_10330"
     CDS             939..1820
                     /locus_tag="PLL88_10330"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOJ02002.1"
                     /translation="MPEKKSAPLESYGGQALIEGVLMRGRKFLTAAFRLPDGSIKVVE
                     EKLSGIYTKKIRDIPFLRGLIILWDSLVLGMKYLTLSSNMQAKEDEKIEGPALYLTLI
                     VSLLIGLTIFFFLPTLLLQLLYKTSLSVFFINLIEGTLRLLILIGYLLMIRKMEEIKR
                     VFAYHGAEHKTISAYENGDMGDVRAIQKYPTAHARCGTSFLMSVMVVSILVFSVFSTP
                     NILLRLLSRVILIPVIAMLSYEFIRILGKYDENPMVRILAKPNLWLQKLTTNQPDDAM
                     VEVAVQAFNRLLELEQS"
     gene            1832..2278
                     /locus_tag="PLL88_10335"
     CDS             1832..2278
                     /locus_tag="PLL88_10335"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOJ02003.1"
                     /translation="MSIVEKIEQDLFNAMKEKNAEIVRTLRMAMSSIKYAEKEKRESL
                     DDLGVISVLQKEVKIRQETMEDAKKGNRQDIIAENEHDIKVLEKYLPQPLTEAELDAL
                     VQKIIEETGAESMKDMGKVMPVAVQQVNGRAPNNVISQAIRKFLAG"
     gene            2282..4333
                     /locus_tag="PLL88_10340"
     CDS             2282..4333
                     /locus_tag="PLL88_10340"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOJ02004.1"
                     /translation="MGNLRPKFSLKTLLTFFVFAVLSYTSLTLPFTYRQSPVSLSIGS
                     VSTQDIRASQTFSFVSETLTEEARDRAEQSVSPIYLAADPTISRQQLENMRAILNYIS
                     SVRNDQFATPEQKISDLQAISNLTITSEMATNILAFSQEKWQEIQNEALFVLEEVMRS
                     TIREDQLTQAKRSVLPLISYSFSTVETEIINSLVMPMVVANSLFSSEKTNEAIQQARE
                     DVAPVTKTYMAGETIVSTGQVITPVMWEALQELGLISPQSTMLKYISSALLVFSVVVM
                     EYIYVFRYRKNLLGADFRSLVTILALYLIFLFLARIFIVNRAVVPYIFPIAAFGLTIS
                     SLIHYEIGIIFSIGLSTLTAYGQSNSVELTLFYVIASIVAIFVLQRGRRITAFFSTGL
                     VLGLIGSATVVAYRLISTYFDMEGILTLIGASFLNGMASVSLTLILQYAVASLLGKTT
                     ALQLMDLSRPDHPLLQLIMSNSPGTYQHSLQVANLAEQAARSIDADPLLTRVGALYHD
                     AGKALNPSFFIENQVTGNLDTHEDLDPAETAGIIIKHVEDGIRLAREYRLPPEIEAFI
                     TEHHGKTTTRYQLSKAKEQSGKDKELDLAKFEYPGPNPHTRETAILMMADKVEARARA
                     EIPKSDEDIKKLIETSIDSLLQEKYLDNTNLSMKNIQSIKESFFNTLKNTYHHRLKYP
                     K"
     gene            4381..4773
                     /gene="ybeY"
                     /locus_tag="PLL88_10345"
     CDS             4381..4773
                     /gene="ybeY"
                     /locus_tag="PLL88_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOJ02005.1"
                     /translation="MFRKNAQRALQLLDLENKDVSLKICDDDEMRALNRKYRSIDKTT
                     DVLSFSMDFKEPQSNNEYLGDIMISFQTANKQARAHHQSLETEMLFLFIHGLLHLIGY
                     DHETLDDERKMFSRQEEILFKITGKSNE"
     gene            4766..5134
                     /locus_tag="PLL88_10350"
     CDS             4766..5134
                     /locus_tag="PLL88_10350"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOJ02006.1"
                     /translation="MNNKYTVRESFQFAFEGFRYALQTQKNLRFQLVVAVVVITLSTF
                     LPMQLIEWALLLVLIALVVGTEILNTAIEKTIDLIVKEYDPLAKTIKDLSAAAVLFIA
                     ITAVVVGLFIYLPKFQMLFP"
     gene            complement(5167..5823)
                     /locus_tag="PLL88_10355"
     CDS             complement(5167..5823)
                     /locus_tag="PLL88_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ02007.1"
                     /translation="MPKSKIMIVDDHEVVRLGLISLLEQNSQYEVVAQADNALDAIKK
                     VETHNPDIVLMDIRLPGMSGIEACEQICKKYPNTKVIMLTSFAEDEMLFSAIKAGASG
                     YVLKQINARDLINSIESVARGEASLDPAVTQRVFQEVRKAVREEEASAFSSLSQQEKM
                     VLKLVSEGKTNRDIAQNLFLGEGTVRNYVSSILSKLNVSNRAEAAAYAVEHNLKDYLN
                     "
     gene            complement(5838..>6190)
                     /locus_tag="PLL88_10360"
     CDS             complement(5838..>6190)
                     /locus_tag="PLL88_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOJ02008.1"
                     /translation="NGQKIIITGDFNTAHKEIDLKNPKENEKNTGFLPEERVWIDRYL
                     EHNLVDIFRERYPDEVQYTWWTYRFKARERNIGWRLDYFLISAELIPMVLDVTIHDEV
                     LGSDHCPVTLELNY"
BASE COUNT         1678 a         1431 c         1381 g         1700 t
ORIGIN      
        1 taatttgaaa ttcatctatg aaggcgaaga agagatcggt tcaccttcca tcgttgaatt
       61 cttaagagac cataccgaac ttctcaaatc tgattttgtg ctgaacctgg atgcgggaat
      121 gcttggcgcg aatgccccaa ccatcgtata tgggctacgt ggtctggctt attttgagat
      181 acacgttcaa ggtcccgatc atgatctgca ttccggtctg tttggaggag ttgtctacaa
      241 ccccgtccag gcgttagccg aactgatcgc cggcatgaag gataaggacg gaaaaatcct
      301 gctgcctggt ttttatgatt ctgttaaacc gcttagcgaa catgaacgaa aagaactggc
      361 tcgtttgaac atcggtgacg atttttatat agaacagacc ggggtgcaga aactcttcgg
      421 cgaaatcggt tataccgccg tcgaacaagt tggcgcgcgc cccacgctgg atgtgaatgg
      481 ctttaaagcc ggatacattg gagaagggcc aaaaaccgtc atcccttcaa ctgcgatggc
      541 aaagatctct atgcgcctgg tccccgatca gacgccgcaa atggtcaacg agcaattgct
      601 tgcctatatg cgcgaacatg cgcccgaaca gattacctgg aaagtggtac aactatccag
      661 cgatccagcc agcattgcag atccggacca ttttgctacc caaacctttg ccaaagcatt
      721 aaaagaggtg tggggtgttg atccaattta caaacgcgaa gggggcagta ttcctatcgt
      781 gtcacatatg aaaaatttcc ttggtaccga atcggtattg agcgggttct gcctccctgg
      841 agatctgatc catagcccca atgagcacct gcacttgccc acctggaaaa aagggatcct
      901 ggctactatt catttcttgt atgagctggg agaacaaaat gcctgaaaaa aagtcagcgc
      961 cattggaaag ttacgggggt caggcactga tcgaaggggt cttgatgcgc ggaaggaagt
     1021 tcctgaccgc ggcatttcga ttgccagatg gttcgatcaa ggtggttgaa gaaaagttaa
     1081 gcggcatcta cacaaagaag attcgcgata tcccctttct gcgtggtttg atcatcctgt
     1141 gggattcatt ggttctggga atgaaatacc tgaccttatc ctcaaatatg caagcgaagg
     1201 aggatgaaaa aattgaagga cccgcattat atctcacgct gatcgtttca ttgctcattg
     1261 gattaaccat atttttcttc ttaccgacct tgctgttgca gcttctctat aaaaccagcc
     1321 tctccgtgtt tttcattaat ttaattgagg gcactttgcg actgctcatt ttgattggtt
     1381 atctgctgat gatcagaaaa atggaggaaa tcaaacgcgt tttcgcttat catggggctg
     1441 aacacaaaac cattagtgcc tatgaaaatg gcgatatggg ggatgtgcgt gcgatccaaa
     1501 aatatcccac tgcgcacgcc cgctgcggta cttcttttct catgtcggtg atggtggttt
     1561 cgatcctggt cttttccgta ttttcaacac ccaacatcct tttgcgcttg ttgagcaggg
     1621 tcattttaat tcccgtgatc gccatgctat cttacgagtt catccggatt ctaggaaagt
     1681 atgatgaaaa tccaatggtc cgcatcctgg caaagcccaa tctttggctt caaaaactta
     1741 ccaccaacca accggatgac gccatggttg aagtagcggt acaagctttt aaccgattgt
     1801 tggaattgga acaaagttaa ggagaatgag catgagcatt gttgaaaaga tcgagcaaga
     1861 tttgttcaat gcgatgaaag aaaaaaatgc tgaaatagtg cgcacattgc gcatggcaat
     1921 gtcttccatt aagtacgctg agaaagaaaa acgagaaagc ctggatgatc tgggcgttat
     1981 cagcgttttg caaaaagagg tcaagatccg ccaggagacc atggaagatg caaaaaaggg
     2041 aaaccggcaa gatattatcg ctgaaaacga acatgatatc aaggtgttgg aaaagtattt
     2101 gccgcaaccc cttactgaag ctgaactgga tgccctggtg cagaaaatca ttgaggagac
     2161 cggagcggag tccatgaaag atatgggtaa ggtcatgcct gttgctgtgc aacaggtgaa
     2221 tggtcgcgct cctaacaatg tgatcagcca ggctattcgc aaatttcttg caggatagac
     2281 tatgggaaat ttaaggccga agtttagcct caaaacgctt ctgactttct tcgtttttgc
     2341 agtgctcagc tacacttctt tgactctccc ctttacctac aggcaatcgc ctgtttcttt
     2401 aagtatcggt agtgtatcta cacaagatat tcgcgcttca cagacatttt cgttcgtcag
     2461 tgaaacgctg acggaagaag cgcgcgatag ggctgaacaa tcggtctcgc cgatctatct
     2521 cgcggcagac cctaccatca gcaggcagca acttgagaat atgagagcaa ttctcaacta
     2581 catcagttct gtgcgcaacg atcaatttgc gacgccggaa cagaagatct ctgacctgca
     2641 agctatcagt aatcttacta ttaccagtga aatggcaacg aacattctgg ctttcagcca
     2701 ggaaaagtgg caggaaattc aaaatgaggc tttgttcgtt ctcgaagagg tgatgcgcag
     2761 cacaatccgt gaggatcaac tgacgcaagc caaacgcagc gttttgccct tgatcagtta
     2821 ttctttttcc acggtagaaa cagagatcat aaactctttg gtcatgccga tggtggttgc
     2881 caacagcctc ttcagcagcg aaaagaccaa cgaagctatt caacaagcca gagaagatgt
     2941 tgcccctgta accaagacat atatggctgg cgagaccatc gtttccaccg gtcaggtgat
     3001 cacacctgtc atgtgggaag cactacagga attgggtctc atctctcccc aaagcacgat
     3061 gttgaagtat atttcctccg ccctgctggt gttttccgtg gttgtgatgg aatatattta
     3121 tgtttttcga tatagaaaga acttactggg agctgatttc aggtcactgg tgaccattct
     3181 ggcgttgtat cttatttttc ttttccttgc gcgcatcttc attgtgaacc gtgcggtagt
     3241 tccttacata ttccccatcg cagcgtttgg gttaactatt tcaagtctta tccattatga
     3301 aatcggcatc atcttctcga taggattgag cacgttaact gcatatgggc agtcaaattc
     3361 ggtagagtta actcttttct atgttattgc aagcatagtg gcaatctttg ttctccaacg
     3421 tggacgcagg atcacggctt ttttctctac cggattggtg ctggggttga ttggttcagc
     3481 cacagttgtg gcttaccgat taatctcaac ctattttgat atggaaggca tcctcactct
     3541 tatcggagca tcattcttga acggcatggc atcggtcagc ttaactttga tattacagta
     3601 cgctgtcgcg tcattgctgg gcaaaactac cgccctgcaa ctgatggatc tctccagacc
     3661 cgatcatccc ttattgcagc tcatcatgtc caattcccca ggaacctatc agcatagtct
     3721 tcaggtagcc aatctcgccg agcaggcagc gcgtagcatt gatgcagacc ctttgctcac
     3781 tcgcgtcggc gccctttatc atgacgctgg aaaagcttta aatccatcct tctttatcga
     3841 aaaccaggtg accgggaatc tggataccca cgaagatctc gatccggctg agaccgctgg
     3901 catcatcata aagcatgtag aagatggtat cagactggct cgcgaatatc gcctgccacc
     3961 tgaaatcgaa gcctttatta cagaacatca tggaaaaacc accaccaggt atcagcttag
     4021 caaagccaaa gagcaatcag gaaaagacaa agaacttgat cttgcaaaat tcgaatatcc
     4081 aggacctaac ccccatacca gggagactgc catcttgatg atggctgata aagttgaggc
     4141 gcgcgcaaga gcagaaattc ccaaatcgga tgaagatatc aagaaattga ttgaaacatc
     4201 tattgattcg ctgctgcaag aaaaatattt ggacaacaca aatttatcca tgaagaatat
     4261 tcagagcatc aaagaatcct ttttcaatac tttaaagaac acatatcatc accgcttgaa
     4321 gtaccccaaa tgatagatct gatcttccca ttccaccttc gattctggat gaatctgaaa
     4381 atgttccgga aaaatgcgca gcgcgcattg caacttttgg atctggaaaa caaagatgtg
     4441 tcgctgaaaa tctgtgatga cgatgaaatg cgcgcgctga atagaaagta tcgcagcatt
     4501 gataaaacga cagatgttct ttctttttcc atggacttca aagaacccca gtcgaataac
     4561 gaatacctgg gagacatcat gatttcattt caaaccgcaa acaagcaagc ccgagcccat
     4621 caccaatctc ttgaaactga gatgctcttc ttgttcattc atggtttgct gcatttgatc
     4681 ggttatgatc atgagacgct tgatgatgaa aggaagatgt tctcacggca ggaagaaatt
     4741 cttttcaaga tcacgggaaa atccaatgaa taataaatac accgttcgcg aatcttttca
     4801 attcgccttt gaaggctttc gctatgccct ccaaacccag aagaatctgc gctttcaact
     4861 ggttgtggca gttgttgtga tcacactgag cacctttcta ccgatgcagc ttattgagtg
     4921 ggcgttgttg ctcgtgttga tcgcattggt cgttggaacg gagattctca atacggctat
     4981 tgaaaaaacc atcgatctta tcgtaaagga atatgacccc cttgcgaaga caatcaagga
     5041 tttaagtgca gcagcagtcc tgtttattgc aattacagca gtggtggtcg gtctgtttat
     5101 ctatttacct aagtttcaaa tgctctttcc atgaagagcg acggggtttc catcgttctt
     5161 ttatttctaa ttcagataat ctttgaggtt gtgctccact gcatacgctg cagcttctgc
     5221 acgattgctt acgttgagtt ttgagaggat gctacttacg taattgcgca ccgtgccttc
     5281 ccccaagaac aggttttgcg caatatcgcg attggttttg ccttcagaaa ccaatttcag
     5341 caccattttt tcttgttggc ttaaacttga gaatgcagat gcttcttctt ctcgaaccgc
     5401 ttttctcact tcttgaaata cccgttgtgt cacagccgga tcgagggatg cctcaccgcg
     5461 tgcaacggac tcgatcgaat tgatcaggtc ccttgcattg atctgtttga gaacgtatcc
     5521 tgaagctccc gccttgatag cagaaaaaag catttcatct tcggcaaatg acgtaagcat
     5581 gataactttc gtattcgggt attttttaca aatctgctca cacgcttcaa tgcctgacat
     5641 gcccggcaga cggatgtcca tcaaaacgat gtcggggtta tgggtttcaa ccttctttat
     5701 ggcatccagg gcgttatcgg cttgagcaac cacttcatat tgcgagtttt gctccagcaa
     5761 tgaaatcaat cccaatctga cgacttcatg gtcgtccaca atcatgatct tgcttttcgg
     5821 cattcaacct ccatatttca atagtttagt tccagagtta ctggacaatg atcagatcct
     5881 aagacctcat catgtatcgt cacatcaagc accatgggaa tcagttcagc agagatcagg
     5941 aaataatcca gacgccagcc aatattcctc tctcgagctt taaagcggta ggtccaccat
     6001 gtatattgca cttcatcagg ataacgctcc ctgaagatat cgaccaggtt gtgttccaaa
     6061 tagcggtcta tccacacacg ctcttcgggc aaaaaacccg tgttcttttc attttctttc
     6121 gggttcttca aatcgatttc cttgtgagcc gtattgaaat caccggtaat gataattttt
     6181 tgcccattcg
//
LOCUS       DAOSDS010000119         6132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4618_length_6132_cov_4.467007, whole genome shotgun sequence.
ACCESSION   DAOSDS010000119 DAOSDS010000000
VERSION     DAOSDS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4618_length_6132_cov_4.467007"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLL88_10365"
     CDS             <1..548
                     /locus_tag="PLL88_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOJ02009.1"
                     /translation="RKMQKPTRHPCVSVFLFLIITSGCVWLERSSGIPYASLESTVTA
                     LSEESRGLLDVSLPACVRASDAFEIAYVTRVIDGDSIAVELNGKSEQVRYVGINAPEY
                     DGGEWKEAEQATQLNRDLVEGRYVLLIKDVSERDKYGRLLRYVFTGTHFVNYELVRQG
                     VAEAREYPPDTACQPYFQQAE"
     gene            complement(764..1561)
                     /locus_tag="PLL88_10370"
     CDS             complement(764..1561)
                     /locus_tag="PLL88_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02010.1"
                     /translation="MKRKLNTASLCMLIILLSFSACQGMKTTASDQNSFGSQNGVESE
                     EPQVYTEPDDIKIMLDELERLRWEQWLAQPGWWHEQKVIQSQSGNLHGTSAEWWFQHS
                     DAATCPMILQIIYQEDGQVLETSVLISESKIPTVSQNVGASGAENGVLLVKIPDQSCP
                     QLLESSLEHIKQMLNDSTTLKSAQAVIQDGALTITLSQEEEPYRQEITVTIDTASGFI
                     TYEKVQLYVNNRENPEGEIEYNYLHQHYDELPSEIQNQLDQGLAKLQ"
     gene            1688..3838
                     /locus_tag="PLL88_10375"
     CDS             1688..3838
                     /locus_tag="PLL88_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HOJ02011.1"
                     /translation="MNKNILKTAIQLSVVGLMLISAMHMNIGKVEAQAESADETPQVI
                     SPQNVQDLKLLRWIGEGAYTGTIAQQTDGNLIAAATTAGVKLLDRESGEQIGFIPIGL
                     EPTALSISPDGNTLAVVVNYPDGSLGGFMGLPGFNPQIQFYSLPAGEKQGEAIDELGE
                     CGESNILAIAYSPEGSELVFERKHSAQNDGRRFCVLSITERKIVRTKDVSDSAQMVIS
                     PQGDFVAVITANSQAESSTVSIYSTEDFHLATEFEINQTVFSYNFSFSQDGQYLGLNS
                     YEEDQNSTVYSFQVWDLEDGELVFSEEPTDDMVQSFDVDSNRSALIVGTQFGYVKIYS
                     LNTGKMEKQLGPFTWTNYSQTGNPGGVTAAETPASIKSVIVSRSGESLTVSDYMTTYG
                     QSSHIRIFDMPDGGAGADFVSTSMGSESLQIAFSPDSSQIALAGSLDGRVDVFNAQNG
                     ELALTLSGHTQVVNQVIFSPDGNVIATGSNDNTVRLWNAQTGALLRVLEGHQGRVNRL
                     VFSPDSTWLLSGADDNSLRRWNVSDGELLETLELGNENWRVEFLDILNDNVSVVYRII
                     KYPSPYIGFITKQMIWNTQSGESQNIGGSDISISGLSADKDLFMGFSSGKMVGTLQED
                     GSMKTITTFRSPYGNGALTKPAISPDNQLVIAGNGFGLHAWELNGSSLNFLGLIAVSE
                     PVPSYGDEYLFSPDRKYLAYTAGGVAYLMGVIEK"
     gene            3974..4360
                     /locus_tag="PLL88_10380"
     CDS             3974..4360
                     /locus_tag="PLL88_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ02012.1"
                     /translation="MDYIFDDNQPIYLQIKQRICADILRRKYSPGDKLPGIVETAMQF
                     KVNHNTIQRVYQELIREGVAISKRGEGTFVTEDTKVLKKMETELQTYAMEGFIEEMVR
                     LGYQEGDLAQIIEQYIHSRQVKRKEE"
     gene            4367..5155
                     /locus_tag="PLL88_10385"
     CDS             4367..5155
                     /locus_tag="PLL88_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02013.1"
                     /translation="MPNIYREELSRALVNKRFLLVFLLAGISFLYGFFQMKTIVVEPL
                     DAITIWQGILQRGYYGFFACVMAVLPFADSLSVDKNERVINHFLLRVEYKKYIAAKFL
                     SAIVSGAVAVLLPAILMLLICALMYPANPVQIPTLSFSVKEILPGQVISPGTALKLTP
                     LGYLALCWLFLAFFGMAYAMLAMGLSFHTENSLIVLGFPFLCYSFGYYFIPSSRHLHW
                     LISSEAALIPQGNLISAVVQWVGFGLFFCANVLLFGNKERQVIK"
     gene            5152..6003
                     /locus_tag="PLL88_10390"
     CDS             5152..6003
                     /locus_tag="PLL88_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02014.1"
                     /translation="MTAMKSRAFLKYQSAHYFLRWRWLLPIPVGLCLGYWAQKVISVL
                     TPPFSSGLQGNALEAFIWAFGKPEIIYFVISILFIFLVSDLLPENSYGQQVLLRLRSR
                     KTWWLGKTIFVFLSTLVYAFLLIGSFFLPVLMKYPISTEWSAAGVSTYGIGLGYSLKN
                     GSPLQAFGSILSFLLIGWFAIGLFIQIVNLISQHRWVGFFSGVLIVICASLGDIKGGP
                     IGGEGIGSFFLLQNHLEFTPLWAPVRVIPEIYSWLFWLIWILACLGSGFMLCKKIDFF
                     LVNHQED"
BASE COUNT         1457 a         1340 c         1585 g         1750 t
ORIGIN      
        1 ggagaaaaat gcaaaaaccg acccggcacc catgcgttag tgtattcctt ttcttgataa
       61 tcaccagcgg gtgcgtctgg ttagagcgat catctggtat tccctatgcg tcattagaaa
      121 gcacggtaac cgcgctctct gaggaatccc gtggactgct ggatgtttcc ctgcctgcct
      181 gcgtgcgagc atccgatgcg tttgagattg cctacgtcac ccgcgtgatt gacggggatt
      241 ccattgcagt ggaactgaat ggcaagagcg agcaggtgcg atatgttggc atcaatgctc
      301 cggaatacga tggcggcgaa tggaaagaag ccgaacaagc cacgcaattg aaccgtgacc
      361 tggtggaagg gcgttacgtg ttgcttatca aggatgtttc tgagcgcgat aaatacggtc
      421 gattgctgcg ctatgttttc accggaacgc atttcgtgaa ttatgaactg gtgcgtcaag
      481 gtgtggcgga agcgcgggag tacccaccag acactgcttg ccaaccgtat tttcaacagg
      541 cagaataatt catcggtgag cacccataaa aattttcaat tccgtgctta tggagagggt
      601 ctcttatgct gcatgaaatg aggctgtaaa gcaaggagta ttcacggttt tgtgttctgc
      661 tcgaaacagc tttctctccc cctttcaaag agtgaagcac tttccccgat agtcagggga
      721 aagtgctttt tttgtgattt tttaaaggtg ccgtgcctct cttttattga agcttcgcca
      781 atccctgatc gagttggttt tgaatttctg acggcaattc atcgtaatgc tggtgcaggt
      841 aattgtattc aatctcgcct tccgggtttt cacgattgtt aacgtagagt tggactttct
      901 cgtacgtaat gaacccgctt gccgtatcga tggtgacggt tatttcctgc ctgtagggtt
      961 cctcctcctg gctgagcgta atggtcaacg caccgtcctg aatgactgcc tgggcggact
     1021 ttagcgtagt ggaatcattg agcatttgtt tgatgtgttc cagtgaagat tccagcaact
     1081 gtgggcagga ttggtcaggt attttgacca gaaggactcc gttttccgca ccagaagctc
     1141 ccacgttctg cgatacggtg ggtatcttgc tttcactgat gagaacgctg gtttctaaaa
     1201 cctgaccgtc ctcctggtag atgatctgca gaatcatcgg gcaggtcgcg gcatcgctgt
     1261 gttggaacca ccactccgcg cttgttccgt gtaaattacc cgattgagat tggatcacct
     1321 tttgctcatg ccaccaaccc ggctgtgcaa gccattgttc ccatcgaagc cgttccaatt
     1381 cgtcaagcat aatcttgata tcgtccggtt cggtgtaaac ttgtggctct tcagattcaa
     1441 ccccgttttg ggaaccgaat gaattttggt cagaagcggt cgttttcatt ccctggcatg
     1501 cggaaaagct gagcaaaatg atcagcatgc agagggacgc agtattgagt tttcttttca
     1561 tagtttctcc tagttgtttt aggtcttgac aaacctctct agtatcacta taatactagt
     1621 taactagtat gctatgacta tatgatacaa acaaatcaaa agaatgtcaa gaggtaaaga
     1681 ggtcgatatg aacaaaaata tattgaagac agctattcaa ttgagcgtcg ttgggctcat
     1741 gctgatctcc gccatgcaca tgaatattgg gaaagttgag gcgcaggctg aatcagccga
     1801 tgaaaccccc caagtgatct ctccgcaaaa tgttcaagat ttgaagctgt tgcgttggat
     1861 tggggaaggt gcctacaccg gcacaattgc ccaacaaaca gacggcaatc tgatcgctgc
     1921 tgccaccact gccggcgtaa agctgctgga tcgggagagt ggtgagcaaa ttggttttat
     1981 ccctattggg ttggaaccta ccgctctatc catttctcct gatgggaaca ccttggcagt
     2041 ggtggtgaac taccctgacg gaagtttggg aggttttatg gggcttcctg gttttaaccc
     2101 gcagatccag ttctattctt tgcctgcggg tgaaaagcaa ggggaggcga tcgacgaatt
     2161 gggggagtgc ggcgaatcga acattttggc tattgcctat tctcctgagg gctccgaact
     2221 ggtttttgaa agaaagcaca gcgctcaaaa tgatggaagg agattttgtg tactttctat
     2281 cactgagaga aagatcgtgc gcacaaagga cgtatcggat agcgctcaaa tggtaatttc
     2341 tccccaaggg gattttgtgg ctgtgattac agcaaatagc caggccgaga gcagcacggt
     2401 ctcaatctac tccaccgaag atttccacct tgcaacagag tttgaaatca accagactgt
     2461 tttttcttac aacttctcat tctcacaaga tggtcaatac cttggattga attcctatga
     2521 agaagatcag aattcaaccg tctacagctt tcaggtttgg gatctggaag atggggagtt
     2581 ggtcttttcg gaggagccta cagatgatat ggttcaatcg tttgatgtgg attccaaccg
     2641 cagtgcgcta attgtaggaa cccaattcgg ttatgtgaag atctattcac tcaacaccgg
     2701 gaaaatggaa aagcaattgg gtcctttcac ctggacaaac tactctcaga cgggaaaccc
     2761 cggcggagtc actgctgctg aaacacccgc ttccatcaag agtgtgatcg taagtcgtag
     2821 cggggagtcg ctcacggttt ctgattatat gacgacctat ggacaatcga gccacattcg
     2881 aatcttcgac atgccagatg gaggtgcagg agccgatttt gtcagtacct ccatgggtag
     2941 cgagagcctg caaattgcat tttctcccga tagcagtcag atcgcgttgg ctggctccct
     3001 ggacgggagg gtagatgttt tcaatgcgca gaatggtgag ttggctttga cgcttagtgg
     3061 gcatacccag gttgtgaatc aggtaatttt ttcaccggat ggcaacgtta ttgccactgg
     3121 ctcgaatgac aataccgttc gtctgtggaa tgcgcaaacg ggggcgttgc tcagggtctt
     3181 ggaagggcat cagggaaggg tcaatcgtct ggttttttct cctgattcca cctggttgct
     3241 ctcaggtgca gatgataatt ctctgcgacg gtggaatgta tcagatggtg aattgctgga
     3301 aacgctggaa ctgggaaacg aaaactggcg ggttgaattt ctggatattc tgaacgataa
     3361 cgtttcagtg gtatatcgca tcatcaagta tccgtctccc tatattggct ttatcaccaa
     3421 gcaaatgatc tggaatacgc aaagtggtga aagtcagaac attggcggat ctgacatttc
     3481 catttctgga ttgtccgctg ataaagacct tttcatgggc ttttcttcgg ggaagatggt
     3541 tggaacactt caggaggatg ggagcatgaa aacgattacc actttccgct ctccatatgg
     3601 caatggcgct ctcacgaagc cggcgatctc accggacaac caattggtca ttgcggggaa
     3661 tggttttgga ttgcatgcct gggagttgaa cggcagtagc ttgaattttc tggggttgat
     3721 cgctgtcagc gaaccggttc ccagttatgg ggatgaatac ctgttctcgc cagatagaaa
     3781 atatttggca tataccgctg gcggggttgc ctatctgatg ggcgtgattg agaaataaga
     3841 tttctttcca gggatgggtt tattcccatc cctggaaaca tggaaatgag ttaacccggc
     3901 gcaattggac tggataggat agctataata taaagaacga tttgaaataa caacgcttca
     3961 cacagagact ttaatggatt acatatttga cgataaccaa cccatttatc tacaaatcaa
     4021 gcagcgcatc tgtgccgata ttctacggcg taaatatagc cctggcgata aattgcccgg
     4081 catcgtggaa actgccatgc agttcaaggt caatcacaat accattcagc gagtgtatca
     4141 ggaattgatc agagaaggtg tcgctatttc aaaacgtgga gaaggcacct ttgtcactga
     4201 ggatacaaaa gttctaaaga agatggaaac agaactgcaa acgtacgcta tggaaggttt
     4261 catcgaagag atggtgcggt tgggatatca ggaaggggat ctggcgcaga tcatcgagca
     4321 gtatatccat tcccggcagg tcaaaagaaa agaggaatag tatcacatgc cgaacattta
     4381 tcgcgaagaa ctttcacgcg cgttggtgaa caaacgtttt ttgctggttt tcctgctggc
     4441 ggggatcagc tttttgtatg gattctttca aatgaaaacc atcgttgtcg agcctttgga
     4501 tgcgatcacg atctggcagg gaattttgca acgcgggtat tatggatttt ttgcctgcgt
     4561 gatggcggtg ttgccctttg ctgattcttt gagtgtcgac aagaatgaac gggtcatcaa
     4621 ccatttcttg ttgcgggtgg agtataaaaa atatatcgca gccaagttct tatccgctat
     4681 agtatcgggg gctgttgcag tgctcttgcc agctatcctg atgctgttga tttgtgcgtt
     4741 gatgtaccct gcaaaccctg ttcaaattcc cacgctctca ttcagcgtga aggaaatttt
     4801 gccgggtcag gtgatttccc ctggcaccgc attgaaactt acccctctgg ggtatctagc
     4861 cttgtgctgg ctgtttctgg cttttttcgg gatggcgtac gccatgctgg cgatgggact
     4921 atctttccat acggagaaca gcctgattgt ccttggattc ccgtttctct gctacagttt
     4981 tggctactac ttcatcccat cctcacgcca tctgcattgg ttgatttcat cggaagctgc
     5041 cctgatcccg caaggaaatt tgatatcggc tgtggttcaa tgggtcggct tcggattatt
     5101 tttttgcgcc aatgtgcttc tatttggaaa caaagagcgg caggtaatca aatgacggcc
     5161 atgaaatctc gagcgttttt aaaatatcag tctgctcatt acttcttacg ctggcgctgg
     5221 cttcttccga tcccggtcgg tttatgcctg ggatattggg ctcaaaaggt gatcagcgtg
     5281 ttgaccccgc ccttttcttc tgggctgcag ggaaatgctt tagaagcgtt catttgggct
     5341 ttcgggaaac cggagatcat ctattttgtc atatccattc tcttcatttt tctggtctcc
     5401 gacctgcttc ctgaaaactc ctatgggcag caagttcttc tgcgacttcg gtcgcgcaag
     5461 acctggtggt tggggaaaac gatttttgtt tttctctcta cgctggttta tgctttcttg
     5521 ctgatcggaa gtttctttct gccggtgctg atgaaatatc cgatctcaac ggagtggagt
     5581 gctgcggggg tgagcactta tgggatcggg ttgggatatt cccttaaaaa tggcagcccg
     5641 cttcaagctt ttgggagcat tctttccttt ctgcttatcg gatggtttgc gatcgggttg
     5701 ttcatccaga tcgtcaacct cataagccag caccgttggg ttgggttctt cagcggtgtt
     5761 ctgatcgtca tctgcgccag ccttggtgat atcaagggag gtcccattgg aggagagggc
     5821 attggttcgt tctttttact tcaaaaccat ctggaattca cccctttgtg ggcgccggtg
     5881 cgtgtcatcc cagaaatata ttcatggctg ttttggttga tatggattct cgcctgtttg
     5941 ggatcaggtt tcatgctctg taaaaaaatt gatttctttt tggtcaatca tcaggaggat
     6001 taatcacatg gataaacatc tgccaactct tgagttgcgt ggaataacga agcgttttaa
     6061 gcatatggtg gtctttgaag atatcgacct tgaaatcttg ccggggggta gcatcaacgg
     6121 gatcatcgga cc
//
LOCUS       DAOSDS010000120         6130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4621_length_6130_cov_3.729218, whole genome shotgun sequence.
ACCESSION   DAOSDS010000120 DAOSDS010000000
VERSION     DAOSDS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4621_length_6130_cov_3.729218"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="PLL88_10395"
     CDS             <1..972
                     /locus_tag="PLL88_10395"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HOJ02015.1"
                     /translation="AVPERKEWKPEYANQIYAGWVGQLAGGSFGTCIEGYTGKRIAEV
                     YGKIDSYITEPETTNDDVVYELVFLDAYERMGKRVSSEAIAMEWVKQVPFGWSAEWMA
                     LRNLNMGIFPPDSGSWFNPYSEWIGAQMRGMVCGMLAPAQPMEAARLAYIDGVISHAR
                     NGVYGEIFAAVLTALAFEMQDVHLLIETSMQFIPQKSQYHAVVYAALEKIKNGGDPVA
                     IWQEIEEELKRYNWIHAYPNIAAVLFALWLGDGDFTRTLSLLAVAGMDVDCNGGLAGT
                     IVGVMRGVPPQWADPIGDLLETYILGKEKLSIKQLAQKTAMLAEKNR"
     gene            complement(978..1313)
                     /gene="rsfS"
                     /locus_tag="PLL88_10400"
     CDS             complement(978..1313)
                     /gene="rsfS"
                     /locus_tag="PLL88_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOJ02016.1"
                     /translation="MEVARTLVAALEDKKGEDIVLMDISNISQFTDYFIICTGSSERM
                     LQSLSRAVDEAAKTNFGLTARINGKGIEGWITLDFGDLVVHLFSERHRAYYQLEDLWA
                     EGKTLLRLK"
     gene            complement(1386..1958)
                     /gene="thpR"
                     /locus_tag="PLL88_10405"
     CDS             complement(1386..1958)
                     /gene="thpR"
                     /locus_tag="PLL88_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOJ02017.1"
                     /translation="MSYRVFTALDLSEQVISDILRFRDIFEPYHPTILRWTKTDQLHL
                     TLKFVGELHESHLQPIQQQMGEIFTKVGEFQLRLQGAGIFPHPRNPRILWVGISPVPA
                     LTEIARMNEELFHAFGYPVEKRPFMPHITIARFKDDCSHRELLTFQSFWQEQKKDFQS
                     QQIMDHLTFYQSTLTRQGPTYRIIARVKFH"
     gene            2050..2227
                     /gene="ssrS"
                     /locus_tag="PLL88_10410"
     ncRNA           2050..2227
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLL88_10410"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2304..2945
                     /locus_tag="PLL88_10415"
     CDS             2304..2945
                     /locus_tag="PLL88_10415"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOJ02018.1"
                     /translation="MTLVYKLIMKSGPEPEKTYKLEKDEFVMGRELINDLIINDPEIS
                     RRHARLFKKSDRYFVEDLNSTNGTFIAGERISRPVRLKSGDTLVLGKGIAFEFLAEEI
                     EEVPAVKEVAEGMVEKQEGTKEIKRTALNASGKNEKRLKAKQKPLEGAKKATASSVGE
                     KLRGKPTWLIVLAIVLLFLLVFCVIPLLIVEWTDQWCNFFGTFFNQIQPGVCP"
     gene            2970..4205
                     /locus_tag="PLL88_10420"
     CDS             2970..4205
                     /locus_tag="PLL88_10420"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ02019.1"
                     /translation="MNQKQASSPKWAPATKSIIIVILLIIASALIIRFSSLLPIAVGG
                     LIFAAILEPAVRFLNRKLKIPWGLGTAIMIILLVLVFLGVIVWGSVSIVEQIQGLINF
                     LLSITTNINQFLQNLSTTVLKIGPFHLDLSIIEWGAVGEKILEYAQPILSGIGNSIGG
                     LASGAFNFFWKFFLTMIITSLILSERKHQKSSFFFHIPGYEDDARHFQREIQIIWNAF
                     FKGQGKVFFVRICIYILLLGVLRVRYFVLLAFLAGIANFIPYIGVAVAWSVYFLVAVF
                     QGTTIFGLDPLPYAALVSLSGWLIDNIYDNVYTPRVMAGTLKLHPASVMLGALVGLDL
                     FGVLGMILASPLLATAKLFLRYIYRKIFDQDPWENIEQEHSSGDLKGFYKRANSRVKQ
                     FFEKILAKIRKGLRREKDD"
     gene            4198..4389
                     /locus_tag="PLL88_10425"
     CDS             4198..4389
                     /locus_tag="PLL88_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02020.1"
                     /translation="MTDTEKTSVETIAETDSYIAWKSSEPDGEDVYHLELNNVTLHFF
                     IEEWQEFLDLIQNVMKKTA"
     gene            4403..5458
                     /locus_tag="PLL88_10430"
     CDS             4403..5458
                     /locus_tag="PLL88_10430"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HOJ02021.1"
                     /translation="MQLDNFTEFHQIDMQNMLGEINGLPQQLADAYLHSANYELKGLK
                     KPDLVLVAGMGGSAIGADLLSAYCIPFCSVPIISLREYTLPAWAANHRTLVVASSHSG
                     NTEETIAVFDQALQAGLPILAVATGGEIARKAQEAQVPLWKFEHHGQPRAAVGFSFGI
                     LLALLERLDLIPPQRQLVEQTVEAMEESRENYGAHVAVVKNPVKRMAGQVVGRQAAIF
                     GSDHLAPVARRWKTQINELAKAWAQFEFLPEADHNTLAGMEKDESILEKTTVLFLKGG
                     HAHPRNLLRADLTKQEMLVSGMTVDAIDFKGGSRLAEMWDAILFGDYLAYYLAMVYDI
                     DPTPIEVLSSLKRSMKK"
     gene            5506..>6130
                     /locus_tag="PLL88_10435"
     CDS             5506..>6130
                     /locus_tag="PLL88_10435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOJ02022.1"
                     /translation="MGYRKVLHIDLDAFFCSVEELLNPELKGTVFAVGGSPESRGVVS
                     SCSYAARRLGVHSAMPMKTALLKAPDLMIVDGHYDRYRFYSKQVMGILCDTAPLVEQI
                     SIDEAFMDVTDMPQKGIEIAQTLQKRIMEQTGLPCSIGVASNKLVAKIATNIAKSTYS
                     GQGTPRAILEVPAGKEAEFLAPLPIRELWGIGKKSIPHFQKLGYATIG"
BASE COUNT         1555 a         1346 c         1654 g         1575 t
ORIGIN      
        1 gctgtgcctg aacgcaaaga atggaagcca gaatacgcta atcaaatcta tgcaggctgg
       61 gtggggcagt tggctggcgg ttcttttgga acctgcattg aaggatatac cggcaagcgt
      121 attgcagaag tgtatggaaa gatcgacagc tatatcaccg aaccagaaac gaccaacgat
      181 gatgtggtct atgagctggt ttttttggac gcctatgaaa ggatgggcaa gcgggttagt
      241 tctgaagcaa tcgccatgga gtgggtgaaa caggttccat tcggctggtc ggcggaatgg
      301 atggcgttgc gcaatttgaa tatgggcatt ttccctcctg attcgggctc gtggttcaat
      361 ccctattcag aatggatcgg cgcccagatg cgtggcatgg tgtgcggcat gcttgcgccg
      421 gcacaaccca tggaagcagc tcgtctggca tatattgatg gggtgatatc gcacgccagg
      481 aatggcgtgt atggggagat tttcgccgcc gtgttgactg cgctggcgtt tgaaatgcag
      541 gatgtgcatc tgctgataga aacgagcatg caattcatcc cgcagaaaag ccagtaccat
      601 gcggttgtct acgctgcgct tgagaagatc aagaatggtg gtgatccggt tgccatctgg
      661 caggagattg aggaggaact gaaacgctac aactggatcc acgcctatcc gaacatagcc
      721 gctgtcctgt ttgcgttatg gcttggagat ggagatttca cccgaaccct gtcgctgttg
      781 gctgtggcag gaatggatgt ggattgcaac ggtggtctgg caggcactat tgtaggcgtc
      841 atgcgcggtg ttccaccgca atgggctgat cccatcggcg atctgctgga aacatatatt
      901 cttgggaaag aaaaactttc gattaagcaa ctggcacaga aaactgccat gctggcggaa
      961 aagaaccgct agggaactta tttcaaccgc aagagggttt ttccttcagc ccacaggtct
     1021 tcaagctggt aatacgcacg atgacgttct gagaataaat gcacaaccag gtcgccaaaa
     1081 tcaagggtta tccatccctc gatgcctttg ccattgattc tggcagtgag cccaaaattg
     1141 gttttggcgg cttcatccac cgcacgggac aggctttgca gcatgcgttc gctgctgccg
     1201 gtgcagataa taaaataatc tgtaaattgg gagatattgc tgatgtccat taagacgata
     1261 tcttcgcctt ttttatcttc caacgcagcc acgagggttc gcgcaacttc caatgtttgt
     1321 tgagttccca gttttcacct catatcatag taaaagacat gcccaattct atcacgggcg
     1381 acagttcagt gaaatttgac cctggcgatg atcctgtagg tcggtccttg ccgggtgagt
     1441 gtgctctggt aaaaagtgag gtggtccatg atctgttggg attgaaaatc ctttttctgt
     1501 tcctgccaaa aagattggaa tgttaacagc tctcgatgtg agcaatcatc tttgaaacga
     1561 gcgatggtta tgtgtggcat aaaagggcgt ttctcaacag gataaccgaa agcgtggaaa
     1621 agctcctcgt tcatccgggc gatctcggtt aatgcaggaa ctggtgagat acccacccac
     1681 agaatgcggg ggttcctcgg gtgagggaag ataccggctc cctgtaagcg aagctgaaat
     1741 tcccccactt ttgtgaaaat ttctcccatc tgttgttgga tgggttgcag atgcgattca
     1801 tgcagctccc ccacaaattt cagtgtgagg tgtagctggt ccgttttcgt ccaacgcagg
     1861 attgtcggat ggtacggttc gaagatgtcg cggaagcgca ggatatcaga aataacctgt
     1921 tctgaaagat caagggcggt aaagacgcga tagctcatgg cgtgttctgg agagaatttt
     1981 atcaaatctt ccgctgtatt aaacaattcc tgaaacctag tcagcccact gaaatcatgg
     2041 tataagagca ttaccctgct gtgttcgtga tgcggcaatt aatgttttga gccaacactt
     2101 taaattatgg gttctcatct tatgttggtc acgcgatagg cgcaagccaa ggcgaagcca
     2161 acaggtcgga acccgacctg cgcaagcagg ttcaaaacta tgcagcacac ggcatggcgg
     2221 ggtctttgtt tttaacacca tctccgttga cggctgacag cgttcgttat aaactaaaga
     2281 atcagatgat tgaggaggaa aaaatgacac tagtgtataa actgatcatg aagtctggtc
     2341 ccgagcccga aaagacctat aaacttgaga aagatgaatt tgtgatgggt cgggaattaa
     2401 ttaatgatct catcatcaac gatccggaaa tttcacgcag gcatgcacgc cttttcaaaa
     2461 agagcgaccg ttattttgtg gaagatctca actccaccaa cggcactttc atagcgggcg
     2521 agaggatctc gagacccgtc cggttaaaaa gcggggatac gctcgttctt ggcaagggca
     2581 ttgcttttga gttcctggct gaggaaattg aagaagtgcc tgcggtcaaa gaagttgccg
     2641 aagggatggt ggaaaagcag gaaggaacca aagaaattaa acgaacggct ttgaacgcga
     2701 gcgggaagaa tgaaaaacgg ttaaaggcga agcaaaaacc gcttgaaggc gcgaaaaaag
     2761 caacagcttc ttcagtcggt gaaaaattac gcggcaaacc aacctggttg atcgtgctgg
     2821 cgatcgttct gttgttcctg ctggtatttt gcgtgatccc tctgcttatc gttgaatgga
     2881 ccgaccaatg gtgcaatttc tttggaacgt tctttaacca gattcaaccg ggcgtttgcc
     2941 cttagcgaag gattgagttt taaatatcta tgaaccaaaa acaagctagt tcccccaaat
     3001 gggctcctgc aacaaaaagc atcattattg tgatcctgtt gatcatcgcg tctgccttga
     3061 tcatccgttt ttccagcctc cttcctattg cggttggagg cttgattttt gcagctatcc
     3121 tggaaccagc ggtgcgcttt ttgaaccgga aattgaaaat cccctgggga ttaggcacag
     3181 ctataatgat cattttgtta gtgctggtct ttttgggcgt gattgtgtgg gggagcgttt
     3241 cgattgtaga gcagatacaa ggtttgatca atttcttgct ttcgattacc acgaacatca
     3301 accaattttt gcagaatctg tcgacgaccg tattgaagat cggaccgttc cacttggatc
     3361 tttcgatcat tgaatgggga gcggtgggtg aaaaaatact ggagtatgcc cagccgatcc
     3421 tttcaggaat tgggaattct ataggcggtc ttgcctccgg cgcatttaat ttcttctgga
     3481 aattttttct caccatgatc atcacgtcgc tcattctcag tgaacgcaag catcaaaaga
     3541 gcagcttttt ctttcatatc ccgggatatg aagacgatgc ccgccatttt cagcgtgaga
     3601 tccagatcat ttggaatgct tttttcaagg gtcaggggaa agtgttcttt gtgcgtatct
     3661 gcatttacat cctgctgctt ggggtactgc gcgtgcgtta ttttgtgttg ttggcattcc
     3721 tggcaggaat tgccaatttc atcccgtata ttggggtggc ggtagcctgg agcgtatact
     3781 ttctggtggc ggtctttcag gggaccacaa tttttggatt ggatcccttg ccgtatgcag
     3841 cgttggtaag cctttcgggt tggctcattg ataatattta cgataacgta tacacgccgc
     3901 gcgtgatggc aggtacgcta aagctgcatc cggcttcggt catgttgggc gccctggtgg
     3961 gcttggatct gtttggagtg ttgggaatga tcttggcttc tcctctgctg gcaacggcaa
     4021 aacttttcct gcgctatatt tatagaaaga tatttgatca agatccatgg gagaacatcg
     4081 aacaagaaca ctcttcaggt gacttgaaag gattttataa acgcgcgaat tctagggtaa
     4141 agcagttttt tgaaaagatt ttagcgaaaa taagaaaggg attaaggaga gaaaaggatg
     4201 actgacacag aaaaaacatc ggttgaaacc attgctgaaa ccgacagtta catcgcctgg
     4261 aaatcatctg agccggatgg agaagatgtt taccatctcg agctgaacaa tgttacgctg
     4321 catttcttca tcgaagaatg gcaagagttt ctggatctca tccagaatgt gatgaagaaa
     4381 accgcttaaa gagagtgtta ctatgcaact tgataatttt actgagttcc atcagatcga
     4441 tatgcagaat atgctgggtg agatcaatgg attgccgcaa cagttagccg atgcctacct
     4501 gcatagtgcg aactatgaac tgaaagggtt gaagaagcct gatctggtgc tggtcgccgg
     4561 catgggggga tcagccattg gggctgacct tctttcagct tactgcattc cattttgctc
     4621 ggtacccatc atcagtttaa gagaatatac tttgccggcg tgggcggcga accatcgaac
     4681 cctggtggtc gcttcttccc attcgggcaa tacggaagaa accatagcgg tttttgacca
     4741 ggcgctccag gcgggcctgc ccattctggc agtagccacg ggcggagaaa tagccagaaa
     4801 agcgcaggaa gcgcaagtgc cactgtggaa attcgaacac catggacagc cgcgcgcagc
     4861 ggttggtttt tcctttggaa tactgcttgc acttttagag cgcttagatc tcattccccc
     4921 tcagcggcaa ttggtggagc agactgtgga agcgatggag gagagccggg agaactacgg
     4981 tgcgcatgtc gctgttgtga aaaacccggt gaagcgtatg gcagggcagg tggttggcag
     5041 gcaggcagct atatttggtt cagaccatct cgcaccggta gcgcgcagat ggaagacgca
     5101 gatcaatgaa ttagcgaaag cctgggctca atttgaattc ctgcccgaag ctgaccacaa
     5161 taccctggct ggaatggaaa aagacgaaag catccttgaa aagaccaccg tgcttttcct
     5221 gaaaggcggg catgcgcatc cacgcaatct cctgcgagcc gatttgacca aacaggaaat
     5281 gctggtatct ggcatgacgg tggatgcgat cgatttcaaa ggggggtcgc gcctggctga
     5341 aatgtgggat gctatcctct tcggtgatta tttggcttac tatctggcga tggtttatga
     5401 catcgacccc acgccgatcg aggttctcag cagtctgaaa cgcagcatga aaaagtagct
     5461 caccaggcta cttttttgac gcatggtgga acacacctgc taattatggg ctaccggaaa
     5521 gtactccata ttgatctgga cgcttttttc tgttcggtgg aagaactgct gaaccccgaa
     5581 ctgaaaggca ctgtttttgc cgtgggtggt tcgccggaga gcagaggagt ggtcagttcc
     5641 tgttcctatg ctgccagaag gttgggggtt cattccgcca tgcccatgaa gaccgcgtta
     5701 ttgaaagccc ctgatttgat gatcgtcgat gggcattacg acaggtaccg cttctattcg
     5761 aagcaggtga tgggcattct ctgtgatact gcccctttgg tggaacagat ctccatagat
     5821 gaagctttca tggacgtgac cgatatgccg caaaaaggca tagagatcgc gcaaaccctg
     5881 caaaagcgca ttatggagca gacagggctg ccatgctcca tcggggtcgc ttccaataaa
     5941 ctggtggcaa aaatcgctac caatatcgcc aaatcaacct atagcgggca ggggactcca
     6001 cgggctatcc tggaggtgcc tgccggaaag gaagcagaat ttttagcgcc actgccgatc
     6061 agggaattat ggggcattgg aaagaaaagt attccgcact tccagaagct gggttatgcg
     6121 accatcggag
//
LOCUS       DAOSDS010000121         5963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4776_length_5963_cov_5.845633, whole genome shotgun sequence.
ACCESSION   DAOSDS010000121 DAOSDS010000000
VERSION     DAOSDS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4776_length_5963_cov_5.845633"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLL88_10440"
     CDS             <1..180
                     /locus_tag="PLL88_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02023.1"
                     /translation="TDKQVQKEVLQSSIALWQIEKGGVSNLAAWQNTQSILLEMGLIS
                     QPLDLEKAFTNEFLP"
     gene            183..938
                     /locus_tag="PLL88_10445"
     CDS             183..938
                     /locus_tag="PLL88_10445"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ02024.1"
                     /translation="MNHPTPFAFRLDSVSMIFPEREGELEALEQLSFEVPPFQFLCVL
                     GPSGSGKSTLLRLLAGLLQPSAGRILYAQSRQPSVGLVFQHANLMPWRDVWANIRLPL
                     ELRGVEEGQADARTRAMVELVGLQSFAHAMPATLSGGMAQRVAIARALIQDPDLLLLD
                     EPFGALDAITRERMGAELLRIWEQRRKTVVMVTHSISESLLLSDRVIVLSRRPGTVRF
                     DMPNDLPRPRSDEIRYHPHFVQLSRTLRQSIQE"
     gene            1014..1427
                     /locus_tag="PLL88_10450"
     CDS             1014..1427
                     /locus_tag="PLL88_10450"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOJ02025.1"
                     /translation="MQSDRYAGGQKRSPIQSSGGFFPKERVKMQTKVYVGNLSFDANE
                     ESLKELFSQAGEVVSANIITDKFSGRSKGFGFVEFSNAEGMTAAIEKFNGYSFMDREL
                     RVNEARPPEKRDGGGNRGGRRDNRRRDGGYNRNRY"
     gene            complement(1881..2618)
                     /locus_tag="PLL88_10455"
     CDS             complement(1881..2618)
                     /locus_tag="PLL88_10455"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ02026.1"
                     /translation="MTGLAYFDAMMPVLHKAMTTQVDNIKKLARWMADTIKSDHLIFV
                     FGAGHAGIIAEEMCYRAGSLVPTNAILSPGLTVNTRPLPMETAMERLPGLAAILYEAC
                     HIQKGDLLIIHSNSGRNTVAIEMAETAKREGVKVAALTSISHSQSVPSRHPGGVKLMD
                     VADLVIDNCGMAGDAIVAFDNFEQRCGATSTVVGAALMNAAICETVQILLNEQVTPPV
                     FQSANLDGSDAHNRKLMEYYRGRLLYL"
     gene            complement(2649..3407)
                     /locus_tag="PLL88_10460"
     CDS             complement(2649..3407)
                     /locus_tag="PLL88_10460"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HOJ02027.1"
                     /translation="MSAEVYLNTMQKIITDLDKTSMAEIKKTARAMANSIKAGRAVYY
                     YGSGHSALPVLDVFPRYGSFIGIQPIHDPRLTWTNVLGPGGTPELLWLERQEGYVANV
                     LRSYEITPQDTMLIFSHGGLNAAAIEAAMIGKKNGATVAAITSMQNYQINPAQHSSGK
                     KLADVADHVIDNGAPPEDAVVYLKNWEEPVAAASTVMVIVISMALIAETASILASEGE
                     YRPTFASPNVVTDPDHDYKVYEKYKIFHKQIIGY"
     gene            complement(3408..3875)
                     /locus_tag="PLL88_10465"
     CDS             complement(3408..3875)
                     /locus_tag="PLL88_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HOJ02028.1"
                     /translation="MMEHKIDVYAPASGRVFPLKDVGDEVFSGGLMGVGVAIEMDGSD
                     WTIYAPVDAECSAIFPTGHAVALKHENGLELLIHIGIETFREQGAFEKKVVEKQKVIK
                     GQPLICIAKSFFEKESPLVILTLLNGKNFTLTTNHKEKVIANETILFTILKEN"
     gene            complement(3856..4992)
                     /locus_tag="PLL88_10470"
     CDS             complement(3856..4992)
                     /locus_tag="PLL88_10470"
                     /inference="COORDINATES: protein motif:HMM:NF039260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HOJ02029.1"
                     /translation="MDSRRQKTGLGISLYLKDFLEDPEAAELYLDSAAKFGFSEVFTS
                     IHLPEDDFLKTSAAVREFAELVAKRGMHLAVDCSSHELKKIQDNASLKNELRRSEIAY
                     LRLDFGFQTTDVRTISEELGIHGFMLNPSIMRRAEVVEMVSALQRFSKALAIKACHNF
                     YPRPETGLGLAFFINQSAIFQELGIPVAACVPSRNKPRGPLHAGLPTLESHRHAAMHW
                     AARELMATGVVDEILIGDPFASATELADLQEAASSPTLVIRVIEEEGLSETERRILYG
                     TIHIARPDLSEWVLRSRTSREMAGMGEKLTAREAKVREANAITIDNEKYGRYSGELQI
                     VRQELPADARVNVVGRISPEDSCLLRYIADGVEFRFVQDDGTQN"
     gene            complement(5106..>5963)
                     /locus_tag="PLL88_10475"
     CDS             complement(5106..>5963)
                     /locus_tag="PLL88_10475"
                     /inference="COORDINATES: protein motif:HMM:NF014437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOJ02030.1"
                     /translation="QGLAYIVGWTGGFVLVALLLAPYLRRFGQFTIPDFLSARYGGNA
                     VRLIAVFAAILAGLLIAVIEKKVREISPKALKVHLPPFVAVLLAGVVILYVIQPIAGV
                     FSDLLTKLILMLLDAGGAVASAIIAALFLPLVMTGLHHGLTPIHLQLIEEVGFSTLQT
                     LNSMAGAGQVGAALALLLKFRNDENFKATIKGALPVGILGIGEPLIYGVTLPLFKPFV
                     TACLGAAVGGALVGLLGFGARAVYVSGILAIATNSNVLYYVICYAVAVLAGFVFTSLL
                     KFPAKKIEE"
BASE COUNT         1380 a         1740 c         1415 g         1428 t
ORIGIN      
        1 accgataaac aggtgcaaaa agaggtcctc cagagctcca tcgccctctg gcagattgaa
       61 aaaggcggcg tttccaatct ggctgcctgg cagaacaccc aatccatctt gctcgaaatg
      121 ggcttgataa gccagccgct tgacctcgaa aaagccttca ctaacgagtt cctgccctga
      181 ccatgaacca tcccacccct tttgccttcc gtttggacag cgtcagcatg atctttcctg
      241 aaagggaagg ggaactggag gcattggagc agctcagctt tgaggtccct cccttccagt
      301 tcctgtgcgt gctgggtcct tccggcagcg gcaagagcac cctgctgcgc ctgcttgccg
      361 ggctgctcca gcccagcgct ggtcgcatcc tctatgcgca gagtcggcag ccctcggtag
      421 gtctagtttt ccaacatgcc aaccttatgc cctggcgcga cgtgtgggca aacatccgcc
      481 tgccgctcga actgcgtggc gtggaggaag ggcaagccga tgctcgcacc cgcgccatgg
      541 tcgaactggt gggcttgcaa agtttcgcac acgccatgcc cgccaccctt tccggcggta
      601 tggcgcagcg cgttgccatc gcacgggcgc tcatccaaga tcccgacctg ttgctgttgg
      661 acgaaccctt cggtgcgctg gatgccatca cccgcgagcg catgggggcg gaactgctgc
      721 gcatctggga gcaacgccgc aagaccgtgg tcatggtcac ccattccatt tccgaatctc
      781 tgctgctctc cgatcgggtc atcgtgctca gccgccgccc gggaacagtg cgcttcgaca
      841 tgcccaacga tctgccccgc ccgcgcagcg atgagatccg ctaccaccct catttcgtgc
      901 agctttcgcg caccctgcgt cagtctattc aggaatagcc ctgcctggtg gttctgaaga
      961 actcatcgcc cgttaaggtc tttttccctg tataatgagc gtatctcaat tacttgcagt
     1021 ccgaccgata tgcaggtggg caaaaaagaa gcccgatcca atcgtccggc ggcttttttc
     1081 caaaagaaag ggtaaaaatg caaacaaaag tgtatgttgg caatctctca tttgatgcca
     1141 acgaagagtc cctgaaggaa ttgttttccc aggcagggga agtggtctcg gcgaacatca
     1201 tcaccgataa gttttccgga cgctcgaaag gatttggctt tgtggagttc tcaaacgcag
     1261 aaggtatgac agccgccatc gagaaattca atggctattc cttcatggat cgcgagctgc
     1321 gcgtcaacga agccagacca ccggagaagc gcgatggtgg tggtaatcgt ggggggagac
     1381 gcgacaaccg tcggcgcgat ggtggttaca accgcaaccg ctactagcgc ttccacgcac
     1441 tgtaatttca cacaggcaaa ttacccctta tcaaaacata aggggtaatt taatccctgc
     1501 gtaatcacat catccccttc cctacgtcat gcagagcata gcgatgcatc tcatcctaag
     1561 caaaaataat cacccaacaa gcccacatcc cacttaccac ccccctacgt catgcagagc
     1621 gcagcatctc accccaacca tatcaaacca gctagcaaga tagcatccca tttccctcct
     1681 cctacgtcat gcagagcgca acgatgtatc tcaccttgag taaaaataac cagccagcaa
     1741 tagagcattc caaatcttta tgaacaccga agcatctcac cttaagcaaa agtaaccacc
     1801 cagcaagccc acatcccatt tctttacaaa cccattgctc caaacctcaa cgcacactcc
     1861 gtccctcgtc aagagcgatg tcatagatac agcaaacgtc ctcgataata ttccatcagc
     1921 ttacgattat gtgcatcgct tccatccaga ttcgcgcttt gaaaaacagg cggggtcacc
     1981 tgctcgttga gcaagatctg gaccgtttcg caaattgccg cgttcatgag cgctgcgcca
     2041 acaacggtgg atgttgcgcc gcagcgctgt tcaaaattgt caaaagctac gatggcatcg
     2101 cccgccatcc cgcagttgtc gatcaccaag tccgctacat ccataagttt gaccccgcca
     2161 gggtggcgtg atggcacgct ctggctgtgg ctgatgctgg tcaaagctgc aacttttacc
     2221 ccttctctct ttgccgtttc agccatttcg atcgccaccg tgttccgtcc tgaattggaa
     2281 tgaatgatca agaggtctcc tttctggata tggcatgcct cataaagaat agccgccaac
     2341 cccggcaacc gctccattgc ggtttccatc gggagcgggc gggtgttcac ggtcagaccc
     2401 ggggataaaa tggcgttggt gggaaccaga ctgccggcgc ggtagcacat ctcttcagcg
     2461 atgattccgg catgtccggc tccaaatacg aatatcaggt gatcgctctt gatcgtatcc
     2521 gccatccaac gcgccagctt ttttatgttg tccacttgtg tggtcatcgc tttgtgcagt
     2581 acgggcatca tcgcgtcaaa ataagccaga cctgtcatac ctttttcctc tgcagtaaat
     2641 tctttcccct aatacccgat gatctgtttg tggaatattt tgtacttttc ataaacttta
     2701 taatcatgat cagggtccgt gaccacattg ggagaagcaa aggttgggcg atattcgcct
     2761 tcgctcgcca gaatgctggc ggtctcggct attaacgcca tggatatgac gatcaccatc
     2821 accgttgaag ctgccgcaac aggttcttcc cagtttttca gatacaccac tgcatcttcg
     2881 ggtggcgctc cgttatcaat gacatgatct gccacatctg ccagtttctt tcctgaggaa
     2941 tgctgggctg gattgatttg gtaattttgc atcgatgtga tcgctgctac ggtagccccg
     3001 ttctttttac cgatcatcgc cgcttcgatc gctgcggcgt tcaacccacc atgggagaaa
     3061 atgagcatgg tatcctgcgg tgtgatctcg taagaccgca ggacgttggc aacatatcct
     3121 tcctgccttt ccagccataa cagttctggc gttccgcccg gacctagaac gttcgtccag
     3181 gttaaccgcg ggtcatggat gggttggatg ccgataaagg aaccatatct cggaaagaca
     3241 tccagaaccg gcagcgccga atgtcccgac ccgtaataat agaccgctct gcccgctttg
     3301 atgctgtttg ccatggcacg ggctgtcttt ttgatttctg ccatactggt cttgtccaga
     3361 tcagtgatga ttttttgcat tgtgttgaga taaacttctg cagacattca attctccttg
     3421 aggattgtga ataaaatcgt ctcattcgca atcacttttt ccttgtgatt tgttgtcaat
     3481 gtgaaatttt taccgtttag cagcgtcaag atgaccaggg ggctttcttt ttcaaagaag
     3541 cttttcgcga tgcagatcag aggttgcccc ttgatgacct tctgtttttc caccaccttc
     3601 ttttcaaacg ctccctgctc cctgaaggtt tcaatgccga tgtggattaa cagttccagc
     3661 ccgttttcat gtttcagggc taccgcatgc ccggttggaa agatggcaga acattccgcg
     3721 tctacaggcg cgtagatggt ccaatctgaa ccgtccattt cgatcgccac accaacaccc
     3781 atcaaaccgc cggagaaaac ctcatcccca acgtccttta aagggaatac tcttccactg
     3841 gctggcgcat acacgtcaat tttgtgttcc atcatcctgc acgaacctga actccacgcc
     3901 atcggcaata tagcgcagca aacaactatc ttccggggat atccttccca ctacgttcac
     3961 tcttgcatcg gcgggcagtt cctgccgcac aatttgcagt tcgcccgaat agcgcccgta
     4021 tttttcatta tcgatcgtga tcgcgttggc ttctcgcact tttgcttctc tggcagtcag
     4081 tttttcaccc atgccagcca tctcccgcga tgtgcgcgag cgcagcaccc actccgatag
     4141 gtcagggcgc gcgatgtgga tcgtgccata cagtatcctt ctttccgttt ctgaaagacc
     4201 ctcttcttcg ataaccctga tcaccagcgt cggcgagctg gcagcttctt gtagatctgc
     4261 cagttccgtt gcagacgcga acggatctcc tatcaggatc tcatccacca cgcctgttgc
     4321 catcaactct cgggcagccc agtgcattgc tgcatgccgg tgactttcca gcgtgggcag
     4381 ccccgcatgc agcggaccgc gcggtttatt tcgcgaagga acgcaagccg ccacgggaat
     4441 tcccagctct tgaaaaatgg cggattggtt gatgaagaac gccaacccca aacctgtttc
     4501 cggtcgcgga taaaaattgt gacacgcttt gatcgccagc gccttcgaaa accgctgtaa
     4561 ggcgctcacc atctccacaa cctccgctct cctcatgatc gaagggttca acatgaagcc
     4621 gtgaataccc agctcttcgc tgatggtgcg cacatcagtc gtttgaaaac caaaatcaag
     4681 ccgcagatac gcgatttctg atcgcctcag ttcatttttc agcgacgcat tgtcttggat
     4741 cttttttagt tcatgcgaag aacaatccac agccaagtgc atgccccttt ttgctacaag
     4801 ctccgcgaat tctcttacgg ctgcgcttgt tttcaaaaaa tcatcctcag gtaaatggat
     4861 cgaggtgaaa acttcgctga aaccgaactt tgcagcgcta tcaaggtaca gttccgcggc
     4921 ttctgggtct tccagaaaat cttttaaata taaagagatg cctagccctg ttttttgtcg
     4981 cctggagtcc ataaattctt ccgtaaaatg cttatgttat actcatatgc acagagtctg
     5041 gagctaatcg aagcagtttg ccgtaaacag ccatccgatc agtccaggct ctgtctatag
     5101 atgaactatt cttctatctt ctttgccggg aatttaagta aggaagtgaa cacgaaccca
     5161 gccagtactg caaccgcgta gcaaatgacg taatacaaca cgttcgagtt ggtcgcgatc
     5221 gccaggatgc cagagacata cactgcccgg gcgccaaaac ctagcagccc caccagcgcg
     5281 ccgcccaccg cagcacccag gcaagctgtc acaaagggtt tgaacagcgg cagcgtgaca
     5341 ccatagatga gcggttcgcc aatgcccagg atccctaccg gtaatgcgcc tttgatggtc
     5401 gctttgaagt tttcgtcgtt gcggaatttc agcagcagag ccaatgctgc gcccacctgc
     5461 ccggctccag ccatgctgtt gagtgtttgc agggttgaaa atccgacttc ttcgatcagt
     5521 tgcaggtgaa tgggggtgag accatgatgc agcccggtca tcaccagcgg caggaacaat
     5581 gcggcaatga tggcgctcgc cactgccccg cctgcatcca acagcatgag gatgagtttg
     5641 gtcagcaggt cgctgaacac gcccgcgatc ggttgaatga catacaagat gaccacacca
     5701 gccagcagca ccgcgacaaa gggaggaagg tgaactttga gggctttcgg ggatatttct
     5761 ctcactttct tttcaatcac cgcgatcaac aagccagcca gaatggcggc aaacactgca
     5821 atcagcctca ccgcgttacc accataacgg gcactcagga agtctggaat cgtgaattgc
     5881 ccgaaacgcc gcagataagg cgccagcagc aacgccacca gcacgaaacc gccggtccag
     5941 ccaacgatgt aggccagccc ctg
//
LOCUS       DAOSDS010000122         5849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4876_length_5849_cov_3.944425, whole genome shotgun sequence.
ACCESSION   DAOSDS010000122 DAOSDS010000000
VERSION     DAOSDS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5849
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4876_length_5849_cov_3.944425"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..418)
                     /gene="rpmB"
                     /locus_tag="PLL88_10480"
     CDS             complement(236..418)
                     /gene="rpmB"
                     /locus_tag="PLL88_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOJ02031.1"
                     /translation="MARCEICGKTTTFGHNRSFSQRATERKFRPNLQKTTVVENGKAV
                     KKVVCAKCLKTMVKDR"
     gene            554..913
                     /locus_tag="PLL88_10485"
     CDS             554..913
                     /locus_tag="PLL88_10485"
                     /inference="COORDINATES: protein motif:HMM:NF015721.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOJ02032.1"
                     /translation="MSVTENQLGNIYISYHAIAVIAFQAALESYGVVGLAYEGVARGI
                     TKLFQKDPTSGVVVHYNDDGSISVDMYLVIEYGTRISSVANSVASNVKFQIEKSTGIS
                     VKDVNIHVRGLRVSDPD"
     gene            987..2636
                     /locus_tag="PLL88_10490"
     CDS             987..2636
                     /locus_tag="PLL88_10490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOJ02033.1"
                     /translation="MSCTKINGQDLKKYFYAGLTWLKTNQPLVNSLNVFPVPDGDTGT
                     NMFLTLKTAYAEIESATETNVGTIAKKFAHGALMGARGNSGVILSQILRGFARALDER
                     EELTADLLVSALAESRNTAYKGVVRPVEGTILTVIKDIAREAEQSRQNTDDLLKIFEN
                     MVVSADASVKRTPELLPILKQAGVVDSGGKGLFIIFEGIFRFIKGESIDVAEEKVVTL
                     SLEQMESEELNESIEAGQDFEVVVDFEPEANFNLSAFYDALEKVGTSIQVGEGEDLYR
                     MHIHVPKEKKYDPIDLVMGYGTIKKVYIENLIEQMKEQKKSKASVPTVVPPKIKEGQI
                     GVVAVSPGEGITRIFYSLGLAAVIQGGQTMNPSTEEIVSAFKDLPTDKIVILPNNKNI
                     HLAAQSAGAMTDKKVRVVPTCSIPEGLTAALMIDPDGDLDAVVESMNEIMKDVDSGEI
                     TTATRSITVNGIDVKSGEVISLLNDTLVGSADTLEKACDNLLQAVDLHEKEHLTILYG
                     NNVTKKQVDSVMDHLKREYPDLELELHEGGQPFYQFIIAIE"
     gene            2687..5071
                     /gene="recG"
                     /locus_tag="PLL88_10495"
     CDS             2687..5071
                     /gene="recG"
                     /locus_tag="PLL88_10495"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOJ02034.1"
                     /translation="MHASFLELFRFLKLEIKKGFNNKAMVGGMETMIPAWATRALAAG
                     ADEVLVGQIVSVFEPYATSQVDGRKAIIRSALSLIRQENPETEKGNSPLVEKSFSQPL
                     SYQPKAVFAPLTVIQGIGEKMAQKMKKLNLSTIYDLLHYYPRRYNDYSRLTPIRDLQP
                     GAEVTVSGAIQSIQKRKARNSRLQIVESIIGDGSGHLFITWFNQPWLATRLNIGTQIV
                     ASGKIDTYLGRVCLNNPEWEPVNADHLHTNRIVPVYPLTAGIQQKWLRERIYQAIQYW
                     AERIKDYLPPSILSAERLCSLNQALESIHFPRSQSDLEKARERLSFDELFFMQLGVIR
                     QKREWQANVSGTYQIDQSLLESEIRNLPFLLTASQRDALGDIQTDLRSGRQMNRLLQG
                     DVGSGKTVIARFAMEIVFRCGKQAAFLAPTAILAQQHFETISAMLFNDQVLSKNEIAL
                     LIGDTPEKQKEHIRQDLASGLIKLVIGTHALLEGPVNFHDLQIVVIDEQHRFGVAQRA
                     ALRSKGKNPHILIMSATPIPRSLNLTIYGDLDISILSDMPGGRIPVKTYLLHPQQKAV
                     AYEKISNQIEMGHQAFVIFPQIEGAEEETFAPAVIPGYEELVNKIFPHYKIGLLHGKL
                     KQEEKDTILQQFRDRELNILASTTVIEVGMDIPNATIILIEGANHFGLAQLHQLRGRV
                     GRGNVESYCYLVPDQDTALENDRLRKMTETNDGFELAEFDLKTRGPGDFFGTRQSGYG
                     GLKLANISDIKLITRAREQAQSLLERDPDLLNEENSALRFELENLWNAANGELS"
     gene            5075..5596
                     /gene="coaD"
                     /locus_tag="PLL88_10500"
     CDS             5075..5596
                     /gene="coaD"
                     /locus_tag="PLL88_10500"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOJ02035.1"
                     /translation="MTKAIFPGTFDPIHYGHIDIAQRAAKLFAKVYVAVYDRPLKSLL
                     FSPEERIDLVKRSFDEPNIEVIGYNTLTVDICKEVGAEVIVRGLRVFSDFEYEFRMAL
                     ANNRLAPEIDVVAFITSEKHTFLSSTTVREIAALNGDVSSMVPPHVVTALDVKLKKID
                     VEKRQGYSSSLRD"
     gene            5615..>5849
                     /locus_tag="PLL88_10505"
     CDS             5615..>5849
                     /locus_tag="PLL88_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02036.1"
                     /translation="MDILHLIDQLEELFNESKSFWLTNEVMIKEDRLIDLIDQMRLAV
                     PDEIKQSQQILNQKDRILAQAQEEANRTVALARE"
BASE COUNT         1674 a         1355 c         1364 g         1456 t
ORIGIN      
        1 caagtggacg gcggaatcga tcagaacacc ctgccgaccg ttcttcaagc aggggctgat
       61 tctttcgtga tcggttctgc aatttttcac aacgaagcag gcatagctgc agccgttcga
      121 caattgaaaa gtttccttaa ataaagaaaa cgattgcgtt ttttgaaatc aaaaagagct
      181 gatgccttct gcattacgct tcgggatcag ctccttttat cgtaaaggat tgattttatc
      241 gatcttttac catcgttttc aggcattttg cgcacacaac ctttttcacc gctttcccat
      301 tttccacaac ggtcgttttt tgcaaattcg gtctgaattt gcgttccgta gcccgctggg
      361 aaaaactgcg attgtgccca aaagtggttg tttttccaca aatttcacat ctagccattt
      421 tcattccctt tcaataattc gccaatcttt taaaaacaaa gtaaaataac gaggagatag
      481 tttaccatag tccgtaacta tcggcaagtt tttttgtttt atatacatgg taatcaaaga
      541 aagtatcatt gaaatgagtg taacagaaaa ccaactgggc aatatttata tttcatacca
      601 tgcgattgct gtcattgcct ttcaagccgc ccttgaatcc tacggtgtgg tgggtctggc
      661 gtacgaaggc gtggcaagag ggatcacaaa actctttcag aaagacccca catccggtgt
      721 cgtcgttcat tacaacgatg acggatccat ctcggtcgat atgtatctcg tcatcgaata
      781 cggaacaagg atatcttctg tcgccaacag cgttgccagc aacgtaaagt tccagatcga
      841 gaaatcaacc ggaatttccg taaaggatgt taacatccat gttcgtgggt tgagagttag
      901 cgaccctgat taactagaaa tcatctttga ggttcaatat ggcgcatgtc gaaaccaccc
      961 cacaacaagt ggatgagagt agaactatgt cttgcacaaa aatcaatggg caagatctga
     1021 aaaagtattt ttacgccggg ttaacctggt taaagacgaa ccaaccgctg gtgaattccc
     1081 tcaatgtgtt ccccgttcct gatggtgata cgggcaccaa tatgtttctg acgctgaaaa
     1141 ctgcctatgc agaaattgaa agcgctacgg agaccaacgt cggcacgatc gcgaaaaaat
     1201 ttgcacatgg cgctttgatg ggcgcgcgag gaaattctgg agttatctta tcgcagatct
     1261 tgcgcgggtt cgccagagct ttggatgagc gggaagagct taccgcagat ctactggtat
     1321 cggctttagc tgaaagccgt aataccgcgt ataagggcgt ggttcgtccg gttgaaggaa
     1381 ccattctgac cgtgatcaag gatattgccc gtgaagcaga acagtcgcgc cagaacaccg
     1441 atgacctgct gaagatcttt gagaacatgg ttgtcagcgc ggacgcatcc gttaaaagaa
     1501 cgcccgaatt actgccaatt ctcaaacaag ccggggttgt ggactctgga ggaaaagggc
     1561 tgttcatcat cttcgaaggg attttccgct ttatcaaggg ggaatccatt gatgtggcag
     1621 aagaaaaagt ggtcacttta tctcttgaac agatggaaag tgaagagctc aatgaatcga
     1681 tcgaagccgg gcaggatttt gaagtggtag ttgatttcga acccgaagca aacttcaacc
     1741 tctccgcatt ttacgatgcg ctggaaaaag tgggcacttc catccaggtt ggtgaaggcg
     1801 aagaccttta ccgcatgcac atccatgttc caaaagagaa aaaatatgat cctatcgacc
     1861 tggtgatggg gtatggcaca atcaagaagg tatatatcga aaatctcata gaacagatga
     1921 aagagcaaaa aaaatccaaa gcgtctgttc ccactgttgt ccctcccaag atcaaagaag
     1981 gacaaattgg tgtggtggcg gtttcacccg gtgaaggcat cacgcgcatc ttttacagcc
     2041 tggggctggc ggctgtcatc cagggcggac aaaccatgaa cccaagcacc gaagagatcg
     2101 tttctgcctt caaagacctc cctaccgata agatcgtcat cttgccaaac aacaagaaca
     2161 tccatctggc tgcccaatct gccggcgcca tgaccgataa gaaagtgcgc gttgtgccaa
     2221 cctgtagcat cccagaaggt ttaaccgccg cgctcatgat cgatccggat ggagacctgg
     2281 atgcggttgt tgaaagcatg aacgagatca tgaaagatgt ggattccggc gagatcacaa
     2341 ccgcaacccg ctccataacg gtaaatggga tcgatgttaa atctggcgag gtcatttcgc
     2401 tgctcaatga tacccttgtt gggtccgccg atacgttgga aaaagcgtgt gacaacctgc
     2461 tgcaagctgt cgatctgcat gaaaaagaac acctcaccat tctttatggc aacaatgtga
     2521 ccaagaaaca ggtggacagc gtgatggacc acctgaaaag ggagtatccc gatctggaat
     2581 tggaactgca cgaaggcgga caaccctttt atcaattcat cattgccatc gaataacaca
     2641 acgatcattc ttgaagtatg catcaatcat tgagtaaaat aactccatgc atgcttcttt
     2701 tttagagctt tttcgatttc ttaagttgga aataaaaaag gggtttaaca ataaagccat
     2761 ggtcggcggc atggaaacca tgataccagc ctgggcaacc cgcgctctcg ctgcgggagc
     2821 cgatgaggta ctcgtcgggc aaatcgtctc agtattcgag ccatacgcta ccagccaggt
     2881 agatgggcga aaagcgatca ttcgctcagc tttgagtctg atcaggcaag aaaatcccga
     2941 aacggaaaaa gggaactccc ccctcgttga aaaatcgttt tcacaaccac tttcctatca
     3001 acccaaagcg gttttcgctc ctctcaccgt cattcagggc atcggagaaa agatggcaca
     3061 aaagatgaag aagctgaatc tttcaaccat ttatgatctg cttcactatt accctcgcag
     3121 gtataacgat tattcacgcc tcacgccaat tcgcgacctg cagcctggtg cggaagtgac
     3181 cgtcagcggc gccatacagt ccattcaaaa aagaaaagcc cgcaatagcc gtctgcagat
     3241 tgtagaatcc atcatagggg atggaagcgg tcatctgttc attacctggt tcaaccaacc
     3301 ctggcttgcc acccgtttga acatcggcac gcagatcgtc gcttcaggga aaatagacac
     3361 ctatctgggc agggtatgtt tgaataatcc cgaatgggaa ccggttaatg cagaccattt
     3421 gcataccaat cgcatcgttc ctgtctatcc tttgaccgcc ggcattcaac aaaagtggtt
     3481 gcgcgaaaga atctaccagg ctatccaata ttgggctgaa aggatcaagg attacctgcc
     3541 accttcgatc ctatccgcag aacgactctg ttctctgaac caggctctgg aatccattca
     3601 ttttcccagg tctcaaagcg accttgaaaa agcgcgcgaa cgcctatctt tcgatgaatt
     3661 attcttcatg cagcttggcg tgatccgaca aaaacgcgaa tggcaagcca acgtttctgg
     3721 tacataccag atcgaccaga gcttgcttga gagtgagatc cgaaatctgc cctttttgtt
     3781 aactgcttcc caacgggatg ctctcggaga cattcaaacg gatctgcgct cggggaggca
     3841 gatgaatcgc ttactgcagg gagatgtggg atcaggaaaa accgtgatcg cccgatttgc
     3901 catggaaatc gtgtttcgtt gcggcaaaca ggcagctttt ttagcgccca cagccatctt
     3961 ggctcaacag cactttgaaa cgatcagtgc catgcttttc aatgatcagg ttctttcaaa
     4021 aaatgaaatt gcactgctca ttggcgacac gccagaaaaa caaaaagaac atatcaggca
     4081 agatcttgca tcaggtttaa taaagctcgt cattggaacg catgccctgc tagaaggacc
     4141 ggtcaatttc cacgatctcc aaattgtggt gatcgatgag caacatcgct ttggtgtcgc
     4201 tcaaagagcc gccctgcggt cgaaaggaaa gaatcctcat atcctgatca tgtcagccac
     4261 acccattcct cgttccttga atctgactat ttatggtgat ctggatattt caattctatc
     4321 ggatatgccc ggtgggcgca tccccgtaaa gacctatctc cttcatcctc aacaaaaagc
     4381 ggtagcatac gagaagatca gcaaccaaat agagatgggt catcaagcgt ttgttatttt
     4441 cccacagatc gagggagccg aagaggaaac attcgccccg gctgtcatcc ccggttacga
     4501 ggaactggta aacaaaattt ttccacacta caaaatcggg ttactgcatg gaaaactgaa
     4561 acaggaagaa aaagacacca ttctccagca gttccgcgat cgagaactga atattcttgc
     4621 ttccacaacg gtaattgaag taggtatgga cattcctaat gcaaccatca tcctcattga
     4681 gggtgcgaac cactttggat tggcgcagct acatcaactg cgcggtcgcg ttgggcgcgg
     4741 caacgtggaa tcgtattgtt atttggtgcc agaccaggat acggcgttgg agaatgatcg
     4801 gctgcgcaaa atgaccgaaa ccaacgacgg ttttgaactg gctgagtttg atctaaaaac
     4861 tcgcggtccc ggcgatttct ttgggacgcg acaatcagga tatgggggat tgaaattggc
     4921 aaatatatct gatataaagc ttataacaag agcaagggaa caagcacagt ctctgctcga
     4981 acgcgatccc gacctgttaa acgaagaaaa cagtgcgctc cgttttgaac tagaaaatct
     5041 atggaatgca gcgaatggag aactaagcta aacaatgaca aaagccatct ttcccggaac
     5101 ctttgatccc atccattatg gtcatatcga tattgctcag agagctgcaa aattatttgc
     5161 taaggtctat gtggctgttt atgatcgacc gttgaaatcc ctcctttttt cgcctgaaga
     5221 acggatcgac ctcgttaagc gcagttttga cgaaccgaat attgaagtga tcggatacaa
     5281 cacccttacg gttgacattt gcaaagaggt cggagcggaa gtcattgtgc gtggtttacg
     5341 tgttttttct gattttgaat atgaattccg catggcattg gcaaataacc gccttgcacc
     5401 cgaaattgat gtggttgctt tcatcaccag tgaaaaacat acctttttat cttctacgac
     5461 cgtgcgggag atcgcagcgt tgaatggtga tgtcagcagc atggtaccgc cgcatgttgt
     5521 tacagctttg gatgtaaaat taaagaagat tgatgttgaa aaacggcaag ggtattcctc
     5581 atcattgcgt gattagtgca aaaagggaaa aagcatggat attctacatt tgatcgatca
     5641 attggaagag ctttttaatg aaagcaagtc tttctggctg accaacgaag tgatgatcaa
     5701 ggaagatcgc ttgattgatc tgatagatca aatgcgcctg gctgtccctg atgaaatcaa
     5761 gcaatcacag cagatattaa atcaaaaaga cagaattctg gcacaagcgc aggaagaggc
     5821 aaatcgtact gttgcactcg ccagagaaa
//
LOCUS       DAOSDS010000123         5828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4901_length_5828_cov_4.780184, whole genome shotgun sequence.
ACCESSION   DAOSDS010000123 DAOSDS010000000
VERSION     DAOSDS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4901_length_5828_cov_4.780184"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PLL88_10510"
     CDS             complement(<1..1128)
                     /locus_tag="PLL88_10510"
                     /inference="COORDINATES: protein motif:HMM:NF016901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HOJ02037.1"
                     /translation="MTISKEIQLISFLLNQGSWVTTSVISDYLELSPRSVSTYISELN
                     KKYDGLILSSNKGYRILKDKACAILNAISTRRIPSNYEERKKYILEKILLSQQFPTID
                     FLSDRLCVSPSTLQNEISKLRSELSDFHLHLRIKNNKLSIIGLDRDKRRLVLDLINEE
                     LENSSFSLESIQDLFVTTDLKRIKKIVTNALKNNEYFLDEYSLLNYILHIAVFIELKG
                     NNYIANAKDHNALRINFRKVTSPHVCKIIEEIFSEIKLAYGGDFTIEDFSEVSLSMMT
                     SAVSNNLGKLHMDQLEELVGREIEKLLFEIVRSVRSTYSIDLKEDGFMIRFAFHLKNV
                     IKRVENNIKLRNSEFKSIKSDFPLIYVIAVFISNIINKKTNH"
     gene            1360..1674
                     /locus_tag="PLL88_10515"
     CDS             1360..1674
                     /locus_tag="PLL88_10515"
                     /inference="COORDINATES: protein motif:HMM:NF014326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HOJ02038.1"
                     /translation="MDTGKYAVIVIAYAGDSKSASMEAIEAARKGDFETAENLLKKSS
                     EVLLKAHEVHTKLLVNEAREGDVAISMILVHASNHFSIAEITRELAEQFVKLYKEVKN
                     GL"
     gene            1664..1984
                     /locus_tag="PLL88_10520"
     CDS             1664..1984
                     /locus_tag="PLL88_10520"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ02039.1"
                     /translation="MVYNIILICQWGASTGMLVEKMLEAAAKKNIEVAINAYPEAKLD
                     DYIGDADIVLLAPQVRFKKDAFEEKYKANNVPFMVVDTADYGMLNGEKVLNAVFEKLE
                     NERR"
     gene            2084..3352
                     /locus_tag="PLL88_10525"
     CDS             2084..3352
                     /locus_tag="PLL88_10525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HOJ02040.1"
                     /translation="MMEKLMDRIQKFIVPFSEKISSVKFLKAVAETMQIILPITVVGS
                     FAVLFAFIDIGPWQAFLASHPTVAMIFMNTHSLTLACISLYVVMVLPYRYATHLGMKE
                     SINMIPLTVAAFLLLTPTELFTAIPTAWLGHKGMFTALIIGFAVPRLSKFLVDKKVTI
                     KMPAGVPKFIEDTFAVFIACLIIIFSAAAIGKLLEATSVGSLHNVIYTVLQTPLKGIG
                     LSFPGLLITEILMTLFMFCGIHGSTAVPFFDALMQTANTENLSALAAGEPLPNIVNTG
                     LANSIQMGGLGATLGLGILLLIFAKSSRYKQLSRMAIVPQIFNIGEPLLFGIPIMLNP
                     LLFIPYMGGVLANTFIAYFSVALGIIAPFSGVNVSWTLPGILQGLLGNTNPLHGALLQ
                     VVILIVDMLIWYPFVKIIDKQALSEEKKTA"
     gene            3451..3690
                     /locus_tag="PLL88_10530"
     CDS             3451..3690
                     /locus_tag="PLL88_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02041.1"
                     /translation="MLLIGCAQATILAEKLHQHEIKGVLFKVIKQNGLSLTVEHNADD
                     DRTAKALVKEFVGSLPEIRNFYVNVQMIDESGRIL"
     gene            3687..3977
                     /locus_tag="PLL88_10535"
     CDS             3687..3977
                     /locus_tag="PLL88_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02042.1"
                     /translation="MTPKKETGNYLYLGEETFLKKLSVYYSWVAPVILLLLSCVFFYD
                     KAFITGFIILFLSVFLLPILKKHTVRIPKLIRVLIILALVILAFLSMNLYIE"
     gene            3992..>5828
                     /locus_tag="PLL88_10540"
     CDS             3992..>5828
                     /locus_tag="PLL88_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ02043.1"
                     /translation="MTKYPHLFSPLKVNTLMLKNRIIAAPMGIIPSHKIISSTNYGNM
                     SAFDKALGGTAVVHISGGIGETEQSVFAKYNRDVTREQISVIRQAGARCALELGLFSP
                     INSDHTVFGPIAGMRFDGWKMKMMSEEDMEAIIKSCATAICQARDFGFDLVTFHFAHD
                     SLCSQFLAPGFNKRTDEYGGSLENRIRFPLEAIRRIRQAAGPDFPIQMRVSRHLIVPE
                     SFDSEDMLFFVQKAAPYVDMVNVSCGMDCYYEANPHSATSVFEPHLYNVDFAARIKET
                     CDVLVSAVGAIMTPEEMEEVIASGKVDAVMVGRQLVADPFFPKKAQEGREEDIVPCLR
                     CLYCYHIATEHANVVCSVNPRFRRENRVPLKLEKAEQAKKVVVIGGGPGGIKAALTAA
                     ERGHKVILLEKSDRLGGQINCADYDDYKQDLKRYRDYLLIQVKKSSVEVRLNTEATPQ
                     LVKELQPDALIIAVGADAATPPIPGAEHARQAVDAYPELDKMKGKIVVIGGGTIGSEM
                     GLELAERGNEVHIVEITDTLNAQGNMLYRIAIRQHMERCKTLHTMTETKCKEIRKDGV
                     MVVGKDGQEQFLEADHVLLATGLRSKKELAHTFYGITPETAMVGDC"
BASE COUNT         1627 a         1173 c         1393 g         1635 t
ORIGIN      
        1 gtgattggtc tttttgttga tgatattgga aatgaagaca gcgatcacat aaatgagcgg
       61 gaagtcggac ttaatgctct tgaattcact gttccttaat ttaatgttgt tttcaaccct
      121 cttgataaca tttttcaaat gaaacgcaaa acggatcatg aatccgtctt ctttaaggtc
      181 aatggagtaa gtgcttctta cagaacgcac gatctcaaag agcaattttt caatttctct
      241 gcccaccaat tcctcaagtt gatccatgtg cagtttgccc aaattatttg aaactgcact
      301 ggtcatcatg gagagcgaga cctcggaaaa atcttcaatc gtaaaatccc cgccgtaagc
      361 aagctttatt tcgctgaaaa tttcttcgat gatcttgcag acgtgtgggg atgttacctt
      421 gcggaagttg attctaagcg cgttatgatc tttagcattt gcaatgtagt tgtttccctt
      481 aagttcaata aaaactgcaa tgtgcagaat gtaattaagc agcgagtact catccaggaa
      541 atattcattg ttctttagag cgttggttac gatttttttg attcttttca gatctgtggt
      601 aacgaacaga tcctggatgc tttccagact aaaagaagag ttttccaatt cctcattgat
      661 gaggtccaaa accaggcggc gcttatctcg atctaatccg atgatggata gtttattatt
      721 ttttatcctt aagtgaagat ggaaatcaga tagttcgctt cgcagtttag aaatctcatt
      781 ttgcagggtt gatggagaaa cgcataatct atctgataaa aaatcaatgg tcggaaattg
      841 ctgtgaaagc aagatttttt caagaatgta ctttttccgt tcttcgtaat tgctcgggat
      901 tctcctggtt gaaatagcat taagaatggc gcaagcctta tcttttaaaa ttctgtagcc
      961 cttgttcgat gaaaggatga gcccgtcata tttcttgttc aattcagaaa tataggtgct
     1021 taccgaacgc ggagacaatt caagataatc cgaaataaca gaggtcgtaa cccacgaccc
     1081 ttgattcaat aaaaaactga ttaactggat ttccttacta attgtcatca ttccgattag
     1141 gaaacgttaa gttctattgt aatgggtatt cttgccgctc aatcgaaaga catcatcttt
     1201 cttcattaat aatgaataac gcttttgtat agtttgcacc atttttgaga aaaaatttaa
     1261 ttttttttgc agagtggtca tgcaattcct aaaattgctt tcttgatttc aagaccatat
     1321 cattggacta ttcaactttt ttattcttga ggagttaatt tggatacagg taaatatgca
     1381 gtgatagtca ttgcttatgc tggagatagt aaaagtgctt ccatggaagc catagaggcg
     1441 gcgaggaagg gagattttga gactgcggaa aatttactaa aaaaaagctc agaagtcctt
     1501 ttgaaagcgc acgaagtaca taccaaacta ttggtcaatg aagcgcgaga aggagatgtg
     1561 gcgatcagta tgattctggt acacgcttca aatcattttt caatcgctga aattacccga
     1621 gaattggcag agcaatttgt gaaactttat aaggaggtca aaaatggtct ataacatcat
     1681 tctcatttgt cagtggggag ccagtacagg catgctggtg gagaagatgc tcgaagcggc
     1741 tgcgaaaaaa aatattgaag tggcaatcaa cgcatacccc gaagcaaagt tggatgacta
     1801 catcggcgat gcggatatcg tccttttggc tccacaggtt cgctttaaga aagatgcttt
     1861 cgaagaaaag tacaaagcaa acaatgtccc atttatggtc gttgataccg ccgattatgg
     1921 aatgttgaat ggcgaaaaag tactcaacgc cgtatttgaa aaattagaaa atgaaaggag
     1981 atgaacgaat agggaaatga tattttgtga atgaaaaagc tcagtgtaaa ccggatattt
     2041 tgaaaacacc gggcgaaaaa tgtataagaa tgagaaggag aatatgatgg aaaaattgat
     2101 ggatcgaata cagaagttta ttgttccctt ttccgaaaaa atatcttctg taaaattctt
     2161 aaaagctgta gccgaaacaa tgcagatcat actgccaatc acggttgttg gatcttttgc
     2221 agttttgttc gctttcattg acatcggtcc ctggcaggct tttctagcct ctcacccaac
     2281 agtggcaatg atcttcatga atacacattc attgacgctg gcttgcatct cattatatgt
     2341 agtaatggtc ttgccttacc gctatgcaac ccatttagga atgaaggaat caattaatat
     2401 gattcccctg acagttgcag cgttcttgtt gttaaccccc acggaactct tcactgctat
     2461 cccgaccgcc tggttgggtc acaaaggaat gttcacagcg ctgatcattg gattcgctgt
     2521 gccaaggctt tccaagttcc tggttgataa gaaggtgacc attaaaatgc cagcaggagt
     2581 tcccaaattc attgaagaca cgttcgcggt ttttattgct tgtctcatta ttattttctc
     2641 tgccgccgca attgggaaac tgctggaagc gacctcggtt ggtagtctcc ataatgttat
     2701 ttacaccgta ctccagacac ccctcaaagg cattggtctg tcattccccg gcttgttgat
     2761 cacggaaata ttgatgaccc tgttcatgtt ctgtggtatt catggatcaa ccgctgttcc
     2821 tttctttgat gctttgatgc aaactgcgaa tactgaaaat ctttctgcat tggctgccgg
     2881 cgaacccttg cccaatattg ttaatacagg tcttgccaat tcaatccaga tgggcggtct
     2941 gggtgccacc ctgggattag gcatcttgct gttgatcttt gccaagagtt ccagatacaa
     3001 acaactgtcg cgcatggcga tcgttccgca gatttttaac atcggcgaac ccctactgtt
     3061 tggtatcccg attatgttga accccttact gttcatcccc tatatgggcg gcgttcttgc
     3121 caacaccttc attgcctatt tctccgtcgc gcttggcatt atcgcgccct ttagcggcgt
     3181 gaatgtaagc tggacgttac caggaatcct tcagggcctg cttggaaaca cgaatccctt
     3241 gcacggtgca ttgctgcagg tggtgatctt gatagttgat atgttgatct ggtacccatt
     3301 tgtgaagatc atcgacaaac aggcgttgag cgaagaaaaa aagacagcct agacaaagag
     3361 taaaccttta ttgttcggta gtactgtaaa aacgtattaa gggaacgggg gtaacaggat
     3421 ctgttacccc tggttctctg aggagaaaaa atgttgctca ttggttgtgc acaggcaaca
     3481 atccttgctg aaaaattgca tcaacatgaa ataaaaggtg tgctgttcaa agtgattaag
     3541 cagaatggct taagcctgac ggttgaacat aatgcagacg atgatcgaac tgcaaaagcg
     3601 ctggtaaagg aatttgttgg atcgctgcca gaaataagaa atttctatgt gaatgttcaa
     3661 atgatcgatg aatctgggag aattttatga ccccgaaaaa agaaaccgga aattatcttt
     3721 acttaggaga ggaaactttc ctgaaaaaat tatcggtata ttattcatgg gtagcacctg
     3781 ttatcttatt gctactttct tgcgttttct tttatgacaa agcattcatt acgggattta
     3841 tcattctttt cctatcggta tttcttctcc ccattttaaa gaaacataca gttagaatcc
     3901 ccaaactcat tcgagtgctg atcatcttgg ctcttgtgat tctggcattt ttatccatga
     3961 acctctatat cgaataaacc aggagtttat catgaccaaa tatccacatc tgttttcacc
     4021 tctcaaggtc aatacgctga tgttgaagaa ccgcatcatc gcagcaccca tggggatcat
     4081 cccttcgcac aagatcatct ccagtacgaa ctatggaaat atgagcgcct ttgataaagc
     4141 gttgggtggt acggctgttg tacacatcag tggcgggatt ggagaaacgg aacaaagtgt
     4201 ctttgcgaaa tacaaccgcg atgttacgcg tgagcaaatt tctgtgatca ggcaagctgg
     4261 cgcacgttgt gcactggaac tgggattgtt cagcccgata aacagcgatc acactgtgtt
     4321 tggtccaatc gccggtatgc gttttgatgg ttggaagatg aaaatgatgt ctgaagaaga
     4381 tatggaagcc atcatcaaat cctgtgccac tgcaatatgc caggctagag attttggttt
     4441 tgatctggtc accttccatt ttgcacacga cagtttatgc agccagttcc tggcgccggg
     4501 ctttaacaaa cgcacggatg agtacggtgg ctcgttggag aaccgtatac gcttcccgct
     4561 ggaagccatc cgccgcatac gccaagcggc tggacccgat tttcccattc aaatgcgggt
     4621 tagccggcat ctcatcgttc ccgagtcgtt tgactctgaa gatatgctat ttttcgtgca
     4681 aaaagccgca ccctatgtgg acatggtgaa tgtctcttgt ggtatggatt gctattatga
     4741 agccaatcca cattctgcta ccagcgtatt cgagcctcat ttatacaatg tagattttgc
     4801 tgccaggatc aaagagacct gcgatgtgct ggtttcagcg gtgggtgcca tcatgacccc
     4861 tgaggagatg gaagaggtca ttgcatctgg aaaggtagat gcggtaatgg tagggaggca
     4921 gttggtcgct gacccgtttt ttccgaagaa ggcacaggaa gggagagaag aagatattgt
     4981 gccctgtttg cgttgcctgt actgctatca cattgcaaca gagcatgcca atgtggtgtg
     5041 ttccgtgaac ccaagatttc gtcgagagaa ccgggtgccg ctaaagctgg agaaagcgga
     5101 gcaggcaaag aaagtagtgg tgattggagg aggaccggga ggaataaagg cagcgctgac
     5161 ggcagcggag cgcgggcaca aagtgatctt gctggagaaa tcggatcggt tgggcggaca
     5221 gatcaactgt gccgattatg atgattacaa gcaagatctg aaaagatatc gcgattatct
     5281 actgatccag gtcaagaaat cgtcggtgga agtgcgcttg aacaccgaag cgaccccgca
     5341 attggttaaa gaacttcagc cagatgccct gatcattgcg gtgggagctg atgcggcgac
     5401 gccgcctata ccaggggcgg agcatgcgcg gcaggcagtg gatgcgtacc ctgaactgga
     5461 taagatgaag gggaagatcg tggtgatcgg agggggaacg atcggctcgg agatggggtt
     5521 ggaactggcg gaacgcggaa acgaagtgca cattgtggag atcaccgata cgctgaacgc
     5581 acaggggaac atgttgtacc ggatcgcgat ccggcagcac atggaaagat gcaagaccct
     5641 gcataccatg acggagacga agtgcaagga gatcaggaaa gatggggtaa tggtagtggg
     5701 gaaggatggg caagagcaat tcctggaagc ggatcacgtt ttgttggcta cgggcttacg
     5761 atcgaagaaa gagctggcgc ataccttcta tggcattacc cctgaaacag ctatggtcgg
     5821 tgattgcg
//
LOCUS       DAOSDS010000124         5804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4930_length_5804_cov_4.219516, whole genome shotgun sequence.
ACCESSION   DAOSDS010000124 DAOSDS010000000
VERSION     DAOSDS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4930_length_5804_cov_4.219516"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLL88_10545"
     CDS             <1..526
                     /locus_tag="PLL88_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOJ02044.1"
                     /translation="DEQILALAAAADLIEQKDPDPRPYALFKLLLETGIKKSECLNIK
                     LQHIDESDPQNPFLFVRYPEAKDRNKERKIPLTPAWMQFYKRYLAQYKPEDIVFPWSP
                     RRLEYILEDISKAAHLEKHISFSMCRWACALNAYRKGVERDLIRQRLGISKIQWREID
                     MKLQQLNQSIGSVI"
     gene            complement(519..1946)
                     /gene="tilS"
                     /locus_tag="PLL88_10550"
     CDS             complement(519..1946)
                     /gene="tilS"
                     /locus_tag="PLL88_10550"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOJ02045.1"
                     /translation="MTIEREILSNLQKAGFSPDELLLVAVSGGPDSLTLAYALHALGW
                     KIAIAHLDHQLRPESASECAFVKKAAGELGADFYHTAEDVKLAAEQGGISIEEAARSR
                     RYGFLFKTAQNICARAVLTAHTADDQVETILMHLLRGSGTRGLSGMQMVSKSPFHKTI
                     PLVRPLLRIWREDIERYCKERGLEPVQDASNQERTYLRNRIRHELIPLLETYNPSIKQ
                     SVVRLAEIVQDDYEFLEKQYERIFHDVIRKGPQNSLFISRQAFSELARGAQKAVIYRA
                     LRALLAETGDQVDSETVSAVLAFAQRPTSTHHLALPSHLHAYANDREIILTRSAALPV
                     EGKFPQCDEWLRFTLHDPFQIEIAPGFNLYGTVEPVIRYRKPSGDLLWETYLDADRIT
                     DSEALVRAHQPGERFFPLGMDGHGIKVSDFFINQKIPVTLRKRWPLMVIDGKIAWIPG
                     FQPAHALRVREGTNRVWHVQLREAK"
     gene            complement(1976..3724)
                     /gene="mutL"
                     /locus_tag="PLL88_10555"
     CDS             complement(1976..3724)
                     /gene="mutL"
                     /locus_tag="PLL88_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOJ02046.1"
                     /translation="MPIKILPESVTNKIAAGEVVENPASVVKELVENAIDAEARSIQI
                     QVEGGGKDLIAVNDDGVGIAADEVGLAITRFATNKIDTIDDLQRIRSLGFRGEALASI
                     AAVSRLRIQTRSKDETEGSELICEHGKLAGIKRKGFPQGSRFEVRDLFANVPARLKFL
                     KSDRTERSRISELVLLYALAYPQLRFSLEMEGRMVVQTAGNGNRREILASFYDVETAR
                     QMLEIQLAEQDYGIQGFCSPVGITRSNRRDIHFFVNGRLIQDVALSSALVSAYHSYLM
                     VGRYPIAAVFLEVPPAEVDINIHPAKAEVRFADGRSVAGRLARAVRLALLASSPAPAF
                     SQSLWNSNGAEDENQAMPASSAAIFPNPRSPFLSDDFQKVESVQAQPVLEFGEHVPLL
                     RVVGQVGATYIVAEGPDGLYLIDQHAAHERVLYEQLLEKRMQNQDSQLLLEPMLFTPN
                     ARQSELLASVEDTLKEIGFQLEEFGPGTYSIRAVPAFMNRTFSIRILNDALRELEEEN
                     SSAVSGEKEEILIRSICKGAAIKGGTVLSREEQEGLVRQLEACQNPRTCPHGRPTMIH
                     LSVDILERQFGRTGSR"
     gene            3783..4433
                     /locus_tag="PLL88_10560"
     CDS             3783..4433
                     /locus_tag="PLL88_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOJ02047.1"
                     /translation="MQNSFTVLLIDLDRTMYPFESGVWDAIGNRIHAFIKNNLSLSDN
                     AVLTLRMRLREQYRSTMRGLHIEFGINEQDYLEYVHDIDLTALIPPNPDLPAILAAIP
                     QKKYIFTNSTRYHAERVLRFLDVLNFFEDIIDANHISPYTKFERDAFPIALRLIGNPS
                     PADCVMVDDEEEIVQRAKEEGLKGILVNHNPQMNGYNHLQISTINQLEYALQQLTR"
     gene            4508..5722
                     /locus_tag="PLL88_10565"
     CDS             4508..5722
                     /locus_tag="PLL88_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ02048.1"
                     /translation="MNRFLKTILIVFVSLAGLSAAFYGGMWAGSGLGLVKGEQPSYLT
                     SSDPELADLFAPFFEVWDIIHEQYIDQPVDDEKLMQSAIGGLMEGLGDEHSSYMDPET
                     YQQVNAPLVGSYTGIGAWVDTSGDSLVIIAPMPGSPAEAAGIQSGDTVIAIDGKDMSG
                     VDPSVVLQSILGPADTVVTLTIVREGVDQPFDVSITRAEIEIPVIEYEMLENNIAYIY
                     LLQFSANAGEEVRSALNELLAQNPAGVILDLRDNTGGYLDAAFDVTSAFISDGLIMIE
                     ETGDGSQQTYYAYGNAVAPDIPLVVLVNGGSASASEIAAGAIQDRGRGTLVGTTTYGK
                     GSVQNWIELQGDNGAIRVTIARWLTPNGRQINKVGLDPDVEVEYTEEDAEAGIDPQLE
                     KAIEILLSGQAK"
BASE COUNT         1377 a         1615 c         1461 g         1351 t
ORIGIN      
        1 agacgagcag attctagccc tggcagccgc cgcggacctg attgaacaga aagaccccga
       61 tccgcgcccc tatgcgctct tcaagttact gttggagacc ggcatcaaga agagcgagtg
      121 cctgaacatc aaattacagc acatcgatga gagcgacccc caaaatccgt tcctgtttgt
      181 gcgctacccc gaagcaaagg accggaataa agagcgcaag atcccgttga cgcctgcctg
      241 gatgcagttt tataaacgct atctggctca gtacaaaccc gaagatatcg tcttcccctg
      301 gtcaccgcgc cgattggaat atattctgga agatatcagt aaagctgctc atctggaaaa
      361 acatatctcg ttcagcatgt gccgatgggc ctgcgcgctg aacgcttatc gcaagggtgt
      421 agaaagagac ctcatccgcc aacgcctggg catctcgaaa atccaatggc gtgagatcga
      481 tatgaagctt cagcaactga accaatcgat agggagcgtc atttagcttc gcgcaattgt
      541 acatgccaca ctctgttcgt gccttctcgc accctgagcg catgggcagg ttgaaatcct
      601 gggatccagg caatttttcc gtcaatcacc atcagcggcc atcttttacg cagcgttacc
      661 gggatttttt gattgataaa gaaatctgac actttaatcc catgaccatc catccccagg
      721 gggaaaaatc gctctccagg ctggtgcgca cgcaccagcg cttcgctgtc ggtgatccga
      781 tcagcatcca ggtacgtttc ccataacaga tcgccgctgg gctttctata ccggattacc
      841 ggttcaacgg ttccatataa attgaagccg ggagcaatct ctatctggaa tgggtcgtgg
      901 agggtgaagc gcagccattc atcgcactgt ggaaactttc cttcgaccgg gagggcggca
      961 gagcgggtaa gaatgatttc gcgatcattg gcatacgcat gcaaatggga aggcaaagca
     1021 agatggtggg tggaggttgg tcgttgagca aaagccagca cggcgcttac ggtttcagaa
     1081 tcgacctggt cacccgtctc agccagcagc gcacgcaacg ctcgataaat gaccgctttt
     1141 tgagctccgc gtgccaattc ggaaaaagcc tggcgcgaaa tgaacagcga attctgaggt
     1201 ccttttcgaa taacatcatg gaaaatgcgt tcatattgtt tttccaagaa ttcgtagtca
     1261 tcctgtacga tctctgctaa acgaaccaca ctctgcttga tcgatggatt gtacgtctcc
     1321 aatagtggga tcaattcatg tcgaatgcga ttgcgcaggt aagtacgctc ttgattgctg
     1381 gcatcttgta cgggttccaa tccgcgttct ttgcaatagc gttcgatatc ctcacgccag
     1441 atacgcagca ggggtctcac cagcgggatg gttttgtgga acgggctttt gctcaccatc
     1501 tgcattccgc tcagtccgcg cgttccgctt ccccgcagca agtgcatgag aatggtttcc
     1561 acttggtcat ccgccgtatg cgcagtcagc accgccctgg cacagatgtt ttgcgcggtc
     1621 ttgaaaagga aaccatagcg acggctgcgg gctgcttcct caatagatat gcccccctgt
     1681 tcggctgcca gtttgacatc ctctgctgta tgatagaaat cagcacccag ttctccggca
     1741 gcttttttca cgaatgcgca ttcactggct gattccggac gcagttggtg atcgagatgc
     1801 gcaatcgcta tcttccaccc cagcgcgtga agggcataag ccagcgtcag cgaatctgga
     1861 cctcccgaaa cagccaccaa taacagctca tcaggagaaa accccgcctt ctgtaaattg
     1921 ctaagaattt cccgttcgat ggtcacactg cgattatagc attcttaaaa aacgcctaac
     1981 ggctgcctgt gcgcccaaac tgccgctcca ggatgtcaac cgatagatgg atcatggttg
     2041 gtctgccatg cggacaggtg cgagggttct ggcaggcttc caactggcga acaagccctt
     2101 cttgctcttc tctcgataga acggtgcccc ctttgatggc agcccctttg caaatcgagc
     2161 ggatcaggat ctcctctttc tccccgctca ctgcggaact attctcctcc tccaattcgc
     2221 gcaatgcgtc gttgaggata cgtatggaaa aagtgcgatt catgaaagcc ggcaccgcgc
     2281 gaatggaata tgtgcccgga ccaaactctt ccagttggaa gccgatctct ttcaaggtat
     2341 cttccacaga tgccagcagt tcgctctgcc tggcattggg ggtgaatagc atgggctcca
     2401 gcagcagttg actatcctga ttttgcatgc gcttttcgag caattgttca tacagcacac
     2461 gttcgtgagc ggcatgctgg tcgatcagat atagaccatc tggtccctca gctacaatat
     2521 aggtcgcccc aacctgcccc accacgcgca acaacggaac atgttctcca aattccaaca
     2581 ccggctgcgc ctgcacgctt tcgacctttt ggaaatcatc gcttagaaaa ggtgagcgtg
     2641 gattcgggaa aatagcagcc gacgatgcgg gcattgcctg gttttcatct tctgcaccat
     2701 ttgagttcca cagcgactgg ctgaaggcag gtgccggcga ggatgccagc agcgccaggc
     2761 gaaccgcccg cgcgagcctg cctgcaacgc tccttccgtc tgcaaagcgc acttcggctt
     2821 ttgccgggtg gatgttgata tctacctcag caggcggaac ctccaggaac acggcagcga
     2881 tcgggtatct tcccaccatc aaataggaat ggtaggcgct caccaacgcc gagctgaggg
     2941 cgacatcttg tatcaaccgc ccattcacga aaaaatgaat gtcgcgccgg ttcgagcgcg
     3001 tgatgcccac cgggctgcag aaaccttgaa tgccataatc ctgctcggca agctgtattt
     3061 ccagcatctg tcgggcggtc tcaacatcat aaaaggaagc caggatttca cgccgattgc
     3121 cgttcccggc ggtttgcaca accatccgac cttccatctc taaagaaaac cgtaactggg
     3181 gataagccag cgcatacagc agcaccagct cactgatgcg gctgcgctcg gtgcgatcag
     3241 atttcaggaa tttcaagcgt gccggcacat tggcgaacag gtcgcgcacc tcaaagcgac
     3301 tcccttgtgg gaaacctttg cgtttgatgc ccgccagctt accgtgttca cagataagtt
     3361 cactgccttc cgtctcgtct ttagaacggg tttggatgcg cagcctggaa actgccgcga
     3421 tggaagccag tgcctccccg cgaaagccca gcgaacggat acgttgcaga tcatcgatgg
     3481 tatcgatctt gttggtagca aagcgcgtga tagccagccc cacctcgtct gcggcaatgc
     3541 ccaccccatc atcgttaacg gcaatcaggt ccttccctcc cccttcaact tgtatttgaa
     3601 tactgcgcgc ttcggcatcg atggcattct ctaccagttc cttcaccacc gaagctgggt
     3661 tctctaccac ttcgccggcg gcaatcttgt tggtcacgga ttcaggtaag attttgatgg
     3721 gcaatgggca cctcacaaca aattatatcc cagcgctttt cttttttcgg tatacttacc
     3781 ccatgcaaaa ttccttcact gttttactga tcgacctgga tagaactatg tacccctttg
     3841 agagcggggt gtgggatgcc attgggaatc gcatccatgc cttcattaaa aataacctga
     3901 gtctttcaga taatgctgtc ctgacgctgc gcatgcgcct gcgagagcag tacagaagca
     3961 ccatgcgcgg actgcacatt gaatttggca tcaatgaaca ggattacctg gaatatgtgc
     4021 atgacataga cctcactgcc ctcatcccac ccaaccctga tctgccggct atcctggctg
     4081 ccatccccca gaaaaagtac atcttcacca attccacccg ctatcatgcc gaacgcgtct
     4141 tgcgctttct cgacgttctc aacttctttg aagacatcat cgatgccaat cacatcagcc
     4201 cctacaccaa atttgagcgg gacgcttttc cgatcgcttt gcgtttgatc ggaaacccct
     4261 cccccgcgga ttgcgtgatg gtggatgatg aagaggagat cgtccaacgg gctaaagagg
     4321 aagggcttaa aggtattctc gtcaaccaca atccgcagat gaacgggtac aaccatcttc
     4381 aaatttccac catcaatcaa ttggagtatg ccctccagca gttaacgcga tgagattacc
     4441 cctctttcaa aatcgataaa aggcatacaa ttatgctttg gtttctttcg tatggagtgt
     4501 aggtaccatg aacagattct taaaaacaat actgatcgta tttgtgtctc tcgccgggct
     4561 gtctgccgct ttttatggcg gcatgtgggc aggaagcggg ttggggcttg tgaaagggga
     4621 acaaccaagc tacctgacca gctccgaccc cgagttggct gaccttttcg cccctttctt
     4681 tgaagtctgg gatatcatcc acgaacaata tatagaccag ccggtggacg atgagaaact
     4741 gatgcagagc gccatcggcg gcttaatgga agggctgggt gatgaacatt cttcttatat
     4801 ggaccccgaa acttaccagc aggtgaacgc ccctctggtg ggcagctata ccggcattgg
     4861 cgcctgggtc gatacttcag gcgattctct ggtcatcatc gcccccatgc cgggttcccc
     4921 cgctgaagcg gccggcattc aatctggcga tacggtcatt gccatcgatg gaaaagatat
     4981 gagcggtgtg gatcccagtg ttgtcttgca gagcattctg ggtccggcag atacggtagt
     5041 tacgctgacc attgtgcgcg aaggcgttga ccagccattt gatgtctcca tcacccgcgc
     5101 cgagatcgaa atcccggtca tcgaatacga aatgcttgag aacaacatcg cctacatcta
     5161 tctcttgcag ttcagcgcaa atgccggtga agaggtgcgc agcgccctga acgaactact
     5221 ggcacagaac ccggcgggcg tcatcctcga cctgcgtgat aacaccgggg gatatctgga
     5281 tgctgcattc gatgtaacct ctgccttcat cagcgatggt ctcatcatga tcgaggaaac
     5341 cggggatggc agccagcaaa catactacgc ttacggcaat gccgttgcgc ccgacattcc
     5401 gctggtcgtc ctggtgaatg gcggatccgc ctcggcatct gagatcgccg ccggcgccat
     5461 tcaggatcgc ggcagaggta cgctggtggg aaccaccacc tacggcaagg gctccgtgca
     5521 aaattggatc gaactgcagg gcgataacgg cgccattcgc gtgaccattg cacgttggtt
     5581 aactcccaat ggcaggcaga tcaacaaggt tggtctggac cctgatgtgg aggtggagta
     5641 caccgaagaa gacgccgagg ctggcatcga tccgcagtta gagaaagcca tcgaaatcct
     5701 actctccggg caagcaaaat gaaactgcca tggcgcttca tggcttttaa cgtaatgact
     5761 caatagaaaa agtaactttg atagtaacgt tgactcagat agaa
//
LOCUS       DAOSDS010000125         5797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4940_length_5797_cov_3.607454, whole genome shotgun sequence.
ACCESSION   DAOSDS010000125 DAOSDS010000000
VERSION     DAOSDS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4940_length_5797_cov_3.607454"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="PLL88_10570"
     CDS             complement(<1..253)
                     /locus_tag="PLL88_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ02049.1"
                     /translation="MLLEFIIALGLLILFHEAGHFLVGLLFKFQIEEFGIGYPPRLFK
                     LFTKNGVDYTVNMIPFGGFTRFKGENDPNEKNGFYEQNKW"
     gene            complement(336..671)
                     /locus_tag="PLL88_10575"
     CDS             complement(336..671)
                     /locus_tag="PLL88_10575"
                     /inference="COORDINATES: protein motif:HMM:NF037336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02050.1"
                     /translation="MPAPLCRQAILEKDFTTFASALELDSNLMHAVMLTSTPALLYWE
                     PATIAIMKKVRAWRAQGLPACYTIDAGPNVHVLCPAENASEAEGLLKSVNGVEALYSS
                     SPGSGVRLV"
     gene            complement(569..1303)
                     /gene="mvaD"
                     /locus_tag="PLL88_10580"
     CDS             complement(569..1303)
                     /gene="mvaD"
                     /locus_tag="PLL88_10580"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HOJ02051.1"
                     /translation="METKSATAIAHPNIAFIKYWGNRDDALRIPMNGSLSMNLDALST
                     TTSVEFHPRLQADDLVIDGEQAAPQAAQRVSDFLDIIRSRMPEKNKAIIVSHNNFPMG
                     AGIASSASAFAALAAAASQAAGLPMDTAALSRLARRGSGSACRSVPDGFVEWQAGSSD
                     QDSFAFSIAPADHWRLVDLIVVVSRTHKDVGSSIGHALAVTSPLQKARLADCPRRSAA
                     RLSLKRISPPLPAPLNLIATSCTPSC"
     gene            1578..2975
                     /locus_tag="PLL88_10585"
     CDS             1578..2975
                     /locus_tag="PLL88_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOJ02052.1"
                     /translation="MVTIDENSKVPLYTQLYRQIKADILSGTLKPGAKLKSSRVASAE
                     LHISRNTVELAYDQLFAEGLITSKPRTGYYVEAVDAEGLPDCETVRCEKMPKTPDRNL
                     VYDFRCGKLLLSELPCNQWQRLINRCFHDNREELALQKSVFSEMGLSTEIQKYIHHYR
                     NVNCTAEQIVVTSGTQFCLDLACRLLKSTNREQDVAMEEPGYDRSRVTFQNHGLHTYP
                     MELDQHGAMVSSLAATNSIAVYITPSHQFPTGVVMPLSRRNEFVEWAMRKNTFIIEDD
                     YNSHFQHDRRPLPALQSLCVDRVFYIGSFSDILFPCISVAYMVVPENLLDRLHALVAN
                     HTPFVPFLVQKPLELFMKEGCWESHLRRMRKSQKAKCEALVGALKNKFGERIHISGFH
                     AGLHVLVQAQWSVKEEELIRRAYQAGVGVYPTSTYWSHPERSENGTVLLNYGGISLKD
                     IPAAVDLLYRAWRRK"
     gene            3409..4593
                     /locus_tag="PLL88_10590"
     CDS             3409..4593
                     /locus_tag="PLL88_10590"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HOJ02053.1"
                     /translation="MFVSIMLMLIFVSMPSVRASAAASADHETWNDVVDEMEAILNEA
                     YDIYLTGDVEAAKDQVDVAYYGYYEKLGFEKTVMAYISGNRAATVEYQFSYVKKAMTA
                     GSPSSEVRASLDELINLLREDANQLDGKKESPLSVFLGSLLIITREGFEAIIIVGAII
                     AYLVKSGNKDKTRAVYWGSLIALAASVVMAVILNSLTAASGANQEIIEGVTMLVAVAV
                     LFYVSNWMVSKAEAAQWTRYIDDKVKSSMTRGSVFSLAFAAFLAVFREGAETVLFYQA
                     LLAGTQTYFNMVWLGLAVGCVALVIIYLLIRFLSIKLPLKPFFLGTSILLFLMSISFL
                     GSGIKELQEGNVIGVTAVPGITSVDILGIYPTLETLIPQAVLLVLTIVTFVIQIRKNK
                     KV"
     gene            4606..5223
                     /locus_tag="PLL88_10595"
     CDS             4606..5223
                     /locus_tag="PLL88_10595"
                     /inference="COORDINATES: protein motif:HMM:NF022101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HOJ02054.1"
                     /translation="MKPEKVSIFILMLVLVLSLGLAGCSTTEESDVSSGASDQQPTEG
                     AIGPGEVAGFEEFPIGDDIELGPLNVAGVYFQPVDMEPAGKSLPASESDMHLEADISA
                     LPNDLGYGVGDFVPYLTVRYELEKEDGTIIEGNFMPMSASDGPHYGANVKFGEAGTYK
                     VRFIIENPEAQGYLLHVDQETGVTGRFWTEPLVAEWEFDYVPRVW"
     gene            5325..>5797
                     /locus_tag="PLL88_10600"
     CDS             5325..>5797
                     /locus_tag="PLL88_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02055.1"
                     /translation="MLKYLIQVVQSSLTTSILLAMLFAFTHFDGKQRQKTGLLWGFVV
                     GIASAFVLAVFKHTTVLINREYFNIGILSVAILAEVLFYLLSWDVFKKRSSEFHEKVR
                     GVVYAVLAISLVLYALPDIFLYPTEFVMPGESAFSTDFLFKVIGYLGGLLIVGLAG"
BASE COUNT         1435 a         1328 c         1589 g         1445 t
ORIGIN      
        1 tccatttatt ctgttcatag aaaccattct tttcatttgg atcgttctcg cccttgaagc
       61 gggtaaaacc gccaaaagga atcatgttga cggtgtaatc cacgccattc ttggtgaaca
      121 atttgaacaa tctgggcggg tagccgatgc caaattcctc gatctggaac ttgaacaaca
      181 gcccgaccag gaaatgcccg gcttcgtgga ataagatcaa caagcccagc gcgatgataa
      241 attcaagcag cattctcttt tcctattctc gttttggtcg tttgaattat acccagaatg
      301 atcagtggaa atattggaaa tacatcagaa agcgctcaaa ccaggcgcac accgctgccg
      361 ggcgaggaag aatagagggc ttcgacgccg tttaccgatt tcagcagacc ttccgcttcg
      421 gatgcgtttt cggcaggaca gaggacgtgc acgttcggtc cggcatcgat ggtatagcag
      481 gcaggcaagc cctgtgcgcg ccaagcccgg acctttttca tgatcgcaat ggtggcgggt
      541 tcccaataaa gcaaagcagg cgtggaggtt agcatgacgg cgtgcatgag gttgctatca
      601 agttcaaggg cgctggcaaa ggtggtgaaa tccttttcaa ggatagcctg gcggcagagc
      661 ggcgcgggca atcagccagg cgggcttttt gcaggggaga ggtgactgcc agggcatgcc
      721 cgatgctgga tccaacatct ttatgggtgc ggctgaccac gacgatcaga tctaccagcc
      781 gccagtgatc ggcaggggcg atgctgaagg cgaaggaatc ctgatcggaa ctgcccgcct
      841 gccattccac gaaaccatcg ggtacagagc ggcaagctga gccggagccg cggcgcgcca
      901 ggcgtgagag ggcggcggtg tccatcggca gaccggcagc ctggctggct gcagcagcca
      961 gggcggcgaa agccgaagcg gaagaggcaa tgccggcgcc catggggaag ttattatggc
     1021 tgacgataat ggctttgttc ttttctggca tgcgagaacg gatgatgtcc aaaaaatcag
     1081 aaacgcgctg cgctgcctga ggagcggctt gctcaccatc gatgaccaga tcgtctgcct
     1141 gcaggcgggg gtgaaattct acgctggtgg tggtggagag ggcatccagg ttcatggaca
     1201 gcgagccatt catgggaatg cgcaaggcgt catcgcggtt tccccagtat ttgatgaacg
     1261 cgatgtttgg gtgggcgatg gcagtagctg attttgtttc catatgggta tgtatcaaaa
     1321 gcgatttttc tcagtttacc atgaatactt tttaatgatc tcctgtcatg gtaagactgt
     1381 ccgttaacaa gtgatctgta taaatctgtg cgcccgttgg aaaatttgct tggcaaatcg
     1441 gacgcgatcg tgatagagca caaaaagtca tcagtatttg acaacaaaga aattttcgca
     1501 ttgtataatt gtgggcaata gttagatttg tctaactatt gccattctgt aaaacacatt
     1561 gagaaaggaa tgggcatatg gtaacgatcg acgagaacag caaagtaccg ctatataccc
     1621 aattgtacag gcagatcaaa gcggacatat tgagcggaac gctcaaaccc ggagcaaaac
     1681 tcaaatcgag ccgggttgca tctgcagagc ttcatatcag caggaatacg gttgaattgg
     1741 cttacgatca gttattcgct gaaggtctca tcaccagcaa accacgcaca gggtactatg
     1801 tggaagctgt agatgcggaa ggattgccgg attgcgagac tgttcgttgc gaaaaaatgc
     1861 cgaaaacgcc ggatcgaaat cttgtttatg attttcgatg cggaaaactg cttctctctg
     1921 aacttccctg caaccagtgg caaaggttga ttaacagatg ctttcatgac aatcgggaag
     1981 agctggctct gcaaaaatcc gttttcagcg aaatgggact cagcacggaa atacaaaaat
     2041 atatccatca ttaccgcaat gtgaactgta cggctgaaca gattgttgta acaagcggca
     2101 cgcaattttg tctggacctt gcctgccgtc ttttgaaatc cacgaatagg gagcaggacg
     2161 ttgcaatgga agaaccgggc tacgaccggt ctcgcgtcac tttccagaat catggcttgc
     2221 atacctatcc catggaactt gaccaacacg gggcgatggt cagctcattg gcagccacga
     2281 atagtattgc ggtgtacatc acgccctctc accagtttcc aacgggggtt gtcatgcccc
     2341 tatccagacg gaacgaattc gttgagtggg cgatgaggaa gaacactttt attattgaag
     2401 atgactataa cagccatttt caacatgatc gcagaccgct ccctgcgctt caatccctct
     2461 gtgtagacag ggttttctac ataggcagtt tctccgacat cttgtttccg tgcatcagcg
     2521 tggcgtatat ggtcgttcct gaaaatttgc tggatagatt gcatgccctg gttgccaacc
     2581 atactccgtt cgtgcctttt ctggtgcaaa aaccgctgga actgtttatg aaagagggtt
     2641 gttgggaaag ccatttgaga aggatgagaa aaagtcaaaa agcgaaatgc gaggcgctgg
     2701 tcggtgctct gaaaaataaa ttcggagagc gcatccacat ttcgggattt cacgccggac
     2761 tgcatgtgct tgttcaagcg caatggtccg taaaagagga agaactgatc cgccgagcct
     2821 atcaagccgg cgtgggcgta tacccgactt ctacgtattg gagccaccct gaaagaagcg
     2881 aaaatgggac ggtgctgctt aactacgggg gaatctcgct aaaagatatc cctgctgcgg
     2941 ttgaccttct ttatagggcg tggcgaagga aataagtctg aaacgctgaa agaccccgac
     3001 accggttctg gtgccttaaa agtagatgat cccaaagtac gttcatcagt ggaatggata
     3061 tccttgtaaa ttgtcgattg cccaaacatg gcatgggtaa tcgatagtaa cacctctatt
     3121 atcggtgtta ctcgccgcag ataaaaagtt cgcgcacgaa tccgcagtgt aagcctgccc
     3181 tcaacccggg tcggatttga cccgcccgga tttggaacgt ttttggtcca aaacgcttac
     3241 acctcttatc gaacatgcct cagaaataaa gctggaacca tctggagcca tacaggtttt
     3301 cgcaactggc tcttgtggtt tctgttaggt tcatctaaca tagatacggt ctgctggagg
     3361 ttgccgatca tttgtagaaa ggaggtatga agatacaagg tgcgtaaagt gtttgtttca
     3421 atcatgttaa tgctgatttt cgtcagcatg ccctctgtga gggcgtctgc ggcagcgtct
     3481 gccgatcatg aaacctggaa cgatgttgta gacgagatgg aagctattct gaacgaggca
     3541 tacgacatct atttgacggg ggacgtggaa gctgcaaaag atcaggtgga tgtcgcttat
     3601 tacggatatt atgaaaagtt gggctttgaa aaaacggtaa tggcgtatat ttcaggcaac
     3661 cgtgcggcga cggtggaata ccaattcagc tatgtaaaga aagccatgac tgccggcagt
     3721 ccgagcagtg aagttagagc ctcgctcgac gaactgatca acctgctgcg agaggacgcc
     3781 aaccagcttg acggcaaaaa agaaagtccg ctgagcgttt ttctcggttc ccttctgatc
     3841 ataacaaggg agggctttga agccatcatc attgtcggcg cgatcatcgc ctatttggta
     3901 aaaagcggaa acaaagacaa aacccgggcg gtctattggg gctccctgat tgcgttggct
     3961 gcaagcgttg tgatggcggt tatcttaaac agcctgacgg ctgcgagcgg cgccaaccag
     4021 gagatcatcg agggcgtcac catgctggtt gctgttgccg tattattcta tgtcagcaac
     4081 tggatggtat ccaaggctga ggctgcccaa tggacacggt acatcgatga caaggtcaaa
     4141 agctccatga ccagggggag cgtgttttct ctggcgtttg cggcattttt ggcagttttc
     4201 cgtgagggcg cggaaacggt gctattttat caggcgttgc tggcaggcac gcaaacctat
     4261 ttcaacatgg tttggctcgg cctggcggtg ggctgcgtgg cgctggtgat catctatctt
     4321 ctgattcgct tcttgagcat caagctgccg ctgaagccat ttttcctcgg caccagcata
     4381 ttgctatttc tcatgtccat ttcctttctg ggctccggta tcaaggagtt gcaggagggc
     4441 aacgtgatag gtgtcacggc ggttccaggg attacgtcgg tagatatttt aggaatttac
     4501 cccacgctgg aaacgttgat tcctcaagcc gttctgcttg tgctgacaat cgttactttt
     4561 gtgatccaaa tcagaaaaaa taaaaaagtg taggaggtac atataatgaa acctgaaaaa
     4621 gtgtctattt ttattctcat gctggtgttg gttctatcgt tggggctcgc cggctgttca
     4681 acaacggagg agagcgacgt ctccagcggc gcttctgacc agcagcctac agaaggtgcg
     4741 ataggacccg gcgaagtggc tggctttgaa gaattcccta tcggcgatga tatcgagctc
     4801 ggtcccctga atgtggcagg cgtttatttc cagccggtcg atatggagcc tgcaggcaag
     4861 agcctgccag cttccgaatc tgacatgcac ctggaagcag acatttctgc gctgccaaat
     4921 gacctgggct atggcgtggg tgatttcgtc ccctatctga cggtcaggta tgagcttgaa
     4981 aaagaggacg gcacgatcat cgaaggaaac ttcatgccca tgagcgccag cgacggtccc
     5041 cactatggcg caaatgtcaa gtttggtgaa gccggcacct acaaagtgcg cttcatcatc
     5101 gaaaatcctg aggcgcaggg ctatctgctg catgtggatc aggaaacagg cgttaccgga
     5161 agattctgga cggaaccgct tgtggcagaa tgggagtttg attacgtccc cagggtttgg
     5221 taggctcatt ttttagattt ttttgtcgga tgaacgcgcc atgatcaggt tgcgtgcaag
     5281 acggtcatgg cgcttctttt atggtagaga ggcagtaaca caccgtgctg aaatatctta
     5341 tccaggtcgt acagagttcg cttaccacca gcattctttt ggcgatgctg tttgcgttta
     5401 cccatttcga cggcaaacaa agacaaaaaa caggattgct ttgggggttc gtggtgggaa
     5461 ttgcatctgc gtttgtattg gcggttttca agcatacgac cgtgctgatc aacagggaat
     5521 acttcaatat tggcatcttg tctgttgcga tccttgcaga agttcttttc tatctgcttt
     5581 catgggacgt tttcaaaaaa cgatcgtctg aattccatga aaaggtgcgg ggtgtggtct
     5641 atgccgttct ggcgatttcg ctggtgctct acgctctgcc cgatatcttc ctgtatccta
     5701 ccgaattcgt aatgcccgga gaaagcgcgt tcagtacgga ttttctcttc aaggtgatcg
     5761 gatacctggg aggcttgctg atcgtggggc ttgccgg
//
LOCUS       DAOSDS010000126         5749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_4999_length_5749_cov_4.088339, whole genome shotgun sequence.
ACCESSION   DAOSDS010000126 DAOSDS010000000
VERSION     DAOSDS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4999_length_5749_cov_4.088339"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLL88_10605"
     CDS             complement(<1..956)
                     /locus_tag="PLL88_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ02056.1"
                     /translation="MRNEVVLITGANGEIGHGLIKALSAQEDKRIVALDLKPLDDSLA
                     PLCDRFIQGDILDSMLLGRLMAEYDITTIYHLASILSTKAEYNPETAHKINVEGTLNL
                     LRMGVELSTWQGNSVKFIYPSSIAVYGLPDLETKHKEGKIKEWMWCNPITMYGCNKYY
                     CENLGQYYTAHYRQLARDRGKHSIDFRCIRFPGLISADTIPAGGTSDYGPEMLHSAAR
                     GEQYSCFVRPDTRIPFMAMPDAIKSLLTLENAPCSTLTRSVYNVTSFSPTASDIYEMV
                     KGAFPSARITFDVNQPRQTIVDSWPEDCDDSAAHNDWGWKPD"
     gene            1258..1443
                     /gene="rpmF"
                     /locus_tag="PLL88_10610"
     CDS             1258..1443
                     /gene="rpmF"
                     /locus_tag="PLL88_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOJ02057.1"
                     /translation="MTPLPKRKLSKGRRDRRRAHDALKAQNLVQCSNCGEMRLPHTVC
                     PNCGFYKGREIVKVEND"
     gene            complement(1489..2808)
                     /gene="hflX"
                     /locus_tag="PLL88_10615"
     CDS             complement(1489..2808)
                     /gene="hflX"
                     /locus_tag="PLL88_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOJ02058.1"
                     /translation="MSKKQLYNTNPPKEKAILVGVEFEENRSLLSMERSLEELYLLAN
                     TAGVEVVGELSQKLNKPNPSTFIGSGKVAELKELVSALKADLVIFDDELNPRNFRELE
                     EQLPPGIKVLDRTGLILDIFTLHANTSEGKLQVQLAQNEYLYPRLTRAWTHLVRQAGG
                     GGGRAGSVGGVGLRGPGETQLEDDRRKIRAQISLLKEELAKVQSHRQQTRLKRKRSNI
                     PLVALVGYTNAGKSTLLNRLTRADTYVADKLFATLDPTTRKLWLADGKQVLLTDTVGF
                     IQKLPHQLVAAFRATLEDITEADLLLHVVDISNPDAVAQWKSVVQTLVEIQADDIPML
                     TVLNKIDQDSHISALDRDFPQFKDAVCVSARTGEGIDELLTRLKQFLYETMVDISVKI
                     PYQQMNLVNLFHQNGMVLHATHEINHTLIAGKIPTRFYKLFAPYKYE"
     gene            complement(3081..3854)
                     /locus_tag="PLL88_10620"
     CDS             complement(3081..3854)
                     /locus_tag="PLL88_10620"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOJ02059.1"
                     /translation="MAYQYLHAILDFGSRIPGSSGSRNAAAYMETALEKNDWQVTMQD
                     FLFEGVPLQNIIAKKGNGETLIIIATHYDTRAAADRESDSQKQGNPVPGANDGGSGSA
                     VLLELSRVLPISADIEVWLVFFDAEDQGNINSWNWSVGAEYFVHHLPQFPSKVLIIDM
                     IGDEDLTIYQEKNSTQTLVDEIWNVADDLGYHAVFIPQEKYALLDDHLPFINAGIPAA
                     LLIDFDYPYWHTTDDTIEHVSVDSLQMIGDLLMRWISEQ"
     gene            complement(3950..5296)
                     /gene="trmFO"
                     /locus_tag="PLL88_10625"
     CDS             complement(3950..5296)
                     /gene="trmFO"
                     /locus_tag="PLL88_10625"
                     /inference="COORDINATES: protein motif:HMM:NF003739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HOJ02060.1"
                     /translation="MPMELRIIGGGLAGCEAAWQAAERGIPVTLYEMRPKMMTEVHKT
                     ALLGELVCSNSLGSTSLTTGNGLLFHEMKLFNSLICQIAEKVSVPAGSALAVDRLGFA
                     QKLTDTLLKHPRIRVVHEEVIEIPSEPCIIATGPLTSPAFIKALQNFHGVKNLFFYDA
                     IAPLIDADSIDMQIVFCASRYGENEIVSGDYLNCPMDKETYENFVRQLVHARQIELRP
                     FEREIQQGVDAGYGPFFEGCLPIEILARRGMNTLMYGPLRPVGLRRAIRGSPPYAVVQ
                     LRQDDIAKTTYNMVGFQTNLTFSEQRRLFRLIPGLQNAEFVRFGQMHRNTYLFAPSLI
                     DQNLQAKSRQDIFFAGQLIGVEGYLGNAATGLVAGINAAKMLKQEPLLTFPPEMMIGA
                     LCFYVSHASQGTFQPMKANFGLLPPLTSQMSSKKDKKMAYSGRALDLAEKISTKVL"
     gene            5372..>5749
                     /locus_tag="PLL88_10630"
     CDS             5372..>5749
                     /locus_tag="PLL88_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02061.1"
                     /translation="MYQKITVQPKNEIKHQATAHLAQTMTLLNMGNGEVEEEIQKSLA
                     ENPALEVDEVHRCVLCGSEIALGILCKNCMNNQLKQEAQYLTFVSPREFPVAEQDNGY
                     DEYFEDGTVDKQTLPEYVLRQVGM"
BASE COUNT         1463 a         1353 c         1364 g         1569 t
ORIGIN      
        1 taatcgggtt tccaacccca atcattgtgg gcggctgaat cgtcacaatc ttctggccag
       61 ctatcgacga ttgtctgccg tggttgattt acatcaaaag tgatcctggc agaaggaaag
      121 gcgcccttta ccatttcata gatatcggat gcggttggtg aaaagctggt tacgttatat
      181 acagaacgcg ttaaagtgct gcatggtgca ttttcgagcg tcagcaacga cttgatagca
      241 tcgggcattg ccatgaatgg gatgcgggta tctgggcgaa caaaacagga atactgttct
      301 cccctggcag ccgaatggag catctccgga ccataatcac tggtcccccc ggctggaatg
      361 gtatctgcgg agatcaatcc cggaaagcgg atgcagcgga agtcaatgga atgcttgcct
      421 cgatcgcggg caagctggcg ataatgagcg gtgtaatatt gcccaaggtt ctcgcagtag
      481 tacttattgc agccgtacat ggtgatggga ttacaccaca tccattcttt gatcttgcct
      541 tccttatgct ttgtttccaa atctggtagc ccatagactg cgattgaact gggataaatg
      601 aacttaaccg agttcccctg ccaggtggat aactccacac ccattcgaag aaggttcaat
      661 gtgccttcta cgttgatctt atgggctgtt tcagggttgt attccgcttt cgtggagagg
      721 atggatgcca agtggtaaat ggtggtgatg tcatattctg ccatcaatct gcccagtaac
      781 atggaatcaa gaatatctcc ttgaataaag cgatcgcaaa ggggcgccag gctgtcatcc
      841 aatggtttta gatcaagtgc gacgatccgt ttgtcttcct gcgctgaaag cgctttgata
      901 agcccatgtc cgatctctcc attggctccg gtgatcaata caacttcatt tctcatgcca
      961 ttttctccgc atatacatgt ttataatcga tttatggcgt agaaaaaagg ttgactttct
     1021 tatatagcga atctatttgt cacaagcatg caaaaattct gttaattccc ttttgctgtg
     1081 gtgactcatc attggctcct cccggcttct tttctctgcc ggatagtaac ttcttttttg
     1141 agtcaacgtt atccctcatg gttacttttt atttgaggca attcgatttc ttgacttaat
     1201 atgaagcttg tgcttataat agggggtcgt ttttatgttt actgatggag gtagaaaatg
     1261 actcctcttc cgaagcggaa gctatcaaaa gggcgcagag accgtcgcag agcgcatgat
     1321 gcattaaaag cgcagaatct ggttcagtgc agtaattgcg gagaaatgcg attgccgcat
     1381 actgtttgcc ccaattgtgg tttttataaa gggcgcgaaa tcgttaaagt cgagaacgac
     1441 tgatcctttt taactaccgt caaagcgctg aacgttgcag cgcttttttt attcatattt
     1501 atagggtgca aaaagtttat aaaaacgagt tggaatcttg cctgctatca gcgtatgatt
     1561 gatctcatgg gtggcatgca ataccatccc gttttgatga aagaggttca ccaggttcat
     1621 ttgctggtag ggaatcttga cggagatgtc gaccatcgtt tcataaagaa actgtttcag
     1681 gcgggtgagt aattcatcga tcccttcccc tgtccttgct gaaacacata cggcgtcttt
     1741 gaactgtgga aaatcacgat cgagcgctga gatatggcta tcttgatcga ttttatttag
     1801 caccgtcagc atgggaatat catctgcctg aatctcgacc agggtctgga ccacagattt
     1861 ccattgcgcc accgcatcgg gattggaaat atccaccacg tgcagcaaga gatccgcttc
     1921 ggtaatatct tccagggttg cacgaaaagc agcgacaagc tggtggggta acttctgaat
     1981 aaaacccacc gtgtcggtga gcaagacttg ttttccatca gccaaccata atttgcgcgt
     2041 ggtaggatcg agggtggcaa agagtttatc cgccacatag gtatctgccc tggtcaaacg
     2101 gttcaacaac gtggatttgc cggcattcgt atagccaacg agcgctacca gaggaatgtt
     2161 cgatcttttt ctttttaacc gtgtctgctg gcggtgactt tgcacttttg ccaattcctc
     2221 tttcaataga ctgatctgcg cacggatctt gcgccggtcg tcttcaagct gtgtttctcc
     2281 gggtccccgc aagcctactc cgccaacact accggctcta cctccgccgc cacccgcctg
     2341 gcgaaccagg tgcgtccagg cacgggtcag gcgtggatat agatattcgt tttgagccaa
     2401 ctggacctgc aacttccctt cactggtgtt tgcatgcagc gtgaagatat ccagaatcaa
     2461 accggtgcga tcgagcactt ttattccagg gggtaactgc tcttccagtt cacgaaaatt
     2521 ccgagggttc aactcgtcgt caaagatcac caggtccgct ttcagggcag ataccaattc
     2581 tttcaattcc gcgactttgc ctgagccgat gaaggtagat ggattgggtt tgttcaattt
     2641 ttggcttaat tccccaacca cctcaacccc ggcagtgttt gccagcagat atagttcttc
     2701 cagtgagcgt tccatggaaa gtaacgaacg gttctcctca aattctacgc ctaccaggat
     2761 cgctttttct tttggaggat tggtgttgta caattgtttt tttgacatac gtttccctga
     2821 aatatagtag cttgatacct atccattggc tattgtgatt tataggaaaa ttttactaga
     2881 acattaagac aataagacac aaaaaggtaa acccggcgaa aaccgggggc gtagagctgt
     2941 ggcttgctca gccctcttat tcgttcaatg gttcgctaag agcaaatggc gttgcaacgc
     3001 tgtattcaga aaggaacggt cgattcgcga gggtgattgt ttgttccttt ccaggtgagc
     3061 aaagcacatt aaccatctca tcattgctcg gagatccagc gcattaaaag atcgccgatc
     3121 atttgtaaac tatccactga gacatgctcg atcgtatcat ctgtggtatg ccagtacgga
     3181 taatcgaaat cgatcagcag agcggctgga attccggcgt tgatgaacgg caggtgatcg
     3241 tcaagaagcg catatttttc ttgtgggatg aacactgcgt gatagccaag gtcgtcagcc
     3301 acattccata tttcatcaac gagcgtttgg gtcgaatttt tctcctggta gatggtcaga
     3361 tcttcgtcgc cgatcatgtc gatgatcagt acttttgagg ggaattgcgg tagatggtgc
     3421 acgaaatact cagcgccaac gctccaattc caactgttaa tgtttccctg gtcctcagcg
     3481 tcaaaaaata ccagccacac ctcgatatct gctgatattg gaagaacgcg actcagttcc
     3541 agcaataccg cacttcccga accaccatca ttagcgcctg gaactggatt cccttgtttt
     3601 tggctatcgc tttcccggtc tgcggcagcc cgcgtatcgt aatgcgtggc gatgatgatg
     3661 agggtctcgc cgttcccttt ctttgcaatg atgttctgca aagggacccc ttcaaacagg
     3721 aaatcttgca tggtgacctg ccaatcgttc ttttccaacg cggtttccat gtatgctgcc
     3781 gcgttccgac taccagagct ccccggaatg cgagacccga aatcgaggat ggcatgcagg
     3841 tattgatatg ccatttcacc tgaaaaaaga ttttcatctg gtgtctgtga acaaccagac
     3901 aaaataaacg gcatgaagat taaacagcct atgatgattc ttcgaatttt cataaaacct
     3961 tcgtagaaat tttttcagcc agatccaaag cccgcccgct atatgccatt tttttatctt
     4021 ttttactact catctgtgag gtgagcggtg gcagcaatcc gaagtttgct ttcatcggtt
     4081 gaaaggtgcc ttggctggca tgtgaaacgt aaaaacataa agcaccgatc atcatctcag
     4141 gcgggaaagt caataatggc tcttgcttca gcattttcgc cgcgttgatg ccagccacca
     4201 gccctgtggc tgcgttcccc aggtatcctt ccacgccaat cagttgacct gcaaaaaaga
     4261 tatcctggcg agactttgct tgcaggtttt gatcaatgag cgagggagcg aaaaggtatg
     4321 tattacgatg catttgaccg aatctgacga attctgcatt ttgcaaaccg ggaatcaatc
     4381 gaaagagtcg tctctgctcc gaaaaggtca gattggtttg aaaccccacc atgttataag
     4441 tggttttggc aatatcatcc tgacgcaact gcaccaccgc ataagggggc gatccgcgta
     4501 tagctctgcg caaacctacg ggacgtaatg gaccgtacat cagtgtattc attcctctgc
     4561 gggctaatat ctcaatgggc aaacagcctt caaagaatgg accataaccg gcatccacac
     4621 cctgctggat ttccctttca aagggcctca actcaatttg ccgggcatgt actaactgcc
     4681 tgacgaagtt ctcataggtc tctttatcca tcgggcagtt taaataatct ccgcttacga
     4741 tttcgttctc tccatagcgg gatgcgcaaa aaacgatctg catgtctatc gaatctgcat
     4801 caataagagg cgctatggca tcgtaaaaga aaagattctt tacaccatgg aaattttgca
     4861 gagctttgat gaaggcaggg gatgtcagag gacccgtagc aatgatgcaa ggctcagaag
     4921 gaatttcgat cacttcttca tggacgaccc taatcctggg atgcttcagg agggtatcgg
     4981 tgagtttttg ggcgaaaccc aatcgatcga ccgctaatgc ggatcccgct ggcacagaaa
     5041 ccttttctgc gatctgacag atcagcgagt taaagagctt catttcgtga aaaagcaaac
     5101 cgttaccagt ggttaagctg gttgaaccca aagagttgga acaaaccagt tcacccagca
     5161 gtgccgtttt atgcacttct gtcatcattt tggggcgcat ttcatataag gtgacaggga
     5221 ttccccgttc agccgcctgc caggcagctt cacatccagc caggccacca ccaatgattc
     5281 tcagttccat cggcatgaag aaaattgtat catcgatcag gttggtatga atattgcata
     5341 ggcacctctt ttgcgctata ctcgaaagca aatgtatcaa aaaatcaccg ttcaaccgaa
     5401 aaatgagatc aaacatcagg caactgctca cctggctcaa accatgaccc tgttgaacat
     5461 gggaaatggg gaggtagaag aagaaattca aaagagcctt gcggaaaacc cggcacttga
     5521 ggtggatgag gtgcaccgct gtgtgctttg cgggtcggaa atcgcgcttg gaattctctg
     5581 caagaattgt atgaacaatc aattgaaaca agaagcgcag tatctcacat tcgtatctcc
     5641 ccgtgaattc cctgttgctg agcaggataa tgggtatgac gagtattttg aggatgggac
     5701 ggtcgataaa cagacgctcc ctgaatatgt gctgcgccag gttgggatg
//
LOCUS       DAOSDS010000127         5680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5085_length_5680_cov_4.177600, whole genome shotgun sequence.
ACCESSION   DAOSDS010000127 DAOSDS010000000
VERSION     DAOSDS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5085_length_5680_cov_4.177600"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..533)
                     /locus_tag="PLL88_10635"
     CDS             complement(204..533)
                     /locus_tag="PLL88_10635"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOJ02062.1"
                     /translation="MDTKELLSAFFEAENKRDWATYREFLSPNVVWVLYSKQIKTIRG
                     IDNYLTAMMAAYKGSDNTFVCEALYQSSDGTRIVAILKNNLGERSCDIFEFSDGRIIK
                     EYEFILA"
     gene            672..1256
                     /locus_tag="PLL88_10640"
     CDS             672..1256
                     /locus_tag="PLL88_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ02063.1"
                     /translation="MMSSSKRQLQKENTRKKIIATAYQVYAKQGFTATTAVIAKEAGV
                     SHGTIFVHFPSLDELLTCLIQDFGDTLALETHKLSESNNVGELLRTYVSVLAKYEDFY
                     IRLISERSLLPEDIQMIYANIQSTVAYHFNKVIEREGENQTIKKIPIHVVFNTWMGLL
                     HYYLLNKDFFSPEMPLLERYGSELITTFLELIKK"
     gene            1273..1515
                     /locus_tag="PLL88_10645"
     CDS             1273..1515
                     /locus_tag="PLL88_10645"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ02064.1"
                     /translation="MKVCIACGMPMNEISDFPGGDTGKNYCIHCARPDGSMQSFEEKK
                     AGMISFIVKTQGFDEKAAAKIAENTMRKLPAWKEYF"
     gene            1529..1780
                     /locus_tag="PLL88_10650"
     CDS             1529..1780
                     /locus_tag="PLL88_10650"
                     /inference="COORDINATES: protein motif:HMM:NF016855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOJ02065.1"
                     /translation="MDSLSKLPNIGKVLERNLNDIGIYTQKQLVEMGGKNAFIRIKQM
                     DNGACLHMLYAIQGAIEGVKYPQLAVSTKQDLKHFFDSL"
     gene            complement(1992..3287)
                     /gene="eno"
                     /locus_tag="PLL88_10655"
     CDS             complement(1992..3287)
                     /gene="eno"
                     /locus_tag="PLL88_10655"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOJ02066.1"
                     /translation="MDAYIESITAQEILDSRGNPTVEVDIILDDGSWGRAAVPSGAST
                     GVHEALELRDGDKARYNGKGVTKAVDNVNNIIQDNLLGWDATEQRAIDAALLELDGTP
                     HKSKLGANAILGTSLAVAKAAANYLGMPLYRYIGGVYAHILPVPMMNILNGGAHTGWQ
                     STDAQEFMVMPFGAPSFAECLRWGAEIYHALKSVLKDHGYSTLVGDEGGYAPQLKANV
                     EAVEVILEAIGKAGFKAGTDVAIALDPAASELYDEEAKVYNLRREGKKLTSEQMVAFW
                     KSWIDQFPIISLEDGLAQDDWEGWKLLTKELGSRIQIVGDDLLVTNPQRVKRAIEEKA
                     CNSLLVKVNQIGSLTETIEAVTLCQRAGWTAVTSHRSGETEDATIADLAVALNTGQIK
                     TGAPARSDRVAKYNQLLRIEAELGESGTFAGWDALKIKR"
     gene            complement(3375..4997)
                     /locus_tag="PLL88_10660"
     CDS             complement(3375..4997)
                     /locus_tag="PLL88_10660"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOJ02067.1"
                     /translation="MADKKIQTKFIFFTGGVVSSVGKGLTAAALGRLLKERGFKVGVQ
                     KLDPYLNVDPGTMSPYQHGEVFVLDDGAETDLDLGHYERFIDIHLNKDCNVTTGQVYA
                     EVISKERQGDYLGGTIQVIPHITNEIKRRIMKVAESSNADFIMLEVGGTVGDIESQPY
                     LEAIRQLRNEIGRDHTFHIHVTWLPYIGATNELKTKPTQHSVRELRSMGITPNMIIAR
                     ADQPIDETLCEKIALFCDVEKKAVIPMQTLDSYYEVPLVLEKYQVGEYLLKHFNQKPL
                     KKPEWQQWNALVDAVHNPQREVCIAIVGKYVELHDAYISVREALNHAALAHKAKLRIC
                     WIHSCELEKGKHWDELKQAAGIVVPGGFGSRGVEGKIAAARYARENNIPYLGLCLGMQ
                     MMVVEFTRFHLQNEQANSTEFDEHTPLPVIDLMPDQKNITDKGGTMRLGLYPCVLTPG
                     SIAARAYQTAQVEERHRHRYEFNNAYREVLSEKGLLFSGLSPDQLLVEIAEIKGHPFM
                     LGTQFHPEFLSRPNRPHPLFKAFMQAALARSQ"
     gene            complement(5225..>5680)
                     /locus_tag="PLL88_10665"
     CDS             complement(5225..>5680)
                     /locus_tag="PLL88_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOJ02068.1"
                     /translation="EIEKFGAYVDIGAEKPGLVHISELTHGYVKTATDVVSIGDEVEV
                     KILDFNRRKHQIKLSMRALEPEPEAIEGVTITPARKPRKSKKSKSAGEKSENETPPVA
                     EPTAMEIALRAAMEKSGENSLPQTSKTNKKNKGVSEVQENILKRTLSSR"
BASE COUNT         1381 a         1332 c         1450 g         1517 t
ORIGIN      
        1 cacaaccggt gccttctgcc tttctaacat atctgtcttt aaagatacaa gggtgcgttc
       61 ctaacgcacc aaaagcacgg aaaatattca gtgggttgag aattcaccct acaaagctgc
      121 atttttgaca atacttgaat ggatagagat gaaatcatag ttttggcagg ggcatggaca
      181 acgcaacgat aaattggaat ttttcaagcc aatataaatt cgtactcttt tataatgcga
      241 ccatcggaaa actcaaagat gtcacatgaa cgttcgccca aattattctt caggatagca
      301 acaatacggg ttccatcact actttgatac aaagcttcgc atacaaaggt gttatcgcta
      361 cccttgtatg ctgccatcat ggcggtaaga taattgtcta ttcccctgat ggttttgatc
      421 tgcttggaat acaacaccca gacgacattc ggagaaagaa actctcggta tgttgcccaa
      481 tctcgcttat tctctgcctc aaaaaaagct gaaagcaact cttttgtatc catagaaaac
      541 acaactcatt tctgtctatc tacatgcaat agaaatactg tttccatcat acaacatttt
      601 cctcgcgata ttgactttcg ataaacgatg aagtataatg agtatatact cattgagtat
      661 taggtcattt tatgatgagt tcatcgaaaa gacaattaca aaaagaaaat acgcgaaaga
      721 aaattatcgc aacagcatat caggtatatg ccaaacaagg ttttactgca actacagccg
      781 ttattgcaaa agaggctggc gtatcacatg gaacaatatt tgttcatttc ccatcgctag
      841 acgaattgtt gacttgtctg atacaagatt ttggtgacac tttagcatta gagacacaca
      901 aattatcaga aagtaataat gtcggtgaat tattaagaac ttatgtgagt gttttggcta
      961 aatatgaaga tttttatatt cggcttataa gcgaaagaag tctgttaccg gaagatatac
     1021 aaatgatata tgcaaatata caatcaacgg ttgcttatca tttcaacaag gtaattgaac
     1081 gagaaggaga aaatcagact atcaaaaaaa tccccataca tgtggtattt aatacatgga
     1141 tgggattatt gcattattac ttactaaata aggatttttt ttcgcctgaa atgccgcttt
     1201 tggagcgata tggttctgaa cttataacaa cattcttgga actgataaaa aaataaaacg
     1261 gaggttatca aaatgaaagt atgtattgct tgcggtatgc caatgaatga aatatctgat
     1321 tttccgggag gtgataccgg taaaaattat tgtattcatt gcgcgcgccc tgacggttca
     1381 atgcagtcgt ttgaagaaaa gaaagcggga atgataagtt tcatagtcaa aacgcaagga
     1441 tttgatgaaa aagccgctgc taaaattgct gaaaacacta tgagaaaact tcctgcgtgg
     1501 aaagaatatt tttaaacggg cgggtttaat ggatagttta tcaaagttgc ctaacatcgg
     1561 taaggttttg gaaagaaacc tgaatgatat tgggatatat acgcaaaaac aattagtgga
     1621 aatgggaggc aagaacgctt ttattcgtat caaacaaatg gataatgggg cctgtttaca
     1681 tatgctttat gctatacagg gagcgataga gggggtaaag tatccgcaac ttgcggtgag
     1741 cacaaaacaa gatttaaaac attttttcga tagtttatag ggaggatgat aatatttttt
     1801 ataaaataga ttggctgaca caagtcaatt agtgaaaaga tatttcttcg atccaaggaa
     1861 cgaatggata ggggggcatt ggcaacgcac cgataaattt caaaaaagcg ctactgtcaa
     1921 ctatgtcaac ttttgcaata gatacaaaaa aaggagagcg atcgctctcc tctctcatat
     1981 tcgataaagt cttatcgttt gatcttgagc gcatcccagc cggcgaaggt tccactttcg
     2041 cccaactcgg cttcaatgcg caacaactgg ttgtacttcg ccacgcggtc cgagcgggcg
     2101 ggggcgccgg ttttgatctg cccggtgttg agtgccactg ccaggtcggc gatggtggca
     2161 tcttccgtct cgcccgagcg gtgcgaggtg accgccgtcc agccggcgcg ctgacagagc
     2221 gtgaccgctt cgatggtttc ggtcagtgaa ccgatctggt tgaccttgac cagtagcgaa
     2281 ttgcaggctt tctcttcgat ggcgcgcttc acgcgctgcg ggttggtgac cagcaggtca
     2341 tctcccacga tctggatgcg gctgccgagc tctttggtga gcagtttcca gccttcccag
     2401 tcatcctgag ccagtccatc ttccaacgat atgatgggga actgatcgat ccacgatttc
     2461 cagaaagcca ccatttgttc gctggtgagt tttttgcctt cgcggcgcag gttgtagacc
     2521 ttggcttctt catcgtagag ttccgaagca gccgggtcga gggcgatcgc cacatccgtg
     2581 ccggctttga aaccggcttt gccgatggct tccaggatca cttccaccgc ttctacgttg
     2641 gctttcaact gcggagcgta accgccttca tcacctacca gggtgctgta gccgtgatct
     2701 ttcaagacag atttcagcgc atggtagatc tcggctcccc agcgcaggca ttctgcaaag
     2761 gaaggggcac cgaagggcat aaccatgaat tcttgggcat cggtggattg ccagccggtg
     2821 tgtgcgccgc cgttgaggat gttcatcatg ggcacgggca gtatatgcgc gtacacgcct
     2881 ccgatatagc ggtagagggg catgcccagg tagttggcgg ctgctttggc gaccgccagg
     2941 ctggtgccga ggatggcatt ggcgcccagt ttggatttgt ggggggtgcc gtctaattcc
     3001 agcagggctg catcgatggc gcgctgctcg gtggcatccc aacccagcag gttgtcctga
     3061 atgatgttgt tgacgttgtc cacggctttg gtgacgcctt tgccgttgta gcgggctttg
     3121 tcgccgtcgc gcagttcgag cgcttcgtgc acgccggtgg atgcgccgga gggaaccgct
     3181 gcacgtcccc aacttccatc atcgagaatt atatccactt ccacagtggg atttccgcga
     3241 gaatcgagta tctcttgcgc tgtaatagat tcaatataag cgtccatctt tgatcctttg
     3301 ctttaggtga atttataatc cgacctttaa gtataatacg aaatgttttc cgtcatgccg
     3361 ttcgccctgc attttcattg agagcgtgcg agtgccgcct gcatgaacgc tttgaaaagc
     3421 gggtgcgggc ggttagggcg cgaaaggaac tcagggtgga actgcgtgcc gagcatgaac
     3481 ggatgccctt tgatctcggc gatctccacc agaagctggt cgggagagag ccccgaaaac
     3541 aacagtcctt tttccgaaag cacctcgcgg taggcgttgt tgaactcgta gcggtggcga
     3601 tggcgttctt ctacctgtgc ggtctggtaa gccctggcag cgatggaacc cggggtaagc
     3661 acgcaggggt acaaccccag gcgcatggtg ccgcctttgt cggtgatgtt cttctggtcg
     3721 ggcatcaggt cgatcaccgg caggggagtg tgttcatcga attcggtgga attggcttgc
     3781 tcgttctgca gatgaaagcg ggtgaattcc accaccatca tttgcatgcc taggcacagc
     3841 cccaaatagg ggatgttgtt ctcacgggcg tagcgtgcgg ctgcgatctt gccttcgacg
     3901 ccgcggctgc caaatccgcc cggcaccacg atgccggcgg cttgtttgag ctcatcccaa
     3961 tgcttgcctt tttccaactc gcaggaatgg atccagcaga tgcgcagttt ggctttgtgc
     4021 gccagggcag cgtggttgag cgcttcgcgc acgctgatgt aagcgtcgtg cagttcgaca
     4081 tacttgccca cgatggcgat gcacacttcc cgttgggggt tatggacggc atccaccagc
     4141 gcgttccact gctgccactc gggctttttc aagggcttct ggttgaaatg cttcagcaga
     4201 tactcgccta cctggtattt ctcgagcacc agcggcacct cataatacga atccagggtt
     4261 tgcatgggga tgaccgcttt cttttccaca tcgcaaaaca gggcgatctt ctcgcacagc
     4321 gtctcgtcga tgggctggtc ggcgcgggcg atgatcatat tgggggtgat acccatggag
     4381 cgcagttcgc gcacagagtg ctgggtgggt ttggtcttca attcgttggt cgccccgatg
     4441 tagggcagcc aggtgacatg gatatggaag gtatggtcac gcccgatctc gttgcgcaac
     4501 tggcggatgg cttcgagata gggctggctt tcgatatcgc ccaccgtgcc gcccacttcg
     4561 agcatgatga aatcggcgtt ggaggattct gccaccttca tgatgcggcg cttgatctcg
     4621 ttggtgatgt gcggaatgac ctggatggtg ccgcccaggt aatcgccctg gcgttctttg
     4681 ctgatgacct cagcataaac ctgcccggtg gtgacgttgc aatccttgtt caaatgaata
     4741 tcaataaaac gctcgtagtg ccccaggtcc aggtcggttt ccgctccgtc atccagcacg
     4801 aacacctcgc cgtgctggta ggggctcatg gtgccggggt caacgttcag gtaaggatcc
     4861 agtttctgga cgccaacttt gaagccgcgt tctttcaaca accttcccaa cgctgccgca
     4921 gtcaatccct tgccaaccga gctgaccaca ccaccggtga aaaatatgaa ttttgtctgt
     4981 atttttttgt ctgccatgtt cacttttcct gggctcatcg ctcattgggg tttaaaagaa
     5041 gaagggaggg ttgttggata agccctccct tggagcacga attaaaaatg atcatccgca
     5101 ctacacacat tggtttcatt tcattctgac cttaatcata gcatggaaaa tactaacgta
     5161 tcacgcaaca tattattaaa aaaagaaacc acagcattga atctgtgatc tcctcgatct
     5221 tgaattagcg gctgctgagg gtgcgtttga gaatgttttc ttgcacttca gaaacgcctt
     5281 tgttcttttt attagtcttg gatgtttgcg gcagggaatt ttcaccagat ttttccatcg
     5341 cagcccgcaa cgcgatttcc atggcggtag gttctgccac aggcggggtc tcattttcac
     5401 tcttctcgcc agcgcttttg gatttcttgg atttgcgagg tttgcgcgca ggggtgatgg
     5461 tcacgccctc gatggcttcc ggttcgggtt cgagcgcgcg catgctaagc ttgatctgat
     5521 gtttacggcg gttgaaatcg aggatcttga cctcgacctc atcgccaata ctgaccacgt
     5581 cggtggcggt tttcacgtac ccatgggtga gttcgctgat atgcaccagc ccgggtttct
     5641 ccgcgccgat atccacatag gcaccgaatt tttcgatctc
//
LOCUS       DAOSDS010000128         5570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5213_length_5570_cov_5.388033, whole genome shotgun sequence.
ACCESSION   DAOSDS010000128 DAOSDS010000000
VERSION     DAOSDS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5570
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5213_length_5570_cov_5.388033"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLL88_10670"
     CDS             complement(<1..182)
                     /locus_tag="PLL88_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02069.1"
                     /translation="MKFRNMKLAFLTIVLLFSCAACEAFPVFNLFESSSKESTLVTTA
                     TAGSSETPQGGTPSKIP"
     gene            complement(342..1022)
                     /locus_tag="PLL88_10675"
     CDS             complement(342..1022)
                     /locus_tag="PLL88_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOJ02070.1"
                     /translation="MCGRFTIVAGALAYQLEFNLQMDESIEHDWNAHYNIAPGQFIPA
                     VRDPWERKMEFMQWGLLPAWAKDDKAHIRLINVRAETIMEKVTFRTLMQQGQRCLIFA
                     DGFYEWKPAEKKGAPKTPYYFRLRSGKPFAFAGLWATGLTSDQKPVPTCAIITCAPNA
                     LMHAIHSRMPVIFNSTHAWEWLSQTPAAQLVSLLQPFPESEMMAYPVSLLVNNPAADA
                     PECILPGK"
     gene            complement(1206..1877)
                     /locus_tag="PLL88_10680"
     CDS             complement(1206..1877)
                     /locus_tag="PLL88_10680"
                     /inference="COORDINATES: protein motif:HMM:NF005123.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HOJ02071.1"
                     /translation="MYGRKVEKMLEQLKKEIYKCNMELPKNGLVYWTSGNVSGRDLKS
                     NLIVIKPSGVMYEEMKPEDMVVVDLDGKVVEGKLEPSSDTASHLYIYRHRSDVNGVVH
                     THSYYATAFAAVGKPIPACITAIAENFGKEVPIGAYAPIGGEEIGAEVVHSIGDSTAI
                     IMENHGVFTIGSSAHSALKSAVLVEGVAKTYWLALQVGKPKILSDEDVQEHRNWYKTS
                     YGQKK"
     gene            complement(1867..3168)
                     /locus_tag="PLL88_10685"
     CDS             complement(1867..3168)
                     /locus_tag="PLL88_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HOJ02072.1"
                     /translation="MEIFSNIFNDIINPIFSQPSLILGAVTLIGLIALKRSFPDVVKG
                     TVRTIMGVLLLQQGSGLMVSMFRPIIFAFTERFQIEGVIIDPYITLSSVTDALGEKLG
                     WVGFTMLIGFGINILLVVFRKYTHLHSLFLTGHVMFLQSALITWIVYYFFGFDMAGTV
                     MTAGILIGLYWSIGAQMNAKPSQIINKGAGFTVAHQQHLMNWIAWKIGPWLGNPENSI
                     EKIKFPEWLEEFKKTEVSVPIVMFFIFAPLLAMLGRSTVQELSGLENWLLYFPIVAIS
                     FGAYMLIMNLGIGMFVNELSEAFKGISKKILPDALIGVDGMALAPYAPNAVIAGFLFC
                     ALGQLTSVLILILTKSPVLIIPGFIPVFFDAAITAVFADTFGGWKALVVISFVLGFIH
                     IFGSLWAAGNSGLVGGWMGNSDWATVWPLIMEGMKIISPVR"
     gene            complement(3176..4015)
                     /locus_tag="PLL88_10690"
     CDS             complement(3176..4015)
                     /locus_tag="PLL88_10690"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: protein motif:HMM:NF009688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HOJ02073.1"
                     /translation="MKPLIGIYEKALPLHLEWSAMLRLTAELGFDFLEMSVDESDERL
                     ARLDWSASQRKDLRRAIEEAQTPILDLCLSAHRKFALGSADEGVRAKALDIFHRAIDF
                     AAEIGIRIIQLAGYYVYYEPEDEHSLARYRAGLEVGLAHAERTGVMLALENVDGVHVN
                     SISRAMRFVNDFNSPWFQLYPDIGNLSEQGLNVSDELEAGKGHIVAVHVKDVVKGQVR
                     RIPFGKGIVDFVAAFQKLKSMAFKGPVLIEMWNDDAADSTGIVSESLRFVKEKMRLGG
                     YSE"
     gene            complement(4037..4693)
                     /locus_tag="PLL88_10695"
     CDS             complement(4037..4693)
                     /locus_tag="PLL88_10695"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HOJ02074.1"
                     /translation="MVSSRPMLQVAMDTLDLDSALKVTRQVYDVVDIIEVGTILCLAE
                     GLGAVKTIKTLYPDNLVLADVRVAEAGALISKMVFDAGANWISVLSSAALPTIETVAK
                     EAFSKGGDVQIELQEGWTMEKINQCKALGITQVIFHKSRDSEKLGPGWDPALLSVIKS
                     MSDQNFKVSVTGNLNPGDLAVFKGIPVYSFVAGRAIREASDPRAAAEQFNAAIRENWN
                     "
     gene            complement(4719..5183)
                     /locus_tag="PLL88_10700"
     CDS             complement(4719..5183)
                     /locus_tag="PLL88_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOJ02075.1"
                     /translation="MMRDLRDMLTEKTVLLNTTATDWKDAVRKGGKMLVDVKACKPEY
                     VEAMIRFAEELGPYIVIGKGLALPHARPEEGVLETCFSLLTLAEPIEFGNEYNDPVYV
                     IFCMAARDKDEHIEALRQIANLCGDEVYFEKIKNAKTLKDIQDLLEKAGDLK"
     gene            complement(5327..>5570)
                     /locus_tag="PLL88_10705"
     CDS             complement(5327..>5570)
                     /locus_tag="PLL88_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02076.1"
                     /translation="EPVLDELGLGDASVECTSIGQAQGMMSFVDLIVIAAHLRENVDP
                     PKNKPVIYVNNLVDKKEVKEKVVAALKQYYPELLKK"
BASE COUNT         1486 a         1476 c         1158 g         1450 t
ORIGIN      
        1 ggaatttttg agggtgtccc tccttgcggg gtttcgctgc taccagccgt cgccgttgtg
       61 actagggtgg attctttgga agaactttca aacaaattaa aaacagggaa tgcttcacat
      121 gcagcacagg aaaacagcag aacaatcgtt aagaaagcca atttcatatt tctaaacttc
      181 atgtcctctc ctcaaacgtg atcaatgcca gattttttcg aagtgaaaac gcgctaacac
      241 tatttttaaa ttatccattg attaatcaag ggagaacaat attatattct tgaataagga
      301 gatgtgttat catcattccc gtccaaccaa tctgccatcc attattttcc cggcagaata
      361 cattccggag catcagccgc aggattattc accaacagac tgacagggta agccatcatt
      421 tcgctttccg ggaatggttg caacagcgac accaactgag cagcaggggt ctggctgagc
      481 cattcccagg catgggtgga attgaaaatg acaggcatgc ggctatggat ggcatgcatc
      541 aatgcattgg gggcacaggt gatgatggca caggttggca ccggtttctg atcagatgtc
      601 aatcccgtag cccacaatcc tgcaaaagca aagggtttcc cacttctcaa acgaaaatag
      661 taaggggttt tgggcgctcc ttttttctcc gccggcttcc attcataaaa accatctgcg
      721 aaaatcaaac agcgttgtcc ctgctgcatc aatgtgcgga aagttacctt ttccatgatg
      781 gtctcagcgc gcacattgat caaacggata tgggctttat cgtccttcgc ccacgcaggc
      841 agcaaccccc actgcatgaa ttccattttt cgttcccatg gatcccttac cgcaggaatg
      901 aattgaccgg gggcgatgtt atagtgggca ttccaatcat gttcgattga ttcatccatt
      961 tgcaggttga attccagttg gtatgccagc gcacccgcaa cgatcgtaaa ccgtccgcac
     1021 atgatcacat ccttatcttc gccaaaagaa tttcaccact atggcaacct tctcataagc
     1081 ttacacccat taaccaagaa cgccaccttt ttttatgaag agacaatgac acgaatcata
     1141 aaaaaagtat tccatgaaga gaattgctca tggaatactt tttcattcaa tcgagtcggg
     1201 atcagctatt tcttctgacc gtagctggtc ttataccagt tgcggtgttc ctgcacatct
     1261 tcatcggaga ggatcttggg ttttcctacc tgcagcgcca accagtaagt cttggcaacg
     1321 ccctccacca gaaccgcaga tttgagcgca ctgtgggcgg acgagccgat ggtgaacacg
     1381 ccgtgatttt ccatgatgat ggcagtggaa tccccgattg aatgcaccac ttctgcaccg
     1441 atctcttctc ctccgatcgg cgcataggcg ccaatgggca cctcttttcc gaaattctcg
     1501 gcgatcgcgg taatgcaggc agggataggt ttccccaccg cagcgaaagc ggtagcatag
     1561 taagaatggg tatgcaccac gccattcacg tcagaacgat ggcggtaaat atacaaatgt
     1621 gaggcagtat ccgaagatgg ttccaatttc ccttccacca ctttaccgtc caagtcgacc
     1681 accaccatat cctcaggctt catttcctcg tacatcacac cgctgggttt gatcacgatc
     1741 agatttgatt tcaaatcccg accgctcacg tttccactcg tccaatatac caagccattc
     1801 ttgggtaatt ccatgttgca cttgtagatt tcttttttta attgttctaa catcttttct
     1861 acctttctac cgtacaggag aaattatttt catcccttcc ataatcaatg gccacaccgt
     1921 tgcccaatcg ctgtttccca tccagccccc caccagccct gaatttccag cagcccataa
     1981 gcttccgaaa atatggataa aacccaaaac gaatgaaatg accaccagcg ctttccatcc
     2041 gccaaacgtg tcggcaaaca ccgctgtgat ggctgcatca aagaaaacgg ggataaaacc
     2101 cgggatgatc agaacaggag acttggtgag tatcaggatc agaacgctgg tgagctgccc
     2161 caaagcacaa aataagaatc ccgcaatgac cgcgttgggt gcgtatggcg ccagcgccat
     2221 gccatccaca ccgatcagcg catctggaag gatctttttg gaaatacctt tgaaagcttc
     2281 gctcaattcg ttcacgaaca tgccgatgcc gagattcata atcagcatat acgccccaaa
     2341 actgatcgca acgattggaa aatagagcag ccagttttcc aaaccggata attcctgcac
     2401 ggtcgagcga cccagcatag ccaggagagg agcaaaaatg aaaaacatta cgatcggcac
     2461 gctcacttcg gttttcttga attcttccag ccactccgga aatttgatct tttcaatgga
     2521 attttccgga ttccccaacc atggaccgat cttccaggcg atccaattca tcaggtgttg
     2581 ttgatgagcc acggtaaatc ctgccccttt gttgataatc tggctcggtt tggcattcat
     2641 ctgagcgcca atgctccagt aaagccctat caagatccca gccgtcataa ccgtgccagc
     2701 catatcaaaa ccgaaaaagt aatacacgat ccaggtgatc agcgctgatt gcaaaaacat
     2761 gacatgcccg gtaaggaaca acgaatgcaa atgggtatat ttgcgaaata cgaccagcaa
     2821 gatattgata ccaaaaccga tcaacatggt aaagcccacc caccccagtt tttcgcccag
     2881 cgcatcagtc accgaagaaa gggtaatata ggggtcaatg atcacgccct cgatctgaaa
     2941 acgttcggtg aaagcaaaaa tgatcgggcg gaacatggaa accatcagcc ctgatccctg
     3001 ctgcagaaga aggacgccca tgatggttct gaccgtccct ttgaccacat cagggaacga
     3061 tcttttcaaa gcgatcaatc caattaacgt cacagccccc agaattaaag agggctgtga
     3121 aaaaattgga ttgataatgt cgttgaagat gttcgagaat atttccattg tttctctact
     3181 cgctatagcc gcctaaacgc attttctctt taacaaagcg caaactttct gatacgatgc
     3241 cggtcgaatc agcggcatcg tcgttccaca tctcaatcaa gacaggacct ttgaaagcca
     3301 tgctcttcaa tttttggaag gcagccacaa aatccacaat cccttttcca aagggaatcc
     3361 gcctcacctg tcctttgacc acatctttta catgaacagc gacaatatgc cctttgcctg
     3421 cctccaactc gtcacttaca ttcaacccct gttccgatag attgccaata tcaggataca
     3481 gttgaaacca gggagaattg aaatcattga caaaacgcat agcccggctg atggagttga
     3541 catggacgcc atccacattt tcaagcgcca gcatcacacc agttctttct gcatgagcca
     3601 gacccacttc taagcctgcg cgataccgtg caagggaatg ctcatcttct ggttcgtagt
     3661 agacataata accggccagt tggatgatgc gaattccgat ttcagccgca aagtctatgg
     3721 ctctatgaaa gatgtccagc gccttggcac gaacaccctc atccgcacta cccaaagcaa
     3781 attttctatg agcgctcaag cataggtcga gtatgggggt ttgagcctct tcaatcgccc
     3841 ttcgcaggtc ctttcgttgc gatgcgctcc aatccagacg cgccaggcgc tcatcgcttt
     3901 cgtcaacaga catctcgaga aagtcaaacc ccagttccgc ggtcaagcga agcatggctg
     3961 accattccag atgcagcggg agcgccttct cataaattcc aattagcggt ttcacggatt
     4021 gatttttctt tcggttctaa ttccagtttt cgcgaatcgc agcgttgaat tgctctgctg
     4081 ccgcgcgcgg atcactcgcc tcgcggatcg ctctaccagc cacaaaagaa taaacaggaa
     4141 ttcctttgaa cactgcaaga tctccagggt tcaaattgcc agtcaccgat actttgaagt
     4201 tttgatcact catagacttg atcaccgaca gcaatgcggg gtcccagcct ggtcctaatt
     4261 tttccgaatc gcggctcttg tggaaaatga cctgggtaat tccgagagct ttgcattgat
     4321 tgatcttttc catcgtccag ccttcttgaa gttcaatctg gacgtcgccg cctttcgaaa
     4381 aagcttcttt tgccaccgtt tcaatggtcg gcagtgcagc cgaactgaga accgaaatcc
     4441 aattcgcacc ggcatcgaag accatcttcg agatcagtgc gcccgcttca gctacccgca
     4501 catccgctaa aaccagattg tctgggtaca gggtcttgat cgtttttacg gctcccaacc
     4561 cttcagctaa acaaaggatc gttcccactt cgataatatc caccacatca tagacctggc
     4621 gggttacctt taatgcgctg tccaaatcca gcgtatccat cgccacctgg agcatagggc
     4681 gtgaagaaac catgaaatcc tcctgtgatt gtattgaatt atttcagatc acctgctttt
     4741 tcaagcagat cttgaatgtc ttttagtgtt ttggcgtttt tgatcttttc aaaatagacc
     4801 tcatcgccgc ataagtttgc aatctgacgc agcgcttcaa tgtgctcatc tttatcgcgg
     4861 gctgccatgc agaaaatcac gtacaccgga tcgttgtatt cattgccaaa ctcgatcggt
     4921 tcagccaggg tgagcagact gaaacaggtc tcaagcaccc cttcttctgg acgtgcgtga
     4981 ggaagtgcca atccctttcc gatgacgata taaggtccta attcctcagc aaaacggatc
     5041 atcgcttcga cgtattccgg tttgcacgcc tttacatcca ccaacatttt gccccctttt
     5101 cgtacagcgt ctttccagtc ggttgcagtg gtgttcagca atactgtttt ctcagtaagc
     5161 atatctctca aatcgcgcat catcacctca aactctgttc acattttcaa ttactaataa
     5221 tttgacagat gaatacacat cctctgcaca agatgatgta ttcactttat tttcaactgc
     5281 tctgctagat gaaaagttat ggtatgccag tagggcttga aatattttat tttttcaaaa
     5341 gttctggata atattgctta agcgccgcga ctactttctc tttgacttct tttttatcga
     5401 ccaggttgtt cacataaatg accggtttat tcttgggtgg gtcaacgttt tctctaaggt
     5461 gagcagcaat gacaatgagg tcaacaaaag acatcatccc ttgtgcctga ccaatggaag
     5521 tgcattctac cgaagcgtca cccaatccca attcgtccaa aactggttcc
//
LOCUS       DAOSDS010000129         5499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5298_length_5499_cov_4.590926, whole genome shotgun sequence.
ACCESSION   DAOSDS010000129 DAOSDS010000000
VERSION     DAOSDS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5298_length_5499_cov_4.590926"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(253..753)
                     /locus_tag="PLL88_10710"
     CDS             complement(253..753)
                     /locus_tag="PLL88_10710"
                     /inference="COORDINATES: protein motif:HMM:NF025255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4203 domain-containing protein"
                     /protein_id="HOJ02077.1"
                     /translation="MNWVYLALGVAILILGRKFFWLFTGGIGFYIGYTLAPKILPNQS
                     ENVILIVAVVLGLLGVFLAMLVKSAAIGIAGFAAGAYVVYSLIPMINFDLGNYYWLVV
                     IVGGVLGAILAGTMFDWALIILTAACGAMLISTTLNLSFPLSAVVLVVLFLIGLIVQG
                     NMKSKD"
     gene            complement(849..1553)
                     /locus_tag="PLL88_10715"
     CDS             complement(849..1553)
                     /locus_tag="PLL88_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02078.1"
                     /translation="MAAKKEETVDGKPLEAVEQTAASDKEKAKEIKAKVESSEKAMKT
                     EIAKDEMPGANAPLSFFHGPVLLGIALLFLGIVLLGGELLGFRFGTILWPFIFIIPGA
                     VLFLSALSSQDSHAEGVAILGSMMAALGVIFLLQQMLNMWASWAYAWALLAPTSIGVA
                     QVIFGKQHNRDSLVKNGKGLIEVGLTLFFIFFVFFELILNISGKNFIPFGLPAFPVAL
                     IVLGIFVIVRSVLRKK"
     gene            complement(1632..2531)
                     /locus_tag="PLL88_10720"
     CDS             complement(1632..2531)
                     /locus_tag="PLL88_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOJ02079.1"
                     /translation="MHTHGHEHRYNHSSNSTLGDIKLAFFLNVIFSVAEFIGGLLINS
                     VAVLSDSLHDFGDSISLGLSWYFERLSHRNRNGKYSYGFRRFSLLGAVINAIILIVGS
                     VFIIREAVLRLKEPAQTDVRGMLIFAIIGIVVNGFAVYRMQRGKTMNAKMLTWHLLED
                     VLGWVAVLIVSIVMLFVDIPALDPILSLGLSAFVLYNVIKTLIETISLFLQAVPADVD
                     MDEIERRMKRLDKVEDVHHTHVWSLDGEENVLTTHIVLTEGAKSSDIRRIKESIRQIA
                     REFHCQHTTVEFEYLKEDCSIPC"
     gene            2618..3526
                     /locus_tag="PLL88_10725"
     CDS             2618..3526
                     /locus_tag="PLL88_10725"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOJ02080.1"
                     /translation="MKVGYIGLGIMGKPIARNILKGEFPLVVHNRSQEAVEELVGEGA
                     TRASTPQEVAEQVDVVFTNLPDSPDVEKVVLQERGILAGAHPGLIHVDNSTIKPLVAR
                     KLAAMLAEKGVRSLDAPVSGGDIGAINGTLSIMVGGSQEALEKVRPVLNAIGKTITHV
                     GSNGAGQIAKAANQIMVAAQMVAMAELLIFTQKAGADPQKVIQAIQKGAAQCWTLDTK
                     PQRLFAGNRDPGFKAALQAKDLNIIMESAHEFGVPLPMTAVTDQLYQAMLQNGMGELD
                     NSAIVGIIEKLANTQLVSHTHIPQEE"
     gene            3528..4247
                     /locus_tag="PLL88_10730"
     CDS             3528..4247
                     /locus_tag="PLL88_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ02081.1"
                     /translation="MDHFTGVYKNQPREYHQMIEVEDFENNLLKAILRFTPISSNRVL
                     DIGSGTGRLPLLFHAQNADLLALDISFPMLLEQRAQKQNIGGKWELIQGDMRILPVRD
                     SWADVTTAGWAAGHFCGWFPETWQQEIDRFILEMQRVTRPGGALFIMETLGTGRKTPA
                     PPTRALHEYYQRIESQWGFQREVIRTDYKFQSVSDAIEKTAFFFGQELAETIDKNEWQ
                     IVPEWTGVWHRVNKVFISFNL"
     gene            complement(4237..5025)
                     /locus_tag="PLL88_10735"
     CDS             complement(4237..5025)
                     /locus_tag="PLL88_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOJ02082.1"
                     /translation="MTKLFQVDAFTDRVFSGNPAAVCLLEDEKADTWMQALATEMNLS
                     ETAFLTKQEDGYGLRWFTPATEVKLCGHATLASAWVLFTEKIVSTDQVIHFHTLSGLL
                     AARWENGSVALDFPAFRGAKSIEKPALMKALGVKQGALWQAGPHQFVELESAETLRRL
                     APDFDRMVALGNGDLAVTCRSDDPAYDFISRFFAPALGVNEDPVTGSAHCSLGPFWME
                     RLGKDRLRAFQASTRGGSLLVNVLGDRVELHGKAVTVFQAELKD"
     gene            5137..>5499
                     /locus_tag="PLL88_10740"
     CDS             5137..>5499
                     /locus_tag="PLL88_10740"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOJ02083.1"
                     /translation="MKEYMQRVLEKDVSESEKMRRLGENRAFHPLAVFLAHSGDSWYW
                     LAFLFLVWLFSRSSWHTYSAFLAIAILIQAVLVLAIKFIIKRSRPAGDWGAIYRNSDP
                     HSFPSGHATRAAMLAVLFW"
BASE COUNT         1549 a         1395 c         1223 g         1332 t
ORIGIN      
        1 ctcaccaaca gtgacacatg ggagccccat gctgagcgtt aaggtatcca tggtccgtac
       61 caccagcagc agcaaagcgc tgcattacaa ctgccggtgt cacgtcattt cgtcgaattc
      121 agacagggca cttccatgtt aatttccgca acctctgcga acccgcaatt ttacagcccg
      181 tgcgcgcgac ctggatccag ccgacctttt catccagcat ccaacgcatc agcatcacaa
      241 ccggcgcatc gatcaatctt ttgatttcat attgccttgc acgatcagac cgatcagaaa
      301 gagaacaacc aacactacag cagataaagg gaaagataag ttcaaggtag ttgaaatcag
      361 cattgcccca caagcagcgg tcaggatgat cagagcccaa tcaaacattg ttccagccaa
      421 gattgcccct aacacgccac ccacaatgac caccagccag taataatttc ccagatcaaa
      481 attgatcata gggatcaagg aatatacaac atacgcccct gcagcaaacc cggctatgcc
      541 gatggcagcg ctcttgacca acatcgccag aaaaacgcct aataagccca gcacaactgc
      601 aacgatcaaa atgacatttt cagattgatt ggggagaatt ttaggagcca gcgtataccc
      661 tatgtagaaa ccaattccac cggtaaacag ccagaaaaac tttctaccca agatcaatat
      721 cgcaacgcca agagcaagat acacccaatt catatttgaa ctcccaacgc caaatttcta
      781 tgaatcaatt atattcttat gccatgagga aacaaagaaa aaaagtgctc tttcaagcac
      841 ttttttcgtt attttttgcg caggaccgag cgcacgatca cgaaaatacc cagcacaatc
      901 aacgctaccg ggaaagcggg caaaccgaag ggaatgaaat tcttcccgct gatattcaag
      961 atcaattcga agaatacgaa gaaaatgaag aacagggtta atcccacttc gatcagtcct
     1021 tttccattct tcaccaggga gtcgcgattg tgctgctttc caaaaatcac ctgagccacc
     1081 ccaatggagg tcggcgcaag caatgcccat gcgtaagccc agcttgccca catattcagc
     1141 atctgttgca agagaaaaat gactcccaga gccgccatca tcgaacccaa gatcgctacc
     1201 ccctcggcat gggaatcttg ggaagaaagt gcgctcaaaa acaacacagc gcctgggatg
     1261 ataaaaatga acggccacaa aatggttcca aaacgaaaac caagcaactc gccccccagc
     1321 aaaacaatgc ccaaaaagag caaagcaatt cctagaagga ctggaccatg gaaaaatgaa
     1381 aggggagcat ttgcccccgg catttcatct tttgctattt cagttttcat agctttttcc
     1441 gacgattcga cttttgcttt aatttctttt gccttttctt tatcagaagc tgctgtctgt
     1501 tctaccgcct cgagcggttt tccatcaact gtttcttctt tttttgctgc cattacacac
     1561 catcctgtat ctcttcattc ttttgtagaa tatacgcaaa accatagaat tggttgcgga
     1621 ttttcgatag ctcaacacgg tatggaacaa tcttccttca agtattcaaa ttcaacggta
     1681 gtatgttggc aatgaaattc ccgcgctatc tgacggatcg attccttgat cctacggata
     1741 tccgaacttt tggcgccttc cgtcaaaacg atatgcgtgg tcagaacatt ttcttctcca
     1801 tccagagacc agacatgggt atgatgcaca tcttccacct tatccagccg tttcatccgc
     1861 cgttcaattt cgtccatatc cacatctgca ggaaccgctt gcagaaagag agaaatggtt
     1921 tctattaaag ttttgatcac attgtagagc acaaacgcag acaaccccag cgaaaggata
     1981 ggatcgagcg caggtatatc aacgaataac atcacgatgc tcacgatcag cacggcaacc
     2041 cagcctaaca cgtcctccag caggtgccag gtcagcattt tggcgttcat ggtcttccca
     2101 cgctgcatcc gatatacggc aaacccgttg accacaatac caatgatcgc aaagatcagc
     2161 atgccgcgca catcggtttg agcgggttct ttcaatcgca gaaccgcttc tcgaatgatg
     2221 aacacggatc caacgatcag aatgatcgca ttgatcaccg caccgagcaa agaaaatctt
     2281 ctaaatccat aagaatactt tccatttcga ttacgatgcg ataacctttc aaaataccat
     2341 gaaagaccta aggaaatgct atctccaaaa tcatgcaagg aatctgaaag aaccgctacc
     2401 gaattgataa gcaacccgcc gataaattca gcgaccgaaa aaatgacatt caggaaaaat
     2461 gctaatttaa tatctccaag cgtgctgttg gaagaatgat tatacctatg ctcatgcccg
     2521 tgtgtatgca ttttaagcgt ccttcaattt ataatggtta tcaaattcta ctccatgaaa
     2581 caagtttctg tgagaatcct ttggtgagga gagagatatg aaagtaggtt acattgggtt
     2641 aggcatcatg ggaaaaccca tagcgagaaa tattcttaaa ggtgagtttc ccctggttgt
     2701 tcacaaccgc agtcaggaag ctgttgaaga gcttgtgggc gaaggcgcca ctcgtgcttc
     2761 gaccccgcag gaagtcgccg aacaggtcga tgtggttttt accaacctgc ccgattcgcc
     2821 ggacgttgaa aaagttgtac tgcaggaacg tggcatactg gcaggggcac acccgggctt
     2881 gatccacgtg gacaactcaa ccataaaacc tctggtggca cggaaattgg ctgccatgtt
     2941 ggcggaaaaa ggtgttcgaa gtttggacgc gccggtcagc ggtggcgata ttggcgccat
     3001 taatggaacc ctgtccatca tggtcggtgg gtcacaggaa gctctggaaa aagtacgacc
     3061 tgttctcaat gcgattggaa agaccatcac tcatgttggt agtaatggcg ctggtcaaat
     3121 tgcgaaggca gccaaccaga tcatggtagc tgcgcaaatg gtagccatgg cagagctgtt
     3181 gatcttcaca cagaaagcgg gggcagaccc ccaaaaagtt atccaggcaa ttcaaaaggg
     3241 agccgcccaa tgttggacgc tcgataccaa accgcaacgg ctatttgccg gtaatcgaga
     3301 ccccggattt aaagcggctt tgcaagccaa agatctcaac atcattatgg aatcagccca
     3361 cgagtttgga gtgcctctcc ccatgaccgc tgtcaccgac cagctttatc aagccatgct
     3421 acagaatggg atgggagagc tagacaattc ggcaatcgta ggtatcattg aaaaactggc
     3481 aaatacgcaa ctggtatcgc atacacacat accacaggag gaatgacatg gaccatttta
     3541 ccggggtcta caaaaatcag ccccgtgaat atcatcaaat gatcgaggtg gaagatttcg
     3601 agaataatct tcttaaggct atcctgcggt tcactcctat ctcttcaaat agagttctag
     3661 acattggcag cggaacaggt cgtttaccct tgctttttca cgctcaaaat gcggatttgt
     3721 tggctttgga tatcagcttt cctatgttac tggaacaaag agcgcagaag cagaacattg
     3781 gagggaaatg ggagcttatc caaggagata tgcgcatact gccggttcgt gacagttggg
     3841 cagatgtgac cactgctggt tgggcagccg ggcatttttg cggttggttc cctgaaacct
     3901 ggcaacaaga aatagaccgt ttcatcctgg aaatgcagcg cgttaccaga ccaggtggtg
     3961 cgttatttat catggagact ttgggtaccg gcagaaaaac tcccgctcct cctactcgcg
     4021 ctctacatga gtattaccag cggattgaat ctcaatgggg tttccaacgt gaagtgatca
     4081 gaactgatta caaatttcaa tcagtgagtg acgcaataga aaaaaccgca ttcttctttg
     4141 gacaggagct cgcagaaacc atcgacaaaa atgaatggca gatcgttccc gaatggaccg
     4201 gggtatggca tcgggtgaat aaagttttta tatctttcaa tctttaagtt ctgcttgaaa
     4261 gacggtcact gcttttccat gtagttccac acggtctccc aatacattca ccagcaggct
     4321 tccaccgcga gttgaagcct ggaaagcccg caggcgatcc tttcccaatc gctccatcca
     4381 aaatggaccc aaactgcaat gagccgatcc ggtgaccgga tcttcattca ctccaagagc
     4441 cggggcgaaa aaacgggaaa tgaaatcata agcgggatca tccgaccggc acgtcactgc
     4501 cagatcgccg ttgccaaggg caaccatccg gtcgaaatct ggcgccagtc ttctaagagt
     4561 ttcagcgctc tccaattcaa caaattggtg cggacctgcc tgccagagtg caccctgttt
     4621 cactcccaga gctttcatca gagccggttt ttcaatgctc tttgctcccc ggaaggcggg
     4681 aaaatccaat gccactgaac cattttccca tcgtgcagcc aacaatccgc ttagcgtgtg
     4741 aaagtggatc acctggtcgg ttgatacgat cttttctgtg aaaagtaccc aagcgctcgc
     4801 cagtgtggca tgcccgcata atttcacttc ggttgcaggg gtaaaccagc gcaatccata
     4861 tccatcttcc tgttttgtca aaaaggcggt ttccgagagg ttcatctctg ttgccaatgc
     4921 ctgcatccag gtatcggctt tttcatcttc cagcagacaa accgcagccg gattgccgct
     4981 aaaaacgcgg tcggtaaaag catccacttg aaatagtttg gtcatgatcc actccagaat
     5041 attgcctgac aatattgtaa acgaaaaact ccatccgccc tctgcggatg attaatgtta
     5101 aaatgggttc catcttgtgg aagggaatct gaccgtatga aagagtacat gcaaagggtt
     5161 cttgaaaagg atgtgtctga atcagagaag atgcgcagac taggggaaaa tagagcgttc
     5221 catcctctgg cggttttcct ggcacattct ggagattcct ggtactggtt ggcatttctt
     5281 tttctcgtct ggttattttc ccgatctagc tggcatacct attccgcatt tttggcgatt
     5341 gctattttga ttcaagccgt tctggtgctg gcgattaaat tcatcatcaa gcgcagccgc
     5401 ccggcaggtg attggggcgc catttatcgc aacagcgatc ctcattcttt tccttccggg
     5461 catgccaccc gggctgccat gttggcggtg ttgttctgg
//
LOCUS       DAOSDS010000130         5451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5356_length_5451_cov_4.356004, whole genome shotgun sequence.
ACCESSION   DAOSDS010000130 DAOSDS010000000
VERSION     DAOSDS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5451
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5356_length_5451_cov_4.356004"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLL88_10745"
     CDS             <1..210
                     /locus_tag="PLL88_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02084.1"
                     /translation="KGGKNNGYFIVISLTATQDMQIPGQSISFQQLLLAQTLGDMDAL
                     EAAGRKVLYLHLKNADLATIIKHLH"
     gene            complement(207..1085)
                     /locus_tag="PLL88_10750"
     CDS             complement(207..1085)
                     /locus_tag="PLL88_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOJ02085.1"
                     /translation="MYVGIEAGGTKFNCIIAQHPGQVLGEKRITTGEPEKTVQEVVKF
                     IRQTVDQDAHRLKAIGIGSFGPLDLNPASPDFGSITSTPKKGWGSFNIKKAFEDALEI
                     PAVIDTDVNAAALGEFLWGAGQDRAHVLYLTIGTGIGGGIVVNGKPYHGMLHPEMGHI
                     LLPHEAHFKGICPYHGHCFEGLASGPALQARWGVEASRLEKGHPAWEEEARVIALALC
                     NYICILSPDIIILGGGVMQQSHLFPLIRSHVREFLNGYVQADPIMKDINTFIVPPGLG
                     TRSGMLGALALARQVT"
     gene            1213..2391
                     /locus_tag="PLL88_10755"
     CDS             1213..2391
                     /locus_tag="PLL88_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOJ02086.1"
                     /translation="MDIFKKCKEYTDAKEVMEKGIYPYFLPLAENEGAEVVFNGKRMI
                     MCGSNNYLGLTTHPKVKEAAAKALERFGTSCTGSRFLNGTLELHEQVEKEIADFVGKE
                     EALIFSTGMQTNLGTISALVSRDDVVILDKEDHASIVDGARLGYGKIERFSHNDIEHL
                     ERVLKSIPDERGRLIVVDGVFSMGGDLADLPRIIPMAQKYGARVMVDDAHGMGVVGGG
                     RGTAHAFAMDDHVDLIMSTFSKSFASLGGFIAGDSDIIHYIKHTARSLIFSASIPASN
                     AAAVLAAIQVIREEPERVERVNAIGETIRRELRAMGFNIGESVTPIVPVIVGDDELTF
                     RSWKALFENGVFVNPVISPAVSAGQQLLRTSYMATHTDEHIEKVLQVFKKVGKELAII
                     "
     gene            complement(2588..2929)
                     /locus_tag="PLL88_10760"
     CDS             complement(2588..2929)
                     /locus_tag="PLL88_10760"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOJ02087.1"
                     /translation="MSEETSLPGWKASTSHPELVEKIRKALRQVRDPEIGNDIIELGL
                     VRDVEIAEKNVVVTMILTTPYCPYGPALMESARRVVEKESEKSTTIIYGNEIWDQSMM
                     EDSISFNWGLF"
     gene            complement(2926..3555)
                     /locus_tag="PLL88_10765"
     CDS             complement(2926..3555)
                     /locus_tag="PLL88_10765"
                     /inference="COORDINATES: protein motif:HMM:NF016581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ02088.1"
                     /translation="MNAMKTTREKVLTTIKLHPKSTIMEIADQVGINAISVRHHLTNL
                     QAEGLIMAEEERHGVGRPRLVYALTEQGMETFPSRYFKLVNSLIDQLKETLPEKEVSS
                     LFYNMAEKLTADYEPMLAKMSIEERLELLKTILNDEGFEMDWEQEGKTCQIRELSCPY
                     FKIGDYHPEVCVFGKSVISNILHVPLNKISHQEREKDLCTYTVDLSSKE"
     gene            3683..4435
                     /gene="sufC"
                     /locus_tag="PLL88_10770"
     CDS             3683..4435
                     /gene="sufC"
                     /locus_tag="PLL88_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOJ02089.1"
                     /translation="MIGLEITDLHVQVEDVEILHGINLLVPAGEVHAIMGPNGTGKST
                     LAYALMGHPKYQVSAGDIFLNGKSILSLSPEERSHAGLFLAFQYPVAIPGVTVANFLR
                     TAINAHRKATDPTDHGLLIPEFRKLMLEKMKLLEMDPKMGGRYLNDGFSGGEKKRVEI
                     LQMAMLEPRMAVLDETDSGLDIDALRIVAEGINTLKNDQMGILLITHYQRVLNYLKPD
                     VVHVMVDGKIVESGGPDLALRLEEEGYRWVKA"
     gene            4467..>5451
                     /gene="sufB"
                     /locus_tag="PLL88_10775"
     CDS             4467..>5451
                     /gene="sufB"
                     /locus_tag="PLL88_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOJ02090.1"
                     /translation="MPDQEEVLRGIDTQRYDFKDPENYVFKSRKGLDAEVVEQISAYK
                     EEPQWMRENRLKALRQFNSMPIPGWGADLQGLDLDDLYYYVKPTDEIQNDWDELPDTI
                     KNTFNRLGIPEAEQKFLAGVGAQYESEMVYHNIQKQLESQGVIFLSIEDGLRQHPDLF
                     KEHFGTVVPFNDNKFAALNTAVWSGGSFVYIPPFVKVELPLQAYFRLNTASIGQFERS
                     IIIADEGSQVHYVEGCTAPQYSKNSFHSGVIEIIVKKGARVRYTTIQNWSTNVYNLVT
                     QRARVHEEGTMEWVDSNLGSKVTMKYPACYLVGPHAHGEILSMAFAGRNQVQ"
BASE COUNT         1445 a         1262 c         1389 g         1355 t
ORIGIN      
        1 aaaggtggca agaacaacgg ttatttcatt gtgatcagct taactgccac acaggatatg
       61 caaattccgg gacaatcgat ttccttccaa caactgttgc tggctcaaac cctgggcgat
      121 atggatgcat tggaagctgc gggaagaaaa gtgctgtatc tgcacttaaa aaacgcggac
      181 ttagctacta ttatcaagca tctgcattag gtcacctgac gagccagcgc aagcgcaccc
      241 agcatgcccg aacgggtccc taacccaggg ggcacgatga aggtgttgat atccttcatg
      301 atagggtctg cctgcacata tccattcaag aattcacgga cgtgagaacg aataagggga
      361 aagaggtgag actgctgcat cactcccccg ccgagaatga tgatgtcggg tgaaaggatg
      421 cagatgtaat tgcaaagcgc caaagcgatg acgcgggctt cttcttccca ggcaggatgc
      481 cctttttcaa gccggctggc ttccacaccc cagcgtgcct gtagggcggg accactggct
      541 aacccctcga agcagtgccc gtgatatggg cagataccct tgaaatgcgc ttcatggggc
      601 aacagaatat gccccatttc aggatgcagc atgccgtgat agggcttccc attcaccact
      661 ataccacccc caatgccggt gccaatggtc aggtaaagaa catgcgccct atcctgaccg
      721 gcaccccaca aaaattcgcc caaagcagcc gcattgacat cggtatcgat cacggctggt
      781 atttccaaag catcttcgaa cgcctttttg atgttgaagg acccccatcc ttttttcggg
      841 gtggatgtga tcgaaccgaa atcaggcgaa gcggggttga gatccaacgg tccgaaggaa
      901 ccgataccga tggctttcaa tcgatgcgca tcctgatcca ctgtttgccg gatgaatttc
      961 accacttctt gcaccgtttt ttcaggctca ccggtggtga tccttttttc acccagaacc
     1021 tgtccaggat gctgtgcgat gatacaattg aactttgttc caccagcctc aattccaacg
     1081 tacatgcctt actccctgtt gtgatgatac tttaatttta gcttgaaaat attccatccg
     1141 accgaaaatc acaacgcttg tataattcta gggttaaagc atgtgtattc atcatgaaag
     1201 gatttatcac gcatggacat ctttaagaaa tgcaaagagt ataccgatgc caaggaagtc
     1261 atggagaagg gcatttaccc ttatttctta cccctggcgg aaaatgaagg cgcagaggtc
     1321 gttttcaatg gcaaacgcat gatcatgtgc ggttcgaaca attacctggg attgacaacc
     1381 caccctaaag tgaaagaggc ggctgcaaag gcgcttgagc gttttggaac aagttgcacc
     1441 ggttctcgct ttttgaatgg aacgcttgaa ctgcatgaac aggtcgaaaa agagatcgca
     1501 gattttgttg gaaaagaaga agcgttgatc ttttctactg gtatgcagac caacctgggc
     1561 accatcagcg cgctggtgag ccgtgatgat gtggttattt tggacaaaga agatcatgcc
     1621 agcattgtgg atggagctcg attgggatat gggaagatcg aacgcttctc acacaacgat
     1681 attgaacacc tcgagcgcgt gttgaaatca attcctgacg aacgtggaag actgatcgtg
     1741 gtggatgggg tgttctccat gggtggagac ctggcggacc tgcccaggat tatccccatg
     1801 gctcaaaaat atggagcgcg tgtgatggtg gacgacgccc acgggatggg cgtggtgggg
     1861 ggaggacgcg gtacggcaca cgcgttcgcc atggatgatc acgtcgatct gatcatgtct
     1921 accttcagca aatcattcgc gtcgctgggc gggttcattg ccggcgatag cgacatcatc
     1981 cattacatca agcacacagc ccgttccctg atcttcagcg ccagtatccc ggcatccaat
     2041 gcggctgcag ttctggcagc tattcaggtg attcgtgagg agcccgaacg ggtggaacgg
     2101 gtaaacgcaa ttggagagac gatccgcaga gaactgcgcg ccatgggctt taatatcggc
     2161 gagtcggtca cacccattgt gccggtcatc gttggtgatg atgaattgac tttccgcagt
     2221 tggaaagcct tatttgaaaa tggtgttttt gtgaatccgg tcatttcacc ggctgtttct
     2281 gccgggcaac aactgctgcg caccagctat atggcaaccc ataccgatga gcatatcgag
     2341 aaagtgctgc aggtcttcaa aaaagtagga aaagagctcg cgatcatcta aacggtacat
     2401 tgtaaacagg aataaaaata agcttgagaa agatcaagct tatttttatt taggggtcat
     2461 cttaaaaagc ctgtggaaca gcgcgctatc cctcatgcgt tcgcctagcc acctttgata
     2521 aaactggtga ggaaaaggaa tgtttttcca ttactttatt ttttggaatg gcagcgagga
     2581 aaggcggtta gaaaagaccc caattgaagg agatggaatc ttccatcatt gactgatccc
     2641 agatttcatt gccataaatg atggtggtgc ttttttcgct ttccttttcc acgacgcgtc
     2701 gcgctgattc catcagcgct ggtccatacg ggcagtaggg agttgtcagg atcatcgtaa
     2761 ccaccacatt cttctcggcg atctcgacat cgcgcaccaa ccccaattca atgatatcgt
     2821 ttccgatttc cggatcgcgc acctggcgca aggcttttct gatcttttcc accaattcag
     2881 gatgggaagt gctggctttc cagccgggca gagaggtttc ttcgctcatt ctttgctcga
     2941 tagatcaacc gtgtaagtac ataaatcttt ttcacgttct tgatgtgaga tcttgtttag
     3001 tggaacgtgg aggatatttg agatgaccga tttcccaaag acgcagacct ccggatggta
     3061 atcgccaatc ttaaaatacg gacaggataa ttcgcgaatc tggcaagttt tgccttcttg
     3121 ttcccagtcc atttcaaaac cttcatcatt cagaatagtc tttaataatt cgaggcgttc
     3181 ttcgatggac attttcgcca acatcggctc ataatcggcg gtcagttttt cagccatgtt
     3241 gtagaagagg gaactcactt ccttttcagg cagtgtttct ttgagttgat ctatcaagga
     3301 attgaccaat ttgaaatagc gcgacggaaa agtttccatg ccctgctctg tcaaagcata
     3361 caccaggcgc ggtctgccca ccccatggcg ttcctcttca gccatgatca gcccttcagc
     3421 ctgcaagttg gtgaggtgat ggcgcactga gatggcatta atgccaacct gatctgcaat
     3481 ttccatgatt gtagactttg gatgcaattt aatcgtcgtc agaactttct cacgggttgt
     3541 tttcatcgcg ttcataagat tttaccacta tcactatatc acgcagacca gaaaatgtca
     3601 atatttgtga tatttataat aaatattgac taaaagcgtc ctgcgtggta tactgcacgc
     3661 tgctaccgga aaggaaagaa aaatgatcgg tttagaaatc actgacctgc atgtacaggt
     3721 tgaagacgta gagatcttac atggtatcaa cttgctggta ccggctggag aggtgcacgc
     3781 catcatggga ccgaatggaa ccggaaaatc aacgctggcg tatgccctga tgggacaccc
     3841 aaaatatcaa gttagcgcgg gggatatttt cctcaatgga aagagcattt tatccttatc
     3901 ccctgaagag cgatcccatg cagggctatt tctggctttt caatacccgg tggcgatacc
     3961 aggagtgacc gtggcgaatt ttctccgcac cgcaataaat gcgcatagga aagcgactga
     4021 cccgaccgac catgggctgc tcatcccaga attccggaag ctgatgctgg aaaaaatgaa
     4081 attgcttgaa atggatccca agatgggcgg gcgatatttg aacgatgggt tttctggtgg
     4141 agagaagaaa cgtgtggaaa ttctgcagat ggcaatgtta gaacccagga tggcagtgct
     4201 ggatgaaacg gattcgggtt tggatatcga tgctctgcgg attgtagcgg aaggaattaa
     4261 tacgctcaaa aacgatcaga tgggcatctt gctcatcacc cattaccagc gcgtgttgaa
     4321 ttacttgaaa ccggatgtcg tgcatgtcat ggtggatgga aagattgtgg aaagcggtgg
     4381 acctgacctg gcgttgcgcc tggaagaaga aggctatcgg tgggtaaaag cataatagca
     4441 ggaaagtatg agacagagag atagccatgc ctgatcaaga agaagtctta cgtggaatcg
     4501 atacccaacg ctacgatttt aaagatcctg aaaattatgt ttttaaatcg cgcaaggggc
     4561 ttgatgcaga agttgtagag cagatctctg catataaaga agaaccgcag tggatgcgag
     4621 aaaacaggtt aaaagccctg cggcaattca actccatgcc catacccggc tggggggcag
     4681 acctacaagg gttggatctg gatgatctgt attattacgt taaacctacc gatgaaatcc
     4741 aaaacgattg ggatgagcta ccggatacca tcaaaaacac gttcaaccgt ttgggaattc
     4801 ccgaagcgga acagaaattt ctggcgggtg tgggagccca gtacgaatcg gaaatggttt
     4861 accacaacat ccagaaacag cttgagagcc agggagtcat ctttctgagc attgaagacg
     4921 gattgcgcca gcatccggat ctcttcaaag agcattttgg gacagtcgtt ccatttaacg
     4981 ataacaagtt tgcagcattg aacactgctg tttggtcagg cggctctttt gtatacattc
     5041 caccgtttgt aaaggtagag ctgcctcttc aggcatattt tcgtctgaac accgccagca
     5101 taggacagtt cgagcgcagt attatcattg ccgacgaggg ttcgcaggtt cactatgttg
     5161 aaggctgcac tgcgccacag tattccaaga attcctttca cagcggagtg atcgagatca
     5221 tcgtgaaaaa gggagcgcgg gtgcgctata ccaccattca aaactggtca accaatgttt
     5281 ataacctggt aacccagcgc gccagggtgc atgaggaggg caccatggag tgggtagaca
     5341 gcaacctggg ctcgaaggtg accatgaaat accctgcctg ctatctggta gggcctcatg
     5401 cccatggcga aattctatcg atggcttttg ccgggagaaa ccaagtgcag g
//
LOCUS       DAOSDS010000131         5406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5400_length_5406_cov_4.124650, whole genome shotgun sequence.
ACCESSION   DAOSDS010000131 DAOSDS010000000
VERSION     DAOSDS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5400_length_5406_cov_4.124650"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1795)
                     /locus_tag="PLL88_10780"
     CDS             complement(218..1795)
                     /locus_tag="PLL88_10780"
                     /inference="COORDINATES: protein motif:HMM:NF017919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02091.1"
                     /translation="MLTSDQLDVLMEPIINLYQEFEERAIIDIVRRLIKMNFDSPSAA
                     WMVQRLSESGMLYEEILERISQLTGKSELELQRIFRKAGVKTLQFDDVIYKAADLQPL
                     PLNLSPAMTQVLAAGLRKTQGVVRNFTLTTASAGQELFISVADRVYLEVSSGAFDYNS
                     AMRQAVKQVASQGLTTINYASGHRDQLDVAMRRTLLTGVGQTVGNLQLARADEMGCDL
                     VAVSAHIGARNKGVGPMNHESWQGKVYSRSGTNPKYKPFIETTGYGTGPGLMGWNCRH
                     NFYPFFEGISENAYSSSELDSYERKRVTYQGQEMSVYDASQIQRGIERKIRYWKRQAG
                     GLEAAGLDNTFERLKMGEWQAEMRQFVKETGLIRQSEREQINGLGKIRALTKKTVVEQ
                     TIANIPALSNTGRKIINIGEIGENIAPIIQSRSSAKIVQATDDVLKHIEKHKDQFDIL
                     KAKNMLPEVLNNPKFLFQGKKKTSLQFIEDFDEKYFLMIPIKYLPNELWLETLTIEEK
                     ERFLRRWMKRELLYKRE"
     gene            complement(1773..2111)
                     /locus_tag="PLL88_10785"
     CDS             complement(1773..2111)
                     /locus_tag="PLL88_10785"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD3 domain-containing DNA-binding protein"
                     /protein_id="HOJ02092.1"
                     /translation="MNEKTAKRLSGVYEIVNTVNGHRYIGSSANISKRWNEHKRKISA
                     SLIGNKHTLGQTPWNKGKTGLQNHSQETRQKMSEAHKGKRLTEETRRKLSETKKKYWE
                     EKRLANERSA"
     gene            complement(2119..3531)
                     /locus_tag="PLL88_10790"
     CDS             complement(2119..3531)
                     /locus_tag="PLL88_10790"
                     /inference="COORDINATES: protein motif:HMM:NF016988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOJ02093.1"
                     /translation="MFSKIINWIKDWWTKMIGKQSIKQAVGQDIAISDPMVKALESWS
                     KMYENKSNWLNDYVKSLNLPAAIAREISRMATIEMAIALDGSKRADYLAEQFNNVMGK
                     IREVIEFGCAKGGLVFKPYPDGKNISVDYIHADQFFPISFDSSGHITACVFIDQRKQG
                     DYWYTRLESHQMTGDGYIVRNSAYRSSTKDILGGQVSLELIDDWKELEPEATIVGLDK
                     PLFAYFRYPLANNIDPKSPLGVSCYAGATDLIKQADILWSDLMWEFESGKRALYVDAS
                     AFDKTADDKPIIPDRRLYRALNQMESAQIGEPGFYEEWSPEFREVSIKSGLNTILQRI
                     EFNCGMAYGTLSDPQIVDKTATEIKTSKQRSYSTVVDVQKAIKIALDDLIYAMDTWAT
                     INALAPKGKYTVTYDFDDSVVVDKDAQLMTDRQTVSMGSMAKWRYLMRNYGLSEEEAK
                     KWVAEATDEQPSETDLFQGA"
     gene            complement(3553..5241)
                     /locus_tag="PLL88_10795"
     CDS             complement(3553..5241)
                     /locus_tag="PLL88_10795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HOJ02094.1"
                     /translation="MSLSCKQRIFIDEYLKCWNATQAALLAGYSERSAGSIGGENLKK
                     PEIAAEIQLRLAESAMSADEVIQAIGEIGRADIGDFVEIDEGTGRIKNIDFGKAQKSG
                     KLRLIRSITPTANGLKIELHDRMRALELLGKRYGLFEDRAQEQSDAESGPFYLPANAI
                     APSFYGAYRDICDHKHTEYVLKGGRGSTKSSFTSEVIIELIINNPDWHALITRQVKDT
                     LRDSVFSQLQWAINYLGLADKFRCTTNPLEITYIPTGQKIYFRGGDDPLKIKSIKPRF
                     GYINILWFEELDQFRGAEAVRSIIQSAIRGGDKAYIFKSFNPPRSRNSWVLKELEIPK
                     PDRYVHHSTYLDVPREWLGQTFIDEAEFLKEINPSAYEHEYMGVSTGVGGLVFDNVEI
                     RKITDEEITQFDHVLHGQDFGYFPDPAHYARCHYDAARLTLYIFGELRRWKTSNRDLY
                     SALVDYGLTSQDMLICDSAEPKSVADLRDYGVSARGAEKGPESVRYSMKWLQSLKAIV
                     IDNERAPYSAEEFLNYEHETDRDGNYISDYPDENNHAIDAVRYACNLIWRRRGQ"
BASE COUNT         1321 a         1368 c         1080 g         1637 t
ORIGIN      
        1 catgcctcgt caagtgagaa aaaatatatt cagttgtatc ttacacgtgc attatatcac
       61 aaaaaatata caagcaatta tcaaggactt agaatatgtt tctgattgtt ttttttatat
      121 aactatgtta ctatgagatt gcatctgagg tggaaaatca ggctccaatt acccgcaagg
      181 gattgatgcc ggttagcctg cccggccaga tgctttttta ttcccgctta tacaataatt
      241 ctctcttcat ccatctacga aggaaacgtt ctttttcttc aattgtcaaa gtttcaagcc
      301 aaagttcatt tggcaaatat ttaatcggaa tcatcaaaaa atatttttca tcaaaatctt
      361 caataaattg taaagatgtt tttttcttcc cttgaaataa gaatttagga ttgtttagaa
      421 cttcaggaag catattcttt gcttttaaga tatcaaattg atctttatgt ttctcgatat
      481 gcttaagtac atcatctgtt gcttgaacta ttttagcact tgatcggctt tgaattattg
      541 gagcaatatt ttctcctatt tctccaatat ttattatctt gcgacctgta tttgataaag
      601 ctggtatgtt cgcaattgtt tgttcgacaa ctgttttttt agtaagtgca cgtatcttac
      661 ccaatccatt aatctgctcc ctttcagatt gccgaatcaa tcccgtttcc ttcacaaact
      721 gccgcatctc tgcttgccat tcgcccattt tcaatcgctc aaaagtgttg tcaagtccag
      781 cagcttcaag cccgccagcc tgtcgcttcc aatatctgat cttgcgctca atccctcgct
      841 gtatctggct cgcgtcatac acactcattt cctgaccctg ataggttacc cgcttacgct
      901 cataagaatc aagctcggaa gaagaataag cattttcgct gattccttca aaaaaaggat
      961 aaaaattgtg cctacagttc caacccatta gtccagggcc agtaccataa ccggtcgtct
     1021 caatgaatgg cttatacttt gggttggttc ctgaccggct ataaacttta ccttgccaac
     1081 tttcatggtt cattggacca acgcctttat tacgagcgcc aatatgagca gagacggcca
     1141 ctaggtcaca gcccatctca tccgcccgcg ctaattgcag gtttccaacc gtctgcccta
     1201 ccccagttag aagcgtgcgc cgcatcgcca catcaagttg atcccggtgc ccgctggcat
     1261 agttgatcgt ggttagtccc tgggaagcca cttgtttgac cgcctgccgc attgcgctgt
     1321 tgtaatcgaa cgccccgctg cttacttcaa gatatactct atctgcaaca gaaataaata
     1381 gctcttgccc agcagaagcg gttgtaagtg taaaattacg cactacccct tgtgtcttgc
     1441 gtaaacccgc ggctaaaact tgcgtcatag caggtgaaag attcaaaggt agaggttgca
     1501 gatctgctgc cttgtaaatt acatcatcga attgaagagt ttttactccg gctttacgaa
     1561 atattctctg caattccaat tcactttttc ccgtcaattg ggaaatacgc tctaatattt
     1621 cctcatagag catcccgctt tcggataatc tttgtaccat ccatgccgct gatgggctgt
     1681 caaagttcat ttttatcaag cggcggacaa tatcaatgat tgcccgctct tcaaattctt
     1741 gataaagatt tatgatgggt tccatcaata cgtcaagctg atcgctcgtt agcaagacgc
     1801 ttttcctccc aatacttttt tttcgtttcg cttaatttgc gtcgcgtttc ctctgtcaag
     1861 cgtttccctt tgtgggcttc cgacatcttt tgtcgcgttt cctggctgtg gttctgtaac
     1921 cccgtcttcc ctttgttcca gggggtttgt cccaatgtgt gtttattgcc aatcaaagag
     1981 gcacttattt ttcgtttgtg ctcattccat cgctttgaaa tattggcact actcccaata
     2041 tatcgatgcc catttaccgt attcacaatc tcatatacgc cacttagtct cttagcggtt
     2101 ttttcgttca tttttcccct atgccccctg aaatagatca gtttcactcg gttgttcatc
     2161 tgtcgcttca gcaacccact ttttcgcctc ttcttctgac agcccgtaat tacgcatgag
     2221 atagcgccat tttgccatac tgcccatgct aaccgtctgt cgatctgtca tcaattgcgc
     2281 atccttatcc acaaccacag aatcatcaaa atcgtaggta acggtgtact tgccttttgg
     2341 tgcgagcgca ttgatcgtcg cccaggtatc catcgcataa atgagatcgt caagggcaat
     2401 cttgatcgcc ttctgcacat cgaccactgt cgagtaactg cgttgcttgc tggttttgat
     2461 ctctgttgcc gtcttgtcca ctatttgcgg atcactcaat gtgccgtaag ccatgccgca
     2521 gttgaactca atgcgttgca agatggtgtt gagtccactc ttgatagata cttcgcgaaa
     2581 ctccggtgac cactcctcat aaaatccagg ttctcctatt tgtgctgact ccatctgatt
     2641 tagcgcgcga tacagccgcc tgtctggtat aattggcttg tcatcagctg tcttgtcaaa
     2701 ggccgacgca tcaacataca gggcgcgttt gccgctctca aattcccaca ttaagtcaga
     2761 ccacaatatg tcagcctgtt tgataaggtc agttgcaccc gcataacacg acacgcctag
     2821 tggagatttt ggatctatat tgttagccag cggatagcgg aaataggcga atagcggctt
     2881 atccaaacca acaatggtcg cttctggctc aagctccttc caatcatcta tcaattccag
     2941 tgacacctga ccacccagta tgtctttagt gctgctcctg tatgcggagt ttctgacaat
     3001 atagccatcg ccggtcatct gatggctctc caggcgcgta taccaataat cgccctgctt
     3061 gcgttggtca ataaatacac aagcggtaat atgtccactg gaatcaaaac tgatcgggaa
     3121 aaattgatca gcatgaatgt agtccacgct gatatttttg ccgtccggat atggtttgaa
     3181 taccagtccg cctttggcgc agccgaactc gatcacctcg cgtatcttac ccatcacatt
     3241 attgaattgt tctgcaagat aatcagcgcg ttttgagcca tcgagcgcaa tagccatttc
     3301 aatggtagcc atgcgcgaaa tttccctggc gatagccgct ggaaggttga gtgatttaac
     3361 ataatcattc agccagttgg atttgttttc gtacattttc gaccacgatt ccagggcttt
     3421 caccattggg tcgctgatgg caatatcttg tccaacagct tgttttatag attgttttcc
     3481 tatcatcttt gtccaccagt ccttaatcca gttgattatt tttgaaaaca tcacaattgc
     3541 tcgctttcgg cactattgac cacggcgacg ccaaattaaa ttgcatgcat accgcactgc
     3601 atcgatggcg tgattattct cgtctgggta atccgatatg taattaccgt cgcgatccgt
     3661 ctcgtgctca taattcaaaa actcttctgc tgaatacggc gcgcgttcgt tgtcgatcac
     3721 aatcgctttc agcgattgca gccatttcat ggagtacctg acgctttccg gacctttttc
     3781 cgcgcctcgg gctgaaactc cgtaatcgcg caaatcagca accgatttcg gctccgcaga
     3841 atcacaaatg agcatatctt gggaagttaa tccatagtcc accagggcac tatatagatc
     3901 ccggttagat gttttccacc tgcgcagctc cccaaaaata tacagcgtca gcctggccgc
     3961 gtcataatgg cagcgcgcat agtgagccgg gtcggggaaa taaccaaaat cctggccatg
     4021 caatacgtgg tcaaactggg taatctcttc gtctgtaatt tttcggattt ccacattatc
     4081 gaagaccaaa cctccgacac cggtcgagac acccatgtac tcatgctcgt aggcgctggg
     4141 gttgatctct ttcaggaact ctgcctcatc gataaacgtt tgcccaagcc attctcgcgg
     4201 tacatctaga taggtcgagt gatgcacgta tcgatcaggc ttgggaatct ccaattcttt
     4261 gagtacccac gaattacgcg acctgggtgg attgaatgat ttgaaaatgt acgccttatc
     4321 accaccacga atggcagatt ggatgatcga acgtaccgcc tcggctccgc ggaattgatc
     4381 cagttcctcg aaccatagaa tattgatata accaaaacgc ggcttgattg acttgatctt
     4441 taatgggtca tcaccaccgc gaaaataaat tttttgccca gtcgggatgt aggttatctc
     4501 gagcgggttg gttgtgcagc gaaatttgtc agccaaaccc agataattaa tcgcccactg
     4561 gagttgcgag aaaacagaat cccgcagagt atcctttact tggcgcgtaa tcagtgcatg
     4621 ccagtccggg ttattaatga tcagctcaat gataacttct gacgtaaagg atgatttcgt
     4681 agacccgcgc ccgcccttga ggacgtattc ggtatgttta tggtcacaga tatcgcgata
     4741 agcgccatag aaacttggag cgattgcatt tgccggtaag tagaacggac cactctctgc
     4801 gtctgactgt tcctgtgctc tatcctcaaa cagtccatat cgcttgccga gcaactccaa
     4861 cgctctcatg cgatcatgca gttcaatttt taatccgttg gctgtcggag taatagatct
     4921 aatcagccgc aacttgccag acttttgtgc tttgccaaaa tcgatatttt ttatacgtcc
     4981 agttccttca tcaatttcta caaaatctcc tatatcggct ctgccaattt ccccgatagc
     5041 ctgaatgacc tcatcggcag acatggcaga ttctgccagg cgaagctgga tttcagctgc
     5101 gatttcaggt ttcttaaggt tttctccccc aattgatcct gcggaacgtt cagagtatcc
     5161 agccagaagt gccgcctgtg ttgcgttcca gcattttaaa tattcatcga tgaaaatccg
     5221 ctgtttgcag cttaaggaca ttacattacc tgttccgcct taagtgcgga ctgaactacg
     5281 ctcgatacat gcttcagcgc atccaattca gctttgatct ctgcatatcg cccgcttatc
     5341 tcgacaaatt cagtctttag ttccaatacc cgaccggcga tcagtaagag caggtcttgt
     5401 ttttga
//
LOCUS       DAOSDS010000132         5382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5426_length_5382_cov_4.057068, whole genome shotgun sequence.
ACCESSION   DAOSDS010000132 DAOSDS010000000
VERSION     DAOSDS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5426_length_5382_cov_4.057068"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /gene="tig"
                     /locus_tag="PLL88_10800"
     CDS             complement(<1..1020)
                     /gene="tig"
                     /locus_tag="PLL88_10800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOJ02095.1"
                     /translation="MEFEKKLLKDHQVQITVNFNQETFEQFKNKAARTISSRSKVPGF
                     RPGKAPLDVVKRIYGEEYLNEEALELLVHEKYPLLLDEAQVKPAAAGKLEKVEKLDPP
                     HLVFIVPLEPVVKLGKYQEIQKKFEPPQIEEKEIDEVIHNLQLNYATAEKVEREAKKG
                     DLVNFKLTALVSNPEKGESDELLKDSPYHMIIGEKQREEFPYEGFDKELVGLKTGDTK
                     ELSHKYSKDSSFEKLQGKEVKLFIETLDVKEMKLPEVTNDFAKSVSGLDTVEMLKTSI
                     KNQLAYTKNADYEEKYYNELIDEIVSRSKISYPPVLLEEETEQLLQNFEHNLSHQNLD
                     LDTFLK"
     gene            complement(1062..1143)
                     /locus_tag="PLL88_10805"
     tRNA            complement(1062..1143)
                     /locus_tag="PLL88_10805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1107..1109),aa:Leu,seq:tag)
     gene            1231..1662
                     /locus_tag="PLL88_10810"
     CDS             1231..1662
                     /locus_tag="PLL88_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02096.1"
                     /translation="MAPVINRNNYANQRDSYLRFISTALLALKNVQNGDDTYKDICVF
                     IYLTLLEIQKSIQKTIQPWEKRGYYVKADQFCAEWQWLEPVLASIGNKIKQQDWKSLA
                     NDLKTVKEICMGISPYQRMQVQDPWRGAWKKWENKSKAAGS"
     gene            complement(1687..3798)
                     /locus_tag="PLL88_10815"
     CDS             complement(1687..3798)
                     /locus_tag="PLL88_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOJ02097.1"
                     /translation="MDIKVMNGLLTPKSVAIIGASSTPGKIGHTVVKNLIESEYEGKI
                     YPVNPNADEILGLKCFHAITEIPADVDAAIITVPAKIVLNSVKECGEKGVKGAIIITS
                     GFSEVGEKAMEEELVKTAHSYGMRILGPNIVGVLSNSDKFNGSFAPFLPLKGSSSLVS
                     QSGALLIGIDAATFMRGVGFDKLISIGNMSDVDFADAIEWLDQDEKTKCISLYIEGFK
                     QGRKVIEAAQKAKKPIIGLKSGVSAHGAAAAASHTGSLAGASKIYDAAFKQAGIIWAD
                     DLNNLFDRTLALSLQPPMKGDNLLVLTNGGGVGVLATDAAEKYGIPLKFAPEDVQAEL
                     KKHMPEFGSAKNPVDLTGMAGTEWYRETVKYSIQHPWVDALVVLYCETAMTNPLEIAQ
                     GIHQALKESGVTDKPVAVSYVGGEKSEEAMRWLVESGIPAYGAPDLAVNAIAALHEFA
                     LNKENNHLEPLETKGFDKNKALAIIQKARQDGRDALTEMEAKQVFEAYQMPVTHSKVA
                     TSEDEAVSLAREIGYPLVMKIVSPQILHKSDAGGVKVNIKDEEGVRNAYNQILKNAKA
                     YKADAEIHGILVQEMAPAGTEVILGSVNDATFGPTVMFGLGGIFVEVLKDVTFRVAPF
                     SKKQALEMMAEIKGAPILRGVRGELPRDVDALADTICKYAHMVLDLADEVSESDANPV
                     LVYEQEKGLKVADARMILKKK"
     gene            3931..5292
                     /locus_tag="PLL88_10820"
     CDS             3931..5292
                     /locus_tag="PLL88_10820"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOJ02098.1"
                     /translation="MNTTLLFESPYFDNHLHANEMVEILQCAFSSVDPCRAVKQAVHF
                     SEAGLQINDTTVLFDARKDKILVWGLGKASQSMARGLKSVLGTKISNGTLITKHANPE
                     LEKFLLPDIVTFEGSHPIPSVKSINAVESSMKRIGKLHNEDLVIALISGGGSALAICP
                     QEGIQLEDYQAMTKALLDCGATIHEINIIRKQIDRIKGGGLAKMLSPARVVSLILSDV
                     IGDPLDMIASGPTVPSKHTKQEARAVIEKYGIKAKVPPAIRSFLEREDRTDTSDLIHG
                     ECEQDIRNFLVGSNVMAANAAKEAALRLGFQTEVVSTNLQGEARTVGSQLGRDLKRKI
                     EEKPGKCCWIYGGETTVTIRGSGKGGRNQELALSAAQELAGVRGGCILSIATDGEDGP
                     TDAAGAVADGDTLRVGASLGLDLQQFLKNNDAYSYFERVGGLIKTGPSGTNVNDLVLL
                     LAF"
BASE COUNT         1396 a         1228 c         1273 g         1485 t
ORIGIN      
        1 tttcaagaag gtgtccagat ccaagttctg gtgagagaga ttgtgttcga aattttgtaa
       61 caactgttcg gtctcttctt ccaaaagaac aggaggatac gagatctttg aacggctgac
      121 gatctcatcg atcaattcgt tataatattt ttcttcgtaa tccgcatttt tcgtataagc
      181 aagctggttt tttatcgacg ttttgagcat ctccacagtg tccaagccgc tgacgctttt
      241 tgcaaagtca ttggtgactt cgggcagttt catctctttc acatccagtg tttcaatgaa
      301 aagcttgacc tctttaccct gcagtttttc aaaggacgaa tctttggaat atttatggga
      361 gagttccttt gtgtcgccgg ttttcaaacc gaccaattct ttgtcaaatc cttcataggg
      421 gaattcttcg cgttgttttt ccccaatgat catatgataa ggagaatcct tcagcagctc
      481 atcggattcg cctttttctg ggttgctgac cagcgcggtc agtttgaaat tgaccagatc
      541 accttttttt gcttcacgtt ctactttttc tgccgtggca tagttgagct gcagattgtg
      601 gatgacctca tcgatttctt tttcttcgat ttgcggtggt tcaaattttt tctgaatttc
      661 ctggtatttg cccaatttaa caaccggttc caatgggacg atgaagacca ggtgcggagg
      721 atctaatttt tcaacctttt ccagtttccc ggcggcagcg ggtttcacct gagcttcatc
      781 aagcaagagc ggatatttct catgaaccag caattccaac gcttcttcgt tgagatattc
      841 ctccccatag attcttttta caacatccag tggggctttg ccggggcgga agccgggcac
      901 ttttgatcga gaagagatcg tccgagcggc tttgtttttg aattgctcga atgtttcttg
      961 attaaaattc acagtgattt ggacctgatg atctttgaga agcttctttt cgaattccaa
     1021 gatatgattt ccttagttaa gttattgata atttttgcct gtggggaagg agggacttga
     1081 accctcacgc cttgcggcac atgatcctaa gtcatgcctg tctgccagtt ccagcacttc
     1141 cccaacagcc aagaaattat atgcgattga tgaaagcgcg tcaatgcaga gtataatctc
     1201 tctttcattt tgttaatatt gaggaaaaag atggcacctg tgatcaaccg taataattat
     1261 gcaaaccagc gcgattcata tctgcgattc atttcaacgg cgcttcttgc attgaaaaat
     1321 gtgcaaaatg gcgatgatac ctacaaagat atctgcgtgt tcatctatct gaccctgctg
     1381 gaaatacaaa aatcgattca aaagaccatc caaccatggg aaaaacgcgg ttactacgtg
     1441 aaggcagacc agttctgtgc ggaatggcaa tggttggaac ctgtgttggc ttcaatcgga
     1501 aacaagatca aacagcagga ttggaaatcg cttgcaaacg atttgaaaac ggtgaaagaa
     1561 atttgtatgg gcatttcccc ctaccagcgg atgcaagtgc aagacccatg gagaggcgcc
     1621 tggaagaaat gggaaaataa aagcaaagcg gctggttcat gatccagccg cttttggttc
     1681 tcaccgttat tttttcttga gaatcatacg agcgtcagcc actttgagcc ctttttcctg
     1741 ttcataaact aacacgggat tggcgtcaga ttcgctgacc tcatccgcaa gatcgagcac
     1801 catgtgggca tacttgcaga tggtatcggc taatgcatct acatcacgcg gcaattcacc
     1861 acgcacgcct cgcaagatgg gggcaccttt gatctctgcc atcatctcaa gggcttgctt
     1921 cttcgagaat ggcgcaacgc ggaaggttac gtcttttaac acctcaacaa aaattccgcc
     1981 caatccgaac atcaccgttg gtccgaaggt ggcatcattg acggaaccca aaatgacctc
     2041 cgttcctgct ggcgccattt cttgaaccag aatgccgtgg atctccgcat cagctttgta
     2101 tgctttcgcg tttttcaaga tctggttgta ggcgttgcgg acgccttctt catcttttat
     2161 gttgacctta acaccaccgg catcggattt gtgaaggatc tgcggcgaaa cgatcttcat
     2221 caccagaggg taaccgatct ctctggcgag gctcacagct tcgtcttctg aagtggcgac
     2281 tttggaatga gttaccggca tttgatacgc ttcgaaaacc tgtttggctt ccatttcggt
     2341 aagcgcatct cgcccatcct ggcgagcttt ttgaatgatt gctaaagctt tatttttatc
     2401 gaatcccttt gtttcaagcg gttcgaggtg attgttttct ttgttgagcg caaattcatg
     2461 caaagcggca attgcattca ccgccaggtc gggggctcca tacgccggga tgccactctc
     2521 aaccagccag cgcatggctt cttccgactt ctcgccaccc acgtacgata ctgcaacagg
     2581 tttatcggta acaccagatt ccttgagagc ctggtgaata ccttgagcaa tctctaaggg
     2641 gttggtcatg gcggtttcac aatacaaaac gaccagggca tctacccaag gatgctgaat
     2701 ggaatatttc acggtctctc tgtaccattc ggtgccagcc atgccggtga ggtcaaccgg
     2761 gttcttggca gaaccgaact cgggcatgtg tttctttaat tctgcctgta cgtcttcagg
     2821 agcaaatttc agagggatgc catacttttc agcagcatct gtggctaaaa cccccacacc
     2881 acctccgttt gtaaggacaa gcagattgtc ccctttcatc gggggctgca aggatagggc
     2941 gagtgtgcga tcgaacaagt tattgagatc atctgcccaa atgatgcccg cctgtttgaa
     3001 tgccgcatca taaatttttg acgcaccagc caaagagccg gtatgcgaag cggcagcggc
     3061 agcgccatga gccgaaacgc cggatttcag accgatgatc ggtttttttg ctttttgagc
     3121 cgcttcgatc accttgcgac cttgtttaaa cccttcaatg tagagggaaa tacatttggt
     3181 cttttcatcc tgatcgagcc attcgatcgc atccgcaaaa tcgacatcgg acatattgcc
     3241 aatggagatc aatttatcga aacccacacc gcgcataaac gtggcggcgt caatgccgat
     3301 caacaatgcc ccactttggg agaccaggga actgcttccc tttaagggca ggaagggtgc
     3361 gaaggatccg ttaaacttat ctgaattgga aagcacaccc acaatgttgg gacctaaaat
     3421 ccgcatccca taagaatgag ctgttttaac caattcctct tccattgctt tttcaccaac
     3481 ttcgctaaat cccgacgtga tgatgatggc gcctttcacc cctttttcac cacattcttt
     3541 aacggaattt aaaacgatct tggctgggac ggtgatgatt gctgcatcaa catcagcggg
     3601 gatctccgtg atggcatgga aacatttcaa tcctaaaatt tcatcagcgt tagggtttac
     3661 cgggtagatc tttccctcat attcactttc gatgagattt tttaccacgg tatgcccgat
     3721 ctttcctggg gttgaggatg cgccaatgat tgcaacggac ttgggtgtca gcaagccatt
     3781 catcactttg atatccacag taaaacctcc tgcacaactt gatagttttt gatagttttc
     3841 agaaaaaatc cgaacacaag tcataatttt acactcttta taaaaaaact gcagcgaaga
     3901 taatggaaca tttgtgatta gaatattgtt atgaatacaa cgcttttgtt tgaatcacca
     3961 tattttgaca accatctcca tgcaaatgag atggttgaaa tactccagtg cgctttttcc
     4021 agcgtagatc catgcagggc ggtgaagcag gctgtgcatt tcagtgaagc gggacttcaa
     4081 ataaacgata caaccgtcct gtttgatgca agaaaagaca aaattctggt ttgggggttg
     4141 gggaaggcgt cacaaagcat ggcacggggt ttaaagagcg ttcttgggac gaagatcagc
     4201 aacggaacat tgatcaccaa gcatgccaat cctgaacttg aaaaattctt actgccagat
     4261 attgttacat ttgaaggatc gcacccaatt ccctcggtaa aaagcataaa tgccgtagaa
     4321 tcgtccatga agcggatcgg aaaattgcat aatgaagacc tagtcatcgc actcatttca
     4381 gggggtgggt cagcgttagc gatctgccca caagagggca ttcaacttga agactatcag
     4441 gcgatgacga aagcattgct tgattgtggg gcgacgatcc acgaaatcaa tatcattcgc
     4501 aaacaaatcg atcggatcaa gggaggggga ctggcaaaga tgctctcgcc tgcacgtgtt
     4561 gtctcactga tcctttctga cgtgatcgga gatccattag atatgattgc gtcaggacca
     4621 accgttcctt ccaagcatac caaacaggaa gcgcgtgcgg tgatcgagaa atatggaatt
     4681 aaagcaaagg tgccaccagc cattcgttcc tttcttgaga gagaagatag aacggatacc
     4741 tcagacttga ttcacggtga atgtgagcag gatatacgaa atttccttgt tggcagcaat
     4801 gtgatggctg ccaatgcagc caaggaagct gctctgcgat tgggttttca aactgaagtg
     4861 gtatccacca atctgcaggg agaagctcgc acagttggtt cgcaattggg cagagatctc
     4921 aaaaggaaaa tagaagaaaa gccgggaaag tgttgctgga tttatggtgg agaaacgacc
     4981 gtgacgattc gggggagtgg gaagggaggg agaaatcagg agttagctct ttcggcagcg
     5041 caagaactcg ccggggtgcg agggggatgt atcctatcca ttgccacgga tggcgaggat
     5101 ggaccaaccg acgcggctgg tgcggttgct gatggagata ctctacgcgt aggagccagc
     5161 ctgggtttgg acctgcaaca gttcttgaaa aataatgatg cttattccta ttttgaaaga
     5221 gtgggtggat tgatcaaaac aggtcccagt ggtacgaatg ttaatgatct tgtcctgtta
     5281 ttggcttttt gattgataca gtgccttgac ggatggaagc aaatagtcat gaaaagaccg
     5341 ctgcatatca taatcgggtt tccaacccca atcattgtgg gc
//
LOCUS       DAOSDS010000133         5363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5449_length_5363_cov_4.329879, whole genome shotgun sequence.
ACCESSION   DAOSDS010000133 DAOSDS010000000
VERSION     DAOSDS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5449_length_5363_cov_4.329879"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PLL88_10825"
     CDS             complement(<1..402)
                     /locus_tag="PLL88_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02099.1"
                     /translation="MVAAYPILEFDPDPQAILSPQVEALKGKLPRKAVMCFFQDVLRE
                     KFSEGKITKVGQLRSEIGENPVYTMPVGGQSLTVLHAGDGASLAASFLDDLIAAGVQQ
                     VIACGGCGALVENIPAGHVLIPTSAVRDEGTS"
     gene            complement(423..1259)
                     /locus_tag="PLL88_10830"
     CDS             complement(423..1259)
                     /locus_tag="PLL88_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOJ02100.1"
                     /translation="MENRVYFGDNLPILGSLPDASVDLIYIDPPFNTGHTQTRTRIRT
                     VSSEQGDRTGFKGQRYETIQLGKNGYADTFDDYLAFLEPRLREAYRILKAHGSLYFHI
                     DYREVHYCKVLLDRIFGRDSFINEVIWAYDYGARTQKRWPPKHDNILWYAKNPAHYTF
                     NYQAIDRIPYMAPGLVGKEKAARGKLPTDTWWHTIVPTNSHEKTGYPTQKPVGVIRRI
                     VTASSNAGDLVLDFFAGSGTVGEVCLKLKRNFILIDNNPQAFQVMKKRFQDDPSIEWL
                     EK"
     gene            complement(1263..1940)
                     /locus_tag="PLL88_10835"
     CDS             complement(1263..1940)
                     /locus_tag="PLL88_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOJ02101.1"
                     /translation="MYGIMLPTDSLPAIFFFAFSMAIGAVLSPGPVTMALISQSPRLG
                     WITGPLVSTGHALTELLMAGMITFGLATFLSQPTLQTAIALLGGILLLWMGAGFVLSG
                     MKGKYHLPTQEEKETTQPMSRGQALTMGVLTTITNPFWYAWWITVASVYLLEAKAMGM
                     APVVAFYLGHISVDYLWNTLLSTVVGSGGKLFSDRVYNILMAICGSYLLYLGVQFLTV
                     GIKGLPD"
     gene            2031..2720
                     /locus_tag="PLL88_10840"
     CDS             2031..2720
                     /locus_tag="PLL88_10840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOJ02102.1"
                     /translation="MKEKDFATYLQEWVSALPSVDGGALFQHSENTAILCVDVTNGFC
                     KSGALASPRVAGIIPPIVRLFKQAWQSGCRDFLLLQDTHEPDAVEFGAFPPHCVRGSQ
                     EAQTVSEISDLPFSAQFNTMEKNSINSALNTSLDAWITAHPQITTYIVVGDCTDLCTY
                     QLAMHLRISANAFQKQRRVVVPANCTDTYDTPVTLTEQIGAFAHPADYFHNTFLYHMA
                     LNGVEVAREIQ"
     gene            complement(2769..3719)
                     /locus_tag="PLL88_10845"
     CDS             complement(2769..3719)
                     /locus_tag="PLL88_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02103.1"
                     /translation="MKKTVIIILLIALFAFSGCTLLDKFEEVKQRYLTNRVEQLLTEI
                     PSEEVVEEANKESGSESAQVTATSAVKDAIATAAAQATAQAEEVALAAAVTAEPTAEP
                     TVAPTNAPTPTPTIASTDPGVYLGIADWTDDMDNIKNWPVDTNDFSSAAHQNGYYRLT
                     SLAEADGWRLANTEGVTNGYVELVFTTETCSADDHYGIIFRVPVLREANRGYLFGVTC
                     DGRYNLRKWDGTTGEQGTMTTLVRYTYSGMINAGSNKQNVLGVMTKGNLISLYINGEL
                     INQVTDDTYTSGYFGAFVGWEKTANFTVHVDKASYWVEAE"
     gene            complement(3743..4228)
                     /locus_tag="PLL88_10850"
     CDS             complement(3743..4228)
                     /locus_tag="PLL88_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOJ02104.1"
                     /translation="MEIKSIAIEKPEEINFILGQSHFIKTVEDIHEVLVSTVPGIQFG
                     LAFCEASGKCLVRWSGTNEAMIALAKKNATAIAVGHSFILFLAEGFFPINVLNAIKAV
                     PEVCHVFCATANLAHVIIAEEAGGRAILGVVDGESPKGIEGPNDIAWRKEFLRKIGYK
                     A"
     gene            4396..5310
                     /locus_tag="PLL88_10855"
     CDS             4396..5310
                     /locus_tag="PLL88_10855"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOJ02105.1"
                     /translation="MFELIFLGTSASAPSIQRGLPAQVVKHNEHRFLVDCGEGTQRQI
                     LRSGLGFKKLNRLLITHGHLDHILGIGGLVSTFLRWETIENLEIYGGDHTLERIEDLL
                     FRVVIRGVTPPFPIELKTIKKGPIIAEKDFTISAFPVKHRSAGCFGFTFEEESKRPFL
                     AEKADALGIPSGPMRRDLVQGKAVVLENGTRVVPDDILGKPAPGTKLVIVGDTGETKS
                     LEKVCQNADALVIEGTYLYEEVELAREYAHLTVREAAELAAKVGVKQLFITHVSRRYR
                     NKDVEKEAQTIFPDSVVVKDFDQFQIKN"
BASE COUNT         1316 a         1441 c         1358 g         1248 t
ORIGIN      
        1 agaggtgcct tcatcgcgca ctgcactggt gggaatgagc acatgccccg ccgggatatt
       61 ttccaccagc gccccgcacc cgccacatgc gatcacctgc tgcacaccgg ctgcaatcag
      121 gtcgtccaga aagctggctg ccagcgaggc gccgtctccc gcatgcagga cggtgaggga
      181 ctgtcctccg accggcattg tgtaaaccgg attctctccg atctccgagc gcaattgacc
      241 aaccttcgtt atctttcctt cagagaactt ctcgcgcagc acatcctgaa agaaacacat
      301 cactgccttg cggggcagtt ttcccttcag cgcttcgact tgcggagaaa ggatagcttg
      361 tggatcgggg tcaaattcaa gaatgggata tgcagcgacc atgcaagaat gctttcttct
      421 ctttattttt ccaaccattc aatggacgga tcgtcttgga agcgtttttt catcacctga
      481 aatgcctgag ggttgttatc gatcaggatg aaatttcgtt tcagtttcag acaaacctcc
      541 cccaccgtgc cgctgccggc aaagaaatcc agcaccaggt caccggcatt ggaactggca
      601 gtgacgatgc gtcggatgac gcccactggt ttttgcgtcg gataaccggt tttctcatga
      661 ctgttggtgg gcacgatggt gtgccaccag gtatcggtag gcaatttgcc gcgcgcagcc
      721 ttttctttcc ctaccagccc gggcgccatg tacggaatgc gatcaatggc ctggtaatta
      781 aaggtgtagt gagccgggtt tttggcgtac cacaggatat tgtcatgctt gggcggccag
      841 cgtttttggg tacgcgcgcc ataatcataa gcccagatga cctcgttgat aaaagaatcc
      901 cgcccgaaga tccggtctaa caggactttg caataatgca cctcgcggta atcaatatgg
      961 aaatacagcg agccatgcgc tttcagaatg cggtaggctt ctcgcaggcg cggttccagg
     1021 aatgccagat aatcatcgaa ggtatccgca taaccattct tgccaagttg gatggtctca
     1081 tagcgctgac ctttaaaacc ggtacggtca ccctgctcgg aagaaacggt gcggatgcgc
     1141 gtgcgggttt gggtatgccc ggtgttgaaa ggcgggtcga tatagatcag atccaccgaa
     1201 gcgtccggta atgagcctag aatgggcaag ttgtccccga aatagaccct gttttccatt
     1261 tgttaatcgg gcaacccttt gataccgacc gttaagaatt gaactcccaa atagagcaga
     1321 tatgatccgc aaatcgccat aaggatattg tacacgcgat cgctgaacag ttttcccccg
     1381 ctacccacca cagtagaaag caaagtattc cataaataat ctacgctgat atgccccagg
     1441 tagaacgcga ccaccggcgc catacccatt gccttggctt ccagcaaata taccgatgcc
     1501 accgtgatcc accaggcata ccaaaaggga ttggtgatgg tggtcagaac acccatggtc
     1561 aaggcttgcc cgcggctcat cggttgggta gtctcttttt cctcttgagt gggcagatgg
     1621 tatttccctt tcataccact caacacgaac cccgccccca tccacagcag caagatgccg
     1681 cccagcagcg caatggctgt ttgcagggtt ggctgtgaaa ggaaggtagc caagccgaaa
     1741 gtgatcatgc ctgccatgag cagctcagtg agcgcgtgcc ctgtcgaaac cagcggtccg
     1801 gtgatccaac ccaggcgagg ggattgggag atgagcgcca tggtaaccgg tccgggggaa
     1861 agtacagccc caatagccat gctgaaagca aaaaagaaaa tggcgggaag ggaatcggtt
     1921 ggcaacataa tgccgtacat tgtagcataa cccttcaaaa agtgagtgag ctggtttatg
     1981 attgcagtga tcccgaaagg atacgagttc gaaagcaggt aaaaagaaca atgaaagaga
     2041 aagattttgc aacgtattta caagaatggg tatcagcgct gccatccgtt gatggaggag
     2101 cactttttca gcactctgaa aacaccgcca tcttgtgtgt ggatgtgacc aacggctttt
     2161 gtaagtctgg agcgctcgcc agtcctcggg tagccggcat cattccacct atcgtacgct
     2221 tgttcaaaca agcctggcaa tctggctgca gagattttct actcctccag gacacccacg
     2281 aaccagatgc ggtggaattc ggggcatttc caccccattg cgtgcgcggc agccaggaag
     2341 cgcaaaccgt ctcggagatc agcgacttgc ccttctccgc tcaattcaac accatggaaa
     2401 agaactccat caattctgcg ctgaacacca gtttggatgc ctggatcacg gcacacccac
     2461 agatcaccac gtatattgtg gtgggggatt gcaccgacct atgcacttac cagctcgcca
     2521 tgcacctgcg catcagcgca aacgctttcc aaaaacagcg ccgggtggta gtgcctgcca
     2581 actgcaccga tacctacgat acacctgtaa cactgacaga acaaatcggc gctttcgccc
     2641 atccggctga ttatttccac aacactttcc tgtaccatat ggctttgaac ggcgttgaag
     2701 ttgccagaga aatacagtaa aaaaagtgcc tgagcgacgc tcaggcactt tttattagac
     2761 tgttcttttt attcagcttc cacccagtag ctggctttat cgacatgcac ggtgaagttc
     2821 gcagtctttt cccaacccac gaatgcgcca aaataaccgc tggtgtaggt gtcatccgtg
     2881 acctgattga tcagttcgcc gttgatataa agactgatga ggttgccttt ggtcatcacg
     2941 cccagcacat tttgcttgtt ggagcctgca ttgatcatcc cactgtaggt gtagcggacc
     3001 aacgtggtca tcgtaccctg ctctccggtg gtaccatccc atttacgcag gttgtagcgc
     3061 ccatcgcaag tgaccccaaa gagataaccg cggttagctt cccgcaacac gggaacgcga
     3121 aagatgatgc catagtgatc atccgcgcta caggtttcag tggtaaacac caattcaaca
     3181 tacccgttgg tcacgccctc cgtgttcgcc aggcgccagc catcagcctc agccagcgag
     3241 gtgaggcggt aatagccatt ctgatgggca gccgagctga aatcattggt atcaaccggc
     3301 cagttcttga tgttgtccat gtcatccgtc caatcggcaa tccccaagta cactcccgga
     3361 tctgtggaag cgatggttgg cgtgggggtt ggcgcgttgg taggtgcgac agtgggttct
     3421 gcggttggct ctgcggtcac ggctgcggcg agagcaactt cttcggcttg agcagtcgcc
     3481 tgggcggctg cggtggcgat ggcgtcctta accgctgagg ttgcggtgac ctgtgcgctt
     3541 tcgctcccac tttctttgtt cgcctcttcc accacttctt cagagggtat ttcggtaagc
     3601 agttgttcaa ccctgttggt taaatagcgc tgcttgacct cttcgaattt atccaacagc
     3661 gtgcagccgc tgaatgcgaa caaagcgatc aataagatga tgataactgt cttcttcatt
     3721 tttctctcct ctttccaatg ggtcatgcct tatacccgat tttgcgtaaa aattccttcc
     3781 gccacgcgat atcgtttgga ccttcgatgc ctttaggaga ttctccatcc accaccccca
     3841 gaatagcccg cccacccgct tcttccgcta tgatcacatg ggcgagattg gcggtggcac
     3901 aaaagacatg gcagacctct ggaacagctt tgatagcatt gagcacattg atagggaaaa
     3961 agccttcagc caggaaaagg ataaaactat gcccgactgc aatggcggtt gcgttctttt
     4021 ttgccagcgc tatcatggct tcgttcgtcc ccgaccagcg caccagacat ttgcccgaag
     4081 cctcgcagaa agccagaccg aattgaatgc ccggcaccgt gctcaccaga acttcatgga
     4141 tatcctccac ggtcttgata aaatgcgatt gccccaggat aaaattgatc tcctccggtt
     4201 tttcgatcgc gatgcttttt atctccatgc catgctcctt taaaaggtat agaagtcaac
     4261 aaatatagtg caagattgat accaaccaca atgcaatgct gatatcttaa tcatacactc
     4321 tgttatattg atatagaaaa tcgaggatga gatagagtac aataataacc tcaaaaacca
     4381 acaggaaagc acactttgtt tgaactgatc tttctaggaa cctccgcctc ggctccctcc
     4441 attcaacgag gtctgcctgc acaggtggtg aagcacaatg aacaccgttt tttggtggat
     4501 tgcggagaag gcacccagcg tcagatcctg cgatcggggc tgggattcaa aaagctgaac
     4561 cggctgctca tcacgcacgg gcatttggac catattctag gcatcggtgg gctggtttca
     4621 acctttttac gctgggagac cattgaaaac ctggagatct atggcgggga tcacaccctg
     4681 gaacgcatcg aagacctgct ctttcgtgtg gtcatccgcg gcgtgacccc acccttcccg
     4741 attgaattga aaacgatcaa gaaagggccg atcatcgcag aaaaagactt taccatttcc
     4801 gcttttccgg tcaaacatcg cagcgcaggc tgctttggtt tcaccttcga agaggaaagc
     4861 aaacgacctt tcctagctga aaaagcagat gcactgggta ttccatccgg acccatgcgc
     4921 cgcgacctgg tgcagggcaa agcggtggtg ctggaaaatg gtacgcgcgt ggtgcccgat
     4981 gacattctgg gcaaacccgc ccccggcacc aaattggtga tcgtgggtga taccggcgag
     5041 accaagtcat tggaaaaagt atgccagaac gccgatgcgc tggtcatcga aggcacctat
     5101 ctgtatgaag aggtcgaact ggcgcgtgaa tacgcgcacc tcaccgtgcg cgaagccgcc
     5161 gaactggcag ccaaggttgg cgtcaaacag ctgttcatca cccatgtttc gcgacgctac
     5221 cgcaacaagg atgtggagaa ggaagcacaa actatctttc ccgatagcgt ggtggtcaaa
     5281 gatttcgacc agttccagat caagaactaa tttcgaaaca aagaagcagg aattgcttgc
     5341 ggtaaaagga acggctgggg tat
//
LOCUS       DAOSDS010000134         5310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5518_length_5310_cov_3.985538, whole genome shotgun sequence.
ACCESSION   DAOSDS010000134 DAOSDS010000000
VERSION     DAOSDS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5518_length_5310_cov_3.985538"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="PLL88_10860"
     CDS             <1..430
                     /locus_tag="PLL88_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HOJ02106.1"
                     /translation="KKNILQFVNDLITIFQIKTPSTDTPVRLLSGGNQQRLILAREIT
                     ASQGILIAVYPSRGLDVGATESVRSTLLAQREKGATILLISEDLEEIKQLSDRVIVLY
                     EGKIMGTVDPQSTPLETIGLMMAGQTLTNIEKGEEEKQSA"
     gene            427..1518
                     /locus_tag="PLL88_10865"
     CDS             427..1518
                     /locus_tag="PLL88_10865"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ02107.1"
                     /translation="MRIQFEKRTDQNKISAFLIPLASFLLALLFCATILIFFGIDPIQ
                     AYSVMVKGSLGSSYALSETLVKAIPLMLTGLGVSFAFHMHFWNIGAEGQLAMGGIAAA
                     YVALFLKDVIPPSLLLPAMFLAGGLAGAIWGLVPAGLKATIGVNEILTTLMMNYIATL
                     LVEYLYLGPWRDPKGFGFPGTAQFVTEAWLPRLTGRVHYGVFFAILGAILIWFILKYT
                     KRGYEIRLIGENPKAARYAGINIPKNIILVMLISGGLAGIAGMAEVSGISHRLYTGLT
                     VGYGYTAIIIAWLAALNPWAILLVAFFMGALIVGGDQLQIAMQTPAAVADILQGAILF
                     FMLGGSIFNQYKIIITRRRSRKHGEKVHE"
     gene            1511..2467
                     /locus_tag="PLL88_10870"
     CDS             1511..2467
                     /locus_tag="PLL88_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ02108.1"
                     /translation="MNEAFVISILVITIRAGTSLLYATFGAILTERSGIQNLGVEGIM
                     MVGAAASFAVAYHSNSVWLGLAGAVLVGVILGCIHAFLTITLRAEQVVSGLAMTIFGT
                     GLASFMGQRLGPGGTTMVGLQGAAFKRLPVPILREIPYIGEAFFNQDIMVYFLYLLIP
                     LIWLYLYKTRQGLELRAVGENPQAIDALGMDVTKMRFIYTVVGAIFMAVAGAHLSLGY
                     LQGWADNITGGRGWIAIALVIFATWNPLRAVLGALLFGGINAIQFRMQAAGTTLPSSF
                     LNMLPYIVTIVVLVIITWAERRSKKLGTPSALGIPFVREEKK"
     gene            complement(2520..2939)
                     /locus_tag="PLL88_10875"
     CDS             complement(2520..2939)
                     /locus_tag="PLL88_10875"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOJ02109.1"
                     /translation="MLTQEKKFELLKLAVKGSENAHCPYSHFRIGAAVLGKSGKVYLG
                     GNIENVSYGATVCAERVAIWKAVSEGEKEFEAIAVVGPGSEEAYPCAQCRQVMVEFGL
                     DWLVISGDAQGNYLGERTVREIVPFPFVPSALLGEEK"
     gene            complement(2975..3487)
                     /locus_tag="PLL88_10880"
     CDS             complement(2975..3487)
                     /locus_tag="PLL88_10880"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ02110.1"
                     /translation="MIKLEEMSPGDFDNYLLCSIPHYVQELEATGEFTAEQAEEYAQR
                     QFQELLPDGLASPGQYFFNILDDERGCKVGIVWVNVKTENDKTSAFIYDLEVKDGERG
                     KGFAAHTMLQVDEFSKEKGATEICLHVFANNVIAFHLYEKLGYRIRKTFYKKDGSTVM
                     SFRMAKSFLE"
     gene            3670..4791
                     /locus_tag="PLL88_10885"
     CDS             3670..4791
                     /locus_tag="PLL88_10885"
                     /inference="COORDINATES: protein motif:HMM:NF021235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOJ02111.1"
                     /translation="MKKYGFLVFLILVAALLTGWIYSINARKSQSEPVGQISAKWWSS
                     AHADISAEAFTHWNEDEPAVVPANCAKCHSGQGFIDYLGQDGSTAFNVDEPAAIESVI
                     SCEVCHSEKAAQLDTVPYPSGAEVSFNINDALCATCHSGTDAGEAVLEATTGFGDDEL
                     VPDGSFITPHYAFASATWLGSEGKGGYEYVGKEYVGRFEHASGVQTCTQCHDAHSLRM
                     NTVQDGTDLCSTCHSNVTDYSDYRDITFWGTDYDNDGTAEGMYHEIEGVREILYRAMQ
                     QYGTNVLGEGIGWLDRYPYLFKDTNGDGVISDDEAAFSNAYQTFSPRLMRSAFNLQFS
                     LKDPAGYVHNGKYVLQLLFDSIEDLAAVVTVEYNPSLRP"
     gene            4834..>5310
                     /locus_tag="PLL88_10890"
     CDS             4834..>5310
                     /locus_tag="PLL88_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HOJ02112.1"
                     /translation="MTKKTKGFLYTLIYLLLIFLPLLVFLAFSRSLVRSFWRELSVML
                     GFVGLCMAGLQLVPTTRLPFLADVFDLGKVYKVHHLLSIFSVILVLLHPVILLINNPN
                     IIILFNAFTAPWSIQAGWIGVASLILIALTSIYRKQLRIGYNAWHIIHVLFTFAIVV"
BASE COUNT         1180 a         1430 c         1279 g         1421 t
ORIGIN      
        1 aaagaaaaat atcctgcagt tcgtaaacga tttgattacc atcttccaga tcaaaacccc
       61 atcgaccgat acacccgttc gcttgctttc cggaggaaat cagcagcgct tgatcctggc
      121 acgcgagatc accgctagcc agggcatact catcgcggtc tatccaagcc gcggcttgga
      181 tgttggcgca actgaatccg tgcgctctac tttgttggca cagcgtgaaa aaggtgccac
      241 aatccttttg atctcggaag acctcgagga gattaaacag ctttcagacc gcgtcattgt
      301 tctctatgag gggaaaatca tgggcactgt tgaccctcaa tccacccctc tcgagacaat
      361 cgggctgatg atggctgggc aaacgctcac aaatattgaa aaaggtgaag aggagaaaca
      421 aagcgcgtga gaattcaatt cgaaaaaaga accgatcaga acaaaatatc cgctttcttg
      481 atcccgcttg cctcgttcct tctcgccctc ctcttttgtg cgaccatcct cattttcttc
      541 ggaattgacc ccatccaggc atattcggtc atggtgaaag gttctctcgg cagctcgtat
      601 gccctatcgg aaacccttgt aaaagccatc cccctgatgt taaccgggct gggtgtttcg
      661 ttcgctttcc acatgcattt ttggaatatt ggcgctgaag gtcagcttgc catgggtggg
      721 atcgcggctg cttacgttgc actgttcctc aaagatgtca tcccgccctc gcttcttctt
      781 ccagccatgt tcctggctgg agggttggcg ggtgcaatct ggggattggt tcctgccggt
      841 cttaaagcaa ccatcggagt taacgagatc ctgaccacgt taatgatgaa ctatattgcc
      901 acactgctgg tcgaatatct ctacctgggt ccatggcgcg atcccaaagg ttttgggttc
      961 ccgggcacag cccaattcgt gactgaagcc tggttgccgc gcctcaccgg acgcgtgcac
     1021 tacggcgtct tctttgccat cctgggcgcc attctcatct ggttcatcct taaatacacc
     1081 aaacgcggat acgaaattcg cctgatcggc gagaatccca aagctgcccg ctatgcaggg
     1141 atcaacattc ccaaaaatat catccttgtc atgcttatct ccggggggct tgccgggatt
     1201 gctggcatgg cagaggtttc gggtatctcg caccggcttt acaccggctt gacggttgga
     1261 tacgggtaca ccgccatcat catcgcctgg ttggctgcct tgaatccctg ggcgatcctt
     1321 ttggtggcgt tcttcatggg cgcccttatt gttggtggcg atcaactgca aatcgccatg
     1381 caaaccccgg ctgcggtggc agatatcctg cagggtgcca ttctgttctt tatgcttggt
     1441 ggttccattt tcaatcaata caagatcatt atcacccgac ggcggtcgag aaaacacgga
     1501 gagaaagtgc atgaatgagg cttttgtcat ttccatcctt gtgattacca tacgcgcggg
     1561 cacctccttg ctgtatgcca cctttggcgc catattgaca gagcgttctg gcatccagaa
     1621 tctgggcgtg gaaggcatta tgatggtggg tgccgcagct tctttcgcgg ttgcctacca
     1681 ttccaacagc gtttggctcg gtttggcagg cgctgtgctg gtgggggtca ttctgggctg
     1741 cattcacgcc tttctcacca tcaccttgcg tgccgaacag gtggtcagtg ggctggcaat
     1801 gaccatcttt ggcaccggct tggcaagctt catgggtcaa cggttggggc ccggtggcac
     1861 caccatggtc ggtttgcaag gggctgcttt caaacgtctt cctgtcccga tcttaagaga
     1921 gatcccatat atcggagaag ccttctttaa tcaagacatc atggtgtatt tcttgtatct
     1981 tctcatccct ttgatctggc tctacctata caaaacccgc caggggttgg aactaagggc
     2041 agttggtgaa aatccacagg caattgatgc attgggtatg gatgtgacaa aaatgcgttt
     2101 catctataca gtggttggag ctatctttat ggcagtggct ggcgctcacc tttcgcttgg
     2161 atacttgcag ggttgggcag ataacatcac tggcgggcgc ggttggatcg ccatcgctct
     2221 ggtgatcttt gccacctgga accccttgcg cgctgtgctt ggcgcgctgc tgtttggagg
     2281 tatcaatgcc atccaattcc gcatgcaggc tgctgggacg accctgccgt catctttcct
     2341 gaatatgctc ccctatatcg tcaccatcgt agtactggtg atcatcacct gggcagaacg
     2401 ccgttcaaag aagttgggca cacccagcgc attgggcatt cccttcgtgc gcgaggagaa
     2461 aaaatagaag aggaactccc tggagaagtt cctcttcact tcctatgctt ttcaagcagc
     2521 tatttctctt cgcccaacaa agcggatggg acgaagggaa aaggcacaat ttcacgcacg
     2581 gttctttcac ccaaataatt tccttgtgcg tcaccactga ttaccagcca atccagccca
     2641 aattccacca tcacctgccg gcactgggcg caggggtatg cttcctcgct tcccggacca
     2701 accacagcga tcgcttcaaa ttctttctca ccttccgaaa ctgccttcca gatggctacc
     2761 cgttcggcac aaacggtggc gccataagag acattctcga tatttccgcc caggtacact
     2821 ttccctgatt ttcccaacac ggctgcacct atcctgaaat gagaataagg acagtgtgcg
     2881 ttttcagatc cctttaccgc tagtttcaat aattcgaatt tcttttcttg cgttaacatg
     2941 cttggtcctt tcgttcgttg atatttttaa tcgatcactc aagaaaactc ttcgccatgc
     3001 gaaaactcat aactgtgctt ccgtcctttt tatagaacgt ctttcggatg cgatacccca
     3061 gtttttcgta aagatgaaag gcaatgacat tgttggcaaa cacatgcaga cagatctctg
     3121 tcgccccttt ttcttttgaa aattcatcta cctgcagcat ggtatgcgcg gcgaaccctt
     3181 ttccccgctc gccgtccttg acctccagat catagataaa ggcagaggtt ttatcgtttt
     3241 ccgttttgac gttgacccac acgatcccga ccttgcatcc ccgttcgtca tccaaaatgt
     3301 tgaaaaaata ttgccctggt gaagccagtc cgtctggtag caattcctgg aactgccgtt
     3361 gtgcatactc ttcagcttgt tccgccgtga attcgccggt ggcttccagc tcctgcacat
     3421 agtggggaat ggaacacaga agataattgt cgaaatcgcc gggactcatt tcttccagtt
     3481 tgatcacgga ttcgttttcc tgtctcacaa ttctgtatta agtttactcg ctttctcgcc
     3541 tggcgcgtca taaagttgac aaatttcagg ggaattagat tttttttaga aaaatttgct
     3601 acaataggat cattggtatg agagcttcct tctcttggct gatggaaaca accaaccatg
     3661 gaggtcccta tgaaaaagta cggttttctt gttttcctca tcttggtagc tgcacttctg
     3721 accggctgga tctattccat caacgcccgc aagtctcaga gcgagccagt gggccagatc
     3781 agcgcgaaat ggtggagctc agcacatgct gatatttcgg cagaagcttt cactcactgg
     3841 aatgaggatg agccagccgt tgttcccgca aattgtgcca aatgccacag cgggcagggt
     3901 ttcattgatt accttggaca ggatggcagc actgctttca acgtagacga accggcggca
     3961 atcgaatcgg tcatttcttg cgaggtttgc cacagtgaaa aagccgccca gttggatact
     4021 gttccctacc cctccggtgc ggaggtctca tttaatatca acgatgccct atgcgccacc
     4081 tgccacagcg gcacggatgc cggcgaggca gtgctagaag ccaccaccgg ctttggggat
     4141 gatgaactcg tgcccgacgg cagcttcatc acaccccatt atgcttttgc gtctgccacc
     4201 tggctgggca gtgaaggaaa aggcgggtat gaatacgttg gcaaagaata tgttggacgt
     4261 tttgaacatg ccagcggagt gcaaacctgc acgcaatgcc atgacgccca cagcctgcgc
     4321 atgaatacag tccaagatgg caccgacctg tgctccactt gccattccaa tgtgacggat
     4381 tacagcgatt accgcgacat taccttttgg gggacagact atgacaacga tggaacagcg
     4441 gaaggcatgt accacgaaat tgaaggtgtg cgcgaaatcc tgtacagagc aatgcagcag
     4501 tacggcacca atgtgctggg cgaaggcatc ggctggctag accgctaccc ctacctgttc
     4561 aaagatacca acggtgatgg agtgatctca gacgatgaag cagctttctc caatgcgtat
     4621 caaacctttt cgccacgcct gatgcgctct gctttcaatc tgcaattcag cctcaaagac
     4681 ccggctggct acgtgcataa tgggaaatat gtcctgcaac tgctctttga ctcgatcgaa
     4741 gatctggctg cggtggttac ggttgagtac aacccctcgc tccgtccatg atcgagcgtc
     4801 tttttccttg aactgaaata aagaaggtaa accatgacaa agaaaacaaa aggctttctc
     4861 tataccctga tctacctgct ccttatcttc ctgccgcttc tggtttttct ggcattctca
     4921 cgttctctgg tgcgctcctt ctggcgcgaa ctttcagtga tgttgggttt tgtggggctt
     4981 tgtatggctg gtttgcaact cgtccccacc acgcgtcttc cctttctggc agatgtattt
     5041 gatctgggca aagtatacaa ggtgcatcac ctgctttcga tcttttccgt catactggtg
     5101 ttgctgcacc ctgtgatctt gctgatcaac aatccgaaca tcatcatcct gttcaacgca
     5161 ttcaccgctc cctggagcat ccaggctggc tggatcggag tggcaagcct gattctgatc
     5221 gcccttacct ctatctatcg caagcaattg cgcattggct ataacgcctg gcacattatt
     5281 cacgtcttgt tcacctttgc catcgttgtg
//
LOCUS       DAOSDS010000135         5198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5668_length_5198_cov_5.392767, whole genome shotgun sequence.
ACCESSION   DAOSDS010000135 DAOSDS010000000
VERSION     DAOSDS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5668_length_5198_cov_5.392767"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="PLL88_10895"
     CDS             <1..546
                     /locus_tag="PLL88_10895"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HOJ02113.1"
                     /translation="STAIVQLAKKYGICSDVYGLGTSAFAFDPQNGFEKSLNALLPVL
                     AGANLIAAAGLMEDALTSSYEQLVIDNAILGSIFHIMRGIKVSDETNALDLIEKVGPG
                     GNYLLEDHTRLFARQELFTPKICYRSGNPQYTEKGFTSITDAAHEQVQIKLEQGTTRV
                     VNETIAGSISELLREVDSMVH"
     gene            574..1209
                     /locus_tag="PLL88_10900"
     CDS             574..1209
                     /locus_tag="PLL88_10900"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOJ02114.1"
                     /translation="MNDLSIIANAIYEGDMDTVVEQTKLAIEQGNSALEILENGLMAG
                     MNHVGKDFKAGDLFIPEVLIASRAMQAGMEILQPLLITQKIKSPGKIVLGTVKGDLHD
                     IGKNLVSIMLQGAGFEVIDLGVDVSKEVFLEAIEEHKPNLVGMSSLLTTTMPEMPEII
                     KAIQAAGFREQVKILVGGAPLNDTYAQAIGADGYAQNSSFAVDKAKELLGI"
     gene            complement(1283..2143)
                     /locus_tag="PLL88_10905"
     CDS             complement(1283..2143)
                     /locus_tag="PLL88_10905"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOJ02115.1"
                     /translation="MKIVTDSGTDTGSLNTEGFEIHELPLKVTLGDETYLDGQGVNQD
                     SFYAMLESGGALPKTSQPAVGEFVELYRKLAKEDKDILSIHISSGLSGTVNAAQAAAE
                     MVPEANITIVDTKTLSVGSGWQVITAAKAAAAGWTKEQIIPRLKKIAESSRTIYTLNE
                     LKYLINGGRISHMKGLLASLLNIKPLIGVDNEKGNYVQMGQSRSFKSALQGLVNLIGK
                     TIRPEEKMVAQIVHAANLEGAESLKTQVEEIFKPIWYPICQLSLVLGAHTGSSLVGIY
                     FAPASVFEGF"
     gene            complement(2478..4343)
                     /gene="typA"
                     /locus_tag="PLL88_10910"
     CDS             complement(2478..4343)
                     /gene="typA"
                     /locus_tag="PLL88_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOJ02116.1"
                     /translation="MMITERTDIRNIAIIAHVDHGKTTLVDGLLRQANTFRENQQVAE
                     RVMDSNDLERERGITILAKNTAISVYDPESRQQVKINIVDTPGHADFGGEVERVMNMV
                     DGVLLLVDAAEGPMPQTRFVLKKALEMGHQAIVVINKVDRKDAEPERALNQTFDLFID
                     LGATNEQADFPVIFASATSARAGLTPQLGPDLQPLFSTILRYIPAPKVDIDAPYRMLV
                     TNLGYDDYRGVTAMGRIYAGKIHVGQALTRITLDGQQITETAQYLYIHKGLEHIEVEE
                     AAAGEIITLAGLEGIAIGETLTDPEHPDPLPPIRVEEPTVRMTFGVNTSPFAGREGRW
                     GTSRKLRERLFAELRTNVALRVEETESTDSFLVSGRGELHLAILIETMRREGYEFQVS
                     RPEVIYHKDKDGNLLEPYEEVYIETSADTVGAVVEMLGKRHGNMKDMQENIDGSVRII
                     YIVPTRGLLGFRYQFLTATRGLGVMNTLFHGYDLASGALATRYTGSLVAWETGVTSTY
                     GLKNAEERGTLFYGAGVDVYEGMVVGEYTRPGDLTINVCKQKHLTNMRSSNKDIEIRL
                     TPPHEMSLDEAIEYLADDELLEVTPRAYRIRKRILDSEERGKLVKNAKKALEEEE"
     gene            complement(4530..>5198)
                     /locus_tag="PLL88_10915"
     CDS             complement(4530..>5198)
                     /locus_tag="PLL88_10915"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOJ02117.1"
                     /translation="ERADSYKAPLVLEGGSIHVDGEGTLMTTRECLLNANRNPDKTQT
                     EIEDLLADYLNIKKFIWLNRGVYNDETNGHVDNICCFTRPGVVALTWTDDKSDPQYEI
                     SQENFEILQSATDAQGRKLEIHKIHQPNPVLIIEEESQGVDAVEGTLPREAGDRMAAS
                     YINFYLCNGGAIVPTFDDEHDEPALRALQALLPERKVVGVPAREILLGGGNIHCITQQ
                     QPKP"
BASE COUNT         1282 a         1365 c         1203 g         1348 t
ORIGIN      
        1 tctacagcca ttgtgcagct tgcgaagaag tatggcatct gttccgacgt ctacggtctt
       61 ggaacaagcg cctttgcttt cgatccgcaa aatggctttg aaaaatcctt gaacgctctt
      121 ctgcccgttt tagcgggagc caatctgatc gctgccgccg gattaatgga agacgctctg
      181 acctccagtt atgagcagct cgtaattgat aatgccattc tcggcagcat tttccacatt
      241 atgcgtggga tcaaagtatc tgatgagacc aatgcgctcg acctcatcga aaaagtagga
      301 cccggaggaa attatttgct agaagaccat acgcgcctgt ttgcccgaca ggaattattc
      361 acccccaaga tctgctaccg ttcaggcaac ccgcaataca cagaaaaagg tttcacctcc
      421 atcaccgatg cagcccatga acaggtgcag atcaagctgg aacaggggac aacccgggtg
      481 gtcaatgaaa ctatcgccgg aagcatcagc gaactgctgc gcgaagtcga ttccatggtt
      541 cattgaaaat cacagaacag aggagaaaaa gaaatgaatg acctatcaat catcgcaaac
      601 gccatctatg aaggggatat ggatactgtt gttgaacaga ccaaactagc cattgagcag
      661 ggaaattctg cccttgaaat tctggaaaat gggttaatgg cgggcatgaa tcatgtggga
      721 aaagatttta aagcgggcga cctttttatc ccagaggtcc tcatcgcatc gcgtgccatg
      781 caggctggta tggaaatcct tcaaccactg ctgatcaccc aaaagatcaa atcgcccggc
      841 aagatcgttt tgggaacggt gaaaggcgat ctgcatgata ttgggaaaaa cctggtttcg
      901 atcatgctgc agggggcagg gttcgaagta atcgacctgg gtgtggacgt gagtaaggaa
      961 gtattcctgg aagccatcga ggagcataaa ccgaacctgg ttgggatgtc gtctctgctc
     1021 acaaccacca tgccggaaat gcccgagatc atcaaagcca tacaagcagc cggtttccgt
     1081 gaacaggtca agatcctggt gggcggtgcg ccattgaatg atacgtatgc acaagctatt
     1141 ggagcggatg ggtacgccca aaattcctcc tttgcagtgg acaaagcaaa agagctttta
     1201 gggatctgag gacaagccag agagatatca aaaaacggat tgtgcaattg cacaatccgt
     1261 tttttgatat cagccacgaa aattaaaaac cttcaaaaac agatgcgggc gcaaagtaaa
     1321 tacccaccag gctcgacccg gtatgagcgc ccagcaccag agagagttgg caaatgggat
     1381 accagatggg cttgaagatc tcctccacct gggttttcag agattcggcg ccttccaaat
     1441 tggcagcatg cacaatttga gcgaccattt tttcctcagg tctaatggtt ttcccgatca
     1501 aatttaccaa accctgtaag gcagacttga acgatcgcga ttgacccatt tgaacatagt
     1561 ttcctttttc gttatcgacc ccgatcaaag gtttaatatt caagagtgaa gccaataacc
     1621 ccttcatatg gctgatgcga ccaccgttaa tgaggtattt caattcgttc agcgtataga
     1681 tggttcggct cgattctgca attttcttta accgtggtat gatctgctcc tttgtccaac
     1741 cggctgcagc cgctttggca gccgtgatca cctgccaacc cgatcctacc gatagcgttt
     1801 tcgtatccac gattgttata tttgcctcgg gcaccatttc agccgccgct tgcgcagcat
     1861 ttaccgtgcc gctcaatcca gaggagatat ggatagagag aatgtcttta tcttcctttg
     1921 ccaatttccg gtacagttca acaaactccc ccaccgcggg ttgggaagtt ttcggcaagg
     1981 ctccgccact ctccagcatg gcataaaaac tatcctgatt gacaccctgc ccatccagat
     2041 atgtttcatc gccaagggta actttcaatg ggagttcgtg gatctcaaaa ccttctgtgt
     2101 tcaaagaacc tgtatcggta ccggaatctg tgacaatctt catctctcta tcctttctac
     2161 aatacttact cagattgccc aattctactt tgcatgcaaa aaaagatgga ttacaggaaa
     2221 tcacgaattg ccgttcactt tggtcataag cagtggttac catggtcatg cctgagcctg
     2281 tatgcaatac caggtttctc ccgctttgca gcggggcaat cacggtatta acgattttac
     2341 aggttagggg aaatttttca cccagtatct gaattcaaca tctcacccct atcttcaccc
     2401 ccgacccgaa gctgttttag aagcttccct tgttcagcag tggcacgcag agttcgaaca
     2461 cctgctgatg tatttgctca ttcttcttct tccagcgctt tctttgcgtt tttaaccaat
     2521 tttccacgtt cctcgctatc caaaatacgc ttgcgaatgc gataagcacg gggagtcact
     2581 tcgagcagtt catcatctgc cagatactcg atggcttcat ccaaactcat ctcgtgtgga
     2641 ggggtgaggc ggatctcgat atctttgttg gaggaacgca tgttggtaag gtgtttttgc
     2701 ttacaaacat tgatggtcag atcgcccggg cgggtatatt ccccgaccac catgccttcg
     2761 tatacatcca cccccgcccc gtagaaaagc gtgccgcgtt cttcggcatt cttcaatcca
     2821 taggtgctgg tcacgcctgt ttcccatgcc accagggaac cggtatagcg ggtggctagc
     2881 gctccgctag caaggtcata accatgaaaa agcgtattca tcacgcccaa tccacgggtt
     2941 gccgtcaaga actggtagcg gaaccccagc agaccgcggg tggggacaat gtaaatgatc
     3001 cgtacactgc catcgatgtt ttcctgcata tccttcatgt tgccatgccg ctttcccagc
     3061 atttcgacca cagcccctac ggtatcagca ctggtttcaa tataaacttc ctcatacggc
     3121 tccaacaggt tgccatcttt atccttatga tagatcacct cagggcgtga cacctgaaat
     3181 tcataccctt cacggcgcat ggtttcgatc aggatcgcca gatgcagttc tcctctccca
     3241 gaaacgagaa aggaatcagt gctttccgtt tcttcaacgc gcaacgccac attggtgcgc
     3301 agttctgcaa acagccgttc gcgcaatttt cgcgatgttc cccatcgccc ttctctgcca
     3361 gcaaatggag aagtgttcac gccaaaggtc atgcgcaccg tcggctcttc cactcgaatg
     3421 ggaggcaggg gatccgggtg ttcaggatcg gtcagggttt cgccaatggc aatgccttcc
     3481 aaacctgcca gggtgatgat ctcgcctgcc gcagcttctt ctacctcaat gtgttccagt
     3541 cctttatgaa tataaaggta ttgggcagtt tccgtgatct gctgaccatc caaagtgatg
     3601 cgggtcaacg cttgccccac atggatcttg ccggcataaa tgcgccccat ggcggtcaca
     3661 ccacggtagt catcgtagcc caggttggtg accaacatgc gataaggcgc atcgatatcc
     3721 actttcgggg ctgggatata gcgcaatatc gttgaaaaca agggctgcag atcaggaccc
     3781 agttgcgggg tcaagcccgc gcgcgctgaa gttgcgctgg caaagatcac cggaaagtct
     3841 gcctgttcgt tcgtggcgcc cagatcgata aaaagatcga aggtttggtt tagcgctcgt
     3901 tccggctcgg catccttgcg gtcaaccttg ttgatcacca caatcgcttg atgccccatc
     3961 tccagggctt tcttcaagac aaaacgggtt tgcggcatag gaccttcggc agcgtcaacc
     4021 agcaaaagga cgccatccac catgttcatt acgcgctcga cctcaccgcc aaaatcggca
     4081 tgtccaggag tgtcaacaat attgatcttc acctgttgcc tgctctccgg atcatacaca
     4141 gaaatggcag tgttcttcgc cagaatggtg atgccacgct cgcgttccag atcgttggaa
     4201 tccatcacgc gttccgccac ctgttggttt tcgcgaaaag tgttcgcctg ccgcagcaag
     4261 ccatccacca gggtggtttt tccatgatca acgtgcgcga taatggctat gtttcgtatg
     4321 tcagtccgtt cagtaatcat cataaccttc tctctccatt aaaaaacccg gctttgcttt
     4381 gccgaggttt ttcatgaaaa gcggcattta tcttatcaga ttgcccaaag tttgagaagg
     4441 gatgattatt tgttcccctt tcgaccactt tttcgatatg aatctttata aagaggagtg
     4501 acattgaaat cactcctctc tggaacaagt tatggcttgg gctgctgttg cgtgatgcag
     4561 tgaatatttc caccgcccaa taaaatctct ctggctggaa cacccaccac ttttctttca
     4621 ggcagcaacg cctgtaaagc ccgcaatgct ggttcatcat gttcatcgtc aaaagtagga
     4681 acgatcgctc cgccattaca gagatagaaa ttaatgtacg aagcagccat ccgatctcct
     4741 gcttctcgcg gcagcgttcc ttccaccgca tccacaccct ggctttcttc ttcgatgata
     4801 aggaccggat tgggttgatg gatcttatgg atctccagtt ttcgtccctg ggcatccgtg
     4861 gcagattgga gaatctcaaa gttttcttga gatatctcat actgcggatc agatttatca
     4921 tcggtccagg tcaaagcgac cactccgggg cgggtgaaac agcagatgtt atccacgtgc
     4981 ccattggttt catcgttata tacaccgcga ttcaaccaga tgaacttttt tatattcaaa
     5041 taatcagcaa gcaggtcttc tatttccgtt tgggttttat ctgggtttct gtttgcattc
     5101 agcagacatt cacgtgtggt cattaacgtg ccttcgccat cgacgtgaat tgatcccccc
     5161 tccagcacaa gcggagcttt gtaactatct gcccgctc
//
LOCUS       DAOSDS010000136         5149 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5733_length_5149_cov_5.685316, whole genome shotgun sequence.
ACCESSION   DAOSDS010000136 DAOSDS010000000
VERSION     DAOSDS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5733_length_5149_cov_5.685316"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="PLL88_10920"
     CDS             <1..645
                     /locus_tag="PLL88_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HOJ02118.1"
                     /translation="IDSPKEFFRSYSGTIADWSTLYPQIVNAIREVDEKTPILIGADG
                     YSSIAWLSYLIPAASINIVYDVHQYMPYDTYTHQNEDGKNRYPGTFDVDYDGKEEAFD
                     KNYLSSLLQPVRQYVQKHKVPVAINEFGVKRWVPGAANYLYDLLSLFEQEGWNYAIWE
                     WSTSYEPYGLNVDDFNYKLGIDPLNKSTLLENSILTVLRNYWSLNIIRPSNAPW"
     gene            complement(854..2020)
                     /locus_tag="PLL88_10925"
     CDS             complement(854..2020)
                     /locus_tag="PLL88_10925"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOJ02119.1"
                     /translation="MKKNILLVLLLAMLLAACSSGAASAEKDEPTLTATEPAPTDTLE
                     PTLTPTPDYPLEGRGPVDFPENVNPLTGLEVADAQTLAERVVAVKIENLPRDHRPQWG
                     LDAADLVYEYYTEFGSTRFVALYHGNVPEKVGPVRSARFFDIQVVQSYKAVFLFGSAY
                     SAVYSRMWNSDISSRLVVEGTSTYPALHRESVDGENMLFADLTQLPAALTKIGVNNSQ
                     QDLSGMFFRKTLDVSGYPATQVIVRFSTAVYNSWDYDNASGKYVRSVDQVNAASETDE
                     TYDVLTDQATGEAISADNVVILFVPYSYYVKNATEEVMEVNMLGSGDALIARDGQIFP
                     VFWQRETTASTLTLVDAEGNAFPFKPGNTWFEVVGASSSQIQNGNSWRITHAIP"
     gene            2112..2912
                     /locus_tag="PLL88_10930"
     CDS             2112..2912
                     /locus_tag="PLL88_10930"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HOJ02120.1"
                     /translation="MEKRQPWLRIVAILFFCAAFVFTVFIDAPDEFMEKIRWLGSAIC
                     HQLPSHSFHLNDQQFPLCSRCTGTYLSALLGLAFFITRGRRTGIPKKAILILFVFFFL
                     VWAIDGSNSFFYEVLQQQLLYQPSNTIRFLSGIGMGMVFSLIIMTIVNIVLWEKSESV
                     ALLKDWKEVGFLFLCESVLILFPYNKSAFLFNLAGIASVTTALVLIGLLYTILLVILT
                     HQEGTFLTMRGALPVLCFGFGIALFQFSFMANLRMRTLFSIFPYMINK"
     gene            2926..3489
                     /locus_tag="PLL88_10935"
     CDS             2926..3489
                     /locus_tag="PLL88_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HOJ02121.1"
                     /translation="MSALLQVFSAFGLSASAGLNAYIPLLITALLAKFTNLIQLEEPW
                     NALTSWWTIGLLIVLTLVETVLDKVPVVNHVNDVVQSLVRPTAGAILFASSANIITDI
                     HPVLSLALGLLISGSVNLVKTAAVRPAVAAGTAGVGNAPVSVMEDVASTGISIIAIAI
                     PIVIVVVVVLLIGWIIWRFVVRPLTSG"
     gene            complement(3496..3972)
                     /locus_tag="PLL88_10940"
     CDS             complement(3496..3972)
                     /locus_tag="PLL88_10940"
                     /inference="COORDINATES: protein motif:HMM:NF016085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HOJ02122.1"
                     /translation="MKQKITIVKRNERGIPVFHYTGEILQSTKKGLLITAIFGLPQVN
                     LRVAALLPGDLFEEYYFYDKWFNIYQVHAGNTERIKVWYCNICRPMQYLDDRIEFDDL
                     ALDLLVTPNGRQYILDRDEFCVLKINRKERRLALEGLREVQAFFKENSVPQPGMFL"
     gene            complement(3938..5029)
                     /locus_tag="PLL88_10945"
     CDS             complement(3938..5029)
                     /locus_tag="PLL88_10945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ02123.1"
                     /translation="MKETCLSIRAVSKDYEGVPLLRKIDLDLHEQETLCLLGPSGSGK
                     STLLRIIAGLERADEGNILWYGMDISDVAVEKRNFGLMFQDYALFPHMNVAENVAFGP
                     RMRGWKAEAVQERVEQTLQIVRMEKFRDRQVVDLSGGEKQRVALARTVAPNPDLLMLD
                     EPLGALDKALRDELGQVLHEILRELKIPTVYVTHDQEEAFTVGDRVAVLNFGKILQLG
                     TPEEIYSSPASLWVAGFLGFSNQVPARVIQRNPLQVSTSMGNFFVEHDSRERFAERDD
                     VILVIRPEAIRSAPKMNGQNLLSGLIRDSQFRGDGYRLTLQMENDLIFHFHDTMRRDR
                     GDRISFEIPPSAILCYPAHETEDHDRKAQ"
BASE COUNT         1306 a         1322 c         1168 g         1353 t
ORIGIN      
        1 atcgattccc caaaagaatt tttccgttct tatagtggca cgatagcaga ttggtccacc
       61 ctctatcctc aaatcgtcaa tgccattcgt gaggtggacg aaaaaacacc tattctgata
      121 ggagcagacg gatacagcag catcgcatgg ctctcatatt tgatcccggc ggcttcgatc
      181 aatatcgttt acgatgtcca ccaatatatg ccctatgaca cgtataccca ccaaaatgaa
      241 gatgggaaaa accgctatcc gggaacattt gatgtggatt acgatgggaa ggaggaagca
      301 tttgacaaaa actatctttc ctcccttttg caacctgttc gccaatacgt acaaaagcac
      361 aaagtccccg tggcaatcaa cgagttcggg gtaaagcggt gggtgcccgg tgctgccaat
      421 tacctgtatg acctcctgtc tcttttcgaa caagagggat ggaattacgc catatgggaa
      481 tggtcaacca gctacgaacc atatggactg aacgtagacg acttcaacta caaattgggc
      541 attgaccctt tgaataaaag cacgctgttg gaaaattcga tcttaactgt attgcgcaat
      601 tattggagct tgaatatcat ccgcccatcc aacgcaccct ggtgaatcca attccaacac
      661 ctctggcaga aacagaaaac cgcccagatt aacgctgagc ggttttcctg ttgtttgctg
      721 cggcatcctg ttagaggact ttgaggaggg atgccgcagc tttgggttta ccataacaca
      781 tggtgtacat cacctcctct tccttaggat agcgtctgag aggttcctat tgggttatca
      841 tatccttgac ttttcatgga atggcatgcg taatacgcca ggaatttcca ttttgaatct
      901 gcgatgaaga ggcaccaacc acttcgaacc aggtattgcc cggcttgaac gggaaggcat
      961 ttccctcagc atctaccaac gtcagtgtcg aagcggttgt ttcacgctgc caaaaaacgg
     1021 ggaagatctg cccgtcacgg gcaatcaagg catcaccgct gcccagcatg ttcacttcca
     1081 tcacctcttc cgtagcattc ttgacatagt agctgtaagg cacgaacaga atgactacat
     1141 tatctgcgct gatcgcttca ccggtagcct gatcggtcag aacgtcatag gtttcatcgg
     1201 tttcagatgc agcattcacc tgatcgaccg accgcacgta ttttccactt gcgttgtcat
     1261 aatcccaact gttgtaaacc gcagtcgaaa aacgaacgat gacctgcgtg gcaggataac
     1321 cgctcacatc caaggttttg cggaagaaca tcccgcttag atcttgttgg gaattattca
     1381 cgccgatctt ggtaagcgca gccggtaatt gggtgaggtc ggcaaagagc atattctcac
     1441 catccaccga ttcacgatga agagccgggt acgtagaggt accttccaca acaaggcgcg
     1501 aactgatgtc actgttccac atgcgagaat atactgctga ataagcgctt ccaaaaagga
     1561 agaccgcttt gtagctctgc accacctgaa tatcaaagaa acgcgcagag cgcaccggtc
     1621 caaccttctc aggaacattg ccatgataaa gagcaacaaa acgcgtgctg ccgaactcag
     1681 tatagtactc atacacaaga tcagccgcat ccaagcccca ttgcggtcgg tgatctcgag
     1741 gaaggttctc aatcttcaca gcgaccacac gctctgccag cgtctgcgca tccgccactt
     1801 ccagcccggt aagaggattc acattctccg ggaaatcgac aggtcccctt ccctctagcg
     1861 gataatcagg ggtgggtgtc agcgttggtt caagggtatc ggttggggct ggctctgtcg
     1921 cggtaagagt tggttcatcc ttttccgctg atgctgcgcc tgaggaacag gcagccagaa
     1981 gcatcgccag caataagaca agtagtatat tctttttcat attgtctcca atgtacagag
     2041 attttaccgt agaaattgaa tacttgcatc cgctccaggt atcatctata atgcattttt
     2101 caaggcaaga aatggaaaaa agacaaccct ggttgagaat cgttgcgatc ctttttttct
     2161 gtgcggcatt cgtgttcacc gtattcattg acgctcccga cgaattcatg gaaaagatcc
     2221 gttggctggg atccgcgatt tgccaccagt taccctctca ctcttttcat ttgaatgatc
     2281 agcagttccc gctttgcagt cgctgcaccg gaacttacct ttcagcgtta ttgggattgg
     2341 cattcttcat caccagggga agaagaactg gtattccgaa aaaggctatt ctgatcctgt
     2401 tcgtattttt cttccttgtt tgggcaatcg atggcagcaa ttctttcttt tacgaggtgt
     2461 tgcaacaaca gctcctgtat caaccatcca atacaatacg cttcctgagc ggtattggaa
     2521 tgggtatggt cttttctctg atcatcatga ccattgtaaa catcgtttta tgggaaaaaa
     2581 gcgaaagcgt tgctcttctc aaggattgga aggaagtcgg tttcttgttc ctgtgcgaaa
     2641 gcgtcttgat tctatttcca tacaacaaaa gtgctttcct gttcaacctg gcaggaatcg
     2701 ccagcgtcac cactgcgctg gtcttgatcg gtttgttgta caccatcttg ctggtgattc
     2761 tgacacacca agaaggcacg tttttaacca tgcggggtgc gcttcccgta ctatgctttg
     2821 gttttggcat cgcacttttt caattctcgt ttatggcaaa cttaagaatg cgaacacttt
     2881 tttcaatttt tccgtatatg attaataaat aatcaggaga atttcatgag cgcgttatta
     2941 caggtctttt cggcttttgg gttatcagcc agcgcagggt tgaatgctta tattcctttg
     3001 ctgatcacag ccctcttggc taagtttacc aacctgatcc aattagagga gccatggaac
     3061 gcattgacca gttggtggac catcggtctg ttgatcgttc tgactctggt cgagacagtt
     3121 ctggataaag ttccagtggt caatcatgtc aatgatgtgg tgcagagctt ggtgcgtcct
     3181 accgccggcg ccattttgtt tgcctccagc gcaaatatca ttaccgatat tcatcccgtg
     3241 ttatcactgg cgctgggatt gctgatctct ggcagtgtca acctggtgaa aacagccgca
     3301 gtgcgcccgg cggtcgcggc tggcacagcc ggcgtgggaa atgccccggt cagcgtcatg
     3361 gaggatgtgg cttctaccgg catatccatc atcgccattg caatccccat tgtaatcgtc
     3421 gtcgtggtgg tgctccttat cggatggatc atctggcgct ttgtggtccg cccgcttacc
     3481 agcggataga ggatcttata agaacatccc cggctggggt acggaatttt ctttgaagaa
     3541 cgcctgcacc tctctcaacc cttccagtgc caggcgtctt tcttttcgat tgatcttaag
     3601 gacacaaaat tcatccctgt ccaagatata ttgcctgcca ttgggagtta ccagcagatc
     3661 taaagccaga tcatcaaatt caatccggtc atccagatat tgcatggggc ggcagatgtt
     3721 acagtaccag actttgatac gctccgtgtt gccagcatgc acctgataga tattgaacca
     3781 tttatcgtaa aaatagtact cttcgaacaa atcacctggc aagagggcag caacccgcaa
     3841 gttgacctga gggagaccga agatggcagt tatcaacaat cccttttttg ttgattgcaa
     3901 tatctcacct gtatagtgaa aaacagggat gccacgttca ttgcgcttta cgatcgtgat
     3961 cttctgtttc atgggcagga tagcataaaa tagcagaggg gggaatttca aacgagatcc
     4021 tatccccacg gtctctacgc atggtatcgt gaaagtggaa aatgaggtcg ttttccatct
     4081 gcaacgtcaa gcggtagcca tcaccgcgga attgggagtc tcttatcaag ccgctgagca
     4141 gattttgtcc attcattttt ggagcagagc gtattgcctc cggtcggatg accagtatca
     4201 catcatccct ttccgcaaac ctttcccggc tatcatgttc aacgaagaaa ttccccatac
     4261 tggtagaaac ctgcaaagga ttccgttgaa tgaccctggc aggcacctga ttggaaaaac
     4321 cgaggaatcc agcaacccac aaggacgccg gagaagagta gatctcctcg ggagtgccca
     4381 attgcaggat ctttccaaag ttcaacactg ccactcgatc tcccactgtg aacgcctctt
     4441 cctggtcatg ggttacatat acggtcggaa ttttcaactc tctcaagatt tcatgcagca
     4501 cctgacccaa ttcatcccgc agcgctttgt ccagtgcacc cagaggttcg tccaacatta
     4561 agaggtcagg gttgggcgcg acggtgcgtg ccagagccac ccgctgtttc tctccccccg
     4621 agagatccac aacctggcga tcgcggaatt tctccatgcg gacgatctgt aaagtctgtt
     4681 ccacccgttc ttgaaccgct tccgctttcc aaccccgcat gcgcgggcca aaggcgacgt
     4741 tctctgccac attcatatgc ggaaaaagcg cataatcctg aaacatcaga ccgaaattgc
     4801 gtttttctac cgctacgtcg gaaatgtcca ttccatacca aagaatattt ccctcatccg
     4861 cccgctccaa tcctgcaata atgcgcaata gtgtgctctt gccgctgcct gaaggaccca
     4921 gcaaacacaa tgtctcttgt tcatgcaggt cgagatcaat ttttctcaac agagggacac
     4981 cttcataatc cttactcaca gcacggatag acaagcaggt ttctttcatg attttttatc
     5041 cttcaataaa agccgttcca taaaaagcat ggaaagcgca caaacagcca gcaaaataca
     5101 agccatagct atcgcctgtc cataattcaa aacacctgga agattcagg
//
LOCUS       DAOSDS010000137         5144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5741_length_5144_cov_3.963451, whole genome shotgun sequence.
ACCESSION   DAOSDS010000137 DAOSDS010000000
VERSION     DAOSDS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5741_length_5144_cov_3.963451"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="PLL88_10950"
     CDS             <1..1541
                     /locus_tag="PLL88_10950"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOJ02124.1"
                     /translation="FAVMVFDVKTAGKHAVFSSAELALLESKERPIVLASRHPRSEIS
                     AMVAPGNRCTGMMLPYTPLHLLLLEPCEGFPDVLVMTSANLSEEPIAYTNDDALARLS
                     EIADGFLMNDRDIHMRIDDSVLSEFRGEAYPVRRARGFAPQPIGLPSTAPMLLAVGAE
                     LKNAFCLSKDHYAFLSHHIGDLENVETLRSFEEAIAHYEKIFRVKPAGFACDLHPDYL
                     ASRYAAERACREDLPLFEVQHHHAHLAACLADSGCTDQQPVMGAIFDGTGLGTDGAIW
                     GGEFLIGNYSGFERKYHLAYVPQPGGDSATRTPAKMALAHLWSAGIPWDEKLAPVAFF
                     SAQERGILQKQLERSINAPPTSSMGRLFDAASALINFCQVVNYEAQAAIEMESRCVDD
                     EGGTYPFSIHDDIVDPAPLWDALLRDQQAGLSKERMASRFHNSVADLVLTVSERLRGE
                     TGIQTVVLSGGVWQNKFLLTASVSRLENAGFKVLWHRQVPTNDGGIALGQAMIAMQMF
                     NQKK"
     gene            1551..1796
                     /locus_tag="PLL88_10955"
     CDS             1551..1796
                     /locus_tag="PLL88_10955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOJ02125.1"
                     /translation="MCLGVPGKILKIYQENSIKMCEVDFDGVTMKVCLETTPQAKVGD
                     YVIVHAGFAISLLEESEALETLAMLGQIAKLGEKGLD"
     gene            1798..2883
                     /gene="hypD"
                     /locus_tag="PLL88_10960"
     CDS             1798..2883
                     /gene="hypD"
                     /locus_tag="PLL88_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOJ02126.1"
                     /translation="MTFSDTYRDPALVKQTIQNIHAAIAQPWTIMEICGGQTHALMQH
                     GFDDLLPTAVELVHGPGCPVCVTPLELIDKSIAIAQQKNVIFTSYGDMLRVPGSMADL
                     FSVRAAGGRVKVVYSPIEALQLAQKNPDWQVVFLAIGFETTAPANALSVLKAKELNLK
                     NYSMLVSQVRVPPAIHSILRDPQNRVQGFLAAGHVCSVMGYHEYEPLVKEFHLPVVVT
                     GFEPLDLALGILEAVRALEKQNHQVINAYPRAVTKEGNVKAQEIIARVYETCDQKWRG
                     IGTIPDSGWKLNAEFADFDAEHRFQVGQIQTRESEICISGLILQGRKKPVECPAFGIE
                     CTPQHPLGATMVSSEGTCAAYYRYHRR"
     gene            2886..3986
                     /gene="hypE"
                     /locus_tag="PLL88_10965"
     CDS             2886..3986
                     /gene="hypE"
                     /locus_tag="PLL88_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOJ02127.1"
                     /translation="MDKKMSEIQEKPTFGSASCPLPLRHEDQVMIGHGSGGRMTQDLI
                     KRVFLPHFDHPQLAVGNDFASLLPPSFMGKQGHFSISTDAHIVKPLFFPGGDIGRLAV
                     CGTVNDVAMAGAIPLYLTASFIIEEGLSMDVLEKVVISMAKAAQEAGVSIVAGDTKVA
                     EKGSVDRLFISTSGIGWTPHGREINGQSARPGDAILLSGTMGDHGIAVLAERGELGFS
                     TPIQSDVAPLNGLIEEVLKIAPHTHVLRDPTRGGVATTLNEIAQQSRVDMLLQEESLP
                     VKREVRAACEMLGFDPLYIANEGKLIAILPQEEAQPALRLLRSHPLSQDAALVGSVTP
                     LEGETPRVLMKTVIGGMRILDTLAGELLPRIC"
     gene            4047..4847
                     /locus_tag="PLL88_10970"
     CDS             4047..4847
                     /locus_tag="PLL88_10970"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOJ02128.1"
                     /translation="MKIDYKETPSDLLARINIHEKFGARNIDDWMIETVPLKEGMHIL
                     DVGCGAGKQCFSYFDTLHGNAQIVGGDVSEELLAKAKEENRRRGTDIVFTELDFNKPF
                     AFDDDSFDLASCSFAIYYAENIPFTIKEMHRVLKPGGYLFTTGPMPENKKTFYDIIKE
                     ATNKVIPPMPGSSRYSTEILDAVKALFAHTDLIIFENPLTFDEAEPFLVYTRASLSED
                     RKLWSSFFEGQDEFESVMHKIKAVTEKRIQKDGKIVMTKVVGGILAKK"
     gene            4852..>5144
                     /locus_tag="PLL88_10975"
     CDS             4852..>5144
                     /locus_tag="PLL88_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02129.1"
                     /translation="MAKELIFHGKRVFFLGAHPDDIELGCGGLIAHIAGKVEIFCITL
                     SDNQKNPSLSNLTAEHFHSMQSLGLDQEHIILHNFTTRRFQHERQEILEFLFDL"
BASE COUNT         1245 a         1337 c         1341 g         1221 t
ORIGIN      
        1 cgtttgcggt aatggtcttt gacgtgaaaa ccgctggaaa gcacgcggtg ttttccagcg
       61 cagaattggc gctgttggaa tccaaagaaa gacccatcgt tttggcgagc aggcatcccc
      121 ggagtgaaat ctcggcaatg gtcgctcccg gtaaccgttg caccggcatg atgttgccat
      181 acactcctct gcacctcctt ttattagagc catgcgaggg ttttcctgat gtgttggtaa
      241 tgacaagcgc gaacctttct gaagaaccca tcgcctacac gaacgatgat gcgctcgcca
      301 gattgtcaga gatcgcagat ggctttttga tgaacgatcg cgatatccat atgcgcatcg
      361 atgattctgt gctctccgaa ttcaggggag aggcttaccc ggttcgccgc gcgcgcggtt
      421 tcgccccgca acctatcggg ttgccatcca cagctcccat gcttctggcg gtgggcgccg
      481 agctcaaaaa tgctttctgc ctgagcaaag accactacgc ctttctcagt catcatattg
      541 gggatctgga aaacgtcgaa acgctgcgca gttttgaaga agccatcgcg cattacgaaa
      601 aaatcttccg ggtgaaacca gccggctttg cctgcgacct gcatcctgat taccttgcct
      661 cgcgctatgc tgcggagcgc gcctgccgtg aggatttgcc tttgttcgag gtgcagcacc
      721 accatgccca cctggcagcc tgcctcgccg attcaggctg cacggatcaa cagccggtca
      781 tgggtgccat cttcgacgga accgggctcg gcacagatgg cgctatctgg ggcggcgaat
      841 tcttgatagg aaattattca ggttttgaaa ggaaatatca cctcgcttat gttcctcaac
      901 caggcggtga ttcagctaca cgcacgccag caaaaatggc attggctcac ctttggtccg
      961 ccggcattcc atgggatgaa aagcttgctc cggtggcatt cttcagcgcg caagaaaggg
     1021 gcatcctgca aaagcagttg gaaagatcca ttaatgctcc ccccacctcc agcatgggtc
     1081 gcctgttcga tgctgcttca gcattgatca atttctgcca ggtggtgaat tatgaagccc
     1141 aggcagccat cgaaatggaa tcgcgctgtg ttgacgatga aggaggaacc taccccttct
     1201 caatccatga tgatattgta gatcccgcgc cgctttggga tgcgcttttg cgtgatcagc
     1261 aggctggtct ctcaaaggaa cgcatggctt ctcgctttca taacagcgtg gctgatttgg
     1321 tactgaccgt ctcagagagg ttgcggggtg agacgggcat ccagacggtt gtgctgagcg
     1381 gtggggtatg gcaaaataaa tttttgctga cggctagcgt ttcccgtctg gaaaatgccg
     1441 gcttcaaggt attgtggcac aggcaagttc caacgaatga tggcggcata gctctaggac
     1501 aggcaatgat cgccatgcaa atgttcaatc aaaagaaata gaggtttgct atgtgtttag
     1561 gagtgccagg aaagatcctg aaaatttatc aagagaactc aataaaaatg tgtgaggtgg
     1621 atttcgacgg tgttaccatg aaggtttgcc tcgaaaccac cccccaggca aaagttggtg
     1681 attacgtgat cgtgcatgcc ggtttcgcca ttagcctgtt agaagaaagc gaagctcttg
     1741 aaacgctggc tatgctgggt cagatcgcca agcttggtga aaaagggctg gattaacatg
     1801 accttttccg atacgtatcg tgatccggct ctggtcaagc agactattca gaatattcat
     1861 gctgcaatcg ctcaaccctg gacgatcatg gaaatctgtg gcggtcaaac gcacgccctc
     1921 atgcaacacg ggtttgacga tctgctgccc acggcggtcg aactggtgca cggtccgggc
     1981 tgcccggtat gcgtcacccc tttggagttg atcgacaaaa gcattgctat cgcccagcaa
     2041 aaaaatgtga ttttcaccag ttacggtgat atgctgcgcg ttccagggtc aatggcggat
     2101 ctcttttcag tgcgggcagc cggaggcagg gtcaaggtcg tctattcccc gatcgaggcg
     2161 ttgcagctcg cgcaaaagaa ccctgactgg caggtggtct ttctcgccat tggcttcgaa
     2221 acgaccgcac ctgccaacgc gctcagtgtc ctcaaagcca aagaattgaa cctcaagaat
     2281 tattccatgc tggtttccca ggtgcgggtt cctcccgcca ttcactccat tttgcgtgac
     2341 ccgcagaacc gcgtgcaggg ctttctggca gccgggcatg tctgctccgt aatggggtac
     2401 catgaatatg aaccgctcgt aaaggagttt catttgccgg tcgtggtcac cggcttcgaa
     2461 ccgctcgacc tggcactcgg catccttgaa gctgtgcgcg ctttggaaaa acagaaccac
     2521 caggtgatca acgcctaccc acgcgctgtc accaaagagg gcaatgtgaa agcgcaggag
     2581 atcatcgcca gggtctatga aacctgtgac cagaaatggc gcggaattgg caccatacct
     2641 gacagcggtt ggaaactaaa cgccgaattt gctgatttcg atgccgagca tcgctttcag
     2701 gtcggtcaga tccaaaccag agaatccgag atctgcattt caggtcttat cttgcagggc
     2761 agaaagaaac cggtagaatg ccccgctttt ggaatcgaat gtactccgca acatccctta
     2821 ggagcgacca tggtgtcatc cgaaggcacc tgcgccgcat attatcgcta tcacagaaga
     2881 tgagcatgga caagaaaatg agtgaaattc aagaaaaacc cacttttgga agcgcttcct
     2941 gcccccttcc attacggcat gaagatcagg tgatgatcgg gcatggcagc ggcgggcgca
     3001 tgacgcaaga tttgatcaag cgtgtcttcc tcccccactt cgatcatccc cagcttgcgg
     3061 tgggcaatga tttcgcctcc cttctacccc cttccttcat gggaaaacag ggtcatttct
     3121 caatttctac cgacgcacac atcgtgaaac cgcttttctt tccgggggga gatatcggtc
     3181 gactggcagt ctgcgggacg gtgaacgatg tggctatggc aggcgctatt cccctctatc
     3241 tgactgcttc attcatcatc gaagaagggc tatccatgga cgtgctggaa aaggtggtca
     3301 tttccatggc gaaagctgca caagaggctg gtgtgtccat cgtcgcagga gataccaagg
     3361 ttgccgaaaa aggcagcgtg gaccggcttt tcatttctac cagcggcatc ggttggaccc
     3421 cgcatggcag ggaaatcaac ggacagtccg ctcgccccgg agatgccatc ctgctttcag
     3481 gcactatggg cgatcatggc atcgctgtgc tggcagagcg cggagaatta ggtttttcta
     3541 cccccattca atccgatgtt gcccccttga atggtctgat cgaagaagtg ttgaaaatcg
     3601 ccccccatac ccatgtgctg cgtgatccta cccgtggagg ggttgccacc actttgaatg
     3661 aaatcgcgca gcaaagccgg gtagacatgc tcttgcagga agaatcgctg cccgtgaaaa
     3721 gagaagtgcg cgctgcctgt gaaatgctgg ggttcgatcc cctctacatc gccaacgagg
     3781 ggaagctgat cgccattctt ccgcaagaag aagcccaacc tgctctgcgg ttattgcgtt
     3841 ctcacccctt gagtcaagat gcagctctcg tcggaagcgt tacacccctt gaaggggaga
     3901 cgccgcgcgt tctgatgaaa acggtgatcg gtgggatgcg cattctggat acgctggcag
     3961 gagaactgct gccgcgcatc tgttgagcca acttgacaac ctaccgggag cacttatact
     4021 tattcaaaat tccacaaagg tgacttatga agatcgatta caaagagacc ccctctgatc
     4081 tgctcgcgag gatcaacatc cacgaaaaat tcggcgcccg caatattgac gattggatga
     4141 tcgaaaccgt tcctttgaaa gaagggatgc atattctgga tgtcggctgc ggtgctggga
     4201 agcagtgctt ttcatatttc gacaccctgc atggaaatgc gcaaatcgtc ggcggagatg
     4261 tctctgaaga attgctggct aaagccaaag aagagaaccg cagacgcggc accgacattg
     4321 ttttcacaga actggatttt aacaaaccat tcgcttttga tgatgattct tttgatttgg
     4381 catcttgcag ttttgccata tattacgcgg agaatatccc ttttacgatc aaggagatgc
     4441 accgcgtcct caaacccggt ggctaccttt ttacaaccgg acccatgcct gagaacaaaa
     4501 agaccttcta cgatatcatc aaagaagcta ctaataaagt catccccccc atgcctggta
     4561 gctctcgcta ctctacagag atcttggatg cggtgaaagc gctctttgct catactgatc
     4621 tcatcatctt cgaaaatcca ctcacctttg atgaagctga acctttcctc gtctataccc
     4681 gcgcttccct cagtgaagac cgcaaattgt ggagcagctt ctttgaaggg caagatgagt
     4741 tcgaatcggt catgcacaag atcaaagcgg tcacggaaaa acgcatccaa aaagatggaa
     4801 agattgtcat gaccaaagtt gtcggtggaa ttctggcgaa gaaataaaac tatggcaaaa
     4861 gaactgattt ttcatggaaa gagggtattc tttctgggcg ctcaccctga tgatatcgaa
     4921 ttgggttgcg gtggcttgat cgcgcacatc gctggaaagg tggaaatctt ttgtatcacg
     4981 ctttcagaca accagaagaa cccttcgctg tcaaacctga ctgccgagca tttccacagc
     5041 atgcaatcgc tggggttgga tcaggagcat atcattctgc acaatttcac cacccgcagg
     5101 tttcaacatg aacggcagga aatactggag tttctgttcg atct
//
LOCUS       DAOSDS010000138         5075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5861_length_5075_cov_5.992629, whole genome shotgun sequence.
ACCESSION   DAOSDS010000138 DAOSDS010000000
VERSION     DAOSDS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5075
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5861_length_5075_cov_5.992629"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLL88_10980"
     CDS             <1..629
                     /locus_tag="PLL88_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ02130.1"
                     /translation="DIGYGGLDHVLASGKNVNILVLDTGVYSNTGGQSSKATPRAAVA
                     KFAANGKDLPRKDLGLIAMTYGYIYVAQIAMGYSDLQTLRALREADAYDGPSLIIAYS
                     HCINHGYDLRKGMNQQKLAVNSGMWPIYRYNPALKEQGLNPLKLDYKEPTIPVKDFAY
                     AETRFSMLVQSNEARAEELMESAQADAIERWKYFSQLAALDFSNPAAE"
     gene            688..1698
                     /locus_tag="PLL88_10985"
     CDS             688..1698
                     /locus_tag="PLL88_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOJ02131.1"
                     /translation="MMIDLSTTYLGISLKNPLVASASVISKKLDNIKKLEDAGVSAVV
                     LYSLFEEQITQESLSLNYFLERGTESYAEAITYFPDMQSYNLEPDKYLDLIQKAKQEM
                     DIPIIGSLNGVSQGGWIKYARMIEEAGADALELNIYYLPTSAYIDATLLEKMYAGLVK
                     KIADSIDIPLAVKLNPFFTSLPSFGKDLVEYGAKGLVLFNRFYQPDLDIENMEVVPAL
                     DLSTSKDLRLPLRWIAILYSRIKADLALTSGVHNGVDIVKALMAGASVAMSASELIAN
                     GIQRARSMLDELITWMQTHEYETTDQMRGSMSQGNVAEPAAFERANYMKALNLFDADF
                     RY"
     gene            complement(1732..1980)
                     /gene="infA"
                     /locus_tag="PLL88_10990"
     CDS             complement(1732..1980)
                     /gene="infA"
                     /locus_tag="PLL88_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOJ02132.1"
                     /translation="MGKEEKIQVDGTVIEALPATQFRVRLDNGHEVLAYLSGKMRRYY
                     IRILLGDRVKVEMSPYDLTRGRITYRQKKNSPMPDEEE"
     gene            complement(2022..2213)
                     /gene="rpsU"
                     /locus_tag="PLL88_10995"
     CDS             complement(2022..2213)
                     /gene="rpsU"
                     /locus_tag="PLL88_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOJ02133.1"
                     /translation="MTKVVLRPNESQDQLLRRFRKKVIKSGVLSTIRKKRWYSSKSET
                     RRAEKKKAVRRMKRHQMTH"
     gene            complement(2408..3667)
                     /locus_tag="PLL88_11000"
     CDS             complement(2408..3667)
                     /locus_tag="PLL88_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ02134.1"
                     /translation="MKQKYSFVRLLSINSYWFGLSFLWNTLHPIVLPAILLNYVPETS
                     KNTYLGLLTFAGLLLAMLIQPLSGALSDRSGSRWGKRGPFIAIGTIFDMGVLLALAFA
                     NSLLGIFLGYVSLQIASNIAQGPMQAVIPDLVPERQKGLASGIKNFMDMAGVISASVL
                     AGILLDPASSNPLPVFLVVMGVLGFSAIITLLSIQDRQKGAASQVKEPVSLKTVFAFD
                     RKAHQSFSNLLVSRFIFLLGVYGIQTFAQYYIRDVLAVENAVAATSSLMIVIAGTLTV
                     SVLIAGWVTDRSAPERLMSIAMLVTAAGGLLLVFIQDMQTLMIVAGFIGAGLGLFLTS
                     SWAMAVKLAPSDQAGRFLGLTNLATAGASAIGKLEGPLIDVANQMAPGLFLGYRFLFL
                     FCFACSLFGYFVFKTMNKRQEQAKGEN"
     gene            complement(3720..3863)
                     /locus_tag="PLL88_11005"
     CDS             complement(3720..3863)
                     /locus_tag="PLL88_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02135.1"
                     /translation="MFYLTSRQIAFLDETAVVTQLGDAGFPMIQMIYQENDFLKPHIS
                     WLS"
     gene            complement(3913..4029)
                     /gene="rrf"
                     /locus_tag="PLL88_11010"
     rRNA            complement(3913..4029)
                     /gene="rrf"
                     /locus_tag="PLL88_11010"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(4100..>5075)
                     /locus_tag="PLL88_11015"
     rRNA            complement(4100..>5075)
                     /locus_tag="PLL88_11015"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1360 a         1353 c         1177 g         1185 t
ORIGIN      
        1 atgacatcgg ttacggcggg ctggaccacg ttttggcaag cgggaagaac gtcaacattc
       61 tcgtccttga caccggcgtt tattccaaca ccggtggtca atcatccaaa gccaccccgc
      121 gcgccgccgt tgcgaaattt gcggcgaacg gaaaagacct gccgcgcaaa gacctgggat
      181 tgatcgcgat gacctacggc tacatctatg tcgcccaaat agccatgggt tacagtgatc
      241 ttcagaccct gcgcgccctg cgtgaagcag atgcatacga tggtccttcg ctcatcattg
      301 catattcaca ctgcatcaac cacggttatg atctgcgcaa gggtatgaac cagcaaaaac
      361 tggctgtgaa ttcaggaatg tggccgatct atcggtacaa cccggctttg aaagaacagg
      421 ggttgaaccc gctcaaattg gattacaaag aaccaaccat tccggtaaaa gattttgcgt
      481 atgctgagac ccgcttcagt atgctggtgc aaagcaacga agcccgggca gaagagctga
      541 tggaaagcgc tcaagccgac gctattgaaa gatggaaata tttcagccaa ttagctgcac
      601 tggacttcag caatccggca gccgaatgat aaacaaaaga agcgtatgca tgtgcatacg
      661 cttcttttgt tctaaggatc ggaggtaatg atgatagacc tgagcacaac atatcttggc
      721 atttctttga agaatccttt ggttgcctca gcttcggtca tttcaaagaa attggacaac
      781 atcaagaaat tagaagatgc gggagtcagt gcagtcgttc tctattctct ctttgaagag
      841 cagatcaccc aagaaagcct gtctttgaac tatttcttag agcgtgggac ggaatcgtac
      901 gccgaagcga taacatactt cccggatatg caatcctaca acctggaacc cgacaagtac
      961 ctggacctga tccaaaaagc caaacaagaa atggatatcc caatcatcgg gagtttgaac
     1021 ggggtttcgc aaggaggctg gataaaatat gccaggatga tcgaggaagc aggcgccgat
     1081 gcgcttgaac tcaacatcta ttatttgcca accagcgcct atatcgatgc cactttgctc
     1141 gaaaagatgt acgccggact ggtaaaaaag atcgctgact caatcgacat tccgttagcc
     1201 gtcaagctga atccgttttt caccagcctg ccgagcttcg gaaaggattt ggtggaatac
     1261 ggagcaaaag gtttggtgct cttcaaccgt ttctaccaac ccgatctgga tatcgagaat
     1321 atggaagtgg ttcccgctct tgatctaagc acatccaaag atctgcgttt gcccttgcga
     1381 tggatcgcga ttttgtacag ccgtatcaaa gcggacctgg cattaaccag cggggtccat
     1441 aacggcgtgg atatcgtcaa ggctctgatg gctggtgcgt ccgtcgccat gagcgcatct
     1501 gagttgatcg ccaacggcat ccagcgcgcc aggagcatgc tggatgaact gatcacatgg
     1561 atgcagacgc acgaatacga gaccaccgat cagatgcgcg gcagcatgag tcaaggaaac
     1621 gtggcagaac cggctgcgtt tgagcgggca aattacatga aagcattgaa tcttttcgat
     1681 gctgatttcc gctactagta aaaatatccc ccgcaaaagc gggggatatt tttactcctc
     1741 ttcgtctggc attgggctat ttttcttttg gcgataggta atgcgaccac gggtcagatc
     1801 ataaggcgac atctcaacct tcacgcgatc acctaataag atacgaatat agtatcgacg
     1861 catttttcct gaaaggtaag cgagaacttc gtgaccatta tcaaggcgaa cgcggaattg
     1921 ggtggcaggc aaagcttcga tgacagtacc atcaacctgt atcttctctt ccttccccat
     1981 tcaaaactcc ttcctgcagt tcactgaaac gcactgatcg actagtgggt catctggtgc
     2041 cgtttcattc ggcgaactgc ttttttcttc tccgcacgac gtgtttcgct ttttgacgaa
     2101 taccagcgtt tcttgcggat agtgcttaag acaccgcttt tgatgacttt tttacgaaag
     2161 cgacgcaata attgatcctg cgattcattc ggtcgtaata ctacttttgt caatcttctc
     2221 acctcccttc gcttgaaggg taaagaaata aaaactcggc tgatagacaa agcaacagtc
     2281 gagttatctc catgattgac ttcttattcc tggaagcttc cgataaaacg cctcctcacg
     2341 ttaagataat tactgcggct tccctatcca acagccggat tgattataca tagaaccatt
     2401 ttgcttgtca attctcgccc tttgcctgct cctgcctctt gttcatggtt ttgaaaacga
     2461 aatagccaaa caaggaacaa gcaaaacaga ataagaaaag gaacctgtat cctaaaaaca
     2521 aaccgggcgc catttgattg gcgacatcga tcaagggtcc ttccaatttt cctatggcac
     2581 tggcgccagc agtggcaaga ttcgtcaacc ctaagaacct gccagcctgg tctgagggcg
     2641 ccagtttgac cgccatcgcc caactggagg tcaggaataa tcccaaccca gccccgataa
     2701 agccagccac aatcatcaat gtctgcatat cttgtataaa tacaagcaac agcccccccg
     2761 ctgccgtgac cagcatggca atgctcatca atctttcagg agcggagcga tctgtgaccc
     2821 accccgcaat gagcacgctg acggtcagcg ttccggcaat gacgatcatc aaagaactgg
     2881 tagccgctac ggcattctcc actgccaata catcgcgaat gtaatactga gcgaaggttt
     2941 gtattccgta cacccccaat aaaaaaatga agcgggagac cagcaagttt gaaaaggact
     3001 gatgggcttt tctatcgaaa gcaaaaacgg tttttaatga aacaggctct tttacctgcg
     3061 aagctgcacc cttctgcctg tcttggattg acagcaacgt aatgatcgca gaaaagccca
     3121 aaacccccat caccaccaga aaaacaggca gggggttgga gctggctgga tccaataaga
     3181 tgcctgccaa taccgaagcg ctgatcacgc cagccatgtc cataaaattc tttatcccgc
     3241 ttgccaaccc cttctgcctt tcaggaacaa gatccgggat taccgcctgc atgggtcctt
     3301 gggcaatgtt cgaagcgatc tgcagcgaaa cataccccaa gaaaataccc aacaaggagt
     3361 tggcaaaagc caaagccagg agcaccccca tatcgaaaat agttccaatg gcaatgaagg
     3421 gtccgcgctt tccccatcga gaaccggaac gatcgctgag cgccccagaa aggggctgga
     3481 taagcatggc aaggagcaag cctgcaaacg tgagcaagcc cagataagta ttcttgctgg
     3541 tttcgggcac ataattcaac agaatcgccg gcagcacaat gggatgcaag gtgttccaca
     3601 gaaaagataa tccgaaccag taggagttga tcgaaagaag tctgacgaag gaatattttt
     3661 gcttcatgag gtcacgttct ccagcacatc ggatcgttcc acttgctgcc attagtgtat
     3721 caggaaagcc aggaaatatg cggtttcaaa aaatcattct cctgatatat catttggatc
     3781 atcggaaatc cggcatcccc aagctgggtg acgaccgccg tctcatccag aaaagcgatc
     3841 tgcctggaag ttaaatagaa cacctttcga atcacagaaa ggattgatta taacaaatta
     3901 aataaaaaag ccccttggca acgacctact ctcccagggg gtcgcccccc aagtaccatc
     3961 ggcgctgacg gacttaactg ccgggttcga gatgttaccg ggtgtaccac cgtcgctcaa
     4021 atcaccaaga gacttttttt gtaaccttag aatagtatgc agacaataat ttcaaagagt
     4081 accgagtact ccgcaccacg caatgaagcc ctcgatcatt agtacggctt agcttaacac
     4141 attactgcgc ttacacacgc cgcctatcaa gcaggtagtc tacctgcgat cttactccct
     4201 tgacaggatg agggatctaa tcttgaggca agtttcccac ttagatgctt tcagcggtta
     4261 tctctgccag acgttggcta cccagcaatg ccgttggcac gacaactggc acaccagagg
     4321 tctgtccacc ccggtcctct cgtactaggg gtagctcctc tcaaatccct tgcgcccaca
     4381 gcggatagag accgacctgt ctcacgacgg tctgaaccca gctcgcgtac cgctttaacg
     4441 ggcgaacagc ccgacccttg ggaccctatc cagccccagg atgcgatgag ccgacatcga
     4501 ggtgccgagc gaagtcgtcg atgtgaactc ttggacttca ccagcctgtt atccccgggg
     4561 tagcttttat ccgataagcc acggcccttc cactcggaac cgtgggatca ctaagcccga
     4621 ctttcgtctc tgctcgacgc gttggtcttg cagtcaagct cccttatgcc tttacactct
     4681 ctggctggtt tccattcagc ctgagggaac ctttgggcgc ctccgttacc ttttaggagg
     4741 caaccgcccc agttaaactg cccacctggc acggtcctct atccggatta cggaatagag
     4801 ttagggtcta aacatgatca gggtggtatt tcaccggcgg ctccaccgag actagcgtcc
     4861 cagcttcaaa gcctcccacc tatgctacac aaaccatatc caaactcaat gccaagctgc
     4921 agtaaagctc cacggggtct ttttgtcctg ctgcgggtag gccgcatctt cacagccatt
     4981 tcaatttcac cgggtccctc gttgagacag tgctccagtt gttacaccat tcgtgcgggt
     5041 cggaacttac ccgacaagga atttcgctac cttag
//
LOCUS       DAOSDS010000139         5068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5877_length_5068_cov_3.731498, whole genome shotgun sequence.
ACCESSION   DAOSDS010000139 DAOSDS010000000
VERSION     DAOSDS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5877_length_5068_cov_3.731498"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="PLL88_11020"
     CDS             <1..988
                     /locus_tag="PLL88_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ02136.1"
                     /translation="GSVYKIVTAIGAINEGVVSPYQSLVTPYSIFITEKYAGNEVGTQ
                     REFVDWKEGGHGQANFFKGLSQSVDVYFYKISGGYQDEVKEGLGPWRMSEYAKALGYG
                     AITGIELLGEAEGLNPTPTWKRINLSENWSTGDTYIAGMGQGYVLATPLQVLVSFAIV
                     ANDGVYMKPTLIREITDSDGNVVQEFTPTQVWDITKDPLITVYDENDLPTDEKKVVEP
                     WVIDMTQQALRMTVTEGTAMSIFEGMEIPTSGKTGTAEYCDNIAREQNLCEPGNWPAH
                     AWYVGYGPSDDPEIVVVAFVYHGSEGSSVAAPIVRQVLEAYFILKEIDEEAQ"
     gene            990..1673
                     /locus_tag="PLL88_11025"
     CDS             990..1673
                     /locus_tag="PLL88_11025"
                     /inference="COORDINATES: protein motif:HMM:NF015716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOJ02137.1"
                     /translation="MSDNPKVKTREFRIKGIRDVLMVVLEEGEWENEIRDLLIYIKER
                     KDFFQSAKLAIDVGERKVKATQIADLRAGLSEQGVSLVAMFSGSVQTKNNARTFGLET
                     DPQNIRVTSGKKMRLLPDGGENAIMYNHTLRSGAKVNFSGSVMVIGDVNPGAEVIAAG
                     SVVVWGRIRGKVIAGSGGDEGAMICALEVSPLQLRIANIEAGKGLQIENPVKINCQDS
                     ELTFTAWKK"
     gene            1695..2495
                     /gene="minD"
                     /locus_tag="PLL88_11030"
     CDS             1695..2495
                     /gene="minD"
                     /locus_tag="PLL88_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOJ02138.1"
                     /translation="MAARIITITSGKGGVGKTTATANIGVGLAELGKKVTCIDSDIGL
                     RNLDVVLGLENRIVYDLVDVIEGHCKVNQAMIRDKRFENLFLIPAAQTRDKNSVTPAE
                     MVDLCESIRPEMDYILIDSPAGIEGGFKNAIAPADEAIVITNPDVSAVRDADRIIGLI
                     EAAEKGPARLILNRINPEMTKRGDMMEAEDVLELLAIKLLGIVPDDENVLISTNKGQP
                     VVLNQKSRAGQAFRNIAARITGNEVPFLNMTLKDDFMTRLSKVFNKEG"
     gene            2498..2743
                     /gene="minE"
                     /locus_tag="PLL88_11035"
     CDS             2498..2743
                     /gene="minE"
                     /locus_tag="PLL88_11035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOJ02139.1"
                     /translation="MADWLRKLSKEDSADTAKERLKLVLIHDRTNLTARVLDEMKDEL
                     IEVISRHVAIDPTEVQITVRHEGREQRLICDIPVLPK"
     gene            2751..3845
                     /gene="rodA"
                     /locus_tag="PLL88_11040"
     CDS             2751..3845
                     /gene="rodA"
                     /locus_tag="PLL88_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOJ02140.1"
                     /translation="MRRNLWRNFDFFLFGAVVFMCIFGVIMIESAIAGNEELTGYPQR
                     QSIFVVGGLVLVIIMAAIDYHLWASLARFIYVFAFVALFVIFVYGEARFGSARWIDTG
                     LILIQPSEIVKILMIMVLAEHFDRTKDEKRDFRWIARSFLLTFGVVVWILLQPNLSTS
                     IVIFVIWFAMLWVSGLPPKYLAILGVAALVLILIAFPFLEEYQQARILNFIFPDPEAS
                     YGEEYNIQQSKIAIGSGGWFGEGYGQGTQVQLRFMKVRHTDFIFSVIGEEFGFVGAMA
                     VIGLMAFIIIRCLRIAQTAADNYGSLLAFGYAAMLFFQTAVNIGVNLNLVPVSGLPFP
                     FLSYGGSSLISTILGIGLVESVALRKKTIQ"
     gene            3855..>5068
                     /gene="gltX"
                     /locus_tag="PLL88_11045"
     CDS             3855..>5068
                     /gene="gltX"
                     /locus_tag="PLL88_11045"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOJ02141.1"
                     /translation="MTSELKPVRVRFAPSPTGHLHLGSARTALYDYLIARKTGGQFIL
                     RIEDTDRKRLVEGAEEELMSNLRWLGLEWDEGPDVGGPYGPYRQSERKQIYQEHGEKL
                     VDLGYAYYCFCTPERLAKVREEKQKKKEPPHYDRLCRSIPIEEARNRIAAGEQHVLRF
                     KTPTEGTTVVKDLLRGDIAFENKTLDDYILIKSDGWALYHLAAVVDDHLMKITHVVRS
                     SEWLPTSPIHSLIHQAFGWGEPVWVHLSVLLKPSGKGKMSKREAVALSGEGYSIFIKD
                     MQGLGYLPEAVINWVALMGWSYDYRTEFFNMPDLIEKFSLEKLNPAPAATNFAKFDHF
                     NKLHIKALSDKQLAERLLPYYEAAGIHTTLAMLEKIAPIVKERIITLDDAVEMTRFLF
                     SQDVAYSKERML"
BASE COUNT         1335 a         1041 c         1416 g         1276 t
ORIGIN      
        1 gggctcggta tacaagatcg tcacggctat cggcgcgatt aatgaaggtg tggtgtcacc
       61 ttatcagagc cttgtcacac cctattcgat cttcatcaca gaaaaatatg ccggtaacga
      121 ggtgggtacc cagcgtgagt ttgtggattg gaaagaaggc ggacatggtc aggcaaattt
      181 cttcaaaggt ctttcccaat ctgtggatgt ttacttttac aagatcagcg gaggctacca
      241 ggacgaggtg aaagaagggc ttggtccgtg gcggatgagt gaatatgcca aagcactggg
      301 ttacggcgca atcaccggaa ttgaattgct gggtgaggct gaagggttaa accccacgcc
      361 aacctggaag cgcatcaatc ttagtgaaaa ttggtctaca ggcgacactt atattgcagg
      421 aatggggcag ggctacgtgc tcgcgacacc attgcaggtg ctggtttcgt ttgccattgt
      481 agccaacgat ggtgtgtata tgaaaccaac tctcatcaga gagatcaccg attcggatgg
      541 caatgtggtg caggaattca cccccaccca ggtttgggat atcaccaagg acccgttgat
      601 cacggtttat gacgaaaatg atttgcctac ggatgaaaag aaagtcgttg aaccatgggt
      661 cattgatatg acccagcagg cgttgcgcat gacggtgaca gaagggaccg ctatgagcat
      721 tttcgaaggg atggagatac caacatcggg taaaacgggg accgctgaat attgtgacaa
      781 catcgcaagg gagcagaatt tatgcgagcc tggaaactgg ccagcccacg cctggtatgt
      841 aggttatggc cccagcgatg accctgagat cgttgtggtc gcctttgtct atcatggttc
      901 cgaaggttcg agtgtggctg caccaatcgt gcgccaagtt cttgaggctt actttatctt
      961 gaaagagatt gacgaagagg cacagtaggg tgagcgacaa tccaaaggtg aaaaccaggg
     1021 aattccgcat caagggaatt cgtgatgttc tgatggtcgt tcttgaggag ggggagtggg
     1081 aaaatgagat ccgggatttg ctgatttaca tcaaagaacg gaaggatttc tttcaaagcg
     1141 cgaaactggc tatcgatgta ggtgaacgca aggtaaaagc cacgcaaatt gcggatctgc
     1201 gagccgggtt atcagagcag ggcgtcagtc tggtggctat gttttcagga tctgttcaga
     1261 caaagaacaa cgcgcgaacc tttgggctgg aaaccgaccc gcaaaatatc cgcgttactt
     1321 ccggaaaaaa gatgcggtta ttgccagatg gcggtgagaa cgccatcatg tacaaccaca
     1381 ctctgcgatc aggcgcaaag gtgaattttt ctggaagcgt tatggtgata ggagatgtga
     1441 atcctggcgc ggaggtgatt gcggcaggga gcgttgtggt atgggggcgt atacgcggaa
     1501 aagtgattgc aggttcaggt ggtgatgaag gggcgatgat ctgcgcgttg gaggtcagcc
     1561 ctctgcaatt gcgcatagcc aatattgaag cgggaaaagg actgcaaatt gagaatcctg
     1621 ttaaaatcaa ttgccaggat tcagaattga cctttacagc ctggaaaaaa taagacgttg
     1681 ttataaggag aaatatggca gctcgcatca ttacaatcac atcaggaaaa gggggtgtgg
     1741 gtaaaacaac cgccacagca aacattggag taggactggc agagttgggg aaaaaagtaa
     1801 cctgtatcga tagtgatatc gggttgcgca atttggacgt tgtcttgggt ctggagaacc
     1861 ggatcgttta tgatctggta gatgttattg aggggcattg taaagtgaac caagccatga
     1921 tcagggataa acgatttgaa aatctttttt tgatcccagc cgcgcagacg cgggataaga
     1981 attccgttac tcctgcagaa atggttgacc tttgtgagag cattcgtccg gaaatggatt
     2041 acattctgat cgattccccc gctggcattg aaggtggttt caagaatgcc attgcaccgg
     2101 ctgatgaagc catcgtgatt accaatcctg acgtatcggc tgtaagggat gctgatcgta
     2161 tcattgggtt gatcgaagcg gctgagaaag gaccagctcg acttatcttg aaccgcatca
     2221 acccagaaat gaccaaacgc ggggacatga tggaagctga ggacgtgctc gaattactgg
     2281 caatcaaatt gctggggata gtgccggatg acgaaaatgt gcttatcagc acgaacaagg
     2341 gacaaccggt tgtgttaaat caaaaatccc gcgcagggca ggcattccgg aatattgcag
     2401 ccaggatcac gggcaatgaa gtgccttttt tgaacatgac cttaaaggac gacttcatga
     2461 cgaggctgtc gaaggtattc aacaaggaag ggtagatatg gccgattggt taagaaagtt
     2521 gagcaaagaa gacagcgccg atactgccaa agaacgccta aaactggtgc tcatccatga
     2581 tcgcacaaac ctgaccgctc gggttttgga tgaaatgaaa gatgagttga tcgaagtaat
     2641 ctcccggcat gttgccattg atcccacaga agtgcagatc acggtgagac atgaaggtag
     2701 ggagcagcga ttgatctgtg atataccggt gttaccgaag taaataaatt atgcgaagaa
     2761 atctgtggcg caattttgat tttttcttgt ttggggcggt agtgttcatg tgcatttttg
     2821 gggtcattat gatcgaatct gccatagccg gaaacgagga attaaccggg tatccccaac
     2881 gacaatcgat atttgttgtt ggggggctgg tgcttgtgat catcatggct gcgatcgatt
     2941 atcatttatg ggcatcgctg gccaggttca tttacgtatt tgcttttgtc gcattgtttg
     3001 taatctttgt ttacggagaa gcccgcttcg ggtctgcgcg ctggatcgat acgggtttga
     3061 tcctcatcca accatctgag attgtgaaga tcttgatgat catggttttg gcagaacact
     3121 ttgaccggac aaaagatgag aagcgcgatt ttcggtggat tgccagaagt tttttgctga
     3181 catttggagt tgtggtgtgg atacttctgc agcccaatct atcgacttcc attgtcatct
     3241 tcgtgatctg gtttgccatg ttatgggtgt caggtctgcc gcccaaatat ctggcaattc
     3301 tgggagtggc tgcgctcgtc ttgatcctga tcgctttccc gtttttggaa gaataccagc
     3361 aagcgcgcat tctcaatttc atctttcccg atcctgaagc cagttatggt gaagaatata
     3421 atatccaaca atcaaaaatc gccattggct cagggggatg gtttggggaa ggatacgggc
     3481 agggaaccca agtgcaatta cggttcatga aggtgcggca tactgatttc attttctcag
     3541 tgataggaga ggagtttggc tttgtcggtg ccatggcggt gatcggcttg atggcgttca
     3601 ttatcatccg ctgtttacgg attgcacaga ctgccgcaga taattatggt tctctgctgg
     3661 catttggcta tgcagccatg ctgttcttcc agacggctgt caatattggg gtgaatctca
     3721 acctcgttcc tgtctcgggt ttgccatttc catttctcag ttatggagga agttctctga
     3781 tttctaccat tctgggcatt ggtttggtag aatcagtagc gttgagaaag aagaccattc
     3841 aatagaggca ttttatgacg agtgaattga aacctgtccg agttcgtttc gcgccttctc
     3901 caaccggaca tctgcatttg ggaagcgcac gaacagcatt atatgattat ctgattgcgc
     3961 ggaagacagg tggtcagttc attctgcgaa tcgaagacac cgatcgaaag cggctggtag
     4021 agggcgcaga agaagagctc atgagcaacc tgcgctggct tggtttggaa tgggatgagg
     4081 gtccagatgt gggaggtccg tatggaccgt atcgtcaatc tgagcgaaag cagatctacc
     4141 aggagcacgg cgaaaaactg gtggatctgg gctatgccta ttattgcttc tgtacgcccg
     4201 agcgattggc aaaggttcga gaggaaaaac aaaagaaaaa agaaccacct cactatgacc
     4261 gcctgtgccg gtcaatacca atcgaggaag ccagaaaccg tattgcggca ggcgaacaac
     4321 acgttttgcg ctttaagacg ccaacggaag gcaccactgt tgtcaaagac ctgctgcgtg
     4381 gggacatcgc ttttgagaac aaaacactgg atgattacat tctgatcaaa tcagatggat
     4441 gggctttata ccatctagca gcggtagtag atgaccatct catgaagatc acgcatgtgg
     4501 tgcgcagttc tgaatggctg cccacttctc ccattcacag tttgattcac caggcgtttg
     4561 gttggggtga accggtatgg gtgcaccttt cggtgttgct gaagccgagc ggaaaaggca
     4621 aaatgagcaa gcgggaagcg gtggcgttga gcggtgaggg gtattccatc tttattaaag
     4681 atatgcaggg tttaggatac ctgcctgagg cagtgatcaa ctgggtggca ctgatgggat
     4741 ggagttatga ctaccgcacc gagttcttta acatgccgga tctgattgaa aaattctcac
     4801 ttgaaaaatt gaaccctgcc ccggcagcta ccaacttcgc caaatttgat catttcaaca
     4861 aattacatat caaggcgctg agcgataaac aattggcaga acggctcttg ccgtattatg
     4921 aagccgccgg catccatact acgctcgcta tgttagaaaa gatcgcgccc atagtaaaag
     4981 agcggatcat cactctggat gacgcggtag aaatgacacg cttcttgttt tcgcaggatg
     5041 tggcttattc caaagagcgc atgttaat
//
LOCUS       DAOSDS010000140         5012 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5953_length_5012_cov_4.186807, whole genome shotgun sequence.
ACCESSION   DAOSDS010000140 DAOSDS010000000
VERSION     DAOSDS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5953_length_5012_cov_4.186807"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLL88_11050"
     CDS             <1..350
                     /locus_tag="PLL88_11050"
                     /inference="COORDINATES: protein motif:HMM:NF014300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOJ02142.1"
                     /translation="VRQAMTEMQRQIGSQGKIVMVGRDIGTVVLPDARYKFFLIASVE
                     ERARRRFEEIKASGKKADLTEIKYALEERDQKDSSRKLAPLVAAQDAKIIDTNGKTVQ
                     EVVTEMLYLIQEE"
     gene            352..1668
                     /locus_tag="PLL88_11055"
     CDS             352..1668
                     /locus_tag="PLL88_11055"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOJ02143.1"
                     /translation="MCDTSVVLATASKNGEAFFAKNSDRSPNEAQYLTIFPAKDHNKS
                     ALVRCTYVEIPQARHTFSVLLSKPYWMWGAEMGANECGVVIGNEAIFSKVPAGKAPGL
                     IGMDYLRLALERSATALEALRTITDLLQNYGQSGNCSAEHELYYHNSYLIMDGKEAYV
                     LETIDRMWIAKKVKDFYAISNGLSITTEWDFISDGLIDYAVEKKWCRDKKRFNFKESF
                     SDTIYSKFSQGGERRTCVMKTLEKNRGNIDLQLMKTVLRSHDLNPQKSFPDRSFVAWN
                     VCMHAGPGPIRDSQTTGSLITQYSKNAHTFWVTGTAAPCISVFKPVWISSGLPDIGPE
                     PNGVYDTNCLWWQHEVLHRRVLKDYHTRHAFLKPQIEKVEEDIDKLIADGTQGKTRLT
                     EKAFRLEKTRLPEWILQVDTISGGHKNARYYERYWRKKNKKVNIPV"
     gene            complement(1738..1926)
                     /locus_tag="PLL88_11060"
     CDS             complement(1738..1926)
                     /locus_tag="PLL88_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02144.1"
                     /translation="MKREKINAEERRTEDREKSVRKPVQRILFDQLEALGETIDKKNQ
                     HRDPETEQNPVHPGAGEI"
     gene            2026..4878
                     /locus_tag="PLL88_11065"
     CDS             2026..4878
                     /locus_tag="PLL88_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ02145.1"
                     /translation="MLSIFLAMTGSMIFSGFWGHNLFYVLLIVSAALLFNYFLSAKDG
                     LFSQFNNHPLVLISLVVLCIFPLFYIERFASFSGEVPLRFDLCFHDGWIFYLSRAFEL
                     MIAGIIAEILAKRAGRMEKSSAPSKTPLGNRTIAVHLISFFYLTLVILTLLWNITRQH
                     ALNEWKSNIKERIGIVDTSVVSTFTANAIRIDQLSKEGLIHGNSNDIREQIKPLFQPV
                     QNLDAFYLFDRQGELVFSYPYVAEKGLEISDIEVHAFQDVLEKATIQAAFSSYASDVH
                     MSILYPITGEDKTIQRVVIARVNLQSNPVFLPLATLMQSYQQDGLQVAFINTQFNAKT
                     AWSDPVQNDLEETATSLYAGMGLEGWGIEMSLTKKAFLIDFFYTFLPYLTTMLVCVAA
                     VSGLYFLRWERMEKALINLTARFSGENAASDQPERNMAFPRSVLVFLEVLKTVFKKMD
                     RRHQETQTLLDLWNNYDSHTEFQSIVRRILSSFAEDDSLFVEILLEEKEGELLSEKYV
                     LSLEKDTEDFSYLDEQIQAVSQEQNQLVIGNTTRFHQLVRALGKPFPQALIISKFPID
                     SERTGIFVHAFRSFHEFSKDEIDAFNKKAESFYNQIAAILRLQRWLIEKRIFSNLFDS
                     LNFPLFLFINQELIYGNKAAHAFLKIDETEVHTHIEKRVQENEIYNVMLRNSSQERAV
                     ITKELPSGEKYEINILNSNDPTAGNISVLLLKDITSEKKRHELTHDFVSMLSHDLRSP
                     ITVLQGYSKMLPMVGELNPTQQDYLEKIKGGLETMLALVEGILTEDRIEKGVVISPAE
                     IDLGAVIQEVIAQFESLANQKRVKISVQGATSGVFIKGDAILLKQAFYNVIHNAIKFS
                     GLDGSVEVKLLENDENVVLEVRDSGPGIAALDIPFIFEKYYYPKTGDLTAEKTGGSGL
                     YITKFIIDAHRGSINVDSELGKSTAIRIILPKKISV"
BASE COUNT         1379 a         1054 c         1225 g         1354 t
ORIGIN      
        1 gggtgcgcca ggcgatgacc gagatgcaac gccagattgg ttctcaggga aagatcgtga
       61 tggtcggtag ggatataggg acggtggtgt tgccggatgc cagatataag ttctttctga
      121 tcgcttcggt tgaggagcgt gccagaaggc ggtttgaaga gatcaaagcc agtgggaaaa
      181 aagccgattt gactgaaatt aaatatgcct tggaagagcg cgatcaaaag gattcttcga
      241 gaaagcttgc cccactggtg gcagcacagg atgcaaagat catagatacg aatgggaaaa
      301 cagtacaaga agttgtcacg gaaatgctat atttaatcca ggaggaatga tatgtgcgat
      361 accagcgtag tccttgccac cgcgtcaaag aatggagaag cgttctttgc caagaattca
      421 gatcgtagtc ccaatgaggc gcaatacctt acgattttcc ctgcgaaaga tcacaataag
      481 agtgcattgg tacgctgcac gtatgttgag ataccgcagg cgcgccatac tttttcggtg
      541 ttgctttcga agccttattg gatgtggggt gcagaaatgg gggcgaatga gtgcggggtg
      601 gtgattggca atgaagccat tttcagtaaa gtcccggcgg gtaaggctcc tggcttgatt
      661 ggaatggact atttgcggtt ggcattagag cgctctgcta cagcccttga ggcattgcgt
      721 acgatcaccg accttttgca gaattacggt caaagcggaa actgcagcgc agagcatgag
      781 ctttactacc ataatagtta tctgatcatg gatggaaaag aggcttatgt gttggagacc
      841 attgatcgca tgtggattgc caagaaagtg aaagattttt atgccatctc caatggattg
      901 agtatcacaa cagagtggga tttcatctct gacggactca tcgattatgc agtggagaag
      961 aagtggtgtc gcgataaaaa aaggttcaac tttaaagaat ctttttccga tacaatctac
     1021 tcaaaattca gccagggtgg tgaacgcaga acgtgcgtaa tgaaaaccct tgagaaaaac
     1081 agagggaaca tcgatctcca attgatgaaa actgttttgc gctcccatga cctgaatcca
     1141 cagaaatctt ttcctgatcg ttcttttgtc gcctggaacg tttgtatgca cgctggtccc
     1201 ggacccatcc gtgatagcca gacgacgggg tcattgataa cccaatacag caagaatgca
     1261 catacattct gggtgaccgg aactgctgcg ccgtgtatat ccgtgtttaa gccggtatgg
     1321 atatcgtccg gtttgccaga tattggaccc gaaccaaacg gcgtgtatga cacgaattgc
     1381 ctatggtggc agcacgaggt tttgcatcgt cgtgtgttga aggattacca tacccggcac
     1441 gcattcttga agccgcagat cgaaaaagtg gaagaggaca tcgataaact cattgctgat
     1501 ggaacacaag ggaaaaccag gctcactgag aaagctttcc ggttggaaaa aacgcgtctt
     1561 ccggaatgga tcttgcaggt cgatacgatc agcggtggtc ataaaaatgc gcgttattat
     1621 gaaagatatt ggcggaaaaa gaataaaaaa gtgaacatcc cggtatagaa ttaaagaaat
     1681 aaaaataatg atccggtgat ggaagcattt ttacaacaaa tatccacgaa cttttttcta
     1741 aatctcccca gcacctgggt ggactggatt ttgctctgtc tctggatctc tgtgttggtt
     1801 tttcttatct atcgtttctc ccaacgcttc caactgatcg aaaagaatcc gctggactgg
     1861 ctttcttaca cttttctcac gatcctctgt tcgtcgctct tccgcattga tcttttccct
     1921 tttcatgtac aggtttttcc ttccaacgcg taccctggga gcgaaatttt tttaatcctt
     1981 tttcaagcta tcccctgggt ggcaactgct ctatttggga aaaggatgct gagcatcttt
     2041 cttgctatga ccggaagcat gatcttcagc ggtttttggg gacataatct gttctatgtc
     2101 ttactcatcg tatccgctgc gcttttgttc aattatttcc tttcagcaaa agatggtctc
     2161 ttctctcaat tcaataatca tccactggtg ctgatcagcc ttgtagtctt gtgcattttc
     2221 ccgctgttct atatagaacg ttttgccagc tttagcgggg aagtaccgct gcgtttcgat
     2281 ctctgttttc atgatggttg gatcttctat ctgagccgag cttttgaatt aatgatcgca
     2341 gggatcatcg ctgagatttt agcgaaaaga gccggacgca tggaaaaatc cagcgctcct
     2401 tccaagactc ccctgggaaa tcgaacgatc gctgttcatc tcatcagttt tttctatctc
     2461 acgttggtca tactgaccct tttatggaac ataacacgcc agcacgcgtt gaacgaatgg
     2521 aagagcaaca tcaaagagcg gataggcatt gtagatacgt cggtcgtatc aactttcacc
     2581 gccaatgcca tccgcatcga tcaactgtcc aaagaaggat tgatccatgg aaacagcaat
     2641 gatattcgtg agcagatcaa accgcttttc caacccgtgc aaaatctgga tgctttctac
     2701 ctttttgata ggcaaggcga actcgttttc tcttaccctt acgttgctga gaagggatta
     2761 gagatttctg atatagaggt gcatgcattt caagatgtgc ttgagaaggc taccatccag
     2821 gcagctttct cttcgtatgc ttcagatgtt cacatgagca ttctttaccc tattacggga
     2881 gaggataaaa cgatccagag agttgtgatt gcccgcgtga atttgcagag caatccggta
     2941 tttcttcctt tggcgaccct gatgcaatct tatcagcagg atggattgca agtcgcgttc
     3001 atcaatacgc agttcaacgc gaaaactgcg tggtcggatc cggttcaaaa tgatttagaa
     3061 gaaactgcta cctcgctcta tgcaggcatg ggattggaag ggtggggcat cgaaatgagc
     3121 ttaacgaaaa aagcgtttct gatcgatttc ttctacacgt ttctccctta cttaacgacc
     3181 atgttggttt gtgttgctgc cgtcagtgga ctttattttc tccgttggga aaggatggag
     3241 aaagcgctta tcaacctgac cgcccgtttt tctggagaaa acgccgcgtc cgaccaaccc
     3301 gaacggaata tggcttttcc gagaagcgtc ctggtttttc tggaggtctt gaaaactgtt
     3361 tttaaaaaga tggatcgaag gcatcaagaa acccagactt tgctcgactt gtggaataac
     3421 tacgatagcc acacagaatt ccaatccatt gtcaggagaa tcctgtccag ctttgctgag
     3481 gatgattcat tatttgtgga aattctgcta gaagaaaaag agggagagtt gctctctgaa
     3541 aaatatgtgc tttccttaga aaaagacacg gaggatttct catacctgga tgaacagatt
     3601 caggcggtga gccaggaaca gaaccaattg gtaatcggca ataccacacg tttccatcag
     3661 ttggtgcgcg cgcttgggaa gccttttcca caagccttga tcatttctaa atttccgatc
     3721 gattcagaac ggacaggtat tttcgttcac gcatttcggt ctttccatga attctcgaaa
     3781 gatgaaatcg atgctttcaa taagaaagca gagtctttct ataaccagat cgctgctata
     3841 ttgcgtttac agcgctggct catagaaaag aggatcttct ccaatctatt cgacagtctg
     3901 aactttccac tcttcctctt cataaatcag gagttgatat acgggaataa agctgcccat
     3961 gctttcttga agatcgatga aacagaagtg cacacgcata ttgaaaaacg tgtgcaggag
     4021 aacgagattt acaatgtcat gctgcgcaat agctcgcagg aaagagcggt tataacgaaa
     4081 gaactgcctt ccggagaaaa gtacgaaatc aatatcctga attcgaacga tccgacagca
     4141 gggaatatct ccgttctgtt gcttaaggat atcaccagcg aaaagaagcg gcatgagctg
     4201 acccatgatt ttgtcagcat gttgagtcat gatctccgct ctccgatcac cgtcttgcag
     4261 ggatattcga aaatgttgcc catggtgggc gagttgaatc ctacccaaca ggattattta
     4321 gaaaaaatta aaggtggatt agaaactatg ttagcgctgg tggagggaat attaactgaa
     4381 gaccgcatcg aaaaaggagt ggtgatttca ccggcagaga tcgacctggg cgctgtgatt
     4441 caagaggtga tcgcacagtt tgagtcgtta gccaatcaaa aacgggtgaa aatctcggtg
     4501 cagggggcga catccggagt attcatcaag ggtgatgcga ttttgcttaa acaagctttt
     4561 tataatgtga tccacaacgc catcaaattc tcaggattgg atggctcggt tgaagtgaag
     4621 ttgctggaga atgatgaaaa cgtggtgctg gaggtgcgcg attcgggtcc gggaattgct
     4681 gcattggata tcccgtttat ttttgaaaaa tattactatc ccaaaaccgg tgatctcact
     4741 gcggagaaaa caggtggttc agggttgtat attacaaaat tcatcattga tgcgcatagg
     4801 gggagtatca acgtagacag cgagttgggc aaaagcaccg cgatacggat catattgcca
     4861 aagaagattt cggtctgaca acccacgctc tcgaatgtaa catttttgca cgttttttct
     4921 agcatgtcaa ggctaagtag aaagatccta aaggtgagcc aaatagaaag aacctaaggg
     4981 tctaccagaa ggacaggagt accgtcgagt tt
//
LOCUS       DAOSDS010000141         4984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_5991_length_4984_cov_4.899168, whole genome shotgun sequence.
ACCESSION   DAOSDS010000141 DAOSDS010000000
VERSION     DAOSDS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5991_length_4984_cov_4.899168"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="PLL88_11070"
     CDS             complement(<1..668)
                     /locus_tag="PLL88_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02146.1"
                     /translation="MNHTIIGLMKVSSSLLFFLIIPFFLSCCYKPELVTYAESVEQNQ
                     TPTQPLLTPLADLENSLSEVRNKIDLWTRATPMLRGANIWQAVVIPAVDGTFKGNGSV
                     GPPFTQEDFDLLASLGANYVVISAPGLFTENPPYQVDEEVQRNLDRMLEMIEKADMFA
                     TIAFRTGPGKAEWSLCCSDTHDYDGYFNDAVWSDPQAQAAWVAMWRYTAERYRGNRIV
                     AGYEL"
     gene            755..2245
                     /locus_tag="PLL88_11075"
     CDS             755..2245
                     /locus_tag="PLL88_11075"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HOJ02147.1"
                     /translation="MKNHQLVFIGLLVAGLLLAGCKEGRTIQSDHPEETGALDIFQAG
                     LIESERTHLEQFDTATQYTLHLTFHEPFTEIVGREQVIYFNNEDVPFEEIYLRMFPNN
                     SGDTMVVTQILVDGEEIPVQTAYKNSALRVALPALLQPGESVVLDLVYTIQIPTDFGG
                     NYGLFSYSDGILALDQVYAIIPVYDDEEWNVEEPAPNGDMIYTDPAFFTVTVEAPADL
                     VIAATGVLVEEHQTDGGKAQTFVAGPVRDFYLAASKQYASVSEQVGETLVTSYFPEGY
                     EEGGEFVLDVAVNALRSFNERLGVYPYTELDLVGTPMQAAGMEYSGIVAMSTQFYDLE
                     ATFSGVPAFTYLESATAHEVAHEWFFNMIMSDQIDEPWIDEGMAQFLTSLYYEDRYGV
                     AAAERYQESWSSRWSRVDFKPMPIGLPAQDYTEAEYGAIIYGRAPLFIQSLRDNMGED
                     AFMEFLKAAFSSFKWEILNTGDFQALAGETCGCDLQARFSNWVFDK"
     gene            2499..3761
                     /locus_tag="PLL88_11080"
     CDS             2499..3761
                     /locus_tag="PLL88_11080"
                     /inference="COORDINATES: protein motif:HMM:NF018234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ02148.1"
                     /translation="MGTVNRKRDTQHWIMDYLIKQTGRYQHYAYDVKTFPPDVKSYKM
                     IPRVMERYGRHREEMAKAAEAAGHYETAGELYFLASDKYKLAQHAIYEDDNQEKIYLH
                     GKVVECFMKAAAYAPNPIELVEIPFEDNYIQGVLHLVPGGKKAPTVIFAPGMDMTKES
                     TLNPFHHPFVTRGMNFLALDGPGIGTSNIRKIHVTPDNYGKAGTAAIDYLSTRSEVDI
                     EKIGVSGYSFGSYWGMQMAAADKRIKAVGTAAACYGPMTSIFEEETPHFKQVFMYMAG
                     YEDEDEFDKMASQMRLDDVAGKVTCSCLIVSGEYDPLAPLETVINVFDLVSGPKELWV
                     VEDEFHTPRHGESFGGADFYGILADWIKDALNGKYTAGWDKRIMIRKMSEGPYSKEAP
                     YVALPERASVKYGGHTEQQLGPGGIRKK"
     gene            complement(4001..4074)
                     /locus_tag="PLL88_11085"
     tRNA            complement(4001..4074)
                     /locus_tag="PLL88_11085"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4038..4040),aa:Arg,seq:cct)
     gene            4192..>4984
                     /locus_tag="PLL88_11090"
     CDS             4192..>4984
                     /locus_tag="PLL88_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ02149.1"
                     /translation="MENAVEVKGLKKFYKDVKAVNGVDFTIQSGEIFSLLGPNGAGKT
                     TIINVLTGLFPPNAGDALIFGHSIQKEPLKAKALMGVVPQEIALYEDLSARENLVFWG
                     KLYGLRGKQLQLRVDEVLELIGLRDRQRQRVSKYSGGMKRRVNIGIALLHLPKLVIMD
                     EPTVGIDPQSRRHILDNVLQLNSQGMTVLYTTHYMEEAQELSTRIAIMDQGKVVASGR
                     HADLVKLVGEQDRVELTLSQDGEKVLKAWKKVLGVSKIDQDGEKIT"
BASE COUNT         1306 a         1040 c         1374 g         1264 t
ORIGIN      
        1 agttcgtacc ccgctacaat acgatttccg cggtaacgtt cagcagtgta ccgccacatc
       61 gctacccacg cggcttgggc ttgaggatcg ctccagactg cgtcgttgaa gtagccgtca
      121 tagtcgtgcg tatccgaaca gcataggctc cattctgctt ttccaggacc ggtgcgaaaa
      181 gcgatcgtag cgaacatatc cgctttctcg atcatttcca gcatgcgatc cagatttcgc
      241 tgcacctctt catccacttg atagggaggg ttctcggtga acaagcccgg tgcagagatg
      301 accacataat tggcgcccag ggaagccagc aggtcaaaat cttcctgagt gaaaggtggt
      361 ccaacgctcc cgttgccctt gaaggtgcca tccactgcgg gaatcaccac tgcctgccag
      421 atgtttgcgc cgcgcagcat gggtgttgcc cttgtccaca ggtcaatttt gttcctgacc
      481 tcggaaagcg aattttccag atcggcaagg ggtgtcagca gaggttgagt tggcgtttgg
      541 ttttgttcga cggattctgc ataggtgacc aattccggtt tgtagcagca ggacaggaag
      601 aaggggatga ttaaaaagaa taacaatgag cttgatactt tcatcagccc tattatagtg
      661 tgattcattc cttttctttg agtcagccgt ctgcttcacg ccaaagcagc gtgaattagg
      721 taaaatgata aaaagtatga tcgcggagaa cgcaatgaaa aatcaccaac tcgtcttcat
      781 tggattgctt gttgcaggct tgttgttggc tggatgtaaa gaagggcgga cgatccaaag
      841 tgatcatcct gaagaaacgg gtgcgctgga tatttttcaa gcgggattga tcgagagtga
      901 acgaacgcac ttggagcaat ttgatacagc gacgcaatat actctgcatc ttactttcca
      961 tgaaccattc acggagatcg ttggtcggga gcaggttatt tactttaaca acgaagatgt
     1021 accgttcgaa gaaatctatc tgcgcatgtt ccccaataac tctggagata ccatggtcgt
     1081 gactcaaatt cttgtggacg gtgaagaaat accggttcaa accgcatata agaacagcgc
     1141 tttacgggta gcactgcctg cgctcctcca accgggggaa agcgttgtcc tcgatctcgt
     1201 ttatacgata cagattccga ccgattttgg cggaaattat ggtctgttca gctatagcga
     1261 cggcattttg gctctcgacc aggtttatgc catcatccca gtttatgatg atgaagagtg
     1321 gaacgtggaa gagccagcgc cgaatggcga tatgatctac accgatccgg cttttttcac
     1381 tgtgaccgtt gaagccccgg cagacctggt aatcgcagcc accggtgtgc tggtggaaga
     1441 gcaccagacg gatgggggaa aagcccagac ctttgtggct ggtcctgtgc gcgattttta
     1501 cctggctgcc agcaaacaat atgcatcggt gagtgaacag gtcggcgaaa ccctggttac
     1561 cagttatttt ccggagggtt acgaagaagg tggagaattc gttttggatg tggcagtgaa
     1621 tgcgttacga tctttcaatg aaagactggg cgtgtatccg tatactgaac tcgatctggt
     1681 gggtacgccc atgcaagctg ccgggatgga atattctggt attgtggcga tgagtaccca
     1741 attctatgat cttgaagcga ccttttccgg cgtccccgct tttacgtatc ttgaatctgc
     1801 cactgcgcac gaggttgctc acgagtggtt cttcaatatg atcatgagcg atcaaatcga
     1861 tgagccgtgg attgatgagg gcatggcaca atttctcacc tctctttatt atgaggatag
     1921 atatggggtc gctgcggctg aaaggtatca ggaatcgtgg agttctcgat ggagtcgggt
     1981 tgatttcaag cccatgccaa tcggtttgcc ggcgcaggat tatacggaag cggaatatgg
     2041 agccatcata tacggacgcg ctccgctctt catccaaagt ttgcgcgata acatgggtga
     2101 ggacgccttc atggaatttt tgaaagccgc tttttcttcg ttcaaatggg agattcttaa
     2161 tacgggtgat ttccaggctc tggcgggaga aacatgtggc tgcgatttgc aagcgcgctt
     2221 ttcgaattgg gtcttcgaca agtagatcgt agaaaatatc tgatgatcgc tgttctcatg
     2281 agattgcaat gccatcctat ggctgttttg taggaatggg ggatgatgta ttgcacatca
     2341 tttgcagacg tttcttgaaa ttatctgcaa atcgctgttc gcgcgtggga aaatctttga
     2401 ggcagagcat taaatcagga aagattcaca gaattgaaaa aaaggaaatg tcttcaaatg
     2461 tataatggga ctattcgaaa tcagtgaaag gagattttat gggtacagta aatcgaaagc
     2521 gcgatacgca gcattggata atggattact tgatcaaaca aaccgggaga tatcagcact
     2581 atgcgtatga cgtaaagacc tttcctcctg atgtgaaatc ttacaagatg attccacggg
     2641 tgatggaacg gtatggcagg catcgggaag aaatggcaaa ggctgccgaa gctgccgggc
     2701 attatgaaac agcgggagaa ttgtattttc ttgccagcga taaatacaag ctggcgcaac
     2761 atgctatcta tgaggatgac aatcaggaaa agatctatct gcatgggaaa gtggtggagt
     2821 gcttcatgaa agcagctgcc tatgcgccca atccgatcga attggtcgag atcccatttg
     2881 aagataacta cattcagggc gttttgcatt tggtgccggg agggaagaaa gctcccacgg
     2941 tcattttcgc ccctgggatg gatatgacca aagaatccac gcttaatcct ttccatcatc
     3001 ctttcgtcac acgaggaatg aattttctgg ctcttgatgg accggggata ggcaccagca
     3061 acatccgcaa aatccacgtt accccggata attatggaaa agccggaacg gctgccatag
     3121 attatctgag cacaagatcc gaagtggata tagagaagat tggagtgtcg ggatattcct
     3181 ttggctctta ttgggggatg cagatggcag ctgctgataa aagaatcaag gcggtgggaa
     3241 ctgccgctgc ttgctatggt cctatgactt cgatctttga agaagagacc cctcatttca
     3301 agcaagtttt catgtacatg gctggatacg aggatgagga tgaatttgac aaaatggctt
     3361 ctcagatgcg cctggacgat gtcgccggca aagtaacctg ctcctgcctt atcgtgagcg
     3421 gtgaatacga tccgctggcg cccctggaaa ctgtgattaa tgtgtttgat ctggtttctg
     3481 gcccaaagga actttgggtt gtcgaggatg agtttcatac cccgcgccat ggtgagtctt
     3541 tcggcggagc ggatttttac ggcatactgg cagattggat taaggatgct ttgaacggta
     3601 aatatacggc aggttgggat aaacgtatca tgattcgcaa gatgagcgaa ggaccctatt
     3661 cgaaagaagc gccctatgta gcccttccgg aacgggccag tgttaagtat ggtggacata
     3721 cagaacagca actgggtccg ggcggcataa ggaaaaagta ggttcggcat agaatcttgt
     3781 gcttgtgtcg aaagagaggt tgtccgggta gatgacctct cttattatgt acaggaggga
     3841 ggctgacggt tgtctgatgg acaacccata ggaaaggaag gatgacaaag gttctaccgt
     3901 tattcaaggt tttggatgtc accaaaggaa gataagcaac gtgattttca gagaccgttg
     3961 tctgagaatt taaaaatcca gccactggct ggatttttaa tgccccagga gggactcgaa
     4021 cccccaacct aggcgttagg agtgcctcgc tctctccatt tgagccactg gggcaggtgt
     4081 gattgaatta tagcattggc aaatgaggct ggcaagaaag ggtgtgacac ctgtgggttt
     4141 ttcttacgta tatagtgaag aagatatctt tgagttggag ggtgagccac gatggaaaat
     4201 gcagttgaag taaaaggatt gaaaaagttt tataaagatg tgaaagcggt gaacggagtg
     4261 gattttacta tccagagtgg ggaaattttc agtttgctgg gtcccaacgg cgcaggcaaa
     4321 acgaccatca tcaatgtgtt gaccggactt tttcctccca atgcaggaga tgcgttgatc
     4381 tttggtcatt ctatccaaaa agaaccgcta aaagcgaaag cgttgatggg ggtggtgccg
     4441 caggagatcg ccctgtacga agacctttct gccagagaga atctggtgtt ctggggaaaa
     4501 ttgtatggct tgcggggaaa gcagttgcaa ctgcgggtgg atgaagtgct cgagttgatc
     4561 ggattgcgcg accgccagcg ccaacgagtc tccaaatatt ccggtgggat gaaacgcaga
     4621 gtaaatatcg ggatcgcgct gctgcatttg cccaagttgg tgatcatgga tgaacccacg
     4681 gtaggcatcg atccacaaag ccgcaggcac attctggata atgtgctgca attgaacagc
     4741 cagggcatga ctgtgctgta taccacgcac tacatggaag aagcccagga attatctacc
     4801 cgtattgcca tcatggatca gggtaaagtc gtggcaagtg gaaggcatgc agatttggtg
     4861 aagttggtag gcgaacagga tcgcgtagag ttgaccctca gtcaggatgg agaaaaagta
     4921 ttaaaggcat ggaagaaagt gctgggtgtt tcaaagatcg atcaggatgg tgaaaaaatc
     4981 actt
//
LOCUS       DAOSDS010000142         4826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_6230_length_4826_cov_5.046741, whole genome shotgun sequence.
ACCESSION   DAOSDS010000142 DAOSDS010000000
VERSION     DAOSDS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6230_length_4826_cov_5.046741"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLL88_11095"
     CDS             <1..303
                     /locus_tag="PLL88_11095"
                     /inference="COORDINATES: protein motif:HMM:NF023746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOJ02150.1"
                     /translation="IKETAHPDVNLIFGAVIDPNMGDECRITVIATGFDQAGMRSRLR
                     HFSDLNEKKTDENRFSARPVKDNPESIRTQYDFQPRVYNTEDIEKIPAFIRNREND"
     gene            614..1078
                     /gene="nrdR"
                     /locus_tag="PLL88_11100"
     CDS             614..1078
                     /gene="nrdR"
                     /locus_tag="PLL88_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOJ02151.1"
                     /translation="MRCPHCESENTRVIDTTHDAKGGIRRRRICEDCDQRFSTYERPV
                     LSTPLIIKRDNTREEFDKEKLLQGIRLACAKRPVPAEKIERLAGEIEAKLQHSTKLEI
                     PSKVIGDLVIEGLRKLDDVAYIRFASVYLPLGDLNALRDEIDRLLEHKGENR"
     gene            1075..3714
                     /locus_tag="PLL88_11105"
     CDS             1075..3714
                     /locus_tag="PLL88_11105"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOJ02152.1"
                     /translation="MTSNSASQPPKNDRPFVEMPHGLPKVEFSENSYQVFTKRYVRKG
                     EDGQLTETPEETFWRVTWHIAAVEDKSEEEIIETASQFYNLMASMKFLPNSPTFTGAG
                     TPLGQLAACFVLPISDDMGKKPSGIFQTLRDAALIQQTGGGNGFSFSSLRPSGALVKS
                     SAGKATGPIGFMRVFDHAFGEVAQGGTRRGANMAVLRVDHPDIESFITCKTRENAITN
                     FNISVGITDAFMKAVEEDAMWDLRFPDVNHPLYRDFEGTFEDAERAGIPLIIHKTVRA
                     RDLFNLIAFQAHHNGEPGMLFMDSANRSNPIPNLYSLEATNPCGEQWLGPYENCCLGS
                     INLSKHYLADTGVDWEALRSSIQLAVRFLDDVVDANKYVPSVPQLRENALRARRIGLG
                     FMGLADLMYLCGIRYGSEEGQEFAAQIMEFIRYHAMLTSIELAKEFSPFPAIHDSIYN
                     PEKMTWQPPQSIVAYTHNWGRPDLDWNKVIKDIRTYGIRNAALTTIAPTGTISTVAGC
                     EGYGCEPVFALAYVRHVDDGGKDLELTYLSPIMQAALDQWDLTPEQRETIVQEILEEG
                     SCQAIDQLPEQIRNVFVVSQDISGEEHVRMQAVLQAFVDNSISKTINFPSNARENDIA
                     AAFLLAWKLGCKGITAYVTGSREQVVLETAATRNQKQSVEMREMVQPSNDPLSPVWLT
                     ENDYDQPNLWSEAKKPRPRYLSGMTYGIKTPVGKTFITINESGFNQPFEVFINTAKAG
                     SDVAADSEALARLISYVLRLSSPVEPSQRLQEIWRQLAGLGGGRPLGFGPNRVGSLPD
                     GVAQVLAEYMEQRAERIALEEPMRLDDLLKDTQDPNQKASEKSKKDQCALKIGDLCPE
                     CGQAALVNEEGCRKCYECGYSEC"
     gene            3759..4406
                     /locus_tag="PLL88_11110"
     CDS             3759..4406
                     /locus_tag="PLL88_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOJ02153.1"
                     /translation="MAKKLILVAGNIGSGKTSLTERIGEQLGWRTAYESVVNNPYLPN
                     FYKDMRSWSFHLQVYFLGHRALQHQEMAIDPRSAIIDRSIYEDAFIFCRALNRMGNLT
                     ELDYQTYLQLFNLVVAQLPAPDLLIYLHCPVTALMERIHQRGRDMESSITADYLTLLE
                     SFYTDWMHTFDICPVLTIRTDDLDFVHKKKHLDLVIARIQDKLAGKEEMVFDKEH"
     gene            4512..4757
                     /locus_tag="PLL88_11115"
     CDS             4512..4757
                     /locus_tag="PLL88_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02154.1"
                     /translation="MSKAGRKLVLHEYLSRVALFLILFCVSGNWGWLGACILIPIAMR
                     ANPFFSSTVRIQSERGQTVVGQEPLIIMTTRIAGGMT"
BASE COUNT         1250 a         1270 c         1168 g         1138 t
ORIGIN      
        1 atcaaagaga cagcgcatcc tgacgtcaat ctcatcttcg gcgcagtgat cgatccaaat
       61 atgggcgacg aatgccgtat cacggtcatc gccaccggtt ttgaccaggc tggcatgcgt
      121 tcccgcttgc gccatttcag tgatctcaat gagaagaaaa ccgacgaaaa ccgttttagt
      181 gcccgcccgg tgaaggataa tcctgaatcg atccgaacgc agtatgattt tcaaccgcgt
      241 gtttacaaca ctgaggacat tgaaaaaatt cctgctttta tcaggaatcg tgaaaacgat
      301 taactgagat cttaggaaat cctttttctc ctttgtaagg ggtatgagct ctcacccggt
      361 tcatacccca accttcaaga acggatatat ccttttctcc aatctatcgc tacgctgtcc
      421 cacttttatc attaaaaaat tgtaatattt aagattgttt ttcccttctt acttgaaacc
      481 agaatttatt tatgctagaa tgaccccact aatgtataca tgccgctatt gatcccccac
      541 atatggggga tttttcagag ttttgcgtca cgcaacgaag tgaaacattt tccaaatcat
      601 caaggagtaa gctatgcgct gtccgcattg tgaaagtgag aataccagag tgatcgatac
      661 cacccacgat gccaaagggg gaatccgccg ccggagaata tgcgaagatt gcgaccagcg
      721 tttcagtact tacgagcgcc cagtcctttc caccccgctg atcatcaagc gcgataatac
      781 ccgcgaagaa ttcgataagg agaaattact tcagggaatc cgcctggcat gtgccaagcg
      841 acccgtgcca gcagaaaaga tcgaacgcct ggctggggaa atcgaagcca agttacaaca
      901 ctctaccaag ttagaaatcc cttcaaaagt cataggcgat ctcgttattg aaggactgcg
      961 aaaattggat gatgttgctt acattcgctt tgcgagcgtt tatttgccgt tgggcgatct
     1021 gaacgcacta cgcgacgaga tcgaccgttt attagaacac aaaggagaaa atcgatgacc
     1081 tcaaattcag caagccagcc gccaaaaaat gatcgcccat tcgttgaaat gccgcatggt
     1141 ctgccgaaag tcgagttcag tgaaaacagt tatcaggttt ttacaaaaag atatgtgcgc
     1201 aagggggaag atgggcaact aaccgaaaca cctgaagaga ccttctggag ggttacctgg
     1261 cacattgctg cagttgaaga caaaagcgaa gaagaaatca tcgaaacagc aagccagttc
     1321 tataacctga tggcatcgat gaaattctta cccaactccc ccacttttac cggggcaggc
     1381 actccgcttg ggcaactggc tgcctgtttt gtattaccca tcagtgacga catgggcaaa
     1441 aaaccaagcg gaatctttca gactctgcgt gatgcagcac tgatccaaca aactggggga
     1501 gggaacggtt tttcattctc ctcgctgcgc ccatccggtg cactggtaaa gtcttccgct
     1561 ggaaaagcca ccggtccaat cggattcatg cgcgtgttcg atcacgcctt cggcgaagta
     1621 gcccagggtg gcacgaggcg cggcgccaac atggctgttt tgcgggtaga tcacccagat
     1681 atcgagtctt tcatcacctg caaaacgcgt gagaacgcca tcaccaattt caacatttcg
     1741 gtcggcatca ctgatgcctt catgaaagca gtggaagagg atgccatgtg ggatctgcgc
     1801 tttccagatg tcaaccaccc cctgtatcgt gactttgagg gaacctttga agatgcagaa
     1861 agagctggca tcccactgat tatccataaa actgtgcgtg cccgtgatct ctttaacctg
     1921 atcgcattcc aggcacacca caatggcgaa cctggcatgc tctttatgga tagcgccaac
     1981 cgctccaatc cgatccccaa tttgtatagc ctggaagcca ccaacccctg cggggaacaa
     2041 tggctcggac cgtatgaaaa ttgctgtctt ggatccatca acctttccaa gcactatttg
     2101 gcagataccg gcgtggactg ggaagcccta cgttccagca tccaattggc agtgcgtttc
     2161 ttggatgacg tggtggatgc caataaatat gtaccctcgg ttccgcaatt gcgcgagaat
     2221 gcattgcgcg cccgccgcat cggtctggga ttcatggggt tggctgatct gatgtacttg
     2281 tgcggtatcc gttacggctc tgaagaaggg caagaatttg ctgcccagat catggaattc
     2341 atccgctacc acgccatgct taccagcata gaactggcaa aagaattctc acctttccct
     2401 gctatacacg atagtatcta caatcccgaa aagatgacct ggcaaccccc tcaatcgatc
     2461 gtcgcttaca cacacaattg gggtcgcccc gatttggact ggaacaaggt catcaaagat
     2521 atccgaacct atggtatccg caacgcggca ctcaccacca tcgcccccac cggaaccatt
     2581 tctacggtgg ctggctgcga aggttatggt tgtgaaccgg tttttgccct ggcttatgtg
     2641 cgccatgtgg atgatggcgg gaaagatctg gaattgacct acctgagccc gatcatgcaa
     2701 gccgctttgg atcaatggga tctcaccccc gaacagcgag aaacgatcgt tcaggaaata
     2761 ttagaagagg gctcttgcca ggcgatcgat caactgcctg aacagatccg caatgttttc
     2821 gtggtgtcgc aggatatcag cggagaggaa catgtgcgca tgcaagccgt tttgcaggcg
     2881 ttcgtagata atagtatttc taaaacgata aacttcccat ccaatgcccg cgagaacgat
     2941 attgctgctg ccttcttact ggcatggaaa ttgggatgca aagggattac tgcatacgtc
     3001 accggttctc gggaacaggt agtgcttgaa actgccgcaa cgcgcaacca aaaacaaagc
     3061 gttgaaatgc gggaaatggt ccagccgagc aacgatccgc tcagcccggt gtggttgacc
     3121 gaaaacgact atgaccaacc caacctgtgg agtgaagcca aaaagcctcg cccgcgatat
     3181 ttatcgggga tgacctacgg catcaagacc ccagtaggca agacctttat caccattaac
     3241 gaaagtggtt tcaaccaacc ctttgaagtg ttcatcaaca ccgccaaagc aggttcagat
     3301 gtggctgccg attcagaagc cctggcgcgt ctgatctctt acgtcctgcg cctctcctca
     3361 ccggttgaac ccagccagcg cctgcaagag atctggcggc aactggctgg cttgggcggt
     3421 gggcgcccgc tgggctttgg ccccaatcgc gtgggttctc tgcccgatgg ggttgcccag
     3481 gttctggcgg aatatatgga gcagcgcgcg gaacgcatcg cgctggagga acccatgcgc
     3541 ctggatgacc tgctgaagga cacccaagac cccaaccaga aagcatctga aaagtccaag
     3601 aaagatcagt gcgcgctcaa gatcggcgac ctgtgccctg aatgcggaca agcagcattg
     3661 gtaaatgaag agggctgccg taagtgctac gagtgcgggt atagcgagtg ctgaaatagt
     3721 gcaggtcttc aattggagct tcgaccactg gagaaaatat ggcaaaaaaa ctcatcctgg
     3781 tagctggcaa catcggttct ggaaagacct cgctcactga gcgcattgga gaacaactgg
     3841 gctggcgcac cgcgtatgaa tcggtggtca ataatcccta cctgcccaat ttctacaaag
     3901 atatgcgcag ttggtccttc cacctgcagg tatatttttt gggtcatcgc gctttgcaac
     3961 atcaggaaat ggcaatcgat ccgcgctctg ccatcatcga ccgcagtatc tatgaggatg
     4021 cgttcatctt ttgccgtgct ctcaaccgca tgggcaacct gaccgaactc gattatcaaa
     4081 cctacctgca gcttttcaac ctcgtagtgg ctcaactccc cgcccctgac ctgctcattt
     4141 atctgcactg cccggtgact gctctcatgg aacgcattca ccaacggggg cgcgatatgg
     4201 aaagcagtat cactgccgat tacttgaccc ttctcgaatc cttctatacc gactggatgc
     4261 acacctttga catttgcccg gtccttacga tccgcacaga cgatttggat ttcgtgcata
     4321 aaaagaagca ccttgatttg gtgatcgctc gcattcagga taagctagcc gggaaagaag
     4381 aaatggtgtt tgacaaagaa cactgacaat tcttgtaaat ttatatcatc gtcgctcttt
     4441 ttgaagtgaa tgcatagact ggaaagaagt gcttcataga taaaaatatc atctctcgaa
     4501 cctggcttaa attgagcaaa gccggacgaa agctcgtctt gcacgagtat ctttctcgcg
     4561 ttgcattgtt tctgatcctc ttctgtgtct caggaaactg gggctggttg ggtgcctgca
     4621 tcctgatccc catcgccatg cgcgccaatc cattcttctc atccaccgtg cgcatccaat
     4681 cggagcgcgg tcagaccgtt gtgggacagg aacctttaat aatcatgacc acccgcatag
     4741 cgggtggcat gacctagccc tataagggca cttcacttgc agcacctaaa agtgctgctt
     4801 tctcttcgtt caagcctggt gcaacg
//
LOCUS       DAOSDS010000143         4801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_6269_length_4801_cov_3.920565, whole genome shotgun sequence.
ACCESSION   DAOSDS010000143 DAOSDS010000000
VERSION     DAOSDS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4801
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6269_length_4801_cov_3.920565"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLL88_11120"
     CDS             <1..202
                     /locus_tag="PLL88_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02155.1"
                     /translation="SLTNVQLLLDFQDGIYPTCWQISTPPPVFPQVEQWWVGNPLYLT
                     VKRINPQVRMMLSAISTVSNFC"
     gene            274..1089
                     /locus_tag="PLL88_11125"
     CDS             274..1089
                     /locus_tag="PLL88_11125"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOJ02156.1"
                     /translation="MKEPSPDQPAPVLYIPHGGGPLPLLGDKYHQPMVDFLRSITPRI
                     GKPSAILVISAHWEEPVATITAGANPSLFYDYYGFPDEAYQITYRAPGQPELAQKIFT
                     LLEAHRIPAKLDEERGFDHGLFIPLKIMYPDAAIPCVQLSLLNTLDPAAHVHMGKALA
                     ALRKENILILGSGFSFHNLAAFFYQRNPDGSDPQNEAFQQWLIETCTTQQLPQEEREG
                     RLIAWQNAPYARYCHPREEHLLPLHICAAMAGSPAELVFEGEIIEKKAVSFLW"
     gene            1716..2777
                     /locus_tag="PLL88_11130"
     CDS             1716..2777
                     /locus_tag="PLL88_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02157.1"
                     /translation="MLVGIIQLCLWWLVGSIIHTNFPNPEIVHLTDSDEFSFGFFLWG
                     VFIPILWWYYLSWPSILKKVIKTLGDNDVISKDKLERAINSRQKNTFTIPACLITIFI
                     LILYFSDSITVEISSGRISFWFISWWSKLILGIFVGVSAYILIVFLFKVLQTILIFRK
                     FFMKNGVKKVYVLHVDECGGFLVIGNLATRLSTLAVIVGMWAVWYSILPWLVGGKMNF
                     SSTVLMLYGAYAILAPLLLLSLILPAHYAMQRYKKARMHTYTQKIQNKFELVNTNFEC
                     EGLEITSPGKTRHYINECQKQYDFYMLLANTPEWPIKMLNLKRFTRFALLPGVGGVIS
                     FIITAYDILDLYTKIINNL"
     gene            complement(2950..3615)
                     /locus_tag="PLL88_11135"
     CDS             complement(2950..3615)
                     /locus_tag="PLL88_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ02158.1"
                     /translation="MHESELIACRKCPRLVAWREQVAREKKAAFRDWVYWGKPVPGFG
                     DKHASVMVVGLAPGAHGSNRTGRMFTGDASGDFLYPALYRAGFCNQPASTHREDGLQL
                     NALYITAVARCAPPQNKPSREEIMNCLPYLIREIELLPDLQGFVALGSIAFNTLLYIY
                     KMHFGIPFIEKPLFRHAALYRFSDAPWLLASYHPSRQNTQTGKLTPIMFDKVWEKVRE
                     LLG"
     gene            complement(3632..4651)
                     /gene="queA"
                     /locus_tag="PLL88_11140"
     CDS             complement(3632..4651)
                     /gene="queA"
                     /locus_tag="PLL88_11140"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOJ02159.1"
                     /translation="MKTQDFDYELPPEYIAQTPLEPRDSSKLLVLSRKDGSITHSVFH
                     DIGNYLHPGDLLVVNKTRVIPARIYAHKPTGGKVEILLLRKNADGSWLALLGGKRLKA
                     GMHLSIERGPQVEIMAEEDGANRVVRFSQPVEKFLPAIGNTPLPPYIHKKLRDPERYQ
                     TIYGKVAGSAAAPTAGLHFTETLIRSLKSGGVGYAEVLLHVGLDTFAPVSEENVEEHP
                     IHREWCQVSEETVRQVRATKRGGGRVIAVGTTTVRTLESAARDVPNDEVIRSYEEMTD
                     LFILPGYPFRVVDALITNFHLPKSTLIMLVSAFAGWENIRSAYEVAKEKRYRFYSFGD
                     AMFIR"
     gene            complement(4648..4791)
                     /locus_tag="PLL88_11145"
     CDS             complement(4648..4791)
                     /locus_tag="PLL88_11145"
                     /inference="COORDINATES: protein motif:HMM:NF017317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HOJ02160.1"
                     /translation="MDVVEPYLLQIGFLSRTPQGRCATRLAYEHLGYSFSDKPSQPSL
                     IQS"
BASE COUNT         1237 a         1249 c         1056 g         1259 t
ORIGIN      
        1 ttcactcaca aatgtgcaat tgcttcttga ctttcaagac ggtatctacc cgacttgttg
       61 gcagatcagc actccacctc ctgtttttcc acaagtagag cagtggtggg ttggaaaccc
      121 actctacctg actgtgaagc ggataaatcc gcaagtcagg atgatgctct ctgctataag
      181 caccgtatcc aatttctgtt aaagctttct ataaatttcg cctattccat actatttgat
      241 gtatgatgaa cctggatacc tatggaggca catatgaaag aaccatcccc cgatcagcct
      301 gctcctgttc tctatatccc acatggcggt ggacctctcc ctctgcttgg cgataaatat
      361 catcaaccca tggtggattt tctccgttcc atcacacccc gcatcggcaa gccctctgcc
      421 atcctggtta tcagcgccca ttgggaagaa cctgtcgcca ccatcactgc cggggcaaat
      481 ccatcccttt tctacgatta ctacgggttt cctgacgaag cctatcagat cacttaccgc
      541 gccccgggac aacccgaact ggcgcaaaag atattcacgc ttctcgaagc gcatcgcata
      601 cctgccaaat tggacgaaga gcgtggcttt gatcacggtc tcttcatccc attgaagatc
      661 atgtacccgg atgcagcgat tccgtgcgtt caattatccc ttttgaacac tcttgacccg
      721 gcagctcatg ttcatatggg aaaagccctg gcagcattac gaaaagaaaa tatcctgatt
      781 cttggctccg gattttcttt tcacaacctc gccgcttttt tctaccagcg taatccagac
      841 ggctccgatc cgcagaacga agcatttcaa caatggttga tcgaaacctg cacaacgcaa
      901 caattgccgc aggaagaacg agaaggaagg ctgatcgcgt ggcaaaatgc gccctatgcc
      961 agatattgcc accctcgcga agaacatttg ctgccgctgc atatatgcgc cgcaatggct
     1021 ggttcacccg ctgaactggt gtttgaagga gaaatcatcg agaaaaaagc tgtttcgttt
     1081 ttgtggtaag tgtgcaaaag agcaaacccg cgttgattaa aaaaagcgat cccgcatcca
     1141 agcggaggac gcttttgcat gaatatggta aaactccttg ttcccgattt ttcttcctga
     1201 agcctccgat caaggcaggg caccccacca gcagcaaccc taatatgacc acgaacgtgt
     1261 tctgtaagat tgtcacgaaa ctgaattttg gcaaagttcg ggtggttagt acgccattac
     1321 gatagaaatt ccacaggata ggcagcgcca gcccataaag ggtgatgaga gcgaacttca
     1381 aactaatcag ggtcgcagag actagtacca gcttgtgctc ctctattcca cattcaaatt
     1441 tcccttttca gtttttcatt gaataaaaaa gataatcaac ctttttaata attaatcaaa
     1501 aacaagtatc cgaatcaata atctttcagt tggattgtgt ttctaaaaat gcctatataa
     1561 tcaatacaca ttattatcct gggtcatccc ttcaagaggg tcacatgtcc aacaaaaaaa
     1621 atatttccaa actatacaaa aagttgatcc aggtacagga tcccatttac ccgcttgcac
     1681 agaagatttc ccgaggccgc ccgatcatcg gggctgtgct ggtcgggatc atccaactat
     1741 gcctttggtg gttagtaggt tctatcatcc atacaaactt ccccaatcct gaaattgtcc
     1801 acctcaccga ctcggacgaa ttctcttttg gcttttttct ttggggcgtc tttattccaa
     1861 ttttatggtg gtattacctc tcctggccct ccattctgaa aaaagttatc aaaactctgg
     1921 gcgataacga tgtaatctca aaagataaat tggaacgggc tatcaacagc cgtcaaaaaa
     1981 atactttcac catccccgct tgtttgatca ctattttcat tcttatactg tatttcagcg
     2041 atagtattac tgtggaaatc tcttcgggaa ggatcagttt ttggttcatc agctggtgga
     2101 gtaagctaat actcggtatt tttgttggcg tatccgcgta cattttgatc gtatttttgt
     2161 ttaaagtcct gcaaaccatt ctaattttcc gtaagttctt tatgaaaaat ggcgttaaga
     2221 aggtatatgt tctgcatgtg gacgagtgtg gtggtttttt agtgatcggg aatctggcta
     2281 cccgtctttc cactctggct gttatcgttg gtatgtgggc ggtgtggtat tcaatcctac
     2341 cctggctggt aggtggaaaa atgaatttta gttcaacagt gttaatgcta tacggtgcat
     2401 acgctatcct tgcacctctt cttttacttt ccctgattct tcctgcacat tatgccatgc
     2461 aacggtacaa aaaagcccga atgcacacct atacgcaaaa aatacaaaac aagttcgaac
     2521 tggtcaatac caactttgaa tgcgagggat tggagatcac atctccagga aaaacacgac
     2581 actatattaa cgaatgtcaa aaacaatatg atttctatat gctgcttgca aacactcctg
     2641 agtggccaat taaaatgctt aatctaaagc gttttacccg ctttgccctg ttacctggcg
     2701 taggaggtgt tatctccttt attattactg cctatgatat tctcgatcta tatacaaaaa
     2761 taataaataa tctttaaaaa tgaacttttt tgaaaatata gtccgaaaaa tctcattgca
     2821 acgcacccaa cttattgcaa aattactcaa tagattttaa aaaacatttg tgctagatca
     2881 ccttagcaga ttttgcgctt ttgtcttttc atccctgtag gattctgcta ctatccaccc
     2941 cgctccattt catccaagca gttcccgtac cttttcccac actttatcga acatgattgg
     3001 ggtcagcttg ccggtctggg tgttctggcg gctgggatgg taggatgcca gcaaccaggg
     3061 agcatcagag aaacgataca acgccgcatg gcgaaaaagt ggcttttcga taaagggtat
     3121 accaaagtgc attttataga tatacagcag ggtattgaag gcgatgctgc ccagagccac
     3181 aaaaccctgc aggtctggca gcaattcgat ctcacggatg agatatggca agcagttcat
     3241 gatctcttcg cggctgggtt tgttctgcgg tggagcgcag cgcgcaaccg cggtaatata
     3301 cagggcattc aactgcaaac catcctcacg atgggtggaa gcaggttgat tgcagaagcc
     3361 agcccggtac agcgccgggt aaaggaaatc cccggacgca tcaccggtaa acatacgccc
     3421 ggtgcggttc gaaccatgcg cccccggtgc caagcccacc accatcacgc tggcatgctt
     3481 atctccaaac ccgggcacgg gttttcccca gtacacccaa tcgcgaaatg ccgccttctt
     3541 ttctctggct acctgctcgc gccaggcgac caagcgcggg cacttgcggc aggcaatcaa
     3601 ttcgctttcg tgcatttccc aatccatagg ttcaccggat gaacatggca tcaccaaaag
     3661 aataaaagcg atagcgcttt tctttcgcca cctcataagc gctgcgaata ttctcccagc
     3721 ccgcaaaagc gctcaccagc atgatgagcg tggactttgg caaatgaaaa ttggtgatca
     3781 gcgcatcgac tacgcgaaaa ggataaccag gcaaaatgaa cagatcggtc atttcctcat
     3841 aagaccggat gacttcatcg ttcggcacat cccgtgccgc cgattccagg gtgcgcacgg
     3901 tggtggtccc caccgcgatc acccgccctc ccccgcgttt tgttgcacga acttgccgaa
     3961 ccgtttcttc gctcacctgg caccattcgc ggtgaatagg atgctcttcc acattttcct
     4021 cacttaccgg cgcgaaggta tccaggccca catgcagcag cacttccgca tatccaacac
     4081 cgccgctctt cagggagcgg atcagggttt cggtgaagtg cagacctgcc gttggagccg
     4141 ctgccgaacc ggcaaccttg ccgtaaatgg tctggtagcg ttcagggtcg cgcaactttt
     4201 tatggatgta aggcggcagg ggagtattcc caatggcggg taaaaacttt tccactggct
     4261 ggctgaaacg aacgacacgg ttggcgccat cttcctctgc cataatctcc acctgcggtc
     4321 cgcgctcaat gctcagatgc atgccggctt tcaagcgttt ccctcccagc agcgccaacc
     4381 agcttccatc cgcattcttg cgcagcaaca gaatctccac ctttccgccg gtgggtttgt
     4441 gcgcgtagat gcgcgccggg atgacacgcg tcttattcac caccagcaaa tcgccgggat
     4501 gcagataatt cccgatgtcg tgaaaaacgg aatgggttat gctgccgtct ttacgcgaaa
     4561 gtaccagcag tttcgaagaa tcgcgcggtt ccaatggagt ttgcgcaata tactccggtg
     4621 ggagctcata atcgaaatcc tgtgttttca cgattggatc aaactcggtt gagagggttt
     4681 atcgctgaag gaatagccca gatgctcata agccaagcgg gtggcgcacc tgccctgcgg
     4741 ggtacggctg aggaagccaa tctgcaacag gtagggctct accacatcca tgatggtatc
     4801 c
//
LOCUS       DAOSDS010000144         4793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_6281_length_4793_cov_4.163993, whole genome shotgun sequence.
ACCESSION   DAOSDS010000144 DAOSDS010000000
VERSION     DAOSDS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6281_length_4793_cov_4.163993"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..894)
                     /locus_tag="PLL88_11150"
     CDS             complement(139..894)
                     /locus_tag="PLL88_11150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ02161.1"
                     /translation="MDLELHGKVAIVTGGVRGIGAGMCEVFAEEGANVVIADMVPLEE
                     SRKIAAQLSAKHNVRTLAVRGDISDPKDVAEIYQQTLEKFGTVDILMNNAGKMGVTKI
                     EDVTVEELYKYEKVNIEGMFLMSKEFIKICNEQKKGGHIVNTISKSAFTTNSGGNTPY
                     IATKGAVFSFTRGLAKEVGSRGIFVNGIVPGYVITDTTRNLKERSKAMKKIIPLGREA
                     TPREIANVAVFLCSPKAVQMMGAIVDVSGGTML"
     gene            complement(909..2003)
                     /locus_tag="PLL88_11155"
     CDS             complement(909..2003)
                     /locus_tag="PLL88_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOJ02162.1"
                     /translation="MKLVCIIEKKIPALREPILRFARRFEGVEVIESLWDDPSEADIT
                     AVIRRIEKNGPANEPVPQGFIEHKDAELLAGSFCPFSAQGMDVFDNLRIIGVMRAGLE
                     NIDVRAASERGIAVVNASGRNADAVSDFAIAMLLAEARNIARAHHSIVSGGWQVDFAS
                     SATTPDMRGKTVGLFGFGAIGKALTEKLGGFHINLMVYDPYIEQESVAAFGGKLVDKE
                     TLFKESDFISVHARLTDENQRTIGKAEFSLMKKSAYFINTARAGLVDYDALYAALAEK
                     RIAGAALDVFDNEPLPVDDRFRKLDNVTLTTHRAGATLDAANNSPRLVFERMRNLIAG
                     SAMEGLVNPQVLDDPKFKAWQKKAKETLDL"
     gene            complement(2113..2421)
                     /locus_tag="PLL88_11160"
     CDS             complement(2113..2421)
                     /locus_tag="PLL88_11160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOJ02163.1"
                     /translation="MTLKILAACGQGLGSCQIITMKLNAVFKKMGIDARVTSSNVSMA
                     KSTATSYDVLFCGQTLVPNFKTAESKGVVVIGLKNLVSEKEMEEKIHEAIASGKIKTN
                     "
     gene            complement(2456..3961)
                     /locus_tag="PLL88_11165"
     CDS             complement(2456..3961)
                     /locus_tag="PLL88_11165"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HOJ02164.1"
                     /translation="MFLTAWNWFVANIFTKPAWFLALMVFIGCLLIKKPIYEAFASFV
                     KALVGYMIFNVASNGMVATFRPILFALNGRFNLQAAVIDPYFGAAPLQKSITEVAGRT
                     MSIATMAMILGFAINVLLGYFKKQTKVRSLMVGGHVMNLGSWGVIAYLVLMIPSISDL
                     GAIISGGLLCGLLWGVLSNLTVEPTQELTDGANIAVGHVQMTGIYIFDKLATWIGNMD
                     KKKGKEIHKIGDIKLPGFLSIMNDTTVAASIVMLLFFGGMMLIIGREYMLTVDESLAS
                     QSWGFYLIEKCLTFVVYLNIIILGIKMFVGELTEAFSGISQKILRGAVPAVDIAATFG
                     FTEGSVVTFGFLFGALGSIIAILLSVLFKNPILAIVGFIPMFFDNGGIATFTNNKGGL
                     KAVIISTLIVGMVHVFGGGLYAYFYGFSNYGGAAANFDIAAWHTLLGVLMKNLGFVGI
                     GVICLALILIPQLQYLKNKDTYFLVVDDYEKYKEVIAKKTSARPAAKKAEK"
     gene            4300..>4793
                     /locus_tag="PLL88_11170"
     CDS             4300..>4793
                     /locus_tag="PLL88_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02165.1"
                     /translation="MGKIRTVLGDIDSSTLGFTMAHEHVFMDIAGHGKPDIEWSLYRW
                     NEQLQILREYKAVGGNAMIDANPRYLDGRDAARMKVASEQSGVHIVACTGFVKEAENQ
                     ANCQDLAKMSIEQMTEFFVKEITIGMDGTDIRAGWIKGASMYCYITPLQEKALRAAAR
                     AAMLT"
BASE COUNT         1223 a         1265 c          995 g         1310 t
ORIGIN      
        1 ctctacaatc tgtaatctca aagatgtttt tcgatggatc ataaccgccc atgagtctat
       61 tatataatgg atgtcaattt ttaaacgcca tgatttgggt ttgccaaaga gataatttcc
      121 tttccttttg ttcaaacact acaacatcgt cccgccactg acatccacga tcgcccccat
      181 catttgcacg gctttggggg aacacagaaa gactgccaca ttggcgatct cgcggggggt
      241 ggcttctctc cccagcggaa tgatcttctt catcgctttg gatctttctt tgagatttct
      301 ggtcgtgtcc gtgatcacat agccgggcac gatcccattc acaaagatgc cgcgcgagcc
      361 cacttccttc gccagccctc gggtaaacga aaagaccgca cccttcgttg cgatataggg
      421 cgtattgccg ccgctgttgg ttgtgaaagc cgatttggaa atcgtgttga cgatatgccc
      481 gcctttcttc tgttcattgc agatcttgat gaactccttg ctcatcagaa acattccttc
      541 gatgttcact ttttcatatt tatacagttc ttcaacggtc acgtcttcga ttttcgtcac
      601 acccattttt cccgcgttgt tcatcaggat gtcaaccgtg ccgaattttt ccagcgtttg
      661 ctggtagatc tctgccacgt cctttgggtc agagatatca cctcgcacag ccagcgtgcg
      721 cacattgtgt tttgcactca actgcgctgc gattttgcgc gattcttcca gcggcaccat
      781 atcggcaatc acaacattcg ccccttcttc cgcgaacact tcgcacatgc cagccccgat
      841 tccccgcaca ccaccggtca cgatcgccac ttttccatgc aattccaggt ccataaaaaa
      901 ttctcctctt acaaatcaag cgtttctttt gcttttttct gccaggcttt gaactttggg
      961 tcatccaaaa cttggggatt gaccagccct tccatcgcgc ttccagcaat caagtttcgc
     1021 attctctcaa agaccagacg cggcgagttg ttcgccgcat ccagggttgc ccccgcccga
     1081 tgcgtggtca gcgtgacgtt atccagcttg cggaatctgt cgtcgaccgg cagcggttcg
     1141 ttatcaaaaa cgtccagcgc tgcaccggcg atccgttttt ctgccagcgc agcatacagc
     1201 gcatcataat ccaccaatcc ggctctcgcc gtgttgatga aataggcgct cttcttcatc
     1261 aggctgaatt ccgcttttcc gatcgtgcgt tggttttcat cggtcaatcg cgcatgcaca
     1321 ctgatgaaat cagattcttt gaacagggtc tccttatcaa ccagctttcc gccaaacgcc
     1381 gccacgcttt cttgttcgat gtaggggtcg tacaccatca ggtttatatg aaaaccgcct
     1441 agtttctcag tcagtgcctt gccaatcgct ccgaacccaa acaaaccaac ggtcttgccg
     1501 cgcatgtctg gcgtggtagc gctgctggca aaatccacct gccatccacc gcttacaatg
     1561 gaatggtgtg cacgggcgat gttccgcgct tctgccaaca gcatagcgat ggcgaaatcc
     1621 gagactgcat ctgcatttcg cccgctggca ttcaccaccg caataccccg ctcgctggcg
     1681 gcacgcacat caatgttctc caaaccggct cgcatcactc cgataatacg caggttatcg
     1741 aacacatcca tgccttgtgc tgaaaaggga caaaaacttc ccgccagcaa ttcggcatct
     1801 ttatgctcta tgaatccctg gggaactggc tcgtttgccg gtccgttttt ctcaattctg
     1861 cgaatgaccg ccgtgatatc cgcttcactc ggatcatccc ataatgattc aatgacttca
     1921 acgccctcga atcgccgtgc aaagcgcaag atcggttctc tcagggcagg aattttcttt
     1981 tctatgatac ataccaactt catgcaaaaa cctctttttg tgttctcagg tcattttctg
     2041 gggtctactt cttgtatcgc atctttaaat tagtggaggg cactatcaaa atgccctcca
     2101 ctttctcgac aattaattgg tcttgatttt tccactggca atcgcttcgt ggatcttttc
     2161 ttccatttct ttttcagaaa ccaggttttt caagccaatg acaacgacgc ctttcgattc
     2221 agcggtttta aaattcggca ccagtgtctg cccacaaaat aacacgtcat aactggttgc
     2281 ggttgatttt gccatggata cattacttga ggttaccctg gcatcaatgc ccattttttt
     2341 gaaaacagcg ttcaatttca ttgtgataat ctggcagctt cccaatcctt gcccgcaagc
     2401 agctaaaatc ttaagcgtca tttctctaac tccttttttc gcagaaaatc atatcttatt
     2461 tttctgcctt ctttgcggct ggtctggcac tggttttctt tgcgatcact tctttgtatt
     2521 tttcgtagtc atctaccacc aagaaatacg tatctttgtt ctttaaatat tggagctgcg
     2581 gaatgaggat caaagccaga cagatcacgc caatgccaac gaatcccagg ttcttcatga
     2641 gaacgcccaa cagcgtgtgc cacgccgcga tatcaaagtt ggctgctgca cctccatagt
     2701 tggagaaccc atagaaatag gcatacaatc cacctccaaa tacatgcacc attccaacga
     2761 tcagagtaga aatgatcact gcttttaaac cgcctttatt gtttgtgaat gttgcaatac
     2821 cgccattatc aaagaacatg ggaatgaatc caacgatcgc cagaattggg ttcttgaaaa
     2881 gtaccgataa tagaattgcg atgatggacc ccagagcacc aaataagaag ccgaaggtta
     2941 caacactgcc ttcggtgaac ccaaatgttg ctgcaatatc cactgccgga acagcaccac
     3001 gcaagatttt ttgggaaata ccgctaaaag cttccgttaa ttcacccacg aacatcttga
     3061 tacccaggat gatgatgttt agataaacta cgaaggtgag gcatttttcg atcaggtaaa
     3121 agccccagct ctggcttgcc agtgattcat ccacggtcag catatactct ctgccaatga
     3181 ttaacatcat tccaccgaag aacagcagca ttacgatcga ggctgctacg gtagtgtcgt
     3241 tcatgatcga caggaaacca ggaagtttga tatcaccgat tttatggatc tctttccctt
     3301 tcttcttatc catattgccg atccaggtag ccaatttatc gaagatgtaa atgcccgtca
     3361 tctggacatg tcctacggca atgttcgccc catccgttaa ttcctgggtc ggttccacgg
     3421 taagattgga gaggacgccc cacagcaaac cgcacagcag accgccagaa atgatcgcac
     3481 ccagatcact gatactagga atcattaaaa ctagataagc gatgactccc caggagccca
     3541 ggttcataac atgcccaccc accatcagcg agcggacctt ggtctgcttt ttgaaataac
     3601 ccaggagaac gttgatggcg aacccaagga tcattgccat ggttgcaata ctcatggttc
     3661 tgcccgcaac ttctgtgatc gatttctgca agggggctgc tccaaaataa gggtcgatca
     3721 cagcagcttg caggttaaat cgtccattca gagcgaacaa gatcggtcgg aaggtcgcaa
     3781 ccatgccatt ggaagccacg ttgaagatca tgtaccccac caatgctttc acaaaactcg
     3841 caaaagcttc ataaatgggt ttcttgatca gcaggcatcc gatgaatacc attaaagcca
     3901 gaaaccaggc tggtttcgta aatatattgg caacgaacca attccacgcg gtaagaaaca
     3961 tgtttttacc acctttctaa atctaaaata tttaaaattg attccctctt cgtactacaa
     4021 gcactttttc caactctacg tcatacaaaa atcttatcga tatgtgtact tcttttcacc
     4081 tcctgatatg aatttttata actttctgaa taatttctct aggaagatac tagtattggc
     4141 aatttttttt gtcaacgaaa ccgcaatgaa atcacatttg tataactcgc acaattgtgc
     4201 aggaatggta tttgtgcttt gggcgtaaaa tcgttgactt tttagaatcc cacatttacg
     4261 ataaaaaaaa tcacacatca tcaaaaaaga ggtcacagta tgggaaagat tagaacggtc
     4321 ttgggagata ttgattcctc tacgcttggc tttaccatgg cacatgaaca tgtttttatg
     4381 gatatagccg ggcatggtaa acctgatatc gaatggagtt tatatcgctg gaacgagcag
     4441 ctacagatct tacgcgagta taaagctgtg ggcggaaacg ccatgatcga tgccaaccct
     4501 cgctacctgg atggacgcga tgccgcccgc atgaaagtgg cttcagaaca aagtggcgtg
     4561 catatcgtgg cttgtaccgg gtttgtgaaa gaagctgaga atcaagccaa ctgccaggat
     4621 ttggcgaaga tgtcgatcga gcaaatgacc gagtttttcg tgaaagagat caccataggc
     4681 atggacggca ccgacatcag agccggatgg atcaaaggtg ccagcatgta ttgttacatc
     4741 acaccccttc aagaaaaagc gctgcgcgcc gccgctcgtg ccgcaatgct cac
//
LOCUS       DAOSDS010000145         4699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_6429_length_4699_cov_4.376184, whole genome shotgun sequence.
ACCESSION   DAOSDS010000145 DAOSDS010000000
VERSION     DAOSDS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6429_length_4699_cov_4.376184"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="PLL88_11175"
     CDS             <1..381
                     /locus_tag="PLL88_11175"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ02166.1"
                     /translation="RDEGTSYHYLPPSRECCPSPGAVTVMETTLKGAKVPFLPVKTWT
                     TDAFYRETAGKRERRIMEGCRVVEMEAAALFAVAEFRKVELGMLLYCGDLVVPQGWQE
                     RAWNQRSESRTLLFDLAAQACINL"
     gene            complement(394..606)
                     /locus_tag="PLL88_11180"
     CDS             complement(394..606)
                     /locus_tag="PLL88_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02167.1"
                     /translation="MRPVRRTSAGYNPAAREEHWTARGGAVIDTLFLDASIGDEMPCL
                     SGSLGFHPVVMHTSGSTATATAASTS"
     gene            complement(672..2771)
                     /locus_tag="PLL88_11185"
     CDS             complement(672..2771)
                     /locus_tag="PLL88_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02168.1"
                     /translation="MTEKNQIFDIIDGALTGKELLDQPRAFLLLRLCQFCLSFDPERA
                     EKYWLMLSGQSRALPQENQAELTELRTNFEEAVSTEKKGFAAEMLAEIAEIKKLESDE
                     EKKTKLKDCEARLKKRFNPLGKGPVWKALVEVWLPLDHPYAFQLMKNISGRLQNDILK
                     RLNRAALLEPNEWTLLSQTLGAGKIESLILEILADENQTIRLEEPLIEQVAKKIRAGL
                     PQLSMPLNTELLTKNMHNHARLLALHAAKDKENLLGRLIAEMVEDLAKASWLDPVWLE
                     RFNLIQSILNTGAQLESQGLSILTPSFADTIVNKLPPYLQKFFLATRAGLSAKADAIP
                     AAYQDLMARTSNDEISEAWFFVILVQRGFCKEAMQEAQKSPRADTLLPRLRRAWICSN
                     PTTACTVIKPSDMAGDAIGEFLAMGTSEKRAEHLKNSTDNGKHGVPGAMWAGVGTEDN
                     SEGVRGFWASLTAHQKTFDEIIQEYLTLNPLYSSYTRNTPKEQQFDVHLAVNGFGRYT
                     YQEIDSALLGALVAWAEKEQAPVYSVLQAMWNAIRPDDNILRVDWLRNAILSRCVTVF
                     GADQNVLFNDYLNWLQVELVQKGRQWQIGKQIITLRYPPTSPLQFSLISASAISAFST
                     NRRDQIIIGALQKYEGNPQLIENAAMLYNGGKTIMDLTPPTTIKQNFLPNWQTGIVKN
                     ALPSILQVLILGKVNPT"
     gene            2980..3927
                     /locus_tag="PLL88_11190"
     CDS             2980..3927
                     /locus_tag="PLL88_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02169.1"
                     /translation="MSDSIFFAWSNCIISKKGGRIISISIIDNRIATENDEGEMAGIA
                     EIYIDEIRKQNKDFYATWIPGKPMKLGDMGIIEKNQFTLVGNLKDQGVKFKVRRDPSP
                     MPYQFNSKDGVKVVFKPGGKVDPKFSDILKAEVGFGVEFGKKGKFFFKASKLYEDCID
                     DLITLGRDVQKIFQGKVEDKDYVVIDRLIKSPCVTILISKEANSKVSLIAKTDISLKT
                     VNIADASIELSVKSSSGLAFDCPNMINATPFFGVRSVRKLSKIAADFRPVTLKPKMKS
                     LTKGKVYTDSNAEMPFLGQDIRLLLEKTIVRPTALNEYK"
     gene            complement(3937..>4699)
                     /gene="lipA"
                     /locus_tag="PLL88_11195"
     CDS             complement(3937..>4699)
                     /gene="lipA"
                     /locus_tag="PLL88_11195"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOJ02170.1"
                     /translation="EEANCPNMGECWGSGTATFLVLGDICTRRCGFCDVKRGVPLPLD
                     EEEPERVVQAVKAMELKHAVITSVDRDDDILGGARIFARIIRRIHEEAPGCSVEVLIP
                     DFRADPQALRLIMAEKPEILNHNVETVLRLYHSVQPQDRHEWSQYVLTTAKQLQPKSL
                     TKSGIMVGLGETWEEIEETMRELRAWDVDILTIGQYLQPSRKHLPIARYYSMEEFQQL
                     KDYGYAIGFRWVESAPLVRSSYHAAEQVRALSTQF"
BASE COUNT         1086 a         1144 c         1259 g         1210 t
ORIGIN      
        1 cgcgatgaag gcacctctta ccattatctt cccccttcca gagaatgttg tccatcgcca
       61 ggcgcggtca cggtcatgga aacaacattg aagggtgcga aagtgccctt cttgcccgtc
      121 aaaacctgga ccaccgacgc tttttaccgc gaaacggcag ggaaacgcga acggcgtatt
      181 atggagggct gccgggtggt ggaaatggaa gctgctgcct tgtttgcggt ggcagagttc
      241 cgcaaggtgg agttggggat gttgctctac tgcggtgacc tggtggtgcc gcagggctgg
      301 caggagcgcg cctggaacca acgcagcgaa agccgcacgc tgctctttga tctggctgcg
      361 caagcttgta taaatctgta gaggaaagcc gtttcaggat gtgctggcgg cagtggcggt
      421 ggcagtgctg ccgctcgtat gcatcacgac cggatggaat cccaatgaac ccgacaggca
      481 tggcatctca tcacctatgc tcgcatccaa gaacaatgta tcaataactg cccctcctct
      541 agccgtccaa tgttcttccc tggctgctgg gttgtatccg gcggatgttc tccttacggg
      601 tcgcatgacg tgcccatcct gtttgaacca gtccttttcc tttggaaaag gactggtggt
      661 tgtgttttga cctaggttgg gttgaccttg cccagaatca atacctgcaa tatggaaggc
      721 agggcgttct tcacaatgcc ggtctgccag ttgggcagga aattctgttt gatggtggta
      781 gggggagtga ggtccatgat ggtttttcca ccgttataga gcatggcggc attctcgatc
      841 aattgcggat tgccttcgta tttctgaagg gcgccaatga tgatctggtc acggcggttg
      901 gtcgagaaag cgctgatcgc actggcagag atcaggctga actgcagggg tgaggtaggt
      961 ggatagcgca gggtgatgat ctgtttgccg atctgccact ggcgcccttt ctgcaccaat
     1021 tcaacctgca accagttcag gtaatcattg aacaacacgt tttggtcagc cccaaaaacg
     1081 gtcacgcaac gcgaaaggat ggcgttgcgc agccaatcga cccgcaggat attgtcatcc
     1141 gggcggatgg cattccacat ggcttgcagc acagaataga ccggggcttg ttccttttca
     1201 gcccaggcta ccagcgctcc tagcagggca gaatcgatct cctgataggt atatctgcca
     1261 aagccgttca ctgccagatg aacatcgaat tgttgttcct ttggggtatt tctggtgtag
     1321 gatgagtaaa gcggattcaa ggtgaggtac tcttggatga tctcatcgaa agtcttttga
     1381 tgggcagtga gagaagccca gaaaccgcgt accccttcgc tattgtcttc ggtacctaca
     1441 cccgcccaca tggctcccgg tacaccatgt tttccattat cggtactgtt ttttaagtgc
     1501 tcagcgcgtt tttctgaagt acccatagcc aggaattctc cgatcgcatc ccctgccata
     1561 tcagaaggtt tgatgaccgt gcatgcagtg gttgggttgg agcagatcca ggctcgacgc
     1621 aaccttggta agagcgtatc agcgcggggc gatttctgtg cctcttgcat ggcttcttta
     1681 cagaagcctc tttgaacaag aatgacgaaa aaccatgctt cgctgatctc atcgttcgat
     1741 gtgcgtgcca tcaggtcctg gtatgcggcg ggaatggcat cggctttcgc gctcagcccc
     1801 gctcgggtgg caaggaagaa tttttgcagg tagggtggca gtttgttgac aatggtgtcc
     1861 gcgaatgaag gggtcagaat gctcaatccc tggctctcca actgagcgcc agtgttcaag
     1921 atcgattgga tcaggttgaa ccgttccagc cataccggat caagccaact ggcttttgcc
     1981 aggtcctcga ccatttccgc aatcaaacga cccagcaggt tctctttatc ttttgcggcg
     2041 tgtaaagcca gcagccgggc gtgattgtgc atgttcttgg tcagcaattc tgtgttgagc
     2101 ggcatgctaa gttgcggcaa tccagcgcgg atcttcttgg cgacctgctc gatgagaggt
     2161 tcttccaaac gaatggtctg gttctcatca gccaggattt ccaggattaa actttcgatc
     2221 tttcccgcac caagggtttg tgagagcaaa gtccattcat taggttcgag taaagcggca
     2281 cggttcagac gttttaagat gtcattctgt aatctgccag aaatattctt catcaattgg
     2341 aaggcgtatg gatggtcgag cggcagccac acctctacca acgccttcca aaccggtccc
     2401 tttccgaggg ggttgaaacg ttttttcaag cgtgcttcac aatctttcag ttttgttttc
     2461 ttttcttcat cgctttccag ctttttgatc tctgcgatct ctgccagcat ctcggcggcg
     2521 aaccctttct tttcggtcga aaccgcttct tcaaagttgg tgcgcagttc ggtcaattct
     2581 gcctggtttt cctgaggaag agcgcgtgac tgccccgata gcatcagcca gtacttttca
     2641 gcccgctctg ggtcaaagga aaggcagaac tgacacaacc gtaatagcag gaacgccctg
     2701 ggttggtcca gcagttcttt gcctgtcaat gccccatcga tgatgtcaaa tatctgattc
     2761 ttttcggtca ttcaatttcc tccatatata gtaagaatga tttctttcgc ggtaggggaa
     2821 gaggaacatg gctggcgggc aagatgtgca catattttcc tgcaggatgc cagcaagaat
     2881 aatagatttc cgccagtaaa aacctttgaa atgccctccg tgaagatgtg tttattatat
     2941 cgaaattgga aaggaataaa aatattctat caatttctca tgtctgatag tatctttttt
     3001 gcctggtcga attgtataat atcgaagaag ggtggcagga tcatttcaat ttcgataatc
     3061 gacaaccgta tagccacaga aaatgacgag ggcgaaatgg ctggtattgc tgaaatctat
     3121 attgacgaga tcagaaagca aaataaggac ttttacgcaa cctggattcc gggaaaaccg
     3181 atgaagctgg gcgatatggg catcattgaa aagaatcaat ttaccctggt tggcaatctg
     3241 aaggatcagg gggttaaatt caaagttcgg cgtgatccgt caccgatgcc atatcaattc
     3301 aattccaagg atggagtgaa agtcgttttc aaacccggcg gtaaggtgga tccgaagttc
     3361 tccgacattc tgaaagcaga ggttggtttt ggcgttgagt tcgggaaaaa agggaaattc
     3421 ttcttcaagg cgtcgaaatt atatgaagat tgcattgatg atttgataac cttgggaagg
     3481 gatgtgcaaa agattttcca ggggaaggtt gaagacaagg attatgtggt cattgaccgt
     3541 ttgatcaaat ctccctgtgt gacgatcttg atttctaaag aggcaaactc aaaagtgtcg
     3601 ctaattgcaa aaactgacat atcgttgaaa acggtcaata tagccgatgc cagtatcgag
     3661 ttgtccgtaa aatcttcatc tggtttggcg tttgattgtc ccaacatgat caatgccacg
     3721 ccattttttg gcgttcgcag cgtccgaaag ctgtcaaaga ttgcagcgga tttccgccct
     3781 gttacgctca aaccaaaaat gaaaagcctg accaaaggga aagtctatac agattccaac
     3841 gcggaaatgc cgttccttgg tcaagatatc aggctcttgc tggagaagac gattgtccga
     3901 ccaacagcgc tcaatgagta caagtagccg gtgaaattag aactgtgtcg ataacgctct
     3961 cacctgctcc gcggcatggt agctggagcg caccagcgga gcgctctcga cccaacggaa
     4021 gccgatggca tatccgtaat ctttcaattg ctgaaattct tccatgctgt agtaacgcgc
     4081 gatgggcaaa tgtttgcggc tgggctgcag gtactgtccg atggtgagga tatccacatc
     4141 ccaggcgcgc agttcgcgca tggtctcttc gatctcctcc caggtttcgc ccaaccctac
     4201 catgatgccc gatttggtca ggcttttggg ctggagttgc ttggcggtgg tgagcacgta
     4261 ttgcgaccat tcgtggcggt cctgcggttg cacgctgtga tacaggcgca ggacggtttc
     4321 tacattgtgg ttgagaatct ccggcttttc cgccatgatc aggcgcagcg cttgcggatc
     4381 ggcgcgaaaa tcggggatga gcacctccac cgagcagccg ggggcttctt cgtgaatacg
     4441 gcggatgatg cgcgcgaaga tgcgtgcccc gcccaaaata tcatcatcgc ggtccacgct
     4501 ggtgatgacg gcatgtttca gctccatggc tttcaccgcc tgcacaaccc gttcaggttc
     4561 ttcctcatcc agcggcagag gaacgccgcg cttcacgtcg cagaaaccgc agcgccgggt
     4621 gcagatatcc cctagcacta aaaaggtggc ggtgccactg ccccagcatt cacccatatt
     4681 ggggcagttg gcttcttcg
//
LOCUS       DAOSDS010000146         4634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_6528_length_4634_cov_4.033195, whole genome shotgun sequence.
ACCESSION   DAOSDS010000146 DAOSDS010000000
VERSION     DAOSDS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6528_length_4634_cov_4.033195"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PLL88_11200"
     CDS             <1..1049
                     /locus_tag="PLL88_11200"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02171.1"
                     /translation="QVGMELEDREKSVAAAILTQLDEDGFVDVDVLELASYYHIPISN
                     VEKVKSMIQHADPIGVGSQDAREAMLVQIDELKKNKEIPAFTEKIISDHFYTLLKKQF
                     SEIAEQNAISIKQVETVADFITENLNPFPARSNWGDVRNPQENDLLQLGAPDIILSFL
                     DNNPQKQIIIEVFQPANSNLVINTSYHAALKNVDKETKVKMREDLEKASLFIKCLQQR
                     TNTILRMAQVLVEIQEKYIREGDKYLKPITRAEIARRLDVHESTISRAVSSKTIQLPN
                     RKVYPLSVFFDRSLQIRTEIKEIVKKEKEPMSDLEIVKKLEKKGMHIARRTVAKYRDM
                     EGILPAYQRKDKTK"
     gene            1046..2941
                     /locus_tag="PLL88_11205"
     CDS             1046..2941
                     /locus_tag="PLL88_11205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ02172.1"
                     /translation="MTAFIDLIKNKNLSFLEIESLLNLFNQPCVLSDEDNEIALVNSA
                     LIELTAFTKQELIRTPLRSLITEERAENGKVVYRILRKNRMPLEVELSREYLDDKHHW
                     KVYLINQKMQGDVIDYKALKSYLTTMSALIDARDETDASFYEKSLDILDKVFHFDLLC
                     FYVEENGVFQLSDLSKKTPPHFPEKILVNELENIDQYNLWQQGNRAINHLHRSAREQF
                     INVLVTQRFKKDHDQEGLIVAGWKEGVRDSSMLAIVEEYMKMVEHGASLFNQNTIHEQ
                     IRSESAKAQEILSYTLENIEEGVIVLDYEMKVLEINQNMERLLGYSKWEVQGLFVDSY
                     LVADPKITTLFHDVFERQKEQCENGVSLRQRDGDAEPVKMRVIPRDVRDPQTQFLTLV
                     RSTRELDGLKRNLKELEHQAALGKSVATFAHEVRNPINNMVTGLQVLQSMSEENEAQS
                     DLITRMMNDCVRLDHLMNSILSYAKPLENKLKPLNLDLLVKSTIEKWEAKLTRNDVKM
                     IYQCEEEAPVVMGDMRALEQVFTNLISNAVEAMKPQNGGTLAIKIEKSQAKTLTVTVS
                     DTGPGIPDEILKNLFTPFVSFSLQGTGLGLAIIKEIIAAHNGEITVDSFPGGTVFSIA
                     LPIAEGE"
     gene            2943..4121
                     /locus_tag="PLL88_11210"
     CDS             2943..4121
                     /locus_tag="PLL88_11210"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ02173.1"
                     /translation="MSFTVLIVDDEENARKNLSVFLKSKNFEVFEAGTLSEAREQLKM
                     GNGDIILLDVQLPDGYGQTLLKELQQQQNRPPVILITGFGDIEMAVDAMRNGAHDFLT
                     KPITDFKILENSLARACELIKMRREIEHYRQKQLDSVDFVLGSNEKMKNVVAQALKAS
                     RAMVSVLITGETGTGKEVLARFIHKSGPRAEKQFVAINCAAIQSTLLESELFGHDAGS
                     YTDAKTRKHGLMEIADDGILFLDEISSMPLEIQAKLLRAIEEKAFRRVGGNDLLKVDV
                     QILAASNRNLKDLIAEKAFREDLYYRLKVVDLDLPPLRERKNDIPEFVGFFIKELNPR
                     LGLNVIDVTPAAMEVLGKYSWPGNIRELRNTIERAMLFCDGEKIDVADLPLDLVEKLA
                     "
     gene            4156..4476
                     /locus_tag="PLL88_11215"
     CDS             4156..4476
                     /locus_tag="PLL88_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02174.1"
                     /translation="MFTINASDFTLMLAIVLAAMGLITFFVGIIILAFKVKSDEFSSI
                     TEQSAKLMEKGVTDNITELMGNTTSLLETINQMIKTKAGIGVFLVLVTFILFGIAYYL
                     ITGL"
BASE COUNT         1420 a          905 c         1147 g         1162 t
ORIGIN      
        1 gccaggttgg gatggagttg gaggatcgtg aaaaaagtgt ggctgcggct atcttgacgc
       61 aattggatga agatggattt gtggatgtgg atgtgttgga gctggcaagc tactatcata
      121 ttccgatttc gaacgtggaa aaagtgaaaa gtatgatcca acatgccgat ccaatcgggg
      181 tcggctctca agatgcccgc gaagccatgt tggttcagat cgatgaatta aaaaagaaca
      241 aagaaatccc ggcatttacc gaaaagatta tttcggacca tttttatacc ttgctgaaga
      301 aacaattctc tgagatcgct gaacaaaatg ctatttccat aaaacaggtg gaaaccgtag
      361 ccgactttat cacagagaac ctgaatccat ttccggcgcg ttcgaattgg ggagatgtga
      421 ggaatccaca ggaaaatgat cttttgcaat tgggcgcacc cgatatcatc ttgagttttc
      481 tggataataa ccctcaaaag cagatcatta ttgaggtgtt tcagccggcg aacagcaatc
      541 tggtgattaa cacaagctat catgcggcat taaaaaacgt cgataaagaa acaaaggtga
      601 aaatgaggga ggatcttgaa aaagcatccc tttttatcaa atgcctgcaa caaagaacca
      661 acaccattct tcgcatggcg caggttctgg tggagataca agaaaagtac attcgcgagg
      721 gcgataagta tttaaaaccg atcactcgag cagagatcgc cagaaggctc gatgttcacg
      781 aatccaccat ctcccgcgcg gtttccagca agaccattca attgcccaat agaaaggtct
      841 acccgctctc tgtgtttttc gacaggagtc tccagatacg gacagagata aaagagattg
      901 tgaaaaagga gaaagaacca atgagcgacc tggagattgt gaagaaactg gagaagaaag
      961 gcatgcatat cgcacgcagg acggtggcaa aatatcgaga tatggaaggg atcttacctg
     1021 cctatcaacg taaagacaaa acaaaatgac agcattcatt gaccttatta agaataaaaa
     1081 cctttccttt ttggagatcg aatctcttct gaatttgttc aaccaaccct gtgtgttgtc
     1141 agacgaggat aatgaaattg cgcttgtgaa tagcgctttg atcgaactaa ccgcattcac
     1201 caaacaggag ttgattcgca ctccgttacg atcgttgatc acggaagaac gagcggagaa
     1261 tgggaaagtg gtctatagaa tactgcgcaa aaaccgtatg ccgcttgagg tggaactctc
     1321 gcgggaatat ttggatgata aacaccattg gaaggtctac ctgatcaacc agaaaatgca
     1381 aggcgatgtc attgattaca aagctttgaa atcctacctg accacgatgt cagccctgat
     1441 cgacgctcgt gatgaaacag atgcatcctt ttacgaaaag agtttggata ttctggataa
     1501 agtttttcat tttgacctct tgtgtttcta tgttgaagag aatggagttt ttcagctttc
     1561 cgatctgagc aagaaaacgc cccctcattt tccggaaaaa attttggtca atgaactgga
     1621 aaatatcgat cagtacaatc tgtggcagca gggaaaccgg gctatcaatc atctgcaccg
     1681 ctctgctcga gaacagttca ttaatgttct tgttacccag cgctttaaga aagatcacga
     1741 tcaggaagga ttgattgtcg caggatggaa agagggcgtg agagattcct caatgctggc
     1801 tatcgtagaa gaatatatga aaatggttga acacggcgca tccctgttca accaaaatac
     1861 aattcacgaa caaatacgct ctgaaagcgc aaaagcgcaa gaaatattga gctataccct
     1921 ggaaaatatc gaagaagggg tgatcgttct cgattacgag atgaaggtgc ttgagataaa
     1981 tcagaatatg gaacgtttgc tgggatattc gaaatgggaa gtgcagggat tgtttgtgga
     2041 ttcttatctg gtagcagacc caaagataac cacactattc catgacgttt ttgaaagaca
     2101 aaaggaacaa tgtgaaaacg gggtgtcgtt aaggcaaaga gatggggatg cggagccagt
     2161 gaagatgcgc gtgatccctc gcgatgtcag agatcctcaa acgcagttcc tgaccctggt
     2221 caggtcgacg cgcgagctgg acggattgaa aaggaacctt aaagaattgg aacatcaggc
     2281 tgcgctggga aaatcggtgg ctacttttgc tcatgaagtt cgtaatccta tcaataatat
     2341 ggtaacggga ttgcaggtac tgcaaagcat gtctgaagaa aatgaggcgc aatctgatct
     2401 gataacccgc atgatgaacg attgcgtacg gctggatcat ctcatgaatt cgatcctctc
     2461 gtacgcaaaa cctctggaaa ataaattgaa gccactcaac ctggatctgc tggttaaaag
     2521 cacgatcgaa aagtgggaag caaaactaac gcgtaatgat gtgaaaatga tttaccagtg
     2581 tgaggaagag gcgccggtgg tgatggggga tatgcgagca ttggagcagg tttttacgaa
     2641 ccttatcagt aatgctgtgg aagccatgaa gccacaaaat ggaggtaccc tggcaataaa
     2701 aattgaaaaa tcgcaagcaa aaaccctaac agtaactgtg tcggacaccg gtccgggcat
     2761 tccggatgag atattgaaaa atcttttcac tccatttgtc tcatttagct tgcaaggcac
     2821 cggattgggt cttgccatca taaaagaaat tattgctgcg cacaacggcg aaataaccgt
     2881 tgattcgttt ccgggtggta ctgtgttttc tattgctttg ccaattgcag aaggagagta
     2941 aggtgagctt tacagttctg atcgtagatg atgaagagaa tgcacgcaag aacctgagcg
     3001 tttttcttaa atcaaagaat ttcgaggtct ttgaagcagg gaccctatct gaagccagag
     3061 agcaattgaa gatggggaat ggcgatatta tcttgctgga tgtccaactc ccagatggat
     3121 acgggcaaac gttattgaaa gaactccagc aacagcagaa cagaccgccg gtcattttaa
     3181 ttaccggttt tggggatatt gaaatggcgg tggatgccat gcgcaatgga gctcacgatt
     3241 ttttgacaaa acccatcacg gatttcaaaa ttttggaaaa ttcgcttgcc agagcctgtg
     3301 aattgatcaa aatgcggcga gaaattgagc actatcggca aaaacaactg gactccgtcg
     3361 attttgtgct tggttcgaat gagaagatga aaaatgttgt ggcgcaggca ttaaaggcat
     3421 cgcgcgccat ggtttctgtg ctgatcaccg gcgaaacagg cactggaaaa gaagtgctgg
     3481 ctcgcttcat tcataaatca ggtccgaggg cagaaaaaca attcgtggca ataaactgcg
     3541 cagccatcca atccacattg cttgaatcgg aactgtttgg gcatgatgcc ggcagctata
     3601 cggacgcaaa aacccgcaag catgggctca tggaaatcgc tgatgatggc atcttgttct
     3661 tggatgaaat ttcatccatg ccattggaaa tccaggctaa actgttgcga gctattgaag
     3721 aaaaagcctt tcgcagagtg gggggaaacg acctgctaaa agtggatgta caaatattgg
     3781 ctgcatccaa tcgaaatctg aaagatctga tagcagaaaa agctttcaga gaagatttat
     3841 attatcgcct gaaggtcgtt gacctggact tgcctcctct aagggagcgg aaaaatgaca
     3901 tccctgaatt cgttgggttc ttcataaaag agttaaaccc tcgcctgggt ttgaatgtca
     3961 ttgatgtgac tccagcagcc atggaagtat tgggtaagta ttcatggccg ggcaatattc
     4021 gtgaattgcg caacacgatc gagcgagcca tgctgttctg tgatggggaa aagattgatg
     4081 tggctgattt accgttggat ctggtagaaa aattggcata attattgcaa taatatctct
     4141 ggaaaaggag atattatgtt cacgataaac gcaagtgatt ttacattaat gctggcgatc
     4201 gtcttggctg cgatgggctt gatcactttt ttcgtcggga tcatcattct ggcatttaaa
     4261 gtgaaaagcg atgagttcag ctcgatcacg gaacaatctg ccaagttaat ggaaaaaggt
     4321 gtcacggata acattactga attaatggga aataccacgt ctttactgga aaccatcaac
     4381 caaatgatca aaacgaaagc tgggattggt gtcttcctcg tcttggtcac cttcatcttg
     4441 tttggtattg cctattattt gatcactggt ctatagagta gagaggacca tatgatacct
     4501 gcgaaattcc tcgatctcat cgagaattat ttccatgagg aagagaaaca ggttatcgtt
     4561 gcatgcctgt tgcaggagaa aagagttctc gatgcgctca ctgttctcga ccacagggag
     4621 ggtgttgaga caaa
//
LOCUS       DAOSDS010000147         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_6540_length_4628_cov_4.816313, whole genome shotgun sequence.
ACCESSION   DAOSDS010000147 DAOSDS010000000
VERSION     DAOSDS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6540_length_4628_cov_4.816313"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="PLL88_11220"
     CDS             complement(<1..460)
                     /locus_tag="PLL88_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ02175.1"
                     /translation="MKKSSVIFFILIFLFMGCTSPFSNSHSSASTLSEKQYIENKGSD
                     TLVNLALAWAEAYQLQHPEVQISVSGGGTGTGITALINGTVDIANASRAIKEEEIANA
                     EANGIEPYEIVVANDAIAVIVNPENPVNRLTIEQVSLIYQGIITNWKEVGG"
     gene            complement(557..1960)
                     /gene="cysS"
                     /locus_tag="PLL88_11225"
     CDS             complement(557..1960)
                     /gene="cysS"
                     /locus_tag="PLL88_11225"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOJ02176.1"
                     /translation="MPENKKVLKLYDTYTRSKREFIPLKPTHVGLYCCGPTVYDFAHI
                     GNLRTYLFEDILRRVLEFNGYAVRHVMNITDVGHLTSDADTGEDRMEKGSRRTGKTAW
                     KIAETYTDAFKADLARLNIQAPHIWCRATDHIKEQIDFIQCIESKGFTYRTSDGIYFD
                     TSKIPDYGYLARLHVEGLQAGRRVNVGEKRHITDFALWKFSTPNEKKQMEWKSPWGTG
                     SPGWHIECSAMAVKYLGNFFDIHCGGEDHIMVHHPNEIAQNQACYGTRLANFWMHSSF
                     LQIDNSRMGKSMGNFVVLKDIIALGYDALAWRFYCLNAHYRSKLNFSTEGLDQAKASI
                     TRLRNLSCQWGKAGSTDDHFMAKFQAQVNDDLNTPRAMATVWELTASDLPDSAKKATL
                     LEFDKVLGLGLDDWQPTPEAIPDEVKALAERREFARRSRNWNEADIFRDQIHQLGYEI
                     EDTPDGYQLRMKHKRKP"
     gene            2154..4613
                     /gene="gyrA"
                     /locus_tag="PLL88_11230"
     CDS             2154..4613
                     /gene="gyrA"
                     /locus_tag="PLL88_11230"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOJ02177.1"
                     /translation="MEISGIKRIDIDQEMRTSYIDYAMSVIVARALPDARDGMKPVHR
                     RILYAMMDMGVHSNSSYKKSARIVGEVLGKYHPHGDAAVYDAMARMAQDFSMRYMLVD
                     GQGNFGSIDGDPPAAMRYTEARMGVLADEMLVDIDKHTVDFADNFDGSLQEPLVLPAR
                     LPNLLLNGASGIAVGMATNIPSHNLGELCRTLVYVIDNQDNLDEITVEDLVRYLPGPD
                     FPTGGIIVGSEGIRQAYSTGKGKITLQGKATIEEGKGGRFVIRVTEIPYQINKTTLIE
                     RIAELVREERIPEISDLRDESDRTGMRIILELKRGAQPKRVLNRLYKFTQLQSTFGIN
                     MLALVNGEPRMLTLKHALQIFIDHRMDVIKRRSEFELEKARNRAHILEGLLIALANLD
                     EVIRTIRESKDADVAKTALMKRFKLSEIQAQAILDMQLRRLAALERMKIETEYKEIMD
                     RIAYLEDLLAHPRKMLEVIRNDLQEIGEKYSDRRRTLISDGGVEDLSEEDMVSDVPAL
                     VTITQRGYVKRVSPQVYKTQARGGRGVIGQGMKEEDEVALLVPCRSLHTLLFFSDRGK
                     IYSEKVFQLPEGGRTDKGLSLMNVIALEQGERITAAIAVPNFENAHFLTFATVQGYMK
                     RVELKEFESVRPSGMIAITLGEGDELGWVALTAGKDELILVTANGQALRFNEKEIRPM
                     GRAARGVIGIRFKDGDALTSMAVVEKDASLLVVTEKGYGKCTPLNEYRSAGRGTQGVS
                     TINKDALAKIGKIASARVVQEGDQVTMISTNGLVIKLNVKDITTSGRSTRGVKLMNIE
                     KNDRVATVARFTDSMLNAKEG"
BASE COUNT         1107 a         1126 c         1211 g         1184 t
ORIGIN      
        1 caccgccaac ctctttccag ttggtgatga tgccttgata gattagcgat acctgttcga
       61 tggtcagccg gttaaccggg ttctcaggat tgacgatcac cgcgatggca tcattggcaa
      121 ccacaatctc atagggttcg atcccgttgg cttcggcatt ggcgatctct tcctctttga
      181 tggctcgtga ggcattggcg atatccaccg taccgttgat gagcgcagtg atgccggtgc
      241 ctgtgccgcc accagaaacg gatatctgca cctcgggatg ctgcagttga taggcttctg
      301 cccatgccag cgccaggttg accagggtat cagaaccttt gttttcaatg tactgttttt
      361 cagaaagtgt gctggcggaa gaatgactgt tcgaaaatgg gcttgtgcag cccatgaaaa
      421 gaaagatcaa aataaaaaaa atcacgctgg attttttcat gataaaaatt ataccgtatc
      481 caaagaattg aagaaaaggt cctgatgatc gactggggca agcagtgttg aaagttttat
      541 aagggactca aattcgtcag ggttttcttt tgtgtttcat ccttagttga tagccatcgg
      601 gcgtatcttc gatttcatat cccagttgat ggatttggtc acggaaaatg tcagcctcgt
      661 tccaatttct gctccttcgg gcaaattcgc gtctttcagc aagcgctttc acctcatctg
      721 gaatggcttc tggggtgggt tgccagtcat ctagccctaa tccaagaact ttgtcaaact
      781 ccagtagcgt tgcttttttc gctgaatcag gcagatcgct tgccgtcagc tcccatactg
      841 ttgccattgc acgaggagta tttagatcat cattcacctg tgcctgaaat tttgccatga
      901 aatgatcatc agtgctacca gcttttcccc attgacacga taagtttctc aatcgagtta
      961 tggatgcttt cgcctgatcc aacccttccg tggaaaaatt gagctttgag cgataatgag
     1021 cattcaaaca atagaagcgc caagctaacg catcatagcc cagggctatg atgtccttta
     1081 aaaccacaaa gttccccatc gattttccca tgcgagaatt gtcgatctgt aaaaagctgc
     1141 tatgcatcca gaagttcgcc aaccgcgttc cgtaacaggc ttggttttgg gcgatctcat
     1201 tggggtgatg caccatgatg tggtcctctc ctccacagtg aatatcgaag aagttcccaa
     1261 ggtatttaac tgccattgct gaacattcga tgtgccagcc gggagaaccg gttccccagg
     1321 ggcttttcca ttccatttgt tttttctcat tgggtgtgct gaatttccaa agggcgaaat
     1381 cagttatatg ccgtttttct cctacgttga ctcgtcgtcc tgcctgtagc ccttcgacat
     1441 gcaatcgggc taagtagcca tagtccggaa ttttggaggt gtcaaaatag atgccatcag
     1501 aagttcggta ggtaaatcct ttgctttcga tgcattggat gaaatcaatt tgctctttga
     1561 tgtggtccgt tgcacggcac cagatatgtg gtgcttgaat gttcaagcgg gctaaatctg
     1621 ctttgaaagc atcggtgtag gtttcggcaa ttttccaggc tgttttcccg gttcgtcgcg
     1681 aacctttttc cattcgatct tcgcctgtgt cggcatctga ggtcaaatgc ccgacatcgg
     1741 tgatgttcat gacgtgcctg acagcatatc cattgaattc aagaacccgg cggagaatat
     1801 cctcaaaaag ataggttcgt agattcccaa tatgggcaaa atcatagaca gtgggtccac
     1861 aacaatacaa tccaacgtgt gtgggtttta atggaatgaa ctcgcgttta ctgcgcgtgt
     1921 aggtatcata aagcttcagc acttttttgt tttctggcat aacaaccccg catttcaaag
     1981 agttcccgta tcgatttgcg aacgcgaagc gattgattat tgataacctg attatatact
     2041 tcggcggact gatttgaaca attcttgtgc ctgaaggcga gctagctgac atccttttgg
     2101 gagtaaggta taattatcac tcccgtgaat tgatagagga gttttgtttt taaatggaaa
     2161 tcagcggaat taaacgaatt gacattgacc aggagatgcg tacctcttac attgattacg
     2221 ccatgagcgt gatcgtggcg cgcgcgttgc ccgatgcgcg tgatggcatg aagccggtcc
     2281 atcgccgtat tctctacgcc atgatggaca tgggtgtgca ttccaattca tcctacaaaa
     2341 aatctgcccg tatcgtgggc gaagttctcg gtaaatacca cccgcacggc gacgcggcgg
     2401 tgtatgatgc catggcgcgc atggcgcaag atttctccat gcgctacatg ctggtggacg
     2461 gacagggcaa ttttggttct attgatggcg acccgccagc tgccatgcgc tataccgaag
     2521 cccgcatggg cgtacttgcc gatgaaatgc tggtagatat cgacaagcat accgtggatt
     2581 ttgccgataa ctttgacggt tcattgcaag agcctttggt tctgccagcc cgcctgccca
     2641 atttgctgtt gaacggcgct tcgggcatcg cagtgggtat ggctaccaac atcccttcac
     2701 acaacctggg tgagctatgc cgcaccctgg tctacgtcat cgataaccag gataatctgg
     2761 atgagatcac cgtggaagac ctggtgcgtt acctgcccgg tcctgacttt ccgaccggcg
     2821 gcatcattgt aggctccgaa ggcatccgcc aggcgtacag caccggcaag ggcaagatca
     2881 ccctgcaggg caaggcaacg atcgaagagg gcaagggcgg gcgtttcgtg atccgcgtta
     2941 ccgagattcc ctatcagatc aacaagacca cgctcatcga acgcatcgca gaactggtgc
     3001 gcgaagagcg cattcctgag atctctgacc tgcgcgatga atcggaccgc accggcatgc
     3061 gcattatttt ggaattaaaa cgcggggcgc agcccaagcg ggtgctcaac cgcttgtata
     3121 agttcacgca gcttcaatcc acctttggca ttaacatgct ggcactggtg aacggtgaac
     3181 cccgtatgtt gaccctcaaa catgccctgc agatcttcat cgaccaccgc atggatgtca
     3241 tcaagcgccg ctcggaattt gaattggaaa aagcgcgcaa tcgtgctcat atcctggagg
     3301 gcttgttgat cgcgctggcg aatctggatg aggtcatccg caccatccgt gagtcgaaag
     3361 atgcggatgt tgccaaaact gccttgatga aacgcttcaa actcagcgag atccaggctc
     3421 aagccattct ggatatgcaa ctgcgccggc tggctgctct ggaacgtatg aagatagaga
     3481 cagaatataa agagatcatg gatcgtattg cctatcttga agatctgctg gcgcatcccc
     3541 gtaagatgct ggaagtgatc cgcaacgatt tgcaggagat cggtgaaaag tacagcgatc
     3601 gacgccgcac cctcatttca gatggcggtg tggaggatct ctcggaagaa gatatggtat
     3661 ctgatgttcc cgccctcgtg accatcacgc agcgcggata tgtcaaacgt gtttctccgc
     3721 aggtctacaa aacccaggcg cgcggtggac gcggtgtgat cggacagggc atgaaggaag
     3781 aagacgaagt ggcgctgctt gtgccgtgcc gctcactgca cacgttattg ttcttctccg
     3841 accgcggtaa gatctactct gaaaaagtgt tccagcttcc tgaaggtggt cgcaccgata
     3901 agggtctgtc gctcatgaac gtgattgccc tggagcaggg cgaacgcatc acagccgcca
     3961 ttgcggtgcc taatttcgaa aacgcacatt ttctgacctt tgctactgta caaggttata
     4021 tgaaacgggt ggaattgaaa gagttcgaaa gcgtacgccc ctcgggcatg attgccatca
     4081 ctttgggtga gggggatgaa ctgggttggg tcgccctgac cgccgggaag gatgagctca
     4141 tcctggtgac cgccaatggg caggcgctgc gcttcaatga gaaggagatt cgtcctatgg
     4201 gtcgcgctgc gcgtggcgtg atcggcattc gcttcaaaga tggtgatgcg ctcacttcta
     4261 tggcggtagt ggaaaaagac gcttctctgc tggtggtgac tgaaaagggg tacggcaaat
     4321 gtacgcccct gaacgaatac cgttccgctg ggcgcggtac gcagggtgtt tctacgatca
     4381 acaaagatgc tctcgccaag atcggcaaga ttgccagcgc ccgcgtggtg caggagggtg
     4441 atcaggtgac catgatctct accaatgggt tggtcatcaa gctcaatgtg aaagatatca
     4501 ccacatcggg tcgttcaacg cgcggtgtga agttgatgaa catcgaaaag aatgacaggg
     4561 tcgctactgt tgcgcgcttc accgatagca tgttgaatgc aaaagaaggg tagatatatc
     4621 agctcaaa
//
LOCUS       DAOSDS010000148         4628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_6541_length_4628_cov_4.035863, whole genome shotgun sequence.
ACCESSION   DAOSDS010000148 DAOSDS010000000
VERSION     DAOSDS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6541_length_4628_cov_4.035863"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..395
                     /locus_tag="PLL88_11235"
     CDS             15..395
                     /locus_tag="PLL88_11235"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOJ02178.1"
                     /translation="MSADTKRPQKLISIILVIAFFFMLLGFNDRLSEYFKLSRQRNEI
                     ATEVYGLQSTEIMLRTQIAYASSDHAVEDWARGEAHMALPGDVVIVPLTPSAADGQSS
                     ITPTPTSSSLENWQIWKNYLFGGD"
     gene            465..1154
                     /locus_tag="PLL88_11240"
     CDS             465..1154
                     /locus_tag="PLL88_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ02179.1"
                     /translation="MLDRKNLPNLMGGRNGKKRSMLMTLTILAIILVILGIVMIVLWI
                     AGGGFSFSLFSRRPTPTSTMPPTPVMSPTASLIPTQTFTPEPTITTTPSGPFEYEVQE
                     DDSCWVIAENFDVDFLTLLAINNFEDGTCPIIPGMKILIPAPGQSLPTPTDVPADMKS
                     GTKISYTVQSGDTLASIASKFNTTVEDILAQNKNVIEDQDNIGVGIVLTIRVNLVTPT
                     PTFAPTSTLAQ"
     gene            complement(1242..2381)
                     /gene="queG"
                     /locus_tag="PLL88_11245"
     CDS             complement(1242..2381)
                     /gene="queG"
                     /locus_tag="PLL88_11245"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOJ02180.1"
                     /translation="MNALLEQVTTYCHSFGFSLVGFTTPADMQFFPVYQKWIADGCQA
                     GMQYLAGERALFQRRSPRNVMPNCQSILVLAYPYLTKIFSDQEAEEHFPSVAAYACLE
                     DYHLFLPPLLEKIVQFIQSQVAYPLQSRILTDSGPLLERELAYRAGLGWIGKNSCLIN
                     QEHGSFFFLAEILLDMPLDEPESNPAHPSDRCGTCQRCIDICPTQCIRNDRTLDARKC
                     ISYLTIENKGEIPLSLRPMIGEHLFGCDLCQQVCPWNRKALAASKKIAYLQFLLEYFY
                     KPQPSRILNEMEFNQKFHSSPISRAKRRGLYRNLAVVLGNRKNPRNVPTLLALLNDTD
                     ALVRQHAVWGLGCFLSDMVRFRLQEALQVEKDETVLLEIRRVLSQ"
     gene            complement(2394..3245)
                     /locus_tag="PLL88_11250"
     CDS             complement(2394..3245)
                     /locus_tag="PLL88_11250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ02181.1"
                     /translation="MKKILIPFILIALTLAACNANTATSPTETATIVLPTATPTLQPS
                     ETPTPLPPTATAIPYTGISSPLNNISLDELNSINTNAFDPPPAGFDEPHQGIDYSYFR
                     FQNERMIDNDGIEGLEILSVLDGTVAAVISDRNPYGNAVIIETPLALLPPEWLAAISL
                     PEIAPTLVPDPRHNCPALIDNAEWDTSNRSLYLVYAHMQNAPQLLINQHVSSGEVIGQ
                     VGNTGDSTNPHLHFEIRIGPGGVTFTSMAKYDTRASIEEMANYCTWRISNRFEVLDPD
                     IILQFRP"
     gene            complement(3242..4546)
                     /locus_tag="PLL88_11255"
     CDS             complement(3242..4546)
                     /locus_tag="PLL88_11255"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HOJ02182.1"
                     /translation="MPHKEAYQKPVFYLPKKNVDLQKWAVIACDQFTSQPEYWSAVEK
                     FVGDAPSTYHLILPEAYLETPKAEEHGRKTYELMREYLNQGLFDQYEGFVYIERNFGS
                     KIRRGLLVNLDLEQYDFNQGSQTLIRASEGTILDRLPPRIKIREKALLELPHIMVLID
                     DRQDELFSYLSNNKQNYQKIYDFELMQNSGFLKGFLVDNQGEQKVHHIFTSLGDEHAF
                     KEKYILKGNLPPLIFAVGDGNHSLATAKSIWESIKDHVPADHPARYAMVEIVNIHDES
                     LMFEPVHRVVFNFQGNIFSEMSEYFQNALEIKEVESFSSLQTIVRSALSAQVIGLAQQ
                     KGLYSIRFLNPNTNLVVGTVQQFLDQLLKKASASSLDYVHGDEVLKDLCMKPNNMGFL
                     LPAMQKNDFFRTVILDGSLPRKTFSMGDAREKRFYLECRKIQ"
BASE COUNT         1229 a         1092 c         1158 g         1149 t
ORIGIN      
        1 ggtatcattg aattatgagc gctgatacca aacgccccca aaaactcatc agcatcatcc
       61 ttgtcatcgc gttctttttt atgctcttgg gtttcaatga tcggctctcg gaatacttca
      121 aactctcgag acagcggaat gaaattgcta ccgaagtgta tggtttgcag agcaccgaga
      181 tcatgttgcg cacccagatc gcctatgcgt ccagcgatca cgcagtggaa gattgggcaa
      241 ggggggaagc ccacatggcg ttgccgggag atgtggtgat cgtcccctta acccccagcg
      301 cagcagatgg acaatcgtca attaccccga ccccaacatc ctcgtctctg gagaactggc
      361 agatatggaa gaactacctc ttcggcgggg actgattcgg cttgacaaaa tcaggtacaa
      421 taaggtactt atgctagata aaaaccagaa agaaccagga aaaaatgtta gatagaaaaa
      481 acctaccgaa tttaatgggc ggaaggaatg gaaaaaaacg ctccatgctc atgacgctga
      541 cgatcctcgc gatcattctg gtaatcttgg gcattgtgat gatcgtgcta tggattgcag
      601 gaggaggatt ctctttttcc ttattttcca ggcgacccac tccaaccagc accatgcctc
      661 ccacaccagt catgtctccg actgccagtt taatcccaac acagaccttt actccagaac
      721 caaccattac caccactccg agtggacctt ttgaatatga agtgcaggaa gatgacagtt
      781 gttgggtaat cgcggagaac tttgacgttg atttccttac cttgctggct atcaataatt
      841 tcgaggatgg tacatgcccc atcattccgg ggatgaaaat cctgatcccc gctcccggtc
      901 aatccttgcc gactcccacg gatgtaccgg cagacatgaa atcgggcacc aagatctctt
      961 acacggttca atcaggagac acgctggctt cgattgcaag caaattcaac actacggttg
     1021 aagatatcct ggctcagaac aagaatgtga tcgaagatca ggacaatatt ggggttggga
     1081 tcgtattgac catacgggtg aacctggtaa ctccaacgcc cacctttgca cctaccagca
     1141 ccctggcaca gtaaaacaga agaggccgac tcaaaagaaa gagttgcagt tcaaaaagaa
     1201 cacgaacgaa aaaagaaact gaccttttgg gtcagttttt tctattggct aagaacccgc
     1261 ctgatctcca aaagcaccgt ttcgtctttt tccacctgaa gggcttcttg caagcgaaat
     1321 cgcaccatat ccgaaaggaa acatcccagc ccccaaaccg cgtgttgtcg gacgagagcg
     1381 tctgtatcat tcaataatgc caatagcgtg ggaacattcc tggggttttt tcgattgcca
     1441 agcaccaccg ccagattccg gtagagaccg cggcgttttg cacgcgaaat tggagaagaa
     1501 tggaatttct ggttaaattc catttcgttc aagatgcgag aaggttgcgg tttataaaaa
     1561 tattccagta agaactggag gtaagctatc ttttttgaag cggcgagcgc ctttcgattc
     1621 catgggcaaa cttgctggca aaggtcacag ccaaaaagat gttcgccaat catcggtcgc
     1681 agtgaaagcg gaatctctcc cttgttttcg atggtgagat acgaaatgca cttcctggcg
     1741 tcaagcgttc tgtcattgcg tatgcactgc gtgggacata tgtcaatgca ccgctggcag
     1801 gtaccgcagc ggtcagaagg atgggcaggg ttgctttccg gttcgtccag cggcatatcc
     1861 agcaagatct ctgcaaggaa aaaaaacgat ccatgctcct ggttgatcag gcatgaattt
     1921 ttcccgatcc agcccagacc agcccggtaa gcgagttccc gttccaataa aggacctgaa
     1981 tcggtgagaa tccgactttg caatggataa gccacttgag actgaatgaa ttgaacaatc
     2041 ttctcaagaa gtgggggaag gaagagatgg taatcttcca aacaggcata tgctgcaacg
     2101 gaaggaaaat gctcttctgc ctcttgatcc gaaaaaattt ttgtcagata gggatatgcc
     2161 aaaaccagga tagattggca attcggcatg acattgcgcg gagatcggcg ttgaaaaagc
     2221 gcacgttccc cagccaggta ttgcatccct gcctggcaac catcggcgat ccatttttga
     2281 taaacgggaa agaattgcat atcagccggg gtggtaaacc ccaccaggga aaaaccgaat
     2341 gaatgacaat aagtggttac ctgctcaagc agtgcgttca tttctccatg cgatcatggt
     2401 ctgaattgga ggatgatatc aggatcaagc acctcaaaac ggttgctgat gcgccaggtg
     2461 caataattag ccatttcctc aatgctggcg cgggtatcat attttgccat gctggtgaat
     2521 gtcaccccac ccggcccgat ccgaatctca aaatgcaagt gggggttagt cgaatcgccc
     2581 gtgttgccaa cctgaccaat tacctctccg ctgctcacat gctggttaat gagcagttgc
     2641 ggcgcgtttt gcatatgggc atagaccagg tacagcgaac ggttgctggt atcccactcc
     2701 gcattgtcga tgagggcagg gcaattgtgg cggggatcgg gcaccagggt cggcgcgatc
     2761 tccggcaaac tgatcgcagc cagccattcc ggtggaagga gagcaagagg cgtttcgatt
     2821 atgaccgcgt tcccataggg attgcgatca ctgatcaccg ctgcaaccgt accatccagc
     2881 acagaaagga tctccaaccc ttctatgcca tcgttatcga tcatgcgttc attttgaaag
     2941 cgaaaatagc tatagtcgat gccctggtgc ggttcatcaa aaccagccgg gggtggatcg
     3001 aacgcgttgg tattgatgga gttcaactca tccagcgaaa tgttgttcag tggagagctg
     3061 atgccggtat agggaatggc agttgcggta ggcggtaggg gagttggggt ctcgctgggt
     3121 tgaagcgtgg gggttgccgt tggtagaacg atggtagccg tctcggtagg cgatgtggca
     3181 gtattagcgt tgcaggcagc caaagtcagt gcgatcagaa tgaaaggaat caatatcttt
     3241 ttcattggat tttgcggcac tccagataga agcgtttttc tctggcatca cccatagaga
     3301 aagttttgcg aggcaaggaa ccatccagga tgacagtgcg gaaaaaatcg tttttctgca
     3361 tggcaggcag caaaaaaccc atattgtttg gcttcatgca taagtctttc aagacttcgt
     3421 cgccatgcac gtaatcgaga gatgaggcgg atgctttctt gagcaattga tccagaaatt
     3481 gctgcactgt cccaactacc agatttgtgt tggggttgag aaagcggatc gaatacaatc
     3541 ccttctgctg agccaaacca atgacctgcg cagaaagcgc gctccttacg atcgtttgca
     3601 aagaagagaa agattccacc tctttgatct ctaaggcgtt ctgaaagtat tcgctcatct
     3661 cggagaaaat gttcccctga aaattgaata caacgcgatg aaccggttcg aacatcagac
     3721 tttcatcgtg gatgttcaca atttccacca tggcgtagcg ggctgggtga tcggctggta
     3781 cgtgatcttt gatgctttcc cagatggatt tcgcggtagc cagagaatga tttccatctc
     3841 ccacagcaaa tatcagcggt gggaggttgc ccttcagaat atatttctct ttaaacgcgt
     3901 gctcatcacc caaagaggtg aagatatgat ggaccttttg ctcaccctga ttgtcgacca
     3961 aaaacccttt gaggaaaccg ctgttctgca tcagctcgaa atcgtagatc ttttgataat
     4021 tctgcttgtt gttggataaa taggaaaaca gttcatcctg gcgatcgtcg atcagcacca
     4081 taatgtgggg gagctcaagg agcgcctttt cgcggatctt gatgcggggt ggaaggcggt
     4141 ccaggatggt tccctcactg gcacggatca aggtttggga gccctggtta aaatcatatt
     4201 gttccaggtc aagatttacc agcaacccgc ggcggatttt tgaaccgaaa tttctctcga
     4261 tgtagacaaa tccttcatat tggtcgaaca aaccttgatt gagatattca cgcatcaatt
     4321 cgtaagtttt ccgaccatgt tcttcggctt tgggagtctc cagataggct tctggaagga
     4381 tcagatgata ggtagaagga gcgtccccta caaatttctc aacagcgctc caatactccg
     4441 gttgactggt gaactgatca caagcgatga ctgcccattt ttgcaggtcc acgtttttct
     4501 tgggcaagta aaagaccggt ttttgatatg cttctttatg aggcatggct ttctccagat
     4561 gatgattcct attgtaccaa aaaggactta aaaaaaagaa gccatcgttt gtgatggctt
     4621 ttcatgat
//
LOCUS       DAOSDS010000149         4475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_6850_length_4475_cov_3.316063, whole genome shotgun sequence.
ACCESSION   DAOSDS010000149 DAOSDS010000000
VERSION     DAOSDS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6850_length_4475_cov_3.316063"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /gene="ruvB"
                     /locus_tag="PLL88_11260"
     CDS             complement(<1..878)
                     /gene="ruvB"
                     /locus_tag="PLL88_11260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOJ02183.1"
                     /translation="MNTEAPRIVDPQADDSEQLDNALRPRFLKDVIGQSQIIENLQIL
                     IKASQQRKDALDHVLFYGPPGLGKTTLAHVLANEMRVNIKVSSGPAIERPGDLAAILS
                     NLNGGDILFIDEIHRLGKMVEEVLYPAMEDFALDIVIGKGPSARSIRLKLPRFTVVGA
                     TTRLALISSPLRTRFGAIYRLDYYDPNALKHIVLNAADRLHIQAEEKGVEEIARRSRG
                     TPRVALRLLRRVRDYAQVRAQGVITRDAAGEAMSLLSIDALGLDEIDRMVLDTIIRKY
                     NGGPVGLSTIAASISE"
     gene            961..1587
                     /gene="upp"
                     /locus_tag="PLL88_11265"
     CDS             961..1587
                     /gene="upp"
                     /locus_tag="PLL88_11265"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOJ02184.1"
                     /translation="MENVFVSNHPLISHKLAKLRERTTEPKKFRELIREIAGLLTYEA
                     TRDLELEPMTVETPLEIMQGAILREKIGLVPILRSGLGMVPGVWDLMPSAEVWHIGLY
                     RDEKTLKPVQYYNKLPIEPRVSVCMILDPMLATGGSATATIDVLKNWGVKRIKFVGVI
                     GAPEGIQLVHENHPDVPIYLAAIDRQLNEHGYILPGLGDAGDRQFGTG"
     gene            complement(1639..2406)
                     /gene="tpiA"
                     /locus_tag="PLL88_11270"
     CDS             complement(1639..2406)
                     /gene="tpiA"
                     /locus_tag="PLL88_11270"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOJ02185.1"
                     /translation="MRKPFVAGNWKMNKTVEQASLLVADLLPGLQAIQKIERVLCPPF
                     PALMVLSQMTAGTDIGLGAQNMHWEESGAYTGEVAPAMVKEFCNYVIIGHSERRQYFA
                     ESDATVNLKVKAALKQGLIPIVCVGETVEENEAGRTAEIVKREVLEGLKDLSASDALK
                     LVVAYEPIWAIGTGKAATAELAEEIIGKSVRGSLTERFGADASDQIRILYGGSVKGGN
                     AAEFFTKPDIDGALVGGASLKADDFIQICQAAQDSIQ"
     gene            2547..2895
                     /gene="rnpB"
                     /locus_tag="PLL88_11275"
     ncRNA           2547..2895
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLL88_11275"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2921..3304
                     /locus_tag="PLL88_11280"
     CDS             2921..3304
                     /locus_tag="PLL88_11280"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ02186.1"
                     /translation="MSVIKHFEENADQFQWNSIQPQEITMEGIAGVTKHILIGRDDQS
                     PHYIMRFFWLEPGGHSMLERHPQEHGVIVLKGKGTIQIGEKVSEVHPYDVAFISPDEL
                     HQFNNPYDEPFGFICVIPRLDPHME"
     gene            3327..3599
                     /locus_tag="PLL88_11285"
     CDS             3327..3599
                     /locus_tag="PLL88_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02187.1"
                     /translation="MDIRYLFWAAGGALLGVLYTCSIQQGITDSQKKTMRVNPLFSIM
                     RILFCSAVLIFAFYQGISYGLICLVLFLVFKYLSLSILIKDRNSLR"
     gene            3754..>4475
                     /locus_tag="PLL88_11290"
     CDS             3754..>4475
                     /locus_tag="PLL88_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ02188.1"
                     /translation="MNKRATAKDVADAAGVSRTTVSFVLNNIEGMRISKETRQKVLQA
                     AEALNYHPDASARSMVKGKTFILGFVVRQEPDKAYADLLLPEVMRGISEVAGEHGYHL
                     IFKPVPPADEKNSLTTLFLEHQVDGLIVSGPLSNDQEIVDLFGANAPIILQGRIDYPN
                     IPSVDINNRKAAEKAVNHLVSLGHERIAFISNASLQYSSSKDRLAGYRRALESAGLQY
                     DEALVFYGDRTPDSGYQAMKNIL"
BASE COUNT         1047 a         1274 c         1126 g         1028 t
ORIGIN      
        1 tcttcgctga tggaggcggc aatggtgctc aagcccaccg gtccgccgtt gtatttgcgg
       61 atgatggtat ccagcaccat gcggtcgatc tcatccagac ccagcgcgtc aatgctcaac
      121 aggctcatgg cttcaccggc agcatcacgg gtgatgacac cctgcgcgcg cacctgggcg
      181 taatcgcgca cccggcgcag caagcgcaat gccacccgcg gtgtgccacg cgaacgccgg
      241 gcaatctcct ccaccccttt ttcctccgcc tgaatgtgca ggcgatctgc cgcattcagc
      301 acaatatgct tgagcgcgtt cgggtcgtaa taatccagac gataaatggc accgaagcgc
      361 gtgcgcaaag gagagctgat cagcgccagc cgcgtggtag ctccgactac ggtgaagcgc
      421 ggcaatttca ggcggatgga acgcgccgaa ggtcctttgc caatgacgat atcgagcgcg
      481 aaatcttcca tagcaggata gagcacttct tccaccatct tccccaggcg gtgaatctca
      541 tcgataaaca gaatatcgcc cccgttcaaa ttggagagga tggcagccaa gtcacccggg
      601 cgctcaatgg ctggtccgga agaaaccttg atattgaccc tcatctcgtt cgccagcaca
      661 tgcgccagcg tggtcttgcc caaccccgga ggaccgtaga acagcacgtg gtccagcgcg
      721 tctttgcgct gctgtgaggc tttgatgagg atctgcaggt tctcgatgat ctggctttgc
      781 ccgatgacat ccttcaaaaa gcgcgggcgc aacgcattgt ccaactgttc actgtcatcc
      841 gcttgcggat cgacgatgcg gggagcttct gtgttcatga aaacgattat acctttggat
      901 aagaagcagt ttctgatatt atataggttt gtacgaatta attctaatgg agattggctt
      961 atggaaaatg tctttgtatc gaaccatcct ctcatcagcc ataaactggc aaagctgcgc
     1021 gaaagaacca ccgaacccaa gaaattccgc gaactgatcc gcgagatcgc cgggctgctc
     1081 acctatgaag ccacgcgcga tctggagctg gagcccatga ctgtggaaac cccgctagag
     1141 atcatgcagg gagccatcct gcgcgaaaaa atcggcttgg tccctattct gcgctctggt
     1201 ctggggatgg tgcctggcgt ttgggatctg atgccctctg cagaggtatg gcatatcggg
     1261 ctttatcggg atgagaagac cctcaagccg gtgcagtatt acaacaaact ccccatcgaa
     1321 ccgcgcgtct ccgtctgcat gattctcgac cccatgctgg ctacaggcgg ttccgccacc
     1381 gccaccatcg acgtgttgaa gaactggggc gtgaagcgca tcaagttcgt aggcgtcatc
     1441 ggtgctcccg agggcatcca actggtacac gagaaccatc ccgacgtccc catttacctg
     1501 gctgcaatcg acaggcagtt gaacgaacat ggctacattt tgcccggctt gggcgacgca
     1561 ggcgaccgcc aatttggcac cggatgacaa aaacggctga tcatttcgca gccgtttttg
     1621 tttgaaggca aactcagttt attgtatgga gtcctgagcc gcctggcaga tctggataaa
     1681 gtcatccgct ttcaagctgg caccgcccac cagggctcca tcgatatcgg gcttggtaaa
     1741 gaattctgct gcgttgcccc ctttcaccga accgccatag agaatacgga tctgatcgct
     1801 ggcatcagca ccaaagcgct ccgtcaggct gccgcgcacg ctcttgccga tgatctcttc
     1861 agccagttcg gcggtggcgg ctttgccggt gccaattgcc cagatgggtt cataggcaac
     1921 gaccagtttc agcgcgtcgc tggcactcaa gtctttcaat ccttccaaca cttcgcgttt
     1981 gacgatctca gccgtcctgc cggcttcgtt ctcctccacg gtttcaccca cacacacgat
     2041 ggggatcagc ccctgcttca aggcagcctt taccttcaaa ttgacggtcg catcgctttc
     2101 agcaaaatac tggcggcgtt cggaatgccc gatgatgaca taattgcaaa attccttcac
     2161 catggcgggc gccacttcgc cggtgtaggc gcctgactct tcccagtgca tattttgagc
     2221 gcccagtcca atgtcagtcc cggcggtcat ttgcgatagc accatcagcg cagggaacgg
     2281 cgggcaaagc acacgttcga tcttttgaat ggcttgcaga ccgggcagca gatcagccac
     2341 cagcaaagaa gcctgctcca cggtcttgtt catcttccag tttccagcaa caaaaggttt
     2401 tctcattgat aactccatct gcataaactc gtgcgcttat tataaaacgt ttccgccctc
     2461 cacgctgttt taattgccag tgggtgcctg agttggcatt ctgccgcagt tgacccgctg
     2521 ttccattcac gttatcatac aattgccggg ttggtcgggt gatagcggca gagcctggct
     2581 ctgctgagga aagtccgtac tccacagggc acaaaaccgg ggaaatcccg gggcggggca
     2641 acctgccggt agggccacag aaacataccg cccgcaaggg taagggtgaa aaggtggtgt
     2701 aagagaccac cggctgctgc agcaatgcgg cggtcaggca accccttttg ggagcaaggt
     2761 tgagcaggcg ctgaaatctt ggaacagccc cttccctcac tgcaatgtga gagcgccggg
     2821 tagaccgcac agatagatta tcaccccggt gattccgtca ccggacagaa tgcggcttat
     2881 agatcgaccc ggtattttag caactccagg aggaacaagc atgtcagtca tcaaacattt
     2941 cgaggaaaat gcagatcaat tccagtggaa ttccatccaa ccccaagaga tcaccatgga
     3001 aggcattgca ggtgtcacca agcacatcct gatcgggcgc gatgaccaat caccccatta
     3061 catcatgcgc tttttctggc tcgaaccggg tgggcacagc atgctcgagc gccatccgca
     3121 ggaacacggc gtgatcgttc tcaaaggaaa aggcaccatc cagatcggcg agaaagtaag
     3181 cgaggttcac ccctatgatg tagcctttat ttcccccgat gaactgcacc aattcaacaa
     3241 tccctacgat gaaccattcg gattcatttg tgtgatccca cgcctggatc cccacatgga
     3301 ataaaaggga gttcattttt actcgaatgg atattcgtta tcttttctgg gcagcagggg
     3361 gcgcccttct gggagttctt tacacttgca gcatccaaca ggggatcact gattcacaaa
     3421 agaaaaccat gcgcgtaaat ccgctgtttt ccattatgcg cattctcttt tgcagcgctg
     3481 ttttgatctt tgcgttttat cagggaattt cgtacggatt gatctgtctg gttcttttcc
     3541 ttgttttcaa atatctttcc ttatcgatct taattaaaga cagaaatagc cttcgttaag
     3601 ttttatagtt catgatttca tccgcactcg aagcgatttt ccacgcataa gccagctatt
     3661 tttcatgcaa ccgaaatacc tacttgacat acccttctta ttttcatata atattgctaa
     3721 ctcgttttag caaaacgtgt tagcagcagc cctatgaaca aaagagcaac cgccaaagat
     3781 gtcgcagatg cagccggggt ttcgcgaaca acggtctctt tcgtcttgaa caacattgaa
     3841 ggcatgcgca tcagcaaaga aacgcgccag aaggtgctgc aagccgctga agcgctcaac
     3901 taccaccccg atgcctccgc ccgcagtatg gtcaaaggaa agacctttat ccttggcttt
     3961 gtcgtccgcc aggaacccga taaagcctat gctgatctgc tgctgcctga agtgatgcgc
     4021 ggcatcagcg aggttgccgg cgaacacggc tatcacctga tcttcaagcc cgtcccaccc
     4081 gccgatgaaa agaacagcct gaccaccttg tttctggaac accaggtgga tggtttgatc
     4141 gtctcaggac cgctttccaa cgaccaggag atcgtggacc tgttcggcgc caacgcgccc
     4201 atcattctgc aagggcgcat tgattatccc aacattccct cggtggatat caacaatcgc
     4261 aaagccgctg aaaaagccgt caatcatctg gtcagcctgg ggcatgaacg gatcgcattc
     4321 atttccaacg cttccttgca gtattccagc agcaaggatc gccttgcggg atatcgccgc
     4381 gctctggaaa gcgccggttt gcaatatgac gaagccctgg tcttttacgg agaccgcacg
     4441 cccgactccg gttaccaagc gatgaaaaac atcct
//
LOCUS       DAOSDS010000150         4445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_6905_length_4445_cov_4.341913, whole genome shotgun sequence.
ACCESSION   DAOSDS010000150 DAOSDS010000000
VERSION     DAOSDS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6905_length_4445_cov_4.341913"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..329)
                     /locus_tag="PLL88_11295"
     CDS             complement(120..329)
                     /locus_tag="PLL88_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOJ02189.1"
                     /translation="MSEKEQGTVKWFNGSKGYGFIERDSGEDVFVHYTSIQQEGYRTL
                     TEGQRVEFVVTQGEKGPQAQEVVIL"
     gene            complement(491..1279)
                     /locus_tag="PLL88_11300"
     CDS             complement(491..1279)
                     /locus_tag="PLL88_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOJ02190.1"
                     /translation="MTDLSFLKKMELWLLDMDGTIYLDDHLIEGAENFVNTLIEKQKR
                     FLFLTNNSSKSVRTYIQKLYTLGLEFVSREHVYTSGQAAASYILKTYPRKKVYLVGTS
                     DLQEEFERSGIPFSDEPELVVLGFDTTLTYQKLWRICDLVRAGLPYIATHADVNCPIK
                     DGFMPDIGSVIAFIKASTGREPDVIIGKPNPPILEEISCMFATERSKMVMVGDRLYTD
                     IAMGEKGIRTVLVLSGETSMEDLQMSPFCPDGVVSSVKELVALI"
     gene            complement(1299..2246)
                     /locus_tag="PLL88_11305"
     CDS             complement(1299..2246)
                     /locus_tag="PLL88_11305"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HOJ02191.1"
                     /translation="MYSTIMQSVYQHPELMILDGSSNYNEEGTMPLKVYFDCYPCLLR
                     QVLDAVHYLELDEEITKVILNQSMHDLMTMEDDLTSSEMAAQIHQRVIQNGGGADPYQ
                     NVKRLSLKKALEQCEYAKNLILQSNDPLETALKMCTAGNIIDFGPASDFDLKTALDQA
                     LEMPFSHFDYKEFKSAVEHAERILFLADNAGETVFDRLLIEQINKPMDYVVKSAPIMN
                     DALREDAQLTGFPDYVTIMENGTAIQGTVLSHCSEEFLKKYAMADLIIAKGMANFETL
                     PNEGGRAFFLLKIKCKPIATLSGIPFQSFVVKRGGLLSP"
     gene            2301..3476
                     /locus_tag="PLL88_11310"
     CDS             2301..3476
                     /locus_tag="PLL88_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOJ02192.1"
                     /translation="MSILFRKCQYIIFTLKWLVFSALLIVAILLGACAKTEEATAEGP
                     TAEEAVIEEEAPAEKIKVAFIYIGQPGDLGWTYEHDRGRLMLEEALGDQIETIAVPDV
                     PEGPDAEKAIRDVVEAGAKIVFTTSYGYMDPTAAVAAEYPDVIFEHCSGYLTSDNMAN
                     YFGRIEQPRYLAGIVAGSMTESNFIGYVAAFPIPEVVRGINSFTLGVHSVNPDAEVHV
                     VWTNTWYDPVLEREAAVALLDEGADIIAQHQDTTEPQKAAQEAGKLSIGYDSNMAQFV
                     GDTVLTSPMWNWGVYYISTVQAILDGTWEPHAYWGGMADNVVMLADFSPLVPDDVKAL
                     VEAKQAEFIAGTTDAFCGPITNQAGELVVAEGECISDADQLSMNWFVEGVVGTIPAE"
     gene            3577..>4445
                     /locus_tag="PLL88_11315"
     CDS             3577..>4445
                     /locus_tag="PLL88_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ02193.1"
                     /translation="MTTNKELAVQMRGITKHFPGILANDGIDLDVYSHEILALLGENG
                     AGKTTLMNILAGLYKPDAGQILLKGQEVDFHSPRDAIAHGIGMVHQHFMLVESLTVTE
                     NIILSLKNTGMNLHANQVKKRILELSQQYHLLVDPDASIWQLSVGEQQRVEIMRLLYR
                     GAEILILDEPTAVLTPDEAADLANILRKMAEEGKAVILITHKLNEVTAFSDRVTVLRA
                     GHVVSNLLTRDTTKKELAKLMVGREILFHLEKKKCKAGEVVLGVEHIHAINDKGLPAL
                     IDVSLRICSGEILG"
BASE COUNT         1137 a         1065 c         1041 g         1202 t
ORIGIN      
        1 gtagaacgtc tgcttcccaa gcagaaggtt gtgggttcga gccccatcgc ccgctcaaat
       61 aaaaagagcg gtttgtttct ttcaccgctc ttttctttcc gtagacaaat taattcaaat
      121 tagagaatta caacttcctg cgcctgagga cctttttcgc cttgggttac aacgaactca
      181 acgcgttgtc cttcggtaag ggtacgatag ccttcttgtt ggatagaagt gtaatgtacg
      241 aaaacgtctt caccggaatc gcgttcaatg aatccatatc cctttgatcc attgaaccat
      301 ttcacggtac cctgttcttt ctcggacatg ttttggaact cctttcttag atttcttgag
      361 caagttccaa attaaaagac tactctttct ttctagaact actatttcaa gcacttaatt
      421 ctagcatggc tttttcacgt ttgtctatca tgagtttctt ctttttaacg aaaatatttt
      481 caatccgttc tcagatcaaa gccaccaatt ctttcaccga tgaaaccacc ccgtcggggc
      541 agaaagggct catttgcaga tcttccatgc tcgtctcgcc gctcaaaacc agcaccgttc
      601 ggatgccttt ttctcccatg gcaatatcgg tgtacaggcg atctccaacc atcaccatct
      661 tcgatcgctc tgtagcaaac atgcaactga tttcttctaa aattggtggg ttgggttttc
      721 cgatgatcac atcgggctcc ctgccggtag acgccttgat gaaagcgatc acggaaccga
      781 tatcaggcat aaagccgtct tttatcgggc aattgacatc cgcatgggtg gctatatacg
      841 gtaatcctgc ccgcaccagg tcgcatatcc tccacagttt ttgataggtg agcgttgtgt
      901 caaaccccaa cacgacaagc tccggttcat cggaaaatgg aataccggat cgctcaaact
      961 cttcttgcag gtctgaagtg ccaaccaggt aaaccttttt tctcggatag gttttcaaaa
     1021 tatagcttgc agcagcctgc cctgaagtgt aaacatgttc ccgggatacg aattccaaac
     1081 caagagtata cagcttttga atataggtgc gcacactttt cgaagaatta ttggtgagaa
     1141 acaagaatcg tttttgcttt tcgataaggg tgttcacaaa attctcggcg ccttcgataa
     1201 gatggtcatc cagatagatt gttccatcca tgtctagcag ccataattcc atcttcttta
     1261 aaaaactaag atcggtcatg ggctttaccc gcttcctttc atggtgagag caatccgccc
     1321 ctctttacca caaaactctg gaatggaatg ccgcttaatg tggcaatcgg tttacatttt
     1381 atcttaagca agaaaaaggc tcgtccccct tcgttcggca aggtttcaaa gttcgccata
     1441 cccttggcta tgatcagatc agccattgca tattttttta aaaattcttc cgagcagtgc
     1501 gatagaaccg ttccttgaat ggcagtccca ttttccatga tggtcacata atccggaaag
     1561 cctgttaatt gtgcgtcttc tcttaatgca tcattcataa tcggggcact ttttacaacg
     1621 taatccattg gtttgttgat ctgttcgatc aacaatctgt caaatacggt ttctcctgca
     1681 ttatcagcca ggaagaggat gcgttcagca tgctcaacag cgcttttgaa ctctttataa
     1741 tcgaagtggg aaaatggcat ttccagagcc tggtccaggg cagttttgag gtcaaagtct
     1801 gacgccggtc caaaatcgat gatattcccg gcagtgcaca tcttcaatgc tgtttccaga
     1861 ggatcgttgg attgcaaaat caaattcttt gcatactcgc attgttccag cgctttctta
     1921 aggctcaatc gcttcacatt ctggtaaggg tcagcccctc ccccattttg gatcacgcgt
     1981 tgatggatct gtgctgccat ttcggatgag gtaagatcgt cttccatagt catcaggtca
     2041 tgcatggatt gattgaggat cacttttgtg atttcttcat ccagctccag ataatggacc
     2101 gcatccaata cctgccgcag caaacatggg taacaatcaa aataaacctt cagtggcatc
     2161 gttccttctt cattataatt tgaagatcca tccaggatca tcagttcagg gtgctggtaa
     2221 acagactgca ttattgtact gtacatggtc ttcacatagt tgaaaaacgg ggtaaaaaaa
     2281 ttaaatattt cttaagaggt ttgtctatac ttttccgtaa atgccagtat ataatattta
     2341 cacttaaatg gttagttttc tctgcattac tgatcgttgc tattctgctt ggcgcgtgcg
     2401 caaaaacaga agaagctacg gctgaaggac cgaccgctga agaggcagtg attgaagaag
     2461 aggctcctgc ggaaaagatc aaggtcgcct tcatttatat tggacaacca ggggatctgg
     2521 gttggaccta tgaacatgat cgcggtcgac tcatgcttga agaagcctta ggcgaccaga
     2581 tcgaaaccat cgctgtccct gatgttcctg aaggacccga cgccgaaaaa gccattcgag
     2641 atgtggtaga agctggtgca aagatcgttt tcacaaccag ctacggctac atggatccaa
     2701 ccgccgccgt ggctgcggaa tatcccgatg tgatttttga gcactgctcg ggttatctca
     2761 catcggataa catggcaaat tattttggac gcatcgaaca gcctcgctat ctggcaggga
     2821 tcgtcgccgg aagtatgacc gagtcgaact tcatcggtta cgtagcagct ttccccatcc
     2881 cagaagtagt tcgcggcatc aattccttta cgcttggtgt gcacagtgtg aaccctgatg
     2941 ccgaagtgca tgtggtttgg actaacacct ggtacgatcc tgtgttggag cgcgaagcgg
     3001 cagttgccct gctggacgaa ggcgccgata tcattgccca acaccaagac acaactgaac
     3061 cgcaaaaagc tgcccaggaa gctggcaagc tgagcatcgg ctacgattcg aacatggcgc
     3121 aattcgttgg cgataccgtt ctcaccagcc caatgtggaa ctggggtgtg tactatattt
     3181 ctacggtcca ggcaattctg gatggaacgt gggaaccgca cgcgtattgg ggcggtatgg
     3241 cagataacgt cgtgatgctg gcggatttca gcccgctcgt tccagatgac gtaaaagctc
     3301 tggttgaggc gaagcaagcc gaattcattg ctggtacgac tgacgcgttc tgcggaccga
     3361 tcacgaacca agccggtgag ctggttgttg ctgagggtga atgcatcagc gacgcagatc
     3421 aattgagcat gaattggttt gtggagggtg tggtcggcac gattccggct gaataaaact
     3481 tttgctttgt ttaggtttag cccccagggg atggaccaac cttctgctgg gggcttcttt
     3541 atataagcga tcaacggatc gaatcggaac agaattatga cgacaaacaa agaactcgcc
     3601 gtgcaaatgc gcgggattac caaacatttt ccaggaattc tggcgaacga cggcatcgat
     3661 ttggacgtct attcgcacga gatcctggcg cttttaggag agaacggcgc cggcaaaact
     3721 accctgatga acattctggc tggtttgtac aaaccggatg ccggtcaaat tctcctcaaa
     3781 gggcaggagg ttgattttca ctcaccccga gatgccatcg cgcatggcat tggcatggtg
     3841 caccaacatt tcatgcttgt cgaaagctta accgtaacgg aaaacatcat cctcagtcta
     3901 aaaaacactg ggatgaacct gcacgccaac caggtaaaaa aacgtattct ggagttgtcg
     3961 cagcagtatc atctgctggt cgatcccgat gcctccatct ggcagttatc ggttggcgag
     4021 cagcagcgtg tggagatcat gcgtctgctt taccgtggcg ctgagatctt aatcttggat
     4081 gagccaaccg cggttctcac tcccgatgaa gcggcagatc tagccaatat cctcagaaaa
     4141 atggcagaag agggcaaggc ggtcatcctc attactcaca agttgaatga ggtcaccgca
     4201 ttttctgacc gggttaccgt cttgcgcgcc gggcacgttg tttccaacct tttgaccaga
     4261 gacaccacca aaaaagaact ggcaaaactg atggtcggga gagagattct cttccatctt
     4321 gaaaagaaaa aatgcaaagc aggcgaggtt gttctgggag tcgagcacat tcatgccatc
     4381 aatgacaagg ggcttccggc attgatcgat gtgagtctgc gtatctgctc tggagagata
     4441 ttggg
//
LOCUS       DAOSDS010000151         4396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_7011_length_4396_cov_4.609767, whole genome shotgun sequence.
ACCESSION   DAOSDS010000151 DAOSDS010000000
VERSION     DAOSDS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7011_length_4396_cov_4.609767"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..703)
                     /gene="rpsC"
                     /locus_tag="PLL88_11320"
     CDS             complement(26..703)
                     /gene="rpsC"
                     /locus_tag="PLL88_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOJ02194.1"
                     /translation="MGRKVHPIGFRLGINQPWEGRWYAEGQNYIDQLHQDFKIRGLVR
                     KMAEKAGVSFVEIERFPSKIKIVVNTAKPGILIGRKGETVKGIRKSLEELTGKKIDLE
                     IKEIVNPDCDARLVAENIAGQITRRISYSRAMKRAIQQAIRQGAEGVKVEVAGRLNGA
                     EMSRSVWLREGRVPLQTLRANIDFAREHALTAYGQIGIKVWIYKGEIMPGTEEPMEET
                     EGVYVSE"
     gene            complement(724..1059)
                     /gene="rplV"
                     /locus_tag="PLL88_11325"
     CDS             complement(724..1059)
                     /gene="rplV"
                     /locus_tag="PLL88_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOJ02195.1"
                     /translation="MDVRAELKYCPLSPQKVRLVIDVVRGQQVEKAITALKFMQKAAS
                     EPVRKLIESAIANAENNFGIEREKLFIREISADAAPIRKWRRFGARGRFKPILRRSSH
                     VTVVLQEKA"
     gene            complement(1069..1347)
                     /gene="rpsS"
                     /locus_tag="PLL88_11330"
     CDS             complement(1069..1347)
                     /gene="rpsS"
                     /locus_tag="PLL88_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOJ02196.1"
                     /translation="MSRSLKKGPFVDPKLLKKIEEMNTKNEKKVVRTWSRASTIFPQM
                     VGHTIAVHDGRRHVPIYVTENMVGHKLGEFAPTRHFRGHMSEKSASRE"
     gene            complement(1377..2213)
                     /gene="rplB"
                     /locus_tag="PLL88_11335"
     CDS             complement(1377..2213)
                     /gene="rplB"
                     /locus_tag="PLL88_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOJ02197.1"
                     /translation="MSVKIYKPVTPGQRHRAGLTFEEITKTTPEKNLVVARSKKSGRN
                     MYGRITVRHRGGGNKTKVRVIDFKRAKIGIPAVVSAIEYDPNRSARLALLTYADGEKR
                     YIIAPLGLKVGDRVMSGSNAEIKPGNALPIANIPIGTMVHNVELTEGRGGQIVRSAGA
                     AAQLLAKEGEYAQLRLPSGEVRLVRQKCTATIGQVGNLEHSQMQLGRAGQKRHKGIRP
                     TVRGSAMTPRDHPHGGGEGRSPIGMAGPKSPWGKPTLGYRTRKNKKTDKYIVRRRHAA
                     KR"
     gene            complement(2210..2515)
                     /gene="rplW"
                     /locus_tag="PLL88_11340"
     CDS             complement(2210..2515)
                     /gene="rplW"
                     /locus_tag="PLL88_11340"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOJ02198.1"
                     /translation="MKTPFDILKRPLVTEKTAYQSGKLHQYVFEVDGNATKVDIRDAV
                     EKAFNVHVQNVNIINVPAKRGRRARSRRVAIRNSAYKKAIVTLNAEDRIAMFEGVEE"
     gene            complement(2517..3149)
                     /gene="rplD"
                     /locus_tag="PLL88_11345"
     CDS             complement(2517..3149)
                     /gene="rplD"
                     /locus_tag="PLL88_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOJ02199.1"
                     /translation="MKVDVIDFKGKKVGDVELPKDVFEAPINIDLMHQAYVRQMANAH
                     LGTHETKTRGEVAGGGKKPWRQKGTGRARQGSIRAAQWVGGGKIFTPHMRSYDKRMPL
                     KMRRAALRSALSAKASEGEIIVVEDVNLEEPKSKQLSQAFQKWTGDSTALLVMPDKKK
                     ENVFISAKNLRDVKVLLAQYLNIRDLLTYDRLVLEKSSIKVIADYLGKGK"
     gene            complement(3192..3821)
                     /gene="rplC"
                     /locus_tag="PLL88_11350"
     CDS             complement(3192..3821)
                     /gene="rplC"
                     /locus_tag="PLL88_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOJ02200.1"
                     /translation="MFKGLIGKKVGMTQVFDSDGNAVPVTVIEAGPCFVTQIRRVERD
                     GYSAVQLGYEEVSEQKLSGGEKGHLKNREVPAVRYLREFRAKTIDVKEGDKIDVSQFT
                     VGNHVDVVGFSKGRGFAGSVKRYGFRGGPKTHGASDRLRAPGSSGAGTTPGRVYKGTR
                     RPGRMGNDRVTSQNVAVAFVDVEKNLLGINGSVPGPKGGLVVIKEARKQ"
     gene            complement(4026..4334)
                     /gene="rpsJ"
                     /locus_tag="PLL88_11355"
     CDS             complement(4026..4334)
                     /gene="rpsJ"
                     /locus_tag="PLL88_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOJ02201.1"
                     /translation="MAKQKIRIRLKAYDHRVLDQSAKRIVETAERSGAKVVGPVPLPT
                     KIEKFTVRRSSFIDKDSHEHFEIRTHNRIIDVLEPDAKTIDMLMRLNLPAGVDIEIKI
                     "
BASE COUNT         1046 a         1173 c          848 g         1329 t
ORIGIN      
        1 cctctttcca gtacgatacg ccaggttatt cgctaacgta aacgccttca gtttcttcca
       61 ttggttcttc agtgcctggc atgatctcgc ctttgtatat ccaaaccttg ataccaatct
      121 gaccatatgc cgtcagagca tgctcacgag caaaatcaat atttgcgcgt aaggtctgta
      181 gcggcactct cccttcacga agccataccg agcgtgacat ttcagcaccg ttcaagcgac
      241 ccgccacctc caccttgaca ccttcagcgc cctggcggat agcttgttgg atagctcgct
      301 tcatggcgcg gctatagcta atgcggcgcg tgatctgccc ggcgatattc tcggcgacca
      361 atcgagcatc acaatcagga ttaacgattt ccttaatttc caggtcgatc ttctttccgg
      421 taagctcttc aagactcttt cgaatacctt ttaccgtttc acccttgcga ccgatgagaa
      481 tacctggttt ggcagtgttt acaacgatct tgatcttact gggaaatctt tcaatctcta
      541 cgaaagagac ccctgctttt tcagccatct tgcgtaccaa tccgcgaatc ttgaaatctt
      601 ggtgcaattg atcgatataa ttttgtcctt cagcatacca gcgcccttcc cacggctggt
      661 tgattcctaa acgaaatcca attggatgaa cttttcgacc cataatttct cctactcttt
      721 cttttatgcc ttttcctgca gaacgaccgt tacatgagaa gaacgtctca agatcggttt
      781 gaatcttcca cgggcaccga accgccgcca ttttctaata ggagccgcat ctgcggagat
      841 ttcacgaatg aacaattttt cccgttcaat tccgaaatta ttttcagcat tggctatggc
      901 tgattcaatc aacttgcgaa ccggttctga agccgctttt tgcatgaatt tcagggctgt
      961 gattgctttt tcaacttgct gtccacgtac cacatcaatc acaagccgaa ccttctgcgg
     1021 agacaatggg caatatttca attcagctcg tacgtccatg atttcatcct attctctact
     1081 cgcagatttt tctgacatat gaccgcgaaa atgccgtgtg ggagcaaatt caccgagttt
     1141 atgaccaacc atgttttctg taacatagat tggtacatgg cggcgcccat cgtggaccgc
     1201 aattgtatga cctaccattt gagggaagat cgtacttgca cgactccacg ttcgcaccac
     1261 cttcttttca ttcttggtgt tcatttcctc aatttttttc agtaattttg gatctacgaa
     1321 cggtcctttt tttagcgatc gagacatttt tccaagctcc ttaatcttcc actcatttat
     1381 cgcttcgcag catgtctgcg gcgaacgata tacttgtcgg ttttcttatt ctttcttgtc
     1441 cggtaaccca gagttggttt accccaagga gattttggac cggccattcc gataggcgaa
     1501 cgaccttccc caccaccatg cgggtgatca cggggagtca tcgcacttcc gcgcaccgtc
     1561 gggcgaatac ccttgtgtcg tttttgccct gcacgaccaa gttgcatttg gctgtgttca
     1621 aggttgccaa cctgacctat tgtggcagta cacttttgac gaacaagccg tacttcacca
     1681 gagggtaagc gcaattgcgc gtactctcct tctttagcca gcaattgggc agcggcaccg
     1741 gctgagcgga ctatctgtcc gcctcgcccc tcggttagct ccacattatg gaccatggtg
     1801 ccgataggaa tattggcaat tggtaaagca ttacccggtt tgatctcggc attgcttcca
     1861 gacataacgc gatcacctac ttttagaccc aagggggcaa tgatgtagcg tttttcacca
     1921 tcagcatagg tcagcaaagc taagcgggca gatcggttag gatcatattc aatcgcacta
     1981 acgacagcgg gaattccaat ttttgctcgt ttgaaatcaa taacacggac cttggtcttg
     2041 ttaccacctc cgcgatgacg cacggtgata cgcccataca tgttgcgccc tgatttttta
     2101 ctgcgcgcaa caaccaaatt cttttcaggt gtggtcttcg tgatttcttc aaaagtcaat
     2161 cccgctcgat ggcgctgacc gggagtaact ggtttataga tttttacact cattcctcaa
     2221 ctccctcaaa catagcaatt cgatcttcag cgttcagcgt tacaatcgct ttcttatatg
     2281 cagagtttcg tattgcaacc cgacgactgc gcgctcttct tccgcgtttt gctggcacgt
     2341 taatgatatt gacattttga acatgcacat taaaagcctt ctccaccgcg tctcttatat
     2401 caactttggt tgcattacca tccacttcaa aaacatattg atgcaattta ccggattgat
     2461 aagcagtttt ttctgtgacc agtggtcgct ttaaaatatc gaatggtgtt ttcatcctat
     2521 tttccttttc ctaaataatc tgcaatcact ttaatggatg atttttccaa gaccaagcga
     2581 tcataagtca aaagatcgcg aatattcaag tactgtgcga gcaaaacttt aacatcccgc
     2641 aagttcttgg cagaaataaa cacgttctct tttttcttat cgggcataac aagcaatgca
     2701 gtgctatcac ccgtccactt ctgaaatgcc tgggataact gcttgctttt cggttcctcc
     2761 aggttaacat cctcaaccac gatgatctca ccttcgctgg ctttcgcact taatgctgac
     2821 cgtaaagccg cgcggcgcat tttcaatggc attcttttgt cgtaactacg catatgcggg
     2881 gtgaagatct taccgccacc aacccactga gctgcacgga ttgaaccctg gcgcgccctg
     2941 ccagtacctt tctgcctcca tggtttcttg ccgccaccgg caacttctcc gcgcgtcttg
     3001 gtttcatggg tacctaaatg ggcatttgcc atttgtcgaa cataggcttg atgcatcaaa
     3061 tcaatattga tcggggcttc aaaaacatct tttggaagct ctacatcgcc tactttcttt
     3121 cctttgaaat caattacatc taccttcatg attggctcca tttaagcgga atctcgcatt
     3181 ccgaattttc tctactgttt ccttgcctct ttgataacaa ccaaaccacc tttggggccc
     3241 ggaacagatc cattgatccc tagaaggttt ttttcaacgt ccacaaacgc caccgcaaca
     3301 ttttgcgatg ttacacgatc attgcccatg cgtcctggtc tgcgagttcc tttataaaca
     3361 cgcccgggtg tagtacctgc accgctggaa cctggtgctc ttaaacgatc agatgcaccg
     3421 tgcgtttttg gtcctccgcg gaaaccatag cgtttcacag aacctgcaaa tccacgacct
     3481 ttgctaaaac caaccacatc gacatgatta cccacagtga attgactcac gtcgatctta
     3541 tcgccctctt tcacgtcgat cgtttttgcc cgaaattctc tcaaatagcg aaccgccgga
     3601 acctcacgat ttttcaggtg acctttttct ccgccgctca atttttgttc ggaaacttct
     3661 tcatatccca gttgaactgc cgaatatcca tctcgttcaa cccgccgaat ctgagtaaca
     3721 aaacatggcc cagcttcgat tactgtaacc ggaaccgcat tgccatccga atcgaaaacc
     3781 tgggtcatgc caactttttt cccaataagc cctttgaaca tattttgttt ttctccttaa
     3841 aatttgttat caagactgtc agcttgggca aagctgcatc atctttcagt gatgtagtca
     3901 ataatctctt agccaacttg gacttttttc agtcgctaac aagaaaattc ttgcatcacc
     3961 ttttgggtgt tgcccaaaac cgaattaata taccataaat tgataaaatt taccagttct
     4021 cgaatctaga tctttatctc gatatcaacg cctgctggta aatttaacct catcaacata
     4081 tcgatcgttt tggcatctgg ttccagaaca tcaatgattc gattgtgcgt gcgaatttca
     4141 aagtgttcat gagaatcttt atcaataaat gatgaccttc tcacggtaaa tttttcaatt
     4201 ttcgtcggca acggcacagg acccaccact tttgctccgc ttctctcagc ggtttctaca
     4261 attctttttg cagattgatc aagtactcga tggtcgtatg ctttcaatcg gatgcgaatt
     4321 ttttgctttg ccatgatttt ctactccaag atttcagtga tgacgccagc gccgacggtg
     4381 agaccgccct cgcgga
//
LOCUS       DAOSDS010000152         4393 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_7017_length_4393_cov_4.626787, whole genome shotgun sequence.
ACCESSION   DAOSDS010000152 DAOSDS010000000
VERSION     DAOSDS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7017_length_4393_cov_4.626787"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="PLL88_11360"
     CDS             complement(<1..887)
                     /locus_tag="PLL88_11360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOJ02202.1"
                     /translation="MVTPVLEREKESRNYGQFLISPLERGYGVTIGNALRRVMLSSLE
                     GAAVTSIRISDTLHEFTDIPGVREDVLQFILQVKQIRIIMHEGDIAHLHLDVKGEGVV
                     TAADIQVPSEVEIVNPELYLFTVDDPKAELAVDMTVERGRGYSPASTRSGDKSIGELP
                     VDAIYSPVAKVGFDVSNARVGQSTNYDKLTMDIWTDGTITPEQALGNASKILMEQLSL
                     ISGIEEELLEVSKEPEVAVVDHLRSEAADTPIENLDLSVRVFNSLKRTGITSVGDVLE
                     LLDKGEEAVMSIRNFGEKS"
     gene            complement(937..1575)
                     /gene="rpsD"
                     /locus_tag="PLL88_11365"
     CDS             complement(937..1575)
                     /gene="rpsD"
                     /locus_tag="PLL88_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOJ02203.1"
                     /translation="MARYTDSVCKLCRREGEKLFLKGERCYTPKCALEKRAYAPGQHG
                     RTTQGRQGRLSDYSRQLRAKQKARQIYGVLEKQFRRYFSVAIKKRGLTGLNLLQTLEM
                     RLDNVIYRMGLAENRPQARQMVSHGHFIVNGKRTDIPSMLLEPGDVIVVRPQSKDNGL
                     FKDLAERAEKKNCALWIERDLKTFTGRVLRYPERGEIDGNLDEQLIVEYYSR"
     gene            complement(1593..1985)
                     /gene="rpsK"
                     /locus_tag="PLL88_11370"
     CDS             complement(1593..1985)
                     /gene="rpsK"
                     /locus_tag="PLL88_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOJ02204.1"
                     /translation="MAQPSRKSNVKKTKRSLSSATVHVFASFNNTIITVSDSQGNTVC
                     WSSSGAVGYKGSRKSTPFAARMAAEEAMKKAVAMGVDQIDLIIKGPGPGRESAIRAIQ
                     SMGIKVTSIEDITPVPHNGCRPPKKRRV"
     gene            complement(2004..2393)
                     /gene="rpsM"
                     /locus_tag="PLL88_11375"
     CDS             complement(2004..2393)
                     /gene="rpsM"
                     /locus_tag="PLL88_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOJ02205.1"
                     /translation="MARIEGVDLPRNKRVEIGLQYIYGIGPKTAKTILDATKISSDIR
                     VKDLTENDVSALRDYIGKNYKVEGDLRREVQMNIKRLVEIGCYRGLRHRRSLPVHGQR
                     TRTNARTRKGPKHTVAGRGRRRGAAKK"
     gene            complement(2400..2513)
                     /gene="rpmJ"
                     /locus_tag="PLL88_11380"
     CDS             complement(2400..2513)
                     /gene="rpmJ"
                     /locus_tag="PLL88_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOJ02206.1"
                     /translation="MKVSPSIRKRCAKCKIVKRKRVLYVICENPKHKQRQG"
     gene            complement(2573..3229)
                     /locus_tag="PLL88_11385"
     CDS             complement(2573..3229)
                     /locus_tag="PLL88_11385"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOJ02207.1"
                     /translation="MAVFLVLLGPPGAGKGTQAEIISEKLSIPHISSGDLFRENLKNN
                     TELGKQARAFIDSGKLVPDDITISMVAERLSRPDCNSGALLDGFPRTPFQANELDKIL
                     KEKGSAIRCVPYIVVSDRDLLERLTGRWTCKAAGHIYHDTFNPPKNPGFCDIDGSPLY
                     QREDDRRETIEKRLTVFHEQTAPLIDFYREKNLLVEVDGTQPIEKVSEALLKTVRAFV
                     "
     gene            complement(3238..>4393)
                     /locus_tag="PLL88_11390"
     CDS             complement(3238..>4393)
                     /locus_tag="PLL88_11390"
                     /inference="COORDINATES: protein motif:HMM:NF012564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HOJ02208.1"
                     /translation="SGGTLSNFSILAMGVYPYITAQIIIQLLVPLIPSLQKRIQDNPK
                     EGRKLTEKYTILLTIPLSALSAFGQIQIFRQLAGTSPLFTYDFGLKGENIFPTLTTII
                     SMMGGTMLGIWLGELISEYGIPNQGLSLIIFAGIVSRIPQSVYQIITSGENVALLLSI
                     MLTILVVSIFAIVYVQQGQRKVPVIFPGRRVGNRMSMPVRSSLPLMINMAGMIPLIFA
                     QTILSLPAILSQFFINSKTEWVANLFAGVSTVFTAGSVWYVLMYFLLVVGLTFFYTEV
                     LFEQQNYGENLKKQGAQIPGVVRGEPTQRYLMKVQRRITLVGALFLAVVATAPYITQL
                     FLKEGIASQSGLFLVTTSGLLIVVGTVRDTFNVIETELKLHGYEDKLIKG"
BASE COUNT         1167 a         1105 c          885 g         1236 t
ORIGIN      
        1 aaactcttct caccgaaatt tcgaatagac ataacagctt cttcgccttt atccagcaat
       61 tccaatacat cccccacgct agtgatccca gttctcttaa gcgagttgaa aacgcgcact
      121 gacagatcaa gattttctat tggcgtatca gcagcctcac tgcgcagatg atcaacaacg
      181 gctacttctg gctctttgga cacttctagc aattcctctt caatacctga aatcagactt
      241 aactgttcca tcaatatctt tgaggcattt cctaatgctt gttctggggt aattgtccca
      301 tccgtccaaa tatccatagt gagcttatcg taatttgtgc tttgcccaac gcgagcatta
      361 gaaacatcaa accccacttt cgccaccggg ctgtagattg catcaactgg caattcacca
      421 atagatttat cgccactgcg tgttgaggca ggcgaatatc cacgccccct ttccactgtc
      481 atgtccacag caagctctgc tttaggatca tctaccgtaa aaaggtataa ctctggattc
      541 acgatctcaa cttcggaggg aacctggata tctgccgccg tcaccacccc ctcgcctttc
      601 acatccaggt gcaagtgggc aatatcccct tcatgcatga tgatccgaat ttgcttgacc
      661 tgcagtataa attgcagaac atcttcacgc actcctggaa tatctgtgaa ttcatgcagc
      721 gtatcagaaa tgcgaatcga tgtaaccgca gctccttcca aagacgaaag cattacccgt
      781 cttaacgcat ttccgatggt aaccccataa ccacgctcca gggggctgat caaaaactgt
      841 ccataattac gggattcttt ttccctttcc aaaactggag taaccatttt tgtatgagcc
      901 acagcacttc cctcttcctt ttatgtattg atcgttttac cttgagtagt attcaacaat
      961 caattgttca tccaggtttc catcgatttc gccacgttct ggataccgta aaaccctacc
     1021 agtaaaggtt ttcaaatccc tttcaatcca aagcgcacaa ttctttttct cagctctttc
     1081 tgcaagatcc ttgaataaac cattatcctt tgattgtggt ctaaccacaa tcacatcacc
     1141 aggttccaaa agcatggagg ggatgtcagt tcttttacca tttacgatga aatgaccatg
     1201 tgaaaccatt tgacgcgctt gcggtctgtt ctctgccaac cccatgcggt agatgacatt
     1261 gtcaagacgc atttccaggg tttgcagcag gtttagacca gtcaaaccac gcttcttgat
     1321 tgcaacagag aagtaacggc ggaattgctt ttccaacaca ccatatatct ggcgggcttt
     1381 ttgtttcgcg cgcaattggc gactataatc agataagcgt ccttgcctgc cttgcgtagt
     1441 acgcccatgt tgccctggtg cataggcacg cttttcgagg gcacatttcg gcgtatagca
     1501 gcgctcgcct tttaagaata atttctcgcc ttcccttcgg cataatttac atactgagtc
     1561 agtatatcga gccattcttt caatctccta gactatacgc gtcgtttctt cggtggacga
     1621 cagccattgt gcggaacggg agtaatatct tcgatcgatg ttacttttat tcccatggat
     1681 tgaattgcac ggattgcaga ttcacgacca ggaccaggcc ctttgatgat gaggtcgatc
     1741 tgatcaacac ccatggcaac tgccttcttc attgcttcct cagcagccat tcgcgcagcg
     1801 aacggggtgc tttttcttga gcctttatac cctaccgcac cggaacttga ccagcaaaca
     1861 gtgttccctt gtgagtcaga aacggtaatg atcgtattat tgaacgatgc aaacacgtgc
     1921 actgttgccg aagataaact tcgctttgtt ttctttacat ttgatttgcg tgaaggttga
     1981 gccatatttc ctcttgattc ctcttatttc tttgcagctc cacggcggcg tccgcgaccg
     2041 gcgacggtat gctttggacc ctttcgggtg cgagcattcg tgcgcgtgcg ctgcccatga
     2101 acaggtaaac tgcgacgatg gcgcagccct cgatagcaac caatttcgac caagcgcttg
     2161 atgttcattt gaacttcacg gcgcaaatca ccttcgactt tataattttt tccaatataa
     2221 tcgcgcagag cactgacatc gttttcggtc aaatctttca cacgaatatc agaactgatc
     2281 ttggtagcat ccagaatagt ttttgccgtt tttggaccaa taccatagat gtactgcaat
     2341 ccaatctcga cccttttatt tcggggtaaa tcaacacctt caattctcgc catatttttc
     2401 tatccctgtc tttgtttgtg tttcgggttt tcacaaatta catataaaac gcgttttcgt
     2461 ttcacaattt tacatttcgc gcatcgttta cgaatagacg gtgatacttt catctaaact
     2521 cctttacttc cgcaaagttt ttattatact caacaaatta cattccgtcc aatcacacaa
     2581 atgctcgaac ggtcttcaat aaggcttcgc ttaccttctc gatcggctgc gttccatcaa
     2641 cctcaaccaa aaggttcttt tcacgataaa aatcaatcag tggtgcggtt tgttcgtgaa
     2701 aaacagtcag ccttttttcg atggtttccc gcctatcatc ttctcgttga tagagaggcg
     2761 atccatcgat atcacagaaa ccaggattct ttggagggtt gaacgtatca tgatagatgt
     2821 gcccagcagc cttgcatgtc catctgcccg ttaatcgttc aagcaaatcc cgatcggata
     2881 ccacaatata cggtacacaa cgaattgcac tccctttttc ttttaagatc ttatccaatt
     2941 cattcgcttg aaacggagtg cgagggaagc catcgagtag tgcacctgaa ttgcaatcag
     3001 gtctcgataa tcgttcagcg accatggaaa tagtgatatc atcgggaacc agttttccac
     3061 tatcgatgaa cgctcgagct tgtttaccaa gttcagtgtt atttttaagg ttttcgcgaa
     3121 aaaggtcacc cgaagaaata tggggaatag aaagtttttc cgaaatgatt tcggcttgcg
     3181 ttccctttcc cgctccagga ggacctaata agaccagaaa aactgccatt ccgttactta
     3241 acccttgatt aatttgtctt cataaccgtg cagctttaac tcagtttcaa taacattgaa
     3301 tgtgtcgcgc acggtgccaa ccacaatcag caaaccagaa gtggtaacca ggaacagacc
     3361 tgattgtgaa gcaatgcctt cttttaagaa aagctgggta atataaggag ctgtggctac
     3421 caccgccagg aatagagctc caaccagggt tatcctgcgt tgaactttca tcagataccg
     3481 ttgggtcggt tcaccgcgca caacacctgg gatctgggct ccctgttttt tgagattttc
     3541 accataattc tgttgttcaa aaagtacttc cgtgtagaag aacgttaacc ctacaacgag
     3601 caaaaagtac atcagtacat accaaacact gcctgcagtg aacacggtag aaacaccggc
     3661 aaacaggttt gctacccatt cagttttcga gttaatgaag aattgagaca ggatagcagg
     3721 caatgaaagg atggtctgtg cgaagatcaa tgggatcatg ccagccatgt tgatcatcag
     3781 cggaagactg ctccgcacag gcattgacat tctatttcca acgcgcctcc cagggaagat
     3841 tacaggcact tttctctgcc cttgctgcac ataaacgatc gcaaaaatgg agaccacaag
     3901 gatcgtcaac atgatcgaga gcagcaacgc cacattctca cccgaggtaa taatctgata
     3961 aacgctctgt ggaatccgag aaacgatacc ggcaaaaatg atcagagaaa gaccctggtt
     4021 gggaattcca tactcagaaa tcagttcacc aagccagatg ccaagcatgg taccccccat
     4081 catgctgatg atggttgtca gcgttggaaa aatattctct cccttcaatc caaaatcata
     4141 ggtgaacaat ggggatgtgc ccgccagctg acggaagatc tgtatctgcc caaatgcact
     4201 gagtgccgat agcgggatcg taagaaggat ggtatatttt tcagttagct tgcgaccctc
     4261 ttttggatta tcctggatcc ttttctgcag tgaaggaatc aacggaacaa gcaattgaat
     4321 gatgatctgg gcggtaatgt acggatatac acccattgcc agaatggaaa agttcgataa
     4381 cgttccaccg gaa
//
LOCUS       DAOSDS010000153         4267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_7299_length_4267_cov_5.500237, whole genome shotgun sequence.
ACCESSION   DAOSDS010000153 DAOSDS010000000
VERSION     DAOSDS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7299_length_4267_cov_5.500237"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..1520)
                     /locus_tag="PLL88_11395"
     CDS             complement(213..1520)
                     /locus_tag="PLL88_11395"
                     /inference="COORDINATES: protein motif:HMM:NF015568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HOJ02209.1"
                     /translation="MFNTIFNGFKSFMAQPALLLGLVAAIGLIALKKPFTRVLSGFVK
                     TAVGVLILNVGSGSITNTFRPIIVGMKDVLGIEGVIIDPYTTLPAVQAAAAEQNPDLL
                     SWVGYVMVLAFLINILLVALRKITKLRALFLTGHIMFLHSTLMTWLVWYFFQLPMWPT
                     VFISATLVAVYWTVGSSILIKPTDIITNGAGFTVSHQQHLMNWVSWKIAHIFGDPDKE
                     DIDKLKLPGWLNVFSDNVVAVPLIMVIFFTPILLWLGPAALGFEKMNFDYFIQVLLLD
                     LQFGVYVTIILTGVRMFVAELAVSFNGISEKLLPGAVVGVDGMAIAPYSPNAVIAGFI
                     CCAIGEAIGVVTLIATKSPILIIPGFVPVFFDAAVTAVFANKFGGWKAVVIISIVVGL
                     IQVFGSAWAASMSGLVGGWQGNSDFATVWPAMMTIFKAISPIR"
     gene            complement(1627..2592)
                     /locus_tag="PLL88_11400"
     CDS             complement(1627..2592)
                     /locus_tag="PLL88_11400"
                     /inference="COORDINATES: protein motif:HMM:NF014212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02210.1"
                     /translation="MAFVRTILGDIDPKEMGVTNTHEHLIRIGGAEVALHGEDYRLPD
                     VDKAVQEAERFMKAGGKTIVDCNPIGVGRDIVKLLEVNEKLPGLHLIVTTGFHASHVY
                     DKVVHWVVRYSVEQIADLIIAEIEEGIDEHDYMGPIVKRSKAKAGCIKIGTSYGMITP
                     FEEKEIKAAAIAAKETGAVINTHTSHGTMALEQVQWFLKLGVKPEQIMVGHVQRIPDA
                     WYHKRIAALGASFSYDGGYRVKYWPDRFRVELIKEMIKAGYQKHITLSTDSGKRSYQI
                     AYGAGTGVDYDLVEFVPRLREEGVSEDAIEDILVNNPARLFTFKK"
     gene            2814..3500
                     /locus_tag="PLL88_11405"
     CDS             2814..3500
                     /locus_tag="PLL88_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ02211.1"
                     /translation="MTHSPKNFGIIWDMDGVLVNTAEAHYSAWKEILSKYGVDFSRKH
                     FDDTFGINDRALIMEVLQPIPPDETIQEIANQKEILYRKSIEKSLVVYPGAKEILKAC
                     RESGFKQALDSSAPQENIDAAMRISRLAQYFDAIISTDHLPSKEFPDAYLAASIALNI
                     PPDHCLVIEDSILGITGAKKAGMACIGIASTHPADRLQKADLVISSIEELDLNKIRNS
                     LGIQEGDHYV"
     gene            3493..>4267
                     /locus_tag="PLL88_11410"
     CDS             3493..>4267
                     /locus_tag="PLL88_11410"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOJ02212.1"
                     /translation="MSREYNDLLFQVARLYYEQQKTQAEIGHIIHSSRPTVSRLLKEA
                     RKRGIVDIKLNYPFEREYGLEEEFQKEFNLKEVRVLKSHNQNTDETIKGLGFFAAEYL
                     DSLIEDGDIISCSYGRTVASTIQALHPDRKLNITVVQMIGALGVENPFIDGPDLVRLL
                     SDKYGGKYVYLLAPLVVSDKNAREALVKQFRYQEAIKLSQSARIGLSGIGGMNEESPS
                     PIWTNYLNHREWADLRNHGAVGHMGAYFYDENGEILDADI"
BASE COUNT         1205 a         1032 c          951 g         1079 t
ORIGIN      
        1 ctagtcgtaa aactacactt gaaccctgtc cgttaccaca aactgctaga atctttaaca
       61 tcgatttctg tatccttctt tcaatttttt tgctcctgga taagagaatt tttacccagg
      121 agcatttttg ttggtagttt tgttaataca actgtttttc tctacgaata aacttctcta
      181 ttgtatacca ttaatcattg cgtattaaca gactaccgga tcggggaaat cgccttgaag
      241 atggtcatca ttgccggcca gactgttgcg aagtcggagt tgccctgcca tccgccaacc
      301 aaacctgaca tgctggcagc ccaggcgcta ccgaatacct ggatcaaacc gacaacgatc
      361 gagataatga ccaccgcttt ccaaccgccg aacttgttgg caaataccgc agtgacggct
      421 gcatcaaaga agaccggtac aaaaccaggg atgatgagga tgggactctt ggtggcaatc
      481 aaggtaacga caccaattgc ttcgccaata gcacagcaga tgaaaccggc aataacagcg
      541 tttggagagt agggagcgat tgccattcca tcgacaccaa ccacggcacc ggggagcagt
      601 ttctcggaaa taccattgaa ggaaacagcc agttcggcaa cgaacattct tacaccagtg
      661 aggataatgg taacgtacac accgaattgg aggtcaagaa gcagaacctg gatgaaataa
      721 tcgaagttca ttttttcaaa gccaagtgct gcgggaccaa gccacagcag aatcggggta
      781 aagaaaatga ccatgatcaa cggaactgcc accacattat ccgagaaaac attgagccaa
      841 ccgggtagtt ttagcttgtc aatatcctct ttatccggat cgccaaaaat atgggcgatc
      901 ttccatgaca cccagttcat caagtgctgc tggtgggaaa ccgtgaagcc agcgccattg
      961 gtaatgatat cagtaggttt aatgagaatg ctggaaccaa cagtccaata cacagcaacc
     1021 agagtagctg agatgaaaac ggtcggccac atgggaagct ggaagaaata ccagaccagc
     1081 caggtcatca aggttgaatg caggaacatg atgtgcccgg tcaagaatag cgcgcgtagt
     1141 tttgtaattt tgcgcaaagc gaccaagaga atattaatca agaatgccag taccatcaca
     1201 tagccaaccc aagaaagcag atcggggttc tgctcagcag ccgcagcctg tacggcgggt
     1261 agggttgtat aaggatcgat gatcacgcct tcgattccca gaacatcctt cataccaacg
     1321 atgatggggc ggaatgtgtt ggtgattgag cctgacccta cgttcaggat caaaacgccg
     1381 actgctgtct taacaaaacc agaaagaaca cgggtaaacg gtttcttgag agcaattaaa
     1441 ccaatggctg ccactaaacc aagcagaagc gccggttgcg ccatgaatga tttgaaacca
     1501 ttaaagatcg tattgaacat gtgaattact cctttttaat tttcgtgcaa aaatacttgt
     1561 aaatctttct ttctaaaaat tttgcttaaa acaattctgt ctacctcgct ttcacctcct
     1621 ccgcctttat tttttgaacg taaataagcg tgccggatta tttacaagaa tatcttcaat
     1681 tgcatcttct gatacgcctt cttcgcgcag acgaggaacg aattctacca gatcataatc
     1741 cacgcctgtc cccgcaccat atgcgatctg atatgacctc tttcctgaat ctgttgagag
     1801 cgtgatgtgt ttttgatagc cagctttgat catctctttg atgagttcaa cgcggaagcg
     1861 atcaggccag tactttacgc ggtatccgcc gtcgtagcta aagctggcac ccaacgcagc
     1921 gattctctta tgataccagg catcgggaat cctctggacg tgccccacca taatctgttc
     1981 gggttttacg cccaatttca aaaaccattg cacctgttca agcgccattg tgccatggga
     2041 ggtgtgcgta ttgatgacag caccggtctc tttcgcggcg atggctgccg ctttaatctc
     2101 tttctcttca aatggcgtga tcatgccgta tgaagtgcca atcttgatgc agccagcttt
     2161 ggctttgctg cgtttcacaa tgggtcccat ataatcatgc tcatcaatcc cctcttcgat
     2221 ctctgcaatg atcaggtcgg cgatctgctc gaccgaatag cggacgaccc aatggaccac
     2281 cttgtcgtac acatggctgg catgaaaacc agtagtgacg ataagatgca aaccaggcag
     2341 tttttcattg acctccaata atttcacgat atcgcgaccc acaccaatag gattgcaatc
     2401 tacgatggtt ttgccaccgg ctttcatgaa gcgttcagct tcctgaaccg ctttgtcaac
     2461 atcgggcagc cgatagtctt cgccgtgcag cgccacttct gccccaccga tacggattag
     2521 atgctcgtgg gtattcgtca cacccatctc ttttggatca atatcaccaa gaattgtgcg
     2581 tacaaaagcc ataatgtatt cctctttgta ttaataattt attagaattt ttcacttcta
     2641 ttctagtttc attttgaatt taatcaacat attttcagaa taatgaacat atgtgcatca
     2701 tggctttcgc ggagattctc gtgagattgc tattacgcaa agcattgaga tagtgttatt
     2761 atattacaat atttcgtcgt gctgaacata tgtgcaaaag gaaaccaaag aagatgacac
     2821 attcaccgaa aaattttggc attatctggg acatggatgg agtactcgtc aacaccgccg
     2881 aggcgcacta ctctgcctgg aaagaaattc tttcaaagta tggcgtggac ttttcccgaa
     2941 agcactttga cgacactttt gggatcaacg atcgcgctct gatcatggaa gttcttcaac
     3001 caatccctcc tgacgaaact attcaggaga tcgcaaacca aaaagagatc ctgtatcgga
     3061 aaagcattga aaaaagtctg gtcgtctacc cgggtgcaaa agaaatcttg aaagcttgcc
     3121 gtgaaagcgg gttcaaacaa gcgctggact cctcagcccc gcaagaaaac attgacgcag
     3181 caatgcgcat cagtcgattg gcgcagtact tcgacgccat catttcaaca gatcatctcc
     3241 ccagcaaaga gtttccagac gcctacctgg ctgcctcaat cgcgctcaac attccccctg
     3301 accattgcct tgtgatcgaa gacagcattc tgggtatcac aggggcaaag aaagccggaa
     3361 tggcatgtat cggcatagcc agcacccacc ctgcagaccg tttgcagaaa gctgatctgg
     3421 tcatatcttc tattgaggag cttgatctaa acaaaattcg aaactcttta ggtattcagg
     3481 agggtgatca ctatgtctag agaatacaac gatcttcttt ttcaggttgc cagattatat
     3541 tacgaacagc aaaagaccca ggctgagatc ggacacatca tccattcttc ccgccccacg
     3601 gtctctcgcc tgcttaaaga agcccgcaag cgcgggatcg tagacatcaa gctcaattac
     3661 ccctttgaac gtgagtatgg attggaggaa gaattccaaa aggaatttaa tttaaaagaa
     3721 gtccgcgtcc tgaaatccca taaccaaaat accgatgaaa ccatcaaagg attgggattc
     3781 tttgcagcag aatacctgga ctcactcatt gaagacgggg atatcatttc ctgttcgtat
     3841 ggtcgaacgg ttgccagcac cattcaagcg cttcatcccg atcggaaatt gaatataacg
     3901 gttgtacaga tgatcggcgc attgggcgtg gagaacccct ttattgatgg tcctgacctg
     3961 gtgcgtttac tgagcgataa gtacggggga aaatatgtgt atttattggc gcccctggtg
     4021 gtgagcgata agaacgccag agaagccctg gtaaagcagt tccgttacca ggaagctatc
     4081 aaattgtcac agtctgccag gatcggcttg tctggaattg gcgggatgaa cgaagaatct
     4141 cccagcccca tttggaccaa ttacctcaat catcgcgaat gggcggatct gcgaaaccac
     4201 ggtgcggttg ggcacatggg cgcgtatttc tacgatgaaa atggagaaat tctcgacgct
     4261 gacatta
//
LOCUS       DAOSDS010000154         4251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_7335_length_4251_cov_4.164919, whole genome shotgun sequence.
ACCESSION   DAOSDS010000154 DAOSDS010000000
VERSION     DAOSDS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7335_length_4251_cov_4.164919"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLL88_11415"
     CDS             complement(<1..230)
                     /locus_tag="PLL88_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HOJ02213.1"
                     /translation="MNEKMIPITDIGGIQIGQVSDQENWTGCTVILCKEGAVGGVDQR
                     GGAPGTRETDLLRPMHLVEKVHAILLAGGSAFG"
     gene            331..1287
                     /gene="gnd"
                     /locus_tag="PLL88_11420"
     CDS             331..1287
                     /gene="gnd"
                     /locus_tag="PLL88_11420"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HOJ02214.1"
                     /translation="MYNKEKSADQSKVAEMKIALIGLGKMGSNMARRLKKGGIDVVGY
                     SLSKEDAQRLADEIGMEVAFAYKEIVEKLTPPRIIWSMVPAGEATKEVAQTFYALLEQ
                     GDYFIDGGNSYYKDSEARAKEFRNKGIHFFDVGVSGGIWGLEEGYSLMIGGDQKAADM
                     LIPLFQALAPSQENGWGFVGPSGAGHFVKMVHNGIEYGMMEAIAEGFEVMKAKQEFGL
                     DLEKIASIWQFGSVVRSWLLDLTRDALREDAELKDIESWVEDSGEGRWTLHEAIDLAV
                     PTPVISASLNRRFDSRQTDSFALKLLSAMRHKFGGHAIKGNK"
     gene            1297..2772
                     /gene="zwf"
                     /locus_tag="PLL88_11425"
     CDS             1297..2772
                     /gene="zwf"
                     /locus_tag="PLL88_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOJ02215.1"
                     /translation="MDKSAEIGFIIFGVTGDLTKRKLIPALYELSQEGRIPGKLRIVG
                     FARRPWTNDEMREILAEGIDEFARVKPVNRQIVEELLAHAEYVQGEFEKPDGFRRLIE
                     RLEESKIKNRIFYLATPPSAYPLIIDGLRQCERATGSGWTRIVIEKPFGSDRVSARLL
                     DEKLHSCFQENQIYRIDHYLGKETVQNILVFRFANGIFEPLWNNHYVDSVQIMVAETV
                     GVESRAGYFDGMGIIRDVFANHMLQMLSLTAMESPYAFNAHSVRDEKMKVLKALRPPE
                     GQEVLAVTYRAQYGHGTINGQEIKGYLEEERVEPHSQTETYFAMKLFIDNWRWAGVPF
                     YLRCGKRLKKRATEIAIQFKQVPLSLFNWRNLAGDAPNILRLRLQPDEGINLSFGAKV
                     PGPINEITPVDMDFCYSDAFGSEPPEAYERLLLDCMMGDATLFTRSDEALAQWDFTTG
                     ILKAWDEHPEEKLPIYQPGSWGAPGADELINKDGRSWLNPK"
     gene            complement(2773..3357)
                     /locus_tag="PLL88_11430"
     CDS             complement(2773..3357)
                     /locus_tag="PLL88_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOJ02216.1"
                     /translation="MEHKIFHGQFSPEELAECLQIHFNRGNLVVQRIEYEDGLGVQIK
                     TREFRTSGGDTALGIFLKKVEDGVSVQVGQQNWVGIAASIGYSALATFINPVNLLGRL
                     DDIAQDVEYLQLTDEVWKVLESNARALGSGYELSEKLRRIACEYCGAANQAGSATCVA
                     CGAPMGAVQPFSCKYCGFILLEKTAICPNCKRNL"
     gene            3395..4015
                     /locus_tag="PLL88_11435"
     CDS             3395..4015
                     /locus_tag="PLL88_11435"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOJ02217.1"
                     /translation="MMIKRILLILPVVIAVVLVFLVNNTVNADFRFQQPTISIPTVTG
                     TPQGAMVYVDLFQEDPVNVRSGPGVFYDEIGILLPGESAPALGRSAGGDWILISYVGV
                     ESGTGWVYSPLVTLSGGELSIVEPPPSPTPETTATIDATLAAQFLTTPAATNLPTYTA
                     APPQTMPNYVDEVSSGISQHIPIGLIILIIGGLGVLIAVISFITER"
     gene            complement(4012..>4251)
                     /locus_tag="PLL88_11440"
     CDS             complement(4012..>4251)
                     /locus_tag="PLL88_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02218.1"
                     /translation="MQTVMANMQLQLTLPDVELRPDTSKYFFIDKVDPFDLIQKGKEI
                     VYTSLAQIEAGIQSFQRSDHVPSGLFLSELTVDQG"
BASE COUNT         1139 a          906 c         1108 g         1098 t
ORIGIN      
        1 ccaaaagctg agccacccgc caatagaata gcgtgcactt tttctaccag gtgcattgga
       61 cgcagcagat ctgtttctcg ggtgccggga gcgccacccc gttgatcaac accgccaact
      121 gctccttctt tgcatagaat gacggtgcac ccggtccaat tttcctggtc actgacttgt
      181 ccgatttgga tgccgccgat atcggtgatt ggtatcattt tttcgttcaa tcctgcctcc
      241 cgatgtcgat agaagattat ctatattcta atataggaaa ataagatgat ttcgatatgc
      301 ggatagatga aaaattgaga tacaaaagga ttgtacaata aagaaaagag tgcagatcaa
      361 tcaaaggtgg cagagatgaa aatagctttg atcgggctgg ggaagatggg atccaacatg
      421 gcacgcagat tgaagaaggg ggggattgat gtggtgggat acagcctttc gaaagaagat
      481 gcccagcgtc ttgctgatga aattggtatg gaagtggcgt ttgcatataa agagatcgtg
      541 gaaaaactga ccccaccgcg catcatttgg tccatggtgc cggcggggga ggcaacgaag
      601 gaagtggcac aaacatttta tgctcttctt gaacagggtg attatttcat tgatggcggg
      661 aactcttatt acaaagattc tgaggcacga gcaaaggagt tcaggaataa aggaatccat
      721 tttttcgatg taggggtaag cggcgggatc tggggattgg aagaaggata ttcattgatg
      781 atcggagggg atcagaaagc ggctgacatg ctgataccat tgtttcaagc attggcgccc
      841 tcccaggaaa acggatgggg gtttgttggt ccaagtggcg cagggcattt cgtgaaaatg
      901 gtccataatg gaatcgaata tgggatgatg gaagccattg ctgagggctt tgaggtcatg
      961 aaagcaaaac aagagttcgg tcttgacctg gaaaagatcg cgagcatatg gcaattcggt
     1021 tctgtggtgc gttcctggct gctggattta acccgcgatg ccttgcggga agatgcggaa
     1081 ctgaaggata ttgagagttg ggtggaagat tcgggcgaag ggcgttggac tttgcatgaa
     1141 gcgatcgacc tggcagttcc gacaccggtt atttctgcgt cgttaaatcg acgttttgat
     1201 agcagacaga cggatagttt cgcgctcaaa ttgctctcag ccatgcgcca caagttcggc
     1261 ggacatgcga taaaaggaaa caaatgatgg tgaaaaatgg ataaatcagc cgaaattggg
     1321 ttcatcattt ttggagtaac gggagatctc acaaaaagga aattgatacc ggctctctat
     1381 gaactttccc aagagggtcg tatcccgggg aaattacgca ttgtggggtt cgccaggcga
     1441 ccttggacaa atgatgaaat gagggaaatc ctggcagaag ggattgatga gtttgccagg
     1501 gtcaaacctg tgaacaggca gatcgttgaa gagttattgg cgcatgcaga gtacgttcaa
     1561 ggagaatttg aaaaaccaga cgggttcagg cgattgatcg aaagactcga ggaaagcaag
     1621 atcaagaatc gcattttcta tttggcaacg ccgccttctg cctatccatt gatcatcgac
     1681 ggtctgagac aatgcgagag agcaacggga tctggatgga cgcgaattgt cattgagaaa
     1741 ccctttggga gcgaccgtgt atctgccaga cttctggatg agaaattgca ttcctgtttt
     1801 caggaaaatc agatttatcg gattgatcat tatctaggca aggaaactgt acagaacatt
     1861 cttgtctttc gatttgctaa tggtatcttc gagccactgt ggaacaatca ttatgttgat
     1921 agtgtgcaga tcatggtagc tgaaactgtg ggggttgaat cacgtgcagg gtattttgat
     1981 ggcatgggca tcattcgtga cgtgtttgcc aaccatatgc ttcaaatgct ttcgctgaca
     2041 gctatggagt caccgtatgc attcaacgct cattccgttc gggatgaaaa aatgaaggtg
     2101 ctcaaagcgc tcagaccacc agaaggacag gaagtgctcg ccgttaccta ccgagcgcaa
     2161 tacggtcatg gaacaatcaa tggtcaggag atcaaagggt atttagaaga agaacgggtt
     2221 gaaccgcatt cccaaacaga aacatatttt gccatgaaac ttttcatcga taattggcgc
     2281 tgggcggggg tgccattcta tcttcgctgc gggaaacgct tgaaaaaaag agcgacggaa
     2341 attgctatac aattcaaaca ggtcccgctc tccctgttca attggcgaaa tcttgcgggg
     2401 gatgctccca acattctgag gcttaggctg cagccggatg agggaataaa cctttctttt
     2461 ggtgccaagg ttcccggtcc gatcaatgag ataacccctg tcgatatgga tttttgttac
     2521 tctgatgcct ttggcagtga accacccgaa gcatatgaaa gattgctgct cgattgtatg
     2581 atgggagatg cgacgctttt taccagatcg gatgaagccc tggctcaatg ggatttcacc
     2641 acagggatct taaaggcgtg ggatgagcac cccgaagaaa agttgccgat ataccagcca
     2701 ggaagttggg gtgcgccggg tgctgatgaa ctcatcaaca aggatgggcg tagctggctg
     2761 aatcccaaat agttaaaggt tccttttgca atttggacag atcgcggttt tctctagcag
     2821 gataaagcca cagtatttgc acgagaaggg ctggacggca cccatgggag ctccgcatgc
     2881 aacgcaggta gcacttcctg cttgatttgc tgctccgcaa tactcacagg cgatcctgcg
     2941 caatttttct gataactcat atccgcttcc aagtgcgcgg gcattgctct caagaacttt
     3001 ccatacttca tcggtaagct gaaggtattc aacgtcctgg gcaatgtcat ccaatctgcc
     3061 taagaggttg accggattga tgaaggtagc cagggcggaa tagccgatgc tggcagcaat
     3121 tcccacccaa ttctgctgac caacctgcac cgaaacacca tcttcaactt ttttgagaaa
     3181 aattcccaaa gcggtgtcgc cgccggaagt gcggaattct ctggttttaa tttgaacacc
     3241 aagaccatct tcatattcga ttctctgaac gaccagattg ccacggttga agtggatctg
     3301 aagacattct gcaagttctt ctggcgagaa ttgaccgtga aatatcttgt gttccattgg
     3361 attgcattat aatcaaaaca ctgaaaatga gttaatgatg ataaaacgaa ttttattaat
     3421 tcttccggtt gtgattgctg tggtattggt ttttctcgtt aataatacgg taaatgccga
     3481 ttttcgtttt caacaaccca cgatttccat tccaactgtc accggcacac cgcagggagc
     3541 catggtgtat gtggatctgt tccaggaaga tccggtcaat gtgcgttcgg gtccgggcgt
     3601 tttctatgat gaaatcggta tcttgcttcc tggtgaatca gcacctgctt tggggcgatc
     3661 tgctggcggc gattggatat taatatccta tgttggagtt gaatcaggaa ccgggtgggt
     3721 gtattcaccg ttggttacat tatcaggtgg tgaattatcg atcgttgaac cgccaccaag
     3781 ccccacaccc gaaaccaccg ccacaattga cgcaacgctc gcagcgcaat tccttactac
     3841 acctgcagcc accaatctgc caacctatac cgcagcgcct ccgcaaacaa tgcccaatta
     3901 tgtggatgag gtatcatctg gcatatccca gcacatccca atcgggctga tcattctgat
     3961 cattgggggc ttaggcgtgt tgatcgctgt gatatcgttc attacagaac gctaaccttg
     4021 atcaacggtt aattcagaaa gaaataaccc tgagggcaca tgatcagatc gctggaatga
     4081 ttggatgcca gcttcaatct gtgccagcga tgtgtagaca atctctttcc ctttctggat
     4141 caaatcaaat ggatctacct tatcgataaa gaaatattta ctggtatcgg gtcgcaattc
     4201 cacgtctggt aacgtcaatt gaagctgcat gtttgccatg actgtttgca t
//
LOCUS       DAOSDS010000155         4203 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_7439_length_4203_cov_5.668033, whole genome shotgun sequence.
ACCESSION   DAOSDS010000155 DAOSDS010000000
VERSION     DAOSDS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7439_length_4203_cov_5.668033"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="PLL88_11445"
     CDS             complement(<1..851)
                     /locus_tag="PLL88_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOJ02219.1"
                     /translation="MRTIKDTWDDWIKDATIVRVIRNSGYLLFSNIFSVVQSVLTGRL
                     LGIMGFGIVGTITTFASTLNRLFSFRMNELVVKYYGEAMEKKQPERAAAVIKASLLAE
                     SITSIISFGFCAFFASYAAANIADDPTLTPHFILYGTIILANVFMETSTGVLQVNGKF
                     RLEAFITFLGSVCTAAIIVWAYLAKGGLLEIVVAYMAGKFVLGIGTALLGFKELRKTL
                     GKNWLRTPFSYLPPIKELTKFAFSTNISSTIMMLVRDNEVLWVAYFLSPLEAGYARAA
                     LAIINFV"
     gene            complement(848..1825)
                     /locus_tag="PLL88_11450"
     CDS             complement(848..1825)
                     /locus_tag="PLL88_11450"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ02220.1"
                     /translation="MATYVVTGAAGFIASKVCEFLLEADNSVIGIDNFDPMYDLRLKD
                     WRLKRLQAFPKFTFHREDICNLTILRDIFSTSLQVDAVINLAAKAGVRDSVLDPWSYY
                     NTNVTGTLNLLEICKERKIKKFILASTSSIYGNDAPYPTLETSDSSHPLQPYAASKKA
                     AETLSYSYHYLHGLDVSVVRYFTVYGPAGRPAMSMYRFTKWIAEGETVTIYGDGNQTR
                     GFTYVDDIARGTIQALKPLGYGIFNLGGHQTISINDLIHTFESLLGKKAVLQYLPSNP
                     ADMNASWADVSKAKEVLGWVPEVPLEKGIPHIIDWYMQERSWASQVSTE"
     gene            complement(1825..3141)
                     /locus_tag="PLL88_11455"
     CDS             complement(1825..3141)
                     /locus_tag="PLL88_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOJ02221.1"
                     /translation="MNYAKDLITKFNNKTARVGVLGLGYVGLPLAIVFAQAGFDVTGI
                     DPMKEKVNSLQQGKSYVLDVPDETVKILTGSGKLKATSDFSVLRELDAVSICVPTPLR
                     KTGDPDLSFILSVADSLKQYVHAGMAVVLESTTYPGTTREMILTKLTEGNDLKIGENL
                     FLAFSPERVDPGRVDFTTTNTPKVIGGITPTCLEVTACWYGQAIQKVVPVSSAEVAEM
                     AKLLENTFRMINIGLVNEMAMMCDRLGVDIWEVIDAAATKPFGFMKFQPGPGLGGHCI
                     PIDPLYLSWKLKTLNYSTRFINLASEINTGMPRYTVAKVQDTLNLHKKSINGSKILVL
                     GVSYKPDIDDLRESPALDIIHLLQEKGAQVQYHDPFIPTIDHDELRLQSVADLDKAVK
                     DADCVVIATNHSLYDYSKILLDAKLVFDARNATGELGKNNPKVVKL"
     gene            complement(3151..3795)
                     /locus_tag="PLL88_11460"
     CDS             complement(3151..3795)
                     /locus_tag="PLL88_11460"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ02222.1"
                     /translation="MTSQDTLVDIRAEIAQCTKCPLARTRKNTVPGEGSADAEIMFIG
                     EGPGFYENEQGKPFVGQAGKFLDELLAYAGLKREQVFITNVVKCRPPDNRDPEPSELE
                     ACSSFLDRQIACLHPKVIVTLGRHSMGKFLPNAKVSQIHGRSAWVRGQLIVAMYHPAA
                     GLHQPTLKSTIIADFRKLPEIIEQARKSEMIQKANPQSEEEEPHKEDPTQLSFF"
     gene            complement(3846..>4203)
                     /locus_tag="PLL88_11465"
     CDS             complement(3846..>4203)
                     /locus_tag="PLL88_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02223.1"
                     /translation="DIPLICVLQAGRDKLAACRYGYQDRWVAQGDPFILSPQELSEEI
                     NEETIICGEMDAEERRVISRKWKKVHLCSTANCVRRAAFLAELAAQRCAQGKFDNPMS
                     LAPIYLHTANPILAEQ"
BASE COUNT          954 a         1066 c         1106 g         1077 t
ORIGIN      
        1 tggacaaaat tgatgattgc cagcgcagca cgggcgtaac cggcttcgag cggcgagagg
       61 aaatacgcca cccataatac ttcattatcg cgcaccagca tcatgatggt gctgctgatg
      121 ttggtgctga aagcgaattt ggtcaattcc ttgatgggcg gcagatacga gaagggtgtt
      181 ctgagccagt tcttgcccag tgttttgcgc aattctttga aaccgagcag agcggtgccg
      241 atacccagga cgaattttcc agccatatag gcaaccacga tctccagcaa gccacccttc
      301 gccagataag cccacacgat gatggcggct gtacaaacgc tgccgagaaa cgtgatgaag
      361 gcttctaggc ggaacttgcc gttgacctgc agtaccccgg ttgaggtttc catgaagacg
      421 tttgccagga tgatcgttcc atacaggatg aaatgcggcg tgagggtggg gtcatctgca
      481 atattggcgg cagcgtagga tgcgaaaaag gcgcaaaacc caaaagatat tattgaggtg
      541 atcgattcgg cgagtagaga tgccttgatc acagcggctg cccgctcggg ttgttttttt
      601 tccatcgctt ctccgtaata tttcaccacc agttcattca tgcgaaatga aaacaagcgg
      661 tttagggtgg aagcgaatgt tgtgatagtg ccgacaatgc cgaagcccat gatgcccaac
      721 aggcgaccgg tcaacacact ttgaacgacg ctgaaaatat tactgaagag caggtatccg
      781 gaattgcgaa ttaccctcac gatggttgca tctttgatcc aatcatccca ggtgtctttg
      841 atggttctca ttctgttgaa acctggctag cccaggagcg ctcctgcatg taccaatcga
      901 tgatgtgtgg gatgcctttt tccaagggca cttcgggaac ccaccccagc acctctttgg
      961 ctttgctcac atctgcccag ctcgcgttca tatctgccgg gttagacggc agatattgca
     1021 ggacggcttt tttccccaac aagctctcaa aagtgtggat caagtcgttg atcgaaatgg
     1081 tctggtgccc gcccaggttg aaaatcccgt atcccagggg ctttaaagcc tggatggttc
     1141 cgcgtgcgat atcatccaca taggtgaatc cccgcgtctg gttgccatcg ccataaatgg
     1201 tgactgtttc cccctctgca atccactttg tgaagcggta catcgacata gccgggcgac
     1261 cggcgggtcc gtacacggtg aaatagcgca ccacgctgac atccaacccg tgcaggtaat
     1321 gatagctgta gctcagggtt tccgcagctt ttttgctggc ggcatatggt tgcagaggat
     1381 ggctggaatc gcttgtttcg agggtcggat agggggcgtc gttgccgtag atgctggaag
     1441 tggaagccag gatgaatttc ttgatcttgc gctccttgca gatctccaat agattcagcg
     1501 tgccggtcac attggtattg taataagacc agggatcgag cacggaatcg cgcacaccgg
     1561 ctttggcagc caggttgatg acagcatcta cctgcagact cgtcgaaaag atgtcccgca
     1621 atatggtcag gttgcagatg tcttcgcgat gaaatgtgaa cttcgggaat gcctgcaggc
     1681 gtttcaagcg ccagtctttc aagcgcaggt catacatcgg gtcaaagttg tctatcccga
     1741 tgaccgagtt gtcagcttcc aacaggaatt cgcagacttt ggaggcgatg aagcctgccg
     1801 cgccggttac aacataagta gccattacaa tttcaccacc ttaggattat ttttgccgag
     1861 ttcgccggtc gcgttgcgcg catcgaagac cagtttggca tccagcagga tcttggaata
     1921 gtcgtacagg ctgtggttgg tcgcaatcac cacgcaatcg gcatctttca ccgccttgtc
     1981 aaggtcggca acggattgca ggcgcagttc gtcatggtca atagttggga tgaatggatc
     2041 gtgatactgc acctgtgcgc ctttctcttg cagcaggtga ataatatcca gtgcaggcga
     2101 ttcgcgcagg tcatcgatat ctggtttata cgatactccc aataccagga ttttgctgcc
     2161 gttgatggac tttttgtgca ggttcaacgt atcctgcact ttggcaaccg tgtagcgcgg
     2221 catgccggtg ttgatctcgg aggcgagatt gatgaagcgc gttgaataat tcagcgtttt
     2281 caatttccag gaaagataca gcgggtcaat gggaatgcag tgcccaccca gacccggacc
     2341 cggttgaaac ttcataaaac cgaaaggctt ggtggctgca gcatcgatca cttcccaaat
     2401 atctactccc aaccgatcgc acatcattgc catctcgttg accaaaccga tgttgatcat
     2461 gcggaaggta ttttcgagca atttcgccat ttcagccact tcggcggaag agaccggaac
     2521 gactttctga atggcttgcc cataccagca tgcggttacc tcaaggcatg tcggggtgat
     2581 tccgccgatc acttttggcg tgttggtggt cgtgaaatcc acccttccag ggtcaacccg
     2641 ctcgggagag aacgccagga atagattctc tccgatctta agatcgttgc cttcggtcag
     2701 cttggtgagg atcatctcgc gcgtggtacc ggggtaggtc gtcgattcga gcacgaccgc
     2761 catgcccgcg tgcacatatt gtttcaagga atccgctacg gaaaggatga aagagaggtc
     2821 gggatcgccg gtcttgcgca atggcgtggg gacacaaatg ctgaccgcgt ctaattcccg
     2881 caggacggaa aaatcgctgg tggcttttaa ttttccgctg ccggtcagga tcttgacggt
     2941 ctcatcaggc acgtccaaca cgtagctttt tccttgctgt agactgttca ccttttcttt
     3001 catgggatca atcccagtca cgtcaaaacc ggcttgtgca aaaacgattg ccagcggcag
     3061 acccacatac cccaagccca gtacgccgac gcgagcggtc ttgttgttga atttcgtgat
     3121 caggtctttt gcgtaattca tccttttctc ctagaagaaa ctcaattggg ttgggtcttc
     3181 tttatgaggt tcttcttctt cactctgcgg gtttgccttt tggatcattt cggacttgcg
     3241 agcctgttcg atgatctcag gcagtttgcg gaaatctgct atgatggtcg atttgagagt
     3301 gggctggtgc agcccggcgg ctggatgata catcgctaca atcaattgac cgcgcaccca
     3361 ggcagatcgc ccatggatct ggctgacctt ggcattggga aggaattttc ccatggaatg
     3421 cctgccaagg gtgacgatga ctttcggatg caaacaggca atttgccggt ccagaaaact
     3481 ggagcacgct tccaattcag atggttctgg gtcacgattg tcaggagggc ggcatttcac
     3541 cacattggta atgaaaacct gctctctttt caatccagcg taagccagaa gctcgtctag
     3601 aaatttccct gcctgcccaa caaaaggttt gccttgctcg ttttcgtaaa aaccaggacc
     3661 ctcaccgatg aacatgattt cagcatcggc tgatccttct ccaggaacgg tgttctttcg
     3721 ggttctcgcg agagggcatt ttgtgcattg agcgatctcg gctcgtatat caaccaaggt
     3781 atcttgtgag gtcatgcttc atcctttctg tctgcaggta attttacaat gttctgcagt
     3841 tcttgttatt gctcagcgag aatcggattg gcagtatgca gataaatggg cgccaggctc
     3901 atgggattgt caaattttcc ttgcgcacac ctttgcgcgg cgagctccgc caaaaaagca
     3961 gcccggcgca cacaatttgc tgttgaacat aagtggacct ttttccattt tctgctgatc
     4021 accctgcgct cttccgcgtc catttctccg cagatgatgg tctcttcatt gatctcttcg
     4081 ctcaattctt gtggagaaag gatgaaagga tcaccctggg cgacccatcg atcctgataa
     4141 ccatagcggc aggctgccag cttatcacgc cccgcttgca atacacagat caggggaatg
     4201 tct
//
LOCUS       DAOSDS010000156         4129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_7624_length_4129_cov_5.171821, whole genome shotgun sequence.
ACCESSION   DAOSDS010000156 DAOSDS010000000
VERSION     DAOSDS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7624_length_4129_cov_5.171821"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PLL88_11470"
     CDS             complement(<1..410)
                     /locus_tag="PLL88_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOJ02224.1"
                     /translation="MHILVFSDIHSNLTALEKVLSVAGSYDAVWCLGDVVGYGPDPNE
                     CIALIQTLPNLICIRGNHDAAVVGDVDQHTFNHEASLAISWTKRELDADGQEFLLKLP
                     ETVIQENVTLVHGSPRNPVWDYVMDSMTALRMFQ"
     gene            complement(426..1643)
                     /gene="coaBC"
                     /locus_tag="PLL88_11475"
     CDS             complement(426..1643)
                     /gene="coaBC"
                     /locus_tag="PLL88_11475"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOJ02225.1"
                     /translation="MENPIKHKNILLGVTGSIAAYKAVDLASQLTKAGGNVDVILTPS
                     AEKLVSPLTFRSVTGKKVFTDADLWDTTSHVAHINLALPADILLVAPATANTIAKLAH
                     GQADNLLTLTALAIRCPLVVAPAMDGGMYGNAFTQENLEILRSHKITLLGLDEGTLAS
                     GLSGNGRMLEPRELAGWVRFLLSRENPLAGKRVIVTAGGTQEPIDPVRVLTNHSSGKQ
                     GYALAQAVLDAGADVTLISAPTAFTAPQGAKWVPIKTAEEMHSAVKDELQKADAVIMA
                     AAVADFRPATSQKEKIKKEGKLQEIQLEETVDILKELGEFKRNQKPELILIGFAAESE
                     NLLQNAAKKLAQKNLDLIVANDISRENAGFAVDTNAVTLLFKDGKKRDLPLQDKELVA
                     QEIIDILINIMQS"
     gene            complement(1627..2409)
                     /locus_tag="PLL88_11480"
     CDS             complement(1627..2409)
                     /locus_tag="PLL88_11480"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOJ02226.1"
                     /translation="MLLIIDLGNTNLALGLYEGQQLASHWRLATDHARMPDEYGMQFR
                     DLLDHHHIPVDTIEGICLSSVVPPLTERVVEACQVYLNKKPLVATSDLKLNILIKYED
                     PKAVGADRIADAVEVQRTYGGPACIIDFGTATTFNALTESGDYLGGSILPGIQVSADA
                     LVQRTAKLPAVALEAPPSVIGKNTADAIRAGLIYGYVSLVEGMVARYRKILGEQMKVI
                     GTGGLIQLISPHTNAITIIEPWLTLNGLQHIWEMNKDHGKPH"
     gene            complement(2493..>4129)
                     /locus_tag="PLL88_11485"
     CDS             complement(2493..>4129)
                     /locus_tag="PLL88_11485"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HOJ02227.1"
                     /translation="EELVDEINQTGVELARRIVRASYKDVLIAGDVGPLGVRLAPFGR
                     VQAEQAHEVFKEQIQALVEAGCDLIVIETVTDLYEVREAISAAREVAIDMPVVASMTF
                     TRDDRTLLGDDPQKVATKIFSYGADVIGINCSGGPNQLLRLLKKMKKVVPDGLFWVKP
                     NAGWPEQISGRIFYPASAEYFGEYALAFWQAGAGVMGGCCGTTPAHIASIRQAIDNHA
                     TPATLNGTTLELVEEETVETETSEPSEFAQKLSAQEFTFSVEMQPPRGLATKKIVAGA
                     SMLMDSGADAINVADSPMARMRMSPWAVCSLLQNKVNVETILHFPTRGRNLLRVQGDL
                     LAAHALGVRNVFVIMGDPTAIGDYPEAMDNFDLAPSGLVKLIKQRFNQGIDQSGSDIG
                     QPTSFFVGCALNLAASDPKREMEILRRKIRNGADFVITQPVYEAALITNFISKYQTDF
                     EELTVPLLVGIMPLVSARHAAFLNQEVPGITISDAIHERMKSAEANGRGPQEGIRIAI
                     DLIKEIKNRAQGVYITPSFGRFDYVAEIIEKSKQPT"
BASE COUNT          817 a         1117 c         1080 g         1115 t
ORIGIN      
        1 aattggaaca tacgcagggc ggtcatggaa tccattacgt aatcccatac cgggttgcgt
       61 gggcttccat gtaccagggt gacgttttcc tgtatcaccg tttcaggcag cttcaacaga
      121 aattcctgcc cgtcagcgtc cagttctcgc tttgtccaac tgatggctaa actggcttca
      181 tgattgaatg tgtgttgatc aacatctccc actactgccg catcgtgatt ccctcggata
      241 cagatcagat tggggagggt ttggatcaac gcgatgcatt cgttgggatc gggaccgtag
      301 cctacaacat cccccaaaca ccacacggca tcgtacgaac cggcaaccga cagcactttt
      361 tccagcgctg tgagattgga atgaatatct gaaaagacaa ggatgtgcat gggtttttcc
      421 ttttcttaac tttgcatgat attaatgaga atgtcaatga tctcttgggc gaccaattct
      481 ttatcctgca ggggcagatc tcgttttttg ccatctttga agagcaacgt gaccgcgttg
      541 gtatccacgg caaatcctgc attttctctg gaaatatcat tggcgacgat cagatcaagg
      601 tttttttgtg ccagcttctt tgcagcgttc tgcagcaggt tttcactttc tgcggcaaat
      661 ccgatcaaga tcaattccgg tttttgattg cgtttgaact ctcccagttc tttgagaata
      721 tcaacggtct cttctaattg tatctcttgc agtttccctt cttttttgat cttctctttc
      781 tggctcgttg ctgggcgaaa atcagccact gctgcagcca tgatgacggc atctgctttc
      841 tgaagctcat ctttcacagc cgaatgcatt tcctctgctg tttttatcgg aacccatttt
      901 gcaccctggg gggcagtgaa cgctgtgggc gcggaaatga gcgtgacatc tgcgccggcg
      961 tccagaaccg cttgtgccag cgcatagccc tgcttgcctg aagaatggtt cgtcaatacc
     1021 cgcaccgggt caatcggttc ttgcgtgccc ccggcggtga cgatcaccct ctttcctgcc
     1081 agagggtttt cccggcttag cagaaaacgc acccagccag ccagctctct tggttccagc
     1141 atgcgcccat ttccgcttaa cccagatgcc agggtacctt catctagtcc aagcagggtg
     1201 atcttatggg aacgcagtat ttcgagattt tcctgcgtga atgcattgcc gtacattccc
     1261 ccgtccatgg ctggtgcaac caccagcggg cagcggatag ccagcgccgt cagggtcagc
     1321 aggttgtcgg cttgaccatg tgctagtttg gcgatggtgt tggctgtggc aggtgccacc
     1381 agcaagatat ctgcaggcag cgccaggttg atgtgagcga catggctggt ggtgtcccat
     1441 aagtcagcat cggtgaacac tttctttccc gtaaccgaac gaaatgtcag gggagatacc
     1501 agtttctcgg ctgatggggt gaggatgaca tccacatttc cgccggcttt ggtgagttgc
     1561 gatgccaggt caactgcttt ataggcggcg atggagccgg tcacacccag cagaatattt
     1621 ttgtgtttaa tggggttttc catggtcctt gttcatctcc catatgtgct ggagaccgtt
     1681 tagggtcaac cagggttcaa tgatcgtgat agcgttggta tgtggactga tcagttgtat
     1741 cagtccgcct gttccaatca ctttcatttg ctcccccagg atcttgcggt aacgtgcaac
     1801 catcccttca accaggctta cgtatccata gattaagccg gctctgatgg cgtcagcggt
     1861 gttcttcccg atcaccgaag gaggggcttc cagcgctact gctggcagtt ttgccgtgcg
     1921 ttgtaccagt gcgtctgcag aaacttgaat gcccggcaag atcgaaccac ccaggtaatc
     1981 cccgctttcc gtcagcgcgt tgaacgtggt tgctgttccg aaatcgatga tgcaggctgg
     2041 tcctccatag gttcgctgga cttccacagc gtcggcaatg cggtccgcgc ccaccgcctt
     2101 ggggtcttca tactttatca ggatgtttaa cttcaagtca gatgtggcca ccagcggctt
     2161 tttgttgaga tacacctggc aggcttctac aacccgctcg gtgagcgggg ggacgaccga
     2221 ggacaggcag attccctcaa tagtgtctac ggggatgtga tgatggtcca gcaggtcgcg
     2281 aaattgcatt ccgtattcat cgggcatgcg cgcgtggtcg gttgccaggc gccaatggga
     2341 agctaattgc tgaccttcat ataatccgag cgccagattc gtgtttccca ggtcgatgat
     2401 tagcagcatg gtttcctttc ttgtctatat tctatattct aacgtaagaa aacaacccta
     2461 tgagccgtgt tctggtgtat gccgacgctt tctcatgtgg gttgttttga tttctcgatg
     2521 atctctgcca cgtaatcgaa ccgcccaaaa gatggggtga tatacactcc ctgggctctg
     2581 ttcttgatct ctttaatgag atcgatggcg atgcggatgc cctcctgtgg tcctctgccg
     2641 ttcgcttcag cgcttttcat gcgttcgtgg atggcatctg aaatggtgat gcccggaact
     2701 tcctggttca ggaaggcagc atgccttgcg ctgaccagcg gcatgatccc caccaagagc
     2761 ggtaccgtta attcctcgaa atccgtttga tattttgaga tgaaatttgt gatcagagcc
     2821 gcttcataga cgggttgggt gatgacgaag tctgctccgt tgcgaatctt ccttctcaaa
     2881 atttccatct cgcgttttgg gtcggacgct gccaggttga gcgcacagcc cacaaagaag
     2941 cttgtgggtt gaccgatatc ggatcctgat tggtcgatgc cctggttgaa acgttgtttg
     3001 atcagcttga ccaggcctga tggtgccagg tcgaaattat ccatcgcctc ggggtaatct
     3061 ccgatggcgg ttgggtcgcc catgatcacg aacacgtttc tgactcccag cgcgtgtgct
     3121 gccagcagat caccctgtac gcgcagcaga tttctgccgc gcgttgggaa gtgcaaaatg
     3181 gtttccacgt tcactttgtt ttgcagtaaa ctgcaaaccg cccacgggct catgcgcatg
     3241 cgcgccatgg ggctgtcagc cacgttaatg gcatctgcgc ctgagtccat cagcatgctc
     3301 gcgcccgcca cgatcttctt ggtagccagc cccctgggcg gttgcatctc aaccgagaat
     3361 gtgaattcct gcgcgctcag tttttgggcg aattcggatg gttcgctggt ttccgtttca
     3421 accgtttctt cctccaccag ctcgagggtt gtgccgttga gagtggctgg tgttgcgtgg
     3481 ttgtcgattg cctggcggat ggaggcgatg tgcgccgggg tggttccgca gcaacctccc
     3541 atcacacctg cccccgcctg ccagaatgcc agcgcgtatt cgccaaagta ttccgctgaa
     3601 gcggggtaaa agatcctgcc cgagatctgt tctggccaac cggcgttggg tttcacccaa
     3661 aataatccat ccggcaccac ctttttcatc tttttcagaa ggcgcagtaa ttggtttggt
     3721 ccgccggagc aattgatgcc gatcacgtct gccccgtatg aaaaaatctt tgttgccact
     3781 ttttgcggat cgtcccccag cagggttcgg tcgtcgcgcg tgaaggtcat cgaagccaca
     3841 accggcatgt caattgccac ttctctcgcc gcgctgatcg cttcgcgcac ttcatagaga
     3901 tctgtcactg tctcgatgac gattaaatcg caccccgctt caaccagtgc ctggatctgt
     3961 tccttgaaaa cttcgtgtgc ctgttctgct tgtactcgcc caaagggagc caggcgaact
     4021 cccagcggtc ccacgtctcc ggcgatcagc acgtctttat acgaagcgcg aacgatgcgc
     4081 cgggcaagtt caacccccgt ctggttgatt tcatcgacca gctcttcca
//
LOCUS       DAOSDS010000157         4121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_7636_length_4121_cov_5.968028, whole genome shotgun sequence.
ACCESSION   DAOSDS010000157 DAOSDS010000000
VERSION     DAOSDS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7636_length_4121_cov_5.968028"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /locus_tag="PLL88_11490"
     CDS             complement(<1..1571)
                     /locus_tag="PLL88_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HOJ02228.1"
                     /translation="MKTNKEKVYDFIKLYASAHENGGVSTQYLAKTLDMLRTNVSSIL
                     NTLYKEGRVLKTNGRPVLYSINRQAELREDRCFEDIIGASGSLKHHVQLAKAAVIYPA
                     KSLNTLITGEHGTGKSFLALIMHRYAITVGILPESAPFVVCDCRDHHNDEGGLIRELF
                     GTDGNGGFFSTVGGGILHIDNAHMLSTGFRSQLCSLIEQSQQNDADLQKTSPMIIMES
                     ESSNRPACEDYTKVMPIVIELPTLDERPLEERMTFIQKFFTIESARAKKSLTINAELL
                     RCLLLYDCHLNIKQLKGDIKRGCAMAYLREHDSKEDNLEIFLYDFEPYVRKGFLNYQS
                     HRAEVERIIPSKYNYTFSESTMEMSAIDQAKIQSSSFYDDIDRKVNELQERGMSKEDI
                     GVLLSSELEELFYQYLNDVSKQVINKEQLVKLVDERVITLVETFLAEAKEELSISFSP
                     AVFYGLCLHINSVIHGNEPRQPMLNQPMQEFAEQNEAEYSMTLQFVEKIEKAFSASLP
                     ADEAIFISMFLRFESV"
     gene            1990..2850
                     /locus_tag="PLL88_11495"
     CDS             1990..2850
                     /locus_tag="PLL88_11495"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOJ02229.1"
                     /translation="MATQEGDVSKKTSFFEWIIRLVKGVMVGVGFIVPGLSGGVLAVV
                     FGIYEPLMKFLGNLKDNFLKNLLFFIPVGIGGVLGVVAFSAVVDFAFANYAAQFTWLF
                     IGFIAGTFPSLFKTAGKEGRKAQHWGLLVAMAVGMLFFMRWMETIQNVNMEPSFFSWI
                     LSGALIGLGVVVPGMSPSNFLIYLGLYQPMASGISHLDFGVIIPLAVGGVVCVLTFAR
                     LVSWLFKKSYAFMYHLILGIVVGSTLAIIPAGVSGWTIAVCVLLFAFGAACSYGLAKL
                     DEKYPHESII"
     regulatory      3071..3208
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3328..>4121
                     /locus_tag="PLL88_11500"
     CDS             3328..>4121
                     /locus_tag="PLL88_11500"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ02230.1"
                     /translation="MLRKYLLPILFVVALSLNACTASAPVATEAPTSAVTEASSISAF
                     PMTVIDGNGREVTIDSAPARIISLSPSNTEILFAVGAGALVVGDTEYCDYPAEAVSLT
                     KVGGYSADSISIETIVSLKPDLVIAEGSSQTTVIEALEQANIKVLAINSKSFEDVYAN
                     IELIGKVTDNETEAIALVDEMKARVSAVTEKISSVPEEEHPTVFWEIWDEPLMTAGPN
                     TFVGQMIRLAGGVNIFAELTEDWPAVSAEEIVQKNPAVIMGPDSHG"
BASE COUNT          980 a          904 c         1010 g         1227 t
ORIGIN      
        1 acgctttcaa agcgtaaaaa catggagatg aagatggctt catctgccgg aagtgatgcg
       61 gaaaaggctt tttcgatctt ttccacgaat tgaagcgtca tagaatattc cgcttcattc
      121 tgttcggcaa attcttgcat tggttgattg agcatgggct gcctgggctc attgccgtgg
      181 atcacactgt tgatatgcag acataaacca taaaagaccg ctggagaaaa agaaatcgag
      241 agttcttctt tggcttcggc aagaaaggtt tcaaccagtg tgatcacgcg ctcatccacg
      301 agttttacaa gttgctcttt gttgatcact tgtttcgaga catcattgag gtattgatag
      361 aaaagctctt ccaattcaga actcagcaag accccgatat cttctttgga catgccacgt
      421 tcttgcagtt cgttcacttt ccggtcgatg tcgtcataaa agcttgacga ttgaatcttc
      481 gcctggtcga tcgcgctcat ttccatggtc gattcggaaa aggtgtagtt gtacttcgat
      541 ggaatgatgc gttctacttc agcgcggtga ctttgatagt tcaagaatcc cttgcgaaca
      601 tacggctcaa aatcatagag gaaaatttcg agattgtcct ctttggaatc atgttcccga
      661 agatatgcca tggcacaacc tcgtttgata tcccccttca gttgtttgat atttaggtgg
      721 caatcataca ggaggagaca gcgcaacagt tcagcgttga tggtgagcga cttttttgcg
      781 cgggctgatt cgatggtaaa aaatttctgg atgaaggtca tcctttcttc cagcgggcgc
      841 tcatcgaggg ttggcagctc gattacgatt ggcatcactt tcgtataatc ttcgcaggct
      901 ggacgattcg agctttcact ttccatgatg atcatagggg atgtcttttg aagatcggca
      961 tcattctgct ggctttgttc gatcagcgaa cagagttggc tgcggaaccc tgtgcttaac
     1021 atatgcgcgt tgtcgatgtg aagaattccg cctccgaccg ttgaaaaaaa gccgccattc
     1081 ccatctgtgc cgaataattc tctgattaat ccgccttcat cattatgatg gtctctgcaa
     1141 tcacaaacaa cgaacggtgc gctttcgggc aagatgccaa cggtgatcgc gtagcgatgc
     1201 atgatcagtg ccagaaaact tttccctgtg ccatgttcac cagtaattaa tgtgttcaaa
     1261 cttttagccg gatagattac tgctgccttt gcaagttgta catgatgctt gagactcccg
     1321 cttgcgccga taatatcttc gaaacagcgg tcctctctga gttcagcctg acgattgatg
     1381 ctgtacagaa caggacgccc gtttgttttt agcacccgcc cctctttgta gagcgtgttc
     1441 aatatgctac ttacgttcgt tcgcagcata tcgagcgttt ttgccaggta ctgcgtggac
     1501 accccaccat tttcgtgagc ggatgcatag agttttataa agtcatatac tttttctttg
     1561 ttggtcttca tgggaaggta cctccaaaga aactatacac tatctatact ctattgcatt
     1621 ctatgggttt ttggttcgcg tttcaaatgc tacattgttt attgcacgaa cgagcagaat
     1681 aaacactgag actttatttg tggtaatttg aactccaaat gttgatgcga gctgcgggtg
     1741 atcaggcttt ttggttaaat ttcgtaaaag tgtattactt cggtagtgaa aaatgcccac
     1801 agtgtataaa agcgccggac gaattcacga attccgttgg tttcatggat tccaatgggt
     1861 ttaccagcat gatctccctc ctgacgatgg gttcatgctc catgaacttt gattggctat
     1921 ttgatttctg ccaaatcaag tatactatgc aggttcaatg aaataacgaa tctctcaagg
     1981 agtaatcata tggcaactca agaaggggat gtttcgaaaa aaacttcttt ttttgaatgg
     2041 atcattcggc tcgtcaaagg ggtgatggtg ggcgtcggat tcatcgtacc cggtttgtca
     2101 gggggtgtgc tggcggtggt gttcggcatc tacgaacctt tgatgaaatt tctgggaaac
     2161 ctcaaagaca attttctgaa aaacctgctc tttttcatcc cggtgggaat cggcggtgtt
     2221 ttaggcgtgg tggctttttc ggcggtggtc gattttgcct tcgccaatta cgcagctcag
     2281 ttcacctggt tgttcatcgg tttcatagcg ggcacttttc cctccctgtt caagactgcc
     2341 ggcaaagagg gcaggaaagc gcagcattgg ggtctgctgg ttgccatggc ggtgggcatg
     2401 ctctttttca tgcgctggat ggagacgatt caaaatgtga atatggagcc atctttcttc
     2461 agttggatcc tgagcggtgc gctgatcggg ttgggggtgg ttgtgccggg catgagccct
     2521 tccaattttc tgatctatct gggtctttat caaccgatgg caagcggaat ctctcatctt
     2581 gatttcggcg tgatcatccc tctggcagtg ggaggggtgg tttgtgttct gacgtttgcc
     2641 aggcttgttt cctggctgtt caaaaaatct tacgcgttca tgtatcattt gatcctcggt
     2701 attgtcgtag ggtccactct ggcgattatc cccgctgggg tgagcggttg gacgatcgcc
     2761 gtgtgcgttc tgctgttcgc ttttggcgca gcctgttcct acgggctggc gaagctggat
     2821 gaaaaatatc cgcacgaaag cattatttaa ggtttattgt caagcgaatg agtcatgacc
     2881 atcttcaaag cgattggtca tgactcatgt tttttcgtat ccgataaagt aactgcttcg
     2941 actcgagttt tcaccccaat tctttaagga tgttgtggtc ctgacatctt atcggctcca
     3001 aattcgtacg gagcatggct ttgcttgcca cattaatttt gttctttgta gtacaataca
     3061 gcttgtgctg acaagtgcat agtgttgagg tgcctgttgc ccggaagcta gggcgtaaag
     3121 gtggaagctg gttcaagtcc agcgctgtac cgcaactgta agtctttttt gacaagccag
     3181 gataccttcc tcaacaaaac tctttttgac ccttctcgga ttaaggaggt agagtatggc
     3241 aaatactttt tcatcataaa acataaccca acccggaagg gaaggttatg tttttttaac
     3301 cccaccatta aaaaacagga gattattatg ttacggaaat atctattgcc tatacttttt
     3361 gttgttgctc tttctttgaa cgcctgcaca gcatctgctc cggtcgcaac agaagcgccc
     3421 acctctgctg taaccgaagc ttcatccatt tcagcgttcc caatgacggt tattgacggt
     3481 aatgggcgcg aagtaaccat cgattccgca cctgcgcgca tcatttccct gtctccctcc
     3541 aatacggaaa tattgtttgc tgtaggagcg ggcgcattgg ttgtgggtga cacggaatat
     3601 tgtgattatc ctgccgaagc ggtttccctg acgaaggtag gcggatattc tgcagattcc
     3661 atcagtatcg aaacaattgt ctcgctgaag ccagacctgg tgatagcgga gggctccagt
     3721 cagacgactg taatcgaagc gttggaacag gctaatatca aagtgcttgc cataaattcg
     3781 aagtcttttg aagatgttta cgccaatatt gagctgatcg gaaaagttac tgacaacgag
     3841 acagaagcca tcgcccttgt tgatgaaatg aaagcgcgtg taagcgcagt tacagaaaaa
     3901 atttccagcg ttccagaaga agagcatccc actgtttttt gggagatttg ggatgaacca
     3961 ttgatgactg ccggtccgaa tacatttgta ggtcagatga ttcgacttgc gggcggcgtc
     4021 aatatttttg ctgagctaac tgaagattgg cccgccgtca gcgctgaaga aatcgtccag
     4081 aaaaaccctg cggttatcat gggacccgac tctcatggtg a
//
LOCUS       DAOSDS010000158         4085 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_7730_length_4085_cov_3.535732, whole genome shotgun sequence.
ACCESSION   DAOSDS010000158 DAOSDS010000000
VERSION     DAOSDS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7730_length_4085_cov_3.535732"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="PLL88_11505"
     CDS             <1..360
                     /locus_tag="PLL88_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02231.1"
                     /translation="RYAPDIVFTHTQADLHQDHSTVTMETLRAFRGISVFGFDVIRSS
                     NGFFPNFLVKIDEVDVEKKVQALAAYSTYKDKYYFSPEITKATLIRNGALAESRYAEG
                     FEIFRIIGSFGEAVQKE"
     gene            complement(387..770)
                     /locus_tag="PLL88_11510"
     CDS             complement(387..770)
                     /locus_tag="PLL88_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HOJ02232.1"
                     /translation="MMDDLYREMILEHYKNPIHKGNLELHNHDFEDENPLCGDFIHID
                     LQVDEDGTIRDAAFSGHGCAISQASADILLDTIIGKKVDEVRNMEKQDLLDLLGIDLG
                     PVRLKCALLPFKVMKAALYGMEKDE"
     gene            complement(780..2003)
                     /locus_tag="PLL88_11515"
     CDS             complement(780..2003)
                     /locus_tag="PLL88_11515"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOJ02233.1"
                     /translation="MLDPFAIRRDFPVFQHKIHGTKELVYLDSAASSQKPQPVLDAMD
                     SLYQESFANIHRGIHALAEEATARYENARQRVAKFVHAGDAVETVFTRNTTESINLVA
                     YAWGRKFLKAGDRILLTEMEHHSNLVPWFILAKEKEILIDFIPVTEKGFLDLDAYQVL
                     LQKNPKLVAFTHMSNVLGVVNPAQEMSAQAHRVGALVLVDGAQAVPHFPVDVTQMDAD
                     FYAFSAHKMCGPTGIGILYGKQNLLEEMPPFLGGGDMIKKVTFEGFIPNELPYKFEAG
                     TPAIAEAVGFGAAVEYLEKIGMQEIQAHEEHITRYAYARLSEIQNLHILGPSAEERGG
                     VISFVLDGIHPHDVAQILDSDGVAVRAGHHCAMPLHQKLAIPASVRASFYLYNTTSDV
                     DALIEGIHKVKKIFA"
     gene            complement(2013..2279)
                     /locus_tag="PLL88_11520"
     CDS             complement(2013..2279)
                     /locus_tag="PLL88_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02234.1"
                     /translation="MASTPFMRPPVFEEIYEVGDMVEANCDHDKGKDRVKGWLKGTVV
                     QVDSKLIAVQFKENVYLTDGWMVPDHILWFPGDSPNLRKKSKIK"
     gene            complement(2321..3646)
                     /gene="sufD"
                     /locus_tag="PLL88_11525"
     CDS             complement(2321..3646)
                     /gene="sufD"
                     /locus_tag="PLL88_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOJ02235.1"
                     /translation="MKSATRIVEKEEQIGEFPFVNVFQPHSGNSEHLSELRVQALDLY
                     RSFPFPNTHTPGWRKISLQELQPGGFRLPPAEPLAIKPPQSVDHLKEVVFTQQICSEG
                     GNALFIPDTLPEGMVCGSTSDAGVAIPAAVLEKIGRIVPPGNGKFAAFAHAFSHATSV
                     IYVGAEVNASTPIFFQRNHEGNHIASPATVMIYVARNASATLIQQNRNVNPLDKTFNA
                     GVTEIYLEEGASLDLLDLNMMSDKNWDFQEQKAILEKNATLNWFTLQAGSGFSKSNLQ
                     VDLVGRGGQALVTGLFLPTGKQRFYMDTAQNHLEANTASDLMYRGVADGESQSRWEGM
                     IYVDKRARGADGYQANNNLILSNSAKVDAIPGLEIMTDDVRCTHGVTITNIDAEQQFY
                     LTSRGISEKEGRDLIVGGFMQKALDRIRSEHIKRMIFEEFISRINKSVL"
     gene            complement(3648..>4085)
                     /locus_tag="PLL88_11530"
     CDS             complement(3648..>4085)
                     /locus_tag="PLL88_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HOJ02236.1"
                     /translation="RNQVQDTGGRMLHLAESTSSKITSKSISKDGGRASFRGLVYVDK
                     KAKFTKSNAVCDALILDGESRSDTYPDIQTHAENVSIGHEASVSKVGEEQLFYLTSRG
                     LSEEEATTMVISGFIEPLVKELPMEYAVEMNRLIQLQMEGSVG"
BASE COUNT          985 a         1080 c          920 g         1100 t
ORIGIN      
        1 agatatgcgc cagatatcgt gttcactcat acccaggcag acctgcacca ggatcacagt
       61 acggtgacca tggaaacctt acgcgctttc cgtggaatta gcgtgtttgg gtttgacgtc
      121 attcgcagta gcaatggatt tttcccgaat tttctggtca agatcgacga agtcgatgtt
      181 gagaaaaagg tgcaagcact ggcagcctac tctacgtata aagacaaata ttatttttct
      241 cctgagatca ccaaggcaac cttgatccgc aatggagctc tggcagagag ccgctatgcc
      301 gaaggattcg agatattccg cataatcggg tcatttggag aagcagtaca aaaagaatga
      361 aaaaaaccgg aaaatattcc ggttttttat tcgtcttttt ccatgccgta cagtgcagct
      421 ttcatcactt tgaacggcag caatgcacat ttcaaacgta ccggtcccag gtcgattccc
      481 agcaggtcta gcaggtcttg cttttccata ttccgcacct cgtccacttt tttccctatg
      541 atggtatcga gcagaatatc ggcagaagcc tgagaaatcg cgcagccgtg cccgctgaac
      601 gctgcgtccc gaatggtccc gtcctcatcc acctgcagat cgatgtgaat gaaatcaccg
      661 cataacggat tctcatcttc aaaatcatga ttatgcagtt ccaaattgcc cttatggatg
      721 gggtttttat agtgctccag gatcatttct cggtacaggt catccatcat aatcaccaac
      781 tatgcgaata ttttctttac tttgtgaatt ccttcgatca acgcatcaac atcagaggtc
      841 gtgttataca aataaaaact ggcgcgtaca gaagccggta ttgccaattt ctgatgcaat
      901 ggcatggcac agtgatgccc ggcgcgcacc gcgacaccgt ccgaatcgag gatttgtgcc
      961 acatcgtgtg gatggatgcc atccagtaca aatgaaatca ccccgccccg ttcttcagcc
     1021 gaaggaccaa ggatgtggag gttctggatt tcactcaacc ttgcataagc gtaccgggta
     1081 atatgctctt catgtgcctg gatctcttgc atgccaatct tttccaggta ttccaccgca
     1141 gctccaaaac cgaccgcttc tgcgatggcg ggtgttccgg cttcgaactt ataaggaagc
     1201 tcattgggaa tgaatccctc aaaagtaacc ttcttgatca tatcccctcc acccaggaaa
     1261 ggaggcattt cttccaacag gttttgcttt ccatagagaa tcccgatgcc ggttggtccg
     1321 cacattttat gggctgaaaa ggcgtaaaaa tcagcgtcca tctgcgttac atccaccggg
     1381 aagtgtggga ccgcttgtgc accatccacc agcaccaacg cacccacgcg gtgtgcctgc
     1441 gcactcattt cttgcgcggg gtttaccacc cccagcacgt tcgacatatg cgtgaaagcg
     1501 accaatttcg ggtttttctg cagcagcact tgataagcat ccaggtccag aaaacctttt
     1561 tctgtcacag gaataaaatc gatcaggatt tctttttctt ttgccaggat aaaccagggc
     1621 accaggttgg aatggtgttc catctccgtc agcagaatgc ggtcacccgc tttcaggaat
     1681 ttccgtcccc aggcatatgc caccagattg atggattccg ttgtgttgcg cgtgaacacc
     1741 gtttcaacgg cgtcacccgc atggacgaat ttggctacac gctggcgggc attttcatag
     1801 cgggcagtgg cttcctcagc caacgcgtga attccacgat gaatattggc aaagctctct
     1861 tgatataaag agtccatggc gtccagcaca ggctgtggct tctggctgga ggctgctgaa
     1921 tccagataaa caagctcttt cgtaccgtgg atcttgtgct gaaagaccgg aaaatccctg
     1981 cggatggcaa aaggatcaag cattctgagt ctctatttta ttttgctttt cttacgcagg
     2041 ttcggtgaat cgccgggaaa ccagagaatg tgatctggca ccatccaccc gtctgttaaa
     2101 tagacgtttt ctttgaactg aactgcgatc agtttggaat caacctgcac aaccgttccc
     2161 ttcaaccaac ccttgaccct atccttccct ttatcatgat cgcaattcgc ttcgaccatg
     2221 tcgccgactt catatatctc ttcgaaaacc gggggtctca tgaacggtgt acttgccata
     2281 gaaaagttct ccttgaacac aaaattttca tattctagca ttaaagcaca cttttattaa
     2341 ttctagatat gaattcttcg aagatcatgc gtttaatgtg ctctgatctg atgcgatcca
     2401 atgctttttg catgaaaccg ccaacgatca ggtctcttcc ttctttttcg gatatgccgc
     2461 gcgaagtcaa atagaattgc tgctcagcat cgatgttcgt gatggtgacc ccatgagtac
     2521 agcgcacgtc atctgtcatg atctccagac ccgggatggc atccactttg gctgaattgc
     2581 tcaagatcaa gttgttgttt gcctgatacc catctgcgcc ccttgcccgt ttatccacgt
     2641 aaatcattcc ttcccatcgg ctctggcttt ccccatccgc taccccgcga tacatcagat
     2701 cgctggcagt attggcttcc aaatgatttt gcgcggtgtc catgtagaaa cgttgttttc
     2761 ccgtcgggag aaacaagcca gtgaccaatg cctggccgcc gcgccccacc agatctacct
     2821 gcaaattgga ctttgaaaat cctgaaccgg cttgcaaggt gaaccaattc aacgtggcat
     2881 ttttttcaag aatagccttc tgctcttgaa aatcccagtt cttgtcagac atcatgttca
     2941 gatcaagcag atccaggctt gccccttctt ccagatagat ctcagttaca ccggcgttga
     3001 aagtcttgtc gagagggttg acgttcctgt tctgctggat caaagttgcg gaagcattcc
     3061 gcgcaacata gatcataacg gtcgcaggac tggctatgtg gttcccctca tgatttctct
     3121 gaaaaaagat cggcgtgctt gcgttcacct ctgcacccac gtagattacc gaagttgcat
     3181 gggaaaatgc gtgcgcaaag gcagcgaatt ttccgttgcc gggcggaacg atccgcccga
     3241 ttttttccaa tacggcagca gggattgcca cgcctgcatc tgaggtcgat ccacacacca
     3301 tcccttcagg caacgtatcc gggataaaaa gcgcattacc cccttctgag catatttgtt
     3361 gggtgaagac cacctctttg agatggtcaa cggattgcgg cggtttgatc gccagtggtt
     3421 cggctggggg caaccgaaaa cctccgggct gcaattcttg cagtgatatc ttccgccaac
     3481 ccggcgtgtg agtgttcgga aacgggaagg aacgatacag atccagagct tgaacgcgta
     3541 gctcactgag atgttcagaa ttaccggaat gtggctggaa aacgttcaca aacggaaatt
     3601 cccctatttg ttcttccttt tccacaatgc gagtagcgct tttcatgtta tcccaccgat
     3661 ccttccatct gtagttggat caatcggttc atttccaccg cgtattccat gggcaattct
     3721 ttcaccaaag gttcaatgaa tcctgaaatg accatcgtgg tggcttcttc ttcgcttaag
     3781 ccacggctgg tcaaataaaa aagctgttct tcccccacct tcgataccga cgcctcatgc
     3841 cctatagaga cgttctcagc atgcgtctgg atatcagggt acgtatcaga acgggattct
     3901 ccgtccagaa tcaaagcgtc gcaaaccgcg ttggatttgg tgaattttgc tttcttatcc
     3961 acgtacacca gcccacgaaa cgatgctctc cctccatctt tgctgataga tttggatgtg
     4021 atcttgctgg aagtgctctc tgccaaatgc agcattctac cgccggtatc ctgcacttgg
     4081 tttct
//
LOCUS       DAOSDS010000159         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_8020_length_3969_cov_4.831375, whole genome shotgun sequence.
ACCESSION   DAOSDS010000159 DAOSDS010000000
VERSION     DAOSDS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8020_length_3969_cov_4.831375"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="PLL88_11535"
     CDS             <1..336
                     /locus_tag="PLL88_11535"
                     /inference="COORDINATES: protein motif:HMM:NF023598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase MtaB domain-containing
                     protein"
                     /protein_id="HOJ02237.1"
                     /translation="ALALRDLFVESDSTLDVQAYVLRPDVVLEISRQIIQESTAYLRT
                     RAAARVALAHLRKASEDQQITLSRVELRWLDTLSKQADQLPESEEEFIDQMKRQIDVS
                     KVNLSEYDL"
     gene            383..1018
                     /locus_tag="PLL88_11540"
     CDS             383..1018
                     /locus_tag="PLL88_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HOJ02238.1"
                     /translation="MSDILSEIYQSVLEGESTTVAEKINLAMAEGIAAETILNNGMVA
                     AMSEVGRLFEEGEYFVPEMLVSARAMQEGLSVLKPFLAKADVRSKGKVIIGTVAGDLH
                     DIGKNLVGIMLEGAAFEVVDLGTDVAAEKFVEAVKTYNPHVVAISALLTTTMNNMKSV
                     IQAIEAAGLRKNVKIIIGGAPITAQFAESIGADGYSPDASKAVALVRGLIQ"
     gene            complement(1070..2083)
                     /locus_tag="PLL88_11545"
     CDS             complement(1070..2083)
                     /locus_tag="PLL88_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ02239.1"
                     /translation="MTATIKDVARMAQVSIKTVSRVVNNEPHVAEDMRARVQNAIDLL
                     GYAPNISARRLVQRRSYMLCILLHTSGFYQSALINKTLEVGYEYDYDILIQTYFPSQA
                     RSKKKLAGLINERRIDGLITTPPCDTDEFVLDLVRNSHIPLVQISPYNPSPEVPAITA
                     DDFQGAYLMTEHLINLGHRNIAFLKGPRNHRTSLERLLGYRRALEHYHIAPKSQLELD
                     SEFNFDGGYTAAKIAMNQVEPPSAIFGGNDEAAFGAIYALQEMGFSVPQHISVCGFDD
                     LANSKNVWPGLTTVHHPVNEIAEQALSMLVTLLEGKEIEERERVIPSHLVIRASTSHP
                     FSH"
     gene            2459..3439
                     /locus_tag="PLL88_11550"
     CDS             2459..3439
                     /locus_tag="PLL88_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ02240.1"
                     /translation="MGNPKQPVVIFSNVFKSFGTVQALRGISFELLPGEAFAILGHNG
                     AGKITSIRLILGLLKPDSGAVRLFGHDPYPEDELNRKRRQHIGVVQEEDRLYLQMSSE
                     ENLLFWLALYGVQKQQRKERAFSALSWVGLEKRGQGKVGTYSKGMRRRLAIARALVLE
                     PQVLILDEPTVGLDPEARVEVRELLERLIHREGLTLLMTSHDLDEVEKLCKRLIVIDH
                     GQMSLAGTLSELRAGQQPELSIKLVREGEGIIHESLFQELTALPFVTRLELNGNMLTV
                     MLNKGEEEFISQVVARLAGAGVGIAEVELYQRSLEEIYLQATANHKGNSD"
     gene            3451..>3969
                     /locus_tag="PLL88_11555"
     CDS             3451..>3969
                     /locus_tag="PLL88_11555"
                     /inference="COORDINATES: protein motif:HMM:NF024141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ02241.1"
                     /translation="MNPMLILFRKELGIAKRPTYWASCLLMGALMIWIYVHTPIETIK
                     TIAQTSPQIADYLATSWPQMFALLQPLWLFLLTWLLLSSEVFTMEKTDGRLEMLLTTP
                     LHLREIWVAKCLALLVLVYPYVLLVSCLVFGLQSYVWPVQLGLDFHISTVTLSMAVCV
                     GPLLAFGLAALLG"
BASE COUNT          992 a          845 c         1068 g         1064 t
ORIGIN      
        1 gccttggctt tgcgcgatct gttcgttgaa tcagattcaa cgctggatgt gcaagcttat
       61 gtgctgcgac cggatgtggt actggaaatt tcacggcaga tcatccaaga atcaaccgct
      121 tacctgcgta cccgagccgc tgccagggtg gctctggcac acctgcgcaa agccagtgaa
      181 gatcaacaaa taaccctttc acgagtagaa ctgcgttggc tggataccct atccaaacag
      241 gcggatcaac tgcccgaatc cgaagaggaa ttcattgatc aaatgaaacg tcaaattgat
      301 gtgtccaaag tgaacctatc agagtatgat ctataactag gcaggtcaat acgttggaat
      361 ccatgaatga aagtgagctg gcatgagtga tattctttca gaaatctatc agagcgtcct
      421 ggaaggcgaa agcaccacgg tcgctgaaaa gatcaatctg gcaatggcag aaggaatcgc
      481 tgcagaaacc atactcaaca atggaatggt cgctgcaatg agcgaggtag gacgtttatt
      541 cgaagaagga gaatatttcg tgcctgagat gttggtctct gccagagcca tgcaagaggg
      601 attatccgtt ttaaaacctt ttctggcaaa agctgatgtt cgatcgaaag ggaaggtgat
      661 catcggaacg gtagccggtg atctgcacga tatcggaaaa aacctggtcg gcattatgct
      721 tgagggcgct gctttcgaag tggttgacct ggggacggat gttgctgctg aaaagttcgt
      781 ggaggctgtc aagacgtaca atcctcacgt ggttgctatt tctgcattgc tgaccaccac
      841 catgaacaat atgaaatcgg tcattcaggc tattgaagca gcgggattgc gcaagaatgt
      901 gaagattatc attggtgggg cacccattac agcccaattt gctgaatcca tcggagcaga
      961 tggatattca ccggatgcaa gcaaagctgt ggcgcttgtg cgagggctga ttcagtagct
     1021 gggggtcaaa gcgccctgtg cacgttgatc agatccctgc attgcctgtt caatgcgaga
     1081 atggatggct ggttgatgca cgaattacca gatgggaggg aatcactctt tcccgctcct
     1141 caatttcttt cccttctaac agtgttacga gcatggagag ggcttgttct gcgatctcat
     1201 ttaccggatg gtgaaccgta gttagtccgg gccaaacgtt ctttgaattg gcgagatcgt
     1261 cgaaaccgca aacggatatg tgctgtggaa cagagaatcc catttcctgc agtgcataga
     1321 ttgctccaaa agccgcttcg tcattcccgc cgaaaatagc ggagggtggt tcaacctggt
     1381 tcatggcaat cttggcggct gtatatcctc catcaaagtt gaattcagaa tccaactcca
     1441 actggctttt tggggcgatg tggtaatgct ccagagcccg gcgatatccc aacaggcgtt
     1501 ccagactggt tcgatgattg cgaggtcctt tgaggaatgc gatattgcga tgaccgagat
     1561 tgatgagatg ttcggtcatc agataggcac cttgaaaatc gtctgcggtg atggcaggga
     1621 cttcaggcga tggattgtag ggcgatatct gcaccagggg aatgtgggag tttcgcacca
     1681 ggtccagcac gaactcgtct gtatcgcagg ggggagtggt gatcagaccg tcaatacgcc
     1741 gttcgttgat caaaccagcc aattttttct ttgagcgtgc ttgcgatggg aagtaggtct
     1801 ggattagaat gtcgtaatca tattcatacc ccacttccag ggtcttatta atcaaggcag
     1861 attggtagaa gccgcttgtg tgaagcaaaa tgcataacat gtaagaacgg cgctgtacca
     1921 accgacgagc agaaatgttg ggcgcatatc ccaacagatc aattgcgttt tgtacacggg
     1981 cacgcatgtc ttcggctaca tgcggttcat tattgaccac ccgcgaaacc gtcttgatgg
     2041 agacctgtgc cattctggcg acgtctttaa tagttgcagt caattgatgc tctcgtataa
     2101 caagggttta ctataggatt tattataaca attctatata actagatagt cgggcacgtg
     2161 ctatttccaa aaaaggtgac gttctggcaa aattcaagca atatctgctg ataatactcg
     2221 gagctggagt cgtctgcatt ctgccacttt tctatccgga gcgctttacc agtagcgacg
     2281 ctgacttagc tgtgcatctg gatacctgtt tccatgatgg ttggatctgt tacctttatt
     2341 gaatagtagt tttttgaggt aatgctcctt ttggtagctt gcccactgcg tgataaaata
     2401 gtatgaagtt atctcatagt aagaaaacgt ggagggaatt tgaggcaatg agagaacgat
     2461 gggtaaccct aagcaacccg tagtaatctt ttctaatgtt ttcaagagct ttggaacagt
     2521 gcaggcactg cgtggtatct cgtttgagct actgcccgga gaagctttcg ctattctggg
     2581 gcacaatgga gctggcaaaa ttaccagcat tcgcttgata cttggtttgc tgaaaccgga
     2641 tagtggtgct gtgcgtcttt tcgggcatga tccttatccc gaggatgaac taaaccgaaa
     2701 gcgacgtcag cacatcggcg tcgtccagga agaagatcgt ttgtatttgc agatgagtag
     2761 cgaagaaaac ttattgttct ggcttgctct ctatggtgtg caaaaacagc agcggaagga
     2821 acgggcgttt tctgcgctgt cgtgggtagg tctggagaaa agggggcagg ggaaagtggg
     2881 cacttattcc aaggggatgc gccggcgatt agccatcgca agagccctag tgttggagcc
     2941 tcaggtgctc attttagatg aacctacggt cggtctggat ccggaggcac gagtcgaggt
     3001 tcgcgaactc ctggagaggt tgatacaccg cgaggggctc acactgctca tgacttccca
     3061 tgatctggat gaagtagaaa aactttgcaa gagactaata gttattgatc atgggcagat
     3121 gtcgcttgct ggcaccctct ctgaattgcg tgccgggcag caaccagagc tatctatcaa
     3181 attggtgcgc gagggggagg gaattattca cgaatctctg tttcaagagt tgaccgcttt
     3241 gcctttcgtg acgcggttgg aattgaatgg caatatgctt acagttatgt tgaacaaagg
     3301 tgaggaggaa tttatcagcc aggtggtcgc taggctagct ggggcaggtg ttgggattgc
     3361 ggaagttgag ctttatcaac gctcgctgga agaaatttac ttacaggcca ctgcgaacca
     3421 caagggaaac agtgattaag gaaaattcaa atgaatccga tgctgatctt gtttcgtaaa
     3481 gaacttggta ttgccaagcg gccaacatat tgggcgagtt gcctgctcat gggagccttg
     3541 atgatctgga tttatgtgca cactcctatc gaaaccataa aaacaatagc tcagacttcc
     3601 cctcaaattg cagactacct agcgacaagt tggccacaga tgtttgcttt gctacaacct
     3661 ttgtggttat ttctactgac ctggttgctt ctttcctcag aggtatttac gatggaaaaa
     3721 acagatggtc gcttggagat gctgctcacc acaccgctgc acttacgtga gatttgggtt
     3781 gcaaaatgtt tggcgttgct cgtgcttgtt tatccctatg ttctcttggt gagctgccta
     3841 gtatttggtt tacagtccta tgtctggcct gttcagttag ggttagattt ccacattagc
     3901 actgttacgt tgtcaatggc tgtctgtgtt ggtccgttgt tggcttttgg attggcagcc
     3961 ttgctcggt
//
LOCUS       DAOSDS010000160         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_8106_length_3937_cov_5.648892, whole genome shotgun sequence.
ACCESSION   DAOSDS010000160 DAOSDS010000000
VERSION     DAOSDS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8106_length_3937_cov_5.648892"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLL88_11560"
     CDS             complement(<1..528)
                     /locus_tag="PLL88_11560"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOJ02242.1"
                     /translation="MSRHPNIVVVGSFIMDLVFRTYRRPHKGETVIGKEFGMFTGGKG
                     FNQAIAASRLGANVTMVGCLGTDIFGDSFVSSMQKEGVNTRFVIRNDNIGTGVASPVI
                     YESEGDNSIIIIPRANQTLKPAHIDAASAAIKEADLLMLQLEIPVETSFYAANIAHAA
                     GVRVMLNPAPFAPLPS"
     gene            complement(554..1150)
                     /locus_tag="PLL88_11565"
     CDS             complement(554..1150)
                     /locus_tag="PLL88_11565"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ02243.1"
                     /translation="MESNENISTAKQVILSEAVALNNLADLIDDAFMNAVEMILACEG
                     RVIFSAVGKPGHIGRKLAATFASTGTPAFFVHAAEAAHGDLGMITAKDLVFLLSHSGS
                     SDEVIHLIPYIRRIGAKIIAITSQKNSPLANSADLVLLTGVEKEADPRGQAPTTSSTA
                     LLALGDALAITVSMQRGFTRKDFRNLHPGGALGRQTNT"
     gene            complement(1171..1602)
                     /gene="fucU"
                     /locus_tag="PLL88_11570"
     CDS             complement(1171..1602)
                     /gene="fucU"
                     /locus_tag="PLL88_11570"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: protein motif:HMM:NF011949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HOJ02244.1"
                     /translation="MLVGISPLLSPDLLATLSRMGHGDEIVLADAHFPGDSMNTHVIR
                     ADGLTIVPLLDAIFPLFVLDKSFDYPLMMMAPTPGDPIDVQVEIAYMQSARKVWPDTP
                     EIQKIERFAFYDRARKAFAVVMTGDTATYANIILRKGVTPL"
     gene            complement(1639..2658)
                     /locus_tag="PLL88_11575"
     CDS             complement(1639..2658)
                     /locus_tag="PLL88_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ02245.1"
                     /translation="MTNNLKRTTVHDVAKKAGVSTATVSRVINDNDAVSYELKAQVQK
                     VIDELGYRPNKAAHHLRSGKVKRVGVLFADIRNPFFTSVLAGIENSLHEAGYALILGN
                     SNEDAKIENMQLQAFLDEGVAGIILSAVTNSGSPYQQVLDAGIPILAIDRIPGNLKVD
                     SISIDNVTAAKQAALHLINLGHQKIAFIGGPEHISTAHLRKKGYLEALEEHPSRQPII
                     ELGDFRQSGGYQATKNILAAHDDISAFLVANNLMTLGALQAFNEKKILIPEQIALVGF
                     DDMPWATSLQPPLTVVAQPTYDMGKIAARLLLDRIKNPDSPIQQVILETQLIIRESCG
                     GLRTA"
     gene            2986..3876
                     /locus_tag="PLL88_11580"
     CDS             2986..3876
                     /locus_tag="PLL88_11580"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ02246.1"
                     /translation="MKNANPFHGIICPILTPFDKKGNIDFVALQRFIEFLVSEKMPCL
                     MIGGTTGEGPLLSVEERKQLSEAAVQFVAKRMKIIVHVSCLSTRESLALTEHAQELGV
                     DGISAITPFFFSYSEGEVYRFYEAIAKTAPEIPISLYCFPGNAKYEIKGSLLEQIIKN
                     LPNVKAIKSSNPDLIRLYEYVTIAQNDFSVLCGVDALTLPALTLGACGQVSGNSNIFP
                     QPFIKLYDSYHRGDLETARECQVAINAVRAVLKDSIAHFKAASEIQGVPVGNPRLPIQ
                     PLSQSEKAKLSIDIASLKSS"
BASE COUNT         1065 a          875 c          931 g         1066 t
ORIGIN      
        1 agaaggaagt ggcgcaaagg gtgccgggtt caacatcacc cgcactcctg cagcatgcgc
       61 gatgtttgcg gcatagaaac ttgtttcgac gggaatctct aattgaagca taagcagatc
      121 ggcttcttta attgcagcag aagcggcatc gatatgtgcc ggcttaaggg tttgattggc
      181 tcgtggaatg ataatgatgc tgttatctcc ctctgattca taaattaccg gagatgccac
      241 accggtgcca atgttgtcgt ttcgaatcac aaacctggtg ttcactccct ctttttgcat
      301 ggaggaaaca aacgaatccc cgaatatgtc tgtaccgagg cagccgacca tggtgacatt
      361 tgcgccaaga cgcgatgctg cgatcgcttg attaaatcct tttccacccg taaacatccc
      421 gaattctttc cctataaccg tctctccttt atgcggtcgt cgataggtgc gaaaaacaag
      481 gtccatgatg aaacttccaa caacgacgat attaggatgg cgactcataa agcattctct
      541 cttttactgg tatctacgta ttggtttggc gccccagagc accgccggga tgcagattgc
      601 gaaaatcttt tcgtgtaaaa ccgcgctgca tggaaacggt gatcgccaat gcgtcaccaa
      661 gcgcaagtaa cgcagtagaa ctggtggtgg gggcttgacc acgcggatcg gcttcttttt
      721 ccactccagt caataaaact aaatccgcgg aatttgccag cggggagttc ttctgagaag
      781 tgatcgcaat gatctttgcc ccaatccggc gtatgtatgg gatcaaatgg atcacttcgt
      841 cgctgctgcc tgaatgtgat aataaaaaga cgagatcttt tgcagtgatc attcccaaat
      901 caccgtgcgc ggcttctgct gcgtggacaa agaatgcagg cgtgcccgtg ctcgcaaatg
      961 tggcagccag ttttctgccg atatgtccgg gtttgccgac agcagaaaag atgaccctgc
     1021 cctcgcaagc caaaatcatc tctacagcgt tcatgaaagc gtcatcaatt agatctgcca
     1081 ggttattcaa agcgacggct tccgagagaa tgacttgttt ggcagtgctg atgttctcgt
     1141 ttgattccat atggctccat ccataaaaaa ttacaaaggc gtaacacctt ttcgtaagat
     1201 aatgttggca tacgttgctg tgtcgccggt catcacgacc gcaaacgctt tcctggcgcg
     1261 atcgtaaaat gcgaaacgtt caattttttg aatttcagga gtgtcgggcc aaacttttct
     1321 tgccgattgc atgtaggcaa tctcaacctg aacatcgatg gggtctccgg gagtgggagc
     1381 catcatcatc aggggatagt cgaaggattt atcgagtaca aagaggggaa aaattgcatc
     1441 cagcaaggga acaattgtca gaccgtctgc gcgaatgaca tgcgtgttca tgctatcacc
     1501 agggaagtgg gcatctgcca gtacaatttc atcaccatgc cccatacggg acagcgtagc
     1561 aagcaggtcg ggacttaata atggtgaaat tccaacgagc ataaaacctc ctatgaagaa
     1621 attcaaaaaa gtaagcgact aagcagttct caatcctcca catgactcgc ggatgataag
     1681 ctgtgtttcg agtattacct gttggatagg gctatctggg ttcttgatgc gatcgagcaa
     1741 taaacgcgct gcaattttgc ccatatcata agtcggctga gccaccactg tcagaggggg
     1801 ctgcagcgat gtagcccaag gcatgtcatc gaagcctacc aacgcgatct gttcgggtat
     1861 gaggatcttc ttttcattga aagcttgcag agcgccaagg gtcataaggt tgttggctac
     1921 caaaaatgct gaaatatcat catgggcagc cagaatattc tttgtggctt gatatccccc
     1981 agattggcgg aaatcgccca gttctatgat cggttggcgg ctggggtgtt cttcaagggc
     2041 ttccagatag cctttttttc tcagatgggc agtggaaata tgctccggcc cgccgataaa
     2101 cgcgatcttt tgatgaccca aattgattaa atgtagcgct gcttgttttg ctgctgttac
     2161 gttgtcaata gagatggaat ctaccttaag attcccgggg atacgatcga tggcaaggat
     2221 agggatgccg gcatctaaaa cttgctgata tggtgagcct gaatttgtga ccgctgacaa
     2281 gattatccct gccaccccct catcaagaaa agcttgaagc tgcatgtttt cgatctttgc
     2341 atcttcattg gaattgccga ggatcaaggc ataaccggct tcgtgaagag aattctcaat
     2401 acctgccaac acgctggtaa agaaggggtt gcgaatatcc gcgaaaagaa ccccaactct
     2461 tttaacttta ccggatctca ggtggtgggc tgctttattg gggcgatatc ccaattcatc
     2521 aatgactttc tgaacctggg ctttcaattc ataacttact gcatcattgt cattgatcac
     2581 acgtgatact gtggcggttg aaactcccgc tttttttgct acatcgtgaa ccgttgtcct
     2641 cttcagatta ttagtcatat gtaatatttt cacatgtttc agtatctaat gtaatccttt
     2701 acaaaatatc atacaaatga agtcttgtca acatggaaaa aaggatagaa aagatcatga
     2761 ataaggaaaa catagcagaa taaagcgcga aatggcatgg taagaggcgg gcaggctttg
     2821 tgcctggcga gatgttgcgc gcaacttgcc aatttattaa actgttgaca aaaaagaagt
     2881 aatcgtttac actggattga gaaaacgatt acatcgcctc agtgcttcca ctgccgtaca
     2941 ttctataagg agaaaatcac tgtttttgtc gtgtcccatc ctattatgaa gaatgccaat
     3001 ccatttcatg gaataatttg tccgatctta accccttttg ataaaaaggg aaacattgac
     3061 tttgtcgccc tgcaacgatt catcgaattc cttgtatctg aaaaaatgcc gtgtttgatg
     3121 atcgggggta ctaccggcga aggaccgctt ttatctgttg aagaaagaaa gcaattgtcc
     3181 gaagcggcgg ttcaatttgt agccaagaga atgaaaataa tcgtccatgt cagttgtctt
     3241 tcgacaagag agtcgcttgc attaacggaa catgcccagg agttaggagt ggatggcata
     3301 tcagcgatta ctcccttctt ctttagttat tccgaagggg aggtgtatcg cttctatgaa
     3361 gcgatcgcga aaaccgctcc tgagattcca atctctttat actgttttcc gggaaacgcg
     3421 aaatatgaaa ttaagggctc actactggag cagataatca aaaatttgcc gaatgtgaaa
     3481 gcgatcaagt cctcgaaccc ggatttgatt cgactgtatg aatatgtgac gatcgctcaa
     3541 aacgattttt ccgtactctg tggagtcgat gcgctgacac tcccggctct tacattggga
     3601 gcttgtgggc aggtgtcagg aaattccaat atctttcctc aacctttcat caaattgtat
     3661 gattcgtatc atcgtggtga tttagaaacc gctcgggaat gtcaggttgc gattaatgct
     3721 gtacgggctg ttttaaaaga cagcattgcg cacttcaaag cggcttctga aattcagggt
     3781 gttcctgtgg gaaatccgcg cttgcctata cagcctttaa gccaatctga aaaagcgaaa
     3841 ttaagcattg acattgccag tttgaaatca tcataaacca gaatttaaga aggaggtgct
     3901 tctgaataca tttgcctgat ttaaaaacac gttgacg
//
LOCUS       DAOSDS010000161         3935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_8113_length_3935_cov_3.844845, whole genome shotgun sequence.
ACCESSION   DAOSDS010000161 DAOSDS010000000
VERSION     DAOSDS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3935
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8113_length_3935_cov_3.844845"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLL88_11585"
     CDS             <1..452
                     /locus_tag="PLL88_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02247.1"
                     /translation="DDVRNVFKSLFAQRNFVFLAADRNFSNDMSSLTCLVVLPESIPF
                     IQFNPRIENYIKAVKKNLGRNFETIEEEYFEFQGLSAVRLLSAQRAPKTRKDPEPDIK
                     RQHLLYAFRLRRHYWAFDWIADISVFDKYLPVFDQSIKSLVFKEKAR"
     gene            641..964
                     /locus_tag="PLL88_11590"
     CDS             641..964
                     /locus_tag="PLL88_11590"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ02248.1"
                     /translation="MRLLNPIVLLCLQKNSAHGYSLMESLAEYGLSNFDVSMLYRALH
                     QMEAFGWVSSALEEESSQGPPRRVYHITSLGENILKEYISDLEKHLEHTQKIIQDYKA
                     LTNQH"
     gene            985..1341
                     /locus_tag="PLL88_11595"
     CDS             985..1341
                     /locus_tag="PLL88_11595"
                     /inference="COORDINATES: protein motif:HMM:NF036294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOJ02249.1"
                     /translation="MPAGNKMGPEGVGPMTGRKVGYCAGYTQPGFRSAGFGYGRMSMI
                     GRGRFHYDPYGRGSFFGHPWEKGGHFAADEFEPQPVSLDEEEHILSQQASRLKSQLDP
                     INAHLEKLQAKKTTEG"
     gene            1375..>1585
                     /locus_tag="PLL88_11600"
     CDS             1375..>1585
                     /locus_tag="PLL88_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOJ02250.1"
                     /translation="MIICLCSEGKTLEDRISPVFGRSPCFLIYDDQLKTLRCIENAAQ
                     GEAHGAGTNAAQIILDESVNVLIANA"
     assembly_gap    1586..1595
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1718..2089
                     /locus_tag="PLL88_11605"
     CDS             1718..2089
                     /locus_tag="PLL88_11605"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOJ02251.1"
                     /translation="MKIAVSSNDNKGLESTVSEHFGRCPFFTMVTVNENTIQKTEVVE
                     NPFYEGHQPFEIPNFLKSQGIQAIVTQGMGGRAIGFFQENAIQSVTGCSGTVKDAVNA
                     YLSGKFFQAAPCSESIAHHHG"
     gene            2106..2987
                     /locus_tag="PLL88_11610"
     CDS             2106..2987
                     /locus_tag="PLL88_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ02252.1"
                     /translation="MQLSIASGKGGTGKTMVAANLAAAVSRHLSSVYFLDCDVEAPNA
                     ALFLQPQIHSVSEVFQFVPRLDEEKCTLCGLCAGNCQFNALVASKSKILFFPELCHSC
                     GGCILICPEQAIAETPARIGTIQKGTSVRMPFFQGKLDIGRPSPVGIIQQMFQEQQKS
                     SANEDHSRITIVDSPPGVSCAVVASTRGSDFVILVSEPTPFGLHDLRLAVQLVRDELK
                     IPFGVILNKSDDRIAIIDEYCAEEGISILQRIPFSRQIAEIYAKGQLLIDVDPIFESL
                     FLELWEKVRTILQKGKP"
     gene            2984..3889
                     /locus_tag="PLL88_11615"
     CDS             2984..3889
                     /locus_tag="PLL88_11615"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ02253.1"
                     /translation="MKQIAILSGKGGSGKTSICAALIDLISQNHSLSMVDADVDAANL
                     NLLIPCTEQTEQSFQGGKKAIIAQESCIRCGICLKYCRFHAVHRTLDDSGLNPRFSID
                     LLACEGCAVCAQQCPSSAISMHPTQDGVWYRSSSEYGECFHAHLFAGHENSGKLVSTI
                     ISAARRYVQNSAVDLILVDGPPGIGCPVIAVCNQSDLALIVTEPTPSGIHDMQRILDT
                     IRYFDLPAFLIINKFDLHLQNTEQILEQCEREDVPVLGQIPFDPQFNEAILHAQPITR
                     YATDSLSNQAIHAIWQQLKSQVLNS"
BASE COUNT         1072 a          965 c          896 g          992 t
ORIGIN      
        1 cagacgacgt ccgcaacgtc ttcaaaagcc tgttcgcaca acgcaatttc gtattcctgg
       61 ctgccgaccg aaatttctcc aacgacatga gttccctcac ctgcctggtc gtgctgcccg
      121 aatccattcc cttcatccag ttcaatccca ggatagagaa ttatattaaa gcggtgaaaa
      181 agaaccttgg tcgcaatttt gagacgatcg aggaagaata tttcgaattt caggggcttt
      241 ccgctgtccg actgctctcc gctcaacgcg cgccaaagac gcgcaaagac cccgaaccag
      301 atataaaacg tcaacatctt ctctacgcgt ttcgcctgcg cagacactat tgggcatttg
      361 attggatcgc cgatatttcg gttttcgaca aatatttgcc tgtgtttgac caaagcatca
      421 aaagtctggt cttcaaagaa aaagcaagat aaggtttgcc tatttttcca aagtttatct
      481 gaattcaata aattattgaa tcttctaaaa ttctaaagtg cagaccgtgc actttttcat
      541 ctccatctat atgcatctta tctattgact atttgcatat agtataatat ttttaggggg
      601 aggaaatatg ctatacggaa gacacagaaa aggacgtgga atgcgtctgc tgaacccaat
      661 tgtgctgctg tgcttgcaga aaaattctgc acacgggtat tcactaatgg agagtcttgc
      721 tgaatatgga ctgagcaact ttgatgtcag catgctttac cgcgctctcc atcaaatgga
      781 agcttttggg tgggtttcct ctgccctgga agaggaaagc agtcagggtc ccccccgtcg
      841 tgtttaccat attacatccc ttggagagaa tattctgaag gaatatattt ccgatctgga
      901 aaagcatctt gaacatactc aaaagatcat tcaagattac aaggcactta caaaccaaca
      961 ttaacaaaag cgaaaggagt cgaaatgcca gcaggaaaca aaatggggcc agaaggtgta
     1021 ggtcccatga caggaagaaa agtgggatac tgcgccggtt atacgcaacc cggttttcgt
     1081 tcagccggtt ttggatacgg cagaatgtcc atgatcggtc gaggaagatt tcactatgac
     1141 ccctatggaa gagggtcctt cttcggacat ccatgggaaa agggcggcca ttttgcagca
     1201 gatgaattcg aaccacagcc cgttagccta gatgaagaag aacatattct cagccaacaa
     1261 gcctcccggt taaagagcca gctcgatccg atcaacgctc atctggaaaa actccaagcc
     1321 aaaaagacga cagaggggtg accgttctga cccatccagg tttggagtga attcatgatc
     1381 atttgcttat gcagcgaagg gaaaacactt gaagatagga tcagccccgt gtttggtcgt
     1441 tctccctgtt tcttaattta tgatgatcaa ttgaaaaccc tgcgctgtat tgaaaacgct
     1501 gcccagggtg aagcgcatgg tgcaggaacc aacgccgctc agatcattct ggacgaaagc
     1561 gtgaacgtgc tgatcgcgaa cgcacnnnnn nnnnnaaaaa aattttgagc atcggcaaga
     1621 taaagatttt tcgtcagaca ggagaaacac cccaaaaagc actggcagct tttcaagaag
     1681 gaaaattaga aaaattataa agaaaatgga gttgagtatg aaaattgcgg tatcatccaa
     1741 tgacaacaaa ggattagaaa gcacagtcag cgaacatttc gggcgttgcc cattcttcac
     1801 aatggttact gtgaatgaga ataccatcca aaaaaccgaa gtggttgaaa acccctttta
     1861 cgaaggtcat caaccctttg aaatccccaa cttcttgaag tcacaaggaa ttcaagctat
     1921 cgtcacgcag ggtatgggtg gtcgcgccat cggctttttc caggaaaatg ccatccagtc
     1981 ggtcaccggc tgcagcggaa ccgtcaaaga cgccgtgaac gcatatcttt caggaaaatt
     2041 ctttcaagct gctccatgca gcgagagcat cgcccatcac cacggataaa acctgttcat
     2101 catccatgca gctttccatc gccagcggga aaggtggtac cggaaagact atggtggctg
     2161 ccaatctggc agcagccgtc agccgccacc tttcttctgt ttattttctt gattgtgatg
     2221 tagaagcgcc caatgcagct ctgtttctgc aaccgcaaat tcattctgta agcgaggttt
     2281 ttcaatttgt tcctcggctc gatgaagaaa aatgcaccct gtgcgggctt tgtgccggaa
     2341 actgccagtt caatgctctg gttgcttcaa aatccaaaat cttattcttt ccagaactgt
     2401 gccattcctg cggcggctgc atcttgatct gccccgaaca agccatcgcg gaaactcccg
     2461 cacgcatcgg gaccattcag aaggggacca gcgtccgcat gcccttcttt cagggaaagt
     2521 tggacatagg acgacccagt ccggtcggga tcatccagca gatgttccag gaacagcaga
     2581 aatcttctgc taatgaagat cattcaagaa taacgatcgt tgattctcct cccggagttt
     2641 cctgtgcagt cgttgccagt acgcggggtt cggactttgt catacttgtc agtgagccca
     2701 ccccctttgg cttgcacgat ctgcgtctgg ctgttcaact ggtgcgcgac gaattgaaga
     2761 taccttttgg agtgatcctg aacaaatccg atgatcggat agcaatcatc gatgaatact
     2821 gtgcggaaga aggcatctct attctgcagc gcattccttt ctcaaggcag attgctgaaa
     2881 tctatgcgaa aggacaactg ttaatcgatg ttgatcccat ctttgaaagc ttgtttcttg
     2941 agctgtggga aaaggtgcgc acaatcctgc agaagggtaa gccatgaagc aaattgcgat
     3001 tttaagcgga aaaggcggca gcggcaaaac ctccatctgc gccgcactca tcgatctgat
     3061 ctctcaaaat cattccctta gcatggtgga tgcggatgtg gacgcagcca acctgaatct
     3121 gctgatcccc tgcacagaac aaaccgaaca atcctttcaa ggagggaaaa aagcgattat
     3181 cgctcaggaa agctgcattc gatgcggcat ctgtctgaaa tactgccgtt ttcatgcggt
     3241 tcatagaacg ctggacgatt ctgggctgaa tcctcgcttt tcgatcgatc ttttggcatg
     3301 cgaaggatgc gcagtttgcg cgcagcagtg tccttcttcc gccatttcaa tgcatcctac
     3361 acaagatgga gtctggtatc gatcgtcgag cgaatatggt gagtgttttc acgcccatct
     3421 ctttgccggg catgagaact ccggcaagct ggtttcgacc atcatctccg ccgcacgccg
     3481 gtacgttcaa aattctgcag tcgatctcat cctggtggat ggtccgcccg gaattggctg
     3541 cccggtcatc gcggtctgca atcaaagcga cttggcattg atcgtgacgg aacctacccc
     3601 atccggcatt cacgatatgc aacgcatact ggatacaatc cgctatttcg acctgcccgc
     3661 ctttcttatt attaataaat ttgacctgca tcttcaaaac acggagcaaa ttttggagca
     3721 gtgcgaaaga gaagacgtgc ctgttttagg gcaaattccc ttcgatccac aatttaatga
     3781 agcgattttg catgcccagc ccatcacacg ctacgctacc gattcccttt ctaaccaggc
     3841 tattcacgcc atctggcagc agctaaaaag tcaagttctc aatagttgaa tagatgatac
     3901 aagattcgtt ttacttcttt aatgtacccc accag
//
LOCUS       DAOSDS010000162         3907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_8193_length_3907_cov_4.928089, whole genome shotgun sequence.
ACCESSION   DAOSDS010000162 DAOSDS010000000
VERSION     DAOSDS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8193_length_3907_cov_4.928089"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="PLL88_11620"
     CDS             <1..727
                     /locus_tag="PLL88_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOJ02254.1"
                     /translation="REKGYEPNAMLAFENNEIGKQRNVAKDDLVINACLRIWLEKNGK
                     NTEKIADESIRTAAQKLMEETHIPLPTLGKQILRDGNSGLPYDQPVTVGVMTIMKLHH
                     LVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTIKS
                     DDVQGRVTSYEAIVKGEAIEEPSLPAGFRVLVKELQSLGLAVEAELDSGDVIKFGKDE
                     EKIKPPKLPTGLMGLGEKFEDLS"
     gene            808..1224
                     /gene="rpsL"
                     /locus_tag="PLL88_11625"
     CDS             808..1224
                     /gene="rpsL"
                     /locus_tag="PLL88_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOJ02255.1"
                     /translation="MPTINQLIRRGREQKKSKSKAPALLYTMNHLKQRRTRQGVGSPQ
                     KRGVCTVVRTMTPKKPNSALRKIARVRLTNGLEVTAYIPGEGHQLQEHSVVMVRGGRV
                     KDLPGVRYHVVRGALDTTGVAVRNQGRSKYGTKRAK"
     gene            1254..1721
                     /gene="rpsG"
                     /locus_tag="PLL88_11630"
     CDS             1254..1721
                     /gene="rpsG"
                     /locus_tag="PLL88_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOJ02256.1"
                     /translation="MRRTKPEKRRIDPDIRYNSRNVQVLINNILKKGKKSTASQIVYD
                     ALGIVQETTKKEPVEVLDAALKNVGPVMEVRPRRVGGATYQVPMEVEPERKLTLAVRW
                     ILDASNARSGKSFAEKLAGEVMDAANEQGSAIRKREETHKMAEANRAFSHFRV"
     gene            1765..3834
                     /gene="fusA"
                     /locus_tag="PLL88_11635"
     CDS             1765..3834
                     /gene="fusA"
                     /locus_tag="PLL88_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ02257.1"
                     /translation="MAREYPLDKLRNIGVIAHIDAGKTTTTERILYYTGKTYRIGSVD
                     DGTTVTDWMKQERERGITIVSAAITTRWRDYQINLIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDGVQGVEPQSETVWRQADRFSVPRICFVNKMDRVGADLEYSIQTIKDRLGANPI
                     QMQVAIGKEADFKGAINLLEMKAVIWNDELGSKPEEQEIPDNLRELAEGKRKELLEKV
                     VELNDELIEKFLNEQEITIEEIKRTLRKAVLANKATPVFCGSSLRNRGVQPLLDAVID
                     YLPSPLDIPSVKGTDPRNGDEIIRKTNDEEPMSALIFKIVSDPYAGRMAYFRVYSGTI
                     KQGLMAYNPTTGKKERVGRLIRVYADHREDVEEMHAGDIGAVLGFKSIVTGDTICEAH
                     HPIVLERISFPEPVISVAIEPKTTADNEKMAKALQRLAEEDPTFRVHEDENTGQTIIE
                     GMGELHLEIIIDRMFREFNVQANVGKPRVSYRETITKELPKVEYKYVKQTGGHGQYGH
                     VVFSFKPLPQGSGVVFVNEIKGGTVPRDYFNAVEKGVHEAAESGVLAGYPMTDLQVTF
                     YDGSYHEVDSSEMAFKMAAIFAFREGVEKAHPVLLEPIMKVEVNIPEEYLGDVLGQLN
                     SRRAEVAGIEMKHGNAQSVRAFVPLAEMSGYATELRSVTQGRGVFSMEYDHYTPLPES
                     KASEILH"
BASE COUNT         1137 a          806 c         1044 g          920 t
ORIGIN      
        1 gcgtgaaaag ggatacgagc cgaatgctat gctggcattt gaaaacaatg aaatcggaaa
       61 gcagcgaaat gttgccaaag acgatctggt aattaatgct tgcttgcgga tctggctgga
      121 gaagaacggg aaaaacaccg aaaagatcgc tgacgagtcc attcgaactg cagcgcaaaa
      181 actcatggaa gagactcata ttccgctacc cactttgggt aaacaaatat tgcgcgatgg
      241 gaatagcggt ttgccgtatg atcagccggt taccgtgggt gtcatgacca ttatgaaatt
      301 acatcacctg gtagaagaca aggtgcatgc acgttctaca ggtccttaca gcctagttac
      361 ccaacagccg ctgggtggca aggcgcaatt cggtggtcag cgttttggtg agatggaagt
      421 ttgggctctt gaggcatatg gcgcagcgta tacgttgcag gaaatgttga ccattaagtc
      481 ggatgacgtt caaggaaggg tgacttccta tgaagctatc gtgaagggcg aggcgatcga
      541 agaacccagt ttgcccgccg gattcagggt cttggtaaaa gagttgcaga gcctgggatt
      601 ggcggttgaa gcagaattgg attcaggcga tgtcattaaa tttggaaagg acgaagaaaa
      661 gataaaacca cccaaattac caaccggtct gatgggttta ggagaaaagt ttgaggatct
      721 ctcttgagta ttgtattgac gcaaaaaaaa cggtgtgata tactcgcact ttggttttat
      781 caagaatgaa aatttatcgg aggcaatgtg ccaacaatta atcaactgat tcgaagagga
      841 cgagaacaga aaaaatcaaa gagcaaagcg ccagctttac tctatacaat gaaccacttg
      901 aaacaacgcc ggacccgcca gggtgtagga tctccacaaa agcgtggtgt atgcactgtc
      961 gttcgcacca tgactcctaa gaaaccaaac tcagcgttgc gcaagattgc gcgtgtgcgt
     1021 ttgacaaatg ggcttgaggt cactgcttac attcctggtg aagggcatca gcttcaggaa
     1081 cactccgtgg tgatggttcg tggtggtcgt gtgaaagacc tgcctggtgt gcggtatcat
     1141 gttgtgcgcg gcgcactgga tacaaccggc gtcgctgttc gaaatcaggg gcgctcaaaa
     1201 tacggtacaa agcgagcaaa atagttcgaa tcatgacctg tggagaaagt tgaatgcgaa
     1261 gaacaaaacc agaaaaacga cgaattgatc ctgacatccg ctataacagc cgtaatgtgc
     1321 aggttctcat caataatatc ttgaaaaaag gcaaaaagag caccgcatcg cagatcgttt
     1381 atgatgcctt gggaattgtt caggagacaa ccaaaaagga gccggttgag gtgttggatg
     1441 cggctctcaa aaatgttgga ccggtgatgg aagttcgacc gcgccgtgtg ggtggcgcaa
     1501 cttaccaggt tccgatggaa gtagaaccgg aacggaaatt gaccctggct gttcgatgga
     1561 tcttggatgc gtctaatgcg cggagcggaa aaagctttgc agaaaagttg gctggcgaag
     1621 tgatggatgc agccaatgaa caaggctctg cgataagaaa acgcgaagaa actcacaaaa
     1681 tggcggaggc aaaccgggct ttttcacatt tccgggttta attccttact ttcttaatca
     1741 tttgaacttt aacaggaaaa caatatggcg cgagaatatc ctttagacaa actacgtaac
     1801 attggggtta ttgcgcatat tgatgcagga aaaaccacca ccactgagcg gattctatat
     1861 tacactggca aaacgtatcg aattggctca gtggatgatg gcactaccgt tactgattgg
     1921 atgaaacagg aacgggaacg ggggatcaca attgtatccg ctgcgattac aacgcgctgg
     1981 cgggattacc aaattaattt aattgacaca ccaggacaca tcgattttac agccgaagta
     2041 caacgctcat tgcgcgtatt ggacggtggc gtggttgtct ttgatggtgt tcagggcgtc
     2101 gagccgcaaa gcgagaccgt ctggcggcag gcagatcggt tcagcgttcc tcgtatttgc
     2161 tttgtgaata agatggaccg cgttggcgct gacctggagt attccatcca gaccatcaaa
     2221 gatcgcctgg gtgcaaatcc gatccagatg caggtcgcga ttggaaaaga agccgatttc
     2281 aaaggtgcca tcaatctttt ggagatgaag gcagttatct ggaatgatga acttggatcg
     2341 aaaccagaag aacaagagat ccctgacaat ttgcgagaac ttgcagaagg aaaacgcaaa
     2401 gaacttcttg aaaaagtcgt cgaattgaac gatgaactga ttgaaaaatt cctcaacgag
     2461 caagagatca cgattgaaga gattaaacgt accttacgga aggctgtttt agcaaataag
     2521 gcaacgccgg tgttctgtgg ttcatccctg agaaataggg gagtgcaacc cttgctagac
     2581 gccgtcattg attacttacc ctcaccgctc gacattccat ctgttaaagg aactgacccg
     2641 cgcaatggag acgagatcat ccgaaaaacc aatgatgagg aacccatgag cgcgctgatc
     2701 ttcaaaattg tgtccgatcc ctatgccgga cgcatggctt acttccgcgt ttattccggc
     2761 acgatcaaac agggcttgat ggcttacaat ccgaccactg gaaaaaaaga gcgcgtcggt
     2821 cgtttgatcc gcgtttacgc ggatcaccgt gaggacgtgg aagaaatgca tgccggcgat
     2881 atcggcgcag tgcttggttt caagagcatt gtcacgggcg atactatctg cgaagcgcat
     2941 catcccattg ttctggaaag aatttcattc ccggagcctg ttatttctgt ggcgatcgaa
     3001 ccaaaaacaa cggctgataa tgaaaaaatg gctaaagctt tgcagcgttt agccgaagag
     3061 gacccgacat tccgcgttca tgaagatgaa aataccggtc agaccatcat tgaaggaatg
     3121 ggtgagcttc atctggagat catcatcgat cggatgttcc gcgaattcaa tgtacaggca
     3181 aacgttggaa agccgcgagt ttcctatcgt gaaacgatta ccaaagaact gcccaaagta
     3241 gaatataaat atgtgaagca aaccggtggt catggtcaat atggacatgt ggttttttcg
     3301 ttcaaaccat tgccccaggg tagtggggtg gtgtttgtca acgaaataaa aggcggcacc
     3361 gttccgcgcg attatttcaa cgctgtggaa aaaggcgtgc atgaggctgc tgaatctggt
     3421 gtgttagcgg gctaccccat gaccgatctg caggttactt tctatgacgg ttcctatcat
     3481 gaagtcgatt cttcagaaat ggctttcaaa atggctgcta tctttgcctt ccgggaaggt
     3541 gtcgaaaagg cacatccggt tttgcttgag cccattatga aagttgaagt gaatatccct
     3601 gaggaatatc tgggtgatgt tttggggcag ttgaattcaa ggcgtgctga ggttgcggga
     3661 attgaaatga agcatgggaa tgcccaatcg gtgcgcgcat ttgttccttt ggctgaaatg
     3721 agcgggtatg cgacagaact gcgttctgtt actcaggggc gaggtgtgtt ctcgatggaa
     3781 tacgaccatt acacaccctt acctgaaagt aaagcaagcg aaatcttgca ttaagtaata
     3841 taaaaatgta aaaggagaaa gaaaatggca aaagaggtat atgatcgaac gaagccacat
     3901 ttcaacg
//
LOCUS       DAOSDS010000163         3778 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_8555_length_3778_cov_3.833736, whole genome shotgun sequence.
ACCESSION   DAOSDS010000163 DAOSDS010000000
VERSION     DAOSDS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8555_length_3778_cov_3.833736"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="PLL88_11640"
     CDS             complement(<1..1084)
                     /locus_tag="PLL88_11640"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ02258.1"
                     /translation="MNKKKNLFGSWKNWRIKTLYILILLVFGFYGFRLFTLQIIDGEE
                     YIAQADENRISNVSIQTERGIIYDRNGIVLAKNAAAYNVMITPADLPTDEGATQEIYR
                     QLSEVIDIPVSNGELTDEVVRMFSPCNTDLGIAEIVEIADTNAPYTPMEIKCDVDKMT
                     AMLIESQSDEWPGITIEVEPIREYPTGELTAEVVGFLGPIPESQEEYYTELGFVAGRD
                     KVGYSGVEATMQDILAGKNGLRVVEVDVAGKVIRDVEPPVESEAGLNVKLTIDTRLQQ
                     AAKAALIGNIDWWNRWFNDVRFTNGVVIAMNPKTGEILALVSYPTYENNRMARFIPAY
                     YYQQLSEDPNRPLFNHAISAEHPPGSV"
     gene            complement(1077..1577)
                     /locus_tag="PLL88_11645"
     CDS             complement(1077..1577)
                     /locus_tag="PLL88_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02259.1"
                     /translation="MDILLSSIFVLIFASLEVGVISRIPLENGMADIMLLFVITWSLN
                     PQAKRYYIPALLAAGVMTFISSVPIPGILMSYALAAGLTRLLVNRLWEMPVFSMLIVT
                     IVATMLQHVIYILIMQFQGSAVPFLTSLREITLPSIFLNIIFSFPVFLLVRDAQKAVF
                     KEVENE"
     gene            complement(1588..2424)
                     /gene="mreC"
                     /locus_tag="PLL88_11650"
     CDS             complement(1588..2424)
                     /gene="mreC"
                     /locus_tag="PLL88_11650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOJ02260.1"
                     /translation="MRFKNTRAVRTTVTALVILGIVLLAFSGLLRPLLGAVMDPFVKV
                     QNWISTQFMAIYDFLTLPREVTDLMAENAQLKNEVSQLQSQIIGLQEQLREADILYAL
                     LDFARAKPEYSYVAASVIGRDPNPFMHYLIIDHGSDDGIRYGMPVVTQQGLVGKVDAV
                     TASAARIQLITDPDSSINVRMQSLDEEAQLMGSITGDLQLTMISADIALQPGEILLTS
                     GLGGNYPADIVVGQVSSVQKKESDIFQTAFVQSAIDYSTLRAVLVIVNFTAIDINLLT
                     GE"
     gene            complement(2471..3535)
                     /locus_tag="PLL88_11655"
     CDS             complement(2471..3535)
                     /locus_tag="PLL88_11655"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ02261.1"
                     /translation="MVANPFNWLLGLFSLDLGIDMGTANTLVYVRGKGIIVHEPSWVA
                     IEKKSRRPVAIGTQAKQMVGRTPNNITTIRPIRDGVISEFEITQAMLAYFIGRVHQQA
                     IVPMPRPRVVIGIPTGATEVEKRAVFDATMAAGAREVYLIDEPVAASIGAGLPLADVS
                     GSMIVDIGGGTTEIAINSMGGIVVSRSLRVAGDEMDQAIIEYIRNKYSMFIGERMAEQ
                     IKWEIGSVFPFESEKTMQIKGRNLITGLPESIEISSIEVRDALSNSIQVIIDTIKNAL
                     DEVPPEIGSDLLDTGICLAGGGSLLNGMVLRLSEELKMHVWLAEDPMTCVARGAGIVL
                     EDLERFKHFLVDIDGNKQHM"
     gene            3707..3778
                     /locus_tag="PLL88_11660"
     tRNA            3707..3778
                     /locus_tag="PLL88_11660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3739..3741,aa:Gly,seq:ccc)
BASE COUNT         1011 a          991 c          824 g          952 t
ORIGIN      
        1 ataccgagcc cggggggtgt tcggcagaaa tagcgtggtt aaaaagcggt cggttaggat
       61 cttcactaag ttgttgataa taataagctg ggatgaaccg cgccatgcga ttgttctcat
      121 aggttggata agaaactaac gcaagaattt ctccggtttt ggggttcata gcgatcacga
      181 ccccattagt aaaacgcacg tcattgaacc agcggttcca ccaatcgata tttcctatta
      241 aagccgcctt tgccgcctgt tgaagccttg tatcgatggt cagtttcacg ttcaatccgg
      301 cttcgctctc aaccggtggc tctacatcgc gaatcacttt acccgccacg tccacttcca
      361 ccacgcgcag accattcttg cccgccagga tatcttgcat ggtggcttcg acgcccgagt
      421 agcctacttt atcacgcccc gccacgaaac cgagctctgt ataatattct tcctgcgatt
      481 ccggaatggg tcccagaaaa ccgaccactt cagccgtcag ttcccccgtg ggatattctc
      541 tgatcggttc gacctctatg gtgatccccg gccattcatc cgactgcgat tcaatcagca
      601 tggcagtcat tttatcaaca tcacatttta tctccatggg tgtataagga gcattggtgt
      661 ctgcgatctc tacgatttcg gcaattccca gatcggtatt gcaaggggag aacattctca
      721 caacttcgtc cgtcagctcg ccattcgaaa ccggaatgtc gattacttcg gaaagctgcc
      781 ggtatatctc ctgtgtggct ccctcgtcgg ttggcagatc agccggcgtg atcatcacat
      841 tatacgcagc ggcatttttt gcaagtacga taccgttgcg atcatagatg atcccacgct
      901 cggtttgaat gcttacattg ctgatgcggt tctcatctgc ctgagcgatg tattcttcgc
      961 catcgatgat ttgcaaagtg aacaggcgaa atccataaaa cccaaagacc agcaagatca
     1021 ggatatatag cgttttgata cgccaattct tccaggaacc aaacaggttc tttttcttat
     1081 tcattttcca cttccttaaa caccgccttt tgagcatcgc gcaccaacaa aaatacgggg
     1141 aaagaaaaga tgatgtttaa aaagatactg ggtaatgtga tttctcgcag acttgtaaga
     1201 aaaggaacag cagaaccttg aaattgcatg atcaagatat agatcacatg ctgcagcatt
     1261 gtcgcaacaa tggtcacgat caacatggaa aaaacgggca tttcccataa acggttcacc
     1321 aaaagtctgg tcaaccctgc cgccagagca taactcatga gaattcccgg aattggaact
     1381 gatgagatga aggtcatcac gccagccgcg agcaaagccg gaatgtagta ccgttttgcc
     1441 tgcggattta agctccaggt aatgacgaac agcaacatga tatctgccat cccgttttcc
     1501 agtggaattc ggcttatcac tcccacttcc agcgaggcaa agatcaatac aaatatggaa
     1561 gaaagcaaaa tatccatttt ttgtgcccta ctcacccgtt agaaggttga tatcgatcgc
     1621 agtgaaattg acgatcacca ataccgcgcg caaggtggaa tagtcaattg ctgattggac
     1681 aaaagcggtt tggaaaatat cgctttcttt tttctgaact gaagatacct gaccaaccac
     1741 aatatctgcc ggataattac cacccagacc tgaggtcagc aagatctccc ccggttggag
     1801 agcaatatct gcggatatca tcgttaactg gaggtctccg gtaatcgaac ccattaattg
     1861 ggcttcctca tccagtgatt gcatgcgaac gttaatgctg gaatccggat cggtgatcag
     1921 ttggatgcgg gcggcgctcg cagtaacagc atccacttta ccaaccaagc cttgctgggt
     1981 aacgaccggc attccatatc ggatcccatc gtcagaacca tgatcaatga ttagatagtg
     2041 catgaacggg ttggggtcac gaccgatcac agaagctgcc acgtaagaat attcaggttt
     2101 cgcgcgcgcg aaatcgagca gcgcatataa gatatccgct tctcggagtt gctcttgcaa
     2161 tccaatgatt tggctctgta attgtgaaac ttcgttcttt aattgtgcat tttctgccat
     2221 gaggtcagtt acctcacgcg gcagggtaag aaaatcatag atcgccataa attgggtaga
     2281 tatccaattc tgcactttca caaacggatc catcaccgct cccagcaaag gacgcagcaa
     2341 gccgctgaaa gccagcaaga cgatccccaa gataaccaac gcagtgactg tagttcgaac
     2401 cgcacgcgta tttttgaaac gcattcttca ccaatctcgt tttttcaata atacctggag
     2461 aatcacataa ttacatatgt tgtttgtttc catcaatatc aacgaggaaa tgcttgaagc
     2521 gctcgagatc ttcaagcaca ataccggcac cacgcgcaac acaggtcatg ggatcttcag
     2581 ccaaccatac atgcattttc aattcttcag acaatcgcag aaccatgccg ttcaacaacg
     2641 atcctccgcc tgccaggcaa atgcccgtat ccagcagatc agaaccaatt tcaggaggta
     2701 cttcatccaa tgcattcttt atcgtatcaa tgatcacctg gatcgaattg ctcaaagcat
     2761 cacgaacctc aatagaagag atttcaatcg attcaggaag ccccgtgatc aggttgcgcc
     2821 cctttatctg catggtcttc tccgattcga aggggaaaac ggaaccgatt tcccatttga
     2881 tttgttcagc catacgttcc ccgatgaaca tgctgtattt attgcgtatg tattcgatga
     2941 tggcttgatc catttcatct cctgccaccc gcaaagatcg ggaaacgaca atccctccca
     3001 tcgaattgat cgcgatttcc gtggttccac ccccaatgtc cacgatcatg ctccccgaca
     3061 catctgccag cgggagtccg gctccaatac tggctgcaac cggttcatcg atcaaataaa
     3121 cctcccgtgc acctgcagcc atggtggcat cgaagacagc ccgtttctcc acctcggttg
     3181 caccggtcgg gattccaatg acaacacgcg gtcgcggcat gggcacgatc gcctgctgat
     3241 gcacacgccc gatgaagtaa gccagcattg cctgggtaat ctcaaattca gaaatgacgc
     3301 catcgcgaat ggggcgaatg gttgtaatgt tgtttggggt tctcccaacc atctgtttgg
     3361 cttgtgtgcc aatggcaacc gggcgccgtg actttttttc aatggctacc caggatggct
     3421 catgaacaat gatgcctttt cctcgcacat agaccagtgt attggctgtc cccatatcaa
     3481 ttcccaaatc gagagagaat aatcccaata gccagttaaa tggatttgca accaaacgaa
     3541 actcctgtgt aaaaactgtc gaattctcta ccacaaatcg ggcaatatta taacatgata
     3601 ccattaattc acatccgtcc cgggagcgat tggaaaacca tccatccaga ggatcaaaca
     3661 ccaacctatt gacacccttg cagcgcctcg atataatgca actgttgcgg gtgtagttca
     3721 atggtagaac gtctgcttcc caagcagaag gttgtgggtt cgagccccat cgcccgct
//
LOCUS       DAOSDS010000164         3588 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_9138_length_3588_cov_6.240023, whole genome shotgun sequence.
ACCESSION   DAOSDS010000164 DAOSDS010000000
VERSION     DAOSDS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9138_length_3588_cov_6.240023"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PLL88_11665"
     CDS             complement(<1..821)
                     /locus_tag="PLL88_11665"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HOJ02262.1"
                     /translation="MQIFQSNAEFKSCHAVALCEMTNGELLAAWFAGDEEGAKNSVIL
                     TSRKEKNKEEWSPARVSVNVIDKAAGNPRLFIGPDRAVWLLAPINYGKWCQGGTRLFV
                     KRSYDGGRSWSDLELFLDQAGILGKNKPICTKAGTWILPCEYEESWQAVFLRLPKGSE
                     SWEIINCPKTNARLHQPSIVQKENGELLAFMRTWEGVIYQARSNDDGNTWSEPEATGI
                     PNNNSGIDMVKLTKNSIVLVCNPCGLGRDGSIVVYPEDRPAEINVQELCEADSKQ"
     gene            complement(830..1567)
                     /gene="kdsB"
                     /locus_tag="PLL88_11670"
     CDS             complement(830..1567)
                     /gene="kdsB"
                     /locus_tag="PLL88_11670"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOJ02263.1"
                     /translation="MFVLGIIPARYGSSRLEGKPLKDICGKPMIQHVYERVQLSTLIN
                     EVVVATDDERIVAVVKNFGGKAQMTSINHKNGTDRIAEVAKAIEADVVVNIQGDEPLI
                     DPRMIDEAIQPMLDDRELKACTLCRPILSEEDISSPHVVKTVFDLKGNALLFSRAPIP
                     YPRNKEAYTAYEHIGVYVYRKDFLMQYVNMPQTPLEITESLEQLRILENGIRMKVVKV
                     TVPYEGLSVDTQEDLERVREIIKKRAQ"
     gene            complement(1577..2788)
                     /locus_tag="PLL88_11675"
     CDS             complement(1577..2788)
                     /locus_tag="PLL88_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02264.1"
                     /translation="MPLFGMTDAKIESYEAQLSPETRNFVQLSIEKIVKAKETGKKVV
                     VILGSGPNLHEGVTTLMAELMDKGLVDGLSTSSAVISHELAGVLDKVKRVNGVKLGIN
                     KNLLPKGEFFEITQLPPTWFDVIDQEMNIDHELMQRGNQLEGDVIIKAAGNMGYPMGL
                     RSERIAKEILGIAKAYGVPFEAAAGWGADPYTMIGSGARKELPVLVSVPQLIGGGGVG
                     LCIGDSISITERCMKMAQMLGSADVLIESAVALTQEIHDGPFETYTGHGVWGYWDGYK
                     SYSLENKTLIRIDLDPNLKRAWDMQRQDSSVQNAINKGLPKTKLTGIPFRMEMSGFAR
                     LETSIPVVGDIGVVWPVIAWHVAKRLKIELDFINHPQQTEEGQRMREWIVDEIRPFDR
                     EQMIKIGQSRQ"
     gene            complement(2815..>3588)
                     /locus_tag="PLL88_11680"
     CDS             complement(2815..>3588)
                     /locus_tag="PLL88_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOJ02265.1"
                     /translation="LVPILVMVSTSLKPQGQVLSRTWEWIPRTFTVENYQYVLQQYPY
                     LKWTTNSLIVTLGTVVLTLIVSLPAGYAFARLKMRGKNILFSMVMLTIMIPFFAYTPQ
                     LYLLITKMGLKNTYWGLILPMSTSGVSVFLFKQFIQQLPLDLEEAAVLDGCSNWGVFF
                     KVILPLTKPAIVTSVIFTAVKSWNNLLWPLIASSNDAVKTLPVGLSVNVFQVETGLAN
                     QPPYGIVMAAALLSIILPVLLFIFLQRYFVQGIATTGLK"
BASE COUNT          897 a          949 c          730 g         1012 t
ORIGIN      
        1 atttgtttcg aatccgcctc acataattcc tgcacattga tttcagcagg tcggtcttct
       61 ggatatacga ctatactgcc atcacgccca agaccacaag gattgcagac caaaactatg
      121 ctatttttgg ttaatttaac catatcaatg ccactgttat tgttgggtat tccagtggct
      181 tcaggctccg accacgtatt cccatcgtca ttggatcgag cctggtaaat cacaccttcc
      241 cacgttcgca taaaagccag caattctccg ttctcttttt ggacaatgct tggttggtgc
      301 aagcgagcgt tcgttttggg gcagttgata atctcccaac tttcgctccc ttttggtaga
      361 cgcaggaaaa ccgcctgcca actttcttca tattcgcagg gcagtatcca ggtacccgct
      421 ttggtacaaa tgggtttatt ttttccgaga attcccgcct ggtcgagaaa aagttcaaga
      481 tctgaccatg acctgccacc atcgtaggaa cgttttacga acaacctcgt tcccccttga
      541 caccacttgc cataattaat gggggcaaga agccacaccg ccctatccgg tccgatgaat
      601 aatcgcggat tgccagccgc tttatcgatc acgttcacgc tgacgcgcgc tggcgaccat
      661 tcttccttgt ttttctcttt gcggctggtg agtataacgg aattctttgc gccctcctca
      721 tctcctgcga accatgcggc aagcagctct ccattcgtca tctcgcataa ggctacagcg
      781 tgacaggatt tgaactcggc attgctttga aagatttgca tagaccccat cattgagccc
      841 gctttttaat aatctctcgt acacgctcca ggtcttcttg cgtatctacg ctgagacctt
      901 catagggaac tgttactttc acaaccttca tccgtatccc attctcaaga atgcgcaatt
      961 gttccaaaga ttcggttatc tctaacgggg tttgcggcat gttgacgtat tgcatcaaga
     1021 aatcttttct gtaaacgtac acaccaatat gttcataggc tgtatacgct tctttattgc
     1081 gaggataggg gattggtgct ctggaaaaaa gcagtgcgtt cccctttaaa tcaaataccg
     1141 tctttaccac atgggggcta gagatatctt cctccgaaag gatcggacgg cagagagtgc
     1201 acgctttcaa ttcccgatca tccaacatgg gttgaatcgc ttcatcgatc attcgcgggt
     1261 cgatgagcgg ttcgtcgcct tggatattca caacgacatc cgcttctatc gcttttgcaa
     1321 cctcagcgat gcgatctgtt ccattcttgt gatttatgga agtcatttga gcttttccgc
     1381 caaaattttt taccaccgct acaattcttt catcatcggt agcaacaacg acttcattga
     1441 tcaatgttga taactgaaca cgttcataaa cgtgttgaat catcggtttt ccgcagatat
     1501 cctttaaggg tttcccttca aggcgtgatg acccatagcg agcggggatg atacctaaaa
     1561 caaacatttt tctctcctac tggcgtgatt gcccgatctt tatcatttgt tcacggtcga
     1621 acggacgaat ttcatccacg atccactcgc gcattctttg tccttcttcc gtctgctgcg
     1681 gatggttgat aaaatccaat tcgatcttta acctttttgc gacatgccag gcaataacgg
     1741 gccaaacaac gccaatatcc cccaccaccg gaatgctggt ttccaacctg gcgaagcccg
     1801 acatttccat gcggaatgga attcccgtca gttttgtttt tggcaagcct ttgttaatcg
     1861 cattttgcac ggatgaatcc tgcctttgca tatcccacgc gcgcttgagg ttagggtcaa
     1921 ggtcaatgcg gataagcgtt ttattttcca aactgtagct tttatagcca tcccaatatc
     1981 cccaaactcc atgaccggta taggtttcaa aaggaccatc gtgaatttcc tgggtgagtg
     2041 ccacagccga ttcgatcaac acatccgcac ttccaagcat ctgtgccatt ttcatgcatc
     2101 gctccgtgat tgaaatgctg tctccaatgc ataagccaac cccacccccg ccaatcaatt
     2161 gaggaacgct aaccagcaca ggcagttcct ttctggctcc gcttcctatc atggtatagg
     2221 ggtcagcacc ccaaccagcc gccgcctcaa aaggtacgcc atatgccttg gcaatgccca
     2281 gaatctcttt cgcaattctt tcagagcgca gccccatggg gtaacccatg tttccggctg
     2341 ccttgataat cacatccccc tccagttggt tgccacgctg catcagttca tggtcaatgt
     2401 tcatctcttg gtcaataaca tcgaaccagg ttgggggtag ttgagtgatc tcaaaaaact
     2461 cacctttcgg taacagattc ttgtttattc ccagtttgac accattgact cgtttgacct
     2521 tatccaacac gcccgcaagt tcgtgcgaaa tgaccgctga actggtcgat agcccatcca
     2581 ccagcccttt gtccatcaac tcagccatca aggtggtcac gccttcgtgc aaatttggtc
     2641 cgctgccaag tatgacaacg actttcttcc ccgtttcctt tgctttgaca attttttcga
     2701 ttgaaagctg aacgaagttc ctggtctctg gactcaattg agcttcataa ctctcaatct
     2761 ttgcatcagt cattccaaat aacggcattt ccttttctcc taaaatgatc ctttttattt
     2821 cagtccggtg gtcgcgatcc cttgaacgaa gtagcgttgt aagaagataa acagaaggac
     2881 aggcaaaata attgatagca aagctgccgc cataacaatg ccatatggcg gttggttagc
     2941 aagccccgtt tctacctgaa agacattcac cgacagcccc actggcagtg tcttcacggc
     3001 atcgttactg ctcgcaatga gcggccacaa caagttattc caacttttaa ctgcggtgaa
     3061 gatcaccgag gtgacaatag cgggttttgt caatggcaga atcactttga aaaaaacgcc
     3121 ccagtttgaa catccatcca aaacagcagc ctcctccaga tcgagtggca attgttggat
     3181 gaactgtttg aacaggaaaa cacttacccc gctggtactc atgggtaaaa tcaatcccca
     3241 ataggtattc ttcaatccca ttttggttat taacaaatag agttgcggag tatatgcgaa
     3301 gaaaggaatc ataatagtca gcatcaccat agaaaaaagt atgttcttcc cgcgcatttt
     3361 taaacgggca aaagcatacc ctgccggcaa gctcactatt aatgtcaata caacagtgcc
     3421 aagcgtgaca atcaagctgt tcgttgtcca ttttagatat ggatactgtt gcaacacata
     3481 ttgataattc tccacggtaa acgttcgcgg aatccactcc catgttctgg aaagcacctg
     3541 cccttgtggt ttcagcgacg ttgataccat aacgaggatt ggaacgag
//
LOCUS       DAOSDS010000165         3578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_9176_length_3578_cov_4.368436, whole genome shotgun sequence.
ACCESSION   DAOSDS010000165 DAOSDS010000000
VERSION     DAOSDS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9176_length_3578_cov_4.368436"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /gene="radC"
                     /locus_tag="PLL88_11685"
     CDS             <1..622
                     /gene="radC"
                     /locus_tag="PLL88_11685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOJ02266.1"
                     /translation="ELLAILLRVGVEGESAVALGTRLLDELQGIAGIHAAELEELCNV
                     HGIGLAKAAQIKAALELGSRLHQKTLEERPVIDSPERAYEVLKFDILENSRETLWILA
                     LNTRHRLLKKKKLYQGTRSFSSVRVAEIFEFALLQHAAAIILAHNHPSGEPYPSQEDL
                     RITQEIRKAGEILEIPLLDHLIIVPGAFQSLKKEHKGIFDSSNYAS"
     gene            complement(685..1560)
                     /locus_tag="PLL88_11690"
     CDS             complement(685..1560)
                     /locus_tag="PLL88_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02267.1"
                     /translation="MSRKHIVILSLIFVLCSCTTTPSQKAVQTAIAQTANAEQDIPDS
                     MIQTAIAKTQTASEPAATAQPETDQDVAVSCELSTPYHADWAVSFCDTFSDNRNGWEL
                     GSGSDDLSRSIYSIEDGKLVVDLTGKATSGYLSGVIQWLPVIASEDFVITLKGDVFSD
                     YKSCSWGIVFNEWDNANFYAFMISSRVGTYSLTKFEDGDQDFPIPGKSHGAITWDDEN
                     ELTIVAENGYYQFFINGTFVDDYETRNIKGTTIALATWTGEGVTARFEFDDLLIKTPE
                     SKSSNTNNLITYFIQ"
     regulatory      1718..1801
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1854..3479
                     /locus_tag="PLL88_11695"
     CDS             1854..3479
                     /locus_tag="PLL88_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOJ02268.1"
                     /translation="MEKLLLLYLRDKRSARCVYFSIFILQIIIAYCFYLNPHYDANFT
                     HLMYVAITLSACFFPICVAIGQSITAGVILGFVYSLTNVERGGNLTAYLLLTIGYFVS
                     ATLVIGFLKNKIINFYLRKEDIFYIDEFTGLPNMSAFFRDVQQYQDQHDLQPRQFILV
                     EVINQKEISAAFGMKFYYKLEKEMAHTARDFFQMDIQAYQIRLGTLAILFPPNNPVDL
                     SRMKERPIQTVTVDHIPVFFDIVYGCCEFPRDGTSGDELLQKGFLALGEAHQRNQLYY
                     AYNPTLQVPQKVWLMGQIQNAIDDNEIIFHYQPILGVHGEVHDIEALVRWDHPRLGLL
                     PPSDFIPDLELTEISDNLVDYSLKYNLHSLKKLISQGLDLHMAINVTIRNLHQINFAR
                     NVLALLEEFQLRPSNLVLEITERGFLADDEESNHNIQELSKNGVSFHIDDFGVGFTSL
                     GNLRKFGIRSIKIDQSFITNLQKDPIDRGLVKSVIAMARTVGISTVAEGVESREMLTI
                     LQKMGVDYFQGYAIANPMPYDELCRWLKEHHKH"
BASE COUNT          980 a          741 c          843 g         1014 t
ORIGIN      
        1 ggaactgctg gcaatcttgc ttagggtggg tgtggaaggt gaaagcgctg tagcgctggg
       61 aacccggtta ttggatgaac tgcaaggcat cgcgggcatt cacgcagcgg aattagagga
      121 attatgcaac gtgcacggga tcgggctggc taaagcagcc cagatcaagg cagccctgga
      181 attaggatcg cgactgcatc agaaaactct ggaggagcga cctgttattg acagtcctga
      241 gagagcgtat gaagttttga agttcgatat tttggagaat agccgggaga cgctatggat
      301 actggcgctc aacacccgac accgtttatt gaaaaagaaa aagttatatc agggtacgcg
      361 aagtttctcc tcggtcaggg tggcagaaat attcgaattc gcattattgc agcatgcagc
      421 cgctatcatt ttggcgcata atcaccccag cggtgagcca tatccaagcc aggaggatct
      481 gcgcattaca caagagatcc gcaaagcggg agaaatttta gagatcccgt tacttgatca
      541 tctcatcatc gtgccaggcg cctttcaatc cttgaagaaa gagcataagg gaatatttga
      601 ttcgtcgaac tacgcttcgt aggttagata tcgaagttgc agggatatcc tgttttgaat
      661 ggatgacttt tttacgagca ccctttactg gatgaaatac gttatcaagt tgttcgtgtt
      721 tgacgatttt gattcgggtg tttttatcag caggtcatcg aattcgaaac gtgcggttac
      781 tccctcgccc gtccaggttg ccagggcaat ggtggtaccc ttgatattcc gtgtttcata
      841 atcgtctaca aaggttccat ttataaaaaa ctggtaatag ccattctcag cgactatcgt
      901 gagctcattc tcatcatccc aggtgatagc accgtgagat ttgccgggaa ttgggaaatc
      961 ctgatcccca tcttcaaatt tggtaagcga atacgtgcct acacggctgc tgatcatgaa
     1021 tgcatagaaa ttggcattgt cccactcgtt gaaaacaatg ccccacgagc agcttttata
     1081 atcactgaaa acatccccct tcaaagtgat cacaaaatcc tctgacgcga tcactggcag
     1141 ccattgaatg actccactca gataaccaga agtggctttg ccagtcaaat cgactaccaa
     1201 ttttccatct tcgattgaat aaatggaacg agagagatca tccgaaccgc ttccaagttc
     1261 ccatccatta cggttatccg aaaaagtgtc acagaatgaa acagcccaat ctgcatggta
     1321 gggggtagat agctcacaac ttacagcgac atcctgatcc gtttcgggtt gtgcggttgc
     1381 agcaggttcc gatgcggttt gcgtctttgc gatggcggtc tggatcatcg aatcggggat
     1441 atcctgttcc gcgtttgcag tttgtgcaat ggcagtttga actgcttttt gtgatggagt
     1501 agttgtacat gagcaaagaa caaaaatcaa cgaaaggatg acgatgtgtt ttctagacat
     1561 tcattttctc catgcaaatt gttactaaaa gtatatcaag gggcttttta ttaggcaatg
     1621 attgaactac aaagaatctc atctcagtga gatgtagaaa gtggttgaag tttatgctag
     1681 aattatgcaa gaattcatta taaaaaataa caatctatac tgataaaggc aaaacctgtg
     1741 aaaacaggtg acgcaaagcc gcgagcctac gatcttcgat tatggtagtc gggttgccgg
     1801 tagaaaaata ccggacattg ccatccggtt ttttctttaa taaggtggcg atcatggaaa
     1861 aattattact actatacctt cgagataagc gaagcgcccg gtgtgtttat ttttcgattt
     1921 tcatccttca gatcatcatt gcgtattgtt tttatttgaa tccccattat gatgctaatt
     1981 tcacccacct catgtatgtt gcaattactt tatctgcctg cttcttcccc atttgtgttg
     2041 cgatagggca gagtatcacc gctggagtga ttttgggatt tgtatacagc ctcacgaatg
     2101 tcgaaagggg tggaaactta acagcgtatt tgctgctcac aattggttat tttgtcagcg
     2161 caacccttgt cattggattt ttaaagaaca agatcattaa tttctacctc aggaaagaag
     2221 atattttcta cattgatgaa ttcaccggtt taccgaatat gtcggctttt tttcgggatg
     2281 ttcagcagta tcaagaccag catgacctgc aaccaagaca gtttattctg gttgaagtca
     2341 tcaaccagaa agagatcagc gctgcctttg gcatgaagtt ttattacaaa ttggagaagg
     2401 aaatggcgca cactgccagg gactttttcc agatggatat tcaggcatat cagatccgat
     2461 tgggcactct tgcaatcctt tttccaccta ataatccggt cgacttgagc aggatgaaag
     2521 aaagacctat ccaaacggtc accgttgatc atattccggt tttttttgac attgtttatg
     2581 gttgttgtga attccctcgg gatggtacca gcggtgatga attgcttcaa aaaggatttc
     2641 tggctttggg tgaagctcat cagcgcaacc aattatatta tgcttacaat ccaacattgc
     2701 aggtgcctca gaaagtatgg ttgatggggc agatacagaa tgccatcgat gataatgaaa
     2761 tcatttttca ttatcaaccg atcctgggcg ttcatggaga agttcatgac attgaggctc
     2821 tggttcgctg ggatcatcct cgtcttgggc tgctacctcc atccgatttt atcccagacc
     2881 tggagctgac cgagatctcg gacaatctgg tggattattc actgaaatat aatctgcata
     2941 gcttaaaaaa actcatttct caagggcttg atctccacat ggcgatcaac gtaactatcc
     3001 ggaacctgca tcagattaac tttgccagga acgtgctggc tttgctcgaa gaatttcaat
     3061 tgcgaccttc caatcttgtc ctcgaaataa ccgaacgtgg ctttttagcg gatgatgaag
     3121 agagcaacca caatatccag gagctttcaa aaaacggtgt ttcctttcac attgatgatt
     3181 tcggtgtcgg ctttaccagc ttgggcaatc tgcgtaagtt tggtattcgc agcatcaaga
     3241 tcgaccaatc ttttatcacc aatctgcaaa aagatccgat tgatcgaggg ctggtgaaga
     3301 gtgttattgc aatggcaaga acggttggca tttccacagt ggcagaagga gtggaatcaa
     3361 gagaaatgct gactatcttg cagaagatgg gcgtggacta ttttcaaggg tatgccattg
     3421 ccaatcctat gccgtatgat gaattgtgca gatggctgaa agagcatcat aagcattgag
     3481 aagcggattt ctcttgtttg gtttctctga acaagttcag atggatatga gagaggggag
     3541 ctagacagga acggcaatcg gggtcatcct tatgggtg
//
LOCUS       DAOSDS010000166         3568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_9202_length_3568_cov_4.856248, whole genome shotgun sequence.
ACCESSION   DAOSDS010000166 DAOSDS010000000
VERSION     DAOSDS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3568
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9202_length_3568_cov_4.856248"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLL88_11700"
     CDS             <1..561
                     /locus_tag="PLL88_11700"
                     /inference="COORDINATES: protein motif:HMM:NF037628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOJ02269.1"
                     /translation="EQQGGLIRASCRSVEEFNITYALDRCSDLLVQYGGHSMAAGLTV
                     EKENMALLKEKLWQIAQSEVDWEETSAKLQLDAEAAAVNVASNITSILKALELFEPTG
                     EGNRRPLLFSRGVRVVEKRAVGKTAKHLKLKLRDGNVLWDAIAFGFGDKINAIDEFVD
                     VAFHFGVNDFSGGVQLVVRDLRAASR"
     gene            complement(558..1346)
                     /locus_tag="PLL88_11705"
     CDS             complement(558..1346)
                     /locus_tag="PLL88_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ02270.1"
                     /translation="MPETKRGNFSLTNSQNILSQKPGAFPLCTSFIITYMRVSVIIPV
                     YNEESSILTTYQKVKATNLVHEMLFVDDGSSDRTRSILASIHDPSVVIYTHPANRGKG
                     AAVCTGLQHASGDIAIIQDADLEYDPQDYAALLEPIRERSAQVVYGSRFLTCVPEGIL
                     FWNKLANRFLTFATNLLYGTHLTDMETGYKVFHRDVLKQLTLTSDGFEIEPEITARLI
                     KLRIPIYEVPVRFHPRAYHQGKKIRPRDAFIALWTLIKYRFKKL"
     gene            1454..2215
                     /locus_tag="PLL88_11710"
     CDS             1454..2215
                     /locus_tag="PLL88_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HOJ02271.1"
                     /translation="MNNHRLFELATPVVFAHRGAKKYAPENTLASFQKALEMGAQALE
                     LDVTLSKDGEVVVIHDDRVDRTTNGTGEVTNLTLAELKELDAGAWFSQEFAGEQIPTL
                     AEVLELVQSKALINIEIKNAGKRNAELVEKTVMLVKKTKRQESIIFSSFIPGNVKKLR
                     IRLPGCPTGLLTLPGLLGKVEIGLLGRISPDLIHPHYSSVNQRFIQKQHSRFKRVHTY
                     TVNEVELMQTLAAWGVDGFFCDDLLLAQQVLTKTS"
     gene            2246..>3568
                     /locus_tag="PLL88_11715"
     CDS             2246..>3568
                     /locus_tag="PLL88_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOJ02272.1"
                     /translation="MEKKNFHLFLILSILVTLLASTGPVLANARSAQSTYTQKAQQVL
                     NKMTPEEKVGQLFLITFQGDTILEDSELVALMQDYQIGGVVLSRADDNFNGPDDTVTT
                     TAALIRQLQQNNWKNSLTDLANEEMEQTKKNYVPLFIGISQDGDSYPFDQILSGLSTQ
                     PNQMAIGATFSTELARSSGELLGQELQAMGFNLFLGPSLDVLDSSYSESDASLGTRTF
                     GGDPYWVGELGTAFISGLHSGSNSKVAVIAKHFPGRGSSDRLPEEEVATVRKSLEQLK
                     LIELAPFFATATLDANDPTTSADGFLVSHIRYQGFQGNIRETTKPVSFDSSALSLIMN
                     LQQFSSWRNAGGILVSDSLGGNAVRNFFDPNNEKFDGKQIALNAFLAGNDVLYLDGFQ
                     SSGDKSEMETIRSTLYFFAQKYREDDAFAERVDESVLRILALKFKLYEV"
BASE COUNT          892 a          793 c         1106 g          777 t
ORIGIN      
        1 gagcagcagg ggggattgat ccgcgcttcg tgccgcagcg tggaggagtt caatatcacc
       61 tatgccttag atcgctgttc cgatctgctg gtgcaatacg gcggacacag catggcagcc
      121 ggattgacgg ttgaaaaaga gaacatggcg ctgctcaaag aaaagctctg gcagattgcg
      181 cagagcgagg tagattggga agaaaccagc gcgaaactgc aactggatgc ggaagcagcg
      241 gcggtgaatg ttgccagtaa tatcacctcc atcctgaaag cactggagct gttcgaaccg
      301 accggtgagg ggaaccggcg tccgctgctt ttcagccgcg gagtgcgggt ggtcgagaag
      361 cgcgcggtgg gaaaaacggc gaaacacctg aaattgaagt taagggacgg aaacgtgttg
      421 tgggatgcca tcgcgttcgg ttttggggac aaaataaacg cgatcgacga attcgtggat
      481 gtggcatttc attttggggt gaacgatttc agtgggggag tacaactggt ggtgcgcgac
      541 ttacgcgcag ccagtcgcta gagtttcttg aagcgatatt tgatgagtgt ccacagggcg
      601 atgaaagcat cgcgcgggcg gatcttttta ccctggtgat aggcgcgcgg atgaaagcgc
      661 acaggcacct cgtaaatggg gatgcgcagc ttgatgagac gcgcggtgat ctcgggctcg
      721 atctcgaagc cgtcggaggt gagcgtgagc tgcttcaaca catcccggtg aaagaccttg
      781 taaccggtct ccatatcggt gaggtgcgtg ccgtagagca ggttggtggc gaaggtgaga
      841 aagcggttgg cgagcttgtt ccagaacagg atgccttcag ggacgcaggt gagaaagcgc
      901 gacccataca cgacctgcgc ggagcgctcg cggatgggtt cgagaagagc ggcataatct
      961 tgtgggtcat actcgaggtc ggcatcctgg atgatagcga tatccccgct ggcgtgttgc
     1021 aaaccggtgc agacggcagc gcctttgccg cggttggcgg ggtgggtgta gatgaccacg
     1081 gagggatcgt ggatggaggc gaggatggag cgggtgcggt cggaggagcc gtcgtccacg
     1141 aagagcatct cgtgcaccag gttggtggct ttcaccttct ggtaggtggt gaggatggaa
     1201 gattcttcgt tgtagacggg gatgatgacg ctgactctca tataggtgat tataaatgag
     1261 gtgcagaggg gaaacgcgcc tggtttttgg gaaaggatgt tttgggaatt ggtgagtgaa
     1321 aagttccctc tctttgtctc aggcatggga tcaggaaaaa gaaatggtat agtttattat
     1381 cagccagcac aggctgtatg gggtgagata cccatcaggg atgagtgtgc tataatgcga
     1441 atttgaggtg atcatgaaca atcatcggct gtttgaactt gccacaccgg ttgttttcgc
     1501 ccacaggggg gcgaagaagt acgcgcctga aaatacgctg gcgtcctttc aaaaagcgct
     1561 ggagatgggg gcacaggcgt tggaactgga tgtgacattg agcaaagatg gggaggtcgt
     1621 ggttatccat gatgacaggg tggaccgcac gacgaatgga acaggagagg ttaccaacct
     1681 gacgctggct gaactgaaag aattggatgc aggcgcctgg ttttctcaag aatttgcggg
     1741 tgagcagatt cctactctcg cagaggtcct ggaactggtg cagagtaagg cgctgatcaa
     1801 tattgagatc aaaaacgccg gcaagcgtaa cgcggaattg gtggaaaaaa cggtcatgct
     1861 ggtgaagaaa acgaaaagac aggagagcat catcttttcc tcattcatcc ccggcaacgt
     1921 aaaaaagctg cgcatcaggt tgcccgggtg cccaacaggg ttgttgacgc tgcccggcct
     1981 gcttggaaag gtggaaatcg gactgctggg aaggatcagc cccgacctga tccacccgca
     2041 ttattccagt gtgaatcaac gcttcatcca gaaacagcac agccgtttca agcgcgtgca
     2101 cacctacact gtcaatgaag tagaactgat gcagacgttg gctgcgtggg gcgtggatgg
     2161 attcttctgc gatgatctgt tgttggcgca acaagtgctg acaaagactt cgtaatcagg
     2221 gcatttcctt ttataattag ggttaatgga gaagaagaat tttcatctct ttttgatttt
     2281 aagtattctg gtcacattgc tcgcctcgac cgggccggtt ttggcgaatg cgcgaagtgc
     2341 acagagtacc tatactcaaa aagcgcagca agtgttgaac aaaatgacgc ccgaagaaaa
     2401 ggtagggcag ttgtttctga tcacatttca gggggatacc atacttgaag attcagagct
     2461 cgttgccctg atgcaagatt accagatcgg gggagtggtg ctctcgagag cggatgataa
     2521 tttcaacgga ccggatgata cggtgacgac cacagcggct ttgatcagac agttgcagca
     2581 aaacaactgg aaaaacagcc tgacagatct tgccaatgaa gagatggaac agaccaagaa
     2641 gaattatgtt cccttattca taggcatctc acaagatgga gattcttacc cctttgacca
     2701 gatcctctcg ggcttatcca cgcagcccaa ccaaatggcg atcggggcga cgttttccac
     2761 cgaactagcc aggtcgagcg gggaactgct ggggcaggag ctgcaagcca tggggttcaa
     2821 cctgtttctc ggaccatcgc tagatgtgtt ggattccagc tattcagaaa gtgatgccag
     2881 cctgggcacg cgcactttcg gcggagatcc ctactgggtg ggcgaattag gcaccgcctt
     2941 catcagcggg ctgcacagcg gaagcaattc caaagtggcc gtgattgcca aacactttcc
     3001 gggcagaggc agttccgatc gcttgccgga agaagaggtg gcgacggtac gaaagtcgct
     3061 ggaacagctc aagctgatcg agctagcgcc attcttcgcg accgccacgc ttgatgcgaa
     3121 tgacccgaca acgagcgcag atggctttct ggtaagccac atccgctacc agggatttca
     3181 aggaaacatc cgcgagacga ccaaaccggt cagtttcgac tcatcggcgc tcagcctgat
     3241 catgaacctg cagcagttca gcagttggcg caacgccggg ggaattctgg tgtcagacag
     3301 tttgggcggc aatgcggtca ggaatttctt tgacccgaac aatgaaaagt tcgatggcaa
     3361 acagatcgcg ctgaacgcat ttctggcggg gaacgatgtg ctctatctag atggtttcca
     3421 atccagtgga gataaaagtg aaatggagac tatcagaagc acgctatatt tcttcgcgca
     3481 gaaatatcgc gaagacgacg cgtttgctga acgggtggat gaatcggtat tgagaattct
     3541 ggcgttgaaa tttaagctct atgaggtg
//
LOCUS       DAOSDS010000167         3537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_9318_length_3537_cov_3.789202, whole genome shotgun sequence.
ACCESSION   DAOSDS010000167 DAOSDS010000000
VERSION     DAOSDS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9318_length_3537_cov_3.789202"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLL88_11720"
     CDS             complement(<1..431)
                     /locus_tag="PLL88_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02273.1"
                     /translation="MKKAWQIDREMRENAIPVDRRVQSSNWYEAGFEVPYGDALIDLF
                     WESKVPGSSAPESSYVSMVQAMGNKGYDVSTAEQYLEEGLHLHAEGRKDELRVITAKV
                     LNALQNAPLDRNESYHTYQHPESWEDVQKSMGAVSAVPERK"
     gene            complement(572..1033)
                     /gene="bcp"
                     /locus_tag="PLL88_11725"
     CDS             complement(572..1033)
                     /gene="bcp"
                     /locus_tag="PLL88_11725"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOJ02274.1"
                     /translation="MLKEKTKAPDFELQDENGVPHKLSDYAGAPLVLYFYPKDDTPGC
                     TTEACSFRDDYSAFEQAHTKIVGVSTDSVESHKKFKNKYGLPFPLLSDPDHKVVEQYE
                     VWAEKRMMGKKYMGILRTTYLIDKDGNIARVFEGVKPAEHSQEVLEAIKQL"
     gene            complement(1101..1988)
                     /locus_tag="PLL88_11730"
     CDS             complement(1101..1988)
                     /locus_tag="PLL88_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02275.1"
                     /translation="MKKRTDPLFTQFYSNVRVADFILSFSFFLFGSIYCDYVGIPINV
                     TRLCWGLLVILFLLVSMEFLNLFFREERDFEDALLQRFGAFDYRMIFFLLAVACILVA
                     AVICVFQIRTATALIIAALMLMVVMLYIAKPFRLIYSGYGEVIHAFLITFLIPAFGYS
                     IQAKGGLHITLAYLCIPFFVLVLAHQFIAENRSLARDIKQYHTTAVMRFGSVLTLRFA
                     MYLIAFSFIFILLLGVGTLPWRFIVRWYVGIPLAIYLLWNVNKVLSGEKPAWQLISFL
                     SYSLILLNLMLILFSFILV"
     gene            2045..3196
                     /locus_tag="PLL88_11735"
     CDS             2045..3196
                     /locus_tag="PLL88_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02276.1"
                     /translation="MQRKEKKARSRKGLFPLMPLLPLLLAASLFQGIIVIFSEIHLPI
                     LLIDMLISIFYCSILVLEIWGAYRDQPFKDKNDVLKLVGWYLTARPLITVNVENGKIL
                     CDGPLADQRKLLTIDPASAAAIRTPSDPQIQIFPAGMYCLEKGQTIFSTFDLRTQTSV
                     SPTIPREDEISLNDPLLTGSLRFASRSSFSVATTADGYQLGARFLVRYKYDIEFGEGE
                     NPYGFDAAILRKVHQADSIERGSFLDSQHISQERIQATLQSIWQSSISQFALLDVIPQ
                     EQNQPSKLEEIELEIRRQVENAPLSSLEKEAPAERLRSYGLRVLTLSILSIWLPEETE
                     IAIQHHWQPQIQQLLNGLQSMQQHKTALFQELGEVYALYESLANRGSGH"
     gene            3198..>3537
                     /locus_tag="PLL88_11740"
     CDS             3198..>3537
                     /locus_tag="PLL88_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02277.1"
                     /translation="MKPSAFSLVNASAYQQFRTQKWQTWVIRISLTLITLVSLFTLIF
                     DSTVQKWAATRGELSITAQVELLKKIVNPLSVLAAIFFLCMVAYSAIRSVRLQGIEGR
                     SPELFANLPEK"
BASE COUNT          961 a          884 c          794 g          898 t
ORIGIN      
        1 tctttgcgtt caggcacagc gctcacagca cccatgcttt tttgaacgtc ttcccagctt
       61 tcaggatgct gatatgtatg ataactctcg tttcgatcca gcggtgcatt ctggagggcg
      121 ttcagcactt ttgcagtgat cacgcgcaac tcgtcttttc tcccctctgc atgcagatgc
      181 aaaccctctt ccaaatattg ctcagcagtg ctcacgtcgt accctttgtt ccccattgcc
      241 tgcaccatgg aaacatagct ggattccggg gcagatgagc cgggtacctt agattcccag
      301 aacagatcaa tcagcgcatc gccataggga acttcaaagc ctgcctcata ccagttgctg
      361 gattggacgc gcctgtcgac tggaatcgca ttttcgcgca tttctcgatc gatttgccac
      421 gctttcttca tggacatttc tcctctggaa tatttaacta ttttagaaaa ccaactttag
      481 tatatatgat tttaaacaac atttagcatg aagagcgtac ccgcccgcat gctaaatgtg
      541 atacaaaaat cctccaccct accagggaaa atcagagttg ttttatcgcc tccagcactt
      601 cctggctgtg ttcggctggc ttgacgccct cgaaaacacg agcaatattt ccatctttgt
      661 cgatcaggta cgtcgtgcgc agaattccca tatacttctt gcccatcatc cgtttttccg
      721 cccacacttc atattgctcg acgaccttat gatcgggatc ggataataat ggaaaaggca
      781 agccgtattt gttcttgaac tttttatgag attccaccga atccgtactg acacccacaa
      841 tcttggtatg cgcctgttca aacgcgctgt agtcatcgcg gaaactgcag gcttcggtcg
      901 tgcaacccgg tgtgtcgtct tttgggtaaa aatacaaaac cagcggagct cccgcataat
      961 cagacagctt atgaggcact ccattttcat cctgaagctc aaagtcaggg gcttttgtct
     1021 tttcttttaa cattttgtta tctcctttcc cccattataa gagagacggt tttgaactat
     1081 cgttaatgaa acataaaaaa tcagaccaag ataaatgaaa acaaaataag cattagattc
     1141 aacaggatca gggaataaga caagaaactg atcaattgcc aggcaggttt ctctccgcta
     1201 agaaccttgt tcacattcca gagcaaataa atggctagcg ggatgccgac gtaccagcgc
     1261 acaatgaatc gccatggaag ggttccgaca cccaacaaca agatgaagat gaacgaaaat
     1321 gcgatcaggt acatggcgaa gcgcagagtc agcacacttc caaagcgcat cactgccgtt
     1381 gtgtggtact gcttgatatc acgcgcgagc gagcggttct ctgcgatgaa ctggtgggct
     1441 agcaccaaca cgaagaacgg gatgcagaga tacgccaatg tgatatgcag accccccttt
     1501 gcctggatgg aatagccaaa agcggggatg agaaacgtga tcaaaaaagc gtgaatgacc
     1561 tcgccatagc cagagtagat cagacggaaa ggtttggcaa tatacagcat gacgaccatg
     1621 agcatcaatg cggcgatgat cagagccgtt gcagtacgga tctgaaaaac gcagatgacc
     1681 gcagcaacga ggatacatgc aaccgccagc aggaaaaaaa tcatgcgata atcgaaagct
     1741 ccaaatcgtt ggagcaatgc atcctcgaag tctctctctt cacgaaagaa caggttgaga
     1801 aattccatgg ataccagcaa gaacaggatc accagcaacc cccaacaaag ccgggtgacg
     1861 ttgatgggaa tgccgacata atcacaatag atggatccga agagaaaaaa gctgaatgac
     1921 agaatgaaat cagcaacgcg cacattggaa taaaactgtg taaacagagg gtcggttctt
     1981 ttcttcactt cttttccttc cagtttattt tctttgttct cattggatga gatacaatgt
     2041 acctatgcag cgcaaagaaa aaaaagctcg gtcgagaaag ggtctttttc ctctcatgcc
     2101 cttgctgcca ttgttgcttg ccgcatcgtt gttccagggt attatagtca ttttttctga
     2161 aatccatctt cccatcctgc tgattgatat gttgatctcc attttctatt gtagcattct
     2221 ggttctggaa atctgggggg catatcgtga tcaacccttc aaagataaaa acgatgtgct
     2281 taaattggtg ggatggtacc tgacagcgcg tccattgata acggtcaacg tggagaatgg
     2341 gaaaatacta tgcgatggtc cgttggcaga tcaacgcaaa ctgctaacca tcgatcccgc
     2401 cagcgcggct gccattcgaa ccccctccga tccacagatc cagatatttc ctgcaggtat
     2461 gtattgcctg gaaaaagggc aaaccatctt ctcgacattc gatctgcgca cacaaaccag
     2521 cgtttctccg accatcccca gagaagatga gatttctctg aacgatcctc ttttgacagg
     2581 atctttacgc tttgcctctc gttcctcttt ttcagtggca accaccgcag acggttacca
     2641 gttgggagcc agatttctgg ttcggtataa atacgatatt gaatttggag aaggcgaaaa
     2701 cccctacggc tttgatgcag ccattttgcg caaggtgcac caggctgatt ccatcgagcg
     2761 cggttctttc ctggattccc agcatatctc gcaggaacgt attcaagcaa cactgcaatc
     2821 aatctggcag tcttccataa gccagttcgc actgcttgac gtgattcccc aagaacagaa
     2881 tcagccttcc aaacttgaag agatcgagct ggaaatacgg cgtcaggtag aaaacgcacc
     2941 gttgtcttct ttggaaaagg aagctcctgc ggagcgattg cgcagttacg gtctgagagt
     3001 tttaacgcta tccatccttt ccatctggtt gccggaggag accgaaatcg ccatccaaca
     3061 tcactggcag ccacaaatcc aacaactttt gaacggatta cagagcatgc aacagcacaa
     3121 gaccgcactc ttccaggaac tgggtgaagt gtacgccttg tacgaatccc tggcgaatcg
     3181 tgggagcggg cattgacatg aaaccatcgg cattttctct ggtgaacgcg agcgcctatc
     3241 agcaatttcg aacacaaaaa tggcagacgt gggtcatcag gatctctttg acgctgataa
     3301 cgttggtttc attatttacc cttatctttg attcaaccgt gcagaagtgg gcagcaacca
     3361 ggggggaact ttcgatcacc gctcaggtag aactgcttaa aaagatcgtc aatccactca
     3421 gcgttctggc tgctatcttc tttctttgta tggtcgctta ttccgcaatc cgatctgtac
     3481 ggttgcaggg aatcgaaggt cgtagccctg aactgttcgc taatctaccg gagaaag
//
LOCUS       DAOSDS010000168         3446 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_9668_length_3446_cov_3.588322, whole genome shotgun sequence.
ACCESSION   DAOSDS010000168 DAOSDS010000000
VERSION     DAOSDS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9668_length_3446_cov_3.588322"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3446
                     /gene="rpoC"
                     /locus_tag="PLL88_11745"
     CDS             <1..>3446
                     /gene="rpoC"
                     /locus_tag="PLL88_11745"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOJ02278.1"
                     /translation="DSRIEAVEQDKNSRLDEIKGKTTELSAKKDEMIAEKVAPLIKEG
                     QELEQSIKEKVGKKARADIKLKTINETIASAGEEITNKLISNMQKLVQKKLEAIEAEI
                     GIEIEHEKEKNDLLMEQVKAEADLTLQKLLDQKEDLTAKIEKEIAARRDEIEGLRPLS
                     FISENKYRELRSHYGQVFRADMGAEAFYDILSRLDMEKLAEELWVEVRTTRSKQKRKK
                     ATKRLKVVEAFRRSNNRPEWMILKVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLK
                     GKKGRFRRNLLGKRVDYSGRSVIVVGPKLKLYQCGLPKNMALELFRPFVIAGLVQREY
                     AANVKGARRLIERNRPEVWEVLERVIKERPVMLNRAPTLHRLGIQAFEPILVEGSAIQ
                     LHPLVCTAFNADFDGDQMAVHVPLSEKAVWEARNLMLASKNLLKPADGEPIISPSKDM
                     VLGVYYLTMDAEKTKGDVTTFASIEEVELAYQLELVKVHTPIQLRTKTWFGEQGERFS
                     KQETRIIKTTVGRALFNQILPEEVVFVNETLDKGGVKDLIATIYDLCGEETTTRITDD
                     IKDIGFKYAMLSGITLAVGDISVPPQKEQILTESLEKVETIQKSYRRGLLTELEMDEQ
                     VIKVWQDTTKEVAEAVKEHLDPDGTLATMANSGATKGGFGPISQLAGMRGLMADPAGR
                     IIRLPIQSNFREGLTALEYFISTHGARKGQADTALRTADAGYLTRRLVDIAQDVIINA
                     EDCETQEGLWVRGKDDVAGQSIQERLKGRLLAEAIADPKTGEILADHDEPLTNEVIVK
                     LLDAGITEFKVRSPLTCELQHGICAKCYGIDLGRGKMVETGTAVGIIAAQSIGEPGTQ
                     LTLRTFHTGGVAAATDITTGLPRVEELFEARKTPKGEAVIAPIMGKVAIIPSNTASDQ
                     NSIRITYSELLRDEYTVPEDWEIVVKDEATVAVGDVLAKNDELTINAQHAGRVRVEKR
                     TVIVGYDSVEVEEMDIPSTLRITVKDGENVEAGQPLTEGSINPHELLKLKGRDACYYY
                     LLKEIQNVYRAQGQNINDKHFEVIIRKMLSKVQIINAGDTEFLPNEMIDRLRIKAINE
                     EM"
BASE COUNT         1006 a          764 c          929 g          747 t
ORIGIN      
        1 tggatagtcg tattgaggcg gtcgaacaag ataaaaattc cagattggat gagatcaagg
       61 gcaagacaac ggaactttct gctaaaaaag atgaaatgat agcggagaag gttgccccat
      121 tgatcaaaga ggggcaggaa cttgaacaat ctatcaaaga aaaggttggc aagaaagctc
      181 gcgcagatat caaactaaag acaatcaacg aaacgatcgc cagtgctgga gaggaaatca
      241 ccaataaact gatctccaat atgcaaaaac tggtccagaa aaagctcgaa gcgatcgaag
      301 ctgagatcgg cattgagatc gaacacgaaa aagagaagaa cgatcttctg atggaacaag
      361 tgaaggctga agcggatctg accctgcaaa aattattaga ccagaaagag gatctgacag
      421 ccaagattga gaaagagatc gcggctcgac gtgacgaaat tgagggattg agaccacttt
      481 ctttcatcag cgaaaataaa tatcgcgaac tgcgttcaca ttatgggcag gtttttcgcg
      541 ctgatatggg cgccgaggcg ttctacgata ttctcagccg tctggacatg gagaaactgg
      601 ctgaagagtt atgggttgaa gtgcgcacca cgcgcagcaa acaaaaacgg aaaaaagcga
      661 ccaaacgctt gaaagtggtt gaagcattcc gccgttcaaa taacagacct gaatggatga
      721 tcttgaaagt tctgccggtc atcccgcccg atttgcgacc catggtgcag ttggatggag
      781 gtcgctttgc gacttctgac ctgaatgacc tgtaccgacg agtgatcaat cgcaataatc
      841 gcttgaaaag gttgctggaa ttgggtgctc cagatgtcat cgtgcgcaat gaaaaacgta
      901 tgctccagga agcggttgat tctctgatcg ataattcaca acgaggaaaa gctctttctc
      961 gacgcggacg gcgcgagctg aagtcgttaa gcgacatgct gaaagggaag aaaggtcgct
     1021 tccgccgcaa tttattagga aagcgtgtag attactctgg tcgatccgtg atcgtggtgg
     1081 gtccgaagct gaagttgtac caatgcggtt tacccaagaa tatggctttg gaactgttca
     1141 gaccattcgt tattgccggg ctggtacaac gtgagtacgc cgccaatgtg aaaggagccc
     1201 gccgattaat tgaacgaaat cgacctgaag tgtgggaggt tttagagcgg gttatcaaag
     1261 aacgaccggt catgctgaac cgcgctccaa ccctgcaccg cctgggtatt caggcatttg
     1321 aaccaatttt ggtagaaggc agtgcaattc aactgcatcc gctggtgtgt actgcgttca
     1381 atgcagattt tgatggtgac cagatggcgg tgcacgtgcc attatctgag aaggcggttt
     1441 gggaagccag aaacttgatg ctggcgtcaa agaacttgct gaaacctgca gatggcgaac
     1501 cgatcatctc gccttccaaa gacatggttc tgggcgtgta ttacctgacc atggatgcgg
     1561 aaaagacaaa aggcgatgtg acaacctttg catccattga agaagttgaa ttagcttacc
     1621 aattggagct ggtgaaggtg catactccca tccagctacg aaccaaaacc tggttcgggg
     1681 agcagggcga acgtttcagt aagcaagaaa cccggatcat taaaaccacg gtagggcgtg
     1741 ctctcttcaa ccagattttg cctgaagagg tggtgttcgt aaacgaaacg ttggataaag
     1801 gtggagtcaa ggacctgatc gcgacgattt atgatctatg cggtgaagaa accactacca
     1861 ggatcaccga tgatatcaaa gatatcggat tcaaatatgc catgctttca gggataaccc
     1921 tggcggttgg cgacatcagt gtcccaccgc aaaaagagca aattttgacc gagtcccttg
     1981 aaaaagttga gaccattcaa aaatcttacc gacgtggtct gctcaccgaa ctcgagatgg
     2041 atgagcaagt catcaaagtt tggcaggata ctacgaagga agttgcagaa gcggtaaaag
     2101 aacacctgga cccagatggc accctggcaa ccatggcgaa ttctggggct accaagggtg
     2161 gttttggacc tatctcgcaa ttggcaggta tgcgtggttt gatggctgac cctgccggtc
     2221 gcattatccg cttgcccatt caatccaatt tccgtgaagg gttgaccgcg ctggagtatt
     2281 tcatttccac ccatggcgcc cgaaaaggac aggctgatac cgctttgcgc acagcagatg
     2341 cgggttatct gacccgacga ctggttgaca ttgcgcagga cgtcatcatc aatgctgaag
     2401 attgcgagac gcaagaagga ttatgggtca ggggcaagga tgatgtagcc gggcagagca
     2461 ttcaagaacg gctcaaagga cgcttgctgg ctgaggcaat tgctgatcct aagacgggag
     2521 agatccttgc tgatcatgat gaacctctta cgaatgaagt gattgtcaaa ctgctcgatg
     2581 caggtatcac tgagttcaaa gttcgttccc cgttgacctg tgaactacag catgggattt
     2641 gcgcaaagtg ctatgggatc gacttgggtc gcggaaagat ggtagaaaca ggaacagcgg
     2701 ttggcatcat cgccgcgcag tcgattggtg aaccaggcac gcaacttacc ctgagaacat
     2761 tccatacggg cggcgttgcc gcggcaaccg atattacgac aggtctgccc agggtcgagg
     2821 aactgttcga agcccgcaag acccctaaag gagaagcggt catcgcccct atcatgggga
     2881 aggtggctat catcccctcc aataccgcta gcgatcagaa cagcatccgc atcacgtata
     2941 gcgaactgtt gcgtgatgag tacaccgtac cggaagactg ggaaatcgtt gtcaaggatg
     3001 aagccacagt cgcggttggc gatgtgcttg ccaagaacga tgagcttacc atcaacgcac
     3061 agcatgcagg tcgcgtgcgc gttgaaaaac gcacagtgat tgtcggctac gacagtgtgg
     3121 aagtcgaaga gatggatatt ccgtcaacac tacgcatcac cgtaaaagat ggagaaaatg
     3181 ttgaagccgg tcaaccattg acagaaggtt ccatcaaccc acatgaattg ttgaagttaa
     3241 aaggacgaga tgcctgctat tactatctgc tgaaagaaat ccaaaatgtg tatcgtgccc
     3301 aaggtcaaaa tatcaacgat aaacacttcg aagtgattat ccgcaagatg ctgagcaaag
     3361 tgcagatcat caacgccgga gatactgagt ttctacccaa tgaaatgatc gatcgactgc
     3421 gcatcaaagc gatcaacgaa gaaatg
//
LOCUS       DAOSDS010000169         3276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_10335_length_3276_cov_4.203043, whole genome shotgun sequence.
ACCESSION   DAOSDS010000169 DAOSDS010000000
VERSION     DAOSDS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10335_length_3276_cov_4.203043"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="PLL88_11750"
     CDS             complement(<1..165)
                     /locus_tag="PLL88_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ02279.1"
                     /translation="MTQNFIRVAGAREHNLKNISVDIPRDKLVVITGLSGSGKSSLAF
                     DTIFAEGQRRY"
     gene            complement(303..1175)
                     /locus_tag="PLL88_11755"
     CDS             complement(303..1175)
                     /locus_tag="PLL88_11755"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ02280.1"
                     /translation="MIRKPVLGLALGGGGVRGFAHIGVLKVLRSEDIAIDCLSGTSMG
                     GIIAALTACGLTIEEIEETARRVSSLGELAKLMDTRITKLDHIFKSESIQEFFEETIG
                     KDPQFSDLKIPLALSAVDFDLAREVVLQKGALIPAINATMGLPGIVEEVKIDGRTLVD
                     GGSLNNVPADYVRSMGAEIVIAVDVSPSIEDASYWKNQKMPGIASGYWRANAMMGANI
                     TEAKLKKANVDFLIRPAIGSDVATLGGFSQVGQVIAAGAKAAWDVMPDLKKVLKERFF
                     FSKPVIKPAEAVLL"
     gene            complement(1316..3250)
                     /gene="gyrB"
                     /locus_tag="PLL88_11760"
     CDS             complement(1316..3250)
                     /gene="gyrB"
                     /locus_tag="PLL88_11760"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOJ02281.1"
                     /translation="MEDGLIERTKSATSYGAKDIQVLEGLEAVRRRPGMYVGGTDIKA
                     LHHLVYEVVDNSIDEALAGACNQIVVTINKDSSVTVMDNGRGIPVEEHPTKKVSALEV
                     VMTILHAGGKFGGTSYKVSGGLHGVGVSAVNALSEWCEVRVKRDGKMYFQRYERGIPH
                     EPVKMIGKCDPEDTGTITTFRFDEEIFEKDINYRFETLAQRFREMAFVTRQVEICLVD
                     NRSENSRRVTYYFEGGIVSFVHYLNRNREILHDVFYVEGGENGVGIEVAMQYTDAYAE
                     SVYAFANTINTIDGGTHLTGFRSSITRVINDYARKNGMIKDSEGNFTGDDVREGMTAI
                     ISVKHPNPQFESQTKVKLMNADVQTLVQQIVGEGFAEFLEENPSAARAIINKCLTSAR
                     ARSAARKARDLVIRKSALESLTLPGKLADCSERNPEKTELYIVEGDSAGGSAKQGRDR
                     HFQAILPLRGKILNTERSRLDKILSNKEVKALISALGTGIGDGFDLGNLRYGKIIIMT
                     DADVDGSHIRTLLLTFFFRFMSDLIENGHLFIAQPPLYLIRNKNEKRYAYTDEGKDAI
                     LKELGTKSSIQRYKGLGEMNPEQLWDTTMNPENRTLLLVTIEDAAHADQTFDMLMGSL
                     VPPRKRFIQTHAKYVRNLDI"
BASE COUNT          831 a          860 c          757 g          828 t
ORIGIN      
        1 atagcgccgt tgcccttcag caaaaatggt atcaaaggca agggaactct tgccagaacc
       61 ggataagccg gtgatgacca ccaatttatc gcggggaata tcaaccgata tattcttcaa
      121 gttatgttcg cgcgcacctg ccacgcgaat aaaattttgt gtcatgaacg atcctgattt
      181 atgaaatgaa taattattat atcatttgtt ctattaattt tttagagcag tgcggcattg
      241 ctaataaaaa atcctccttt acagaaccaa ggaggatccg ttcaggaaaa ataaggaaac
      301 gattagagca acaccgcttc ggctggtttt atgacaggtt ttgagaagaa gaagcgttct
      361 ttaaggactt tcttcaggtc aggcatgaca tcccaggcag ctttggctcc cgcagcgatg
      421 acctgaccga cctgactgaa cccgcccagg gtagccacat cagaaccgat ggcaggcctg
      481 atcaaaaaat caacattggc ttttttcagc ttggcttcag tgatgttcgc tcccatcatg
      541 gcattagcgc gccagtagcc gctggcaatg ccgggcattt tttgattctt ccaataggag
      601 gcatcttcga tggaggggct aacatccacg gcaatcacaa tttctgcgcc catagagcgc
      661 acataatcag ccgggacatt attcaacgaa ccgccatcca ccagggttcg cccgtcgatt
      721 ttcacttctt ccacaatacc aggcaacccc atggtggcat tgatagcagg gatcaacgcc
      781 cctttctgca aaaccacttc tctggcaagg tcgaaatcca ccgcacttaa tgccagaggg
      841 attttgaggt cggagaattg cggatccttt ccgatcgttt cttcaaagaa ttcctgaatg
      901 ctctctgatt tgaaaatgtg atccagtttg gtgatgcgcg tgtccatcaa tttggctaat
      961 tcgcccaaac tggacacgcg ccgggcagtc tcctcgatct cttcaatagt aagaccacag
     1021 gcagtcaaag ccgcaatgat gccgcccatg ctggttccgc tcaggcagtc aatcgcgatg
     1081 tcctcagagc gcaaaacttt caacacacca atgtgcgcga aaccccgcac tcctccgcca
     1141 cccagggcaa gtcccaaaac tggttttcgt atcataaatt ctcagcacat ccttcaataa
     1201 actcagtgca attataatag cgattccctg ttcgccagaa cacttcgcct ttttcaaagg
     1261 gggaaatgcc agctttcatc gcattccagc agcacgaatt caacaagcgt ggcttctata
     1321 tatccagatt ccgcacatac ttggcatgcg tctgtatgaa acgcttgcgc gggggaacca
     1381 gtgaacccat cagcatatca aaggtttgat cagcatgggc agcatcttca atagtgacca
     1441 gcagtagggt acggttttcc ggattcatcg tggtatccca cagttgttcc gggttcattt
     1501 cacccagacc tttatagcgc tgaatactgg attttgtacc caattccttc aagatggcat
     1561 ctttgccttc gtctgtataa gcgtagcgct tttcattctt attcctgatc aaatagaggg
     1621 gtggctgggc gatgaacagg tgcccattct cgatgagatc cgacataaag cggaagaaaa
     1681 aggtgagcag cagggtacga atatggctgc catccacatc ggcatcggtc atgatgatga
     1741 tcttgccata gcgcaagttt ccaagatcaa aaccgtctcc aattccggtt cccagcgcag
     1801 aaatcaaggc ttttacctct ttgttggaga ggatcttatc caaacgggaa cgttcggtat
     1861 tcaggatctt gccgcgcaag ggaaggatag cctgaaaatg gcggtcgcga ccctgtttcg
     1921 ccgaaccgcc ggcgctatca ccttctacga tgtacaattc agtcttttca ggattgcgtt
     1981 ccgagcaatc agccagcttg ccgggcaggg tcaagctttc cagcgcagat ttgcgaatga
     2041 ccagatcgcg cgccttgcgc gcggcagagc gagcgcgggc ggaggtgaga catttattga
     2101 tgatagctct ggctgctgat ggattctctt ccaagaattc cgcaaagccc tcacctacga
     2161 tctgctggac aagggtttgc acatcggcat tcatcaactt cactttggtc tggctttcaa
     2221 actgcggatt ggggtgcttg acacttataa tagcggtcat gccctcgcga acatcatcgc
     2281 cggtgaagtt gccctcgcta tctttgatca tgccgttctt gcgggcgtaa tcattgataa
     2341 cacgcgtaat ggatgagcgg aaacccgtga gatgggtacc accatcaatg gtattgatgg
     2401 tgttggcgaa tgcataaacc gattcggcat aggcatcggt gtattgcatg gcaacttcga
     2461 tgccgacccc attctctccc ccttccacat agaagacatc gtgcaatatc tcgcgattgc
     2521 gattcaaata gtgtacgaat gagacgatac caccctcaaa ataatacgtg acacggcggg
     2581 aattttcaga gcggttgtcc accaggcaga tctctacttg cctggtgaca aaagccattt
     2641 cacgaaaacg ctgggctaac gtttcgaacc ggtaattaat atctttctca aagatctctt
     2701 cgtcaaagcg gaaagtagtg atggtgccgg tgtcttcagg atcgcacttg ccgatcatct
     2761 tgaccggctc gtggggaatt ccgcgttcat agcgctggaa gtacatcttg ccatctcttt
     2821 tcacgcggac ctcacaccat tctgaaagcg cgttgacggc agaaacgccc acaccatgca
     2881 aaccgccaga taccttgtag ctggtgccgc cgaacttgcc accggcgtgc aaaatcgtca
     2941 tgaccacttc aagagcagaa accttcttgg tgggatgttc ttccaccggg ataccacgcc
     3001 cattatccat aacggtaacg ctactatctt tattgatggt caccacaatc tgattgcaag
     3061 cgccggctag cgcttcatcg attgaattat cgaccacttc atatacaaga tggtgcaggg
     3121 ctttgatatc ggtaccacca acatacatgc cagggcggcg gcggaccgct tcaagcccct
     3181 ccagaacctg gatatctttt gcaccgtatg acgtggcgct ctttgttctt tctatcaaac
     3241 catcctccaa aatgagtata ttttcaaaat cacagt
//
LOCUS       DAOSDS010000170         3183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_10741_length_3183_cov_4.346867, whole genome shotgun sequence.
ACCESSION   DAOSDS010000170 DAOSDS010000000
VERSION     DAOSDS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10741_length_3183_cov_4.346867"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..>865
                     /locus_tag="PLL88_11765"
     CDS             253..>865
                     /locus_tag="PLL88_11765"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ02282.1"
                     /translation="MAIDKISIIRKNQLPQQHTISVIIPIYNEEKSLFTFLQKLCAFK
                     NIDEIICVNDGSEDCTADILRCFSNQVHCIHFQTNQGKGSALAAGVRACHGDIVVFLD
                     ADLLNLTQTHFQLLINPLLEKKKKAVIGYCKSSEFHFVLTEFISGQRAYFKNDLLPYL
                     DEMECMRYGVEMFLNSLLHPFDVEFVPLMDLRSIYKFEKYDSSL"
     assembly_gap    866..875
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1146..1217)
                     /locus_tag="PLL88_11770"
     tRNA            complement(1146..1217)
                     /locus_tag="PLL88_11770"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1183..1185),aa:Lys,seq:ttt)
     gene            complement(1277..1570)
                     /locus_tag="PLL88_11775"
     CDS             complement(1277..1570)
                     /locus_tag="PLL88_11775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HOJ02283.1"
                     /translation="MRTYSEPHSMIPCSECNIGQMHKTSMTYFTWLNGEMITVPDFPA
                     WVCDICGKREYDHEAVEQLSLLLNPASATSRAHSRLARQSEQTRDKQRTTPID"
     gene            complement(1678..1956)
                     /locus_tag="PLL88_11780"
     CDS             complement(1678..1956)
                     /locus_tag="PLL88_11780"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOJ02284.1"
                     /translation="MIIGKVVGTVVTTISHAHYKDRRLLVVEPLFVEGDKTGGDFLAL
                     DNSHAGIGDTVLVLREGSGARQVMNNPDACVISVIVGIVDSVSVEYSV"
     gene            complement(1974..2294)
                     /locus_tag="PLL88_11785"
     CDS             complement(1974..2294)
                     /locus_tag="PLL88_11785"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOJ02285.1"
                     /translation="MLFGKVKGTAICTLKYQDLEGLKFLIVQPLDKRLNPVGGLQVAV
                     DVVQAGSGDLCVMVRSREAALAMPGVKFVPVDLALVGIVDELEVKPDGEFDFTLKRGW
                     NTYT"
     gene            complement(2297..2659)
                     /locus_tag="PLL88_11790"
     CDS             complement(2297..2659)
                     /locus_tag="PLL88_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02286.1"
                     /translation="MAKTFYTERDVEDLFRQGTMTITLTDDVIMTDLAYEKARKLEMR
                     IAQAEETPPAAPIRPYLNTPVQSATVSAPCKCAEASRVAEIKAKVHETVKARLGNSVD
                     EAMLDAVIERVAKDVGLG"
     gene            complement(2674..>3183)
                     /locus_tag="PLL88_11795"
     CDS             complement(2674..>3183)
                     /locus_tag="PLL88_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02287.1"
                     /translation="LYIEMAPGNEVFRTVDIAVKATEAKPGAQIVEREFGMFEVHSMN
                     QAAVIEAGNVVLDRLGLKVEDRVKPQIASVQVITRVDSYQAQLLNRFRRGSMLVPGET
                     MLVLECAPAAYINYACNEAEKRATIKIVHVSSVGRFGRMWLSGSEAEIINAKNAAIQA
                     LENLEGQAE"
BASE COUNT          848 a          817 c          653 g          855 t
ORIGIN      
        1 gggtgaaaac caataaaaaa ggaaaactcc tctctgcgaa acgcggttgt gatatacgac
       61 cgcgtttctt cattaaaagt atagacggat attcatttcc cttttccagc atcacattcg
      121 aaaggaaaat atcctgaaca aaaccaaaaa gtaaacgatt acgatcctca cttaccttcg
      181 acatgaaccg gtttctctaa tggaaaatta atcattttca agtacattaa catgcataat
      241 tggagatatt ttatggcgat cgataaaatt tctattatca ggaaaaatca actaccgcaa
      301 caacacacta tttctgtaat cattccaatt tacaacgaag aaaagtctct ttttactttc
      361 cttcaaaagc tatgcgcctt taagaacatt gatgaaatca tttgtgtaaa tgatggttca
      421 gaggattgca ccgctgacat tctccgttgt ttctcaaatc aggtccattg catccatttt
      481 caaaccaacc aagggaaagg atcggctctt gcggctgggg ttagagcatg ccatggtgac
      541 attgtagttt ttctggatgc agatctgttg aatctaaccc aaacgcattt ccaactgctg
      601 atcaatccgc ttctcgagaa gaaaaagaaa gcagtcattg gctattgtaa atcttcagag
      661 ttccatttcg tgttgactga gtttatctca gggcaacggg catattttaa aaacgatctg
      721 ctaccttatc ttgatgaaat ggaatgcatg cggtatggag ttgaaatgtt tcttaactcc
      781 ctccttcatc catttgatgt agagtttgtt cctttaatgg atctccgttc catttataaa
      841 tttgagaaat acgactcctc gctggnnnnn nnnnnggttg ccaaacaata ctaccttgag
      901 ggaaaagaaa tcgtccagtc actgccgaaa accgtcctta ccaaacgatt tgctggtctc
      961 ttgttggatc ggtttagaga tatccttccc gttgtatagg tatcctcaga aaaatctagc
     1021 tgattgaaaa ttggttcatg attcgttatg cttgggatat aagtagggaa acagtcctcc
     1081 ttacaaaggg aggaagacta tgcaaaaaag acaatttcac taatcataaa cttttaaaac
     1141 aaaaatgagc gcggagagga tcgaactctc aaccaatagc ttaaaaggct actgctctgc
     1201 cattgagcta cgcgccccaa gagtgcttat tttaacatgc acgagcataa gcgtcaacga
     1261 attggtagat gaaaccttag tcaatggggg ttgtgcgttg tttgtcgcgg gtctgttcgc
     1321 tttggcgcgc gagacgtgaa tgcgcgcggc ttgtagcgct ggcaggattt agtaaaagcg
     1381 aaagctgttc aacagcttcg tggtcatact cgcgctttcc gcaaatatcg cagacccaag
     1441 ccggaaaatc aggcactgtg atcatctccc cattcaacca ggtgaaatag gtcatgctgg
     1501 tcttatgcat ctgcccaatg ttgcattcgc tacatgggat catactgtgc ggttcactgt
     1561 atgttctcat caaagcttcg ccctcgatat tgtaatattc ctattcttat cgaaattata
     1621 ccagtttgtc agatcaaaca agaaaagtct gttcagacaa tgaactccct tcagatatta
     1681 aacggagtat tcaacggaga cagaatccac aatgcccaca atcacagaga taacacacgc
     1741 atccggattg ttcatcacct gacgagcgcc gctcccttca cgcagcacaa gcaccgtatc
     1801 accaatgcca gcatggctgt tatcgagagc caaaaaatcc cctcccgttt tatctccttc
     1861 gacaaacagc ggctctacca ccaacaaacg ccggtcttta taatgcgcat ggctgatggt
     1921 cgtgactacg gttcctacca cctttccaat gatcatggtt gtccctttat tctttaggta
     1981 taggtgttcc acccacgttt gagcgtgaaa tcaaattctc catccggttt gacctccagc
     2041 tcatccacaa tgcccaccag ggcaaggtct acgggtacaa atttgacccc gggcatggct
     2101 aatgccgctt ctctagaacg caccatcacg cacaaatcgc cactccctgc ttgaaccaca
     2161 tccacagcca cctgcagccc tcctaccgga ttgagccgct tatcgagcgg ttggacgatc
     2221 agaaatttca gcccttccag gtcctggtac ttcagcgtgc aaattgcggt gcctttcact
     2281 tttccaaaca gcatgcttaa ccaagcccca catctttcgc tacccgctca atcaccgcat
     2341 ccagcatagc ctcatccacg ctgttgccta atcgcgcttt caccgtctca tgtactttgg
     2401 ctttgatctc cgccacgcgc gaagcttctg cgcacttaca gggagcggaa acggtggcag
     2461 attgcacagg ggtattaaga tacgggcgga tgggcgccgc cggcggagtt tcttccgcct
     2521 gcgcaattct catttccagt ttcctggctt tctcatatgc caggtcggtc atgatcacat
     2581 catctgtgag agtgatggtc atcgttccct gtctgaacag gtcctctaca tcccgttcag
     2641 tgtagaaagt ctttgccatg catatcctcc attttattca gcctgtcctt ccaggttctc
     2701 aagcgcttga atagcggcat tcttggcatt gatgatctct gcttcggaac ccgaaagcca
     2761 catgcgccca aacctgccaa cgctggaaac atgcacaatc ttgatggtag cccgtttttc
     2821 cgcttcgttg caggcatagt tgatatatgc tgccggagcg cactcgagaa ccaacatggt
     2881 ctcaccgggc accagcatac tcccacgtcg gaaacggttt aaaagctgcg cctggtaaga
     2941 gtcaacgcgg gtgatgacct gaaccgaagc gatttgaggt ttaacacgat cttccacttt
     3001 caaacccaat cggtcaagca ccacattccc cgcctcaatc accgcagcct gattcatgga
     3061 atgaacctcg aacatgccaa attcgcgttc cacgatctgg gcgccgggtt tggcttccgt
     3121 cgcttttacc gcaatgtcaa ccgtgcgaaa aacttcattg cccggagcca tttcaatata
     3181 taa
//
LOCUS       DAOSDS010000171         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_11535_length_3010_cov_4.830795, whole genome shotgun sequence.
ACCESSION   DAOSDS010000171 DAOSDS010000000
VERSION     DAOSDS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11535_length_3010_cov_4.830795"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PLL88_11800"
     CDS             <1..807
                     /locus_tag="PLL88_11800"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOJ02288.1"
                     /translation="WRLIVNAPAQGAWNMAVDEAILESTTSHLQPTTLRFYSWNPFCL
                     SLGHGQSVKDIDEESLQKHGWDLVRRPTGGKAILHADEFTYCICAPQNDPHVQGSVLE
                     SYKHLSQGLMGALQNIGLTASSEPHKDPSERNTTQPICFEVPSDYEITLNGKKIIGSA
                     QARKKNGILQHGAIPLFGDIARITDVLQYSSEEERVLSRSQLIAHATTLQEQAGRKIT
                     WKNLAQAFICGFEKALDVKIKPGRLSELERRRAKEILEEKYANPDWTRRL"
     gene            809..1405
                     /locus_tag="PLL88_11805"
     CDS             809..1405
                     /locus_tag="PLL88_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOJ02289.1"
                     /translation="MSNLFNFLLIPMIIFFGLLLERVRLTLRTRSSIRKVKDPSSYRT
                     AIVFGAGLNKSLTPTKALRDRLDKTIQLTDQGQFDRILLSGGQAKETTESVAMRDYLA
                     CQGISSELLLIDEEGISTYDTLKRAKTVFHIQQATLISQPFHLPRAIGIAQMLGMDVI
                     GIAADTRKFKISSILWWHFRELFAWIWSLIKVRLYSGT"
     gene            1449..2543
                     /locus_tag="PLL88_11810"
     CDS             1449..2543
                     /locus_tag="PLL88_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02290.1"
                     /translation="MALINGKGYYIWKIPSCEGGDPDTIALVAQQAGLQHVFIKIANG
                     IYDYNYDSSTKKDLIAPVCDALLAKGIRVWGWHYVFGDLPKDEAKAAIRQINRLPLDG
                     YIIDAEGEYKDKYTPCRIFMNELRAALPDFPMALSSYRYPNYHLSLPWKDFLSKCNYN
                     MPQVYWEQAHNPGEQLLRSLKEFQLIEPFRPIIPTGAAYAAGGWIPTVSDIRAFLDTA
                     VSLNMPAASFWSWDYCRLKLPDIWQAIADYQWPGSTTVLPSVPELWIEYLNKKNIPLL
                     LSLYDEDAVFVTSKQTIQGKNALNGWFSALMSTLFSNATYSLESFSGTAITKKISWKI
                     TTADNRILSIADTIGLQDNKILYHYSSIQY"
     gene            complement(2773..3010)
                     /locus_tag="PLL88_11815"
                     /pseudo
     CDS             complement(2773..3010)
                     /locus_tag="PLL88_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          824 a          739 c          666 g          781 t
ORIGIN      
        1 tggcgattga tcgtgaatgc tccggcacag ggtgcctgga acatggcagt agatgaagct
       61 attcttgaat ctaccaccag ccacttacaa cctaccactt tgcgtttcta ttcgtggaat
      121 cctttctgct tgtcattagg gcatggtcag tcggtcaaag acatcgatga agaaagtttg
      181 caaaaacatg gctgggatct ggtgcgtcga ccaaccggtg gtaaagccat tctgcatgct
      241 gatgaattca cctactgcat ctgcgcaccc cagaacgatc cgcatgtgca aggatcagtc
      301 ttagaaagct ataagcatct ttctcagggg cttatgggag ctttacaaaa cattggattg
      361 acggcaagtt cggaaccgca taaggatcca tctgaaagga atacaaccca accaatttgt
      421 ttcgaagtcc cttccgatta cgagattacg ctcaatggaa aaaagatcat cggcagtgcg
      481 caagcccgca aaaagaatgg cattctccaa cacggagcta ttccgctgtt cggcgatatc
      541 gccagaatca ctgatgtgtt gcaatattcc agcgaggaag agagagttct ttctcgttca
      601 caattgattg cgcacgccac caccttgcag gaacaagcag ggcgaaagat cacctggaaa
      661 aacctggcac aggctttcat ctgtggtttt gagaaagcct tggacgtgaa gatcaaaccc
      721 ggtcgtttga gcgaactgga gaggcgcagg gcaaaagaga tcctggaaga aaaatacgca
      781 aatcctgact ggactcgccg gctatagaat gtccaacctg ttcaattttc tcctcatccc
      841 catgatcatt tttttcggat tactattgga gcgggtccga ttgacgctcc gtacgcgttc
      901 atctattcga aaagtcaaag acccgtcaag ttacagaacc gccatcgtat ttggagccgg
      961 gctcaacaaa tcgctcaccc ctacaaaagc cctgcgcgat cggttagata aaacgattca
     1021 attgacagat cagggtcaat tcgaccgtat tttgctcagt ggagggcaag ccaaagaaac
     1081 taccgaaagc gttgcaatgc gggattacct tgcttgccag ggaatttcat cggaacttct
     1141 attaatcgac gaagaaggga tttccaccta cgatacactg aaaagggcaa aaacagtctt
     1201 ccatattcaa caggcgaccc tcatttcaca accattccac cttccacgtg ctatcggaat
     1261 tgcacaaatg cttggaatgg acgtcatcgg cattgcagcc gacacgcgca agttcaagat
     1321 ttcttcgatt ctttggtggc attttcgaga actctttgcg tggatctggt cgctgatcaa
     1381 agtaagattg tattcaggta cataacttgc aactgtatcc ttgatagaat aagaccaaag
     1441 gatgaaccat ggcattgatc aacggaaaag gatactacat ctggaaaata cccagctgtg
     1501 agggaggcga tcccgacacg atcgctttgg ttgcccagca agccggattg cagcatgtct
     1561 ttatcaagat tgccaatggc atttacgact acaactacga ttcatccacc aaaaaagacc
     1621 tgatagcccc tgtttgtgat gccctgttgg caaaaggtat ccgggtttgg ggctggcatt
     1681 acgtttttgg cgatcttcca aaagatgaag ccaaagcagc catccggcag atcaaccgct
     1741 taccgttaga tggatatatc atcgatgcag aaggggaata taaggacaaa tataccccct
     1801 gtcgaatttt catgaatgaa ttgcgcgccg ctttacctga ttttcccatg gctctcagtt
     1861 cctaccgata ccccaattat catctctccc ttccctggaa ggactttctc agcaaatgca
     1921 attacaacat gccgcaggtt tactgggagc aggcgcacaa tccgggagaa caactgttgc
     1981 gatctttgaa agaattccaa ttgattgaac ccttccgtcc catcatcccg actggcgctg
     2041 cctatgctgc tggaggatgg attcccaccg tatcagacat cagggcattt ctggatacgg
     2101 cagtttcgct gaacatgcct gctgccagtt tttggagttg ggattattgc cgtttgaagc
     2161 ttcctgatat atggcaagcg atcgctgatt accaatggcc cggatcaaca accgttcttc
     2221 cctctgttcc tgagttgtgg atagagtatc tgaataagaa aaatattccc ctgcttctga
     2281 gcctgtatga tgaggatgcg gtttttgtaa cctctaaaca aaccatccag ggaaagaatg
     2341 ctctcaacgg gtggttttct gccttgatgt caaccctttt ttctaatgcc acttattccc
     2401 tggaatcatt ttcgggaaca gcgataacca aaaaaatttc ctggaagata acaaccgcgg
     2461 acaatcgcat tctaagcatt gcagatacga ttggattgca agataacaag atcttgtatc
     2521 attattcttc catccaatat tagaaaaaag ccggggattt ctcctcggct tcacctttcc
     2581 ttattcttca tccaaatcat catccagatc gtcatccaga aaatcaatct cgtctgatga
     2641 aacatcatca tccaaaagat ctgtgtccag gatgtctgaa tccagaatgc catccaacga
     2701 actttcatcg gttggaacct ggatcgtact gacacgcttt gcttctaaag gttcatttcg
     2761 ggccacagcc ccagtcaatt ctacgcgttt ctcggaaaaa cccgttcccg cagggattag
     2821 cttgccgatg atgacatttt ctttcaatcc atacaacgga tctttttcag ccgcaatggc
     2881 tgctcgggaa agttctttga tcgtatgctg gaaggaagca gccgatagga acgattcagt
     2941 gttcaaagat gctttggtaa cacccaataa gatatcgatg taccgcgccg gctttttacc
     3001 ctgcgcgatc
//
LOCUS       DAOSDS010000172         3002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_11568_length_3002_cov_4.170003, whole genome shotgun sequence.
ACCESSION   DAOSDS010000172 DAOSDS010000000
VERSION     DAOSDS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11568_length_3002_cov_4.170003"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PLL88_11820"
     CDS             complement(<1..526)
                     /locus_tag="PLL88_11820"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOJ02291.1"
                     /translation="MNSDQNLYEVARLYYEQNMTQEEIARRLFISRSGISRLIKQARE
                     EGVVEITIHTPYDRMRNLEYEFFRRFHIDEIRIVDTDKRPNPSDFDTVVKLAATYINS
                     ILNEKSVMGLTWGKSIMGAIRELRPTRYLPKMHVTQMTGSIESHTPIIDGPDLVRCVA
                     EAYGCSYHYLMVPFS"
     gene            755..1408
                     /locus_tag="PLL88_11825"
     CDS             755..1408
                     /locus_tag="PLL88_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02292.1"
                     /translation="MPNSKPVLQVALDTLSFEEAFRILGGGLDNVVDIIEVGTLLLLG
                     EGLRAVDILRTIYPQKVLAADFKCVAPHFGTEILKHDPDLVTVLSRAEAQVPRLIHEE
                     ALKRGKGQQVQIELYDQTPTVEDIHTWQQVGITHIIYSRPRSRPGSWGAEDREEIKTL
                     CDLGMQVTATGGVTYEDVDVLAGLPIFAIICGRSMRNAPDPAAEARRIKQKIASLWK"
     gene            1458..2243
                     /locus_tag="PLL88_11830"
     CDS             1458..2243
                     /locus_tag="PLL88_11830"
                     /inference="COORDINATES: protein motif:HMM:NF013916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02293.1"
                     /translation="MKSRYYRLFKEDGKALILAFDHGGNGDIWADPATVISGAAAGGM
                     DGILTNYGVITHFRKEIGRMGTMLRMEVIASGHVKYNPILERPMGSPYSIEDCLRLGV
                     DGVMTMGILGTEMDTANMRYIASVVAACEPYGIITAAEMLPNGFSSKEEDRNLKAMNI
                     ACRAGAELGFDIVKTEFVKPVEDFKKIVANCYADVLALGGARVNNDRQVLENAKQAMQ
                     AGCKGLVIGRNVWGHRNVAGMAAALRRIVHENASVEDALEEIH"
     gene            complement(2308..2739)
                     /locus_tag="PLL88_11835"
     CDS             complement(2308..2739)
                     /locus_tag="PLL88_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02294.1"
                     /translation="MDKIKQLVQTSQLKTNYLSNCHQSKIFFHYDDLSDTFMLLFVPP
                     DKETIVHYLDKYVAILYTPNDYEIVGLQVDYFQSDFIPMYEDLQREWCLSDFVVKDES
                     FWDLTLSVESKKISIALGIIKAKQEVIGKPAEEFEKTLAYA"
BASE COUNT          810 a          685 c          706 g          801 t
ORIGIN      
        1 ccgagaaagg caccatcagg tagtgataac tacagccgta agcttccgcc acgcaccgca
       61 ccagatccgg tccgtcaatg attggggtat ggctctcgat cgacccggtc atctgggtta
      121 cgtgcatctt gggcaaataa cgtgtagggc gcaattcccg aatggctccc atgatggatt
      181 ttccccaagt caagcccatc acgctttttt catttaggat cgaattgatg taggtggcag
      241 ccagtttgac cacggtatca aaatcggaag ggttggggcg tttgtccgta tccacaatgc
      301 ggatctcatc gatatgaaaa cgccggaaaa attcatattc caggttgcgc atgcggtcat
      361 agggagtatg gatggtgatc tccaccaccc cttcttcacg ggcttgctta atcaggcgcg
      421 aaataccgga acgtgagata aacaaacgcc tggcgatctc ctcctgggtc atattctgtt
      481 cgtaatacag gcgggctact tcatacagat tctgatcgct attcatcagg acgttctttc
      541 gctcctcccg agtagctgag aatatttgtg caacactgca cacattccaa gtcattatac
      601 aatgcgcaat aaggaaaaat accagaattg gacaaatgtg caagctaatc atttgtgaaa
      661 accagagagc agcgattgac ccaggtatgc ttagcagtag aatttcacca ggatgatcat
      721 cctgcatcac aaaatttaaa aatggtggat accaatgcct aattcaaaac ccgtgctgca
      781 agtcgcgctg gatacccttt cttttgaaga agcttttcgc atcctgggag ggggattgga
      841 taatgttgtg gatatcattg aggtcggtac gttattgcta cttggcgaag ggttgcgcgc
      901 agtggacatc ttgcgcacca tttaccccca aaaagttctt gcggctgatt tcaaatgcgt
      961 cgctccccac tttggcaccg agatcctgaa acatgatcca gatcttgtta ccgtactttc
     1021 cagagccgaa gcgcaggttc ccaggctgat ccacgaagag gcgctcaagc gcggtaaggg
     1081 gcagcaggtc caaatcgagt tgtacgacca gaccccaacc gttgaggata tccacacctg
     1141 gcagcaggtc ggcatcaccc acatcatcta ttcccgaccg cgttcgcgtc cgggttcctg
     1201 gggggcagaa gatcgcgaag agatcaaaac gctgtgcgat ctaggtatgc aggtcactgc
     1261 cacaggtggg gttacctatg aagacgtgga tgtgctggct ggcttgccga tcttcgccat
     1321 catttgcggg cgctctatgc gcaacgctcc cgaccctgct gcagaagccc gccggatcaa
     1381 gcagaaaata gcttcattgt ggaaataaac ttcattccaa atctacaggt tattaagaat
     1441 agaaaggaat cattatcatg aaaagtagat attatcgttt gttcaaagaa gatggaaaag
     1501 cgctcatcct ggctttcgat cacggcggca acggcgatat ctgggcggat cccgccacgg
     1561 tgatcagcgg ggctgcggct ggaggtatgg atggcattct caccaattac ggcgtaatca
     1621 cccattttcg caaggagatc ggcagaatgg gaaccatgct gcgcatggaa gtgatcgctt
     1681 caggtcatgt gaagtacaat cccatccttg aacgacccat gggcagtccc tatagcattg
     1741 aagattgctt gcgcctgggc gtggatggcg tgatgaccat gggcatcctc ggcacagaaa
     1801 tggacactgc aaacatgcga tatatcgctt cggtggtagc tgcctgtgaa ccgtatggta
     1861 tcatcaccgc tgctgaaatg ttgcccaacg gtttctcttc aaaagaagaa gacagaaacc
     1921 tcaaagccat gaacattgcc tgccgggcag gggctgagct tgggttcgac atcgtcaaga
     1981 cagagttcgt caaaccggtc gaagatttca agaagatcgt tgccaactgc tatgccgatg
     2041 tgctggcatt gggcggtgcc agggtgaaca atgatcgcca agttctggag aatgcgaagc
     2101 aagccatgca agccggttgc aaagggctgg tgatcgggcg caatgtttgg gggcatcgca
     2161 acgttgcagg catggctgct gctttacgcc gtatcgtgca tgaaaatgca tctgttgagg
     2221 atgctcttga ggaaatccat tagcgttttt tgtcaaattg gaggaaaaca aaaagcctgg
     2281 cgaattttgc caggcttttt gtagagacta agcataagca agtgtttttt caaattcttc
     2341 tgcaggcttt ccaattactt cctgctttgc tttaattatc cctaacgcaa tactgatttt
     2401 tttcgattca acgctcaaag tcaaatccca gaaactttcg tctttgacaa caaaatcact
     2461 taagcaccat tctctttgta gatcttcata cattgggata aaatcacttt ggaaataatc
     2521 aacctgcaat ccaacaattt cataatcatt gggagtatat agaatagcaa catatttatc
     2581 aagataatga acaattgttt ctttgtctgg gggaacaaat agaagcataa atgtatctga
     2641 taaatcatca tagtggaaga atattttact ctgatgacaa tttgacaaat aatttgtttt
     2701 tagctgactg gtttgtacta attgtttgat cttgtccata tgacggtttc tccttccttc
     2761 ccatgatctg tcggaaaagc ggaaattaca gtgcctctat tatttgaacc aaatcttact
     2821 acaattttta tgtacttatt gtcatcaaat tgatgaaaca tataatagac atttcgattt
     2881 tctttaactg aatccttgca aataaaatga ggatttcgta ttgctttcat tactattggc
     2941 aaccaagttt actattggca accaagtttc cataaatggt cgtgacccta atatttttcc
     3001 at
//
LOCUS       DAOSDS010000173         3001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_11573_length_3001_cov_4.168364, whole genome shotgun sequence.
ACCESSION   DAOSDS010000173 DAOSDS010000000
VERSION     DAOSDS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11573_length_3001_cov_4.168364"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLL88_11840"
     CDS             <1..732
                     /locus_tag="PLL88_11840"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOJ02295.1"
                     /translation="DVNDEPTIFKALTQQESWLENVTVRDDGSIDFMDGTRTTNGRGT
                     FQLNVIPHRDPSNLPPVKYIFLLNRNFNIIPAVARLKPQQASQYFMLGETTGTSAGGK
                     AEAGKSLRVPGTNPFFTMNDALQANRFHELIQSIPDLEVYLLNTGRVGGDDADPRSRK
                     VKIRHSSAIIEAIFNHSIHWKEDPIFHYDIAAILPDFDDSGLLNPYELYKQQARKEEF
                     EKLAERLQQERETYLRGYPDLHITG"
     gene            1041..1325
                     /locus_tag="PLL88_11845"
     CDS             1041..1325
                     /locus_tag="PLL88_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02296.1"
                     /translation="MNSDIVGLLIQLVSGAAGGNIAGALLKKLNMGPVWNSILGIIGG
                     ILVGWVLPSLGIGNASSLDLTSILSSVLGSGVGGGALMAIVGLIRQAVKK"
     gene            1644..2093
                     /locus_tag="PLL88_11850"
     CDS             1644..2093
                     /locus_tag="PLL88_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02297.1"
                     /translation="MGNPVEDFLKKLSGATEKKATTTSKATAGTSRVAGKSGTGGVSG
                     AAKVSGKAGAGTKTAIPRTKAQTTVVSLESQKAALAKKTNQDIINAAYKVADQLKLPG
                     GPWPLLRAAGWGHLTDKRGDLYKGPAIDEIEALTAEQKAALKKILGL"
     gene            2127..2564
                     /locus_tag="PLL88_11855"
     CDS             2127..2564
                     /locus_tag="PLL88_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02298.1"
                     /translation="MKTRNLFLIISVVAGLLLLAACGSKEEGVTVKSVTLAEGLDENY
                     QPINPTTQFYPSDVIYVSVQVEGAPKSGIMTGQFYYYDQLISEASVDFSTVNEGVIIT
                     VGQDTFVGFNLTPSADWPVDSGYTFKLLVDNAEVGSYAYEVIE"
     gene            complement(2621..2691)
                     /locus_tag="PLL88_11860"
     tRNA            complement(2621..2691)
                     /locus_tag="PLL88_11860"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2657..2659),aa:Gln,seq:ctg)
     gene            complement(2741..>3001)
                     /locus_tag="PLL88_11865"
     CDS             complement(2741..>3001)
                     /locus_tag="PLL88_11865"
                     /inference="COORDINATES: protein motif:HMM:NF039902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02299.1"
                     /translation="IGNRMMKALQSQQDWNTVALDTLVRGLMEKEGKEPRQFLGFLRE
                     AISGQRVTPPLFESMEILGKETVVKRLEENLQFIHENSEDSG"
BASE COUNT          836 a          710 c          712 g          743 t
ORIGIN      
        1 gatgttaacg acgaaccgac catcttcaaa gctctgaccc aacaggaatc ctggttggag
       61 aacgtgaccg ttcgtgacga tggtagtata gacttcatgg acggaacccg cactaccaat
      121 ggcaggggaa cattccaatt gaacgttatt ccccaccgcg atccttcaaa cctcccgccc
      181 gtaaagtata tttttctgct caatcgcaac ttcaacatca ttcctgccgt agcccgtctg
      241 aaaccgcagc aggcatcaca atattttatg ctgggagaga cgacaggtac ctcagccggg
      301 ggcaaagcgg aagccggaaa atcgctgcga gtgccgggca ccaacccctt cttcaccatg
      361 aacgatgcgc tgcaagcaaa tcgttttcac gaactcattc aatcgatccc cgatctggaa
      421 gtgtatctgc tcaataccgg tcgcgtggga ggggacgatg cagatccgcg ctccagaaaa
      481 gtgaagatca ggcatagctc tgccatcatc gaagccatct ttaaccattc catccattgg
      541 aaggaagatc ccatttttca ttacgatatc gccgccatcc tgcccgattt tgatgattcg
      601 ggtctgctga atccgtatga actgtataaa caacaagccc ggaaagaaga attcgagaaa
      661 cttgccgagc gtctccagca ggagcgggaa acatatttgc gtggctaccc tgatctgcac
      721 atcacaggat aattgataac ctcccaatca tcagcccctg cggcatttct cctcactcgc
      781 aatacagcaa cggaatgaag atcgcttctc attgattcgt gatatttgac ctggaacaag
      841 attaagaaaa attattctca gagcatgtat tgtcgcagtg ttggttattc ctgcgcggat
      901 tccgaacaaa atagagagag gggtattgca tgggtgggta taatgtatgt agagagagaa
      961 tgtgtgcaca tgaaaggagg cataggaaaa gtccatatcg tttctcaaaa aaggaattgt
     1021 gatcgaaata aggagtaaaa atgaacagtg atatcgttgg attgctcatt cagttggtca
     1081 gtggcgctgc cggtgggaat atcgcaggtg ctcttctcaa gaaactgaat atgggtccgg
     1141 tctggaattc cattcttggg attatcggcg gtattttggt tggctgggtt cttccatctc
     1201 taggaattgg aaatgcaagt tcactggatc tcacatctat cctttccagc gttctcggca
     1261 gtggagtcgg cggaggtgcg ttaatggcca ttgtcgggct catccgccag gcagtaaaga
     1321 agtaaagttc ttgtgcaaac cggctgctct tcactattaa aaaggcagcc ggttttgtta
     1381 atttctcttt gaattgatcc gaatctaact ttgggggctt gctgtactcg aattcatgga
     1441 taaaaaattt taagagatct ttcgggtcgc ctttcttatc gcactggcgg tgcaactgcc
     1501 gcgatccaga gcggaaacaa ccccgttctt cactcttggc gccaagtatt aacaaaatat
     1561 tacagaaaat gggggccata gaatatatga acttcacctg ctataatggg aataaagtac
     1621 ctcatatttg aaaaggagac gaaatgggta atccagttga agattttcta aagaaattgt
     1681 caggcgctac agagaagaaa gccaccacaa cctcgaaagc gaccgcagga acctccagag
     1741 tggctgggaa aagcggaacg gggggagtga gcggcgcagc caaggtgagc ggaaaagccg
     1801 gcgctgggac aaaaacagcc atcccgagaa caaaagcaca aaccactgtt gtgagcctcg
     1861 agagccaaaa agctgctctt gccaagaaga ccaatcaaga catcatcaac gcagcttata
     1921 aggtagcaga ccagcttaaa ctgcccggag gaccatggcc tttgttgcgt gccgccgggt
     1981 ggggacacct caccgacaaa cgtggggatc tgtataaagg tcctgccatt gatgagatcg
     2041 aggcactaac cgccgaacaa aaagcggcat tgaagaaaat tttagggctt tgatcccttg
     2101 caaacctatt tggaaaaggt ataaccatga agacaagaaa tctctttttg attatcagcg
     2161 tagtggctgg tctactcctt ttggctgcct gcggcagcaa agaagaaggg gttaccgtaa
     2221 aaagtgtcac actagccgaa ggtctggatg agaattacca acctatcaac cccaccacgc
     2281 aattctaccc gtcagatgtc atttacgttt cagttcaggt ggaaggcgct ccaaaatctg
     2341 gaatcatgac cggacaattc tactattacg atcaattgat cagcgaagcg tcggtcgatt
     2401 tctcaaccgt caatgaaggt gtgatcatca cagttggtca agatacattc gttggattca
     2461 atcttacccc ctctgcagac tggccagtag acagcggtta cacgttcaag ctgctggtag
     2521 ataatgctga ggttggctca tacgcctacg aagtgatcga ataaatcgaa agttgatgca
     2581 aaaaagacct tccaaaaaaa gaaggtcttt tttgtttttg ctgggggcaa aggattcgaa
     2641 cctctacaaa cagatccaga gtctgctgtc ctgccattag acgagccccc aatgtgctcg
     2701 cgcgcattat tttaccaccg atccgcatat tttggaattt ttacccggag tcctcagaat
     2761 tctcgtggat gaattgcaaa ttctcttcca aacgcttgac gacggtttct tttcctaaaa
     2821 tttccattga ttcaaagagt ggtggggtga cacgttgccc gctgatcgcc tcgcgcaaga
     2881 accccagaaa ttgcctgggt tcttttcctt ctttttccat cagccctctt acaagggtat
     2941 ccaatgccac tgtattccaa tcttgttgag attgcaacgc tttcatcatc cgattgccaa
     3001 t
//
LOCUS       DAOSDS010000174         2940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_11881_length_2940_cov_4.358406, whole genome shotgun sequence.
ACCESSION   DAOSDS010000174 DAOSDS010000000
VERSION     DAOSDS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11881_length_2940_cov_4.358406"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PLL88_11870"
     CDS             complement(<1..342)
                     /locus_tag="PLL88_11870"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOJ02300.1"
                     /translation="MSSASENVVFIDKNTTLQPAIQTWKLYLRDQNKSLYTVKAFEGD
                     LKLLSAYLPADKSVGSVTTNDLQHFLDWVNNGRGKNIPCSPKSLSRRITTIKSFFNWL
                     FATGCIAADPAE"
     gene            complement(405..1433)
                     /locus_tag="PLL88_11875"
     CDS             complement(405..1433)
                     /locus_tag="PLL88_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOJ02301.1"
                     /translation="MKTSIMRHGSAILAGLLIVFLTAGCSLVGSALGFSSEATPQVRL
                     LADKSSEVMEPTPFQPYAYTQTANAVPVVITDTLVPNEGYSKPDGQINLLLLGSDWRA
                     DSGYRTDVIMLISFYTKEEKISIVSFPRDLWVSIPGVGEQRINTAQAYGGFALTQRTF
                     EYNFGTSVDHYMMTNFSGFMSIIDTLGGIEINAAQNLYDRCDLSYSHGGYCSIGPGTA
                     SLDSEMALWYVRSRYTSNDFYRMDRAREVIMGILKKIVSLDGVARAGDIYNTLSSSVV
                     TDLSLSDIVPLVTLAAKVMNDSSKITMYEIGEAQVTSYVMPSSGANVLLPNYDAIWNV
                     LKTALFTP"
     gene            complement(1435..>2940)
                     /locus_tag="PLL88_11880"
     CDS             complement(1435..>2940)
                     /locus_tag="PLL88_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02302.1"
                     /translation="YQSSLLNFNGVHFSTSPNTQEPSESVFSHAIIFPSMHTMADLLT
                     SILNSLSSPPGLVLQSILMLFLMVSAISLINAEKQHIHKTDLRQARTTFVFVLLLNGM
                     AIALQITSIVGLISLSDHIRNAADQLVWSCNLILVGWLWIAPLHRSRFGVFKHILLCT
                     ALAFFIIEAFQPIETLFAPLKITIPYLLIWRGFEFVAGIFLFFICLFYSGKMRFVSIA
                     FIVLQILGLGADQVFIVTSQFSQTFAQFLAFLFSVKLYLALRFDLSEPETAMQSPPVI
                     PSDNLSVMPNAVTTKSWLQTALENDASLLPFTLCKALAYTFHADACLIVQAEKSQTQL
                     KLICGYATENQKQILPRDLLLDDRMVAQKRSALLHGTETFPTWIKTLAQSIHRSNSRS
                     AWYVPLTGFEKKYFFVMLSHREQWGDDHMAYLKKVMPELVQILHNYFREEHILLPENK
                     PLQTVSNPFLELMRTEVDQTKNIQRVEAELQLALEEYNRIRKILEERGIGQ"
BASE COUNT          813 a          640 c          758 g          729 t
ORIGIN      
        1 ttcggcgggg tcagcagcga tacatccagt ggcaaagagc cagttgaaga aagatttgat
       61 ggtggtaatg cgacggctca ggcttttcgg agagcagggg atattctttc cgcggccgtt
      121 gttcacccag tccaggaaat gctgcaagtc gttggtggtg acgctgccaa cggacttatc
      181 ggcgggcaaa taggcagaga gcaatttcaa atcaccctca aaagccttta cggtgtacaa
      241 agatttattc tggtcgcgca gatacagctt ccaggtctga atggctggct gtagtgtggt
      301 atttttatcg atgaacacca cattctcact ggcagatgac atacagtctc cttattgcgt
      361 atagatatag aaagtgtatc tgttttatat catttcgtca agcatcaggg ggtaaacaag
      421 gcggttttca aaacattcca gatggcgtcg taattaggca gcaagacgtt ggcgccgctg
      481 gatggcatga catacgatgt cacctgtgcc tcaccgatct catacatggt gatcttggaa
      541 gagtcgttca ttaccttggc tgccagcgtg accagcggga caatgtcgga aagggaaagg
      601 tcagtgacaa cgctgctgga taaggtgttg taaatatctc ccgctcgtgc aacgccatca
      661 aggctgacga tctttttcag gattcccatg atgacttcac gcgcgcggtc catgcgatag
      721 aagtcgttgc tggtataacg ggaacgcaca taccagagcg ccatttcact gtccaacgat
      781 gcggtcccgg gaccgatcga gcaataccca ccgtgagaat aggaaaggtc gcagcgatca
      841 tagagattct gcgctgcgtt gatctcaatc cctcccaagg tatcgatgat actcatgaaa
      901 ccgctgaaat tggtcatcat gtagtgatcc acggaagtgc cgaagttgta ttcgaaagtt
      961 ctttgggtta aggcgaaccc gccatacgcc tgggctgtgt tgatgcgctg ttctcccaca
     1021 ccggggatgg aaacccacag gtcgcgaggg aatgacacaa tgctgatctt ctcttctttg
     1081 gtataaaagg agatcaacat gatgacgtcg gtgcggtatc cgctgtcagc ccgccaatcg
     1141 gacccaagga gcagaagatt gatctgccca tcaggtttgg aatacccctc attgggcacg
     1201 agcgtatcgg tgatcaccac agggaccgca ttcgcggtct gggtatacgc ataaggttga
     1261 aatggggtag gttccatgac ctcgctgctt ttgtcagcca gaagacgaac ttgtggtgtg
     1321 gcttctgatg agaatccaag ggctgatcca accagcgagc agcccgcagt aagaaacacg
     1381 ataagcagac cagcaagaat tgctgatcca tgacgcatga tggatgtctt cacgctattg
     1441 tcctattcca cgttcttcca gaattttacg gatacgatta tattcctcca gtgccaactg
     1501 aagttccgct tctacccgtt gaatgttttt tgtttgatcg acctctgttc tcatcagttc
     1561 aaggaatggg ttagaaacag tttgcaatgg tttgttctcc ggaagaagta tgtgttcttc
     1621 acgaaaataa ttatgcaaga tttgtaccag ttcaggcatg acctttttca aatatgccat
     1681 gtggtcatcc ccccactgtt ccctgtgaga cagcatgacg aagaaatatt tcttttcaaa
     1741 gccagtaagg ggaacatacc aggcggaccg tgaatttgat cggtgaatgc tttgagccag
     1801 cgtttttatc caggttggga aggtttcagt cccgtgcaaa agggcagacc ttttttgtgc
     1861 gaccatccta tcgtctaaaa gcagatcccg tggaaggatc tgtttttggt tttcagtcgc
     1921 atacccgcaa atgagcttaa gctgcgtttg agatttttcc gcttgaacga tcaaacaggc
     1981 atcagcatgg aatgtgtatg ccagtgcctt acataaggta aaaggtaaaa gggaggcatc
     2041 attttcaagt gcggtttgaa gccagctttt cgtggtgact gcgttaggca tcactgagag
     2101 attgtcggaa ggaatgaccg gcgggctctg catcgctgtt tctggttctg aaaggtcaaa
     2161 ccgcagagca agatataact tcaccgagaa taaaaatgcc aggaattgtg caaaggtttg
     2221 ggaaaactgg gaggtcacaa tgaaaacctg atcggcacct agtcccagga tttgcaatac
     2281 gatgaatgca atgcttacaa accgcatttt tccggaataa aagagacaga tgaaaaacaa
     2341 aaagatacca gctacgaact caaacccccg ccatatcaaa agatatggaa tggtaatttt
     2401 aaggggcgcg aagagcgtct caatgggttg gaacgcttcg atgatgaaaa aagcaagcgc
     2461 ggtacatagc agaatgtgtt tgaaaacgcc gaagcgagag cggtgaagag gtgcaatcca
     2521 caaccacccg accaagatca agttgcaact ccaaaccagt tggtcagcgg cgttgcggat
     2581 gtggtcagac aaggaaatca accccacaat gcttgtaatc tgcagtgcta ttgccattcc
     2641 attcagcaga agaacaaaca caaaagtagt tcttgcctga cgcaggtctg ttttgtggat
     2701 gtgttgtttt tcggcgttga tcagggagat cgcgcttacc ataaggaaaa gcatgagaat
     2761 agattgcaag accaatccgg gaggtgaact cagagagtta agaattgaag ttaacaaatc
     2821 agccatcgtg tgcatagatg gaaaaattat agcatgggaa aaaacactct cagacggttc
     2881 ttgggtgttg ggagaagttg aaaagtgaac accattgaaa ttcaaaagtg aactttgata
//
LOCUS       DAOSDS010000175         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_12067_length_2907_cov_4.161992, whole genome shotgun sequence.
ACCESSION   DAOSDS010000175 DAOSDS010000000
VERSION     DAOSDS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12067_length_2907_cov_4.161992"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PLL88_11885"
     CDS             complement(<1..228)
                     /locus_tag="PLL88_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOJ02303.1"
                     /translation="MPTKRIEIVQDILNVNDQIAEQTNELLHSKHIFSVNIMASPGAG
                     KTSVILRTLQELSPEIHCAVLEGDTAPVTIDA"
     gene            complement(242..586)
                     /gene="hypA"
                     /locus_tag="PLL88_11890"
     CDS             complement(242..586)
                     /gene="hypA"
                     /locus_tag="PLL88_11890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOJ02304.1"
                     /translation="MHELAVTEEILRKAEQIAEQEQADKVTEIFLEIGRLSGIVDESV
                     QFYWDEISKNTVCAGAVLHFKITPASLICKDCGKEYELDHDLIPCPACSGMNIKITGG
                     DEFILTSIEIEK"
     gene            complement(654..1154)
                     /locus_tag="PLL88_11895"
     CDS             complement(654..1154)
                     /locus_tag="PLL88_11895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOJ02305.1"
                     /translation="MMRKKFIILGYGNPDRGDDGVAYHLLNELVQKCSTKDDDLIEFQ
                     EAGILALAPQVDLWYNLQLVPEIAQELANYEQAIFLDAHTAEIPEPILVKKITPGYTN
                     SPFTHHLTPASCLELAERLYQHAPAATLITVRGYSFDFSRDLSPETRLLADQALTIIL
                     EMINGN"
     gene            complement(1243..2667)
                     /locus_tag="PLL88_11900"
     CDS             complement(1243..2667)
                     /locus_tag="PLL88_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOJ02306.1"
                     /translation="MTQKITIEPVTRIEGHAKITINIKEDGSVEHAYLHINEFRGFEK
                     FCEGRMVFEMPLITPRICGICPVSHHLASAKAADAALGVEPPRPASLLRELMHMGQVI
                     QSHGMHFFELAGPDLLLGWDADPAIRNVVGIIQANPALALQAVNLRKYGQEIIKTVGG
                     RKIHPTFAVPGGVNKALTGQERDSILAGLTTAIATTKTGLDLFKGWAAQHADEIAEFA
                     VFQSGYMGLVTPENGLELYDGNIRLVDKSGKQLELFPASHYLENIAEHVEDWSYLKFP
                     YYRKMGYPAGVYRVGPLGRLNTCEKIDTPLAQQEFVEYHKINDGKPIENTLYFHYARL
                     IETLFAVEKVGVLLDDPDILSQDILNTRQNYQGEGIGVIEAPRGTLFHHYWVDKNGQM
                     EKVNLIVATGNNNWAMCEAVDSVAKRYVQGPDITEGMLNRVEAAVRAHDPCLSCSTHA
                     VGEMPMIVEVRDLQGNLLNVVKRG"
     gene            complement(2686..>2907)
                     /locus_tag="PLL88_11905"
     CDS             complement(2686..>2907)
                     /locus_tag="PLL88_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOJ02307.1"
                     /translation="RAYVETESTDSNGLIPSSPELATMTRVRAIHEVVPVDIYIPCCP
                     PDADIIFYALSELAQGRIPELKNDTLMWN"
BASE COUNT          685 a          747 c          713 g          762 t
ORIGIN      
        1 ggcgtcaatg gtgactgggg ctgtatctcc ttcaagcacg gcgcaatgaa tttcaggtga
       61 aagttcctgc agggtgcgca ggatcacgct ggtttttcct gcaccggggg aagccatgat
      121 attaaccgag aaaatgtgct tggagtgcag cagttcgtta gtctgttctg cgatctgatc
      181 attaacgttc aggatatcct ggacgatctc aatacgtttg gttggcatac ttctccttct
      241 ttcatttttc tatttcaatg cttgttagaa tgaactcatc tccgcctgtg atcttgatgt
      301 tcataccgct gcacgcaggg cagggaatga gatcgtggtc gagctcatat tcctttccac
      361 aatccttaca aatgagactg gcgggagtga ttttgaagtg caggaccgcc cctgcacaaa
      421 ctgtattctt gctgatctca tcccagtaga actgcaccga ttcatcaacg ataccggata
      481 gtctgccaat ctccagaaag atctctgtga ctttatcggc ttgttcctgt tctgcgatct
      541 gctctgcttt gcgcagtatt tcttccgtga cggctagttc atgcatactg gcattataat
      601 cgaaaccgca tatttatgta gatatttatg gtaaaaataa taaaaaatat agcctaattc
      661 ccgttgatca tttcaagaat gatcgtcaac gcctggtcag ccaacaatct cgtttcaggg
      721 gaaagatctc gcgaaaaatc aaaggaatat ccgcgcacgg tgatgagtgt ggcggcgggc
      781 gcatgctggt agagtctctc agccagttcc aggcaactgg cgggggtaag gtggtgggtg
      841 aagggagaat ttgtgtatcc tggcgttatc ttttttacca aaatcggctc agggatttcc
      901 gcagtatgcg catccagaaa aattgcctgc tcatagttcg ctaactcttg cgctatttca
      961 ggaacgagtt gcaggttgta ccacaggtca acctgcggag caagagccag gatgccggct
     1021 tcctgaaatt cgatgagatc atcatccttg gtactgcatt tctggacgag ctcattcagc
     1081 agatgataag ccacgccgtc atcaccgcga tctggattgc catatcccag aatgataaat
     1141 ttttttctca tcattccatt ctaaccccta caagaaagaa cggtgccgca aacccatatc
     1201 ctgtgcagca ccgtccttga acaagcaagc tcaacctgtt gatcagcctc gtttcaccac
     1261 attcaaaaga ttgccctgca ggtcacgaac ttccacgatc atcggcattt ctcccacagc
     1321 gtgggtggag cagcttaagc atggatcgtg agcgcggacg gcggcttcca ctctgttcaa
     1381 cataccttcg gtgatgtcag gtccttggac ataacgtttg gcaacgctat ccaccgcctc
     1441 gcacatcgcc caattgttgt tgccggtggc aacgatcaga ttgaccttct ccatttggcc
     1501 attcttatca acccagtaat gatggaaaag cgtcccgcga ggtgcttcga tcaccccgat
     1561 cccttcaccc tgataatttt gacgggtgtt gaggatatcc tgggagagga tatcgggatc
     1621 gtcaagcagc acacctacct tttcaaccgc aaagagcgtt tcgatcaggc gggcataatg
     1681 gaagtaaagc gtgttctcaa tcggtttgcc atcattgatc ttgtggtatt ctacaaattc
     1741 ctgctgcgcc agaggggtat caatcttttc acaggtgttc aacctgccca acggaccaac
     1801 gcgatacaca ccggccgggt aacccatctt gcgataatag gggaatttca ggtaagacca
     1861 gtcttctacg tgctcggcga tgttttcgag ataatgagaa gccgggaata attccagttg
     1921 ttttccgctt ttatccacca ggcggatatt accgtcgtac aattccagac cgttctctgg
     1981 agtgaccaga cccatataac cagattggaa gaccgcaaac tcagcgatct catcagcatg
     2041 ttgggcagcc cagcctttga acaaatccag cccggtttta gtagtggcga tggcggttgt
     2101 taaccctgcc agaatgctat cgcgttcctg cccggtcagc gctttgttca cgccccctgg
     2161 cacggcaaag gttgggtgga tcttgcgacc gccaacggtc ttaatgatct cctgaccgta
     2221 cttgcgcaga ttgaccgcct gcaatgccag cgctggattg gcttgaatga tcccgaccac
     2281 attacgaatc gcagggtcag catcccaacc gagcagcagg tcaggaccgg ctaattcaaa
     2341 gaagtgcatg ccatggctct gaatgacctg acccatgtgc atcagttcac gcaacagcga
     2401 ggcggggcgg ggtggttcca cgcccaaggc agcatccgct gctttggctg atgccaggtg
     2461 atggcttacg ggacagatac cgcaaatgcg gggcgtgatg agcggcattt caaaaaccat
     2521 gcgcccttca cagaactttt cgaagccacg gaactcgtta atgtgcagat aggcatgttc
     2581 cacactgcca tcctctttga tattaatggt gatcttggcg tgcccttcaa tcctggtgac
     2641 cggttctata gtaatttttt gtgtcatttc tctcttgcct ctctgctagt tccacatcaa
     2701 cgtatcgttc tttagctcag gtatgcgccc ctgcgccagt tcgctcaagg cgtaaaagat
     2761 gatatcggca tcaggtggac aacaaggaat atagatatcg acgggtacga cctcatgaat
     2821 ggcacgcacg cgggtcattg tggctaattc cgggctgctg gggatgagcc cattggaatc
     2881 tgtgctttcc gtttccacat acgcgcg
//
LOCUS       DAOSDS010000176         2859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_12324_length_2859_cov_4.519971, whole genome shotgun sequence.
ACCESSION   DAOSDS010000176 DAOSDS010000000
VERSION     DAOSDS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12324_length_2859_cov_4.519971"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..2225
                     /locus_tag="PLL88_11910"
     CDS             156..2225
                     /locus_tag="PLL88_11910"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001960.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOJ02308.1"
                     /translation="MASNRWLYLFGLLLFGGLIIAAVAFGFSLWSVPILVGLFAIYLA
                     GIFVSRIIFRKVNSQEMLDVSEDIHAAARVYMRRQVRTILLTVPFMALVVGFFMGWRE
                     TIAFVLGVCTSISAGYLGMSISVRTNVQTADQAEESFSSAFRTAVFGGGVMGLLVTGL
                     SLLVLTILFLIYQDPDVLIGFGVGGSLAALFGQIGGGIFTKSADVGADLVGKVEEKIP
                     EDDPRNAAVIADLVGDNVGDCAGRGADLFQTFSDDIITGSVVCATLVSIYGSDVIYLP
                     ILLQCVGIVSSAIGILATRQWSKEQKPTSIFNVGLWISSILSSLGALGLSYWLIDDLS
                     IGVAVILGVATMLITAATTRYYAGINHTPVNKIAAASHRGAALTLMTGTAFGLRSPII
                     SLLTIITAIMLAFKIAGGTILAILGVNIGTDLLIGIIMASDAFGPIVDNAAGIAKIGK
                     AKRKVFKSLSHLDTIGNTLKATTKSYAMASGTVTAFVLFATFFTITGNTVLDLITPYS
                     IGFIFVGISLPFLMSSLVIGSTAKAAELMVDAVRDQFKQNPAIIEGKTKPNYTHCVDI
                     ATKNALREMLLPGGIAFLLPIVVGFLFGGAALGALLIGAVSSSALLGAYFNNTGTAFD
                     NAKKTIEEDHTLDGSLAHASAVVGDTYGDPLKDVAGPSLLIFMKLIGMTSLLIAPILN
                     TTTVIFQ"
     gene            2407..>2859
                     /locus_tag="PLL88_11915"
     CDS             2407..>2859
                     /locus_tag="PLL88_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOJ02309.1"
                     /translation="MALRVRLASPETIKSWSYGEVTKPETINYRRLRPEKDGLFCEAI
                     FGPTRDYQCYCGKYKNPRYKGIVCDKCGVEVTRSSVRRERMGHIELATPVAHIWYSRR
                     VPSYLGSLLNISRRNLDRVLYFAQYIVTYVDEDSKQKALNRIEEEVSVK"
BASE COUNT          659 a          619 c          730 g          851 t
ORIGIN      
        1 tctattagga cattttaact ttgccagttt aggactttac tactttgctt ctacacacgt
       61 tttttctagc atattttccc atcggaatat catatataat agctatctat ttgctcttct
      121 gtcatttact gtttcatcta taaggagaag cattgatggc ttcgaatcgt tggttatatc
      181 tgttcggtct cttattgttt gggggtttga tcatcgcagc agtcgctttc ggatttagtt
      241 tatggtcagt tcccatcctg gttgggttat ttgcgattta tctggcaggc atttttgtta
      301 gccgcattat ctttcgcaag gttaactctc aagaaatgct ggatgttagc gaggatatcc
      361 atgcggctgc cagagtgtac atgcgccggc aggtgcgcac gattctgttg accgtccctt
      421 tcatggctct cgtggtgggg ttttttatgg gatggagaga aacgatcgct tttgtgttag
      481 gcgtttgcac ctccatttct gcaggatacc tgggaatgtc cattagtgtt cgcaccaatg
      541 tgcaaactgc tgatcaagcg gaggaatctt tctccagcgc tttccggacg gccgtttttg
      601 gcgggggagt gatggggttg ctggtgaccg gattgagttt gctggtactg acaatccttt
      661 ttctgatcta ccaggatcct gatgtgctga tcggctttgg ggtgggcgga tcgttggcgg
      721 cgttatttgg gcagattggt ggcggtattt ttaccaaatc agcggatgtt ggggcagatc
      781 tggttggaaa ggtggaagag aagatccccg aggatgatcc gcgtaacgct gcggttattg
      841 ctgacctggt tggggacaat gtgggcgatt gtgccgggcg tggtgcagat ctctttcaga
      901 ctttcagtga tgatatcatc acaggcagcg tggtatgcgc cactctggtc tcaatttacg
      961 ggagtgacgt aatctacctt ccaatattgt tgcaatgtgt gggaatcgtc tcttcggcta
     1021 tcggcatact ggcaaccaga cagtggtcga aggaacaaaa accaaccagc attttcaacg
     1081 ttgggttgtg gatcagctcc atcctcagct cgcttggcgc gcttgggtta tcctattggt
     1141 tgatcgatga cctttctatc ggggtggcag tgatattggg tgtggcgacc atgttgatca
     1201 ccgcagcaac tacgcgctat tatgctggca tcaatcacac gccggtaaat aaaattgcgg
     1261 cagcctcgca ccgcggggca gcccttacgc tgatgaccgg cactgctttt ggattgcgaa
     1321 gcccgattat cagcttgctc acgattatca cagcgatcat gctggctttc aaaatagccg
     1381 gaggaaccat tctggcgatt ctgggggtga acattgggac cgatctgttg attggcatca
     1441 tcatggcatc cgatgccttt ggaccgatcg tggataacgc agccggtatt gccaagatcg
     1501 gcaaagccaa aaggaaggtt ttcaaatctc tttctcatct tgatacgatt ggaaacacgc
     1561 tcaaggcaac taccaagtct tatgccatgg catcggggac ggtgactgcc ttcgtattgt
     1621 ttgccacatt cttcaccatc accgggaaca cggttctcga tctgatcacc ccatattcca
     1681 tcggattcat ttttgtaggt atttctttgc ctttcctcat gtcttcgctt gtgatcggct
     1741 ctacggcgaa agctgcagaa ttgatggtgg atgcggtacg cgaccagttc aaacagaatc
     1801 cggcgattat agaagggaaa accaaaccga actataccca ctgcgtggat attgccacca
     1861 agaatgcgtt gcgggaaatg ctccttccgg gtggcatcgc attccttttg ccgattgtgg
     1921 tcggattttt gtttggcggc gctgctttag gggcgctctt gatcggcgct gtttcttctt
     1981 cagccctgtt gggtgcctat tttaacaaca ccggcacggc ttttgataac gcgaaaaaaa
     2041 ccatcgaaga ggatcacact ttggatggct cccttgccca tgcgtcagcg gtggtagggg
     2101 atacctatgg cgatcctttg aaggatgttg ccggtccttc tttattaata ttcatgaaat
     2161 tgattggcat gacctccttg ttaattgcac cgatcctgaa cactaccact gtgatctttc
     2221 aataaggctt gtatgtttag agagggaaga tttctttata atacgtcttg caaatcccta
     2281 taaagtagat attaataatg atatttcgtg ttataatgtt tgttttgtca aacaactgtg
     2341 tatgtattta aattaatttg agcggtattt gtgtgaggag gctcataagt ggaaacaaaa
     2401 agtctgatgg cgctccgagt aagacttgca tctcccgaaa cgataaaaag ttggtcttat
     2461 ggggaagtaa cgaaacctga aacaatcaac taccgtcgct tacgccctga aaaagatggg
     2521 cttttctgtg aagcgatctt tggaccaacc cgagattacc aatgctattg cggcaaatat
     2581 aaaaacccga gatataaagg gattgtatgt gataaatgcg gtgtcgaagt aacccgttct
     2641 tctgtgcgtc gcgaacgcat ggggcacatt gagctggcaa cgcctgttgc ccatatctgg
     2701 tatagccgaa gggttccttc ttacctgggt tcccttctca acatttcgcg cagaaacctg
     2761 gatcgcgttt tgtattttgc gcaatatatt gtcacatatg ttgatgaaga ctccaaacaa
     2821 aaagcgctga atcgcataga agaagaggta agcgtaaaa
//
LOCUS       DAOSDS010000177         2824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_12521_length_2824_cov_3.535572, whole genome shotgun sequence.
ACCESSION   DAOSDS010000177 DAOSDS010000000
VERSION     DAOSDS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12521_length_2824_cov_3.535572"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="PLL88_11920"
     CDS             <1..625
                     /locus_tag="PLL88_11920"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOJ02310.1"
                     /translation="FMDYLFFEQGAMYGWVFHYMPIGRSYTLDLMPSIQQRQWMWQRS
                     WELIKEKKLFLADFWNHGTLVEGCLAAGRSSGGGYLYINWDGKVMPCVFVPYSPVNLN
                     DIYQQGKTIADAWEESFFERIRMWQAAYLKRDGQRGNLLNPCFIRDHHDVMRRIVADT
                     EPDPENEAAHEALLDGAYEQGMLAYGKAYQELSDPIWQQHYLKHDAE"
     gene            731..1618
                     /locus_tag="PLL88_11925"
     CDS             731..1618
                     /locus_tag="PLL88_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOJ02311.1"
                     /translation="MPYTLFTDSCCDMPQNYYAENGIHVLLLDYLISGHTFTDNMSSP
                     EEFHRFYQQVRAGEMPTTVQINTSQFIAAFEPVLRQGEDVLYIGFSSALSGTYNSARL
                     ACEDLRERYPERKITVIDSVSASMGQGLLVHQVAQLKKQGESFDAVAQWVEDNKCNLH
                     HWFTVDDLHHLHRGGRVSATSAVAGTMLDIKPVLHVDNKGRLIPQTKTRGRKKALRTL
                     VDYMESLALDIGKTPVFISHGDCLEDALTLAEMIHERFDVAVDMIHFIGPIIGSHSGP
                     GTVALFFMGKNREAHLPKA"
     gene            1913..>2824
                     /gene="pncB"
                     /locus_tag="PLL88_11930"
     CDS             1913..>2824
                     /gene="pncB"
                     /locus_tag="PLL88_11930"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR01513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0019357 - nicotinate nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0019358 - nicotinate nucleotide salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOJ02312.1"
                     /translation="MSKKHKELSTGILFTDLYQLTMAQIYYRNGMRETPAQFDYFFRS
                     YPDYGSHQAGYCINAGLEWFIDWLQEAKFGQDEISYLSGLKNTQGNALFAPDFLEWLA
                     SDQPFSRISMRAIPEGRVVHPNVPIAIVEGPIIQAQLIESALLNYLNYQTLIATKAAR
                     IHESCHGQMVMEFGLRRAQERAANAGARAAIIGGADSTSNVGISSMLGTQPSGTHAHS
                     MIQAFIALGGTELDAFRAYAAEYPDNCILLVDTISTLESGIPNAIKVFEELKASGHSP
                     IGIRLDSGDLAYFSIQAAKMLDEAGFQD"
BASE COUNT          713 a          708 c          742 g          661 t
ORIGIN      
        1 attcatggat tacctgttct ttgagcaggg agctatgtat gggtgggtat tccattacat
       61 gccgatcggt cgatcctaca cgctggacct gatgcccagc atccagcaac gccagtggat
      121 gtggcagcgg tcatgggaac tgatcaaaga gaaaaagctc tttctggctg acttctggaa
      181 tcacggaacg ctggtggaag ggtgcttggc ggctgggcgc agcagcggag gaggctatct
      241 atacatcaat tgggatggca aagtgatgcc gtgcgtcttc gtgccctatt cgccggtcaa
      301 cctcaatgac atctaccagc agggcaaaac catcgccgat gcctgggaag aatccttctt
      361 cgagcggatt cgcatgtggc aggctgcata tctcaagcgg gatgggcagc gcggcaacct
      421 gttgaacccc tgcttcattc gcgatcatca cgatgttatg cgccggatcg ttgccgatac
      481 ggaaccggac cctgagaatg aagcggctca tgaagcgctg ctagacggtg catatgagca
      541 aggcatgctc gcctatggga aagcctacca ggagctgagc gatccgattt ggcaacaaca
      601 ctatctgaaa catgacgcag agtgaaaagt gaagtagaaa actttgaccg tgtaagaaat
      661 cgtcaacaac ctgagaaagc atgaaatttc cccggctgtt ttatgagtca ccaacaagga
      721 ggcagttttg atgccctaca cgctgtttac agattcctgc tgcgatatgc cgcaaaatta
      781 ctatgcggaa aacggtatcc atgtgctttt gctcgattat ctgatcagcg gtcatacctt
      841 tacagataat atgagctctc cagaagaatt tcatcgtttt tatcagcagg tgcgcgcagg
      901 cgaaatgccc accacagtgc agattaacac cagccaattc attgccgcct tcgagcctgt
      961 gcttcggcag ggagaggatg ttctgtacat cggcttctcc tccgccctga gcgggaccta
     1021 taattctgcc cgattggcgt gcgaggatct acgcgaaaga tatcctgaac gcaaaatcac
     1081 cgtaatcgat tctgtcagcg catccatggg acagggcttg ctggtacatc aggtggcgca
     1141 attgaaaaag caaggggaat cctttgatgc ggttgcccaa tgggttgagg ataataagtg
     1201 taacctgcac cactggttta cggtggacga tttgcatcac ttgcaccgcg gtgggcgagt
     1261 atctgctacg tcagccgtgg caggaacgat gcttgacatc aagcccgtgt tgcatgtgga
     1321 taataagggt cgcctgattc ctcagacgaa gacacgcggg cgcaaaaagg cactcagaac
     1381 gctggtggat tatatggaat ccttggcatt ggatattggc aaaaccccag tctttataag
     1441 ccacggcgac tgtctggagg atgctttgac gctggcagaa atgatccacg agcgttttga
     1501 tgtagctgtc gatatgattc atttcatcgg gccgatcatc ggctctcatt ccggaccggg
     1561 tacggtggcg ctgttcttta tgggaaaaaa ccgggaggca catctgccca aggcatagcg
     1621 cgtccatagc agatcatcgc ttgttggaaa tcgctggtcg ctcggcatct taaatctgtc
     1681 cgtggctatt gactcggcgg cgatgacggc aggaactggt acagttctgt ttttgccgcc
     1741 tattgtcagc gggtttgcag ccgcttctaa caaaaaagca cccatatttg gcatcagcgc
     1801 gatctgtttg gacagccggg gttccatgag cttgcacaag ctgcaggaag agggaactga
     1861 gtcccttttc ttgttttata atccaggtat cacttaacga ccgagaaatg gaatgagtaa
     1921 aaaacacaaa gaactatcta cgggaatcct gtttaccgac ctgtaccaac tgaccatggc
     1981 acagatttat tatcgcaacg gcatgcggga aacgcccgcc cagttcgatt atttctttcg
     2041 cagttatccc gattacggct cacaccaagc cggttattgc atcaacgcgg gcttggaatg
     2101 gttcatcgac tggctgcaag aagccaagtt tggacaagat gagatatcgt acttatccgg
     2161 attgaagaac acgcaaggca atgcactttt cgcacccgat ttcttagaat ggctcgcaag
     2221 cgatcaacca ttctcccgca tttccatgcg cgccatcccc gagggacgtg tggtacaccc
     2281 caatgttccc atcgcaatag tagaaggacc gatcatccag gctcaattga tcgaatcagc
     2341 tctcttgaat tacttaaact atcagaccct catcgcaacc aaagccgctc gcattcacga
     2401 aagctgtcat ggtcaaatgg tcatggaatt tggtctgcgt cgcgcacaag aaagggctgc
     2461 caacgcaggc gcccgggcag ccataatcgg cggtgcagat agcacatcca atgtgggcat
     2521 cagcagcatg ctaggcacac aacccagcgg cacgcatgcc cacagcatga tccaggcatt
     2581 catcgccctg ggcggaacag aactggacgc ttttcgtgcg tatgccgctg aatatccaga
     2641 caattgcatc ctactggtgg atacgatcag cactctcgag agtggcattc ccaatgccat
     2701 taaagtgttc gaagaattga aagccagtgg acatagccct atcggtattc gcctggattc
     2761 aggggatttg gcatatttca gcatccaggc agccaagatg ttggatgaag cgggatttca
     2821 agat
//
LOCUS       DAOSDS010000178         2807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_12619_length_2807_cov_3.729651, whole genome shotgun sequence.
ACCESSION   DAOSDS010000178 DAOSDS010000000
VERSION     DAOSDS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12619_length_2807_cov_3.729651"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..859
                     /locus_tag="PLL88_11935"
     CDS             401..859
                     /locus_tag="PLL88_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02313.1"
                     /translation="MVYDLRNHQIPTPLTVGGMVGAGVYALFNGLWAPVLLMIALTHV
                     SDFNPREKRLAFAFTLSAFSAIFQPSAALICLLILVVWVLWEFGVLGGADVKLIITAA
                     LVLGNPIFLIPISIVGGVQGVIASLQKKREIPFVVSIFCGTLLFVLYPYF"
     gene            882..1028
                     /locus_tag="PLL88_11940"
     CDS             882..1028
                     /locus_tag="PLL88_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02314.1"
                     /translation="MRFLKDNRGIESTEVALIIAVVVLVAYGAYRLLGQNIAAIVTDI
                     AGKI"
     gene            1109..1519
                     /locus_tag="PLL88_11945"
     CDS             1109..1519
                     /locus_tag="PLL88_11945"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HOJ02315.1"
                     /translation="MRFIKDTRGMELLEAAITTPIAIMVMLAIVNLGMLVYAQQAVQA
                     AARHGARMGSVAQQCPACYAMSGARDAIAQAGILENTSVSVLAPGGSAGSILKIQVSG
                     SVPNFMAPLTSLFPGLPGQTFTVRADSTFRQEGW"
     gene            1521..1955
                     /locus_tag="PLL88_11950"
     CDS             1521..1955
                     /locus_tag="PLL88_11950"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOJ02316.1"
                     /translation="MLWRDRRGYSMTFWAVFIGLVMIPALALAIELGRYFYAISEVAK
                     AADAAAVGASAEINQQVFQDTGSLVPTSRTWGNAQAYVTSNTASLSAKGVHAFVTDIQ
                     VSGGDNTVRVSVSADLSILFPSVVPKVLVTQTGVAKLRVFTH"
     gene            2155..2679
                     /locus_tag="PLL88_11955"
     CDS             2155..2679
                     /locus_tag="PLL88_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02317.1"
                     /translation="MINDVVLEGIVVRDPWKFMDDLFFRLVIYRDSDLPAKKLDLERD
                     AGDYINVRVNGGANGLIHIRRGMRLRVHGFLQSRDFRESLEEFINKARKSKNCTDLAV
                     DIKACDLKQNQVFIDRNVVETVARRIIVLDAAAPNEKKARSIERVTTGKHTEADNDSS
                     EVESASGEIDATAE"
BASE COUNT          732 a          575 c          704 g          796 t
ORIGIN      
        1 agatgttcta taataatagg agattttact caaaaataat gagatttcat ttttaccaac
       61 tattgaaaaa atgtaataac ttcgagagat tgtgagctca ttatctgtga tgagctaaaa
      121 agaagagaga tgtataaagg ttctacaaga aggcggaatc aaggattcct tgttcgtaac
      181 aagcagtgag gaaagagtca ttttcccttg accacctagg ataagaatgt ttttttgata
      241 atattaagtg tagacaatct ttgatcaatc gttggctgga cagaaccttc tgggtgcaga
      301 tgtgtccagc tttatttttt aagcacttgc actaatgcag aatggatagg agcgctttct
      361 gattgccgct ttcgccgttt tagccctgtt ttttacctgt atggtctacg atctccgcaa
      421 ccaccagatc cccacgccat taaccgttgg cgggatggtt ggtgcaggtg tgtatgccct
      481 tttcaatggt ttatgggctc cagtattgtt gatgattgca ctgacacacg tatcagattt
      541 caacccccga gaaaaacgtc tggcattcgc ctttacgttg tccgcatttt ccgcaatttt
      601 tcaaccttca gcggcgttga tttgccttct catccttgtc gtgtgggtgc tttgggaatt
      661 tggtgttttg ggaggtgcgg atgtcaaact gatcattact gctgccctgg ttcttggaaa
      721 tccaattttt ctgatcccga tatcaattgt cggtggagtg cagggcgtaa tcgctagcct
      781 tcagaaaaag agggaaattc cttttgtcgt atcaattttt tgtggaactt tgttgttcgt
      841 tctctatcca tatttctaaa gaaggaagga ggtgattggc attgcgtttc ctaaaggata
      901 accggggaat cgagtcgact gaggtcgcat tgatcattgc agtagttgtt ctggtggctt
      961 atggtgcata ccggttgctg ggccaaaata tcgctgccat cgtgacagat attgctggaa
     1021 aaatctagtc aaatcaatgg tctgccagca ttgtcccctt cggggatgtt ccaaaaactg
     1081 gcagatcaat tattcctcaa ggaggattat gaggtttatt aaagacactc gtgggatgga
     1141 attgctagag gctgcaatta caactccaat tgcgattatg gtcatgttgg cgattgttaa
     1201 cctggggatg ttagtctatg cccaacaggc agtgcaagcc gcagccaggc atggtgcgcg
     1261 tatgggaagt gtggcgcagc aatgcccagc ctgttatgcc atgagtggag caagagacgc
     1321 aatcgcccag gctgggattc tggaaaatac ttcggtcagt gtcttggctc ctggaggaag
     1381 tgccggcagc attttaaaga tccaagtcag cggcagtgta cccaacttta tggctcctct
     1441 gaccagcctg ttccctggat tgccagggca gacgtttact gtccgtgccg attctacatt
     1501 tcggcaagaa ggctggtaat atgctgtggc gagatcggcg tggctattca atgactttct
     1561 gggcggtatt cattgggttg gtaatgattc ctgccctggc attggctatt gaactcggac
     1621 ggtattttta tgccatttca gaagtggcaa aggcagcaga cgctgcggca gttggcgctt
     1681 ccgctgagat caatcagcaa gtttttcaag ataccggaag tcttgttccc acttcaagaa
     1741 cctgggggaa cgctcaggct tatgtgactt caaatacagc cagcttatcc gctaaagggg
     1801 tccatgcatt tgttacagac attcaagtca gtgggggaga taacacggta cgggtctctg
     1861 tgtcagcaga tttatctatc ctgtttccat cagtagttcc caaggtgctt gtcactcaaa
     1921 ccggggtggc caaactacgt gtcttcacac attgatctat tatttcccat aaaggagcgt
     1981 ggcattattg aaaagaaatt gaccatctag gataagcccc ttgtaattga tatactctga
     2041 ttgacaactc aaaacttctt catctatcgt tggccggaca caacccactg ggatctgtcc
     2101 ggcattttct ttttaaaccc aagatggcga aggtttcata aacaaggagg tcagatgata
     2161 aacgatgtag ttctagaagg aatcgtggta cgggatccat ggaaatttat ggatgacctg
     2221 ttcttccggt tggtgatcta tcgagacagt gatctacctg ccaagaaatt ggatttggaa
     2281 cgtgatgctg gtgattacat caacgtccgg gtaaatggtg gagctaatgg gctgatccac
     2341 atccgacgcg gcatgcgatt gcgagtgcat ggtttcctcc agtctcggga tttccgcgaa
     2401 agcttggaag agtttatcaa caaagcacgg aaatccaaaa actgtactga cctggctgtg
     2461 gatatcaaag catgcgattt gaaacaaaac caggtattca ttgaccgaaa cgtggtagag
     2521 acagtcgcca ggcggattat tgtgttggat gctgccgcac cgaacgagaa aaaagccaga
     2581 tcaattgaga gagttacgac tggcaagcac actgaagcgg ataatgattc aagtgaggtg
     2641 gaatctgctt ctggagaaat tgacgcaact gctgaataac tcatcctatc aacaaaccga
     2701 aggtgagcat gggtaatcct ctttttggat tacctgcaaa aggcctacgc gtgtaaaaaa
     2761 atagagagat ggatgagttc agacctgaag cgaattctta atacttc
//
LOCUS       DAOSDS010000179         2732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_13061_length_2732_cov_3.487486, whole genome shotgun sequence.
ACCESSION   DAOSDS010000179 DAOSDS010000000
VERSION     DAOSDS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13061_length_2732_cov_3.487486"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PLL88_11960"
     CDS             <1..1162
                     /locus_tag="PLL88_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HOJ02318.1"
                     /translation="QYMQANQQDFEEKEQLRKARIRIGSNGITTDFILEYLERSLPVV
                     LDFANFSEAELYLKKEKTTSLLQAFHYGELKESFWRGETHELGKSALGKIAQMVEPRA
                     IDLRSEQLPEIKAMLVQHGFVRLYCFPIRAESGTLGVLCLAGRADQMMDEDMEYLLME
                     TASLIGAVIFHERQSLESKRSIVMEERERIGMDLHDGIIQSLYGVGLSLQNAILQLDQ
                     GKDASEEQIGVALDSINEAIRDIRAYILDLRPRQLRDKNLYMGIESLVREFRANTFLD
                     VDLEEDLKDIDVLPDENAEILFHICQEALANIAKHAKATRVNIRIWETPQRVMIKVSD
                     DGVGFDLAKINQRLGHGLSNMTTRAENAGGGIEVISIRHQGTTVLAWVPSGS"
     gene            1215..1952
                     /locus_tag="PLL88_11965"
     CDS             1215..1952
                     /locus_tag="PLL88_11965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ02319.1"
                     /translation="MGNQPISSRAALRGEPSYVWRAGQERRLDMIRNYARERLQGMIL
                     ENGCGVGEYLQRLSEQAKLAVGLDVDLERVLETHQKGLTVQMAVGEALPHAAGTFDLI
                     LSNEVIEHVQDDRQAVAEMIRVLKPGGRLMLFCPNRGYPFETHGIYRKGQYHFGNKLF
                     VNYLPDRWRNKLAPHVRVYRKRDLEKLFIGLPIRFVYHTVIFGGYDNIIRRSPALGKL
                     LRGFLQFLEKTPLRALGLSHFWVVEKS"
BASE COUNT          680 a          647 c          768 g          637 t
ORIGIN      
        1 gcagtatatg caagcaaacc aacaagattt tgaagaaaaa gagcagctgc gcaaggcgcg
       61 catccgtatt ggtagcaacg gaatcaccac tgatttcatt ctggagtatc tggagcgttc
      121 tttgccggtg gtgctggact tcgccaactt ttcagaagcc gagctctacc tgaaaaagga
      181 aaaaaccacc agtttattac aggcattcca ttacggcgaa ttgaaagaat ccttctggcg
      241 cggtgaaacg cacgaattgg gcaaaagtgc cctgggtaag atcgcacaga tggttgaacc
      301 gcgggcaatt gatctgcgca gcgagcagct gccggagatc aaagccatgc tggtgcagca
      361 tggctttgtg cgcctatact gcttccccat ccgggctgag agcggcacgc tgggtgtgtt
      421 gtgcctggca ggcagagcag atcagatgat ggatgaggat atggaatacc tcttgatgga
      481 gaccgcttcg ttgatcggag cggttatttt tcacgagcgc cagtccctcg aatccaagcg
      541 ctcgatcgtg atggaagagc gcgagcgtat cggcatggat ctgcacgatg gtatcatcca
      601 gtcgttgtat ggggtggggc tttcgctaca gaatgccatc ctgcagttgg atcagggcaa
      661 ggacgccagc gaagagcaga tcggggtggc gctggattcc atcaacgaag ccatccgcga
      721 tatccgggct tacatcctgg atctgcgccc gcgccaactg cgcgacaaga acctctacat
      781 gggcatcgaa agcctggtgc gcgagttccg tgccaatacc tttttggatg tggatctgga
      841 ggaagatttg aaggacatag atgtgctgcc ggatgagaat gccgagatcc tgttccatat
      901 ctgccaggaa gccctggcaa atatcgcaaa acatgccaaa gccaccaggg tcaacatccg
      961 catttgggaa acaccccagc gtgtaatgat caaggtgagt gacgatgggg taggttttga
     1021 cctggcgaag atcaaccaac gcctggggca tggactttcc aatatgacca cgcgcgccga
     1081 aaatgcaggt ggcggcatcg aggtgatttc catccgccac cagggcacca ccgtgctggc
     1141 ctgggtgccg agcggctcat aagataaaat tccattacaa taacaactta ccttttcgat
     1201 cgaggattat aactatggga aatcaaccaa tatccagccg agctgctctg cgcggcgaac
     1261 cctcctacgt gtggcgcgcc gggcaggaac gacgcctgga tatgatccgc aattatgcca
     1321 gggagcgcct gcagggtatg atcctggaaa atggctgcgg ggtgggcgaa tacctgcagc
     1381 gcttgagcga acaagcgaag ctggcggtgg gattggatgt ggacctggag cgtgtgctcg
     1441 aaacccatca gaaagggctg acggtgcaga tggcagtggg tgaagcgttg ccgcatgccg
     1501 caggcacctt tgatctgata ctcagtaatg aagtgatcga acatgtgcag gatgaccgcc
     1561 aggcggtggc ggaaatgata cgcgtgctga agccgggggg acgcctgatg cttttctgtc
     1621 ccaaccgcgg ctaccccttt gagacacatg gcatctaccg caagggtcaa tatcatttcg
     1681 gcaataaatt gttcgttaat tacctgccgg atcgctggcg caacaagctg gcgccgcacg
     1741 tgcgcgtgta ccgtaaacgc gatctggaaa aattatttat cggcttgccg atacgctttg
     1801 tgtaccacac agtaatcttc gggggctacg ataacatcat ccgccgcagc ccggcgctgg
     1861 gtaagctgct gcgcggtttt ctgcagtttc tggaaaaaac gccgctgcgg gcgttgggtc
     1921 tttcacactt ctgggtggtg gaaaagagct aagtttaatg gtgtgttaga agtgtatcct
     1981 atctgcttat ttcaacgtca tccagaggga gtgtaacgac cgatggatct ctccatgtcg
     2041 aggttaaaaa tgcagaaaag tcgcaaagca atttgtcagt caataaacca atctttcgca
     2101 aggtctttcc actcgggatt cagagaatta ataagagcaa atttctttgc cctgctcatg
     2161 cccttgatgg ctttttcacg gagaatagcg gcggaaggga acggaaattc ttcatagtaa
     2221 accagtctgg tgatgtggtg cgactggtga aaccgggcag agttttgtgt ttatgctcga
     2281 aaacacgtct ttccaggttg ccaatcacac cggtgtaaag gatgcgggaa taatttgcca
     2341 tgatgtatac gtagaaagtt ctcgtgttga ccccaaactg tattttatat tcagttattc
     2401 aaaatgggat gtgtatggta tggtaatgga tcttaacctg ctggttggtt attcatacaa
     2461 atggtgagat gcttcgcttc gctctgcatg acaccccctc cttgcctccc cgccagcaat
     2521 aggggaggaa aaaactactc gcctgttctc tcaatgtcat ccagagggag cgcagcgacc
     2581 gtgggatctc tccatgtcaa agtcataagc atggaaaagt cgcaatgaag caggttagtc
     2641 aatagaccaa agattacccg tgggttaaga gaccaatctg gcagtaactc gacttacgta
     2701 ctggtttgag cttttcatgg tgagatgctt gt
//
LOCUS       DAOSDS010000180         2676 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_13385_length_2676_cov_3.595193, whole genome shotgun sequence.
ACCESSION   DAOSDS010000180 DAOSDS010000000
VERSION     DAOSDS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13385_length_2676_cov_3.595193"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLL88_11970"
     CDS             <1..1077
                     /locus_tag="PLL88_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02320.1"
                     /translation="ANLPEKGDEDHPFGPGFITTRVQQICVIKNYHGFLRVLQAVFPS
                     QKDGYFFTACEKIDQMIDLQTRYLSGKLFCRTREGIPVVFPEVQVQYRFVRKLPIRQT
                     PSSSPNSEEEPVRNYILQKGALSDQFLIRHYTDLVFSRVMRGFPLEMLNGIPPMEAET
                     ITQEKVAEHFTKVRKFKINALSKRWRNSPMLAVSVNYIRTRRLRASTFRSHRKRLQPA
                     KKGIFAFSSHMPDWNTASVDPIPTIVKQVEACLAKELQNFSIQLESLHISTWQPAENG
                     VNQKIQQTHQKGAELFSKQRALNARQIILASKNASLISLRTPDASVRPEPPVDLTLKS
                     QLLLKKKQAYRMPEEQPANDKNGV"
     gene            1126..2031
                     /locus_tag="PLL88_11975"
     CDS             1126..2031
                     /locus_tag="PLL88_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOJ02321.1"
                     /translation="MIPMQFNPEIKAHLAKNDIVALESTVITHGLPYPQNETLALDME
                     KEVRDHGCVPATIGLVNGELIIGLNRTQIHDLSKGRSNLRKISQRDFGIALAKKMNGG
                     TTVAGTILASVQAGIHVFATGGIGGVHRGSIFDVSADLQVLSQYPMIVVCAGAKSILD
                     LPATLEKLETLGIPVLGYQTDDFPAFYSKSSGLAVDFRVDNPEEVIEIAHSHWEMGIR
                     SSILVVVPPPDAVALPYEEVEKEIQKAVHDAEEHGIRGAKLTPFLLERMSQLTAGKSM
                     HTNLALLTNNARVAALIARAYAQKR"
     gene            complement(2035..>2676)
                     /locus_tag="PLL88_11980"
     CDS             complement(2035..>2676)
                     /locus_tag="PLL88_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02322.1"
                     /translation="KVKQKSALSVIILGISILLIAFLLATFAEPAIYTVYVPSPTATL
                     TPTVTLTPTVTLTPTVTPVPTATPVPLYTATPFLSVIISSQFISQTTPDPEAVFSPLV
                     FARNIDSALQPVEASDVFTPPLDVIYATFSYDRMTRNAQWTALWFREGELICIESIPW
                     NGASGGYGYTECRLSEDQWQPGNYSVQIFVGETWKQTGTFQVSGGSDIEVSEE"
BASE COUNT          711 a          703 c          682 g          580 t
ORIGIN      
        1 gctaatctac cggagaaagg tgacgaagat catccctttg gacccgggtt cattactacg
       61 cgcgtgcaac agatttgtgt catcaagaat taccatggtt ttctgcgcgt cctgcaagcg
      121 gtgtttccca gccaaaagga tggctacttc ttcaccgctt gcgagaagat agatcagatg
      181 attgaccttc aaacacgata tctgagcgga aagctattct gccgcacacg cgaaggcatc
      241 ccggtagttt ttcctgaggt tcaggttcaa taccggttcg tgcgaaaact gcccatacgc
      301 caaacgccat cctcctcacc caactcggaa gaggagccag tcaggaacta catcctgcaa
      361 aaaggcgccc tttccgacca gttcctcatt cgccattaca ctgatcttgt tttttcccgc
      421 gtcatgcgtg gctttcccct ggagatgctc aatggcatcc ctccaatgga agcagagaca
      481 ataacgcaag aaaaagtcgc tgaacatttt acgaaagtgc gcaagttcaa aatcaacgct
      541 ctgagcaaac ggtggagaaa ttcaccaatg ctggcggtgt cagtgaacta tattcggacc
      601 aggcgcttgc gggcatccac cttccgcagc catcgaaaac gcctccagcc cgccaaaaag
      661 ggcatcttcg ctttctcatc tcacatgcca gattggaaca ccgccagcgt ggaccctatc
      721 cccacgatcg taaaacaagt cgaagcttgc ctggcaaagg aattgcagaa tttcagcatc
      781 cagcttgaat ctttgcatat ctccacctgg cagcctgctg agaacggcgt gaaccagaag
      841 atacaacaaa cccatcaaaa aggggcggaa ctgttcagca agcagcgcgc gctgaacgcc
      901 cggcagatca tcctcgcatc aaaaaatgcc agcctgattt ctctacgaac gccagatgca
      961 tctgtgcgac cggaaccgcc cgtggatttg accctgaaaa gccaacttct gctgaaaaag
     1021 aaacaagcct acagaatgcc ggaagaacag ccagcgaatg acaaaaatgg agtatagaat
     1081 gagagcagca aaacaaaatc actaccatca agaagggaga attccatgat accgatgcag
     1141 ttcaaccctg aaatcaaggc tcaccttgct aaaaatgaca tcgtagcgct tgagtcaacc
     1201 gtcatcacac acgggctacc ctatccgcaa aatgaaaccc tcgctctaga tatggaaaag
     1261 gaggtccgcg accatggttg tgttccagcc accatcggat tggtgaatgg tgaattaatc
     1321 attgggttga acagaacgca aatccatgat ctttccaagg gtcgcagcaa cctgcgcaag
     1381 atcagccagc gcgattttgg tatcgcattg gctaaaaaga tgaacggcgg aacaacggtt
     1441 gccggtacca ttctggcttc cgtacaggct ggcattcacg tgtttgccac cggcggtatc
     1501 gggggagtgc accgcggttc gatattcgac gtttccgctg acctgcaggt gctatcccaa
     1561 tatcctatga tcgttgtgtg tgcaggagcc aaatccatcc tggatctgcc cgctacgctg
     1621 gagaagctgg aaaccctggg catcccggta ttgggttacc agaccgatga ttttccagct
     1681 ttctactcga agtccagcgg tctggctgtg gattttcgcg tggataatcc tgaagaggtc
     1741 attgagatcg cccattcaca ctgggagatg ggtattcgct cctcgattct ggtcgtggtt
     1801 cccccgcctg atgctgtggc actaccttat gaagaggtgg aaaaagagat acaaaaagct
     1861 gttcacgacg cagaagaaca tggcattcgg ggggcaaagc tcaccccttt cttgcttgaa
     1921 cgaatgagtc agcttaccgc cgggaagagc atgcacacca acctggcttt acttaccaac
     1981 aatgccaggg tggcggctct gatcgccagg gcatacgcgc agaagagata gcaattattc
     2041 ttcggagact tctatgtcac tgcctcccga cacctgaaac gtgccggttt gtttccacgt
     2101 ttcccccacg aagatctgca ccgaataatt tcctggttgc cactggtctt cactcaaccg
     2161 gcattcagtg tacccatagc cgcccgaagc gccgttccag gggatagact ctatacaaat
     2221 cagctccccc tccctgaacc acagggcggt ccattgcgca ttgcgagtca tacgatcata
     2281 cgaaaaagtg gcatagatca catcaagcgg aggggtgaaa acgtcgcttg cttcaacggg
     2341 ctgcagtgcg ctatcgatgt tcctggcaaa gacaagaggg ctgaagacag cctccggatc
     2401 gggcgttgtt tgcgagatga actgactgct gatgatgacg gacagaaaag gagtggcggt
     2461 gtacagcgga acgggggtag ccgttggaac tggcgtgacg gtgggggtca atgttacggt
     2521 cggggtcaaa gtgacagtgg gcgtcaacgt ggcagtggga gagggaacgt agaccgtgta
     2581 gatagctggt tctgcaaagg ttgcaagcag aaaagcaatc aataaaatgg agattcccaa
     2641 aatgatgacc gacagcgcag atttctgttt gacttt
//
LOCUS       DAOSDS010000181         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_14362_length_2531_cov_4.602989, whole genome shotgun sequence.
ACCESSION   DAOSDS010000181 DAOSDS010000000
VERSION     DAOSDS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14362_length_2531_cov_4.602989"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..885
                     /locus_tag="PLL88_11985"
     CDS             160..885
                     /locus_tag="PLL88_11985"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HOJ02323.1"
                     /translation="MKSILQDVLDHLTKDYPVKSVGVYSHSILVTSCFSGIASTVLGN
                     QPHGEELVPEAGYLLEKSALQLAASCTSQNSLEAGIGLAAINSLLEIPQGNVRSGNVV
                     QILEKQGKGKKIALIGHFPFISRLKQTAKDVWVIDFNPAPGEFHPDDAPRLLPQADII
                     AMTANTLITKMAEDYLTQCNPSALKIMLGPSTPMLATLFEHGLDILAGVRVTDPDLVQ
                     RFLSQGASFSQLQGVEKITLFKN"
     gene            930..1023
                     /locus_tag="PLL88_11990"
     tRNA            930..1023
                     /locus_tag="PLL88_11990"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:964..966,aa:Sec,seq:tca)
     gene            1097..2296
                     /locus_tag="PLL88_11995"
     CDS             1097..2296
                     /locus_tag="PLL88_11995"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOJ02324.1"
                     /translation="MENEQLKYYDIVAQEMTISDVVYGPTQTNPYIAKFSGQLKNPDS
                     ERSYATVEAALKPLKLTPVFRLEGDRQVILLVPEIAKPKKTNPRTNLILFILTVISVL
                     FTGGLYGLETALPQNAWQAILVILKNGWPFAVSLLAILGTHEFGHYFMGKKHGVDVTL
                     PYFIPFPFNTFGTMGAFINMRSLPKNKKQLFDIAAAGPLSGLIVSIIVLLIGLNLSEV
                     YALPATLEAGTGLQMEGNSLLYLFLKYITFGKLLPAPANTSGILLIIHWFKYFFTGSP
                     IPLGGLDVMLHPVAWAGWAGLFVTMLNLIPVGQLDGGHIFQALFGTKNSKRILPFIIA
                     TLVLLGFFWNVWWFWAILILFMGRSYAEPLDQITQLDTKRKWLGVVMMIVFVITFIPV
                     PVTILYG"
     gene            complement(2321..>2531)
                     /locus_tag="PLL88_12000"
     CDS             complement(2321..>2531)
                     /locus_tag="PLL88_12000"
                     /inference="COORDINATES: protein motif:HMM:NF025397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02325.1"
                     /translation="KPDCKVISTTQEHGVIAMDPLSFSQYRVGDVIQVFPVHSCLAAH
                     LLGEYLDIHTGEKISTLVVKKDFSP"
BASE COUNT          667 a          611 c          554 g          699 t
ORIGIN      
        1 catcgcaggt tgggttctgg gaatactgtt gggcttgtgt atgctctggt tgcaaccctt
       61 cttctatcaa atttttccat ggatcttctt tcgatagatc acccttcgaa aggtaattca
      121 aaacaatttt gaactacaat caaaggggag aatttttcga tgaaatcaat tcttcaagat
      181 gttctagatc atctcacaaa agattatccc gtaaagagtg tcggtgttta ctctcactcc
      241 attctggtga ccagctgttt cagcggtata gcctccacgg ttctcggcaa tcaaccacat
      301 ggcgaggaat tggtacctga agcgggatat ttgcttgaaa aaagcgcatt gcagttagcc
      361 gcatcgtgca catcacagaa ttcccttgaa gctggaatcg gtttggctgc catcaattct
      421 ctactcgaaa ttcctcaagg caacgtccga tctggaaatg tcgtacaaat ccttgaaaaa
      481 cagggcaaag gaaaaaagat cgcgctgatc ggtcattttc ctttcatctc acgtttaaaa
      541 cagaccgcga aagacgtttg ggtgatcgac ttcaatccgg cacctggtga atttcaccca
      601 gatgacgcac ctcgcctttt gccccaggca gatatcatag ccatgaccgc caacaccttg
      661 atcacgaaaa tggctgaaga ttacctcacc caatgcaacc cttctgcttt aaaaatcatg
      721 ctcggtccaa gcacacccat gttagctacc ctgtttgagc atggactgga catcctggca
      781 ggcgttcgcg ttacagaccc ggatttggtt caacgcttcc tttcacaagg cgcttcattt
      841 tcccaactac aaggcgttga aaagatcaca ttgttcaaaa actagtgctt catctttaca
      901 atccatgtgg tctgggatat cctttaaccg ggaatggtag ggtcccggtg gatccctcgg
      961 tcttcaaaac cggtgacggg ctgttatgca ggtcgtggtg ggttcgactc ccatccattc
     1021 ccgcctttta agataattct tatgtagcat ttcccccaac ctaagtacaa tacatccagc
     1081 aaacaaggaa aaatccatgg aaaatgagca actgaaatat tatgatatcg tcgcacaaga
     1141 aatgacgatc agcgatgtgg tttacggtcc cacgcaaacc aacccctata tagcaaaatt
     1201 cagcgggcaa ttgaaaaacc cggatagcga gcgttcgtat gcaacggtag aagctgcact
     1261 gaagccactg aagttaaccc ccgtctttcg gctagaaggg gaccgacaag tgatcctgtt
     1321 ggtgcccgag attgccaaac cgaaaaaaac gaatccacgc accaacctga tccttttcat
     1381 attgacagtg atcagcgttc ttttcacggg tggtctatat gggctggaaa cagcactgcc
     1441 acaaaatgcc tggcaggcga tcctggtcat tttgaaaaat ggctggccgt ttgccgtctc
     1501 attgctggca attttgggta cgcatgagtt cggtcattac tttatgggca agaaacatgg
     1561 agtggatgta acccttccct atttcatccc atttccgttc aacaccttcg gtaccatggg
     1621 tgctttcatt aatatgcgct cgctacccaa aaataaaaaa caattattcg atattgcggc
     1681 agcaggacca ttaagcggct tgatcgtttc gattattgtt ttattgatcg gtttgaatct
     1741 atcagaagtg tatgccttac cagcaacact tgaggcaggc acaggactgc aaatggaagg
     1801 aaactctctt ctatatttat ttctgaaata tatcactttc ggaaaactcc tccctgcccc
     1861 agccaatacc agtggcatcc ttctgatcat tcactggttc aagtatttct tcactggcag
     1921 tccaattccc ctgggagggt tggatgtgat gttgcatccg gtagcctggg ctggttgggc
     1981 tggtcttttt gtaaccatgt tgaacctcat cccggtgggg cagttagatg gcggacatat
     2041 ctttcaagcg ttgtttggca caaagaactc gaagcgcatt ctccctttca tcatagcaac
     2101 gcttgtatta ttgggtttct tctggaatgt atggtggttt tgggcaatac tcatcctgtt
     2161 catgggcaga agctatgcag aacctctaga tcagattaca caactggata caaagcgtaa
     2221 atggctcgga gtcgtcatga tgattgtttt tgtcatcact ttcatcccag ttccggtcac
     2281 cattttgtac ggttgataga aagacgactt tgtaaaaagg ttatggggaa aaatcctttt
     2341 tcaccaccag cgtcgagatt ttttcaccgg tgtggatgtc taagtattct cccagcaaat
     2401 gcgcagccag acaggagtga acaggaaaca cctgaatcac gtctcccacc cgatattgcg
     2461 aaaaactcag cggatccatt gcaataaccc catgttcctg cgtggtcgat atcaccttgc
     2521 aatcgggttt g
//
LOCUS       DAOSDS010000182         2481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_14758_length_2481_cov_3.608821, whole genome shotgun sequence.
ACCESSION   DAOSDS010000182 DAOSDS010000000
VERSION     DAOSDS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14758_length_2481_cov_3.608821"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLL88_12005"
     CDS             <1..344
                     /locus_tag="PLL88_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOJ02326.1"
                     /translation="SDAYYQNLKDQFGDSLTLSEEFRWEWVSIPHFYHTPFYVYAYAF
                     GQLLVLSLYKQFKTEGDAFKPRYIHMLSTGGSKAPMQILDEAGMDVRRSAFWQGGFDL
                     IDNMVTQLEEL"
     gene            complement(391..768)
                     /gene="ssb"
                     /locus_tag="PLL88_12010"
     CDS             complement(391..768)
                     /gene="ssb"
                     /locus_tag="PLL88_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ02327.1"
                     /translation="MPSLNRVQLIGRLGKDPENRSTPKGSAYTTFSLAVDRRWKDKTG
                     ETHQDTDWFNVEAWGKLGELCGKFLTKGKLVYLEGRLRTRRYEHEGSTRYFTTVVLGA
                     MQILEKKTKEPELVEESEEEVAD"
     gene            856..1494
                     /locus_tag="PLL88_12015"
     CDS             856..1494
                     /locus_tag="PLL88_12015"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOJ02328.1"
                     /translation="MYIAIEGVIGVGKTSLARLLQPSFKANLLLEIFEENPFLQEFYQ
                     DRARYAFQTQIFFLLSRYRQQRNNVPVLLQTGKNLLTDYTFEKDALFADINITGDELD
                     MYYSVHTALAEKIPIPDLTIYLKASTEVLMQRIATRDRSYERSMESGYINDLNGAYDA
                     FFSKSGENKKVLTIDTNDIDFVHNLSDLQMIDNRIRQTLHMPPFQPSLPIMK"
     gene            1507..>2481
                     /locus_tag="PLL88_12020"
     CDS             1507..>2481
                     /locus_tag="PLL88_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02329.1"
                     /translation="MRVTRNLLLETARNHVKSQTLNNPDIACVFLVGSLLSDNPFIGE
                     NTDIDLVFIHNTEPAAPREVIPMGDEFNLDILHFPRRIFNQPKTLREDPWLGCLFCEK
                     PFVLYDSYHFYDFLRAGVYSNFFSPTYAYARSQYFYIRARKRWMDLHQAEQGKNFSVS
                     FVFAYLQTLYDAANTIACLTGKPIPERRFLTTLEAITTYLGRPGLASGMRDLFAPTNY
                     DLIDWQDLHQQLTIIFSILSDKSYCPPQYAPARVNYYLGAASYYQVERFDESIWILLW
                     VWTNIMQMLPKRSPEVRGWKDFCEQLNFSRDSIPLKLQQLDIYLDAVDE"
BASE COUNT          636 a          620 c          495 g          730 t
ORIGIN      
        1 tatcggatgc ctattatcag aacttaaaag accagtttgg tgattctctg accctcagtg
       61 aagagttccg ctgggaatgg gtttccatcc cgcatttcta ccataccccc ttctatgtgt
      121 atgcgtatgc gtttggtcag ctgctggttc tttcacttta caaacagttc aaaacagaag
      181 gcgatgcttt caaaccgcgt tatattcaca tgttatccac aggcggttca aaagccccaa
      241 tgcaaatact ggacgaagcc ggtatggatg ttcgtcggtc tgctttctgg cagggtggtt
      301 ttgatctgat cgataatatg gtcacccagc tcgaagagct ttagaaatga aaaaagaaaa
      361 ggctgtcgat aattggcagc ctttatactt ttaatctgcg acttcttctt ccgattcctc
      421 gactaattct ggttctttgg tcttcttctc aagaatctgc atggcgccca atacaacagt
      481 ggtgaaatag cgcgtgctgc cctcgtgttc ataacgacgg gtacgcagtc ttccttccag
      541 atagaccagt ttacccttgg tgagaaattt tccgcaaagc tcgcccagtt ttccccaggc
      601 ctctacattg aaccaatcag tgtcctggtg tgtttcgccg gttttatctt tccagcgccg
      661 gtctaccgcc agagaaaaag tggtatacgc gctgcctttg ggagtgctgc gattttcggg
      721 atccttcccc aacctaccaa ttaattgaac tcgatttagt gatggcattc tatccctcca
      781 tttttcataa atcaatgtta tataatagtt tagattattt aatcccgttg tcaatatttt
      841 tctttaggag cgctcatgta cattgccatt gaaggagtca tcggcgtcgg aaaaaccagc
      901 cttgcccgcc ttctacagcc ctcgtttaaa gccaatttat tacttgagat ttttgaagaa
      961 aatcctttct tgcaggaatt ctatcaagat cgtgctcgtt atgcttttca aacccagata
     1021 ttttttctct tgagccgcta ccgccagcaa cgtaataacg tgcctgtctt gttgcaaacc
     1081 ggcaagaacc tgcttaccga ctatactttt gaaaaagatg ccctctttgc agacatcaac
     1141 atcaccgggg atgaactgga tatgtattat tcggtacaca ctgctctcgc agaaaagatt
     1201 cccatccctg atctgaccat ttatctcaag gctagcaccg aggttttgat gcagcgtatt
     1261 gccacgcgcg accgctctta tgagcgttct atggaatcgg gctacattaa tgacctgaat
     1321 ggcgcttatg atgcattttt cagcaaatcc ggtgaaaata aaaaggtgct gaccatcgac
     1381 accaatgata ttgatttcgt tcacaacctc tccgatctcc agatgatcga caatcgcatt
     1441 cggcaaacct tgcacatgcc tcctttccaa ccctccctgc cgataatgaa ataaaaggat
     1501 gatgaaatga gagtaacaag gaacctgctc ctggaaaccg cccgcaatca cgtgaaatca
     1561 caaacgctga acaatcctga cattgcctgt gtttttctgg tggggtcctt attatccgat
     1621 aatcctttca taggcgaaaa caccgatatc gacctggtct ttatccacaa cactgaacct
     1681 gcggcacccc gcgaagtaat acctatggga gacgaattca atctggacat actacatttt
     1741 ccccgccgca tcttcaacca gcctaaaacc ctcagggaag acccctggct tgggtgtctg
     1801 ttttgtgaga aaccctttgt actttatgac tcttatcatt tttatgattt cttacgtgcc
     1861 ggtgtctatt caaatttctt ttcacccact tatgcttatg ctcgttctca gtatttttat
     1921 atccgcgcca ggaaacggtg gatggatttg catcaggcag aacaaggaaa aaatttctca
     1981 gtgagtttcg tgttcgctta cctgcaaact ctctacgatg ctgctaacac catcgcttgc
     2041 ctgacaggaa aaccaattcc agagaggcgc ttcctaacta cattagaagc aatcacaacc
     2101 tatcttggca gacccggtct cgccagtgga atgcgagatc tatttgctcc gactaactac
     2161 gacctcatcg attggcaaga tttgcaccaa caactaacga ttattttttc aatcctttcc
     2221 gataaatcat attgccctcc gcaatatgca cctgcccgtg ttaattatta tctcggtgct
     2281 gcctcttact atcaagttga acgattcgat gaatcgatct ggatattatt gtgggtttgg
     2341 accaacatca tgcaaatgct gccaaagcgc tctcccgaag tcaggggctg gaaggacttt
     2401 tgtgagcagt tgaacttttc gcgcgattcc attccactga agctacaaca attagatatc
     2461 tatctggatg cagtcgatga a
//
LOCUS       DAOSDS010000183         2452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_14984_length_2452_cov_3.218189, whole genome shotgun sequence.
ACCESSION   DAOSDS010000183 DAOSDS010000000
VERSION     DAOSDS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14984_length_2452_cov_3.218189"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PLL88_12025"
     CDS             complement(<1..374)
                     /locus_tag="PLL88_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOJ02330.1"
                     /translation="MKITTWNVNGIRAALKKDIAKWIGEFQPDVLCLQEVKATLEQVD
                     LAPILDLGYSVIWNAAQRPGYSGVATFYRNEPPQVITGIGIDKFDGEGRVIRTKYPDF
                     ELFNIYFPNGGQENERVPFKLD"
     gene            546..1865
                     /locus_tag="PLL88_12030"
     CDS             546..1865
                     /locus_tag="PLL88_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ02331.1"
                     /translation="MYVVQAGETLTLIAEKFNTTVDELISINGIVNADLISEGTELLI
                     PGLEGVTGRLITHAVNIGETLEDFSIANAIPLQTLLSLNKITSPNEIYVGTNIILPVS
                     DETNTLSVARTVKSQDTLLENAVRQQSNPWVLLQQNNLRTGNSLILSTPIFAQISSDQ
                     IVSTISPVIRDVEISPLPLVQGTTIVIKVEATEPIDLSGSLDGHILHFFPYQDQVNAY
                     YAIQGIHAMAEPGLVAFTLNGSLQGAPVFSYDQMLLQQAGTFIQDPPLYVQDETVDPA
                     ITGPEEELVMSYTSQISPEKYWGGSFRYPVDGSVEDDTIGFMSTFGNRRSYNGSEYTY
                     FHSGLDFGVYMNSLNIYAAESGTVVFAGSLTVRGNATFIDHGQGIFSGYFHQSQILVN
                     VGDYVQKGQLIGLIGSTGRVTGPHLHWEIWANGVQVNPVDWVLNTYP"
     gene            1919..>2452
                     /locus_tag="PLL88_12035"
     CDS             1919..>2452
                     /locus_tag="PLL88_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02332.1"
                     /translation="MVLSETSIKNTWDDFQLDSSDIEFIYSYLLEKEVPLPASDLLEA
                     VVVNRIKKEKELIIKKTKGKGKIFLPKDSYELKEELVFPAFGWKNGKVIDIRDGFNPE
                     LSSFKVIKVQFDDGEVKELACELADHPLNAPIDLIGDDPAFDKEKVLAERKEDFLERL
                     ETTLSSSKDLVQIAGAWF"
BASE COUNT          674 a          576 c          518 g          684 t
ORIGIN      
        1 aaatccagtt taaagggtac gcgttcattt tcttgcccgc cattgggaaa atagatgttg
       61 aacagttcaa aatccggata ttttgttcga atgacccttc cttccccatc gaacttatct
      121 ataccaatcc ctgtgatgac ctgagggggt tcgttcctat agaaagtggc tacgccgcta
      181 tatcctggtc gttgagccgc attccagatg accgaatatc caagatcgag aattggcgca
      241 agatctactt gctcaagtgt cgctttcact tcttgcaggc aaagcacgtc tggttgaaat
      301 tccccgatcc actttgcgat atcttttttc aaggctgcac ggattccatt tacgttccag
      361 gttgtaattt tcattgtcat cactgtggta aacttaatta gtttttgtgc tttaattgta
      421 gcagattgga agcacgtaca cataacatta tgcgcaagaa attttgtcta tcattactaa
      481 tttctatact ttttctacca ttcaacagca caatcatctt cgcgcaggac aatcttgaat
      541 atcccgtgta tgttgttcaa gcgggggaaa cgctcacact gatcgcagaa aagtttaata
      601 ccaccgttga tgaattgatt agcatcaacg gcattgtcaa cgccgacctc atctctgaag
      661 gaacggagct attgatccct ggtttggaag gggtaaccgg tcgcctgatc acacacgctg
      721 ttaacatcgg agaaacgctc gaagattttt ctattgcaaa tgccattcct ttacagacct
      781 tgctttcgtt gaacaaaatc acctcaccca atgagatcta tgttggtaca aacattatcc
      841 ttcctgtttc agacgaaaca aataccctgt cagtggctcg cacggtaaaa tcgcaagaca
      901 ccctgctcga gaatgcggtc cgtcaacaat ccaatccctg ggttttgctg cagcagaata
      961 acctccgcac aggcaattcg ctgatccttt ccactcccat tttcgctcaa atcagctccg
     1021 atcaaattgt tagcacgatc tctcctgtta ttcgagacgt cgaaatctct ccccttcctc
     1081 ttgtacaggg cacgaccatt gtgattaaag tcgaagcgac cgagccaatt gacctcagcg
     1141 gatcgctgga tggtcacatt ctgcattttt ttccatacca ggatcaagtc aatgcttatt
     1201 acgccatcca gggcattcat gccatggctg aaccgggact ggtagcgttc acgctaaacg
     1261 gaagtctaca aggtgcgccc gttttttcct atgaccaaat gctccttcaa caagccggca
     1321 cgtttatcca agaccctcct ttatatgtac aggatgagac tgttgaccca gccattactg
     1381 gtcctgaaga agaactggta atgtcataca cctcacagat atccccagag aaatactggg
     1441 gaggttcgtt tcgatatccc gtggacggct ctgtcgaaga tgacacgatt ggttttatgt
     1501 cgactttcgg taatcgccga tcgtataacg gaagtgagta cacgtatttt catagcggtc
     1561 ttgatttcgg agtctacatg aatagtttga acatttatgc tgccgaatca ggaacggtcg
     1621 tctttgccgg ttcattgacc gttcgtggaa acgccacctt cattgaccat ggtcaaggca
     1681 tattcagtgg atatttccat cagtctcaaa tattggttaa tgttggagac tacgtccaaa
     1741 aagggcaatt gatcgggcta atcggttcca ccggtcgcgt tacaggtcca cacttacact
     1801 gggagatctg ggcaaatggc gttcaggtca atcccgttga ttgggttttg aacacgtatc
     1861 cgtgaaaaat gctgctaagg tataattact ccgctcgaaa ggattttgat tatttcaaat
     1921 ggtattgtct gaaacgtcaa taaaaaacac ctgggacgat tttcaactag atagttctga
     1981 tatagagttc atttattcct acctcctgga aaaagaagta cctctgcctg catccgacct
     2041 cttagaggct gtggtcgtca atcgaataaa aaaagaaaaa gaactcatca ttaagaaaac
     2101 aaagggaaaa gggaagatct tccttcccaa agattcgtat gagctgaaag aagaacttgt
     2161 atttcctgcc tttggatgga agaacgggaa agtgatcgat atcagagacg gattcaaccc
     2221 cgaactttcc agctttaaag ttattaaagt ccaatttgat gatggagaag taaaagaatt
     2281 agcctgcgaa ctggcagatc acccgctcaa tgcccccata gatctaatcg gcgatgaccc
     2341 cgcttttgac aaagaaaagg ttcttgccga acgaaaagaa gactttcttg aacgccttga
     2401 aaccacgctt tcaagcagta aagatctggt gcaaattgcg ggcgcctggt tc
//
LOCUS       DAOSDS010000184         2255 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_16658_length_2255_cov_4.248182, whole genome shotgun sequence.
ACCESSION   DAOSDS010000184 DAOSDS010000000
VERSION     DAOSDS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16658_length_2255_cov_4.248182"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="PLL88_12040"
     CDS             <1..1074
                     /locus_tag="PLL88_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ02333.1"
                     /translation="GMKDLRDTETDLYKDPTDQDWFYHIRDLGGSHYLLTAVTKPTIN
                     LRTIVQDEFMKPIVQAVLIVIVLTFFISLIIGNWIALPLKRMSRSAEALSEGELIEIP
                     LEGPKEVRKLAHTFNLMSKKITASSQSQREFIANVSHEFKTPLTSIQGFAQAIKDGTV
                     KSKKDINKVAGVILIETNRLYRLMMDLLMLVRLDAGTANMCQEEIHMPDLLQNMVDKF
                     SLQAEQKGVALLTTHIPRVQVLGDGDRLAQVFSNLIDNALYYTPSGGKVEISAESDGR
                     EVSVSIQDLGIGIPDEEKEKIFERFYQINSSRKHGKKKGFGLGLSIAREIVQSHHGKI
                     WVEDNPDKGSIFFVKLPIIINKS"
     gene            1089..2078
                     /locus_tag="PLL88_12045"
     CDS             1089..2078
                     /locus_tag="PLL88_12045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ02334.1"
                     /translation="MKKTVSVIVPCYNEESTIQLLLDAILKQDFPLHEIEVIIADALS
                     QDETRGKIAAFAKAHPQLSTKIVDNPKRTIPAAVNAAAGAAQGTYLVRLDAHSMPYPN
                     YITTCIRDLEEGKGENVGGVWEIKPSADTAVARAIAAAAAHPLGVGDALYRYSDKAGE
                     VDTVPFGSFRRDMFFRIGGFDENLLTNEDYEFNVRIREGGGAIWFDPRIRCVYFARKN
                     LRELAKQYWRYGYWKLKMLKRYPATIRWRQALPPLFVLGNMLGIILSLFNQTIAYSYL
                     CILSVYLFMVLMAGIRAAVKKKDLALTWGLPAAISTMHVSWGMGFLVSILSKE"
BASE COUNT          600 a          503 c          583 g          569 t
ORIGIN      
        1 ggtatgaaag atttgcgcga cacagagacg gatctctata aggacccaac cgaccaggat
       61 tggttctacc atatccgcga tctgggaggc agtcattatc tgttgactgc tgttacaaag
      121 cccaccatta accttcgcac aatcgttcaa gatgagttta tgaaaccgat cgtgcaggcg
      181 gttttgattg tgatcgtgct gacgtttttc atcagtttga tcattggcaa ttggatcgct
      241 ttgccgttga aacgcatgtc cagatcggca gaagcccttt cagaaggtga gttgatcgaa
      301 attcctctgg aaggaccgaa agaagtgcgt aagctggctc ataccttcaa cctgatgagc
      361 aaaaagatca ctgccagttc gcagtcgcaa cgggaattca tcgccaatgt ttcccatgag
      421 tttaaaaccc ctttgacttc tatccagggg ttcgcccagg cgatcaagga tggtacggtg
      481 aaatccaaaa aagatattaa caaggtagct ggtgtgatct tgatcgaaac caatcgcctg
      541 taccgcttga tgatggattt gctcatgctg gttcgcctgg atgccggtac agccaatatg
      601 tgccaggaag aaatccacat gcctgactta ttgcagaaca tggtagataa gttctctctg
      661 caagccgaac aaaaaggcgt tgcgctctta accacccaca tcccgcgtgt acaggtgttg
      721 ggcgatggcg ataggttggc gcaggtgttt tctaacctga tcgacaatgc cttgtactat
      781 accccctctg gggggaaggt cgaaatctca gcagagagcg acgggaggga agtgtccgtt
      841 tcaatacagg atttgggaat cggaattccg gatgaggaaa aagagaagat cttcgaacga
      901 ttctatcaga tcaattcctc acgcaagcat ggaaaaaaga agggttttgg tttaggatta
      961 tccattgccc gtgaaattgt ccaatctcac catggaaaga tctgggtcga ggataatcct
     1021 gacaaaggaa gtatattctt tgtgaagctt ccaatcatta tcaataagag ttagcgcaaa
     1081 tggaaccaat gaaaaaaaca gtatccgtta tcgtcccctg ttacaacgaa gaaagtacca
     1141 ttcaactttt gctggatgcg atcctgaaac aagattttcc gctccatgag atcgaggtca
     1201 ttattgctga tgcgctctct caagatgaaa ctcgcggaaa gatcgcggca tttgcgaaag
     1261 ctcaccctca actgtccaca aagatcgtgg ataatcccaa acgcacgatc ccggcggcgg
     1321 tcaacgcggc ggcaggagcc gcgcagggca cctatctggt gcgcttagac gcgcattcca
     1381 tgccatatcc caactacata accacctgta tccgggatct ggaagagggc aaaggcgaga
     1441 atgttggcgg cgtgtgggag atcaaaccca gcgcggatac tgctgtagcg agagccattg
     1501 ctgcggctgc tgcccatccg ttgggggtgg gagatgcgct ataccgctat tctgacaaag
     1561 cgggcgaggt tgacacagtg ccatttggat cattccggcg agacatgttc tttcgcattg
     1621 gcggatttga cgagaacttg ctcaccaatg aagattatga gttcaatgtg cgcatcagag
     1681 agggcggtgg agcaatctgg tttgatcccc gcattcgctg cgtttatttc gcgcgaaaga
     1741 acttgagaga attggctaag caatactggc gttatggata ttggaaatta aagatgttaa
     1801 aacgctatcc tgccaccatt cgatggaggc aggcgcttcc gcctttattc gtgttgggaa
     1861 atatgctggg gatcatactt tcacttttca accaaaccat tgcgtatagc tatttgtgca
     1921 ttttgagtgt gtatctattc atggtgctga tggcaggaat cagagctgct gtgaaaaaga
     1981 aagacctggc gctgacctgg ggcttgcccg ctgccatcag caccatgcat gtttcatggg
     2041 gaatgggttt tctcgtaagt attttaagca aggaataatt gagtacaata tataaccgac
     2101 atggctaaca tgcagaaaac caaacataca cgctggttga tcaaatctca ggaacgacgc
     2161 tggatcctca ttttggggga cctgctggtt ggggtgattg cgctgtttat cgcgttgtat
     2221 atctgggcgc aaaaggacca atggatcagt ttttc
//
LOCUS       DAOSDS010000185         2216 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_17025_length_2216_cov_5.074040, whole genome shotgun sequence.
ACCESSION   DAOSDS010000185 DAOSDS010000000
VERSION     DAOSDS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17025_length_2216_cov_5.074040"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLL88_12050"
     CDS             <1..487
                     /locus_tag="PLL88_12050"
                     /inference="COORDINATES: protein motif:HMM:NF017509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02335.1"
                     /translation="EQVLHNFEHNLSHQNLDLDTFLKLNKMEKDDFIEKELTPVAKRQ
                     LEHSLVLEQLAEEEKIELKQEDLDKVYRQTMYEYQTRDDFNKIKRELSPKKLANSIFM
                     QAASRLMNHQVLDRIKQLANGEPVEKKEDAVQKTAKGTEKKAKKQDKPENKTEEGTRS
                     E"
     gene            516..1121
                     /locus_tag="PLL88_12055"
     CDS             516..1121
                     /locus_tag="PLL88_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOJ02336.1"
                     /translation="MKNQDFSNVIPMVIESSSRGERAYDIFSLLLRERIVFVGSAIND
                     QIANLIVAQLLYLNREDAEKEIHMYINSPGGQIYSGLAIYDTMKMIPNSISTVAVGVT
                     ASFGTVLLAAGTKGKRYALPHATIHMHQPLGGAEGQASDIEIQAKEILRLKARLNEIL
                     SSCTSQPIEVIEKDTERDLYLSAEQAVEYGLVDTVLIPPQP"
     gene            1201..1980
                     /locus_tag="PLL88_12060"
     CDS             1201..1980
                     /locus_tag="PLL88_12060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HOJ02337.1"
                     /translation="MNLINNSIKAVILDMDGVLWKSNDPLCDLNELFHQFEARHLKFL
                     FATNNALSTVAQYIQKFQSLGVNVSEEQIFTSAMATGYLLAKDFPQGGPVYVMGSPAL
                     QTTIQEYNFPFTEENPIAVVGGFDPDVSYDRLKKASLFIQSGLPFYFTNSDATYPTPE
                     GFAPGAGTFLAALETASGVKAKVAGKPQPYLFEACLERLGTKPDETLTVGDRLETDIL
                     GGYNAGCKTALVLTGIATRQDLQTWAPQPDMVIDNIMQLFD"
     gene            1986..>2216
                     /locus_tag="PLL88_12065"
     CDS             1986..>2216
                     /locus_tag="PLL88_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C2)-methyltransferase"
                     /protein_id="HOJ02338.1"
                     /translation="MKCILDLDTEELQTYFQSIHQPAFRCKQVWQGLYHSHFDAWDQF
                     TVLPQTLRQKLAQEFIISPLQVIGSLESPDGST"
BASE COUNT          643 a          527 c          503 g          543 t
ORIGIN      
        1 cgaacaggtg ttacacaatt tcgaacacaa tctctctcac cagaacttgg atctggacac
       61 cttcttgaaa ttgaacaaga tggaaaagga cgatttcatt gaaaaagagc tgacgccggt
      121 tgcaaaacgt caattggaac actctttggt gctcgaacag cttgcggaag aagaaaagat
      181 cgaactgaaa caagaagatc tggacaaggt ttatcgtcaa accatgtacg aatatcaaac
      241 ccgcgacgac ttcaacaaga tcaaaagaga gttatcgccc aagaagcttg ccaattctat
      301 ttttatgcaa gctgcttctc gtttgatgaa ccaccaggtg ctggatcgca tcaaacaact
      361 ggcaaatggt gaaccggtgg agaagaagga agatgctgtt cagaaaacag caaaaggcac
      421 ggagaaaaaa gccaagaagc aggacaaacc cgagaataaa accgaggaag gaacacgatc
      481 tgagtgattt cattcctgga gaaagaggta aataaatgaa aaatcaagac ttttccaatg
      541 tcatccccat ggtgatcgaa tcatccagcc gtggggaacg agcctatgat atcttttcgc
      601 tactgctacg cgaacgtatc gtttttgtcg gatcagctat caacgatcaa attgcaaatc
      661 tcattgtggc tcaattgctc tatttgaacc gggaagatgc agaaaaagag atccacatgt
      721 acattaactc gccaggtggg cagatctact cggggttggc aatctacgac acaatgaaaa
      781 tgatccctaa ctctatcagc acggtggctg ttggagtaac cgcatctttt ggcacggttc
      841 tcctggctgc aggcacgaaa gggaaacggt atgctctgcc gcacgccacc attcacatgc
      901 atcagccact ggggggggca gaagggcagg cgtctgatat cgagatccaa gccaaagaga
      961 tcttacgcct taaagctcgt ttgaatgaga ttctttcaag ctgcaccagc caaccgatcg
     1021 aagtgatcga aaaagatact gagcgcgatt tatatctgag cgccgaacaa gcggtcgaat
     1081 acgggctggt cgatacagtt ctcattccac cacaacctta accaccgaat gatgtacaat
     1141 aatgccggat gccagtttgg catgcggcat ttttatttta ttggagaatg ggagaaaagg
     1201 atgaatctta ttaataatag tattaaagcc gtgatcctgg atatggacgg cgtactgtgg
     1261 aagagcaatg accccttatg tgatcttaac gaactctttc atcaatttga ggcacgtcat
     1321 ctaaaatttt tatttgccac caacaatgca ctgagcactg tagcgcaata catccagaaa
     1381 tttcaatcat taggcgtaaa cgtttcagaa gagcaaattt ttacttctgc gatggcaacc
     1441 ggttacctgc ttgccaaaga tttcccgcag ggaggacccg tttacgtcat gggttctccg
     1501 gctctgcaaa ccaccatcca ggaatacaat ttcccattca cagaggaaaa ccctattgcc
     1561 gtagtggggg ggttcgatcc tgatgtttct tatgatcggc tgaaaaaagc ctctctgttc
     1621 attcaatcag ggcttccttt ctattttacc aattccgacg ctacctaccc cacccctgag
     1681 gggttcgctc caggcgcagg gacatttttg gcagccctcg agactgcttc aggagtaaaa
     1741 gcaaaagtgg ctggaaaacc gcaaccctat cttttcgaag cctgtctgga gcgtttgggt
     1801 acaaaacctg acgaaacact cacggtcggc gatcgccttg agacggatat tttgggtggt
     1861 tacaacgccg gctgtaaaac agctctggtt ctcacaggca ttgccacccg ccaggacctg
     1921 caaacctggg ctccccaacc agatatggtg atcgataata tcatgcagtt atttgattga
     1981 tctttatgaa atgtattctt gatctggata cagaagagct ccagacttat ttccaatcca
     2041 tccaccaacc tgcattccgt tgcaagcagg tctggcaagg tttgtatcat tcccattttg
     2101 atgcctggga tcaattcacg gtccttcccc aaaccttgcg gcagaaactt gcccaagaat
     2161 tcatcatttc ccctcttcag gtgataggtt ccctcgaatc tccagatggg tcgacg
//
LOCUS       DAOSDS010000186         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_18008_length_2122_cov_4.219642, whole genome shotgun sequence.
ACCESSION   DAOSDS010000186 DAOSDS010000000
VERSION     DAOSDS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18008_length_2122_cov_4.219642"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLL88_12070"
     CDS             complement(<1..656)
                     /locus_tag="PLL88_12070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02339.1"
                     /translation="MKNNRTSFSTLLVVLVIGSMFLVGCGNSTSIADPTPVPSLTAAL
                     AQAPTRVSSPTAAPVPLPALTLESGAFYFSLDGVQNFLFSRNLAGVTPWQYSQLLDLI
                     SFGSSRVIRLSLDSMGTGITPGGEVDEVWAGKWEQVLDDAAAQGIYVIPVFSTWYDWN
                     NGTPDLGYSQWGSNAFNVANGGAAATPADLFLAGSPTQTLWLAWVEKLVQRWQNRENI
                     "
     gene            complement(892..1077)
                     /locus_tag="PLL88_12075"
                     /pseudo
     CDS             complement(892..1077)
                     /locus_tag="PLL88_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
     gene            1195..1914
                     /locus_tag="PLL88_12080"
     CDS             1195..1914
                     /locus_tag="PLL88_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOJ02340.1"
                     /translation="MQQKITENRSYRFFDLVMALFVAVLITSNIASSAKIVDWGFAVW
                     GVRMAFDAGTLLFPISYIFGDVLVEVYGFKQSRRVIWVGFAALALCALTLGVVRALPG
                     EAVWQEQTGQVAYDAVLGGMSSGGIVIASLVAYFLGEYSNSVVMAVMKVKTEGKFLWL
                     RTITSTLVGEGVDSLFFTLIATLFGVFPWEIFSSLVLTNYIFKVAVEVLFTPLTYRMV
                     NRLKKAENEDYYDRGTRFSPL"
     gene            complement(1909..>2122)
                     /locus_tag="PLL88_12085"
     CDS             complement(1909..>2122)
                     /locus_tag="PLL88_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOJ02341.1"
                     /translation="TLVFYESPYRLLGFLEDAMAVYGDRKAALANDLTKKFEAVLRGT
                     LSEIKQQLSEEKLLGEYTIVIAGKDS"
BASE COUNT          500 a          547 c          551 g          524 t
ORIGIN      
        1 ataatgttct cgcggttctg ccagcgttga accaactttt ccacccacgc cagccacaga
       61 gtttgggtgg gcgaacccgc caggaacagg tcagccgggg tggcagcagc ccccccatta
      121 gcaacgttga aggcattcga cccccattgg gaatagccga gatccggggt gccattattc
      181 caatcatacc aggtggaaaa tacgggaatc acgtagatgc cctgggcagc cgcatcatcg
      241 agcacctgct cccacttccc cgcccaaacc tcatccacct ccccaccagg ggtgataccg
      301 gttcccatac tgtccaatga gaggcggatc acccggctgc tgccgaagct gatcagatcc
      361 agaagctgtg agtattgcca tggcgtgacc ccagccaggt tcctggagaa aagaaagttt
      421 tgtacgccat ccaggctgaa atagaacgcg cccgactcaa gtgtgagcgc gggcagcgga
      481 acgggggcgg cggttggaga ggagacgcgg gtgggcgcct gagcgagggc agcggtcagg
      541 gaaggcaccg gcgtggggtc agcaatcgag gtggagttgc cacagcccac aagaaacata
      601 ctgccaatga ccagcaccac cagaagggta gagaaggagg ttctattgtt tttcatttac
      661 cgaaattccg cgtctgatat tttttgcaac catttttccc caagaaagat gataattatg
      721 gctgaactta ctatatttta acattgtttc ctccatttgc atcatccttt gcgatccatt
      781 ttataaaatc ggatgttggg aatgattttt ctcagcctgc cttaaatacc ataacagatt
      841 gggacataaa aagcgcgttt tttcaggaaa ggcttttgca tagatttctt gctaaggata
      901 atttgttgag agtttcaaca ccatacttct tgccattctg atgatctttc ctgccagaaa
      961 tatgtacttc agcctgaacg ttttgatttg ctgccggcgt tccgttccat ttgcaaagtc
     1021 catcttgaac aacaaacata cattgtaagc cagcatcatc aactgaaaaa tggcttcaat
     1081 ttgatgaaat attggctaaa aacacctgct ttatcataga aagcagggct tttaatcaaa
     1141 tgtcatgttt tgaccaattc ggcgacgtgg aatccgggtt atactaaacg cttgatgcaa
     1201 caaaaaataa ccgaaaatcg cagttatcgc tttttcgatc tggtcatggc gctctttgtg
     1261 gccgtgctca tcaccagcaa tatcgcctct tccgccaaga tcgtggattg gggctttgca
     1321 gtgtggggcg tgcgcatggc gtttgacgcc ggtacgctgc tctttccgat cagctacatc
     1381 ttcggggatg tgctggtgga agtatacggt ttcaagcaat cccgcagggt gatctgggtt
     1441 ggttttgctg cgctggcgct gtgcgccctc actctggggg tggtgcgtgc cttgcccggc
     1501 gaagcggtct ggcaggagca gaccggtcaa gttgcctatg atgctgtact ggggggcatg
     1561 agcagcggcg gcatcgtcat cgccagcctg gtggcgtatt ttttggggga atacagcaac
     1621 tcggtagtca tggcggtcat gaaagtgaaa accgaaggaa aattcttgtg gctgcgcacc
     1681 atcaccagca ccctggtggg ggaaggcgta gattcgctgt tcttcacgct catcgccacg
     1741 ctgttcggcg ttttcccctg ggaaatcttt tccagcctgg tgctgaccaa ttatattttc
     1801 aaagtcgcgg tagaagtgct cttcaccccg ctcacctatc ggatggtgaa ccgtttgaaa
     1861 aaagcggaga acgaagatta ttacgaccgc ggcacgcgct tcagcccgct atgaatcttt
     1921 gcccgcaatg acaatggtat attcacccag cagcttctct tcggagagct gctgttttat
     1981 ctccgacaac gttccgcgca gcaccgcctc gaacttcttg gtcaaatcat ttgccagggc
     2041 agccttgcgg tcgccataca ccgccatggc atcctccaga aaacccagca gtcgataagg
     2101 gctttcatag aacaccaggg tg
//
LOCUS       DAOSDS010000187         2118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_18045_length_2118_cov_3.664566, whole genome shotgun sequence.
ACCESSION   DAOSDS010000187 DAOSDS010000000
VERSION     DAOSDS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18045_length_2118_cov_3.664566"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PLL88_12090"
     CDS             <1..1414
                     /locus_tag="PLL88_12090"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOJ02342.1"
                     /translation="FGYIIAWREDRLYRSMRAMLTVIDTIQDNHIQVLLARESFDPMM
                     APLKAWVASMELKGMRERMSMGVKARLRNGKANTGQDRYGYRRNGEIIEIVEEEARWV
                     RKVFEWYNQGVKLMEIRRRLIEARAPQKGSTRLRKIEWAVTSIQSILQAADAYYRGVK
                     KQSRDGEVFEIPAPPIIDSKTYERYRIVRERNVKHPMFNYRHDYLVSGLLKCACGRKW
                     AGRTQSSATRKNRKGEVVPRKTMSSTYCCTQHHKELIHEDCPRSIGHLKADDYVWGKV
                     KGAIDDPEILIARARDYVDDLRKKAKSANEDNDRIQRELDNLVLERKWVITQARKGSI
                     SEQDMDEQLADLSAQEITLKQEMEMVTDQSLLSALNQWEEHAREYFADLKVGLLELNT
                     VPQTDEERLEQFELKRKIVNTLVEKVYIHKDRSLEVTIRLNLLQMIRQRPNADQVPQV
                     GTYTRRSAYPFRRHPGGCGG"
     gene            complement(1326..1733)
                     /locus_tag="PLL88_12095"
     CDS             complement(1326..1733)
                     /locus_tag="PLL88_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HOJ02343.1"
                     /translation="MEVPRNWRLQKQRLALVGEICPHCQAKIFPARDVCPQCGGEAKT
                     AFKFSGKGEVYSFTTMYSVPSGFEQNAPYTVALVKLEEGPIITAQLTDVGEREVKIGT
                     PVEMVTRRIRQDGDERGMLIYGYKFRPVGLGPH"
     gene            complement(1819..2106)
                     /locus_tag="PLL88_12100"
     CDS             complement(1819..2106)
                     /locus_tag="PLL88_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02344.1"
                     /translation="MLSFEAAGFAERGKGWQVATPVALALDGRLPIASLGGIKARGFA
                     PGAAGVYQVVDAVMQLRGEAGQNQITDAHTAMTQALSGAADSAITTILRTL"
BASE COUNT          569 a          489 c          554 g          506 t
ORIGIN      
        1 ctttggttac attattgcgt ggagagaaga tcgtttatat cgcagtatgc gggctatgct
       61 gacggttata gatactatcc aggataatca tattcaagtc cttcttgccc gagaatcatt
      121 cgatcccatg atggccccac tcaaagcatg ggtggccagc atggaattaa aagggatgcg
      181 tgaacgcatg agcatgggag ttaaggctcg tttacgtaat gggaaagcaa acaccgggca
      241 ggatcgctat ggttatcgta ggaatggtga aattattgaa atcgttgagg aagaagccag
      301 gtgggtacga aaagtttttg aatggtataa ccagggcgtc aaactgatgg agatccgaag
      361 gagattgatt gaagcgcgtg ctcctcaaaa aggctcaact cgtcttcgga aaattgaatg
      421 ggcagtcacc tcaattcaat ccattttaca agctgccgat gcatattatc gcggggttaa
      481 gaaacaaagt cgagatggag aagtattcga gattcctgca ccaccaatta ttgattccaa
      541 aacatatgaa cggtatcgaa ttgttcgaga acgaaacgtt aagcatccta tgttcaacta
      601 tcgccatgat tacctggtca gtggattatt gaaatgcgcc tgtgggcgta aatgggccgg
      661 cagaacccaa tcttctgcaa cccgaaagaa ccgtaagggt gaggttgtgc cgcgtaagac
      721 catgtccagc acttattgct gcacccaaca ccacaaggaa ctgattcacg aggactgtcc
      781 tcgcagcatt ggtcatctca aagcagatga ctatgtttgg gggaaagtaa aaggtgcgat
      841 tgatgaccct gagattctca tcgcaagggc aagagattat gtagatgatc tacgtaagaa
      901 ggcaaaatcc gcaaatgaag acaacgatcg gatacaacga gagttggata atctggtttt
      961 agaacggaaa tgggtgatta cccaggcgcg aaaaggaagt atttcagagc aagacatgga
     1021 tgagcagtta gcagatcttt cagcccaaga aattacattg aagcaggaaa tggagatggt
     1081 aactgatcaa tctttactgt ctgccctcaa ccagtgggaa gaacatgcgc gggaatattt
     1141 tgctgatctt aaggtcggtt tattggaatt aaataccgtt cctcaaacag atgaggaacg
     1201 gcttgaacaa tttgagttga aacgaaagat tgtaaatact ttggttgaga aggtctacat
     1261 ccacaaagat cggtctttag aggttacgat ccgtttaaat ctcctccaga tgattcggca
     1321 acgacctaat gcggaccaag tcccacaggt cggaacttat acccgtagat cagcataccc
     1381 ctttcgtcgc catcctggcg gatgcggcgg gtgaccatct ccaccggggt accgatcttc
     1441 acttcgcgct cgcccacatc ggtgagctgc gcggtgatga tgggtccttc ttccagcttg
     1501 accagtgcca cggtgtaggg cgcgttctgt tcaaaaccgg atggcacact atacatggtg
     1561 gtgaaggaat acacttcgcc tttgccgctg aacttgaaag ccgtcttggc ttcgccaccg
     1621 cattgcgggc agacatcgcg ggcggggaag atcttcgcct ggcagtgcgg gcatatttcg
     1681 cccaccaagg caaggcgttg tttttgtaat cgccaattgc gtggtacttc cattcgtaat
     1741 cactcctttt atgagagtga tgctagttta aagagctttc tctctcaaaa gctatcaatt
     1801 tttgaacctg atagaaattt acagtgtccg caaaatggtg gtaatcgcgc tgtcagccgc
     1861 gccgctgagc gcctgggtca tggcggtgtg cgcatccgtg atctggttct ggccggcttc
     1921 tccgcgcaac tgcatgacgg catccaccac ctggtagaca cctgccgctc cgggcgcaaa
     1981 accgcgcgcc ttgatgccgc ccaggctggc gataggcaat ctgccatcca atgccagggc
     2041 aaccggggta gcaacctgcc aacccttgcc gcgttcggcg aacccggcag cctcaaaaga
     2101 aagcatgccg tagatgga
//
LOCUS       DAOSDS010000188         1916 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_20472_length_1916_cov_3.193444, whole genome shotgun sequence.
ACCESSION   DAOSDS010000188 DAOSDS010000000
VERSION     DAOSDS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20472_length_1916_cov_3.193444"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(215..391)
                     /locus_tag="PLL88_12105"
     CDS             complement(215..391)
                     /locus_tag="PLL88_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02345.1"
                     /translation="MYSFIPAILFFGIAVWLYRRELKKDADSRNKALIMLASYAMILA
                     LLLLGLSIINWGQL"
     gene            complement(446..1294)
                     /gene="mutM"
                     /locus_tag="PLL88_12110"
     CDS             complement(446..1294)
                     /gene="mutM"
                     /locus_tag="PLL88_12110"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HOJ02346.1"
                     /translation="MPELPEVETIARALRQENRRGFSITGSEIEDVSILWERSIAIPS
                     AVEFKQRVCGQHIREVGRRGKYIVVTLTQDTLLIHLRMSGDLRIEQGGADVLKNGLHK
                     HDRVAFTLSGGNYLIFEDARKFGRLWLLADPQEELGTLGPEPLGEELDALRFYAMLQG
                     KKRQLKPLLMDQHFLAGMGNIYTDEALFKARLHPLLNAVDLNAEQTASLLRAIRETLQ
                     EGIARNGSSIDWVYRGGDFQNHFAVYGRTGEACPVCGATIERLVVGQRGTHICPQCQA
                     LPSRDE"
     gene            complement(1316..>1916)
                     /gene="prs"
                     /locus_tag="PLL88_12115"
     CDS             complement(1316..>1916)
                     /gene="prs"
                     /locus_tag="PLL88_12115"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HOJ02347.1"
                     /translation="GADRYMTMDLHADQIQGFFSIPGDVLYASHVLVDKLLELKKQLN
                     NPVLLSPDLGFAKKARNYAEQLNIPLAFIEKRRILHPDNPEALTLIGDVKGKDVILLD
                     EEISTGKTIINAVNVAVKSGAKDVVVVFIHPILGDDGAQQLAALPVKQFITTDTIPIP
                     DEKKKFFGKRLSVISVSKLLADVIERVNAGRSVGEMFNE"
BASE COUNT          379 a          553 c          469 g          515 t
ORIGIN      
        1 tgatgttcat cctgcccctc attgtcatct ttgtgctggt ttactttggc acttcttcca
       61 agcagctcac taccttcatc cagaaacgtg gatcgggtat caagctcgct cttgccatct
      121 tgttcctggt gctggcgatc tggttgggtt ggtctgtgtt gatgaaatga tacctcttga
      181 aacaaacaga gaccggtctt cgtgccggtc ttttttataa ttgtccccag ttgatgatgg
      241 aaagccccag cagcagcagt gccaggatca tggcatagct tgccagcatg atcagggctt
      301 tattacggct gtctgcgtcc ttttttagtt cgcgccggta tagccagacg gcaatcccga
      361 aaaatagaat ggcagggata aatgaataca tgtttttttc ctctgatttc cattttactt
      421 acgattgggg ataattccag ttctttcatt cgtctctgga tggcaacgct tggcattgtg
      481 ggcaaatatg cgttccccgt tgacccacca ccagccgctc gatggtcgct ccgcataccg
      541 ggcaagcttc tcctgttctg ccatatacag cgaaatggtt ttgaaaatca ccgccccggt
      601 acacccaatc gatgcttgaa ccgttgcgcg caatgccctc ctgtagcgtc tcccggatgg
      661 cgcgcagcag gctcgccgtc tgctctgcat tcagatctac cgcgttcagc agcgggtgca
      721 gtcttgcttt gaacagcgcc tcatcggtgt agatattgcc cattcccgcc agaaagtgct
      781 ggtccatcag cagtggcttg agctggcgct tcttgccctg cagcatggca taaaagcgca
      841 gcgcgtccag ttcctccccc aatggttccg gacccagcgt acccaattcc tcttgcgggt
      901 cagccagcag ccacaatcgt ccgaatttgc gcgcgtcttc gaagatcagg tagttgccgc
      961 ccgacagtgt gaacgccacc cggtcatgtt tgtgcagccc atttttaagc acatccgccc
     1021 caccttgttc gatgcgcagg tcaccgctca tgcgcaggtg gatcaatagg gtgtcctgtg
     1081 tcaaagtgac gacgatgtat tttcctcgcc tgccgacctc tcggatgtgc tgtccgcaca
     1141 cgcgttgctt gaattccacg gcgctcggga tggcgatcga gcgctcccat aaaatagaaa
     1201 catcctcgat ctcgcttccc gtgatggaaa accctcttct gttctcctgt cgtagcgcac
     1261 gcgcgatggt ctcaacttcc ggtaattctg gcatttttaa atcatgtttg agtcgttatt
     1321 cgttgaacat ctcgcccacc gagcgtcccg cgttcacccg ttcgatcaca tccgccagta
     1381 gtttggatac cgagatgacc gacaaccgtt tcccgaagaa ttttttcttt tcatccggga
     1441 tggggatggt atcggtggtg atgaactgct tcactggtag cgccgccagt tgctgcgccc
     1501 cgtcatctcc caatatcgga tggatgaaca ccactaccac atccttcgca cctgacttga
     1561 ctgccacatt gaccgcgttg atgatggtct tgccggtcga gatctcttcg tccagcaaga
     1621 tcacatcctt gcccttcaca tcaccgatca gtgtcaatgc ctccgggtta tccgggtgca
     1681 ggatgcgccg cttttcgatg aacgctagtg gaatattgag ctgttccgcg taattgcgcg
     1741 ctttcttggc aaatcccagg tcaggcgata acagcaccgg gttgttcaat tgtttcttga
     1801 gttccagcag tttatccacc agcacatgcg atgcatacag cacgtccccc gggattgaga
     1861 aaaatccctg gatctgatcg gcgtgcaggt ccatcgtcat gtagcggtca gcgccc
//
LOCUS       DAOSDS010000189         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_20588_length_1908_cov_4.687534, whole genome shotgun sequence.
ACCESSION   DAOSDS010000189 DAOSDS010000000
VERSION     DAOSDS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20588_length_1908_cov_4.687534"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLL88_12120"
     CDS             complement(<1..411)
                     /locus_tag="PLL88_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOJ02348.1"
                     /translation="MISFSNVTSNRRTKIIATLGPSSDTAEIIEELVRSGIDVARLNF
                     SHGTHESHQNLIQLIRQAAEKLDETVAILQDLQGPKLRVGDLPSGSIQLKAGQIIRFV
                     CADRCQDAQARSDMPTIPLDIPDVFSSMSIGNRVL"
     gene            complement(419..1615)
                     /gene="rocD"
                     /locus_tag="PLL88_12125"
     CDS             complement(419..1615)
                     /gene="rocD"
                     /locus_tag="PLL88_12125"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOJ02349.1"
                     /translation="MKTADYVNLSDLYGAHNYQPLDVVLSRGEGVWVYDVEGNKYLDC
                     LSAYSALNQGHVHPAILKALVEQAQKITLTSRAFRNDQLPLFYKEIVDLTGYEMVLPM
                     NSGAEAVETALKVARKWAYVVKKVPRHKAEVVVADGNFHGRTISIISFSSEAFYKDDF
                     GPHTPGFISVPYGDIHALKKAITPNTAAILLEPIQGEGGVIIPPSGYLKAVREVCDQN
                     TILFIADEIQTGLGRTGKLFACDYENVRPDLMVLGKALGGGFYPVSAVLSSRAILGLF
                     QPGEHGSTFGGNPLAAAVARASLKVIVEENLIENAASLGEYFRDQLEEIPSKHVKEVR
                     GKGLLIGVELKPEAGGARQYCERLKDAGILAKETHETTIRFAPPLIIDQPAIDWALER
                     IRTVLL"
     gene            complement(1622..>1908)
                     /locus_tag="PLL88_12130"
     CDS             complement(1622..>1908)
                     /locus_tag="PLL88_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOJ02350.1"
                     /translation="EAVKDADVIYTDTWTSMGQEEETQKREKVFPPYQLNQALLAEAH
                     PDAIVLHCLPAHRGQEITDEVADGPHSRLFPQAHNRLHAQKAIVLKLMQG"
BASE COUNT          454 a          460 c          472 g          522 t
ORIGIN      
        1 caaaacgcgg ttgccgatag acatggaaga gaacacatca ggaatgtcca atgggatggt
       61 tggcatatcg cttcgtgctt gggcatcttg gcatctatca gcgcatacaa aacgaatgat
      121 ttgtccggct ttcagttgaa tcgatcctga tggcaggtct cccacccgta gcttgggacc
      181 ttgcagatcc tggaggatgg caacagtctc atccagtttt tcggctgcct gacggatgag
      241 ttggatcagg ttttgatgac tttcgtgcgt gccatgtgag aagttcaggc gggcgacgtc
      301 tattccgctt cgtacaagtt cttcaataat ctcagcggtg tcgctgcttg gacctaaggt
      361 ggcaatgatt tttgtgcgac gattggatgt gacattcgaa aaagatatca tcaggtgctc
      421 acaacaaaac ggttcggatt ctctccagcg cccaatcgat ggcaggctgg tcaatgatca
      481 aaggtggggc aaagcggatg gtcgtttcat gcgtttcctt tgctaaaatg ccagcgtctt
      541 ttaacctttc acaatattgt ctcgctccac cggcttcagg ttttaattct accccgatca
      601 agagaccttt gccccgcact tcttttacat gtttgctggg tatctcttcc aactggtcac
      661 gaaaatattc tcccagtgaa gctgcatttt caatgaggtt ttcttccacg atgaccttca
      721 aggaggcacg cgcaaccgct gccgctaaag gatttcctcc gaaggtagaa ccgtgttcac
      781 ctggttgaaa caacccaagg attgccctgc ttgataaaac tgccgacacc gggtaaaaac
      841 caccccccaa tgccttgcca agcaccatca gatcagggcg aacattttca tagtcgcatg
      901 cgaacagttt accggttcga ccaagaccgg tttgaatttc gtcagcaatg aacaaaatag
      961 tattttgatc gcagacctct cgaactgctt tcaggtagcc gctgggtgga atgatcaccc
     1021 cgccttctcc ttgaatgggt tcaaggagga ttgcagccgt attcggcgtg atggctttct
     1081 taagagcatg gatgtctcca tagggaactg agatgaagcc gggtgtatgg ggaccaaaat
     1141 catctttgta gaatgcttcg gatgaaaagg aaataatgga aatggttcgc ccatggaagt
     1201 tgccatcggc aactacgacc tctgctttat gccggggaac ctttttgaca acgtacgccc
     1261 atttgcgagc gacctttaat gcggtttcaa ccgcttcggc accgctgttc attggaagca
     1321 ccatttcata cccggttaga tcaacgatct ctttgtaaaa gagcgggagt tggtcattgc
     1381 gaaaagcacg cgaagtgagc gtgatcttct gtgcttgctc tacaagtgct tttaagatgg
     1441 ctgggtgtac atgaccctga ttgagagctg agtacgcact caagcaatcg aggtatttat
     1501 tcccttccac atcatacacc caaacccctt ctcctctgga gagcaccaca tccaatggtt
     1561 gatagttatg agctccatac aaatcggaga gattaacata atcggctgtt ttcatgtgag
     1621 tttatccttg cattaatttt agaacgatgg ctttttgagc gtgcaaacgg ttatgcgctt
     1681 gtgggaaaag tcgtgaatgg ggtccgtctg ccacttcatc tgtgatctcc tgccctctat
     1741 gggctggcag acaatgcaga acaatggcgt caggatgcgc ttctgccaga agcgcttgat
     1801 tgagctgata aggtgggaag actttctcgc gtttttgggt ttcctcctcc tgacccatgc
     1861 tggtccaggt atcggtatag atcacgtcgg catctttcac ggcttcgt
//
LOCUS       DAOSDS010000190         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_22010_length_1811_cov_3.965831, whole genome shotgun sequence.
ACCESSION   DAOSDS010000190 DAOSDS010000000
VERSION     DAOSDS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22010_length_1811_cov_3.965831"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /gene="radA"
                     /locus_tag="PLL88_12135"
     CDS             <1..914
                     /gene="radA"
                     /locus_tag="PLL88_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOJ02351.1"
                     /translation="DLDAILVHAQQICPQLLIVDSIQTMLMNAFTSSAGSVTQVRECA
                     SQLREFAKASGIAVFLIGHVTKEGMIAGPRVLEHIVDTVLQLEGDRFQSYRVLRSMKN
                     RFGPTSEVGVFEMREAGMVEVSNPSEAFLAERMENTPGSAVAVAMEGTRPLLIEIQGL
                     TSTTNFGNPRRTSNGVDFNRLLLVIAVLTRRNHLKLYDQDVFANVVGGFSVDEPAADL
                     AVATAIASSLLDKPVQPDMVLIGEVGLSGELRWAGQMASRLKEAAKMGFKKAIIPKRI
                     RKIEKLPDGIIVIEARSLSDAVKSALG"
     gene            935..1453
                     /locus_tag="PLL88_12140"
     CDS             935..1453
                     /locus_tag="PLL88_12140"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HOJ02352.1"
                     /translation="MENILEMNPVWKVRSNHALEHATLHVLSTKVTRARVSGISDAGG
                     FWLLGDIPTNLVMESAREALQRMQAGETGLAMHENCGSNLVPSIVVSGGLAWLAMTGT
                     GDSLRKKARRIPLAVLMALLGFEVAKPVGPVLQSKMTSTDHFDGMLVREVLCYKTYVF
                     VIHRVTTEFQAG"
     gene            1458..>1811
                     /locus_tag="PLL88_12145"
     CDS             1458..>1811
                     /locus_tag="PLL88_12145"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ02353.1"
                     /translation="MPENSTPEKEDVRVYVFIGDDLDAMANAESELVEELGNAEMAAF
                     NIAHLNGNTNNLDELVKNISLLPFGSLRRLVIDVDPLARISADKEVERWKHIVENLPP
                     SAELVLEIITSWVKKQ"
BASE COUNT          461 a          411 c          521 g          418 t
ORIGIN      
        1 cggatctgga tgcgattctg gtgcatgccc agcaaatctg cccccaactg ttgatcgtgg
       61 attccatcca gactatgtta atgaatgcct tcacctcgag tgccgggtcg gtgacccagg
      121 tgcgtgaatg tgcttcacaa ttgcgggaat ttgccaaggc gagcgggatc gcagtattct
      181 tgatcggaca cgtcaccaaa gaaggtatga tcgccggacc gcgtgtgctg gagcatatcg
      241 tggatacggt gctgcaactg gaaggtgacc gttttcaatc ctaccgtgtg ttgcgatcga
      301 tgaaaaatcg cttcggacca acttcggaag taggggtttt tgagatgcgt gaagcaggta
      361 tggtggaagt cagcaatcca tccgaagcct tcctggcgga gcgcatggaa aatacccctg
      421 gctcggcggt agcggtagcg atggagggga cacgcccgct gttgatcgaa atccaggggc
      481 tgaccagcac cactaatttc ggcaatccgc ggcgcacttc caacggggtc gatttcaacc
      541 gcttgctgct ggtaattgcc gtactgactc gccgcaacca tctgaaatta tacgaccagg
      601 acgtgtttgc gaacgtagta ggtggtttca gcgtagatga accggcggct gacctggcgg
      661 ttgcaacagc catcgcttct tccttgctcg ataaaccggt gcaaccagat atggtgctta
      721 ttggagaggt gggactttcg ggagaattgc gctgggcggg gcagatggca tcccgtctca
      781 aagaggcagc caagatgggt ttcaaaaaag ccattatccc caaacgcatc cgcaagatcg
      841 aaaaattacc ggatggcatt atcgtgatcg aggcgcgttc actcagcgat gcggtaaaat
      901 cagcacttgg ttaaatcaaa taaaagaggt cataatggaa aacatcctgg aaatgaaccc
      961 ggtctggaaa gtgcgctcga atcacgctct tgaacatgcc accctgcatg tcttatcaac
     1021 caaggtaacg cgcgcacggg taagcggaat atcggatgca ggagggttct ggttgttggg
     1081 tgacattcct accaatttgg tcatggaaag cgctcgtgaa gcattgcagc gaatgcaggc
     1141 aggcgaaaca gggctggcaa tgcatgaaaa ctgcggttct aacctggtgc ccagcattgt
     1201 ggtttctggc ggattagcct ggctggcgat gacaggcacc ggggatagcc tgcgtaagaa
     1261 agctcgcaga attccactgg cggtgctgat ggcattgctg ggattcgaag ttgctaaacc
     1321 agtgggaccg gtattgcagt ccaaaatgac ctcaacggat cattttgacg gtatgctggt
     1381 gagggaagtg ttgtgctata aaacctacgt ctttgtcatt catcgggtta ccactgaatt
     1441 ccaagcggga taaggatatg ccagaaaact cgacacctga aaaagaagat gtgcgcgtgt
     1501 atgtttttat tggggatgac ttggatgcga tggcaaacgc agaatccgag ttggttgaag
     1561 aattaggaaa tgcagaaatg gctgctttca atattgccca tctgaacggt aataccaata
     1621 acctggatga actggtgaag aacatcagct tacttccttt tggaagcttg cggcggctgg
     1681 tgatcgatgt cgacccactg gcacgcatca gtgcggataa agaggtagaa cgttggaaac
     1741 acatcgttga gaatctacct ccctcagctg aactggtgtt ggaaatcatc accagttggg
     1801 tgaagaagca a
//
LOCUS       DAOSDS010000191         1620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_1825
            NODE_25314_length_1620_cov_4.007029, whole genome shotgun sequence.
ACCESSION   DAOSDS010000191 DAOSDS010000000
VERSION     DAOSDS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863354
            Sequence Read Archive: SRR4236667
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 23:04:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,430
            CDSs (total)                      :: 2,394
            Genes (coding)                    :: 2,384
            CDSs (with protein)               :: 2,384
            Genes (RNA)                       :: 36
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 3 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            Pseudo Genes (multiple problems)  :: 2 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25314_length_1620_cov_4.007029"
                     /isolate="AMR_MDS_1825"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.66 N 104.06 E"
                     /collection_date="2015-12-16"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..1497
                     /locus_tag="PLL88_12150"
     CDS             301..1497
                     /locus_tag="PLL88_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOJ02354.1"
                     /translation="MMMKPTALSEGIFWIGVNDPTTPFFEGLWSIEKEGISYNSYLIK
                     DEKSALIDLCKDNTGSLLMENIKACIDPHQLAYLVVNHMEPDHSGAIKELLEIAPQIT
                     ILGSEKTRDMLASFFGVSDRFQAVSDNETITLGKHTLRFLSTPMVHWPETIMTYEETS
                     GTLFSCDGFGGYGKLERGIFDDEQSDVDYFEQEALRYFSNIVCAFSKPVLKAAEKISA
                     LKVNMVAPAHGLVWHSNPAHILELYTQWSGYAGGNYPKKVCVLHASMYGNTDKVVPVV
                     EKTLQDAGIEFESVDIQKTHISYILPKLWTSAGVAICAPTYEGALFPTMEFVLEMADK
                     KRMTNKKAIYIGSYGWGGGAMRFINKQCEVLAWDLLGSVEFKGQAREADIHKIKALSE
                     VLVEKL"
BASE COUNT          469 a          360 c          395 g          396 t
ORIGIN      
        1 ttcactcaca aatgtgcaat tgcttcttga ctttcaagac ggtatctacc cgactgcgat
       61 atactttgga agaagaatgg aaatggagag atttctcggt cgcgctgctc tctcgaaatg
      121 acattgcggg gagaaaatga gaaaaagcag attgactggt ttagtaggta aatggagaga
      181 tcctttcaat cttgcttttt gtattgctaa ctcttgacat ttaagatggt atctactaaa
      241 aagcatcaaa ttgggaaata tgacttaaaa tgatgtcata tttccattta atcaggagaa
      301 atgatgatga aacctaccgc tttatcagag ggaattttct ggatcggggt caatgatccc
      361 acaaccccgt tttttgaagg cttgtggtca attgaaaaag aaggtatttc ctacaacagt
      421 tacctgatca aggatgaaaa gagcgcattg atcgacctgt gcaaagacaa caccggctca
      481 ttattgatgg aaaacatcaa agcctgcatc gatcctcatc aactcgccta tctggtggtc
      541 aatcacatgg aacctgacca ttcgggtgct atcaaagaac tgcttgagat cgccccccag
      601 atcaccattc tgggcagcga gaagacacgc gatatgctgg catctttctt cggcgtgagc
      661 gaccgttttc aagccgtttc ggataatgaa accatcacgc tgggcaagca caccctgcgt
      721 tttctatcca cccccatggt gcactggccc gaaacgatca tgacctatga agaaaccagc
      781 ggcacccttt tctcgtgcga tggctttggc gggtatggga agctggaacg ggggattttc
      841 gatgacgagc aatctgatgt ggactatttt gagcaagagg cattgcgcta cttttccaac
      901 atcgtgtgcg ccttcagcaa accggtgctg aaagcagccg agaagatatc tgccttgaaa
      961 gtgaacatgg tcgccccagc gcatggactg gtatggcact ccaaccctgc ccatattctg
     1021 gaactttata cgcaatggtc agggtatgcc ggtggaaatt accccaagaa ggtctgcgta
     1081 ctgcacgcat ccatgtacgg gaataccgat aaggttgttc cggttgtgga aaaaaccctg
     1141 caggatgctg gcattgaatt cgaaagcgtg gatatccaga aaacgcatat cagctacatc
     1201 ctgccaaaac tatggaccag cgccggagta gcaatatgcg cacccaccta tgaaggggcg
     1261 ctcttcccca ccatggagtt tgtgctcgaa atggcagata aaaaacgcat gaccaacaaa
     1321 aaagcgatct atatcggaag ttatggctgg ggtggtggcg ccatgcgctt tatcaacaaa
     1381 caatgtgaag tgctcgcctg ggaccttctg ggttctgtgg aattcaaagg acaagccaga
     1441 gaagcggata tccacaagat aaaagcgctc agcgaggtcc tggtcgaaaa actgtagatc
     1501 agatttcaag cggacgcatt gccatttctg caaccatgcg attttttctg cgtatatgaa
     1561 atagattacg acgtagaaaa aaggagaaaa aatgaaaaat cgaagtgatc tagtttgggg
//
